LOCUS       DAOMSU010000001        33982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_19_length_33982_cov_2.702302, whole genome shotgun sequence.
ACCESSION   DAOMSU010000001 DAOMSU010000000
VERSION     DAOMSU010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 33982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33982
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19_length_33982_cov_2.702302"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            186..890
                     /locus_tag="PK077_00005"
     CDS             186..890
                     /locus_tag="PK077_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN22714.1"
                     /translation="MIRLRDITKVYHLGEIDLQVLKGITLDINEGEFVSLTGASGSGK
                     STLMNILGCLDRPTSGHYYIAGEDVAKFNAAERATLRNKRIGFVFQNFNLLSRTTALE
                     NVMMPAVYAHPALSMKAMRERSVELLNLVGLSDRMDHTPAQLSGGQQQRVAIARSLIN
                     TPSILLADEPTGNLDSTTSKEVLHMFKDLNRQQGITVILVTHDPKIAAFTDRAINMAD
                     GLICEGISDMLSKDDV"
     gene            887..>1600
                     /locus_tag="PK077_00010"
     CDS             887..>1600
                     /locus_tag="PK077_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN22715.1"
                     /translation="MKFLPLLKTCIRALVRNPMRAALTILGIIIGIAAVIAMVEIGQG
                     STLQIKNTIASMGADTLNIRPGAISKSGVNTGAGGRASLTNADCEAIAKDCPMVLRAT
                     PVVRASGQVIYGNKNWSPETVEGGSVEYLKIKSWYDMARGQPFSEEDVEQARRVCVIG
                     QTVAKELFGDEDPLGKDIRIKNVMFKVIGILQKKGANMMGRDQDDSIILPWTSIRYRL
                     QGLGGGSTTTSTGNSTTTFN"
     assembly_gap    1601..1610
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1611..>2124
                     /locus_tag="PK077_00015"
     CDS             <1611..>2124
                     /locus_tag="PK077_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22716.1"
                     /translation="SAAAVCGAAAALTACGGSASTSSGKSTTTFNRADKYTASSVDYY
                     TETTDQPYTDAPHPRRFNNIDSIMAQISDPERSSEAIDQITEVIRAKHNLKDGQLDDF
                     RVWDMAEMSRAMSSTTEVMTNLLMIVAMISLVVGGVGIMNIMLVSVTERTKEIGLRMA
                     VGARPQDIMRQ"
     assembly_gap    2125..2175
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2176..2356
                     /locus_tag="PK077_00020"
     CDS             <2176..2356
                     /locus_tag="PK077_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22717.1"
                     /translation="MLGKAISIIVSRTMNWATASSPEAMALAVGVSVFIGLAFGWYPS
                     WKASKMDPIDALRHE"
     gene            2378..3751
                     /locus_tag="PK077_00025"
     CDS             2378..3751
                     /locus_tag="PK077_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HRN22718.1"
                     /translation="MSSTFKHLPLLAACFLGSCMVGPDFRTPTNDLPATWEANRPPHS
                     SDKDLRSWWNIFGDPQLNRLVSTSLNNNPDMKVALLRIREAREKLRVSQASLLPSADA
                     SAGWSLSPDRGFRSSTSQNFSLGASTSWELDLFGGNRRSIEASRASLMSTEASAGAVR
                     TALLADVATAYFDWITACEQLRIAREQLEIQRSTLTIAEKRYKTEFAPRLDVEQATST
                     VSSTESRIPQLEAQVASSKNQLAVLLGTYNSRVELTLPKASVFEKTPVVPVGLPSELL
                     RRRPDVIAAEADLHAAVANVGVAVADLYPRFSLTGSLSSRGGDFGQLFRENNNAWSLG
                     GNLLQPLFRGGALRATVRAQKAAAEQAAETYRKTLITAVSEVEDALIAYGSYTSQMQY
                     LHKENNANREAFQLSRTLYINGETDFLNVITAQRSWLSSEESLVTMKQNIRKAVVQLA
                     RALGGGW"
     gene            complement(3957..5093)
                     /locus_tag="PK077_00030"
     CDS             complement(3957..5093)
                     /locus_tag="PK077_00030"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HRN22719.1"
                     /translation="MNEYRQITEAFQENFRNYGELGASVSVWKEGREIVSLHSGFAAA
                     DKTRPWTEHSLVPVYSATKGVASAALLLALHQQGASPQMSMGELWPEFPLPYASIAQM
                     MSHQCGLAAFSRTASVFDHEDCVRALEETVPAWQPPEHGYHPHTYGVMLDELMLRLTG
                     ERLWRYWEEYIRKPLNLDFYIGLPESEWNRVATLYPGKMDMSNMGTPFYLEYMNKGTP
                     VNRAFNTLIGLNSVRQMNTPEAWVSGVPAFGGVASARGMAQFYQACLGLDELRVFPSD
                     VRGWMRNVVIDGPDLTLKTPTAFSCGFMHDPADPATGRKLRHLFGSGGFGHAGAGGSH
                     AFADPAHGLSFGYAMNRMDLNVLPGVKTRNLISAVMKEVAGECS"
     assembly_gap    5190..5199
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5279..8455)
                     /locus_tag="PK077_00035"
     CDS             complement(5279..8455)
                     /locus_tag="PK077_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRN22720.1"
                     /translation="MSAFFIKHPAIAAVIAIVTTLLGLVCMFNLPISQYPEITPRTIQ
                     LQAMFPGASAQAVADSVGTPLERQISGVQGMDYMTSVSSNNGVYNLSVIFEPGSDTDI
                     DQVLTNMRYGQASSQLPQEVQSTGVTIKQQPGLPLMIYSLTSPDGSYHSVDLANYAQV
                     KLVDELKRVEGVGEVQVYGAGRYAIRIWLDTSKMTHYGVSVNEVRGAISAQNTTNPGG
                     KIGADPVPDGQEQTITVRTQGRLSEPHEFEDIIIRQNGDEILYLKDIAKVELGAEDYS
                     ATGRLNGEVSAAIVIFQSPGSNAIATADRVEKLLEAKAPLMPEGITGRVSLDTTTAVR
                     YSIDEIKHTFMEAVILVALVVYVFLQNWRATLIPLIAVPVSLISTFCLFPVLGFSLNT
                     ISLLGMVLAIGLVVDDAIVVVEAVQEHIDKGLNPRMASFAAMQEVSGPVIAIALVLAA
                     VFLPSLLLEGITGTLFKQFAVTIAISMLISAFNALTLSPALCALLLKPRNAGRKSLFS
                     PFHRLFNWCYGRVSNGYTRMCGSLARKLAISIPLLLLFWGAVAPVAERVPGGFLPDED
                     QGFLLACLILKPNTSLQVAYEQDKKFEAALQDPAVKNLTTVVGLNILNSVQTPGACIA
                     FIELKDWSERPETSAELAGKLQGKLAQAGLDGMAMVLEPPAIPGVGTANGVTMVLEDL
                     EGQGVAYLHEQVQRFQEAASRRPEIALCMDMMMADMPQKYVNLDKEKCKFHKVDIDAA
                     NGILASYNGSSFINYFNAFGQQWQVYIQAQGEDRASLEKMDGFFVTNADGARVPLSAL
                     VNIREIEDTEFVMHHNIYNAAKLNVMPRPGHSTQQVMDALEEVFHQTMDPTKVGFDYQ
                     DMSFQENKVRNSIGLGAIFTMSAVFAFLILVALYEKWSLPLAVFLTVPIAVLGAYAGL
                     FWQGMELTLYAQIGLVMLVGLAAKNAILIVEFANLEMQRGKGLMEATLAAARLRLRPI
                     LMTSLAFVLGCIPLMLSSGSGALARNAIGTVVVIGMGVATLVGAFLIPCSYVFIMRLF
                     RIKFSLNDLKEDPDEVGARKYLAAHTKD"
     gene            complement(8459..9799)
                     /locus_tag="PK077_00040"
     CDS             complement(8459..9799)
                     /locus_tag="PK077_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRN22721.1"
                     /translation="MKAHYILIAALCGLTGCREDNGAADAADRREMPPLKVSVMNMEQ
                     RTVELKSSWFGHLRGVEQADIRPQVSGTLIRQVYWDGSICDKGELLFEIDPATYQAAV
                     DREAANLATARAVKLQAEASLNRVQKDLDRYDKLIRTGAISVKNLTDAQQNLKEMQAA
                     LEQAEAGIKQAEASLENARINLDRTKVRAPFRGLASKATASVGELISASGSPLTTMSS
                     IDPIRVDFSVTGKQVLSRLMDGTVNVKTGRMGDMPDFELILEDGSVFEKKGKVVSVDS
                     EVSRSTGTVNFIGHIPNPDLKLRSGMAVRVRAVTGTEKDALLVPARALLSSMNHRNIM
                     VVAPDGTPRRIDVQPGETVVLDMPDGKGGTAPMLMQIVTGTVKPIPESLKEIGYGKPA
                     DAPVIVEGGMMAAQYSKANSLMREHGATEGFGTVVPVPFIYTAPVSTTPSVTAK"
     gene            complement(9879..10304)
                     /locus_tag="PK077_00045"
     CDS             complement(9879..10304)
                     /locus_tag="PK077_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HRN22722.1"
                     /translation="MADPFTISFLMKMITDRVDMMLTRGLTLERVTASQGRVLAYLMS
                     RDGENVSQRDIERHLGVSHTTAKGIVQRLEQKGWVTTAFDSEDGRLKNVYLTDMSRGV
                     HEAICQQIGVIENTLLDGVPEESRSMLRDLLRRMYDNLK"
     gene            complement(10598..11287)
                     /gene="tsaA"
                     /locus_tag="PK077_00050"
     CDS             complement(10598..11287)
                     /gene="tsaA"
                     /locus_tag="PK077_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HRN22723.1"
                     /translation="MQPVARVSTCYPDKFGAPRQSGLVPDARARLVFEPPFRHPDAVR
                     GLEGFSHLWLVWVFSENVDKGWSPTVRPPRLGGNVRMGVFATRAPFRPNPIGLSAVRL
                     ERVELHPLDGPVLHLSGVDLVDGTPILDIKPYVPLADCIPEASEGFTAVPYERLDVEI
                     PESFGGSMSPEERSALEDTLSLDPRPQYQDDPERVYGLLFSGWEVKFKVEGKRLCVLS
                     LERRRSGSGLF"
     gene            complement(11344..12654)
                     /locus_tag="PK077_00055"
     CDS             complement(11344..12654)
                     /locus_tag="PK077_00055"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRN22724.1"
                     /translation="MKTSVLIRNARLTDGSAPADLLVSGGVITTKAPAGTLLESAAEK
                     ALDASGKLVLPGLFDIHAHLCQPGREDKELLLIRFLHLAFERVATATAAALHGGVTGL
                     MAMPDTEPVMDNAAQITTFMEICRTDALVEVIPSGCLTKGDGGEEQASYDSLRAKGVR
                     FITDGDRAPDNMLLLYRAMQYASNLNLTFALRGDVPSLTARAAMHPGTTSYRLGLMGS
                     PSCAEEIGLETIIRLSVDTGNSLHVQTVSTAGSVDILRRCKRKTAGLSAEAALHHLLF
                     THEDVGRYDTCYKTLPPLRDQSDVDALIAAVNDGTIDCIVSDHTPCTPFSKLQDFVVA
                     PQGMIALDTFLPAIYQYLVEPGKMSWQTVVRACSDAPRRLMGLAPVSLEEGAPANLVV
                     FDPEGETPVTDETLRSRARNTPFLGKTLKGKVDAVVFGEQLHRF"
     gene            complement(12661..13590)
                     /locus_tag="PK077_00060"
     CDS             complement(12661..13590)
                     /locus_tag="PK077_00060"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HRN22725.1"
                     /translation="MNHPRKDLLNISSLTDEEIHTLLDSAGPMKELFTKSVKKVPALK
                     GKSVLTLFYEPSTRTRSSFEVAAERLSADVTNFTVSTSSVVKGESVQDTIATLQAMKV
                     DYVIVRHYNSGLPNVIARHTCASVVNAGDGAHAHPSQALLDSFTIREVFPDVRGKRVL
                     IVGDILHSRVARSTSLLMKRLGMEVAFLGPGSLVPRTEHSGIPRFSDFNEAFAWKPDV
                     IYLLRVQKERQDAPFFPSAREYNKIYGVTEERLKRISGEGLYIMHPGPVNRGVEICDL
                     AMDYERCLINRQVENGIACRMSILYHLTPQTQH"
     gene            complement(13619..14152)
                     /gene="pyrR"
                     /locus_tag="PK077_00065"
     CDS             complement(13619..14152)
                     /gene="pyrR"
                     /locus_tag="PK077_00065"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF003549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HRN22726.1"
                     /translation="MTPPSQEIVLDGPGISRALERMAHGIVARFPGEPLAIVGLLSQG
                     DVIARRLVDKVKELGVEAQYGSIDISLYRDDIFKLEARPSLRSSNLPFSTDDMRVVLV
                     DDVLYTGRTIRAALNALFDYGRPARIELACLIDRGGREVPIQPDYTGHVLDHAGAKVI
                     VSMKEADGEDSVVIPSH"
     gene            complement(14179..14589)
                     /locus_tag="PK077_00070"
     CDS             complement(14179..14589)
                     /locus_tag="PK077_00070"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HRN22727.1"
                     /translation="MKVIVIYGSPNDKPFMEPAREYFAQENVPYEETVLSAHRDLPEL
                     IRFLGDLEASGEKAVILAVAGLSAALPGVVVMSCSLPVIGVPVPGGPLNGIDALLAIA
                     QCPGGVPCTTVGLHKKTPVNAAMAAHRILKLAGL"
     gene            complement(14753..15310)
                     /locus_tag="PK077_00075"
     CDS             complement(14753..15310)
                     /locus_tag="PK077_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22728.1"
                     /translation="MSQDIAAPSMANVDLEMEDEKKIAAYRVAMARHLQLGIIWTCIV
                     ELEEKDNRAVLVATLKYIQSPEWAGILDKCPPDYRAMHLKMIRESGKLLERVEREKMT
                     ADEIQNAYGEYGGQYMKMGGSILEKYRLENCSVQFSLYLMRETEGLDDKERLKALYRI
                     RKDILSGKLKVPGEGGGMGESGLEE"
     gene            complement(15610..17172)
                     /locus_tag="PK077_00080"
     CDS             complement(15610..17172)
                     /locus_tag="PK077_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRN22729.1"
                     /translation="MILPMEKAGSERVRRAAVARKLGISPSRIRDLRLVKESIDSRQK
                     KILFQLRLLVGVDSPLPPERLPSRDYPSVRPGSPVALIVGFGPAGMFAALRCLELGVK
                     PVVLERGKDVSSRRFDLAPILRRGTVMEDSNYCFGEGGAGTFSDGKLFTRATKRGPVR
                     EVYEIFVAHGAPREILTDAHPHIGSNLLPNVVKAIRESILRAGGEIHFNARVEHLLRS
                     ADGRRVRGVSCADGREFAADSVLLATGHSARDVYRMMLAEGLALEQKSFAVGVRIEHP
                     QAFVDARQYHLNPEQRRPEQLPAARYSVTTTIQDRGVHSFCMCPGGFIVPAATENDEV
                     VVNGMSLARRDSPFANAGFVVSVHPEDTEPFCREHGVLAGVAYQKALETAACRAGGGM
                     QKAPAQKVEDFLKGRVSSSLLPTSYHPGIVSHPLHELLPEGIVWRMREGLRQFDRKWR
                     GFAGTDAQLIGCETRTSSPVRIPRDPATLEHPGLEGLYPCGEGAGYAGGIVSAALDGR
                     RCAEAMAQQATR"
     gene            complement(17199..17618)
                     /locus_tag="PK077_00085"
     CDS             complement(17199..17618)
                     /locus_tag="PK077_00085"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRN22730.1"
                     /translation="MDDMVRFVCRDAVVSIVEKDSSSPHYRALAENNERLQDLRCVDG
                     SGVEVIPLPMPDPLHAEDWRLDQLPASYANFLIVNEAVIVPVFNQPRNDDRALGILRE
                     CFSGKQVIGVDARKLVLEGGAIHCITQQQPRPGKEGL"
     gene            complement(17579..18253)
                     /locus_tag="PK077_00090"
     CDS             complement(17579..18253)
                     /locus_tag="PK077_00090"
                     /inference="COORDINATES: protein motif:HMM:NF016274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HRN22731.1"
                     /translation="MNKEPDVRWPAEWEPQDAVWLSWPHRRDLWQGGLDELQQTYGSV
                     AAAIAPHALVCVNAAAPLHPGVRQAMLAAGVSEEQFRLFNHPTNDVWCRDHGPVFVQD
                     VKDGSLMLADWQFNAWGGKFAPWDLDNGVPALIGAALGLPVRSSSLILEGGAIEGNGD
                     GLLVTTESVLLNPNRNLDWSRAMIEEELGRMLGVRAVSGSVPALKGMIRMATLTTWCV
                     LCAGMP"
     gene            complement(18270..18665)
                     /locus_tag="PK077_00095"
     CDS             complement(18270..18665)
                     /locus_tag="PK077_00095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22732.1"
                     /translation="MTTPTFEDAVSRIVRKDPRFAERAYSFLKDALDFTMQRVEEREN
                     GSQRHVSGQELLEGFRDYALAQFGPMASTVMKEWGLRNGKNVGEMVFLLIEEDVFSKQ
                     PEDSLDDFKGFMSFRKAFEEPYEFQDQPS"
     gene            18997..20316
                     /locus_tag="PK077_00100"
     CDS             18997..20316
                     /locus_tag="PK077_00100"
                     /inference="COORDINATES: protein motif:HMM:NF036497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRN22733.1"
                     /translation="MFRSSIMLLATMLCVSCVSHRPIQDSSSPPIDAANPLDGTPVAL
                     AWSSGTQLMMGVDTGAVQTSLLFSPAVESIGARLRGRGAMRTANVPVSLKDDGEPISR
                     KQDVVMADQAPYDGLLGWECIRKYVWNINYPKRSHRFFNKLPSRIKSWNKLSLIPGSD
                     YPQIADKHGRRIILDTGAPHAVYISKKRWNAIKQAYPDAFVSVYSGYSPAAGGFYAHE
                     CMHVSSFQLGPLELKNILLCESFANPEVMGIPDDIDIILGYGALAARQFWLDGPGNAL
                     YFSSTSHRMPAPASFNLMGGTFIQDSNGNGPMKAYVAEWSPAWDAGLRTGDVLISING
                     RKNPYPDLVEYVTTQRGAQASVVVQRRNRLVRIQWEVPAAPPAGDYYPTPQAITEQEF
                     ENHVRQQEKKEQTQPSADGQQPPATAGETPDEASPAADGKTDKASAA"
     gene            20352..21662
                     /locus_tag="PK077_00105"
     CDS             20352..21662
                     /locus_tag="PK077_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRN22734.1"
                     /translation="MNNEQHQNTQTSAALAIPSPFQKKTCWHALTGASILVMLGIAAF
                     VIFEVVELLGFLEPVLLPILIAAVVAYLLEPIVSWLVRLKFSRPWAVVTVMFAALAVL
                     VGFGATILPPLIRQTDELIDNRMELWDKTSELIDSTIEIPFISRTIDSVYSTSLRELN
                     ASHYTEAEVHDLRNARTAREKLGAYMTINSSFYQDKLMSWLTSGGRALYSTIGIMVSI
                     LITPIFAFYFLLEADKIKEKWPSILPLKVSKFRKDVVDTMEEINGYLISFFRGQMLVS
                     IIEGILIAICLKLIGLPYAITIGAAVCVLGIIPYLGIITAFIPAVLLAWFTWGDFQHV
                     LIVSGIFLAVNQFDGWIIQPKIVGDSVELHPLTVMFSVLIWTLILGGLIGALLAVPLT
                     AAIKVLYKRYIWQNASMRPMTEPVLPPEHPGEQPPDTPEDSAHA"
     gene            21722..23302
                     /locus_tag="PK077_00110"
     CDS             21722..23302
                     /locus_tag="PK077_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRN22735.1"
                     /translation="MNKSALHLMLALAAAAACPAAVTPTVKPPKAIVMIYADDLGYGD
                     VGCYGAKGIPTPSIDKLAEQGCRFTDAYSTTSVCTPSRYALFTGEYPWRKEGTGILPG
                     DAALIIDTKKPTLPKMLQSHGYKTYMIGKWHLGLGEKGKKIDWNKHISPSPNEIGFDE
                     SFIFAATGDRVPCVILENGNVRNLDPNDPIEVSYRHNFPGLPNGKDNKDQLKLMWSHG
                     HNQAIINGIGRIGFMKGGKSALWKDEENADVITDKAIEYIQKSAKAKEPFFLMFATHD
                     IHVPRCPEKRFVGKSQHGVRGDVTVELDDCVRRITEALQQAGLEKDALVIFSSDNGPV
                     LDDGYRDFAVRDNATHSPAGPFRAGKYSILEGGSRIPFIVKWPGVINPGTTSKALLNQ
                     MDLGASLEQLLAPGKANSFRDSENVLPALLGKSAKGRDYHVINSTGKALAIRHGKWKF
                     IPAGVAIRDGINGASAKMSKSPEGGSLFDLEKDPKELDNVASQHPDICEQMKAKLEEI
                     RQRPETKADQEDLLPLDD"
     gene            23322..23810
                     /locus_tag="PK077_00115"
     CDS             23322..23810
                     /locus_tag="PK077_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22736.1"
                     /translation="MRESATCSQCGHGYTYPAGLDAKTGMRCPACGVLIHVTTGLPFG
                     EASSSPSGRKPENAMKTQAALPPVRKKRPAFSGRDTDERGIRQENLPQLPAAASGRRI
                     PATSWLAWGLSLIALILAFIFPSYGNGSTDPLYMLQCLPSMVFGCTAAILFIQGLAAL
                     RR"
     gene            23964..25214
                     /locus_tag="PK077_00120"
     CDS             23964..25214
                     /locus_tag="PK077_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HRN22737.1"
                     /translation="MEREAEAKDNLWRARLDAVLMHRIWGSLIFLGIVYIIFFLSFAI
                     GDPLVRLIQTGTQLFSVWASRMLEPWPRLQSLLGEGVVGGVGGVLAFLPNVVLLFGAI
                     TVLENSGYMMRVSRLMSRIMKIMGLNGSSFAPLLLGFGCSVPAILSTRRIETRSDRLV
                     TIAVLPMMSCAGRLPIYMMFVSALFPSRLQAPVLFGIYASGVLLALCCARLLKNTLFK
                     APQRDSLHHMKRLRLPSLRKVGILMWSRAFMYVRKAGTFILGASIILWFLNTYPRPEE
                     DTAPTNAAAMEHSYAGQIGHWMEPVTQVAGFDWKINSALVGAFAAKEIFVTQMGILYA
                     VEDGNSAPAAQQTLNARLKASYTPLQGISIMIFCLIALPCIGTVTVAKREAGTWWFAL
                     AQFGGLTLLGFATATLIYQAGLLL"
     gene            complement(25540..26646)
                     /locus_tag="PK077_00125"
     CDS             complement(25540..26646)
                     /locus_tag="PK077_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRN22738.1"
                     /translation="MFRGGVRAYENGSDRKMLVPLEEGVNGPRGETPVSGAACRLFRL
                     MQRYSPVTASRIRLICSATGVGGASIGSLGRGTGAYNRILDDLKAAKILADREGKTLS
                     MPAFIWTQGETDHQDRKTREWYREKMEKLIRDINHDARAVTGQKNDVVCFGYQVGSHL
                     NYYQFNPTDYPAIALEQLDMALEKDSRYVMTTPMYHFEYSDGVHLTAPMSRLYGEYVG
                     YVMKKVLLDGADWKPVHPLNHKIRKFGKGWTVEVAFYAPCPPLVLDTKTVDDPGNYGF
                     SLVGAEGEDIAIKSVRLVGKNAVQLLTEKDPGKGRLRYGMTINEHRPSGPRTGARGCL
                     RDSQGDEVKAHIQGKDYRMDNWCPFFDYSLSKGH"
     assembly_gap    27238..27247
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27248..27851
                     /locus_tag="PK077_00130"
     CDS             <27248..27851
                     /locus_tag="PK077_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRN22739.1"
                     /translation="QRHALLKQTLAETPCQLLFIGDSITHRWETDGKKIWSQYFSPYA
                     PVNFGIGGDRTEHVLWRIDDSALKTPHSPQVCVIMVGTNNTGQYKGRQTPQETAEGIR
                     EIASRVHRLHPATEIILLHIFPRGKTAEDPLRIQNEMINRELDKTNMPRVHVVNINSA
                     FLDKDGTFLPGITGDPVHLTEKGYRLWADALLPEIKKYMK"
     gene            complement(27999..30308)
                     /locus_tag="PK077_00135"
     CDS             complement(27999..30308)
                     /locus_tag="PK077_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22740.1"
                     /translation="MTDQTGTPSSPEPPKLRRRLVTAAPAAAPKVKFVSPAAEGVEVV
                     KPRFLTPELQAKLAQEQEEAARRAAEQAEADRIAAEKAAAEQEAARIAAEKEAARIAA
                     EQEAARIKAEQEAAEAARIAAEKEAARVAAEEERARLAAELEKKEAASAPQPEPSVDA
                     QIQDALAKVAEAQKALAEAQAAALAQAAGVAPAAVQTPEPSVPEPAPAVGAPKLKVFK
                     TDAAPAAAEKSPAAAPESAVPKLKVLKSPVGAAPASSMAPAAVPQPVAAAGASPASGA
                     PPAGDGGIQPPAGTESARDMAASVDKSKSLLRGVLYGVCALVVIAAGVGIYAWSTSSK
                     KEKVEGENARLNALVVEGGKLGRSRLFDSKNLSRVPDVKVVPDAEDAALLLANVRGVK
                     GNRENWPGAAHLVSIMAQMDDNIARQVVEDMKKNVGRYSKEKYSMMVTLLAKSSSPAM
                     RDMLKDLYASISESKNKKIQDKQAVVLKYMRFAMKLDDLDDVMKILQKKDASPDLISS
                     AFRTARYLIDEAPPAKRKALSSKLLQYQKNMPEENVKMLYKLLARTGDPKVLDMMEKS
                     YKEDPKKALAIITAWGDWNTDDAVPYLFKAWKDESLHERVRSQAHDSILRVLSVDRDR
                     DDNATLKLFDPLIADAKTSERRQFLVSAFKRLSNRPYVIRLLGRIKQTAEDQRNAVEP
                     KFQAAEEALFKAEDKFKANPGDAVAKADYEAKEKIYNELSSQKTGEDKVIAAVDKALE
                     KVRKTPVPTRKAASAEDRDDDDSSVIKTI"
     gene            30680..31060
                     /locus_tag="PK077_00140"
     CDS             30680..31060
                     /locus_tag="PK077_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22741.1"
                     /translation="MNSNDSTKNKRRYTPEEKQSVLDFIAERKTQNKRGAQKEAAERF
                     GISTVTISSWMKASKAKRGRKPGTKNTTAPKTQPLAAAQPADLRRLAAVLEEIADLEA
                     QTAKLDTLRTEAEELKNRLTAKQA"
     gene            31178..33319
                     /locus_tag="PK077_00145"
     CDS             31178..33319
                     /locus_tag="PK077_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HRN22742.1"
                     /translation="MTQPAHIAPASQSVEGMYADYFLDYASYVILERAVPKINDGFKP
                     VQRRILHAMDRLDDGRYNKVANIVGDTMKFHPHGDRSIADALVGLGQKGLLIDTQGNW
                     GNILTGDPAAASRYIEARFTSFARDVVFSPKVTEWQLSYDGRNKEPVSLPVKFPLLLA
                     QGAEGIAVGLSSKILPHNFNELIEASIAYLRGQPFQLLPDFPTGGVMDATNYRDGERG
                     TGRVRIRARILTESKKLLRITEIPFGVTTEILIDSIVSAAEKGKIKIARIEDNTAQHV
                     DILVHLPAGADPEQTRKALFAFSACEVSISPNACVIVEEKPRFMCVSDILRYNTDSTK
                     EILRQEQEIRLKELNEAWHQASLEKIFIENRIYLSIEDSETWEEVLGTIDRELQPFAS
                     RLRAPITRDDLVRLTEIKIKRISKFDAFKADQHIRQLEEDIEQTQKNLNQLTKFTIRW
                     FEALRKKYGAAYPRKTEISSFGSVNRAQVAVANETLYIDDEGFAGYGVKKGNPVCKCS
                     TLDDVLIIDNAGVLKIVRIQDKFFAGKNPLYISVIKKGDDPVFNLIYRDGKDGPVYAK
                     RFRIGGFTRDKEYPLTRGAKGTRIFHFSVHETEENSSQISVNVYLKAVLKLRNLIRPF
                     HFADLRIKNRGAQGNIITRHPVERVSRIMPPAKSGNEETEEPTTAPSATAERTEGSPA
                     PSAETLHPETAPHLEEPPADPPLEQGSLFDS"
     gene            33423..33497
                     /locus_tag="PK077_00150"
     tRNA            33423..33497
                     /locus_tag="PK077_00150"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33455..33457,aa:Thr,seq:tgt)
BASE COUNT         7648 a        10518 c         8969 g         6766 t
ORIGIN      
        1 cctccgccgg cggggaaaac ccgttcgcgc ccaaaatgcc cccgcgccgc aagcctagca
       61 caggcaatac aaaaacgcca acctcggccg gaaacgatgg ccctcccccg cctccctgac
      121 ccggggaacc gccatcattc gacctcttaa ttactctttt tcaccgcatc aagcattcca
      181 acaacgtgat ccgcttacgt gacataacca aggtgtacca cttgggagaa atagaccttc
      241 aagtgctcaa gggcatcacc ctggatatca acgaggggga attcgtctcc ctcacaggag
      301 cttccggttc aggaaaatcc accctgatga atatcctggg ctgtctggac cgccctacct
      361 caggccatta ctatattgcc ggggaagatg ttgccaaatt caacgccgca gaacgagcca
      421 ctctccggaa caagcgcatc ggattcgtct tccagaactt taacctgctc tcccgtacca
      481 cagccctgga aaacgtgatg atgcctgccg tatatgccca ccctgccctc agcatgaaag
      541 ccatgcgcga acgttccgtg gaattgctga atttggtagg cctttccgac cgcatggacc
      601 acaccccggc ccagctttcc ggcggacagc agcagcgcgt agccattgcc cgttccctca
      661 tcaacacccc cagcatcctg ctggcggacg aaccaacggg aaacctggac tccactacct
      721 ccaaggaagt gctgcacatg ttcaaggacc tgaaccgcca acagggaatc acagtcatcc
      781 tggtgacgca cgaccccaaa atagccgcct tcacggacag ggccatcaac atggcggacg
      841 gtcttatctg cgaagggatt tccgatatgc ttagcaagga cgacgtatga aattccttcc
      901 tctccttaaa acctgcatca gggccctggt gcgcaatccc atgcgcgcag ccctcaccat
      961 cctgggcatc atcatcggca ttgccgcagt aatcgccatg gtggaaatcg gccagggatc
     1021 tacgctccag attaaaaata ccatcgcttc catgggggcg gacacgctga atatccgccc
     1081 tggagccatc tccaaaagcg gcgtcaatac cggcgcgggc ggacgggcct ccctgaccaa
     1141 cgcggactgc gaagccattg cgaaagactg ccccatggtt ctgcgggcta cccccgtggt
     1201 gcgcgccagc ggccaggtca tttacggcaa caagaactgg agcccggaaa ctgtagaggg
     1261 cggttccgtg gaatacctga aaatcaaaag ctggtatgac atggcgcgag gccagccctt
     1321 ctccgaggaa gatgtggaac aggccaggcg cgtgtgcgtc atcggccaga cggtggccaa
     1381 ggaacttttc ggggacgaag acccgctggg caaggatatc cgcatcaaga atgtcatgtt
     1441 caaagtcatc ggcatccttc agaaaaaagg ggccaacatg atgggacgcg accaggacga
     1501 ctccatcatc ctcccatgga caagcatccg ctaccgcctc cagggcctgg gcggcggttc
     1561 caccaccact tccaccggca acagcaccac caccttcaac nnnnnnnnnn tctgccgccg
     1621 ctgtctgcgg cgcagctgct gccctgactg cctgcggcgg ttccgcctcc acttcctccg
     1681 gcaaatccac caccaccttc aaccgggcag ataaatacac tgccagttcc gtggattact
     1741 acacggaaac tacggaccag ccctatacgg acgctcccca tccgcggcgc ttcaacaata
     1801 ttgattccat catggctcag atttcagacc cggaacgctc ctccgaggcc attgaccaga
     1861 ttacggaagt gatccgtgcc aaacacaacc tcaaggacgg ccagctggac gatttccggg
     1921 tatgggacat ggcggaaatg tcccgcgcca tgagcagcac cacggaagtg atgaccaatc
     1981 tcctgatgat cgtggccatg atctctctgg tcgtcggcgg cgtcggcatc atgaatatca
     2041 tgctcgtttc cgtcacggaa cgaaccaagg aaattggcct gcgcatggcg gtgggcgccc
     2101 gtccgcagga tattatgcgc cagtnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnncatgc tcggcaaggc gatctccatc atcgtcagcc gcaccatgaa
     2221 ctgggccaca gcctcctccc cggaagccat ggctctggct gtaggcgtct ccgtattcat
     2281 cggcctggcc ttcggatggt acccctcctg gaaggcgtcc aagatggacc ccattgatgc
     2341 ccttcgccac gaataacacc ttacttttta tttttccatg tcatccacgt tcaaacatct
     2401 cccccttctg gccgcctgtt tccttggctc ctgcatggta ggcccggatt tccggacgcc
     2461 gaccaatgac ctccccgcca cgtgggaggc gaacaggccc ccccacagtt cggacaaaga
     2521 tttgcgttcc tggtggaata tctttggaga cccccaactc aaccgcctgg tctccacctc
     2581 cctcaacaac aatccggaca tgaaggtggc tttgctccgc atccgggagg ccagggaaaa
     2641 gctccgcgtc tcccaggcat ccctgcttcc ctccgcagac gccagcgccg ggtggagcct
     2701 gtccccagac agaggttttc gcagctccac ttcccagaac ttctccctgg gagcgtccac
     2761 ctcctgggag ctggacctct ttggcggcaa ccggcgttcc atcgaggcct cccgcgcctc
     2821 cctcatgtcc acggaagctt ccgcaggagc cgtgcgcaca gccctgctgg cggacgtcgc
     2881 tacggcctac tttgactgga ttaccgcctg cgaacagctc cgcatcgcca gggaacaact
     2941 ggaaatccaa cgcagcaccc tcaccattgc ggaaaaacgc tacaaaacgg aattcgcccc
     3001 ccgtctggat gtggagcagg ccacctccac cgtctcctcc acggaaagcc gcatcccgca
     3061 actggaagca caggtggcct cctctaaaaa ccagctcgcc gtgctgctgg gcacctataa
     3121 ttcccgtgta gaactgacgc tgcccaaagc ttccgttttt gagaaaacgc ccgtcgttcc
     3181 ggtaggcctc ccctccgaac tcctgcggcg caggcccgac gttatcgccg ctgaggcgga
     3241 cctgcacgcg gccgtggcca acgtgggcgt agccgtagcg gatctgtacc cgcgcttcag
     3301 tctgacgggc tccctttcca gccgcggcgg agatttcggc cagctcttca gggaaaacaa
     3361 caacgcgtgg tccctgggag ggaatctgct ccagcccctg ttccggggcg gagccctgag
     3421 agccacggta cgcgcccaga aagccgcagc ggaacaagct gcggaaactt accgcaaaac
     3481 gctcattacc gccgtctccg aggtggagga cgccctgatc gcctatggca gctatacatc
     3541 ccagatgcaa tacctgcaca aggaaaacaa cgccaacaga gaagcgttcc agctctcccg
     3601 caccctgtac atcaacggcg agacggactt cctgaacgtg atcaccgccc agcgttcctg
     3661 gctcagctcg gaggaatccc tggtcaccat gaagcagaac atccgcaagg ccgtggtgca
     3721 gctcgcgcgc gccctgggcg gagggtggta accggcagct ccctccaaaa ggccggggtc
     3781 gccatccccg gtaaacggaa ccctgatttt tcaacagggg acagggcagc aaggaaatga
     3841 ctgaagaatc attctttcgc gcccttcaca ttcagattaa aaaagaccgt aagaatagga
     3901 acgaataaac tatccggaaa cttccgatgg aattgaacag atagccgggg ctcaggttaa
     3961 gaacactctc cagccacttc tttcatgact gcgctgataa ggttccgggt cttcacgccg
     4021 ggcagcacgt tcaagtccat gcggttcatc gcgtagccga aggaaaggcc gtgggccgga
     4081 tccgcaaagg catgggaacc gcccgccccg gcatgaccga agccaccgga gccgaacaga
     4141 tggcgcagct tgcggcccgt ggcggggtct gcgggatcgt gcatgaagcc gcaggaaaag
     4201 gcggtgggcg ttttcagcgt caggtccggc ccgtcaatga cgacgttccg catccagccg
     4261 cgcacgtcgg acgggaatac ccggagttca tccagcccca ggcaggcctg gtagaactgc
     4321 gccatgccgc gggcggaagc cacgccgcca aaggccggaa ctccggacac ccacgcttcc
     4381 ggcgtattca tctgccgcac actgttcagc cctatcagag tattgaacgc acggtttacg
     4441 ggcgtgccct tgttcatgta ttccagataa aacggcgtac ccatgttgga catgtccatc
     4501 ttgccgggat acagcgtcgc cacgcggttc cattcggatt ccggcaaccc gatgtaaaaa
     4561 tccagattca acggtttgcg gatgtattcc tcccagtagc gccacaggcg ttccccggtc
     4621 agccgcagca tgagttcatc cagcatcacg ccgtaggtat gcggatggta gccgtgttcc
     4681 ggcggctgcc acgccggaac ggtttcctcc agggcgcgca cgcaatcctc atggtcaaac
     4741 acgcttgccg tccgggaaaa agccgccaaa ccgcattgat gggacatcat ctgcgcgatg
     4801 gatgcatagg gcagcgggaa ttcaggccac agttccccca tgctcatctg cggcgaggct
     4861 ccctgctggt gcagggccag cagaagggcg gcggatgcga ctcccttggt ggcggagtaa
     4921 acgggcacca gcgaatgttc cgtccatgga cgcgttttat ccgctgcggc aaacccggaa
     4981 tgcagggaaa cgatttccct tccttccttc cagacagaaa cggaggcgcc caattctccg
     5041 tagttacgga agttttcctg aaaagcttca gttatctgcc ggtactcatt cattccctta
     5101 aaatgacccg gcgttactcc cggcgtccgg acaggaaaat actcccctcc ggctccgaaa
     5161 gcaagctccg gcgctgccct ggatggtcan nnnnnnnnnc gcccttgccg tggtctacat
     5221 tgtccacctt gccgctgccc tggatggtca gcgccggaac aggacaatct gtaaaacatc
     5281 agtctttcgt atgggcggcc aggtatttcc tggctcctac ttcatccgga tcctccttca
     5341 gatcattcag ggagaattta atacggaata atctcatgat gaacacataa gaacatggaa
     5401 tcagaaaagc gcccaccagt gtagccaccc ccatgccaat caccaccacc gtaccgatag
     5461 cgttgcgcgc cagagctccg gaaccggagg aaagcatcag ggggatgcat cccagaacga
     5521 atgccagaga agtcatcaga atgggacgca ggcgcagacg cgcggcagcc agcgtagcct
     5581 ccatcaagcc cttgccgcgc tgcatctcca gattggcgaa ttccacgatg aggatggcgt
     5641 tcttggccgc cagacccacc agcatcacca gaccgatctg cgcgtagagc gtcagctcca
     5701 tgccctgcca gaacaacccg gcataggccc ccagcacggc aatgggaacc gtgaggaaaa
     5761 ccgccagggg aagagaccat ttctcataaa gcgccaccag gataaggaag gcgaaaacgg
     5821 cggacatggt gaagatggcc cccagcccaa tactgttgcg cactttattt tcctggaagc
     5881 tcatgtcctg gtagtcgaag ccaaccttcg tgggatccat cgtctgatgg aacacttcct
     5941 ccagcgcatc catcacctgc tgggtggagt ggccgggccg gggcatcacg ttcaatttgg
     6001 cggcgttgta gatgttatgg tgcattacga attccgtatc ctctatctcc ctgatgttca
     6061 ccagggcgga gagaggcacg cgggctccgt ccgcgttcgt gacaaagaag ccgtccatct
     6121 tttccaggct ggcgcggtct tcgccctgcg cctggatata cacctgccac tgctggccga
     6181 aggcattgaa gtaattgatg aaggaggaac cgttgtagga ggccagaatt ccgttggccg
     6241 cgtcaatatc caccttgtgg aatttgcatt tttccttgtc caggttcacg tacttctgcg
     6301 gcatgtccgc catcatcatg tccatgcaca aggctatttc cggacgccgg gatgccgctt
     6361 cctggaaccg ttgcacctgt tcatgcagat aagctacgcc ctgcccttcc agatcctcca
     6421 gtaccatcgt aaccccgttg gcggttccca cgccggggat ggcgggaggt tccagcacca
     6481 tcgccatgcc gtccaggccg gcctgggcca gctttccctg aagctttccg gccagctccg
     6541 cggaggtttc cggccgttcg ctccagtcct tgagctcaat gaaagcaatg caggcgccgg
     6601 gagtctgcac gctgttgagg atgttaagcc ccaccacggt agtaaggttc ttcacggcgg
     6661 gatcctggag ggccgcctca aattttttat cctgttcata ggcaacctgc agggaggtat
     6721 tcggcttcag gatcaggcag gccagcagaa agccctggtc ttcatccgga agaaagccgc
     6781 cgggaacgcg ctccgccaca ggagctaccg ccccccagaa taacaacagg agggggatgg
     6841 aaatcgccag cttgcgggcc aggcttccgc acatgcgggt atagccgttg gagacacgtc
     6901 cgtaacacca gttgaacaaa cggtgaaatg gggaaaagag gctcttcctg cccgcattcc
     6961 ggggtttcag caacagggcg cacaaggcag gggaaagcgt cagcgcatta aaggcggaaa
     7021 tgagcatgga aatggcgatg gtcacggcga actgcttgaa cagcgttccc gtgatgccct
     7081 ccagcagcag ggaaggcagg aacaccgccg ccagcaccag ggcaatggcg atgacggggc
     7141 cggatacctc ctgcatggcg gcgaaggaag ccatgcgcgg attgagcccc ttgtctatgt
     7201 gctcctgcac ggcttccacc accacaatgg cgtcatccac taccaggccg atggccagca
     7261 ccatgccaag aagggaaatg gtgttcagag aaaaacccag gacgggaaag aggcagaaag
     7321 tggaaataag ggaaaccgga acggcgatga gtggaatgag ggtggcgcgc cagttttgaa
     7381 ggaaaacata cacgaccagg gccaccagga tgacggcttc catgaacgtg tgcttgattt
     7441 catcaatgga atagcgcacc gccgtagtcg tatccaggga aacgcgcccg gtaattcctt
     7501 ccggcatcag cggagccttg gcttccagca gtttttccac gcggtccgcc gtggcgatgg
     7561 cgttggatcc cggagactgg aagataacga tagccgcgga aacttctccg ttcaggcggc
     7621 cggtggcgga atagtcttca gctcccagtt ccaccttggc aatatctttc aggtacagga
     7681 tttcatcccc attctggcga atgatgatgt cttcaaattc atggggctcc gaaagacgcc
     7741 cctgagtgcg cacggtaatg gtctgctcct ggccgtccgg cacggggtcc gccccgatct
     7801 tgccgccggg gttggtggtg ttctgggcgg agatggcgcc ccgcacttca ttgacggaaa
     7861 cgccgtaatg ggtcattttg gacgtatcca gccagatgcg gatggcataa cgccccgcgc
     7921 cgtacacctg aacttccccc acgccctcca cgcgcttaag ttcatccacc agcttcacct
     7981 gggcatagtt cgccaaatcc acggaatgat aactgccgtc cggggatgtc agggaataaa
     8041 tcatcagcgg aagccccggc tgctgcttga tggtcacgcc cgtgctctga acttcctgcg
     8101 gaagctggga ggaggcctgc ccatagcgca tattggtcag cacctggtca atatccgtat
     8161 cggaacccgg ttcaaaaatg acggaaaggt tatacacgcc gttgttggag gaaacggacg
     8221 tcatgtagtc catgccctgc acgccggaaa tctggcgttc caggggagtg cccacggaat
     8281 cagccaccgc ctgcgcgctg gcccccggaa acatggcctg aagctggatg gtgcgcggcg
     8341 tgatttccgg atactgggaa ataggcaggt tgaacatgca gaccagcccc agcagcgtag
     8401 tcacaatggc aatcacggct gcgattgcgg gatgcttgat gaaaaacgca ctcatggctt
     8461 atttggcagt tacggaagga gtggtggaaa caggggcggt atagatgaag ggaacgggga
     8521 caaccgtccc gaacccttcc gtggctccat gttcccgcat caggctgttc gccttggaat
     8581 actgggcggc catcatgccg ccttccacga tgacgggagc atccgccggc tttccgtatc
     8641 ctatttcctt cagagattcc ggaatgggct tgaccgttcc cgtcactatc tgcatcagca
     8701 tgggggccgt gccgcccttg ccgtcaggca tgtcaaggac tacggtttcc cccggctgca
     8761 cgtcaatgcg gcgcggcgtc ccgtccggag ccaccaccat gatgttgcgg tggttcatgg
     8821 aagaaagcag ggcgcgggcg ggaaccagca aggcatcctt ctccgtaccc gtcacggcgc
     8881 gcacgcgcac cgccattccg gaacgcagtt tcagatccgg attgggaata tggccgatga
     8941 aattgacggt gcccgtcgaa cggctcacct cgctgtccac ggaaaccacc ttccccttct
     9001 tttcaaagac ggagccgtct tccagaatca gttcaaaatc cggcatgtct cccatgcgcc
     9061 cggttttcac attcacggtt ccatccatca gacggctgag aacctgtttt cccgtcacgg
     9121 aaaaatccac ccggatgggg tcgatggaag acatggtcgt gagcggcgag ccgctggcgg
     9181 aaatcagttc ccccacgctg gccgtcgcct tggaagccag tccccggaac ggcgcgcgta
     9241 ctttggtgcg gtccagatta atccgggcgt tttccaggga ggcttccgcc tgcttgatgc
     9301 ccgcttccgc ctgttccaga gcggcctgca tctccttaag gttctgttgc gcgtccgtca
     9361 ggttcttgac ggaaatagcc cccgtcctga tcagtttgtc atagcggtcc aaatccttct
     9421 gcaccctgtt cagagaagct tccgcctgaa gtttgacggc acgagccgta gccaggttgg
     9481 ccgcctccct gtccacggcg gcctgatagg tagccgggtc aatttcaaaa agcagttctc
     9541 ccttgtcgca aatggagccg tcccagtaaa cctggcgtat cagcgttccg gaaacttgcg
     9601 gcctgatgtc cgcctgctcc accccgcgca aatggccgaa ccaggaagat ttcaactcca
     9661 ccgttctctg ctccatattc atcacggaaa ccttgagcgg aggcatttcc ctccggtctg
     9721 cagcatccgc ggcgccgttg tcttcccggc accctgtcag cccgcacagg gccgctatca
     9781 gtatatagtg tgctttcatg ttgttggtta acaatattat tgaagcatgc ttcatatttt
     9841 tcaaaacctg tttaccgaaa aaaacgcacg ggactttatt atttgagatt gtcgtacatg
     9901 cgccggagca aatccctcag catgctgcgg ctttcctccg gcaccccgtc cagcagcgta
     9961 ttttcaatca ctcctatctg ctggcaaatg gcctcatgaa cgccccggct catatccgtc
    10021 aggtacacat tcttcagccg tccgtcttca ctgtcaaaag ccgtggttac ccagcccttc
    10081 tgttccagcc tctgaacaat tcccttggcc gtggtatggg agacccccaa atgccgctct
    10141 atgtccctct gggagacgtt ttctccgtca cgggacatca ggtaagccag cacccggccc
    10201 tgggaggccg taacacgctc cagagtcaaa ccgcgcgtaa gcatcatatc cacgcggtcc
    10261 gtaatcattt tcatcaaaaa cgaaatcgta aatgggtcgg ccataaaaca atgaagcatg
    10321 cttcaaaaat gtaccgatag gtactcttta tccggacgca agtcaagcgg aatcagaatg
    10381 acagagcgcg gaaaaacacg ttggcctcac cagtaaaaat aaatcaaatc cgttctttat
    10441 caataaactg aaaaaacaaa ccatcaataa tccgaccacc ttcgcatttc tttcccgcat
    10501 cagccggcgt ttcccggcaa acatacccct tccagccaca ggaaaccaat gctccgcgca
    10561 gggataaaga aaaaatacct aaggaatggc ccgcatatca aaaaaggccg gaaccgcttc
    10621 gccttctttc cagggacaat acgcacagcc gctttccctc taccttgaat ttcacctccc
    10681 agccagagaa caaaaggccg taaacccgtt ccggatcatc ctgatactgg ggacggggat
    10741 ccagggacag cgtatcttcc agcgcactcc tctcttccgg agacatggaa ccgccaaaag
    10801 attcgggaat ttccacatcc agccgttcat aggggacggc cgtaaaccct tccgacgcct
    10861 ccggaatgca gtccgccaga ggaacatagg gcttgatatc cagaatgggg gtgccatcca
    10921 ccaaatccac gccggaaaga tgcaataccg ggccgtccag aggatgcaat tccacccgtt
    10981 ccagcctgac ggcagacaac ccgatgggat tgggacggaa cggcgcacgc gtggcgaaca
    11041 cccccatgcg cacattgccc cccagccgcg gaggccggac cgtagggctc caccccttat
    11101 ccacgttttc actgaacacc cagaccagcc acagatggga aaacccttcc agaccacgaa
    11161 cggcatccgg atggcggaac ggcggctcaa ataccagccg cgcccgcgca tccggaacca
    11221 ggccgctctg gcgcggcgca ccgaatttgt ccggataaca cgtgctgacg cgggcgacgg
    11281 gctgcaagcg gagactgata gttaataatt aatagctgcc ggcctttctc cgtgacgctt
    11341 tcatcagaag cggtggagct gttctccgaa caccacggca tccacttttc ccttcaaggt
    11401 cttgcccagg aatggggtgt tgcgggcgcg gctgcggagc gtttcatccg tgacgggcgt
    11461 ttctccttcc gggtcaaaca ccaccagatt ggccggggcc ccttcttcca gggagacggg
    11521 ggccagcccc atcagcctgc ggggagcatc gctgcaggcg cgcactactg tctgccagga
    11581 catttttcca ggttccacca gatactggta aatagcgggc aggaacgtat ccagcgcaat
    11641 catgccctgc ggggcgacaa cgaaatcctg aagtttggaa aacggcgtgc agggcgtgtg
    11701 atcggaaaca atgcagtcaa tggtgccgtc attcacggcg gcaatcaggg catccacatc
    11761 cgactggtcg cgcaggggcg gcagcgtctt gtagcaggta tcgtaccggc ccacgtcctc
    11821 atgcgtgaac aggagatggt gaagcgccgc ttccgcgctc aacccggcgg ttttccgctt
    11881 gcagcggcgc aggatatcca cgctgccggc cgtggagacg gtctgcacat gcagggagtt
    11941 gcccgtatcc acggaaaggc ggatgatggt ttccagccct atttcctccg cacaggaggg
    12001 ggagcccatc aaccccagac ggtaggaagt agtgcccggg tgcatggccg ccctggccgt
    12061 cagggacggc acgtctccgc gcagggcgaa agtaaggttc aaattgctgg cgtactgcat
    12121 ggcgcggtac agcagcagca tgttgtccgg cgcacggtcc ccgtccgtga tgaaacggac
    12181 gcccttggcg cgcagggagt cgtaggaggc ctgctcttcc ccgccgtccc ccttggtcag
    12241 gcaaccggag gggataacct ccaccagagc atccgtccgg catatttcca tgaacgtggt
    12301 aatctgggcg gcattgtcca tcacgggttc ggtatccggc atcgccatca gtccggtcac
    12361 tcctccatgc agggcggcgg cggtagccgt agccacacgt tcaaaggcca gatgcagaaa
    12421 tcggatgagg agcagttcct tgtcctcccg gccgggctgg cacaaatggg catggatgtc
    12481 gaacaggccg ggcagaacaa gtttcccgga agcatccagc gccttttccg cggcgctctc
    12541 caaaagcgtt ccggcaggag ctttcgttgt aatcaccccc ccggaaacca gaaggtccgc
    12601 cggggcggaa ccgtccgtca accgggcgtt gcgaataagt acggaagttt tcatgaacaa
    12661 tcagtgctgg gtttgcggag tgagatggta aaggatggac atgcggcagg cgatgccgtt
    12721 ttccacctgg cggttgatca ggcagcgctc atagtccatc gccaggtcac agatttccac
    12781 gccgcggttg accggccccg gatgcatgat gtacaggcct tcaccggaaa tgcgcttcag
    12841 gcgttcttcc gtgacgccgt aaatcttgtt gtactcccgg gcgctgggga agaaaggggc
    12901 gtcctgccgc tctttttgaa cgcgcagcag gtaaattacg tccggcttcc aggcaaacgc
    12961 ctcattgaaa tcggaaaaac ggggaatgcc ggaatgttcg gtgcgcggca ccagggaacc
    13021 cggccccagg aaggcgacct ccatccccag cctcttcatc agcagggagg tggaacgggc
    13081 cacgcgggaa tgcaggatgt cccccacaat cagaacacgc tttcccctca catccggaaa
    13141 cacctcccgg atggtaaagg aatccagcag cgcctgagag ggatgggcgt gggctccgtc
    13201 cccggcgttc accacactgg cgcaggtatg gcgcgcaatc acgttcggca agccgctgtt
    13261 gtaatggcgc acgattacat aatccacttt catggcctgg agcgtggcga tcgtgtcctg
    13321 tacggattcc cctttcacca cggaagaggt ggacaccgtg aaattggtca cgtccgcaga
    13381 cagacgttcc gccgctacct caaaagagga acgggtgcgg gtgctgggtt cataaaaaag
    13441 cgtcagaacg gacttgcctt tcagggcggg cacctttttg acgcttttgg taaaaagctc
    13501 cttcatcggg ccggccgaat ccagcagcgt gtgaatttcc tcgtcagtca acgaggagat
    13561 attaagaaga tccttgcggg gatgattcat gggaaacgga gaatggattg gaaaattttc
    13621 agtgggaagg aatgaccacg ctgtcctcgc cgtcagcctc cttcatgctt acgatgacct
    13681 tggctccggc atggtccagc acgtgccccg tataatccgg ctggatggga acttcccgcc
    13741 cgccgcggtc aatcaggcac gccagttcaa tacgggccgg acggccgtag tcgaaaaggg
    13801 cgttcagggc cgcacgtatg gtgcgccccg tataaaggac gtcgtccacc agcacgaccc
    13861 tcatgtcatc cgtggaaaac ggcaaattgg aggaacggag ggaaggccgg gcttccagct
    13921 tgaagatatc atcccggtac agggaaatat caatagagcc gtactgggct tccactccca
    13981 gctcctttac cttgtccacc agacgccggg caatcacatc cccctggctg agcaggccga
    14041 cgatggcgag aggttctccg gggaaacggg ccacaatgcc gtgggccatg cgctcaagcg
    14101 cacgggaaat tcccggaccg tccaatacga tttcctgtga agggggagtc atttgccgga
    14161 tggaggaaaa agttcacgtt acagccccgc cagttttaaa atgcggtggg cggccatggc
    14221 ggcattgacg ggggttttct tatgcaggcc aaccgtagtg cagggcacgc cgccgggaca
    14281 ctgggcaatc gccagcaggg cgtcaatacc gttcaggggg cctccgggga ccggcacgcc
    14341 gataaccggg agggaacagc tcatcaccac cacgccggga agggcggcgg acaggccggc
    14401 cacagccagg ataaccgctt tttctccgct ggcttccaaa tcccccagga acctgatcag
    14461 ctccggaagg tcgcggtggg cggacagaac ggtttcttca taaggaacat tctcctgagc
    14521 aaaatactcg cgggccggtt ccataaaggg cttatcattc gggctgccgt agatgacgat
    14581 gactttcatg gctgtgtgtg cggttgtgaa acttgcagat tatgccccca agccccctgc
    14641 cctggcaaga cttaacctgc aaaaaaagga aggccccggg gggaaatgaa caacggaaga
    14701 aaggaaacac cggggttccg gaaccgttcc gggaatcggc ccggcatccc ggtcattcct
    14761 ccaacccaga ttcgcccatc cctccgccct ctccgggaac cttcagtttt ccggagagga
    14821 tatccttcct gatacggtaa agagccttca acctttcctt atcatccaat ccttccgttt
    14881 cccgcatcag gtataaagaa aactgtacgg aacaattctc cagccgatat ttttccagga
    14941 tgctcccccc cattttcatg tattggcctc cgtattctcc gtaagcgttc tgaatttcat
    15001 cggcagtcat cttctctctt tccacccgtt ccagcaattt cccggactct ctaatcatct
    15061 tcagatgcat ggcgcggtaa tcgggagggc atttatccag aatcccagcc cattcgggag
    15121 attgaatata tttcagcgtg gccaccagca ccgcacgatt atccttctcc tccaactcca
    15181 caatgcacgt ccaaataatg cccagctgca aatgcctggc catggccacc ctgtacgccg
    15241 ctattttctt ttcatcctcc atttccaaat ccacattagc catggagggc gccgctatat
    15301 cctgggacat gccggaaaaa gtcatcaaaa acgcagggag aattccccac cggaacagat
    15361 ttgacatgaa accacgttac aggaatcttt tgcgcagggt caacaatatt cttcccttcc
    15421 gccgcatccc gcagcattgg cggcagcctc cacggcggac tgtctttcct tcccggaaaa
    15481 aacaagcctg ccctcatttc ccggcttgat ggcatccggt tccggcataa caaggctcac
    15541 aggggcgtat cttcatccag aaacgcagaa aaactgcatc gctccggcag ccggacgctt
    15601 gaaaaaaacc tagcgggtcg cctgctgcgc catggcctcc gcacaacggc gtccgtccag
    15661 ggcggcggaa acaatgccgc cggcgtaacc cgccccttcg ccgcatgggt acaggccctc
    15721 cagaccggga tgctccagcg tggcgggatc ccttggaatg cgcacggggg agctggtgcg
    15781 cgtctcacag ccaatgagct gggcgtccgt tccggcaaat ccccgccatt tacggtcaaa
    15841 ctgccgcagc ccttcccgca tgcgccagac gatcccttcc gggagcaatt catgaagggg
    15901 atgggatacg ataccgggat gatagctggt aggcagcagg gaagaagaaa cgcgcccttt
    15961 cagaaaatcc tccaccttct gggccggggc tttctgcatg ccccctccgg cacggcaggc
    16021 agccgtctcc agcgccttct ggtacgccac gcccgccaac accccgtgtt ccctgcaaaa
    16081 cggttccgta tcttccgggt gcacgctgac tacaaaaccg gcattggcaa atggagaatc
    16141 cctccgggcc agagacatgc cgttgaccac cacctcatcg ttctccgtgg cggcgggcac
    16201 gatgaagccc ccgggacaca tgcagaagga atggacgccg cggtcctgga tggtggtcgt
    16261 cacggaataa cgggctgccg gaagctgctc cgggcgcctc tgctccggat tcaggtggta
    16321 ctggcgcgca tccacaaacg cctggggatg ctcaatgcgc acacccacgg caaaggattt
    16381 ctgttccaga gccagcccct ccgccagcat catgcggtac acgtcccggg cactgtgccc
    16441 ggtagccagc aaaacggaat ccgccgcaaa ttcccggcca tccgcgcagg acacgccgcg
    16501 tacgcggcgg ccgtccgcag aacggagcag atgctccaca cgggcattga aatgaatttc
    16561 cccgcccgcg cgaaggatgg attccctgat ggctttgacg acattgggca acagattgga
    16621 gccgatatgc ggatgggcgt ccgttaaaat ttccctgggc gctccgtggg ctacaaaaat
    16681 ctcgtaaacc tcccggacgg ggcctctctt ggtcgcgcgc gtaaacagct ttccgtcgga
    16741 aaacgtgcct gcccctccct ctccaaaaca gtaattggaa tcctccatca ccgtgccgcg
    16801 gcgcaggatg ggagccaggt caaaccggcg ggaggaaacg tccttccccc gttccagaac
    16861 cacaggcttc acacccagtt ccaaacagcg gagcgcggcg aacatcccgg ccggtccgaa
    16921 tccaacgatc agcgccacgg gggaaccggg acggacggac ggataatccc gggagggaag
    16981 acgttccggc ggcagcgggc tatccacccc gaccagcagc cgaagctgga agagaatttt
    17041 tttctgccgg gagtcaatgg actccttcac cagccgcaaa tccctgatac gggaggggga
    17101 aatacccagt ttccgcgcca cggcagcgcg gcgcacgcgc tcgctgccgg ccttttccat
    17161 cggcagaatc acatctaccg ttattccttc ccctccgctc acagtccctc cttccccggc
    17221 cggggctgct gctgggtgat gcagtggatg gcgccgcctt ccagcaccag cttgcgggca
    17281 tccaccccta ttacctgttt tccgctgaaa cattcacgca aaatgcccag ggcgcgatcg
    17341 tcattccggg gctggttgaa tacgggaaca atgacggcct cattaacaat gaggaaattg
    17401 gcgtaactgg cagggagctg atccagccgc cagtcctccg catggagggg atccggcatc
    17461 ggcaggggaa tcacctccac cccggaaccg tccacgcatc tcaaatcctg aaggcgttca
    17521 ttattctccg ccagagcgcg gtaatgggga gaggaagagt ccttctccac gatggaaact
    17581 acggcatccc ggcacacaaa acgcaccatg tcgtcaatgt ggccatccgt atcatcccct
    17641 tcaatgccgg aaccgagcca gaaacggctc tgacgcccag catgcgcccc aactcctcct
    17701 caatcatggc ccggctccaa tccagattgc ggttggggtt cagcagcaca gactccgtcg
    17761 tcaccagcaa gccgtcccca ttgccctcaa tcgcccctcc ttccagaatc agggaagaac
    17821 tgcgcacggg aagcccaagc gccgccccaa tcagggcggg aacgccgttg tccaggtccc
    17881 acggggcaaa tttgccgccc cacgcattaa attgccaatc cgccagcatc agggaaccgt
    17941 ccttcacatc ctggacgaaa acggggccgt ggtcccggca ccatacgtcg ttggtcgggt
    18001 ggttgaacag gcggaattgc tcctcactca ctccggcggc cagcatcgcc tgtctgacgc
    18061 cgggatgaag gggagccgcg gcattcacgc atacaagggc atgcggagca atggcagcgg
    18121 ccacgctccc ataagtctgc tgcaactcgt ccagcccccc ttgccataaa tccctgcggt
    18181 ggggccagga cagccagaca gcatcctgag gctcccattc agcgggccag cgtacatcgg
    18241 gttccttgtt catggcggta tcgggcggtt caactgggct ggtcctgaaa ttcataaggc
    18301 tcttcaaaag ccttgcggaa gctcatgaag cccttgaaat catccaggga atcctccggc
    18361 tgtttggaaa aaacgtcttc ctcaatcagt aaaaaaacca tctcccccac attcttcccg
    18421 ttcctaagcc cccattcctt catcacggtg gaagccatgg gcccgaactg ggcgagggcg
    18481 taatcccgga acccctccaa cagttcctgc ccgctgacat gcctctgaga gccgttttcc
    18541 ctttcttcaa cgcgctgcat ggtaaaatcc agcgcatctt tcagaaaaga atacgcccgt
    18601 tccgcaaaac ggggatcctt ccgtacgata cggctcacgg catcttcaaa cgtaggggtg
    18661 gtcatagcgg ctcgccacta ccctatacgg ccccttgtga aaatcaagat ttttgggagc
    18721 gctccagctt cagccctccc ttggggacag catggggaaa atgaaggcag ccgccccctc
    18781 gctggaacgg aacgccggac gcggcagacg gcaaaattcc cgtccatgat atagagcctc
    18841 aaaaaacgca cgcgcccgca ttttgaatat catgcttctg catgagctac cctccggcaa
    18901 gcagcggttt ttacgttctg atgccgtcaa aaagccctgt aagcaggaag gaaaccttgc
    18961 cagcctatgg tctgtttgtt atgaacggaa tactttatgt tccgttcctc catcatgctt
    19021 ctggcaacca tgctgtgcgt ttcctgcgta tcgcaccggc ctatccagga cagcagctcc
    19081 ccgcccattg acgcggccaa tcccctggac ggcacccccg tggcgctggc gtggagctcc
    19141 ggaacgcaat taatgatggg cgtagacacg ggagcagtcc agacatccct gcttttctcc
    19201 ccggcagtgg aatccatcgg cgcacgcctg cgcggcaggg gcgccatgcg cacggccaac
    19261 gtgcccgttt ccctgaagga tgacggggaa cccatttccc ggaaacagga cgtagtaatg
    19321 gcagaccagg ccccgtatga cggtttgctg ggctgggaat gcatccggaa atatgtgtgg
    19381 aacatcaact atcccaaacg ctcccaccgt tttttcaata aacttccctc cagaataaaa
    19441 agctggaaca agctttccct gattcccggg tccgactatc cgcaaatcgc ggacaagcac
    19501 ggaaggcgca tcattctgga cacgggagcc ccccacgccg tttacatctc caaaaaacgc
    19561 tggaatgcca ttaagcaggc ctacccggat gcgttcgtca gcgtctattc cggctacagc
    19621 cccgccgcag gcggctttta cgcccacgaa tgcatgcatg taagctcctt ccagctcggt
    19681 ccactggaat taaaaaatat cctgctctgt gaaagcttcg ccaacccgga agtgatgggc
    19741 atccccgatg acatcgacat catcctgggc tacggcgctc tggccgcacg ccagttctgg
    19801 ctggacggcc cggggaacgc cctttatttc agctccacca gccaccggat gcccgccccc
    19861 gcctccttca acctgatggg aggcaccttt atccaggaca gcaacgggaa cggccccatg
    19921 aaagcttacg tggcagagtg gtcgcccgca tgggacgccg gcctcagaac gggagatgtt
    19981 cttatttcca tcaatggaag aaagaatccc tatccggacc tcgtagaata tgttaccacc
    20041 cagcgggggg ctcaggccag cgtggtggtc cagcgcagga acaggctggt gcgcatccaa
    20101 tgggaagttc cggccgcgcc ccctgccggg gattattacc ccacgcccca ggccattacg
    20161 gaacaggaat tcgaaaacca cgtcaggcag caggaaaaaa aagaacagac ccagccctcc
    20221 gcagacggcc agcagcctcc ggctacggcc ggagaaactc cggatgaagc ctctcccgca
    20281 gctgacggga aaacggacaa ggcctccgct gcctgagccg ggccagcaac cagccatctc
    20341 tctacaccgg catgaacaac gaacaacatc agaataccca aacttccgcc gccctcgcca
    20401 ttccctcccc tttccagaaa aaaacctgct ggcatgccct gacaggcgcc tccattctgg
    20461 tgatgctggg cattgcggcc ttcgtcattt ttgaagtggt ggaacttctg gggtttctgg
    20521 aaccggtgct cctgcccatc ctgattgccg ccgtcgtggc gtatttgctg gaacccatcg
    20581 tttcctggct ggtgcgtctc aaattctccc gtccatgggc agtagtcacg gtcatgtttg
    20641 cggctctggc cgttctggta ggctttggag ccacgattct ccctcccctg atcaggcaga
    20701 cggatgaact gatcgacaac cggatggagc tatgggacaa aacctccgaa ctgattgact
    20761 ccaccattga aatccccttc atttcccgca ccattgacag tgtttacagc accagcctgc
    20821 gggaactgaa tgccagccat tatacggaag cggaagtcca tgacctgaga aacgcccgga
    20881 ccgcccggga aaagctggga gcctacatga ccatcaattc ctccttttac caggacaagc
    20941 tgatgagctg gctcacttcc gggggacggg ccctttacag caccatagga atcatggtca
    21001 gcatcctgat cacgcccatt ttcgcttttt attttctgct ggaggcggat aaaatcaaag
    21061 agaaatggcc cagcatcctg cccctgaaag tctccaaatt cagaaaagac gtggtggaca
    21121 ccatggagga aatcaacggc tacctgattt ccttcttccg cggccaaatg ctggtaagca
    21181 tcattgaagg cattctgatt gccatctgcc tgaagctgat cggcctgccg tacgccatta
    21241 ccattggcgc cgcggtctgc gtgctgggca tcattcccta cctgggtatc atcaccgcct
    21301 ttatccctgc ggtgttgctg gcctggttca catgggggga tttccagcac gtgctgattg
    21361 tttcgggcat cttcctggcc gtcaaccaat ttgacggatg gatcatccag ccgaaaatcg
    21421 tgggggattc cgtggaactc cacccgctca cggtcatgtt ttccgtgctg atctggacac
    21481 tcatcctggg cggcttaatc ggcgccctgc tggctgtccc cctgacagcc gccatcaagg
    21541 tcctttacaa gcggtacatc tggcagaatg ccagcatgcg ccccatgacg gaacccgtgc
    21601 ttccgccgga acatcccggc gaacagcctc cggacacccc cgaagattcg gcccacgctt
    21661 aaacagcggc atccttccga ccgccctttc cggaaaatta taatcacaca cacctgacaa
    21721 catgaataaa tccgcattac acctgatgct tgccctggcg gcggcagcgg cctgcccggc
    21781 cgcagtcacg ccaacggtca agccccccaa ggccatcgtc atgatttacg ctgacgacct
    21841 cggctatgga gacgtaggct gctatggagc caagggaatt cccaccccct ccatcgacaa
    21901 gcttgccgag cagggctgcc gcttcacgga cgcctattcc accacatccg tctgcacccc
    21961 ttcccgctat gccctgttca caggagaata tccgtggcgc aaggaaggca cgggcatcct
    22021 gccgggagac gccgccctca ttatcgatac caagaagccc accctgccca agatgctcca
    22081 atcccacggc tacaaaacct acatgatagg caaatggcac ctgggcctgg gggaaaaggg
    22141 gaagaaaatt gactggaaca aacatatctc ccccagcccg aacgaaatcg gatttgacga
    22201 gagctttatc ttcgccgcta cgggcgaccg cgttccctgt gtgattctgg aaaacggcaa
    22261 tgtccgcaac ctggacccga acgaccccat tgaagtatcc tacaggcaca acttccccgg
    22321 gcttcccaat ggcaaggaca ataaggacca gctcaaactc atgtggagcc acggacacaa
    22381 ccaggccatt atcaacggaa tcggacgcat cggattcatg aagggcggca aaagcgcctt
    22441 gtggaaggat gaggaaaacg ccgatgtcat tacggataag gccattgaat acattcaaaa
    22501 gagcgccaaa gccaaggaac cgtttttcct gatgttcgcc acgcatgaca tccacgtgcc
    22561 gcgctgcccg gaaaaacgct ttgtgggcaa gagccagcac ggcgtgcgcg gtgacgtgac
    22621 cgtggaactg gatgactgtg tgcgccgcat tacagaggct ctgcaacagg ccggtctgga
    22681 aaaagacgcc ctggtgatct tctccagcga caacggcccc gtgctggatg acggctacag
    22741 ggatttcgcc gtccgggaca acgccaccca ttcccccgct ggccccttcc gggcaggcaa
    22801 atacagcatt ctggaaggag gttcccgcat tccgttcatc gtcaaatggc ccggcgtgat
    22861 caaccccgga accacgagca aagccctgct caaccagatg gatctgggag cctccctgga
    22921 acaactgctg gcccccggca aggccaattc cttccgtgac tctgaaaacg tgctgcccgc
    22981 ccttctgggc aaatccgcca aggggcgtga ctaccatgtc atcaacagca ccggcaaggc
    23041 gttggcgatt cgccacggca aatggaaatt cattcccgcc ggcgtggcca ttcgcgacgg
    23101 catcaacgga gcctccgcaa aaatgagcaa gtccccggaa ggaggaagcc tctttgacct
    23161 ggaaaaagac ccgaaggaac ttgacaacgt agcctcccag catccggaca tttgcgaaca
    23221 gatgaaagcc aagcttgagg aaatccgcca gaggcccgaa accaaggctg accaggagga
    23281 cctgcttccc ttggacgact aacatccatt cgggccagat catgagagaa tccgccacat
    23341 gctcccaatg cggccacggg tacacctacc cggccggact ggacgccaaa accggcatgc
    23401 gctgcccggc gtgcggcgtt ttgattcatg ttacaacggg gcttcccttc ggggaagcct
    23461 cctcttcgcc atccggccgc aaaccggaaa acgccatgaa aacgcaggcc gccctgcctc
    23521 ccgtcaggaa aaagcggccg gctttctccg gacgcgatac tgacgaacgc ggcatccggc
    23581 aggaaaacct gccgcagctg ccagccgcag catccggccg ccggattccc gctacatcat
    23641 ggctggcatg ggggctgtcc ctgatagcgt tgattctggc ctttattttc ccctcctacg
    23701 gaaacggttc cacagatccg ctgtacatgc ttcagtgcct gccctccatg gtattcggct
    23761 gcactgcggc catccttttc atccagggcc tggcggcttt gcgccgctaa aaaacaaagt
    23821 ctgcggcccc atccggggcc catgcggcag ccccgtccca aaaacggccg gaatgcctgg
    23881 tttccatgcc cctttccgcg gaaccgggct tttaggcttg cctgtcaaat gaacagattt
    23941 taagttacgt cccggtaaac gttatggaac gggaggcaga ggcgaaggat aacctgtgga
    24001 gggccagact ggatgccgtc ctgatgcaca ggatatgggg ttcccttatc ttcctgggca
    24061 tcgtttatat tattttcttc ctgagcttcg ctatcgggga tcctctggtc aggctcatcc
    24121 agacgggcac ccagctgttc agcgtctggg ccagccgcat gctggaaccg tggccccgcc
    24181 tgcaatccct gctgggggaa ggcgtggtag gaggcgtggg cggcgtactg gcctttctgc
    24241 ccaacgtggt tctcctcttc ggggccatta ccgtcctgga aaacagcgga tacatgatga
    24301 gggtttcccg cctgatgagc cgcatcatga aaatcatggg gttgaacggc agcagctttg
    24361 cccccctcct gctgggattc ggctgttcgg ttcccgccat cctgtccacc aggagaattg
    24421 aaacgcgcag cgaccgcctg gtcaccatcg ccgtgctgcc catgatgagc tgtgcgggcc
    24481 gcctgccgat ctacatgatg ttcgtctccg ccctgtttcc ttcccgcctg caggcccccg
    24541 tgctgttcgg gatttatgcc agcggcgtcc tgctggcgct gtgctgcgcc cggcttctga
    24601 aaaacacgct tttcaaggcg ccccagaggg attccctcca ccacatgaag cgcctgcgcc
    24661 tcccctccct gcggaaggtg ggcatcctga tgtggtcgcg ggccttcatg tacgtccgga
    24721 aagcaggcac tttcattctt ggggcctcca tcatcctgtg gtttctgaac acctacccca
    24781 ggccggaaga agacacggcg ccgacaaacg cagcggccat ggaacattcc tacgccggac
    24841 aaataggcca ttggatggaa cccgtcacgc aagtggccgg atttgactgg aaaatcaatt
    24901 ccgccctggt aggagccttt gccgccaagg aaattttcgt cacccagatg ggtatccttt
    24961 atgcggtgga agacggaaat tcggccccgg cggcccagca gacattgaac gcccgtctta
    25021 aagccagcta caccccactc cagggcatca gcatcatgat attctgcctg atcgccctgc
    25081 cctgcatcgg caccgtcacc gtcgccaaga gggaagcggg cacctggtgg ttcgctctgg
    25141 ctcaattcgg cggattgacc ctcctgggtt ttgcaacggc tacgctgatc taccaggcag
    25201 ggctccttct gtaaaggcca gccataaaga aaccggaagc aggaaatatc ccccggaaga
    25261 agaaaaaatg aacctcgtga aaaaagaggc gtttacgatg aacttccgca agttctcctg
    25321 ctctacggtc aaatgatcac aacatcgcca tcatccggcc acgaaagccg cagcataaca
    25381 ccatgcatgg ccgtgcaagc ctcccctccg caacaatccg gaatcagtat gcaccgctga
    25441 agcctttcct gttttttcaa gccgtccggg tatccttttc cgtttgcaga gggggggaca
    25501 acctgcggca aaaaagatca tgcagccatt tccggaaaat caatgccctt ttgacaggga
    25561 gtaatcaaaa aaggggcacc aattatccat tcggtaatct ttgccctgga tatgggcctt
    25621 aacctcatct ccctgggaat ccctcagaca gccccgcgcc cccgtccgcg gaccggaagg
    25681 acggtgttca ttaatcgtca tcccatagcg tagacgccct ttccccgggt ccttctccgt
    25741 caacagctgt accgcatttt tccccaccag ccggacagac ttgatggcaa tatcctctcc
    25801 ctcagccccc acaagggaaa aaccataatt gccaggatcg tcaaccgtct tcgtgtccag
    25861 aaccaaaggg ggacagggag cataaaatgc cacttccacc gtccaaccct ttccgaactt
    25921 tctgatttta tggttcaggg gatgcacggg tttccagtcc gccccatcca acaggacttt
    25981 cttcatgaca tagcccacat actccccata aaggcgggac atgggcgccg tcaaatggac
    26041 tccgtcgcta tactcgaaat ggtacatcgg ggtcgtcatc acataccggc tgtccttctc
    26101 caaagccata tccagctgtt ccagggcaat agccggataa tccgtaggat taaactgata
    26161 ataattcaaa tgggaaccaa cctgatagcc gaaacagacc acatcgtttt tctgcccggt
    26221 cacggccctg gcgtcatgat ttatgtccct gatcagcttt tccatttttt cccgatacca
    26281 ttccctcgtc tttctgtcct ggtgatccgt ctctccctgg gtccagataa acgcaggcat
    26341 ggaaagggtc ttcccttccc ggtcagccag tattttggcg gccttcaaat catccaaaat
    26401 gcggttgtag gctccagttc ccctgcccag ggaaccaatg ctcgccccac ccactcccgt
    26461 cgcagaacaa atcaaccgga tacgagaagc cgtaacggga gaataacgct gcatcaggcg
    26521 gaacaaacga caggcagccc ctgatacggg agtttcaccg cgcggcccgt tcactccttc
    26581 ctccagagga accagcatct tgcggtcact gccgttctca tacgctctca cccctcccct
    26641 gaacatatac aagccccctt cctcctgctt ttccgttaca acgggcttag ccgtccagcc
    26701 cacagaaagg gattgaccgt aagaagttaa ataattccaa tccgcccact ctgttttccg
    26761 ggaggcacag attgatgccg aagccgccgc agccatgtgg aaagcaagca aaattaaggt
    26821 aacaggaata ttcattgata attcaacgga ttctctccgt gaacatacgt tagccgtccc
    26881 ctctttttat caattcctga acacaaagct atccatccat tgctgcagta ccattccgcc
    26941 aggaaatacg gaattgccgt tccagccggg tccacgccat gtttcgggac catgctgtac
    27001 cggcaggaaa acgtattcaa cataccgacg ggatttttca tcctccaaaa gaaaggcggc
    27061 agaaacattc cccgtattgt ataaacaaca tgaatcattt cccgtctatc aacgccatat
    27121 ccgcctccat ctgccttctg ggatgcggac tcgccctgcc tgttttctcc caacagccgg
    27181 aaaaaggaaa acccgatgcc gcccacattt ccaaaacaaa agctgactgg tggagccnnn
    27241 nnnnnnncca gaggcacgcc ctgttaaagc agaccctggc ggaaacacca tgccaactgc
    27301 tgtttatcgg cgattccatc acccaccgct gggaaaccga cggcaaaaaa atatggtccc
    27361 aatatttttc cccctatgct cctgtcaatt tcgggatagg aggcgacagg acggaacatg
    27421 tattgtggcg cattgacgat tctgccctta aaacgccgca ttccccccaa gtatgcgtta
    27481 tcatggtggg caccaataac acagggcaat acaaaggcag gcagaccccg caggaaacgg
    27541 cggagggtat ccgggaaata gcttcccggg tccatcggct ccaccctgcc actgaaatca
    27601 ttcttctgca catctttccc cgggggaaaa cggcggaaga ccccctgcgc atccaaaatg
    27661 aaatgatcaa cagggagctg gacaaaacaa atatgccgag agtccatgtc gtcaacatta
    27721 attccgcatt cctggacaag gacggcactt tcctgccggg aattaccggg gaccccgtcc
    27781 accttacaga aaaaggctac cgtctctggg cggacgcact gctgccggaa atcaaaaaat
    27841 acatgaagta agtccgctac ccctttccgt ccggctcaac ggacaatcca gcctcatctg
    27901 cgcagaagcg gcaagccaat catggttgtc actcccgcac ataaaaaaac cggagggatt
    27961 attttccctc cggttttcga tacggccgtt aaaaccgttt aaattgtctt aatcacggaa
    28021 gagtcgtcat catccctgtc ctcggcagaa gcggccttcc tggtgggaac aggcgtcttc
    28081 ctgacctttt ccagagcctt gtccaccgcc gcaatcacct tgtcttcccc ggtcttctga
    28141 ctggacaact cattataaat cttttccttg gcctcataat cggccttggc taccgcatcg
    28201 ccgggattgg ccttgaactt gtcctccgcc ttgaacaagg cttcctccgc agcctggaac
    28261 tttggttcca ccgcgtttct ctggtcttcc gccgtctgct tgatgcggcc cagcaggcga
    28321 atcacatacg ggcggttgct gagcctcttg aatgcggaaa ccagaaactg gcggcgttcg
    28381 ctggttttgg catccgcaat aagggggtca aacaacttga gcgtggcgtt gtcatcccgg
    28441 tcacggtcca cggagagtac gcggagaatg gaatcatggg cctgggaacg cacgcgttca
    28501 tgcagggatt cgtctttcca ggccttgaac aggtacggaa cggcgtcatc cgtattccag
    28561 tccccccagg cggtaatgat ggcaagcgcc tttttgggat cctccttata gctcttttcc
    28621 atcatgtcca gcaccttggg atcaccggtg cgggccagca gcttgtagag cattttgacg
    28681 ttttcctccg gcatgttttt ctgatactgg agcagtttgg aggaaagcgc cttgcgcttg
    28741 gctggaggag cttcgtcaat caggtagcgc gccgtacgga aagcggagga aatcaaatcc
    28801 ggggaagcat ccttcttctg gaggatcttc atcacgtcgt caaggtcgtc cagcttcatg
    28861 gcaaaacgca tgtacttgag caccacggcc tgcttgtcct ggatcttctt attcttgctt
    28921 tcgctgatgg aagcgtacag gtctttcagc atgtcgcgca tggcggggct ggaagacttg
    28981 gccaaaaggg tcaccatcat ggaatatttt tccttactgt acctgccgac attttttttc
    29041 atgtcttcca ccacctggcg ggcaatgttg tcatccatct gggccatgat ggaaaccagg
    29101 tgggcggcgc cgggccagtt ttccctgttg cccttgactc ctctcacatt ggcgagcagc
    29161 agagcggcat cctccgcgtc gggaacgact ttcacatccg gaacacggct gagattcttg
    29221 gagtcaaaca gacggctgcg gcccagtttc ccgccttcca cgaccagcgc attcaggcgg
    29281 gcgttctcac cttccacctt ttctttcttg ctgctggtgc tccatgcata aatccctacg
    29341 ccggcagcga tgacaaccag ggcgcatacc ccataaagga caccccgcag cagggatttg
    29401 gatttatcca cggaagcggc catatcccgg gcggattccg ttccggcggg aggctgaata
    29461 ccgccgtcgc ccgccggagg agcgccagag gcaggggaag ctcccgcggc agccacaggc
    29521 tggggaaccg cagcgggagc catggaagaa gctggtgctg cccccacggg ggattttaat
    29581 actttcagct tgggaacggc gctttccgga gcggcggccg gggatttctc cgccgcagcg
    29641 ggagccgcgt ccgtcttgaa aactttcaat ttgggagccc ccaccgcagg agcaggctcc
    29701 ggaacagacg gttccggggt ctgcacagcc gcaggcgcta cccctgcggc ctgggccagt
    29761 gcagcggcct gagcctccgc cagggctttc tgggcctccg ctaccttggc aagggcatcc
    29821 tgaatctggg catccacgga gggctcgggc tgcggagcgg aagcggcttc ctttttctcc
    29881 agctccgccg caaggcgtgc tctttcctct tcggcagcta cacgggcggc ctccttttct
    29941 gcggcaatgc gcgcggcttc ggcagcctcc tgctccgcct tgattcttgc ggcttcctgc
    30001 tcggcagcta ttctcgccgc ctccttttcg gcagcaatac gggcagcttc ctgctcggca
    30061 gccgcctttt ccgcagcaat cctgtccgcc tccgcctgtt ccgctgcgcg gcgggcggct
    30121 tcctcctgct cctgagccag cttggcctgc aactcaggag tgagaaaacg cggcttgaca
    30181 acctccacgc cttccgcggc gggagaaaca aactttacct tgggtgcggc cgcaggggct
    30241 gccgttacca aacggcgtcg caatttggga ggttccggcg aagaaggggt tcctgtctga
    30301 tcggtcatcg tgataaaatg gctgggaaat tggagtgatt ttatacgatt ccgcggtccc
    30361 ctgcaacctc aaaataccgt ctggaaaacg ggcggggaac agccgccgtg cgtggttcaa
    30421 caggcagatt gcgaaaaaag gcttcacccg agatataaca tggaaaagcg tttaaatatc
    30481 ccgcgtattc tcccgcaaaa tcataaaaaa cacaatatgt taacaacaac aatcatgaat
    30541 aaagtaaagc gcccgtaata gaacttcaag aatacgtacc agtcatttaa gaatcctctt
    30601 aaaaaatgtt ataacacata tacttttttc ttgccagacc aaggatgatt agttaaataa
    30661 ataaccactc gtcaacatca tgaactccaa cgacagtacc aaaaataaga ggcgctacac
    30721 gcctgaagaa aaacaatccg ttcttgattt cattgctgaa cggaaaaccc aaaacaaaag
    30781 gggtgcccag aaagaagccg ccgaacgctt cggcatcagc acggtgacca tctcaagctg
    30841 gatgaaggca tccaaggcca agcgcggccg caagcccggc accaaaaata caactgctcc
    30901 caaaacacag cctctcgcag ccgcccaacc cgccgacctg cgccgcctgg cggccgttct
    30961 ggaagaaatc gccgaccttg aagcccagac agccaaactc gacaccctgc gcactgaagc
    31021 ggaggaactc aagaacaggc tgactgccaa gcaggcctga ttgcttttat tccttccttt
    31081 tccggaaaac cggcttcccc tgacaaggga agccggttat tttctttacg gagatcgcgc
    31141 ttgccccccc ggaacaattc ctttaatatc cgccgtcgtg actcaacccg cccacattgc
    31201 tcccgcgagt cagtctgtgg aaggcatgta tgccgactac tttctggatt acgcctcgta
    31261 tgtcattctg gaacgggccg tacccaagat caatgacggg tttaaacctg tgcagcgccg
    31321 tattctgcac gccatggacc gcctggacga cgggcgttat aataaagtgg ctaatatcgt
    31381 gggagacacg atgaaattcc atccgcacgg cgaccgttcc attgcagacg cgctggtggg
    31441 gctgggacaa aaagggctgc ttatcgacac gcaggggaac tggggcaata ttctgacagg
    31501 cgaccccgca gcggcttccc gctatattga agcccgcttc acctcctttg cgcgtgacgt
    31561 agtattcagc cccaaagtca cggaatggca gctctcctat gacggcagga acaaggaacc
    31621 ggtcagcctt cccgtcaaat tcccccttct tctcgctcag ggagcggaag gcatcgccgt
    31681 ggggctttcc agtaaaattc ttccccacaa cttcaatgag ttgattgaag cctccatcgc
    31741 ctacttacgc ggccagccct tccagcttct gccggacttt ccgaccggag gcgtgatgga
    31801 tgccaccaac taccgtgacg gagaacgagg aacgggccgc gtccgcatca gggcgcgcat
    31861 cctcacggaa tcgaagaaac ttctccgcat cacggaaatc ccgttcggcg tcaccacgga
    31921 aatcttaatt gattccattg tctccgctgc ggaaaaggga aaaattaaaa tcgcccgcat
    31981 tgaggacaat acggcccagc atgtggacat cctggtccat ctcccggccg gagcggatcc
    32041 ggagcagacg aggaaagccc tgttcgcctt ctccgcttgt gaagtcagca tctccccgaa
    32101 tgcctgcgtc attgtggagg aaaaacccag gttcatgtgt gtcagcgaca tcctgcgcta
    32161 caatacggac tccaccaagg aaatcctgcg ccaggagcag gaaatccgac tcaaggaact
    32221 gaacgaggcg tggcaccagg caagcctgga aaaaatattc attgaaaacc gcatctacct
    32281 ctccatagaa gattccgaaa cgtgggaaga agtgctcggc accattgacc gggaattgca
    32341 gccgtttgca tcccggctgc gcgcccccat taccagggac gacctggtaa ggctgacgga
    32401 aatcaaaatc aagcggattt ccaaatttga cgctttcaag gcggaccagc acatccgcca
    32461 gcttgaagag gacatcgaac aaacgcagaa gaacctcaac cagcttacca aattcactat
    32521 ccgctggttt gaagccctgc gtaaaaaata cggcgccgcc tatccgcgga aaacggaaat
    32581 ttcctccttc ggctccgtaa accgcgcgca ggtggctgtt gccaatgaaa cattgtatat
    32641 tgatgacgaa ggctttgccg gttacggcgt caagaaggga aaccctgtct gcaaatgctc
    32701 cacgctggat gacgtgttga tcattgacaa tgcaggcgtg ctcaaaatcg tgcggattca
    32761 ggacaaattt ttcgctggta aaaaccccct ttatatttcc gtcatcaaaa aaggggacga
    32821 ccccgtgttc aacctgattt accgggacgg aaaagacggc cccgtgtacg ccaagcgttt
    32881 ccgcatagga gggttcacca gggacaagga atatccgctt acccgaggcg caaagggaac
    32941 gcgcatcttc cacttctccg tgcatgaaac ggaggaaaac agttcccaga taagcgtaaa
    33001 cgtttatctg aaagccgttc tgaaactccg taacctgatc aggcccttcc acttcgcgga
    33061 cctgagaatc aaaaaccgcg gtgcccaagg aaatatcatt accaggcatc ctgtggaacg
    33121 cgtctcccgc atcatgcctc cggccaagtc cggaaatgag gaaacggaag agccaacaac
    33181 cgctccttcc gcaacagcgg aacggacgga aggctcaccc gccccctccg cggaaacgct
    33241 gcatcctgaa acagccccac atttggagga accgcctgcc gatccgccgc tggaacaggg
    33301 ctccctgttt gactcctgaa acgttttcaa tctcttaaaa tggaaatatc catgcctgac
    33361 tatccatttt cggcttgcca caacgggcca tcttctgtag atatggacca cctcattacc
    33421 atgccgcttt agctcagtgg tagagcaccc gccttgtaag cggacggtcg tcagttcaaa
    33481 tctgacaagc ggctccatca taacccgctc aaggtcaatc cttgagcggg ttgcttttat
    33541 gaggcgtgcg ccattactcg ttcccatgaa tttccggcgt gccgcttttg aacattcatc
    33601 atccattcat atctccggcg gaatgcggca gccgcaggaa gcaaagcggg acgcaacatc
    33661 tggcaagagg ccattccacc cacaggcatc cccgtcaact tccggcagcg aagggccatt
    33721 cccggagcgg gaagaaggtt ccgttggcga taacgccgcc tcagcaagcc gtttcccccc
    33781 ttgaatacgc ctccattgcc atttcgacca agggaaccat gattcaagcc aatggatact
    33841 acagaaaaaa agatggccgc ggctatcctc cggtttgaag acagccgcgt taccgggccg
    33901 gattccctgc gcgtttctcg ccttcctgcc gccgacaagg gcggcaagtg ggagatttgc
    33961 ggcatttgcg acggtattga ac
//
LOCUS       DAOMSU010000002        32877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_21_length_32877_cov_3.013771, whole genome shotgun sequence.
ACCESSION   DAOMSU010000002 DAOMSU010000000
VERSION     DAOMSU010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 32877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32877
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21_length_32877_cov_3.013771"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            415..2412
                     /locus_tag="PK077_00155"
     CDS             415..2412
                     /locus_tag="PK077_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRN22743.1"
                     /translation="MKFIIPALALSAFLPAAFSQEQIIPKPAEITLFTGSPARLTPDS
                     LIITETQDKAFLDQAGQLQQMLSAGTGLPLPLKPAGQASKKAACIVIKKDPALAARGE
                     EAYSIQSSPSEIILSAADARGIFYAGQSLVQMMPSVFHDRTADKSAVRWNISETPFRI
                     TDYPRFSWRALMIDEARHFFGEKTIKQIIDQMALLKMNILHWHLTDDTGWRIEIKKYP
                     RLTSIGSKRRESEIGTWNSGKSDGTPHEGFYTQEQIRDIVQYAARRNITIVPEIEMPG
                     HASAAAVAYPFLSLKTPGEVPTTFIVNTAFDPTSEKTYAFLSDVLDEITALFPGRIIH
                     IGGDEVRYDKQWKGVPEIEEFMKKNGMRSYADVQMHFTNRMSGIIAQKGRSMMGWNEI
                     YGHDVNGDGGGKAGAKLDTNAVIQFWKGNTGLAKNAIRDGHDVINSLHTSTYLDYSYG
                     SIPLQKAYGFEPVFPGLEEQYHSRVKGLGAQVWTEWISTPERLHYQAFPRACAFAEVG
                     WTPAGKKDFPDFKKRLKAYSERMDLMGIKFARNVISQIDKSDFFNTPRIGTWTPATLT
                     REEHSFDVTKLVKASGKHTVTLLYDKGAHAIEIESVALYENSREISRDAHAGRSGAYK
                     ENIQYILNAPEPRQGATYTVKAKFKGDGGRDSHGTVYFETP"
     gene            complement(2746..3084)
                     /locus_tag="PK077_00160"
     CDS             complement(2746..3084)
                     /locus_tag="PK077_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22744.1"
                     /translation="MDTTIEMSTTLSKNPQCGREWCYKGISHIVVKYSGPISDPIDGG
                     TIYFEPYYNHHIWFDKKKYGEWGILYSKVYIITRIKNANIEDVRDYGISGEDGSIFFQ
                     YVEKVVDDNM"
     gene            complement(3259..>4029)
                     /locus_tag="PK077_00165"
     CDS             complement(3259..>4029)
                     /locus_tag="PK077_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22745.1"
                     /translation="IGEQNNWGKELAASMEQWYSYPVEARTRVSRFAGSGVYKRFMSR
                     KGGIYLFRICRNGSFNTILNGVFVNEKMPWEIRMPEELPYYVSGALAGIVPTPERVSG
                     ATLGQREKAVCKPLYELQYTRKYLTPAACSLQNRYILSLWRQARERRAEGGCLADCLQ
                     WEARVSDDRVRASFDETMKRSWDQSQIYYIGNRSRDFMPNAPGTVPFSLSELRLMAKL
                     RIDWRQYRDDAKTPPEKTVQEMKEFLNKELLKQQQSRK"
     assembly_gap    4030..4047
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4048..5484)
                     /locus_tag="PK077_00170"
     CDS             complement(<4048..5484)
                     /locus_tag="PK077_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22746.1"
                     /translation="MYDRSNALNGEHVYALAISPVSGEVAVATSGGVSIYDPAGDSWK
                     TLDRSTGLVEDQAASAGFDTRGNLWLAYACGGVSCSSRQSKYMQWKNVQAPWYWDNRQ
                     FARQPYQPYGDGLPSNICNVLTCTDKGQVLVGTCSGLAYSNGISSWRYMRGRDYAQKN
                     NHLYGSAARKTAVPGESNSRMLSEDFISSIIQQEKEIFIGYRMQGVDVLDAEKMTVNQ
                     RIRKGLENVDVPSLLALQDGSVWAATYGRGFVRLKKGTLAYKLDREQLDNEVPFPRPA
                     QMEEPSVVLRRLERLGSDAHAGKNIVFRGEDWTTKGDWCGKYGHTRATLCATNAPMSN
                     SEFKAKPIKFRTLSSVPGHPGYKGALSPYWIQGLMGLNRKKGDALRWWVHSIKENDNR
                     NVLFDPTDSVRTEAEWDDHGEVYPGFVDGPDIWVAVEVPEGVHEIALYFYNPNGYLTN
                     ESRRDYVVEARRHPSTAPLVFQFNIEGDL"
     assembly_gap    5507..5516
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5517..5794)
                     /locus_tag="PK077_00175"
     CDS             complement(<5517..5794)
                     /locus_tag="PK077_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22747.1"
                     /translation="MLIFLLLGAWAAGKVVTVQIPEQLPECPVRFIRGTLVAGDGSIW
                     AVGEKESIYRLQIGDRTYEKSWLNMDYYSGFPKGKNFTCIAEDRQGRI"
     assembly_gap    6913..6922
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6998..8398)
                     /locus_tag="PK077_00180"
     CDS             complement(6998..8398)
                     /locus_tag="PK077_00180"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004351 - glutamate decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006540 - glutamate decarboxylation to
                     succinate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HRN22748.1"
                     /translation="MFDPNQKPDTARAENSIFGSPEADNLLPKDEFPDYAMRAEDAFQ
                     LVSDELMLDGNARQNLATFCQTWDEKQVRMLMNLSINKNMIDKDEYPQTAAIEMRCAA
                     IIANLWNASQNEKPIGCSTIGSSEACMLGGMAALWRWRARRKAAGKPIDKPNLVCGPV
                     QICWHKFCRYWDIEMREIPMAPGKWKMDVDRMLEQIDENTIVVVPTFGVTYTGAYELP
                     AEIAKALDDYENKTGISVDIHVDGASGGFLAPFCAPDLVFDFRIPRVKSISASGHKYG
                     LAPLGVGWVVWRDISELPEGLIFNVNYLGGEIPNFAINFSRPAGQIICQYYDFIRLGK
                     EGYTNIHTQAYEVAKYISDELSKLGPYEFICTGDPEKGIPAVCFFIKDGARANYTLFD
                     LSDKLRTRGWQVPAFTLPANCQDTVVMRVMVRQGFSKDLADLFLEDYKRMLSFFEKHP
                     VSSPMTAEEGTAFHHS"
     gene            complement(9051..9614)
                     /locus_tag="PK077_00185"
     CDS             complement(9051..9614)
                     /locus_tag="PK077_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN22749.1"
                     /translation="MPGGKLSLPESERLVLREWREKDWPFFAAMNANPQVMRFFPGVM
                     TDKDSLSMWNRMREELDERGYGLYAVELKHSGSLIGLLGFHWADFEADFTPCVEIGWR
                     LVPEAWGYGYATEGARACLKHGFARFGFDRVYSFTACVNFPSQAVMQRVGMRKIAEFN
                     HPKVAPDSILYPHVLYVKDTFRKIERE"
     gene            complement(9619..13143)
                     /gene="dnaE"
                     /locus_tag="PK077_00190"
     CDS             complement(9619..13143)
                     /gene="dnaE"
                     /locus_tag="PK077_00190"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRN22750.1"
                     /translation="MPGSFVHLHNHTEYSLLDGAVHIKDLIAECSRMGMPAVAVTDHG
                     NLFGSIELVMETNSLNKSVAAWNKEHPEGPQKQEVKPIFGCEVYLSPTPISVKKQLPG
                     RRKYTHLLLLAENETGWQNLVKLVSRSHLEGFYYKPRVDMETLREFHEGLICCTACIA
                     GPLNEWLLNDQPEKAEEALLALKDIFGDDNIFVELQDHGMEEQKKCLPELVRIARKTN
                     TPIVATNDVHFLRKEDHDMHDVLICIGTNEKLASPKRMRYSTEVYLKSPEEMREVFKD
                     YPEAVENTLKIAERCNVTIKLDSTSTEKYPEFGTPDGSSREEYLRKVCYKGLEERYGK
                     ERVAADKELRDRLDYELGIINQLKFPSYFLITADFINWAKDHDIPVGPGRGSAAGSLV
                     AYAMKITNIDPLRFGLIFERFLNPERVSPPDIDIDFCQTRRPEVIDYVRQKYGERAVS
                     HIITYGTMGAKSVLRDVARVMDMSYADGDRISKLIETGPKVTLQSSYKSNEELRELIA
                     SDEGYAELWGYATRLEGLIRNVGVHAAGVVIGDRPLDEHVALTRDDLSDPHAAVVAQC
                     DMSAIYEVGLLKMDFLGLKTLTVMHDAEQYARWRVPEFKLDSVPLDDRETLDLLNRGD
                     TMGVFQLESGGMVDTCRRYGIQKIEDIIDLLALYRPGAMQFMDEMIEVKKGLRQVEYE
                     HPLLEQVSGETYGVMIYQEQVQAAAKLLAGYTLGGADLLRRAMGKKDPAKMAKEKAHF
                     VEGCWKTNQIDEKTATAIFDKIEKFAGYGFNKSHSACYGHISYWTAYLKAHFPVEFLC
                     GLMSNEANNDKIGVFIQEANRMGIEILPPDVNKSMLKFTPEKMPSGKLAVRYGLAAIK
                     NVGEGAMQILLEEREKNGEFSSMEDICNRLDSKSVNKKILESLIRAGALDWTLEPRCV
                     LFERVDLALSGASQVHKDKALGQGSLFDMTFEAPRVKDEPAVPEWSKEQRMGDEKDLL
                     GAYFCGHPLDSMRGVIDAEKYTRIGLIDTLEPHELKQKHQIAGMVRSVTPKISKAGRK
                     FAILTLEDFTGSIEVLLWSDVYEKAQEMEGGLEPGVFVSMRANIREDDRTSSKSVAAQ
                     SIDPLGGKKRKSKSGDQGPLNIIVSPLRHTRKNLEQIRDILKRYPGRSKVNLTIKDSL
                     GHSQILELDERFRVNRCPELETELSMYN"
     gene            complement(13176..14222)
                     /locus_tag="PK077_00195"
     CDS             complement(13176..14222)
                     /locus_tag="PK077_00195"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HRN22751.1"
                     /translation="MSPALYSAAKSVLFQMNPETAHKVTLWGLRLAEKMRVLPLVMGE
                     VPSDPVEILGMKFLNRVGLAAGMDKEADTVSAFGQAGFGFVEVGTLTPRPQPGNEKPR
                     LFRLIPQKAIINRMGFNNEGIAAGVENIRSATRFHGVLGVNIGKNKITPNEDAAQDYL
                     TCLRAAWPVADYIAINFSSPNTPGLRDLQAAEPAARLLASLKSEQSNLAAETGRHVPI
                     FMKVAPDVTDEHIAELSRVFLDEGLDGLIATNTTLSRVGVEANPRHEEAGGLSGAPLT
                     ERSTEVIGAFASELKGRIPIIGVGGIMNGADAVAKIKAGASLVQLYTGFVYRGPDLIR
                     ECVEAMKAECPVHR"
     gene            14310..14954
                     /gene="thiE"
                     /locus_tag="PK077_00200"
     CDS             14310..14954
                     /gene="thiE"
                     /locus_tag="PK077_00200"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRN22752.1"
                     /translation="MNRKECLSSCRLYGIVDMGYTAEHQLLPVIEKLLAGGLRILQLR
                     AKNHNPEHIENMGRQLAPLCRKYGCLFIINDYPEIALNIGADGVHLGQDDGDLASVRG
                     LLGKDAVIGRSTHSPEQALGACGEQADYIGFGPLFPTGTKPGRQAIGLEDIASVQQQL
                     PENFPVFCIGGINGNTLPSVLEAGANRVVIVSWLLTHPDITGTVRTLRKELGEA"
     gene            complement(14980..15654)
                     /locus_tag="PK077_00205"
     CDS             complement(14980..15654)
                     /locus_tag="PK077_00205"
                     /inference="COORDINATES: protein motif:HMM:NF037008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRN22753.1"
                     /translation="MSSTRKSQSDRSAETSGKILRAAQKLFALRGFNGVTMRAVASEA
                     GVNLASIVYYFENKEGLYLAVYRQYAEPLMKARMKMLKEADLHPSLRAYARAFIEPAF
                     RLFLDESLGGPDHVRLLWRLPQEPEYLGRKIYDEFYAPSIQEMIARIRRFCPWDDELS
                     LSWHAHIMSSIFHATLGKYVACMDLQEKEPWMKDQEHILQMIVNTAVLLISRPALSPE
                     EEERQG"
     gene            complement(15682..16374)
                     /locus_tag="PK077_00210"
     CDS             complement(15682..16374)
                     /locus_tag="PK077_00210"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HRN22754.1"
                     /translation="MMGSSLCWADSSGAADDPSLRTWTNKNTGSTVEARPVALNMKTV
                     KLVTTAKKPITMPLEKLSDEDRAWLEEHKEVIGKPIAEWSSMPSGPVAEEMKGKTYML
                     ENGKLKKRDGKLNPKHFILYFSASWCGPCCRNAPHSVEAYNRVVKDNPDVEVIMCNMD
                     QNLDAAQKWAAANNMPWPILLKVDLTELAKKVAPRGIPTMILVDKDGKPIQSSQNMEQ
                     LVKAIGSSRSSR"
     gene            complement(16442..18721)
                     /locus_tag="PK077_00215"
     CDS             complement(16442..18721)
                     /locus_tag="PK077_00215"
                     /inference="COORDINATES: protein motif:HMM:NF025890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN22755.1"
                     /translation="MPAPESADAPVNRPSMKKWVVLLVVLGALIGLGTMMKAWMDRRF
                     GATAQPVVFSGGSEHWDQNEVPISMQCGACHEKEFRQWAGSDHAWAFRKLGDQWESEA
                     FHNMKLDAHGSILQFSTNGHGRMVRDGQSSTSWRAEWATGRIPLVQYLVPAKDGGFHT
                     LSAAWDVSRKEWFDIFGKESRQPGDWGHWTGRGMNWNTQCAWCHMSLFHKNYDPGKDR
                     YASTWTEPGVTCIQCHGPLLDKPEAGTGCMISTRNKLTPQQIHDNCASCHARRDEFDH
                     DFAVGDSFDNHFQLVLPVQPGVFWPNGMQRDEDYCETGLRLSRMGKAGVTCLDCHDPH
                     TGTLKLPQEDNTLCLRCHGTGEAVNGVKAPVIDMATHTPCPQSSMGARCVECHMPESL
                     YMARDPRRDHSFNSPDPLLSVELGIPNACTMCHEEKSNEWAAEAVKKYYGAKPKMAQY
                     RERTRAVQHAYEGKEDVLPLLIECFKREEVGAWRATLLDLMDAWAHEPAVQEVAAAAV
                     KDPDPLTRAAAAKVMGRVGNPAAGKLLNDPFRVVRLQAEWALRDSLPPDSPGMKELTA
                     AALHQADQPSGAMKLAQIAISRKDVKTAELWFSKALKWDATSSVVHRDYAVFLASQGR
                     SREAVDQMQEAVRLAPKDANLWYLLGLGQIENNDEPGALESFNEALKIEPSFIRALFN
                     RALLNEKVGRLEQALQDLENCSSLEKGNADIPFTLAVMLYRNGRYREAAAAAAEALRR
                     DPGHARARQILESASRHGR"
     gene            complement(18816..20141)
                     /locus_tag="PK077_00220"
     CDS             complement(18816..20141)
                     /locus_tag="PK077_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRN22756.1"
                     /translation="MMTVFIILLLIVLNGLFVAAEFALLGAPRAALEQMGVSGNMVAR
                     RVSQILKTPRLQDRYIATAQLGVTFASLGLGMYGEHAVAGWLHEWLAQYGWASWLVAH
                     GAASILSVAVLTYLHIVLGEMVPKALSLQYATKLCLIIAMPMYVIQLCLYPLVVGMNA
                     LGNFFLGLLGVDRNESKSHYHSAEELQYIIEESHENGALPQESGRIMDGLFDLDDLYV
                     HQVMTPRVRIDAIPEGAEHEQIREIVRRTRRTRYPVYRGDLDHVVGMVHARDLFRIMS
                     RRKALTPEYIHAIPKVPKTVKFDNVVEIMRKDNVRLAIILDEHGGTSGLLTLTDVFSE
                     VMGWDRGRIKVLTELPRDAVGFSCDVSGLARIEELGEAMDMDLEDGEIDTVGGLILNL
                     LEAPAEEGDTVGYRGLEFKVTRTENGGVERCTVTRITPLMREEVLEEEE"
     gene            complement(20138..21472)
                     /locus_tag="PK077_00225"
     CDS             complement(20138..21472)
                     /locus_tag="PK077_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRN22757.1"
                     /translation="MILFVTFVILFLILVNAFYVAAEFAAVSVRRNMIREMAENGSRV
                     AVHLLKILENTKELDRYIAACQFGITISSLVLGAYGQVELAAYLFPLFERFGGMDSVM
                     ANSAAALVVLIGLTVFQVILGELMPKSLALQFPKQAALYTYYPMRWTLAFFAWFIDFL
                     NGSGLLLLKLFRLPPGGHQHIHSQQEINMLLDESHQGGMLEEDEHERLHSALSLAERT
                     VEQIMIPRFQLVCLDVDATQEDILNMIADRPHTHIPVYEGNRESVIGMLHIKDMVAAY
                     AEKGILPPLRSMLRQVPCVMEMQTVEMLMARLREDRAKEAFVLDEYGKFVGLVTLERL
                     LGEMVGDIDEEFIRSGEKVENLPDGSVRIPGMMRAHKAECLVPFLMNGATTVGGCVIK
                     HMSCIPKDGDRLIIAGRVLVVEKMDHNRVSSILLLPPERKENEFAMESGVDA"
     gene            complement(21757..23901)
                     /locus_tag="PK077_00230"
     CDS             complement(21757..23901)
                     /locus_tag="PK077_00230"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRN22758.1"
                     /translation="MSIHSVECNVGTNPITIETGKMARLADGAVVVRSGDTVVLVTVV
                     SATKVKEGQTFFPLSVEYKEKAAAAGMFPGGYFKREGRPTEKEILTCRMTDRPLRPMF
                     PKGYFYDTQVITLLLSADGENEPDILSINGASAACVVSDLPFAEPVGAVRVGRIDGQF
                     VINPTNSQREHSQLDLVFAGTKDQVIMIEGSANELPEEDFIAALRVAQENVKVICEKQ
                     EELRAVCGKEKRAYELCLAKPELLEIGYEIAGDRIEEAIYAPSKVERQKKVGALRDEV
                     EAAIKERHPEATDFDVEQVFEYIQKKAFRISIMEKDKRADGRALKQLRPLTAEVNVLP
                     PVVHGSAMFARGETMSLCLATLAPMEERQYMDNYTGSVNEKRFILHYNFPPFSVGDTG
                     RFGGQNRREIGHGALAERSIAPVVPGEQEFPYAIRVSSEIMESNGSTSMASVCAGTMS
                     LLAAGVPLKRPVAGISVGLVTEQNDQHEITSYKTLLDIIGSEDFYGDMDFKLCGTSEG
                     VTGYQLDLKLPGIPLSILEEAIHVAKAGRTDVLKVMNEAIAAPAQMSPNAPRIETTKI
                     PADRIGELIGPGGKNIKAIQAESGADINIEEDGTVHIYAAKQEGLDRALELVTRMFKT
                     IEIGELYTGKIVSTTTFGAFMEVLPGKDGLIHISELAEGRTAKTEDVVSVGDVVTAKC
                     IGIDDKGRVKMSIRAALRDAKAAEAEAAGITE"
     gene            complement(24783..25037)
                     /gene="rpsO"
                     /locus_tag="PK077_00235"
     CDS             complement(24783..25037)
                     /gene="rpsO"
                     /locus_tag="PK077_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRN22759.1"
                     /translation="MSNEINLQEFKMHEKDSGSSSYQIARLTQRIAHLTEHLSTNKHD
                     VSSRRGLLKMVALRRKLLDYVKREDLAQYQSLIQRLGLRR"
     gene            complement(25139..25780)
                     /locus_tag="PK077_00240"
     CDS             complement(25139..25780)
                     /locus_tag="PK077_00240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HRN22760.1"
                     /translation="MASKDWNNMLSGRLYHAQDSELAAARLRARRLVFRYNQSAPENE
                     SLRRELARELFGQMGEGCYLEPPLRCDYGSNIRLGDRVYVNFNLVVLDCAAVTIGNDV
                     LIGPNVGIYTAGHPVDSGLRRQGLEFALPITIEDGVWIGGHAVIAPGVRIGRNSVIGA
                     GSVVTKDIPANVVAVGNPCRVVRPVTEKDREVWDTGENGEGPLSQGPKANSPG"
     gene            complement(25758..26621)
                     /locus_tag="PK077_00245"
     CDS             complement(25758..26621)
                     /locus_tag="PK077_00245"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRN22761.1"
                     /translation="MKLSLFHPLVSCLCVPFLFSSCGTTGRELSPVPSSHIPVEEMTG
                     YFEETGTIPGHLLKWEDDPSLPGKLLVVVDKKKQMMYIYRGTHRIAYAPISSGRSSGM
                     TPSGYFLIASKEEDHHSYYGAFINADGELRDGDIRRDSPRPGERFEPAKMPYFMRVNG
                     AVGIHEGYLPGHPSSHGCIRVPHLIAKNLFEIAPVGTRVVVRDGNWNIHELQQKPSGM
                     FRTVHKPAAPVAGANSASSGKKELVKSETSVKKDAAAMPSVGEQKNGQLPFSEADAVT
                     PSSSNGLEGLE"
     gene            complement(26738..28369)
                     /locus_tag="PK077_00250"
     CDS             complement(26738..28369)
                     /locus_tag="PK077_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22762.1"
                     /translation="MENSNHLTESWSLSWQSVLSSQLWRLEHLYWIENKAGQLQRFSL
                     NRAQRRLHERLWYRNDILKARQLGISTYVAMLMLDMSLFRSNFHCGIIDKSLPDAQQK
                     LAKLHFAWDHLDYVPPNPSAMDVALARLGERIKSLSGVEKKGEWQPRTLARSRLAFSN
                     GSDVRVGTNLRGGTMQFLHVSELAYVSVHAPWRAREIRTGAINTVPPGGFILKESTHE
                     GGRYGVNYELTRQAMENMGKSELSPLDFRFFFFSWFDQDEYTLPGRGRWSRELDEYFL
                     SLERETGVVLDAGQKRWYARMARVMGASMKQEYPGTPQEAFATGEEGSIYGSRIMALR
                     ERGRVGVEFEADPDAPTFTAWDLGLSDHTAIWLVQIMGDSFHWLDHYAASQQPLAHYV
                     EKMKDWEKNHGVAVTFHLLPHDAARRDAHGISYVENMGRLGLVNVRVVPRTTDVWRGI
                     NTLRELLERSFFHARTQEKARGFCGEEEPGGVEHLELYRSRPPGVGGAIAESPVHDGH
                     SHTADAARTFAEAWSHGLLHGTGNGRERGRRARMW"
     gene            complement(28356..>32877)
                     /locus_tag="PK077_00255"
     CDS             complement(28356..>32877)
                     /locus_tag="PK077_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22763.1"
                     /translation="GLEGLVASEEPVKEGFPAVQLNRKAVAGVPDVIRAVGCLASLED
                     GDRQKVFRMMAPYLRSRPAFRDALGGALKRGAAEVAENAAQLAVNGAAWLLPEGREKE
                     ALDNYSRSFEELRNFARMEFAPLRGGKDSPWFREMLVDMAQQGASTALAFCGPAGLGM
                     LMAGETGRHMAEARSMNPDGVFDAQMGGAVVSGGVNTLLSFGMTKLGQKMLGQGMRAF
                     RAMRPASGNPGAFFPGMGAVAADAVKMEAENKLMELTPMVVQEGVGALTNKASGVDWE
                     GWKARQASADDQLREAVMMMPFLLIGAGKVALNHFRHAGMLLGDGSPLKVFGIPDETV
                     SRILQDKDVNRAGELLQKSLRNSPLWGSLYISRKALEWSRALDEAGEPFLKTEQDVRD
                     FLDLPSPVRTKPWKMRDFPESEAVLKKLFPHPDYVTARTKWLLRAGLPRVGEAAAADG
                     QLVYAAEPAPGVEMKRTQGRPSVEEALSSWYETLGRMEEEDLGLVRNRNGREIPWRLR
                     NAEEYDIHADALRRAFVADRLKKRSYRPYRMLLLAYPEEAGRRAGFSGRDWDAVTEDM
                     DLRTRANVYEGVMERVHGVPHEEASGHVAFRLWKSFCRDEGACGRARRWLEEGSALLN
                     APDLLSESFNPDGLPSALARMSGMMASLGGEERHGVSPELREMNRMVWGTQADVNSLY
                     HVLPNMKEFDVHVGRGYTPVESYGRLLARFLEVAPENVARYASVLDAPRLHAGPEQVS
                     PRIYPGTRKAVESLLLLTPRLFQSSPGGEGAERLWRVKYPNGRWSDWHPSREAAEADL
                     MANVSMMFSAACSAKRDLIRSWEWHAHNGRPEWDTNYLRLTTGLENVQGGRLPCLYDS
                     LTAMAVADMLKAGYGVRGAVGSGDRLEVTGRGRLAAETLMSRDALLERMNIHEKYVGS
                     VQRLGVDADNLPVSSAKGLVMHRLRLHNTANPLALIEDKAEVVWDRLMRTDQLSPEGA
                     WEMLKHLERVPRRRKLPGETELIEELSQLSKEYFFAHLDHDTVPETVASWARYGAAMP
                     DRVPEPLEELVRRAGLLKKTLREDADTAEFMGMLRETVGMNERIRAERAWDPAGGEVR
                     LPMVERYSHSLMSGRLLFTVPDHVKEGLVRSLSALDGYAGGSVRRMERLAESRMRELS
                     RALQEFPQLNSWRPDPEHPGLYLHLVKKPFRTGLGPLRAKDVSLEALGEHLPEPILEA
                     PLYVEEDFSVRRGVPAPAVWRSRPDVVRAVETLDAVRRDFAGRPEATESGVSWKGKIY
                     RIDSGAAPGGVTSVWNRELPLDEAISLIGVLDRRQEELRGGMLPFLPEPEEARAMYAN
                     CVIYRDPDDPGHTVRLMPGIPESPVRQARAPYVVHAWNGVYLDSRGAPAVSERDSYIP
                     LECFTGAGEEPSPEDSRASRTRFLGRILETLGDADAQERWWWNRRQAVGCFMEDVIRM
                     YEELGVRESYLKGKLDLLDPVMVQALRFVSHVLNDPLAYRLPLDKGTEAQKGMTREGA
                     LLKELIEEYGKF"
BASE COUNT         7959 a         9753 c         7770 g         7357 t
ORIGIN      
        1 accccgcctg aaatcgcaaa taaacacgct catccgcagg accggccagg gaaagggggt
       61 gggggcatgc cgtcttcagg tggtaggact gttcttggca gaccgccgtc gcccaggctc
      121 cgggacacgc cagcatgccg gtgcaggccg ccagcgacaa cagaattacg gtattcatct
      181 tcatattggt catggctgga aatttttcta aaacggcata tccggaggcg acgccatcca
      241 cccatgcgta cccgcacacc gctttggccg taaacggata ctacgcccgc acccggacaa
      301 ataccttcta aagatgagac cagcataaaa aaacaatatt cagttccaag agccaaaaaa
      361 acgaattccc catgaaagat tgtgtcagca gtctttgtac ctgtaaccgt gtccatgaaa
      421 ttcattatcc ccgcactcgc cctgtccgcc ttccttcccg cagccttctc acaagaacaa
      481 atcatcccga aaccggcgga aatcacattg ttcacaggaa gtccggcccg tctcacaccg
      541 gattccctca tcatcacgga aacacaggac aaagctttcc tggatcaggc agggcaattg
      601 cagcagatgc tcagcgcagg gacgggactc ccccttcccc ttaaaccggc cgggcaagcg
      661 tcgaaaaaag ccgcctgcat tgtcatcaaa aaagacccag ccctggccgc caggggagaa
      721 gaagcctact ccatccaatc atccccaagt gaaatcatcc tttccgcagc cgatgccagg
      781 ggcatctttt acgcggggca aagcctggtc cagatgatgc cctccgtctt ccacgaccga
      841 acggcggata aatccgccgt ccggtggaat atttctgaaa ctccgttccg cataacggac
      901 tacccgcggt tttcctggcg ggcgctgatg attgatgaag cacgccactt ctttggcgag
      961 aaaaccatta aacagatcat cgaccaaatg gctctgctga aaatgaacat cctgcactgg
     1021 cacctgacgg acgacacagg atggcgcatt gaaatcaaga agtatccgcg cctgacctcc
     1081 atcggctcca aacgcaggga atcagaaatc ggcacatgga acagcggcaa gtcagacgga
     1141 acgccgcatg aaggctttta tacccaggaa cagatcaggg atatcgtaca atacgcagcc
     1201 cgccgaaaca tcaccatcgt tccggaaatt gaaatgccgg gccatgccag tgccgccgcc
     1261 gtagcatacc ccttcctgag cctgaaaact cccggggaag tgcccaccac attcatcgtc
     1321 aatacggcct tcgatcccac ctcggaaaaa acttatgcct tcctgtccga tgttttggat
     1381 gaaatcacgg cactcttccc cggcagaatc atccacattg gcggagatga ggtgcgctat
     1441 gacaagcaat ggaagggtgt tccggaaatt gaggaattca tgaaaaaaaa cggcatgaga
     1501 agttatgcgg acgtccagat gcatttcacc aaccgcatgt ccggcattat tgcccaaaaa
     1561 gggcgcagca tgatgggatg gaatgaaatt tacggacatg acgtcaatgg ggacggagga
     1621 ggaaaagccg gcgccaaact ggatacaaac gccgtcatcc agttctggaa gggcaacacc
     1681 ggcctggcta aaaacgctat ccgggacgga cacgacgtta tcaattctct ccacacctcc
     1741 acctatttgg attacagcta cggcagcatt cccctgcaaa aggcatacgg gttcgaaccc
     1801 gttttccccg ggttggagga acagtaccat tccagagtca agggattggg cgcccaagta
     1861 tggacggaat ggatttccac accggaacgc ctgcactacc aggcattccc ccgtgcctgc
     1921 gcctttgcgg aagtcggctg gactcccgcc ggtaaaaagg attttccgga tttcaaaaaa
     1981 cgcttgaaag cgtatagcga gcgtatggat ctgatgggga tcaagtttgc ccggaacgtc
     2041 atcagccaaa tagacaaatc tgactttttc aatacgccca ggatcggcac atggacgcct
     2101 gccaccctga cccgggagga acattcgttt gacgttacca aactggtcaa agcgtccggc
     2161 aaacacaccg tcaccctgct gtacgacaaa ggtgcccacg ccatcgaaat cgaatccgta
     2221 gccctgtatg aaaattcccg ggaaatctcc cgggacgccc atgcaggcag aagcggcgcc
     2281 tataaggaaa atatccagta cattctgaat gccccggaac ccaggcaagg agcaacctat
     2341 actgtcaaag caaaattcaa aggggacgga ggccgggatt cccacgggac agtgtatttt
     2401 gaaacgccat aaaaacaccg tccccgtcct ataccagaaa cagcaggggc ttcacacaga
     2461 acccccttta ctccgacaaa cggagcactg catccgcaga ctcatggtta cggacacaag
     2521 cctttcagta tcatcggcct cccaagggcg ccggcatgtc ttttcataga ggaagacgcc
     2581 ccttccatca agcggcacga aattgctact ttaaaccaga aaggcttacc ctgaaatcag
     2641 ccttgcctta acaaggctcc ggaggtcgtt gcttacgaac ctccggagac tgaacaaccg
     2701 tgaatcggcg gaagatgatt ttcatccggg aaagccagac ggaatttaca tgttatcatc
     2761 taccactttt tccacatact ggaaaaagat gcttccgtcc tccccgctga ttccatagtc
     2821 tctgacatcc tcaatattcg cattcttgat tcgggtaata atatagactt tggagtacaa
     2881 aatcccccat tccccatact ttttcttatc aaaccagata tggtggttat agtatggttc
     2941 aaaatagatg gttcccccgt ctatcggatc ggaaatcggg ccggaatatt tgacaacaat
     3001 atggctgatt cccttgtaac accattccct gccgcattga ggatttttgg aaagggtggt
     3061 gctcatttca atggtggtgt ccatgacatc ataggatcct catcatggta tttgattcct
     3121 ttttgaggca gattgaactg atttttgatg atgactccat cataaatgag acgtccccct
     3181 ttccattccg ttttctgcgg cactgtttcc cctttcaaga gacagaaacc attattgcac
     3241 aggcaatttt attcattgtt attttctact ttgttgttgt ttcagcaatt ccttattgag
     3301 gaattctttc atttcctgaa cagttttttc aggaggggtc ttcgcatcgt cccgatactg
     3361 cctccagtcg atgcgcagct tggccatcag gcgcaattcg ctgagagaaa aaggaacagt
     3421 acccggggca ttcggcatga aatcccggga cctgttgcca atataataaa tctggctctg
     3481 atcccaagaa cgcttcatgg tctcatcgaa ggaagctctc acacggtcgt cgcttactct
     3541 ggcttcccac tggaggcagt ccgccaggca accaccctcg gcccgcctct cccgggcctg
     3601 ccgccacagg gaaagaatat acctgttctg aagactgcac gcagctggag tcaggtactt
     3661 gcgcgtatac tgcaactcat acagaggctt gcacacagcc ttttccctct gtcccagagt
     3721 agctccactc accctttccg gagtggggac aataccggcc agagcaccgg atacgtaata
     3781 aggcaattcc tcaggcattc gaatttccca cggcattttt tcattcacaa acacgccgtt
     3841 taaaatggta ttaaaggaac cgtttcggca aatacggaac aggtaaatcc cccctttcct
     3901 gctcatgaac cttttataaa ccccggaccc agcaaatctg gaaacgcgtg tgcgcgcctc
     3961 caccgggtag gaataccact gctccatgga tgcagcaagt tccttccccc aattattctg
     4021 ttctcctatn nnnnnnnnnn nnnnnnnaag atccccctca atattgaact ggaacacaag
     4081 gggagcggtg gaagggtgcc ttctggcttc caccacgtaa tcccgcctgg attcattggt
     4141 gagataaccg ttgggattgt aaaaatacag ggctatttca tgaacacctt ccggcacctc
     4201 tacggctacc catatatcgg gtccatccac aaagcccgga tacacttctc cgtggtcatc
     4261 ccactccgcc tctgtccgca cggaatccgt agggtcgaaa agaacgttgc ggttatcatt
     4321 ttccttgatg ctatgcaccc accagcgcaa agcgtcccct ttcttgcggt tgagccccat
     4381 cagcccctga atccagtagg gtgacaaggc ccccttgtat ccgggatgtc cgggaacgga
     4441 agaaagggta cggaacttga taggcttggc tttgaattcg gaattgctca tgggggcatt
     4501 cgtggcgcac agcgtggcac gcgtgtgccc gtacttcccg caccagtccc cttttgtagt
     4561 ccagtcctcc ccccggaaca caatattctt ccccgcatgc gcatcggatc ccagcctttc
     4621 caaccttctg agcactacag aaggctcctc catctgcgcc ggacgaggga acgggacttc
     4681 attgtccagc tgttccctgt ccagtttata agccagagtc ccctttttca gacgaacaaa
     4741 gccccgtccg tatgtcgccg cccaaacact cccgtcctga agcgccagca aggaaggaac
     4801 atccacgttt tccagcccct tccttattct ctgattcacc gtcatttttt cagcatccag
     4861 aacatccact ccctgcatcc ggtagccgat gaaaatttcc ttttcctgct ggataatgga
     4921 agaaataaaa tcttcagaca acattctgga attcgactcc cccggcaccg cagtcttccg
     4981 tgcggcagaa ccatacagat ggttgttttt ctgggcataa tcacgccctc tcatataacg
     5041 ccaagaacta atgccgttac tgtacgccaa gcctgagcag gtacctacca acacctggcc
     5101 cttatccgta caagtcaaaa cattgcagat attggaaggc aaaccatcac catacggctg
     5161 gtacggctga cgggcgaatt gcctgttatc ccagtaccag ggagcctgaa catttttcca
     5221 ctgcatatac tttgactggc gcgaagagca ggataccccg ccgcaagcat aggccagcca
     5281 caaattcccc ctggtatcaa aacctgcgga agccgcctgg tcttccacca ggccggtgga
     5341 cctgtccagc gtcttccatg aatcgcctgc aggatcgtaa atggatacgc ctccggatgt
     5401 ggcgacggcc acctccccgg aaacgggaga aatggccaaa gcataaacat gctctccatt
     5461 cagggcattg ctgcggtcat acatcttcca ttcccttccg ttaaaannnn nnnnnncaga
     5521 tgcgcccctg cctgtcctcc gcgatacagg tgaaattctt tcctttgggg aaaccggaat
     5581 aataatccat attaagccac gatttttcat atgtccggtc cccaatctga aggcggtaaa
     5641 tgctttcttt ttctcctacg gcccaaatgg agccgtcgcc agctaccagc gtgccgcgaa
     5701 taaaacggac cggacattcc ggaagttgct ccggtatctg gacggttacg acctttcctg
     5761 ccgcccatgc ccccaacaaa aggaaaatca acacaaatgt ccctacgaag tgtttgaatt
     5821 taatcaacat aaccaactat ctcctgatta ttgcaggata atattatcgg tttttatcat
     5881 tgtcaacgcc attttcaatt tattgtcaag tttatccaaa caaaatacgg cataattcgt
     5941 ttagaattat gcacataaac aatttaatat atgttagtta tattaaaaac atcaattcca
     6001 agaaaatcct cactaatact tttttatgct tggatgggaa aatacaggga acaggcctga
     6061 tagcaacgtc cggcattccg ttttttcaac atgttttccc cggttattct ctgattcaaa
     6121 tttcaaacct accatgcgtt tccccatata aagttatatt taccggcctt ttttgaggca
     6181 ggagaataaa cacagctcaa gatccgaagg agaagccatc tactcttccg aagcggcaat
     6241 aggaggcaat atggacgttc ccggaggagg gcttcccgat gatggccaaa gaaaaagaaa
     6301 gtgttgtcct caccaacacg aacaattctt ccggacctga ttccagcaac cagttccggc
     6361 atagtcacgc agcacccttc cgaccatgaa tgaggtattg cttcataaag cgggactgac
     6421 ggtcaactgt atattaaagc caccaaagga aaggagggag gaaccagtcc gacaggcgga
     6481 aaagaagaaa gcgaagacaa aaaagatgga ataaagcccc cggttttctc cctagatggc
     6541 ctatccgggc gattataata gcggtctgca ggtttctgtt gagccccaag ccgggggtat
     6601 gaaacatcgc ctccctaaaa gatacggcca aattcacgac agatagaatt atgccgtccc
     6661 gtacgttctt tcctcatacc caggtgccaa ttattacttc tagataaacg gaaaaaacga
     6721 acctccctga cctatccaac ccctccggat tatttctgga gaggaaggtg ttccgttcca
     6781 caacaaagaa aaggaacata tcattctgca tccggcccat agcagaacag cctttccgcc
     6841 ccggattacc tccctggggc tgttgaatac cctgctgaaa tatcgaaacg tatcaaataa
     6901 acacttatta aannnnnnnn nnaaccttga caactgcgga gctgcgctta cacacactta
     6961 ttcatccaac aaaaatcgct cagcagagct tcttccatca ggaatggtgg aaagccgttc
     7021 cttcttccgc cgtcatcgga gaagagaccg ggtgtttctc gaagaaagag agcatgcgct
     7081 tgtagtcttc caggaacagg tctgccaaat ctttggagaa gccctggcga accatgacgc
     7141 gcatcaccac cgtatcctga cagttggcag ggagcgtgaa ggcgggcacc tgccaaccgc
     7201 gggttctcag cttgtccgaa aggtcaaaga gcgtgtagtt cgcacgggct ccatccttga
     7261 tgaagaagca gacggcggga ataccttttt ccggatcgcc cgtacaaatg aattcatacg
     7321 gccccagttt ggaaagctca tctgaaatgt acttcgctac ttcgtaggcc tgtgtatgga
     7381 tgttcgtata gccttccttg cccagtcgga tgaaatcata atactggcag ataatctgtc
     7441 cggccggtcg tgagaagtta atggcaaaat tgggaatttc cccgccaaga tagttgacat
     7501 tgaaaatcaa cccttcaggc agttcggaaa tatctctcca aacgacccag cccactccga
     7561 gcggagccaa tccgtattta tggccggaag cactgatgga cttgacgcgg ggaatgcgga
     7621 agtcaaagac aagatccggg gcgcagaacg gagcaaggaa accgccgctg gctccatcta
     7681 catggatgtc aacgctgatg ccggtcttgt tttcataatc atccagggcc ttggcaattt
     7741 ccgccggcaa ttcataagca cccgtatagg tcactccgaa cgtgggaacc acaacgatgg
     7801 tgttctcatc aatctgttcc agcatccggt caacatccat cttccatttt ccgggcgcca
     7861 tcgggatttc acgcatttca atatcccaat agcggcagaa cttgtgccag cagatttgga
     7921 cagggccgca taccaggttg ggcttgtcaa tgggtttgcc ggcggctttc cggcgggctc
     7981 tccagcgcca cagggcggcc atgcctccaa gcatgcatgc ttcactggaa ccgatagtgg
     8041 aacaaccaat aggcttttca ttctggctgg cattccaaag attggcaata atggcagcgc
     8101 agcgcatctc aatagcggct gtctggggat attcatcctt gtcaatcatg ttcttgttaa
     8161 tgctgagatt catcagcatc ctgacctgct tttcatccca ggtctggcag aaggttgcaa
     8221 gattctggcg ggcattgccg tccagcatca attcatcgga caccaattgg aaagcgtcct
     8281 ccgcgcgcat cgcataatcc gggaactcat ccttggggag aaggttatct gcttccggag
     8341 aaccgaaaat ggaattttcc gcccgggctg tatcgggttt ttgatttgga tcaaacatga
     8401 cggtatctat tgtttcatcg tttccgggcc aaccgtgaaa ggcaacccgc atgaaggata
     8461 gaagcacccg agaataaaag cgttcatatt tccattgtca ttatcctagg tataattttc
     8521 ccggaaaaac acagagtcga gatttatttc atcattcatt gatgactaaa aattatttaa
     8581 ttgcaaatat ctttataaaa actcgccaat atctccagat aactttatgt atgacatctc
     8641 catattatga cagttaagag aaacggacgt tttcggatgc aattcccaga cgggataatg
     8701 tgttaccccc ctatcttaac aatacaacat ccattggaaa aaactgtgtc acggactttt
     8761 acggttcctg atgccgaggc gtgcatgcgc actttcagaa agggaaaaat acggattaac
     8821 ccgaatttcc atcaaaaaaa ctgctgcaca ataataaaac caacatcgtg gaatcttccc
     8881 cgcttttcct gatgagcaac ttctggtgca gacaggggga agaaatactc aacctatacc
     8941 gggttaatgc cgtggcaagt tacacccttt taagccacgg ctggcagcag aggtgccttc
     9001 tccccctccc tgcacttttt acccggtcaa caggtggcat tccttcaaga ttactcccgt
     9061 tcaattttcc ggaaggtatc cttcacataa agtacatgcg gataaagaat ggaatccgga
     9121 gctactttgg gatgattgaa ttccgctatt tttcgcattc caacacgttg catcacagct
     9181 tgtgaaggaa aattcacgca agcagtaaaa gaatataccc tgtcaaagcc gaatcgtgca
     9241 aaaccatgtt tcaggcatgc acgggcgcct tccgtagcgt atccgtaccc ccaggcttcc
     9301 ggcacaagcc tccagcctat ttccacacac ggtgtaaagt ccgcttcaaa atcagcccaa
     9361 tgaaagccga gaaggccgat caaggaaccg gaatgtttca attccactgc gtacaatcca
     9421 taccctcttt catccagttc ttcccgcata cggttccaca tggaaagaga atccttgtcc
     9481 gtcatgacac cgggaaaaaa acgcatcacc tggggattgg cgttcatagc cgcaaaaaaa
     9541 ggccagtctt tttcccgcca ctcccgtaac accaggcgtt cagattccgg caatgacagt
     9601 tttcctcccg gcatggcctt agttgtacat ggaaagctct gtttccagct ccgggcagcg
     9661 attcacgcga aaccgttcat ccagttccag aatttggcta tggcccaggc tgtccttgat
     9721 ggtgagattg actttggaac gaccgggata cctttttaga atatcccgta tctgttccag
     9781 attttttctc gtgtggcgga ggggactgac aatgatattc agaggcccct gatccccgga
     9841 tttggatttg cgctttttgc ctccaagtgg gtcaatgctc tgggcagcca cgcttttgga
     9901 ggatgtacgg tcatcctccc ggatgttggc tcgcatggag acgaaaacgc ccggctccag
     9961 gcctccctcc atttcctgag ccttttcata aacatccgac cacaacaaca cttctatgct
    10021 gccggtaaaa tcttccaacg tcagaatggc gaattttcta ccggccttgc tgattttggg
    10081 ggtgacggaa cgaaccatgc cggcaatctg gtgcttctgc ttgagttcgt gtggttccag
    10141 cgtgtcaatg agtcctatgc gggtatattt ttccgcatca atgacaccgc gcatggagtc
    10201 caggggatgg ccgcaaaaat aagcgcccaa cagatccttt tcatctccca tacgctgctc
    10261 cttggaccac tctggaacgg caggttcatc cttcaccctg ggcgcttcaa aggtcatgtc
    10321 aaacagagac ccctgcccca gagccttatc cttatgtacc tgggaagctc cgctgagggc
    10381 caggtccaca cgttcgaaca gaacacaacg cggctccagc gtccagtcca gcgctcccgc
    10441 acgaatcagg gattccagaa tctttttgtt gacagatttg gaatccagcc tgttacaaat
    10501 gtcctccata ctggaaaatt ccccattctt ctctctttct tccagaagaa tctgcatagc
    10561 cccctctccc acgttcttaa tggccgccag cccataacgc acggccagct tgccggaggg
    10621 cattttctca ggagtgaact tgagcatgga tttattcaca tcgggaggca ggatctcaat
    10681 acccatgcgg ttggcttcct gaatgaacac tccgattttg tcattgttcg cctcattaga
    10741 catcagcccg cagaggaatt ccacagggaa gtgggccttg agataggccg tccagtagga
    10801 aatatgcccg tagcaggcgg agtgggattt attaaaacca tacccggcga atttttcaat
    10861 cttgtcaaag atagctgtag ccgttttttc atcaatctgg tttgttttcc agcatccttc
    10921 cacaaagtgg gccttttcct tggccatctt cgccgggtct ttcttgccca tggcgcgccg
    10981 cagaaggtcg gccccgccaa gcgtatagcc ggccagcagc ttggccgccg cctgcacctg
    11041 ttcctggtag atcatcacgc cgtaggtttc cccactgacc tgttccagca aaggatgctc
    11101 atactccacc tggcgcagtc ccttcttcac ttcaatcatt tcatccataa actgcatggc
    11161 tcccggtcgg taaagagcca gcaagtcaat gatgtcttca attttctgaa tcccgtaacg
    11221 tcggcaagtg tccaccatac cgccggattc caattggaaa acccccatcg tatctcccct
    11281 gttcagcaag tccagggttt cccggtcatc cagagggacg gaatccagtt tgaactccgg
    11341 cacgcgccag cgggcgtatt gctccgcatc atgcatgacg gtcagggttt tcaaacccag
    11401 gaagtccatc ttcaaaagcc ccacttcata aatcgcgctc atgtcgcact gggcgacgac
    11461 agcggcatga ggatcggaca gatcgtcacg ggtgagggcc acatgctcgt ccaggggacg
    11521 gtcaccgatc acaacacctg ccgcatgtac ccccacgttg cggatcaagc cttccaggcg
    11581 ggtcgcgtac ccccacaatt ccgcataccc ctcatcagag gcaatcaact ccctcagctc
    11641 ctcgttactc ttgtagctgg actgaagagt caccttgggg ccggtttcga taagtttgga
    11701 aatacggtcc ccgtccgcat aggacatatc catcacacgg gctacgtccc tgagtacgga
    11761 cttggccccc atggtgccgt acgtaataat gtgggaaaca gcgcgctctc cgtatttctg
    11821 ccgaacataa tcaattactt ccggccggcg tgtctggcaa aagtcaatat caatatccgg
    11881 agggctgacg cgttccggat ttaggaaacg ttcaaagatt aatccgaatc tcagaggatc
    11941 tatgttcgtg attttcatgg cataagccac caaggaaccg gctgcagaac cgcgaccggg
    12001 acctacggga atgtcatgat ccttggccca gttgatgaaa tccgccgtaa tcaggaaata
    12061 ggagggaaat ttcaactggt tgataattcc aagttcataa tccagacgat cgcgcaattc
    12121 cttatctgca gctacacgtt cctttccgta ccgttcttcc agccccttat agcatacttt
    12181 acgcaggtat tcttcgcgcg acgacccgtc cggcgtgccg aattcggggt atttctccgt
    12241 actggtggaa tccagcttga tggtaacatt gcaccgttcc gcaattttca acgtattttc
    12301 cacggcctcc ggataatcct tgaacacctc ccgcatttcc tctggagatt tcaaatacac
    12361 ttccgtggaa tagcgcatgc gttttgggga agccagtttt tcgttggttc cgatgcaaat
    12421 cagaacatcg tgcatgtcgt gatcttcctt cctgaggaag tgcacgtcat tggtggcaac
    12481 aatgggggta ttggtcttgc gggcgatacg caccagctcc ggcaggcatt ttttctgttc
    12541 ctccatgcca tggtcctgaa gctctacaaa aatattgtcg tccccaaaaa tatcctttag
    12601 cgccagaagg gcttcctctg ctttttcagg ctggtcattg agcagccact cattaagcgg
    12661 tccggcaatg catgctgtac aacagatgag gccctcatga aattccctca gggtttccat
    12721 atccacgcgg ggtttataat aaaagccttc caagtgggaa cgggacacca gtttaaccag
    12781 attctgccac ccggtttcat tttccgccag caaaaggaga tgtgtgtatt tgcggcggcc
    12841 gggaagctgt tttttcacgc tgatgggcgt cggggaaagg taaacctcgc aaccaaagat
    12901 aggctttacc tcctgcttct gcggtccttc cggatgctcc ttgttccagg cggccacgga
    12961 tttattgagg ctgtttgtct ccatgaccag ttcaatggat ccgaacaaat tgccgtgatc
    13021 cgtaacagca acggcgggca tccccatgcg ggaacactca gcaatgaggt ccttgatatg
    13081 gacggcgccg tccagcaacg aatattccgt gtgattgtga aggtgtacga aggaaccggg
    13141 catggatgga tcctgtaagc ggttaataaa ggaaatcagc ggtgaacagg gcattcggcc
    13201 ttcatggctt ccacgcattc ccggataagg tccgggccgc gataaacgaa gccggtataa
    13261 agctgcacca ggctggctcc agccttgatt ttggcgacgg catccgcccc gttcataatg
    13321 cctccaacgc caatgatcgg aatacgtcct ttcaattcgg aagcaaaagc tccaataact
    13381 tccgtggacc gttccgtcag aggagcgccg gacaggccgc ctgcttcttc atgccgagga
    13441 ttggcctcta ccccgacgcg ggaaagagtc gtattcgtag caataagacc atccagtccc
    13501 tcatccagaa aaacgcggct gagctccgca atatgctcat ctgtcacatc aggagccact
    13561 ttcataaaaa tgggcacatg gcgacccgtt tccgcagcca ggttggattg ctccgatttc
    13621 agggaagcca gcagacgggc agcgggttcc gccgcctgaa ggtcgcgcag gcctggcgta
    13681 ttgggagaag agaaattaat ggctatataa tccgccacag gccaagccgc acgcagacaa
    13741 gtaaggtaat cctgggcggc atcctcattg ggagttattt tatttttccc aatattgaca
    13801 cccagaacac cgtgaaaacg agtggcggaa cgaatgtttt ccactccggc ggcaatacct
    13861 tcattgttaa accccatccg gttgataata gctttctggg gaatcagccg gaacaggcgg
    13921 ggcttttcat tgcccggttg cggccggggc gtcagcgtac ccacttccac aaaaccgaat
    13981 ccggcctggc caaaagcgct taccgtatcc gcttccttgt ccattccggc ggcaagtccc
    14041 acccggttga ggaatttcat ccccaggatt tccacaggat ccgaaggaac ttcccccatt
    14101 actagaggca gtacacgcat tttttctgcc aggcgcaatc cccacaaggt caccttgtgg
    14161 gcagtttccg gattcatttg gaataataca cttttagcgg cggaatataa ggcgggtgac
    14221 acaatgcggg gagtgtatca caccctcttt tagggtcaag aatgggaagc gtcttgccgg
    14281 gatgatttta tccggtacaa ttccttgcca tgaacaggaa ggaatgcctc tcatcctgcc
    14341 gtctgtacgg tattgtagat atgggatata cagctgaaca tcaactactc cctgtaatag
    14401 aaaaactatt ggcaggcggc ctgcgcattc tccagctaag ggcaaaaaac cataacccgg
    14461 aacacatcga aaatatgggg cgccagcttg cccccctgtg ccgtaaatac ggctgtcttt
    14521 tcatcatcaa cgattatcct gaaattgccc tgaacatcgg cgcagacggc gtccatctgg
    14581 gacaggatga cggagacttg gcatccgtaa gggggctgct gggaaaggat gccgttatag
    14641 gacggtccac gcatagtcct gaacaggccc tgggtgcatg tggagagcag gcggactata
    14701 tcgggtttgg gcccttgttc cccacgggta ccaaaccggg aagacaagcc attggcctag
    14761 aagacatcgc aagcgtacag caacagctac cggaaaattt ccctgtcttc tgtatcggcg
    14821 gcatcaatgg gaacacactg ccctccgtac tggaggcggg agcaaacagg gtagtcatcg
    14881 tttcctggct gcttacacac cccgacatca cgggaacagt cagaactctc agaaaagaac
    14941 tgggagaagc gtaacgaaaa acgcgcaccc ccattcatgt tatccctgcc tttcctcttc
    15001 ctccggagac agagccggac gggagatcag aagaacggca gtattcacaa tcatctgcaa
    15061 aatgtgctcc tgatccttca tccacggttc cttttcttga agatccatgc acgcgacata
    15121 tttccccaac gtagcatgga agatggaact catgatatgc gcatgccaag ataaggaaag
    15181 ctcatcatcc cacggacaga atctccggat tcttgctatc atctcctgga tagatggagc
    15241 ataaaattca tcatagattt ttcttcccag atattccggc tcctgcggca aacgccacaa
    15301 taaacggaca tgatccggcc ctcccaggct ctcatccaga aatagccgga aagcaggttc
    15361 tataaatgca cgggcatacg ccctcaggga cggatgaaga tccgcctctt tcagcatctt
    15421 catgcgcgct ttcatcagag gttccgcata ttgtctgtaa acagccagat acagtccctc
    15481 cttattctca aaatagtaaa cgatagaagc cagattaact cctgcttcag aagcgaccgc
    15541 cctcatggtc acgccattaa atcccctgag ggcaaaaagc ttctgtgcag cacggaggat
    15601 tttgccgctg gtttccgcag aacggtcgga ctgggatttg cgggtagaag acatctcctc
    15661 aacaatcaga caatgcgccg cctagcggga agaacggctt gaaccgatgg cttttaccaa
    15721 ctgttccatg ttttgagagg actgaatagg cttgccgtcc ttgtccacca gaatcatggt
    15781 agggataccg cgtggagcca ccttcttggc aagctccgtc aaatccactt tcaacaggat
    15841 aggccatggc atgttattgg cagcagccca tttctgggcg gcatccaggt tctggtccat
    15901 attgcacatg attacttcca catccggatt atcctttacc accctgttat aggcctccac
    15961 gctgtgaggc gcattccggc agcaaggacc gcaccagctt gcactgaaat acaaaatgaa
    16021 atgcttggga ttcaatttcc cgtccctctt tttaagtttg ccgttttcaa gcatgtaagt
    16081 ctttcccttc atctcctccg ccacagggcc ggacggcatg gacgaccatt ctgcaatagg
    16141 cttgccgata acttccttgt gttcctccag ccatgcgcga tcttcatcgg aaagcttttc
    16201 cagcggcatc gtaatgggct ttttagccgt cgtcaccaat ttgacggtct tcatattcag
    16261 ggctactggg cgggcttcca ccgtactgcc tgtattttta ttagtccaag tgcggaggga
    16321 aggatcatcc gctgctccgg aagaatccgc ccaacagaga gaggacccca tcatgaatgt
    16381 ggatgccaat aagatacacc aagatgaaaa tttcatacac ataatgttaa tatactattt
    16441 cttatcttcc atgtcgagaa gctgattcca aaatttgcct ggcccgggca tgtcccgggt
    16501 cgcggcgcag ggcttccgcg gcagcggcag cggcctcacg gtaccgccca ttgcggtaaa
    16561 gcatcaccgc aagagtgaaa ggaatatccg catttccctt ttccagggaa gaacagtttt
    16621 ccaaatcctg caaagcctgc tccagccggc ccactttttc attcaacaga gcgcggttaa
    16681 acaaagcacg aataaaggaa ggctctattt ttaaagcttc gttaaaggat tccaaagctc
    16741 ccggctcatc attattttca atctggccca gtcccaggag ataccacagg ttggcatcct
    16801 tgggagccag gcgtacagcc tcttgcatct ggtccaccgc ctcccggctg cggccttgag
    16861 aagccaggaa aacggcataa tcacggtgca ccacggatga agtggcatcc catttcaaag
    16921 ctttggaaaa ccacaattcc gcagttttca catccttgcg gctaatggct atctgggcca
    16981 gcttcatagc tccggaaggc tgatccgcct ggtgcaaagc ggcggcagta agctccttca
    17041 ttcccggact gtccggaggc agggaatccc ggagagccca ttccgcctgc aagcgcacga
    17101 ctctgaacgg atcattaaga agtttgcccg cagcaggatt acccactcgc cccatcactt
    17161 tggcggcggc ggctcgcgtc agaggatccg ggtccttaac cgccgccgcg gccacttcct
    17221 ggacggcggg ttcatgagcc catgcatcca tcaaatccaa taacgtagcg cgccatgcgc
    17281 ccacttcttc cctcttaaaa cattctataa gcagaggcaa aacatcttcc tttccttcgt
    17341 aggcatgctg aacggccctg gtccgttccc tgtactgcgc cattttgggt ttagctccgt
    17401 aatatttctt taccgcctcc gcagcccatt cattgctttt ttcctcgtgg cacatggtac
    17461 aggcattcgg aatccccaac tctacgctca gcaaaggatc aggagaattg aaagaatgat
    17521 ccctgcgggg gtcccgagcc atatacaggc tttccggcat atggcattcc acacaccggg
    17581 cacccatgct ggactgagga catggcgtat gcgtagccat gtcaataacg ggagctttta
    17641 cgccgtttac cgcttctcca gtaccgtgac acctcaagca gagcgtatta tcttcttgag
    17701 gaagcttcag tgttcccgta tgcggatcat gacagtccaa acaggtcact cccgccttgc
    17761 ccatacggct taacctcaga ccagtctcgc aataatcctc atccctttgc atgccattgg
    17821 gccagaaaac gccgggctgc acgggaagca ccagttggaa atgattgtca aagctgtcgc
    17881 ctacggcaaa atcatgatca aattcatccc tcctggcatg gcaggaagcg cagttatcat
    17941 gaatctgctg tggcgtcagc ttgttcctgg tggaaatcat gcaacccgtc cccgcctccg
    18001 gtttgtccag caaagggccg tggcattgaa tgcaagtcac tccgggttcc gtccaggtag
    18061 aagcataccg atctttacca ggatcataat tcttatggaa caaagacatg tggcaccacg
    18121 cgcactgtgt gttccaattc atgcctcgtc ctgtccagtg tccccagtct cccggctggc
    18181 gagactcctt gccgaaaata tcaaaccatt ctttgcggct cacgtcccat gcagcgctca
    18241 aggtatgaaa accgccgtcc ttggcgggga ccagatactg caccagggga attctccctg
    18301 tggcccattc cgcgcgccag gaggttgaag actgaccgtc acggaccatg cgcccgtgac
    18361 cattcgtaga gaattggaga atgctgccat gggcgtccag cttcatatta tggaacgctt
    18421 cagactccca ttgatctccc agtttgcgaa atgcccaggc atggtctgaa ccggcccact
    18481 gacggaattc cttttcatga catgctccgc actgcatgga aataggtacc tcattctggt
    18541 cccagtgctc actgcctcct gaaaaaacaa ccggctgcgc cgtcgctcca aaacgtctgt
    18601 ccatccaagc cttcatcatt gtgcccagtc cgatcaaagc cccaagaaca acgaggagaa
    18661 ccacccactt cttcatggaa gggcggttca caggggcatc agcagattca ggggcgggca
    18721 tggcggaaat attctgccat atcatacgcg ctccaccaag gaacttttac atcaggtagc
    18781 atagtccttg acatgcccag tacgtaaacg gcagattatt cctcctcttc caggacctcc
    18841 tcccgcatca ggggagtaat ccgagtcacg gtacagcgct ccacaccgcc attctccgtc
    18901 cgggtcacct tgaattccag ccctctatag ccgacagtat ctccttcctc cgcaggagct
    18961 tccagcaaat taagaatcag gccccctacg gtgtctattt ccccgtcctc caaatccata
    19021 tccatggcct cccccaattc ctcaatgcgt gccagaccgg atacgtcaca ggagaagccc
    19081 accgcatccc taggcaactc cgtcagtact ttaatgcggc cgcggtccca gcccattact
    19141 tcggaaaaaa catccgtcag ggtcaaaagt ccggaagtcc ccccgtgctc atccagaata
    19201 atggcaaggc gcacattgtc cttcctcatg atttccacca cattgtcaaa cttcaccgtt
    19261 ttgggaacct tgggtatggc atgaatgtat tcaggagtca gagcctttct gcgggacatg
    19321 atacggaaca ggtcgcgggc atgcaccatg cccaccacat ggtccagatc accgcggtag
    19381 acagggtaac gcgtacggcg ggtgcgacgc acaatttctc gtatctgctc atgttccgcc
    19441 ccttctggaa tggcatctat cctgacgcgc ggagtcatga cctggtgtac atacagatca
    19501 tccaggtcaa acaagccgtc catgatgcgg ccggattcct gcggcaacgc cccattctca
    19561 tggctctctt caataatata ctggagttct tccgcagaat ggtagtggga cttggattca
    19621 ttacggtcca cacccagcaa ccccagaaag aaattaccca gggcattcat acccactacc
    19681 aacggataca ggcagagctg gatcacgtac atgggcatcg caatgatgag gcaaagcttt
    19741 gtggcgtact gtagggaaag cgccttgggc accatttccc caagaacaat atgcaggtac
    19801 gtcagcaccg ccacggacag gatgctggct gcgccatgag ccaccagcca ggaagcccag
    19861 ccgtactgcg ccaaccattc atggagccat ccagctaccg catgctctcc gtacataccc
    19921 aaccccagac tggcgaacgt gacgccaagc tgcgccgtag caatgtagcg gtcctgtaac
    19981 cggggcgttt tcaatatctg ggaaacgcgg cgcgccacca tgttgccgga cacacccatc
    20041 tgctccaatg ccgcacgcgg agcgccaagc aacgcaaatt ctgcagccac aaacaggccg
    20101 ttcagcacaa tcagcaacag aataatgaaa accgtcatca tgcatccacc ccgctttcca
    20161 tggcaaattc attttccttt ctctccggag gcagcagcag gatagaggaa acacggttat
    20221 ggtccatctt ttccaccaca agcacgcgtc cggcgataat caggcggtcc ccatccttcg
    20281 gaatgcagga catgtgcttg atcacgcagc cgcccacagt agtggcgcca ttcatcaaaa
    20341 agggtaccag acattccgcc ttatgggcac gcatcatgcc ggggatgcgc acggaaccat
    20401 ccggaaggtt ttccaccttt tccccggagc ggatgaattc ctcatctata tctcccacca
    20461 tttctcccag cagacgttcc agcgtcacca gccccacaaa cttgccgtat tcatccagaa
    20521 caaaggcttc cttggccctg tcttcccgca aacgggccat cagcatctct accgtctgca
    20581 tttccatcac gcatggcacc tgcctcagca tggaacggag aggaggcaga attccctttt
    20641 ccgcataagc ggctaccata tctttaatat gcagcatgcc aataacggat tcacggttcc
    20701 cttcatacac gggaatatgg gtatgaggcc tatctgcgat catattcaaa atatcttcct
    20761 gtgtggcatc cacatccagg caaacaagct gaaaacgggg aatcataatt tgttccaccg
    20821 tgcgttccgc cagggacaaa gcgctgtgca gccgttcatg ctcatcttct tccaacatcc
    20881 cgccctggtg gctttcatcc agcagcatat taatctcctg ctgggagtgg atgtgctgat
    20941 ggccgccggg tgggagccgg aacagtttaa gaagcaacaa accgcttcca ttaagaaagt
    21001 caatgaacca tgcgaagaaa gccagtgtcc atcgcatggg ataataagta tacaaggccg
    21061 cctgcttggg aaactgaaga gccagggatt tgggcatcaa ttctccaaga attacctgaa
    21121 aaaccgtcaa gcctatcagt acaacaagag cggctgcaga attggccatt acggagtcca
    21181 tcccaccaaa ccgctcaaac agagggaaca gataggcggc cagttcaacc tgtccgtatg
    21241 cgcccagaac caggctggaa atggtaatgc caaactggca ggccgcgatg tagcggtcca
    21301 gctcttttgt attttccaaa atcttaagca gatgaacagc caccctgctg ccgttctccg
    21361 ccatttcccg gatcatgttg cggcgtacgc tcactgcggc gaattctgct gcaacataaa
    21421 aggcgttgac cagaatcaga aaaagaataa cgaaagtgac aaaaagaatc atgtatgcag
    21481 gggtgtccgt aaaacgcaac gggcatccgc atcataggaa ccgaaaaaat cctgtcaaga
    21541 acgggaaaca aaaaaatctg ccatgcggaa agagctccat tcctcccgtc cctttccagc
    21601 attccaaact ttctccttcc ggtacaggca gaaaaaattc cgtctgatct cgcaagcagt
    21661 tgaaccaact gcaaaacatc tttccagcaa tcgaaaagcc ccgttcccgg aataaccgga
    21721 aacggggctg agaatttaaa ggctcaacga aaaacgttat tccgtaatgc cggcggcttc
    21781 tgcttcggca gccttggcat cgcgcaaagc ggcacgtatg gacattttca cgcgtccctt
    21841 gtcgtcaata ccgatgcact tggcggtcac cacgtctcca acgctcacga cgtcctccgt
    21901 cttggcggtg cggccttcgg ccagttcgga gatatgaatc aggccatcct tgccgggcag
    21961 cacttccata aacgctccaa aggtggtcgt ggaaacaatc ttaccggtgt acagttcccc
    22021 gatttcaatg gtcttgaaca tgcgcgtgac caattccaga gcacggtcca ggccttcctg
    22081 cttggcggcg taaatatgca cggtgccatc ttcctcgata ttgatatcgg cgccggattc
    22141 ggcttggata gccttaatgt tcttgccgcc ggggccgatc agttcgccaa tgcggtcagc
    22201 gggaattttg gtggtctcaa tacggggagc attcgggctc atctgggccg gagcggcaat
    22261 ggcttcattc atgaccttca gaacatccgt gcggccggct ttcgccacgt gaatggcttc
    22321 ctccaatatg gagaggggaa tgccgggcag cttgaggtcc agctggtagc ctgtcacgcc
    22381 ttccgacgtg ccgcaaagtt tgaagtccat atcaccgtaa aagtcttcgg aaccgatgat
    22441 atccagcaga gttttgtaag aggtgatttc atgctggtca ttctgttccg tcaccaggcc
    22501 cacggaaata cccgccacag ggcgcttgag gggcacgccg gccgccagca gagacatcgt
    22561 gcccgcacag acggaagcca tggaggtgga accgttggat tccatgattt cagaagagac
    22621 gcgaatggcg tacgggaatt cctgttcgcc aggcacgacc ggagcgatgg aacgttccgc
    22681 caaagcgccg tggccgattt cccggcggtt ctgaccgccg aaacggccgg tgtctcccac
    22741 agagaaaggc gggaaattat agtgcaggat gaaacgcttt tcattcacgc tgcccgtgta
    22801 attgtccatg tactgacgtt cttccatcgg agcgagcgtc gccaggcaaa gggacatcgt
    22861 ttcaccgcgg gcgaacatcg cggaaccgtg caccacagga ggcagcacat tgacttccgc
    22921 agtcagaggg cgcagctgct tgagagcccg gccatcggcc cgcttgtcct tttccatgat
    22981 ggagatgcgg aaagcctttt tctgaatata ttcaaaaacc tgttccacat caaagtcagt
    23041 agcttccgga tggcgttcct tgatggcggc ctccacttcg tcacgcaggg cccccacttt
    23101 tttctgacgc tccaccttgg aaggggcgta aatggcttcc tcaatacgat cgccggcaat
    23161 ttcataaccg atttccagca attccggctt ggcaaggcaa agttcataag ctcgcttctc
    23221 cttaccgcaa acggcacgga gctcttcctg cttttcgcaa atgaccttca cgttctcctg
    23281 ggcaacacgc aaagcggcga taaaatcttc ttccggcagt tcattagcgg aaccttcaat
    23341 catgatgacc tgatccttcg tgccggcaaa taccagatcc agctgactat gttcacgctg
    23401 ggagttggtc ggattgataa cgaactgtcc gtcaatacgg cccacacgca cagcccctac
    23461 gggttcggca aagggaagat cggaaacgac acaggcggcg gaagcgccgt taatgctcag
    23521 gatgtccggt tcgttttcac catcggcgga gagcagaagc gtaattacct gcgtatcgta
    23581 gaagtagccc ttggggaaca tcgggcgcag ggggcggtcc gtcatgcggc acgtcaggat
    23641 ttccttttcc gtgggacgcc cttcgcgttt gaaatagccg ccggggaaca tacccgccgc
    23701 agcggccttt tccttgtact ccacggacag ggggaagaag gtttgtcctt ccttgacttt
    23761 ggtggcgctt accacggtca caagaacgac ggtatcgccg ctgcggacaa caacagcgcc
    23821 gtccgccaaa cgggccatct tgccggtttc aatcgtgata ggattcgtac caacgttgca
    23881 ttcaactgaa tgtatgctca ttatattttt tgtctttcaa tcacccgcag cctcatcagc
    23941 ccatccaggc cggctccggg cgggaagcct gaatggcgtc aggtacgggc agtgcctcag
    24001 gaagatgtta tccgcaacag tcctttccca gaaacagagg gagaatactg tccacgcggc
    24061 aggacgcaca tcccagatgg atcaaacgct ccggcccagt cttaccggct tgacagcctg
    24121 tgaaaatccg aaaatttctc aaggcatgcc cgacagaaac gtttcgggaa acgaatcccc
    24181 gaacgtcgaa tgagagcata atccctgtca aggataatgc aagcaaatag tttgataacc
    24241 atagcagtcc atttccttcc agggctttaa atatccaccc gcatcgggag aaaaggtatt
    24301 tttcccatct caccttcatc cattcaatat tcttcgcatt cctgattgaa atgcattact
    24361 ccaagcaaaa tatccggaaa cgtccgcctt cccttcttca caggagcgga aatgcagaat
    24421 ttaaggacgg actgagtatt cctgcctgac tcccataaca gccgcccact gctttttcat
    24481 ccatattttc ctattctttg cgcggctgca tcatacccgc aataccggac tttccgtccc
    24541 cagcaaacat ttacgcagga ataaaggggc agtcatgcca acaaaaacaa tctccacact
    24601 ccagaatatc ttctttcaca aaaagacagg cctttcaaac tctcctgacc aaaacacaaa
    24661 tccacagtca cccgccatgc cccccttcca tgacaacgct accatgccag cccctcccat
    24721 aaaaacaacg gccgctgcgg aaaaccgcaa cggccgaatc aaacgggaat atgctccgcc
    24781 ttttagcggc gaaggccaag acgctggatc agactctggt actgggccag atcttcacgc
    24841 ttgacgtaat caagaagctt gcggcgcaga gccaccatct tcaacaaccc gcggcgggaa
    24901 gaaacatcat gtttattcgt gctcaggtgt tcggtcaggt gggcgatgcg ctgggtcagg
    24961 cgggcaatct ggtagctgga actaccggaa tccttttcat gcatcttgaa ttcctgcaaa
    25021 ttgatttcgt tactcataag tgttttcctt gaatgtattt agctcgtggc agcggctaat
    25081 cgacactgtg agggcgccaa ctatgcatca tccagcccca aaagcaattc aaatttcgtc
    25141 atcccgggga atttgccttc ggtccctggg aaagcggccc ctctccattt tccccggtgt
    25201 cccagacttc gcggtccttt tccgtcacgg ggcgaaccac acggcaggga ttacccacgg
    25261 ccactacatt ggcggggatg tccttcgtca ccacgctgcc cgcgcctatc acggaatttc
    25321 tcccaatacg cacgcccggg gcaatcaccg catgaccgcc tatccatact ccatcctcaa
    25381 tggtaatggg cagagcaaat tccagcccct gccgtctcag ccccgagtct acgggatgcc
    25441 cggcagtata aatgcccacg ttgggaccta tcagcacgtc attgccgatg gtaacggcag
    25501 cgcagtccag caccacgagg ttgaaattga catacacgcg gtcccccagc cgtatattgg
    25561 agccgtaatc acaacgcagc ggaggttcca gataacaccc ctctcccatt tggccgaaaa
    25621 gctcccgggc caattcccgg cgcaggcttt cattttccgg agcggactgg ttataacgga
    25681 aaacaagccg gcgcgccctg agccgggcgg cagccaattc agaatcctgg gcatgatata
    25741 aacgcccgga cagcatgtta ttccagtcct tcgaggccat tggatgaaga gggggtcaca
    25801 gcgtccgcct cagaaaaagg cagctgcccg tttttctgtt ctccaacaga cggcatggcg
    25861 gcggcatctt tttttacgga cgtttccgat tttacaagtt ccttctttcc agaggaagct
    25921 gaatttgcgc cagccaccgg ggcagcaggc ttgtgcaccg tccggaacat tccggacggc
    25981 ttttgttgaa gctcgtgaat attccagttt ccatccctga caaccacacg ggttcctacg
    26041 ggagctattt caaagagatt tttcgcaatc aagtggggaa cacggataca tccatgggag
    26101 gatggatgac cgggcaagta cccttcatga attcctacag ccccgttcac tctcatgaaa
    26161 tagggcatct tggcaggttc aaacctctcg ccgggacggg gggaatccct tcgtatatcc
    26221 ccatcccgca attctccgtc agcgttaata aacgcaccgt aataggaatg atggtcttcc
    26281 tccttggaag caatgaggaa gtagccggag ggcgtcatgc cggaacttct tccggaactg
    26341 atgggggcat acgcaatgcg gtgcgtcccc cggtagatat acatcatctg ctttttctta
    26401 tccactacga ccagcagctt tccgggcagc gacggatcat cttcccactt gagcaggtgc
    26461 cccggaatag ttcccgtttc ttcaaaatac cccgtcattt cttctaccgg aatatgggaa
    26521 gaggggacag gggaaagctc ccttcccgtg gtaccgcagg acgaaaacaa aaacggaaca
    26581 cacaggcagg aaaccaacgg atggaaaagg gataatttca tctcaataag tcgcatcact
    26641 gcgttagaca gctggaggcc ggaaaaaaga accgcttttt gacaaaactg tcaactatca
    26701 atcaaaaaaa tctcttttcc tttactcctg ccctctgtta ccacatccgg gcgcgcctgc
    26761 cgcgctcccg gccgttcccg gttccgtgga gcaggccatg ggaccaagcc tccgcaaagg
    26821 ttcgtgcggc atccgccgta tgggaatgcc cgtcatgcac ggggctttcc gcaatggctc
    26881 cgcctacgcc gggaggcctg gacctgtaca actccagatg ctccacgcct cccggctcct
    26941 cttccccaca gaagcctcgc gccttttcct gggtgcgggc atggaaaaaa ctgcgctcca
    27001 gcaattccct cagcgtatta atgccacgcc agacatccgt ggttctgggg acgacgcgca
    27061 cattcaccaa tcccagcctc cccatattct ccacataaga aatgccgtgc gcatccctcc
    27121 gtgcagcatc atgaggcaaa agatggaatg ttaccgccac accatgattt ttctcccagt
    27181 ccttcatttt ttcaacgtaa tgggccaggg gctgctggct ggcggcataa tggtccagcc
    27241 agtggaaact gtctcccatg atctgcacaa gccaaatggc cgtatggtca ctcagcccca
    27301 gatcccatgc cgtgaaagtg ggagcgtccg gatcagcctc aaactccacc cctacccgcc
    27361 cccgctcccg cagggccatg atacggctgc cgtaaatact gccttcttcc cctgtagcaa
    27421 aagcctcctg cggcgttccc ggatactcct gcttcatgga ggcgcccatc acccgggcca
    27481 tccgggcata ccatctcttc tgccccgcat ccagaaccac tccggtctcc cgttccagag
    27541 aaaggaaata ctcatccagt tccctgctcc atctgccgcg ccccggcaaa gtgtactcat
    27601 cctggtcaaa ccagctgaaa aaaaagaatc tgaaatccaa gggggaaagc tcgcttttgc
    27661 ccatgttttc catggcctgg cgcgtcagtt cataattcac tccgtaccgt ccgccctcat
    27721 gagtgctttc cttcagaata aaacctccgg ggggaactgt attgattgcc cccgtacgga
    27781 tttccctggc tctccacgga gcatgcacgg aaacatacgc cagctcggaa acatggagaa
    27841 actgcatggt tccccctcgc agattagtcc ccacccgcac atcgctccca ttggagaaag
    27901 ccaggcggct gcgcgccaac gtacggggtt gccattcccc ctttttttcc acgccggaca
    27961 ggctcttgat tctctctccc agccgggcca gggccacatc catcgcagag gggttgggcg
    28021 gcacgtaatc cagatggtcc caggcaaaat gcagcttggc cagtttctgc tgggcgtccg
    28081 gcaggctttt atcaatgatt ccgcaatgaa aattggaccg gaacaggctc atatccagca
    28141 tcaacatggc cacatacgta gaaatgccca actggcgcgc cttcaggatg tcattcctgt
    28201 accacagccg ctcatgcagc cgccgctgcg ccctgttcag ggaaaaacgc tggagctgac
    28261 cggctttatt ttctatccag tacaggtgtt ccagcctcca aagctggctg gacaggacgg
    28321 actgccacga caggctccat gattccgtca aatgattaga attttccata ctcctctatt
    28381 aattctttca acagggctcc ttccctggtc atccccttct gggcctccgt ccccttgtcc
    28441 aggggaaggc ggtatgccag tggatcgttc agcacgtggg aaacgaaccg gagtgcctgc
    28501 accatcacgg gatcaagcag atcaagtttt cctttcaaat aactctcacg tacgcccagt
    28561 tcttcataca tcctgattac atcctccatg aagcatccca cagcctgtct tctgttccac
    28621 caccatcgtt cctgagcatc cgcatccccc agcgtttcca aaatccgacc caggaaccgg
    28681 gtacggctcg cccgtgaatc ttccggggaa ggttcctctc cggctcccgt aaaacactcc
    28741 agcggaatat aggaatcccg ttcggacacc gcaggcgctc cccggctgtc cagataaacg
    28801 ccgttccatg catgtaccac gtagggcgcg cgtgcctgcc gtacgggact ttccggaatg
    28861 cccggcatca ggcgcacggt atgccccggg tcgtccgggt cacggtaaat cacgcagttg
    28921 gcatacatcg cgcgcgcttc ctccggctca ggaagaaagg gaagcatgcc gcctctcagt
    28981 tcctcctgcc tgcggtccag aacaccaatc agggaaatgg cctcatccaa cgggagttcc
    29041 cggttccaga cagacgtcac ccccccggga gccgctccgg aatcaatccg gtaaatcttc
    29101 cctttccaac taaccccgga ttccgtagct tccggtcggc ctgcaaaatc ccgcctcacc
    29161 gcatccagcg tctccacagc ccggaccaca tccggacggc tacgccagac ggcaggggcg
    29221 ggaacgccac gcctgacgga aaaatcttct tccacataca ggggcgcttc cagaatgggt
    29281 tccggaagat gttctcccaa agcctccagg gaaacatcct tcgcgcgcag aggtcccaat
    29341 ccggtccgga acggcttttt caccaaatgc aggtacaggc cgggatgttc cggatccgga
    29401 cgccaggaat tcagctgggg gaactcctga agcgcccggg acagttccct catgcggctt
    29461 tccgccagcc gctccatcct gcgtacagaa ccgcccgcgt atccgtccag cgcggacagg
    29521 gaacgcacca gtccctcttt cacgtgatcc ggaacggtaa acaacagccg tccggacatc
    29581 aggctgtgag agtaccgctc caccatgggg aggcggacct cccctccggc cggatcccag
    29641 gcacgctccg cccgtatgcg ttcattcata ccaaccgtct ctcggagcat tcccataaac
    29701 tcggcagtgt ccgcatcctc cctcagcgtt ttcttcagca atccggcccg gcgcaccaat
    29761 tcctcaagag gttccggaac cctgtcaggc attgcggccc cgtaacgcgc ccaggaagcc
    29821 accgtttccg gtacggtgtc atgatccaga tgggcaaaaa aatactcctt ggacagctgg
    29881 gacaactctt caatcagttc cgtttcccct gggagcttcc tgcggcgcgg cacacgttcc
    29941 aaatgtttca acatctccca agcgccttcc ggggaaagct gatccgtgcg catcagccgg
    30001 tcccatacga cttccgcctt gtcttcgatc aaagccaggg gattggccgt attatgaagc
    30061 ctcagccggt gcattaccaa ccccttggct gaggaaacgg gcaaattatc cgcatccacg
    30121 cccagcctct gtacggaacc cacatacttt tcgtgaatat tcatgcgctc caacagggca
    30181 tcacggctca tcagcgtttc agcggcaagg cgcccccggc ccgtcacctc cagacgatcc
    30241 ccggacccca ccgctccgcg cacgccgtaa ccagctttca gcatatccgc caccgccatg
    30301 gccgtcaggc tgtcatacag gcaggggagc cgccccccct gcacgttttc caacccggtg
    30361 gtcagcctca gataattggt atcccattcc ggccgcccgt tatgggcatg ccattcccag
    30421 ctacggatca aatccctctt agccgagcag gcggcagaaa acatcatgga aacattggcc
    30481 atcaggtccg cttccgccgc ctcccgagaa ggatgccagt ccgaccacct tccattggga
    30541 tactttactc tccacagcct ttccgcccct tcccctccgg gtgaggactg gaacaggcgc
    30601 ggggtcagca ggagaagact ctccaccgct ttccgcgtcc ccggataaat acgcggcgaa
    30661 acctgttccg gaccggcatg cagccgcgga gcatccagca cagaggcata acgggccacg
    30721 ttctccggag ccacctccag aaaccgggcc agcaaccgcc cgtaactctc cacgggcgta
    30781 tagccgcgcc ccacatgcac gtcaaattcc ttcatgttgg gaagtacgtg gtacaggctg
    30841 ttcacatccg cctgggttcc ccataccatg cggttcatct cccgcagctc agggcttacc
    30901 ccatggcgtt cctctccgcc aagagaagcc atcatgccgc tcatcctggc cagggcggag
    30961 ggaaggccgt ccggattaaa agattcagac aacaaatccg gagcattcag caacgcactc
    31021 ccttcctcca gccaccggcg cgccctgccg caggcgcctt catcccggca aaacgacttc
    31081 cataaacgaa aagccacatg cccggacgcc tcttcatgcg gcacgccgtg tacccgttcc
    31141 atcactcctt cataaacatt ggctcgtgtc cgcagatcca tatcctccgt aacggcgtcc
    31201 cagtccctgc cggaaaaacc cgcacggcgt cccgcctctt ccggataagc cagcagcaac
    31261 atcctgtacg gccgatagga gcgcttcttc agcctgtccg ccacaaacgc tcggcgcaaa
    31321 gcatccgcat gaatatcata ttcctccgca ttccttaaac gccatgggat ttcccgtccg
    31381 ttcctgttcc ggaccagacc caaatcttcc tcttccatac ggcccagagt ctcataccag
    31441 gaagaaagag cctcctctac ggaaggccgg ccctgcgttc gcttcatctc cacaccggga
    31501 gccggttccg ccgcataaac aagctgcccg tccgctgcgg cggcttcacc cacgcggggc
    31561 agaccggcac gcaacagcca cttggttctg gccgtaacat aatccggatg aggaaaaagc
    31621 tttttcaaca ccgcttccga ttcggggaaa tcccgcatct tccaaggctt tgtacgtaca
    31681 ggagaaggca aatccaaaaa atctctcaca tcctgttccg tcttcaaaaa aggctctccg
    31741 gcttcatcca gggcgcgaga ccactccaaa gccttcctag aaatgtacaa agacccccac
    31801 agcggagaat tacgcaggga cttctgcaac aattccccgg cacggtttac atccttatcc
    31861 tgcaaaatcc tggaaaccgt ttcatccgga attccgaata ctttcagagg ggaaccgtct
    31921 ccaagcagca ttccggcatg gcggaaatgg ttcagagcga cctttcccgc gcctatcagc
    31981 aggaaaggca tcatcatgac ggcctccctc aactggtcat ccgcggaagc ctgtctggcc
    32041 ttccatccct cccagtccac accggacgcc ttattcgtaa gagctcccac gccttcctga
    32101 acgaccatgg gcgtcaactc catcaattta ttttccgcct ccatcttcac ggcatccgcc
    32161 gcaacggctc ccattccggg aaaaaacgca ccaggattcc ctgatgcggg cctcatggcc
    32221 cggaacgccc gcattccctg ccccaacatc ttctggccca gtttcgtcat tccgaaggaa
    32281 agcaaggtat tcaccccgcc ggagacgaca gctcccccca tctgggcatc aaacacgccg
    32341 tccgggttca tgctccgggc ctccgccata tggcgccccg tctcccccgc catcaacatg
    32401 ccaagcccgg cagggccgca aaacgccaaa gccgtggacg ctccctgctg ggccatgtcc
    32461 accagcatct cccggaacca gggggaatcc tttcccccac gcagcggggc aaactccatc
    32521 ctggcaaaat tcctcagttc ttcaaaactc cgggaataat tgtccagcgc ctccttttcc
    32581 cgtccctccg gcaggagcca tgctgcgccg tttacggcca gctgggccgc attctccgca
    32641 acctctgccg caccgcgttt aagcgccccc cccagggcat cccgaaaggc ggggcgggaa
    32701 cgcaaatagg gagccatcat gcggaacacc ttctgacggt ctccatcctc cagggaagcc
    32761 aggcaaccca cggcgcgtat gacgtccgga actccggcaa cggctttccg gttcagttgt
    32821 acggcgggaa aaccttcctt caccggctcc tcactggcta ccagcccctc caatccc
//
LOCUS       DAOMSU010000003        28992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_34_length_28992_cov_2.840792, whole genome shotgun sequence.
ACCESSION   DAOMSU010000003 DAOMSU010000000
VERSION     DAOMSU010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 28992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28992
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34_length_28992_cov_2.840792"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="PK077_00260"
     CDS             complement(<1..828)
                     /locus_tag="PK077_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRN22764.1"
                     /translation="MKTIIHPLAAILLAWSAGASETWSTSPAEAMQQAAAQNKGVMLE
                     FTGSDWCGACIMQKKQALSLPEIQTAISRSFIPVELDYPRKKQQDAQTKTSLETYKKS
                     YGITGFPTLVFADAQGRPVHTVVGYANPAQVMQDTKKAAEALNTQQSLTNKLAEKLTD
                     QQRRDTLVQLLKTVPQSSIRTFYKPALAELEKLDPQDASGILAKLHRDDLLHAQKLEW
                     TDTFRKKNVHILADQNPDEALSIMDSYLKKNGLLPEVKQAVLMQKVYLLIQQNRVNEL
                     "
     gene            complement(842..1846)
                     /locus_tag="PK077_00265"
     CDS             complement(842..1846)
                     /locus_tag="PK077_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22765.1"
                     /translation="MTDAPAPRQPSGETLPPAEDETRFAPKENGGEHKDENSAPAAKK
                     RLKTSLDVVSEHAAEEESARQEDSERKARGDALFWNLCLGLFALIVISAGYIVYEKFS
                     QLPDPVKDAREALEDNHAQLLKKQEQLREIRNRTAPKEQLLSLLDIFEHTASDLEETQ
                     KSIEKEKMRVAGIRGEIRSYFERYRQHARAKARGRKFDILKTSHSGKTYLNVEITRVE
                     NEFVRIMHEQGSTSIPAGDLPDDIREMLAYGDPLNITAMNQTDASLKSPVIRRAPSPA
                     PAAAPVPQPAKKKVVVEDLDPPAGKPKIETPTHTGDSGSSSSGNMWVPPAHSPLPPMQ
                     "
     gene            complement(1872..2315)
                     /locus_tag="PK077_00270"
     CDS             complement(1872..2315)
                     /locus_tag="PK077_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22766.1"
                     /translation="MFNIVRFTRRTCPALALTLALTSCDSSTNEALTSFLTGNSSQEH
                     QQLQAQLQQMKNALAQTEQEISQAESHDARVNYNLQKRLSNPGRITTPFTVKDIDVLP
                     SKRRDFEHLCFDIEDMRKTLAQRKPCNINITRMRPNWLNSNKPTR"
     gene            2424..3698
                     /locus_tag="PK077_00275"
     CDS             2424..3698
                     /locus_tag="PK077_00275"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: protein motif:HMM:NF006964.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HRN22767.1"
                     /translation="MMQLSEIGGHLTARTGIDDLMEDLFKALHSGDAGLCQLNGGSPA
                     VIPEVTELWRRSMEELVRNGKFDVLVGHYAHPGGDPAFIRALVCFLNERCGWNLTPEN
                     VAITQGGQMACFTLFNMLAGPCPDGAVREILFPLCPDYVGYQSQSLCGGVMFRGIRPG
                     IRMLDGHTFKYVIDFDRLDIRPETAAVCMSRPTNPTGNVVTDEELGLLREMTSRAGVP
                     LMIDNAYGPPLPNICFVPVTPAWDENMILTMSLSKIGLPGTRTGIVIARPEIIRAVVS
                     MVTTSSLCPNNLGQVLVTPYLEDGTLERVCRETLTPFYRGRAEFALSLLPELFGESIP
                     WRVHKSEGAMFLWLWFEGLPITCQELYERCKSRGCFVNPGHHFFFALPEVGEPWPHRH
                     ECIRISFTQTEELLRKGLSIVADEVRKAYSRS"
     gene            3719..4594
                     /locus_tag="PK077_00280"
     CDS             3719..4594
                     /locus_tag="PK077_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HRN22768.1"
                     /translation="MNSMTGFGRDAAQTDRYNILVEISGVNRKQTEIAVNVPRGYAEW
                     DASVRSIVQGAVSRGRVGVSISVERLEEADGSLQLDEQKLASLAELLNRASDLTGQPL
                     PLQASDLLRLDIVTSAAEASLSPEEAWPMVEKALKAALKDFISMRAAEGANLKADVLG
                     KLDVLEQFRLRIAEHAPSVPVRLREAMLKRLADADLSVSADDERIIREVALFADKCDI
                     SEEITRLSSHFDQFRTLCGSSAPAGRPLDFLCQEIFREFNTIGSKANDSTLAHLVVAA
                     KTELEKIREQVQNIE"
     gene            4600..5193
                     /gene="gmk"
                     /locus_tag="PK077_00285"
     CDS             4600..5193
                     /gene="gmk"
                     /locus_tag="PK077_00285"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRN22769.1"
                     /translation="MKQPLGTLLVVSGPSGSGKTTLCRRATENGLCVYSISCTTRQPR
                     PGEVNGVDYHFLTPAEFDARVERGDFLEYAEVHGNRYGTLKADILTLLEQGKNVVMDI
                     DVQGAGQVRSCREGILPLCYADVYIYVPQEELKSRLCGRQTDGAETIALRLRNAEQED
                     ACLPQYRYCLVSSDRETDYASFCALLKCQSMRVGLMR"
     gene            complement(5720..7594)
                     /locus_tag="PK077_00290"
     CDS             complement(5720..7594)
                     /locus_tag="PK077_00290"
                     /inference="COORDINATES: protein motif:HMM:NF015557.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRN22770.1"
                     /translation="MLPLLNINSASVIGWLESAAAQQWIVGILLVLLFISFIKEWMPV
                     EITALTGTAVLMLTGILSTRDVLSSFANSGPLTVVCMFILSASLERTGLIGDLSKLFN
                     KVAKGRELTALLVITLGAFMVSPFVNNTPVVVILMPIVLAFCRDHNIAASKLLIPLSY
                     ATILGGTCSVVGTSTNVVVLGQVQKLGYDGIQMFTVTPMGLIYAAAGLLYLWTLGRKW
                     LPSRPTLSTMLPGGIQRDFLLQVRIPADSPHIGTTPINLMQTELLGTKIVEVRRRGFS
                     MQEELQHINLEEGDRILFLCNARKVNQVREAKGVDLGWDDSRGLETLEQRDVQIVEGM
                     IANNSEFAGLSLSELKLRQRFNIFVLAIHRQGKNITDMGPNTKLAAGDTLLLEGPQEG
                     MNRILTKQRIIPLSQRPAEAHNRSKQGWAILAMGLFIFIGLLGSFEQYGEFFKFFARF
                     NPFYLAFIGALIVIISGCIKPKEAYQSVDWGIIFLILGMLCVGEAMSKTGLAKAIAFG
                     VVDNIGPLGCLVAISGLYLLCSILTEMISNNAVAAVMGPLAYEMALQFDANPVPFILA
                     VMFGASASFSTPIGYQTNTYVYNAGGYKFKDFVKVGLPLNLLLWVIFTCAIGWLYPLK
                     "
     gene            7726..8571
                     /gene="gluQRS"
                     /locus_tag="PK077_00295"
     CDS             7726..8571
                     /gene="gluQRS"
                     /locus_tag="PK077_00295"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HRN22771.1"
                     /translation="MDSPIITRFAPSPTGNLHLGHALAAWEARSLANRFSGKCVLRIE
                     DIDQTRCRPEFVQGILKDLEWLGIRFDGPMMVQSSRFSAYEHALQTLKDRGVLYPCFC
                     TRREIAEEVAAMGGAPQGGCVDVYPGTCRLLDKGRRKELLKSGKPFSWRLDCRAAARI
                     TGPLLWRDMRFGDQVCRPEELGDVILGRKDCAASYHIAVVVDDAAQGVTHVSRGEDLF
                     PVTGIHRTLQALLGLPVPQWYHHRLVKDASGKRLAKRDRSLSLQEMRAAGMRPEDVFR
                     LMRES"
     gene            complement(8628..9107)
                     /locus_tag="PK077_00300"
     CDS             complement(8628..9107)
                     /locus_tag="PK077_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22772.1"
                     /translation="MNHDAAPAPPPRGLLIKSAGLLTAISPLCTWLLWDTLPASALLA
                     PLFLVSIFAGTARAMKKNIHETFLPVLPAVWLTLAAGLAFTAVSLFSQAAFIWLPQIL
                     TGTAAMPHAWLIPLGHLNLLAILSSLLLLTLAAAYACLLCIGKKTTRPAAGKTQATA"
     gene            complement(9113..9544)
                     /locus_tag="PK077_00305"
     CDS             complement(9113..9544)
                     /locus_tag="PK077_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22773.1"
                     /translation="MNPLPLIPAFLILPVLFSCTQKSNLEIATGKVTTIKLGNVVRIK
                     EINVLTSTDDGTFANMAGTAAEAMTPGIIGMGTSKLASATGSIVDGRVEAVKMIRVRV
                     KLKDTNELVEVQQEVTPNFTLKIGQEVVITQGSTPGNVWPD"
     gene            complement(9585..10511)
                     /gene="menA"
                     /locus_tag="PK077_00310"
     CDS             complement(9585..10511)
                     /gene="menA"
                     /locus_tag="PK077_00310"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase"
                     /protein_id="HRN22774.1"
                     /translation="MNIITSAFLAARPKTLTASLIPVWAGCMVVQKLTGSWNASLALL
                     TLASCLCLQIACNFFNDAIDHAKHADTEQRTGPVRMTASGALSHGTVMLIGLFFLLGA
                     CLLAFPLIELRGWPILAIGIPSLYFTYGYTGGPWPLAYKGLGEIFVILFFGLVAVLGT
                     ILVQIGTAPVVPGTLVESLAVYNAGIVTGIQCGLLCAVMISVNNIRDRKEDLTTGKRT
                     LAVRLGEGKARAMAQSFILAAYITLPTSSRALHLNLSHTWWMWIPSILFGGYLMLLIR
                     KTPADSRMNRVLALSSLHLLLYLATYTILPTR"
     gene            complement(10531..11238)
                     /locus_tag="PK077_00315"
     CDS             complement(10531..11238)
                     /locus_tag="PK077_00315"
                     /inference="COORDINATES: protein motif:HMM:NF013640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HRN22775.1"
                     /translation="MQQIVREHQAYPPKIVASVTSWELWKTLKGKDLTDQCDLVELRV
                     DALPPELTTEQVMEFRPEMPLLVTVRCHEEGGLRRIPEEERFALLRAYMPYAAAMDIE
                     IKAMRHARELIMEARDRDVIIIGSTHDFQITPGVDYLREQEKKARSYKADIVKFAFTP
                     CLASDIQTGVQLFTKPKGPIAVMGMGPMGPVSRLLYSQLGSCLVYGYLGEEETAPGQW
                     HVSLIKETLRRLGPILR"
     gene            11598..12392
                     /locus_tag="PK077_00320"
     CDS             11598..12392
                     /locus_tag="PK077_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22776.1"
                     /translation="MGRNPFWAALDAVLVVRTEKDSSGHPEWSWKAYVPHGVPVHELP
                     PVPVPLPEGDWDKERFRKERRKAARVLGHQAALTLASSLHFSRFMIVEEECRFRLPVI
                     GSTARMLGQLLKGCRERTALFLSEPRLSPHTACSVAKGEKSILLRVTDMAASSAYVMD
                     LSLCERILLSLPSDQRVWPWLRYYGSLEEWFARYLGRYGDLYALSPAFVSCGEGAPIP
                     SLCKSLGHFNNARTKYTDLSYLVNPAVNSWRWLKNVNIFSGRKRLG"
     gene            complement(12599..13519)
                     /locus_tag="PK077_00325"
     CDS             complement(12599..13519)
                     /locus_tag="PK077_00325"
                     /inference="COORDINATES: protein motif:HMM:NF040375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HRN22777.1"
                     /translation="MAVTICDNYQVKYTRKWGTLLQQHASRLDKYVSVMRDLSGKVVF
                     LDQFGILDFTEKTTRVGQTVLNEAPTTRRSMRPRTFTKAIGYDEFDATRLGDMDLPVS
                     KTIEGLQAAAGRRMDDVMISGFLDTNYVGEDGMTAVPFKESQQIAVDHVDSGTKSASN
                     LTVAKLRAALQLFEENEAWNQDAPQFGDQLVIAVTSSQIMNLLRETEVSSYDFNNVKA
                     LVEGKIDTFMGFKFIRTQRLPKTEEGVRSCLAWVKSKAQFGIWNDFKVKLSVRDDMEE
                     ALQIRAKFACGATRLQEEGFVKILCDEGAS"
     gene            complement(13745..14416)
                     /locus_tag="PK077_00330"
     CDS             complement(13745..14416)
                     /locus_tag="PK077_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HRN22778.1"
                     /translation="MKPIQSYPGEIVQFIERIPGVDVAGDRETVLERLEPYHKAEVEA
                     LGWRWKDLRRAEQVHGTKVALVGDIGSNYPVEGADGLICSGVSDCVLAIYVADCAAVW
                     LYDKENCHRSLIHSGKAGIAGNIVSNTIKSMKKCLGVEPSNLIAVIPPCIRPPHYEED
                     IPTAIKAQLIEAGVPEEQIHDSGLDTAADTKRFYSYRMEKGQTGRMLALFGPDHATRT
                     HAIIL"
     gene            14632..15312
                     /locus_tag="PK077_00335"
     CDS             14632..15312
                     /locus_tag="PK077_00335"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HRN22779.1"
                     /translation="MFFSFAASLAQEASPDAGQLLKRVRQGATLQENKDIKGQIRKRS
                     VKIPFSMSLRGNLIAFQYQLNNVWNRFDLKFKDRGQEILSWKDGKAGVLPVAQYAVPI
                     AGTDVTYEDLSMRYLYWPQAKIVRDDAASTVKGRDCWIVQIPNPNSGVGQYAWVRVWI
                     DKENGAMWQVDGIDRRGELAKRFMIDSVMKLKDGSWFFKRMKVEVRDPSNPRRTVSVS
                     YIDMDSPE"
     gene            15459..16421
                     /locus_tag="PK077_00340"
     CDS             15459..16421
                     /locus_tag="PK077_00340"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRN22780.1"
                     /translation="MKKHLFLACALAFGMSACSQHQEQPAESGSPVKTTISPVTAPGK
                     KPDRPAVKLPPKSVPVPSVSPSYNNTPLSPRIPGVTVNRVAVPDKVVALTFDDGPHGT
                     LTPRVLDILRDNNVKGTFFMQGCNVKAHPQVVRRMVNEGHEVGNHTWNHVYLSKVPRE
                     KAESQLQSTNEAIRNACGVVPVVMRPPGGYTNAGVASWARQRFGFTTVMWDVDTNDWR
                     KPGSSVVAARAVNGAKPGSIILVHDIHASTVAAVDAIVKGLKNRGYELVTVSELIRRG
                     RAAAGHAPSMPAAPAVPDSSPLAVPSIVAPAEPVPAPAETVAGM"
     gene            16670..19051
                     /locus_tag="PK077_00345"
     CDS             16670..19051
                     /locus_tag="PK077_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HRN22781.1"
                     /translation="MSAPLAFIYDLFNNPLFIFIGGLVLLGVFFWYLGSDQDKVKRNA
                     GTIFIIGIASFSLFSLFQQGLKYGIDIAGGVSFTLSVEPNIGDDGKPIPLTDQAMEQA
                     CVILTDRLNSTGANDVIIRPKKKDNLNLIEVQIPAADPAKREETKAILTKVAKLQILP
                     VHPRNNELVAEGRIRVPGYRMYEYTFKNGKGEDVTEKIFLEKRESLTGRDIVRAGVDL
                     ARPGHVNVTLSNEGADKMYNLTSRMQLGHDRMAIVLDDVVKSAPTVQAILSKSFEISG
                     LDAPGEAEALTKVLSNPLTNKLNFESASEISASLGHTALLQGEYAGITGLILCFIMMI
                     IYYRFAGLVAIMGLTINALLLLGAMSIFGFELTLPGIAGIVLTLGVAVDSNVLIYERL
                     REEKEMGRPFRVAIRNSFDKAFSAIFDSNITSLITAVILYWMATGTIKGFAVTLTVGV
                     ITSMIGAILVTRVLFYWADTVGMMKDLKFLNLFKKKTNIDFMSQKIWSCSLSAILLMI
                     CIVFGAVKGKDCLGMDFTGGSSISYLVNKGDISFREVEGVVNKLSLTQKATVQEVAES
                     TDSSKVNILINFSDHAQDKEAITTALDKAFPVLDNAPFSEETIGQSMGYDTLITSAWA
                     LFFGILGIMVYLTVRFEWTFAIGAVIALTHDVLLVLGLVIVSGTELNVIHIGALLTVA
                     GYSINDKIIVFDRIREFLRFSDPNESASQIMNEAINQTLSRTILTSLSTLAVLVCLYF
                     FGGPSMEDFAWTISAGILIGTYSSIFIASPAALFFSRKHGLHEEVRQAMKAGA"
     gene            complement(19338..20474)
                     /locus_tag="PK077_00350"
     CDS             complement(19338..20474)
                     /locus_tag="PK077_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRN22782.1"
                     /translation="MTKQMIIAAAFAACSAFAQDAPPPPAPEAPSDAPEMQPPPERRG
                     GHRHHAGGRDRQGPKRVQMKRIDPGIMIVGEELVLAKYDTDKDGKLSDEEIAVLQADV
                     KKAQEAKKAAILKKFDKDGDGKLSKEERKAMQEEWLKDHPEAAKRMEEMKARQEARKA
                     EILKKYDKDGDGKLSDEEKKAMREDWAKNHPEAAKRMEEMKARQEAHAAAMIKKFDKD
                     GDGKLSPEELQSIPPHHGQPGPHHAVPGGPKGPRMPNMDGPRRPGGPRAEGPRAPRGP
                     EGRRGGEGRREDGNRGPHGNRPNGPRPQLDPNRAPIMAAGWILIEEKYDADKDGKLNE
                     AEMAQLNADASKALEARREARKAARGNNDMPPPPAPAQEEGDDE"
     assembly_gap    20605..20614
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(20758..21873)
                     /locus_tag="PK077_00355"
     CDS             complement(20758..21873)
                     /locus_tag="PK077_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRN22783.1"
                     /translation="MPDAFPAWVIPVVFGLMGACIGSFLNVVIYRMPRGVSVNDPARS
                     FCPECGKPIPWFLNIPVLSWLALRGKSACCGTRISFRYCFVELLTALLFAAIGWKYAD
                     ASTGAAVLLCLWTAMAIVIMFIDAEHLIVFRSQAFIGALAGTGACALYPFLLPDSHVM
                     TWTDAFTASVLGGAAGYAVIRLVIELGKLLFGTWKQHFDVPAPWSLREPESEREELQL
                     IVNGQPHDWSMLFHRSTDKAVLSGGSVTIDGKTHPACSVTLRYDRIEMENGDVFLLER
                     LESVEGNLTDIHASREAMGSGDAWILMMAGCACGWQGALFSLFLGSLLGIVQAAASRM
                     GFGRNLPFGPALLSAALIWLLGGSQWWQAYIRFISGE"
     gene            complement(21866..22990)
                     /locus_tag="PK077_00360"
     CDS             complement(21866..22990)
                     /locus_tag="PK077_00360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRN22784.1"
                     /translation="MKHPDFLDNRDFTGEDKKRPSTMSMDSSYQALEGAVKKLSEIAS
                     TRHDPRYLQEYIKTGINMAQSAASDHDFTVLIRSGREMYRANCVFAPYRHIRKISVFG
                     SARIRNDEPAYETAREFAREASEHGYMVITGGGPGIMQAANEGAGEQRSFGLNITLPY
                     EQTSNHVVAHSDKLINFYYFFVRKLNFVAESDAMVAFPGGFGTMDEVFETLTLIQTGK
                     ATIYPIVLLDSPGKTFWLNWLAFIRVELVDSGLISADDLHLIHVTKNPAEAMEHIDRF
                     YRIFHSYRFVGDSIVIRLNAQLPAQWVEHLERDFSDLILPGGKMIQSGPLPDEADEPH
                     LDRLPRLVFPIKRGNYGRLRLLIDRINQTPSRTYSPPTHA"
     gene            23200..24324
                     /locus_tag="PK077_00365"
     CDS             23200..24324
                     /locus_tag="PK077_00365"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRN22785.1"
                     /translation="MSENETTTAEETAVTTLARFEVPSRLEKIEDPNDANHLTFVAEP
                     FENGYGHTLGNSLRRVLLGSLEGAAITSVRIAGAQHEFSSLPGVVEDVTEIVLNLKKV
                     KFKHNGKEPRLLSLRVHKQGVVTAADIADDTVYQVVNPDQIICTLDQDTMFECEFQVR
                     VGRGFATGDENKVPDMPIGVIPIDSIFSPVTRVKYSVQNTRVGQMTDYDKLILEVWTD
                     GRIAPADAMLQASAILRHHLDVFVNYDDKQVNFEEAPSAVQDEETARLRKLLNMSVNE
                     IELSVRAANCLNNANITTVGQLAMKSESEMLKYRNFGKKSLNEIKDKLLELGLSLGVQ
                     VDPSLLTGPVPQAKPRLGLEEESPVGLADLIAANIEDDDD"
     gene            24361..24771
                     /gene="rplQ"
                     /locus_tag="PK077_00370"
     CDS             24361..24771
                     /gene="rplQ"
                     /locus_tag="PK077_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRN22786.1"
                     /translation="MRHGVKTSKLQRNASHRRALLANQACSLILNGRITTTLAKAKAL
                     RPYVEKLITLAKRGDVHSRRLATATIHNTTAVKRLFDEIAPLCAERKGGYTRIVKLGQ
                     RLTDSALVAMIEIIDLPREAAEKEEAPATTEATA"
     gene            24916..25842
                     /gene="fabD"
                     /locus_tag="PK077_00375"
     CDS             24916..25842
                     /gene="fabD"
                     /locus_tag="PK077_00375"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HRN22787.1"
                     /translation="MDAVLLFSGQGAQKVGMGKDLYDQYPVAKALIEQADKALGESLS
                     AVMFEGPGEELTRTCNCQPALYVHGLACLAVLKELVPSLNPVAAAGLSLGEFTAHAAA
                     GTYSFEEGLRLVQKRGAFMEEACNATKGTMAAMIGGTDENVIKLAQECDVDVANFNCP
                     GQTVVSGTEEGVAKAVAGAKAAGCKIAKKLNVAGAYHSRLMHSAMVKLAEELKNISFG
                     TPSMPVYCNYEARVVEGPDDIRSMLEHQVCGSVRWTASMQKLVDQGHRLFIELGPGKT
                     LAGMMARICKEATVISIEDVPSLEAAVAELNK"
     gene            complement(26307..27002)
                     /locus_tag="PK077_00380"
     CDS             complement(26307..27002)
                     /locus_tag="PK077_00380"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRN22788.1"
                     /translation="MTIPDHFADSREAVLKARELLRRAHAVIFDFDGLLVDTEYAIYS
                     SWERVFASCGHPLPLDLFNQCLGSGYTHWNPGEHLEKLTGRTFDWETVNSRRQEEIVR
                     DLEHAGLLPGAGELIRNLGEAGTPMGVASSSSHRWVDGWLNRLGIMPYFQTVVCRDDG
                     LPVKPDPALFLKAAENLGKSPSECLVLEDSQNGTTAAHRAGMPVISVPNRVTEQADFS
                     LATSIIRSLTELL"
     gene            complement(26989..28383)
                     /locus_tag="PK077_00385"
     CDS             complement(26989..28383)
                     /locus_tag="PK077_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRN22789.1"
                     /translation="MAIQARHFRQWSAFLLLLALGGAAAWFLMPREWSQMQWEIPGTP
                     SEYPLSQLLRPWLILLGCFLPALGMFLYNRSSILDKYVARTWFTAFIMCTAILTLIYI
                     IGDFADNVGDLMNLDAPLMGTFRFYLSQLPMILNLILPYTLLLGTLWALTKLSSSSEI
                     TGMLQSGRSLLRINTPIIIGAVFASIYFGIFGFHWAPNSALYRKLMFSSLSQNKNNHA
                     SQSTIYKNDAESRIWYLGNPPGIDSPGNPFRQVRVEQFSAPGKMKYELFADEATWDPA
                     SRTWTFRQAIKRNYSQEEPRQLHDVPVFAGQEYQTLKEHYPETPWQLISPNVRVDTQG
                     TPALQELIKSGTTNARYLRSLQTEWHVRIARIFSCIILTFIAIPSAITFQRRSAMSGI
                     GIALFLAAAMLFLYEFFPTLASAGYLPTWLGAWMPNIIYTIIAIRLFQTKLAHRSFSE
                     LLRGLKKTPVHDHS"
     gene            28656..>28992
                     /locus_tag="PK077_00390"
     CDS             28656..>28992
                     /locus_tag="PK077_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22790.1"
                     /translation="MFLLFFLAAAGVLAWFAGYVSTPLLIPVGGEEPASSPVAGSLVM
                     EPVEAVSSDGTAIEGYLVSPGRPEALSPRQSRVRDTLKLRGKMPHLEAKPELVVIAAS
                     WNEGVESSLP"
BASE COUNT         5903 a         7674 c         8622 g         6783 t
ORIGIN      
        1 caattcattg accctgtttt gctgtatgag caggtacact ttttgcatga gaacagcttg
       61 tttgacttcc ggaagaagcc cgttcttttt caagtagctg tccatgatgg aaagtgcttc
      121 atcaggattt tgatctgcca gaatgtggac gtttttcttc cggaacgtat cggtccattc
      181 caatttctgg gcatggagca gatcgtccct gtgcagtttg gcgagaattc cggaagcgtc
      241 ctgcggatcc agtttttcca gttctgccag agccggtttg tagaaagtgc ggatgctgga
      301 ttggggaacc gttttcagca attgaaccag cgtgtcccgc cgctgctggt cagtgagctt
      361 ttccgccagc ttgtttgtca gggactgctg cgtgttgagt gcttccgccg ctttttttgt
      421 atcctgcatg acctgggcag gattggcgta acctacgacg gtgtggacgg gccgcccctg
      481 ggcatccgca aacaccagcg tggggaatcc ggtgatgccg taggattttt tataggtttc
      541 cagagaagtt tttgtctgcg cgtcctgctg ctttttacgt gggtagtcca gttccacggg
      601 aataaaggaa cgggaaatgg ctgtctggat ttctggaagg gaaagagcct gctttttctg
      661 catgatgcag gctccgcacc agtcggaacc ggtgaattcc agcattactc ccttattctg
      721 tgcggcggct tgctgcatgg cttccgcagg gctggtactc catgtttcgg aggcgccggc
      781 actccaggcg aggagtatgg ctgcgagggg atgtatgatt gttttcatgg caggaatgtc
      841 attattgcat ggggggaagc ggagaatggg cggggggaac ccacatgttt ccggaagagg
      901 agctgccgga atctccggta tgggtaggtg tttcaatttt cggttttccg gcgggcgggt
      961 ccagatcttc caccaccact ttctttttgg cgggctgggg cacgggggcg gcagccgggg
     1021 ccggtgaggg cgccctgcgg ataacggggg atttgaggga agcatccgtc tggttcatgg
     1081 ccgtaatatt cagcgggtcg ccgtaggcca gcatttcccg gatgtcgtca ggaagatctc
     1141 cggcaggaat ggaggtgctt ccctgttcgt gcatgatacg cacgaattca ttttccacgc
     1201 gggtgatttc cacattcaaa taagttttgc ctgaatggga tgtcttcaga atgtcgaact
     1261 tgcggccgcg tgccttggct ctggcgtgtt ggcggtagcg ttcgaagtag gagcggattt
     1321 ctccccggat gccggctacg cgcattttct ctttctcaat ggatttttgc gtctcttcca
     1381 ggtcggaggc cgtatgttca aagatatcca aaaggctgag cagctgttcc ttgggagcgg
     1441 tcctgttccg tatttccctg agctgttcct gcttcttcag cagctgggca tgattgtcct
     1501 ccaaagcttc ccgagcatct ttgacagggt ctggaagctg ggaaaatttt tcataaacaa
     1561 tatagccggc actgatgacg ataagggcga acagccccag gcagaggttc cagaaaagag
     1621 cgtctcccct ggctttccgt tcggaatcct cctgccgggc ggattcttct tccgcagcgt
     1681 gttccgagac gacgtccagg gaggttttca gacgtttttt ggctgcgggg gcggagtttt
     1741 catccttatg ttctcctccg ttctccttcg gagcaaagcg ggtttcgtct tccgcagggg
     1801 ggagagtttc gccggacggc tgtctgggag cgggggcgtc ggtcatggga tgcgcgggaa
     1861 tggaggttag tctaccgggt gggtttgttt gaattcagcc agtttggccg catacgcgtt
     1921 atattgatat tgcagggctt gcgctgcgcc agcgttttgc gcatgtcttc aatgtcaaag
     1981 cacaggtgct cgaagtcgcg gcgtttggaa ggcaggacat ctatatcctt caccgtgaag
     2041 ggcgtcgtga ttcttcccgg gttggaaagc cttttctgaa gattgtaatt aacgcgggca
     2101 tcgtgggatt ccgcctggct tatttcctgt tccgtctggg ccagtgcgtt tttcatttgc
     2161 tggagctggg cctggagctg ctggtgttcc tgggatgaat tccccgtcag gaaggaggtc
     2221 agggcttcat tcgtactgct gtcgcacgaa gtaagggcca gcgtgagggc caatgccgga
     2281 caggtacgcc tggtgaatct cacgatgttg aacatgccgg ggattatgaa ccttaacgga
     2341 ggggaaagtc aaactgttca tgcgccctcc ggaacaatga tttttctcgc ataaaacgcg
     2401 tatgcggcta caatgcccgc ggcatgatgc aattatcgga gattggaggc catctgacgg
     2461 ccagaacggg tattgatgat ttgatggagg atttattcaa ggccctccat tcgggagacg
     2521 caggcttgtg ccagttgaac ggcgggagcc ccgccgttat tcctgaagtg acggaacttt
     2581 ggcgacgcag catggaggaa ctggtgcgaa atggcaaatt tgacgtgctg gtgggccatt
     2641 atgcccatcc gggcggggat cctgccttta tccgtgccct ggtgtgtttt ctcaatgaac
     2701 gttgcggatg gaacctgacg ccggaaaatg tggcgattac ccagggcggc cagatggcct
     2761 gctttacact gttcaacatg ctggcgggcc cgtgcccgga cggtgctgtg cgtgaaatcc
     2821 tttttcccct gtgcccggat tatgtgggat accagtccca atccctgtgc ggcggcgtca
     2881 tgttccgggg catccggccc ggcatccgca tgctggacgg gcacacgttc aagtacgtga
     2941 ttgattttga ccgtctggac atacgtccgg aaacggccgc cgtctgcatg tcccggccta
     3001 cgaatcccac cgggaacgtg gtgacggatg aggaattggg gcttctgcgg gaaatgactt
     3061 cccgcgccgg ggttcccctg atgattgaca atgcttacgg gcctccgttg cccaatattt
     3121 gttttgtgcc cgtaaccccg gcctgggatg agaacatgat cctgaccatg agcctgtcca
     3181 agatcgggtt gcccggaacg cgcaccggca ttgtcattgc gcgcccggag atcattcgtg
     3241 ccgtcgtgag catggtgacc acgtcatccc tgtgtccgaa caatctgggc caggttctgg
     3301 tgacgcccta tctggaagac ggcactctgg agagagtctg ccgtgaaacc ctcacgccgt
     3361 tttaccgtgg ccgggcggaa ttcgccctgt ccctgctgcc ggaacttttc ggagaatcca
     3421 tcccctggcg cgttcataag agtgagggcg ccatgtttct gtggctgtgg tttgagggat
     3481 tgcccatcac gtgccaggag ctttacgaac ggtgcaagtc acggggctgt tttgtaaatc
     3541 ccggccatca ctttttcttc gcccttccgg aggtggggga accctggccg caccgtcatg
     3601 aatgcatccg catcagcttc acccagacgg aggaactcct gcgcaagggg ctttccatcg
     3661 tggcggatga agtgcgcaag gcctactccc gttcttaatc caaacctgac ccgtatccat
     3721 gaacagcatg accggtttcg gcagagacgc cgcccagaca gaccgttata atatccttgt
     3781 tgaaatatca ggcgtaaacc gcaaacaaac ggaaattgcc gtcaatgtgc cgcgcggcta
     3841 tgcggaatgg gacgcctccg tgcgttccat cgtgcagggg gctgtttccc gcggccgtgt
     3901 aggcgtctcc atttctgtgg agcgtctgga ggaagcggat ggctccctcc agcttgatga
     3961 gcagaaactg gcctccctgg cggagctgct gaaccgtgcg tctgacctga caggtcaacc
     4021 gttgcccctc caggcgtccg acctgctgag gctggatatc gttacttccg cagcggaagc
     4081 atccctttct ccggaagaag cgtggccgat ggtggaaaag gcgctcaaag ccgccctgaa
     4141 ggattttatt tccatgcgtg cggcggaagg cgccaacctg aaagcggacg ttcttggcaa
     4201 gctggatgtc ttggagcaat tccgcctccg tattgcggaa catgcccctt ccgtgcctgt
     4261 caggcttcgg gaggccatgc tcaagcgctt ggctgatgcc gacctgtccg tttccgcgga
     4321 cgatgaacgc atcatccggg aagtggccct gtttgcggac aagtgcgaca tttccgagga
     4381 aattacccgt ctctcctccc attttgacca gttccgcacc ttgtgcggtt cctccgctcc
     4441 tgcgggcagg cctctggatt tcctttgcca ggaaatcttc cgggaattca acaccatcgg
     4501 ctccaaggcg aacgattcca cgctggccca tctggttgtg gctgccaaga cggagctgga
     4561 gaagatcagg gaacaggttc agaacatcga atgacagcca tgaaacagcc attgggaaca
     4621 ttgctggtgg tatccgggcc ttccggttcc gggaagacga cgttgtgccg ccgcgcgacg
     4681 gaaaacggtc tgtgcgtgta cagcatttcc tgcacgacgc gccagcccag acccggggaa
     4741 gtcaatgggg tggattacca tttcctgact cccgccgaat ttgacgccag ggtggagcgg
     4801 ggggactttc tggaatatgc ggaggtgcac ggcaaccgct acggtacgct gaaagcggat
     4861 attttgactc ttctggaaca ggggaaaaac gtggtgatgg atattgacgt gcagggggct
     4921 gggcaagtcc gttcctgccg ggagggcatt cttcccctct gctatgcgga cgtttacatt
     4981 tatgttccgc aggaggagct taaaagccgc ctttgcggac ggcagacgga tggcgctgaa
     5041 accatcgccc tgcgtcttcg caatgcggaa caggaagacg cctgcctgcc gcagtaccgg
     5101 tactgcctgg tttcctccga ccgcgaaacg gattatgctt ccttttgcgc cctgctcaaa
     5161 tgccagtcca tgcgggtggg actgatgcgg tgataaggtc ttgcctgttt atcgagaggc
     5221 tgcgttctct cgtctcgtcc cccgtcgcat ggcattcggg atggcggctc cagttccgag
     5281 acatttggaa ttggttctct tcccgtgcct atatggtatg tgattacctt tcctctgatc
     5341 cggcctacgt gaaaaaacgc gggttgaaat tcttgacgtg cagggtactc ataggctctt
     5401 tgaataattc aatgttgaaa ttatcttcca ggcaaagaac ctcaaaagaa gtgccctggt
     5461 aggtcagata ctgtcccgtt tccttgtatc cgttaattgt cttttttggc taaatttttg
     5521 actgtcagtt gaatgtggat ggatgaaact actgttttcc gttgttctct tcttaaggag
     5581 aaggataatg taaaagaaga gccctgcttt tagacgaaac agggatattg ttttttgttc
     5641 ccatgggaat gatggcgcct ttgaccagtc gctttggttt cctttccgtt gaccggggcc
     5701 ttccaaagct attatcgctc tacttgagcg gatacaacca gccgatggcg caggtaaaaa
     5761 tgacccagag gagcaggttg agggggagtc ccactttgac gaagtcctta aatttgtaac
     5821 cgcccgcatt gtacacgtaa gtgttggtct ggtagccgat aggggtggag aaactggcgc
     5881 tggcgccaaa catgacggcc agaatgaagg gaacggggtt ggcgtcgaat tgcagggcca
     5941 tttcataagc cagcggcccc atgacggcgg ctacggcatt gttggagatc atctccgtca
     6001 gaatggagca gagcagatac agaccggaga tggcgaccag gcaccccaac gggcctatgt
     6061 tatccactac gccgaacgcg atggctttgg caagcccggt tttgctcatg gcttccccca
     6121 cacacagcat tcccaggatc aggaaaataa tgccccagtc caccgactgg taggcttcct
     6181 tcggcttgat gcagccggag atgatgacga tgagagctcc gatgaaagcg agatagaagg
     6241 gattgaagcg cgcgaagaat ttgaagaatt ccccgtattg ctcaaaggac cccagcaggc
     6301 cgataaaaat gaacagcccc atggccagga tggcccagcc ctgtttgctg cggttgtgcg
     6361 cttccgcggg gcgctggctc agggggatga tgcgctgctt ggtcaggatg cggttcattc
     6421 cttcctgcgg tccttccaga agaagcgtgt cccctgccgc cagcttggta ttcggcccca
     6481 tgtccgtaat gtttttgccc tgcctgtgga tggcgagcac gaagatgttg aatcgctggc
     6541 gcagcttgag ttcggacagg gacagcccgg cgaattcgga attgttggcg atcatgcctt
     6601 ccacgatttg cacatcgcgc tgttccagcg tttccagccc gcggctgtca tcccagccca
     6661 ggtccacgcc tttggcttcc cgcacctggt tgacttttct ggcgttgcac aggaaaagaa
     6721 tgcggtctcc ttcttccagg ttgatgtgct gcaactcttc ctgcatggag aaccccctgc
     6781 ggcgcacttc cacgattttg gtgcccagca gttcggtttg catcaggttg atgggagtgg
     6841 tgccgatgtg gggggaatcc gcgggaattc tgacttggag caggaaatcg cgctggatgc
     6901 cgcccggaag catggtggac aaagtggggc gggatggaag ccatttgcgg cctaatgtcc
     6961 agaggtagag caggcctgcc gccgcataaa tcaggcccat gggtgttacc gtgaacatct
     7021 ggatgccgtc ataacccagt ttctgcacct ggccgagcac aactacgttg gtggatgttc
     7081 cgaccacgga gcaggtgcct cccagaatgg tggcgtagga gagggggatg agcagcttgg
     7141 aggccgcgat gttatggtcc ctgcagaagg ccagcacgat aggcatcagg atgacgacca
     7201 ccggggtgtt gttgacgaag ggggaaacca tgaacgctcc cagagtgatg accagcaggg
     7261 cggtcagttc ccttcccttg gctaccttgt tgaacagttt ggaaaggtct cctattaatc
     7321 ctgttctttc cagggaagcg ctcagaatga acatgcacac tactgtcagc ggtccgctgt
     7381 tggcaaagct ggacagcaca tcacgcgtgc tcagaattcc cgtgagcatg aggacggcgg
     7441 tgcctgtcag ggctgtgatt tccacgggca tccattcctt gatgaagctg atgaataaca
     7501 ggaccagcaa aatgcctaca atccattgct gggcggccgc tgattccagc cagcctatga
     7561 cggaggcgga gttgatgttg agaagaggaa gcatggcttg aaagttgaag gtgtggggaa
     7621 agggtccaac tctcctactt tcccggcttc tatctgtcaa gccgcaataa ttttccggaa
     7681 agggtttgaa aaggttcctg cgctttttta ttgttggtgc gccgcatgga ttcccccata
     7741 ataacaaggt tcgctcccag ccctacaggc aatctccatt tggggcacgc cctggctgcg
     7801 tgggaagccc gttccctggc gaaccgtttt tccgggaagt gcgtgctgag gatagaagat
     7861 atcgatcaga cgcggtgccg tccggagttt gttcagggca tattgaaaga tctggagtgg
     7921 ctgggcatcc gctttgacgg tcccatgatg gttcagtctt cacgcttcag cgcttatgaa
     7981 cacgctcttc agacgctgaa ggaccggggc gtgttgtacc cctgtttctg tacgcgccgg
     8041 gagattgcgg aggaggtggc cgccatgggt ggggcgccgc aggggggctg cgtggacgta
     8101 tatccgggaa cttgccggct gcttgacaag gggcgcagga aagagcttct caaatccggc
     8161 aaaccttttt cctggcggct ggattgccgt gctgcggcgc ggattaccgg tcccctgcta
     8221 tggagggaca tgcggttcgg agatcaggtc tgccgtccgg aagaactggg ggatgtgatt
     8281 ctgggccgca aggactgcgc cgccagctac catattgccg tggttgtgga cgacgccgcc
     8341 cagggagtca cccatgtaag ccgtggtgag gatttattcc ctgtgacggg catccaccgg
     8401 acgctccagg ccctgttggg gcttcctgtt ccgcagtggt atcatcaccg gctggtgaag
     8461 gatgcttccg gcaagcggct cgccaagagg gacaggagcc tgagccttca ggaaatgcgt
     8521 gccgccggga tgcggccgga agatgtgttc cggctgatgc gggagagctg agggattttc
     8581 attcccttat aggcgggggt gcgttgttcc gccgggaaag gcagggttca ggctgtggct
     8641 tgcgtttttc cagcagccgg tcgggtggtt ttctttccga tgcagagcag gcatgcataa
     8701 gccgccgcca gggtcagcag gagaagggag ctcaggatgg ccagcagatt cagatgcccc
     8761 agggggatga gccatgcatg cggcatggct gccgttcctg tcagtatctg gggaagccag
     8821 atgaaggccg cctgggaaaa caggctgacg gctgtgaagg ccagccccgc ggccagggtg
     8881 agccataccg ccggaagtac cggcaggaat gtttcatgga tgtttttttt catggcccgt
     8941 gccgttcctg cgaatatgga aacgagaaac aggggggcca gcaatgcgga ggcagggagg
     9001 gtgtcccaaa ggagccatgt gcagaggggg gagatggccg tcaggaggcc cgcgctctta
     9061 atcaggaggc cccggggcgg cggggctggt gccgcatcgt ggttcatatc cgttaatcgg
     9121 gccagacatt gccgggagtg cttccctggg taatgacgac ttcctggcct attttcaggg
     9181 tgaagttggg cgttacttcc tgctgtactt ccactagttc gttcgtatcc ttgagcttga
     9241 cgcgcacgcg gatcattttg acggcttcca ccctgccgtc cacgatggag cctgtggcgg
     9301 aagccagttt tgaggtcccc atgccgatga taccgggagt catggcctcc gccgccgttc
     9361 cggccatgtt ggcaaaggtg ccgtcgtccg tggaggtaag gacgttgatt tccttgatgc
     9421 ggaccacgtt gcccagcttg atggtcgtca ctttgccggt ggcgatttcc agattgcttt
     9481 tttgggtgca ggagaaaagg acagggagaa taagaaaagc gggaattaat ggaagcggat
     9541 tcatgatgaa cgtagttaac tacgctggca gggttaagcg tttttcatcg tgtgggaaga
     9601 attgtgtagg tagccagata taggagcaaa tggagggagg agagtgccag taccctgttc
     9661 atgcggctgt ccgcaggcgt tttgcggatt aggagcatca gatacccgcc gaagaggatg
     9721 gagggtatcc acatccacca cgtgtgggac aggttcaggt gaagggcccg gctggatgta
     9781 ggcagcgtga tgtaggcagc caggatgaaa gactgggcca tggcgcgcgc ctttccttct
     9841 cccagccgca cggccagcgt gcgcttgccc gtagtgaggt cttcctttct gtcgcggata
     9901 ttgttgacgg atatcatgac ggcgcacagg aggccgcatt gaatgcctgt tacgatgccg
     9961 gcattgtaca ccgcaagcga ttccaccaac gtgccgggca cgacgggtgc cgtgcctatc
    10021 tgtaccagaa tggttcccag tacggcaacc agtccgaaga agaggatgac gaatatttcc
    10081 cccaggccct tgtaggccag cggccacggc cctcccgtat atccataggt gaagtagagg
    10141 gacgggatgc cgatggcgag gatgggccag ccgcgcagtt cgataagggg aaaggccagc
    10201 aggcacgctc ccagcaggaa gaagaggcca atgagcatga ctgttccgtg ggagagggct
    10261 ccgctggccg tcatgcgcac gggtccggtc cgctgttctg tatcggcgtg cttggcatgg
    10321 tcaatggcgt cattgaaaaa attgcaggca atctggaggc acaggcagga agccagcgtc
    10381 aggagggcca gggacgcatt ccagctgccg gtcagtttct gcaccaccat gcagcctgcc
    10441 catactggaa tcagggaggc ggtgagcgtc ttggggcgtg ccgccagaaa ggcggaagta
    10501 atgatattca tgtgagggaa ggctccgtaa tcagcggaga atggggccga ggcggcggag
    10561 ggtttcctta atgagtgaga cgtgccattg cccgggggcc gtttcctctt ctcccaggta
    10621 gccgtatacc aggcaggagc ccagttgaga gtaaagcagg cgggagacgg ggcccatggg
    10681 ccccattccc atgacggcga tcggtccctt gggcttggtg aaaagctgta ctcctgtctg
    10741 gatgtccgag gccaggcagg gcgtgaatgc aaatttgacg atatccgcct tgtaggagcg
    10801 cgcctttttt tcctgttcgc gcaggtaatc cacacccggc gtgatttgga aatcatgggt
    10861 ggagccgatg atgatgacgt cccgatcccg cgcttccata atcagttccc tggcgtggcg
    10921 catggccttg atttcaatgt ccatggcggc ggcatatggc atataggccc ggagcagggc
    10981 aaagcgctct tcttccggga tgcggcgcag tcctccttcc tcatggcagc gcacggtgac
    11041 cagcaggggc atctccggcc ggaactccat aacctgttca gtggtgagtt ccgggggaag
    11101 ggcatccacg cgcagttcca ctagatcgca ctggtctgtt aaatcttttc ctttcagcgt
    11161 tttccatagc tcccacgagg taacggacgc aacgattttc ggcggatagg cctgatgttc
    11221 acgtacaatc tgctgcatac atcctactat cccagcaaag gaaggaaggt caatcttcta
    11281 ttgatgagtt ccacttcatt ttgggaccga gtatgtcagt tcgcctgcct tttccctttt
    11341 ggaactgtgt ttttcttcct gtgccaaccc tgttggccgg tttcccggca gacggattga
    11401 cggatgttgg cgccggctgc cgttcaggat gtcggcaggg ctgtttcgta tggcccgttt
    11461 ccttgttgtg gcggagggcg ttattttcct gcctctgcac gttaaaaaca tgttgagaat
    11521 ggaggtccgg cttgaaaagg gagttcggat ggagctaggt gaaagccatg aatatgcttt
    11581 ggtcaaggtt attgcggatg gggcggaatc ccttttgggc ggctttggat gccgtgctgg
    11641 tggtaaggac ggaaaaagat tcgtccggac atccggaatg gtcctggaag gcgtatgtcc
    11701 ctcatggcgt tccggttcat gaattgcctc cggtgcccgt tcctcttccg gaaggggatt
    11761 gggataagga gcggtttcgt aaggaaagac ggaaagctgc ccgggtgctg ggtcaccagg
    11821 ccgctttaac gctggcttct tccctccatt tctcccgttt catgattgtg gaggaagagt
    11881 gccgttttag gctgccggta ataggttcca cggcgcggat gcttgggcag ctgctgaaag
    11941 gctgccggga aagaactgct ctgtttttaa gtgagccgcg tttgtctccc catacggcct
    12001 gctccgtggc gaaaggggaa aaatccatcc tgttgcgcgt aacggatatg gcggcatcat
    12061 ctgcgtatgt gatggacctt tccttatgtg aacgcattct tttatctctt ccgtcagatc
    12121 agcgggtctg gccatggctg aggtattatg gttctctgga ggaatggttt gcccggtatc
    12181 tgggacgtta tggtgattta tatgctcttt cccctgcgtt cgtttcctgc ggagagggcg
    12241 ctcccattcc ctccctctgc aaatccctgg gccatttcaa taatgcacgc accaaatata
    12301 cggatctgtc ttatctggtg aatccggcgg tcaattcatg gcgctggctg aaaaacgtga
    12361 acattttttc cggcagaaaa aggcttgggt gagaaccccg gggatgcgtt ttgctccggc
    12421 ttcgccggat taccgtttgc gggggatgaa cgcctgcatg gctggggctg ccgtcttacg
    12481 gcagagcttg aaataagatg ttccgtgcgc ccatcatctc aaaagcaaaa ccccggaccg
    12541 caggcggtcc ggggcggctc cctttttccg gagggaggat gccgtgatat tagaggggtt
    12601 agctggctcc ttcgtcacac aggatcttga caaagccttc ctcctggagg cgggtggcgc
    12661 cgcaggcgaa cttggcccgg atttgcaggg cttcctccat atcgtcgcgc acggaaagct
    12721 tcactttgaa atcattccat atgccaaact gggccttgct ctttacccag gccaggcagg
    12781 aacggactcc ctcttctgtt ttgggaagcc tctgggtgcg gatgaatttg aatcccatga
    12841 aggtgtctat tttcccttcc accagggctt ttacattgtt gaagtcataa ctgctgacct
    12901 ccgtttcccg cagcaggttc atgatttggg aactggttac cgcaatgacg agctggtctc
    12961 cgaactgcgg ggcatcctgg ttccaggctt cgttttcctc aaaaagctgg agggctgcgc
    13021 gcagcttggc cacggtaagg ttgctggcgg atttggtgcc gctgtccacg tgatccacgg
    13081 ctatttgctg gctttccttg aacggaacgg cggtcatgcc gtcttctccc acgtagttcg
    13141 tatccagaaa gccggagatc atcacgtcgt ccatgcggcg gcctgctgcg gcctggagac
    13201 cttcaatcgt cttgctgacg ggaaggtcca tgtctcccag gcgggtggcg tcaaattcat
    13261 cgtagccaat ggctttggtg aacgtgcgcg gccgcatgga ccggcgtgtg gtgggagcct
    13321 cgttcaaaac ggtctgcccc acccgcgtgg ttttttccgt gaagtccaga atgccgaatt
    13381 gatccagaaa tactactttg ccgctcaagt cgcgcatgac gctgacatac ttgtccagcc
    13441 gggaggcgtg ctgctggagc agggtgcccc attttctggt gtattttacc tgataattat
    13501 cgcagatagt tactgccatg attgcaaata taatgatagt ttgaatgcag gaacggcaat
    13561 ttggattatc gggtccggaa taaccggagc ggtcctgccg caagcgggat gcggccatgt
    13621 cccctgcggg attgcgggcc ggtcccatcc agcgctaacg tatatggact acaaggcaga
    13681 aaagcgcacc acggcagaat cgtgatgcgc tgtataagga atgaaaggct tcgccttccc
    13741 gctgttaaag aataatcgcg tgcgtccgcg tagcgtgatc cgggccgaaa agagccagca
    13801 tgcggcccgt ttgccctttt tccatgcggt aggagtaaaa acgcttggta tccgccgccg
    13861 tgtccagccc ggaatcatga atctgttctt ccggtacccc tgcttcaatg agctgtgcct
    13921 taatggctgt aggaatgtct tcctcataat gaggcgggcg gatacagggg ggaatgacgg
    13981 caatcaggtt ggacggttcc accccaaggc attttttcat ggacttgatg gtgttggaaa
    14041 cgatgttgcc ggcgattccc gccttgcctg aatgaatcag ggaacggtgg cagttttcct
    14101 tgtcgtacaa ccacacggcg gcgcagtccg ccacgtaaat ggcaagcacg cagtcggaaa
    14161 ctccggaaca aatcaggccg tccgcgcctt ccacaggata attggagccg atgtccccca
    14221 ccagggcaac tttcgtgccg tgcacctgtt cggcgcggcg caggtctttc caccgccagc
    14281 ccagggcttc cacttccgcc ttatggtacg gttccagccg ttccagcacg gtttcgcgat
    14341 ctccggcgac gtctactccc ggaattctct caatgaattg gacgatttct ccgggatagg
    14401 actggatagg cttcaagaat gctggcgttt ccataattga tggatcaata aatgtcgttg
    14461 gggacatctt gtcgttttgt attacctagt cgagcaaaat ttggcgtaaa atgtcacgga
    14521 cgccgcccgg tgactgagaa aaggtttttt tccgcctttc cggagtgata tgagcaattc
    14581 atgctgcgca ggtattcttt actttccact ttttttgcgg gaatgaccgc cgtgtttttt
    14641 tcctttgccg cctccttggc ccaggaggct tctccggatg ccgggcagct gctgaaaagg
    14701 gtgcgccagg gggcaactct tcaggaaaac aaggacatta aggggcaaat acgcaaacgc
    14761 agcgtcaaaa ttcccttttc catgagtctg cgcggcaatc tgatagcctt ccagtaccag
    14821 ctcaataatg tctggaacag gttcgatctg aaattcaagg accgcggcca ggagatcctt
    14881 tcctggaagg acgggaaagc cggtgttttg ccggttgccc agtatgcggt gcccattgcg
    14941 gggacggacg taacgtacga agatctttcc atgcgctacc tgtactggcc ccaggcaaaa
    15001 attgtccggg atgatgcggc gagcaccgtc aaggggcggg attgctggat tgtccagata
    15061 ccgaatccta attccggggt ggggcaatat gcctgggtgc gcgtctggat tgataaggag
    15121 aacggcgcca tgtggcaggt ggatggcatt gaccgccgcg gggagctggc taaaaggttc
    15181 atgattgatt ccgtgatgaa attgaaggac ggatcctggt ttttcaaacg gatgaaggtg
    15241 gaggtccggg atccttctaa tccgcgcaga acggtctccg tcagctacat agatatggac
    15301 agcccggaat agccttccgg ttcccgttcc attccccgcg gagctttcgg attattcttt
    15361 ccggggcatg ggggcggatt ccttttcatg ccgcacggat ggatgattta ggacgtgaca
    15421 agaaggcggg atgggcgtaa atcctccaaa aacctgttat gaagaaacat ttattccttg
    15481 catgtgccct ggcgttcggc atgtccgcct gttcccagca ccaggagcag ccggcggagt
    15541 ccggttctcc ggtgaaaacg accatttctc ccgtgactgc tcctgggaaa aagccggacc
    15601 ggccggccgt gaaattgcct cccaagtccg ttccggtgcc ttccgtaagc ccgtcctata
    15661 ataatactcc tctttccccg cgcattccgg gggtaacggt caaccgtgtg gctgtgccgg
    15721 acaaggtggt ggccctcacc tttgatgacg gtccccatgg aacgctgacc cccagggtgc
    15781 tggacatcct gcgcgacaat aacgtcaagg gtactttctt catgcagggc tgcaatgtga
    15841 aggctcatcc ccaggtagtg cgccgcatgg tcaatgaggg gcatgaagta ggcaaccata
    15901 catggaatca cgtttatctg agcaaagtgc cccgcgaaaa agcggaatcc cagctccaga
    15961 gcactaatga agccatccgc aacgcctgcg gcgttgtccc cgtggtcatg cgcccccccg
    16021 gcggctatac gaatgcaggc gtggcgtcct gggcccgcca gcgcttcggc ttcaccacgg
    16081 tcatgtggga tgtggacacg aatgactggc gcaagcccgg ctcctccgtg gtggccgccc
    16141 gcgccgtgaa cggcgccaaa cccggctcca tcattctggt gcatgacatt cacgcttcca
    16201 cggtggcggc ggtggacgcc attgtgaagg gattgaaaaa ccgcggctat gaactggtga
    16261 ccgtttccga acttatcagg agaggacgtg ccgccgccgg ccatgctcct tccatgcccg
    16321 cggctcctgc tgtcccggat tcttctcctc ttgccgttcc ctcgattgtg gctcccgcgg
    16381 agcctgttcc cgctccggcg gagacggtgg ccggcatgta atccccggtg tttttccttt
    16441 tcctttcaac ccggtttccc tgcggaaacc gggttattca ttgcctttcc ggagcatttt
    16501 tcccaccgcc ccggaaagga ggctttcccc ttaacgggca cacgcacaga aggcttgacc
    16561 tgctggtgaa tttctccaca atcctttgcc gtattattca cacccaatcc ggattgaaat
    16621 gtctatgaat ctcctggcat ctgccgatac tctacccgca cctggcggaa tgtccgctcc
    16681 tctcgccttc atctacgatc tcttcaataa ccctcttttc atcttcattg gcggtctggt
    16741 gttgctgggt gtgtttttct ggtacctggg ttccgaccag gacaaggtca agcgcaatgc
    16801 cggaacgatt ttcattatcg gcattgcgtc tttttccctg ttctccctct tccagcaggg
    16861 gttgaaatac ggcattgaca ttgccggggg tgtctccttc acgttgagcg tggaaccgaa
    16921 tatcggagac gacggcaagc ccattcccct gacggaccag gccatggaac aggcatgcgt
    16981 gatcctgacg gaccgcctta acagtaccgg ggccaatgac gtcattatcc gcccgaagaa
    17041 aaaagacaac ctgaatctta tagaagttca aatccccgct gcggatcctg ccaagcgcga
    17101 ggaaaccaag gccatcctga ccaaagtagc caaacttcag attctccctg tccacccgcg
    17161 taacaacgaa ctggtggcgg aggggcgcat ccgcgttccc ggctacagga tgtatgaata
    17221 taccttcaaa aacggcaagg gtgaggacgt cacggaaaaa atattcctgg aaaagcgtga
    17281 atcgctgaca ggcagggata tcgtgcgcgc cggagtggat ctggcccgtc ccggccacgt
    17341 caacgtcacc ctgagcaacg aaggggcgga caagatgtat aacctgactt cccgcatgca
    17401 gctgggccac gaccgcatgg ccattgtgct ggatgatgtg gtcaagagcg ctccaacggt
    17461 ccaggccatt ctttcgaaaa gttttgaaat cagcgggctg gatgctcccg gagaggctga
    17521 agccctgacg aaggtgcttt ccaaccccct taccaacaag ctcaattttg agtccgccag
    17581 tgaaatttcc gcctctttgg ggcatacggc cctccttcag ggggaatacg ctggcattac
    17641 gggattgatc ctctgcttca tcatgatgat catctactac cgctttgccg gtttggtggc
    17701 tatcatgggt ctgacgatca atgccctgct tcttctggga gccatgtcta tcttcgggtt
    17761 tgagctgacg ctgccgggta ttgccggtat tgtgctgact ctgggcgtgg cggtggactc
    17821 gaacgtgttg atttatgaac gcctgcggga agaaaaggaa atggggcgcc ccttccgcgt
    17881 ggctatccgc aactcctttg acaaggcgtt ctccgccatc tttgactcca atatcacctc
    17941 gctcattacc gccgtcatcc tgtactggat ggcaaccggc accatcaagg ggtttgccgt
    18001 cacgttgaca gtaggcgtca tcacttccat gattggggcg attctcgtta cccgcgtcct
    18061 gttctactgg gcggataccg tcggcatgat gaaggatctt aaattcctca acctcttcaa
    18121 gaaaaagacg aacatcgact tcatgagcca gaaaatctgg tcctgctctc tttccgccat
    18181 tctgctgatg atatgcattg tatttggcgc cgtaaagggc aaggattgcc tgggcatgga
    18241 ctttacgggc ggttcttcca tttcctatct ggtgaacaag ggtgacatct ccttccggga
    18301 agtggaaggc gtggtcaaca agctgtccct gacccagaag gctacggtgc aggaggtggc
    18361 ggagtccacg gacagcagca aagtgaatat tctgattaat ttctccgacc atgcccagga
    18421 caaggaggct atcaccacgg cattggacaa ggcgtttccg gttctggaca acgctccgtt
    18481 cagtgaagaa accatcgggc agtccatggg gtacgatacg ctcattactt ccgcatgggc
    18541 ccttttcttc ggcattctgg gaatcatggt ttacctgacg gtgcggtttg aatggacctt
    18601 cgccattggt gcggtcatcg ccctgacgca tgacgtcctg ctggtgcttg gcctggtgat
    18661 tgtcagcgga acggagctga atgttattca tattggcgcg ttgttgacgg tggcggggta
    18721 ctccatcaat gacaaaatta tcgtatttga ccgcatccgc gaattcctcc gcttctccga
    18781 cccgaatgaa agcgcttcac aaatcatgaa cgaagccatt aaccagacgc tttcccgtac
    18841 tattttgacc tctctttcca cgctggccgt gctggtgtgc ctgtacttct ttggaggtcc
    18901 ttccatggaa gacttcgcct ggacgatttc cgcaggcatt ttgattggta cgtactcctc
    18961 catcttcatc gcttctccgg cggctctttt cttctcccgg aagcatgggt tgcatgaaga
    19021 agtcagacag gccatgaagg ccggagcctg aggtttggcg ttgagccgtt aaatctctga
    19081 ccggggaagg ctgaatgcct tccccggtct gtttcttgga aagagaggga aaggctctgt
    19141 atggaataac ctccgtgttc ttttcccgtc cgcctctttc gcttttcctg tttccaccat
    19201 atgcggctac ggcttccgca tggctcttct gtctcgcatt cctccctctt ttatttcagg
    19261 cataagaaaa ccccgcctgc cgttaggcag acggggccgt atctctggtt tcctgctatg
    19321 tatttctgcg ggtgagatta ttcatcatcc ccttcctcct gtgccggggc gggaggggga
    19381 ggcatgtcgt tgttgccgcg ggctgccttt ctggcttccc tgcgcgcttc aagagccttg
    19441 gaagcgtcag cgttgagctg ggccatttca gcctcattca attttccgtc cttgtcggcg
    19501 tcatactttt cttcaatcag gatccatccg gcagccatga tcggggcgcg gttggggtca
    19561 agctggggac ggggaccgtt agggcggttg ccgtgggggc cccggttacc gtcttcccgg
    19621 cgtccttcac cgcctctgcg gccttccggt cctctgggag cgcgggggcc ttcagctctg
    19681 gggcctcccg gacgcctcgg accgtccatg ttgggcattc tcggtccttt ggggccaccg
    19741 ggaacggcat ggtgcgggcc gggctggccg tgatgagggg gaatggactg caattcttcc
    19801 ggggagagct tcccgtcgcc gtctttgtca aattttttga tcatggcggc ggcgtgggct
    19861 tcctggcggg ctttcatttc ttccatgcgc ttggcagctt ccggatggtt ttttgcccaa
    19921 tcttcccgca tggctttctt ttcctcgtcg gaaagcttgc catcgccatc cttatcgtac
    19981 ttcttaagaa tttcggcttt cctggcttcc tggcgggctt tcatttcttc catgcgtttg
    20041 gcagcttccg gatggtcctt aagccattct tcctgcatag cctttctttc ctccttggaa
    20101 agcttgccgt ccccatcctt gtcaaatttc ttcaggatgg cggctttctt ggcttcctgg
    20161 gccttcttga cgtcggcttg cagaacggct atttcctcgt cggaaagctt tccgtccttg
    20221 tcggtatcgt atttggccaa taccagttct tctccgacaa tcatgatacc gggatcgatt
    20281 ctcttcatct gaactcgttt tggcccctgg cggtctctgc ctccggcatg gtggcgatga
    20341 cctccccttc tttcaggggg gggctgcatt tcaggagcat cggaaggagc ctccggagcg
    20401 ggaggcggcg gagcgtcctg agcgaacgcg gaacatgcgg cgaaagccgc ggcgataatc
    20461 atttgcttgg tcatatcttt gaatggtgag gtttgatgcg tttcggaaag gagcgtgcgc
    20521 tgcggtgttt caaacaggcg gaaacgtctc cctttgctaa aaggaacccc tggaattcat
    20581 tccggttgca aaataaaaag gcaannnnnn nnnncggaga gggggtgcgc cgctgttttt
    20641 atgacacctg ttttcttccg gggtaaagaa gggtgaaccc attgaatatg aaggaaatca
    20701 gtgaggggat acgcaatcgg gtgcgagtgc gtgccgcccg tgtcatagcg gaaaaaatta
    20761 ttcgccggaa ataaagcgga tgtaggcctg ccaccactgg ctgccgccca gcagccagat
    20821 cagagcggcg ctgagaagag cggggccaaa ggggaggttt ctgccgaacc ccatgcgtga
    20881 ggcggcagcc tgtacaatcc ccagcaggga gccgaggaaa aggctgaaca gggcgccctg
    20941 ccagccgcac gcgcatccgg ccatcatcag aatccaggcg tcgcctgatc ccatggcttc
    21001 cctgctggca tggatatccg tcagatttcc ctccacgctt tccagtcttt ccagaagaaa
    21061 aacgtccccg ttttccatct ctatcctgtc gtaccgcagc gtgacggagc aggcagggtg
    21121 cgttttgccg tcaatggtga cggaacctcc ggaaaggacg gccttgtccg tagaccggtg
    21181 gaaaagcatg gaccagtcgt gaggctggcc gttgacgatg agctgaagtt cctcccgctc
    21241 ggattccggt tccctcaaac tccacggggc gggaacgtca aaatgctgtt tccaggtgcc
    21301 gaacagaagc ttccccaatt caatcaccag cctgatgacc gcataccctg ccgctcctcc
    21361 cagcacggat gccgtgaagg cgtccgtcca ggtcatgaca tggctgtccg gcaggaggaa
    21421 agggtaaagg gcgcaggccc ccgttccggc caaggcgccg ataaatgcct gagagcggaa
    21481 gacaatcaga tgttccgcgt caatgaacat gatgacaatg gccatggcag tccacaggca
    21541 gagcaggacg gctgctcctg tggaagcgtc cgcgtatttc cagccaatgg ccgcgaagag
    21601 cagggccgtg agcagctcca caaagcaata tcggaagctg atgcgcgttc cgcagcaggc
    21661 ggatttaccc cggagagcca gccagctcag aaccggaata ttcaggaacc agggaatggg
    21721 ttttccgcat tccgggcaga aagaacgcgc cggatcgttg acggagacgc cccgcggcat
    21781 acggtagata accacgttca ggaaggaacc tatgcaggcc cccatcaggc cgaaaaccac
    21841 gggaatcacc catgcgggaa aggcgtcagg catgggtggg cggggaatag gttcggctgg
    21901 gcgtctggtt gatgcggtca atcagcagcc tcagcctgcc gtagttgccg cgtttgatgg
    21961 ggaagacgag ccggggcagg cggtccaggt gtggttcgtc cgcttcatcc ggcagggggc
    22021 cgctctggat cattttcccc ccaggcagaa tcaggtctga aaagtcccgt tccagatgtt
    22081 ccacccactg ggcgggaagc tgcgcattca gccggatgac gatggaatct ccgacaaaac
    22141 ggtaggagtg gaaaatccgg taaaaacggt cgatgtgttc catggcttcc gccggatttt
    22201 tagtgacatg gatgagatga agatcgtctg cggaaatcag tccggaatcc accagttcca
    22261 cgcgaatgaa ggccagccag ttcagccaga aggttttgcc gggggaatcc agaaggacga
    22321 tcgggtaaat ggtcgctttt cccgtctgga tcagagtgag cgtttcaaac acttcatcca
    22381 tggttccgaa gcctccgggg aatgccacca tggcgtcgct ttccgccacg aagttcagtt
    22441 ttctgacgaa aaagtaataa aaattgatga gtttgtcgct gtgggccacc acatggttgg
    22501 aggtttgttc atacggcagg gtgatgttca ggccgaagga gcgttgttct cccgcccctt
    22561 cattggctgc ctgcatgatg cccggtccgc ctccggtaat gaccatgtag ccgtgttcgc
    22621 tggcttccct ggcgaattcc ctcgccgttt catacgccgg ttcgtcattc ctgatgcggg
    22681 cggagccgaa gacggaaatc ttgcggatgt ggcggtacgg ggcgaaaacg cagttggcgc
    22741 ggtacatctc ccgcccggag cggatcagga cggtaaaatc atggtccgag gcggcggact
    22801 gggccatgtt gattcccgtt ttgatatact cttggaggta gcggggatcg tgcctggtgg
    22861 aggctatttc ggaaagcttt ttaacggcgc cttccagagc ctggtagctg gaatccatgg
    22921 acatggtgga gggacgtttc ttgtcttcac ctgtaaaatc cctgttgtcc aaaaaatcag
    22981 gatgcttcat gagttggtat gataggtttg ccctactatg ccagatgacg cacattaagt
    23041 cacagaaaat ttaccggcgg gcatgaaaag agttgattgt tgaaaacaga tcgttacatt
    23101 gctccccgca cgggcgttcc cacaacatgc cgttggtcct tcaggcgcat tggccgccat
    23161 gtccgtgttg aggggaaacc cggtacatta catacgatta tgtcggaaaa cgaaaccact
    23221 actgcagaag aaaccgccgt taccacgctg gcccgttttg aggttcccag ccgtctggaa
    23281 aaaattgaag atccgaatga tgcgaatcat ttgacctttg tggcggaacc gtttgaaaac
    23341 ggttacggcc atacgcttgg caactccctg cgccgcgttc ttctgggttc tcttgaaggc
    23401 gccgccatta cctccgtccg catcgccggt gcgcagcatg aattttcctc cctgcccggt
    23461 gtggtggaag atgtgacgga aatcgtgctg aacctgaaaa aggtcaaatt caagcataac
    23521 ggcaaggaac cgcgcctgct ctctctgcgc gtgcataagc agggcgttgt gactgctgcc
    23581 gatattgccg acgacaccgt ttaccaggtg gtcaatcccg atcaaatcat ttgcactctg
    23641 gatcaggaca ccatgttcga atgcgaattc caggttcgcg tgggccgcgg ttttgccacc
    23701 ggcgatgaaa acaaggtgcc ggatatgccc atcggcgtga ttccgatcga ctccattttc
    23761 tctccggtta cccgcgtgaa gtattctgtg cagaataccc gtgtggggca gatgactgac
    23821 tacgacaagc tgatccttga agtctggacg gacggccgca tcgcccccgc agacgccatg
    23881 ctccaggctt ccgccattct gcgccatcac ctggacgtat tcgtcaacta tgacgacaag
    23941 caggttaact ttgaggaagc tccttccgcc gtccaggacg aggaaacggc ccgcctgcgc
    24001 aagttgctca acatgagcgt caacgaaatc gagctttccg tgcgtgccgc caactgcctc
    24061 aacaatgcca atatcacgac cgttggccag cttgccatga agagcgaaag cgaaatgctc
    24121 aagtaccgca atttcggcaa aaaatccctc aacgaaatca aggacaaatt gctggaactg
    24181 gggctctccc tgggcgttca ggtggatccc tccctgctta ccggtcccgt gccgcaggcc
    24241 aagccgcgcc tgggtctgga agaggaaagc cccgtgggcc tggcagacct gattgccgcc
    24301 aacatcgaag acgacgacga ctaaatctca caaaaccaac acacatagaa aaatatcatc
    24361 atgagacacg gtgtaaaaac ctccaagctt cagcgcaacg cttctcaccg cagggctctg
    24421 ctcgccaacc aggcatgcag cctcatcctc aatggacgca tcaccaccac cctggccaag
    24481 gccaaggccc tgcgccccta tgtggaaaag ctgattacgc ttgccaagcg cggtgacgtg
    24541 cattcccgcc gcctggctac cgccaccatt cataatacga ccgccgttaa gcggctgttt
    24601 gatgaaatcg ctcctctttg cgcggaacgc aagggcggct atacccgcat cgtcaagctg
    24661 ggccagcgtc tgactgattc cgcccttgtg gccatgatcg aaattatcga tctgccccgt
    24721 gaggctgccg aaaaggaaga agctcccgcc accacggaag ccaccgccta aaacgcacct
    24781 ctggaacatc ctgttaatac cccgctccgg ccttttgtga ggccggagcg gattttttat
    24841 tggcaataag gaagttatgg tgatgatatc gctcttgctt ctttcctgcc ggaacgttat
    24901 aatccagctt taactatgga cgcagtatta ttgttttccg gacaaggtgc ccaaaaagta
    24961 ggcatgggca aagatttgta tgaccagtat cccgtcgcca aagccctgat tgaacaggcg
    25021 gacaaggcgc ttggagaatc cctttctgcc gtcatgtttg aaggccccgg cgaagaactg
    25081 acgcgtacct gcaactgtca gcctgccctc tacgtacatg gccttgcctg cctggccgtc
    25141 ttgaaggagc tcgttccctc cctgaatcct gtggctgccg ccggcctttc cctgggggaa
    25201 ttcacggctc atgccgctgc cgggacctat tcttttgaag aaggcctccg ccttgtccag
    25261 aagcgcggcg cgtttatgga ggaagcatgc aacgccacga aaggcactat ggccgccatg
    25321 atcggcggca cggatgaaaa cgtcattaag ctggcgcagg aatgtgacgt ggatgttgcc
    25381 aatttcaatt gccccggcca gacagtcgtt tccggtacgg aggaaggtgt ggccaaggct
    25441 gtagcagggg cgaaggccgc cggctgcaag attgccaaga agctcaatgt ggcgggcgcc
    25501 taccattccc gtctgatgca cagcgctatg gtaaagctgg cggaagaact caagaatatt
    25561 tccttcggta cgccgtccat gcctgtttat tgcaattatg aagcccgggt agtggaaggt
    25621 ccggacgaca tccgcagcat gctggaacac caggtatgcg gatccgtgcg ctggacggct
    25681 tccatgcaga agctggtgga ccagggccac cgccttttca ttgaactggg gccgggcaag
    25741 acgctggccg gcatgatggc tcgcatctgc aaggaagcta cggtcatctc cattgaagac
    25801 gtgcccagcc tggaagccgc cgttgcggag ctgaacaaat aatttccctg aataaaaggg
    25861 ggtaagccgc cggcccgttg gggagccggc ggttttttgt tggcgtgttc atggcggggt
    25921 tccgtcaaat aggcaatatc ccatggaaca ggggaaatgc ctgcctccgt gccagcggcc
    25981 ctgctcctgc gcgggcattt tcctcatgcc gccggtggga agatgcatga acatcagcat
    26041 cgttctcatg aaaaaagcga tggagggcac gaacagcggt ttccatataa ggaaaagtgc
    26101 gggcatgatg gaatgcgccg ctcctcaggt cagaagaatc agggcaatga ggtaaaacag
    26161 gaggccgctt ttaggaggcc ggcaggagag catgaaagcc gaggctctga gccgcgccag
    26221 gcgttcctgg cggcgcagcc ccctgcatca ggcatgaaag gaattccgtc aggaaacagg
    26281 cgttatggaa tagaaattcc tgatttctaa agaagttccg tcagagagcg gatgatactg
    26341 gtcgccaggg aaaaatccgc ctgctccgtt accctgtttg gaaccgagat gactggcatt
    26401 cctgcgcggt gtgcggctgt agtgccgttc tgggagtctt ccagcaccag gcattcagac
    26461 ggagatttac ccaggttttc cgcggctttc aggaacaggg ccggatccgg tttgacgggg
    26521 aggccgtcgt cgcggcagac gacggtttga aaatagggca tgatgcccaa tctgttcagc
    26581 cagccgtcca cccagcggtg ggaagaactg gaggccactc ccataggggt tccggcttcc
    26641 cccaggttcc ggatgagttc tccggcgccg ggcaggaggc cggcatgttc cagatcccgg
    26701 acgatttcct cctggcggcg gctgttgacg gtttcccagt cgaaagtgcg ccccgtcaat
    26761 ttctccagat gttctccggg attccagtgg gtgtagccgc tgcccaggca ttggttgaac
    26821 aaatccaggg gaagagggtg gccgcaggac gcgaaaaccc gttcccagga ggaataaatg
    26881 gcatattccg tatccaccag caatccgtca aaatcaaaaa tgacggcgtg tgcccgccgg
    26941 agcagttcac gggctttcag aacggcttcc cgggaatcgg caaagtgatc aggaatggtc
    27001 atggacggga gtctttttca agcctctcaa taattccgag aagcttctgt gagccagctt
    27061 ggtttgaaaa aggcggatgg cgatgatggt gtagataatg ttgggcatcc atgctcccag
    27121 ccaggtgggg aggtagcctg cggaggccag ggtggggaaa aactcataca ggaacagcat
    27181 ggctgccgcc aggaacaacg caatgccgat gccggacatg gcggagcgcc tctggaacgt
    27241 gatggcggag gggatggcaa taaatgtcag gataatgcag gaaaatatgc gggcgatcct
    27301 gacgtgccat tccgtctgca ggcttctgag gtacctggcg tttgtcgtgc cggacttgat
    27361 gagttcctgg agagccgggg ttccctgtgt atccacgcgg acgttgggtg aaatcagttg
    27421 ccagggtgtt tcgggataat gctctttcag cgtttggtat tcctgtccgg caaaaacggg
    27481 aacatcatgg agctggcggg gttcctcctg ggaataattt cttttgatgg cctgccggaa
    27541 ggtccatgtc ctggaggccg gatcccaggt ggcttcatcg gcaaacagct cgtatttcat
    27601 ctttccgggg gcgctgaact gttccacccg cacctgccgg aaaggattgc cgggagaatc
    27661 aattccggga ggattgccca gataccagat gcgggattcc gcatcatttt tgtagatggt
    27721 gctctgggaa gcatggttat ttttgttctg gctcagggag gagaacatca gtttcctgta
    27781 cagggcggag ttgggtgccc agtggaaccc gaagatgccg aagtagatgg aggcaaagac
    27841 ggcaccgatg atgatggggg tgttgattcg gagcagggac cgtccggact gcagcatgcc
    27901 ggtgatttct gaggatgagg aaagtttggt aagcgcccag agtgttccca ggagaagggt
    27961 ataaggcaga atcaggttca ggatcatggg cagctggctc aggtaaaagc ggaacgtgcc
    28021 catgaggggt gcgtccaggt tcatcaggtc tcccacattg tccgcaaaat ctccgataat
    28081 gtaaatcagc gtcaggatgg ccgtacacat gatgaaggcc gtgaaccagg tgcgcgccac
    28141 atacttgtcc aggatgctcg aacggttgta caggaacatt cccagcgcgg ggagaaagca
    28201 gcccagaagg atgagccagg gccgcaggag ctgggaaagg ggatattcgg acggcgtacc
    28261 gggaatttcc cattgcatct gggaccattc ccggggcatc agaaaccagg ctgccgctcc
    28321 tcccagcgca agcaggagga gaaatgcgga ccattggcgg aaatgtcggg cctggattgc
    28381 catgaaaaaa gagggtgggg caagaatgcc agatcgggag cgggaaataa agcgttattt
    28441 ggccgccgga cagggataaa aggctgccgg aaccgcatgt atgtgcttgc ttcgtgaacc
    28501 tcagtgcggt tccgctcctt aaagggagga agtggctgcg ggagatgttc cgcctgatag
    28561 aagttggctg ttgtcaacgt aaactggatg agataaaatc ttcctgacaa ctgtttcccg
    28621 gcaacagcct ttccatgttc aaacgtcttg ttcaattgtt tcttctgttt tttctggctg
    28681 ctgcgggtgt tctggcgtgg tttgcggggt acgtctccac tcctctgctc attcccgtgg
    28741 gaggggagga acccgcctct tctcccgttg cgggttccct ggtgatggaa cccgtggaag
    28801 ccgtttcatc cgatggcacg gctattgaag gttatctggt gagcccgggg cgtccggagg
    28861 ctttgtctcc gcgccagagc cgcgtgcggg atactctgaa attgcgtggg aaaatgccac
    28921 atttggaagc aaaaccggag ctggtcgtga tagccgcttc ttggaatgag ggagtggaaa
    28981 gttccctccc tc
//
LOCUS       DAOMSU010000004        26961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_45_length_26961_cov_2.873374, whole genome shotgun sequence.
ACCESSION   DAOMSU010000004 DAOMSU010000000
VERSION     DAOMSU010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 26961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26961
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45_length_26961_cov_2.873374"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="PK077_00395"
     CDS             <1..351
                     /locus_tag="PK077_00395"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRN22791.1"
                     /translation="KGVPDSELPRTESLKETIARMMPYWEGTVLPSLRTLDNILIVAH
                     GNTLRGMIKYLKNIPDEQLLSLNLPTATPYVFEFYDSLNLGKDYFLGDPEEIRKRMAA
                     VAGQGSAKKKTPAE"
     gene            369..>1324
                     /locus_tag="PK077_00400"
     CDS             369..>1324
                     /locus_tag="PK077_00400"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HRN22792.1"
                     /translation="MTVHSCRIAFFDAKPYDRDSFNSVNEREFHYDIRYFKGHLTPDS
                     VPLTRGTDVACIFVNDTANREVIRNLKENGVKLLALRCAGFNNVDLKAAEEAELPVVR
                     VPQYSPYAVAEHAVALMLSLNRKIHRAYWRTRDGNFSLHGLMGFDMNGKTAGIIGTGK
                     IARILIRILKGFGMNILAYDLHPDQRFAEEAGITYTTLDDLYARSDIISLHCPLTPET
                     EHLINTDSIGKMKDGVMIINTGRGKLINTEMLIDGLKSKKVGAAGLDVYEEEGEYFYE
                     DKSDRIIDDDTLARLLSFNNVILTSHQGFFTKEALHNIAEVTL"
     assembly_gap    1325..1334
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1530..2678
                     /locus_tag="PK077_00405"
     CDS             1530..2678
                     /locus_tag="PK077_00405"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRN22793.1"
                     /translation="MSTPAPASVLAPKPHYAALDGLRGVAAVMVVLFHMFEGSARDFQ
                     FHMDQIINHGYLSVDFFFMLSGFVIGYAYDDRWEKMNLWNFCKRRLVRLQPMVVMGML
                     LGGILFYFQGSEIFPNLAQTPVWKMLLVMTVGFTLLPVPVSLDVRGWSEMHPLNGPAW
                     SLFFEYVANILYAMLIRKFTSRWLAVLVFLAGCALIHQCYTQGNNIGGWTLDAEQCRV
                     GLTRLMFPFFGGLLLFRLCKPGRIRHGFWWCSLLLAGTLAAPHVGGAEHFRLNGLYDA
                     FCIIVIFPVIVFLGASDIPGDISSRASRFLGNISYPLYITHYPIVYLYLAWVHDAEVP
                     LSRALPVAAGVLFLCIAVAYGCLKLYDEPVRAWLSKRVLKRHPPEPGA"
     gene            2741..>2954
                     /locus_tag="PK077_00410"
     CDS             2741..>2954
                     /locus_tag="PK077_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN22794.1"
                     /translation="MEKLPTSSKALRYLPFLVASAFFMQMLDATILNTAIPTIARSFN
                     SHPLQLHSLVTAYMLTVCVLMPASGWI"
     assembly_gap    2955..2971
                     /estimated_length=17
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2972..4150
                     /locus_tag="PK077_00415"
     CDS             <2972..4150
                     /locus_tag="PK077_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN22795.1"
                     /translation="RRTFLLAISVFSVGSLLCALSASVTMMTVCRVIQGIGGAFLMPV
                     GRLVILRSYPRSMFVNVLNIVTIPALLGPLLGPVLGGIIVQYASWHWIFLINIPVGMA
                     GLWATWKLMPDLKAVREQKFDWPGFFLFSSSAVLATMGLSSAGGITDKPRMVILTSAG
                     GILQLLYWVLAFRSKAPIFSPALFRIRNFAIGIAGNIVCRLGGSCLPYLMPLFFQVVL
                     GYSALQSGMSLIPLALSNLLAKTVAPRLLGKFGYRNIMVVNTFTIGALLASFHFVGPG
                     THELILLGMLALLGGANSIQFTCMNTLTLIDLPNADASSGNSLLSTIMQLSIAVSIAA
                     ASLLLDSFGGHSATSGKAVETAFHTTFATIGAIAAASSFIFALVDKDKGITRKRKKAA
                     "
     gene            complement(4173..4640)
                     /locus_tag="PK077_00420"
     CDS             complement(4173..4640)
                     /locus_tag="PK077_00420"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HRN22796.1"
                     /translation="MENTYRHAQLPEGRVPALRVEPMPADTNQSGEVFGGWVMSQVDL
                     AGANTAMRYALSRYIVTRAVSSLTFEAPVLVGDVVSFYTDIIKVGRTSVTVKVEVYAE
                     RLTKLCNNIAKITEAELVYVALGEDKKPITLEESRARFAQCCSLETGDSSSCS"
     gene            4869..5129
                     /locus_tag="PK077_00425"
     CDS             4869..5129
                     /locus_tag="PK077_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22797.1"
                     /translation="MDRNHESKTKKVDGEAHPASDFAYVPDPEKPSTWKLPIFNMRHV
                     GGALAAMTSDYRGNPVEIPEGDRRTVMKKILARKAKLEQEES"
     gene            5678..6592
                     /locus_tag="PK077_00430"
     CDS             5678..6592
                     /locus_tag="PK077_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRN22798.1"
                     /translation="MTDEKMSTEGTNEIQDPRGEFQQERYEKQQQKAPGLQSGMKPVP
                     DSGEQSYHGCNRLRGRKALVTGGDSGIGRAAAIAYAREGADVALNYLPEEQSDAEEVA
                     ELIRKEGRKAILLPGDLSDEVFCKKLIRDALEKLGGLDIMALVAGKQVAVEDIRDITT
                     EQLTKTFEVNVFSLFWIAKEALPHLKPGTSIITCSSIQAYQPGKNLVDYASTKAAIIA
                     FSRSLAKQVAPKGIRVNVVAPGPIWTALQVTGGQLQKDLPEFGRKTPLKRAGQPVELS
                     GLYVFLASQESSFITAEVFGVTGGMHLA"
     gene            6822..7787
                     /locus_tag="PK077_00435"
     CDS             6822..7787
                     /locus_tag="PK077_00435"
                     /inference="COORDINATES: protein motif:HMM:NF017263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HRN22799.1"
                     /translation="MVHGSPLNAPVITLGEKKSLFGEKAQANEYVSYALYYWPDPANP
                     DGPYKPIDGKKNKRLRSMDDSGRMAAFISTVCSLGRQYKLDRDPQAASRAGQWLKAWF
                     IAPATRMQPHLKYAQIRPGNRTEGGGGGIIDLYRIPEFLEALTALKCSNALTAEEWKQ
                     VDAWLNQYYTWLTTSRQGRHAHTCSNNHYLFYVAQCAHLASFLGRSHEAAQWLAEAFA
                     NMDRHIAPDGSQPEELKRAEPWQYSVYTLQAWGYLVHLAERMGDKNYRYRKTPAGASI
                     QKAVDYLLPYHANPETWPWQGSRANTPLPAIFEPEQEGRPQRKCS"
     gene            complement(8045..8428)
                     /locus_tag="PK077_00440"
     CDS             complement(8045..8428)
                     /locus_tag="PK077_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22800.1"
                     /translation="MHPDIFWIISFFMKWLLIFLISPCMAFAESIRGVVINVIDGDTV
                     ILLEKTPEQMRTHRIRLEGIDTPERGQDGDEGAKKYLEKLIWGETVTVRYTENDRYGP
                     YFRGNMAWKNVRQRRDGEGRMGVDL"
     gene            8478..9002
                     /locus_tag="PK077_00445"
     CDS             8478..9002
                     /locus_tag="PK077_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22801.1"
                     /translation="MNAVICGQPVLEQAIMFSGMKEHNSRTYSNLSGTTVNRLYHCPA
                     PEYLYSLPSRERKNIPPAHPTIQSCCHLEKLHVKQEEPGALQHSMAWREAFHSFFQFF
                     GYSAEVLFRYLSQFLLLFPVPCRSFRHPGLIRQPAHGAARVTACFLLNDFQQLSDIFI
                     HGFRVDIISIPMPG"
     gene            complement(8977..9492)
                     /locus_tag="PK077_00450"
     CDS             complement(8977..9492)
                     /locus_tag="PK077_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044260 - cellular macromolecule metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRN22802.1"
                     /translation="MKTSYQSPVYKIRRVPLDQIRSNAYNPNSVAPPEMKLLYQSIKE
                     DGYTMPIVCYKLEDGTYEIVDGFHRCQIMKTYRDIYEREGGMMPVAVIDKPLGNRMAS
                     TIRHNRARGSHNIALMTNIIQELTEAGMSDAWILKNLGMDAEELLRLKQLSGIASLFR
                     DEEFSRAWESK"
     gene            complement(9489..10784)
                     /locus_tag="PK077_00455"
     CDS             complement(9489..10784)
                     /locus_tag="PK077_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HRN22803.1"
                     /translation="MKTYLDKNVYEAALERIAYCFQEFDNVLVSFSGGKDSGVMLNLC
                     YRYAAEQGLLDKLSMYHLDYEAQYQMTTEYVTRTFLEQFPGIRKMWYCVPIRAQSACS
                     LGEPYWTPWSAAQKKLWVRPMPENPYVINEKNLDVPFRHGMVDYEFQDKLSRHFAKKH
                     GSTAVMVGLRADESLNRYAAVARGNKRTSYEGRKWITRADAVTVSAYPLYDWRVSDVW
                     TANARFGFDYNRLYDLLYQAGLTIGQMRVASPFNDCAQESLKLYKVIDPANWARMVGR
                     VNGVNFTGLYGGTTAMGWKTIKLPPGHTWKSYYEFLLSTMNEKTAEHYNNILERSKKY
                     WMKGGTVDPGTADEVLAAYPLATVTGKSGRYVDRDVIQFMGYPDDMPVSNFRQVPSYK
                     RMCICIMKNDYFCKYAGFGPTKGAIARRRAAVNKYRNIL"
     gene            complement(11680..11754)
                     /locus_tag="PK077_00460"
     tRNA            complement(11680..11754)
                     /locus_tag="PK077_00460"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11720..11722),aa:Asn,seq:gtt)
     gene            11921..13030
                     /gene="leuB"
                     /locus_tag="PK077_00465"
     CDS             11921..13030
                     /gene="leuB"
                     /locus_tag="PK077_00465"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HRN22804.1"
                     /translation="MSEHHHHIAVLAGDGIGPEVMAQALKVLDAVSGKFGFTVSRKEA
                     FVGGAGIDHCGKALPEETIRACEEADAVLFGSVGGPKWEHLPANEQPERGALLPLRKH
                     FGLYANLRPGVCLPALTHASPIKNELIEGGFDILCVRELTGGLYFGQPRFREREGDDE
                     VVVDTMRYHKSEMVRIAKVAFEAARGRRKRVTSVDKANVLTNSLLWRETMIEVSKDYP
                     DVELLHMYVDNAAMQLVRNPRQFDVLVTENLFGDILSDEMAMICGSLGMLPSASLCQG
                     AQDNGLFFGLYEPSGGSAPDIAGKGIANPIAQILSLSMLLRYSLGEKTAADAIDSAVR
                     RVIDQGCRTGDLATGAPGEIRVNAAEMGDAIIAAL"
     gene            13163..14203
                     /locus_tag="PK077_00470"
     CDS             13163..14203
                     /locus_tag="PK077_00470"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRN22805.1"
                     /translation="MNILIIGQGIAGSCLAWELKRRGAEFTVADRPVAETASRVAAGL
                     VNPLTGRAFRPGWRQEECLAAAEAFYPETERELGGSWWQKTPIFREVETEDQFEIWQE
                     RQTAPESCAYAGPLFPWPEHWEGRGKAAYTRGSAVLHVENMVTAMRRFFMEQGRFVEA
                     DVSPADIRPDADGLFHWNGRTFSHIVWCTGWEAGLHPDMSPLKGRPSKGTILDLDLKE
                     LDWHAGILHFGHWLVHNGSFWRFGATYAWAWDAPGIPEAPAVQELMLDLVKRYSGNMD
                     VIRARAAVRPIIRRSQPVAGPIPGLDGQFVFSGLGSKGVTTSPWSAAQLAGHLVCGTD
                     LPPDLLPAALWK"
     gene            14249..15163
                     /locus_tag="PK077_00475"
     CDS             14249..15163
                     /locus_tag="PK077_00475"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRN22806.1"
                     /translation="MSKGSERDYMRPNMRAENENWGDTVRNHLFDQRSAVMVHWLILL
                     NVVIFILGFFFQVDIPRNIYPPVHLDLIQLYGAYSEYTCFHEGELWRLLTYQFLHANL
                     GHIMFNMIALWFFGPVVEERFGHWRFLLYYLFCGVAAALFSSLLGYMGFFDPEWRFIP
                     MVGASGSIYGIMAACAVLFPHARVQLLFPPVNLSVRQFALAVLGVAAAVIIFQWNNAG
                     GEAGHLGGMFAGFILTLLILWKEKMSSPRRKAASSSHYSPEPSSRRPAQAYPSEKEVD
                     EIMEKISRSGLSSLTEEEREILRRASRR"
     assembly_gap    15319..15375
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15376..16816
                     /locus_tag="PK077_00480"
     CDS             <15376..16816
                     /locus_tag="PK077_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRN22807.1"
                     /translation="FIVGLPDAAVKESGQRVDTAIQNSGLPFQQGIFVVNLAPADLRK
                     QGPGFDLPIALGMVAGAAEKEMDASAWCIVGELALDGTVRPVQGILPQIMEARRMGRK
                     RIMLPQANAYEGTPVQGVEIYPVSSLREAWHLLTSDALPPPFTGSGREAHAPRDKDAA
                     VDFDEIKGQPYARRAMEIAAAGGHNILLCGSPGSGKSMLAQRLPTILPPLSPEEALET
                     SKIHSVCGLLKRGNGLVDQRPFRAPHHTISDAGLMGGGANITPGEVSLAHNGVLFLDE
                     LPEFRRAALETLRQPLETGQVVISRASGTMTFPCRFMLAAAMNPCPCGYLGDRRRACT
                     CPPAQIARYRRKISGPLLDRFDLLMEVPAVDPSILASAPAGECSASIRERVAAARRLQ
                     SSRYAGTPFRNNAALSGKALQKYCRLLPEGRAILLRAVEELALSARAYDRILKVARTI
                     ADLEGTSDIQDVHLYEAVQYRAFEQSLRD"
     gene            complement(16929..17510)
                     /locus_tag="PK077_00485"
     CDS             complement(16929..17510)
                     /locus_tag="PK077_00485"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN22808.1"
                     /translation="MARTSGQELLDKVKGLQNGIYAGGNNNSVGNWSVNGEGTWVNQD
                     DVGSITLCGRSGVAGSSFAMVLGGPQEGDSVSSITFSCNTPSSVISSYSMVLAVYDST
                     GTLVEELSKIQDFSFTSTSETTTISLDMSAAPLTWEEGYKVVAGVRGGAGGATSAYTI
                     TGIQVSYETVPEPAAASLGLLGLGAMLLRRRRS"
     gene            complement(17870..19159)
                     /locus_tag="PK077_00490"
     CDS             complement(17870..19159)
                     /locus_tag="PK077_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRN22809.1"
                     /translation="MNQELRPETLCVQAGWAPKKGEPRVLPIYQSTTFKYETSEQMAK
                     LFDLEEAGFFYTRLQNPTNEAVARKIAELEGGVAAILTSSGQAASFFAIFNICEAGDH
                     IVSASAIYGGTYNLFAVTFRKMGIEVTFVDQDAPAEEIAKAFRPNTKAFFAETISNPT
                     LCVLDIRKMADIAHAHGVPLIVDNTFATPINCRPFEWGADIVTHSTTKYMDGHAAAVG
                     GVIVDSGNFDWEAHKDKFPGLTEPDPSYHGLSYSKSFGKGAYITKATVQLMRDLGSIQ
                     SPQNAFLLNLGLETLHLRVPRHCENAQKVAEFLQGQEDVAWINYPGLSSNEYYELAQK
                     QFRCGQSCGVVTFGIKGGRERSIKFMDSLKLAAIVTHVADSRTCVLHPASHTHRQLTD
                     EQLMEAGVRPDLIRFSVGTEAAEDIIADLKQALEAIR"
     gene            complement(19400..20647)
                     /locus_tag="PK077_00495"
     CDS             complement(19400..20647)
                     /locus_tag="PK077_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRN22810.1"
                     /translation="MINITRLWTGAEQPADHLRYGQGHGHGRFAGGAAESCAPASSRV
                     RKPIVVWNITRTCNLKCIHCYADASARKFEGELDWDQCCAVIDDLADYKVNALLFSGG
                     EPLVHPRFMELLERATGKGLKVTISTNGTRITPEAAARFKELGVAYVGISLDGIGAVH
                     DKFRGVEGSFEQAVRGFRLCSEVGQKTGLRLTLTRNNVQCMGQILDFIDANDIQRVCF
                     YHLVPTGRGVEVQTLTQEEARNAMDTLIARVEEWKAEGKNREVLTVTQPADGIYLLLR
                     QLREGSSLAKETLGLLQWNGGGANSSGRGIANIDTQGAVHPDQFWQSVTLGNVKSDRF
                     SDLWDARAGAAAEMLPELRGSDDPLERQKKIEGRCGRCVHFALCGGGFRTRAAFANGH
                     WYGSDPGCYLTEEEISIPLPEIK"
     gene            complement(20640..21701)
                     /locus_tag="PK077_00500"
     CDS             complement(20640..21701)
                     /locus_tag="PK077_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRN22811.1"
                     /translation="MRPVFSETDLNERPFLVFWEVTRACALACRHCRAVAQPRPHPDE
                     LTHEEALRLIDQLAELRPPMLVLTGGDPVMRPDILELIRAASGKGLHVALSPAATARL
                     LHTDFRALKEAGVQSMSLSLDGAHESTHDAFRGVPHTYERTLRAAEMAKEAGMHLQIN
                     TTITRSTLGEFDDFVELMKKMKPDMWSVFLLVPTGRAAMDEMPAAEEVEAVWKKLSRI
                     SHEVPFGVKTTEGHHYRRVALQEARAQGSKPARRAIPTRDGKGVMFISHTGEIQPSGF
                     LPMTAGNVRTDDPGEIYRTHPLFLRLRDDNALRGKCGRCEYRAICGGSRSRAYAVYGD
                     MMAEDNLCPYQPRLSQHHD"
     gene            complement(21773..22591)
                     /locus_tag="PK077_00505"
     CDS             complement(21773..22591)
                     /locus_tag="PK077_00505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22812.1"
                     /translation="MNKIVASISLGTAVCITASAFAQDSHLSLTRQSVASMHEIAAAL
                     DKVTDEPGAAAAVETVKKVHKKLLDIGKRQLELPGATLQEQAQVSAQMTEAQKISGEL
                     IKSLSRLKKEGLLTPDLQKALADLQSVQKELAQASRDKAEQAEPFPEDAEGNTHETLL
                     TRTVEEVKKLNDSLARVRDEETGKAAVEQIGRYRAAMLDVARKQAALPPLTRNQQAKL
                     MGVQQQLVEMMQPIVKHVTAIMLSPDASRNLKEALNSLSELPKEVQKIREEAAK"
     gene            complement(22733..23167)
                     /locus_tag="PK077_00510"
     CDS             complement(22733..23167)
                     /locus_tag="PK077_00510"
                     /inference="COORDINATES: protein motif:HMM:TIGR04073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase system-associated protein, TIGR04073
                     family"
                     /protein_id="HRN22813.1"
                     /translation="MKRAIISALFAAGVVGVASADIQAPPASEYTPTRKLGRAIANLI
                     YSVEEIPLGIINWTSREGDYAGFSVGIVDGTATMFERMGYGIYELVTFWAPTYKCTYK
                     PPYQGRCGMSGLKEYNPNGGLSEFPAELSFQSYYKYSRQQRD"
     gene            complement(23225..24235)
                     /locus_tag="PK077_00515"
     CDS             complement(23225..24235)
                     /locus_tag="PK077_00515"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HRN22814.1"
                     /translation="MMNRLHKIAVIGAGSWGTALSMVLATRECEVVLWTPEEAQARQL
                     AETRRSPVLSKTALPLAPNIHPTCDLGQVKDAELIVVVVPSVAMRSVAQRLCSLPVQP
                     DAVIVSCTKGIEQGTHKRMTEILQEYLPSNPIGVLSGPNHAEDICLGLPSASLIGFED
                     PKYADWVQQIFASKTFRVYSATDIIGMQLGGTIKNVFAIGAGLCEGLNLGDNAQAALL
                     TRGLAEMTRIGVASGGRRETFMGLSGVGDLIVTCYSRHSRNQTVGRRLAEGKSLQEIL
                     DTLGMVAEGVPNTLSVYEIARKLEVRTPLIDAVYSVLYESKPPMEALAELMTRDPRPE
                     LD"
     gene            complement(24237..24881)
                     /gene="plsY"
                     /locus_tag="PK077_00520"
     CDS             complement(24237..24881)
                     /gene="plsY"
                     /locus_tag="PK077_00520"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRN22815.1"
                     /translation="MIAWIVFYYLIGAIPFGYLAGRIRGIDLRQQGSRNIGATNAWRV
                     LGWKWGLSVFILDFLKGFIPVFGALNWLPFLTGDAGGWDFNVLIVLSCFAVILGHTYT
                     CFLGFRGGKGVATTAGVLFALNPVVACVALASWLVVMGISGIVSLASLVAAVVMIAAG
                     WWIYPLCQDGDVSPQILYVAFFTVVGALVILKHRSNIARLLNGTEHSFYSKKSK"
     gene            complement(25134..25919)
                     /locus_tag="PK077_00525"
     CDS             complement(25134..25919)
                     /locus_tag="PK077_00525"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRN22816.1"
                     /translation="MAPPTPDILDCASAQWIGKRREQEDVVKSLPAAGGMLGIVCDGM
                     GGHLRGDCASCVVAQEFAAVFAESDIEDIPERLTAALHAANRALAAIQKEPEESGTTL
                     LAVFIRDHFLWWISVGDSPLYLWSGADSFRMDRLNEDHSMRPIADCLYRKGKMSLQGA
                     LRQRCILRSAVMGGPMELVDISAMPLLLHPGDAVLACSDGLQVWSELLREPSFLALQK
                     ARDCSSRELVGIIMEQVKDMLEPQQDNASVWAAVMRKPCEGGK"
     gene            complement(25937..>26961)
                     /locus_tag="PK077_00530"
     CDS             complement(25937..>26961)
                     /locus_tag="PK077_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRN22817.1"
                     /translation="VTYRKRHLDLISNEESASLFVTRSLMIAEIRRFLQERGYLEVET
                     PMLQDVAGGAAAKPFETYHNALGMPLTLRIAPELFLKRLMVGGFTKIFELNRSFRNEG
                     IDRRHNPEFTMLEAYCACGDFETMANMVEELICHLAEKFCGGLQIDHKDAEGNVLYTI
                     DLSRPWKRADYQDLIRGVAGEDWFDISPEARRARCEELGVEISPDMKDVDVSQQVYEK
                     LVEEKTMNPCFVTHVAKDLVPLAKLNRENPDVVDVYELVINGQEISPGYSELNDPDVQ
                     KERLEHQAAGETQRVDYDFIETLEYGMPSAGGIGIGIDRVVMMLTGASSIRDVLLFPQ
                     LKRKDS"
BASE COUNT         5976 a         7800 c         7015 g         6086 t
ORIGIN      
        1 aagggagtgc cggacagcga acttccgcga acggaatcac tgaaagaaac tattgcccgc
       61 atgatgccct actgggaagg aacagttctc ccttctctac ggacgctgga caatatcctg
      121 atcgtagccc acggcaacac attgcggggc atgatcaagt atttgaaaaa cattcctgac
      181 gaacagctgc tttcccttaa tcttcctacc gccaccccct atgtatttga attttacgac
      241 tccctgaacc tgggaaaaga ctactttctt ggggacccgg aagaaatccg caaacgcatg
      301 gcggccgtcg ccggccaggg aagcgcaaag aaaaaaacgc ctgcggaata acctcactat
      361 cctttcctat gaccgtccat tcctgccgca ttgcgttctt tgacgccaag ccctacgacc
      421 gggattcctt caattctgtc aatgaacgcg aattccatta cgacatccgt tacttcaagg
      481 ggcacctcac tccggacagc gtgcccctga cccgcggtac ggacgtagcg tgcattttcg
      541 tcaatgacac ggcaaaccgg gaagtaatac ggaacctgaa ggaaaatggc gtcaaattgc
      601 tggctctgcg ttgcgccgga ttcaataatg tggatctgaa agctgcggaa gaagcggaac
      661 tgcccgtcgt gcgggtgccc caatattctc cctatgcggt ggcggaacat gccgtagccc
      721 tgatgctttc cctgaaccgc aaaatacacc gcgcctactg gcgcaccagg gacggcaatt
      781 tttctcttca cggcctgatg ggctttgaca tgaatggaaa aacggccggc atcatcggaa
      841 cgggaaaaat cgccagaatc ctcatccgca ttctcaaagg tttcgggatg aacatcctgg
      901 cctatgacct gcaccctgac cagcgctttg cggaagaagc cggcattacg tacaccaccc
      961 tggacgatct gtacgcccgg tccgatatca tctccctgca ctgccccctt actccggaaa
     1021 cggaacacct gatcaatacg gattccatcg gaaaaatgaa ggatggagtg atgatcatca
     1081 acacgggacg cggcaagctg attaacacgg aaatgctgat tgacggcctg aaatcaaaaa
     1141 aagtgggagc tgccggactg gacgtgtacg aagaagaggg agaatatttc tacgaagaca
     1201 aatccgacag gattattgat gacgatactc tggcccgtct cctttccttc aacaacgtca
     1261 tcctgacttc ccaccagggc tttttcacga aagaagccct gcacaatatt gcggaagtca
     1321 ccctnnnnnn nnnncaacat ccgggacttt ctggaaagca aacctctgat caaccgagtc
     1381 tcgcttcagt cacgctgaac gtgcccggcg ggcgctctac ggaaatccgg gactttctca
     1441 tccgccttct gccggacaga aggcggaatt cggggaaagg cggcagcttt tttatgcaag
     1501 cgccgccact tcccgccgta ttatatacta tgagcactcc agcccctgcc tccgtcctgg
     1561 ctcccaaacc gcactacgcc gctctggacg gcctccgcgg agtggccgcc gtcatggtgg
     1621 tcctgttcca catgtttgaa ggcagcgcca gggacttcca gttccacatg gaccagatca
     1681 tcaaccacgg ctacctgtcc gtggacttct tcttcatgct gtccggcttc gtcatcggat
     1741 acgcctatga cgaccgctgg gaaaaaatga acctgtggaa cttctgcaag cggcggctgg
     1801 tgcgccttca acccatggtg gtcatgggga tgctgctggg gggaatcctc ttttacttcc
     1861 aggggtctga aatcttcccg aaccttgcgc aaacccccgt atggaaaatg ctgctggtca
     1921 tgaccgtggg attcacgctg ctgcccgttc ccgtttccct ggatgtgcgc ggatggtcgg
     1981 aaatgcaccc gctgaacggt ccggcctggt ccctcttctt cgaatacgtc gccaatatcc
     2041 tttatgccat gctcatcaga aaatttacca gcagatggct ggcggtcctg gtctttctgg
     2101 cgggctgcgc cctcattcac cagtgctaca cgcagggcaa caacatcggc ggctggactc
     2161 tggatgcgga acagtgccgt gtggggctta cacggctcat gttccctttc ttcggcggtc
     2221 tgctcctgtt ccgcctgtgc aaacctggac ggatcaggca tggtttctgg tggtgcagcc
     2281 tgcttctggc gggaaccctg gcggcgcccc atgtaggagg cgcggaacat ttccgcctga
     2341 acggcctgta tgacgccttc tgcatcattg tcatctttcc cgtgatagtc ttcctcggag
     2401 ccagtgacat ccctggagac atctcttcca gagccagccg gtttttggga aacatctcct
     2461 atccccttta tatcacccac tatcctatcg tatatctcta cctggcctgg gttcacgatg
     2521 cggaagtccc tctttcccgt gcgctccccg tagcggcagg cgtcttattc ctctgcattg
     2581 ccgtagccta cgggtgcctg aagctttatg acgagcccgt gcgcgcatgg ctttccaaac
     2641 gcgtgctgaa gcggcatcct ccggaacccg gggcgtaaag cggaacaaac atctgccttg
     2701 ccccgggcgg ggccaggccg gatactgtca gcggtctttc atggaaaaac tgccgacatc
     2761 ctccaaagct ctgcgctacc tgccctttct tgtggcatcg gcattcttta tgcagatgct
     2821 ggatgccacc atcctgaaca cggccattcc caccattgcc cgcagcttca acagccatcc
     2881 gctgcagctc cactccctgg taacggccta catgctcacc gtctgcgtcc tgatgccggc
     2941 ctccggatgg atatnnnnnn nnnnnnnnnn ncgccgtact ttcctgctgg ccatctccgt
     3001 tttttccgta ggctccctgc tgtgcgccct ttccgcctcc gttaccatga tgacggtatg
     3061 ccgcgtcatc cagggaatag gcggcgcctt cctgatgccc gtcgggcgcc ttgtcatcct
     3121 gcgctcctat ccccgctcca tgttcgtcaa tgtcctgaac attgtaacga ttccggcgct
     3181 gctgggcccc cttctggggc cggtgctggg cggtatcatc gtacagtacg cttcctggca
     3241 ctggatattc ctgatcaaca tcccggtagg catggcgggc ctgtgggcca cctggaagct
     3301 gatgccggac ctgaaagcgg tccgggaaca gaaattcgac tggccgggtt tcttcctttt
     3361 ctcctcctcc gccgtcctgg ctacgatggg cctctcttcc gcaggaggca taaccgataa
     3421 gccgcgcatg gtcatcctca cgtctgccgg aggcattctt caacttctgt actgggtgct
     3481 ggcgttccgt tcaaaagccc ccatcttcag cccggccctg ttccgcatca gaaactttgc
     3541 catcggaatc gccggcaaca tcgtctgccg cctgggcgga agctgcctgc cgtacctgat
     3601 gccgcttttc ttccaggtgg tgcttggtta ttccgctctt cagtccggca tgtccctgat
     3661 tccgctggcc ctgagcaatc tgctggcaaa aacggttgcg ccgcgtctgc tcgggaaatt
     3721 tggttaccgg aacatcatgg tcgtcaatac cttcaccata ggagcgctgc tggcctcttt
     3781 ccactttgta ggccccggaa cccatgaact tattctcctg ggcatgctcg cgctgctggg
     3841 cggggccaac tccattcagt tcacctgcat gaacaccctg acgctgattg acctccccaa
     3901 tgcagacgcc agcagcggaa actccctgct ttccacgatc atgcagctct ccattgctgt
     3961 cagtattgcc gcggcctccc tgctgctgga cagcttcggc ggccattcgg ctacctccgg
     4021 aaaggctgtg gaaaccgcct ttcataccac tttcgccacc atcggcgcca ttgccgcggc
     4081 cagttccttc atcttcgccc tggtggacaa agacaaggga atcacccgca aacggaaaaa
     4141 agcagcttga agaggaaaat tcccgcccct gttcaggaac aggaagagga atctcccgtc
     4201 tcaagagaac agcactgcgc aaaccgggcg cgggactctt ccagggtaat cggtttctta
     4261 tcctccccca gggctacata aaccagctcc gcctctgtaa ttttggcaat gttattgcaa
     4321 agcttcgtca gacgttccgc atatacctcc accttcacgg tgacggaggt gcggcccacc
     4381 ttgataatat ccgtataaaa ggacactaca tcgcccacca gcacaggagc ttcaaacgtc
     4441 aggctgctca ccgcacgcgt cacaatatag cggctcaatg catagcgcat ggcggtattg
     4501 gctcccgcaa ggtccacctg gctcatcacc cagccgccga atacttcccc gctctgattg
     4561 gtatccgcag gcattggttc cacgcgcagg gcgggaacgc gcccttccgg aagctgggca
     4621 tgacgatatg tattttccat aaataagaat tattataaat tgataaggac atgcttatct
     4681 atcacagccc tcttgtaaag ggtaaaacgc ggaagcggaa aacgggcggt gccggcacgc
     4741 tctgcggaaa aaagaaaaca gcccggaata aactgctttc ccatattgat taagttgggc
     4801 aggcggattt ccgtgtctta tcccggagct tttccaccat ggccggtaaa ggcggttgtc
     4861 cattactcat ggaccgcaac catgagtcca aaacaaaaaa agtggacggg gaggcacatc
     4921 ccgcttccga ctttgcctac gtacccgatc cggaaaagcc ttccacctgg aaactgccga
     4981 ttttcaacat gcgccacgta ggaggggccc tggccgccat gaccagcgac tatcgcggca
     5041 accccgtgga aataccggaa ggagatcgcc gtaccgtcat gaaaaaaatc ctggccagga
     5101 aagcgaaact ggaacaggaa gaaagctgat tccgttttct ttcatttctt tttttccgcc
     5161 tgtaaacagg aaggaaagaa atatcttttc ccttcgttcc ccggtcttag ccgtttgggg
     5221 acgggctgac accatccaga taccggcttt ctgcggcgcc ttcggctggc acccccgtag
     5281 aaatgaaaga cattcccacc cttccgacgc tcctggcgga tacagccgga tatttcactt
     5341 acggacggca tcaaaaacgg tggtaaaaca gaagaagaaa ttaaattatt ctaaaatcat
     5401 caatcatccc aaggatgttt ttcccctaaa gtggattggg gaaattcagg aaatctgaaa
     5461 atcaaataac tttctccggc caataaaaac aacatttaga aaattcctat ataaataaat
     5521 atcaatggca tattgaaata aaaacatgtc tttccctgaa caggctgtcc gaagcctggt
     5581 catcacgaca ggaatgaaga atgccggaaa agggaatgct gccggctatt tcccgcatgg
     5641 cagcgctttt ccccttccgt caactccgca tcaaaatatg actgacgaaa aaatgagcac
     5701 agaaggcact aacgaaatac aggacccgcg cggtgaattc caacaggaaa gatatgaaaa
     5761 gcagcagcag aaagcccccg gactgcaatc cggaatgaag cccgtgccgg attccgggga
     5821 acaaagctac catggctgca acaggctccg tggacgaaaa gccctggtca cagggggaga
     5881 ctcgggaata ggccgggcgg cggctattgc ctacgcacgc gaaggagccg acgtagcctt
     5941 gaactacctt cctgaagaac aaagcgatgc cgaagaagtg gcggaactca tcagaaaaga
     6001 gggccggaag gccatcctac tgccggggga cctcagcgat gaagtctttt gtaaaaaact
     6061 catcagggat gccttggaaa aacttggcgg actggacatc atggccctgg tagccggtaa
     6121 acaagtggcg gtggaagata tccgggacat taccacggaa caattaacca agacatttga
     6181 agtaaacgtg ttctccctgt tctggattgc caaggaagcc cttccgcatc tcaagccggg
     6241 gacgagcatc attacctgct cctccattca ggcctaccag cccggcaaga acctggtgga
     6301 ctatgcttcc acaaaagcgg ccatcatcgc gttcagccgc tccctggcca agcaggttgc
     6361 ccccaaaggc atccgggtca acgtggtggc tcccggcccc atctggacag ccctgcaagt
     6421 aaccggaggc cagctgcaga aggacctgcc ggaattcgga cggaaaacgc ctctcaaaag
     6481 ggcagggcaa cctgtggaac tctccggact atacgtcttt ctcgcctccc aggaatccag
     6541 cttcattact gcggaggtct ttggcgttac cggcggcatg catctggcct gatgcggaaa
     6601 cccgcaatcc gggaaaaggg ccggactgac tttccggaat ctctttccgc agcgccacac
     6661 ccccggatga ccgggaaagc ggccggaaac ggctctgcaa ggaaatcagg gcattgctcc
     6721 atttcccggt tgtcccggca aaaggttcct gcttgaatag ggtaatggca ttttggaggt
     6781 tttggttcat ctttttccct ttcacggcgc ttctcttttc catggtgcat ggcagtcctc
     6841 tgaacgcacc cgtcatcacc cttggagaga aaaaaagcct gttcggagaa aaagcgcagg
     6901 ccaatgaata tgtcagctat gccctttatt actggccgga ccccgccaat ccggacggtc
     6961 cttacaaacc cattgacggc aaaaagaaca aacggctccg cagcatggac gacagcggca
     7021 ggatggccgc tttcatcagc acggtctgca gcctgggacg gcagtataaa ctcgacaggg
     7081 atccgcaggc cgcctcccgg gccgggcaat ggctgaaagc ctggttcatt gctccggcaa
     7141 cccgcatgca gccccatctg aaatatgccc aaattcgccc cggaaaccgg acggaaggag
     7201 gcggaggagg catcatcgac ctttaccgca ttcccgaatt cctggaagct ttgacggcgt
     7261 tgaaatgctc caatgcccta acggcagagg aatggaagca ggtggatgcg tggctgaacc
     7321 agtattacac atggctgact accagcaggc agggcaggca tgcacacacg tgcagcaaca
     7381 accattatct gttttatgtg gcgcaatgcg cccatttggc atccttcctg ggccgcagtc
     7441 atgaagcggc ccaatggctg gcggaagcct tcgccaacat ggaccgccac atcgctcccg
     7501 acggatccca gcccgaagaa ctcaaacggg cggaaccctg gcaatacagc gtttacactc
     7561 ttcaggcatg gggatacctg gtgcatcttg cagaacgcat gggcgacaag aactaccgtt
     7621 accggaaaac acccgccggg gcttccattc aaaaagctgt ggattatctc ctgccttacc
     7681 acgccaaccc tgaaacatgg ccctggcagg gctccagggc aaacactccg ctccccgcca
     7741 tctttgaacc ggagcaggag ggcaggccac aacggaaatg ctcctgaacc gcgcccgccg
     7801 ttaaaagcat ccgtttttcc tgcttattga aaatcccctg tccagcagca tcacgaacga
     7861 ggcattttcc cggaaaacga tttaataagg gatctcccca gtatttttcc ccttagattt
     7921 ttcctttctt tttgctgctt tccattccca cggagatatg ggattcgggt cagaccagat
     7981 gcctttttgg gcctttctgg catccctttc aagagaaacg agcttccggc tggcggaaaa
     8041 atttttataa atccacgccc atccttcctt caccatctct tcgttgacga acatttttcc
     8101 atgccatatt tcccctaaaa tacgggccgt acctgtcatt ctcggtatac cggactgtaa
     8161 ccgtctctcc ccaaatcagc ttttccaggt atttcttggc gccttcatcc ccgtcctgcc
     8221 ccctttccgg cgtatcaatt ccctcaagcc gtatccggtg cgtacgcatt tgttcaggcg
     8281 tcttttccag aaggatgacc gtgtcgccgt caatcacgtt aatcacgact cccctgatac
     8341 tttcagcaaa agccatgcag ggacttatca ggaaaatcaa aagccatttc atgaaaaatg
     8401 atataatcca aaaaatatca ggatgcaact actctttaga gaaaccgttc ccgcactgtt
     8461 cacacggccg gaaacagatg aatgcggtca tctgtggaca gcctgttctg gaacaggcca
     8521 tcatgttttc cggcatgaag gaacacaatt cccgaaccta ttccaacctg tccggaacaa
     8581 cggtaaacag gctgtatcat tgccccgctc cggaatatct ttattcgctg ccgagccggg
     8641 aacggaaaaa catcccgccg gcgcacccaa caatccaatc atgctgccat ttagaaaagc
     8701 tgcatgttaa acaagaagaa ccgggcgccc tgcaacattc aatggcatgg cgtgaggcat
     8761 ttcactcatt ttttcaattc ttcggctatt ctgccgaggt tcttttccgt tacctttctc
     8821 agtttctcct gctgtttcca gtaccatgcc gttctttccg acaccccggc ctgattcgcc
     8881 agccagcgca cggcgccgct cgtgtcacag cctgttttct tctgaatgac ttccagcagt
     8941 tgagcgacat atttattcat ggattccggg ttgatattat ttcgattccc atgcccggct
     9001 gaattcttcg tcccggaaca gggaggcaat cccgctgagc tgcttgaggc gcaacaattc
     9061 ttccgcgtcc atccccagat ttttgagtat ccacgcatcc gacatgcccg cctccgtcaa
     9121 ctcctggata atattggtca tgagggcgat attatgagat ccccgggcac ggttgtgcct
     9181 gatggtggaa gccatgcggt ttccaagagg cttgtcgatt acggccaccg gcatcatgcc
     9241 accctcccgt tcgtaaatat cgcggtaagt cttcatgatc tggcagcggt ggaacccgtc
     9301 cacgatttca taggtcccgt cctccagctt ataacaaacg ataggcatcg tataaccgtc
     9361 ctccttgatg ctctggtaga gcagcttcat ttcgggggga gctacgctgt tcggattgta
     9421 ggcattggaa cgtatctggt ccaggggaac gcgccggatt ttgtaaacgg gggattgata
     9481 ggaggttttc ataaaatatt gcggtattta ttgacggcgg cccggcgccg ggcaatcgct
     9541 cccttggttg gaccgaaccc ggcgtacttg caaaagtaat cattcttcat gatgcagatg
     9601 cacatgcgct tgtaggaagg cacctgccgg aaattgctga ccggcatgtc gtcgggatag
     9661 cccataaact gaatgacgtc gcggtcgacg taacggcccg actttcccgt cacggtcgcc
     9721 agaggatagg cggccagcac ttcatcggca gttccgggat cgacggtgcc ccctttcatc
     9781 cagtactttt tagacctctc caaaatgttg ttgtagtgtt ccgccgtttt ctcgttcatt
     9841 gtggaaagca ggaattcata ataggacttc caggtatggc cgggcggcag tttgatggtt
     9901 ttccagccca tggccgtcgt acctccgtac aaacccgtga agttgacgcc gttgacgcgc
     9961 ccgaccatgc gcgcccagtt ggcaggatca ataactttgt aaagcttcag gctttcctgg
    10021 gcgcagtcat taaacgggct tgccacccgc atttgcccga tcgtaaggcc tgcctggtac
    10081 aggagatcgt aaaggcggtt atagtcaaaa ccaaaacggg cgttggccgt ccagacgtcg
    10141 ctgacgcgcc agtcataaag agggtaggcg ctgacggtca cggcatcagc ccgtgtaatc
    10201 cacttccttc cctcataaga cgtcctttta ttgccgcggg caacggcggc gtatctgttg
    10261 aggctttcat ccgcccgcag ccctaccatg acggcagtgc ttccgtgttt tttggcaaaa
    10321 tggcgtgaaa gcttatcctg gaattcatag tccaccatac cgtgccggaa cgggacgtcc
    10381 agattctttt cgttgattac atagggattt tccggcatgg gtcgcaccca cagttttttc
    10441 tgggcggcgc tccacggcgt ccagtaaggt tcccccaggg agcatgcgga ctgggccctg
    10501 atcggcacac agtaccacat cttacgaatg ccggggaatt gctccagaaa tgttctggtg
    10561 acatactcgg tcgtcatttg atattgggcc tcataatcaa ggtgatacat ggacagctta
    10621 tccagaaggc cctgttcggc ggcgtaacga tagcagagat taagcatgac gccgctgtcc
    10681 ttgccgcccg aaaaagacac caggacattg tcaaactctt ggaaacaata ggctatgcgt
    10741 tctaaagccg cctcataaac atttttatcc agatatgttt tcacaacttt tccaaagcct
    10801 gtctttcgcc atccgccgcc agccccctgc cggacgggaa cacgtcggcc cggatatgat
    10861 tgccggaagc catgcatttt ccctgaaacc actcttgcag cttgcagctc atagggatat
    10921 cccctgttct ttcaaacagg gttgcaacgc cttcatttct gcataaataa gcaacatcat
    10981 gttttttcat gttttcaaat gcacagcagc ttatcgtccg ttattttccg tcaacaatac
    11041 caacctactg aattattgcg gcgggtcaat gtttttactg caattatttc agtaatttta
    11101 ttgtcatcac cctccttctc aaaatacctg cgctttttag cgagcaacac cattccagaa
    11161 atgccttatg cattgcgcca tgacctcctt tccttctccg caacatccgc gctatgtgca
    11221 gcattcaaga cgctgcgttg gaatgcacgg tttcaaaatc ggagatacaa aaatcataac
    11281 ttgcttaaaa ttaggtgaac gaaaaaatgt acgactcaga gccattttcc ggttccaaca
    11341 tctttccccc aatactcttt ccggaaccgc atcttccagg cagacgatac atccatcatt
    11401 tccttcctga aggaaaaata aacgtgtttc cctttttgca atccgggatg ctgtccaaac
    11461 atgaatatgc cggtacacgc cttaatcatg ccgtcagatt agactttagg cgttttatga
    11521 ttttccccga aacgccaatt tcatgccaga acggaatcaa aaaatgacat ttagtcagca
    11581 cttatggttg tttttccgcg gtttttcacc tttccagcct caaaacagac aaaaaagaag
    11641 ccctgtaatc cattaaaatt acagggctta gaaaattagt ggctcccccg gttgggctcg
    11701 aaccaacgac ctagtgatta acagtcaccc gctctaccgc tgagctacag gggagtttgc
    11761 attccatgga acgcggaggg atttttactg gaggtacggg cgtttggcaa gtactttttt
    11821 actcttgcag gctttttgac gcaaaaaggc ttccgcggcg cggtcatggc aacggtgttt
    11881 tcttgacctt tgaagggaga atgaccagac tttcagcgac atgagtgaac atcaccatca
    11941 tattgctgtc ttggccggcg acggcatcgg gcccgaggtc atggcacagg ctctgaaggt
    12001 tctggatgcc gtaagcggca agtttggctt taccgtaagc cgcaaggagg catttgtagg
    12061 gggtgcgggc attgaccact gcggcaaggc tcttcccgaa gaaaccattc gtgcctgcga
    12121 agaggcggac gccgttctgt tcggctccgt gggcggtccc aaatgggagc atctccccgc
    12181 caatgaacaa ccagaacgcg gagccctgct gcctctgaga aaacatttcg gcttgtatgc
    12241 caacctgcgt ccgggcgtat gccttccggc cttgacccat gcctccccca tcaagaatga
    12301 actgattgaa ggcggattcg atattttatg cgtccgggaa ttgactggcg gcctgtattt
    12361 cggccagccc cgtttccgcg aacgggaggg agacgacgaa gtcgtcgtgg ataccatgcg
    12421 ctaccataaa agcgagatgg tgcgtatcgc caaagtggcg ttcgaggctg cgcgcggccg
    12481 ccgtaagcgc gtcaccagcg tggacaaggc caatgtgctg accaattccc tgctgtggcg
    12541 cgagacaatg attgaagttt ccaaggatta tcccgacgtg gaattgctgc atatgtacgt
    12601 ggacaatgca gccatgcagc tggtgcgcaa tccccgccag ttcgacgtgc tggtgacgga
    12661 aaacctgttc ggggacattc tttccgatga aatggccatg atttgcggtt ccctgggcat
    12721 gctgcccagc gccagtctgt gccagggcgc acaggacaac ggcctgttct tcggccttta
    12781 cgaaccctcc ggaggctccg ctccggatat cgccggcaag ggcattgcaa acccgatcgc
    12841 ccagattctt tccctttcca tgctgttgcg ctactctctg ggagaaaaaa cagcggcaga
    12901 cgccattgac tccgccgttc gccgcgtgat tgaccagggc tgccgcacgg gcgacctggc
    12961 tacgggagct cccggagaaa tccgcgtgaa tgcggcggaa atgggtgacg ccatcatcgc
    13021 cgccctgtaa acagggccgt tcttccggcg ccgcagggga agggacacct gcggcacgcc
    13081 tttcctccgg ggggagcggt accgcctccc ctccgctttc ctctttcatt tccgggattt
    13141 tccggttctt tatccatcta tcatgaacat ccttatcatc ggccagggaa ttgccggaag
    13201 ttgtctggcg tgggaattga aacgccgcgg cgcggagttt acggttgcag accgccccgt
    13261 agcggaaacg gcctcccggg tggcggccgg attggtaaac cccctgacgg gccgcgcctt
    13321 ccgccccgga tggaggcagg aagaatgcct ggcggcggca gaagcctttt acccggagac
    13381 ggaacgggaa ttaggcgggt cctggtggca gaaaactccg attttccggg aagtggagac
    13441 ggaggaccag tttgaaatct ggcaggaacg ccagacggct ccggaatcct gcgcctatgc
    13501 cggacctctt tttccatggc cggaacactg ggaaggccgc gggaaagccg cctatacgcg
    13561 aggttccgcc gtgctgcacg tggaaaacat ggtcactgcc atgcggcgtt tttttatgga
    13621 acagggccgt tttgtggaag cggatgtatc ccccgcagat atccggccgg atgcagacgg
    13681 tcttttccat tggaacggtc gcacgttttc ccacatagtc tggtgtaccg gctgggaagc
    13741 cgggcttcat ccggacatgt ctccgctgaa ggggcgcccc tccaaaggaa ccattctgga
    13801 tttggacttg aaagagctgg actggcatgc cggcatcctg catttcggac actggctggt
    13861 tcataacggt tctttctggc gcttcggggc cacctacgcg tgggcgtggg atgctcccgg
    13921 cattccggaa gcccccgccg tccaggaatt gatgctggac cttgttaaac gctattccgg
    13981 gaacatggac gtcatccgcg cccgtgccgc cgtccgcccc atcatccgcc gcagccagcc
    14041 ggtcgccggt cccattcccg ggctggacgg tcagttcgtc ttctccgggc tgggcagcaa
    14101 gggggtgacc acttccccct ggtccgccgc ccagcttgcc ggacatctcg tgtgcggaac
    14161 ggatctgccg ccggatctgc tgcctgccgc gctctggaaa tgatacggaa catcagctgt
    14221 ccttccttga acggaacagc tattttcctt gtcaaaaggt tctgaacgcg attacatgcg
    14281 gcctaatatg cgagcggaga acgagaactg gggcgatacg gtacggaatc atctgtttga
    14341 ccagcgcagc gccgtcatgg tgcactggct gattctgctc aatgtagtca tctttatcct
    14401 gggattcttc ttccaagtgg acatcccaag gaatatttac ccgcctgtcc atttggactt
    14461 gatccagctt tacggagctt acagcgaata tacgtgcttt cacgaggggg aattatggcg
    14521 gttgcttacc tatcagtttc ttcacgccaa tctggggcac atcatgttca acatgattgc
    14581 cctgtggttt ttcggcccgg ttgtagaaga acgtttcggc cattggcgct tcctgctgta
    14641 ttacctgttt tgcggcgtgg ctgcagccct gttttcctcc ctgctggggt acatgggatt
    14701 ttttgatccg gaatggcgct tcattcccat ggtgggggcc tcaggttcca tttacggcat
    14761 catggccgca tgcgcggtgc tctttcccca tgcccgggta cagcttctgt ttcctccggt
    14821 taatttgagc gtgcgccagt ttgccctggc cgttctcgga gtcgccgcag ccgtcattat
    14881 cttccaatgg aacaatgcgg gcggggaagc cggccatctg ggcggcatgt tcgccggatt
    14941 tattctgaca ttgctgattt tatggaagga aaagatgtct tccccaagac ggaaagccgc
    15001 ctcttcttcc cactattccc ctgagccttc cagccgccgc ccggcacagg cttacccctc
    15061 tgaaaaggag gtggatgaaa tcatggaaaa aatatccagg tccggcctgt ccagcctgac
    15121 ggaggaggaa cgggaaattc tccggcgcgc ttcccggcgc tgaactattt gttagatatt
    15181 tttatatttt tcgttatttt ccttcctttt tgccatccat ttcctagcat ggatgaatga
    15241 tgacgcgact gtattcctcc accgtcctgg gcgtagatgg cgtagaagtg gaggtggaag
    15301 tggatttccg ccccatggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15361 nnnnnnnnnn nnnnncttta ttgtcggcct tccggatgcg gccgtgaaag aaagcgggca
    15421 gcgcgtggac acggccatac aaaacagcgg ccttcccttt cagcagggta tcttcgtcgt
    15481 taatctggct ccggcggatt taaggaagca gggccccgga ttcgaccttc ccatcgccct
    15541 gggaatggtt gcgggagcgg ctgaaaagga gatggacgct tctgcctggt gcattgtggg
    15601 agaactggcc ctggacggaa ccgtacgccc cgtgcaaggc attctgccgc aaatcatgga
    15661 agcgcggcgc atgggcagga agcgcatcat gctgccgcaa gccaatgcct atgaagggac
    15721 gcccgtacag ggtgtggaaa tttatcccgt ttcttccctg agagaagcct ggcatttgct
    15781 tacttccgac gccctgccgc ctccatttac aggttcaggg cgagaggccc atgctccacg
    15841 ggacaaagac gccgctgtgg attttgacga aatcaaaggg cagccttatg cacgccgcgc
    15901 catggagatt gcagcggcag gaggccataa tatcctgctt tgcggctctc cggggtccgg
    15961 caaatccatg ctggcgcaaa ggctcccgac cattcttccg cctctgagtc cggaggaagc
    16021 gctggagacc agtaaaatcc actccgtatg cggtctgctg aaacggggga acggcctggt
    16081 ggaccagcgt ccgttccggg ctccccacca caccatttcc gatgccgggc tgatgggggg
    16141 aggtgcgaat atcacgccgg gagaagtatc ccttgctcac aacggcgtcc tctttctgga
    16201 tgaattgccg gaattccgcc gtgccgctct ggaaaccctg cggcagccgc tggaaaccgg
    16261 ccaggtagtt atttcccgcg cttccggcac catgacattt ccgtgccgtt tcatgttggc
    16321 agccgccatg aatccgtgtc cctgcggtta tctgggagac agaagaagag cctgcacctg
    16381 ccccccggcg cagatcgcac gctaccgccg taagatttca ggcccgctgt tggaccggtt
    16441 tgacctgctg atggaagttc ccgccgtgga tccctccatt ctggcttccg cgcctgccgg
    16501 agaatgttcc gccagtatcc gggaacgcgt ggcggccgca cggcggctcc agtccagcag
    16561 atacgcagga acgcctttcc gcaataatgc ggccctttcc ggaaaagcgc tccagaagta
    16621 ttgccgtctg ctgccggaag ggagggctat cctgctccgc gccgtggaag aactggccct
    16681 ttccgccaga gcctatgacc gcattttaaa ggtggcccga accatagcgg atttggaagg
    16741 aacttctgat attcaggatg tccatttgta tgaagccgtg cagtaccgcg ctttcgagca
    16801 atcactgagg gattagcact atgaatctct ctcctttcgc aactacccca aaaacaaaga
    16861 catattcccg ttccgaaaat aaaaaagagg cagcttttca gctgcctccc gaataacgag
    16921 gatatttact atgaacgtct gcggcgcaac agcatagctc ccaggcccag caggcccaaa
    16981 gacgctgcgg caggctcggg aaccgtttcg taggaaacct gtatacctgt aattgtataa
    17041 gcggaagtag cgcctccagc accaccgcgc actccggcaa ctaccttata cccttcctcc
    17101 caggtaaggg gagccgccga catatccagg gaaattgtcg ttgtttccga tgtagaggta
    17161 aatgaaaagt cctgtatctt ggaaagttcc tcaaccagag tgccggttga atcatatacg
    17221 gcaagcacca tggaataact gctgattaca gaagaaggag tattacagga aaatgtaatg
    17281 gaagaaacgg aatccccctc ctgcggccct ccaagaacca tggcaaagga agaccccgct
    17341 actccggaac gaccgcataa tgtaatggag cctacatcat cctggttaac ccaggtgcct
    17401 tcaccgttta ccgaccaatt cccaacactg ttgttattgc cgccggcata tattccattt
    17461 tgcaggccct ttactttgtc cagaagctct tgccccgatg ttctcgccat agtggcggtc
    17521 tgtgatacgc tttgcattga ttagaggatt gttaatccgt tgtcaacata aaaagttgaa
    17581 agagcggtat tccggcagtg gacttctttt ttgatgtaag gactttataa agactaatcc
    17641 ttgcgcctct tccattctaa catctgcatg ttatttcata aatcattcat tattatgaat
    17701 cgaacgttat cgtaaaccat tcatgaagaa aataggtcca gcgttttccc catgatggta
    17761 cggaaagaaa aagagcggat taacaatccg ctgtcccttt tattcaatcc tgacgctaaa
    17821 ggaaaagccg cctcagcaac agtgaggcgg ctctctccaa aaagaatttt taacgaatag
    17881 cttccaaagc ctgtttgaga tccgcaataa tgtcttccgc agcttccgtc cctacggaaa
    17941 accggatgag gtcaggccgt acgcccgcct ccatgagctg ctcatccgtc aactggcggt
    18001 gcgtatggct ggcgggatgg agaacgcagg tacgggaatc cgccacatgg gtcacgatgg
    18061 cggccagctt cagactatcc atgaacttga tggaacgttc acggcctccc ttgatgccga
    18121 aggttactac gccgcaggac tggccacagc ggaattgctt ctgcgccagt tcataatact
    18181 cattggaaga aagacccgga taattaatcc aggcaacatc ttcctgccct tgcaggaatt
    18241 ccgccacttt ctgcgcattt tcacaatgcc gcggcacacg caggtggagg gtttccaaac
    18301 ccaagttgag caaaaaggca ttctgcggag actggatgga acccaggtca cgcatgagct
    18361 ggacagtcgc cttggtgatg taggcgccct tgccgaagct tttgctgtag gagagcccgt
    18421 gataggaggg gtccggttcc gtcaaacccg ggaatttatc cttgtgcgct tcccagtcaa
    18481 agttgccgct gtccacaatc acgccgccca cggcggcggc atggccgtcc atgtacttgg
    18541 tggtggagtg ggtgacgata tccgcacccc attcaaaagg acggcaatta atgggtgtgg
    18601 caaaggtatt gtccacaatg aggggaacgc cgtgcgcatg ggcaatgtcc gccattttac
    18661 ggatatccag cacgcaaagc gtgggattgg aaatagtctc cgcaaagaaa gctttcgtat
    18721 tggggcggaa cgctttggct atttcctccg ccggggcgtc ctggtccacg aaagtaactt
    18781 ctatgcccat tttccggaag gtgacggcaa agaggttgta tgtgcctccg taaatcgccg
    18841 aagcactgac gatatgatcc ccagcctcgc aaatattgaa aatggcaaag aaggaagccg
    18901 cctggccgga agacgtcaga atggcggcca caccgccctc cagttcggca atttttctgg
    18961 caaccgcctc attggtgggg ttctggaggc gtgtataaaa gaatccggct tcttccagat
    19021 caaacaattt tgccatttgt tcgctggtct cgtacttgaa cgttgtgctc tggtaaatgg
    19081 gcaatacgcg gggttcgccc tttttaggcg cccagcccgc ctgcacgcac agggtttccg
    19141 gtctcaattc ctgattcatc atgcggggca tgctatgcga tgccgccagc cttgtaaagc
    19201 cgggaaaacc agacgtatgg ccgaagcacg ctcccgggtt tttcccagtg taaaactccc
    19261 tgttcccggc aaatcaaccg caaggacagg agcgttttac ctgcgcgcgg gataccctac
    19321 ggtcgtatga aacggcaatt cctccgatac ccctttcccg gaaccaacct tttcccggcg
    19381 aataaagccg ggcatctcac tacttgattt ccggcaaagg gatggaaatt tcctcttccg
    19441 tcagataaca gccgggatcg gacccgtacc agtgtccgtt ggcaaacgcg gcacgggtgc
    19501 ggaagcctcc gccgcacagg gcgaagtgaa cgcagcggcc gcaacggcct tcaatcttct
    19561 tctgccgttc cagaggatcg tcggaaccgc gcagctcagg caacatctcc gctgcagctc
    19621 cggccctggc atcccataaa tcggaaaacc ggtcactttt cacattgccc agagtaacgg
    19681 attgccagaa ctggtccgga tgcacggcac cctgtgtgtc aatgttggcg atgccccggc
    19741 cggaactgtt cgcgccaccg ccattccact ggagcaggcc aagggtttcc ttcgccagag
    19801 aggaaccttc ccggagctgg cgcagaagca ggtaaatgcc gtccgcaggc tgagtcacag
    19861 tcagcacctc acggtttttc ccctctgctt tccattcctc cacacgggca atcagcgtat
    19921 ccatggcatt ccgcgcttct tcctgagtca gggtctgcac ttccacgccg cggccggtgg
    19981 gaaccagatg gtaaaaacaa acgcgctgga tgtcgttggc gtcaataaaa tccaaaatct
    20041 ggcccatgca ctgcacattg ttgcgggtca gcgtcaggcg caggcctgtc ttttgcccca
    20101 cttcactgca gagcctgaac ccgcgcaccg cctgctcaaa agaaccttcc acaccgcgga
    20161 atttgtcatg aacggcgcca atgccgtcca gggaaatgcc tacgtacgcc acgcccagtt
    20221 ccttgaatcg cgctgcggct tccggagtga tgcgggtgcc gttggtggaa atggtcacct
    20281 tcaacccttt cccggtggcg cgttccagca gttccataaa gcgcggatgc accagcggct
    20341 cccctccgga aaaaaggagg gcgttcacct tgtagtccgc caaatcgtca atgaccgcgc
    20401 agcactggtc ccaatccagc tctccctcaa atttccgggc agaagcatcc gcataacagt
    20461 gtatgcattt gagattgcag gtacgcgtaa tgttccagac cacaatgggc ttgcgtacgc
    20521 gggacgaagc cggcgcgcag gattccgcag cgccccctgc aaaacggccg tgcccgtggc
    20581 cctgtccgta tctcaaatgg tccgcgggct gttccgcccc tgtccaaagt ctggtaatat
    20641 taatcatggt gttgtgaaag cctaggctga taagggcaga gattgtcttc cgccatcatg
    20701 tctccgtaaa cggcataggc gcgggaccgg gaccctccgc agatggcacg gtactcacac
    20761 cgtccgcatt tgccccgcaa cgcattgtca tcccggagcc tgaggaaaag agggtgcgtg
    20821 cggtagattt ctccagggtc atccgtccga acgttccctg ccgtcatggg caaaaaaccg
    20881 gagggctgga tttccccggt atgggaaata aacataacgc ctttgccgtc ccgggtgggg
    20941 atggcgcggc gggcaggctt gctgccctgg gcgcgggctt cctgaagcgc cacgcggcgg
    21001 taatggtgcc cttccgtagt tttaacgcca aagggcacct catggcttat cctgctcagt
    21061 tttttccaga cggcttccac ttcttccgcc gcaggcattt catccatggc ggcacggcct
    21121 gtgggcacca gaagaaacac gctccacatg tccggtttca ttttcttcat cagctccaca
    21181 aagtcgtcaa attcgcccag cgtactcctg gtgatggtgg tgttgatttg caagtgcatg
    21241 ccggcttcct tcgccatttc cgcagcccgg agagtccgtt catatgtgtg gggaacgccg
    21301 cggaaagcgt catgggtact ctcatgcgcg ccgtccaggc tcaacgacat gctctggact
    21361 ccagcttctt tcaacgcacg gaaatccgtg tgcagcagcc gggcggtagc agccgggctc
    21421 aatgccacat gcagcccctt gccggaagcc gcacggatca attccagaat atccggcctc
    21481 atgaccgggt cgccgcctgt cagcaccagc atgggaggcc gcagttccgc caactggtca
    21541 atcaggcgca atgcttcctc atgagtcaat tcatccgggt ggggacgcgg ctgggcgacg
    21601 gcgcggcaat gtctgcacgc aagcgcgcaa gcgcgcgtca cttcccagaa gaccaggaaa
    21661 gggcgttcat tgagatctgt ttcactaaaa accgggcgca tttttcagga cagccaattt
    21721 agaacaatta taattttttg accagaccaa aagggcgcat cctttaaaaa gttcacttag
    21781 ccgcctcttc acggattttt tgaacttcct taggcagttc ggaaagggaa ttcagagctt
    21841 ccttcaggtt cctggaagca tccggagaaa gcatgatggc cgtcacatgc ttgacaatgg
    21901 gctgcatcat ttccacaagc tgctgctgca ctcccatcag cttggcctgc tggttgcggg
    21961 tgagaggcgg cagggcggcc tgtttcctgg caacgtccag catagccgcc cggtagcgac
    22021 ctatctgttc cacagcagcc ttgccggttt cttcatccct taccctggcc agagagtcgt
    22081 taagcttttt tacttcctcc acagttctgg ttagcagggt ttcatgcgtg ttgccctccg
    22141 catcctccgg gaacggttcc gcctgctcgg ccttatcccg ggaagcctgc gccagctcct
    22201 tctggacaga ctgtaaatcc gccagggctt tttgcaaatc gggcgtaagc agaccttcct
    22261 ttttcaaacg ggacagagat tttattaatt cacctgaaat tttctgtgct tccgtcatct
    22321 gggcggaaac ctgagcctgc tcctgaagcg tggcaccagg aagctccagc tgtcttttcc
    22381 caatgtccag cagcttttta tgaacttttt taaccgtttc cacagcagcg gccgccccag
    22441 gttcatccgt taccttgtcc agagccgctg cgatttcatg catgctggca acgctctgcc
    22501 tggtcaggga aagatgggag tcctgggcaa atgcggaggc tgttatgcaa acggcagtgc
    22561 ccaggctgat ggaagcaaca attttattca tgatacggaa gcctttccgg tatggaagaa
    22621 tacgttttcc tcctttcccc gtcaatggcg gctattgtca agaccactat atgaaaaaaa
    22681 aggctgtccg cttgtttgca gacagccctt ttgaaatctt aacagcccgg ccttagtcgc
    22741 gctgctggcg tgaatacttg tagtagctct ggaagctgag ttccgcaggg aattcgctca
    22801 ggccgccatt gggattgtat tccttgaggc cgctcatgcc gcaacgcccc tgataagggg
    22861 gcttgtatgt gcatttgtaa gtgggagccc agaaggtgac aagttcataa attccgtagc
    22921 ccatgcgttc aaacatggta gcggtgccat caacaatgcc tacggaaaaa ccggcataat
    22981 ctccctcgcg gctcgtccag ttgatgattc ccagcgggat ttcttcaacg gaataaataa
    23041 ggttggcgat ggcgcggccc agcttgcggg tgggggtgta ttcggaagcg gggggagcct
    23101 gaatgtccgc actggcgaca ccaacgacgc cggcggcaaa aagagctgag atgatggcac
    23161 gtttcatact gattgtttat atagtataat ttgtcgaggg aaagcaactc aaaaaaacaa
    23221 aaaatcaatc taattccggc cgggggtccc gcgtcatgag ttccgcaagc gcttccatcg
    23281 gcggcttgga ttcataaaga acggaataaa cggcgtcaat caggggcgtc ctcacctcca
    23341 gcttccgcgc aatttcatag acggacagcg tgttgggcac cccttccgcc accatgccca
    23401 gggtatccag gatttcctgg aggctcttcc cttccgccag tctcctcccc accgtctggt
    23461 tgcgggagtg gcgggaatag caggtaacaa tcaaatcgcc tactccggaa aggcccatga
    23521 aggtttccct gcggcctccg ctggccacgc cgatgcgcgt catttccgcc aggccgcggg
    23581 tcagcaaagc ggcctgggcg ttatccccca ggttcagccc ttcgcataag ccggccccga
    23641 tggcaaagac gttcttaatg gttccgccca gctgcatgcc gataatgtcc gtagcggaat
    23701 aaacacggaa ggttttggat gcaaatatct gctgcaccca atccgcatac ttcggatctt
    23761 caaatccaat cagggaggcg gacggaaggc ccaggcagat atcttccgca tgattgggac
    23821 cggaaagcac gcctataggg ttggagggca gatattcctg aaggatttcc gtcattcttt
    23881 tatgggttcc ctgttcaatg cccttggtgc aggagacgat gacggcatcc ggctgcaccg
    23941 gcaggctgca cagccgctgg gctacggaac gcatggctac ggaaggcacc actactacaa
    24001 tgagttccgc atccttcacc tggcccagat cgcaagtagg atggatattg ggggccaggg
    24061 gaagggcggt tttcgaaagg acggggctgc gtcttgtttc ggcaagctgc ctggcctgag
    24121 cctcttccgg cgtccacagg acgacttcac attcccgggt cgccagaacc atggagaggg
    24181 ccgtgcccca ggaaccggct ccaatgactg ctattttatg taaccgattc atcatcttac
    24241 ttggactttt tggaatagaa ggagtgctcc gttccgttga gaagccttgc gatattggaa
    24301 cggtgcttga gaatcaccag tgcgcctacc accgtgaaaa aggcgacata aagtatctgg
    24361 ggggagacat ccccgtcctg gcaaagcgga taaatccacc atccagcggc gatcatcacc
    24421 acagcggcca ccaggctggc cagggacacg atgccggaga ttcccataac caccagccaa
    24481 gaagccagag ctacgcaagc caccaccgga ttgagggcaa ataaaacccc cgcggttgtc
    24541 gctacgcctt ttcctcccct gaatcccagg aaacaggtgt aagtatgacc gagtataaca
    24601 gcgaaacagg agagaactat caagacattg aaatcccagc cgcccgcatc ccccgtcagg
    24661 aacggcagcc agttcaaggc gccgaacaca gggatgaacc cctttaagaa gtccagaatg
    24721 aaaacggaaa ggccccactt ccagcccagt acgcgccacg cattggtagc gcctatgttg
    24781 cgggaaccct gctgcctcag gtcaatccct ctgattctgc cggcaaggta gccgaacgga
    24841 atcgctccga tcaggtaata gaaaactatc caggcaatca tgttgtgcgg agtgcatttt
    24901 aatcccccac cggtcaaatg acaatgggga aatcacctcc ggcccggttc tttccggagg
    24961 ggttgctccc gtaaaagaac accatacctg acagcctcct ccagatcctc attttcaagg
    25021 ctctccgtgc cccggtctgc ggaaaagccc tgtttaaggc gcaataagaa cagcgcgaac
    25081 attttcccgc ccgcccagaa ccatgctccc gcacttcctt cgccggaata agcttattta
    25141 ccgccctcgc agggtttccg catgacggca gcccagacgg aggcattatc ctgctggggc
    25201 tccagcatat ccttcacctg ttccataata atccccacca gctccctgct ggaacaatcc
    25261 ctggcctttt gaagagccag gaaagaaggt tctctcaaca gctcgctcca aacctgcaag
    25321 ccatccgagc atgccagcac cgcatctccc ggatgcagga gcaggggcat ggcgctgatg
    25381 tccaccagtt ccatgggacc gcccataacg gcggaacgga gaatgcaacg ctgccggagc
    25441 gcaccctgaa gtgacatttt ccccttccgg taaaggcaat ccgcaatcgg tctcatggaa
    25501 tggtcttcat tcagacggtc catgcgaaag ctgtccgctc cgctccataa atacaggggg
    25561 gagtctccca cgctgatcca ccacaggaaa tggtcgcgga tgaagacggc cagcaatgtg
    25621 gttccggatt cctccggttc cttctgaatg gcggccaacg ccctgttggc agcatgcaaa
    25681 gccgccgtta aacgttccgg gatatcctcg atgtcgcttt ccgcaaatac cgcagcaaat
    25741 tcctgcgcca ccacacagga ggcgcaatct ccgcgaagat gccctcccat gccgtcgcac
    25801 acgatgccca gcatcccgcc ggcggcggga agacttttaa ccacgtcttc ctgctcgcgg
    25861 cgtttgccga tccattgggc ggaagcgcag tccagaatat ccggggtggg gggggccatg
    25921 cggccggtgg ttcggattaa ctgtccttac gtttcaactg cgggaagagc agcacgtcac
    25981 ggatggaaga ggcccccgtc agcatcatga cgacgcgatc gatgccgatg ccgatgccgc
    26041 ctgcggaggg cattccgtat tccagcgttt caatgaaatc atagtccaca cgctgggtct
    26101 ccccggcagc ctggtgctcc aggcgttcct tttgcacgtc aggatcgttc aattccgaat
    26161 agccggggga aatttcctgc ccgttgatca ccagttcata tacgtccact acgtccggat
    26221 tttcccggtt cagcttggcc aggggaacca ggtccttggc cacgtgggta acaaagcagg
    26281 ggttcatggt cttttcctcc accagttttt catacacctg ctgggaaaca tccacatctt
    26341 tcatatccgg gctgatttcc actcccagct cttcacagcg ggcgcggcgc gcttcggggg
    26401 agatgtcaaa ccagtcttcc cccgccacgc cccggatcag gtcctggtag tcggcgcgtt
    26461 tccagggccg gctgaggtcg attgtgtaaa gaacgttgcc ttccgcgtcc ttgtggtcaa
    26521 tctgaaggcc accgcagaat ttttccgcca gatggcagat gagctcctcc accatattcg
    26581 ccatggtttc aaaatcaccg caggcgcaat aggcttccag catggtgaat tccgggttgt
    26641 ggcggcggtc aattccttca ttgcggaagc tgcggttgag ttcgaaaatt ttcgtgaagc
    26701 cgcccaccat caggcgtttg aggaaaagct ccggggcaat gcgcagcgtc aacggcatac
    26761 ccagcgcgtt atggtacgtt tcaaacggct tggcggcggc cccaccggcc acatcctgaa
    26821 gcatgggggt ttccacttcc agataaccgc gttcctggag aaagcggcgg atttccgcaa
    26881 tcatgaggga gcgcgtaacg aacagggagg cgctttcctc attggaaatg aggtccaggt
    26941 ggcgcttgcg gtaggtcacc t
//
LOCUS       DAOMSU010000005        24999 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_52_length_24999_cov_2.850906, whole genome shotgun sequence.
ACCESSION   DAOMSU010000005 DAOMSU010000000
VERSION     DAOMSU010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 24999)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24999)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24999
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52_length_24999_cov_2.850906"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            437..1579
                     /locus_tag="PK077_00535"
     CDS             437..1579
                     /locus_tag="PK077_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRN22818.1"
                     /translation="MYKHYSDGRRRFLKMSALFGTAALISPAIARAEGGGADSPENGG
                     FAASRSRTLGKGSCALEASPLGFGVMGMTYNRSQSPSREQCIRLLHEAVERGVTLFDT
                     AIIYGPLSNELLAGEALSPFRGKISVTTKFGHEVINGKGTGRQDSRPETIRRYCDESL
                     RRLKVDAIELFYQHRFDPRVPVEDVAGTISELVKEGKVRRWGMCEVTPGTIRKAHAVH
                     PLTAIQSEYHLMHRDVENNGVLDVCRELGIGFVPYSPLNRGFLGGCINEYTQFDPNND
                     NRQTLPRFTLEAMRANMRIVNILQQFGRTRGMTSSQVALGWLLQKAPYIVPIPGTTKL
                     SHLEENLHTLDFTCSPQEWAELENAVAAIPVTGARYNAEQQRQVGH"
     gene            complement(1807..2271)
                     /locus_tag="PK077_00540"
     CDS             complement(1807..2271)
                     /locus_tag="PK077_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HRN22819.1"
                     /translation="MQDASELGKQKDMCLSFSEVSELTGTTPSNLRFFEEEGILDIPR
                     NGAGCRVFHRKEMEELLGLVCLKHSGLELKEIKRFFHLVRQGNSTLRERYRMLKEGHQ
                     AMKTRIRELKKCLKYSKIKMEYIGQCCEAYDKGEPLPQVNPEWMDSFLKQKI"
     gene            2506..3543
                     /locus_tag="PK077_00545"
     CDS             2506..3543
                     /locus_tag="PK077_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRN22820.1"
                     /translation="MILFNCDYSEGAHADILRRMAETNMEQTTGYGEDPYCDQARKLI
                     AGLCGRTDLDIHFLIGGTQTNFTVIAAALRPHQCVLCADTGHINVHESGAVEACGHKV
                     SAIPSPDGKLTARQIEEAWHTHWDDETREHMPQPRMVYISQPTELGTIYSRKELQEIS
                     GVCRRRGLYLYMDGARLGYGLCDEDNDLDLPAIASLCDAFYIGGTKVGALFGEALVLR
                     HEALKEDFRYIAKQKGGRLAKGRLLGIQFLELFRDGLYFRLGAHADRLAVMLRDFCRS
                     RGLALPFSSGTNQQFVTMPDSVLEELGRKYGFAYLRREDETHSTVRFCMSWATREEDV
                     RELMADIARLC"
     gene            3652..4968
                     /locus_tag="PK077_00550"
     CDS             3652..4968
                     /locus_tag="PK077_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22821.1"
                     /translation="MVMPLIVGQEWRRRISRMQWLLNAVLAVECLSMPFFAAGAVAGM
                     VWYGGRRMGADWGWLTGGALAAAGLFLAVWGWLRLRGRFFSRRDTAAFLDEQLGLHAA
                     LSAGEEWGNAVEAGESAVQGNSVLRIRSAWCLAWLAGGAVLMACGAFLPLPAPQAVGQ
                     LPDLPPVLARVGEALDRLAEMESVDNKSLEPFREQLEDLKRMHRNEMYSHAGLEAADA
                     LKGKTAAAVAGLSNQMYRTDSALSLLDSLNGASSPEAMQSLQEALTGMESLALQPGGI
                     MSRQLQELEASLPSRQIDPETALRLREQLERAAQQLRSMCGQCGISLMASPDDRTVME
                     AGQGEGSEPGRGGTGRGRGDAPLAFEMEERPRLDTVSRRVVHEDLSRAALGEAVGVES
                     AAPTPEGAGNGLEHGGGGDRPARGGDAVWSDCLTPQEQSALKEIFR"
     gene            complement(4961..7540)
                     /locus_tag="PK077_00555"
     CDS             complement(4961..7540)
                     /locus_tag="PK077_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRN22822.1"
                     /translation="MIQFNTPEWFFLIPLLLAAGWKYRRLRLASPLRLLLQACLLLAL
                     AQPALDRGGKGMDLWVLVDQSNSTAGIPAAGAEEIQAILERHKHAGDRVRLVDFAQSA
                     VLRGRGDPVFAGGTAGTDMEQALAYTMALMSPNRTNRILMLTDGWPTTPLDQAAEQLI
                     QSRIPVDYRLSVTFREADIRIEQIRTPARIRPGEAFILEATLAGPPGPAITVPWQISK
                     NGGAPLRGTATLLNGKATVRLADRLSTPGCSAYEMTITPQNDPIPENNRAVSFLEVTG
                     GNGVLLLSGYDRDPLAPFLEAQGFRVQQPSGLNRLDARHLSGVGLVIINNLSASSVPP
                     DFLHALDYYVREQGGGLLMCGGRHSFGSGGYFSSPIDELLPVSMEMKKDKMKLMTAMS
                     IVLDRSGSMSCSVPGGKTKMDLANAGTCQTISLLSDQDLISVHAVDSEPHPIVTLSSL
                     GPNRKKMISSVSRISSMGGGIFIGAGLKAGWQELQRSVAGTRHLLLFADADDSEEPAD
                     YRETLKEMVKEGATVSVIALGTEKSADAGLLREIAELGRGRIFFCDRPGDIPSIFAQE
                     TVSVARAAFIRERTLLRGTAGWLQIAAGQPEWPPAVDGYNLCYLRNGATAACVTEDGN
                     AAPLVSFWNRGTGRTAAVTFAMGGELGKNIQQWDGYGDLIQTLARWLNRKNPPQGYSV
                     RADTAGDRLRIRLYYSEENIPRLAERMPEISLELSGKESSRTQNGIWEHLQPGIFQCS
                     FPLPHGVMARGAVRIGGSVIPFGPVSQQVDPEWAMPPESGNAFLDLVNRTGGRERMDL
                     PSIFREPRPGTSLQLRPILLWSACVLLVLDALFTRTGLLPRGQPRQRVTRSRPSRENN
                     QSI"
     gene            complement(7537..9264)
                     /locus_tag="PK077_00560"
     CDS             complement(7537..9264)
                     /locus_tag="PK077_00560"
                     /inference="COORDINATES: protein motif:HMM:NF019220.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HRN22823.1"
                     /translation="MIPHFTNTTAFWALLAIPLITAIHFLQHKTKTQATATLFLLEAL
                     VPEAHEGNAWDRLRASRSFWMQILAVLLLTWVLAAPAWPGKDSGQTVVFILDDSANMT
                     PFRQEAVNAVSEDMDAILRSGIPATWVLMGSRASGQPLYRGPSARQALHALDLWQPRE
                     GTHDLAPVLRTATAVAGPTGITRLITSTARRVPAGQSARGVGKLLENVGFAGITPVET
                     VGPDHWRIAVKNNSPSPLRSEISIHTEGGRPPARRSLFLNPGTVTEFEYSLPPECGKA
                     VLRLPTDAFPADNELLLVRSAPAPVAVSMGIPEQSGKIFLNIIHSLPGFSPVPDGSDP
                     DLLLLEGKTENAKAPGKAAIIFAASGKPSYGAVTVERHPLTDGLNWSGLLIPSIGSMK
                     PGEKAGVLLWQGESPLAWVDGKRLFLNWPWEKSNADRVPAPLLMTRRFMQSVQENLPG
                     THYGNLPGGTLLSMPAGGKLIQTMPGGERRETVFNGRLPEETGYVEIFPPGEGKTPLF
                     QGSVWFSDARMGDFSHCSTFDTGLPQPHEEALRHMKRDPLAPLWLALAFLALILSWLP
                     PVPDISLRP"
     gene            complement(9300..10169)
                     /locus_tag="PK077_00565"
     CDS             complement(9300..10169)
                     /locus_tag="PK077_00565"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRN22824.1"
                     /translation="MTSFPFPSQVIQPDTEQAARRLRLPFSCCAWKGTQGHWEGTGAG
                     NSIDFQGSRSYQWGDDPRDIHWAAYARTGQLTMKVFRAELSPQVDVAVDVSESMFFHE
                     ERAARTRGLLQFCLLSAIGTGAQVKIHAVKGSRIIPLDQEDVLSGQWEAQLQSLPPDE
                     SMPSISIWRPNGMKIFISDLLYPGEPDNLLETMASLKGMSVILAPTLQEEAELPAGNA
                     RLKNCESGHLRRQLITPSLSRRYARAYAAHFELWISACLCRQTVLARVPCKGTLTEAL
                     SGEALRQGAVELS"
     gene            complement(10204..11652)
                     /locus_tag="PK077_00570"
     CDS             complement(10204..11652)
                     /locus_tag="PK077_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22825.1"
                     /translation="MNTSRSLDFPIWMPSALVKELRQGLRTPSFILLVSIVPSLLSFF
                     FLFSFIINPLDGEPFISPDGCQIIFWVTMVVALLFIMPLRASTSIRNELLTRNNELLL
                     LTRQSAGRIVLGKWISFMAQSLLIAFISLPFFLIRYYYGEINLVQDVTLFFLLYLGSG
                     MLTAYTLWASAMPSLMRIIAFSLMGIGILTLGGSHENLDFFRDGAGEGVLLIFLVLAD
                     AFLVISSLLLLAREWFSPTAQNTAAPLRKLLLAFFAASILPLPFLENASGSIQNIMTG
                     NSIFVMVYGISLMIINLNTPARLLPIHMEQMKNKRFSRLQQLLFLPGMPGSVLFTLLL
                     CALAAIAVYLIDRMASIPEFQSPQRLFCLAAGGWYAITMPAMLLKPFWEKLKNNTLLT
                     YTMICLILCLVQNVLWGVNLSVPLLPGGYLSELLRQETLPAFSKFSLFSLMDLAAGIA
                     VLALTSKHWIDIFRNASPEPLQKPGNSRRERE"
     gene            complement(11649..12656)
                     /locus_tag="PK077_00575"
     CDS             complement(11649..12656)
                     /locus_tag="PK077_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN22826.1"
                     /translation="MSTPLLLEVSGLSKSFGPLKAVNNASFVIRQGQVVGLIGSNGAG
                     KTTLMRMLATLEMPDSGHIRLNGADVVNHPELARSRIGWMPDYFHPYKNTSVREYLDF
                     FARACGMGRERLLDAVDEVMDFTELAGLQNQMIDKLSKGQTQRLCLARTLISDAKFLI
                     LDEPAAGLDPKARLEFKNLVHLLKARGKTLLISSHILSELGEMCDALIFMNAGTVIHD
                     GNMEDLLHRQTESGYAFEIRTAGDNTASLEEWLALRRGWNVRHVQQQKVVAAFVSCEP
                     EAVAAELRQLCLDLPVIDFHRSERRLEEAFVDILIHGSGEPAPRQNPASSQESPSIPA
                     S"
     gene            complement(12659..14242)
                     /locus_tag="PK077_00580"
     CDS             complement(12659..14242)
                     /locus_tag="PK077_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22827.1"
                     /translation="MRRPLSRSWLFFSLFLLSAFNILPLHAKPDAAPPIAVSVEKAFD
                     YSANGARVPLRVKLENTTGSDRKATLQFHEGWNGNRRTSHTYPFFIPARESVHAVVYP
                     LLSYNLVYEVKENHEGASPIPTDTEHRKIVAVIQEGAPADWHLLSESSYNAAISSCDL
                     MKWPADYRMYEGQTCLIIPEKTYRTHFDEAHRKAIRQWVTGGGNLWLIGDRGLSVSTR
                     LLGNGRILHVPSLDGLPEEEKKLKLKEFINLHEKGNCYPEITSAAPYYFSAPSTTLGL
                     GLIIFAVLVGPLSLFLWAPAGKRQRLFLLIPAISVGFSLLLLLLILAGDGTGGTGSRE
                     VLIQVNPQDHSALISQNQICKTSVLLNNTFQLPENAGITGARMSKARGSIKEKPIEGA
                     ARQGEECGGKWFTSRSTLQHRIIMPVSTRAALTLLETSPGGAPVFQSTFPGTLNGLTY
                     RDASGKYWTLEQLPPGRKMQASPFLPEEEPDGPPPLHFRASMEPAGGELGPIPTLLSI
                     NWEKTSVTVSGPVTPQPHE"
     gene            complement(14267..15292)
                     /locus_tag="PK077_00585"
     CDS             complement(14267..15292)
                     /locus_tag="PK077_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRN22828.1"
                     /translation="MNTPPILSQEELGCAMQKLRALAAAMNQVLFGQKDLIELVLIGV
                     LARGHILLEGLPGLGKTELVKGLSRALDITARRVQFTPDLLPGDITGTPVLQERDGTK
                     NFIFQEGPVFANIMLADEINRASPKTQSALLEAMQERRVTVMGETHALPSPFFVLATQ
                     NPIELEGTFPLPEAQLDRFLFKINVNRTPADVLTRIVLNREMGEEPEISPVLNAQELQ
                     ELMQMARNVAIPEVVADYIGRLVNATHPGESEASGGVKYGASPRAALGLAAAAKARAL
                     RHGRAFCSFEDVQAVIHPVLEHRILLNHTARLDGQTPTAVINRLVREIPAQNKPLPDS
                     LSAAKIH"
     gene            15526..16710
                     /locus_tag="PK077_00590"
     CDS             15526..16710
                     /locus_tag="PK077_00590"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HRN22829.1"
                     /translation="MPSVYPPGNERIYVVDALRGFAVMAIMLLHFLEHFIYNSYPVAS
                     SPMMEAANQQFKEVFFFLFAGKSYTIFALLFGFTFAVQYRNQARKGRDFAGRFVWRMC
                     LLAVFACMNAAFFPGGDVLLTFSLAGLLLVPCRRLKTSSLVALSLFFLAQPLEWAYAA
                     VQWMHPGWVPPSLSVAESYASLKSAVDTGNFWVMAWENLTTGQWASLAWGIEAGRLMQ
                     APGLFLLGFVLGRQDFFLQNDGTAVFWIRGLLFSLAGACAFYVVMCLPGLPGPVHTVF
                     SMWHNVCFTGVWVAGFVLLYRMEYFRKATAPLLTYGRMSLTNYVSQSVIGSLIFFPYA
                     LGLAPYCGYLASFVVGFAAMTGQIWFCRWWLERHRYGVLEGLWHRATWLAAGKVSAPE
                     HR"
     gene            17084..18082
                     /locus_tag="PK077_00595"
     CDS             17084..18082
                     /locus_tag="PK077_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22830.1"
                     /translation="MAWCMERRTGIGGFFGLELPEYGNFPQWHPGRSVAVNSGRRALE
                     YILRRLGDVRCVRVPLYTCRTVVETMERLGIPVITYRIDERLEPEELPELEKGEYLLY
                     TNYFGIKEKCVDRLASRYGGSLIVDNALALYSPARSGVASLYSPRKFSGLPDGGIAVM
                     DAERSLPEPEERDESLPAAAFLLECLEHGQERASAACERNERRLRNTPLRRVSRLTER
                     LMSGIDYEQARLLRMNHFLFLHERLGHLNRLSVEADSLSAPCYYPFWTAFPELRNVLI
                     DKRVLIPLLWPEVLEQAPASSLERKLALHLLPLPVDQRCGPQDMERIARIVEAFYS"
     gene            18161..18844
                     /locus_tag="PK077_00600"
     CDS             18161..18844
                     /locus_tag="PK077_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22831.1"
                     /translation="MSDYLSSLGYPFRGRPMVFVFSVMMVWGMFLAARDVLIGWIAAF
                     CFLLPFMCAFFMKMVRQSAMGEEDICPFPELDEWLDSLMLPFVRLVVCIIVSFLPFAV
                     YVHTAAWAADTPLAWLFLAAGVIYFPGVFMRTSVRERFSGLSPVGWWGLVRRAPFSYA
                     GLLGAICAGGWIILNLPSGPLMDFAMVPVKLYVACVLMNMCGLFMLKHEFAGEEEDGS
                     FSPSGPAAS"
     gene            complement(18882..19934)
                     /locus_tag="PK077_00605"
     CDS             complement(18882..19934)
                     /locus_tag="PK077_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRN22832.1"
                     /translation="MNKSPKTVFSLLIPAVIFVAAGAAIFAFQNFSNKRVLSGFAGYV
                     YSKPIFGQNRFEGILIGPSSTGWAWRKEVSKVSITPDTTVEEFDARNGGAILGKDKLP
                     ISCKASLVYRLDPSRVKEFMEDYGGIAQSSGRNDDEVADEIMLFAYKNFIQQPFRTAV
                     RAELSQYNALDASGSLQKISDNVYTQLSKRLDGTPFIVDSVAVGETNPPQAIIESVVR
                     KVQTTQENERKETELQIARKDIAIQRARGEAEGAMKMEIARQEANANLARGEAEAKVI
                     VMRGKAQAEAALEAARAEAEGLALKEKAMGPNMLRAKAFENMLNNAKVYLPSGKDAEG
                     NMSVLGILNLDKEPAK"
     gene            20195..21064
                     /locus_tag="PK077_00610"
     CDS             20195..21064
                     /locus_tag="PK077_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HRN22833.1"
                     /translation="MNVAITGSGGFIGSHLSKRLEMFGHRVTPLRRYLFAQEGVDCLA
                     QALAGCHAVINLAGAPIDRRWTDAYKRELVESRIMTTRKLVEAVNRLHEPPGVMISAS
                     AVGYYGSSGGCHGELDAPEADSFLAELCVAWEKEAGLVNSSVRLVLTRFGVVLSPDGG
                     ALPEMMRPARFGVTATAGNPDHLFSWVALEDLADALVFIMGRQYISGPVNVTAPERTT
                     NREFYRAAAEHFHTRLSIRVPDAVLRLLMGEASQVITGGQCAVPETLMREGFTFQYPD
                     IRTFFNKAFSVKS"
     gene            21061..21570
                     /locus_tag="PK077_00615"
     CDS             21061..21570
                     /locus_tag="PK077_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRN22834.1"
                     /translation="MSMIQTIVTSLFHGHSVKTDPMPDFNLERYLGEWHEIARLENWF
                     ERGLSRVLARYDRREDGSISVVNSGYDVRTGERKEARARAVAGDAPNHLKVYFVPLVY
                     GRYEVAFLDENYTRAVVSGGSLNYLWLLARSPQLKDDELQPMLHCAEKLGYDTSRLIY
                     ANVQASPDK"
     gene            21908..22918
                     /gene="ilvC"
                     /locus_tag="PK077_00620"
     CDS             21908..22918
                     /gene="ilvC"
                     /locus_tag="PK077_00620"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HRN22835.1"
                     /translation="MDELLKEGVTMDIIHDNAADLSALNGKTVAVIGYGAQGRAQALC
                     MRDSGVNVIIGVRPGKSFDAAAQDGFQVMSVAEAAEKADIIHILLPDESHGAVYEAEI
                     KPHLKAGKTLCCSHGFAYVFNTIVPPADVDVIMVAPKGPGTEVRRVFEEGFGCPGLIA
                     VHQNPSGKARDVALAMAKAEGLTRGGVLECTMAQETYEDLFGEQNVLCGGLVDLMKYG
                     FETLTEAGYPPEMAYFECVHEAKLIVDLIYNGGIQKMNSVISNTAEFGEYYNGPQILP
                     AEVKERMKESLKRIESGKFAKDWLEEAAKGAPNLKAKREALGQHPVEIVGAKIRSLFE
                     RN"
     gene            23018..23815
                     /locus_tag="PK077_00625"
     CDS             23018..23815
                     /locus_tag="PK077_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN22836.1"
                     /translation="MEHATPAVNVENARVYLGGHEILHNISWQVQRGERCFILGANGA
                     GKTTLVKVLMGFAWPLFGARVQVLGKTFGHVNLLELRKSIAWVSPFMHKWLEDGSWNG
                     RDMVLSGPDGTIGLLREPTPEEEEKAAGIMKSLKAEHLIDRPVVAMSSGEQVKVLIAR
                     ALMTNPELMILDEPSVYLDLAGREFLLKTIEELAETRPDLTIVFITQRIEDILPVFNR
                     GMILKSGEIVAHGSRDEVLTEENLKDAFGLDIQLIKTEKGRLWTVIR"
     gene            23822..24517
                     /locus_tag="PK077_00630"
     CDS             23822..24517
                     /locus_tag="PK077_00630"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRN22837.1"
                     /translation="MKQSKTMELKKPVRVSLARQVLTAMESMIRSGKWKVGDRIPAEA
                     ELARAFSVSHNTIREALQSLIHMGMLEARPGDGTYVMASDRFAVAVSNRLKESDLPQI
                     LEARLALEKEIARLAAVKRTDEDLKELENALEDCHGRVRQGIEDDMLFHAAVARATHN
                     PVLSELYNVVIRHVQENLEKLLQEKQYDSGAMKLHDDLLAAIRKQEADEAENIIVKIV
                     EFDTVSIGGSCPS"
     gene            24677..>24999
                     /locus_tag="PK077_00635"
     CDS             24677..>24999
                     /locus_tag="PK077_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22838.1"
                     /translation="MKANVFSIMMLFFHLFPAYGIDPSVRGFEELHEVLKSAVRELDT
                     VQSPDHLPMAMEHFRALVEECGRNPDLVAVLELTSESCPPQLKKAYKAAMELQGKLHD
                     ASKRLA"
BASE COUNT         4932 a         6469 c         7917 g         5681 t
ORIGIN      
        1 aggatctgcg gaaaaaaatc agccgggagc tgggctttta ccacggaccg atgctccggg
       61 tgttccggga ggagttggat aaagccgggc cggatgatga atcctccgtc gggaaaagtg
      121 aagattccat tatttttgaa cgcaccaagc aaggtttgta aattgtccgc ttaccggcac
      181 tcagcaattt gttgtgcgag ggggaaagga aagcgtcccg ttccgagcgg cgctggtgca
      241 gaagaaggcg ggtatttgcc tccgttgcag gcggagtgtc agatgcgttc ctgatgcatc
      301 tttgttttcc gagccagctt tttgttccaa ggcgcttcct gagataaatt gctgtattcc
      361 cttgttgact tccagttggt ggaacccata tgctcggatg atatatcaaa atttcaacca
      421 tcaacgttta atgagcatgt ataaacacta ttctgacggc cgccgccgct ttctcaaaat
      481 gtccgccttg ttcggtacgg ctgcgctgat ttctccggcc attgcccggg cagagggcgg
      541 gggtgccgat tctccggaaa acggcggctt tgccgcctcc cggagccgga cgcttggtaa
      601 gggctcctgt gccttggaag cttctcccct tggcttcgga gtcatgggaa tgacctacaa
      661 ccgcagccag tctccctcgc gtgagcagtg catccggctt cttcatgagg ccgtggagcg
      721 cggagttact ctttttgata cggccatcat ctacggcccc ctgagcaatg aacttctggc
      781 tggggaagct ctttccccct tcagggggaa gatcagcgtc actaccaagt tcgggcacga
      841 agttattaac ggaaagggga cgggccgcca ggacagccgc ccggaaacca tccggcgcta
      901 ctgcgacgag tcgctgcgcc gtttgaaggt ggacgctatt gaattattct accagcaccg
      961 ctttgatccc agggttcccg ttgaagatgt agcgggaacc atctccgaac tggtcaagga
     1021 aggcaaggtg cggcgctggg gcatgtgcga agtcactccc ggtacaattc gcaaggccca
     1081 cgccgtccat cccctgacag ccattcagag tgaataccat ctcatgcacc gggatgttga
     1141 aaacaatggc gttctggatg tctgccgtga actgggtata ggctttgtcc cttacagccc
     1201 gctcaacagg ggattcctgg ggggctgcat caatgaatac acgcaatttg acccgaacaa
     1261 cgacaaccgc cagaccctgc cgcgtttcac gctggaggcc atgagagcca atatgcgtat
     1321 tgtcaatatt ctgcaacagt ttgggaggac gcgcggcatg acttcctccc aggttgccct
     1381 gggatggctg ctgcaaaagg ctccttatat cgtacccatt cccggcacca ctaaactgtc
     1441 ccatctggaa gaaaacctgc acacgcttga ttttacctgc tctccgcagg agtgggcgga
     1501 actggagaac gccgtagccg ccatacccgt gaccggagca cgctacaacg cggaacagca
     1561 aaggcaagta gggcattaac ccgatttgtt tgccgggaca atggcaatcg caacggcggc
     1621 ctccgcagaa cgttcgtcag gcgttccata ctatccggaa ttccttgttg attccgtttt
     1681 tacggattgt ctgaaaaaca tttccttctg aaagcattct gccggaagaa ggggatgtgt
     1741 tgttgcgtga agcgagtagc ctccatttgc ccctcccggc agcaggccca ggcgtcagca
     1801 ccttcttcaa attttttgtt tcaggaagga atccatccat tccgggttca cctgtggcag
     1861 aggttcgcct ttgtcatatg cctcgcagca ctggccgata tactccattt tgattttgga
     1921 gtatttcagg cattttttca gttcccgaat gcgcgttttc atggcctgat gcccttcctt
     1981 caacatgcgg taacgttcgc gcaatgtcga attaccttgg cgaaccaggt gaaaaaagcg
     2041 tttgatctcc ttgagttcca atccagaatg cttaaggcaa accagcccca gaagttcttc
     2101 catttccttg cggtggaaga cacggcatcc cgccccgttt ctcgggatgt ccaggattcc
     2161 ttcttcttca aaaaaacgca gattggacgg agtggtgcct gtcaattccg agacttccga
     2221 aaaggaaagg cacatgtctt tctgttttcc aagttctgat gcgtcttgca tgaagacact
     2281 atggcttttt cttaggctgc gggaaacgac aatttccgtt tatgggcggg gaagccggtt
     2341 attattgcct ttttaaggta catggcattc attttctcca actgcatgcg gccttccgtt
     2401 ttttctggag tttccagcag cgctgttccg gttccggtgt atttctcata ttcctttaca
     2461 tcaaaggttc cgggggatat ggtccccgcg tttttcagca aggcaatgat tcttttcaac
     2521 tgtgattaca gtgaaggcgc ccatgcggat attctccgca gaatggcgga aacgaatatg
     2581 gagcagacta ccggttacgg ggaggacccc tattgcgacc aggcacggaa gctgattgcc
     2641 ggcttgtgcg gcagaacgga tctggatatc catttcctta tcggcgggac ccagaccaat
     2701 ttcacggtaa ttgcggcggc gctacgcccc catcaatgcg tgctgtgcgc ggacacgggc
     2761 catatcaatg tgcacgagtc gggagccgtt gaagcttgcg gacacaaggt ttccgctatt
     2821 ccttccccgg acgggaagct gacggcccgg caaattgagg aggcgtggca cacccactgg
     2881 gatgacgaga cccgtgagca tatgcctcag ccccggatgg tttatatttc ccagccaacc
     2941 gaattgggca ccatttattc caggaaggaa cttcaggaaa tttccggagt ctgccgccgg
     3001 cgggggctgt atttgtatat ggacggagca cgcctcggct acggcttgtg cgatgaggac
     3061 aatgacctgg atttgccggc gattgcctcc ctttgcgatg ctttttacat cggcggcacg
     3121 aaggtaggcg ctctgtttgg tgaagccctg gttctgcggc atgaggccct gaaggaggat
     3181 ttccgttaca tcgccaagca gaagggcggc cgtcttgcga aagggcggct tctgggcatt
     3241 cagttcctgg agctgttccg cgacggcctt tatttccggc tgggcgccca tgcggaccgc
     3301 ctggctgtga tgctgcggga tttttgccgt tccagagggc tggcgcttcc cttttccagc
     3361 ggaacgaacc aacagtttgt cacgatgccc gattccgttc tggaggagct ggggaggaaa
     3421 tacggttttg cctatttgcg ccgggaggat gaaacccaca gcacggtgcg cttctgcatg
     3481 agctgggcca cccgagagga agacgtgcgg gaattgatgg cggatatcgc ccgcctgtgc
     3541 tgaaatttcc ctccgtccgg caatgccggg aaactgcgga aagggccgtt cccccattcg
     3601 tttgcggaaa agcatttttg tctttccggg aggaacctgt tgagccatga tatggttatg
     3661 cccttaatcg tgggacagga atggcggagg agaatttccc gcatgcagtg gctgctgaat
     3721 gccgtgctgg cggtggagtg ccttagcatg cctttttttg ccgccggggc ggtagctggg
     3781 atggtctggt acggcggacg caggatgggg gctgactggg gctggctgac gggaggcgcc
     3841 cttgccgccg caggtttatt tttggcggtg tgggggtggc tgcggctccg gggccgtttt
     3901 ttcagcagac gtgatacggc ggcctttttg gatgaacagc tggggttgca tgcggcgttg
     3961 agcgccggtg aggaatgggg caatgccgtg gaggcagggg aaagcgcggt acaggggaat
     4021 tccgtgctgc ggatacgttc cgcctggtgc ctggcctggc tggcgggagg cgcggttctt
     4081 atggcttgcg gggctttttt gccgttgccc gcgccgcaag ccgtggggca gcttccggat
     4141 ttgccgcccg ttctggcccg ggtgggggag gccctggacc gcctggcgga gatggagagc
     4201 gtggataata agagcctgga gcctttccgg gagcagttgg aggatttgaa aagaatgcac
     4261 cggaatgaga tgtattccca tgccggactg gaagcggcgg atgccctgaa ggggaaaacg
     4321 gcagcggccg ttgccggatt atccaatcag atgtatcgga cggattccgc cctttccctg
     4381 ctggacagcc tgaatggagc gtccagcccg gaggcgatgc agtcccttca ggaggcgttg
     4441 acgggaatgg agtccctcgc cctgcagccg ggcggcatca tgagccgcca gctccaggag
     4501 ctggaggctt ccctgccttc ccggcagatt gatccggaga cggccctccg tctgcgggaa
     4561 cagctggaac gggccgccca gcagctgcgg agcatgtgcg ggcagtgcgg catatccctg
     4621 atggcgtctc cggatgaccg taccgtgatg gaagccgggc agggagaggg aagtgaaccc
     4681 ggacgaggag gaacggggcg tggacgcggg gacgctcccc ttgcctttga gatggaagag
     4741 cgtccccggc ttgacacggt ttccagacgg gtggttcatg aggacttgag ccgcgccgct
     4801 ctgggggaag ctgtgggggt ggagtcggcg gctcccaccc cggaaggggc aggaaatggg
     4861 ctggaacacg gcggcggggg ggatcgtccg gcccgcggtg gagatgccgt gtggtctgat
     4921 tgccttactc ctcaggaaca gtcagccctg aaggaaattt tcagatagat tgattgtttt
     4981 ctcttgaagg ccgggagcgc gtaaccctct gccgcggttg tccccgggga agcaggccgg
     5041 tcctggtaaa cagagcgtcc agaacaagga ggacgcaggc gctccacagc aggatgggac
     5101 ggagttggag gcttgttccg ggcctcggtt cccggaaaat ggagggaagg tccatccttt
     5161 ccctgcctcc ggtccggttc accaggtcca ggaaggcgtt gccggattca ggcggcatgg
     5221 cccattccgg gtccacctgc tggctgacag ggccgaaggg gataacgctg ccgccgatgc
     5281 ggaccgcgcc ccgcgccatg actccgtgcg ggaggggaaa gctgcactgg aatattcccg
     5341 gctgcaggtg ttcccagata ccgttttgtg tacgggagct ttcttttccg gagagttcca
     5401 gggatatttc cggcatgcgt tccgccaggc gcgggatgtt ttcctcacta tagtaaaggc
     5461 gaatccgtaa ccggtctcct gccgtgtccg cccgcacgga gtatccctgt gggggatttt
     5521 tcctgttgag ccaccgggcc agggtttgga taaggtctcc gtagccgtcc cattgctgga
     5581 tgttttttcc gagttctccc cccatggcga acgtaacggc ggccgtccgt cccgtccccc
     5641 tgttccagaa ggagaccagg ggcgccgcgt ttccgtcttc cgttacgcag gctgctgttg
     5701 ctccattcct taaataacac aggttataac cgtctacggc agggggccat tccggctggc
     5761 cggctgcaat ttgaagccag ccagccgtgc cgcgcaggag ggtgcgttcc ctgatgaagg
     5821 cggcccgggc cacgctgacg gtttcctgtg caaagatgct ggggatatcc cccgggcggt
     5881 cgcagaaaaa gatgcgcccc cgtcccaatt ccgctatttc cctgagcagt ccggcatcgg
     5941 cgcttttttc cgttcccagt gcgatgacgc ttacggtagc cccttcctta accatttctt
     6001 tcagagtttc ccggtagtcg gcaggttctt cggaatcgtc ggcatctgca aagaggagca
     6061 gatgccgtgt tccggccacg gaacgttgca gttcctgcca gcccgctttc agcccggcgc
     6121 cgatgaagat tcctccgccc atagaggaaa tcctggagac gctggatatc attttcttcc
     6181 ggttagggcc cagactgctt agcgtgacaa tagggtgcgg ttcgctgtcc acggcgtgga
     6241 cggagatgag atcctggtcg gacagcaggg aaatggtctg gcaggttccg gcattggcca
     6301 gatccatttt cgtctttcct ccgggaacgg aacaggacat ggagccggaa cggtccagaa
     6361 cgatgctcat ggccgtcatg agcttcatct tgtccttctt catttccatg gaaacgggga
     6421 gcagttcgtc aatgggggag gagaagtagc cgccggagcc gaaactatgc ctgcccccgc
     6481 acatgagcag gccgccgccc tgttccctta cgtaatagtc cagggcatgc agaaagtccg
     6541 gcgggacgga ggatgcggag aggttgttga tgatgaccag ccccacgccg gagaggtggc
     6601 gggcatcaag ccggttcagg ccggacggtt gttggacgcg gaacccctgc gcttccagga
     6661 aaggcgccag gggatcgcgg tcgtagccgg agagcagcag gacgccgttt cctcccgtca
     6721 cttcaaggaa gctgacggca cggttgtttt cagggattgg atcgttttgc ggcgttatgg
     6781 tcatttcata agcggaacag ccgggtgtgg agagtctgtc cgccagcctc accgtggctt
     6841 tgccgttgag cagggtagcc gtcccccgca gcggagcccc tccgtttttg ctgatttgcc
     6901 acggaacggt gatggccggg ccgggagggc ctgccagggt tgcctctaaa ataaaagctt
     6961 cgccgggccg gatgcgcgcc ggggttcgga tttgttctat gcggatgtcc gcttcccgga
     7021 aggtgacgga caggcggtag tccaccggaa tccgggactg gatgagctgt tccgccgcct
     7081 ggtccagcgg cgtggtgggc cagccgtccg tcaacatcag gatgcggttc gtccggttcg
     7141 gggacatcag ggccatggta taggccaggg cctgttccat gtccgttccg gcggttcccc
     7201 cggcaaagac ggggtctccg cgtccccgga gcacggcgga ttgcgcgaaa tccaccaatc
     7261 ggacgcggtc cccggcatgt ttgtgccgtt ccagaatagc ctggatttcc tctgccccgg
     7321 ctgccggaat tcccgccgtg gaatttgatt ggtccaccag cacccacaag tccatgcctt
     7381 tgccgcccct gtcaagggca ggctgagcca gggccagcag caggcaggcc tgcagaagga
     7441 ggcgcagggg ggaggccagg cgcagacgcc tgtatttcca gcctgccgcc agaagcaggg
     7501 ggatcaggaa gaaccattcc ggggtgttga attgaatcat gggcgcaggg agatgtctgg
     7561 gacgggagga agccaggaaa gaatcagggc caggaacgcc agcgccagcc agaggggggc
     7621 cagagggtca cgtttcatgt gccggagcgc ctcttcatgg ggctggggaa gtcctgtgtc
     7681 aaacgtggag cagtgggaaa aatctcccat ccgggcgtcg gagaaccaga cggagccttg
     7741 gaacaggggc gttttccctt ctccgggggg gaagatttcc acatatccgg tttcttccgg
     7801 gagcctgccg ttaaagacag tttcacggcg ttctcccccc ggcatggttt gaatgagttt
     7861 tccgcctgcg ggcatggata agagagttcc tccgggaagg ttgccgtaat gtgttccggg
     7921 cagattctcc tgtacggatt gcataaatct gcgggtcatg agcaggggcg ccggaacgcg
     7981 gtccgcattg gatttttccc agggccagtt gaggaaaagg cgtttcccgt ccacccaggc
     8041 caggggggat tccccctgcc agagcaggac gccggctttt tcccccggct tcatggaacc
     8101 gatggagggg atgagcaggc cgctccagtt cagcccgtca gtcagcggat gccgttccac
     8161 cgtgacagcg ccgtaagatg gcttgccgct ggctgcaaat atgatggccg cttttccggg
     8221 ggccttggcg ttttccgttt ttccctccag caggaggagg tcgggatctg aaccgtccgg
     8281 gacgggggag aatcccggca ggctatggat gatattgagg aagatttttc cggattgttc
     8341 cggaattccc atgctgacgg caacgggggc gggagcggag cggaccagca ggagctcatt
     8401 gtccgccggg aaggcgtccg tcgggagccg gagcacggct tttccgcatt caggagggag
     8461 gctgtattca aattccgtga cagttcctgg attcaggaac agggaacgcc gggcaggagg
     8521 acgcccgcct tccgtatgga tggagatttc gctccgaaga ggggatggac tattgttttt
     8581 aacggcaatc ctccaatggt cggggccgac ggtctccacc ggggttattc ccgcaaaacc
     8641 cacattttcc agaagtttcc ctacaccccg tgcggattgc ccggcgggaa ccctccgggc
     8701 agtggatgta atcaggcgcg tgatgcccgt tggtccggca acggctgtgg cggtgcgcag
     8761 gacaggggcc aggtcatgtg ttccttcgcg cggctgccag agatccaggg cgtgcagggc
     8821 ctggcgggcg gacggccccc ggtagagcgg ctgtccggaa gctctgctgc ccatgagaac
     8881 ccatgtggcc gggattccgg accggaggat ggcgtccatg tcttcggaaa cggcattcac
     8941 ggcttcttga cgaaaggggg tcatgtttgc ggaatcatcc agaatgaaga ctacggtctg
     9001 gccggagtct ttgccgggcc aggcgggagc ggccagaacc caggtaagca ggaggacggc
     9061 cagaatttgc atccagaagg agcgcgatgc gcgcaacctg tcccaggcat taccctcatg
     9121 ggcctccgga accagcgctt ccaacaggaa gagcgtggct gtggcctgtg tttttgtttt
     9181 gtgctggagg aaatggatgg cggtgatgag ggggatggcc agaagcgccc agaacgcggt
     9241 cgtgttggtg aagtggggga tcatgggctt ggggggagag taaagggtgc gggtgcggtt
     9301 caggagagtt ccacggcccc ctgccgtaat gcttctccgg acagggcttc cgtcagcgtc
     9361 cccttgcagg ggacgcgggc aagaacggtc tggcggcaca ggcaggcaga aatccagagt
     9421 tcaaaatggg cggcgtaggc gcgggcgtac ctccgggaca gggaaggggt gatgagctgg
     9481 cggcgcaggt ggccggattc acagtttttg agtctggcgt tgccggcggg gagttccgct
     9541 tcttcctgca gcgtgggggc caggatgacg gacatccctt ttaaggaggc catggtttcc
     9601 aaaaggttgt ccggttcccc cgggtagagc aggtcggaga tgaagatttt cattccattg
     9661 gggcgccaga tggaaatgga gggcatggat tcatccgggg gaaggctctg gagctgcgct
     9721 tcccattggc cggagagaac atcttcctgg tccaggggga tgatacggct ccctttcacc
     9781 gcatgaatct tcacctgggc tcccgttccg atggcggaga gcaggcaaaa ttgaagcagg
     9841 ccccgcgtgc gtgccgcgcg ctcttcgtga aaaaacatgg attcggaaac atctaccgcc
     9901 acgtctacct gcggtgacag ttccgcccgg aagactttca tggtgagctg gccggttctg
     9961 gcgtacgccg cccaatggat atcccgcggg tcatcccccc actggtagga gcggcttccc
    10021 tgaaagtcga tggagttgcc tgctcccgtt ccctcccaat ggccctgtgt tcctttccag
    10081 gcgcagcagg aaaagggcag gcggagacgg cgcgccgcct gttcggtgtc cggctggatc
    10141 acctggctgg ggaaggggaa agaagtcatg gggatggggg agaggcaaaa cggagggcaa
    10201 gaactattcc cgttccctgc gggaatttcc cggtttctgg agaggttcgg gggatgcgtt
    10261 ccggaatatg tcaatccagt gtttgctggt gagagccaga accgctattc cggcagccag
    10321 gtccatcagg ctgaataggg aaaatttgct gaaagccggc aatgtttctt gtctcaaaag
    10381 ttcagataaa taccctccgg ggagaagggg gacggacaga ttcacgcccc acagcacgtt
    10441 ttgcaccagg cagaggatga ggcagatcat ggtgtacgtc agcagggtat tgtttttcag
    10501 tttttcccag aacggtttca gaagcatggc cggcatggtg atggcgtacc agccgcctgc
    10561 tgcgaggcaa aagagccgtt gaggggactg gaattcgggg atggatgcca tgcggtctat
    10621 cagatacacg gcgatagccg ccagggcgca gagcaacagg gtgaacagga cgctccccgg
    10681 catgccgggg aggaacagca gctgctggag cctggagaat cttttgtttt tcatttgctc
    10741 catgtgaatg ggaagaaggc gggcaggcgt gttcaggttg ataatcatca gggaaattcc
    10801 gtaaaccatg acaaaaatgg aatttccggt catgatgttt tgaatggagc cgcttgcgtt
    10861 ttccagaaag gggaggggga ggatggaggc tgcgaaaaaa gccagcagaa gcttccgcag
    10921 gggggcggcc gtgttttgtg cggtagggga gaaccattcc cttgccagca gaagcaggga
    10981 tgagatgacc aggaaggcgt ccgccaggac gaggaatatc aggagaacgc cttctcccgc
    11041 tccatctcgg aaaaaatcca gattctcatg gctgcctccc agggtcaata taccgatgcc
    11101 catgagggag aaagcgatga tgcgcatcag ggagggcatg gcggacgccc acagggtata
    11161 tgcggtcagc atgccggagc ccaggtaaag gaggaaaaac agggttacat cctgtaccag
    11221 gttaatttcc ccgtagtaat accggatgag aaagaagggc agggagataa aggcaatcag
    11281 cagggattgg gccatgaagg atatccattt tcccagcacg atgcggcctg cgctctgcct
    11341 ggtgagcagc agcagttcgt tgtttcgggt gagcagttcg ttcctgatgg aggtggaggc
    11401 cctcagcggc atgatgaaga gcagggcgac gaccatggta acccagaaga tgatctggca
    11461 tccgtcgggg ctgatgaagg gctccccatc aagaggattg atgatgaagc tgaacaggaa
    11521 gaaaaaggag agcagggagg gcactatgct gaccaggagg atgaatgacg gcgttctgag
    11581 accctggcgc aattccttga ccagagcgga gggcatccat atggggaaat caagagagcg
    11641 ggaggtattc atgaggcggg gatggatggg ctttcctggg aagaggcggg attctgccgc
    11701 ggggccggtt ccccgctgcc gtggataagg atgtccacaa aagcttcttc cagacggcgt
    11761 tcgctgcggt gaaaatcaat tacggggagg tcgaggcaga gctggcggag ttccgcagcc
    11821 acagcttccg gttcgcagga gacgaaggcg gccactactt tctgttgctg tacatggcgc
    11881 acgttccatc cccggcggag ggccagccat tcctccagtg aggcggtgtt gtctccggcc
    11941 gtgcggattt caaaggcgta tccggattcc gtctggcggt gcagcaggtc ttccatattg
    12001 ccgtcatgga tgacggttcc tgcattcatg aagatgaggg cgtcgcacat ttcccccagt
    12061 tccgagagaa tatgggagct gatgagcagc gtttttccac gcgctttgag aagatgcacc
    12121 agatttttaa attcaaggcg cgctttgggg tccaatcctg ccgccggttc atccagaatg
    12181 aggaatttcg cgtcgctgat cagcgtgcgc gccaggcaga gccgctgcgt ctgccctttt
    12241 gaaagtttgt caatcatctg gttctggagg ccggccagtt ccgtgaagtc catcacttcg
    12301 tccacggcgt ccagtaaacg ttccctgccc atgccgcagg ccctggcgaa aaaatccaga
    12361 tattcccgga cgctggtgtt tttatagggg tggaaatagt ccggcatcca tccgatgcgg
    12421 gaacgtgcca gttcgggatg attcacaaca tcggccccgt tgagcctgat gtggccggag
    12481 tcaggcattt ccagcgtggc cagcatgcgc atgagggtgg ttttgccggc tccgttggag
    12541 ccaatcaggc cgacgacctg gccctggcgg atgacgaagg aggcattgtt gacggctttc
    12601 agcggaccga atgatttgga cagtccggaa acttccagca gtaggggcgt actcatggtt
    12661 attcgtgcgg ctggggagtg acggggccgg agacggtgac ggaggttttt tcccagttaa
    12721 tggaaagcag ggtggggatg gggcccagtt ccccgcccgc cggttccatg gatgcccgga
    12781 agtgcagcgg gggagggccg tccggttctt cttccggcag gaacggggat gcctgcattt
    12841 tacgaccggg aggcagttgt tcaagagtcc agtattttcc ggaggcgtcc cggtaggtga
    12901 gtccgttcag ggttccggga aaggtgcttt ggaatacggg cgccccgccg gggctcgtct
    12961 ccagcagagt gagcgccgcc cgtgtggaaa ccggcatgat gatccggtgc tggagcgtgg
    13021 agcgactggt gaaccatttt cccccgcatt cttccccttg ccgcgccgcg ccttctatgg
    13081 gtttttcctt aatggagccg cgcgccttgc tcatgcgtgc tccggtgatg ccggcgtttt
    13141 ccggcaattg aaatgtattg ttcagcagga cggaggtctt gcaaatctgg ttttgggaga
    13201 tcagggcgga gtggtcctgc ggattaacct ggataagaac ttcccggctg cccgtccctc
    13261 ccgtgccgtc tccggccaga atcaggagca gcagaagaag ggaaaatccc acggaaatgg
    13321 ccgggatcag caggaacagc ctttgccttt tgcctgcggg agcccacagg aacaggctca
    13381 gcgggccgac aagcacggca aagatgatca gccccagccc cagcgtggtg gatggggctg
    13441 agaaataata gggagcggcg gaggtaatct ccgggtaaca gtttcccttt tcgtggaggt
    13501 tgatgaattc cttcagtttc agcttttttt cttcttccgg caggccgtcc agggacggaa
    13561 cgtgcagaat gcgcccgttt cccagcaacc gggtggaaac ggaaagcccc ctgtcaccga
    13621 tgagccacag gtttccccct cccgtcaccc attggcggat ggctttgcgg tgggcttcgt
    13681 caaaatgggt acggtaggtt ttttccggga tgatgaggca ggtctgcccc tcgtacatcc
    13741 ggtagtcggc tggccatttc atcaggtcgc aggaggaaat ggccgcgtta taggaagatt
    13801 cggaaagcag gtgccagtcc gccggagcgc cttcctggat aaccgcgaca attttcctgt
    13861 gctccgtatc cgtgggaatg ggggaagctc cttcgtggtt ttccttcact tcgtagacga
    13921 gattgtagga gaggagaggg taaacgactg catgaacgct ttcccgggcc ggaatgaaga
    13981 acggataagt atggctggtt ctcctgtttc cgttccaccc ttcatggaat tgaagagtgg
    14041 cttttctgtc cgagccggtg gtgttttcca gcttcacccg gagagggacg cgggcgccgt
    14101 tcgcgctgta gtcaaatgct ttctccacgg aaacggcgat ggggggagcg gcgtccggtt
    14161 ttgcgtggag cgggagaata ttgaatgccg aaagaaggaa gaggctgaag aacagccatg
    14221 aacgggagag agggcggcgc atgggggaag ggctgaggag gtgtggtcaa tgaatttttg
    14281 cggcggacag ggaatccgga aggggcttgt tctgggcagg gatttccctg accaggcggt
    14341 tgatgactgc ggtgggtgtc tgtccgtcca gacgggccgt gtggttgagc aggatgcggt
    14401 gctccagcac gggatgaata acggcctgca catcctcaaa ggagcagaaa gcgcgcccat
    14461 ggcgcaaagc tcgggctttg gcggcagccg ccaagcccag ggccgcccgg gggctggccc
    14521 cgtatttaac gcctccggag gcttccgatt ctccgggatg cgtagcgttg accagcctgc
    14581 ctatatagtc cgccaccact tcgggaatgg ctacattccg ggccatttgc atgagttcct
    14641 gcaattcctg cgcgttcaaa accggggata tttcaggttc ctcgcccatt tcccggttga
    14701 ggacaatgcg ggtcagcaca tccgccggtg tgcggttgac gttgattttg aaaaggaagc
    14761 ggtccagctg cgcttccggc aggggaaagg ttccttccag ttcaatgggg ttctgggtgg
    14821 caagcacgaa aaagggggag gggagggcat gggtctctcc catgacggtg acgcggcgtt
    14881 cctgcatggc ttccagcagg gcggactggg tcttggggga ggcgcggttg atttcatccg
    14941 ccagcataat gttggcgaag acgggccctt cctggaagat gaagtttttt gttccgtccc
    15001 tttcctgaag aaccggggtt cccgtgatgt ctcccggcag tagatcgggt gtgaactgga
    15061 cgcggcgcgc tgtgatatcc agtgctctgg agaggccttt gaccagttcc gtcttgccca
    15121 ggccgggcag gccctccagc aggatatgcc cgcgagccag tacgccaatg agaaccagtt
    15181 caatgagatc cttttgaccg aacaggacct ggttcatggc ggcagccaga gcacggagct
    15241 tttgcatggc gcagccaagt tcttcctgag aaaggatagg gggggtattc atgggaaact
    15301 gccggatttc cgcggctttt gggaaaaaga ccgggcattt gttaggtttc ttcatgaact
    15361 tatgccatac atccagaggg gctgcaagca gaaagatggc ggtacctgtt aattttcagt
    15421 aatatgaaga gaaaataccc tgcggggttt gagtggtaaa tggcattgct gcaatgtatg
    15481 ttccatctgt tgaaaaaaga aggcttcctg gcatgtatta ttgtcatgcc ttccgtatat
    15541 ccccccggca acgaacgcat ttacgtggtg gacgctctgc gcggctttgc cgtaatggcg
    15601 atcatgctgc tgcattttct tgaacatttc atctacaatt cctatcccgt ggcttcttcc
    15661 ccaatgatgg aagcggccaa ccagcagttc aaggaggtct tttttttcct gtttgccggc
    15721 aagtcctaca ccattttcgc ccttctgttc ggattcactt ttgcggtcca gtaccgtaac
    15781 caggcccgga agggaaggga ttttgcgggc aggtttgtct ggcgcatgtg cctgctggcg
    15841 gtgttcgcct gcatgaatgc agcgtttttc ccgggcggtg atgttctgct gactttttca
    15901 cttgcaggtt tgttgctggt gccgtgccgc cggttgaaga catcctcgct ggtggctttg
    15961 tccctgtttt tcctggccca gccgctggag tgggcctatg cggcggttca gtggatgcat
    16021 ccgggctggg ttcctccctc cctgtctgtt gcggaatcgt acgcttccct gaaatccgcc
    16081 gtggatacgg gaaatttctg ggtgatggcc tgggagaacc tgactacagg acaatgggcc
    16141 agcctggcat ggggtattga agccgggcgt ttgatgcagg ccccgggcct gttcctgctg
    16201 gggtttgttc tggggcgcca ggatttcttt ttgcagaatg acggaacggc tgtgttctgg
    16261 atccgcggac tgctgttttc cctggccggg gcctgtgcat tttacgtggt gatgtgcctg
    16321 ccggggctgc cggggcctgt gcataccgtg tttagcatgt ggcacaatgt ttgctttacc
    16381 ggcgtatggg tggccgggtt cgttctgctg taccggatgg aatatttcag gaaagcgacg
    16441 gctccgctgc tgacctacgg gcgcatgagc ctgactaact atgtttccca gtccgtgatc
    16501 ggctccctga tttttttccc gtatgccctg gggctggctc cctactgcgg gtatctggcc
    16561 agttttgtgg tcggctttgc cgccatgacc ggacagatat ggttctgccg ctggtggctg
    16621 gagcggcacc gctacggggt gctggaagga ttgtggcacc gggcgacatg gctggccgcc
    16681 gggaaggttt ccgctccgga acaccggtaa gaaacggggg ccggcgcaat gatgtttccc
    16741 gatacttgcg ggacgctttt tagcgtcttc tgccaaagcc ggtgatttat ccggacggag
    16801 gatgcatccg gttccgcaag gggcattttg gagaggagac atggaacagg gggcagaggc
    16861 aggaggaaaa gggatgaacg tttcatggcg gcgggagaca tgaaagcgcg gtattttttg
    16921 aagaatggct ttccgggaga tttttcccac agggaacggt ctgttttcct ggggatggcg
    16981 tgccgcgccc tctccggtcc gtgttccggc ggcttttccg acggactgat gcaggagggg
    17041 attccgtacc agatggcgct tttctttaaa ggggagatgt agtatggcat ggtgtatgga
    17101 aaggaggacg ggcataggcg gttttttcgg gctggaattg ccggagtacg gcaatttccc
    17161 gcaatggcat cccggcagga gtgtagccgt gaattccggc agacgcgctt tggaatatat
    17221 cctgcgccgc ctgggggatg tgcgctgtgt ccgtgttccc ctgtatacat gccggacggt
    17281 ggtggaaacg atggaacggc ttggcattcc cgtgattact taccgcattg acgaacgcct
    17341 ggaaccggag gaactgccgg aattggaaaa gggggaatac cttttgtaca ccaattactt
    17401 cggaatcaaa gagaaatgcg tggaccgtct ggcttcccgg tatggcggca gcctgattgt
    17461 ggacaatgcg ctggccctgt attctccggc ccgttccgga gtggcctcct tgtacagtcc
    17521 gcgtaagttt tccggtcttc ctgacggggg gatcgccgtg atggatgcgg agcggtcctt
    17581 gccggaaccg gaggagcggg acgaatccct gcctgccgcc gcttttttgc tggaatgcct
    17641 ggagcatggg caggaaaggg cttccgccgc ctgtgaacgg aatgaacggc gcttgcggaa
    17701 taccccgctg cgaagggttt cccggctgac ggagcgactg atgagcggca ttgattatga
    17761 acaggcgcgg ctgctccgca tgaatcattt cctttttctt catgaaaggc tgggccattt
    17821 gaaccgtctt tccgtggagg cggattcctt gtccgctccg tgttattatc cgttttggac
    17881 agcttttccc gagctgagga acgtgctgat tgataagcgc gttctgattc ctctcctgtg
    17941 gccggaggta ttggaacagg cccctgccag cagcctggaa cggaaactgg cgctgcatct
    18001 tctgccgctt cctgtggacc agcgctgcgg gccgcaggac atggaacgca tcgcccggat
    18061 tgtggaggca ttctattcct gaagagaagg gaggattatt ttgttagaca gttggataag
    18121 acgttgacga tacgtgaatg aatgttctaa catttttggt gtgtccgact acctttcctc
    18181 tctgggctat cctttccgtg gaaggcccat ggtttttgtc ttttccgtca tgatggtatg
    18241 gggcatgttc ctggcggccc gggatgttct catcggatgg attgccgcct tttgttttct
    18301 gctgcctttc atgtgcgcct ttttcatgaa aatggtgagg cagtccgcca tgggagaaga
    18361 ggatatctgc ccctttcctg aactggatga atggcttgac tccctcatgc tgccttttgt
    18421 ccggctggtg gtgtgcatca tcgtttcctt cctgcctttc gccgtgtatg tgcatactgc
    18481 ggcctgggcg gcggatactc ccctggcgtg gctgttcctg gcggcgggtg tgatttactt
    18541 tcccggcgta ttcatgagaa cctccgttcg ggaacgtttt tccggtcttt ctcccgtggg
    18601 ctggtggggg ctggtcagaa gggctccctt ttcctatgcc gggttgcttg gcgccatatg
    18661 cgccggggga tggatcatcc tgaaccttcc ttcagggcct ttgatggatt tcgccatggt
    18721 gcccgtgaag ctgtatgtcg cctgcgtgtt gatgaatatg tgcggactgt tcatgctcaa
    18781 gcatgaattt gctggggagg aagaggacgg cagcttttcc ccttccgggc ctgcggcctc
    18841 gtgatgctgc cgcctttccg gcgggccggg aacaggtgag tttatttggc gggttccttg
    18901 tccaggttca ggatacccag tacagacatg ttgccttccg catcttttcc ggaggggaga
    18961 tagaccttcg cattgttcag catgttttca aaggccttgg cgcggagcat gttcggtccc
    19021 atggcctttt ccttcaatgc cagtccttcc gcttccgccc gggctgcttc cagggctgct
    19081 tccgcctggg ctttgccccg catgacaatg actttggcct ccgcttctcc ccgggccagg
    19141 ttagcgtttg cttcctggcg cgctatttcc attttcatgg cgccttccgc ctccccgcgt
    19201 gcacgctgga tagcgatgtc tttccgggcg atttgaagtt ccgtttcctt gcgttcgttt
    19261 tcctgagtcg tctgaacttt tctgacgacg gattcaatga tggcctgcgg gggatttgtt
    19321 tcccccacgg ctacggaatc cacaataaag ggggtgccgt ccagccgctt gcttaactgc
    19381 gtgtaaacgt tgtcggaaat tttttgcagg ctgccggagg cgtccagggc attgtactgg
    19441 cttaattccg cccgcacggc tgtacggaaa ggctgctgaa tgaaattttt gtaggcaaag
    19501 agcatgattt cgtccgccac ttcgtcatca ttgcggccgg agctttgggc gatgcctccg
    19561 taatcttcca tgaattcctt gacccgggac gggtccagcc ggtaaaccag agaagctttg
    19621 caggaaatgg ggagcttgtc cttgcccagg atagcccctc cattgcgggc gtcgaattct
    19681 tccaccgtgg tatccggagt gatggaaacc ttgctgactt cctttctcca cgcccatccc
    19741 gtggaggacg gcccgatcag gatgccttca aacctgttct gtccgaaaat gggtttggaa
    19801 tagacatatc cggcaaagcc ggacaatacg cgcttgttgc tgaaattctg gaaggcaaaa
    19861 atggcggcgc ccgcagcgac gaagatgacg gcgggaatga gcagggagaa gacggttttg
    19921 ggagatttat tcatgacagg gcagcggttg tgacggtatc caacataaga tgaggctggt
    19981 gaaaatcaag ttatcttttc ctgtgcggcc gggaaaagtg ttccactgaa aaaattcttt
    20041 ccggaagagg gaatgcggca aagtatgtcc tcttctgaac acaggggagt gccggaaatg
    20101 tcttatccgg caaaggctgc ctgttccgtg ggggagattc cggcgaggtg ccggacctcc
    20161 gccccggagg cgggcctcca tttgggttaa ggtgatgaat gtagccatta cgggatccgg
    20221 aggatttata gggagtcatt tgtccaagcg tcttgagatg ttcgggcatc gggtgacgcc
    20281 cttgcggcgc tatctatttg cccaggaggg cgtggattgc ctggcgcagg ccctggcggg
    20341 atgccatgcc gtcatcaatc tggccggagc gcccatcgac cggcgctgga cggatgccta
    20401 caagagggag ctggtggaga gccgcatcat gaccacccgc aagctggtgg aagcggtcaa
    20461 caggctgcat gaaccgccgg gagtgatgat ttccgcctct gctgtcgggt actatggctc
    20521 gtccggcggc tgtcatgggg aactggacgc tccggaagcg gattcctttc tggcggaatt
    20581 gtgcgtggcc tgggaaaagg aggccgggct ggtgaacagc tccgtgcggc tggtccttac
    20641 ccggtttggc gtagtccttt ctccggatgg aggggcgctc cccgagatga tgcggccggc
    20701 ccggttcggc gttacggcaa ccgcaggaaa tccggatcat cttttttcct gggtggcttt
    20761 ggaagatctg gcggatgccc tggtcttcat tatgggcagg cagtatattt ccggcccggt
    20821 caatgtaacg gcgccggagc gcaccaccaa ccgggaattc tacagggcag ccgcggaaca
    20881 tttccacacc cgtctgtcca tccgtgttcc ggacgccgtt ctgcggttgc tgatgggaga
    20941 agcttcccag gtgattaccg gcggccagtg cgccgttccg gagacgctga tgagggaagg
    21001 gtttacgttc caatatccgg atatccgcac tttttttaac aaagcatttt ctgtcaaatc
    21061 atgagcatga tacagaccat cgttacttcc ctgtttcacg gtcattccgt caaaaccgat
    21121 cccatgccgg atttcaatct ggaacgttat ctgggggaat ggcacgaaat agcccgtctg
    21181 gaaaactggt tcgagcgcgg gctcagcagg gttcttgccc ggtatgaccg cagggaggac
    21241 ggttccattt ccgtggttaa cagcggttat gacgttcgta ccggggaacg gaaggaggcg
    21301 cgtgcacggg cggtggcggg ggatgccccc aatcatttga aagtgtactt tgtaccactg
    21361 gtatacggcc ggtatgaagt ggctttcctg gatgaaaatt acacccgcgc cgtagtttcc
    21421 ggcggttccc tgaattatct gtggctgctt gcccgctcgc cgcagttgaa ggatgacgag
    21481 cttcagccga tgcttcattg cgcggaaaaa ctggggtatg atacttcccg gttgatttac
    21541 gccaatgtcc aggcctctcc ggacaaatga catggggcgg gcttgaagaa gcggtgtgcc
    21601 gtgaatatgg gatatttttg aaatggggaa tgtggaaaag gtggcttttc tcctgccagg
    21661 aaaaaatttt tgggcatggg ccgggcgttc ctttccagtg ttccgccgtg cgggaatgtc
    21721 atgcttttga attctccggt gtggttcttc ttcctgcatg gctccggtcc cgtatttttc
    21781 cgctttttcc tgccccgtcc tttccgtttt tttgcaaaga acagggaaaa acacaggcgc
    21841 ctgcttaaaa taggcttgca atcatgagaa ggatggatat gttgacattc atccaaacat
    21901 tggatgtttg gatgaattac ttaaagaagg agtaacaatg gatattattc atgacaacgc
    21961 cgctgatctc agcgcattga acggcaagac cgttgccgtg atcggatatg gcgcccaggg
    22021 gcgcgctcag gcactttgca tgcgtgattc cggtgtgaac gtgattatcg gcgttcgccc
    22081 cggcaagtct tttgacgccg ccgcccagga tggattccag gtaatgagcg tcgcggaagc
    22141 cgccgaaaaa gcggacatca ttcacattct gctgccggat gaaagccacg gcgccgtgta
    22201 tgaagctgaa atcaagcctc acttgaaggc cggcaagacg ctttgctgct cccatggctt
    22261 cgcgtatgtt ttcaatacca tcgtcccccc cgcggatgta gatgtcatca tggtggcccc
    22321 caagggcccg ggcacggaag tgcgccgagt atttgaagaa gggttcggct gccccggcct
    22381 gattgccgtt catcagaacc cctccggcaa ggcccgtgac gtggctcttg ccatggccaa
    22441 ggctgaaggc ctgacccgcg gcggcgtgct ggaatgcacc atggcccagg aaacgtatga
    22501 agacctgttc ggtgaacaga acgtcctgtg cggcggtctg gtggacctga tgaagtacgg
    22561 ctttgaaacc ctgacggaag ccggctaccc gccggaaatg gcttacttcg aatgcgtgca
    22621 tgaagccaag ctcattgtgg atctgattta taacggcggc attcagaaaa tgaactccgt
    22681 gatttccaac acggccgaat tcggcgaata ttacaacggc ccgcagatcc tgcccgcgga
    22741 ggtgaaagaa cgcatgaagg aatccctcaa gcgcattgaa tccggcaagt tcgccaagga
    22801 ctggctggaa gaggcagcca agggcgctcc caacctcaaa gccaagcgcg aagccctggg
    22861 ccagcacccg gtggaaatcg tgggcgccaa gattcgcagc ctgtttgaaa ggaactaagg
    22921 tttcttttac ctggaacgcg tgccgttccg ccggaatgcg gcggagcggc acggatattc
    22981 ccgggctttt cccggaaatc cgccatttgt tcagtttatg gagcacgcca cgccggccgt
    23041 caatgtggaa aacgccaggg tatacctggg cgggcacgaa atcctgcaca atatttcctg
    23101 gcaggtgcag cgcggagaac gttgttttat tctgggggcc aacggcgccg gaaagacaac
    23161 gctggtcaag gtgctgatgg ggtttgcgtg gccgctcttc ggagccaggg tgcaggttct
    23221 gggcaagacc ttcgggcacg tgaacctgct ggaattgcgc aagtccattg cgtgggtgag
    23281 tccgttcatg cacaagtggc tggaggatgg ctcctggaac gggcgcgaca tggtgctttc
    23341 cggccccgac ggcactatcg gcctcttgag ggagccgacg ccggaagagg aggaaaaagc
    23401 cgcaggaatc atgaaatccc tgaaagcgga gcatttgata gacagaccgg tggtagccat
    23461 gtcttccggg gaacaggtga aagtgctgat tgcccgggct ctgatgacga atccggaact
    23521 gatgattctg gacgaaccca gcgtttatct ggatttggca gggcgggaat ttttattgaa
    23581 aaccattgaa gaactggctg aaacgaggcc tgatctgacc atcgtgttca tcacccagcg
    23641 cattgaagat atccttcccg tatttaaccg cggcatgatt ttaaagtctg gagaaatcgt
    23701 ggcgcatgga agccgggacg aggtgctgac ggaggaaaac ctgaaagacg cctttgggct
    23761 ggatatccag ttaatcaaga cggagaaggg gcgtctctgg accgttatcc gataattttt
    23821 catgaagcag tccaagacaa tggagctgaa gaaaccggtg cgggtgtccc tggcccgcca
    23881 ggtgcttacc gccatggaat ccatgatacg gtccggcaaa tggaaggttg gagaccgcat
    23941 tcccgcggaa gcggaactgg cccgtgcctt ttccgtcagc cacaacacca tccgcgaagc
    24001 ccttcaatcc ctgattcaca tggggatgct ggaagcgcgc cccggggacg gcacgtacgt
    24061 gatggcgtca gaccgctttg ccgtcgcggt gagcaaccgc ctcaaggaat ccgatctgcc
    24121 tcagattctg gaggcgcggc tggctttgga aaaggaaatt gcgcggctag ccgccgttaa
    24181 aaggacggat gaagatttga aggagctgga aaatgccctg gaagactgcc acggcagggt
    24241 aaggcagggc attgaggatg acatgctgtt tcatgccgcc gtggcccgcg ccacgcataa
    24301 tccggtgctc tcggaattat ataacgtggt catccgccat gtgcaggaga atctggaaaa
    24361 gctgcttcag gagaagcaat acgactccgg cgccatgaag ctgcatgacg accttctcgc
    24421 agccatcagg aagcaggagg cggatgaagc ggagaacatt attgtaaaga ttgtggaatt
    24481 tgacaccgtc agcataggcg ggtcatgccc ttcttaaatt aacggaacag gcgattccca
    24541 tcatgcggaa tcggcatttt tttcttttcg gcctttttag taaggccgtc cggagaaatg
    24601 aaattgggga acaagaaagc agaaccggca tgaataattc tgaaagaaaa agtgttgcgt
    24661 ttcgtattca ttggtaatga aggcgaatgt attttccata atgatgttgt tctttcatct
    24721 gtttccggca tatggaatcg accccagcgt gaggggattt gaagaacttc atgaagtgtt
    24781 gaaaagtgcg gttcgggaac ttgataccgt tcaatctccg gatcatcttc ctatggctat
    24841 ggagcatttc cgtgccttgg tggaggaatg tgggcgtaat cccgatcttg ttgccgttct
    24901 tgagcttact tcagaatcat gtccccctca gctcaagaag gcttataaag cggcaatgga
    24961 gttgcagggg aaattgcatg atgcctctaa acggctggc
//
LOCUS       DAOMSU010000006        21223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_60_length_21223_cov_2.886574, whole genome shotgun sequence.
ACCESSION   DAOMSU010000006 DAOMSU010000000
VERSION     DAOMSU010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 21223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21223
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60_length_21223_cov_2.886574"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1729)
                     /gene="ilvD"
                     /locus_tag="PK077_00640"
     CDS             complement(53..1729)
                     /gene="ilvD"
                     /locus_tag="PK077_00640"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HRN22839.1"
                     /translation="MRSDRVKAGFERAPHRSLMRATGMTDEDLSRPFIAICNSFNEVI
                     PGHVHLNRVAALIKEEVRKAGGTPVEFNLPGVCDGIAMGHGGMKFSLASRELIADSVE
                     TMLSAHAFDAMICIPNCDKIVPGMIMGALRCNIPTIFCSGGPMAAGMAEDGTVLDLNS
                     VFEAVARFKAGKINEAELHSLECRACPGAGSCSGMFTANSMNCLSEVIGLALPGNGSL
                     LATSEERKEFWKQTARRAVEMAKADGPLPRDIVTRDAIDNAFTIDMAMGGSSNTVLHT
                     LAIAREAGVEYDLQRINDISRRTPNICKVAPSSRFHMQDVLRAGGVSAIIHEIARIPG
                     ALHLDAMTVSGKTLGETVEGCGIADETVIHPLENAYSRDGGLAILFGNLAEEGAVVKK
                     AGVHPDMMSFRGPAVIFESQEEACEGILAGKVKSGDVVVIRNEGPKGGPGMQEMLAPT
                     SYIMGQGLGAEVALITDGRFSGATHGACIGHISPEAAEGGLIGLLRNGDMIEYSIPDR
                     TLNACLSEEEIARRRADWKPTYNRVSSSWLSRYRQVASNASKGAVLRRGE"
     gene            1946..2650
                     /locus_tag="PK077_00645"
     CDS             1946..2650
                     /locus_tag="PK077_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRN22840.1"
                     /translation="MATILIAEDDHAISDLVAYNLERAGHTPLAAYDGLTALEVARRD
                     KPELILLDQMMPGMDGHGVLRELRRDSRTQNIPVIFLTAKAQAEDRIQGLELGADDYV
                     TKPFSPKELVLRVASVLKRCEHTPGSVIAEYGPFTFDKNALKFYIDKEEVDLTATEFK
                     LMIHMVEREGQTLNRNDLLSCVWGYSHQAQSRTLDTHMKRLRQKLAPYADCIETVRSI
                     GYRFTLPGRRIPEEKA"
     gene            2663..3940
                     /locus_tag="PK077_00650"
     CDS             2663..3940
                     /locus_tag="PK077_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRN22841.1"
                     /translation="MSAIDYILTILILASLYLNWHLVQVCRSAMKARKKALRDAQRLL
                     KRGEEAQEQAIADKRRFLEALGEAFLLIGPSGHIVLANTLAKELFQEEKLEGRKVGAL
                     VCNQELLGHVQEAFDTDGPVTKEFTLSAANSPGGVQNGITAWHLDSAITDAPIREKRI
                     LLRNITQNYLTNQMRRDFVANASHELRTPLTIIVGYLENLMEDDLVEESPGLARKFIG
                     VMHQNSQRLMNIIEDMLMISKLESGHKAILKEQWFRLTSCADDVFSRLDSIREKKQAV
                     LHMDIPTDWELYGDPFYWTQILFNLVENALKQNTEPGLSITVAAAKTQDACVITVTDT
                     GVGIPAESIPFLFNRFYRVETHHSSEIRGTGLGLSIVKRAVEAHDGAITVSSIPHRET
                     VFTITIPLKRFREEKRIRQLSQVSIETSANKST"
     gene            complement(4083..5660)
                     /locus_tag="PK077_00655"
     CDS             complement(4083..5660)
                     /locus_tag="PK077_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22842.1"
                     /translation="MESLLKDRELAQACYMAHFFALVGKDRTYILHELKDKEYVLWLL
                     NHPEVFEKLSFAKASGKDTLAVLRNIWLKEGKELSGVGLNMALGAALVSSSREPEACE
                     ARYDFYKKSFMEKKLFPQFLTLEPWEFGILFRGRESIEELAWAQDYLADKKKIQAGNA
                     GYACCGLIPYRMKNKQGISVHVGGAFYDHKPVSLQIYVEYGGVCGAVSKGAAGFVKAK
                     GIPSYTIGQPGHCAFVWKGIDGEWKIGNNIYGWVWSEGGSGGPWKGAVSTITELPRFW
                     KKNAAASNLCYYLSLLAADPQKAGTLLKEALKRNASNYPAWQALTKRNAKRSEKEKLV
                     LLEQFKEAFSGNPTMWEYFLKKELGLDWKKANGYAVYPGLLAENESWDSVDAYMRNFC
                     ALARRDIPDMAGKLSYEVKTKRIFFKNWLKFYQQNKVDRKVRVQTCAVLEKALPPLLT
                     HEKTALQFLGFYGQILDLWKDKQLSARADACLTAWLKEADKAPVRKKVAEIGLKVATH
                     LEDKRALVRYAEAQEEH"
     gene            complement(6052..6747)
                     /locus_tag="PK077_00660"
     CDS             complement(6052..6747)
                     /locus_tag="PK077_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22843.1"
                     /translation="MKKIILINLILLSTGFLYAHEKDMMNKVPRDSKVNLEKNDSSSP
                     VVVEGVKNRGELDSWIENYVETNYPGYNIGDREVDLSSKKSIIETVYLKNEQGNVIEV
                     CFDITDYRKKFNELHKKEIIKISRDLRKQKERVKEDKEPGSSYDFPIELKDAKTEKEV
                     LEAQKNYFPGYQVISRATWVFGKKFIDRVRLEKEDDTRVLMFDVSAHINEYRKTHEIN
                     LKEIAPGMISVQK"
     gene            complement(6744..7184)
                     /locus_tag="PK077_00665"
     CDS             complement(6744..7184)
                     /locus_tag="PK077_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22844.1"
                     /translation="MAADQPRCRVERRYLAFFTPWGKEPPTPPDIVEEALGDMVESIL
                     TEALGAETGIVDMDHLVGASMKHTYHVHLWYAYRFVHNHKFKTPWKTGMKTHLADGMK
                     DFFDPNIEGKKVLKVPCLQQKEIFLRNLKRNLKKNVKNIIDKIL"
     gene            complement(7572..8447)
                     /gene="hisG"
                     /locus_tag="PK077_00670"
     CDS             complement(7572..8447)
                     /gene="hisG"
                     /locus_tag="PK077_00670"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HRN22845.1"
                     /translation="MSKKLKIALPKGSLQDSTVELFRKAGYNVYVSSRGYRPTCDDDD
                     LELFLIRAQEIGRYVNDGFIDCGITGRDWIYENRADVEVLTDLQYSKATSKPTRWVLV
                     VPENSPITCAEDLQGKRIATEGVGITERWLQEKGIEAHVEFSWGATEVKVPELVDAIV
                     DITETGSSIKANKLRIVDTLMTSYPQFIANRETMQDEWKKQKLERLVMMLKGALEARR
                     KVGLKMNLPAASLNGLVEALPSLRRPTISHLAEEGWLAVETVIDESVVRDIIPQLKAL
                     GAEGIIEYPLNKLVY"
     gene            complement(8513..9796)
                     /locus_tag="PK077_00675"
     CDS             complement(8513..9796)
                     /locus_tag="PK077_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HRN22846.1"
                     /translation="MRYEPLPSSFFAGNREELASRLPAGSMLILHANDVFPTNADGTF
                     ALHQNANLFYLTGVDQEETVLVMTIREGGWDEILLLRETNEQIAIWEGARLSQEQARE
                     LSGIQDVRWTDEYDALLEALVPSASMVFVEANQHPRCTCPVETRNARMTKELKEKFPD
                     AVLKNVYEIMADMRQIKKPEEIKALKKACDITNEGFRELLRFIRPGVGEWQIEGFLAN
                     EFISRGPRKFSFLPIIASGKDTCVLHYIQNDKRCEDGDLVLMDIGTEYGNYNSDMTRT
                     VPVNGKFTPRQRAVYESVLNMMTYAKKILRPGILKSEYERLVRVFAAGELVKLGLITP
                     AQVAEKPSDPPIVRKYYMHGCSHFLGLDVHDVGEANPVVLPGMVFTVEPGIYIAEEGI
                     GIRLENDVLIGETENIDLLGDVPLLPDDIERLMAR"
     gene            10168..10827
                     /locus_tag="PK077_00680"
     CDS             10168..10827
                     /locus_tag="PK077_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HRN22847.1"
                     /translation="MPVSSLITHPLHTDSRQSCFGGVWYNPSEQAVWNSPRLKVYVAP
                     VNIDYIKARFPKESPALAAQFRAGLQKDIRNVLENKSKQTGGRMKWQLVDRPVPGSVT
                     LRLAIVKLKPTDVGGNIMSDLVSLVSPLPGTSLILGRFMSGDVGIEGRLSNTNTDAGI
                     MEFKAYNTDPITLFSVKEFERFAFDQRNLRLFSSCIADIFKAGPASSIPKTGEFDLDP
                     F"
     gene            complement(10824..11882)
                     /gene="lgt"
                     /locus_tag="PK077_00685"
     CDS             complement(10824..11882)
                     /gene="lgt"
                     /locus_tag="PK077_00685"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRN22848.1"
                     /translation="MNPTVYVHDLDPVIWQITDSIALRWYGLAYLMGFIGGYYLLSWL
                     SRRKLYPVPQDRMADFVTYVAIFGVLIGGRLGYVLFYQIPNHGWSQFLADPLMVLRVW
                     EGGMASHGGMIGVGLYTFYYAWKHRVKWVALLDGLAIVAPVGLFFGRMANFINGELYG
                     RIVPPGSSQGMIFPAELSQDPDLFVRVASRIYETPGLLDKLSLSGIAVPERMTAAWVT
                     DRVRDTPAIREIVGQMMQDHARYPSQLYEAFAEGVLLFAVLWFVRVRFPRAWNGLFCG
                     IFAVLYAAGRIICEEYREPDSPFSMGLTRGQFLSVFLVLVGAAFFVYAFKTRQTVQEC
                     AFYEPEKKDGKESSGKAV"
     gene            complement(11894..12973)
                     /locus_tag="PK077_00690"
     CDS             complement(11894..12973)
                     /locus_tag="PK077_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRN22849.1"
                     /translation="MAIDTDLLKKFSEAHGISGHEDEVRSLVAQELEGYGEFSSDGSG
                     SLFCTGGGAGPRVMLAAHMDEIGFLVQNIASNGFLQLVGIGGWWPHTLLSQRVLVKTR
                     SGRGIRGVIGSKPPHFLPEGQRNSVMSMEALFVDVGAESAEQVKNEFGIHLGDPVVPD
                     VKFSPLENPFRVMGKAFDNRAGLSVMIEAFEKLCREGHPNTLIAAATVQEEVGTRGAR
                     TAGVAMQPDCVIVLEGPPADDTPGFAVTDSQGALGGGVQIRLFDPTAITNPRLAALAE
                     KTALDAGIPFQLTVRRSGGTDAAALHLSGKGVPTIVLGIPTRYIHAHNGVLDLRDYRA
                     AVELTVALARSLDQEAVEALTHYLP"
     gene            13167..13883
                     /locus_tag="PK077_00695"
     CDS             13167..13883
                     /locus_tag="PK077_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22850.1"
                     /translation="MKSILNTITAMLAAVLFVPAASAQTTSNPRMQVRVSLEKLSLYM
                     RQSPNVLTQDDPRPLPKPKKWADFEIPFKVEAAPTPKSGYIDALTFKFYIAVVNPDRA
                     RQYLKLYKEVKYVNVPVGENTYASVYLSPSSVKRITGVEGGRGKWVKYQGVVVEYNGK
                     IVATYSSERGKMEKWWTIQSPSIVETSYYPLLNKDETPFSVFWYDRYPEIMRPNSQQA
                     ASGSVPAPFGTPVIPPADGE"
     gene            13930..15768
                     /gene="pilM"
                     /locus_tag="PK077_00700"
     CDS             13930..15768
                     /gene="pilM"
                     /locus_tag="PK077_00700"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRN22851.1"
                     /translation="MANSRPIVALNVGSQRVSMGVFSKTSKDALILDRYATRLVVLDP
                     SAEGLRLTKIGEAIADLVQELNVKGSVVNYSVSGQSVFIRFVKLPALDDTDVEQLIRF
                     EAQQHVPFPLDEVVWDYHLLPAKGLEREAILVAIKAEDLDSLNDEIVSHGLSTGKVDC
                     ALTSLYNAYVDSYPDEKEPVMLIDIGAKSTDLIYSEQGRFFTRSISAGGIFVTSAIAR
                     EFNISFMEAERLKTTSGLVSMSNGQTEGLDPATANLATVIRTAMTRLASEIQRTTNHY
                     RAQMSGSAPVKAYLCGGGASLPYTKEFLEDKLGIPISFFNPMHNVGVGSGVDVNTISR
                     EAFILGGLIGTAIHSIGRASLNIDLEPTAVAKKRANQKKMPAIIVGAAIAVLGAAAYA
                     VTGYMGEKKAEEILAGVQPTVTSIKSAQSALRQKEQELKKLDSTLASYQQLTLQRYGY
                     ADIIRHLLEQSEHKDYPYWFTDFEPLAHFNPEDTTQITGYSVIKDSFTSDKNTSLVDD
                     IRTEASANASSEDEAGVYSVNAIRLTGFVRRSLGGQRIIQDLQAKIDGNKESLFTFKP
                     GDVKLEARQIMELGAKDAKVDAAAGAFVPFKLVLPLKTPIPVNFNK"
     gene            15798..16751
                     /locus_tag="PK077_00705"
     CDS             15798..16751
                     /locus_tag="PK077_00705"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HRN22852.1"
                     /translation="MSNWITDNKPAAMVAGVGLLLFLGLSATGYIVNSKRSELDKKIS
                     IAAKEIKSANAAEITPSRSSNEELEKELNRYAKAVGSLETAYKPFLASSALVPTTPTA
                     FQNELKTFRDSLISSCKKKNILITDTSSWLGFQVYSTQAPSVQAASTLGFELKAINSL
                     VNKLAECGLSKFIKVYRPQLPIETPANNPEESDEADQAPWTPMPLEIAFQGDRESVLK
                     AMNAITGMQDYLFTVNSIRIRNERMMPPPIANPAAAKPAAAQPATGAASLTPADEATA
                     PAAPAIQQVIKPYMGKEQVFVQVSLNLVHFNQPKAQEPSED"
     gene            16787..17800
                     /locus_tag="PK077_00710"
     CDS             16787..17800
                     /locus_tag="PK077_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22853.1"
                     /translation="MSEKQNYDKILLASGIVLGLGVAAYGTLTFLGLNDKYKFTTQVS
                     EKAIEPPPGIKKAAEVGQELSASHELKPIAQETQKYVGFVAPNLWIKEGGMEPFDIIS
                     GPPIHGNIPNKWFLDNGLENEFVYSDVLTRDPDNDGFTVQEEYAAKTHPNDPNSHPPL
                     VSKLFVDEIKQFGFYLAFTQADGNDFTFKGMNRAKQEIWKNIVQTNGKFGARKNTKDE
                     PRFELVSVVKKEFKNPSLDMVETDEEAVVKDLKPTKNGQTYTIRRGTKYVIPIIDKKV
                     NLTITAGPERDTSFEVEEGSDFRIPGDAKQIYTLKTVDNATQTVTIANKTTGEQTTLS
                     KKK"
     gene            17872..20592
                     /locus_tag="PK077_00715"
     CDS             17872..20592
                     /locus_tag="PK077_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN22854.1"
                     /translation="MDHAPLYQPKRSLIALMAIAASCPFAQAGDGGAVGTSSALGSSY
                     AGPGSYQYQSSAARTAMARREAQTQEAMQLLAEGRNLYREGKYKEALDKYNAAYNMLP
                     SAPINDQRKEAIANHIGDASIAVAQEYIKVGRYDEADKLLQDAIKLNPRSAKLAKQTL
                     EYMKDPIRTNPALTPEHVKNVEKVNTLLHMAYGYYDLGDYDKAIAEFNKVLSIDPYNV
                     AARRGQETVNRRRMAYYAAAYDETRSTMLAEVDKMWERPIPMEVPTGADGTDNAPITD
                     INGATANLMKLKSIIIPSVSFEDTTVEDAIDYLRKKSIELDRTVGPNGERGINFVIND
                     SQPAAVAPAVPATDEDGFGEETAEVTEAAPAAAPQESIRTRKIGQLKLTNVPMLEVLR
                     FICSNAGLRQKVEDYAVTILPAGGNDVDLYQRTFSVPPGFQSALRTTVGDGGGEVSDD
                     PFGGGGESSSGLKPMPSIRSLLQKSGISFPEGATAFLVNGNSSLVVRNTSGNLDLIEQ
                     LIENTRGESQQVRIMTKFVEVTQENTEELGFDWIVTPFSVSNDRSTFLGGGTNYGTGS
                     TSDSFTQSPGGVTGWPVNSGSDTINGLVTGGNRTGDYAITKNSVDNLLNSTNRSEASQ
                     KNAAPGIMSLTGIYDEGSFQMLMRGLSQKKGSDVLTAPSVTAKSGETAKIEIIREFWY
                     PTEYEPPELPNSVGSGYSNGYGYGNNGNTLNGLLGNQIQPQISSFPVTPATPGVFEMK
                     PVGVTLEVVPTIGDNKYIIDLNFKPSIVEFEGFVNYGSPIQSTGVGSDGKPMSLTLTE
                     NRIEQPIFSKRSVETSLFIYDGHTVAIGGLITENVQTVEDKVPIFGDLPLIGRFFRSN
                     SDNHIKKNLMIFVTGQIIDATGQPVRGNALPTAAAPESALPASEGLLPPM"
     gene            20687..>21223
                     /gene="coaE"
                     /locus_tag="PK077_00720"
     CDS             20687..>21223
                     /gene="coaE"
                     /locus_tag="PK077_00720"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRN22855.1"
                     /translation="MKTLIVTGGIATGKSTFIRLLMEAGGARLRLFDCDAEAGRLLDG
                     GTLKAPLSSVFGPASVDSSGKADRHFLRELVFRNPESRRTLEGIIHPLLHQECLAQML
                     AARQNTEVDGFLIDVPLFFETSARYCQDAVCVVAVSRGTQKTRLAIRNGFREDMIEAI
                     LAAQRPIMEKVAAADFVIW"
BASE COUNT         5226 a         6267 c         5130 g         4600 t
ORIGIN      
        1 aaggaggaag gcggctgaaa tttggttggg ataagtcgaa taatacgagc tattattccc
       61 cgcgccggag gacagccccc ttgctggcat tcgaggcgac ctggcggtaa cggctcagcc
      121 aggaggagga aaccctgtta taggtaggtt tccaatccgc acggcggcgt gcgatctcct
      181 cctcgctcaa acaggcgttc agcgtgcggt ccggaatgga atactcaatc atatccccgt
      241 ttctcagcag gccgatcagg ccgccttccg ccgcttccgg ggaaatatgg ccaatacagg
      301 ctccgtgtgt ggctccggaa aaacggccgt ccgtaataag cgccacttcc gcgccaaggc
      361 cctgccccat aatataggaa gtgggagcca gcatttcctg catgccgggg ccgcccttgg
      421 gtccttcatt gcgtatgaca accacatcgc cggacttcac cttcccggca aggatgcctt
      481 cacaggcctc ttcctgagac tcgaaaatca cggcgggccc gcggaaactc atcatatccg
      541 gatgcacacc cgcctttttc accacagcgc cctcctcggc caaattgccg aacagaatcg
      601 ccaggccgcc atcacgggaa taggcatttt ccaacggatg aatcacggtt tcatccgcaa
      661 tgccgcatcc ctccactgtt tcacccagcg ttttcccgct gacggtcatg gcgtccagat
      721 gaagggctcc gggaattctg gcaatttcat gaatgatggc actcaccccg cctgcacgca
      781 aaacatcctg catgtggaag cgggaggaag gagccacctt gcagatgttg ggggttcgtc
      841 tggaaatatc gttgatgcgc tggaggtcat attccacgcc ggcttcccgg gcaatggcca
      901 gcgtatggag cacggtattg gaactgccgc ccatggccat atcaattgtg aaagcattgt
      961 cgatggcgtc acgggttacg atgtctcgcg gcagggggcc gtccgccttc gccatctcca
     1021 cggcgcggcg ggcagtctgc ttccagaact cctttcgttc ctctgaagta gccagcaggg
     1081 aaccgttgcc gggaagggcc aggccgatca cctcgctcag gcaattcatg gaattggccg
     1141 taaacatgcc ggagcaggaa ccggcgccgg ggcaggcgcg gcattccagg gaatgaagtt
     1201 ccgcctcatt aatcttgcct gccttaaagc gggcgacagc ctcaaacacg ctgttcaggt
     1261 ccagcaccgt gccgtcctcc gccatgccgg cggccatcgg accgccgctg cagaaaatag
     1321 tgggaatatt gcagcgcagg gcgcccataa tcatgccggg aacaatcttg tcgcaattag
     1381 ggatgcagat catggcatca aacgcgtggg cgctcagcat cgtctctacg ctgtccgcga
     1441 tcagctcacg gctggccagg gaaaacttca tgccgccgtg gcccatggca atgccgtcac
     1501 aaacgccggg aaggttgaat tccacgggag ttcctccggc cttgcgtacc tcctccttaa
     1561 tgagggcggc caccctgttc aggtggacat ggcccggaat cacttcatta aaagaattgc
     1621 aaatagcaat gaaaggacgg cttaaatcct catccgtcat tccggtggca cgcatcaaac
     1681 tgcggtgcgg cgcacgctcg aaccctgctt taactctatc gcttcgcata acaatacaac
     1741 tgttgcgggg caagtgtatc acatgacgca gggaagagaa gttgaatctg ggccagcctt
     1801 gccaaacgga aacgcctccg ttacagtatg tttgcataag aggaggcttt aactgtccgg
     1861 aaccgcctcc cgggcgggga aatgtgaacg aatcgtcaca tctaatatcg ggacaccgcc
     1921 ctttctttca tcgtaaaaaa cagacatggc aaccattctt atcgctgaag acgaccacgc
     1981 catttcagat cttgtggctt ataatctgga acgtgcgggc catacgcccc tggcggccta
     2041 tgacggcctg accgcgctgg aagtggcgcg ccgggacaag ccggaactga tcctgctgga
     2101 ccagatgatg ccgggcatgg acggacacgg cgtactgagg gaactgcgcc gggacagccg
     2161 cacccagaat attcccgtca tcttcctgac agccaaggcc caggcggaag accgcatcca
     2221 ggggttggaa ctgggagcgg acgactatgt gaccaaaccg ttcagcccca aggaacttgt
     2281 cctgcgcgtg gccagcgtgc tgaaacggtg tgagcacacg ccgggcagcg tcattgcgga
     2341 atacggcccc ttcacttttg acaaaaacgc cctcaaattc tatatcgaca aggaggaagt
     2401 agatctgacg gccacggaat tcaagctcat gatccatatg gttgaacggg aaggccagac
     2461 cctgaaccgc aacgacctgc tcagttgcgt atgggggtac agccaccagg cccagagccg
     2521 tacgctggac acgcacatga agcggctgcg ccagaaactg gctccctatg cggactgcat
     2581 tgaaaccgtc cgcagcatcg gctaccgctt taccctgccg ggacgccgca tcccggagga
     2641 aaaggcttaa cactccggtt ccatgtcggc catcgactac atcctaacca tccttattct
     2701 ggcctccctg tacctgaact ggcatctggt ccaggtatgc cggtccgcca tgaaagcccg
     2761 gaaaaaagcc ttgcgggacg ctcagcgcct gctgaagcgc ggggaagaag cgcaggaaca
     2821 ggccatcgcg gacaaacggc gcttcctgga agctctggga gaggccttcc tgctcatcgg
     2881 tccatccgga cacatcgtgc tggctaatac gctggccaaa gaactctttc aggaagaaaa
     2941 gctggaaggg cgcaaagtgg gggccctggt ctgcaaccag gaattgctgg ggcatgttca
     3001 ggaagcattc gatacggacg gccccgtcac caaggaattc acgctgagcg ccgccaattc
     3061 ccccggcggc gtgcaaaacg gcatcacggc gtggcatctg gacagcgcca tcacggacgc
     3121 cccaatcaga gaaaagcgca tcctgctgcg caacatcacg cagaactacc tcaccaacca
     3181 gatgcgccgg gacttcgtgg caaacgcctc ccacgagctg cgtacgcccc tcaccatcat
     3241 cgtgggatat ctggaaaacc tgatggagga cgatctggtg gaggaaagtc ccggactggc
     3301 ccgcaaattc atcggagtca tgcaccagaa cagccagagg ctgatgaaca ttattgaaga
     3361 catgctcatg atctccaaac tcgaatcagg ccacaaggcg attctgaagg agcagtggtt
     3421 ccgcctcacc tcctgcgcgg acgacgtctt ctcccgtctg gattccatcc gggagaaaaa
     3481 acaggccgtc ctgcacatgg acattcccac ggattgggaa ctttatggag acccctttta
     3541 ctggacgcaa attctgttca atttggtgga aaacgccctc aagcaaaaca cggagccggg
     3601 actttccatt actgtggccg ccgccaaaac acaggacgcc tgcgtcatca ccgtcacgga
     3661 tacgggcgtg ggcattcctg cggaaagcat ccccttcctt ttcaaccgct tttaccgggt
     3721 ggaaacccac cactcctcgg aaatcagggg aacgggcctg ggcctttcca tcgtgaaacg
     3781 cgccgtggaa gcgcacgacg gcgccatcac cgtctccagc atcccccacc gggaaactgt
     3841 ttttaccatc accattcccc tgaaaagatt ccgggaagaa aagaggatac ggcaactatc
     3901 acaggtatcg attgaaacat ctgctaacaa atcgacataa tggaatataa cactgaaacg
     3961 cctttaaata gaaggtttgc ttgttgcgca aattttcctt tctggaaatc attattaaat
     4021 acttgagccg attctccatc ttctgtatta caacaacatc cttgatcttc agacaaccct
     4081 gttcaatgtt cttcctgagc ctccgcataa cggacaagcg cccttttgtc ttctaaatga
     4141 gtggctactt tcagccctat ttctgccacc tttttgcgta caggcgcctt gtctgcctcc
     4201 ttcaaccatg cagtcaagca agcatcggcc cgcgcagaca gctgtttgtc tttccacaag
     4261 tcaagtatct gaccataaaa tccgagaaat tgaagagccg ttttttcgtg ggtcaacaac
     4321 gggggcaatg ccttttccaa gacggcacag gtctgaactc tcaccttccg atctacttta
     4381 ttctgctggt aaaatttcag ccagtttttg aaaaagattc tcttggtttt cacttcataa
     4441 gacaactttc cggccatgtc gggaatatcc ctccgggcta aagcgcaaaa attacgcatg
     4501 taggcatcca cggaatccca cgattcattt tcagccagca atccgggata aacggcataa
     4561 ccattcgctt ttttccaatc aagccccagt tcttttttca gaaagtattc ccacatggtg
     4621 ggatttccgg agaaagcttc cttgaactgt tctagcaaaa ccagtttttc tttctcggac
     4681 cgctttgcat ttctcttcgt caaagcctgc cacgcaggat aattggaagc attccgtttc
     4741 aacgcttctt tcagtaatgt gcccgccttc tgtggatcgg cagccagcag ggataaataa
     4801 taacagagat tggatgcagc ggcattcttc ttccaaaaac ggggaagctc tgtaatcgtg
     4861 gaaacggctc ccttccaagg ccctccagaa ccgccctccg accacaccca tccataaatg
     4921 ttatttccta tcttccattc tccgtctatc cctttccaca caaaggcgca atgccctggc
     4981 tggccaatcg tataggaagg aattcccttc gctttcacaa aaccggctgc ccctttggaa
     5041 acggcgccgc acacgcctcc atattccacg taaatctgta aggaaaccgg tttgtgatcg
     5101 taaaacgctc cacccacatg cacactgatt ccctgtttat tcttcatccg gtatggaatc
     5161 aatccgcaac aggcataccc ggcgttcccc gcctggatct tctttttgtc ggctagataa
     5221 tcctgcgccc aggcaagttc ctcaatactt tcccgccccc ggaaaagaat tccgaattcc
     5281 cacggctcca acgtaaggaa ctgcggaaac agttttttct ccataaaaga ctttttgtaa
     5341 aaatcatacc gcgcttcgca tgcctccggc tcccgagatg aagaaaccaa cgccgccccc
     5401 aatgccatgt taaggccaac cccggaaagt tccttccctt ccttcaacca aatgttgcgc
     5461 aggactgcca gagtatcctt tcccgaagcc ttggcaaatg acaacttctc aaacacttcc
     5521 ggatgattca acagccacag aacatactcc ttgtccttca attcatgaag gatgtaagtc
     5581 ctatccttcc ccaccaacgc aaagaaatgg gccatatagc aagcctgcgc cagctcccga
     5641 tccttcaaca gggattccac ggaaaacgtt ttttccgcct ccaacttctt ttgtacatat
     5701 tggcgacaca gtccggcaat actccgtaca tcccctccct ccatctgcca tgccagacgc
     5761 tgataggata aattttcagc agcaaccact gataaaaaat acagagaaaa gaacagaaat
     5821 ttaaacacag aaacgttaaa aataacaatt atgcatctca atttaaaata tggaacattt
     5881 aatttattat attctattta taattaatca gttaatttat attttaataa atattctata
     5941 ggcaaaaaac ttctaaaatt tactcatcaa tcctatctca tatacacaaa aataaaaatc
     6001 aagcattggc gcaacaaata cttctctaaa ataatttctt actattaaaa ttcatttttg
     6061 cacactaatc ataccaggag cgatttcttt caaattaatc tcatgggtct ttctgtattc
     6121 attaatatga gccgatacat caaacatcaa aactctagta tcatcctctt tttccaaccg
     6181 gactcgatca ataaactttt tcccaaatac ccatgttgct cttgaaatta cttgataacc
     6241 aggaaaataa ttcttctgtg cttccaaaac ttccttctca gttttagcat ctttcaattc
     6301 aatgggaaaa tcataagaac ttcctggttc cttatcttct tttacacgtt ctttttgttt
     6361 ccggagatct ctagaaattt taataatttc ttttttatga agctcgttaa atttctttcg
     6421 ataatccgta atatcaaaac acacttctat tacatttccc tgttcgtttt ttaaatacac
     6481 agtttcaatg attgatttct tactggaaag atccacttcc ctgtcaccta tattataacc
     6541 aggataattt gtttccacat aattttcaat ccagctatcc agttctccac ggttttttac
     6601 accttctaca acgacagggg aagaagagtc attcttttcc aaattgactt tgctatccct
     6661 aggaacttta ttcatcatat ccttttcatg tgcataaaga aaccctgtag aaagtaaaat
     6721 gaggtttatt aatataattt ttttcataat attttatcaa tgatgttttt cacattcttc
     6781 tttaagttcc ttttcaaatt tcttaagaag atctcttttt gctgtagaca cggcactttg
     6841 agcacttttt tcccttcaat attcggatca aaaaaatcct tcatcccatc agctaaatgt
     6901 gttttcatcc ccgttttcca tggagtttta aatttatgat tatgaacaaa tctgtaggca
     6961 taccatagat gaacatgata agtatgtttc atggaggccc ctactagatg atccatatcc
     7021 actatccctg tttctgcgcc tagagcttca gtaagaatag attccaccat atcacccaat
     7081 gcctcctcaa caatgtcagg cggagtagga ggctctttac cccaaggagt aaagaaggca
     7141 agatatcttc tttctacacg gcatcttggc tgatcagcag ccaagacatt tccctgaaaa
     7201 aacagtaata tagagattat caaaataaac acacgcctca taacttactt cctaatctat
     7261 tgaaaaaaaa gaaaacatca atatctattt caacatgaaa tcaaattaat attttttggc
     7321 tcattcaaat acataaaaaa cggcgtcctt ccgtgataag gaggacgccg gaattttccg
     7381 gaaatcggac agactgccag tttgagcagc gaagcgcaca aaccttagga cttgtaacgc
     7441 aaacgcttct tgtgacggtt ctgacgcatg cgcttgctgc gcttatgctt gttgattttt
     7501 gctttacggc gttttttgag cgatcccatg attgtggttc gtttgttgtt ttactgtggt
     7561 gagaaaaaaa tctagtaaac cagcttgtta agcgggtatt caatgatgcc ttccgctccc
     7621 agggccttga gctggggaat gatatccctc acaacggatt cgtcaatgac cgtttccacg
     7681 gccagccagc cttcttccgc caaatgggaa atagtgggac ggcgcagaga cggaagggcc
     7741 tctaccaggc cgttcaggga agcagcagga aggttcattt tcaggcccac tttccggcgc
     7801 gcttccaaag cgcccttgag catcatgacc aggcgttcca gcttctgttt cttccactcg
     7861 tcctgcatgg tttcccggtt cgcgataaac tggggatagg aggtcatcag ggtgtccacg
     7921 atgcggagct tgttggcctt gatggagctt cccgtttccg taatgtccac gatggcgtcc
     7981 accagttccg gcaccttcac ttccgtagct ccccaggaga attccacgtg ggcttcaata
     8041 cccttttcct gaagccagcg ttccgtgatg cccacgcctt ccgtggcgat gcgcttgccc
     8101 tgaaggtctt ccgcacaggt aatgggggaa ttttccggga ctaccagcac ccagcgggta
     8161 ggcttggagg tagccttgga gtattgaaga tccgtcagca cttccacgtc cgccctgttt
     8221 tcataaatcc agtcgcgccc ggtaatgccg cagtcgatga aaccgtcatt gacataacgg
     8281 cctatttcct gggcacgaat caggaagagc tccagatcgt cgtcgtcgca ggtcgggcgg
     8341 tacccacggc tggacacata aacattgtag ccggccttgc ggaacaattc cacggtggaa
     8401 tcctgcaaac tgcctttggg aagagctatt ttgagtttct tcgacatgat gggacgggaa
     8461 tgaaaaccca cccgggcctc attgtcaagt ttagaaccga atccggccca ttttaccggg
     8521 ccatgagccg ttcaatgtca tcaggcagca gaggcacgtc tcccagcagg tcgatatttt
     8581 ctgtttcccc gatcaggaca tcgttttcca aacggatgcc tatgccttct tccgcaatat
     8641 agatgcccgg ttccacggtg aaaaccatac ccggcaacac aacggggttg gcttcgccta
     8701 catcgtgcac atccagcccc aggaagtggg aacacccgtg catgtaatat ttccggacaa
     8761 tgggaggatc ggacggtttt tccgccacct gtgcgggtgt gatgagcccc agcttgacga
     8821 gttccccggc agcaaaaacg cgcaccaggc gttcgtactc cgatttcagg attccgggtc
     8881 tcagaatctt tttggcgtag gtcatcatat tcagcacgct ttcatacaca gcgcgctggc
     8941 ggggggtgaa ttttccgttc acgggaacgg tgcgggtcat gtcggagttg taattcccgt
     9001 attccgtgcc tatatccata agcaccagat cgccgtcttc gcaccgtttg tcgttttgga
     9061 tataatgcag cacgcaggta tcctttccgg aagcgatgat gggcaggaag gagaatttgc
     9121 gcggaccgcg gctgatgaat tcgttggcca ggaatccctc aatctgccat tcgcccaccc
     9181 ccggcctgat gaaccggagc aattcccgga agccttcatt ggtgatgtcg caggcttttt
     9241 tgagagcctt gatttcttcc ggctttttga tttgccgcat atccgccatg atttcataga
     9301 cattcttcaa aacagcgtcc gggaattttt ccttcagctc cttggtcatg cgggcattgc
     9361 gcgtttccac cgggcatgtg catcgcggat gctggttggc ttccacaaag accatggatg
     9421 cggacggcac cagggcctcc agcaacgcat catattcatc ggtccagcgc acgtcctgga
     9481 tgccgctcag ctctctcgcc tgttcctgcg agagccgggc accttcccag atggcaatct
     9541 gttcatttgt ctcacgcaac agcagaatct catcccagcc gccctcccgg atagtcatga
     9601 ccaggacggt ttcttcctgg tcaactcccg taagataaaa gaggttggca ttctgatgca
     9661 gggcaaaagt gccgtccgca ttcgtaggaa atacgtcgtt ggcgtgcaga atcagcatac
     9721 tgccggcagg caggcgggaa gcaagttctt cacggttgcc ggcaaaaaag gaagaaggaa
     9781 gcggctcgta tctcatatca acagcatgcc ccgcttttcc ggcttgtcaa tcatgcatcc
     9841 ggacacgggg caaccttgcg cgtctccttg tcgtccggag ttcttctgac cgtgccgttt
     9901 ccccgctccg gcctattctt atgcgattgt ccatttcctg ccgtcccgct acccgtccgt
     9961 tcatcatgca taccgttgaa ggaagcacgg aaccgccccg gaatttccgg agacaccccc
    10021 tccggaaaaa actcccggag ccaatcgggt catgactcga acaccacccg ggcggatgcc
    10081 gtcttatcct gcatgcacgg cgctattcca cggctattcc tggcgctggg aacaggtttg
    10141 gttttttcct gttccagttc ccggacaatg cccgtttcct ctttgattac ccatcccctt
    10201 cacacggact ccaggcagag ttgttttgga ggcgtctggt ataatccctc ggaacaggcc
    10261 gtctggaatt ccccaaggct aaaagtatat gtagcccccg tcaatattga ctacatcaaa
    10321 gccagattcc ccaaggaatc cccggcgctg gcggcacaat tcagggccgg gctgcaaaaa
    10381 gacatccgca acgtgctgga aaacaaatcc aaacagacag gcgggaggat gaaatggcag
    10441 ttggttgacc gccccgttcc gggatccgta actctccgcc tcgccatagt caagctgaag
    10501 cctacggacg tgggcggcaa catcatgtcc gatcttgttt ccctggtctc cccccttccg
    10561 ggaacctccc tgatcctggg gcgcttcatg agcggggacg taggcattga gggacgcctg
    10621 tcaaatacga atacggacgc cggcatcatg gaattcaaag cttacaacac ggaccccatc
    10681 accctgtttt ccgtcaagga atttgaacgt tttgccttcg accaacggaa tttgcggctt
    10741 ttttccagct gcattgcaga tattttcaaa gctggtcccg ccagcagtat tcccaaaaca
    10801 ggggaatttg acctggaccc gttttaaacg gcctttccgg acgattcttt cccgtctttc
    10861 ttttcaggtt catagaaagc gcattcctga accgtctgcc tggtcttgaa ggcatataca
    10921 aaaaaagcgg cgcccaccag cacgaggaaa acggagagga actgcccgcg ggtcaatccc
    10981 atggagaagg gggaatccgg ctcccggtac tcttcgcaaa taattctgcc cgcagcataa
    11041 agaacggcaa aaataccgca gaacaaaccg ttccacgcac gaggaaacct tacccgtaca
    11101 aaccacagca cggcaaagag aagcacacct tccgcaaacg cttcatacag ctgggagggg
    11161 tagcgtgcat gatcctgcat catctgcccc acgatttccc tgatggcggg tgtatccctc
    11221 acccggtcgg tcacccaggc ggcagtcatt cgttccggca cagctatgcc ggaaagggac
    11281 agtttgtcca gcaatccggg cgtttcataa atacgggagg ccacccgcac gaacaagtcc
    11341 ggatcctggg aaagctccgc cggaaaaatc attccctggg aagagccggg cgggacaatg
    11401 cgcccataca gttccccgtt gataaaattg gccatgcgcc cgaaaaacag cccaacggga
    11461 gctacaatgg ccaggccgtc cagaagcgcc acccacttga cgcggtgctt ccacgcataa
    11521 taaaatgtgt acagccccac tccaatcatt cccccgtggc tggccatgcc cccttcccac
    11581 accctcagta ccatgagcgg atccgccaga aactgggacc agccgtgatt gggaatctgg
    11641 taaaagagca catatcccag ccttccaccg atcagaacac caaaaatagc cacataagta
    11701 acaaaatccg ccattctgtc ctgagggaca ggatacagct tccggcggga aagccaggaa
    11761 agcagataat acccgccgat gaagcccatt aaataggcca ggccatacca gcgcagggca
    11821 attgagtccg ttatctgcca gatgacaggg tccagatcat gtacgtaaac ggtcggattc
    11881 atgagcgttt ctttcagggc aggtaatggg taagggcttc cacggcctcc tggtccaggg
    11941 aacgggccag agccaccgtc aattccaccg cggcgcggta gtcccgcaga tccagcacgc
    12001 cgttatgggc atgaatgtac cgggtgggaa ttcccagcac aatagtggga actccctttc
    12061 cggagagatg cagggctgcc gcgtccgttc cgccggaacg gcgcaccgtc aactggaatg
    12121 ggatgcccgc atccaaagcc gttttttcgg ccagggcggc cagccgggga ttggtgatgg
    12181 ccgtggggtc aaacagcctg atttgcacgc ctccacccaa ggccccttgg gaatccgtca
    12241 cggcaaaccc gggggtgtcg tctgccggcg gcccttccag aacaatcacg caatccggtt
    12301 gcatggcgac gccggccgtc ctggcgcccc gcgtgcctac ctcctcctga accgtcgcgg
    12361 cggcaatcag ggtgttggga tgcccttccc ggcataattt ctcgaatgcc tctatcatca
    12421 cagaaagacc ggcgcggttg tcaaaggctt tccccatgac ccggaacgga ttttccaacg
    12481 gggagaattt cacgtccggc accacgggat cccccaggtg aataccgaat tcattcttca
    12541 cctgttccgc gctttcagcg cccacgtcca caaacagggc ttccatgctc atgacgctgt
    12601 tgcgctggcc ttccggcaga aaatggggag gcttggaacc aatcacaccc cggatgccgc
    12661 ggcccgagcg ggtttttacc aggacacgct ggcttaacag ggtatgtggc caccagccgc
    12721 ctatccccac caactgaagg aaaccgttcg acgctatatt ctgcaccagg aaacctattt
    12781 catccatgtg ggcggccagc atcacgcggg ggccggcccc gccgccggtg caaaacaggc
    12841 tgccggaacc atcggaagaa aattctccat atccttcaag ttcctgagcc accaggcttc
    12901 gcacttcatc ctcatgcccc gaaataccgt gggcctctga aaactttttc agcagatcag
    12961 tatcaatcgc catgggccga gcatgtggaa acacctctgc caggtcaagc ccgcagccat
    13021 gccgcatgtt tttttcttga ccgcccgggt agaaccgccg gacggagccg tgtcatgccc
    13081 acactcgcta catggaaaga aacaagagat ttttgctagc cacagttcgg tttttggtgt
    13141 aactaatcag ggaatcgaat tgtgctatga aatccatcct taacactatc acggccatgc
    13201 tggctgcggt gcttttcgtc cctgcggcat cggcgcagac caccagcaat cccagaatgc
    13261 aggtgcgggt ctccctggaa aagctgtccc tgtatatgcg ccagtccccc aacgtcctga
    13321 cgcaggacga tccccggccg ctgccgaaac cgaagaaatg ggcggatttt gaaattccct
    13381 tcaaggtgga agccgctccc acccccaaat ccggctatat tgatgccctg acgttcaaat
    13441 tttacatcgc ggtagttaat ccggaccgcg cccgccagta cctgaaactg tataaggagg
    13501 tcaaatacgt caatgttccg gtgggagaaa acacgtacgc ttccgtgtat ctctccccgt
    13561 cctccgtcaa gcgcattacc ggtgtggaag gaggaagagg aaaatgggtg aagtaccagg
    13621 gcgtagtggt ggaatacaac ggcaagattg tcgccactta ttcctccgaa cgcggcaaga
    13681 tggaaaaatg gtggaccatc cagtccccca gtatcgtgga gacctcttat taccccctgc
    13741 tgaacaagga tgaaacgcct ttctccgtgt tctggtacga ccgttatccg gaaattatga
    13801 ggcccaacag ccagcaggcg gcttccggtt ccgtccccgc cccgttcggt actcctgtga
    13861 tccccccggc ggacggcgaa taacgttcca ttaatgctat catttcatct tatttaacag
    13921 aaatcaccca tggctaattc acgaccaatc gtcgcattaa acgtaggttc ccagagagtt
    13981 tcaatgggcg tcttttccaa gacttccaaa gacgccctta tcctggaccg ctatgctacg
    14041 cgcctcgtag tgctggaccc gtcagcagaa ggcttgcgcc tgacgaaaat aggggaagcc
    14101 atcgccgacc tcgtccagga actcaacgtc aaaggaagcg tcgtcaacta ttccgtttcc
    14161 ggacaatccg ttttcatccg cttcgtcaag cttccggctc tggacgatac cgatgtggaa
    14221 cagctcatcc gctttgaagc ccagcagcac gtccctttcc cgctggacga agtcgtgtgg
    14281 gactaccatc tgcttcccgc caaaggtctg gaacgggaag ccatcctggt tgccatcaag
    14341 gcggaagacc tggactccct gaatgatgaa atcgtctccc acggcctttc caccggcaaa
    14401 gtggactgcg cgcttacctc tctgtacaat gcgtatgtgg acagttatcc ggatgagaaa
    14461 gaaccggtca tgctcatcga catcggtgcc aaatccacgg atttgattta cagcgaacag
    14521 ggacgtttct tcacccgcag catttccgcg gggggaatct tcgtaacctc cgctatcgcc
    14581 cgtgaattca atatttcctt catggaagcg gaacgcttga aaaccaccag cgggctcgtt
    14641 tccatgagca acgggcaaac ggaaggactg gatcccgcca cggccaatct ggctaccgtc
    14701 atccgtacgg ccatgaccag gctcgcctca gaaatccagc ggaccaccaa ccattaccgc
    14761 gcccagatga gcggaagcgc tcccgtcaag gcatacctgt gcggcggcgg agcttccctg
    14821 ccctatacca aggaattcct ggaagataaa ctgggcattc cgatctcctt cttcaacccc
    14881 atgcacaacg taggcgtggg ttccggcgtg gacgtgaaca ccatctcccg cgaagccttc
    14941 atcctgggcg gattgatcgg caccgccatt cactccatcg gcagggcctc cctcaatatt
    15001 gacctggaac ccaccgcagt tgccaaaaag cgggccaacc agaaaaaaat gcccgccatc
    15061 atcgtcgggg cagccattgc cgttcttggt gccgcagctt atgccgtcac aggatatatg
    15121 ggagagaaaa aggcggagga aatcctcgcc ggcgtccagc ccaccgttac ttccatcaaa
    15181 tcggcacaat ccgccctgcg ccagaaagaa caggagctga aaaaactgga ctccaccctg
    15241 gcttcctacc agcagctcac gcttcagcgc tacggctacg cggacatcat caggcacctg
    15301 ctggaacagt cggaacacaa ggattatccc tactggttta cggattttga accgctggcc
    15361 cacttcaatc cggaggatac cacccaaatc acgggatact ccgtcatcaa ggattccttc
    15421 acatcggaca aaaatacatc cttggtggat gacatcagga cggaggcctc cgccaacgca
    15481 tccagcgagg atgaagcggg cgtttacagc gtcaacgcca tccgcctgac cggattcgtc
    15541 cggcgcagcc tgggcggcca gagaatcatc caggacctcc aggcaaaaat agacgggaac
    15601 aaggaatccc tgttcacttt caagcccgga gacgtcaaac tggaagcccg ccagatcatg
    15661 gaactgggag ccaaggacgc caaggtggac gcggcggcag gggcctttgt acccttcaag
    15721 ctggtgcttc cgttaaaaac gcccattccc gtgaacttta acaaataaga ttacccaacc
    15781 cgaacaagac atattttatg agcaattgga ttacagacaa caagcccgcc gccatggtcg
    15841 cgggcgtggg gcttctctta ttcctggggt tatccgcgac agggtacatc gtcaattcca
    15901 aacgcagtga actggacaaa aaaatcagca tcgccgccaa ggaaatcaag tccgccaatg
    15961 ctgcggaaat cactccgagc cgctcatcca acgaagagct ggaaaaagaa ctgaaccgct
    16021 atgccaaggc cgtgggcagc ctggaaacgg cctacaagcc cttccttgcc tcctccgcgc
    16081 tggtccccac cacgcccacg gcattccaga atgaactgaa aacattcagg gattccctga
    16141 tctcctcctg caagaaaaag aacattctca taacggacac atcctcctgg ctcggcttcc
    16201 aggtttacag cacccaggct ccctctgttc aggcggcctc cacgctgggt tttgaattga
    16261 aagccatcaa cagcctggtc aacaaactgg cggaatgcgg cctgtccaaa ttcatcaagg
    16321 tgtaccgccc ccagctcccc attgaaaccc cggcgaacaa tccggaagaa tcggacgaag
    16381 ccgaccaggc tccatggact cccatgcctc tggaaatagc cttccagggc gaccgggaaa
    16441 gtgtattgaa agccatgaac gccataaccg gcatgcagga ttatctgttc acggtcaact
    16501 ccatccgtat ccgcaacgaa cggatgatgc cccctcccat cgccaatccg gcagccgcca
    16561 agcctgccgc tgcccaaccc gccacgggtg cggcttccct gactccggcg gatgaggcga
    16621 ccgcacctgc agccccggcc atccagcaag tcatcaagcc ttacatgggc aaggagcagg
    16681 tctttgtcca ggtctccctg aatctggtcc acttcaacca gcccaaagct caggaaccgt
    16741 ctgaagatta accaaggcaa caccaaccgc agcaaatact ttcaacatgt ctgaaaaaca
    16801 aaattacgac aaaatcctgt tggcatccgg aatcgtcctg ggcctgggag tggccgccta
    16861 tggtacgctg acctttctgg gactgaatga caaatacaaa ttcaccacgc aggtttctga
    16921 aaaagccatc gaaccgcctc ccggcatcaa gaaagccgca gaagtaggcc aggaactttc
    16981 cgcctcccac gaactcaagc cgattgccca ggaaacacag aagtatgtgg gcttcgtcgc
    17041 ccccaatctc tggattaagg aggggggaat ggaacccttt gacatcatct ccggtccgcc
    17101 tatccacggc aacattccga acaaatggtt cctggacaac ggactggaaa atgaattcgt
    17161 ttactcggac gtcctgaccc gggacccgga caacgatgga tttacggtgc aggaagaata
    17221 tgcggccaaa acacatccca acgaccccaa cagccatcct cccctcgtca gcaagctttt
    17281 tgtcgacgaa atcaagcagt tcggcttcta tctggctttc acccaagcgg atggcaatga
    17341 ctttaccttt aagggtatga accgcgccaa acaggaaatt tggaaaaaca tcgtccagac
    17401 caacggcaag ttcggcgccc gtaagaatac aaaagacgag cccaggttcg agcttgtcag
    17461 cgttgttaaa aaggaattca aaaatcccag cctggacatg gtggaaacgg acgaggaagc
    17521 cgtcgtcaag gacttgaagc cgaccaagaa cggacaaacc tacaccatca ggcgcggaac
    17581 aaaatacgtc attccaatca ttgataagaa agtaaacctt actatcaccg ccggccccga
    17641 acgggatacc agctttgaag tagaggaagg ttccgacttc cggattccgg gagacgccaa
    17701 acagatctac acgctgaaaa ccgttgataa tgctacacag accgtaacca ttgccaacaa
    17761 aaccactgga gaacaaacaa cactgagcaa gaaaaaatag acccggattt aaacttttct
    17821 gaaaaaaaag cttcacaaat ccgaaaaaca tgtaaaaaga agaacctaac tatggaccac
    17881 gcaccacttt atcaaccgaa gcgttccctg atcgcgctga tggccattgc tgcctcctgc
    17941 ccgtttgcgc aggctggtga tggcggcgcc gtcggaacct ccagtgcctt gggatcgtcc
    18001 tacgccggac cgggcagtta ccaataccag tccagtgcag cgcgcacagc catggcccgc
    18061 cgcgaagcgc aaacccagga agccatgcag ctccttgcgg aaggccgcaa cctgtaccgc
    18121 gaaggcaagt acaaggaagc cctggacaaa tacaacgccg cttacaacat gctgccttcc
    18181 gcgccgatca acgaccagcg caaggaagct atcgccaacc atattggcga cgccagcatc
    18241 gccgttgctc aggaatacat caaagtgggg cgctatgacg aagccgacaa gcttctgcag
    18301 gatgccatca agctcaatcc ccggagcgcc aagcttgcca agcagacgct cgaatacatg
    18361 aaggacccga ttcgcacgaa tccggcgctc acccccgaac acgtgaaaaa cgtggaaaag
    18421 gtgaacaccc tccttcacat ggcctatggt tactatgacc tcggcgacta cgacaaagcc
    18481 atcgccgaat tcaacaaggt tctctccatc gacccgtaca acgtggcggc ccgccgcgga
    18541 caggaaacgg tcaaccgccg cagaatggct tactatgcag ccgcttacga cgaaacccgc
    18601 agcaccatgc tggcggaagt ggacaagatg tgggaacgcc ccatcccgat ggaagtcccg
    18661 accggagccg acggcactga caacgcaccg atcacggaca tcaacggcgc cacggccaat
    18721 ctgatgaagc tcaagagcat catcatcccc tccgtctctt ttgaagacac caccgtggaa
    18781 gacgccattg actatctgcg caaaaagtcc attgaactgg accgcacggt aggcccgaac
    18841 ggtgaacgcg gcatcaactt tgtcatcaat gattcccagc ccgctgccgt cgcccccgcc
    18901 gttcccgcaa ctgatgaaga cggctttggc gaagaaactg cggaagtcac ggaagctgct
    18961 ccggcagcag ccccgcagga aagcatccgc acccgcaaaa tcggccagtt gaagctgact
    19021 aatgttccca tgctggaagt gctccgtttc atctgcagca atgccggcct gcgccagaag
    19081 gtggaagact atgcagtgac catccttcct gccggcggta atgacgtgga tctgtaccag
    19141 cgcaccttct ccgtgccccc gggcttccag tccgctctcc gcaccaccgt cggcgacggc
    19201 ggcggcgaag tcagtgacga cccctttggc ggcggtggcg aaagctcctc cggccttaaa
    19261 cccatgccct ccatccgcag cctgctgcaa aagagcggca tcagcttccc ggaaggcgcc
    19321 acggcattcc ttgtcaacgg caattcctcc ttggtcgtcc gcaacacttc cggcaacctg
    19381 gacctgatcg aacagctcat tgaaaacacc cgcggcgaat cccagcaggt gcgcatcatg
    19441 accaagttcg tggaagtaac ccaggaaaac acggaagaac tcggctttga ctggattgtc
    19501 accccgttct ccgtaagcaa tgaccgcagc accttcctgg gcggcggcac gaactacggc
    19561 accggttcca cttccgacag ctttacccag tctcccggcg gcgtgaccgg ctggcctgta
    19621 aacagcggca gcgacaccat caacggcctc gttaccggcg gcaaccgcac gggtgactac
    19681 gccatcacca agaactccgt ggacaatctg ctgaacagca ccaaccgctc tgaagcctcc
    19741 cagaaaaacg ccgctcccgg catcatgtcc ctgacgggca tttatgacga aggctccttc
    19801 cagatgctga tgcgcggcct gtcccagaaa aaagggtctg acgttctcac tgcccccagc
    19861 gtgaccgcca agtccggtga aaccgccaag attgaaatca tccgcgaatt ctggtatccc
    19921 accgaatacg aaccgccgga actccccaac tccgtaggca gcggctatag caatggttac
    19981 ggttatggga acaacggtaa cactctgaac ggtctgttgg gcaatcagat ccaaccccag
    20041 atatccagct tccctgtcac tcccgccact cccggcgtgt ttgaaatgaa gcccgtcggc
    20101 gtaactctgg aagtggtgcc taccattgga gacaacaagt acatcatcga cctgaacttc
    20161 aagcccagca tcgtggaatt tgaaggcttc gtgaactacg gcagcccgat ccagtccacc
    20221 ggcgttggtt ccgacggcaa gccgatgtcc ctgacgctga cggaaaaccg catcgagcag
    20281 ccgatcttct ccaagaggtc cgttgaaacg tccctgttca tctacgacgg ccataccgtg
    20341 gcaatcggtg gtttgatcac ggaaaacgtg cagacggtgg aagacaaagt gccgatcttc
    20401 ggtgacctgc ctctcatcgg ccgcttcttc cgcagcaact ccgacaacca catcaagaag
    20461 aatctgatga tcttcgtaac gggacagatc attgacgcca cgggccagcc cgtacgcggc
    20521 aatgcccttc ccactgcggc cgctccggaa agcgcccttc ccgcctccga aggcctgctg
    20581 cctcccatgt aggctgcgtg cataacgtcc atattggagc gggtgaaagg gtaacccttt
    20641 cacccgctct ttatatccca gaagcgcctt actttccctt ccccccatga aaaccctgat
    20701 tgttaccgga ggaatagcca ccgggaaatc tacattcatc cgtctgctga tggaggcggg
    20761 aggcgcgcgc ctgcgcctgt ttgactgtga tgcggaggcg ggcagattgc tggatggcgg
    20821 tacgctgaaa gcccccctct ccagcgtctt cggcccagcc agcgtagact cctccggaaa
    20881 ggctgacagg cattttctgc gggagcttgt tttccggaat ccggaaagcc gcagaacgct
    20941 ggaagggatc attcaccccc tgctgcatca agaatgtctt gcgcaaatgc tggcggcgcg
    21001 tcagaatacg gaggtggacg ggtttctcat tgacgtgccc ctgttttttg aaacttcggc
    21061 gcgctattgc caagatgccg tgtgcgtggt ggctgtttcc aggggaacgc agaaaacccg
    21121 cctggctatc aggaatggat tccgggagga tatgattgaa gccattctgg cggcccagcg
    21181 gcctatcatg gaaaaagtgg ctgcggccga cttcgtcatc tgg
//
LOCUS       DAOMSU010000007        19837 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_70_length_19837_cov_3.342180, whole genome shotgun sequence.
ACCESSION   DAOMSU010000007 DAOMSU010000000
VERSION     DAOMSU010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 19837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19837
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70_length_19837_cov_3.342180"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PK077_00725"
     CDS             <1..929
                     /locus_tag="PK077_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22856.1"
                     /translation="QLIFFMNYYLSLCLSVFLFFSVGSRADEAAYGDLPSFMNIRPER
                     LSGDARHLKDLLDQGKLGDFYQQALPVLNRELFDEDGGMTPEELENALFINNMVVKAP
                     YQDYRLDNFSIWINGSELETKFKAYTNLGKIETYVRENKRLRQGKELARFCLEAEFEY
                     IRRMMLQGAAVYRYIAGLEAEHGKFHPVRPVDTLPYWRYRETKNLAACGAEEERRRKA
                     LYERNVDELVRLRQSFRWYIKHREGVLLNIVLKLHPGNRGRALERLRECGYKTDRELV
                     EFLERTGGMRKRVQFLYSPGMLREHRKLGKED"
     gene            935..1864
                     /locus_tag="PK077_00730"
     CDS             935..1864
                     /locus_tag="PK077_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22857.1"
                     /translation="MAANILSGMVPLSLSFFLLFSLGSRAEEAAVYDDLPSFMDIRIE
                     RLSGDARHLKDLLDQGKLGDFYQQALPVLNRELFDEDGGMTPEELENALFINNMVVKA
                     PYQDYRLDNFSIWINGSELETKFKAYTNLGKIETYVRENKRLRQGKELARFCLEAEFE
                     YIRRMMLQGAAVYRYIAGLEAEHGKFHPVRPVDTLPYWRYRETKNLAACGAEEERRRK
                     ALYERNVDELVRLRQSFRWYIKHREGVLLNIVLKLHPGNRGRALERLRECGYKTDREL
                     VEFLERTGGMRKRVQFLYSPGMLREHRKLGKED"
     gene            1870..3639
                     /locus_tag="PK077_00735"
     CDS             1870..3639
                     /locus_tag="PK077_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22858.1"
                     /translation="MAANILLRMLLCCVLLLVSGVSRARDATAVESLLRADYSSVIID
                     GVRNQLRKHLDSGTLSPEKIRRIVHHPELEQLCHIGQFFRYASQEKAFSTRELRDRTF
                     TRWLVSHPEVFRKLAHAGYAQQSSLAILYRLWNSADRDLCGVDLNIALGACLIADVFT
                     AEECIARYGFYHDSHHHGRCYPQADSLEPWEWAVVLRGKESLEDLAWAQQFIEGKNIK
                     PEQAGGKFTGFIPYRKKNHRGISIHAGAAFYDNKPITLQLYTEYGGVCGAVSKGAAGF
                     LRAKGVPAYPIGQPGHCAFIWKHPKGQWLIGNNIGGWNWANGGNKLPWKGPVQIISAL
                     CAFWQGPQARESSLMYDLSFYSRNPQHVELLLQEACRANPYNYPAWVRYLGIKAGGAG
                     DKKKLEYLIQFSKAMPREHNLIDYVAGHVLKINPEKVNPYELYACGVSPDSTPEAEEL
                     FIRQFWKKLIADCPEVKLHAVYKEGTTGNFLNLWLEKSKNIKWNSKMKSKTSKAFQQA
                     LLALTGKEKIFIKMMKSYRRFLIMWNDEKYMMQAHEFVQDHLKGITSDQVKKEMAGMG
                     RKMGILLKNDKILNMYGQNRESE"
     gene            complement(3665..6172)
                     /locus_tag="PK077_00740"
     CDS             complement(3665..6172)
                     /locus_tag="PK077_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3656 domain-containing protein"
                     /protein_id="HRN22859.1"
                     /translation="MTSDDMTLSALRPGDLRPELLAPAGDMDCARAAVANGADAIYFG
                     LDRFNARLRANNFTLDSLPELMRFLHAHGVKGYVTMNTLIFTSELADALSYLGHLNAA
                     GADGVIVQDIGLARCLTEWGRQVPGMKLELHASTQMTLSSPDGLEFASGFLDLKQAVL
                     ARELSLKEIGQCVRHTDIPLEVFVHGALCVAYSGQCLTSESLGQRSANRGECAQACRL
                     PYSLVVAGKAVPLGERRYLLSPQDLCAIDRIPDLVCMGVKSYKIEGRLKSPEYVAAVT
                     AAYRKALDAACAGIPVDEMVTVRDRYALQMVFSRGFSTGWLDGTDHPRLTHGRYGKKR
                     GAYAGVIMNSGQGWLDIRPEGGILLAPGDGFVIDAGEDRNEEQGGRIWKVQRNRLFFH
                     GKASRIDWSRVRPGQKLWKTDDPALNAELKKMREHLPEATVPLHLTCTGSAGEPLTVS
                     CPEYGCSVQSAQPLQTAEKRPLTPEALKQQLGRLGGTGFRLASCECRLPNGLMLPLSI
                     LNQARRALVEQIQTVRQEREAPVPSRLSAAFTPSALPAGAAAPDAPPHLSVLCRRPEQ
                     IPSVLDAGADAVYLDFEDLRDYAAGVKAVRQHADSIPVFLATPRIQKPSETGYFKLME
                     RAEPDGILIRNLGAAQYFRHSPLRRIGDFSLNVANPYSAAILKERGRLEYLTLSYDLN
                     AEQVADLLRAAPPEWFELTLHQHMPMFHMEHCVFCTFLSDGTSYKNCGRPCERHHVQL
                     RDRVGQLHPLLADAGCRNTLFNGRAQTGAGFLRGFRRLGLSRFRLELLDDPPEKIRLL
                     VSRYRELLDGSCTAARLIRELDVAEQLGTTEGTLRPR"
     gene            complement(6199..7413)
                     /locus_tag="PK077_00745"
     CDS             complement(6199..7413)
                     /locus_tag="PK077_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HRN22860.1"
                     /translation="MNVSAALDWLFSTQFFGIKLGLDNTEKLLAAAGADRINATVVHV
                     AGTNGKGSTCAMIEALARAQGYVTGLFTSPHLVDFSERIRVNGNMITPDALAEEISFL
                     KQLAEGWEQPPTFFELALAVALRHFRKNSVNFIILETGLGGRLDATNAVPKDIAVLAP
                     IGLDHQQYLGDTLEEIAAEKAAIIAPGKPSVTAVQHPDVMAVMERTARNRRSPLTIAR
                     ADRNTPRPCLPGAHQRENAALALETMRRLHALPSPAAAAAALARVQWPGRFERLETPP
                     LVLDGAHNEHAARVLAATWKEEFPGRKAALVFAASADKHIREMIPVLREIAGEWHLVP
                     CTSPRIMPAEEMAALLAGQETGPVFIHSTLPDGLQAALASPFPSLAAGSLFLLGDLKA
                     LLRHAEKRSTAQ"
     gene            complement(7479..7573)
                     /gene="ffs"
                     /locus_tag="PK077_00750"
     ncRNA           complement(7479..7573)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK077_00750"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(7625..8641)
                     /locus_tag="PK077_00755"
     CDS             complement(7625..8641)
                     /locus_tag="PK077_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22861.1"
                     /translation="MRYLLLILLLAGGYYGWDYYQNGLAPRHEALKAMEQEPCPELDA
                     AQARFDEAAELHEQQAARLQKAIQRKEEAVRKYWDARIKQAGQEKDGKKTPSPVSGKP
                     NARSAEARIAHLLEQYDRRVAEVEELKSRRDATRQQLDSARNRLQEQIRQVETRQDIN
                     RIQLAEASNTKNRNFKVTENRTDLLKLQAGLPQELERITDQGEAALRDQTEAYEKAKR
                     ELGLFQNNVDRQIASLRDSMNSPLAEDYDETEILSEDPEFRTMIAPYDQAVSHEETIV
                     MRTEADVEEQAKALHGLQRVRDAKIEEERSRLRQEEEIFFTAATVIGVILLISILLSF
                     ARRR"
     gene            8808..10229
                     /locus_tag="PK077_00760"
     CDS             8808..10229
                     /locus_tag="PK077_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRN22862.1"
                     /translation="MSIFSSRRQFLKSLGLAAGAAAAGNALPGKAVEIPAGDHLWESA
                     SPAAPRPSGSTYMGGFKAPRLGRIRLAFIGVGGRGFSHLAQMCVMDGVEIVGICDLKE
                     ELTKRGVDRVLSRMGKSPLGYSGGDMEYLTMLKELKPDAVIISTDWSSHARIACDSMK
                     HGAHAFVEVPLAVSLEDLWSLVDTSEATRKHCMMMENVNYGRDELMFLNMVRQGVIGD
                     LLHGEAAYIHCLVTQLGDTRGEGAWRPEYHTRINGNLYPTHGLGPVAQYMSLERGEDR
                     FCRVAAFASPALGRNAYAKKHLPADHRWNNTPFICGDMNTAVVKTQLGRTILVQLDET
                     SPRPYSRANLIQGTEGTLAGFPTRVAGEKLGNGNYHEWIEGREKLAAIYEKYDHPLWK
                     RIGELATKMGGHGGMDFVMLSRIVECLRNGEPMDQNVYEGASWSSLLPLTARSIAQGG
                     MPVEFPDFTRGDWKTTMPLAVVS"
     gene            complement(10290..12977)
                     /locus_tag="PK077_00765"
     CDS             complement(10290..12977)
                     /locus_tag="PK077_00765"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRN22863.1"
                     /translation="MQPAPYHAVPEEKRNINGFRILPSGALVTDTPPFDRLFEEGTGN
                     GLYALAVSNWETVPDPGAFFIKNMARQALTRMAHAAAERPDEVDAVLDHLLASATEKA
                     FLAEQFPPVPGAEYATPDIIAGWFEDLRKAIAKEIRARGITPAEWLNSLGAPWNQIGK
                     VFFHLAENRDDATGSRPFAFMATFAHQSAADNQVRHLPLATALKLHEGNYTALLAVLQ
                     PLKEAARESPLLKRLLDNGKIYTPMAWSAPEARDFLQDTPLYERAGIIVRMVNLWKNP
                     PPKLQVKVTADASGSEKTVHSSGLSVRSLLKFSVSATLGGKDLTPEELGELLSNGDGL
                     IRFKGEWVRVDARKVRDLMDRWTQAARMMSSLGIPLVQGLRMLVNGPSVQLVQIPEPD
                     EDCVMEPGQNLRQTLDILAGKVPVSIPELPSRLDALMRPYQKEGFSFLYRITERGFGA
                     CLADDMGLGKTLQAIAWLDALRRKGRLEGLPALIVAPASLLSNWKEEAQRFAPELILR
                     IMHPSSPDPWQPENTLRRKDCHAVITTYGMAARTSALADLHFPAIILDEAQAIKNAGS
                     ARSRAIRSLHGERRIALSGTPVENNLNELWSLMEFLNPGLLGSRKSFESFTRSLERGY
                     APLRRLVRPFILRRMKTDPALVPDLPDKMEIPAYCSLTPEQAALYQAQIDMLHAVLDE
                     PDPAARLMLILPILARLKQICNHPAQFQGTDDYAPERSGKFRRLQELCASIAARQEKT
                     ILFTQFRSIIPHLHDLLSGVFGRSGLTLHGGTPIPERQNIVTAFQKESGPPFCILSLK
                     AAGTGLTLTQASHVIHVDRWWNPAVENQATDRAYRIGQHRNVLVHRLICRGTIEDRID
                     AMLKDKRRMADDLFSGGPEQWLMNMSAEELNNLFSGSPC"
     gene            complement(13015..14472)
                     /locus_tag="PK077_00770"
     CDS             complement(13015..14472)
                     /locus_tag="PK077_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HRN22864.1"
                     /translation="MIPLRIVLASLACLCPFSLAQEPKSDPDKTLIIISTSDIHGNLD
                     NFPRLATLVKQYRAKYPHVLLVDSGDYFIGNPYVDDCEKRGEPLTILMNKLGYDVVTI
                     GNHDLDYGQEALRDHIKGMPSTKFVITNANLSPTLENCFSPYVSIPIEGTSVSVGFIG
                     LADLQTTDVRKMAGISWTLPDEEDYKGITDRFRLHHNTINVILSHLGYGNDLKMMKYS
                     PNIDVILGGHTHVMLPSGHLRTGTLLSHTGHSLSHVGVTEIIFSTEHPVSILSKSTRA
                     VSLNEEIPNDPEVKEIVRRFSGNPLFNQQVGVAGEEITHVTIGTLFCKAIQQASHSDI
                     AIYNRGGVRSKNHLNKGPVTIRDIYELEPFREKIVTCSMSKADIEQLILSKFMSPTDD
                     EGGILEVYCSGFSYQIMDGVTPSITSTLKNGVIYTVAMGDYLCSNFIFPQRGNGKPTG
                     IDVRKALIDYLKQIKKLLNPPPSKLPIIRDTTFAL"
     gene            complement(14515..15018)
                     /locus_tag="PK077_00775"
     CDS             complement(14515..15018)
                     /locus_tag="PK077_00775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRN22865.1"
                     /translation="MKWFFPVLLFFLMPGCSTTEKCAQKTEPSVQEAEAHVKNAPYRV
                     RGKRYTPMSVADALQYHETGYASWYGGKARRLKTSSGEYINPQRSMTAAHKTLPMPCK
                     VKVTCLETGKSTVVRINNRGPFHSNRLIDLTTAAASRIGLHRRGVSRVRLEVISVGDG
                     PHEVSAR"
     gene            15168..16040
                     /locus_tag="PK077_00780"
     CDS             15168..16040
                     /locus_tag="PK077_00780"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRN22866.1"
                     /translation="MNSSKVIGLVALAEKPGLGEALRIMRRELSRHGLGSCVIETPDA
                     LERAIGRCGMLVTFGGDGTMLTVSSLAAMHRVPLAGVNLGRLGFMTTCSVQELPLLAY
                     ALQEGSYLTDERSMLEVVRAGEDGVAAPPRKLALNEVSLIRAQSGKMVDLDAEIDGEL
                     LNRYHADGVLVSTPTGSTAYSLSAGGPLVWPMSRVVCVTPICPHSLTNRSVVLPDNMT
                     IRLRPRERRGRFDSMVYSLDGRSAYPIEVGESLVIRKAPETLSLVHLRKQNFGALLRA
                     KLRWQGAEIPSDDE"
     gene            16264..16491
                     /locus_tag="PK077_00785"
     CDS             16264..16491
                     /locus_tag="PK077_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRN22867.1"
                     /translation="MKTHVKKGDEVEIIAGRNHKGKRGTVLAVNASKGTVIVEGARTL
                     KKAVRPTEQNPEGGIQEINGPIHISNVKKVG"
     gene            16558..17139
                     /gene="rplE"
                     /locus_tag="PK077_00790"
     CDS             16558..17139
                     /gene="rplE"
                     /locus_tag="PK077_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRN22868.1"
                     /translation="MSTPTLQTYYREKVVPGLQAKHAYKNVHQIPRLEKIVVTSCMGK
                     HPDRKQAVEDAVNEIAKITGQKPSVTYARKSVANFKVREGEPLGARVTLRGARMWEFL
                     DRFINITAPNIRDFRGLPYKSFDGNGNYAVGISDQSIFPEIELDQIKRQIGFDIIFVT
                     SAPTDDEGRDLLRALGVPMREPKKAEETQTANA"
     gene            17152..17553
                     /gene="rpsH"
                     /locus_tag="PK077_00795"
     CDS             17152..17553
                     /gene="rpsH"
                     /locus_tag="PK077_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRN22869.1"
                     /translation="MAVLSDPISDFLTRLKNASLAGNEEFTAPCSNIKVEIARILQEE
                     GYIWNYEVTGEGSKKQIKVKIKFTPQGKPVVTDVKRSSKPGRRQYVSSEDIPRVLSGL
                     GIAIVSTSRGVMTGAQARKQSIGGELLALVW"
     gene            17575..18114
                     /gene="rplF"
                     /locus_tag="PK077_00800"
     CDS             17575..18114
                     /gene="rplF"
                     /locus_tag="PK077_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017722933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRN22870.1"
                     /translation="MSRVGKKSITLPDKVTVKVNGSSVQVEGPKGKLSWTLPEGITAT
                     VEGNQLSVDRVGESRQLRALHGTNRSLLNNMVIGVSEGFVKNLEIVGVGFRAAVKGNI
                     LDLNLGKSHPINQVIPEGLKVTVTENTKVTVEGIDKQVVGQFAAEVRAYYPPEPYKGK
                     GVRFVGEVIRRKEGKSVGK"
     gene            18150..18512
                     /gene="rplR"
                     /locus_tag="PK077_00805"
     CDS             18150..18512
                     /gene="rplR"
                     /locus_tag="PK077_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRN22871.1"
                     /translation="MSTINRKAIRAKVRRRIRRKVSGTASRPRLAVYFSNRHVYAQII
                     DDTVGKTICSASTADASIADPSKLANCATATKVGNLIGERAKAANVTEVVFDRGGFKF
                     CGKVKALADAARETGLKF"
     gene            18706..19191
                     /gene="rpsE"
                     /locus_tag="PK077_00810"
     CDS             18706..19191
                     /gene="rpsE"
                     /locus_tag="PK077_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRN22872.1"
                     /translation="MEEGPQLAEKVVFINRCAKVVKGGRRFSFSALVVSGDMEGRVGF
                     GFGKANEVADAIRKATEAAKRQLRPVSIANGTIPHEVYSEFGGGKVLLKPASPGTGLI
                     AGNTIRAVLEAAGVRDIVAKSLGSSNHANVVKATLQALASLRTSDQVLADRGKKKEKA
                     I"
     gene            19254..19712
                     /gene="rplO"
                     /locus_tag="PK077_00815"
     CDS             19254..19712
                     /gene="rplO"
                     /locus_tag="PK077_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRN22873.1"
                     /translation="MLQLHTIKPNPGAKHRKKRLGNGESSGLGKTCGKGNKGQKARSG
                     GTIRPGFEGGQMPLHRRLPKKGFNNTRFQDKILIVNLSQLERVFEAGATVDENTLRAA
                     KLVQGPCDAVKLLGNGTLTKNLNVVVDFVSASAREKVTQAGGTVTVLSEA"
BASE COUNT         4197 a         5507 c         5940 g         4193 t
ORIGIN      
        1 agcaactgat tttctttatg aactattacc tttccctctg tctttccgtg tttcttttct
       61 tttccgtcgg gagccgcgcg gatgaagccg cgtacggcga ccttccttcc ttcatgaaca
      121 tccggcccga aaggctgagc ggggacgcgc ggcatctgaa agacctgctg gaccagggga
      181 agctgggcga cttttaccag caggcgctgc ccgtgctcaa cagggagctg tttgacgagg
      241 acggcggaat gaccccggag gagctggaaa acgccctgtt catcaacaac atggtcgtca
      301 aggcccccta tcaggactac cggctggaca acttttccat ctggatcaac ggaagtgagc
      361 tggaaaccaa atttaaggca tatacgaatc ttggaaagat tgaaacctat gtcagggaga
      421 acaagaggct caggcaaggg aaggaactcg cgaggttctg cctggaggcg gagttcgaat
      481 atatcaggcg gatgatgctt cagggcgctg ccgtttaccg ctacatcgcc gggctggagg
      541 cggagcacgg gaagttccat cccgtgaggc cggtcgacac gctgccctac tggcgttacc
      601 gggagacgaa aaacctcgcc gcctgcggtg cggaagagga acggcgcagg aaggccctgt
      661 acgagcggaa cgtggacgaa ctggtccgcc tccggcagtc cttccgctgg tatattaaac
      721 acagggaggg tgtcctgttg aatatcgttc tgaagcttca ccccgggaat aggggccgcg
      781 cgctggaaag gctgagggaa tgcggctaca agacggaccg ggagctggtg gagtttctgg
      841 agaggaccgg cggcatgaga aagagagttc aattcctgta ctctcccggg atgctccggg
      901 aacacagaaa actcgggaag gaggattagc cgccatggcc gcgaatatcc tgtcggggat
      961 ggttcccctc agcctttcct tcttcctgct cttttctctg gggagccgcg cggaagaagc
     1021 cgctgtgtac gacgatttgc cctccttcat ggacatccgg attgaaaggc tgagcgggga
     1081 cgcgcggcat ctgaaagacc tgctggacca ggggaagctg ggcgactttt accagcaggc
     1141 gctgcccgtg ctcaacaggg agctgtttga cgaggacggc ggaatgaccc cggaggagct
     1201 ggaaaacgcc ctgttcatca acaacatggt cgtcaaggcc ccctatcagg actaccggct
     1261 ggacaacttt tccatctgga tcaacggaag tgagctggaa accaaattta aggcatatac
     1321 gaatcttgga aagattgaaa cctatgtcag ggagaacaag aggctcaggc aagggaagga
     1381 actcgcgagg ttctgcctgg aggcggagtt cgaatatatc aggcggatga tgcttcaggg
     1441 cgctgccgtt taccgctaca tcgccgggct ggaggcggag cacgggaagt tccatcccgt
     1501 gaggccggtc gacacgctgc cctactggcg ttaccgggag acgaaaaacc tcgccgcctg
     1561 cggtgcggaa gaggaacggc gcaggaaggc cctgtacgag cggaacgtgg acgaactggt
     1621 ccgcctccgg cagtccttcc gctggtatat taaacacagg gagggtgtcc tgttgaatat
     1681 cgttctgaag cttcaccccg ggaatagggg ccgcgcgctg gaaaggctga gggaatgcgg
     1741 ctacaagacg gaccgggagc tggtggagtt tctggagagg accggcggca tgagaaagag
     1801 agttcaattc ctgtactctc ccgggatgct ccgggaacac agaaaactcg ggaaggagga
     1861 ttaaccgcca tggccgcgaa tattctgctg agaatgctgt tgtgctgcgt cctgttactg
     1921 gtatctggcg tttcccgggc ccgggatgcc accgccgtgg agagtttgct gagggcggat
     1981 tattcttcag tcatcattga cggcgtacgg aatcaactcc ggaaacatct tgattccggt
     2041 actctttcac cggaaaaaat ccggcggata gtacatcatc cggaattgga gcaactgtgc
     2101 catatcggac aatttttccg ttatgccagc caggaaaagg cgttttcaac aagagagctc
     2161 cgggaccgga cgtttaccag gtggcttgtc tcccatccgg aagttttccg gaaactggcc
     2221 catgcagggt atgctcagca gagcagtctg gctattcttt accgtctgtg gaatagcgca
     2281 gacagggact tgtgcggagt tgaccttaac atcgccctgg gggcctgtct catcgccgat
     2341 gttttcacgg cagaggaatg tatagccagg tatggatttt accatgattc ccatcatcat
     2401 ggcaggtgtt atccgcaggc cgacagcctt gaaccgtggg agtgggccgt ggttctgaga
     2461 ggaaaagaga gtctggaaga tctggcatgg gcccagcaat ttattgaagg gaaaaatatc
     2521 aaacccgagc aggcaggggg taaatttacc ggatttattc cctaccgtaa aaagaatcac
     2581 agaggaatca gcatccatgc cggggccgcg ttttacgata acaagcccat cactcttcag
     2641 ctttatacgg aatatggagg cgtgtgcgga gccgtttcca agggggccgc gggtttttta
     2701 agagccaaag gcgtcccggc gtaccccatc gggcagccgg ggcattgcgc ttttatttgg
     2761 aagcacccca aagggcaatg gctgatcggc aacaacattg gaggatggaa ctgggccaat
     2821 ggtggaaata aactgccgtg gaaaggcccc gtgcagatta tctccgccct ctgcgctttc
     2881 tggcagggtc cccaggcccg tgaatcttcc ttgatgtacg atttatcttt ttattcccgg
     2941 aatccgcagc atgtggaact gctgcttcag gaggcgtgcc gggccaatcc ttacaattat
     3001 ccggcctggg tgcgttattt gggcatcaag gcgggaggtg cgggcgataa gaagaagctg
     3061 gagtacctca tccagttcag caaggcgatg ccccgtgaac ataacctgat tgattacgtg
     3121 gcgggtcatg ttttgaagat taaccctgaa aaggtgaatc cctatgaatt gtacgcctgc
     3181 ggggtcagcc cggacagtac gcccgaagca gaggaacttt tcatccggca attttggaaa
     3241 aagctgattg ccgactgccc tgaggttaaa ctccatgctg tttacaagga agggactacc
     3301 ggtaatttct taaatttgtg gctggaaaaa tcaaaaaata tcaaatggaa ttccaaaatg
     3361 aagagcaaaa ccagcaaagc ttttcaacag gcgttgctgg ctttgaccgg caaggaaaaa
     3421 attttcatta aaatgatgaa aagctaccgc cggtttctca tcatgtggaa tgatgagaaa
     3481 tatatgatgc aggcgcatga atttgtgcag gaccatttaa aaggcattac cagcgaccag
     3541 gtgaaaaagg agatggccgg catgggtaga aaaatgggta tccttttaaa gaatgataaa
     3601 atattgaaca tgtatggtca aaaccgggaa tccgaatagg gattgaccat tgttcaaagg
     3661 gacattatct gggccggagc gtaccttccg tggtgccgag ctgttccgcg acatccagtt
     3721 cccggatgag ccgggccgcc gtgcaggagc cgtccagcaa ttcccggtag cgggacacca
     3781 gaaggcgtat tttttccggg ggatcatcca gcagttccag acggaagcgg gaaagaccca
     3841 ggcggcggaa gccccggagg aagccggctc ccgtctgagc gcgtccgttg aacagcgtgt
     3901 tccggcaccc ggcgtccgcc agcaggggat gaagctggcc tacgcggtca cgcagctgca
     3961 catgatgccg ttcgcagggg cgtccgcagt tcttataact ggtgccgtcc gacaggaagg
     4021 tgcagaacac gcaatgctcc atgtgaaaca tgggcatatg ctggtgcaga gtcagttcaa
     4081 accattccgg cggcgcggcg cggagcaggt ccgccacctg ttccgcgttc aggtcgtaag
     4141 agagggtgag atattccagc ctcccccgtt ctttcaggat agccgcgcta tacggattgg
     4201 cgacattcag ggaaaaatcc ccaatgcggc gcagggggga atggcggaag tattgcgccg
     4261 cgcccaggtt gcggatgagg atgccgtccg gctccgcccg ttccatgagc ttaaagtaac
     4321 cggtttcaga cggcttctgg atgcggggcg tcgccagaaa gacggggatg ctgtccgcgt
     4381 gctgccgaac cgctttcact cccgccgcat agtcccggag gtcttcaaag tccagataaa
     4441 cggcatccgc cccggcatcc agaacggaag gtatctgttc cggtctgcgg cacagcacag
     4501 acaggtgcgg cggcgcgtcc ggagctgccg cccccgccgg aagcgcggaa ggggtgaaag
     4561 ccgcggaaag acgggaggga acgggggctt ccctctcctg ccggaccgtt tgaatttgtt
     4621 ccacgagggc gcgccttgcc tggttgagga tgctgagcgg gagcatcaac ccgttcggca
     4681 gccggcattc gcaggaagcc aggcggaacc ccgttcctcc cagtctgcca agctgctgtt
     4741 tcagcgcttc aggagtcagc gggcgttttt ccgccgtttg caggggctgt gcggactgca
     4801 cggaacaccc gtattccggg caggaaaccg tcagaggctc cccggcggat ccggtgcatg
     4861 tcaaatggag gggtacggtg gcttccggca ggtgttcccg catttttttc aactccgcgt
     4921 tcagggccgg atcatccgtt ttccagagct tctgccccgg cctgaccctg ctccagtcaa
     4981 tgcgggatgc ttttccatga aagaaaaggc ggtttctctg cactttccag atacgaccgc
     5041 cctgttcttc attgcggtct tcccctgcat caatgacgaa gccgtcaccg ggcgcgagca
     5101 gaatgcctcc ttccggtctg atatccagcc agccctgacc gctgttcatg atgacgcctg
     5161 cgtaagcgcc cctcttttta ccgtagcggc cgtgggtcag acgcgggtgg tccgttccgt
     5221 ccagccagcc tgtggagaag ccgcgtgaaa ataccatttg cagtgcgtag cggtcgcgta
     5281 cggtcaccat ttcatccaca ggtattccag cgcaggcggc gtccagggct tttctatagg
     5341 ccgccgtcac cgccgcaacg tattccgggc ttttcaggcg accctctatc ttatagcttt
     5401 ttacgcccat gcagaccagg tccgggatgc ggtcaatggc gcacaggtcc tgggggctga
     5461 gcaggtagcg ccgttctccc aggggaacgg ctttgccggc tacaaccagg gaatagggca
     5521 ggcggcaggc ctgcgcgcat tcccccctgt tggcgctgcg ctggcccagg ctttccgatg
     5581 taaggcactg gccggagtag gccacgcaga gcgccccgtg aacaaatact tccaggggta
     5641 tgtccgtatg ccgtacgcat tgcccgattt ccttcaggct cagttcccgg gccaggacgg
     5701 cctgcttcag gtccaggaat ccggaggcga attccagccc gtccggagag gaaagcgtca
     5761 tctgggtgga ggcgtgcagt tccagcttca tgccgggaac ttgccttccc cattccgtca
     5821 gacagcgggc aagtccaatg tcctgcacga tgaccccgtc cgcgccggcg gcattcaggt
     5881 ggcccaggta ggagagggcg tccgccagct cggaggtgaa aatgagcgta ttcatggtca
     5941 cgtacccctt cacgccgtgc gcgtgaagga aacgcatcag ttccggaagg gaatccagcg
     6001 tgaagttgtt tgcgcgcaga cgggcgttga aacggtccag accgaaataa atggcgtctg
     6061 cgccattggc tacggcggcg cgggcgcagt ccatgtcccc ggcgggagcc agcagttcgg
     6121 gacgcaggtc tccgggccgg agggcggaaa gggtcatgtc gtcggaagtc acggggaaaa
     6181 gcaaagaggc tgtaggggtt attgggcggt actgcgtttt tccgcatggc ggagcaatgc
     6241 tttcaaatct cccagaagga acagggaacc tgccgccaga gaggggaacg gggaagccag
     6301 cgccgcctgc agcccgtccg gaagcgtgga atgaatgaaa acggggccgg tttcctgccc
     6361 ggccagcagg gcagccattt cttccgccgg catgatgcgg ggggaagtgc agggaaccag
     6421 gtgccattct ccggcaattt cccgcaaaac cggaatcatt tcccggatat gcttgtctgc
     6481 ggatgcggca aaaaccagcg ccgccttccg tcccgggaat tcctctttcc acgtggccgc
     6541 gagcacgcgc gccgcatgtt cgttatgagc gccgtccagt accagagggg gcgtctcaag
     6601 gcgttcgaac cgtccgggcc actgtaccct ggccagggca gcggcggctg cggcagggga
     6661 gggaagggca tggagccgtc tcatggtttc cagcgccagg gctgcgtttt cccgctgatg
     6721 ggctccgggc aggcatgggc ggggcgtgtt ccggtccgcc cgggcaatcg tgaggggaga
     6781 acggcggttc cgcgccgtcc gttccatgac ggccatgacg tccgggtgct gcacggcggt
     6841 tacggacggt ttgccggggg cgatgatggc cgctttttcc gcagcgatct cttccagggt
     6901 atctcccaga tactgctggt ggtccaggcc gatgggggcc agtacggcga tatccttggg
     6961 cacggcgtta gtggcgtcca gccttccgcc cagccctgtt tccagaatga tgaagttcac
     7021 gctgtttttc cggaagtggc gcagggcgac ggccagagcc agctcgaaaa aggtgggcgg
     7081 ctgttcccag ccttccgcca gctgtttcag gaaggaaatt tcttctgcca gggcatccgg
     7141 agtaatcatg ttcccattga cgcggatgcg ttcggaaaaa tctaccagat ggggggaggt
     7201 gaacaggccc gtgacataac cctgtgccct ggccagggcc tcaatcatgg cgcaggtgga
     7261 gcctttgcca ttggtgccgg ccacatggac cacggtggcg ttgatacggt ccgccccggc
     7321 tgccgccaac agtttttctg tgttgtcaag ccccagcttg atcccgaaaa attgggtgga
     7381 aaagagccag tcaagggcgg cggatacgtt catgggcggt attttaccac cgggaaaact
     7441 cccgttcaac tccggtatgg cgtggggact gtaaaatgcc gccgggaacg gacgcggcaa
     7501 acacgaacaa gctaccgttg ctacctctcg gtcctggcgg ggtttgccag tcgtgcctcc
     7561 acggggttcc cggcggccca cccagtaaac taggtttgca gtgcagtgtc aaccttcaaa
     7621 gacatcatct ccttctggcg aaagagagca gaatggagat cagcaatatt acgccgatga
     7681 cggttgccgc cgtgaaaaaa atctcttctt cctgtctgag ccggctgcgc tcttcttcga
     7741 ttttcgcgtc tctgacgcgt tgcagcccgt gcagggcttt ggcctgttcc tctacatccg
     7801 cctccgttcg catgacaatc gtttcctcat ggctgacggc ctggtcgtaa ggagcaatca
     7861 tcgtgcggaa ttcagggtct tccgaaagta tttccgtctc atcataatct tccgccagag
     7921 gggaattcat ggagtcccga aggctggcga tctgccggtc cacgttgttt tggaacaggc
     7981 ccagctccct tttggccttt tcgtaagctt ccgtctggtc gcgcagagcg gcttccccct
     8041 ggtcggtaat gcgctccagt tcctgcggaa gaccggcctg aagtttgagc agatccgtcc
     8101 tgttctccgt cactttaaaa ttcctgtttt tcgtgttgga cgcctccgcg agctggatgc
     8161 ggttgatgtc ctgacgggtt tccacctggc gtatttgttc ctgaagccgg tttcgggcgc
     8221 tgtccaattg ttgtctggtg gcgtccctcc tgcttttgag ttcttccact tccgcaacgc
     8281 gccggtcata ttgttccagc agatgggcta ttcgggcttc cgcgcttcgg gcattgggct
     8341 ttccgcttac gggagagggc gtctttttcc cgtctttttc ctggccggct tgctttattc
     8401 gggcatccca gtatttgcgg acggcttctt ccttccgttg aatagctttc tggagacgag
     8461 ccgcctgctg ttcgtgaagt tccgcggctt catcaaaacg ggcttgggcc gcatccagct
     8521 ccgggcaggg ttcctgctcc atggctttga gggcttcatg cctgggtgcg aggccgtttt
     8581 gataataatc ccagccgtag tatcccccgg cgagcaacag gatgagaagt aggtaacgca
     8641 tcaatgaaaa aaagggtaga gtcagaagca aatctcatag tgggagttcc gccggatttt
     8701 gcaaactcta aaatcagcca tccggagctt tttccctcag tagggagaat gatgataaat
     8761 tcactccgga gggggcgtag ttttccttga agccatactt tcaagccatg tctattttct
     8821 catcacgcag acaatttctc aaatctttgg ggcttgcggc cggagcggct gccgccggaa
     8881 atgccctccc cgggaaggct gtggaaatcc ctgccggaga ccatctctgg gaatctgcct
     8941 ccccggctgc tccgaggcct tccggttcca catacatggg agggttcaag gctccccggc
     9001 tgggtcgcat caggctggcc ttcatcggcg tgggagggcg cgggttctcc cacctggcgc
     9061 aaatgtgcgt gatggatgga gtggaaatcg tgggcatatg tgatttgaag gaagagttga
     9121 cgaaacgcgg cgtggatcgc gtgctctcca gaatggggaa aagccctctg ggctattccg
     9181 gcggcgatat ggaatacctg accatgctga aggagctgaa gccggatgcc gtcatcatca
     9241 gtacggattg gagttcgcat gccagaatag cctgcgacag catgaagcac ggcgctcacg
     9301 cctttgtgga agttcctctg gccgtctctc tggaggatct ctggagtctg gtggatacca
     9361 gcgaggccac caggaaacat tgcatgatga tggaaaacgt caactatggg cgggatgaac
     9421 tcatgttcct gaacatggtc cggcagggcg tcatcggcga cttgcttcac ggggaggctg
     9481 cgtatatcca ttgcctggtg acgcagctgg gggacacgcg tggggaaggg gcctggcggc
     9541 cggaatatca taccaggatc aatggcaacc tgtaccccac ccacgggttg gggccggtgg
     9601 ctcaatatat gagtttggag cgtggagagg accgtttctg tcgtgtggcg gcgttcgcct
     9661 ctcctgctct cgggcgcaat gcctacgcta aaaagcatct tcccgccgat caccgctgga
     9721 acaatactcc attcatctgc ggtgacatga atacggctgt tgtcaagacg cagctggggc
     9781 ggaccattct tgtccagctg gatgagacgt ccccccggcc ttactcccgc gccaacctga
     9841 tccagggaac ggagggcacg ctggccggtt tcccaacccg cgtggcgggt gaaaagctgg
     9901 gcaacggcaa ttatcatgaa tggattgaag gcagggaaaa actggccgct atttacgaaa
     9961 aatacgatca tcccctctgg aaacgcatcg gggagctggc cacgaaaatg ggcggtcacg
    10021 gcggtatgga ctttgtgatg ctttcccgca tcgtggaatg cctccggaac ggagaaccaa
    10081 tggatcagaa cgtttacgaa ggagcttcct ggtcttccct gctgccgttg acagcccgtt
    10141 ccatcgccca gggcgggatg cctgtggaat ttccggattt tacccgcgga gactggaaaa
    10201 ccaccatgcc gctggccgtg gtttcatgat gaaggggcgc tgccctcttc cggagagttc
    10261 aggaaggaat ccgttcttac gcagggtgct cagcagggac tgccggaaaa cagattgttt
    10321 aattcttccg cgctcatgtt catgagccat tgttcggggc ctccggagaa caggtcgtcc
    10381 gccatgcggc gcttgtcctt gagcatggcg tcaatgcggt cctcaatggt gccccggcag
    10441 atcaggcggt gcacgagtac gttccggtgc tgcccgatgc ggtaggcccg gtccgtggcc
    10501 tggttttcta cagcggggtt ccaccagcgg tccacatgga tgacgtggga tgcctgggtc
    10561 agcgtcaggc cggttccggc ggctttcagg gacaggatgc agaagggagg gccggattcc
    10621 ttctggaagg cggtgacgat gttctggcgt tccggaatag gcgttccccc gtgcagggta
    10681 agcccggaac ggccgaagac tccggacagg agatcgtgca gatggggaat gatggagcgg
    10741 aactgggtaa agagaatggt tttttcctgc ctggcggcaa tggaggcgca cagttcctga
    10801 agccgccgga actttccgga gcgttccggc gcataatcgt ccgtgccctg gaactgggcg
    10861 gggtggttgc agatctgctt gaggcgtgcc agaatgggta aaatcagcat caggcgggca
    10921 gctgggtccg gttcgtccag tacggcatgc agcatgtcta tctgtgcttg gtacaaggcg
    10981 gcctgttccg gcgtgaggga gcaataggcc ggtatttcca ttttgtccgg caggtcggga
    11041 accagcgccg gatccgtttt cattctgcgc aggatgaagg ggcgcaccag gcgtcgcagc
    11101 ggggcataac cgcgttccag ggaacgggtg aaggattcga agctttttct gctgcccagc
    11161 agccccggat tcagaaattc catcaggctc cagagttcat tcaggttatt ttccaccggc
    11221 gtgccggaca gggctatgcg tcgttcccca tgcagggaac ggatggcgcg ggaacgggcg
    11281 gagcctgcgt ttttaatagc ctgggcttca tccagaataa tggccgggaa atgcaggtcg
    11341 gccagggcgg acgtgcgtgc agccatgcca tacgtagtga tgacggcgtg gcagtctttc
    11401 cggcgcaggg tattttccgg ctgccacggg tccggggagg aagggtgcat gatgcgcagg
    11461 ataagctccg gagcgaagcg ttgcgcttcc tctttccagt tggacaggag ggaggccgga
    11521 gccacaatca gagccggcag accctccagc cggccttttc tccgcaatgc gtccagccac
    11581 gcaatagcct gaagggtttt tcccagcccc atatcatcag ccaggcaggc gccgaaaccc
    11641 cgttccgtaa tccggtaaag gaaagagaac ccttctttct ggtaagggcg catcagggcg
    11701 tccagccggg aaggaagctc cggtatggag actgggactt tccccgccag gatgtccagg
    11761 gtctgccgga ggttttgtcc cggttccatg acacagtctt cgtccggttc cggaatctgt
    11821 acaagctgga cggaagggcc attaaccagc attctcagac cctggaccag aggaatgccg
    11881 agggagctca tcatacgtgc cgcttgagtc caccggtcca ttaagtcccg gaccttccgg
    11941 gcgtccacgc gcacccattc ccccttgaag cggatcaggc cgtctccgtt gctgagcagt
    12001 tcccccagct cctccggggt caggtctttg cctcccagag tggcggatac ggaaaatttc
    12061 agcagactgc ggacggaaag cccggaagaa tggaccgttt tttcacttcc ggatgcgtct
    12121 gccgtaactt ttacctgtag tttgggaggg gggtttttcc atagattcac catgcgcaca
    12181 atgatgcctg cgcgttcgta gagcggcgtg tcctgcagaa aatcccgggc ctccggagcg
    12241 ctccaggcca tcggcgtata aatttttccg ttatccagca ggcgcttcag cagggggctt
    12301 tcccgtgccg cttctttcaa gggctggagt acggccagca aggcggtgta attcccttca
    12361 tgaagtttca gggcggtggc aagggggagg tggcgcacct ggttgtccgc agcgctttga
    12421 tgcgcaaagg tagccatgaa agcaaagggg cgagagcctg tggcgtcatc ccggttttcc
    12481 gcaaggtgga aaaatacctt gcctatctgg ttccagggcg cgcccaggct gttcaaccat
    12541 tcggcggggg taatgcctcg ggcgcggatt tcctttgcta ttgccttccg caaatcttca
    12601 aaccatccgg caatgatgtc cggagtggca tattccgcgc ccggcacggg ggggaattgc
    12661 tccgccagaa aggctttttc cgtagcggag gccagcaggt ggtccagaac ggcatccacc
    12721 tcgtccggcc tttctgcggc agcgtgcgcc atgcgggtaa gcgcctggcg cgccatgttc
    12781 ttgataaaga aggcgcccgg atccggaacg gtttcccagt tggataccgc cagggcatac
    12841 agcccgtttc cggttccttc ttcaaacaga cggtcgaaag gcggcgtgtc cgtcaccaaa
    12901 gcaccggagg ggaggatgcg gaaaccattg atattccgtt tctcttccgg tactgcatga
    12961 tagggggccg gctgcatggc tggcgggaat gtagcgtgtg cttgcggccg tagttcacag
    13021 ggcgaacgtc gtatctctga tgatgggcag cttggacggc ggaggattga gcagcttctt
    13081 gatttgtttc aggtaatcaa tcagggcttt gcggacatct attcccgtcg gtttgccgtt
    13141 tccccgttgg ggaaatatga aattgctgca caggtagtct cccatcgcga cagtataaat
    13201 gaccccgttt ttcaaagtgc tggtaatgct gggcgtaaca ccgtccatga tctggtagga
    13261 gaatccggag cagtacactt ccagaatacc tccttcgtca tcggtcggag acatgaattt
    13321 ggacaggatc agttgctcaa tgtccgcttt actcatggag caggtgacga tcttttcccg
    13381 gaagggttcc agctcgtaaa tgtccctgat ggtaacgggt cctttgttaa gatggttttt
    13441 ggaccgtact ccgccgcggt tgtaaatggc aatgtccgaa tgggaggcct gctgaatggc
    13501 cttgcagaaa agggtgccta tggtgacgtg cgtgatttct tctccggcga cgcccacctg
    13561 ctggttgaaa agcggatttc cggaaaaccg gcggacaatt tcctttactt ccgggtcatt
    13621 gggaatttcc tcattcaaag aaacggccct ggtggatttg gaaaggatgg agacgggatg
    13681 ttccgtggag aagatgattt ccgtaactcc tacgtggctc aaactatgtc ccgtgtggct
    13741 gagcagcgtg cctgttctga gatgaccgct gggaagcatg acatgggtat ggcctccgag
    13801 aatgacgtcg atattcggcg aatacttcat cattttcaga tcattgccgt atcccagatg
    13861 gctgaggatg acgttaatgg tgttgtggtg aagccggaac ctgtccgtga tgcccttgta
    13921 atcttcttca tccggcagcg tccatgagat gccggccatc ttcctcacat ccgtggtttg
    13981 aaggtccgcc agacctatga accctacgga aacggacgtg ccttcaatcg ggatgctgac
    14041 atacggagag aaacagtttt ccagagtagg ggaaagattg gcgttggtaa tgacaaactt
    14101 ggtggaaggc attcccttga tatggtcgcg cagggcctcc tgcccgtagt ccaggtcatg
    14161 gtttccgatg gtcactacgt cataccccag tttattcatc aggatggtaa ggggttcgcc
    14221 ccgtttttca cagtcgtcca catagggatt acctatgaaa taatcgcctg aatccaccag
    14281 cagcacgtgc ggatatttag cgcggtattg ttttaccagt gtggccagtc ttgggaaatt
    14341 gtccagattg ccgtggatat cgctggtaga aatgataatc agcgttttat cgggatccga
    14401 tttaggctcc tgagccagag agaacgggca caggcatgcc agggaggcaa gaacaatgcg
    14461 tagcggaatc atgcagggta aaatgataaa tatacggaaa aggatgaaat gggattaacg
    14521 ggcggaaacc tcatgcgggc cgtcacccac ggagatgact tccagacgga ccctggatac
    14581 gccgcgtcta tgcaggccga tgcggcttgc ggcggcggtg gtgaggtcta tcaggcggtt
    14641 gctgtgaaaa ggcccgcggt tgttgattct gacgacggtt gatttgccgg tttccaggca
    14701 ggtgactttc accttgcacg gcatcggcag ggttttgtgg gccgccgtca tggaacgctg
    14761 gggattgatg tactctccgg aagatgtctt caggcggcgc gctttgccgc cataccagga
    14821 ggcatagccg gtctcatggt attgcagggc atccgccacg ctcatgggag tatacctttt
    14881 tccacgcacc cggtagggcg catttttgac atgcgcctct gcctcctgaa cggacggctc
    14941 cgttttctga gcgcacttct ccgtagtgga gcagcccggc atcagaaaaa acagcaggac
    15001 ggggaagaac catttcacgc tttttacgtt taacacatat tgatgatttt gtataacaaa
    15061 aaaggaacaa gagttgaaat ttaaggaata gagcagggcg gaacaatctt ttcccggttg
    15121 cggtttacca gttgagcggc tcggaaaaat gcagtatgat ctcccgcatg aattcttcca
    15181 aggtaatcgg cctggtcgcg ctggcggaaa aaccgggact gggggaagcg ttgcgcatca
    15241 tgcgccggga actttcccgc cacgggctgg gctcctgcgt cattgaaacg ccggatgccc
    15301 tggaacgggc tatcgggcgg tgcggcatgc tggtgacctt cggaggggac ggcaccatgc
    15361 tgaccgtctc ttccctggcg gccatgcacc gcgttcccct ggcgggagtg aacctgggcc
    15421 ggctgggatt catgaccacc tgttccgtgc aggaactgcc gcttctggcg tatgcccttc
    15481 aggaaggttc ctacctgacg gatgaacgtt ccatgcttga agtagtacgg gcaggggaag
    15541 acggcgttgc ggctcctccc cgcaagctgg ccttgaatga agtttccctg atccgcgccc
    15601 agtccggcaa gatggtggac ctggatgcgg agatagacgg ggaattgctg aaccgctacc
    15661 atgcggacgg cgttctggtt tccactccca ccggctccac ggcatactcc ctttccgccg
    15721 gggggccttt ggtctggccc atgtcccgtg tcgtatgcgt cacccccatt tgcccgcaca
    15781 gcctgaccaa tcgttccgtg gtgcttcctg acaacatgac catacgcctg cggccccgcg
    15841 agcgccgcgg ccgctttgac tccatggttt attccctgga cggccgttcc gcctatccca
    15901 ttgaagtggg agaaagcctg gtaatccgga aggccccgga aacgctttcc ctggttcatc
    15961 tccggaagca gaatttcggc gccctgctga gggccaagct gcgctggcag ggggcggaaa
    16021 tcccctcgga cgatgaatag gccgttgcgg aagcccggcc tccggcgggc taccccctca
    16081 ctacggaatt cccgcatccg ggaacagtcc ttcgttcatc gttccgcatt ggcgcagggg
    16141 cggtaaaacg atttttttag gatgcgggct tgacgaaaag gcctatccta tcatactctt
    16201 cctgccctgc caccaagggg gtggcagatc ttaatcgcat aaacgcgtaa gaaatagtca
    16261 tatatgaaga cccacgtgaa aaaaggtgat gaagtggaaa tcatcgccgg acgtaaccac
    16321 aagggcaagc gcggcaccgt tctcgccgtg aatgcttcca aaggtactgt catcgtagaa
    16381 ggcgcccgca ccctgaaaaa ggctgtgcgc ccgaccgaac agaacccgga aggcggcatt
    16441 caggaaatca acggcccgat tcacatctcc aacgtgaaaa aagtaggcta ggccatccgg
    16501 tctgaccctg cgcttaagcc atatcaatca ttaataaaga gctgacccgc taaaacaatg
    16561 agtacaccaa cactgcaaac atactaccgt gaaaaggtcg ttcccggcct tcaagcgaag
    16621 catgcataca agaacgtgca tcagattccc cgcctggaga agattgttgt cacctcctgc
    16681 atgggcaagc acccggaccg caagcaggcc gtggaagatg ccgttaatga aatcgccaaa
    16741 atcaccggac aaaaaccctc cgtcacctat gcccgcaaga gcgtggccaa cttcaaggtg
    16801 cgtgaaggcg aacctctggg agcccgcgta acgctgcgcg gcgcccgcat gtgggaattc
    16861 ctggaccgct tcatcaatat tacggctccg aacattcgcg acttccgcgg tcttccctac
    16921 aagtcctttg acggcaacgg aaactatgcc gtcggcatct ccgaccagtc catcttcccc
    16981 gaaatcgaac ttgaccagat caagcgtcag atcggcttcg atatcatttt cgtgacttcc
    17041 gctcctacgg acgatgaagg ccgcgacctg cttcgcgccc tgggcgtccc catgcgcgaa
    17101 ccgaagaagg ccgaagaaac ccaaactgcc aacgcctaac tatttcttac catggccgta
    17161 ttaagtgatc ccatttccga cttcctcacc cgcctgaaaa acgccagcct ggccggcaac
    17221 gaggaattta ccgctccctg ctccaacatc aaggtggaaa tcgcccggat tctgcaggaa
    17281 gaaggctaca tctggaacta tgaagtgacc ggagaaggct ccaagaagca aatcaaggtg
    17341 aagatcaagt ttaccccgca gggcaagccg gtcgttaccg atgtgaagcg cagctccaag
    17401 ccgggccgcc gccagtacgt ttcttccgag gacattcccc gcgttctcag cgggcttggc
    17461 attgccatcg tctccacctc ccgcggtgtg atgaccggtg cgcaggcgcg caagcagagc
    17521 attggcggcg aacttctcgc ccttgtatgg taaccattaa catttcctaa acatatgtcc
    17581 cgagttggta aaaaatccat cacattgccg gacaaggtaa cggtaaaggt taacggctct
    17641 tccgttcagg tggaaggccc caaaggaaag ctttcctgga cgcttccgga aggcatcacc
    17701 gctaccgttg aaggcaatca gctctccgtt gaccgcgtcg gcgaaagccg tcagcttcgc
    17761 gccctgcacg gtacgaaccg ttccctgttg aacaacatgg tcatcggcgt gtccgaaggc
    17821 ttcgtcaaga accttgaaat cgtcggcgtc ggtttccgcg ccgccgtcaa gggcaacatc
    17881 ctggatctga acctgggcaa atcccatccg atcaaccagg taatcccgga aggcctcaag
    17941 gtaaccgtga ctgaaaacac gaaagtgacg gtcgaaggca tcgacaagca ggtcgtgggc
    18001 cagtttgccg ctgaagtccg tgcgtactat cccccggaac cctacaaggg caagggtgtg
    18061 cgtttcgtcg gtgaagttat ccgccgcaag gaaggcaaga gcgtcggtaa gtaattcagg
    18121 taataactat tacaacatat ttacacacga tgagtactat caatcgcaaa gccatccgcg
    18181 ccaaggttcg ccgccgcatc cgcagaaagg tttccggaac ggcttcccgc ccccgcctgg
    18241 ctgtctattt ctccaaccgc cacgtctatg cccagatcat cgacgatacg gtgggcaaga
    18301 ctatctgctc cgcttccacg gccgatgctt ccatcgccga tccttccaaa ctcgccaact
    18361 gcgctaccgc taccaaggtg ggcaatctga tcggcgaacg cgcgaaggct gccaatgtca
    18421 ctgaagtcgt tttcgaccgc ggtggattca agttctgcgg taaggtgaag gccctggccg
    18481 atgccgcccg cgaaaccggt ttgaaattct aattgaaaga ttccctaaat gaataacgaa
    18541 acggaaaaca ctgctcctgc tacggaagcc gccgctcccg ctcaagcgca gcaacccgca
    18601 acgggcggcc gcggaccccg cggaccccgc cgtgacggcc agaatggccg cggaccccgc
    18661 ggaccccgtc gtgacggacg ccgtggccct caggctgaag ctcccgtgga agaaggcccc
    18721 cagctcgctg aaaaggttgt gttcatcaac cgttgcgcca aggtcgtcaa gggcggccgc
    18781 cgcttctcct tctccgccct cgtcgtgagc ggagacatgg aaggccgcgt aggcttcggc
    18841 ttcggcaagg ccaatgaagt ggcagacgcc atccgcaagg ctacggaagc cgccaagcgc
    18901 cagcttcgcc cggtctccat cgccaacggc acgattccgc atgaagtcta ctccgaattc
    18961 ggcggcggca aggtgcttct gaagcccgcc tcccccggta ccggcctgat tgccggcaac
    19021 accatccgtg ccgtgctgga agcagcgggc gtgcgtgaca ttgttgccaa atccctcggt
    19081 tcctccaacc acgccaacgt ggtgaaggca accctgcagg ctctcgcttc cctgcgcacg
    19141 agcgaccagg ttcttgccga ccgcggcaag aagaaggaaa aggccatcta atctgatcag
    19201 gaggcggcgg cccggcgccg ccgcctccac gaaatccaac cattgtaaat aacatgttac
    19261 agcttcatac gattaagccc aatcccggag ccaagcaccg caagaagcgc ctcggcaacg
    19321 gggaaagctc cggtctcggc aagacctgcg gcaaaggcaa caaaggccag aaggcccgct
    19381 ccggcggcac gattcggccg ggctttgaag gcggccagat gccccttcac cgccgtctgc
    19441 ccaagaaggg gttcaacaat acccgcttcc aggataaaat cctcattgtc aacctctccc
    19501 agcttgaacg cgtgtttgaa gccggcgcta cggttgatga aaacaccctg cgtgcagcca
    19561 agctcgtcca aggtccctgc gacgccgtga agcttctcgg caacggcacc ctgaccaaga
    19621 acctgaacgt agtcgtggac ttcgtgagcg ccagcgcccg cgagaaggta acccaggccg
    19681 gtggcaccgt caccgtgctt agcgaagcct aatcacattt ggcgaaccgg gtcatgcagc
    19741 ttgacccggt tcgcatcatt gtgtttactg ttgcggttcc catggatgcc ccgggtgcaa
    19801 accgccgcga catgcaagcc aagctgcgcg ccaactt
//
LOCUS       DAOMSU010000008        19533 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_73_length_19533_cov_3.227744, whole genome shotgun sequence.
ACCESSION   DAOMSU010000008 DAOMSU010000000
VERSION     DAOMSU010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 19533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19533
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_73_length_19533_cov_3.227744"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..946)
                     /locus_tag="PK077_00820"
     CDS             complement(56..946)
                     /locus_tag="PK077_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22874.1"
                     /translation="MFKEPSFLKKANLFQPILVVVISIIGGTPMEGANTGKIVLLIIL
                     GAAFIVWHVECQRLLNRNRETREHACALKNRSAAPYKKTYAALSGEYENFANKLNGES
                     CREAKTDGSRGTTESFNGACLFLCSTIAAALAEYKNSLVFEVLYIQAERRKDQTLLHV
                     TGYAQGEHNNDTPSLLAQPPRPVTKTPAMLDEQLFAERHLSPLVLSSPEEMQRSFFRK
                     RNQPPEEKYMQYLAIPVLSKRNEIIGLIEIAVKKNPFISPVTMLEAHELADVKSWLYH
                     LKDHFLLFHEIERAVRDGFP"
     gene            complement(1564..3156)
                     /locus_tag="PK077_00825"
     CDS             complement(1564..3156)
                     /locus_tag="PK077_00825"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRN22875.1"
                     /translation="MIFHIPFMCSTLRSSIMSSIAFWLLTLVPSLADRPNIVLILADD
                     MGWSDPGCYGSEIPTPALDTLARQGMLATRLYTASRCSPSRASIMTGGEPHKVDVGLL
                     DDDSGRPGYRGRLNPGIPTLPELLKKAGYRTYLSGKWHLGKVRGSYPWDRGFDRSRGL
                     LGGAADYYRPMPDRPFGENGKLLRPEDLPEDFYMTDDITKTALAYIDDAVKSRQPFFL
                     YVAYTAPHTPLQAPRREIEKMLPFYNGKSPHAIASKRLEKQKLLGIVPPAAKLGMAGK
                     FNPEGYEKTSAKRKDYIAECMATYAAQIVIMDRGIGRILASLERHRLSDNTIVMFLSD
                     NGATAEMPQNNKNKKTTLPTGPLGEVGCKDGYGPMWAAVSNTPYRQYKIETFDGGLSA
                     PFIIRYPSKIRPESRYHSPFLLQDIAPTCLAWAALPIPAHMDSKPLNTYWNNPPKLPP
                     SKVWDFIPNTCPPRTIFWEHQRNRAALTSQFKLVAPNRGPWQVYDIRDRTEQKNLASR
                     HQTLVEQLSAQYRKWAAENHAE"
     gene            complement(3316..3888)
                     /locus_tag="PK077_00830"
     CDS             complement(3316..3888)
                     /locus_tag="PK077_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22876.1"
                     /translation="MIPLSVAGYILGTVLLLVHLYALVKRQQVQAFLLASPRNHLLAQ
                     ILLAGGLFWFFLLVAPEGLGVLSRFRVGLAEFEGIRWLLQLACPVFLVLMVTQVKNLL
                     FPRALGIFGLMVAAPLLSAAFLQEPVTRLLIPIWCYIVIFLSILWIGKPYLYRDMVNK
                     ICSKPAWWTPLCLGGMAYGAAILLCAILWW"
     gene            complement(4058..4741)
                     /locus_tag="PK077_00835"
     CDS             complement(4058..4741)
                     /locus_tag="PK077_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRN22877.1"
                     /translation="MKTRLLTPRSKGFTLIEVLVVIAIIALLAGVSYSIYSHATETAA
                     RTRCTDNLRRISDWGKEFAGQNGGKLPSSGMKDSLLSARECRNWWDALAPIVNAEQPD
                     LIARNAKEPNMLPDTFRCKNDKRPEILGAEDANLPAGPDTISYTSWLDNRKGRPMNVA
                     RGQALRGKPWISDGIPIDGRSVITEQDFEEIVVPALERHQGGIMVLYADGKISPVEDP
                     TLNKVTQGS"
     gene            complement(4889..5653)
                     /gene="tpiA"
                     /locus_tag="PK077_00840"
     CDS             complement(4889..5653)
                     /gene="tpiA"
                     /locus_tag="PK077_00840"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRN22878.1"
                     /translation="MSRKPIIAANWKMNIGPAEGAQFIESFKNLIKGKDVACDVVIIP
                     PFTTIPSVQNALGGCSCITAGAQNVSQYDNGAYTGEISTSMLNELNLKYVVLGHSERR
                     QYFGETDAIINSKIKKAIAAGITPIFCIGETKDERLGGILEPVLEIQLKGGLKDLTPE
                     EVSNLVIAYEPVWAIGTGLTATSKEAQEAHSFIRKVISDVFGADAAAKVRIQYGGSVK
                     PENVEELMAQPDIDGALVGGASLKPESFAALVTSAK"
     gene            complement(5706..6944)
                     /locus_tag="PK077_00845"
     CDS             complement(5706..6944)
                     /locus_tag="PK077_00845"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRN22879.1"
                     /translation="MKNNPPSPRQTALNCLRSWHAGRSFAETLVDRECSRAALPPADR
                     HLVQALVFSVLRNQTWLDHVIGTLRKGKLDVEARLILQLGLSQLFLLGMADHAAVYET
                     VNLASVRLRGLVNAILRNALRREKTILEDREKLPLSIHYSTPAWLVQRWTEQMGPQMT
                     RDLLRWNNTTPRLYVRANPLIPMKNIPASLAPLDRAPGWFSVEGLLPLEEIKTGSLYV
                     ADPSTRYSIDLLAPQPGEEILDACAAPGGKSAAIIAATGGKARLTATDLHKHRLPILK
                     ENLDRQGSPFVRTAQADWSLPCRTEWKGRFDAVLLDVPCSNTGVIQRRVDVRWRLTPE
                     EIRRLAALQKTILENASRAVKPGGRLVYSTCSIDAEEDGLLVRDFLQNHPEWTLKEEK
                     LILPHEEKSDGAYAALLICA"
     gene            complement(7031..8536)
                     /locus_tag="PK077_00850"
     CDS             complement(7031..8536)
                     /locus_tag="PK077_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRN22880.1"
                     /translation="MASAAAFKPNIIFIYADDLGMGMLGCYGQEIVKTPNIDRLASQG
                     IMFTRCYSSQYCCPARASLLMGVHDSHSHSYTQTAGALVITAEREGWSNEQLEEKAAR
                     AVRIKAAEGEVFLPELLKKAGYVTGQFGKLEWGFTTWHGELKRHGWDRYVGYMDHQRA
                     HGFYPSFLWKDGERLPLKGNTHADGGKTPEDYSPGATEKRRGNREGKVTYAPDVMLAE
                     TLRFMEENRNRPMFIFFSTNLPHGPVDIPPEENTYAGSPAIRQAYGNASGTNRECSGA
                     AEEYASMVDKLDRQVGAIVDQVRKLGLDKRTIIIFSSDNGHELYYRTDKGRGRGPDCH
                     GGVLDGTGELLDVFRGSRGRVGPNNAMANLAGPKWTSHEGGIRVPLIISWPGTLPHGK
                     VCHSLVANYDHMASFADLAGIRMPEGKDAVSYMDILSGNSSRQRDYVVVDHTVITGDG
                     WKLTQKQNKPFLFHIGRDPEERNNLAGTRKDQLEKLRAIYRKEVGSPRKDR"
     gene            complement(8692..9984)
                     /locus_tag="PK077_00855"
     CDS             complement(8692..9984)
                     /locus_tag="PK077_00855"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HRN22881.1"
                     /translation="MKIVVAYSGGLDTSVLLKWLKEKYNAKIIAYCADVGQAEELDGL
                     EAKALATGASKCFIGDLKEDFATNYIFPMMQANALYEGRYLLGTSIARPCISKDMVDL
                     AIREGADAIAHGATGKGNDQVRFELAVNALAPNIKVIAPWRDPEFRQQFPGRTEMIAY
                     AESHGIPIRQSLKKPYSMDRNLLHISFEAGMLEDTWYDGTTPADREMYKLSVSPEDAP
                     DQAEYIQLLYEKGNVIGIQYDGLDVLLKELGVTPKGERDGYTLLSPLGVMYVLNALGG
                     KHGIGRVDIVENRFVGMKSRGIYETPGGTILLAAHRDIETITIDREVQKVRDSLIPEY
                     ATLVYNGFWFAPEREAIQALVTKSQETVNGEVRLKLYKGNVMYAGRRSPQSLYSEEIA
                     TMEGGHEELYNQNDAEGFIHLNGLRLKQFSRVNKPYGN"
     gene            complement(10263..>10547)
                     /locus_tag="PK077_00860"
     CDS             complement(10263..>10547)
                     /locus_tag="PK077_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22882.1"
                     /translation="LISVPAVNIEEKRHGEFCLHAPPVSDPLRNQIHHQGGQDRELHH
                     LRRLAAEPFTGQASAGNFRESMQKERKIWLSLARYGYLYIGKRLFHSCSG"
     assembly_gap    10548..10581
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            10931..11200
                     /gene="rpsN"
                     /locus_tag="PK077_00865"
     CDS             10931..11200
                     /gene="rpsN"
                     /locus_tag="PK077_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HRN22883.1"
                     /translation="MAKKSWIARDKKKAETVKRYAELRAQLKAEKDYIGLTMLPRNAS
                     PTRTVNRCLVSGRRRAFIRRFKLSRISFRELANAGMIPGVTKSSW"
     gene            11273..11533
                     /locus_tag="PK077_00870"
     CDS             11273..11533
                     /locus_tag="PK077_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRN22884.1"
                     /translation="MPIYEYISENPDDPGQSCPVCRRGFELRRPVDRAPLEKCLVCKH
                     PVRKVISRINVPEVTKPLSVSDAKAAGFTVLERRDKGVYEKL"
     gene            11592..13004
                     /locus_tag="PK077_00875"
     CDS             11592..13004
                     /locus_tag="PK077_00875"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRN22885.1"
                     /translation="MNDPDPLSILAAFGAAEAPGAGPALGGMMLAVAGFILFLLLNAF
                     FAAGEFALMKVRESQLHAGEGVPARTRKKLARARKAAKHPDLYLAACQAGITLSSLAL
                     GFLGTFFVSELTAPFLVSLGLGGMVSVYGIALAVTFIFFACCQVVFGEFIPKAMAMRQ
                     PDKAALATVPLLYFFYTVFRYTGILGLTGGMARFVLKYLLGIDPRSTACTVHSTDELM
                     YLVEESERSRELTKQEAEISKNALELNDMCVKDVMTPRSEVDVMDLTAPFEENWELAR
                     KSRHTRFPLVEGDHLDEVKGWVHVKDLLKLVGRENPDLRSVRRELRVVPDTMPLDSLL
                     TFFLKEHAHFALVVDEFGDSIGLVFLDDVLEQIVGDDIQDEFDQEEMREFVKTGKDTY
                     AVNGAITLFDLADYLPEMDLDCPGVTTLGGYVISRMGYIPEEGEELRIGRYRAVVTGS
                     DGRRITQILLARLPEEQEEE"
     gene            13033..13653
                     /locus_tag="PK077_00880"
     CDS             13033..13653
                     /locus_tag="PK077_00880"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRN22886.1"
                     /translation="MSFEMEARASGFLCVAGVDEAGRGPLAGPVVAGAVILPVLAEEL
                     TGLNDSKQLTAAKRERLFLALLECEQAVCSVGVASVEEIDRFNILRATHLAMARAVEG
                     LALRADFCLVDGLPVKGLPVPHRAIVKGDGRSLSIAAASVLAKVTRDRMMMEADASYP
                     QYGFAKHKGYGTKAHMEALRRHGPCPLHRRSFAPVSQMELSIPEQG"
     gene            13676..14032
                     /locus_tag="PK077_00885"
     CDS             13676..14032
                     /locus_tag="PK077_00885"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRN22887.1"
                     /translation="MYGELAAASFLRAEGCVILRRNWRPVRGGELDIVCREGECLVFV
                     EVKTRTGNGYGGARRAVNARKRALIRRGAAEWLRLLPEPVNSRYDVVEVLYREGMPPE
                     FRHIRGAFGAKDLPAS"
     gene            complement(14185..14970)
                     /locus_tag="PK077_00890"
     CDS             complement(14185..14970)
                     /locus_tag="PK077_00890"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22888.1"
                     /translation="MDYLNSILERKRADLKKLLPLEGKLRASAIQRNDYAGFRTAADL
                     GETRLSVIPEISRVHPALRLREAAQDIRHMYGMFLQGGAQGISIAVEPEVYGGSWDMV
                     STVSRISSIPVMARDVFIHPAMICQAIVSGADAVNLVTAALPAKDLSELYRMASGLGL
                     DVMMEVHTLKELETVMDLEAEFICINNADPHTLQANPAVTEKLIEEIPASVTVLSAGG
                     ICTVEDARRMLDAGANGVLLQDELARMDIPQDFMEAILALRAD"
     gene            complement(14974..15948)
                     /locus_tag="PK077_00895"
     CDS             complement(14974..15948)
                     /locus_tag="PK077_00895"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRN22889.1"
                     /translation="MSAGLPQHLTLDYLRALLSRGVEKTTVTEEARMVLRKWVMDARR
                     ISGSPLPASVSAKPAANETRPKQPTPEQTPPDSVDEASFGNELRDIFNGVQPHRTEED
                     APVPRHISFDLEGETEEEKLSSLRELVVNWPPLRNMDSLRETPVFSSGNPKADIMMVT
                     DAPGLYEEKQGVPLAGPSGQKLDAMLKAMGLSRSDIYLTHLVKYRPALPRQLTNNRPP
                     TDREIEISLPILREEIMLVRPKVVVALGAISARGILQSGETPLSALRGTFHTAFNTPV
                     RVTYNPSYLLRTEDISEKRKVWEDMLCVMEQAGLPISEKQRSYFLPKK"
     assembly_gap    16424..16460
                     /estimated_length=37
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            16875..17546
                     /locus_tag="PK077_00900"
     CDS             16875..17546
                     /locus_tag="PK077_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009962941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HRN22890.1"
                     /translation="MNLRIIATLSCAVAAMFVSSCSTNGAVTGMGKSSSDALVLARVG
                     ETAQGFVQPLAPSRSAAPSSKLPKLGRDKHKMPFYHPAQRTRVVRTTAYTHSERDHLA
                     YGPRNAVGTALKYTSSVRSAAADWSVYPLGTTFRIKGQPYLYVVDDYGSALVGTGTID
                     IYQPNKKLMKEWGRRYVELTIVRWGDPANSLEVLGSRRGYRHCRAMYAALQHRVSKGF
                     YAKAD"
     gene            17634..18272
                     /gene="nth"
                     /locus_tag="PK077_00905"
     CDS             17634..18272
                     /gene="nth"
                     /locus_tag="PK077_00905"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRN22891.1"
                     /translation="MNTEKRASIVQEELMSLYGAPPIPLVHRDAYTLLVAVLLSAQCT
                     DKRVNLVTPALFALASTPEEMARQDVEAVREIVRPCGLSERKASAIVNLSRILVEKYE
                     GKVPCDFAALESLPGVGHKTASVVMVQAFGVPAFPVDTHIFRLSRLWGLSTGKTVETV
                     ERDLKSLFPEKSWGDLHLRIVLYGREYCPARGCGGRCPICSRLARESGCEGV"
     gene            18365..19156
                     /locus_tag="PK077_00910"
     CDS             18365..19156
                     /locus_tag="PK077_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRN22892.1"
                     /translation="MKNLFRLGFRFVAFASVLMAAFSTSAFAQEAAEAVAPQEQTMLD
                     KWIIAGGWTMIPIMLVEAFIIFLVIYNMVALKKEKFCPEDLKVTLLQLMAECRIRSAI
                     EVAAGSPTYLGRLVAYALPNVDATRPEDLGKDAIEDAVADFTANESRSVFKWINMLAL
                     CAQISPMLGLFGTVQGMVGAFGTLATAGQADPTQLAGDISVALLTTFWGLINAIIATP
                     FFFFQKGIANAQIAECVGTVQEMVNTSINVVNAEAQLARIPEGLA"
     gene            19289..>19533
                     /locus_tag="PK077_00915"
     CDS             19289..>19533
                     /locus_tag="PK077_00915"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRN22893.1"
                     /translation="MSPMIDMVFLLLIFFVVNATAITVKKDKNIQMPTASSSGEVKSA
                     NGCIVVNVYGEGAGKRPPGVDPSVLWSSDVGTPLNSS"
BASE COUNT         4029 a         4951 c         5793 g         4689 t
ORIGIN      
        1 aaatttttaa gggatatggc gttgggcgta gcaggctatt ctgtccgtac tgccgtcatg
       61 ggaatccatc ccggacggcg cgttctattt catgaaacaa caggaaatgg tcctttaaat
      121 ggtacaacca tgatttaacg tcggccaatt catgggcttc cagcatggtg acgggcgaaa
      181 tgaacggatt ttttttaacg gcgatttcga taagcccgat gatttcattt ctcttgctca
      241 ggacgggaat ggccagatac tgcatgtatt tttcctccgg aggctggttg cgcttccgga
      301 aaaagctgcg ctgcatttct tccgggctgg aaagaaccag ggggctgagg tggcgttccg
      361 cgaataattg ttcatccagc atggcgggtg ttttcgttac gggccgcgga ggctgtgcca
      421 gcagggaagg ggtgtcgtta ttgtgttctc cctgtgcgta gcctgtaacg tgcagcaagg
      481 tttggtcttt cctccgctct gcctgaatat aaagcacttc aaagacaagg gagtttttat
      541 attcagccag cgcagcggca atggtggagc acaggaacag gcaggctccg ttgaagcttt
      601 ccgtcgtccc ccttgaaccg tccgtttttg cctcccggca actttctcca ttcagtttat
      661 tggcgaagtt ttcgtattct ccgctcaggg cggcgtaagt ttttttgtaa ggagccgcac
      721 tgcggttttt cagggcgcag gcatgttccc tggtttcccg gtttctgttg agcagtctct
      781 ggcattccac atgccataca atgaaagcgg cgcccagaat gatcaaaagg actattttcc
      841 ccgtattggc tccttccatg ggagttcccc cgatgatgga gatcacgaca accagaatag
      901 gctggaacag atttgctttt ttgaggaaag agggctcttt gaacataaaa agacgctgtg
      961 caagttttgt taaaggtgtg ttatgaattg attatcaaaa atattccata tctggtgtga
     1021 cggcagtaca gaacggaggt aaggtgcaga aaggtttttt cttatggcat tttaaattaa
     1081 gtttatttac aatcaatgag ttttttgcta atttagaagt attctaatac ggtatttctt
     1141 tgaatgataa ggattctttt attccaataa ttgttaggaa aaaagaagaa ttttcctttt
     1201 gggaaatgat ttggtaaaca gcggtgtttt gtctagtttt aaccttataa aggaattatt
     1261 tttgattaca gggcaaaaaa tagggcatag gaaaaatccg aatgaagaag cggattttta
     1321 tgcaattccc cttcatggga aaggatggca aggagtgtga aaataatgaa tggttcccgg
     1381 aatgcccggg aggcaggaat gttgccgcgg acgggaataa ctgttattcc ctgctttttc
     1441 tttcccggtg aatcacgtgc cgttttccgt tacttgagga aacggaaatc agaattttct
     1501 tccggaaggg gattcccata gaaacgttta gggcgcgtat gcccattttc ctgccgtgcc
     1561 gtgtcattcg gcatggtttt ccgccgccca tttcctgtac tgtgcggaca attgttctac
     1621 aagcgtctgg tgcctggatg ccagattttt ctgttccgtc ctgtccctga tgtcgtacac
     1681 ctgccagggg cctcggttgg gggccaccag cttgaattga cttgtcaggg ccgcccggtt
     1741 tctctgatgt tcccagaaga tagtgcgggg agggcaggta ttgggaatga agtcccacac
     1801 cttggacgga ggaagtttcg gaggattatt ccagtaggtg ttgagcggct tgctgtccat
     1861 atgggccgga attgggagag cggcccacgc aaggcaggtt ggggcgatgt cctggagcag
     1921 gaaaggcgag tggtagcgcg attccggacg tattttggaa gggtagcgaa tgatgaaggg
     1981 ggcggacagc cctccgtcaa aggtttctat tttatactgg cggtaagggg tattggacac
     2041 agccgcccac atggggccgt atccgtcctt gcatccgact tctcccagcg ggcctgtggg
     2101 gagggtagtc ttcttgtttt tattgttctg gggcatttcc gctgttgcgc cattgtctga
     2161 taaaaacatg acgatggtat tgtcactgag gcggtgacgt tccagggacg cgagaatgcg
     2221 gcctatgccg cggtccatga taacgatttg ggcggcgtag gtggccatgc attcggcaat
     2281 ataatccttc cgctttgcgg aagttttttc atagccttct ggattgaatt taccggccat
     2341 gcccagtttg gcggcaggag ggacgattcc cagcagcttt tgtttttcca gtcttttgga
     2401 agcaatggca tggggggatt tgccgttgta gaacgggagc attttttcta tttccctccg
     2461 gggggcctgg aggggtgtat gcggagccgt ataagccacg taaaggaaga agggctgcct
     2521 gcttttgacg gcatcgtcaa tataggccag agccgttttg gtgatatcgt ccgtcatgta
     2581 gaaatcctcc ggcagatcct ccggacggag cagtttcccg ttttcaccga agggtctgtc
     2641 cggcatgggc ctgtagtaat ctgccgctcc acccagcaaa ccgcgggaac ggtcaaagcc
     2701 gcggtcccat gggtaggatc cccgaacttt tccaagatgc catttcccgg aaagataggt
     2761 acggtatccc gcttttttca gaagttccgg cagggtgggg atacccgggt tcaggcgtcc
     2821 gcggtagccg gggcgcccgc tgtcgtcatc caggagccct acgtccacct tgtgaggttc
     2881 gccgccggtc atgatggagg cccgggaggg tgaacagcgg gaagcggtgt agagccgggt
     2941 tgccagcatc ccctgtctgg ccagggtatc cagggcagga gtgggtattt ccgaaccgta
     3001 gcagcccggg tcggaccatc ccatgtcatc agccaggata aggacgatat taggcctgtc
     3061 cgccagtgaa gggacaaggg taagcaacca gaaggctatg ctggacatga tggaggaacg
     3121 cagggtagag cacatgaaag gaatatggaa tatcatatat aataagcgga accgctattg
     3181 attcttgcag caggaggaaa aaacaggccg ttccggaaag atccgtcctg cgtatgcctg
     3241 tttttgccgg aggccttctc ctgcggctga atgtcttttc tgcgtgatga agcggtgccg
     3301 gaagaggcgg cctccctacc accataggat ggcgcaaagc agaatggcgg ctccataggc
     3361 cattcctccc aggcaaagag gcgtccacca agcaggtttg gagcatattt tattgaccat
     3421 gtcgcggtac aggtagggct tgcctatcca gagaatcgaa aggaaaatga cgatgtaaca
     3481 ccagatggga ataaggagcc tggtcactgg ctcctgaagg aaggcggcac tgagcagggg
     3541 agctgccacc atcaggccga agatgcccag cgcccgtgga aagagcaggt ttttcacctg
     3601 cgtgaccatc agcaccagaa agacggggca tgccagctgg agcagccagc ggataccttc
     3661 aaattccgcc agccccaccc gaaagcggct cagcacgccc aatccttcgg gagccaccag
     3721 cagaaagaac cagaacaggc cgccggccag cagaatctga gccagcaggt ggttgcgcgg
     3781 agacgccagc agaaaagcct gcacctgctg cctttttacc agcgcataca ggtggaccag
     3841 caacagaacg gttcccagga tataccccgc cacagacagt ggtatcattc catcgtacgg
     3901 atgcaggaag ggagcatgct ggttcatctg gacgctaacc ttaatgatta tcccggtttc
     3961 gtaaaggcga atcagtggga gtaggaagaa aattccactg gattgccggc aatggtccgg
     4021 cagcttcccg cccgcgccgg gcggagaaac aaggggttca ggaaccttgg gtgactttgt
     4081 taagcgtagg gtcctcaacg ggggatattt tcccgtccgc ataaagcacc atgattcctc
     4141 cctggtggcg ttcaagggcc gggactacga tttcctcaaa atcctgttcc gtgatgacgc
     4201 tgcgcccgtc aatcgggatg ccgtcgctga tccagggttt gccccgcagg gcctggccgc
     4261 gcgccacatt cataggacgc cccttccggt tgtccagcca gctggtgtag gaaatggtgt
     4321 ccggcccggc gggaaggttg gcatcctccg ctccgaggat ttccggacgc ttgtcgtttt
     4381 tgcagcggaa cgtgtccggg agcatgttgg gttccttggc gttccgggcg atcaggtcgg
     4441 gttgttccgc attaacgatg ggcgccaggg cgtcccacca gttccggcat tccctggcgc
     4501 tcaggaggct gtctttcatg ccgctggagg gcagttttcc gccgttctgg cctgcgaatt
     4561 ccttgcccca gtcgctaatg cggcgcaggt tgtccgtaca gcgcgttctg gctgccgttt
     4621 ccgtggcatg ggaataaatg ctgtaggaga cgcctgccag cagggcaatg atggcgatga
     4681 ctacgagaac ttcaatgagt gtgaaacctt tgctgcgtgg ggtaagtaag cgggttttca
     4741 tgatttcccg ccattatgca cgatttaggc tgcttgaaaa ggcagaaact gcgctcttgg
     4801 gtgaaaagag cgaggccggt gctccggaga tgaaaaaggc cggtcccgtg aaggaccggc
     4861 ctgtagaaag ggtggagccg gattccactt atttggcgga agttaccaga gcagcgaagg
     4921 attccggctt cagggaagcg ccgccaacca gagccccgtc aatgtccggc tgggccatga
     4981 gttcctccac gttttccggt tttacggagc cgccgtactg gatgcgcacc ttggcggcgg
     5041 cgtctgctcc gaagacgtcg gaaataacct tgcggataaa ggaatgggct tcctgggctt
     5101 ccttggaagt tgcggtcagg ccggtgccaa tggcccagac gggttcatag gcgatgacca
     5161 ggttggatac ctcctccggg gtgagatcct tgaggccgcc cttgagctgg atttccagca
     5221 cgggttccaa aatgcccccc aggcgttcgt ccttcgtttc tccgatgcag aaaatggggg
     5281 tgatgcctgc ggcgatggcc ttcttgattt tggagttgat aatggcatcc gtttctccga
     5341 aatattggcg gcgttcgctg tggccgagca ccacatactt gaggttcagt tcattcagca
     5401 tgctggtgga gatttcaccg gtataagctc cgttgtcgta ctgggagacg ttctgggctc
     5461 cggcggtgat gcaggagcaa ccgcccagag cgttctgcac ggagggaatg gtggtgaagg
     5521 gagggatgat gaccacgtca cacgcgacgt ctttcccctt aatgaggttt ttgaagcttt
     5581 cgatgaactg ggcgccttcg gcggggccga tgttcatctt ccagttggcg gcaatgatgg
     5641 gtttgcggga cataatggaa tgatattaat caggaacacg cattgttgac aaattgaagc
     5701 tgaggtcaag cacagatcaa aagggccgca tacgcgccgt ccgatttttc ctcgtgggga
     5761 aggataagtt tttcttcttt cagcgtccat tccggatggt tctgcaaaaa gtccctgacc
     5821 agcagtccgt cttcctccgc gtcaatggaa caggtggaat aaaccagtct gccgcccggt
     5881 ttgacggcgc gggaggcatt ttccaggatg gtcttctgga gtgcggccag gcgacggatt
     5941 tcctccggag tcaggcgcca gcgcacgtcc acgcggcgtt ggatgactcc ggtgttggaa
     6001 cagggaacgt ccagaagcac ggcgtcaaag cggcccttcc attccgtgcg gcagggaagg
     6061 gaccagtccg cctgcgccgt cctgacgaag ggggatccct gcctgtccag gttttccttc
     6121 aggatgggca gccggtgttt gtggagatcc gtggcggtca ggcgggcttt gcctccggta
     6181 gcggcgatga tggctgcgga tttgccgccg ggggcggcgc aggcgtccag aatctcctct
     6241 ccgggctggg gggctagcaa atcaatggaa taacgggtgg aagggtccgc cacgtaaagg
     6301 gagcctgttt taatttcctc cagcggcaga aggccttcca cggagaacca gccgggcgcg
     6361 cggtccagcg gtgcgaggga ggccggaata tttttcatgg gtatcaaagg attggcgcgc
     6421 acatacaggc gcggtgtggt attgttccag cggagcaggt cgcgggtcat ttgcggcccc
     6481 atttgttccg tccatctctg taccagccat gcgggggtgg aataatgaat ggaaagcgga
     6541 agtttttccc ggtcctccag aatggttttc tcccgccgca aagcgttgcg caggatagcg
     6601 tttaccagtc ctctcaggcg tacggacgcg agattcacgg tttcatacac agcggcgtgg
     6661 tccgccatgc ccagcaggaa aagctggctc aaccccagtt ggagaataag acgcgcttcc
     6721 acgtccagct tgcctttccg gagggttccg atgacgtggt caagccaggt ctggttgcgc
     6781 aatacgctga agaccaaagc ctgcaccaga tgcctgtctg ccggtggaag cgcagctcgt
     6841 gaacattccc ggtccacgag ggtttccgca aaggagcggc ctgcatgcca gctcctcagg
     6901 caattcagtg ctgtctggcg gggggagggg ggattgttct tcaccgggga actctacatg
     6961 cggaacgttc aggtttcaac ctgcaaaaac gcccggcgga aaaaggggga cgccggatcg
     7021 gttccggttt tcacctgtct tttctggggc tgcccacttc cttcctgtaa atggcccgga
     7081 gtttttccag ctggtctttc cttgttcccg caagattatt cctttcttca ggatccctgc
     7141 ctatgtgaaa gaggaacggt ttattttgtt tttgcgtaag cttccagccg tctcccgtga
     7201 tgacggtgtg atccacgact acataatcgc gctgcctgga ggagttgccg gacagtatat
     7261 ccatatagga aacggcatct ttgccttccg gcatgcggat ccccgcaaga tcggcgaaag
     7321 aggccatatg gtcgtaattg gccaccaggc tgtggcagac ctttccatgg ggcagcgtgc
     7381 cgggccagga aatgataagg ggaacgcgga tgcccccttc atggctggtc catttcgggc
     7441 cggccagatt ggccatggcg ttgtttggcc caacgcggcc gcggctgccg cggaaaacat
     7501 ccagcagttc cccggtgccg tctaggacgc ctccgtggca gtcgggcccg cggccgcggc
     7561 ctttatccgt acggtagtaa agttcatgcc cgttgtcgga actgaaaatg ataatggtgc
     7621 gcttgtccag gcccagcttg cgcacctggt ccacgatggc tccgacctgc ctgtccagct
     7681 tgtccaccat ggaagcgtat tcttctgccg ctccggagca ttccctgttg gttccgctgg
     7741 cattgccata ggcctggcgg atggcagggg agcccgcgta cgtgttttct tccggaggaa
     7801 tatctaccgg gccgtgggga aggttggtgg aaaaaaagat aaacatgggc ctgttccggt
     7861 tttcctccat gaaacgaagg gtttccgcca gcatcacgtc cggagcgtag gtgactttgc
     7921 cttccctgtt gcctcttcgt ttttctgtgg ctcccggact gtagtcttcc ggagttttgc
     7981 ctccgtcggc atgggtgttg cctttcaggg ggaggcgttc gccgtctttc cagaggaagg
     8041 agggataaaa gccatgggcc ctctgatggt ccatgtaccc tacatagcgg tcccagccgt
     8101 gtcttttcaa ttctccgtgc catgtggtga atccccattc cagcttgccg aactgtcccg
     8161 tgacgtatcc ggcctttttc agcaattccg ggaggaacac ttctccctcg gcggccttga
     8221 tgcgtactgc ccgtgccgct ttctcttcca gttgctcatt ggaccagcct tcccgttccg
     8281 ccgtgatgac cagggctccc gctgtctggg tgtaggaatg ggagtggctg tcgtgcacgc
     8341 ccataagcag ggaagcgcgc gccgggcagc aatattggct gctgtagcac cgggtgaaca
     8401 tgatgccctg gctggccagg cggtcgatat tgggtgtttt cacgatttcc tggccatagc
     8461 agcccagcat gcccatgccc aggtcatccg cataaataaa aatgatattg ggtttgaagg
     8521 ccgcggcgga tgccaaaaac ggagagagcg aaaatgcagc cgccagaaat gcgtgtgaaa
     8581 gaaatcccat aacggtcact acgcaggaaa agaaaaggtt attgcaaaga aaaaaccgtc
     8641 cggaatacct tttccggacg gtttgataaa gagtggtacc gttacgggcg cttaattgcc
     8701 gtagggtttg ttgacacggc tgaattgctt caggcgcaat ccgttgaggt ggatgaaccc
     8761 ttccgcgtcg ttctggttgt acagttcctc gtggccgcct tccatcgtgg cgatttcttc
     8821 ggaatacagg gactgcgggg aacggcgtcc ggcgtacatg acgttgccct tgtaaagctt
     8881 caggcgtact tccccgttga cggtttcctg ggatttggtt accagggcct ggatggcctc
     8941 gcgttccggt gcaaaccaga agccgttgta aaccagcgta gcgtattccg ggatgaggga
     9001 gtcgcgcacc ttttgcactt cccggtcaat tgtaattgtt tcaatatcgc ggtgggccgc
     9061 cagcaggatg gtgccgccgg gagtttcata aatgccgcgg ctcttcatgc ccacaaagcg
     9121 gttttctacg atatccacgc ggccgatgcc atgcttgccg cccagggcgt tcagcacgta
     9181 catgacgccc agggggctga gcagggtata cccgtcgcgt tctcctttcg gggtgacgcc
     9241 cagttctttc agcagaacgt cgagaccatc gtactggatg ccgatgacat tgcccttttc
     9301 atacaggagc tggatgtatt cggcctggtc cggagcgtct tccggggaaa cggaaagctt
     9361 gtacatttca cggtcggcgg gggtggtgcc gtcgtaccag gtatcttcca gcatcccggc
     9421 ttcaaaggaa atgtgaagca ggttgcggtc catggaataa ggctttttca gagactgccg
     9481 gatcgggatg ccgtgggatt ccgcgtaggc gatcatttcc gtgcggcccg ggaactgctg
     9541 gcggaattcg ggatcgcgcc acggggcgat gaccttgatg ttcggggcca gggcattgac
     9601 ggccagttca aagcgtacct ggtcgttccc cttgccggtg gcgccgtggg cgatggcgtc
     9661 cgcgccttcc cggatggcca gatccaccat gtctttggaa atgcaggggc gggcgatgga
     9721 ggttcccagc aggtagcggc cttcgtaaag ggcattggcc tgcatcatcg ggaagatgta
     9781 gttggtggcg aagtcttcct tgaggtcgcc gatgaagcac ttggaggcgc ccgtcgccag
     9841 ggctttggct tccagtccgt ccagttcttc cgcctggcct acgtctgcgc agtaggcgat
     9901 gattttggca ttatattttt ccttgagcca cttcagaaga acggaggtgt caaggccgcc
     9961 agagtatgca actacgattt tcatgtacgt caatgattag tgtgcggcgg catgatgccc
    10021 cattcccccc ccggaggaaa ggacaaaatc atgtttcgcc gcgggctttt tcccggaacc
    10081 gccggagact gctttgatga accggcctta ccggggaaag caacgctcat ttcttccaca
    10141 gctttttccg gaatttactc gggattttta ctggaaccca ttggaaaacg gcactccgta
    10201 caaaatgctg aaaatgtcgg ccctttccgg tttccgttca tgaatccggc aatcttcggc
    10261 catcatccgg aacaggagtg aaacagccgc tttcctatat aaagatagcc atatcttgct
    10321 aaagatagcc atatcttgcg ttctttttgc atactctcac ggaaattccc agctgaagcc
    10381 tgtccggtga acggttccgc cgccagtctg cgtaaatggt gcagttcccg gtcctgtcct
    10441 ccctgatgat gaatttgatt gcgcaatgga tctgaaacag gcggtgcgtg taggcagaac
    10501 tccccatgtc ttttttcttc aatatttact gcagggacgg agataagnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn ntccctgccc gatcgccgtt ttcatgccgg aatatttcta
    10621 gaaaaatttt cagaaaatgt cctccgagcc ctgggagcgg ggtattttgc atgaacttct
    10681 ttcaaatatg tttcctgggg tgttggagat aatggttagg aagagttaaa taaattagct
    10741 tcttccatcc tgttgcgaat gttccggatg cctcctcacg cgatatgttt gtgaaagaaa
    10801 caggtattca attccatgcg caaagaccgc cggaaaggtc ggatgacatt gaaaacggct
    10861 tgaatttgat aaaatcgaaa aaaagcgctt gatttttcat ccccgtccct ctacttattc
    10921 cgccgaactt atggctaaga agagctggat cgcaagagac aagaaaaagg ctgaaaccgt
    10981 caagcgctat gcagaactgc gtgcgcaatt gaaggctgag aaggattata tcggtttaac
    11041 catgttgccc cgcaatgcaa gcccgacccg tacggtcaac cgctgccttg tttccggccg
    11101 ccgccgtgca tttatccgcc gtttcaagct ttcccgtatt tccttccgtg aactggccaa
    11161 tgccggcatg attcccggtg tgaccaagtc cagctggtaa ggacaggaaa aggttgcagc
    11221 cttataaaga ttttgacgct aggctgattt ttatttagcc tagcgttttg ctatgcccat
    11281 atacgagtat atttctgaaa accctgacga ccccggacag tcctgtccgg tgtgccgccg
    11341 cggttttgag ttgaggcgtc ccgtggaccg tgctccgctt gaaaaatgcc tggtgtgcaa
    11401 gcatcccgtg cgtaaggtca tcagccgcat taatgtgccg gaggtgacca agcctctttc
    11461 cgtttcagac gccaaggctg ccggattcac cgtgctggag cgccgggaca agggcgttta
    11521 tgaaaaactg tgatgacaaa gtgccgggca atatctctgc cttcttctcc gaatatatta
    11581 tttgaatcag aatgaatgat ccggatcctc tgagtattct tgcggccttc ggggccgctg
    11641 aagcgcccgg tgcaggcccg gcgctgggtg ggatgatgct ggcagttgcc ggctttatcc
    11701 tttttttgtt gttgaatgcg ttttttgcgg caggcgagtt cgccttgatg aaggttcgtg
    11761 aaagccagct gcacgccggg gaaggtgttc cggcccgaac ccggaaaaaa ctggcccggg
    11821 cgcggaaggc tgccaagcat cctgatcttt atttggccgc ctgccaggcg ggcattacgc
    11881 tttcttccct ggcgttggga ttcctgggaa cgttttttgt atcggaactg acagctccct
    11941 ttctggtctc cctgggattg ggaggcatgg tttccgttta cggaatcgct ctggccgtta
    12001 catttatttt ctttgcctgc tgccaggtgg tatttgggga gtttatcccc aaggctatgg
    12061 cgatgcgcca gccggacaag gccgccctgg cgacggttcc cctgctgtat ttcttttata
    12121 cggtgttcag atatacgggt attcttggcc tgacgggcgg aatggcgcgg tttgtgctga
    12181 aatacctgct gggcatagac ccccgttcca cggcgtgcac agtgcacagc acggatgaat
    12241 tgatgtatct ggtggaagaa agcgaacgtt cccgcgagct gacgaagcag gaggctgaaa
    12301 tttccaaaaa tgcccttgaa ctgaacgata tgtgcgtcaa ggacgtaatg accccgcgct
    12361 ctgaagtgga tgtgatggat ttgacggctc cctttgagga aaactgggag cttgcccgga
    12421 aatcccgcca cacccggttt ccgctggtgg agggagacca cctggatgaa gtgaaaggct
    12481 gggtgcatgt caaagatctg ctcaaactgg taggacggga aaatccggat ctgaggagcg
    12541 tgcggcgtga attgcgcgtg gtgccggata cgatgcccct ggacagcctt ctcacgttct
    12601 ttctgaaaga acatgcccac tttgccctgg tagtggatga attcggtgat tctatcggcc
    12661 tggtattcct ggatgatgtg ctggaacaga ttgtggggga tgacattcag gacgagtttg
    12721 accaggagga aatgcgggag tttgtgaaaa ccggcaagga tacatatgcc gtcaatgggg
    12781 ctattaccct gtttgacctg gcagattacc tgcctgaaat ggatttggat tgtccgggcg
    12841 ttactacgct gggcggttac gtaatcagcc ggatgggtta tattccggaa gaaggggagg
    12901 aattgcggat tggccgctac cgggctgtgg tgacggggtc tgacggcagg agaatcacgc
    12961 agattctgct ggcccgcctt ccggaggaac aggaggagga atagcgccat gaaggaaaaa
    13021 ccgcatccgg atatgagttt tgagatggag gcccgggcct ccggttttct ttgcgtggct
    13081 ggagtggatg aggccgggcg cgggccgttg gccggtcccg tggtagccgg cgccgttatt
    13141 ttgcctgttc tggcggagga gttaaccggt ttgaacgatt ccaagcagtt gaccgcagca
    13201 aaaagggaac gcctgtttct ggctcttttg gagtgtgaac aggcggtgtg ttccgtagga
    13261 gtggcttccg tggaagaaat agaccggttc aacattttga gggccactca tctggcgatg
    13321 gcgcgtgcgg tggaaggtct ggccctgcgt gcagatttct gcctggtgga tggattgcct
    13381 gtcaaggggc ttcctgtgcc gcaccgcgcc attgtaaagg gggacggcag gagcctttcc
    13441 atagccgccg ccagcgttct ggccaaggtg acgagggacc gcatgatgat ggaagcggac
    13501 gcctcctacc cgcagtatgg ttttgcaaaa cacaaaggat atggcacaaa agcccacatg
    13561 gaagctctgc ggcggcatgg gccctgtccg ctccatcgcc gctcgtttgc gccggtttca
    13621 caaatggaac tgtccatccc tgagcagggg taagcgggat acggcatggc tgggaatgta
    13681 cggggaactg gctgcggcct ccttcctgcg tgcggaaggg tgcgttatcc tgaggagaaa
    13741 ctggcgtccc gtcagaggcg gagagctgga cattgtgtgc cgggaagggg agtgtctggt
    13801 ttttgtggag gtgaaaaccc gtacggggaa tggttatgga ggtgcccgcc gcgctgtgaa
    13861 tgcccgtaaa agggcgctga tacgccgggg agcggcggaa tggcttcgcc tgcttccgga
    13921 accggtgaat tcccgttacg atgttgtgga agtcctgtac agggaaggga tgcctccgga
    13981 attcaggcat atccgcggag cattcggggc gaaggatttg cccgcttcat gaaaagacgg
    14041 cagggaaagg cgggaaggaa acgcggcgga tacggagctc aggtcgcgaa gccctgccgc
    14101 agcggacggg acaatctctt tagccccgca tttccccatg cggatgcaga tgtttttcta
    14161 ttataatata gcgggagtgg aaactcagtc cgcccggaga gccagaatgg cctccatgaa
    14221 atcctgggga atatccatgc gggcaagttc gtcttggaga aggacgccat tggctcctgc
    14281 gtccagcatg cggcgcgcgt cttccacggt gcagatgccc cctgcggaca ggacagtgac
    14341 agaggcgggt atttcttcaa tgagtttctc cgtgactgca ggattggctt ggagagtgtg
    14401 cgggtctgca ttgttgatgc agatgaattc cgcttccagg tccatgacgg tttcaagctc
    14461 cttcagggta tggacctcca tcatcacgtc caatcccagc ccggaagcca tgcggtacag
    14521 ctccgacaaa tcctttgccg gcagggcggc cgtcaccaga ttgacggcat ccgcccccga
    14581 gacgatggcc tggcagatca tggcgggatg aatgaacacg tcgcgcgcca tgacgggaat
    14641 ggaactgatt ctggaaacag tggaaaccat gtcccatgac ccgccgtata cttccggttc
    14701 cacagcgatg gaaatgccct gcgctccgcc ctggaggaac atgccgtaca tgtgccggat
    14761 atcctgggct gcttcccgga ggcggagggc gggatgtacc ctggatatct ccggaattac
    14821 ggagaggcgg gtttccccaa ggtccgccgc ggtgcggaat cccgcgtaat cattccgctg
    14881 gatggcggag gcccgtaatt ttccttccag aggaagaagt tttttcaaat cggcgcgttt
    14941 gcgctccaga atactgttta aataatccat atattacttt ttgggcagga agtaggaacg
    15001 ttgtttttcg gagatgggca gacctgcctg ttccatgaca cacagcatat cctcccaaac
    15061 ctttcgcttt tctgaaatat cttccgtgcg gagaagataa ctgggattat aagtaacgcg
    15121 cacgggcgtg ttgaaagctg tgtggaaggt gcctctcagg gcggaaagag gcgtctctcc
    15181 tgactggagg atgccgcggg cggagattgc tcccagggct accactactt tcgggcgcac
    15241 cagcataatt tcctcccgga gaatgggcag ggaaatttct atctcccggt ctgtaggcgg
    15301 gcggttattg gtaagctgcc gggggagggc cgggcggtat ttgaccagat gggtcagata
    15361 aatatcggaa cgggaaagcc ccatggcttt cagcatggcg tccagctttt gtccggaagg
    15421 cccggccagg ggaaccccct gtttttcttc atacaggccg ggggcgtccg ttaccatcat
    15481 gatgtccgcc ttgggattgc cggaggaaaa cacgggcgtt tccctcaagg aatccatgtt
    15541 ccggagcggc ggccagttaa cgaccagctc ccgcagggag gacagttttt cttcctccgt
    15601 ttcaccttcc agatcaaagg aaatatggcg gggtaccgga gcatcctcct ccgttctgtg
    15661 cggctgtact ccgttgaaga tatcccggag ttcattgccg aaggatgctt catccacgga
    15721 atctggaggc gtctgctccg gggtgggctg tttcggccgt gtttcatttg cggcaggctt
    15781 ggcggaaacg gaagcgggga gggggcttcc ggatatccgg cgcgcgtcca tgacccattt
    15841 tctcaggacc attctggctt cctccgtgac ggtggttttc tccacgcccc gggatagcag
    15901 ggcgcgcaga taatccagcg tgaggtgttg gggaaggccg gcggacatgt tcgttccgga
    15961 gtgtaatcta tttccggatg gatgcaacag cccattcggt atgtgcctgt ctgccgggtg
    16021 aaacgccggg gactcttatg gagggccgcc cggaagggaa ggggaaagaa gaaaattcca
    16081 taagagctcc cttcccgcgt tagatgccgt taaacggagg ggaaagtttt tttcccggct
    16141 ggatacgctt ttctggtatg agcgttatcc agaatttaga agacagcggc aaggggaagg
    16201 tttttttcaa ccgggctgga gcgtcttttc ccgtggccgt ttttgctgcg ggaatattac
    16261 ctgataacat ccgtcctttc ccgctctccc ttacaaagga agcttctccg gtcttttctt
    16321 gaccccttca ttctgctgta tgggaaggct gtgaaaatgc agatatggca agggggcgat
    16381 taggattcgc gtggaacctt gaacggcaga gggaaatccg ttcnnnnnnn nnnnnnnnnn
    16441 nnnnnnnnnn nnnnnnnnnn gagacgcgtt tttcttgctc atgacgcctt tgcggagtac
    16501 agcccctgac acttttttca gggaatattt ttgagggatt gtgtattttt catgcgtgta
    16561 aattttttat tataaatgaa atatgataaa aataaaaaag tgtgtcgtgt tatgatagaa
    16621 aaaagtattt cgggaagtat attatttgtc atgatatgac aaaaatgcgg catgttttat
    16681 atattgttta tttttagtaa tttatcttaa taggttttcc ttgtttggga gggggtgggc
    16741 ggaaaaaaag gattgccaag gcagggatta tttcgtaccc aagagccgcc gttgccgatt
    16801 ttccggcttt cgcacatggc ctgaaggttt tgccggagtg actgccatgt gtatggcgcc
    16861 taacctcgta ccgcatgaat ttaagaataa ttgccaccct gtcttgtgcc gtcgcggcca
    16921 tgtttgtcag cagctgttcg acaaacggag ccgtaaccgg catggggaaa tccagtagtg
    16981 acgctcttgt tttggcccgt gtaggtgaaa ctgcccaggg attcgtccag cccctggcgc
    17041 ccagtcgttc ggcagcgcct tcttccaaac ttcccaagtt ggggcgcgac aagcacaaga
    17101 tgccgtttta tcatcccgcc cagcgcaccc gcgtagtgcg caccacggct tatacccatt
    17161 ccgagcgcga ccatttggcc tacggtcccc ggaatgccgt cggtacggcg ctgaaataca
    17221 cgtcttccgt ccgcagcgcc gctgcagact ggtccgttta tcctttgggg actactttcc
    17281 gcatcaaggg ccagccctac ctttacgttg ttgatgatta cggaagcgcc ctggtcggca
    17341 ccggaaccat tgatatttac cagcccaaca aaaagctgat gaaggaatgg ggccgccgtt
    17401 atgtggaact gactatcgtc cgctggggag atcccgccaa cagtctggag gtgctcggca
    17461 gccgtcgcgg ctacaggcac tgccgcgcca tgtatgcggc cctgcagcac cgcgtttcca
    17521 aggggtttta tgccaaggcc gactgaccca tgttttccat cccggcgccg ccatcgctgc
    17581 atggcggcgc ttttttattt tccgccgtgc gggcggagtg gtaatctggc ggcatgaata
    17641 cggaaaaacg ggcttccatt gtccaggagg aactcatgtc cctgtacggg gctcctccca
    17701 ttcccctggt gcaccgcgac gcttacacgc tgctggtggc ggttctcttg tctgcccagt
    17761 gtacggacaa gcgggtgaat ctggtgaccc ctgctctgtt tgccctggcc tccaccccgg
    17821 aggaaatggc ccggcaggat gtggaagcgg tccgggaaat tgtgaggccc tgcggcctct
    17881 cggaaaggaa ggcatccgcc atcgtgaacc tcagccgcat cctggtggaa aaatatgagg
    17941 gaaaggttcc ttgcgacttt gctgctctgg agtctcttcc gggggtgggg cataaaacgg
    18001 cttccgtcgt catggttcag gcctttggcg ttcccgcctt tccggtggat acccacattt
    18061 tccgcctttc acggctgtgg gggctgagca cgggaaaaac ggtggaaacc gtggaacgcg
    18121 atttgaaaag ccttttccct gaaaaatcat ggggagatct tcatctgcgt attgtcttgt
    18181 acggccgtga atattgtcct gcgcggggat gcggggggcg ctgccccatc tgcagccggc
    18241 tggcccggga gagcggctgc gaaggcgtct gaatttttta cagggtctaa aatgatgctt
    18301 gcaagtctga atttgcatgc tttaatacac cagtatcttt tcgaacaacc atataactta
    18361 aattatgaag aaccttttta gactgggttt ccgtttcgtt gcatttgctt ccgtcctgat
    18421 ggctgcgttt tctacatccg ctttcgctca ggaagccgcc gaagccgttg ccccgcagga
    18481 acagaccatg cttgacaaat ggatcatcgc cggtggttgg accatgattc cgattatgct
    18541 ggtggaagct ttcatcattt tcctggtgat ttacaacatg gtagccctga aaaaggaaaa
    18601 attctgcccg gaagatctca aggtcactct gttgcagctg atggccgaat gccgcatccg
    18661 gtccgccatt gaagttgcgg ccggcagtcc cacctatttg ggccgtctgg ttgcctacgc
    18721 cctgccgaac gtggacgcta cccgtccgga agacctcggc aaagatgcta ttgaagacgc
    18781 cgtggccgac tttacggcca atgaaagccg ttccgtgttc aaatggatca acatgctggc
    18841 cctttgcgcg cagatttctc ccatgctcgg cctgttcggg acggtgcagg ggatggtggg
    18901 cgcgttcggg acgctggcta ccgccggcca ggcggacccc acccagctgg ccggtgacat
    18961 ttccgtggct cttctgacaa cgttctgggg attgatcaac gccattatcg ccactccgtt
    19021 cttcttcttc cagaagggca ttgccaacgc ccagattgcc gaatgtgtgg gcactgtgca
    19081 ggaaatggtg aacacctcca tcaacgtggt gaatgccgaa gcccagcttg cccgcatccc
    19141 cgaaggcctg gcctgattgc cggaacaggc ggtccggccc agaagcgtcc gctcagtcgg
    19201 aaccgggccg gagaacctca acaaaatgta gcttatgggt aagaaaaaag ctaatgtagc
    19261 gcctgaagaa gaaaatgggg aaatggatat gtcccccatg atcgacatgg ttttcctcct
    19321 gttgattttc ttcgtggtga acgctacggc cattaccgtt aaaaaggata agaacattca
    19381 gatgcccacg gccagcagct ccggtgaagt gaaatccgcc aacggctgca ttgtggtgaa
    19441 tgtttatggg gaaggtgcgg gtaagcggcc gcccggcgta gatcccagcg ttctctggtc
    19501 ctccgacgta ggcactccgc tcaattcctc cga
//
LOCUS       DAOMSU010000009        18748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_86_length_18748_cov_2.936179, whole genome shotgun sequence.
ACCESSION   DAOMSU010000009 DAOMSU010000000
VERSION     DAOMSU010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18748
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86_length_18748_cov_2.936179"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            506..2038
                     /locus_tag="PK077_00920"
     CDS             506..2038
                     /locus_tag="PK077_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22894.1"
                     /translation="MPSSAEGSMVNCSKCGHEFLCDREKLIPKKPFAKAKKAVSVHSP
                     KDRREAIQSALVETGLIQLVCLCASLAVAAGCVWIFYNASSEMDGTFFLSMLQQPQQK
                     AFALFFACLAGGLMACAGRRHKIVFILLGLLVAGGIASLPFVYPVKVNPSLLGGHEEG
                     SAGEEDVSGFTSMGLEQLPSKNASVQNYGDGDLKPLFSAIDRKESQGVLGVWVVGVNA
                     ANRDMVKAYLKRMTQSEDEPIFYDRKGTGGGLFVITPTPITFKEFVEVAGKMGKVSLQ
                     DKERFFVEVILNRDRFEARPASAALQDERHQYFVLANLKELSCLDIRRVIAAAKRLSA
                     VNPDKMRGEVSSRLVELLREPWGRDAEYVTALASALVVWAEPDHEEAQRVVDYVATEL
                     KKADCEIPASLIRFLLHGSEKRSLALLLEEWKKDPQKWEDECKAAGPTGEADIIRSLN
                     ESDDFVLKRSAARILGEIGSEASLSALKAFTHDADNELRLCAELSVNLIEKRLGIDSS
                     AR"
     gene            2367..4328
                     /locus_tag="PK077_00925"
     CDS             2367..4328
                     /locus_tag="PK077_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN22895.1"
                     /translation="MTDQENMKAAVPDAARRYMRYLLIMAGLGGLLYGVDVGVIAAAL
                     PYIEQTAGFNPSQLSQVVAAVLFGSVLSSLFAGYLADKMGRKALITVAAALFTASIPV
                     ICLSQEVFGIMLLGRILQGASAGIVGVVVPLYLAECLSAESRGKGTGMFQFLLTVGLV
                     FAAVVGLLAASYVGGVENSGASEESLTSAKVLAWQAIFWVCAIPGLFLFFGSFRLSES
                     PRYLFRRGRKDEAMAVLVRSYGDARAKEVFDEMVHIEEEEKQKAEELKKQSSSGESLL
                     QRKYIYPFVLAVLVLAFTQATGINSVLNYSVKVFQQAGLEGTTANWADFTIKVVNCLM
                     TIVAMVLVDRKGRKFLLKIGTAGIVVGLLGTGFLFNNVEKARKDVTADVAALLAAQSP
                     SVQKEFEQGKDVGSIRTLQLERTPDSPFIRNLLAKNGMADKDINRMQLIITYDQPEAN
                     PAWYQFLMGSSTQLSVVEFSELTKDTKDIKKEEDKASLAVIKAVPDSTNKMVVNGKDG
                     YAMKPVSILKAELGEKPDTSMGWGVTAFFIIFIAFYATGPGVCVWLALSELMPARIRS
                     NGMAIALLINQLVSTVIAGSFLPWVGSCGYSGVFFTLGGITVLYFITVTFFLPETKGR
                     SLEEIEGYFTTGKMPEDPKMIGEGIEAEE"
     gene            complement(4547..5605)
                     /locus_tag="PK077_00930"
     CDS             complement(4547..5605)
                     /locus_tag="PK077_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22896.1"
                     /translation="MRQMTGTMLLLSSAVVMAAPWAHAEEKTIQLTEAEQQEIKTANE
                     KLLGLTLRFLHDSWPLEIMFPGEVQEEFHSILQCHQMLEQFRQTGILLLQTPDRTTPL
                     HLCIALGLNRLAVRMVEAGAPVNAQSIFMHDGTKEPGDTPLTWACLSGLYMNSTAEER
                     LPLVHALLKHGADPDQPGPWGVTPFMYSAALNDSDPGQEKIALALLDAGSPDLKRRMN
                     AQARGVGFLSLSPAIYERLIKAGCDVNERFFESKQSPLHLVCTKEKPAERLIPLIELL
                     INAGADPNQPDVDGLTPLMACNSPEIAVCLMNHGANPSLRNDDGQTAYDFHMKNGYPP
                     IAEAIKHWQSKQKKEEAR"
     gene            complement(5658..6938)
                     /gene="hemL"
                     /locus_tag="PK077_00935"
     CDS             complement(5658..6938)
                     /gene="hemL"
                     /locus_tag="PK077_00935"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HRN22897.1"
                     /translation="MNQSAQLFSRARSVIPGGVNSPVRAFRNVDGDPFFVQSAKGAYI
                     TDADGRQLIDYIGTWGPAILGHAPQPVLDAVHAAVDRGLGYGIPAPAEVDMAEMITDM
                     VPSVEKVRMVNSGTEATMSAIRLARGYTGRRKIIKFIGCYHGHVDSLLVAAGSGALTF
                     GEPDSAGVPREMTQLTLTVPYNDREAVKKAFELHGSDIAAVILEPFPANAGLYFPQND
                     FLHFLREITLRHDTLLIFDEVMTGFRVAPGGVQQLYGITPDLTCMGKVIGGGLPVGAF
                     GGRSEIMDCLSPLGPVYQAGTLSGNPVAMAAGLAQLRELLKGNAYERLEQLGARMEEG
                     IREALKKHGRNYTFHRASSMFCLFFTEEEVYNLESAQKASKKLFKSFFWNMLEQGVYF
                     APSPYETGFISTAHTEEDIDRTVEAVRISLSRLG"
     gene            complement(7023..7274)
                     /gene="rpsU"
                     /locus_tag="PK077_00940"
     CDS             complement(7023..7274)
                     /gene="rpsU"
                     /locus_tag="PK077_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRN22898.1"
                     /translation="MREVTVRKGEPIDRALKRLKTKLDVEGILDEMRRRRAFETPMDE
                     RRRKARSASKRNKVKWRYSNKSEETASETAETPASAPEA"
     gene            7503..8993
                     /locus_tag="PK077_00945"
     CDS             7503..8993
                     /locus_tag="PK077_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HRN22899.1"
                     /translation="MNRLHTLIDGFSRVRILCIGDVMLDKFLYGSVSRISPEAPVPIM
                     KMDRETRMLGGAGNVVSNLCALGCRTTFISVVGDDGHGRQVRRFLEETHCVPELVERE
                     GYETTVKIRFVAGKHHLLRADQEQPLRMEPELASRFLERVDACLPEADLVLLSDYGKG
                     LFDGRTAPDVIARCRAARKPVIVDPKGADYSRYRGATLVKPNMKEFQEATGVALNPAM
                     AGWEQDAVAGAQRLFSEFGIENLIVTLSEHGMIFIPSSSPSDFVCIPTEARDVFDVSG
                     AGDTSLASLGAALAAGAAVPDALVVSNVAAGIVVGKFGTASVTGAELKKALEEKDIQT
                     PSWHHRDNILTPEAAAELAERFRREKKVVGFTNGCFDLLHLGHLHSFMKAREACDVLF
                     VGLNTDASIKRLKGEDRPINNEEMRSLLLASLDFVDYVVPFDEDTALPLIEKLRPDVI
                     AKEGYPLECWPEGQFVKSYGGQALELPRLEGFSSTNIINRMKSSPE"
     gene            8990..9592
                     /gene="gmhA"
                     /locus_tag="PK077_00950"
     CDS             8990..9592
                     /gene="gmhA"
                     /locus_tag="PK077_00950"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HRN22900.1"
                     /translation="MSEYIRNQILGIADNFKALASMAGDIEQVARICTDTLKAGNKIM
                     FCGNGGSAADSQHLAAELVGRYKLNRPAMNALALTVDTSILTAVGNDYGYETVFSRQL
                     EGVGRPGDLLVGLSTSGNSRNIVLAMELARRMGVRTVALTGRGGGEMKEVAEFCIAVP
                     SDATNNIQEMHIAVGHLVCELVEREIYGGEGFVSGQGRRG"
     gene            9549..10055
                     /locus_tag="PK077_00955"
     CDS             9549..10055
                     /locus_tag="PK077_00955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRN22901.1"
                     /translation="MAGKALFLDRDGVVNVDGGYVHRIEDFRLVPGILNLCCQAKEKG
                     YLVLVATNQSGIGRGMFSEDDFERLTEYMRGVFRSSGAEIAGVFHCPSADDGHPDRKP
                     NPGLFLKAAAAHGLDMKACVSVGDRERDIRAALAAGVGRNFLFSAEDVPTLATARVET
                     LEEVAARL"
     gene            10245..13238
                     /locus_tag="PK077_00960"
     CDS             10245..13238
                     /locus_tag="PK077_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HRN22902.1"
                     /translation="MNLILFRSMMTAAALGAGLALPVSAQSSSPAFSASPEGSRAGKT
                     AKMPVYPIPQKMTRSGGSVTVPALKLLGARDEPTVNALKSMFSLAPNGLPVQMIIIKG
                     SKKPAGNVPRKAGAYSLNVTPQGIRMTGYDDEGLFYAAQTLLQLAEKTSSGVTVPEVE
                     VLDWPDVPFRGTIEGFYGLPWGQEGRISQFKFYGKYKMNTYIYGPKDDVFHGFSKRWR
                     EPYPADMAKDLKELVKVAKQNKVNFVWAVHPGADIHWGEADRKAAVKKFEMMYDLGFR
                     SFAVFFDDIGGEGAKPEGQVEFLNYLNKEFIHKKPDVTPLIVCPTAYSGGGGRYHEVM
                     GEHLDKDIGIMWTGSSIVSDIRTPALKGINKYLKRPAFIWWNFPVTDYVRHALFLGRT
                     YGVDADAMPFMQGFASNPMDKPEASKISLFSVANMTWNAKAYDSDRTWKDSIRILFPG
                     CSSAMQTFADHNSDGGPSGHNYRKEESVEIAPVVEQVLELCRRGARVSGSKAFDRLKA
                     EFAKMAQAPAAIRAKSNNPAFVAEVEPWLIQFESLGKAGVNSMRMIEATEAGNAAGAL
                     NHAMEAACLLAEMQRYSREISKAINKHVTEVTKKNSPWQTAVKPSELVMAPAVRELLD
                     MGSTPVLSRVSGQAVGRVKPYVSTKSKIGIEKMLDDDPESFYYCKEVQKKGDFFGVDL
                     GVPREIRTVSIVMGRNDSDTDAVNRGQLEVSLDGQSWSSLMPESSGLRVEYRGNGKKS
                     RFVRYRATAQGVPGGKPDVWTAIRDFKVNAPAAPSVLTDAPAFRNAVVEAGDRDISLK
                     RIMEVHPLPPKKFLGLQIPAGASVESASVNLKTPDMKWAKLFISMDGKSWAEVPLKED
                     GSADIGGVIKGIRLLNASSSPQEVTLEEFRLNLANKGGKSADSGAAGDFNLATFLQVE
                     LSPERAEIPCDFPRAGSVIVLSDGKEASVLACGADGRWVPVGNLAKGRKVNTFSLKSV
                     KKPVKALGLTGKKGSSVNIFEVIWK"
     gene            complement(13325..13984)
                     /locus_tag="PK077_00965"
     CDS             complement(13325..13984)
                     /locus_tag="PK077_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22903.1"
                     /translation="MQSSPDQPSSGAWLGLPPDEERPVSRGARYRCIFLMLLYWAVLS
                     AIPQGADRLAEIGTNLPLWMGALELTPFLLVPAFLFLAVRTGAQWRALMITVYAGLFL
                     NLLMNMALPSGAMWGDLPAAGPHGTLVWPGGDHREWLVTLPRFPQFWALVIYVFLSGS
                     GTPPLLRLLALAWWFLLCVAPVNTGMVGYADILGPLVIIALVLGCMHLAEKRASRQTK
                     G"
     gene            14213..15259
                     /locus_tag="PK077_00970"
     CDS             14213..15259
                     /locus_tag="PK077_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRN22904.1"
                     /translation="MTRSRFLMILPALFLCLLAVSCGKKEKEDPNVVELNFGHFPNVT
                     HVQGLVAHHFSRQGKGWFEERLKEATGKDVRINWYVYNAGPSAMEAVFARSIELAYVG
                     PSPAINAFVRSRGEDIRMIAGAVEGGAALVVPKDSSLKEPADFRGKVIATPQLGNTQD
                     VSARAWFSRGGLHVTQRGGDVTILPTPNPEQLSLFRQGKLDGVWTVEPWVSRLVLTAG
                     GRVLVDEKESIATVLVCGAEFLREKPEVAKALVQAHEELNEWIRLHPDEVQSIVVREL
                     EDLTHSRIDPALIAEAWKSIVIKDRISIPKLRQFVQDAHQAGFMKEVPDVGGLVVPEA
                     AEEQLTMAKEAGGR"
     gene            15256..16044
                     /locus_tag="PK077_00975"
     CDS             15256..16044
                     /locus_tag="PK077_00975"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN22905.1"
                     /translation="MIAGAEHCGPGGCKLRIAGVSKVFDGRRGKVVALEGINLNIRGG
                     EFVCLVGASGCGKTSLLNIIAGLEFPSSGVVELDGEPVTGPGRDRTVMFQESALFPWL
                     DVLGNVMFGLKLVPGLTRGDRLAMAEKNLELVGLKECAHAHIHELSGGMKQRVALARA
                     LAASPRILLMDEPFAALDAMTREQLYQDIQEIHLRCGMTIVFVTHNMREAVCLGDRVV
                     LLTPHPGRVCEEYFVDFPHPRDINSSDLAELSARITRDLKGATA"
     gene            16041..>16596
                     /locus_tag="PK077_00980"
     CDS             16041..>16596
                     /locus_tag="PK077_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRN22906.1"
                     /translation="MNRNKWSKWGAHACSLVFFILVVWAWQHLSDLRVWKPYLFPSPL
                     EVWEYLRSSFQDGSLEEASWITMKRLVMGYGIGLVTGIPLGMLCSRFQLMQNTLGLVS
                     LGFQALPSVCWVPLATLWFGQTEGAMLFVVIMGTLWSVILATANGMRNVPPIYARAAR
                     TMGAGPIYCLIHVTLPASAPFVVSG"
     assembly_gap    16597..16606
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <16607..16830
                     /locus_tag="PK077_00985"
     CDS             <16607..16830
                     /locus_tag="PK077_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN22907.1"
                     /translation="AFAWRSLMAAEIFVPILTGFGLGQLLHFGRELNAMNQVVGIMFV
                     IVVIGLLSDKILFSPLERFLHRRWGTGQA"
     gene            complement(16890..17927)
                     /gene="lpxD"
                     /locus_tag="PK077_00990"
     CDS             complement(16890..17927)
                     /gene="lpxD"
                     /locus_tag="PK077_00990"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HRN22908.1"
                     /translation="MKLTLEAVAALTGGKILSGDPELTVFGVASLLDASREEASFLGN
                     EKYFQDFLHTSAGIVLVPPGLPAYPEHAAFVEVDNPSMAFNVLVKYFMASAYRFTPGI
                     HPTAIIDPTASFNPDKIHVGAYTCIGAHCVIGDGTDIGNGCDIGDGVTMGENCRLHAH
                     VTIRERCKLGNRVTIQPGAVIGSDGFGFLMGDNGRYVGIDQVGIVELGDDVDVGANTT
                     IDRARFGRTIVGEGTKIDNLIQLGHNVVVGRHCIIVAQSGIAGSTKVGDYATIAAQVG
                     ISGHLNIGSKSTLGAKTGVISDIPENSTYWGMPAFPYKDATRQYAALKKLPALIKEVR
                     ALKKELDSSGK"
     gene            complement(17967..18563)
                     /locus_tag="PK077_00995"
     CDS             complement(17967..18563)
                     /locus_tag="PK077_00995"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HRN22909.1"
                     /translation="MALSATANAELKVATVDVQKLFADYYKTHEAQAEVDKAAQTVQE
                     TNNTRAETIKKMEADFNDMVKRLQDPMLNEKDKKELEQKAQIKRQEVIALEQERRGFV
                     ERQLKSLQEQMKVRSAKIMGEITKITEGIATKGNYDLVLDKSAQALRSNQVFVYTKPG
                     MDITPTVMKELNKDAPEGFDPSKKKAPAVPAAPAAPAN"
BASE COUNT         3677 a         4635 c         5963 g         4463 t
ORIGIN      
        1 gcaatgacat ggcgaaaatt ctactaccaa atttcctctt ctgcaagcgt gttcactcgg
       61 aatgacgtga ggcgggaaga ggagcatgaa gagcgttttt atctttttcc aatggcccgt
      121 gttgtaaaat tcaaataatt tatatcaagg tgcgtttgga aattgagccc ctgataaggg
      181 agactgaaaa atttccagct tttcgccagg ggaagatggg aattttccct ttaccttgtt
      241 ttccgaagtt tgcggaatgg ttttcgatta aatgttattt tcaatgagag cggtttgagc
      301 gagtttttac attcagaaaa aaatcagaga gtcgaaatga aaaggagaaa tgatggaaat
      361 aggagccgat ggaagtttct tgcatatttt cgcggggtac atggtaagag gaaagttggg
      421 aagacaggta ttgcaaaaga atgaaaaacg tgcatattgg agcccggtga atctgaattg
      481 tccacagtgc aaacgacata tcaatgtgcc ttcttccgcg gagggttcta tggtgaattg
      541 cagcaaatgc gggcatgaat ttctttgcga cagggaaaaa ctgattccta agaaaccttt
      601 tgccaaagcg aagaaagccg tttccgtcca ttcccccaaa gacaggaggg aagccattca
      661 gagcgccctg gtggaaacgg ggctgattca attggtgtgc ctttgcgcgt ctctggctgt
      721 tgccgccggc tgcgtctgga ttttttacaa tgcttcttcc gagatggatg ggaccttttt
      781 cctttccatg ctgcagcagc cccagcagaa ggcgtttgcc ctgttttttg cctgtcttgc
      841 cggaggcttg atggcctgtg ctggacgcag gcacaagatt gttttcattt tgctggggtt
      901 gctggttgca ggggggattg cttccctgcc tttcgtgtat ccggtcaagg tgaatccttc
      961 cctgctcgga ggtcatgaag aggggagcgc cggggaagaa gatgtctccg gctttaccag
     1021 catggggctg gagcagctgc cctccaagaa cgcttctgtc cagaattatg gggacggtga
     1081 tttgaaacct ctgttttccg ctattgaccg caaggaatcc cagggggttc tgggcgtatg
     1141 ggtggttggg gtgaatgcag ccaaccggga tatggtaaag gcgtatttga agaggatgac
     1201 ccagtccgag gacgaaccta ttttttatga ccgcaaggga acgggcggcg gactgttcgt
     1261 tattactccc actcccatta cgtttaagga atttgtagag gttgcgggca agatggggaa
     1321 ggtgtccctg caggataagg agcgtttttt tgtggaagtg attctgaacc gggacaggtt
     1381 tgaagcgcgg cccgcttccg ccgcgttgca ggatgagcgc caccagtatt tcgtgctggc
     1441 caatttgaag gagctgtcct gtctggacat ccgtcgcgtt attgccgctg caaaacgttt
     1501 gtcggctgtg aatccggata agatgcgagg tgaggtttct tccaggctgg tggagcttct
     1561 ccgggaaccg tgggggcgtg atgcggaata tgtgaccgcg ttggcttcgg cccttgtggt
     1621 atgggcggaa ccggatcatg aggaggctca gcgcgtggtt gattacgtgg ctacggagct
     1681 gaagaaggcg gattgcgaaa ttccggcgtc tctgatcagg tttttactgc atggttcgga
     1741 aaagagaagt ttggcgttgc ttctggagga atggaaaaag gatccccaga agtgggagga
     1801 cgaatgcaag gcagccggcc caacgggaga agctgatatc atccgttcgc tgaatgagtc
     1861 ggacgatttt gttttgaaac gttccgccgc ccgcattctt ggggaaatcg gttcggaagc
     1921 ttccctgtcc gcgcttaagg ctttcactca tgacgccgat aacgaattgc gcctgtgcgc
     1981 ggagttgtct gtgaacctga ttgaaaagcg gcttgggata gattcttccg ccagatgagg
     2041 ccggtaaggg cggcttccgt ttccgtgatg gcgcatcgcg ctgtttttct ggctccaata
     2101 cggaagggga gttttttccg gacggtttac ggggattgaa gtgcggctcc ggaattttcc
     2161 catggctgtt tttcggagcc gggggatgca tgagggcttt tttgcagagg tgtccaaatg
     2221 atttcctgtt ttcggacagt ttgttacttg tttcttttgc gatattttca ccatttgaac
     2281 gcatttccgg gtaggaaatt tgtttttgcc cgggaagtgt cttttttggt tgcggagaag
     2341 agccaattta ctagaatgca acgaccatga cagaccaaga aaacatgaaa gccgccgttc
     2401 cggatgcggc caggcgatat atgcgctatc tcctcatcat ggccggtttg ggcggcctgc
     2461 tctacggtgt ggacgttggc gtgattgctg ccgcgcttcc ctacattgag cagacggccg
     2521 gttttaatcc ctcccagctt tcccaggtgg tggccgccgt gctgttcggc agcgttcttt
     2581 cttccctgtt tgcgggctat ctggccgaca agatgggacg caaggcgttg attaccgtgg
     2641 ccgcagcctt atttacggcg agcatccccg tgatctgcct gtcccaggag gtattcggca
     2701 ttatgttgct gggccgtatt cttcagggcg ccagcgccgg tattgtgggc gtggtcgttc
     2761 cgctgtatct ggccgagtgc ctgagcgccg aatcccgcgg caagggaacg gggatgttcc
     2821 agttcctgct gacggtgggc ctggtgtttg ccgccgtcgt cggtttgctt gccgccagtt
     2881 atgtgggcgg cgtcgagaat tccggcgcga gcgaagaatc gctgacttcc gccaaggttc
     2941 tggcctggca ggctattttc tgggtttgcg ccattcccgg cctgttcctg tttttcggtt
     3001 ccttccgttt gagcgaatct ccccgctacc tgttccgccg cggccgcaag gatgaagcca
     3061 tggctgtgct ggtgcgcagt tacggggatg cccgcgccaa ggaagtgttt gatgaaatgg
     3121 tccacattga agaggaagag aagcagaagg cggaggaact caaaaagcag agctcttccg
     3181 gcgagtccct tcttcaacgc aagtatatct atccctttgt tctggcggtg ctcgttctgg
     3241 cgtttacgca ggctacgggc attaattccg tgctgaatta ttccgtgaag gtattccagc
     3301 aggcaggcct ggaaggcacc accgccaact gggcagactt taccatcaag gtcgtgaact
     3361 gcttgatgac tattgtcgcc atggtgctgg tggaccgcaa gggccgcaag ttcctgctta
     3421 aaatcggaac ggccggcatc gtggtcggcc ttcttggcac cgggttcctg ttcaataatg
     3481 tggaaaaagc ccgcaaggat gtgactgcgg atgtggctgc cctgctggcc gcccagagcc
     3541 cttccgtcca gaaggagttt gaacagggca aggatgtggg ttccatccgc acgcttcaat
     3601 tggaacgtac tccggattcc cccttcatca ggaatcttct tgccaagaac ggcatggccg
     3661 acaaggacat caacaggatg cagctcatca tcacctatga ccagccggaa gccaatcccg
     3721 cctggtacca gttcctgatg gggtcttcca cccagctttc cgtcgtagag ttttccgaac
     3781 tgaccaagga taccaaggat atcaagaagg aagaggacaa ggcttctctg gccgtgatca
     3841 aggccgttcc ggattccacc aataaaatgg tggtgaacgg caaggacggt tatgccatga
     3901 agcccgtttc cattctgaag gcggaattgg gcgaaaagcc ggatacctcc atgggctggg
     3961 gcgtgactgc gttcttcatt atctttattg ctttttatgc taccggcccc ggcgtgtgcg
     4021 tctggctggc gctgtccgag ctgatgcctg cccgcatccg ctccaacggt atggcgattg
     4081 ctctgttgat caaccagctg gtttccacgg ttatcgccgg ttccttcctc ccgtgggtgg
     4141 gcagctgcgg ttattccggc gtgttcttta cgctgggcgg cattacggtg ctgtatttca
     4201 ttacggtgac cttcttcctg cctgaaacca aggggcgttc cctggaggaa attgaaggtt
     4261 acttcacaac aggcaaaatg ccggaagatc ccaagatgat tggcgaaggc atagaagcag
     4321 aggaataatt ctttcttcct gtggttttca aggccgcttc ccctgaattg gggaagcggc
     4381 ttttttataa caggaaacag ggtgggctgc ctgcatggct tcggaagcac ggctgttccg
     4441 gggagagggt tccggccacc tgttcccggg ccgtgacggt atccgctttt ctttcccgca
     4501 ccagtaggag aaggaggggg gcaaagccgg cgatggcgcc cgctatttag cgggcttcct
     4561 cttttttctg cttggactgc cagtgtttga tggcttccgc aatgggggga tacccgtttt
     4621 tcatatggaa gtcataggct gtctggccgt catcgttgcg gagagaggga ttggcgccgt
     4681 gattcatgag gcaaacggct atttccggag agttgcaagc catcagcgga gtcaggccat
     4741 ccacgtccgg ctggttggga tccgctcccg cgttgatgag aagttcaatg agaggaatga
     4801 ggcgttccgc cggtttttcc ttggtgcaga ccaggtggag gggtgattgc ttgctttcaa
     4861 aaaagcgttc gttaacatca cagccggctt tgatgagccg ttcgtaaatg gcgggggaca
     4921 ggctgaggaa gccgactccg cgcgcctgtg cgttcattct gcgcttgagg tccggggagc
     4981 ctgcgtccag gagcgccagc gctatttttt cctggcccgg atcggagtca ttgagggcgg
     5041 cggaatacat gaacggggtg acgccccaag gccccggctg atccgggtcg gcgccgtgtt
     5101 tgagcagggc gtgcaccaac ggcagccttt cttccgcggt ggaattcata tagaggcctg
     5161 agaggcacgc ccaggtaaga ggggtgtctc ccggctcttt tgtgccgtca tgcatgaaaa
     5221 tggattgagc attgacggga gcgcccgcct ctaccatccg gacggccagt cggtttaatc
     5281 ccagggcaat gcagaggtgc agaggcgtgg tacggtccgg cgtctggagc aggaggatgc
     5341 cggtttggcg gaattgctcc agcatctgat ggcattgaag aatggagtgg aattcctcct
     5401 gcacttctcc gggaaacatg atttccagag gccaggagtc atgcaggaaa cggagggtaa
     5461 ggcccagcag tttttcgttt gccgtcttga tttcctgttg ttccgcttcc gtgagctgga
     5521 ttgtcttttc ctccgcatgc gcccaaggag cggccataac gactgctgag gacagaagca
     5581 gcatggttcc tgtcatttgt ctcatgaagt ccttataggg gaagggtcgt gagctgtcac
     5641 ttcaaatcag gggcatgtca acccagcctg gagagactga tgcggacggc ttccaccgtg
     5701 cggtcaatat cttcttccgt atgggctgta gagataaagc ccgtttcgta cggggagggc
     5761 gcgaagtaga cgccttgttc cagcatgttc cagaagaagg atttgaagag ttttttggaa
     5821 gctttttgcg cggattccag attatagact tcctcttccg tgaagaagag gcagaacatg
     5881 gagctcgcac ggtggaaggt gtaatttctg ccgtgttttt tgagggcttc gcggatgcct
     5941 tcttccatgc gcgctccgag ctgttccagg cgttcatagg cgttcccttt caggagttcc
     6001 cggagctggg ccaatcccgc cgccatggcg accggattgc cggacagggt tcccgcctga
     6061 tacacagggc caagcggaga gaggcagtcc atgatttccg agcggccgcc aaaagctccc
     6121 acggggaggc ctccgccgat gactttgccc atgcaggtaa ggtctggagt gatgccgtaa
     6181 agctgctgca cgcctccggg agcgacccgg aagcccgtca tgacttcgtc aaagatgaga
     6241 agggtgtcgt ggcggagcgt tatttcccgc aggaagtgaa ggaagtcgtt ttgcggaaag
     6301 tagagtccgg cattggcagg aaagggttcc aggatgacgg cggcaatgtc gcttccatgg
     6361 agttcaaagg ctttttttac tgcttcccgg tcattgtagg gcacggtgag ggtgagctgc
     6421 gtcatttccc tgggcacgcc cgcgctgtcc ggttctccga aggtgagcgc tccggaaccc
     6481 gccgctacca gaagggagtc cacatggccg tggtagcagc cgatgaattt gatgattttg
     6541 cggcgtccgg tatagcctcg agccagcctg atggcggaca tggttgcttc cgttccggag
     6601 ttgaccatgc gcactttttc cacggaggga accatgtccg tgatcatttc cgccatgtcc
     6661 acttccgccg gcgcgggaat tccatagccc aaccccctgt ccacggcggc atgtactgcg
     6721 tccagaacgg gttgcggagc gtggcccaga atggccgggc cccatgtgcc tatgtagtca
     6781 ataagctggc gcccgtccgc atccgtgatg taggctcctt tggcggattg aacaaagaag
     6841 gggtccccgt ccacattgcg gaaggccctg acgggggagt tgactccgcc ggggataacg
     6901 gagcgcgcgc gggagaagag ctgggctgac tgattcatgt gcagagggaa gcaaaaaggg
     6961 caatccttga gaaagggttg ccctcatgga aattgggttc ttattcccga actaatactg
     7021 ttttaagctt caggagcgga agcgggtgtt tcggcggttt cggaagcggt ttcttcgctc
     7081 ttgttgctgt aacgccattt taccttgttg cgcttgctgg cggaacgagc cttgcggcgg
     7141 cgttcgtcca tcggggtttc aaaggcgcgg cggcggcgca tttcatccag aatgccttca
     7201 acatccagtt ttgttttgag gcgcttgaga gcgcggtcga taggttcacc ttttcttacg
     7261 gtaacttcac gcatgatttg ttttcctttt tctattcgtc cgggaacggg agacaatagg
     7321 tagtgctttc ctgcttacag gtcaagattt ttatcatgac tgacctattt tttgccactg
     7381 aatgagagtt ctgccgtccg gaacggcggg aagcggccgc gttgccccgt tttccggttc
     7441 cggagcgcgg ttcaactgtc cttgccttat aacaaggggc gtggtagaaa gaggcgtctc
     7501 ccatgaaccg gctgcataca ttgattgacg gattttcccg ggtgcggatt ctctgcatcg
     7561 gggatgtaat gctggataag ttcctgtatg ggagcgtcag ccgtatttcg ccggaggctc
     7621 cggttcccat catgaagatg gaccgggaga cgcgcatgct gggaggcgcg ggcaatgtcg
     7681 tgtccaatct gtgcgcgctg ggatgccgca ccacttttat cagcgtggtc ggggatgatg
     7741 gccatgggcg gcaggtaagg cgttttctgg aagagacgca ctgcgttccg gagctggtgg
     7801 agcgcgaggg gtatgagacg accgtgaaga ttcgttttgt ggcgggcaag caccatttgc
     7861 tgagagcgga ccaggaacag ccgctgagga tggaaccgga gctggcttcc cgttttttgg
     7921 aacgagtgga tgcctgtttg ccggaggcgg atcttgtcct tttgtccgat tacgggaaag
     7981 gcctgtttga cggacgaacc gccccggatg tgattgcccg gtgccgtgcg gcgcgcaagc
     8041 ccgtcattgt ggacccgaag ggagcggatt attcccgcta taggggggcc acgctggtga
     8101 aacccaatat gaaggagttt caggaggcta ccggcgtggc gctcaatcct gccatggccg
     8161 ggtgggaaca ggatgccgtg gcgggggcgc agaggctgtt ttccgaattc ggcattgaga
     8221 atctgattgt cactttgagc gaacacggca tgattttcat tccttcctcc agcccttccg
     8281 attttgtctg cattcctacg gaggcccggg atgtttttga cgtgtccgga gcaggtgata
     8341 cctccctcgc cagtctgggc gcggcactgg ctgccggagc cgcggtgccg gatgctctgg
     8401 tcgtgtccaa tgtggccgcc ggcatcgtag tgggcaagtt cggaacggcc agcgtgaccg
     8461 gagcggaatt gaagaaggct ctggaggaga aggatatcca gacgccctcc tggcatcacc
     8521 gcgacaatat cctgacgccg gaggccgcgg cggagctggc ggaacgtttc cgacgtgaga
     8581 agaaggtggt tggatttaca aacggctgtt ttgatttgct ccacctgggg catctgcatt
     8641 cctttatgaa ggcgcgtgaa gcgtgcgacg tgctttttgt agggttgaat acggacgcct
     8701 ccatcaagcg gctgaagggc gaggacaggc cgattaataa tgaggagatg cgttctctgc
     8761 tgctggcatc cctggatttt gtcgattatg tagtgccgtt tgatgaagat acggctctgc
     8821 cgctgattga gaagctgcgt ccggacgtga ttgccaaaga ggggtatccc ctggaatgct
     8881 ggccggaagg acagttcgtg aaatcttatg gagggcaggc tcttgaattg ccccgcctgg
     8941 agggcttttc ctccaccaat atcatcaacc gcatgaaaag cagcccggaa tgagcgaata
     9001 catcaggaac cagattttag ggatagcgga caatttcaaa gccctggctt ccatggccgg
     9061 ggacattgag caggttgccc gcatttgtac ggataccctt aaggccggga ataagatcat
     9121 gttctgcggc aacggaggct ccgctgcgga ttcccagcac ctggccgccg aattggtggg
     9181 gcgttacaag ctgaaccgcc ccgccatgaa tgcgctggcc ctgacggtgg atacttccat
     9241 cctgaccgcc gtgggcaatg attacggtta tgagacggtg ttttccaggc agctggaggg
     9301 agtgggccgt cccggagatc tgctggtggg gctttccacg agcggcaaca gccggaacat
     9361 cgttttggcg atggagctgg cgcgccggat gggcgtccgg acggtggcct tgacgggccg
     9421 cggaggcgga gagatgaaag aagttgcgga gttttgcatt gccgttcctt ccgatgccac
     9481 gaacaacatt caggagatgc acattgcagt ggggcatctg gtttgcgaac tggttgaacg
     9541 ggagatatat ggcggggaag gctttgtttc tggacaggga cggcgtggtt aatgtggatg
     9601 gcgggtatgt gcaccgcata gaggacttcc ggctggttcc cggaattctg aatctttgct
     9661 gccaggctaa agagaagggg tatctggtgc tggtggcgac caaccagtcc ggcataggac
     9721 gcggcatgtt ttctgaagat gactttgagc ggttgacgga atatatgaga ggggtgttcc
     9781 gttcgtccgg tgcggagatt gcaggtgtgt ttcactgtcc cagcgcggat gacggacatc
     9841 ccgaccggaa accgaatccc ggcctgtttt tgaaggctgc cgccgcgcat gggctggata
     9901 tgaaagcctg cgtgtccgta ggtgaccggg agcgggacat ccgggcggct cttgccgccg
     9961 gagtaggccg caatttcctg ttttctgcgg aagatgtccc caccctcgcc acggcccgtg
    10021 tggagacgtt ggaggaagtg gcggcaaggc tgtaggccgg gaatagttgc ctggggaaaa
    10081 tcgctgcggt ttgtccctgg agttatcccc tgcatcggtg cgggcgacat ggaaccggtg
    10141 cggggcaaag tattttgaca tacggagagg agcctgttag aagtggaaca atccggccgt
    10201 agtttcgtga acctggcctg tacaggcttt ttatttttac acgtatgaat ctgatacttt
    10261 tccgatccat gatgactgcc gccgcgctgg gggccggcct tgcccttcct gtttctgccc
    10321 agtcttcttc tccggctttt tccgcttctc ctgaaggttc ccgcgccgga aaaacggcca
    10381 agatgccggt gtatcccatt ccgcagaaga tgaccaggag cggcggttcc gtgacggttc
    10441 cggccctgaa gctgttgggc gcgcgggatg aacctaccgt gaatgctctg aaaagcatgt
    10501 tttccctcgc tccaaacggg ctgcctgtgc agatgattat catcaaagga agcaaaaagc
    10561 ctgccgggaa tgttccccgg aaggccgggg cttattccct gaacgttacg cctcagggaa
    10621 tacggatgac ggggtatgac gacgaaggcc tgttttacgc ggcccaaacg ctgctgcagt
    10681 tggcggagaa gacttcttcc ggcgtgacgg tgccggaagt ggaggttctg gattggccgg
    10741 atgttccttt ccgcggtacc attgaagggt tttacggtct gccctggggg caggaaggtc
    10801 gcatcagcca gttcaaattt tacgggaagt ataagatgaa tacttacatc tacggcccga
    10861 aggatgacgt gttccacggt ttttccaagc gctggcgcga gccttatccc gcagatatgg
    10921 cgaaggacct gaaagagctg gtgaaggtcg cgaagcagaa taaggtgaat tttgtctggg
    10981 ccgtgcatcc cggagcggat attcactggg gcgaggcgga ccggaaggcg gccgtgaaga
    11041 agtttgagat gatgtatgac ctgggattcc gttcctttgc cgtatttttt gacgacatcg
    11101 gcggggaggg ggccaaaccg gagggccagg tggagttttt gaattacctg aataaggagt
    11161 ttatccacaa aaaaccggac gtgacgccgt tgatcgtatg ccccacagcg tattccggcg
    11221 gaggtggccg ctaccatgag gtaatggggg aacatctgga caaggatata ggcatcatgt
    11281 ggaccggttc cagcattgtg agcgatatac ggactcctgc gttgaagggg attaataagt
    11341 atttgaagcg tccggcgttt atctggtgga atttcccggt gacggattac gtgcgccatg
    11401 ccctgttttt ggggcgcacc tatggcgtgg atgcggacgc gatgccattt atgcaagggt
    11461 ttgcttccaa tccgatggat aagccggagg cttccaagat ttccctgttc agcgtggcga
    11521 atatgacctg gaacgccaag gcctatgatt ccgacaggac atggaaggac agcattcgta
    11581 ttttgttccc cggctgctcc tccgccatgc agacgtttgc cgatcataat agcgacggag
    11641 gccccagcgg acacaattac cgcaaagagg agtccgtaga gattgccccc gtggtggagc
    11701 aggtgctgga actgtgccgc cgcggggcca gggtgtctgg aagcaaggcg tttgaccgcc
    11761 tgaaggctga gttcgcgaag atggcccaag ctccggccgc gattcgggcc aagtctaata
    11821 atcccgcttt cgttgcagag gtggagccgt ggctcatcca gttcgagtcc ctggggaaag
    11881 ccggggtgaa cagtatgcgc atgattgagg ccacagaggc ggggaatgcc gctggagcgc
    11941 tgaatcatgc catggaggcc gcctgcctgc ttgccgagat gcagcgttac agcagggaaa
    12001 tcagcaaggc catcaataag cacgttacgg aagtgaccaa gaagaattcc ccgtggcaaa
    12061 ccgccgtgaa gccgtccgaa ctggtgatgg ctcccgctgt gcgggaactg ctggacatgg
    12121 gttccacccc cgtgctttcc cgcgtgagcg ggcaggccgt tggccgtgtg aagccttatg
    12181 tttccacaaa gtccaagatt ggcatcgaga agatgctgga tgacgacccc gagtcgttct
    12241 attactgcaa agaggttcag aagaagggtg actttttcgg cgtggatctg ggagttccac
    12301 gggagatacg caccgtttcc attgtgatgg gccgcaatga ttccgatacg gacgctgtga
    12361 acaggggaca gttggaggtt tccctggacg ggcagtcctg gtcttccctg atgccggagt
    12421 ccagcgggct tcgcgtggag taccggggaa atggcaaaaa aagccggttt gtgcgttacc
    12481 gcgctacggc gcagggggtt ccgggaggca agccggacgt gtggacggct atccgggatt
    12541 tcaaggtgaa tgctcccgcc gcgccttccg tactgacgga tgctcccgcg ttcaggaatg
    12601 ccgtggttga agcaggagac agggatattt ccttgaagcg catcatggag gtgcatcccc
    12661 tgccgccgaa aaagttcttg ggccttcaaa ttccggcggg agcgtccgtg gagtccgcct
    12721 ccgtcaattt gaagacgccg gatatgaagt gggccaagtt gttcatttcc atggatggaa
    12781 agtcctgggc ggaggttccg ttgaaggagg acggttccgc ggatattggc ggagtgatca
    12841 agggcatcag gctgttgaac gcctcctcct ccccgcagga ggtgactctg gaagagttcc
    12901 ggctgaatct tgccaacaag ggtgggaagt ccgccgacag cggagctgcc ggggatttca
    12961 acctggcaac gttcctccag gtggagctgt ctccggagag ggcggagatt ccctgtgatt
    13021 ttccccgtgc gggttccgtc attgtcctgt ccgacgggaa ggaggcttcc gtgctggcct
    13081 gtggggctga cggacggtgg gttcccgtgg gcaatctggc caagggcagg aaagttaata
    13141 cgttcagcct gaaatccgtc aaaaaaccgg tgaaggccct cggcctgacc gggaaaaagg
    13201 gaagttccgt aaatattttt gaagtaatct ggaaataacg gatggcatcg ggaagttttc
    13261 cgccgggtgg tgtccgccgg ccctggggcg gctgtttctt ccgtggaatg gccgtcctgg
    13321 attgttagcc cttggtttgc cgggaggctc ttttttccgc caggtgcatg cagccgagga
    13381 cgagggcgat gatgaccaga ggccccagga tgtccgcata ccccaccatg cccgtgttca
    13441 cgggggccac gcaaagcagg aaccaccagg ccagggccag caggcgaagc aggggcgggg
    13501 ttccgctgcc ggagaggaag acatagatga cgagagccca gaattgggga aaacggggca
    13561 gcgtgaccag ccattcccgg tggtcccctc cgggccagac aagcgtaccg tgggggccgg
    13621 ctgcgggaag gtctccccac atggcgcctg agggaagcgc catgttcatc agcaggttca
    13681 ggaacaggcc cgcatatacg gttatcatca aggccctcca ttgggcgcct gttcgcaccg
    13741 ccaggaacag gaaggccggc accagcagga agggagtcag ttccagggct cccatccaga
    13801 gagggagatt tgttcctatt tctgcaaggc ggtccgcgcc ttgtggaatg gcggaaagaa
    13861 cggcccagta caggagcatc agaaagatgc aacggtacct ggcgccccgg cttaccgggc
    13921 gttcttcatc cggaggaagg cccagccatg ctccggatga gggctggtcg ggggaggatt
    13981 gcatggcggg ttgggaatgg tgcttttgta acgggaacgt gcaggtttgt caataaagcc
    14041 cggttcagat ccggaaccgg gattgttttt caacaaaacg ggcattttta ctgtccacat
    14101 tgtgacaaag ttgttgcctt cggcttcttg cctccggcgg cttcttctgc tagtcggtgc
    14161 ctgaaaggcc gcggaaaaac ggctgtgcct cagtatccca ttctttttgc ttatgacccg
    14221 ttccagattc ctgatgattt taccggccct gtttttatgt ctgctggctg tttcctgcgg
    14281 aaagaaggag aaggaagatc ccaatgtcgt agagttgaat ttcggtcatt ttcccaacgt
    14341 gacacatgtg cagggcctgg tggcgcatca tttttcccgg caggggaagg ggtggtttga
    14401 agaacggctg aaggaggcta ccggaaagga tgtcaggatc aactggtacg tgtacaatgc
    14461 gggccccagc gccatggaag ccgtgttcgc ccggtccatt gaactggctt atgtggggcc
    14521 cagcccggcc atcaatgcgt ttgtgcgttc ccgcggggag gatatcagga tgattgccgg
    14581 agctgtggaa ggaggcgccg ccctggtggt tccgaaggat tcctcgctga aggagcctgc
    14641 ggatttccgc ggcaaggtga ttgctactcc ccaactgggg aatacgcagg atgtttccgc
    14701 ccgcgcctgg ttttcccgcg gcggcctgca tgtgacgcag cgcggcgggg acgtgactat
    14761 tctgccgact cccaaccccg aacagctcag cctgttccgg cagggcaagc tggacggcgt
    14821 gtggacggtg gaaccctggg tgagccgcct ggtgctgacg gcgggaggca gggtgctggt
    14881 ggacgagaaa gagtccatcg cgaccgtgct ggtatgtgga gcggagtttc tcagggaaaa
    14941 gccggaggtg gcgaaagccc tggtgcaggc gcatgaggaa ctgaacgaat ggataaggct
    15001 gcatccggat gaggtccagt caatcgtggt cagggagctg gaggatctga cgcattccag
    15061 aatagacccg gcattgattg cggaggcctg gaaaagcatc gtcatcaagg acaggatttc
    15121 cattcccaag ctccggcagt ttgtgcagga cgcccatcag gccggattta tgaaagaagt
    15181 tccggatgta ggcggactgg tggtgccgga ggcggcggag gagcagttga ccatggcgaa
    15241 ggaggcgggc ggaagatgat tgcgggtgca gaacattgcg gccccggagg ctgcaagctc
    15301 cgtattgccg gcgtgagcaa ggtgtttgac gggaggcgcg gaaaggtggt ggctctggag
    15361 ggcattaatt tgaatatcag aggaggcgag ttcgtgtgcc tggtgggggc cagcggctgc
    15421 gggaagactt ccctgctgaa tatcattgca gggctggagt ttccttcttc cggggtggtg
    15481 gagctggacg gggagcctgt gacgggaccc ggacgggacc gcactgtgat gtttcaggag
    15541 tccgccctgt ttccgtggct ggatgtgctg gggaacgtga tgttcggctt gaagctggtg
    15601 cccggactga ctcggggaga ccggcttgcc atggcggaga agaatctgga actggtgggg
    15661 ctgaaagagt gtgcccatgc tcatattcat gaactttccg gcggaatgaa gcagcgcgtg
    15721 gcgcttgccc gcgccctggc tgccagcccc cgcattttgc tgatggacga gccttttgcc
    15781 gccctggacg ccatgacccg cgaacagctt tatcaggata ttcaggagat tcatttacgc
    15841 tgcggcatga cgattgtttt cgttacgcat aatatgcgcg aggccgtgtg cctgggggat
    15901 cgtgtggtgt tgctcactcc acatccgggg cgcgtctgtg aggaatattt cgtggatttt
    15961 cctcatccta gggatattaa cagcagtgat ttggcggaat tgtccgcccg cattacccgt
    16021 gatttgaaag gagccacggc atgaacagga acaaatggtc caagtggggg gcgcacgcct
    16081 gttcgctcgt gtttttcatt ctcgtggtct gggcatggca gcatttgagc gacctgaggg
    16141 tctggaagcc ttatttgttt ccctctccgc tggaagtatg ggagtatctg cgctcttcat
    16201 ttcaggacgg gtctctggag gaggcttcat ggattaccat gaaaaggctg gtgatggggt
    16261 atggaatcgg cctggtgacg gggattcccc tgggcatgct gtgctcccgt ttccagctta
    16321 tgcagaatac tctgggactg gtttccctgg ggttccaggc tctgcccagc gtgtgctggg
    16381 ttccgttggc aaccctgtgg ttcggccaga cggaaggggc tatgctgttc gtggtgatta
    16441 tggggacgct gtggtccgta atcctggcta cggccaacgg catgcggaac gtgccgccca
    16501 tttatgcccg tgcggcgcgc accatgggag caggacccat ttattgcctg attcatgtga
    16561 cgcttcccgc ttccgctccg tttgtggtga gcggaannnn nnnnnngggc ttttgcctgg
    16621 cgctccctga tggctgcgga aatttttgtt cccatcctga ccgggttcgg attgggccag
    16681 ctgctgcatt tcggccgcga actgaacgcg atgaaccagg tcgtaggcat catgttcgtg
    16741 attgtggtga ttgggctgct gtccgacaag attctgtttt ctcctctgga acggtttctg
    16801 caccgccgct gggggacagg acaggcctga agcggcggga acagaaacac ggagaggggc
    16861 tgcagagcgt tccggcgata tggtgcccgt tatttgccgg aagaatccag ctcctttttg
    16921 agcgcgcgga cttccttaat cagtgccgga agttttttga gggcggcata ttggcgggta
    16981 gcgtccttgt aggggaaagc cggcatgccc cagtaagtag agttttccgg aatgtccgag
    17041 atgactcccg ttttagcgcc cagcgtggat ttggaaccga tgttgaggtg tcctgagatg
    17101 ccgacctggg cggcgatggt ggcataatcc cccactttgg tgctgccggc tatgccggat
    17161 tgggcgacga tgatgcaatg cctgccgaca acgacgttat ggccgagctg gatcaggttg
    17221 tctattttgg tgccttcccc cacaatggtg cgcccgaatc tggcgcggtc aatagttgtg
    17281 ttggccccta cgtccacgtc atcccccagt tccacaattc ctacctggtc aatgcccacg
    17341 tagcgtccat tgtctcccat caggaagccg aatccgtcgg aaccgatgac ggctccgggc
    17401 tggatggtca ccctgttgcc gagtttgcag cgttccctga tggtaacatg ggcatgcagg
    17461 cggcagtttt cccccatggt gacgccgtct ccgatatcgc agccgttgcc aatgtccgtt
    17521 ccgtctccga ttacgcagtg agccccgatg caggtatagg ctcctacatg gattttgtcc
    17581 gggttgaagc tggccgtggg gtcgatgatg gctgtgggat gaatgccggg ggtgaaccgg
    17641 taggcggaag ccatgaaata tttcaccagg acgttgaatg ccatggaggg gttgtccact
    17701 tccacgaagg cggcgtgttc cggatacgca ggcaggcccg gcggcaccag cacgattccg
    17761 gcagaggtat gcaggaagtc ctggaagtat ttttcattgc caaggaagga ggcttcttca
    17821 cgggatgcgt caagaaggga agcgaccccg aatacggtca gttcggggtc gccggaaagg
    17881 atttttcccc cggtgagggc ggccacggct tcaagtgtaa gcttcatggg tatatgattc
    17941 cgccttcccg ggggtaatgt caagggttag ttggccgggg ccgcgggagc ggcagggacg
    18001 gccggagcct ttttcttgga ggggtcaaac ccttcggggg cgtccttgtt gagttctttc
    18061 atcactgtgg gggtaatgtc catgccgggc ttcgtgtaga cgaatacctg gttggaccga
    18121 agggcctggg cgcttttgtc cagaacgagg tcatagttgc ccttggtggc gatgccttcc
    18181 gtgatttttg tgatttctcc catgattttg gcggaacgca ctttcatttg ttcctgaagg
    18241 gatttaagct ggcgttccac aaatccgcgg cgttcctgtt ccagcgcaat gacttcctga
    18301 cgcttgatct gggctttttg ctccagctcc tttttgtcct tttcattcag catgggatcc
    18361 tgaagccttt tgaccatgtc gttgaagtcc gcttccatct ttttgatggt ttccgcgcgg
    18421 gtgttgttgg tttcctgcac cgtctgggca gccttgtcta cttccgcctg ggcttcatgg
    18481 gtcttgtagt agtcggcaaa gagtttctgc acatccaccg tcgctacctt cagttcagcg
    18541 ttggccgtgg cgcttaatgc cattgctgct gatacaccta atgctgtttt gaagaatctc
    18601 ataattttta gcgtcttctg actttaccag tttacatgtg gaatggcatg cgtcaacctt
    18661 ctagcaatat ttcaggattg aacttttgtc atcatggggc cactttaggc gcatgcaaac
    18721 attttccacc aaggcccagt tgagggct
//
LOCUS       DAOMSU010000010        18554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_89_length_18554_cov_3.109249, whole genome shotgun sequence.
ACCESSION   DAOMSU010000010 DAOMSU010000000
VERSION     DAOMSU010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18554
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89_length_18554_cov_3.109249"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="PK077_01000"
     CDS             complement(<1..394)
                     /locus_tag="PK077_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRN22910.1"
                     /translation="MKEAASSQSPYSGNHSVKQAVDGSMESANWHVPGVHHVEGEFVF
                     EVPETIHYIIFSGADFNEIAVSAMSGSSWKDLGKFDIGGSRMIRFKKPLQKVRKIRLT
                     VDYPEGSSPSFTVREISFYKRVESVLNRK"
     gene            657..1172
                     /locus_tag="PK077_01005"
     CDS             657..1172
                     /locus_tag="PK077_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22911.1"
                     /translation="MEYYAMLLSMNEGRPCHQSVNKRVKWLGALAVLLFLLGTGLFSF
                     LWNVPLNTLRPAHAIAAPSAILVRENSSPERCRMALEPSFLASIQPFLKHYVETVTNA
                     GRLDFSSHVPQEVEIRMEGLSIGLSPSLAVFNFRTKYGFSMQTSRKPDASDRAIYQWL
                     KNRMKAEKREK"
     gene            1313..2440
                     /gene="msrB"
                     /locus_tag="PK077_01010"
     CDS             1313..2440
                     /gene="msrB"
                     /locus_tag="PK077_01010"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HRN22912.1"
                     /translation="MKRIYHGPWTLAGLACAILAAVAMLSFFSCGEKGQASSNDKPHM
                     DNKDLKTIYLAGGCFWGVEKYFSLMPGVKETEAGYANGSTASPTYEEVCSGKTGHAET
                     VKVVFDPEELSLPFLLERYYSIIDPVSVNKQGNDRGIQYRTGIYYADEKDKPVIETSL
                     KRLQQHFRQPLAIEVQPLRQFSRAEKYHQRYLDKNPGGYCHIPPAKFREASQTRENEP
                     VYRKKSDEELRRVLTREQFAVTRNNATEPPFRNEYFNNDRPGIYVDVTTGEPLFLSTD
                     KFDSGCGWPSFSRPIKEELIQEKQDFSHGMRRTEVRSKTGDAHLGHVFTDGPKEQGGL
                     RYCINSASLKFIPEQDMEAQGYGKYLPLLHREGTDRKAAEK"
     gene            complement(2813..3289)
                     /locus_tag="PK077_01015"
     CDS             complement(2813..3289)
                     /locus_tag="PK077_01015"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRN22913.1"
                     /translation="MVPLTVPATVPMTSYGPHARVVVAGIDYTEVHDEWMNPDIMKKA
                     TPGNTRVVISRGRQRGQLMVGDEVAMDFPVCVGKASHRTPVGHFRITEKAVHHVSNLY
                     DASMPYFMRLTDSGIGMHVGPVFQTPQSHGCIRMTRSSCVPLFKTVKVGTPVTIVQ"
     gene            3646..6399
                     /gene="acnA"
                     /locus_tag="PK077_01020"
     CDS             3646..6399
                     /gene="acnA"
                     /locus_tag="PK077_01020"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HRN22914.1"
                     /translation="MTKIAKSTFTTGTGTPGQFYSLPALAENGYPRLNRLPVSIRIVL
                     ESLLRNCDGLKVTEKDVDNLASWNANNPGSYEIPFTVARIVLQDLTGVPLLVDLAAMR
                     SAVAGLGKDASVIEPLVPVDLVVDHSVQVDWAGCTDALEKNLDIEFQRNAERYEFLKW
                     GQQAFQTFSVVPPSVGIVHQVNLEYLAQGVMEKDGVYFPDTLVGTDSHTTMINGIGVV
                     GWGVGGIEAEAGMLGQPVTFLVPEVVGVHMTGELREGVTATDLALHVTQMLRSHGVVG
                     KFVEFFGDGAAALPLADRATVANMAPEYGATMGFFPMDERCSDYLRQTGRSEEAITTY
                     ENYFKAQNLWGMPRNGDLDYTDQLELDLSAVEPAVSGPRRPQDHIRLNSIRDSFRKLV
                     SMPVAEGGYGKKESPEVTVSMHSPAGVPVPVDGFSQEAKLKDGSILIAAITSCTNTSN
                     PGLMLAAGLLAKKAVERGLNVPPHVKTSIAPGSRIASDYFATNSLQESLDALGFETVG
                     YGCTTCIGNSGPLNPELEQAVRDNDIVAASVLSGNRNFEARIHASIKTNFLMSPPLVV
                     AFALAGRVDIDFQTEPLGTDRSGNPVFLKDIWPSSGEIAEAVAKSRNPEAYRELYTIT
                     PDRNPRWAAVYAPEGSVFQWNEQSTYIQDPPFFSGFNGQPRTLADITDARPLGIFGDS
                     VTTDHISPAGAIRETGPAGLYLQGMGVDRKDFNSFGSRRGNDRVMTRGTFANVRIKNL
                     MVNGTEGGYTLYFGNRAVPAPDKEIIPAAGTPAFIYDAAMAYAQDGVPLIVIGGEDYG
                     MGSSRDWAAKGTNLLGVKAVITKSFERIHRSNLIGMGVLPLNFADKADYDRITPLKDA
                     TFSILGLNNGIAPRQQVTLRVQPADAEAFDVPVIVRIDTPIEKEYYLAGGILQYVLTQ
                     ILR"
     gene            6589..7254
                     /locus_tag="PK077_01025"
     CDS             6589..7254
                     /locus_tag="PK077_01025"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRN22915.1"
                     /translation="MEYRFVWTGFFTVFAALTFCSCSQNDKMRPPAVSFKIQKAPVVP
                     RTPGQMGREVGIKVSYMPKNTYARKRASSMRPRFITIHSTANPKGDANAHSRYLNSGK
                     SRSLNWHFTVDQFGAYQHIPTTETGHHADHSGPGDQYSVAIEMCECTTHNPVVIYNKT
                     AKLAALLMMRYNVPLRNVVPHNYWSGKNCPAPLMTNGRPGYKWSWFISRVDYYYRCLQ
                     AGR"
     gene            8208..8612
                     /locus_tag="PK077_01030"
     CDS             8208..8612
                     /locus_tag="PK077_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22916.1"
                     /translation="MKKILQLLGILTFVICNYGNAKELKCEYELKNLNIEPVRVANAK
                     RTYTVYISCKYTSVHHTGETGGPIPLRKTITPVVTGTDDEKEGKAEAYAEGESTVIAM
                     ARLRLTEECGLQCGNCNEVGCTCVGCNDNINK"
     gene            8636..9067
                     /locus_tag="PK077_01035"
     CDS             8636..9067
                     /locus_tag="PK077_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22917.1"
                     /translation="MKPLLKIAFLSIACFPCFCINNAAGSEDVIPGSQKIDRENSIIN
                     PIILTGIKKTDDIRESQKEYIRKHYEGYGILGYIYTMHDGKYIQIISIRNDQGQEKLI
                     YFDMTDIYRKLGKSRDKKTRKDIKRLIEDHKPLEKEPAESV"
     gene            complement(9289..10461)
                     /locus_tag="PK077_01040"
     CDS             complement(9289..10461)
                     /locus_tag="PK077_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22918.1"
                     /translation="MKCLLFAVLAWCGAVCAPAEEYAFPGEREWISKFGDVVKGSFHS
                     CTGAGAVLKIGRRHVEIPLTRLDEEDAALAASLTGAEARRNLKLAAMLWPFLMKELQK
                     VTWTPAEKNISPRYHEMARKKNAGALTGTRKVDGLVCATSLSIIQGPPFRIREYFRPQ
                     ARPESGRGQKNFDVVLEQGSRCAYYDMDKGVLIPSKCLSEERTEMRAPVSSLLPPAEQ
                     LAARESKNYRNADYSLLVENRPVKGAYETYKPNSLGRKKLVMYWNTEGEMVCGVMKLD
                     YDNMRVAALFDGRRKLMNAPGLGYAVILSRLDKKGRGLKEKGRYYHVALAADGLPVYY
                     HEGPEDEAAMNPLLYGKLRDSEKKAALAEEKRREQAFRARREARRRLVESARKRNT"
     gene            complement(10574..>13152)
                     /locus_tag="PK077_01045"
     CDS             complement(10574..>13152)
                     /locus_tag="PK077_01045"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HRN22919.1"
                     /translation="SVATDLARVLGKELGKFTRDKSGAFYQNDRAIITSAVGHLLEQK
                     KPMTEEGKSLPWKFDYLPVIPRKFELEPIKQSEDRLKKVLQLARSKDVTEIVNACDAG
                     REGELIFHNLVRYGKWTKPTRRLWMQSMTDEAILNAWHGMRSEADMKPLTNAAVCRSE
                     SDWLVGLNSTRALTILQSRGGFNVTPAGRVQTPTLAILTQRELEIQAFEPEPYSEVWA
                     EFDVQSGSYSGRWIDRDWKKNDHPHSRAERIWDPEQAAVIRDRCRGKAGTVTEEKKPV
                     TTVAPLLYDLTSLQREAANRYGFSAKRTLQLAQECYEKHKVLTYPRTDSRYLPEDYLG
                     KVYGIVGTVAEQNPEFAPFARDILDNKRIKPNRRVFDTKKVSDHFAIIPTGKMEKLTG
                     DAQKLLEMVMARFLAVFFPAAVFEDTRRTTLITHQDGVTDAFLTTGRVLVEPGWQAVY
                     GRKAGTSSKEELVPVRDGEQAALREIEIRNAMTKPPARYNEATLLAAMEGAGKLVHDE
                     ELAAAMSERGLGTPATRASIIEGLISQEYIVRDGRELLATRRGIELIALLERIGLKTL
                     TSPSLTGEWEYKLKQMEQAALPRDSFMEGIKTLTGEIVKRVKDFREAQQQVELPEMEL
                     DCPSCHAHGLKNTLDAVSCRSCKFSIRKVISGRDMTDEELKTLIVDGRTGILHGFTSR
                     FGKPFEAGLELNDKFRATLYFLAREEDEAAGVEEVVKVASVAIPDMGEHDVMETAKAW
                     KIPSLTLGKEHAAVSVSRTILGREIPLDQVLKILAEGKSDLMKGFISQRTKRPFDAYL
                     VFNAKTGKIGFEFPARERKYPAKNAAGKTEGKAEKTGRTAAKTAGKDSKSGGAAKKGR
                     KQ"
     assembly_gap    13153..13162
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            13422..13859
                     /locus_tag="PK077_01050"
     CDS             13422..13859
                     /locus_tag="PK077_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22920.1"
                     /translation="MMKTGFSFVLLPSCLMLLAACSPTPSDRIAKNMPAYEQLPKEHQ
                     AKVARGELEKGMSPMAVLLAWGEPEQKMAGRMNGKNTERWIYTRSGTGWSLGLGGGGF
                     RGRDSGIGTGIGVTMPLGSRPPVSCNVLFEDGKVIGWEAVNGF"
     gene            complement(13928..>14590)
                     /locus_tag="PK077_01055"
     CDS             complement(13928..>14590)
                     /locus_tag="PK077_01055"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HRN22921.1"
                     /translation="RIAPPSNRREFAESVVHAHTYDWLVFSSPNGVERFFQAFFAVYR
                     DIRSIGGARIAAIGPGTEAKLREYGLAVDIMPKKFVAEGLVKAFRDAREEIGTIEHST
                     FLWVRGEEARRVIYDGLNALGAIVDECIAYKTEAETEDVAGAQAAFRERGADIVTFTS
                     SSTAENFFKLGLPWPEGCRAASIGPVTTATLKELGHTPSITAKTHDINGLVEAIVKAA
                     GK"
     assembly_gap    14591..14600
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14601..15512)
                     /gene="cobA"
                     /locus_tag="PK077_01060"
     CDS             complement(<14601..15512)
                     /gene="cobA"
                     /locus_tag="PK077_01060"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HRN22922.1"
                     /translation="MAGFLVAKKRMVELNGGMQQGIVYLLGAGPGDPGLITVRGRELL
                     GMAEVLVYDALSSAEMLNWVPVACERIFVGKRASRHALPQEEINALLVRLGRAGKKVV
                     RLKGGDPYVFGRGGEEADALHEAGIPFEVVPGVTSAIAGPAYAGIPVTHRGYCTQFTV
                     FTGHEGENKKASSLNLKGIAEAQGTKVMLMGMQKLADVCEALIGYGQEPSVPAAAVQW
                     ATTGIQRTVTGTVKTLPARVQKAGVGAPAVVVIGDVVKERESLNWFEKLPLFGKRIVV
                     TRTRAQAGELSARLRRLGAEVLEMPVIR"
     gene            15675..16172
                     /locus_tag="PK077_01065"
     CDS             15675..16172
                     /locus_tag="PK077_01065"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRN22923.1"
                     /translation="MDPLHSPTVTLVEPTKKEKSTLFKPSLYGGRMTRKQYWVRYFTF
                     IMIGRIICNSFIETAFDLTEFIVSVILLLLFEIFYTLPITIKRYHDAGYSGSLAVWLA
                     VISTIMLFLAIFTSPSSAVGVCSLLMCIILGIVNLVILCKDSEIDDNKYGPSTKYPDL
                     VKKEQ"
     gene            16511..17269
                     /locus_tag="PK077_01070"
     CDS             16511..17269
                     /locus_tag="PK077_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HRN22924.1"
                     /translation="MIELFNIDLLSYTVQTAETAPTHSYTIYWLILLGSMLLSWLVSA
                     RMKSRFSEYSQISIPVTGRQVAEQMLKDNHITDVKVISTPGHLTDHYNPADKTVNLSE
                     SVYNSNSIAAAAVAAHEVGHAVQHAQAYHWLGLRSALVPIVQLSSNLVGIVLMIGIVL
                     LAMGGSPWVLGLGIGLFAVTTAFAFITLPVEFDASARALKWLDRSHLMNYFDHGRAKS
                     ALFWAAMTYVVGALSSLAMLLYYVFIFLNARGRE"
     gene            complement(17746..18189)
                     /locus_tag="PK077_01075"
     CDS             complement(17746..18189)
                     /locus_tag="PK077_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22925.1"
                     /translation="MNIRKKVIFAVMALAIPSSWADIPESSVPVAAVSAVKGAWPRAG
                     FIEWDASGSGKLYEAEFKINGFEFDVKVTPEGKIVRVKEEIAVSSLPEPVRAAALKPY
                     PGGRIREADKVTQGELIRYKVEVMDNLDDYDILLTPDGTILYIDQ"
     gene            complement(18238..>18554)
                     /locus_tag="PK077_01080"
     CDS             complement(18238..>18554)
                     /locus_tag="PK077_01080"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HRN22926.1"
                     /translation="RLSIPVGLEIGRTDEWKGRPWAQALRVSYVGDVLQDVPEGTVYS
                     PYSDMSWRGRAVSPERHGFRAEYNTSLQCNERWSVYGGYSLEARGNSLYHRVNAGVAR
                     SF"
BASE COUNT         4261 a         5410 c         4706 g         4157 t
ORIGIN      
        1 atttccggtt aagtacggac tccacccttt tgtaaaagct gatttcccgt acggtaaaag
       61 acggagagga accttcggga taatcaaccg tcaacctgat tttccgaacc ttttgaagag
      121 gctttttaaa tctgatcatc ctggagccgc cgatatcgaa ttttccaagg tccttccaag
      181 acgagccgga catggcggac acggcaattt cattgaaatc ggctccggaa aaaatgatgt
      241 aatgaatggt ctccggcact tcaaacacaa attccccctc cacatgatgc actccgggaa
      301 catgccagtt ggcggattcc atagagccgt ccacagcctg ctttacggaa tgattgccgg
      361 aataggggga ctgggaggaa gcagcttctt tcacgtggag caaaatgtct tccggaacat
      421 ccagcgaagg atattttgcc gcgagggatt tatttacgga gattgtcaga aaaaggaaaa
      481 ataaaaaaat ggaaagaaaa atggaatctt ttctcataca ctacattaat atttaatgat
      541 actatacatg atttccagcg ttttttttca tgcttttcat gttatagtac aaaaattccc
      601 tcatgcgggg acgggaattc ccatctgctt cctggcaaat tctttcatgg atcaccatgg
      661 aatattatgc catgcttctc tccatgaacg aaggccggcc ctgtcatcaa tcagtaaaca
      721 aacgggtcaa atggcttggc gctctggccg tccttctttt tctcctgggc acggggctgt
      781 tttccttttt atggaatgtt ccgctgaata cgttaaggcc cgcccacgcc atcgcagctc
      841 cttccgccat tcttgtcagg gaaaattctt ccccggaacg ctgccgcatg gctctggaac
      901 cctccttcct ggccagcatc cagcctttcc tgaaacacta tgtggaaacc gtcaccaatg
      961 ccggacgcct cgacttctcc agccatgtcc cgcaagaggt ggaaatacgg atggaggggc
     1021 tttccatcgg cctgagccct tctctggccg ttttcaactt ccgaaccaaa tacggattca
     1081 gcatgcagac aagcagaaaa ccggacgcct ccgaccgggc tatttaccag tggctgaaaa
     1141 acaggatgaa ggcggaaaaa cgggagaaat aaggaaatat ggacacaggt atcgaacgga
     1201 atgaacccgc tgttgtctaa tcatttacct ttccggaaaa gccgggggac agcagttgcc
     1261 cggcccaggg agccgctgct cccgcaccgg aaggcgactc catacagcgt ttatgaagcg
     1321 tatttaccac ggaccatgga cactggccgg actggcatgc gccattttgg ccgcagtcgc
     1381 catgctctcc tttttttcct gcggggaaaa aggccaggca agcagtaatg acaaacctca
     1441 tatggacaac aaggatttaa aaacgattta cctggcggga ggctgtttct ggggagtgga
     1501 aaaatacttc tccctgatgc ccggcgttaa ggaaacggaa gcgggatatg ccaacggctc
     1561 cacggcttct cccacgtatg aagaagtatg cagcgggaaa acgggccatg cggaaacggt
     1621 aaaagtagta tttgacccgg aagaactcag tctgcccttt cttctggaac ggtattactc
     1681 cattattgat cctgtttccg tcaacaaaca gggaaacgac aggggaattc aataccgcac
     1741 gggcatctat tacgcggatg agaaggacaa acctgttata gaaacgtccc tgaaaaggct
     1801 ccagcagcac ttcaggcagc ctctggcaat tgaagttcag cccctccgcc aattttcacg
     1861 ggcggaaaaa tatcatcagc gctatctgga taaaaaccct ggcggatact gccatattcc
     1921 acccgccaaa ttccgggaag cgtcccagac ccgcgaaaac gaacctgttt accggaaaaa
     1981 atcggacgaa gagctgcgcc gcgtcctgac acgcgagcaa tttgccgtca cgcggaataa
     2041 tgcgacggaa cctcccttcc gcaatgaata tttcaataac gacagacctg gcatttatgt
     2101 ggacgtcacg acaggcgaac ctttattcct gtccacagac aagtttgatt caggctgcgg
     2161 ctggcccagc ttctcccgcc ccatcaaaga agaactgatt caggaaaaac aggatttctc
     2221 ccacggcatg aggcgcacgg aagtccgcag caaaacggga gacgcccacc tgggccacgt
     2281 tttcacagac ggcccgaagg aacagggcgg actgcgctac tgcatcaaca gcgcctccct
     2341 gaaattcatt cctgaacagg acatggaagc acagggttat ggaaaatacc ttcccctgct
     2401 gcaccgggaa ggaacagaca ggaaagctgc ggaaaaataa tccggagaaa aagcagggga
     2461 ggccaatcct tcttctccat taatctttta caacacggag gaacaaatgc ggccccggga
     2521 gaacaagcac catgtcctct tcacgaaata cgggcagccc gttcattccc caagttctgc
     2581 ggtcaagcag acggcttccc tccatgccgc taccgatgaa tgaatactat acagtcggca
     2641 gcggcatgga gggttcgggt tgctgaggca atgcgacttg cggcaagtac cgtttatcta
     2701 catgccgttt attaaaatag cggcggatga cgtttttttg tttaaaaaaa tgagttttta
     2761 aaaaacgcgg ggaatttttc ttccggaaaa gcgcccggaa attttccggg cattactgta
     2821 caatcgtaac gggcgtgcct accttaacgg ttttgaaaag cggcacgcag gaagaacggg
     2881 tcatgcgaat gcacccatgg gactgcggcg tctggaaaac agggcctacg tgcatcccaa
     2941 tgccgctgtc cgtcagccgc atgaagtaag gcatggaggc atcatacaaa ttggaaacat
     3001 gatgcactgc cttttccgta atgcggaaat ggcccacggg cgtgcggtgg gaagccttac
     3061 ccacgcaaac gggaaaatcc atggctacct catcccccac catcaactgg cccctttggc
     3121 gtccgcggct gatcaccacg cgcgtattgc ccggggtggc ctttttcatg atatcgggat
     3181 tcatccattc gtcgtgcacc tccgtataat caattcctgc tacaaccacc ctcgcgtgcg
     3241 gtccgtagga ggtcatgggt acggttgcag gcacggtcag gggaaccatg cgcaaattct
     3301 cttcctgcgg gccagaacag gcagccagcg ccaacgctcc cgctatgatt gataagatgt
     3361 tttttgcgtt catcatcttg aaacctttca cagggttgga caatactgcg gactcatttt
     3421 caatgttcca tgacgcactc catgattcct gcggcgtgca tggagaaaaa atgccggaaa
     3481 aagcggcagc cccggttcag agaaaaattt ccccggcttc ggaagggggg gaaaaatccg
     3541 ccctccgtca cacagagtcc ttgccttatc acatttggcc tgtatagtac ccgcgactgc
     3601 caggagggca gccgcctgtt tacagttaac cctctattcc gcatcatgac caagattgcc
     3661 aagagtacgt tcaccacggg aaccggaacc ccgggacagt tttattcact gccagccctg
     3721 gcggaaaacg gctatccccg cctgaaccgc cttcccgttt ccatccggat cgttctggaa
     3781 tccctgctcc gcaattgcga cggcctgaaa gtgactgaaa aggacgtgga caacctggcc
     3841 tcctggaatg ccaacaatcc cggttcctat gaaatcccgt ttacggtagc ccgcattgtc
     3901 ctgcaggacc tgacgggcgt ccctcttctg gtggacctgg cggccatgcg ctccgccgta
     3961 gcaggattgg gaaaagacgc ctccgtcatt gaacctctgg tccccgtgga cctggtggtg
     4021 gaccactccg tccaggtgga ctgggcagga tgcacggatg ctctggaaaa gaacctggac
     4081 attgaattcc agcgcaatgc ggaacgctac gaattcctca aatggggcca gcaggccttt
     4141 cagacttttt ccgtggttcc tccgtccgtg ggcattgtcc accaggtcaa tctggaatac
     4201 ctggcgcagg gcgtgatgga aaaggatggc gtttacttcc cggataccct ggtgggaacg
     4261 gattcccata ccacgatgat taacggcata ggcgttgtgg gctggggtgt tggcggcatt
     4321 gaggcggaag ccgggatgct cggccagccc gtcacgttcc tggtgccgga agtagtgggc
     4381 gtccatatga cgggagaact ccgcgaagga gtgaccgcca cggacctggc gctgcatgtc
     4441 acccagatgc tgcgcagcca cggcgtcgta ggcaaatttg tggaattctt cggagacggg
     4501 gcggcggccc tgcctctggc agaccgggct accgtagcaa acatggcccc ggaatacggc
     4561 gccaccatgg gcttcttccc catggatgaa cgctgctccg actatctgcg ccagacaggc
     4621 aggagcgaag aagccatcac tacgtacgaa aactacttca aggcacagaa cctgtgggga
     4681 atgccccgta acggagattt agactacacg gaccagctgg aactggacct gtctgccgtg
     4741 gaaccagccg tctccggccc cagacgcccg caggaccaca ttcgcctgaa ctccatcagg
     4801 gacagcttcc gcaagcttgt cagcatgccc gtggcggaag gaggttacgg caaaaaggaa
     4861 tctccggagg tcacggtttc catgcactcc ccggcgggcg tccccgttcc ggtggacggc
     4921 ttcagccagg aagcgaagct gaaagacggc agcatcctca tcgccgccat cacctcctgc
     4981 accaacacat ccaacccggg actgatgctt gccgccgggc tgctcgccaa aaaagcggtt
     5041 gaacgggggc tgaacgtgcc tccgcacgtg aaaacgtcca ttgctccggg ttcccgtatc
     5101 gcctccgatt actttgcaac caacagcctc caggaatccc tggacgctct gggctttgaa
     5161 accgtcggct acggctgcac cacctgcatc ggcaactccg ggccgttaaa tccggaactg
     5221 gaacaggccg tccgggacaa tgacattgta gcggcttccg tcctgtccgg caaccgcaac
     5281 tttgaagccc gcatccatgc ctccatcaag accaacttcc tgatgtcccc tcccctggtc
     5341 gtcgcctttg cgctggccgg ccgcgtggac atcgacttcc agacggaacc gttgggcacg
     5401 gaccgcagcg ggaaccccgt atttctgaag gacatctggc ccagctccgg ggaaattgcg
     5461 gaagccgttg ccaaatcccg caacccggaa gcataccggg agctttacac catcacgccg
     5521 gacaggaatc cccgctgggc ggccgtatac gcgccggaag gctctgtctt ccagtggaat
     5581 gaacaatcca cctacattca ggaccctcct ttcttcagcg gcttcaacgg ccagccccgc
     5641 acactggcgg atatcacgga tgcccgcccc ctgggcattt tcggcgattc cgtaaccacg
     5701 gaccacatct cccctgccgg agccatccgg gaaacagggc ccgcagggct gtacctgcag
     5761 ggcatgggag tggacagaaa agatttcaac tccttcggct cccgccgcgg aaatgaccgc
     5821 gtgatgaccc gcggcacctt cgccaatgtc cgcattaaaa acctgatggt aaacggcacg
     5881 gaaggcggtt acacgctgta tttcggcaac cgcgccgttc ccgccccgga caaggaaata
     5941 atccctgccg cgggaacgcc cgccttcatc tatgatgcgg ccatggccta cgctcaggac
     6001 ggcgttccgc tcatcgtcat cggcggagaa gactacggca tgggttcttc ccgcgactgg
     6061 gcggccaagg gcacgaattt gctgggcgtc aaagccgtca tcaccaagag ttttgagcgt
     6121 atccaccgct ccaaccttat cggcatgggc gtcctgcccc tcaacttcgc cgacaaggcg
     6181 gattatgacc gcatcactcc gttgaaggac gccaccttct ccatcctggg cctgaacaac
     6241 ggcatcgctc cgcgccagca ggtcaccctg cgcgtacagc ccgcggatgc ggaagcattt
     6301 gacgtgcctg tcatcgtccg cattgatacg cctattgaaa aagaatacta tctggcgggc
     6361 ggcatcctgc aatatgtcct tacccaaatc ctcagataac gggatatgtg ccgggcgttc
     6421 ccggtacggc ttcccctcgc ggacgggccc cttcctcccg aaggcgcggc ccgtccgcct
     6481 catccgcacc ggatatacct gcaacttcac ggcacggcct gattagttca tccgaactaa
     6541 ttccaaattc ttcttgcaat ccttccaggc tgttcctaga acaaacgcat ggaatataga
     6601 tttgtctgga cgggcttttt taccgtattc gccgccctga cgttctgcag ctgttcgcag
     6661 aacgacaaaa tgaggccgcc ggccgtctcc tttaaaattc aaaaggcgcc ggtagttccc
     6721 cgcacacccg gccagatggg ccgtgaagta ggcattaaag tttcctacat gcccaaaaac
     6781 acgtatgccc gcaagcgcgc atcctccatg cgcccccgct tcatcaccat ccacagcacc
     6841 gccaacccca aaggagacgc caatgcccac tcccgttacc tgaacagcgg caaatcccgc
     6901 agtctgaact ggcactttac tgtggaccaa ttcggcgcct accagcacat ccccactacg
     6961 gaaaccggcc accatgcgga ccattccgga ccgggagacc aatactccgt agccattgaa
     7021 atgtgtgaat gcaccacgca taaccctgtc gtcatctaca acaagacggc caagctggcg
     7081 gcgctgctga tgatgcgcta caacgtcccc ctgcgcaacg tagtgcctca caactactgg
     7141 tccggcaaga attgccccgc cccgctgatg actaatgggc gccccgggta caaatggagc
     7201 tggttcattt cccgcgtgga ttattactac cgctgcctcc aggcaggaag ataatactgc
     7261 cgcatatgcc gcacgcccct ttgccgcttc cggcataaag aaggcaggaa atgtttcagg
     7321 aaagaacagg aatcacttct ttccctgaaa atatgcattg gctgaacacc gggaattcag
     7381 cgcatctccg gctcgcggaa agcaggatcc gcctccattg atacggctaa tgtacacggc
     7441 cgtaaaagac ggcaaataac acccgtccgg gaacaatctt catcaacatc aaaatccgga
     7501 acacggaccg ggaataagaa atagtgctga aagcttttga tgcctcctga accaccccgg
     7561 aacaggttat ttgacctgaa tccgattcac cggtcccatc ctctccggat ggatcgaaaa
     7621 agactgcggg agactcaaca tcaacaggga aacttgaaaa ccatgaagcg ctttcacagg
     7681 atgctcagat gcggaccatc gaagccagca gcttcttacc cgccatatgg aagcattgag
     7741 ggaacaatcc ggcaccatgt tcatggaaaa ccagcaggaa ctcaaactga aaggctccag
     7801 agaaaacagc ctcctcatgc cagaaacatg gcattttagt ggaatcccgg cccctcggca
     7861 taccagaatc tcccatttta tcccgtatgg cggataaata taaagggccc gcagctcaac
     7921 tgtgcctttg ctgaacgccg cagcatggcg gcatgccccc ggtaaaatcc acgccttcgg
     7981 gcggaattaa agccaatccg ggcattttca acttctcccg agatgcgaag gcgttgtttc
     8041 tccgttggaa cgccggacac tcctattcag cgcctcatcc tccccggggt ctttcccgga
     8101 taatcattat tttgttagat atttttgtaa ctatctatca agcaatcatt atataaaaca
     8161 tattaaaaat atgtttacat atatcaaaat tgtcccataa ttttaggatg aaaaagatat
     8221 tgcagttatt gggcattttg acctttgtta tttgcaatta tgggaatgca aaggaattaa
     8281 aatgtgaata cgaattaaaa aatttaaaca tagagccagt cagggtagcc aacgccaaac
     8341 gcacttacac agtctatata tcctgtaagt atacaagcgt tcatcacacg ggtgagacag
     8401 gggggccaat accgctgcga aagacaatta ccccggtagt aaccggtaca gatgatgaaa
     8461 aagagggcaa agccgaggca tatgcagaag gagaaagcac agtcatcgca atggcccggc
     8521 tgcgtttgac agaagaatgc ggtttgcaat gcggcaactg caacgaagta ggctgcacct
     8581 gcgtcggctg caatgacaac ataaataaat aaaactgatt aataaataaa ctgatatgaa
     8641 accattactg aaaattgcct ttttgtcgat agcctgcttt ccctgttttt gcattaataa
     8701 tgccgccggt tcggaggatg taattcccgg cagtcagaaa attgacaggg aaaattcaat
     8761 catcaatcct attattctta caggaatcaa gaaaacagac gatatcaggg aatctcagaa
     8821 ggaatatatc aggaaacact atgagggata tggcatacta gggtacatat ataccatgca
     8881 tgatggcaaa tatattcaga ttatttctat tagaaatgat cagggtcagg aaaaattaat
     8941 ctactttgac atgaccgata tttacagaaa gctaggaaaa tcgagagata aaaaaacaag
     9001 aaaagatatc aaaagattga tagaagatca caaaccgctg gaaaaagagc cagccgaatc
     9061 cgtgtgatgg gtttgttccg caccgtggaa ctctcagata acggataaca acagcgtttt
     9121 ttaattattt cggatagttc tgaaaaattc cccggcagga ataaataatc agcctttctg
     9181 aaagactgga aaatccggaa atacgccagg gctgcgctcc tgagaagaac attcacgggc
     9241 gctttccaga aaagaaggat acccgtcgcg aaaagacagg aactattttc aggtattacg
     9301 cttcctggcg gattccacca ggcgccgccg ggcttcccgc ctggcccgga atgcctgttc
     9361 acgtcttttc tcctccgcca gagccgcctt tttctcggaa tcgcgtagtt tcccgtaaag
     9421 cagcgggttc atggcggcct catcttccgg gccctcatga taatacacgg gcaggccgtc
     9481 tgcagccagc gccacatggt agtaacggcc tttctccttc aagcccctgc cttttttatc
     9541 cagccgggaa agaatcacgg catatccaag cccaggggcg ttcatcagct tccgccgtcc
     9601 atcgaataat gcggccacgc gcatgttgtc gtagtccagc ttcatgacgc cgcaaaccat
     9661 ttcaccctcc gtattccagt acatgaccaa cttttttctt cccagggaat tgggcttgta
     9721 cgtttcatag gctcccttga cggggcggtt ttccacaagc agggagtaat ccgcattcct
     9781 gtaatttttt gattctctcg cggcaagctg ttctgccgga gggagcagag aggaaacggg
     9841 agcgcgcatt tccgtccgtt cctcagacag gcatttggag ggaataagaa cacccttatc
     9901 catatcataa taagcgcagc ggctcccctg ctccagaacc acatcgaaat tcttctgccc
     9961 cctgccgctt tccggacgcg cctgcggacg gaagtattcc cggatacgga acggaggccc
    10021 ctgaatgatg gaaagcgatg tggcacaaac cagtccatcc acttttctgg ttcccgttaa
    10081 agctccggca ttcttcttcc gggccatttc atgataccgg ggcgagatat tcttttctgc
    10141 cggcgtccac gtcaccttct gcagttcctt catcaggaaa ggccaaagca tcgcggccag
    10201 tttcaggttc cggcgcgcct ccgctcccgt cagagaagcc gcaagcgcag cgtcttcctc
    10261 atccagacgg gttaacggaa tttccacatg ccttcgccct attttcagaa ctgccccggc
    10321 ccctgtgcag gaatggaaac tgcccttcac cacatctcca aatttggaaa tccattcccg
    10381 ttcccccgga aaggcatatt cctccgccgg cgcacaaacc gcgccgcacc acgccagcac
    10441 agcaaataaa aggcatttca tcgtcaggaa tccaggggtg cgctttcaag ctaacatacg
    10501 ggaacagccg ggaagaagct ttttcccggc tcttttccca atcttcgttc ccggcggaat
    10561 gtttccgctg ccgttattgc ttgcgcccct ttttggcagc ccccccggat ttggagtctt
    10621 tcccggcagt tttggcggcg gtccggcccg tcttctccgc cttgccttcc gtttttccgg
    10681 cagcgttttt agcgggatat ttcctttccc gcgccgggaa ttcaaaacct attttccccg
    10741 tctttgcatt gaagaccaga taagcgtcaa acggacgctt ggtacgctgc gaaatgaacc
    10801 ccttcatcag atcggatttt ccttccgcca aaatcttcaa tacctggtcc agcgggattt
    10861 cacgtcccaa gatagtccgg gaaacggaga cggcggcatg ttcctttcca agggtcaggg
    10921 aaggaatttt ccaagccttg gccgtttcca tcacatcatg ctctcccatg tccggaatgg
    10981 cgacggaagc cactttcacg acttcctcca cccctgcggc ttcatcttcc tcccgcgcca
    11041 ggaagtaaag cgtggcccgg aatttgtcgt tgagttccag ccccgcctca aagggcttgc
    11101 cgaaccggga ggtaaatccg tggagaattc ccgtcctgcc gtccacaata agggtcttca
    11161 attcttcatc cgtcatgtcg cggccggaga taactttgcg gatgctgaac ttgcaggaac
    11221 ggcaggaaac ggcgtccagc gtgtttttca ggccgtgggc atggcaggaa gggcagtcca
    11281 attccatttc cggcagttcc acctgctgct gggcttcccg gaaatccttc accctcttca
    11341 cgatttcccc ggtcagggtc ttaatgccct ccatgaagga atccctcggc agggcggcct
    11401 gttccatctg cttgagcttg tattcccatt ccccggtcag gctgggggag gtgagcgttt
    11461 tcaggccgat gcgttccagc agggcaatca gttcaatccc gcggcgcgtc gccagaagtt
    11521 cgcggccgtc ccggacgatg tattcctggg aaatcagccc ttcaatgatg gaggcgcggg
    11581 tggcgggagt gcccaggccg cgttcgctca tggcggcagc cagttcttcg tcatgcacca
    11641 gttttcctgc cccctccatg gcagccagca gcgtggcttc gttgtaccgg gcgggcggct
    11701 tggtcatggc gttgcggatt tcaatctcac gcaaggcggc ctgctccccg tcgcggacgg
    11761 gcaccagctc ctccttggag gaagtgccgg ccttccgtcc gtaaacggcc tgccagcccg
    11821 gctccacaag cacccggccc gtggtcagga aagcgtccgt cacgccgtcc tggtgggtaa
    11881 tgagcgtggt tctccgcgtg tcttcaaaca cggcagcggg gaagaagacg gccaggaaac
    11941 gggccatgac catttccaag agtttctgcg cgtccccggt aagcttctcc atcttcccgg
    12001 tcgggatgat ggcaaagtgg tcgctcacct tttttgtatc aaacacgcgc cggttgggct
    12061 tgatgcgctt gttgtccaaa atgtccctgg caaagggggc gaattccggg ttctgctccg
    12121 ccaccgtgcc tacgatgccg taaaccttgc ccagataatc ttccggcagg tagcgggagt
    12181 ccgtacgcgg ataagtgagc accttgtgct tttcatagca ttcctgggcc agctgcagcg
    12241 tgcgcttggc ggaaaaaccg tagcggttgg cggcttcccg ctgaagggaa gtcaggtcat
    12301 acaacagcgg agcgaccgtc gtgaccggtt tcttttcttc cgtcaccgtg ccggcttttc
    12361 cacggcagcg gtcgcggatg acggccgcct gttccggatc ccagatgcgc tccgcgcggg
    12421 agtggggatg atcgtttttc ttccagtcac ggtcaatcca gcggccggag taagagccgg
    12481 actgaacgtc gaattcggcc catacttccg aatagggctc cggttcaaac gcctggattt
    12541 ccaattccct ctgcgtcaga atagccagcg tgggcgtctg cacgcgcccg gcgggggtga
    12601 cgttgaagcc gccgcgggat tgcaggatcg tcagggcgcg ggtgctgttc agccccacca
    12661 gccagtctga ttcggaacgg cagacggcgg cattcgtcag gggcttcatg tcagcttcgc
    12721 tgcgcatgcc atgccatgcg ttgaggatgg cctcatccgt catggactgc atccataagc
    12781 gccgcgtagg cttggtccat tttccgtaac gcaccagatt gtgaaagatc agttcccctt
    12841 cacgcccggc atcgcatgcg ttgacgattt ccgtgacgtc cttactcctg gcaagctgaa
    12901 gaaccttttt caaacggtct tccgactgct tgataggttc cagttcaaac ttcctgggga
    12961 tgacgggcag atagtcgaat ttccacggca gggactttcc ttcttccgtc atgggttttt
    13021 tctgttccag cagatgacct acggcagagg tgatgatggc ccggtcattc tggtaaaatg
    13081 ccccggactt gtcccgggtg aattttccaa gctctttgcc gagcactctc gccaaatcag
    13141 tggcgacgct cgnnnnnnnn nnctataatt aacgttttgg acatatcggc ggctcggaaa
    13201 aaagtgctga acaataaaag cgacgctgcc cgcacgggaa agggggcgtt gcatttggag
    13261 ctgaattcct actcccctac cccccatgct tgtcaataac caattatgta tttacgggaa
    13321 aagcgcgctt ttatgtcaaa ataacggatt ccctcatccc catttttccg aaaagccgct
    13381 ctgccacctt gccagccatc cgctgccgtg ctatttacgg gatgatgaaa actggttttt
    13441 ctttcgtcct tcttccttcc tgcctcatgc tgctggcagc ctgctcccct acaccgtccg
    13501 accgcattgc gaagaatatg ccggcttatg aacagctccc gaaggagcat caggccaaag
    13561 tggcgcgcgg ggagctggaa aaaggaatgt ctcccatggc ggttctgctg gcatgggggg
    13621 aacccgaaca gaaaatggcg ggcaggatga acgggaagaa tacggaacgg tggatttaca
    13681 cgcggagcgg cacgggctgg tccctgggcc tgggcggagg aggattccgg ggcagggatt
    13741 ccggcatagg caccgggata ggcgtcacga tgcccctggg cagccgcccg cccgtctcct
    13801 gcaatgtcct gtttgaagac gggaaggtca tcggctggga agccgtcaat ggcttttaaa
    13861 cggcgttccc cgccttgtat ttcccatctg cccggcccgg cgtttcacgc cgtccgcgcc
    13921 gttttcccta tttgccggcg gccttgacga tggcttccac cagaccgttg atatcgtgtg
    13981 ttttcgccgt aattgaaggg gtatgcccca gttccttgag ggttgccgtg gttaccgggc
    14041 cgatgctggc ggccctgcat ccttccggcc atggaagccc cagcttgaag aaattctccg
    14101 ctgtggagga actggtgaac gtcacgatgt ccgctccgcg ttcgcggaaa gccgcctggg
    14161 ctcccgccac atcctccgtt tccgcttccg ttttgtaggc gatgcattcg tccacaatgg
    14221 cgcccagcgc attcaacccg tcgtaaatca cgcggcgcgc ttcttctccg cgcacccaca
    14281 ggaatgtgct gtgctcaatg gttcctattt cttcgcgtgc gtccctgaac gctttaacga
    14341 ggccctccgc cacaaacttt ttaggcatga tatccacggc cagcccgtat tcccggagct
    14401 tcgcctccgt ccctggcccg atggccgcaa tcctggcccc tccaatgctc cggatgtccc
    14461 tgtacacggc aaaaaacgcc tggaagaagc gttccacccc gttggggctg gaaaacacca
    14521 gccagtcata tgtgtgggca tgcaccacgc tttccgcaaa ttccctcctg ttggagggtg
    14581 gggcaatgcg nnnnnnnnnn gcgtatgaca ggcatctcca gcacttccgc ccccagacgg
    14641 cgcaggcgcg cgctcaattc cccggcctgg gcacgggtgc gcgtcaccac gatgcgcttg
    14701 ccgaacagag gcagtttttc aaaccagttc aaggactcgc gttctttcac cacatccccg
    14761 atgacaacta ctgcgggagc gcccaccccg gccttttgca ccctggcggg cagtgttttc
    14821 acagttcccg taaccgtacg ctgaatgccc gtggtggccc actggacggc ggcggcaggc
    14881 acagacggtt cctgcccgta cccgatcagg gcttcgcaca catccgccag tttctgcatt
    14941 cccatcagca tgactttcgt gccctgcgct tcggctattc ccttcaaatt cagggaagac
    15001 gccttcttat tctcgccttc atggcccgta aatacggtaa actgcgtgca gtacccgcgg
    15061 tgcgtcaccg gaatgcctgc gtaagcaggc cccgcaatgg cggaagttac gcccggaaca
    15121 acttcaaaag ggatgcccgc ttcatgcagg gcgtccgcct cctccccccc gcgcccgaag
    15181 acgtaggggt cgcccccttt caggcgcacc acttttttcc cggcacggcc cagcctgacc
    15241 agcagggcgt tgatttcctc ctgcggcagg gcgtggcggg aagcccgctt gcccacaaaa
    15301 atcctttcgc atgcaacagg aacccagttc agcatttccg ccgaactcag cgcatcatat
    15361 accagcactt ccgccattcc cagcagttcc cgcccgcgga cggttatcaa cccggggtcg
    15421 ccgggacccg ctccaagcaa ataaacaata ccctgttgca tgccgccatt caactccacc
    15481 atccgctttt tggcgacaag aaaccccgcc atgcccggat tcatttcatt gatggctgaa
    15541 ttgtcacaaa acatccatat atgttgcttt tcataaaaaa tatctccccg ctataaaact
    15601 tcactgcatt ttagccttga taaggagggt atttcccatt atttaataaa attatttaac
    15661 tattttgaaa aaatatggat cctttgcatt ctccgacagt aactcttgtt gagcctacca
    15721 aaaaagaaaa aagcaccctc ttcaaaccat ctttgtatgg agggcgtatg acgagaaaac
    15781 aatactgggt acgctatttc acatttatca tgataggtag aattatatgt aatagtttca
    15841 tagaaactgc atttgacctg acagaattta ttgtctccgt aatattacta ttgttgttcg
    15901 agatattcta caccttgcct attacaataa agcgttacca tgatgctggg tattccggca
    15961 gtttagccgt atggctggcg gtaattagta caataatgtt atttcttgcc atattcacct
    16021 cgccatcatc tgctgttggt gtgtgctctt tattgatgtg catcattctt ggaattgtca
    16081 atttagttat cctttgtaag gattccgaaa tagatgacaa taaatatggt ccttccacca
    16141 agtacccgga tttggttaaa aaggaacaat aaccattccc gtcataattt cattttctat
    16201 tctatcctct gttctggaaa tgggattttt tcgatcaatc tactttgtaa atatcttctc
    16261 cgcatcaaca agaaaatcac ggacaggagt cctaaagtac cgggtggact gccggtccga
    16321 atcccccgcc gtgcgcaaag tggagcttta ttattctccg gacggcaagc tcctctctca
    16381 aaaaacggat gattgaattc gccgtttttc cgcacatttc cgtatgacag aagccggggg
    16441 actccctccg gaggctgaca cggcaccctc tggattcaac aaagcctgat gatataaggt
    16501 caaatttgca atgattgagt tattcaatat cgatctcctt tcctatacgg tacagacggc
    16561 ggaaaccgcc ccaacgcatt cctataccat ttactggttg attctgctgg gctccatgct
    16621 tctgagctgg ctggtcagcg cgcgcatgaa gagccgcttc agcgaatatt cccagatttc
    16681 cattcccgta acagggaggc aggtggcgga gcagatgctg aaggacaacc atattacgga
    16741 cgtgaaagtg atttccacgc ccggccatct gacggaccat tacaatccgg cagataaaac
    16801 cgtcaacctg agcgagtccg tttacaattc caacagcatt gccgccgctg ccgtcgccgc
    16861 ccatgaagta gggcatgccg tacagcatgc ccaggcttac cactggctgg gcttgcggtc
    16921 cgcgctggtt cccatcgtcc agctctcctc caatctggtg ggcattgtgc tgatgatcgg
    16981 cattgtgctg ctggccatgg gcggctctcc gtgggtgctg gggctgggca ttggcctgtt
    17041 tgccgtgacc actgccttcg cgttcatcac gcttcccgtg gaatttgacg cctccgccag
    17101 ggccctgaaa tggctggacc gctcccacct gatgaactat ttcgaccatg gaagggccaa
    17161 gagcgccctg ttctgggccg ccatgactta tgtggtaggc gcgctttcct ccctggccat
    17221 gctgctgtat tacgtgttca ttttcctgaa cgcccgcggc agggaataat cccgtgccct
    17281 gctgcggagg gaaaccccga ggcgttccgt ccggaactgt ccggactgcc tggcccggcc
    17341 gccagcccac aaactccact tgggcgcatg ccgcaccgcg cacagggaaa atggaaaaaa
    17401 caaatgccag cgcccccgtg aggcgatggg attttttcaa aaaagcgccg ccctgtcagg
    17461 gctttttacc tgacagggcg gtgccggttt ccggctgtat ggccagagcc gtttcatatt
    17521 ccacgtcaag aagagagcgc catccggggc cggcaaaata tcgtcatcgt cccgacgatc
    17581 gcccatcaat cagaaagggg cgtctcccgc ggcccgcgcg gaaaacgccc ctctccttac
    17641 ttccttgatg acttcaggtt attactgaag aattgttctc ggaaactccg ggccacaccg
    17701 gcattcaccc ggcaggacac ggagttccct tatgggcgga gccatttatt ggtcaatgta
    17761 aaggatcgtg ccgtccggag tcagcagaat atcgtaatcg tccagattgt ccattacctc
    17821 caccttgtaa cggatgagtt ctccctgcgt cactttgtcc gcctccctga ttctgccgcc
    17881 ggggtacggt ttcagggcgg ccgcgcgaac cggctcagga agggaagata ccgctatttc
    17941 ttccttcacc cggacaatct ttccttcagg agtcactttg acgtcaaatt caaaaccgtt
    18001 gattttgaac tcggcctcat aaagttttcc tgacccgctg gcgtcccatt caatgaagcc
    18061 ggccctgggc caggcgcctt taacggcaga gacggccgcg acgggtacgg aggattccgg
    18121 aatatccgcc catgatgaag gaatagccag cgccatgacg gcaaatatca ctttcttgcg
    18181 tatattcatg ctgaaataat aggatgatgt ttatagttga gttcctcgaa gagccttcta
    18241 aaaactccgg gccacgccgg cgttgacgcg gtggtacagg gagttccccc tcgcttccag
    18301 gctgtagcct ccgtacacgc tccagcgctc attgcactgc agggacgtgt tgtactccgc
    18361 gcggaaaccg tgccgttccg ggctcaccgc cctccccctc cagctcatgt cgctgtacgg
    18421 gctgtacacc gttccttccg gcacgtcctg caagacgtct cccacgtagg atacgcgcag
    18481 ggcctgcgcc cacggccttc ccttccattc gtccgtcctg cctatttcca gccccacggg
    18541 aatggacagg cgct
//
LOCUS       DAOMSU010000011        18344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_92_length_18344_cov_2.863853, whole genome shotgun sequence.
ACCESSION   DAOMSU010000011 DAOMSU010000000
VERSION     DAOMSU010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 18344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18344
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92_length_18344_cov_2.863853"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(609..2618)
                     /locus_tag="PK077_01085"
     CDS             complement(609..2618)
                     /locus_tag="PK077_01085"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRN22927.1"
                     /translation="MARRYVIRQDAAPVAPISGIDYRTALNEEQYAAVSSEPGPALVI
                     AGAGSGKTRTLTYRVAWLLDHGTDPWNILLLTFTNKAAREMTERVRSLIPLDLSRLWS
                     GTFHSIANRILRQHAEYLGYTPAFTIMDSDDRKSMIKSVVKSLKLDEKSTRFPKPEVL
                     SSLFSLADNEGANIRETLENAYPYLSNHLDAILEVHDQYVARKQETNSMDFDDLLLNV
                     VRLFQEQEHLRALYGSRFQHILVDEYQDTNYLQSRLIDMLAREHGQLMVVGDDAQSIY
                     SWRGADMENILSFTTRYPAAKTFKIETNYRSVPEILELSNAAIAANEVQIEKQLRSVR
                     PTGEMPPALVPLNDDRIQAKFVVQRIRDLIEEGTDPSEIAVLYRAHFHSMELQMELTR
                     GEIPFRITSGIRFFEQAHIKDVVAFMRFVVNPRDELSFRRMVMLLPGIGPGMAQKLWI
                     RWTACPESRQETPPESFSALMLDFPVPAKSRTAWTQLCYTLDELAPAGKLAGPASMLL
                     SINEAVYDEYMQAAFENYDQRRQDLLHLAGFSERFESTEDFLSQLSLLGNTDDESAAA
                     DFSGGSVTLSTIHQAKGLEWSVVFLIGLCDGMFPHQRVIDDGDLAGLEEERRLFYVGI
                     TRAKDQLYLTYPRWNCRAQGGTFMQMPSRFLDEFPAGLVEEWEVE"
     gene            complement(2701..4020)
                     /locus_tag="PK077_01090"
     CDS             complement(2701..4020)
                     /locus_tag="PK077_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRN22928.1"
                     /translation="MKNASTITAFIISFLVVGSLALVFLFLDNKEEEPAAAEPAAQTE
                     VPARQTVTPQPTVEPLTKEDISRQTATPPSLINLSSPQTLAQAIAKVIQDRDEDILKS
                     LEIQQAVTENQSRLIRELMEARHLRLSSPGITSIGANNQGANPTARYTLNFSSGARGY
                     LDMKRNDKQQWTLDTLTLPSKQDLAKDKVAPMAMNDPMGIVSSFMDAVAKADFRGARK
                     FVDGTKVQDATVAGLCILFEEGAFRLREDAPIKTAYEAPTNAGFFVHLQDAQGRKAGN
                     VGLTVAKTDGQWLVAEASLDSMLEAYTKRQGAGDDIFIPIVKNPQGGDSLALFFGFNE
                     DTLSKRSERQLQIVAEAIKMDSGKKLEISGHTDDVGSERYNQGLSERRAAAVKAQLVQ
                     FGVPAERIVTKGFGKSQPRRTYSPTADEETRDEARKENRRAEMYLDF"
     gene            complement(4045..5427)
                     /gene="mpl"
                     /locus_tag="PK077_01095"
     CDS             complement(4045..5427)
                     /gene="mpl"
                     /locus_tag="PK077_01095"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HRN22929.1"
                     /translation="MNSHFHFLGICGTAMGAVAAAMARRGFTVTGSDANVYPPMSTFL
                     EEEGITLFEGYKASNIPPDADVIVIGNAMSRGNEEVEAVLQRRLRYLSLPETMKEYFL
                     RGKRNYVVTGTHGKTTTTSMLAWLMEDSGLNPSFMIGGIARNLGRGGRFTDSDFSVLE
                     GDEYDTAFFDKRSKFLHYLPELVVINNIEFDHADIYHSLEEIKLSFRRLTQIIPRNGL
                     ALVNADDPNCLDVVKGAPCPVKTVGFSDSSNIRILDVETSTDGSHFTLEGTRYYVPMI
                     GEFNIRNAAMAIAAAQFAGLNVKQLADSMSLFEGVARRQEVKGVVNGISVVDDFAHHP
                     TAIKQAILGLRQRYPESRIWAIFEPRSNTTRRNIFQNELALSLATADFPVVAAVDHPD
                     KVALEERLDLTKLSSDLSALGKDALIGGHAQEIVSAVCKKAQPGDVILVMSNGGFEGI
                     HAKLLEALNA"
     gene            complement(5453..6337)
                     /locus_tag="PK077_01100"
     CDS             complement(5453..6337)
                     /locus_tag="PK077_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HRN22930.1"
                     /translation="MSSPQNTIALVFDYDQTLSPFYMQDQAIFPEFGISPQEFWTRCN
                     ATGEREGWDGELIYMKSLLDALSLDRVSNARLTQLGERLTFYPGLPDFFNAFPSMTLS
                     SLHTDLGISIEYYIISSGLKALIDGSALRPYFKAVFGCEFSEENGYINFPKRVISHTT
                     KTQYLFRINKGMLDYDQDVNDHMPEDMRPIPFANMIYIGDGPTDVPCFTVMRRNGGHA
                     VAVYNPEDETGRSFRKCYNLASNSDRVNFIAPADYTPGSHLTRLLSGMVTDIANGIVR
                     RRDRALADSCIAAPSFSK"
     gene            complement(6358..7104)
                     /gene="lptB"
                     /locus_tag="PK077_01105"
     CDS             complement(6358..7104)
                     /gene="lptB"
                     /locus_tag="PK077_01105"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HRN22931.1"
                     /translation="MSSFYSSPLLSAEGICKTYKTRTVVDQVSLKVGHGEIVGLLGPN
                     GAGKTTSFYMVAGLVRPDEGRVIFADEDITELPMNLRARRGMGYLPQEESIFRKLSVE
                     DNLLAILQTRKDMTSRQQKDRAVELMDRFGITKLRRSMAIQLSGGEKRRLTIARALAT
                     SPKLLMLDEPFSGVDPIAVSDIQKIVMELRDTDGLSILITDHNVRETLHIVDRAYILF
                     EGKVIKHGTSEEIANDPIARKQYLGDQFRM"
     gene            complement(7101..8060)
                     /locus_tag="PK077_01110"
     CDS             complement(7101..8060)
                     /locus_tag="PK077_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22932.1"
                     /translation="MKKLSSLLLALAGSLACSGANGVPSHIVQPLPRELDPRLEQPLT
                     PPSHIVRPLTEEETARRERELAPVPTAGLKEDGEKAIQTYSAQEKTVDGRIQEFRQQH
                     QIAGNTPAPAAETTSSSAASGPEEGFLQANSPKDLYTVDCDGDLYFDMEEGVLVFMKN
                     VRVRNPGLSLDCADHLKVYAEFVPKKPGDKKPSDIKKEKGKEGDQTQVALPNGGNFDY
                     NSIKTVAASGNVKASYTNKQGETSNAQAEKITYNAKTGEIILTGVPVIVTPDVIVKNP
                     NKDAFIRVYSNGNMYGSRGTKTTFRHVDKKLSDRENQKTSRKP"
     gene            complement(8073..>8403)
                     /locus_tag="PK077_01115"
     CDS             complement(8073..>8403)
                     /locus_tag="PK077_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22933.1"
                     /translation="PKVSLSRYEGPRLSMLLTASSVTVAAPQEIAANILNVYLYGKNN
                     QTSHLFTQAASYFLDKKLAVSHTETTLREENLSAKGTGLYLDTNIKRGIILGPVQTTI
                     RMAQRNK"
     assembly_gap    8404..8413
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8549..9364)
                     /gene="kdsA"
                     /locus_tag="PK077_01120"
     CDS             complement(8549..9364)
                     /gene="kdsA"
                     /locus_tag="PK077_01120"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HRN22934.1"
                     /translation="MSFYIQSVEIGGPRPFYLLGPCSLECESFVWEMARSIKAIAENL
                     DVPLIFKASYDKANRTSVTSFRGPGVREGCRILSEIGRELKLPVVTDVHTAQEAEIAA
                     EYVDLLQVPAFLCRQTDLLEACAKSGRPVNIKKGQFLAPWDTVNIGEKMRAFGCDEFM
                     MCERGTTFGYNNLVVDMRSLYWMKQEGFPVVFDATHAVQKPGGLGGTTGGDSELAPVL
                     ASAAMATGSIDGVFMEVHKDPSKALSDGPNQIPLHLLEGVLKRLQSIHQAVQS"
     gene            9688..10407
                     /locus_tag="PK077_01125"
     CDS             9688..10407
                     /locus_tag="PK077_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22935.1"
                     /translation="MPRKIYKLFISYDEAGAELRQHVAEQFQYSSDCVWQASFRSLTA
                     ADLPPGDGVVWVYPVFMQSGVTVTETLPELLRALYAGSGQHPELVFKPVWGAGCGGVG
                     FRAAALQKELEGEASLLVVAHGVTGREAAPEPAQFLQQLKFRLPEGTDMALAYFGAFP
                     SVEKVLPGLKGQKVVVLPFLIGKGKHMREDMPSPELAARHGKTLKILPPFGAFYLQAE
                     REYWKTGMGEPAGNGFDGPAR"
     gene            10646..>10924
                     /gene="rpsB"
                     /locus_tag="PK077_01130"
     CDS             10646..>10924
                     /gene="rpsB"
                     /locus_tag="PK077_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRN22936.1"
                     /translation="MINDLINQMVEAGVHLGHQTRKWHPSMKKYLLKDKAGIHIINLE
                     ETEKCLDKACSFLADLARRNKKILFVGCKRQAQEAVREAAEATGQYYVN"
     assembly_gap    10925..10934
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10935..11387
                     /gene="rpsB"
                     /locus_tag="PK077_01135"
     CDS             <10935..11387
                     /gene="rpsB"
                     /locus_tag="PK077_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRN22937.1"
                     /translation="EAARRSGQHFVNHRWLGGMLTNMSTIRKSIERLVYLEGIEKSPE
                     FKSMSKKELAALDRERQKLERNLQGIRNMGGAPDAMVAIGADHEDIAIREAHRLNIPV
                     CVLVDTNADPKEIDFPIPGNDDAVRSIRLILDCIVKAINEGKGASAEA"
     gene            11417..12295
                     /gene="tsf"
                     /locus_tag="PK077_01140"
     CDS             11417..12295
                     /gene="tsf"
                     /locus_tag="PK077_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRN22938.1"
                     /translation="MAEITAALVKALRDKTDAGMMDCKKALNECNGDMDAAVKYLREK
                     GIAKAAAKADRDAKEGVIRAAVAPCGCSGIILELNCETDFCAKGDKFQGLVNDVANAL
                     MDSKASTLEEALQVPMAEGSTEEYIKAMCSSIGENMALRKFARLTADGIGAVVSYIHM
                     GGKVGVLLSIKAGKEETVKSDAFKALAKDLTLHIAAAAPKSVSSDSLDPAFVAEEQEI
                     AKQQLIQEGKPEHLLEKILPGKLKALYAQSCLLNQVFVKDPAGKMTVEALLNQAGKEL
                     GDDISVASFVRYQLGA"
     gene            12829..13614
                     /locus_tag="PK077_01145"
     CDS             12829..13614
                     /locus_tag="PK077_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN22939.1"
                     /translation="MAFLELDHVSVVFPAVQGLFSARKSAPVVAVDGVSLSVEKGEIL
                     GLVGESGCGKSTLSRTVMLLQKPTEGRIFLEGEELTALSASEVRKRRPDFQMVFQDPY
                     ASLTPRFTVYATLKEALQSRRKRTFAQTRDDVAELMEKVGLSPSLMRKYPHEFSGGQR
                     QRVAIARALAPEPRLVIADEPVSALDVSIQSQILNLLIALARDLSLTMIFISHDLSVV
                     HYIADRIAVMRKGRIVEYGEADKVFSSPSHEYTQALLAAVPRL"
     assembly_gap    13872..13881
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(14134..15297)
                     /locus_tag="PK077_01150"
     CDS             complement(14134..15297)
                     /locus_tag="PK077_01150"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRN22940.1"
                     /translation="MSLHTLNIRLDFPYRTGFTHGAFRPENDALASLMEQRPGSRVLV
                     LLEKGLEQFYPQLPADIDRYFEKNAGVLAYAGCRSVPGGEEAKTGFSAWETALRHIVE
                     AGIDRHSYIIAVGGGAFLDVAGFAATTAHRGIRLLRIPTTTLSQADSGVGVKNGINFM
                     GRKNYLGTFAVAWATLDDFLFLHSQPLPLKRAGLAEVVKVAVVKDASFFNWLETNASA
                     LAVCERDALEYAVEHSALLHASHIACGGDAFELGSSRPLDFGHWAAHYMETMSGYILG
                     HAEAVSVGMCLDILYSVRKGWLPAAEAERIISVLKTLELPVFHPLLSRRTENGACEVL
                     KGLEAFREHLGGHLTVLMLTGIGRGKDVHEIDAALMEECIREMQEAAGYSVQE"
     gene            complement(15335..>16619)
                     /locus_tag="PK077_01155"
     CDS             complement(15335..>16619)
                     /locus_tag="PK077_01155"
                     /inference="COORDINATES: protein motif:HMM:NF036494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22941.1"
                     /translation="NIVYSASFVEVTSADGSQTVPVTVTAFDPEANLALLRLKNGKDA
                     AFLDKLVPVALGKAPRLGDKVTFWQFNGDGLPITTSGTLLATESACPFTNGEPFVLYN
                     VKSSVTPLKGGAGNPVMRGNELVGLSASCDPSAQKVLAVTHTMISRFLEQARAGNYTG
                     FPADGTQVTELTDPVFRKFLGLPETGGGFYVVKLPVYGSFYKAGVRPGDVVESVNGIP
                     LDSKGLIKDPALGPVSANFLFRDSAKPGDTITLGIRRKGKDGSSQPMTLDVKLDRSAL
                     EGDLVNPAPFISNPPYRIYGGLVFVPLTGALMGEINKLSKNHPPLNLVEATQKKEDIR
                     KKGVDEIVVFLMALPTQATLGYAQMSPSIVEKVNGVQVKSFKHLNQLLDLPAPGGTHR
                     IEVTQQPYTMYMSQKEAAKADRFIQMRAVPVLRRD"
     assembly_gap    16620..16637
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16638..16926)
                     /locus_tag="PK077_01160"
     CDS             complement(<16638..16926)
                     /locus_tag="PK077_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22942.1"
                     /translation="MKILSTFFFGSLILLCGCQPREAGRTAPEPQPEQQTETPAEQVE
                     ETPAPAPLPSPTGSMVGINATNQGYAMIQPWSKENPAYSQGFGIYLGDGNIL"
     gene            complement(16932..>18344)
                     /locus_tag="PK077_01165"
     CDS             complement(16932..>18344)
                     /locus_tag="PK077_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRN22943.1"
                     /translation="KIEMDSLTPDYATPWNTGRYQGGIGTGFLIGENAFMTNAHVVSN
                     AERIYISMYGDSRKIPARVKFIAHDADLALLEADDPRPFKGIRPFEFSKNLPHLEDEV
                     RVIGYPIGGNRLSVTRGVVSRIDFTTYAHPRNTEHLTIQVDAAINPGNSGGPVLMGNK
                     VIGVAFQGLNNANNTGYVIPTPVIRHFLEDIKDGVYDGYVDMGIQAAPILNPAMRKAF
                     GLPDDEKGVLIGKVLKGSSADGVLRNGDLLMKVDGYDVDSSAMIELDGQKISMKELIE
                     RCFKDDRLPLDIIRDGKPMKVEMVMKPSPSRDLLMAEYDKMPRYVVFGGLVFQPIQRN
                     VLAAADISMLDVALDIRNYQEDGGCVDHEDMVIITKVLDDEVNARLSGSISNSIVEKI
                     NGVKVKGLTHAYELLYPENMPEYVVIELKNGERPLVFEGKAMETANKRISKTYNIPKN
                     ARLDSSIPGRQSGRNTTPAR"
BASE COUNT         3754 a         4759 c         5576 g         4207 t
ORIGIN      
        1 tcttgaaccc catgaatcaa cttttactga catacaaatg tattatgtta tttttagtgt
       61 atcctgatta ctgcgtttcc cagctgaaac aggaactgag gtaatggttc catatggaga
      121 tatattcaac gttgtccgga tagatgaata tattgtttgt taataggttt catgattaat
      181 ttagaagttt ggcgagattg aaggccggga ggaatggccc gaataaatgc gggcgactca
      241 taaacctctg cgaatgaaag tctggtcggg gtattttttg acgcggcccg catatgcaga
      301 tggagcttcc ctaggagagg gccttccctt attggttttg ctggatatgg cggtgatgaa
      361 ccgcagcggt agaaacggaa ggatgcggta agagccgcgg tgtcacggtt tttggaatgt
      421 ggccggggtt atcagggaat tcccttttcc gggaaagacg gaaatatctc cggataaggg
      481 ttggaagcag gaatgcaaat gggtgggaaa agaacccttc ggaaactccg cgccgcgttt
      541 tccctgtccg gcgggagaag attgagaacc ggcgtttccc ggatacaacc cgggagagga
      601 gagctggatt attccacctc ccactcttcc accaggccgg ctgggaattc atccagaaag
      661 cgggagggca tttgcatgaa ggttcctccc tgggcgcggc agttccagcg ggggtaggtc
      721 aggtaaagct ggtccttggc gcgggtaatg cctacataaa agaggcggcg ttcttcttcc
      781 agcccggcca ggtctccgtc gtcaattacc cgctggtgcg gaaacatgcc gtcgcacagg
      841 ccgatcagga agacgacaga ccattccagt cccttggcct ggtggatagt ggacagagtg
      901 acggagccgc cggagaagtc cgccgcggcg ctttcatcat ccgtgttgcc caaaagggaa
      961 agctgggaaa gaaaatcctc cgtggattca aagcgttcgg aaaatccggc cagatgcaga
     1021 agatcctgcc tgcgctggtc gtaattttcg aatgcggcct gcatgtactc gtcgtacacg
     1081 gcctcattga tggaaaggag catggaggcc ggaccggcca gctttccggc gggggccagt
     1141 tcatccagtg tgtagcaaag ctgcgtccag gccgttctgg atttggcagg aaccgggaaa
     1201 tccaacatca gggcggaaaa ggattccgga ggggtttcct gccggctttc cgggcaggcg
     1261 gtccagcgta tccagagttt ctgggccatt ccggggccga tgccgggcag cagcattacc
     1321 atgcggcgga aggaaagttc atccctggga ttgaccacaa aacgcataaa tgccaccacg
     1381 tccttaatat gggcctgctc gaaaaacctg ataccgctgg tgatgcggaa aggtatttct
     1441 ccgcgggtca gttccatttg caattccatg ctgtggaagt gggcgcggta aagcacggct
     1501 atttcactgg ggtccgtgcc ttcttcaatg agatccctga tacgctggac gacaaatttg
     1561 gcctgtatcc ggtcgtcgtt gagcgggacg agggcggggg gcatttcccc ggtgggccgc
     1621 acggagcgga gctgtttttc aatttgaact tcgttggcgg cgatagccgc gttggaaagc
     1681 tccagaattt ccggcacact gcggtaattg gtttctatct tgaaagtctt tgccgccgga
     1741 tagcgggtag taaagctgag aatattttcc atgtccgctc cgcgccagga gtagatgctc
     1801 tgggcatcgt cccccaccac catcagctgg ccgtgttccc gggccagcat gtcaatgagg
     1861 cggctctgga ggtaattggt gtcctggtac tcatccacca gaatatgctg gaagcgggaa
     1921 ccgtacaggg cacgcaggtg ctcctgttcc tgaaaaagcc tcacgacgtt gagaagcagg
     1981 tcgtcaaagt ccatgctgtt ggtttcctgc ttgcgggcta cgtattggtc atgcacctcc
     2041 aggatggcgt ccaggtggtt ggaaaggtag ggataggcgt tttccagcgt ttcccggata
     2101 ttggctccct cgttgtctgc cagactgaac agggatgaaa gaacttccgg cttggggaag
     2161 cgggtggatt tctcatccag tttgagggat ttgaccacgc ttttaatcat ggatttgcgg
     2221 tcgtcggagt ccatgattgt gaaagccggt gtatatccca ggtattccgc atgctggcgc
     2281 aggatgcggt tggcaatgga atggaacgtg ccgctccaca ggcgggacaa gtccagggga
     2341 atcagggaac gcacccgttc cgtcatttcc cgcgccgcct tattggtgaa ggtaagcagc
     2401 agaatgttcc atgggtccgt accgtgatcc agaagccagg ccacgcgata agtcagcgtg
     2461 cgcgtttttc ccgatccggc tcctgcgatg acaagagccg gccccggttc tgaagaaacg
     2521 gcggcgtact gttcttcatt gagggccgta cgatagtcga tcccggagat gggggcgacc
     2581 ggcgcggcat cctgcctgat gacgtatcgg cgagccatgg aatatggtta ataattgaca
     2641 ggcaacagta aatagagaag ttggaaaggc ttgccccttc tctgctctct gatgactctt
     2701 ttaaaagtcc aggtacattt ccgcgcggcg gttttccttg cgggcttcat cccttgtttc
     2761 ttcgtctgcc gtgggagagt aggtccggcg cggctgagat tttccgaatc ccttggtaac
     2821 gattctttcc gcaggaacgc cgaactggac gagctgggct ttcacggcgg ccgcacggcg
     2881 ttcggagagc ccctggttgt agcgttcgct tcccacgtca tccgtatggc cgctgatttc
     2941 cagcttcttg ccactgtcca tcttgatagc ttccgctacg atttgcagtt ggcgttcgga
     3001 acgcttggaa agggtatctt cattaaagcc gaagaacagg gccagggaat cgccgccctg
     3061 gggatttttt acaatgggga taaagatgtc gtctccggcc ccctgccgtt tcgtataggc
     3121 ttccagcatg ctgtccaggg aagcttccgc aaccagccat tgtccgtccg ttttggctac
     3181 ggtcaggcct acattgccgg ccttcctgcc ctgggcgtcc tgaagatgaa cgaaaaaccc
     3241 cgcattggtg ggcgcttcgt aggcggtttt aatgggagcg tcctcccgga gccggaaagc
     3301 cccctcctca aacagaatgc acaaaccggc tactgtggca tcctgcactt tagtgccgtc
     3361 cacgaacttc ctcgccccac ggaaatccgc cttggcgacg gcatccatga aggaactgac
     3421 gatgcccatg gggtcattca tcgccatggg ggcgactttg tccttggcca gatcctgctt
     3481 gctgggcaga gtgagggtat ccagcgtcca ctgttgcttg tcattgcgct tcatatccag
     3541 atagccgcgc gcgccgctgc tgaaattgag cgtgtatctg gctgtgggat tggctccctg
     3601 gttgttcgcg ccgatgctgg tgatgccggg agaggaaagg cgtagatggc gggcttccat
     3661 gagctcccgg atgaggcggc tctggttctc cgtcacggcc tgctggattt ccagggattt
     3721 caggatgtcc tcatcccggt cctgaatgac tttggcaatt gcctgcgcca gtgtctgggg
     3781 ggagctgaga ttgatcaggg agggcggcgt agccgtctgc cggctgatat cctccttggt
     3841 aagcggctcg acggtgggct ggggcgttac cgtctggcgt gccggcactt ccgtttgcgc
     3901 cgccggttca gcggcggcgg gttcctcttc cttattgtca aggaacagga aaaccagggc
     3961 caggctgcct acgaccagga agctgatgat gaaggcggtg atggtggatg cgtttttcat
     4021 tgaaagtggg tgtatgaaga agggtcaggc gttcagtgct tccagcaatt tggcgtgaat
     4081 gccttcaaaa ccgccgttgc tcattaccag aataacgtct cccggctggg ctttcttgca
     4141 gacggcagag acgatttcct gggcatgccc gccgatcaga gcgtccttgc ccagagcgga
     4201 aaggtcgctg gaaagtttgg tgagatccag gcgttcctcc agggccacct tgtccggatg
     4261 atccacggcg gctaccacgg ggaaatccgc cgtagcgagg gacaacgcca gttcattctg
     4321 gaaaatattt ctgcgcgtgg tgttggagcg cggttcgaaa atggcccaga tgcgtgattc
     4381 cggatagcgc tggcgcaggc ccaggatggc ctgtttgatg gccgtcggat ggtgggcgaa
     4441 gtcgtccact acggaaatgc cgttgaccac gcccttgact tcctggcggc gggccacgcc
     4501 ttcaaagagg ctcatggaat ccgccaactg cttgacgttc agccccgcga attgtgcggc
     4561 ggcaatcgcc atggcggcgt tgcggatgtt gaactcccca atcatcggta catagtacct
     4621 ggttccttcc agcgtgaaat ggctgccgtc cgtggaggtc tccacatcca ggatgcggat
     4681 gttggaagag tcggaaaaac ccaccgtctt gacggggcac ggggctcctt taaccacgtc
     4741 caggcagttg ggatcgtccg cgttgaccag agccaggccg ttgcggggga tgatctgggt
     4801 cagccggcgg aagctcagct tgatttcctc cagggaatgg taaatgtccg cgtggtcgaa
     4861 ttcaatgttg ttgatgacca ccagttccgg gaggtagtgc aggaatttgg agcgtttgtc
     4921 gaaaaacgcc gtgtcgtact cgtccccctc cagcacggaa aaatcggaat ccgtaaaacg
     4981 gccgccgcgg ccaagattgc gggcaatgcc gccgatcatg aagctggggt tcagcccgga
     5041 atcctccatc agccatgcca gcatggatgt ggtggtggtt tttccgtggg tgcctgttac
     5101 cacatagttg cgcttgccgc gcaggaagta ttccttcatc gtctccggga gggaaaggta
     5161 acgcaggcgc cgttgaagca cggcttccac ttcctcattg ccgcggctca tggcattgcc
     5221 gatgacgatg acgtccgcat ccggcgggat gttggaagcc ttgtagcctt caaacagggt
     5281 gatgccttcc tcctccagaa aagtggacat ggggggatag acgttggcgt cggaaccggt
     5341 gacggtaaag ccgcggcggg ccatggcggc ggctaccgcg cccatggcgg tgccgcagat
     5401 gccgagaaag tggaaatggc tgttcatgac ggggagagga atggagcggg ttttatttgg
     5461 aaaaggaagg agccgcaata caggaatccg ccagagcccg gtcccggcgg cgcacaatgc
     5521 cgttggcgat atccgtgacc atgccggaca gcaggcgggt gagatggctg ccgggcgtat
     5581 agtccgccgg ggcgatgaag ttcacgcggt cggaattgga ggccaggtta tagcatttgc
     5641 ggaaactgcg gccggtttca tcttccggat tatatacggc tacggcgtgg cctccgttcc
     5701 ggcgcatgac ggtgaagcag ggaacgtccg tagggccgtc gccgatgtaa atcatattgg
     5761 cgaaaggaat ggggcgcatg tcttccggca tgtggtcgtt tacgtcctgg tcgtaatcca
     5821 gcattccctt gttgatgcgg aaaaggtact gggttttcgt ggtgtgggaa atgactctct
     5881 tgggaaaatt gatgtagccg ttctcctcgc tgaattcgca tccgaatacg gccttgaaat
     5941 aagggcgtag ggcggaaccg tcaatcaggg ctttcaaacc ggaggagatg atatagtatt
     6001 caatgctgat gcccagatcc gtatgaaggg aggagagggt catggacggg aacgcgttaa
     6061 aaaagtccgg caaaccgggg taaaaggtga ggcgctctcc aagctgggta agccgggcgt
     6121 tgctgacgcg gtccagggaa agggcgtcca gcaggctttt catgtaaatc agttctccgt
     6181 cccagccttc tctttctccg gtggcgttgc acctggtcca gaattcctgc ggagaaatgc
     6241 cgaattccgg gaagatggcc tgatcctgca tgtagaaggg actcagggtc tggtcgtagt
     6301 cgaagaccag ggcgatcgta ttctgcgggg aagacatggg aaagacggaa aataaagtta
     6361 catgcggaac tggtcgccca gatactgctt gcgggcgatg gggtcgttgg cgatttcctc
     6421 ggaagtgccg tgcttgatca ccttcccttc aaacaaaatg taggcacggt ccacgatgtg
     6481 gagggtttcc cgcacattat ggtccgtaat caggatggaa aggccgtccg tatcgcgcag
     6541 ttccataacg attttctgaa tgtctgaaac ggcgatggga tccacgccgc tgaacggttc
     6601 gtccagcatc agcagtttgg ggctggtagc cagggcgcgg gcgatggtca gccttctctt
     6661 ttcgccgccg gaaagctgga tggccatgga gcggcgcagc ttggtgatgc cgaagcggtc
     6721 catcagctct acggcgcggt ccttttgctg gcgggaagtc atgtctttgc gggtctgaag
     6781 aatggccaga agattatcct ccacggaaag cttgcggaaa atagattctt cctgcggcag
     6841 ataacccatg ccgcgcctgg cgcgcaggtt catcggcaat tccgtaatat cttcgtccgc
     6901 aaaaatcacg cggccttcat ccgggcgtac gagacccgct accatgtaaa aggaagtggt
     6961 ttttccggcg ccgttcggtc ccagcaggcc cacaatttcg ccatggccta ccttcaggct
     7021 gacctggtcc acaacggtcc gcgttttgta ggttttgcag atgccttcgg cgcttagcaa
     7081 aggagaagag tagaacgaac tcacggtttg cggctggttt tctggttttc ccggtcggaa
     7141 agttttttgt ccacgtggcg gaacgtcgtc ttggttccgc ggctgccgta catgtttccg
     7201 ttggagtaca cccgaatgaa agcatccttg ttcggatttt tcacgatgac gtccggagtg
     7261 acgatgaccg ggacgcccgt caggatgatt tctccggttt tggcgttgta ggtgattttt
     7321 tcagcctggg cgttgcttgt ttctccctgt ttgttggtgt aactggcttt tacgttgccg
     7381 gaagccgcga ctgttttaat ggaattgtag tcgaaattgc ctccgttggg gagagccacc
     7441 tgagtttggt ccccctcttt tcccttttcc tttttaatgt cagagggttt tttatcgccc
     7501 ggttttttcg gcacgaattc cgcgtaaact ttcagatgat ccgcacagtc cagggaaagg
     7561 ccgggattgc gcacccggac atttttcatg aacaccagaa cgccttcttc catatcgaag
     7621 tacaagtctc cgtcacaatc tacggtgtac aggtcttttg gggaattggc ctgcaggaag
     7681 ccttcctccg ggccggatgc tgcggaagag gaagtcgttt ccgcagccgg ggcaggggta
     7741 tttccggcaa tctggtgctg ctggcggaat tcctgaatcc gcccgtctac ggttttttcc
     7801 tgcgcgctgt atgtttgaat cgccttttcc ccgtcttcct tcaagcctgc ggtcggaaca
     7861 ggggccagtt cccgttccct ccgggctgtc tcttcctccg tcagggggcg tacaatatgg
     7921 gaaggcgggg tgaggggttg ttccaggcgg ggatccagtt ccctgggcag gggctggaca
     7981 atgtgggagg gaacgccgtt ggcgccgctg caggcaaggc ttccggccag ggccagcagc
     8041 agggaggaaa gttttttcat aaaaaggaat ggttacttgt tgcgttgagc catgcggatg
     8101 gtggtttgaa cgggacccag aatgatgccc ctcttgatat tggtgtccag gtaaaggccg
     8161 gtccctttgg cggaaagatt ttcttccctg agggtggttt ccgtatggga gacggccagt
     8221 tttttatcca gaaaataaga ggcggcctgc gtgaaaaggt gggaggtctg gttgtttttt
     8281 ccgtacaagt acacgttcag gatattcgcg gcaatttcct gcggggcggc cacggtcacg
     8341 cttgaagccg tcagcagcat ggaaaggcgc ggcccttcat atcgtgacag gctgactttt
     8401 gggnnnnnnn nnnctacggg cagaagctcc agcataggta attttttatc tacgccggca
     8461 aaaggcgtgg ctgcgggcag gctggtgtcc gcttgcgcct ccgtgccgta cagggcggcc
     8521 gtcaaaaagc atgtggacaa taatttcatc atgactgaac cgcctggtgg atggactgaa
     8581 ggcgtttcag cactccttca agcaggtgga gaggaatttg gttggggccg tcggaaagcg
     8641 ctttggacgg gtctttgtgc acctccatga acacgccgtc aatgctgccc gtcgccatgg
     8701 cggcggatgc cagcacgggg gccagctcgc tgtctcctcc cgtggttcct cccaatcccc
     8761 cgggtttctg aactgcgtga gtggcgtcga atacgacggg aaagccttcc tgtttcatcc
     8821 agtacaggga gcgcatgtcc accaccagat tgttataacc aaaggtggtg ccgcgttcgc
     8881 acatcatgaa ttcgtcacag ccgaaggcgc gcattttttc cccgatgttc acggtatccc
     8941 acggagccag gaactgccct tttttgatat tcacgggccg gccgctcttg gcgcaggctt
     9001 ccagaagatc cgtctggcgg cacaaaaaag cgggaacctg gagcagatcc acgtattcgg
     9061 cggcaatttc cgcttcctgg gccgtatgca catccgtaac gacgggcagc ttcagctccc
     9121 tgccgatttc cgacagaatg cggcagcctt cccgcacgcc ggggccgcga aaagaggtga
     9181 cggaagtgcg gttcgccttg tcgtaggaag ctttgaaaat cagcgggaca tccaggtttt
     9241 ccgcgatggc cttgatggag cgggccatct cccatacaaa ggattcgcat tccagagagc
     9301 aggggcccag aaggtagaac ggccgcgggc cgccgatttc aacggattga atgtaaaacg
     9361 acataaaaca aaacctgtat gtccttgtat catgacggtt tcaggccttt cggtcaagcc
     9421 gctatcctgc ctccgcgcgg agattcggag gcgaaggttt atttttccgg aagtatcttt
     9481 ttatattcga tataatgatt ttgttataaa tatggtaaat attttttaga taggtgcgtg
     9541 taaatggagg ggagcatgga ttggaaaaaa gcgtatggag ttgccgccct ccggactttt
     9601 tgttacaaac ggccatcatg cgttacttcc gcatgaataa atgacaggat gttccgaacg
     9661 ccgttccccc gcagtggtct taccgaaatg cccaggaaga tttacaaact ctttatttcc
     9721 tatgatgaag caggggctga attgcggcag catgtggcgg agcagtttca atattcaagc
     9781 gattgtgtct ggcaggcgtc tttccgcagc ctgacggcgg cggatttgcc tccgggcgat
     9841 ggtgtagtct gggtttaccc tgtcttcatg cagagcggcg tgacggtgac ggagacattg
     9901 ccggaattgc ttcgtgcgct gtatgccggg agcgggcagc atccggaact tgtttttaaa
     9961 cccgtatggg gagcgggatg cggcggtgtt ggttttaggg ccgccgcctt gcaaaaggaa
    10021 ttggaggggg aggcttccct gctggtggta gcccacgggg taacgggaag ggaggctgcc
    10081 cctgagccgg cgcaattcct ccagcagttg aaattccggc ttccggaagg aacggacatg
    10141 gcgcttgcat actttggcgc atttccttct gtggaaaagg tattacccgg attaaagggg
    10201 caaaaggtgg tagtgctgcc gttcctgatt ggaaaaggga agcatatgcg ggaggacatg
    10261 ccctctccgg aactggccgc aaggcatggg aaaacgttaa aaatcctgcc tccgttcggt
    10321 gcgttttatt tgcaagcgga acgggaatac tggaaaacgg gcatgggaga gcctgccggg
    10381 aacggttttg acgggcctgc gcgataagat attttgctga aatggcaaag ttctccatga
    10441 gcttgcttgc gacaaggttc cgcgaacggc ggaatagttt cgtaaatcaa tgatttatgc
    10501 tttgacaaga gagggggcgg gaagtatatt gcccgtccct cgcgtgattc ggcgcaacta
    10561 aacaacgggg atcggagggg taaaaagcgt cgaagtcccc aaaaactgct tttcctgccg
    10621 caaggaaagc cacctacata catatatgat taatgatctg attaaccaga tggttgaagc
    10681 cggtgttcat ttgggccacc aaacccgcaa atggcacccc agcatgaaaa agtaccttct
    10741 caaggacaag gccggtatcc atatcatcaa ccttgaggaa acggaaaaat gcctggacaa
    10801 ggcctgctcc ttcctggctg atctggcccg ccgcaataag aaaatcctct tcgtcggctg
    10861 caagcgccag gctcaggaag ccgtccgcga agctgcggaa gccactggtc agtactatgt
    10921 caacnnnnnn nnnngaggcc gcgcgccgtt cgggccagca tttcgtcaac caccgctggc
    10981 tgggcggcat gctcaccaac atgagcacaa tccgcaagag cattgaacgc cttgtctatc
    11041 tggaagggat tgaaaagagc ccggaattta agagcatgtc caagaaggaa ctggccgctc
    11101 tggaccgcga acgccagaag ctggaacgca accttcaggg catccgcaac atgggcggcg
    11161 ctccggacgc catggtggcc atcggcgccg accatgaaga catcgccatc cgcgaggccc
    11221 accgcctgaa cattcccgtc tgcgttctgg ttgataccaa cgccgacccg aaggaaatcg
    11281 acttcccgat tcccggcaac gacgacgccg tccgttccat ccgcctgatt cttgactgca
    11341 ttgtcaaggc catcaacgaa ggcaagggcg catccgccga agcctaaacc aaccatttag
    11401 aaagaacaga aacactatgg cagaaattac tgcagctctc gtcaaggccc tccgtgacaa
    11461 aacggacgcc ggcatgatgg attgcaagaa agccctcaac gaatgcaacg gcgacatgga
    11521 tgccgccgta aagtatcttc gtgagaaggg aattgccaag gctgcggcca aggccgaccg
    11581 tgacgccaag gaaggcgtga tccgcgccgc tgtggctccc tgcggctgct ccggcatcat
    11641 ccttgaactc aactgtgaaa cggacttctg cgccaagggg gacaagttcc agggtctggt
    11701 gaatgacgtt gccaacgctc tcatggattc caaggcctcc acgctggaag aggctctcca
    11761 agtccccatg gcggaaggct ccacggaaga atatatcaag gccatgtgca gctccattgg
    11821 cgaaaacatg gctctgcgca aattcgcccg cctgacggcg gacggcatcg gtgccgtggt
    11881 ctcctacatc cacatgggcg gcaaagtggg cgttctgctg tccatcaagg ccggcaagga
    11941 agaaaccgtc aagtctgatg cgttcaaagc cctcgccaag gatttgacct tgcacatcgc
    12001 cgccgccgct cccaagagcg tcagctccga tagcctggat cccgccttcg tggccgagga
    12061 acaggaaatc gccaagcagc agctcattca ggaaggcaag cccgaacacc tgctggaaaa
    12121 gattcttccc ggcaagctga aggctctgta cgcccagtcc tgcctgctga accaggtatt
    12181 tgtgaaggac cctgccggaa agatgacggt ggaggccctc ctcaaccagg ccggcaagga
    12241 actcggcgac gatatctccg tcgcctcctt cgtccgctac cagctcggtg cgtaagctct
    12301 gagtcagagt atctctggaa ccgttccctt tgcggggaac ggttcttttg tttgatatgg
    12361 ctccatgggg gtatttatcc ataaatttga ttcagggagg atccggaaat ccggataggg
    12421 gatcatcaaa atatgcggga tagaaaagct ccggccctgc tgctggttgc ggccttcatc
    12481 ctggcagccg tttttggagc gcttttgggc tggagcatgt tttttccttg tgtgcgatcc
    12541 ctgatatttc ctggcggtat tgtggttctt caaaaaggcc aatggacggt ggaaatgggg
    12601 gaacagcctc ttttgctgtt atcagcctcg tgtgtgatga tgaactcttc tggctccggg
    12661 tggctccgga agacatgcga aatgcccagc cggggctggc gttcattgcc ggtcctggtt
    12721 tttctgtggg ggaaacggca tgggaggtgc gcatggatgt cctgatattg gaggaactgg
    12781 cgcggctggg gtcttgtctt gcatcggaag gcttgttagg gtggcggcat ggcttttctg
    12841 gaactggatc atgtgagcgt tgtctttcct gcggtgcagg ggctgttctc tgcccgcaaa
    12901 tctgcccccg tagtggcggt ggacggggtg tcgctgtccg tggaaaaggg agaaatcctg
    12961 gggctggtgg gtgaatccgg ttgcggcaaa tccacgctgt ctcgcacggt gatgctgctc
    13021 cagaagccta cggagggccg catttttctg gaaggggaag agttgacggc tctttccgca
    13081 tccgaagtca ggaagcgccg tccggatttc cagatggttt tccaggaccc ttatgcttcc
    13141 ctgacccccc gttttacggt atatgcaaca ttaaaggagg cgttgcagag ccgccgtaaa
    13201 agaacttttg cccagacgag ggatgatgtg gcggaactga tggaaaaggt agggctgagc
    13261 ccctccctga tgcgcaagta tccccatgaa ttttccggag gccagcgcca gcgcgtcgcc
    13321 attgcgcgcg cattggcgcc ggaacccagg ctggttatcg cggatgaacc cgtttccgcg
    13381 ctggacgttt ccatccagtc ccagattctg aaccttctta ttgccctggc ccgcgacttg
    13441 agcctgacca tgatattcat ttcccatgat ttgtccgtgg tccattacat cgcggaccgc
    13501 attgccgtga tgcgcaaagg ccgcattgtg gaatacgggg aggcggacaa agtattttct
    13561 tccccttccc acgaatatac gcaggcgctt ctcgccgccg taccgcgtct gtaaatgggg
    13621 atggcccggc gtcagtttgg acgaatacgg gaaagttccg tttcccgcgg agttatccgc
    13681 acaggtttaa aaggggacgg ttttccggaa gaacctccct gaattttcaa cggaacgcgg
    13741 ttcccggcag tttggttttc ccgtgccttg caaagacgat gaatggattt gtttccagcg
    13801 gggcatcgtg tccactgcct ccgcatggtt gacggcatga tcttctcttt gaggtatccg
    13861 gcaggctgtt cnnnnnnnnn nttttgaaag aggagaagta aatccggcgc gttatgaaaa
    13921 aagggggagt cgcaaaatgg cttgcatcat gggctgcccg gcagcacata ataatgccgc
    13981 cttgcggaaa ctgccggaac agaaggatat ggagattgtg ggaaggaaaa acgtcgttgt
    14041 ttttttcata ctgccgtccg gctggtattt ctctgtgata cgccagatgg cttctgcctg
    14101 ttttctgacg gggaggcagc aaggaaaggg ctgttattcc tgaacggaat atccggcggc
    14161 ttcctgcatt tcccggatgc attcctccat cagagcggcg tctatctcat ggacgtcttt
    14221 tccccggcct attccggtaa gcatcagtac cgtcaggtgg ccgccgaggt gttcgcggaa
    14281 ggcctccaga cctttcagca cttcgcaggc gccgttttct gtcctgcggg aaagcagggg
    14341 gtggaatacg ggcagttcca gggttttcag cacggagatg attctttccg cctccgcggc
    14401 aggcagccat cctttccgga cggaataaag gatatccagg cacatgccca cggaaacggc
    14461 ctccgcgtgc cccagtatgt aaccggacat ggtttccatg taatgggcgg cccaatgtcc
    14521 gaagtccagg ggacggctgg aacccagttc aaaggcgtct cctccgcatg caatgtgcga
    14581 ggcgtgcagc agagcggaat gctccacggc atattccaga gcatcccgtt cgcatacagc
    14641 cagcgcggag gcgttcgttt ccagccagtt gaagaaagag gcgtctttca ccaccgccac
    14701 tttcactact tccgccagcc cggctctttt cagggggagg ggctgggaat ggaggaacag
    14761 gaagtcgtca agcgtggccc acgcgacggc gaaggtgccc agatagtttt tccggcccat
    14821 gaaattgatc ccgtttttga cacctactcc ggagtctgcc tgggagaggg tggtggtggg
    14881 gatgcgcaac agccggatgc cgcgatgggc ggtggtggcg gcaaagcctg ctacgtcaag
    14941 aaacgctccg ccgcccacgg ctatgatata ggaatggcgg tcaatgcctg cttccacaat
    15001 gtggcgcagg gccgtttccc atgcggaaaa acctgttttg gcttcctccc cgcccggaac
    15061 ggaacggcag ccggcatagg ccagcacccc ggcatttttt tcaaaatagc ggtcaatgtc
    15121 tgccggaagc tgaggataaa actgttccag ccctttttcc aggagaacga gaacgcggct
    15181 tccgggcctc tgttccatca ggctggccag agcatcgttt tccgggcgga aggctccatg
    15241 ggtaaagcct gtcctgtagg gaaaatcaag ccggatattc agcgtgtgaa gtgacatgcc
    15301 aggagggctg caagtgaaca tggctcgcgg attcctagtc cctgcggagc acgggaacgg
    15361 ccctcatctg gatgaatcgg tccgctttgg cagcttcctt ctgggacatg tacatggtgt
    15421 acggctgctg ggtcacttcg atgcggtgcg tgccgccggg agcgggaagg tccagaagct
    15481 ggttgaggtg cttgaagctt ttcacctgca cgccgttgac tttttccaca atggaggggc
    15541 tcatctgggc gtatcccagt gtagcctggg tgggcagggc catcaggaag accacgattt
    15601 catccacgcc ttttttccgt atgtcctctt tcttttgagt ggcttccacc aggttgaggg
    15661 ggggatggtt cttgctgagc ttgttgattt ctcccatcag ggctcccgtc agcgggacaa
    15721 ataccagacc tccgtaaatg cggtagggcg gattggagat gaagggggcc ggattgacca
    15781 agtccccttc aagggcgctc ctgtccagtt tgacgtccag cgtcatgggc tggctggagc
    15841 cgtcctttcc cttgcggcgg atgcccagcg taatggtatc ccccggcttg gcggagtctc
    15901 ggaacagaaa gttggcggaa acggggccca gggcgggatc cttaattaaa cctttgctgt
    15961 ccagagggat gccgttgacg ctttccacca cgtctccggg acgtactcca gctttgtaga
    16021 aggagccgta aacaggcagt ttcaccacgt aaaagccgcc gccagtttca ggcaggccca
    16081 ggaatttgcg gaagacgggg tcggtcagtt ccgtgacctg ggtgccgtcc gccgggaaac
    16141 cggtgtaatt gccggcccgg gcttgttcca ggaaccggga aatcatggta tgggttacgg
    16201 ccagcacttt ctgtgcggag ggatcgcagc tggcgctgag gcccacaagt tcattgcccc
    16261 tcatgacggg gttgcctgcg ccgcctttca ggggagtgac ggaggatttg acgttataca
    16321 ggacgaacgg ttccccgttc gtgaacgggc aggcgctttc cgtcgccaga agggttccgg
    16381 aggtagtgat gggaaggccg tcgccattga attgccagaa ggttaccttg tcgcccaggc
    16441 ggggagcctt ccccaatgca acgggaacta gtttgtccag aaaagcggca tcttttccgt
    16501 ttttcaggcg cagaagggca agattggctt ccgggtcaaa ggcggttacg gtcacgggaa
    16561 ccgtctggga gccgtccgcg gaggtcactt ccacgaagct ggcggaataa acgatgttgn
    16621 nnnnnnnnnn nnnnnnntca ggatattgcc gtctcccaga taaatgccga agccctgact
    16681 gtacgccggg ttttccttgc tccacggctg aatcatggca tagccctggt tggtggcgtt
    16741 aatgccgacc atggagccgg tgggggaggg aagcggggcc ggcgcgggcg tttcctccac
    16801 ctgttccgca ggcgtttccg tctgttgttc aggctggggt tcgggagcgg ttcgtccggc
    16861 ttcgcgcggc tggcaaccgc agagcaatat cagggaaccg aagaaaaaag tggaaagaat
    16921 tttcatggcg gttaacgggc gggagtggtg ttgcggccag actggcgacc gggaatggag
    16981 ctgtccaggc gggcgttttt cgggatattg taggttttgg agatgcgctt gttggccgtc
    17041 tccatggcct tgccttcaaa gacgaggggg cgctctccgt ttttcagttc gatgacgacg
    17101 tattccggca tgttctccgg gtaaaggagt tcgtaggcgt gggtaaggcc tttaaccttt
    17161 acgccgttga ttttttccac aatggaattg gaaatggagc cggacagacg cgcgtttacc
    17221 tcatcatcca gcactttggt gatgatgacc atgtcttcat ggtccacgca gccgccgtct
    17281 tcctggtaat tccggatgtc cagtgcaacg tccagcatgg agatgtccgc cgccgcgagc
    17341 acgttgcgct ggatgggctg gaacaccagg cctccaaaga cgacgtagcg gggcatttta
    17401 tcgtattccg ccatcagaag gtctctggag ggggatggct tcatcaccat ttccaccttc
    17461 atgggcttgc cgtcgcgaat gatatccagc gggagccggt cgtctttaaa acagcgctca
    17521 atgagttctt tcatgctgat tttctggccg tccagttcaa tcatggcgga actgtccaca
    17581 tcatagccgt ccactttcat cagcaggtcc ccgttgcgga gcacgccgtc ggcggaggac
    17641 cccttgagca ccttgccgat caggacgcct ttttcatcgt ccggcaggcc gaacgccttg
    17701 cgcatggctg ggttcaggat gggcgcagcc tgaatgccca tgtccacgta accgtcataa
    17761 acaccgtcct tgatgtcttc cagaaagtgg cggatgacgg gggttggaat cacgtacccc
    17821 gtgttattgg cgttgttcag gccctggaat gccacgccga tgactttatt ccccatcagg
    17881 acggggccgc cgctgttgcc cgggttaatg gcggcgtcca cctggatggt caggtgttcc
    17941 gtgttgcggg ggtgggcgta cgtggtgaaa tcaatgcggg agaccacgcc gcgcgtgacg
    18001 gaaagtctgt tgccgccgat ggggtagccg atcaccctta cttcgtcttc cagatgaggc
    18061 aggtttttgc tgaattcaaa aggccgtatt cctttgaagg gcctgggatc gtccgcctcc
    18121 agcagagcaa gatccgcgtc atgggcgatg aatttgaccc tggcgggaat tttacgggaa
    18181 tctccgtaca tggagatata aatgcgctcc gcgttgctaa ccacatgggc gtttgtcatg
    18241 aaagcgtttt ccccgatcag gaaaccggtg ccgatgcctc cctggtagcg gccggtattc
    18301 cacggagtag cgtaatccgg agtcagggaa tccatctcaa tttt
//
LOCUS       DAOMSU010000012        17906 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_98_length_17906_cov_3.290964, whole genome shotgun sequence.
ACCESSION   DAOMSU010000012 DAOMSU010000000
VERSION     DAOMSU010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 17906)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17906)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17906
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98_length_17906_cov_3.290964"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..467)
                     /gene="dtd"
                     /locus_tag="PK077_01170"
     CDS             complement(15..467)
                     /gene="dtd"
                     /locus_tag="PK077_01170"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRN22944.1"
                     /translation="MRLVIQRVSKAAVHIGGVCAGAVGPGLLILAGIEEEDTEEDVSW
                     LANKAAAMRIFSDADGKMNLSVREVGGSALVVSQFTLHASTRKGNRPSFIRAARPERA
                     IPLYELFKKELASLLEGRVESGEFGADMQVSLINDGPVTIFMDSRKRE"
     gene            complement(464..1225)
                     /locus_tag="PK077_01175"
     CDS             complement(464..1225)
                     /locus_tag="PK077_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22945.1"
                     /translation="MTSSAKVFFSLLAAAFCAQGILADGENYSLWPRRPEALAEARRL
                     MDRGSLPKALELLQPLAEQGGVVGKEAKELIGRLRIRQLLDPNGPDVKKYTVRKGDSW
                     IRMVRKLGCSQAMVVHLNGLMDIPALHTGDVFKYRPLDFHVVVNVPEKEICLYDGTGF
                     VKGYPILSMKDGGKKNVETTVKDEQAPVSIYSRQFPSADKTLVLAAGGYVIDAARGTP
                     RSPGFYLSRQDCNELAMLTRPGTKVTILRGKGEEP"
     gene            complement(1197..1766)
                     /locus_tag="PK077_01180"
     CDS             complement(1197..1766)
                     /locus_tag="PK077_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22946.1"
                     /translation="MESPSAFSSMSDLPSFSRDDIRYAAEMTRVVYEPDRRIDTFGDT
                     RFNFLLLSELMDEVNVVRVRSGWVEAEKPKIIRPSIYNEISTEGFSGEAKRFFDWLSK
                     NGPGFLTLLEYGFRFKRSEVSEEILHEPLDSVRGRLLDQVRNGEDTFMALIEGVDDAW
                     EVSLLKFTVEMIQKSHEINIFDFKRKGLL"
     gene            complement(1835..2077)
                     /locus_tag="PK077_01185"
     CDS             complement(1835..2077)
                     /locus_tag="PK077_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRN22947.1"
                     /translation="MSDNSIEEKVRSIIVDQLGVESDKVTADAKFIEDLGADSLDTVE
                     LVMAFEENFDIEVPDEEAEKLQSVADVVAYIEKVQG"
     gene            complement(2209..2937)
                     /locus_tag="PK077_01190"
     CDS             complement(2209..2937)
                     /locus_tag="PK077_01190"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRN22948.1"
                     /translation="MTYLLIDNSNTRTKFVLSSPEALLPERYMIPTREVSGERLDEVL
                     AGVRYDAAVVCSVVPRVAEELKNWIVKPCHFLSCDSRLGVGIDYPHPRQIGADRLANA
                     VGAAAYYGYPCVVVDFGTAVTFDVIGPQCTYVGGVIAPGLASMGDYLERNTALLPAID
                     PQEPSRVIGTSTVEAMQSGAVYGYRGLVKEILARLEGELGVRPAVVATGGDAALIARG
                     VERIDHVDPDITLNGLRIAAGLNL"
     gene            complement(2943..4070)
                     /locus_tag="PK077_01195"
     CDS             complement(2943..4070)
                     /locus_tag="PK077_01195"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22949.1"
                     /translation="MQKGRLPRILIVTAGYGEGHNSAARGVRDALAGRAEVLVTDLCA
                     EAMPRMFGLTRAAYLWMISRMPRLWKLIYEVSDRRNMAEKPVRGIAPVERLLERLLRE
                     WKPDAVVCTYMVYPYMLDSLASRTDRAVPYLTVVTDSFVINKSWLCSKSPLWAVTDPW
                     TRAIMEEKGLPQDRLRVTGFPVNPVLGALAKEHPLSWKEGEPFRVLYFAQRSARHARA
                     ELAGMLDANPALHVTCILGRRFRRIYPRIRDLRARYGRRLTVRGWTRRVPSYLAASHV
                     VVGKAGGATVHEVLAAARPMLVNFLLPGQEEGNTRLLEKLGGGGHVPDVRALASALQE
                     MMADGGAQWRRMHENLLRAGMTGGSGRIADLALKLAEEHTN"
     gene            4214..5224
                     /gene="fmt"
                     /locus_tag="PK077_01200"
     CDS             4214..5224
                     /gene="fmt"
                     /locus_tag="PK077_01200"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRN22950.1"
                     /translation="MIPFSKPDLTLTCAGRQGKLPAMRIVFMGTGDIAIPAFRSLIRH
                     SDLAGLVTQPDRPVGRHQVLTAPAIKNIAREAGIPVLQPHSLRSPDALSNLRRLNPDL
                     IVVMAYGQILSQEVIDMAPMGCINAHASLLPRHRGAACIQSAIKSGDAETGITIMHIV
                     RKLDAGDIIAQISTPLEGTETGGSLHDKLARMTPDVLLPVIHSIEKGTATRIRQQEIL
                     ATYAPKLLRADGKIDWTRPAEEIGRMIRAYDPWPGTFTNYWNRKKRIRNMKIFPGFSI
                     LPEAEGKPGQVLSAGEQGLLIACGSGGLLVTDVQLEGSTRMNISQLIAGHPNLKDIHF
                     DV"
     gene            5225..7231
                     /locus_tag="PK077_01205"
     CDS             5225..7231
                     /locus_tag="PK077_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRN22951.1"
                     /translation="MAKSFSMESLNAAQRKAVQTLQGPVLILAGAGTGKTRTVTCRIA
                     HMVDRGISPKSILAVTFTNKAALEMRERVGQMVERKAARQIMVSTFHSLCVRILREDI
                     GRLGYKTNFTIYSGSEQSGLIRRLIVRHGGITEKITPKDVLSSMSRMKNSGLGLDSIE
                     DNLTANIAASYQRELQAQNSVDFDDLLILADKLLKEHRDVREAWQQRFRYITVDEFQD
                     TNSLQMSLLGHLVGPEHNVCVVGDDDQSIYGWRGAQISNILEFERFFPNPSIIKLEEN
                     YRCTAPILDTANALIRHNLGRRDKTLRAHKGGGEAVRLISMPGDAEEAEFIITDIENV
                     RRQEGRPWEDFAILFRANTQSRIIEQTLREHKIPYRMVGAQSFFDRKEVKDLISYLAT
                     IENPQADEYLLRILNTPPRGISELTSHLAIDWSREHGNSVWAALQDEEFLAALSTRAR
                     NSVQAFNELIAKYIDLFQDKETDFGDILEQLIEETGFSEYVTRLCKTEAEIQKRLVSI
                     GDVKASLRNFWQPGKTLRDYLAQVTLDKEDNDDDVENKPGVCLITMHAAKGLEFPVVY
                     LVGLEEGILPHKRSLEDGNCDEERRLLYVGITRAQEKLMLTYCATRLRYGDRMPCQRS
                     SFLSEIPPHLMEYSKWEDLMNAEATEEESGNFFDSLRSMLLEEE"
     assembly_gap    7365..7374
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7627..9915)
                     /locus_tag="PK077_01210"
     CDS             complement(7627..9915)
                     /locus_tag="PK077_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420017.1"
                     /note="Members of the Solute:Sodium Symporter (SSS), TC
                     2.A.21 as described in tcdb.org, catalyze solute:Na+
                     symport. Known solutes for members of the family include
                     sugars, amino acids, nucleosides, inositols, vitamins,
                     urea or anions, depending on the system; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/solute symporter"
                     /protein_id="HRN22952.1"
                     /translation="MMKTLATIFGALALMTWGASAQEGPVPATAADAQPALAAPAATP
                     AVEVQPAAEIQPAAPAAEAVQTASAEPKTAPLSMVEVILFCVVVVGVIALGIWKSRDP
                     EETEEEKKAKGASDYFLAGRGLTWWLVGFSLIAANISTEQFVGMSGKSANWVGMAIAG
                     YEWLAAITLVVVAFCFLPKLLKGGVYTIPEFLEYRYNTLARSLMAIATLLILVGVPTA
                     GVIYAGAKVISVFFTGYSAMGIDFGNITVGCVIIAFCATVYVFVGGLKACAWTDLFWG
                     AALIVGGGVVAYFALTELSGADPNHLIQSAAANSGATVASLGNPSDSLWHGVTRFFEL
                     NSGDAASGVNTVGGKLHMIRPADDAEIPWTALCLGLWIPNFFYWGLNQYIMQRTLASK
                     SLAEGQMGIVFAAFLKLIIPFVVVVPGILAYNLYRNDLKEQAEVKYAAEIRKTEDPAA
                     VKGRPVIYKLTDSFLVENVEEGCAHAIHNAEVMKVGEDVMANLKQACADLKADAANDQ
                     TTLAERAPFVEKIASLNNKIIKPAVDNSDNYYLTDTLVGFDYDSAFGTLIRKLLPGTG
                     WTWFVLAALFGAVVSSLASMLNSASTIFTMDIYNKLRKNAGPTELVTVGKIGLLVCAV
                     IALTIAPFLDSPAFGGIFNFIQEFQGFLSPGALCVFLFGFFVPKCPRIFGWLGIVINA
                     LLYGILKVWQPEMAFLNRMAVCFITVVVIGFIFTAVNAARGGQPIVLPDRGVVALQSS
                     SRAKIFGWLVVAATVALYIIFW"
     gene            complement(9925..11103)
                     /gene="galK"
                     /locus_tag="PK077_01215"
     CDS             complement(9925..11103)
                     /gene="galK"
                     /locus_tag="PK077_01215"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRN22953.1"
                     /translation="MDLVQREISKETVTPYFIEYFGQAPTHVAAAPGRVNLIGEHTDY
                     NNGFVMPMALDNHCVVAVAPSPVGKHRFCGSLGDQIHEIAVEDALVPGEPFWSNYVRG
                     VLANLHRRGIEIGPVDMLIDSNVPRGGGLSSSAALEVAVCTALAAFAGVEIDPKEVAL
                     IGQAVEHEFVNVPCGIMDQFISANGKKGMALKLDCATLEYELVPMNNESVSVLVLDSA
                     VKHSLADGAYGQRRKQCEEASSIMGVPSLREATLELLESFREQLGDVRYRRARHVIGE
                     NARVNAFANALARGDWDEAGVAMRGSHASLRDDYEVSCAEVDTLVSLCDRIPSASSIY
                     GARMTGGGFGGCIVALVKTEDVEKVAQELLDSYCQETGIETTYLVTRAGEGARILYQA
                     "
     gene            complement(11322..>12531)
                     /locus_tag="PK077_01220"
     CDS             complement(11322..>12531)
                     /locus_tag="PK077_01220"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRN22954.1"
                     /translation="SLLLYVCCDQFGVYYRCQTLWESIRRFVVAYACFVVLSVMIWKF
                     FVPFSADVGVQTLAYLLTFLGSLWLRISRFRAEKRLVEEAPTLVVGTPDHVKEFRRTL
                     ENNHVDFRNGLLVMAPEEVDSAVVRNLLIKNCVSKVVFLPEEVDSSVARCLVELCGKM
                     GVDFYASMVVSMPAVHKTYFGVIGGTRMLVYKSTPIPYTTSWQLKKMLDWTGALALLV
                     GTSPLWVLAAVGIKLSDRGPVFYRQKRSGLYGREFGMWKFRTMYRDADKRLDEVKAKY
                     GNDMDGPIFKLEHDPRIFSFGRFLRKFSIDELPQLINVLKGEMSLVGPRPLPVYETEA
                     FTSDAHRRRLSVLPGVTGYWQIAGRSNIREFEKLVELDMKYIDNWSLWLDIKLLLKTV
                     PAVLFARGAK"
     assembly_gap    12532..12541
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12542..12817)
                     /locus_tag="PK077_01225"
     CDS             complement(<12542..12817)
                     /locus_tag="PK077_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22955.1"
                     /translation="MPANLHILSMLIQNADRTTYEINKIVVFVLAFVVFFVVGFVSWQ
                     WDGVAPEPWRLRTLVLTPLTMSNRGRCPLSDQAFSTPTLNAPPAPPPD"
     gene            13192..13857
                     /locus_tag="PK077_01230"
     CDS             13192..13857
                     /locus_tag="PK077_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22956.1"
                     /translation="MSQHNTQGRQGQGARRRYNKGGRSYGNDRRDSSGYRPKYRKEAP
                     AKLSLWQRFLGLFGIRPSKNNKYAARDKAAAKTNTRVARNRQDRVSAPKQPVSNRRLY
                     VGNLSYEATESDLEDVFKGIGEVNSVEIIYNPRTHKSKGYAFVEMKKMEDAIRSVDIL
                     HNQPFMGRNLLVSGANERQEQPRQPREDRAPREEQGSMAEVKETSTPLLQPEAPAPQS
                     QEQ"
     gene            complement(14064..14798)
                     /locus_tag="PK077_01235"
     CDS             complement(14064..14798)
                     /locus_tag="PK077_01235"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRN22957.1"
                     /translation="MKAVYLCLCMLAAFCFPAAALPADCSQVIVGSTDGWNSSHVQLS
                     LLEKGPRGWVMVKGPFPARLGKSGLVWGRGVSLPPAGGPVKKEGDLRSPAGIFELGGV
                     YGTVPAPQKKRSMPYRRITPRDMWVDDPASPLYNQHFVLKHDPVTPWEFKQQMKLNDY
                     AHSLKLFIRHNAADGRRKPVPGAGSSIFFHIWRRDGGAPTAGCTAMSEENLRAMIAWL
                     DPSRHPLYILLPAREYLRLRRAWGLP"
     gene            complement(14887..16773)
                     /locus_tag="PK077_01240"
     CDS             complement(14887..16773)
                     /locus_tag="PK077_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22958.1"
                     /translation="MKCCFLMTLACAVPALGQMMYNPPAAGGAPAAPPASPPAVQPNA
                     YQPSRQGDAKSLYGNELPFLNPQDGTVTINGTTLNMGSFREIEARFNKYLSQPEESTE
                     DAKEYQKIFEKLHETLSMRKEKLAADNVVRQVVDLLTAASSNPLDGGVSDALCQAIYT
                     AWQARSNGKNKGKMLEAVEREIRSNAQKMSLMESGVTAISGAPSGQKGGKKGTPSNHP
                     AKNNPRYKYLEKRVVEMEARKLKLESEQVLTVTEAKVVFQSTLVQLFAQRRFDHVSIG
                     CGVYSRLFNDGDTKLRLDKNSDAAKMFSGTLGAPPTVAILDNLSRELARDSDRHMKAV
                     NNLVDSRHYVDALERLNEALLIGEFMPAVNTFPYEKKQKLYAFKRDVEKLFELMNGKD
                     YEEALSLVEALKKTSRDFSTGRAESAISAAVFASDAYISQGQEALARGDRAKLEECLK
                     SAIEIWPKNPRLLPLRNAMMAAGQQSHALEDFKRFHKSKNYRRIFDNQHEFAVLVKDD
                     PELQKQFVEDLGKMAVIERALGAARQREAMQDVYGAWEELQQLRSKDQELFINDQELN
                     ARYLDLTTKASTLVNLLNDAEKCRNEGEVGSALGKYMEAKRLYLYSRFAKEGIESLLD
                     EIFR"
     gene            complement(16791..17222)
                     /locus_tag="PK077_01245"
     CDS             complement(16791..17222)
                     /locus_tag="PK077_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22959.1"
                     /translation="MSRRRAAKKKNSSSSALVLTVGAAVFVLAVIVGIVMFRAGEKPQ
                     AGSDIPLDILAANASQLSNNNYALDGTVTDRFPRGESELIAFLYKDASGRECIIPVCV
                     SAEARNGLNINRDQQYRIEFRVEDVNPKVRGVCVATSIQPK"
     gene            complement(17276..>17906)
                     /locus_tag="PK077_01250"
     CDS             complement(17276..>17906)
                     /locus_tag="PK077_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN22960.1"
                     /translation="LNMKTGSAVSGIIMFEVILLGLMGSNNAAREVAGERAVMEKEKY
                     AGMRPSAYLASKLSYLSVLVLVQSVWMFAFVDFFWDRGGGLTHLLFLILANAAMTFVC
                     LGISALARSADQASLLSIYLVGFQLPLSGAVLALPEYVEDFIRPFISAYWAWSGSISA
                     LKDDVYHAVKNVLDTDLTPSLVCYIVLGVHVACGIAAAYAGIRRSRWEL"
BASE COUNT         4100 a         5531 c         4731 g         3524 t
ORIGIN      
        1 aaagaccctc cccatcactc tcttttcctg gagtccatga aaatggtcac cggaccgtcg
       61 ttaatcaggg aaacctgcat atccgcgccg aattcgccgc tttccaccct tccttccagc
      121 aaagacgcca gttccttctt aaacagctca tacaggggaa tggcccgctc aggcctcgcg
      181 gcgcggatga aagagggcct gttccctttc cgggtggagg catgcagcgt aaactggctc
      241 accaccaacg cgcttccacc cacctccctt acggaaagat tcatttttcc gtccgcgtcg
      301 gaaaaaatgc gcatggccgc cgccttgttg gcgagccagc tcacatcttc ttccgtatcc
      361 tcctcttcaa tgcccgccaa aatcagcaga ccgggcccca cggcccccgc gcaaacaccg
      421 ccgatatgaa cggcggcctt actgacgcgt tgaataacca gcctcacggt tcctctcctt
      481 tccccctcag gatagtcacc ttcgtccccg gcctggtcag catggccagt tcattgcagt
      541 cctgccgact cagataaaac cccggcgagc ggggcgtgcc gcgggcggcg tcaatgacat
      601 agcctcctgc cgccagcacc agcgtcttat ccgcagaagg aaactgcctg ctgtaaatgg
      661 acacgggggc ctgctcatcc tttacggtcg tttccacatt cttttttccc ccgtccttca
      721 tggagaggat gggatatccc ttgacaaaac ccgtcccgtc gtaaaggcag atttcctttt
      781 ccggcacgtt caccaccacg tgaaaatcca ggggccggta cttgaacaca tctcctgtgt
      841 gaagagcggg aatatccatc aaaccgttca gatgaaccac catggcctgg gagcaaccca
      901 gcttcctgac catgcgtatc caggaatctc ccttccgcac ggtatatttc ttcacatccg
      961 gcccattggg atccagcaac tggcggatgc gcagcctccc gatgagttcc ttcgcctctt
     1021 tccccaccac gccgccctgt tccgccaggg gctgcaacag ttccagagct ttcggcaggc
     1081 tgccgcggtc catcaggcgg cgggcctctg caagggcctc cggacggcgc ggccagaggg
     1141 aataattttc tccgtccgcc aaaattccct gggcacagaa agccgccgcc agaaggctaa
     1201 agaagacctt tgcgcttgaa gtcaaagata ttgatttcat gggatttttg tatcatttcc
     1261 accgtgaatt tcaagaggga gacctcccat gcgtcatcca ctccctcaat gagagccatg
     1321 aacgtatcct ctccattgcg cacctggtcc agcagccggc cacgcacgga atcaagcggt
     1381 tcatgcagaa tttcctcgct cacttccgac cttttgaatc tgaatccgta ttccagcagc
     1441 gtcagaaagc ccgggccgtt tttggagagc cagtcaaaaa agcgcttggc ttctccggaa
     1501 aatccctccg tactgatttc attgtaaatg gaaggccgta tgattttcgg tttctccgct
     1561 tccacccagc cgctgcgcac gcgcaccacg ttcacctcat ccatcagttc actgaggaga
     1621 agaaaattaa acctggtatc gccaaaagta tcaatgcggc ggtccggttc atacaccacc
     1681 cgcgtcatct ccgccgcata gcggatatcg tcacgggaaa aggaaggcag gtcgctcatg
     1741 gaagaaaagg cagaggggct ttccatgaaa aagcggcttc cttgccttgg acagggaagc
     1801 cgcttttgta ataaaggatt tttagcagtt taacttaacc ctgaaccttt tcgatgtaag
     1861 caacgacgtc ggccacagac tgaagctttt cagcttcttc atcgggcact tcaatgtcaa
     1921 agttttcttc gaaagccatc acgagttcaa cggtatccag cgaatcagca ccgagatcct
     1981 cgatgaattt cgcatcagca gtcactttat cggattcaac gccaagttgg tcgacgatga
     2041 tacttcttac tttttcttcg atgctgttgt cagacatgat atttattgtt aaggttcatt
     2101 gatattacat ggggtatgcg ccatttgcaa cacctattta tgagttttta cgcgtcatcc
     2161 ggccacggcg ccggaagcag caggtttcca gccgattttg gccgttcttt aaagattcag
     2221 cccggcggct atgcgcaagc cgttcaacgt aatgtcaggg tccacatggt caatgcgctc
     2281 cacgcccctg gcgatcagcg ctgcatcccc tcccgtagca accacggccg gacgcacgcc
     2341 cagctcacct tccagccggg ccagaatttc cttcaccagc ccccggtaac cgtacacggc
     2401 gccggattgc atggcctcta ctgtgctggt tccaatgacg cgggacggct cctgggggtc
     2461 aatggcggga agaagcgccg tattgcgctc caggtaatcc cccatgctgg caagccccgg
     2521 ggcaatgacg ccgcccacat aggtgcattg gggaccgatc acgtcaaaag tcacggcggt
     2581 gccgaagtcc accacaacgc acggataccc gtaataagcc gcggcgccta cggcgttcgc
     2641 cagcctgtcg gcccctatct ggcgcgggtg gggatagtcg atgcccacgc ccagccggga
     2701 atcgcaggaa aggaaatggc acggtttgac gatccagttt ttcagctctt ccgccacgcg
     2761 cggcacaacg gaacacacca cggcggcatc ataacggacg ccggccagca cctcgtccag
     2821 ccgctccccg ctcacctccc gggtgggaat catataccgc tccggcaaca gggcttccgg
     2881 cgaagaaagc acgaacttgg tgcgcgtgtt ggaattgtca atcagaagat aggtcacggg
     2941 aatcagttag tatgttcctc cgccagtttc aaggccaaat ccgctattct gccgcttcct
     3001 ccggtcatcc ccgcccgcag caggttttca tgcatgcgcc tccactgcgc gccgccgtcc
     3061 gccatcattt cctgaagagc ggaagccagg gcgcggacat ccggcacatg gccgccgccg
     3121 cccagttttt ccagaaggcg ggtattgccc tcctcctggc ccggaagaag aaagttcacc
     3181 agcatgggcc gtgcggccgc aagcacttcg tgcacggtgg cgcctcccgc tttcccgacc
     3241 actacatggc tggcggcaag ataggaaggc acgcggcgcg tccagccgcg caccgtcagc
     3301 ctgcgtccgt atctggctcg caaatcccgg atgcgaggat aaatgcgccg gaaccggcgc
     3361 cccagaatac aggttacatg cagggcggga ttcgcatcca gcatacccgc cagctccgcc
     3421 cgcgcatgcc gtgcggaacg ctgggcaaag taaagcaccc ggaacggctc tccttccttc
     3481 caggaaagag gatgctcctt cgccaacgcc cccagcacgg gattgacggg aaatcccgta
     3541 acgcgcagcc tgtcctgcgg cagccccttt tcctccatga tggccctcgt ccagggatcc
     3601 gttacggccc agagagggga cttggaacac agccatgatt tattgatgac gaaggaatcc
     3661 gttacgacgg tcaggtaggg gaccgccctg tccgtgcggg aggccaggga atccagcatg
     3721 tagggatata ccatgtaggt gcagaccacg gcatccggct tccattcccg cagcaaacgt
     3781 tccagcaggc gctccacggg ggcgattccc ctgacgggct tttccgccat attgcgcctg
     3841 tcgctcacct catatatcag tttccagagg cggggcatgc gggaaatcat ccacaggtag
     3901 gcggcgcgcg tcaggccaaa catccggggc atcgcctccg cgcacaggtc cgtaacgagg
     3961 acttccgcgc gccctgcgag agcatccctc acgcccctgg cggcggaatt gtgcccttcc
     4021 ccgtatcctg cggtcacaat cagaatgcgc ggcagccgtc ctttctgcat agaacagggt
     4081 ataccaatat tccgctccat cgaaaagaac ttcttgcagg agggcagaca tgaggggaag
     4141 cggccggaaa aacattatcc tccggtattc cctccggacg gaaaggaacg ggaaaaaacg
     4201 gcctttcttc cctatgattc ccttctcaaa accggattta acattgacct gcgcaggccg
     4261 tcagggcaag cttccagcca tgcgcatcgt cttcatgggc acaggcgaca ttgccattcc
     4321 cgccttccgc agcctgatcc gtcattcgga tctggcaggc ctggtcacgc aacccgaccg
     4381 ccctgtcggc aggcaccagg tgcttacagc cccggccatc aaaaatattg ccagggaagc
     4441 gggcattccc gtgctccagc cgcattccct gcgcagcccg gatgcgctga gcaacctcag
     4501 gagactgaat ccggatctaa tcgtcgtcat ggcgtacggg cagattctga gccaggaagt
     4561 gattgatatg gcccccatgg gctgcatcaa cgcccatgcc tccctcctgc cgcgccatcg
     4621 cggagcggcc tgcatccagt ccgccatcaa atccggcgat gcagaaacgg gcatcaccat
     4681 catgcacatt gtcaggaagc tggatgccgg agacatcatc gcccagatca gcacccccct
     4741 ggaaggcacg gaaaccggag gttccctgca tgacaagctg gccaggatga cgccggatgt
     4801 gctgctgccc gtcatccatt ccattgaaaa aggaacggct acacgcatcc gccagcagga
     4861 aatcctggca acatacgccc ccaagctcct gcgggcggac ggaaaaatcg actggacgcg
     4921 ccccgcagaa gaaatcggcc gcatgatccg ggcgtacgac ccgtggcccg ggaccttcac
     4981 caactactgg aaccgcaaga aacgcatccg caacatgaaa attttccccg gcttttccat
     5041 cctcccggaa gcggagggaa aaccgggcca ggtgctctcc gccggagaac aggggctttt
     5101 gatcgcctgc ggaagcggag gattgctggt gacagacgtc cagcttgaag gcagcacgcg
     5161 catgaatatc tcccagctca ttgccggcca cccgaatttg aaagatatcc attttgacgt
     5221 ataaatggcc aagtcctttt ccatggagag cctgaacgcg gcccagagga aggccgtgca
     5281 gaccctgcag gggcccgtgc tgatcctggc cggggccgga acgggtaaaa cgcgcaccgt
     5341 cacctgccgc atcgcccata tggtggaccg gggtatcagc cccaaaagca tcctggccgt
     5401 gacgttcaca aacaaggccg ctctggaaat gcgggaacgc gtaggccaga tggtggaacg
     5461 caaggcggcg cgccaaataa tggtgagcac tttccattcc ctctgcgtgc gcatcctgag
     5521 agaggatata gggcgcctgg ggtacaagac caatttcacc atttacagcg gttccgaaca
     5581 aagcggcctg atccggcgac tgattgtgcg ccacggcggc attacggaga aaatcacgcc
     5641 caaggatgtg ctttcctcca tgagccggat gaaaaacagc ggtctgggcc ttgactccat
     5701 tgaagacaac ctgacggcca acatcgccgc ctcctaccag cgggaacttc aggcgcagaa
     5761 ttccgtggat tttgacgacc tgctgattct ggcggacaaa ttgctgaagg agcaccggga
     5821 cgtcagggag gcatggcaac agcgcttccg ctatatcacc gtggatgaat tccaggatac
     5881 caacagcctc caaatgagcc tgctgggcca tctggtgggg ccggaacaca atgtctgcgt
     5941 agtgggggac gacgaccagt ccatctacgg ctggcgcggg gcccagatca gcaatatcct
     6001 ggaattcgaa cgtttttttc caaatccctc catcatcaaa ctggaagaaa attaccgctg
     6061 caccgccccc atcctggaca cggccaatgc cctgatccgc cataacctgg gccggaggga
     6121 caagacgctg cgcgcccata aaggcggagg agaagccgtc cggctcattt ccatgcccgg
     6181 agacgcggaa gaagcggaat tcatcatcac ggacattgaa aacgtccgca ggcaggaagg
     6241 ccggccttgg gaggatttcg ccattctgtt ccgggccaat acccagagcc gcatcattga
     6301 acaaacgctc cgggaacaca aaattcccta ccgcatggtg ggcgcgcaaa gttttttcga
     6361 ccgcaaggag gtaaaggacc tcatttccta tctggcgact atcgaaaacc cgcaggctga
     6421 cgaatatctg ctgcgcatcc tcaacacgcc gccgcgcggc atcagcgagc tgaccagcca
     6481 tctggccatc gactggagcc gggagcacgg caacagcgtc tgggcagccc tccaggacga
     6541 agaattcctg gcggcccttt ccacccgcgc ccgcaacagc gtgcaggcat tcaatgaact
     6601 gattgcaaaa tacatcgacc ttttccagga taaggaaacc gacttcggag acatcctgga
     6661 gcagcttatt gaggaaaccg gattcagcga atacgtcacg cgcctgtgca aaacggaagc
     6721 tgaaatccag aaacggctgg tgtccatcgg ggacgtgaaa gcctccctcc gcaacttctg
     6781 gcaacccgga aaaaccctcc gggactacct ggcccaggtc accctggaca aggaagataa
     6841 cgatgacgac gtggaaaaca agccaggcgt atgcctgatt accatgcacg ccgccaaggg
     6901 actggaattt ccggtagtgt acctcgtggg gctggaggaa ggcatccttc cccacaagcg
     6961 ttccctggaa gacggcaatt gcgatgaaga acgccgcctg ctttacgtag gcatcacgcg
     7021 cgcccaggag aaactcatgc ttacttattg cgccacgcgc ctgcgttacg gggaccgcat
     7081 gccttgccag cgctcctcct tcctcagtga aattcctccc cacctgatgg aatattccaa
     7141 atgggaggat ctcatgaacg cggaagcgac ggaagaggaa tccggaaact ttttcgattc
     7201 cctgcgcagc atgcttctgg aagaggaata agaggcggca tggcgttttg ccggcctctt
     7261 ttcaaccatg tattttattc agagaacaag gcgccgtttc cgtccgggaa cgccagcctg
     7321 ccggctttcc gcctccagaa tattcagccg cgccagctgc atggnnnnnn nnnnccctgc
     7381 gcagcatgct tctggaagag gaataagagg cggcatggcg ttttgccagc ctcttttcaa
     7441 ccatgtcttt tactttaact ttattcagag gacaaggcgc cgttgccgcc cggaaacgcc
     7501 agtctgccga ctttccgcct ccagaatatt cagccgcgcc agctgcatgg tctgttccgg
     7561 acgtgaaaaa tcctcccggc ctgaaccggg aggattgaaa atacgggact ggaggtcttc
     7621 cctggattac cagaagatga tgtacagggc aaccgtcgcg gcaaccacga gccatccgaa
     7681 gattttagcc cgtgaagagg actgaagggc aaccacgccc ctgtcgggca gcacgatagg
     7741 ctgtccgccg cgggcggcgt tcaccgccgt gaagatgaag ccgatgacca ctaccgtgat
     7801 gaaacacacg gccatgcggt tcaggaaggc catttccggc tgccacacct tcaggattcc
     7861 gtacaggagg gcattaatga cgatacccag ccaaccgaag atgcgcgggc acttgggcac
     7921 aaagaagccg aagaggaaca cgcacagggc gcccgggctc aggaagccct ggaattcctg
     7981 aatgaaattg aagatgccgc caaaggccgg gctgtccagg aacggagcaa tggtcagggc
     8041 gatcacggcg cacaccagca aaccaatctt gccgacggta accagctccg tgggccccgc
     8101 atttttgcgc agcttgttgt aaatatccat cgtaaagatg gtggacgcgg aattcagcat
     8161 ggatgccagg gaagacacca ccgcgccaaa gagagccgca agcacaaacc atgtccatcc
     8221 cgtgccggga agcaacttcc tgatcagcgt gccgaaggcg gagtcatagt caaagcctac
     8281 cagcgtatcc gtcagatagt agttgtccga gttgtccacg gccggcttga tgattttatt
     8341 gttaagggaa gcgatttttt ccacgaacgg ggcacgttcc gctaacgtgg tctggtcgtt
     8401 ggcggcatcc gctttcaaat cggcgcaggc ctgtttcaaa ttggccataa catcttcgcc
     8461 caccttcatt acttccgcgt tatgaatggc atgggcacag ccttcctcca cgttttccac
     8521 caggaagctg tccgtaagct tgtagatgac ggggcggccc ttgactgcgg cgggatcttc
     8581 cgtcttacgg atttccgccg catattttac ttccgcctgt tccttcaggt cattgcggta
     8641 caggttatag gccaggatgc cggggaccac caccacgaat gggatgatga gcttgaggaa
     8701 cgcggcgaac acaatgccca tctgaccttc cgccagggat ttggaagcca gcgtacgctg
     8761 catgatgtac tggttgaggc cccagtaaaa gaagttggga atccacaggc ccaggcaaag
     8821 agccgtccac gggatttccg catcatccgc aggacggatc atgtgcagct tgccgcccac
     8881 ggtatttaca ccgctggccg cgtcaccgga gttcagctca aaaaagcgcg tcaccccatg
     8941 ccagaggctg tccgaaggat tccccaggga ggcaaccgtc gcgccggaat tggcggcggc
     9001 ggattgaatc aaatggttcg ggtctgcgcc gctgagctcc gtcagggcaa aataggctac
     9061 cacgccgccg cccacaatca gggcggcgcc ccagaacagg tccgtccagg cgcaggcttt
     9121 caacccgccc acgaacacgt ataccgtggc gcagaaggca atgataacgc agccgacggt
     9181 gatgttgccg aagtcaatgc ccatggcaga gtatcccgtg aagaacacgg agatcacctt
     9241 ggcgccggcg taaatcacgc ccgcagtcgg cacgcctacc agaatcagca gcgtggcaat
     9301 ggccatcagg gaacgggcca gcgtgttata ccggtattcc aggaattcgg gaatcgtata
     9361 tacgccgcct ttcagcagct tgggaaggaa acagaaggct acaaccacca gcgtgattgc
     9421 ggccagccat tcatacccgg cgatggccat gcccacccag ttggccgatt tgccggacat
     9481 ccccacaaac tgttccgtgg aaatgttggc ggcaatcagg gaaaaaccca ccagccacca
     9541 ggtcagaccg cggccggcca agaaatagtc tgaagcgccc ttggccttct tttcctcttc
     9601 cgtttcttcg gggtcacggc ttttccagat gcccagggcg attacgccca ccacgaccac
     9661 gcagaagaga atgacttcca ccatggaaag aggggccgtt ttaggctccg cagaggcggt
     9721 ctgtacagcc tctgcagcgg gagccgccgg ctgaatttcc gctgccggct ggacttcaac
     9781 agcgggagtc gcggccggag cggccagggc gggctgcgca tcagctgccg ttgcaggcac
     9841 gggcccctcc tgggcggatg cgccccacgt catcagggcg agcgccccga atatagttgc
     9901 tagtgttttc atcatcagta tggattaagc ttggtacaga atacgggcgc cttctccggc
     9961 acgggttaca agatacgtag tttcaatgcc cgtttcctgg cagtagctgt ccagaagctc
    10021 ctgggccacc ttttccacgt cctccgtctt caccagggcc acaatgcatc cgccaaaccc
    10081 gccgcccgtc atgcgcgcgc cgtaaatgga ggatgcggag ggaatgcggt cacaaaggga
    10141 aacaagggta tccacctcag cacaggaaac ttcatagtcg tcacgcaggg aagcatggct
    10201 gccgcgcatg gctacaccgg cctcatccca atcgccgcgg gcaagggcat tcgcaaaagc
    10261 gttcacgcgc gcattttctc caatgacgtg gcgggcgcgg cgatagcgca catcgccaag
    10321 ctgttccctg aaggattcca acagctccag cgtagcttcc cgcagggagg gcacgcccat
    10381 gatggaagaa gcttcctcac actgcttgcg gcgctgtcca taagctccgt ccgccaggga
    10441 atgcttcaca gcgctgtcca gaaccagcac ggagacggac tcattgttca tcggaaccag
    10501 ctcatattcc agcgtggcgc aatccagctt gagagccatg cccttcttgc cgttggcaga
    10561 aataaactgg tccatgatgc cgcagggaac gttcacgaat tcatgttcca cggcctgccc
    10621 gatcagggct acttccttgg gatctatttc aacgccggca aaagcggcga gcgccgtaca
    10681 gacggcaact tcaagagcgg cactggagga gaggccgccg ccgcggggca cattgctgtc
    10741 aatcagcata tccacaggcc cgatttctat gccgcgcctg tgcaggttgg ccaaaacgcc
    10801 gcggacataa ttggaccaga acggttcgcc gggaaccaag gcatcttcca ccgcaatttc
    10861 atggatctgg tcacccaggg aaccgcaaaa gcggtgtttg cccacggggg agggagccac
    10921 agccacaaca caatggttat caagcgccat aggcatcaca aaaccgttat tataatccgt
    10981 gtgctcacca ataaggttca cacgtcccgg tgccgctgcc acatgagtag gcgcctgacc
    11041 gaaatactcg atgaaatacg gagtcaccgt ttctttagag atttctcgct gaactaaatc
    11101 cataatagat tgattctgcc ccatcccttt ccgcacctca agattaaagt ctctgcccgg
    11161 caataatttt cttcattttc cacaaaatac accaatttca tccccctccc gaattgcagt
    11221 attcttaaaa cgggcctgtt ccaccggaca gagaacccca gccagattcc aaattcccgg
    11281 caataacggc tgacggcctg caatccaggg agacctggaa gctactttgc cccacgggcg
    11341 aaaagcactg ccgggacggt tttcagaagc agtttgatat ccagccacag ggaccagttg
    11401 tcaatatact tcatatccaa ttccaccagc ttttcaaatt cccggatgtt gctgcgtccg
    11461 gcaatctgcc agtatcccgt cacaccgggc agcacgctca acctgcggcg gtgggcgtcg
    11521 ctggtaaagg cttccgtctc ataaacgggc agcgggcgcg ggccaaccag gctcatttcc
    11581 cccttcagca cattaatgag ctggggaagc tcgtcgatgc tgaatttgcg caggaagcgc
    11641 ccaaaggaga agatgcgagg gtcgtgctcc agcttaaaga tggggccgtc catgtcattg
    11701 ccgtacttgg ctttcacttc atccagcctc ttatccgcgt cccggtacat ggtgcggaac
    11761 ttccacatgc caaactcccg accatacagc ccggaacgtt tctggcggta aaagaccggg
    11821 ccacgatccg acagcttgat tcccacagcc gccagcaccc acaggggtga cgttcccacc
    11881 agaagcgcca gcgctcccgt ccagtccagc attttcttca actgccagga ggtggtatag
    11941 ggaatggggg tggatttgta caccagcatt cttgttcctc caatcacgcc gaaataggtt
    12001 ttatgcacag cgggcatgct taccaccatg ctcgcgtaaa aatcgactcc catcttgccg
    12061 cacagttcca ccaggcaccg cgccacggaa gaatccacct cctccggcag aaacaccacc
    12121 ttgctgacgc aattcttaat caacaaattc ctgactaccg cactgtccac ctcttcaggc
    12181 gccatgacca gcagcccatt cctgaagtcc acatgattgt tctccagcgt cctgcggaat
    12241 tctttcacat gatccggagt tcccaccacc agcgtagggg cttcctccac cagccttttt
    12301 tccgcgcgga accggctgat acgcagccag agggaaccca gaaaggtaag cagatacgcc
    12361 agggtctgga caccgacatc cgcggagaag ggaacaaaga acttccatat catcacgctt
    12421 aacaccacaa aacaggcata tgccaccaca aaccgccgga tgctttccca gagcgtctgg
    12481 cagcggtagt aaacgccgaa ctggtcacag cacacataca gcagcagaga cnnnnnnnnn
    12541 ngtcgggcgg cggcgcaggc ggcgcgttca gcgttggcgt gctgaaggcc tggtcggaca
    12601 aggggcaacg cccgcggttc gacatcgtca gcggcgtcag caccagcgtc cgcagacgcc
    12661 acggttccgg agcgacccca tcccattgcc aggacacaaa tccgacaacg aagaagacga
    12721 cgaacgcaag gacgaaaaca acaatcttat taatctcgta ggtagtacgg tctgcatttt
    12781 gaatcagcat cgagagaata tgtagattag ccggcattta aaatgctccg tccttgtctt
    12841 tttgtcaaat tgtttgctca ctctgagcca tgcccggcac ggaagcctgc ctcccggacg
    12901 gccttcccgc ctccgggcga gcagaaagcc taaaatgacg agataccggc ttgcaagaat
    12961 ccggctccct gtgcattggt atacgcgtca atcgaccgga ctggtcacgc gcctagcctg
    13021 atcgcccccc tatcagacca tgcatggacc gttgatcggg gcatggggcc cccttactcc
    13081 tggcttttct catggcagcg gaacgtcccg gcgatcctcc ggatggaatt tactgcacac
    13141 agcgataaat ccggcatgac accaacataa tcaccaactt agcatataag tatgtcacaa
    13201 cacaacacac agggaagaca gggccaggga gctcgccgcc gctacaacaa gggcggacgc
    13261 tcctatggca atgaccgccg cgattccagc ggttaccgtc ccaagtacag aaaagaagcg
    13321 cccgccaaac tctccttgtg gcagcgtttc ctgggcctct tcggcattcg tccgtccaag
    13381 aataacaagt atgcggccag ggataaagcc gccgccaaaa ccaacacccg cgtagcccgc
    13441 aacaggcagg atcgcgtttc cgcacccaaa cagcccgtct ccaaccgccg gctttatgtg
    13501 ggcaatcttt cctatgaagc tacggaaagc gatctggagg acgtgttcaa gggcatcggg
    13561 gaagtcaatt ccgtggaaat catttacaac ccccgcaccc acaagtccaa ggggtatgct
    13621 tttgtggaaa tgaagaagat ggaagacgcc atccgttccg tggacattct ccacaaccag
    13681 cctttcatgg gccgcaatct gctggtcagc ggagccaatg aacgccagga acaaccccgt
    13741 cagccccgcg aagaccgcgc acccagggaa gaacagggtt ccatggcgga agtgaaggaa
    13801 acctccactc ccctgctcca gccggaagct cccgctcccc agtcccagga acaataacca
    13861 ttttaccatg cggaagccgg cctttcttca aaggccggct tcctgcattt acgggaaagg
    13921 gaactcctgg cagcaacgaa cgtgcagcac ggcacgggag ccgcaccatc caccagccct
    13981 tccatccgta gtgtttcaac cactcccctg tccgtcccgg ggcggcggaa caggcgtgcc
    14041 tgcaaaggcg gattctgaaa atatcacggc agcccccagg ctctgcgcag gcgcaaatat
    14101 tccctggcgg ggagcagaat ataaagggga tgtctggaag gatccagcca tgcaatcatg
    14161 gccctcaaat tttcctcgct cattgcggtg catccggccg taggagctcc tccgtcccgg
    14221 cgccagatat ggaaaaaaat ggaggaaccc gcacccggca cgggtttccg ccgtccgtct
    14281 gcggcattat gccggataaa cagtttcaga ctgtgggcgt aatcattcag cttcatctgc
    14341 tgcttgaact cccacggcgt cacgggatca tgcttcagta caaagtgctg gttatacagg
    14401 ggagaggcag gatcatccac ccacatgtcc cgcggcgtaa tgcggcggta cggcatggaa
    14461 cgctttttct gcggcgcggg gacggttcca tatacgcctc ccagttcaaa aatgcccgcg
    14521 ggagaacgca agtccccctc cttcttcacc ggccctccgg cgggagggag gctgacgccc
    14581 ctgccccaga ccagcccgga ttttcccaga cgcgccggaa acggaccctt caccatcacc
    14641 cagccgcgcg gccctttctc caaaaggctc aactgcacat gggaactatt ccacccgtcc
    14701 gtggaaccta caatcacctg gctgcaatcc gccggaagag ccgcagccgg aaaacaaaac
    14761 gccgccagca tgcacaaaca caggtatact gccttcatga gccgtagcat aacggcaccg
    14821 ttccggaaga aaagcaatta ctccgtctcc acaggcacgc catgcggcgg aatttatccg
    14881 tccagttcaa cggaaaattt catcaagcag ggactcaatt ccctccttgg caaaccggga
    14941 atacagatac agccttttag cttccatgta cttgcccagg gcggacccca cttctccctc
    15001 attcctgcat ttttccgcat cattcagcaa attcaccagc gtgcttgcct ttgtcgtcag
    15061 gtccagatac ctggcgttta gctcctggtc attgatgaac agctcctggt ctttggaacg
    15121 cagctgctga agctcctccc aggcgccgta tacgtcctgc atggcttcac gctgacgcgc
    15181 cgcgcccagg gcacgctcaa taacggccat tttgcccaaa tcttccacaa actgcttctg
    15241 gagctccgga tcatccttca ccagaacggc aaattcatgc tggttgtcaa agatgcggcg
    15301 ataattctta ctcttgtgga aacgtttgaa atcctccagc gcatgggatt gctgacccgc
    15361 ggccatcatg gcgttccgca gcggaagaag gcgggggttt ttcggccaga tttcaatggc
    15421 gcttttcagg cattcctcca gtttggcgcg gtcacccctg gccagggctt cctgaccctg
    15481 ggaaatatag gcgtcactgg cgaatacggc ggcgctgatt gcggattccg cacggcccgt
    15541 gctgaaatcc ctggaagtct tcttaagagc ctccaccagg gaaagggctt cttcataatc
    15601 tttgccgttc atcagctcga acaacttttc cacgtcccgc ttgaaggcat acagcttttg
    15661 cttcttctca tacggaaacg tatttacggc aggcatgaat tccccgatca gaagggcttc
    15721 gttcagccgt tccagcgcat ccacataatg acgggaatcc accagattgt tcacggcctt
    15781 catgtgccgg tcggagtcac gggccagctc ccgggaaaga ttgtccagaa tagcgacggt
    15841 gggcggagcg cccagcgtgc cgctgaacat cttggcggca tcggaatttt tatccagccg
    15901 cagcttggta tccccgtcat tgaacaggcg gctgtaaacg ccgcaaccta tggaaacatg
    15961 gtcaaaacgc cgctgggcaa acaattgaac cagcgtagac tggaaaacaa ctttcgcttc
    16021 cgtcaccgtc agcacctgtt cactctccag cttcagcttg cgggcttcca tctccaccac
    16081 acgcttttcc aggtatttgt aacggggatt atttttcgcc ggatgattgg agggagtccc
    16141 ctttttcccg cccttctggc cggagggtgc accggaaatc gccgtcacgc cgctttccat
    16201 caggctcatc ttctgggcgt tgctgcggat ttccctctcc accgcctcaa gcatcttccc
    16261 cttgtttttc ccattgctcc tggcctgcca cgcggtgtaa atggcctggc agagagcgtc
    16321 ggaaacgcct ccgtccagcg gattgctgga agcggcggtc aacaggtcca ctacctgccg
    16381 caccacatta tccgccgcca gtttttcctt gcgcatggag agcgtttcgt ggagcttttc
    16441 aaaaattttc tgatattcct tcgcatcttc cgtgctctcc tccggctggc tcaggtattt
    16501 gttgaaacgc gcttcaatct cccggaaact gcccatattc aacgtcgttc cgttgatggt
    16561 caccgtgccg tcctgagggt tcaggaaagg cagctcattg ccgtacaggg acttggcatc
    16621 cccctgcctg gaaggctggt aggcattcgg ctgaacggcg ggaggggagg caggaggcgc
    16681 ggcaggcgca ccgccggctg caggagggtt gtacatcatc tgccccagcg caggaaccgc
    16741 gcaggccaga gtcatcaaaa aacagcattt cataggacga gggagattgt ttatttaggc
    16801 tgaatggatg tagctacgca cacgccgcgc accttcggat tgacgtcttc cacccggaac
    16861 tctatcctgt attgctggtc ccggttgatg ttcagtccgt tcctggcctc agcagaaaca
    16921 cagacgggaa tgatgcactc acgcccggag gcgtctttgt acaagaaggc aataagctcg
    16981 gactcgccgc gcggaaaacg gtcagtcacc gttccgtcca gtgcatagtt gttattggaa
    17041 agctgggagg cgttcgccgc cagaatatcc agaggtatat ccgacccggc ctgaggcttc
    17101 tccccggctc ggaacatgac aatccccaca ataacggcca gcacaaaaac cgccgccccg
    17161 accgtcaaaa ccagtgcgga tgatgaggaa tttttctttt ttgctgcacg ccgtctcgac
    17221 ataatttttg atggaagaag tatataacgg acttcccgca aatgcacgca ttaaattaaa
    17281 gctcccagcg cgaacggcgg attcccgcgt aggcggccgc aatgccgcac gcaacatgca
    17341 cgcccagaac aatataacaa accagggagg gcgtcaggtc cgtatccagc acattcttga
    17401 cggcgtgata tacgtcgtcc ttcagggcgg agatgctgcc ggaccacgcc caatacgcgg
    17461 aaatgaaggg ccgtatgaaa tcctccacat attccggaag cgccagcacc gctccggaga
    17521 gaggaagctg gaaacccacc agataaatgc tcagcaggga agcctggtcc gcactccggg
    17581 ccagggcgga aatgcccagg catacaaaag tcatggcggc gttcgccaaa atcaggaaca
    17641 gcagatgcgt caggccgccg ccacggtccc agaaaaaatc cacaaaggca aacatccaga
    17701 cggattgcac cagcaccagg acgctcagat aggacagctt gctcgccagg taggcggaag
    17761 gcctcatgcc cgcatacttt tccttttcca tgacggcgcg ttcgccggcc acctcgcggg
    17821 cggcattgtt ggatcccatc agccccagca gaatcacctc aaacatgatg atgccggaaa
    17881 cggcggaacc ggtcttcatg ttcaac
//
LOCUS       DAOMSU010000013        17633 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_100_length_17633_cov_3.016953, whole genome shotgun sequence.
ACCESSION   DAOMSU010000013 DAOMSU010000000
VERSION     DAOMSU010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 17633)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17633)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17633
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100_length_17633_cov_3.016953"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="PK077_01255"
     CDS             <1..1076
                     /locus_tag="PK077_01255"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRN22961.1"
                     /translation="PLSAQGQSIVDSRASLPRAVQLHGDEGRETVRPDESKRISPNEA
                     PASRVQPQRIVNRIAATVNGRPITANEVSVRLMPIGAQLAAQYPKQGPEFYKQLALAK
                     KNIIEDLVERELLRNEFEGMGGVIRDSLIDQEVNRTILTTFNGDRSAFLKNLSLSGMT
                     IRAFREMTKKQLQVQIMRASKYDQEIPPTPEEIQQEYESTKEQYRDLTKDKIKFKKIF
                     IPMLGDDSASTPEVQLNLAELIAKEIKSKNATFEEMAKRYSKDLYAEKGGDWPVTERS
                     TLSPESAAIIFGAQPGEIIGPLVDSTGFTIVLVEKKELAPPPPLSAIKEQIDIMARNK
                     RSNERYKKWVERLRKKAIVKIYI"
     gene            1373..>3495
                     /locus_tag="PK077_01260"
     CDS             1373..>3495
                     /locus_tag="PK077_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRN22962.1"
                     /translation="MFTNITRTTLCITAFSIASLMAAPLNATKTESLDWNWKFARFGK
                     MPDGSTQPEPGKAMGFATATSEESGNPADNAVDGDKSTRWCAASGKSGEKITVDMGRP
                     VDVKTVNILWEKQSNHLFKLEGSGDGKRWATIEDKTSGQNDSKEDTVENKTGKPRYFR
                     ITVTGNNQSNWASIREITFKNDKGEIIRPQAAAGTSKSDNPSSPSFNDKNWRSLNLPH
                     DWGVEGPFRMEIENRTGKLPWVGIGWYRKTLEIPADAKGNQFYLDFDGVMSRPKIYVN
                     GHLAGEWKYGYSSFRVDITPFLKFGQQNTIAVRVDNPPSTSRWYPGGGIYRHVWLTES
                     NPVHIEHWGVFVKTPEITKSAAKVEVDTTVKNTTDKAVIPTVTEEILDGGKIVASTTT
                     KGEEIPAGEKGKITSTLTLKNPTLWTLNAPHLYKMKTTVRMGDKVIDQKFTNFGVRTV
                     EWKPTGFYLNGERVQLKGVCQHHDLGPLGSAAHTRGYERQIEILKEFGVNSIRTSHNP
                     PAPEVLDLCDKMGILVIDELFDVWQCSKEGVNNESFNEWHERDVVNLCHRDRNHPCVI
                     AWSSGNEVPEQGMKNLHHISQTLTDLFHREDPTRKVTSGCNNANAARNGFGDTLDVYG
                     YNYKPWAYKDFAKDRPHQPFYGAETASCVSSRGEYFFPVDWNKGKGFYLYQVSSYDLY
                     APGWANRPDVEFAAQEDNPNSAGEYV"
     assembly_gap    3496..3513
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3514..4326
                     /locus_tag="PK077_01265"
     CDS             <3514..4326
                     /locus_tag="PK077_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4982 domain-containing protein"
                     /protein_id="HRN22963.1"
                     /translation="EPTPYNLDATNALNVPEGPEREKLMAELKKLGDRAPSRSSYFGI
                     VDLCGFKKDRFYIYQAHWRPDLKMAHILPHWNWPERKGQVTPVHVYTSGDEAELFLNG
                     KSQGVRKKGTGEKDRYRLVWEDVKYTPGTLKVVAKKDGKIWATDTVTTTGKPAALTLK
                     PDRNEIKGDGYDLSYVTVAVRDAQGRMVPRSKNQLTFRVSGPADIAGICNGDPTDFTT
                     MANPENKKIMKIKAFNGLAQVILRSRKGESGKVTLQVISNGLKPAQTTVTVK"
     gene            4481..7153
                     /locus_tag="PK077_01270"
     CDS             4481..7153
                     /locus_tag="PK077_01270"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRN22964.1"
                     /translation="MSDLPKAYDPSLVEEKWQSRWIEEGCFKADPASEKPAYSVVIPP
                     PNVTGVLHLGHVLNNTIQDILVRRARQKGYEALWLPGTDHAGIATQVRVEKDLKQTTG
                     RSRHDLGREAFLEKVWEWKEKHGGIIINQLHKLGCSCDWDRERFTMDEEYTKAVGQVF
                     IELFKEGLIYRGRRMVNWCPVSLTALSDEEVIMTEQKSKLYTVLYKLEDGSGALHVAT
                     TRPETIMADVAVAVNPKDPRYAHLIGKNVMRPLNPTPIPIIGDEYVEIEFGTGALKIT
                     PAHDKADFEIGRKFNLEIIDILTPDGHINCPEVPELHGMDRFDARRKSVEMLEASGLM
                     VNIEDYDNKVGFSERANVPIEPRLSMQWFLKYPCVKEAADAVAGGDITFRPARWAKTY
                     AHWLENIQDWCISRQLWWGHRIPVWYRKDKAEELRNAPALDASALEQGFLYVGTEPPQ
                     DPDNWIQDNDVMDTWFSSWLWPFSTMDEETRAKFYPTTDLVTGPDIIFFWVARMIMAG
                     YRFQHDKPFSNVFFTSIIRDKIGRKMSKSLGNSPDPLDLIANYGADGLRFGLMRIAPT
                     GTDVRFDENQISEGRNFANKLYNATRFRLMQGDARGETAPHYSSVHISIISKLKQLHA
                     DVEKALADYEFNALIQTLYQFFWNEYCDRFLEAVKGDLKDGADPAARAATLTTMDTVL
                     RHYLALLHPVMPHITEELWASLGFAESNGGLPLMRTPLPSAENLLAGLDESRITLANT
                     QAAALYETANKARNLKAEYDLSNNKNVSFILKTPHDVPQDILSRLAILANAKSVIRDA
                     AYSSPKGTPAALTPLGELFLPLEGLIDVEAEKERLGRELDKIAREIAKSAAKLGNAGF
                     VERAPAEVVNQEKARLADWEAKQSQLKGMLDSLS"
     gene            7172..8014
                     /locus_tag="PK077_01275"
     CDS             7172..8014
                     /locus_tag="PK077_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRN22965.1"
                     /translation="MNNIQKYFAGREQFIELLEAVGIRTLEQFASADPSTVLPELHQA
                     KRMLKLQTEIPSAPVFREWVNQALSSPTAPEPELLPIHEGDGLPLATPVDPSITDSSE
                     NRRERGRSHTDGPPKHLSAKARLQEEHSYQPAKHVHTHKEPKTYLRKKGIKHMTSFRT
                     WMGAVIVLLLFICILFSISVTTLVLLNGERGWPLISLCFGPWILALVLYLSLALPRKC
                     SVCRAHVFSFKKYTRNKAAHHIPLFGYVFATALHIFLFRWFRCPACGSSQQLGKPRTE
                     PHRH"
     gene            complement(8626..8991)
                     /gene="rplN"
                     /locus_tag="PK077_01280"
     CDS             complement(8626..8991)
                     /gene="rplN"
                     /locus_tag="PK077_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRN22966.1"
                     /translation="MIQMESLVQVADNTGARSAKMIGVIGKRTRQAHIGDIITCHIRE
                     SIPTASVKKGTVVKAVVVRTAAPIRRDDGSVLRFDGNAVVIIDKDNNPRGTRIFGPVA
                     RELREKKFMKIVSLAPEVL"
     gene            complement(9029..9301)
                     /gene="rpsQ"
                     /locus_tag="PK077_01285"
     CDS             complement(9029..9301)
                     /gene="rpsQ"
                     /locus_tag="PK077_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRN22967.1"
                     /translation="MSEQTETTKKPGLRKTRVGVVTSTKMDKTIVVEYVARVPHPKFK
                     KIVKKSKKFYAHDENSTAKVGDKVRIVETRPLSKLKCWELVEVLTH"
     gene            complement(9350..9553)
                     /gene="rpmC"
                     /locus_tag="PK077_01290"
     CDS             complement(9350..9553)
                     /gene="rpmC"
                     /locus_tag="PK077_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRN22968.1"
                     /translation="MSDKNSAKELRAMSAKELSALVRSLREELFNLRLQQATGQLENN
                     QRIRTVRKDLARALTIQGETGEV"
     gene            complement(9568..9990)
                     /gene="rplP"
                     /locus_tag="PK077_01295"
     CDS             complement(9568..9990)
                     /gene="rplP"
                     /locus_tag="PK077_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRN22969.1"
                     /translation="MPLMPKRVKHRKMHRGSRSGNATSGTYVAFGDFGLQVLDRGWIT
                     NNQIEACRIAINRYLKRKGKVFIRIFPQKSFTSRPPDTRMGKGKGAVEGWVAVVRPGN
                     ILFEVGGVTESQAREALRLASNKLGVSTRFVYRQGVQH"
     gene            complement(10043..10732)
                     /gene="rpsC"
                     /locus_tag="PK077_01300"
     CDS             complement(10043..10732)
                     /gene="rpsC"
                     /locus_tag="PK077_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRN22970.1"
                     /translation="MGQKVNPIGFRLAVNKDWRSKWYATGQDYATKLHEDLVMRKYIK
                     EQLMSAAVSSIVIERAWNSVRITVHTARPGLVIGRKGEEIEKIRQYLQSLCGASTQVN
                     IDIVEIRSPETDAQLIAENVAVQLERRVSFRRAMKRAVQVAMERGADGIRIRCAGRLG
                     GADIARAEWYREGKVPLQTLRTPIDYGFAEARTLYGIIGVKCWVNKRDEVVSQQNSRP
                     SGPRGPRRPRA"
     gene            complement(10772..11110)
                     /gene="rplV"
                     /locus_tag="PK077_01305"
     CDS             complement(10772..11110)
                     /gene="rplV"
                     /locus_tag="PK077_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRN22971.1"
                     /translation="MEVKAVYKYARISAKKMRDVAQEIQGLSVSQATDILSYTPKKGA
                     YLLNKTLKSALANAENNAELSVDTLVVKSVMVDEGPTMRRTMPRARGSANMIRKRTSH
                     ITVILADQEG"
     gene            complement(11149..11859)
                     /locus_tag="PK077_01310"
     CDS             complement(11149..11859)
                     /locus_tag="PK077_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22972.1"
                     /translation="MSNSSSILAYSLTTLVGLGGALQGAEEDTAALTRRIERLEKQNA
                     ALKASYVQARSDADKAAAQLVDIRSRLEALGGAALGNSEERLIQAMADIEAFNDRVQR
                     LEQASVKLSGAILAYMKQAISEDANARTAVESSLRLLESVLGYRQQPVRDGAGTLAEA
                     KVLSIDSESGVVVLNTGRTAGMQVGMPVRLTRGAQTIGEAVVTDVRKEVCGALVQKLE
                     TPADPVRVGDSASVKTND"
     gene            complement(11880..12149)
                     /gene="rpsS"
                     /locus_tag="PK077_01315"
     CDS             complement(11880..12149)
                     /gene="rpsS"
                     /locus_tag="PK077_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRN22973.1"
                     /translation="MGRSLKKGPFVSQKLLAKIDAQLESGDRKPIKTWSRASMITPDF
                     VGLTFLVHAGKNFATVYVTENMVGHKLGEFAPTRVFKQHGGIGKK"
     gene            complement(12185..13021)
                     /gene="rplB"
                     /locus_tag="PK077_01320"
     CDS             complement(12185..13021)
                     /gene="rplB"
                     /locus_tag="PK077_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRN22974.1"
                     /translation="MSLKSFKPVTPSNRYKVWPSFDEITTSTPEKSLCRPLKKSGGRN
                     NNGRITTRHIGGGHKRKYRLVDFKRNKFDVPATVLTIEYDPNRTCRIALIEYKDGEKS
                     YILAPTGLQVGMKVESGQKVAPKVGNAMPLKNVPLGTSVHNIEIRPGSGGKVARAAGQ
                     QAIVSNREAGYALIKMPSGEIRRFNEDCYCTIGQVGNTQHMNEMSGKAGRTRWMGVRP
                     TVRGMTMNPVDHPNGGGEGKSKSGGGRQHLKSPWGHVKGQKTRRLRKPSDSVIVQRRN
                     AK"
     gene            complement(13048..13332)
                     /gene="rplW"
                     /locus_tag="PK077_01325"
     CDS             complement(13048..13332)
                     /gene="rplW"
                     /locus_tag="PK077_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRN22975.1"
                     /translation="MKDIYQVIKKVRISEKATMLQETTGELVFEVDRDANKIEIKKAV
                     EVAFGKKVASVRTANYDGKLKRQRRSDAGRTAHWKKAYVKLANGETLDLV"
     gene            complement(13339..13947)
                     /gene="rplD"
                     /locus_tag="PK077_01330"
     CDS             complement(13339..13947)
                     /gene="rplD"
                     /locus_tag="PK077_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRN22976.1"
                     /translation="MSANTFTLEAAAAANIQVVGSDKGSQAVHDLIVAYQANRRTGSA
                     NSKTRAEVSGNNKKIFRQKGTGNARHGDKRAPIFVGGGVVFGPRPCDYSKKVNKSTRR
                     LALRRVLGDLIAASKVSTVSEFSVADGKTKSFIKAVKDLTDAKKVLIVAASFDETTYR
                     AARNVQEVLLMTAAEVNIEQLMNADAVILVDNALETLASRTA"
     gene            complement(13989..14624)
                     /gene="rplC"
                     /locus_tag="PK077_01335"
     CDS             complement(13989..14624)
                     /gene="rplC"
                     /locus_tag="PK077_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRN22977.1"
                     /translation="MALGLIGKKVGMTRLFDQESGAMVPVTVIDVKGNTFAQIKTEEK
                     DGYNAIQVAFDAQKESRVAKPQAGHFKKLGIQPTKLLKEFRVEASELPAEGAEDPGVD
                     LFSAGQWVDVIGTSKGKGFQGAMRRHNFHGSPAAHGSMMHRRTGGVGGCSTPGRVWKN
                     QKMPGQMGNAKRTVQNLKVVAVRPEDNVILISGAVPGARGSYLVIRPAKKK"
     gene            complement(14670..14978)
                     /gene="rpsJ"
                     /locus_tag="PK077_01340"
     CDS             complement(14670..14978)
                     /gene="rpsJ"
                     /locus_tag="PK077_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRN22978.1"
                     /translation="MQSPKIRIRLRAFDYRAIDRSSQEIVETAKRTGAKVHGPIPLPT
                     RIEKFSVNRSVHVNKKSAEQFEIRTHKRLLDIVDPTARTIDELKKLNLPAGVDITIRI
                     "
     gene            complement(15016..17163)
                     /gene="fusA"
                     /locus_tag="PK077_01345"
     CDS             complement(15016..17163)
                     /gene="fusA"
                     /locus_tag="PK077_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRN22979.1"
                     /translation="MSDHVNNPNRKAPLERYRNIGISAHIDAGKTTLSERILFYTGMI
                     HKIGETHDGSTTTDWMEQERERGITITSAAVTTNWKQVKNEGISKVFEGENFQLNIID
                     TPGHVDFTAEVERSLRVLDGAIVVFCGVAGVQPQTETVWRQATKYSVPRMCFVNKMDR
                     TGANFNNVLDDIHNKLGANAAAILIPIGAEDQLRGQIDVVNQKAVIYADNDRLGSTYT
                     IEDIPAELKEEAELAYHELVSRVADVDDELAEKVLMEEPFTPKELKEAIRRATIANKF
                     VPVAGGSAFKNKGVQFLLDAVVDYLPSPVETVPAHAESTLDPEKTFEITATDDAKPMV
                     LAFKLWADKFVGKLVFIRVYSGVLKKGDTVYNPRTRKTERVGRIIQIQADQHTDIDAV
                     YSGDIAAIVGLRNVTTGDTITSPDNDICLEPPTFPETVISMAVEPKTKADQEKMSNAL
                     GRLSEEDPTFRVKTDEETGQTLISGMGELHLEIIIDRLMREFKVEADIGKPQIAYRET
                     ITAPAHGDGKLVKQSGGRGQYGHVVIDVKPNERGKGLTIENKIVGGAIPKEYMNAVYA
                     GLNEAMTTGVVAGYPVVDVHVEVVDGSYHEVDSNENAFKMAAIFAMKDAFKKAKPIML
                     EPIMSVEASTPTDYQGDIMGDLNRRRGQISNMENKANACILKAMVPLSEMFGYSTAIR
                     TLSSGRASYSMEPSHFEQVPQNLVDQIVSEKAK"
     gene            complement(17227..>17633)
                     /gene="rpsG"
                     /locus_tag="PK077_01350"
     CDS             complement(17227..>17633)
                     /gene="rpsG"
                     /locus_tag="PK077_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRN22980.1"
                     /translation="GKLISKVMLDGKRSLAERIVYAAIDMANEGTDSIDPLEVITRAI
                     ENAKPRVEVKSRRVGGATYQVPLEVDPARSESLAMRWIVNYARNRKGVPMHKALANEI
                     KEAANNQGSSVRKRDDVHKMAQANRAFAHFRW"
BASE COUNT         4001 a         5048 c         4765 g         3801 t
ORIGIN      
        1 tgcccctgtc cgcacagggg caaagcatcg tggattcacg ggcctccctt ccacgggccg
       61 tccagcttca cggggatgaa ggcagggaaa cggtgcggcc agacgaatcc aagcgcatct
      121 ctccgaacga agcgccggca tcccgtgttc agccccagcg gatcgtcaac cggattgccg
      181 ccacagtcaa cggccgtccc atcacggcca atgaggtcag cgtgcgtctc atgcccatcg
      241 gagcacagct cgcggcccag tatcccaaac aggggcccga attctacaag caactggccc
      301 tggcgaaaaa aaacatcatt gaagacctgg tggagcgaga actgctccgc aatgaatttg
      361 aaggcatggg cggcgtcatt agggactctc tcattgacca ggaagtcaac cgcaccatcc
      421 tgaccacctt taacggagat cgttccgcct tcctgaaaaa cctgagcctc tccggcatga
      481 ccatccgcgc tttccgggaa atgacgaaaa aacagcttca ggttcaaatc atgcgggctt
      541 ccaaatacga ccaggaaata ccgcctactc cggaggaaat acagcaggaa tacgaatcta
      601 ccaaagaaca ataccgggac ctgaccaaag acaaaatcaa attcaagaaa atcttcattc
      661 ccatgctggg ggatgactcc gcctccacgc cggaagtaca gctgaacctg gcggaactca
      721 tcgccaagga aatcaaatcc aaaaacgcca cctttgagga aatggccaaa cgctactcca
      781 aggatctata tgcggaaaaa ggcggtgact ggcccgttac ggaacgctcc acgctctccc
      841 cggaatccgc cgccatcatc tttggcgcac agcccggcga aatcatcgga ccgttggtgg
      901 actccaccgg cttcaccatc gtattggtgg agaaaaagga actggcgccg ccgccccccc
      961 tctccgccat caaggagcag attgacatca tggcgcgcaa caagcgcagc aatgaacgtt
     1021 ataaaaaatg ggtggaacgc ctccggaaaa aagccatcgt caaaatatac atctgattct
     1081 tctcctttcc ccattgccgg accggcccat tcaacaaggc cggtccggcg ttctctcttt
     1141 catttcccct gtttttcttt ccgccaaccg ttccgtccgc tctccttctc ctgccggggg
     1201 catcttcctc tcgcccatat ctctggaaga cgccttcata cataactgga cggcggccgc
     1261 aagtcatccc gcctgacgcg tggaaccctg ctgacgtaat ccgggcagaa accgtatcct
     1321 gctgaataaa aatcttgcaa gatggcagat ttccagtcgt aatatcacag atatgttcac
     1381 caatattaca cgcacgaccc tctgcattac tgcgttcagc atcgccagcc tcatggcggc
     1441 gcctctgaat gcaaccaaaa cggagagcct ggattggaac tggaaattcg cccgtttcgg
     1501 gaaaatgccg gatggcagta cgcaaccgga accgggaaaa gccatgggat tcgccactgc
     1561 caccagtgaa gaatccggca atccggcgga caatgccgtg gacggggaca agtccacccg
     1621 ctggtgtgcc gccagtggca aaagcggaga aaaaatcacc gtggacatgg gacgccccgt
     1681 agatgtaaaa accgttaaca tcctgtggga aaaacaaagc aaccatcttt tcaagctgga
     1741 aggctccggt gacggaaaac gctgggcaac tattgaagac aaaacttccg ggcaaaacga
     1801 ctccaaggaa gacacggtag aaaacaaaac cggcaaaccg cgatacttcc gcatcaccgt
     1861 cacgggcaat aaccagagca actgggccag catccgtgaa atcaccttta aaaacgacaa
     1921 gggggaaatt atccgccctc aggccgccgc cggaaccagt aagtcggaca atccctccag
     1981 cccctctttc aacgacaaaa actggcgttc cttgaacctg ccgcacgact ggggcgtgga
     2041 aggccccttc cggatggaaa ttgaaaacag aaccggaaaa ctcccctggg tcggcattgg
     2101 ctggtaccgc aaaacgctgg aaatcccggc ggacgccaag ggcaaccaat tctatctgga
     2161 ctttgacggc gttatgtccc gccccaaaat ttatgtgaac ggacacctgg ccggcgaatg
     2221 gaaatacggt tacagctcct tccgcgtgga catcacgccc ttcctgaaat tcgggcagca
     2281 aaataccatt gccgtcagag tggacaatcc ccccagcacc tcccgctggt atccgggcgg
     2341 cggcatctac cgccatgtgt ggctcacgga atccaaccct gtgcacatcg aacactgggg
     2401 cgttttcgtc aaaactccgg aaatcaccaa atccgccgcc aaggtggaag tggacaccac
     2461 ggtaaaaaac accacggaca aagccgtcat ccccactgtt actgaagaaa tcctggacgg
     2521 aggtaaaatc gtagcctcca caaccaccaa aggggaagaa attcccgccg gggaaaaggg
     2581 caaaatcacc agcacgctga cgctcaaaaa ccccactctg tggaccctaa acgctcccca
     2641 tctgtataag atgaaaacca cggtcaggat gggagacaaa gtcatcgacc aaaaattcac
     2701 caacttcggc gtaagaaccg ttgaatggaa acccacggga ttctacctta acggggagcg
     2761 cgtgcagctc aagggcgttt gccagcacca tgacctggga ccgctcggct ccgccgccca
     2821 cactcgaggc tatgagcgcc agattgaaat cctgaaggaa ttcggcgtca actccatccg
     2881 cacgtcccac aacccgcctg cgccggaagt gctggacctg tgcgataaaa tgggcatcct
     2941 agtcattgac gaacttttcg acgtatggca atgctccaaa gaaggcgtca acaacgaatc
     3001 ctttaacgaa tggcatgaac gggacgtggt taacctctgc caccgggacc gcaaccaccc
     3061 ctgtgtcatt gcatggagtt cgggaaatga agttccggaa cagggaatga aaaatctgca
     3121 ccatatctcc caaaccctga cggatctttt ccaccgggaa gaccccacgc gcaaagtgac
     3181 ttccggctgc aacaacgcca atgccgcacg caacggcttt ggggacaccc tggatgttta
     3241 cggctacaac tacaagccct gggcctacaa ggacttcgcc aaggaccgcc cccaccagcc
     3301 gttctatggt gcggaaaccg cctcctgcgt cagctcccgc ggagaatact tcttccccgt
     3361 ggactggaac aagggcaagg gattctacct ctaccaggtc agttcctatg acctgtacgc
     3421 ccccggctgg gccaaccgtc cggatgtgga attcgccgct caggaagaca atcccaacag
     3481 cgcgggagaa tatgtnnnnn nnnnnnnnnn nnngaaccca ccccgtacaa tctggacgcc
     3541 accaacgccc tgaacgtgcc ggaagggccg gaacgcgaaa agctgatggc ggaactcaaa
     3601 aaactgggag accgcgcccc ctcccgcagc tcctacttcg gcatcgtgga cctgtgcggc
     3661 ttcaaaaagg accgcttcta catctaccaa gcccactgga ggccggatct caagatggcg
     3721 cacatcctgc cgcactggaa ctggccggaa cgcaaggggc aggtaacacc cgtgcatgtc
     3781 tacaccagcg gggatgaagc ggaactcttc ctgaatggga aatcccaggg cgtccgcaaa
     3841 aagggcaccg gggaaaagga ccgctaccgc ctcgtgtggg aagacgtcaa atacacgccc
     3901 ggcaccctca aagtagtcgc caaaaaggac ggtaaaatct gggctacgga cacggtaacc
     3961 actaccggaa aacctgcggc actcaccctc aagccggacc gcaatgaaat caagggagac
     4021 ggctatgacc tgtcttatgt caccgtagcc gtccgcgacg cccagggccg tatggtgccc
     4081 cgaagcaaaa accagctcac cttcagggta agcggccctg cggacatcgc cggcatctgc
     4141 aatggtgatc ccacggactt caccaccatg gcgaatccgg aaaacaagaa aattatgaaa
     4201 atcaaggcct tcaatggtct tgcccaggtc attctgcgct cccgcaaggg agaatccgga
     4261 aaagtgacgc tccaagtcat ctccaacgga ctcaagccgg ctcagacaac tgtgacggtc
     4321 aaataaaaaa acaggaaaaa ctccgttcca gccgccgggc tccatcagga aacccggcgg
     4381 ctttttcatc acattattcc ggggaaaaag agccatcttt ccaccctctc ccggacaggt
     4441 agggattgaa agaaaaaaac tttatgctac ccttttgccc atgtctgatc tgcccaaagc
     4501 atacgatccc tccctggtgg aagaaaaatg gcaatcccgc tggattgaag aaggttgttt
     4561 caaggctgac ccggcttctg aaaagcctgc ctactctgtc gtcatccctc cccccaacgt
     4621 cacgggagtg ctgcatctgg gccatgtgct gaacaacacc attcaggaca tcctggtgcg
     4681 ccgcgcgcgc caaaaaggat acgaagccct ctggctgccc ggaacagacc atgccggaat
     4741 cgccacgcag gtaagggtgg aaaaagacct caaacagaca acgggccgga gccgccacga
     4801 tctgggccgt gaagccttcc tggaaaaagt ctgggaatgg aaagaaaaac acggaggcat
     4861 catcatcaac cagctccata agctgggttg ttcctgcgac tgggaccgcg aacgcttcac
     4921 gatggatgag gaatacacca aagccgttgg acaggtcttc attgaactct ttaaggaagg
     4981 actcatctac cggggccgcc gcatggtcaa ctggtgccct gtatccctca cggccctctc
     5041 tgatgaagaa gtcatcatga ctgagcagaa aagcaagctc tacaccgttc tttacaaact
     5101 ggaagacggg tccggagccc tccatgtagc caccacccgc ccggaaacca tcatggcgga
     5161 cgtggctgtg gccgtcaacc cgaaggatcc gcggtacgcc cacctcatcg ggaaaaacgt
     5221 catgcgcccg ctcaatccca cccccattcc tatcatcgga gatgaatatg tagaaataga
     5281 attcggcacg ggcgctctga aaatcactcc ggcccatgat aaggccgact ttgaaatagg
     5341 ccggaaattc aacctggaaa ttatcgacat ccttaccccc gacggtcata tcaactgccc
     5401 ggaagtgccc gaactccacg gcatggaccg ctttgacgca cgccgcaaat ccgtggaaat
     5461 gctggaagcc tccggcctca tggtcaacat tgaagactat gataacaaag tcgggttctc
     5521 cgaacgcgcc aatgtcccga ttgaaccgcg cctctccatg cagtggttcc tcaaataccc
     5581 ctgcgtgaaa gaagccgcgg acgccgtagc gggaggggat atcacttttc gccccgcgcg
     5641 ctgggcgaaa acctacgccc actggctgga aaacattcag gactggtgca tctcccgaca
     5701 gctctggtgg ggccatcgca tcccggtctg gtaccgcaaa gacaaggcgg aagaactcag
     5761 gaacgccccg gcgctggacg cctccgcact ggaacagggc ttcctctacg tgggaaccga
     5821 accgccccag gacccggaca actggattca ggacaacgac gtaatggaca catggttctc
     5881 ctcctggctg tggcctttct ccaccatgga tgaggaaacc cgcgccaaat tctatcccac
     5941 tacggatctt gtcacgggac cggacatcat cttcttctgg gtggcgcgca tgattatggc
     6001 cggataccgt ttccagcatg acaaaccgtt cagcaacgtc tttttcacgt ctatcatccg
     6061 tgacaaaatc gggcgtaaaa tgagcaaatc cctgggcaac tccccggatc ctctggacct
     6121 gatcgccaac tacggagcgg acggcctccg cttcggactc atgcgcatcg cccccacagg
     6181 aacggacgtg cgctttgatg aaaaccagat cagcgaaggc cgcaactttg ccaacaagct
     6241 gtacaatgcc acgcgcttcc ggctgatgca gggagatgcc aggggagaaa cggcccctca
     6301 ctactcatcc gtacatatct ccattatctc caagctcaag caactgcatg cagatgtaga
     6361 aaaagcgctg gcggactacg aattcaacgc cctcatccaa accctgtacc aattcttctg
     6421 gaacgaatac tgcgaccgct tcctggaagc cgtcaagggt gacctgaagg acggggcgga
     6481 cccagcggca cgggcagcca cgctcaccac gatggacacg gtgctcaggc actacctggc
     6541 ccttctgcat ccggtcatgc ctcatatcac ggaagaactc tgggcctccc tgggctttgc
     6601 ggaaagcaac ggcggcctgc cgctcatgcg cactcctctc ccctctgccg aaaacctcct
     6661 ggcaggactt gatgaaagcc gcatcacctt ggcgaatacc caggccgcag ccctttatga
     6721 aacggccaac aaggcccgca acctgaaagc ggaatacgac ctttccaaca ataaaaacgt
     6781 cagcttcatc ctgaaaactc cccacgacgt accccaggat atcctctccc gtctggccat
     6841 tttggcgaac gccaaatccg tcatccggga cgcagcttac tcctccccca agggaacgcc
     6901 tgcggccctc acgccgcttg gggaactctt cctgcccctg gaaggcctta ttgacgtgga
     6961 agcggaaaag gaacgcctgg gcagggaact ggacaaaata gctagggaaa tcgccaaatc
     7021 cgcggccaag ctgggtaatg cgggctttgt ggaaagagct ccggcagaag tcgtgaatca
     7081 ggaaaaagcc cgtctggcgg actgggaggc aaaacaatcc cagttaaaag ggatgcttga
     7141 ttccctttcc taaccatcta tcattccggg aatgaataat attcaaaagt actttgccgg
     7201 ccgcgagcaa ttcattgaat tgcttgaagc ggtgggcata cgtactcttg aacaattcgc
     7261 ttccgcggac ccctccaccg ttttgcctga acttcaccag gcaaaacgga tgctgaaact
     7321 gcagacggaa attccttccg ctcccgtttt cagggaatgg gtgaatcagg ctctttcttc
     7381 tcctactgcg ccggaaccgg agcttcttcc catccatgag ggcgacggcc tcccccttgc
     7441 cactcccgtt gacccttcga ttactgattc ttcagaaaac agaagggaac gtgggcgttc
     7501 ccatacggac ggacctccaa aacatctctc cgccaaggcc aggctgcagg aagaacacag
     7561 ctaccagccc gcaaaacatg ttcacaccca caaggagccg aaaacctacc ttcgcaaaaa
     7621 aggcatcaag cacatgacct ccttccggac atggatggga gccgttatcg tcctgctcct
     7681 gttcatctgc atcctcttct ccatttcggt caccaccctt gtgcttctca atggagaacg
     7741 aggctggccg ttaatcagcc tctgcttcgg cccctggatt ctggcgctgg ttctctatct
     7801 ttccctggcg cttccccgga aatgcagcgt gtgcagggcc catgtctttt cctttaaaaa
     7861 atacacccgc aataaggcgg cccaccatat tcccctgttc ggctacgtat tcgccacggc
     7921 gctgcatata ttcctcttcc gctggttccg ttgcccggcc tgcggttcct cccagcaact
     7981 gggaaaaccc cgtaccgaac cgcacaggca ttaacgaagc ttaattcctg cgccaggctt
     8041 cacggtatcc cggcctcctg cctcctttct tccccttacc tggcattcgg aaggcggttc
     8101 ctccatctgt ccgtcccttc cattatctgc ccctattccc cgcacttcaa ccccacgctc
     8161 tgacgggaca ggaagcccct gcccgcttcc gtatctcatg gaacggctcc cggcaacgcc
     8221 cccttccggt aacttattct ataaaaagcc accatgagga accgcctcaa aaccggctcg
     8281 gaaaaatccg gcaagaaggc cgtttctccg tctcttccgg ataaatacgt ttcctccttc
     8341 ctccggatat cctagcaact caaacatgcg ttcaacgtca tggaaaagca tatccctgaa
     8401 aacggcagtc cagaaaaaca accggacatt tcaacaaccg gaattccccc ctcataacct
     8461 attccggatt tgccggcctc ctgcgccagg caagggctgc gctccggcaa ccttctccct
     8521 atatgggaag cacaccgtaa tgaaaactta tcagaaaaac ggggacaata aaaaacgcca
     8581 tcctcctttt cggaggatgg cgtccaaaat cctgagacgt ctcggttaga gcacttccgg
     8641 agcaagggaa acaatcttca tgaacttctt ttcacggagt tcacgagcga ccggcccgaa
     8701 aatacgggtg ccgcgcgggt tgttgtcctt gtcgatgatg acgacggcat tgccgtcaaa
     8761 acggagcacg gaaccgtcat cacggcgaat cggggcggcg gtgcgcacta ccacggcctt
     8821 caccacggtt cctttcttca cggaagcggt gggaatggat tcgcggatgt ggcaggtaat
     8881 aatgtcgcca atgtgagcct gacgggtgcg cttgccgata acgccaatca tcttggcgga
     8941 gcgggcgccg gtattgtcgg ctacctggac tagggattcc atctggatca tggtatcaaa
     9001 tctcctttac ttaatggttg attcctgatt aatgggtaag cacttccacc agttcccagc
     9061 acttcagctt ggaaagcggg cgggtttcaa cgatacgtac cttatcgcct accttggccg
     9121 tggaattttc gtcatgggca tagaacttct tgctcttctt gacgatcttc ttgaacttgg
     9181 ggtgcggaac gcgggcaacg tattccacaa cgatggtctt gtccatcttg gtggaagtca
     9241 ccacgccgac gcgcgtcttg cgaaggccgg gcttcttggt tgtttcggtt tgttcggaca
     9301 ttggattcta aaagtgttag aatattctgt aattaccgga aatcctcaat taaacctccc
     9361 cggtttcacc ctgaatggta agggcgcggg caaggtcctt gcggacggtg cggattctct
     9421 ggttgttttc caactggccg gtggcctgct ggaggcggag attgaaaagt tcttcacgaa
     9481 ggctgcgcac cagagcggaa agctccttgg cggacatagc acgaagttct ttggcggagt
     9541 tcttatcgga catggtgatg agtagcttta gtgttgaacg ccttgacgat agacgaagcg
     9601 ggttgatacg cccagcttgt tggaagccag tcgaagggct tcacgcgcct gggattcggt
     9661 cacgccgcct acttcaaaca gaatgttgcc ggggcgaacc acggctaccc agccttccac
     9721 ggcgccctta cccttaccca tacgagtatc cgggggacgg gacgtaaagg atttctgcgg
     9781 gaaaatgcgg atgaacacct tacctttacg tttcaggtaa cggttgatcg caatacggca
     9841 ggcttcaatc tggttgttgg tgatccaacc gcggtcgagc acctgaaggc cgaagtcacc
     9901 aaaagcaaca taggtaccgc tggtggcatt gccggaacgg ctgccgcggt gcatcttgcg
     9961 gtgcttcact ctcttgggca ttaaaggcat atcttattcc tccttgtgtt aagtgtgatg
    10021 aacaaatgtg gtagcggctt tcttaggcgc gcggacgacg gggtccgcga gggccggacg
    10081 gacgggaatt ctgctgggag acgacttcat cgcgcttgtt gacccagcac ttcacaccga
    10141 tgatgccgta cagggtacgg gcttcggcaa agccgtagtc aatcggagtg cggagcgttt
    10201 gcaacggcac cttgccttcg cggtaccatt cggcacgggc aatgtcagcc ccgccaagac
    10261 ggccggcgca gcggatgcgg atgccgtcgg ccccgcgttc catggcaacc tgcacggcgc
    10321 gcttcatggc gcggcggaag gaaacgcggc gttccagctg gacggccacg ttttctgcaa
    10381 taagctgggc gtccgtttca ggggaacgga tttccacaat gtcaatgttg acctgggtgg
    10441 aagcgccgca caggctctgg aggtactggc ggatcttttc aatttcctca cccttgcggc
    10501 caatgacaag ccccgggcgg gcagtgtgga cggtgatgcg gacactgttc caggcacgct
    10561 caatcacgat gctggaaacg gcggcggaca tcaactgttc cttgatgtac ttgcgcatca
    10621 ccaagtcttc atgcagcttg gtggcgtaat cctggcccgt ggcgtaccac ttggagcgcc
    10681 agtccttgtt gacggcgagg cggaacccga ttggatttac tttctgaccc atgatattag
    10741 ttcagctata tgtgtgttag attggtatgg cctagccttc ttgatcggcc aggataacgg
    10801 tgatatggga tgtacgcttg cggatcatgt tggcggatcc gcgggcgcgg ggcatcgtgc
    10861 ggcgcatcgt gggcccttca tcgaccatga cggacttgac caccagcgta tcaacggaca
    10921 gttccgcatt attctcggcg ttggccaacg cggacttgag cgtcttattc agcaggtagg
    10981 cacctttctt gggggtgtag gacaggatgt cggtcgcctg ggagacggac aagccttgga
    11041 tttcttgagc aacatcgcgc atcttcttgg cggagatgcg ggcgtatttg taaacagctt
    11101 tcacttccat gggtttttcc tcgtagtgca ggttattatg aagatgattt agtcgttggt
    11161 ttttactgag gcagaatctc cgacgcgcac cggatcggct ggcgtctcaa gtttctgaac
    11221 aagcgcgcca catacttcct tgcggacgtc cgtgactacg gcttctccga tggtttgagc
    11281 tccccgggtt aaccgaaccg gcatcccgac ttgcattcct gccgttctgc cggtattcag
    11341 caccaccact ccggattcgg aatcaatgct gagcaccttg gcttccgcca gggttcccgc
    11401 gccatctcgc acgggctgtt gcctgtatcc cagaacggat tccagcaggc gcagagaact
    11461 ctccacagcc gtcctggcgt tcgcatcttc ggatatggct tgtttcatgt aggcaaggat
    11521 ggctccggag agcttgacgg atgcctgctc caaccgctgg acgcggtcat taaaagcttc
    11581 gatgtccgcc atcgcctgaa tcaggcgttc ctcgctgtta cctagcgcag cgccccccaa
    11641 agcttccagc ctggaacgga tgtccaccag ctgtgcggcc gccttgtcgg cgtcgctgcg
    11701 ggcctggaca taggatgcct tgagggctgc attctgcttt tcgaggcgct cgattctgcg
    11761 cgtcagtgcc gccgtatcct cttccgctcc ctgcaacgcc ccgcccagcc ctaccagggt
    11821 ggtaagagag tatgcaagga tggaagaaga attcgacatg ttgattgtta acttactgat
    11881 tacttcttac cgataccgcc gtgctgtttg aagacacgag tcggggcaaa ttcaccaagc
    11941 ttgtggccta ccatgttttc cgtgacgtac acggtggcaa agttcttgcc agcgtgcacc
    12001 aggaaagtca ggccgacgaa gtcaggcgta atcatggatg cgcggctcca ggtcttgatc
    12061 ggcttgcggt cgccggattc aagctgagcg tcgatcttgg cgagaagctt ttggctgaca
    12121 aaagggcctt ttttgagaga acgtcccatg ttatataatc ctagttaaag ttataatata
    12181 ttgattactt ggcattgcgg cgctgtacga tgacggagtc gctgggcttg cggagacggc
    12241 gggtcttctg gcccttgacg tggccccacg gagatttgag gtgctggcga ccgccaccgg
    12301 acttggactt gccttcaccg ccaccgttcg ggtggtcgac ggggttcatc gtcataccgc
    12361 gcacggtagg acgaacgccc atccagcggg tacggccggc cttgccggac atttcgttca
    12421 tgtgctgggt attgcctacc tggccgatcg tgcagtagca atcttcattg aaacggcgga
    12481 tttcaccgga aggcatcttg atcagcgcgt aacccgcttc acggttggaa acgatggcct
    12541 gctggccggc agcgcgggct accttgccgc cggaacccgg acgaatttca atgttgtgaa
    12601 cggaagtacc cagaggaacg ttcttcaagg gcatggcatt gcctaccttg ggagcaacct
    12661 tctggccgct ttccaccttc atgcccacct gcagccccgt gggggccaga atgtaggatt
    12721 tttcaccatc cttgtactca atcagagcaa tgcggcaggt acggttggga tcgtattcga
    12781 tagtaaggac agtagcgggc acgtcaaact tgttgcgttt gaagtccacc aggcgatatt
    12841 tgcgcttatg gccaccgcca atgtggcggg tggtgatgcg gccattatta ttgcggccgc
    12901 cggatttctt gaggggtctg cagagactct tttccggggt agaggtggtg atttcgtcaa
    12961 aggacggcca caccttgtaa cggttagagg gagtaactgg cttgaatgac ttgagggaca
    13021 tgatagcttt ccttcctaat aggggcttta aacgaggtca agggtttcac cgttggcaag
    13081 cttgacgtaa gcttttttcc aatgagcggt gcgacctgca tcagagcggc gctggcgctt
    13141 gagcttgccg tcatagttgg cggtacgcac ggatgcgacc ttcttgccga aagcaacttc
    13201 gacagccttt ttgatttcga ttttgttggc gtcacgatca acttcgaaga ccagctcgcc
    13261 ggtagtttcc tggagcatgg tggccttttc gctgatgcgc accttcttga tgacttggta
    13321 aatgtctttc atggcttctt aagcagtacg gctggcgagg gtttctaaag cgttgtcaac
    13381 gaggatcact gcatcagcat tcatgagctg ttcaatgttc acttccgcag cggtcatcag
    13441 gagaacttcc tgaacgttgc gggcggcacg gtaggtggtt tcgtcaaagg aagctgccac
    13501 aatgagcacc ttcttggcat ccgtcaggtc tttgacggct ttgatgaaag atttggtctt
    13561 gccgtcagcc acggaaaatt cggaaacggt gctgaccttg gaagcggcaa tcaggtcacc
    13621 cagtacgcgg cgcagagcca ggcgtcgggt gctcttgttc accttcttgc tgtagtcaca
    13681 ggggcggggg ccgaagacca cgccgccgcc cacaaagatg ggagcgcgct tatcgccgtg
    13741 acgggcatta ccggtgccct tctggcggaa gatctttttg ttattgccgc tgacttctgc
    13801 gcgggtcttg gaattggcgg aaccggtgcg gcggttcgcc tggtaagcca cgatcaggtc
    13861 atgcacagcc tgggagccct tgtcactgcc tacaacctgg atgttggcgg cggcggcggc
    13921 ttctaatgta aaggtatttg cggacataga taggttttcc tataatcaat gtgaggttaa
    13981 ctaaagcctt acttcttctt ggcggggcga atcacgaggt aggaaccgcg cgcgccgggc
    14041 acagccccgg agataaggat gacattatct tccggacgaa cggcgacaac cttcaggttc
    14101 tgcaccgtgc gcttggcatt gcccatctgt ccgggcatct tctggttctt ccagacgcgg
    14161 ccgggggtgg aacaaccgcc tacgccgccg gtacggcggt gcatcatgga accgtgggcg
    14221 gcgggagagc cgtggaagtt gtggcggcgc atcgccccct ggaacccttt gcctttggaa
    14281 gtgccgatca catccaccca ctgtccggcg gaaaagagat ccacaccggg atcttctgcg
    14341 ccttcagcag gcaattcgga tgcttctacg cggaattcct tgaggagttt ggtaggctgg
    14401 atgcccagtt tcttgaagtg gccggcctga ggcttggcca cgcggctttc cttctgcgca
    14461 tcaaaagcga cctggatagc attgtagccg tccttctctt ccgttttaat ctgagcgaag
    14521 gtgttgccct tgacgtcaat gacggtcacg ggcaccatag cgccggattc ctggtcaaac
    14581 agacgggtca taccgacctt ttttccgata agtcctagag ccattgtagt gtgtctttct
    14641 gttttttaat tacttatccg ggtgtcaaac tagatgcgga tcgtgatgtc caccccggcc
    14701 ggaagattaa gcttcttgag ctcatcaatc gtgcgcgcag tcgggtcgac gatgtcgagc
    14761 aaacgcttgt gggtgcggat ttcaaactgt tcagccgatt ttttgttgac gtgaacggaa
    14821 cggttcacag aaaacttttc aatacgggtc ggcaacggaa tcgggccgtg aactttggct
    14881 ccggtgcgct tggcggtttc aacgatctcc tgggaggagc ggtcgatggc gcggtagtcg
    14941 aatgcgcgga gtcgaatgcg gatttttgga ctttgcatgg acgtgcggtc tattggttat
    15001 acttcaaagg tttgtttatt tagccttctc gctgacaatc tggtcaacga ggttctgggg
    15061 cacctgttca aagtgggagg gttccatgga ataggaagca cggccactgg aaagggttcg
    15121 gatggcggtg gaataaccga acatttcgga caaggggacc atagctttca aaatgcaggc
    15181 gttggccttg ttttccatgt tgctgatctg gccgcggcgg cggttcaggt cgcccatgat
    15241 gtcgccctga taatcggtcg gggtggaagc ttcaacggac atgatgggtt ccagcatgat
    15301 cggcttggct ttcttgaaag cgtccttcat ggcgaagatg gcagccatct tgaaagcgtt
    15361 ttcattggag tccacttcgt ggtaggaacc gtccaccact tcaacgtgca cgtccaccac
    15421 cggataaccg gcgacgacac ccgtggtcat ggcttcatta aggccggcat aaacggcgtt
    15481 catgtattcc ttgggaatgg cgccgcctac gatcttgttt tcaatggtaa gccccttgcc
    15541 gcgttcgttc ggcttcacat cgatgaccac gtggccgtac tggccgcggc cgccggactg
    15601 cttgaccagt ttgccgtcgc catgggcagg ggcagtaatg gtttcgcggt aggcaatctg
    15661 gggcttgccg atatcggctt ccaccttgaa ttcacgcatc aggcggtcaa taatgatttc
    15721 cagatgaagt tcacccatgc cggaaatcag ggtctggccg gtttcttcat cagtcttcac
    15781 ccggaaagtc gggtcttctt cagacagacg gcccagagca ttggacattt tttcctggtc
    15841 agccttggtc ttgggctcta ccgccatgga aataacggtt tccgggaagg tggggggttc
    15901 caggcagata tcattgtccg ggctggtgat ggtgtcgccc gtggtcacgt tgcgcagccc
    15961 cacgatggcg gcaatgtcac cggagtacac ggcatcaata tccgtatgct ggtcagcctg
    16021 aatctgaatg atgcggccca cgcgttccgt cttgcgggtg cggggattgt acacggtgtc
    16081 acctttcttg agaacaccgg agtacacgcg gatgaacacc agcttgccga cgaatttgtc
    16141 agcccagagc ttgaacgcca gcaccatggg cttggcatcg tccgtagcgg tgatttcgaa
    16201 ggtcttttcc ggatccagcg tgctttccgc gtgggcggga acggtttcta ccggagaggg
    16261 gaggtaatcc accacggcgt caagcaggaa ctggacgcct ttgttcttga aagcggagcc
    16321 accggcaaca ggcacaaatt tgttggcgat ggtggcgcgg cggatagctt ccttgagctc
    16381 tttgggcgtg aagggttctt ccatgagcac cttttcagca agctcatcgt ccacgtcagc
    16441 cacacggctc acgagttcat ggtaggccag ttcggcttct tccttgagtt cggcgggaat
    16501 atcctcaatc gtgtaggtgg aacccaagcg gtcgttgtcg gcgtaaataa cggccttctg
    16561 gttcaccacg tcaatctggc cgcggagctg gtcttcagcg ccgatgggga tcagaatggc
    16621 agcggcattc gcgcccagct tgttgtgaat atcgtccagc acgttgttga agttggcacc
    16681 ggtacggtcc atcttgttga cgaagcacat gcggggcacg ctgtacttgg tggcctggcg
    16741 ccacacggtt tccgtctggg gctgcacgcc tgccacgccg cagaacacca cgatggcgcc
    16801 gtcaagcacg cggagagaac gttccacttc agcggtgaag tcaacgtgcc cgggagtatc
    16861 aataatgttc agctggaaat tttcgccttc gaacactttg ctgatacctt cattcttcac
    16921 ctgcttccag ttggtggtaa cagcagcgga ggtaatggtg ataccgcgtt cgcgttcctg
    16981 ctccatccag tcggtcgtgg tggaaccgtc atgggtttca ccaatcttgt gaatcatgcc
    17041 ggtgtagaaa aggatacgct cggacaacgt ggttttgccg gcgtcaatgt gagcggaaat
    17101 accgatattg cggtaacgct caaggggagc cttgcgatta ggattattta cgtgatcgga
    17161 catagtgtct ggaaagtatc tgtagtgatc gaaagtgaat gattcggctg acagtcgcag
    17221 aaggaattac cagcggaaat gggcaaaggc gcggttggcc tgagccattt tatgaacgtc
    17281 gtcgcgcttg cgaacggaag aaccctgatt gttggcggct tccttgattt cgttggcgag
    17341 agccttgtgc atcggcacac ccttgcggtt gcgggcgtag ttcacaatcc agcgcatagc
    17401 cagggattcg gaacgggccg gatccacttc aagcggcacc tggtaagtag cgccaccgac
    17461 gcggcggctc ttcacttcca cacggggttt ggcattttca atggcgcggg tgattacttc
    17521 cagaggatca atgctgtccg tgccttcatt agccatgtca atagcagcat aaacaatacg
    17581 ttccgcaagg gagcgcttgc catccagcat aaccttgctg atcagtttgc caa
//
LOCUS       DAOMSU010000014        17560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_101_length_17560_cov_4.071580, whole genome shotgun sequence.
ACCESSION   DAOMSU010000014 DAOMSU010000000
VERSION     DAOMSU010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 17560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17560
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101_length_17560_cov_4.071580"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1575
                     /locus_tag="PK077_01355"
     CDS             <1..1575
                     /locus_tag="PK077_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22981.1"
                     /translation="AEKQADKADSPQAAVSPAAFCVRATVVKTVKKDSGKNGYAIIIS
                     EETASMPAWKAVADKLAEKPGGSIVTVKDSVFSKLDTLKKMAPRFMAVVARPEEIDRV
                     LVNDLHRLSRRLDDDPYGDCIWGIVTGYTPQAAMRIASATKPLVISRAMGTTNVDSSR
                     FKDSMSITDWQPFQYLEQHGSKGKVTPAFYTKGLKEQDKGDETTLGVTPKLMEYWKRY
                     SPQLFVTASHATQFNLEMPFGKGLIVSGNNRFYALDKKQFREFTTFLRGVLFNGKEDD
                     LLSFLNRIKAPVIETRPVPAVWVAAGNCLIGDAKKTKNSMAVTALSRYGFNQLVGYTV
                     PSWYGKGGWGTLGLLFSNHDASSLAEAWYLNNQFILDETMTRFPKLMDVHFNSPDING
                     IKNDPDFARGMNSAGYGMGKDQLGLIHDRDTVAFYGDPAWTARLDESRAPSPWHIDWN
                     DPADASKGFTVTANKDAKGRLGVWFPNRINAGKATVTIGGTATPIEKAGLLTNDFLLL
                     RELELKKGEKAVVEME"
     gene            1702..2235
                     /locus_tag="PK077_01360"
     CDS             1702..2235
                     /locus_tag="PK077_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN22982.1"
                     /translation="MTDYSIFFIIAVVATGVGALLFLLSLFGLGEHDLDFDADSGGAD
                     VGLLSIKSGIGFFLGFGWGGVLAQGLDWGMAASLAAAFFTGVLMFLIIGISMRFIMSL
                     KSDGTLNYETLKGMTGSVYISIPGNLQRGGQVTIAHPSQLLYLPAVQEGEHPLPSGTP
                     VEVVAVTAGIVTVKPLH"
     gene            2269..3771
                     /locus_tag="PK077_01365"
     CDS             2269..3771
                     /locus_tag="PK077_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRN22983.1"
                     /translation="MDKIIPIAILVLFIILTASWLFSRYRMCPPDKILIVFGKVGTGQ
                     PAKCYHGGSTFVLPVLQSYSYLDLNPINIDVPLQGALSSQNIRVDVPSSFIVGISTLP
                     EIMQNAAARLLGRSREEIRNLAAEIIMGQMRVVIASMTIEEINSDREKLIKGITEGVD
                     VELHKVGLHLINANITDIQDASGYINALGKEAAARAINDATIKVAEETRRGEIGKAEA
                     EKDQTIQVANARAIAIEGQNEAQIKIAESAAKLQVKQAEAKKLAEVAQKVQEAKTLEE
                     AYEAEKEAELKRAERERATQEANILVTARIEKSQREVQAQATAEVLKLEQEGKAQALL
                     IQRRAEAEAIRQLADGEAQATLLKKKAEGEGMEMVGRGEAAAIEAVLEGKARGFQQIV
                     QAAGSSEAASNLLVTEQLTKIVELQSGAIKGLKFDKVVVMGNGGNSSVGGFVQNLVTD
                     TLPLHELGKSVGLELPAFLGKPMEGKTAASSPAPAADAPAADTATTVNPS"
     gene            4186..4719
                     /locus_tag="PK077_01370"
     CDS             4186..4719
                     /locus_tag="PK077_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HRN22984.1"
                     /translation="MSVLLHICCAPCSISCIEMLREEGMEPTGFWFNPNIHPYKEYQA
                     RKDTLVEYATKIGLKLIMRDEYGLRPFIRAIYPSLENRCGTCYRIRFEETARFAAENG
                     VDGFSSTLFVSPYQNHELLRKTAEKTAEKFGTRFIYRDFRPGFREGQQKARELRLYMQ
                     KYCGCVFSEEERYLKKK"
     gene            4751..5683
                     /locus_tag="PK077_01375"
     CDS             4751..5683
                     /locus_tag="PK077_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRN22985.1"
                     /translation="MTLQQLKYIIGIVNSGSINEAAKRLFISQPSLSKAVRELENELN
                     FEIFRRSSKGISLSLEGTEFLSYARQVVEQAELLEQRYLNTKPLKRQFSISTQHYAFA
                     VNAFVNLIKKLNIQEYEWTLRETRTFEIIEDVHQSRSDLGILYLNDFNRQVISKLLKK
                     NDLCFHPLFRAEPHVFVSSAHPLARRRQVLLEDLEDYPCLSFEQGTYNSFYFSEEILS
                     TIVHRKEIHVSDRATLFNLLIGLNGYTICSGVLSEDLNGSNIISIPLKTKEYMLVGWI
                     DREQSRPSRSALLYIEELKRVITEYGYEVIRTDD"
     gene            complement(5680..6543)
                     /gene="metF"
                     /locus_tag="PK077_01380"
     CDS             complement(5680..6543)
                     /gene="metF"
                     /locus_tag="PK077_01380"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRN22986.1"
                     /translation="MKLSELFPQKRVLSFEVFPPKRTSPIGTVYHALSALQGLNPDFI
                     SVTYGASGGAGTSDTLNIASSIKKDYMVESVAHLPCISLTKGNVLELLEQFRRNGIEN
                     ILALRGDLPPDREPCRDFTYASDLISFIKEQGNFHILAACYPEGHPESKSIVDDIRHL
                     KTKVEAGADHLISQLFFGNDIFHAYREKLDLAGISVPLEAGIMPVVNKKQIERMVSLC
                     HVHLPKKFIRMMERFEHNPIAMRDAGIAYAVEQIIDLVAQGVDGIHLYTMNNPYVAGR
                     ICEATASIIRN"
     gene            complement(6540..8813)
                     /gene="metE"
                     /locus_tag="PK077_01385"
     CDS             complement(6540..8813)
                     /gene="metE"
                     /locus_tag="PK077_01385"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HRN22987.1"
                     /translation="MKTSVAGYPRIGSSRELKVAVEQYFRGKMAPEALLETGSRLRRT
                     HWQKQMEAGIDGIPSNDFSFYDQMLDTAVLLNAVPQRYRDLGISSLDTYFAMARGYQG
                     PAGDVKALAMKKWFNTNYHYLVPEIDSAPLQISGSKPFDEFLEARSYGIETKPVLIGP
                     FTFLTLSSLAGGRTRESVAGELARAYAAILARFHELDAAWVQLDEPALVRDLDRQDID
                     LFLRLYESMLPSKGRVKVLLQTYFGDIRDCYEQVAGLDIDAVGLDFVEGKQSLSLVKE
                     YGFPKDKLLLAGVVNGKNIWRNHYSRTLALLADLKKTGARIGIGTSCSLLHVPYTVAQ
                     ETKLPEYALKHFSFAEEKLQELRDLSFLFSLENAEPEKIYQVNDALFQSDRIGKNAAV
                     QAEVFALKPDDFTRFPSFEEREKLQKTRFRLPLFPTTTIGSFPQTAEVRSNRAAFRKN
                     LICGEQYRQFNFDRIKECISLQEKIGLDVLVHGEFERNDMVEYFGEHLQGFLFTEKAW
                     VQSYGTRCVKPPIVWEDVSWMRPITVEYAVYAQSLTNKPVKGMLTGPVTILNWSFPRE
                     DVSLEEQALQIALAVRKEVLALEEHGIGIIQIDEAALKEKLPLRRSDWHGEYLDWAIP
                     SFRLVHSGVRPETQIHTHMCYSEFAAIIQEIDSMDADVITFEASRSNLDILDALKECG
                     FKTEIGPGVYDIHSPRIPGETEIMENLHRMLRKILPEKLWVNPDCGLKTRGNEETIGS
                     LKNMTAAARALRTEFQS"
     gene            8992..9138
                     /locus_tag="PK077_01390"
     CDS             8992..9138
                     /locus_tag="PK077_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin"
                     /protein_id="HRN22988.1"
                     /translation="MNIKSALLTLAAALALSSCNTISGIGKDVEAMGSKIDSASQATS
                     KSMQ"
     gene            complement(9408..9551)
                     /locus_tag="PK077_01395"
     CDS             complement(9408..9551)
                     /locus_tag="PK077_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin"
                     /protein_id="HRN22989.1"
                     /translation="MKSKLIILSIATLLGCVSCNTIGGVGKDVEAVGSDINSAARSTS
                     RAM"
     gene            9753..10634
                     /locus_tag="PK077_01400"
     CDS             9753..10634
                     /locus_tag="PK077_01400"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HRN22990.1"
                     /translation="MIKVAIVGYGNIGKYAVDALRAAPDMELAGIVRRPGSEAVHGIK
                     TVSDVEELGHVDAALLCTPTRSVEETALPLLARGINTVDSFDIHGDIVALRRSLGAQA
                     IKHDAVSIISAGWDPGTDSVIRTLMLAMAPKGITYTNFGPGMSMGHSVVARSKEGVAD
                     ALSLTIPTGSGVHRRMVYVVLKEGAKFSDVEFAIKSDSYFSHDDTRVLQVPDIDVLKD
                     MGHGVLMERKGVSGSTQNQMFTFEMRINNPALTAQVMVASARASMKLAAGCYTLPEIA
                     PMDFLPGDREELIAQLV"
     gene            complement(10729..11463)
                     /locus_tag="PK077_01405"
     CDS             complement(10729..11463)
                     /locus_tag="PK077_01405"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase family protein"
                     /protein_id="HRN22991.1"
                     /translation="MKKNVVLSLLGLVLLVSAVAVFNMACAEKSSNRADIARKRVTPV
                     LNSFLEPCGARVGSPVFLRAVKEDSVLELWVKPDKSECYMLAKRYPIAAWSGKLGPKE
                     KEGDRQTPEGFYEVEPSGLNPRSNYHLSFNIGYPNAYDRSLNRTGSFIMVHGRDVSIG
                     CLAMTDSGIEEIYTMVEQALVHGQKNVPVQIYPFVPTPARLSEEKDSPHAAFWADMAR
                     AWDWTERTRTPARVKVVNGRLVVVEQ"
     gene            11980..12144
                     /locus_tag="PK077_01410"
     CDS             11980..12144
                     /locus_tag="PK077_01410"
                     /inference="COORDINATES: protein motif:HMM:NF024769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRN22992.1"
                     /translation="MAFPRLYRHVRLDTIQIKKNQPELFCKRLEKFLKNTRENRMYGE
                     WHDGGRLLEK"
     gene            complement(12288..17411)
                     /locus_tag="PK077_01415"
     CDS             complement(12288..17411)
                     /locus_tag="PK077_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HRN22993.1"
                     /translation="MGGLIVEEGASGFSLVPNNNAQRTLILGRAGTEEPVLFTVREDF
                     SLGSSSNAWGPVNVNADWNVLVANGKTLNVYGALNSSGRTITVGGEGFSGTLVLNNAA
                     NASMTAEWVITSGSTVRCMNNSALGSGLVTLDGGTLDFNSQTIGSTITLNMSGTGTLQ
                     NVTVDGAILSYTSSSGNDGITASNTTWTSGKIDIGRLLQGFSAEGSNTVDLGAELGEG
                     FSVLGLDTGQYRIEGRPLIIEGEAISSVTWVSAGEGGELEETVKNAFTLALGEGRAAN
                     VSLGYLNGTLTTSGDKVYQITNTGETKINLSGVYNSGATLPSGNLNYQGDIWMDINGG
                     AFGIIAGGVTNEWNTKLQTSTLTGDTHVQLSGNATAEHVIGGNNKGASTTLTGNTNVT
                     VKDNAIVAGAIIGGSTSAHNAVTTITGNTSVLVTNVQYSNTAQNLDGGLSNSYIIGGS
                     SWSSNTTSGTTIQGSTSATINLNGITLSGTEEHNSFVKTIIGGSYGNVNNAGTVNNIN
                     GDTSVSIIGREGITFTGDIIGGSFENSGQAQYTIGGNSSISISGGSTFTGNIYGGSYS
                     KVPGNTGSTMTTAGNITVELGTGTYRGNIYGAGNKGTAGGDVLVSLTGGSVFGAEGEQ
                     SGITIGGSAGAAVEGNRTLELKGTFGDADFQYASFTQFDKIDVTGEGSSATIYSLTES
                     ARLEKTGAGRLTLGANAAGAETILGGITEGISITEGSLNLSGAGGSHMKGTWNIASGS
                     RLTGVSGTVTVGGENGLDRLSIALGTENIGRDTQASDAVISNGSGTGSDPNLSIEGEG
                     LTLDLSNDAVVNLLLDHKTDDSSSYLTLTSGTLTVGNLGDIAFTTDLLSNYGIRVTGT
                     NGGSLVLSGAATGLYRVQEEGGNAHEVNSYQTLSGYAGVVIGGGQTLTVNLAGAPGEA
                     DGQGAKINNLMGATGSSLAVNNTGDGTAVVILNNKQMTTGEDDIDPAGQDTVMGGSIT
                     GGSNVTFIKEGTGTLTVGGTMDVETLALREGNIILNGTENSLDTLTLEGGGLTISGNA
                     EIETITGTEAGGTLAIQGTLDLTGTSSINNGAITGTGSLRIREGAELALGGEARLDGT
                     SVTADGTLTLTGTESGAISGLSGSGALSMNGGSLSISSATTSSGTFSGTLAGSGTLDI
                     SGQATQYLQTGNKDYDLAVRDGGVLVLKGTADAPTLNYNSITAGNNGTLRIEATGDAQ
                     GSANTTLNVENITFQNGSTTELIYNFNQDAPFGAPMLTAGTITVQDGAGFLLSNMKGN
                     AAMNAGSDLHDVVLMSATGSISGLEDGQSLAARISGLFAVYYQDATLSRDGNDILLNA
                     TLRQENLFASAADTWNSAAGAGLLWEARKNLDPDSQLAQFMNGVSTMINDGNLSGASR
                     AMAAAAGSTVNALGTAQRDALRDQMGWIRNRTTLMGVNPAYVNDDLPRFHMWMEGTGS
                     YAKLDTRGDESGYQLTTWGGTVGVDADLSDRLTVGAAFTAGYGDLTAGAADSADGHLD
                     SYYASLFGRYQDRRWAHTLILTGGWNDAKLNRTVNYGEGSYGTQGSTSGWGFGAMYEL
                     TYDVYLNENRSSVLQPLFNASVVTTRMDGYEETGAGNAGLNVGRQDWTTGTLALGGRW
                     MGLVGSNIFGREALAEIRVNAAQDLGDRRGETNVSLLGNPGFAQSVRGAKTGTTALQL
                     GAGLSVPVGTKGTIYVNGNADIRDGSSSVNGSVGYRYDF"
BASE COUNT         3817 a         5094 c         4717 g         3932 t
ORIGIN      
        1 gctgaaaagc aggcggacaa agccgactct ccccaggccg ccgtctcccc ggcggctttt
       61 tgcgttcgcg ccaccgtcgt gaagacggta aaaaaagact ccggcaagaa tggatatgcc
      121 atcatcattt ccgaagaaac agcttcaatg ccggcctgga aggccgtggc ggacaaactg
      181 gctgaaaaac ccggaggctc catcgtcacc gtgaaagatt ccgtattctc caagctggac
      241 acgctcaaaa aaatggctcc acgtttcatg gccgtcgtcg cacgcccgga ggaaatagac
      301 cgggtgctgg tgaatgacct gcaccgcctg agccgccggc tggacgacga cccttacgga
      361 gactgtatct ggggcattgt cacaggatac accccccagg ccgccatgag aatagcctct
      421 gcaacgaagc ctctcgtcat cagccgcgcc atgggaacaa ccaatgtgga ttcctcccgg
      481 ttcaaagaca gcatgagcat cacggactgg cagcccttcc aatacctgga acagcatggt
      541 tccaagggca aagtgacgcc ggctttctac acgaaaggcc tgaaagaaca ggacaagggg
      601 gatgaaacca cgctgggcgt aacgcccaag cttatggaat actggaaacg ttattccccg
      661 cagctcttcg taacggcctc ccacgcaacc cagtttaatc tggaaatgcc cttcggcaag
      721 ggcctcatcg tttccggtaa caaccggttc tacgcgctgg acaaaaaaca gttcagggaa
      781 ttcaccactt ttttgcgcgg cgtgctcttc aacggaaagg aagacgacct gctctccttc
      841 cttaatagaa ttaaagcgcc ggtgattgaa accaggccgg ttccggcagt ctgggtcgcg
      901 gcggggaact gcttgatcgg agatgccaag aagacgaaaa attccatggc tgtcaccgcc
      961 ctgagccgct acggtttcaa ccagctggta ggctacaccg tcccttcctg gtatggcaag
     1021 ggcggctggg ggacgctagg ccttctgttc agcaaccatg acgcctccag cctggcggaa
     1081 gcctggtatt tgaacaacca gttcattctg gatgagacca tgacccgctt ccccaagctc
     1141 atggacgtcc atttcaactc cccggatatc aatggaatca agaatgatcc ggactttgcc
     1201 aggggcatga actccgccgg atacggcatg ggcaaggacc agctggggct tatccacgat
     1261 cgggataccg tggcctttta cggagacccc gcctggacgg cgcgcctgga cgaatcccgg
     1321 gctccgtccc cgtggcatat cgactggaat gacccggcag atgcctccaa agggttcacc
     1381 gtcacagcca acaaagacgc caaaggtcgc ctgggagtct ggttccccaa tcggatcaac
     1441 gccggcaagg ccaccgtcac cattggagga actgccactc ccattgaaaa ggcaggcctg
     1501 ctgaccaacg acttcctgct gctccgggaa ctggaactca aaaagggaga aaaagccgtc
     1561 gtggaaatgg aataagcgtg gaagaaaaac aagttgcagg gatttttccc cttccgtcgg
     1621 cggccaaata tccggccgcc gctctttttc catagacaag cagcctccaa acatggatac
     1681 tcccgataat caatccaccc tatgactgat tactccattt tctttatcat cgccgtagta
     1741 gcgacgggcg tgggagcgct cctgtttctc ctgtccctgt tcgggctggg ggaacatgat
     1801 ctggactttg atgcggattc cggcggagcg gacgtagggt tgctgtccat caaatccggc
     1861 atcggctttt ttctgggctt cggctggggc ggcgtgctcg cccagggtct ggactggggg
     1921 atggccgctt ccctggcggc ggcttttttc accggcgtgc tgatgttcct gatcatcggc
     1981 atctccatgc gtttcattat gagccttaaa tcagacggca ccctgaatta tgaaaccctc
     2041 aagggaatga ccggctccgt ttacatcagc attcccggaa acctccaacg cggcggacaa
     2101 gttacaatcg cccatcccag ccagctgctg tacctccctg ccgttcagga aggggaacat
     2161 cccctgccct ccggaacccc ggtggaagtg gtagccgtca ctgcgggaat cgtcaccgtc
     2221 aaacctcttc attaacaaac accccaatca tcatacccca agacgactat ggacaaaatc
     2281 atccccatcg ccattctggt tctcttcatc attctgaccg cttcctggct gttcagccgc
     2341 taccgcatgt gccctccgga caaaatcctc atcgtcttcg ggaaagtagg cacaggccag
     2401 ccggccaaat gttaccacgg cggctccacc ttcgtgcttc cggttctcca gtcctatagc
     2461 tacctggatc taaaccctat caatattgac gtgcccctcc aaggagccct ttcctctcag
     2521 aacatacgcg tggatgtacc ctcctccttc atcgtgggca tctccaccct tcctgaaatc
     2581 atgcagaacg ccgctgcgcg cctgctgggc cgttcccggg aagaaatccg caacctggcc
     2641 gcggaaatca tcatggggca gatgcgcgtg gtgattgcct ccatgaccat tgaggaaatc
     2701 aactccgacc gcgaaaaact catcaagggc atcacggaag gcgtggacgt ggaactgcac
     2761 aaagtgggcc tccatctcat caacgccaat atcacggaca tccaggacgc ttccggctac
     2821 atcaacgccc tgggcaagga agccgctgcg cgcgccatca atgacgccac catcaaagtg
     2881 gcggaagaaa cgcgccgcgg ggaaatcggc aaagcggaag cggaaaagga ccagaccatc
     2941 caggtggcga acgcccgcgc catcgccatt gaaggccaga acgaagccca gattaaaatt
     3001 gcggaatccg ccgccaagct gcaagtgaag caggcggagg ccaaaaagct ggcggaagtg
     3061 gcccagaagg tgcaggaggc taaaaccctg gaagaagcct acgaggcgga aaaggaagcg
     3121 gaattaaagc gcgcggaacg cgaacgcgcc acgcaggaag ccaacatcct ggtaacggcc
     3181 cgcattgaaa agagccagcg cgaagtgcag gcccaggcca cggcggaagt actcaaactg
     3241 gaacaggaag gcaaggcgca ggctctgctc atccagcgca gggcggaagc ggaagccatc
     3301 cgccaactgg cggatggcga agcccaggct accctcctga agaaaaaggc ggaaggggaa
     3361 ggcatggaaa tggtgggacg cggtgaagcg gccgcgattg aagccgtgct ggaaggcaag
     3421 gcccgcggtt ttcagcagat cgtccaggcg gcaggttctt ccgaggccgc ttccaacctg
     3481 ctggtgacgg aacagcttac caagattgtg gagctccagt ccggtgccat taaggggttg
     3541 aaattcgaca aggtagtcgt catgggcaat ggagggaact cctctgtggg aggattcgtc
     3601 cagaatctgg taacggatac gctgcccctt catgaactcg gcaaaagcgt agggctggaa
     3661 ttgcccgctt ttctgggcaa acccatggaa gggaaaaccg cagcttcctc ccctgctccc
     3721 gcagccgatg ccccggcggc agataccgca accaccgtca acccgagtta acccctccat
     3781 ccccgccttc ctgacggcaa ccgccccgcc acccagggaa gcttccgaac ggtgttccgg
     3841 gaattcccat ggcagggagg agcggccgcc tgacaacctc tcccgtagcc gcacagtcga
     3901 aacaagcggc ataccgcatt ccgatcatag agaataagcc ggacatgccg gtgaacccgc
     3961 aggacaggga ggcactagac agccttgttc atattgacgc tggacgcatg ccgcagcgga
     4021 aaagcccact taccgccaga aggtaaacag agtttgaaac atgaaaagag aatccgaatg
     4081 cctgccttca aacctaacgg gcagatggcg aaacgccata ttgattcatt atccgtcatt
     4141 gagtattgtt gattttccct gctcatggta catatccggg cacgcatgag tgttctgctt
     4201 catatctgct gtgcgccctg ttccatttcc tgcattgaaa tgctgcggga agaaggaatg
     4261 gaaccaaccg gattctggtt taatccaaat attcatccgt ataaggaata ccaggccaga
     4321 aaggatacgc tggtcgaata cgcgacaaaa atcggtctga aattgattat gagggatgaa
     4381 tatggactgc ggcccttcat tcgcgccatt tacccctccc tggaaaaccg ttgcggaacg
     4441 tgttacagga tacgttttga agagactgca cggtttgccg cagaaaacgg ggtcgacggc
     4501 ttttcttcaa ccctgttcgt cagcccttac cagaatcatg aattactgag aaagaccgct
     4561 gaaaaaacgg cagagaaatt tggaacccgt ttcatttacc gtgatttccg ccccggtttc
     4621 agagaaggtc agcaaaaggc cagggaactc cggctgtaca tgcagaagta ttgcggatgc
     4681 gtcttcagcg aagaggaacg ttacctgaaa aagaaataac tccattttac caggcttttg
     4741 ccttttcccc atgaccctgc agcagctaaa atatatcatc ggcatcgtca acagcggttc
     4801 catcaatgag gctgccaaac ggcttttcat atcccaaccc agcttgtcca aagcggtccg
     4861 ggaactggaa aatgaattga attttgagat cttccgccgt tcttccaagg gcatttccct
     4921 gtctttggaa gggacggaat tcctttccta cgcgcgccaa gttgtggaac aggcggaact
     4981 tctggaacag cgttacctga atacaaaacc tctcaaacgc caattttcca tctcgactca
     5041 gcactacgct ttcgccgtca atgctttcgt caatctcatc aaaaaactga acatccagga
     5101 gtacgaatgg acactgagag aaacgagaac cttcgagatc attgaagatg tccatcaatc
     5161 ccggagcgac ctgggtatcc tgtatctgaa tgatttcaac aggcaggtta tcagcaagct
     5221 tctcaaaaag aacgatctct gtttccatcc ccttttccgg gcagaaccgc acgtctttgt
     5281 cagttcggca catcccctgg cgcgccgcag gcaggtttta ctggaagatc tggaagatta
     5341 cccctgcctc agttttgagc agggaaccta taattccttt tatttttcag aagaaattct
     5401 cagcaccatc gttcacagga aggaaatcca tgtcagcgac cgggcgacct tgttcaatct
     5461 tctgataggg ctgaacggct ataccatctg ttccggagtt ctcagcgagg atttgaacgg
     5521 ctcgaacatt atttccatac cgctcaaaac caaggaatac atgctggtcg gatggattga
     5581 cagggaacaa tcccgcccaa gccgttcagc ccttctatat atagaagagc tgaaacgggt
     5641 tatcaccgaa tacggctatg aagtaatccg gactgacgat taattccgga tgatggaggc
     5701 ggtagcttcg caaatacggc cggcgacata cgggttattc atcgtataca ggtgaatgcc
     5761 atctactcct tgcgctacga gatcgataat ctgctcgacg gcgtaggcga tgccggcatc
     5821 ccgcattgcg atggggttat gctcaaacct ttccatcata cgtataaatt tcttcggcaa
     5881 atggacatgg cacagggaaa ccatcctctc tatctgcttt ttattgacga cgggcatgat
     5941 tcccgcttca agagggacgg aaatgcctgc cagatccagt ttttcccggt aggcgtgaaa
     6001 aatatcgttg ccaaagaaca actgtgaaat gagatggtcc gctccggcct ccaccttggt
     6061 cttgaggtgg cgaatatcat cgactatgct ttttgattcg ggatggcctt ccggatagca
     6121 ggccgccaga atgtgaaaat ttccctgttc cttaataaag gaaatgagat cgctggcata
     6181 ggtgaaatca cggcacggtt ctctgtccgg gggcaaatca ccgcgaaggg ccaggatatt
     6241 ttcaattccg ttccggcgga actgttccag aagctccaac acatttccct tcgtcaagct
     6301 gatgcaggga agatgggcta cgctctcaac catataatcc ttcttgatgg aggaggcgat
     6361 gttgagcgta tcgctggttc ccgcgcctcc cgaagcacca taggtcacgc tgataaaatc
     6421 cgggttcaat ccctgcaaag cgctcagagc atggtaaacc gtcccgatcg gggaagtccg
     6481 cttgggagga aacacctcaa aggaaagaac cctcttttgg ggaaacaatt cggaaagctt
     6541 catgactgga attccgtacg cagggctctg gccgccgccg tcatattttt gaggctgcct
     6601 atcgtttcct cattgccgcg cgtctttaac ccgcaatcgg gattgaccca cagtttttcc
     6661 ggtaaaatct tccgtagcat gcgatggaga ttttccataa tttccgtctc tccgggaata
     6721 cggggcgaat ggatatcata aactccgggg ccgatttcag tcttaaaccc gcattctttc
     6781 aaggcatcga gaatgtccag attggaacgg gaagcttcga atgtgatgac atcggcatcc
     6841 atggaatcga tttcctggat aatagccgca aattcgctat agcacatgtg cgtatgaatt
     6901 tgcgtttccg gcctgacccc gctatgcacg aggcggaacg agggaatggc ccagtccagg
     6961 tactctccat gccagtcgga acgacgcaga ggcaattttt ctttcagggc tgcttcatct
     7021 atctggatga tgcctatgcc atgctcttcc aatgccagca cctccttgcg gacagccaga
     7081 gcaatctgca gggcttgctc ctccagggaa acgtcttctc tggggaaaga ccagttcagg
     7141 atcgtgaccg gccccgtcag catgcccttg accggtttgt ttgtcaggct ttgggcatag
     7201 acggcgtact ccacggtaat ggggcgcatc caggaaacgt cctcccagac gatgggcggc
     7261 ttgacgcacc tggttccgta ggactgtacc caggcttttt ccgtaaaaag gaagccctgc
     7321 aggtgctcgc cgaagtattc caccatgtca ttgcgttcaa attctccatg gacgagaaca
     7381 tccagtccta tcttttcctg cagggaaatg cattctttaa tcctgtcaaa gttgaattgc
     7441 ctgtactgct ccccgcaaat gaggttcttc ctgaatgccg cccggttgga gcggacttcc
     7501 gcagtctggg ggaaggaacc tattgtcgtc gtaggaaaca ggggaaggcg gaaacgggtt
     7561 ttctgaagct tttcccgctc ttcaaaagac gggaatctcg taaaatcgtc aggcttcaag
     7621 gcgaatactt ccgcctggac agcagcattt tttcctatcc tgtccgactg aaacaaagca
     7681 tcattgactt gataaatctt ttctggctcc gcgttctcca gcgaaaaaag aaagctaagg
     7741 tcccggagct cctgcagttt ttcttcggca aaagagaagt gtttcaaagc atattccggc
     7801 agtttcgtct cctgagcgac cgtataggga acatgcagaa gggagcagga agtccctatg
     7861 cctatgcggg cacccgtttt tttcaaatcg gccagcaacg ccagagtccg ggaataatga
     7921 ttgcgccaga tattcttccc gttgacgacg cctgcaagaa gaagcttatc tttcgggaaa
     7981 ccatattctt tcaccaggga cagcgactgc tttccttcca caaaatccag tcctaccgca
     8041 tcaatatcca atccggcaac ctgttcatag cagtcgcgga tatctccgaa gtaggtttga
     8101 agaaggacct tcacccgccc ctttgaaggc agcatggatt catacaagcg gagaaacagg
     8161 tcgatatcct gcctgtccag gtcacggaca agagcgggct cgtccaactg cacccatgcc
     8221 gcgtccagct catggaaacg ggccagaata gcggcatacg cccgggccaa ctccccggcg
     8281 acactctccc gcgtgcgtcc ccccgccaat gaggaaagcg ttaagaatgt aaacggtcca
     8341 atcaggacgg gtttcgtttc aatcccgtat gaccgggctt caaggaattc gtcaaagggt
     8401 ttactcccgg aaatctgaag gggggcggaa tctatctcgg gaaccaggta atggtaattg
     8461 gtattaaacc attttttcat ggctagagcc ttgacatccc cagccggccc ctgatagccg
     8521 cgagccatcg cgaaataagt atccaatgaa gaaattccca aatcccggta ccgctgggga
     8581 acagcattta acaaaaccgc agtatctagc atctggtcgt aaaaagaaaa atcattggaa
     8641 gggatgccgt caatgcccgc ctccatttgt ttttgccagt gcgtcctgcg aaggcggctt
     8701 cccgtctcca acaacgcctc cggtgccatt ttcccccgga aatactgttc cacggcaact
     8761 ttgagttccc gtgacgagcc tattctggga tatcctgcaa ctgatgtttt catggtattt
     8821 ttcaatctgt aatgaaatgc aggcttctta tagcaaatag cgttattatt cgataataca
     8881 attattctat ggcataatat aacaaaaagc tataacaaac tactgcttgc aggttcattg
     8941 acaagaaacc ggaagcaggt taggatacgg ctttcaacct ctcttcgact aatgaatatc
     9001 aaatccgccc ttttgacgct cgccgcagcc ctcgctctct cctcctgcaa tacgatcagc
     9061 ggaataggaa aagacgtgga agccatggga agcaagattg acagcgcctc ccaggccacc
     9121 agcaagtcca tgcaataagg cccgggaagt gccggacgct gctttaagcc ctcttttaat
     9181 ggaaaagagg cagtactttg cttcctgcca ttctgccctg aatggcagtc atccccatgg
     9241 ggttcccaac gaattggcaa ggtccggctc catcctgcat ggaacacgcc aaacctttgc
     9301 ggccacccgg aaatcagggc atgcgggcgg cagcacattt tttactggaa agccgcttca
     9361 tccacccgga tgaagcggct ttcgtccaag gtctctacat gaccttttta catggcccgg
     9421 ctggtggaac gggcggcgga attaatgtcg ctgccgaccg cttccacatc cttgcccacc
     9481 ccgcctatcg tattgcagga aacgcacccc agaagggtag cgatactcag aataatcaat
     9541 ttgcttttca tcgttttatt ggaagagttc cgagtaatca gactctattc ctccgccccc
     9601 cgttcaaaga accggcaatc atgccgcaaa gccccctgtc attctccgtt cttgccatgc
     9661 gttcccaaaa cacttaaaaa acatgcgcca aaggaaaaaa atgccgggcg gacattccgc
     9721 cccattagga accattacta tttactacca agatgattaa agtagccatc gtaggatacg
     9781 gcaacatcgg gaaatacgct gtggacgcct tgcgcgccgc tcccgatatg gaactggcag
     9841 gtatcgtgcg ccgccccggc agcgaagctg tacacggcat caagaccgta agcgacgtgg
     9901 aggaactggg ccatgtggac gcggccctgc tctgcacccc cacccgcagc gtggaagaaa
     9961 cggctctgcc cctgctcgcc cgcggcatca acacagttga cagcttcgat attcacgggg
    10021 acatcgtggc cctgcgccgt tccctgggag cccaggcaat caagcatgac gccgtctcca
    10081 tcatttccgc cggctgggat cccggaacgg actccgtcat ccgcaccctg atgctggcca
    10141 tggctcccaa aggcatcacg tacaccaact tcggccccgg catgagcatg ggccacagcg
    10201 tcgtggcgcg ttccaaggaa ggagtggcgg acgctctttc cctcaccatc cccacgggtt
    10261 ccggcgtaca ccgccgcatg gtttatgtgg tgctgaaaga aggcgccaaa ttctccgacg
    10321 tggaattcgc cattaaatcc gattcctact tcagccatga cgacacccgc gtgctgcagg
    10381 tgcccgacat cgacgtgctg aaagacatgg gccacggcgt gctgatggaa cgcaagggcg
    10441 tttccggctc cacgcagaac cagatgttca catttgaaat gcgcatcaac aatccggctc
    10501 tgaccgccca ggtcatggtg gcttccgccc gcgccagcat gaaactggcc gcaggctgct
    10561 atacgctgcc ggaaattgcc ccgatggatt ttctgccggg cgaccgcgaa gaattgattg
    10621 cccagctggt ctaatccgga gcattccgga cacacggtgc ccggggcgct cctgtcgccc
    10681 ctgttccgca ggccttcctt tatcctccgg tcctcaaccg gaggattttt attgctccac
    10741 aacgaccagc ctaccattga cgaccttcac ccgggccgga gtacgggtcc gttccgtcca
    10801 gtcccacgct cgggccatgt ccgcccaaaa ggcggcgtgg ggagaatcct tttcctctga
    10861 caaacgcgcc ggagtgggca cgaagggata aatctgcacg ggaacgtttt tttgcccatg
    10921 aaccagagcc tgttccacca tggtataaat ctcctcaatt ccggaatcgg tcatcgccag
    10981 acagccgatg gaaacatccc ggccatgcac catgatgaag cttcccgtcc ggttcaggga
    11041 gcggtcatag gcattcggat acccgatgtt gaaagacaaa tggtaattgc tccggggatt
    11101 aagcccggac ggttccactt cataaaaacc ttccggtgtt tgcctgtccc cctccttctc
    11161 cttgggaccc agttttccgg accatgcggc aatcggatag cgcttggcaa gcatgtagca
    11221 ttccgatttg tccggtttca cccacagttc caggacggag tcttccttta cggcccgtaa
    11281 aaacacagga cttcccaccc tggcgccgca cggctccaga aaggaattca gcacgggcgt
    11341 tacccgtttc cgcgcgatat ccgcacggtt ggaggatttt tctgcacatg ccatattgaa
    11401 aaccgctacg gcggaaacca gcagaaccag ccccagcaag gacaagacta cattcttctt
    11461 cataaatcat ctataatggc ggcttttgtt ttgacaagca gaaaaccagc cgtttctctc
    11521 cgaagaagga aaaggaaaaa acggcggaaa aagaagacgg aaagaaaagc ggttcctgag
    11581 aaaggcgccc ttcccgaagc tgtccccggt tagaagccat gtctgtgctg gaaatgcaag
    11641 ctccggagaa gtattatcgg ccctcagaac atcagcggaa gaccgaccta taaacgccat
    11701 ggaaataagc aaccttcttc ccatcactac acggaaggaa ttaagaatat ggttggaaaa
    11761 caaccacctc acttcctcct gctgctgggt tctcacaacg aggaaacctt ccccacatgc
    11821 cctcctttac ctggatgccg tggaggaagc cctgtgcttc ggatggattg acagcacccg
    11881 caagaagacg gcctccggtt tcctggccca gcgtctcact ccccgaactc gtatatttta
    11941 cagctccttc aacaggatcc gcttatttac gccaatttcc tggcgtttcc ccggctatac
    12001 cgccatgtgc gcctggatac cattcaaatt aaaaaaaacc agccggaact gttctgcaag
    12061 cgcctggaaa aatttctgaa gaatacccgt gaaaacagga tgtacggaga atggcacgac
    12121 ggaggacggc tgctggaaaa gtagacgcca ggatcccgcc atttccctgc cggacgagct
    12181 ctgacgttac caggccgttc cggaataaat ccggaacggc tctgaagggc caggaggaag
    12241 gcttttcggg ggtgggtggg gagccttctc cttgggtttt ctttctttta gaagtcgtag
    12301 cggtagccga cgcttccgtt gaccgagctg gacccgtcac ggatgtccgc gttcccgttc
    12361 acgtagatgg ttcccttcgt tcccaccggc acgcttaacc ccgctcccag ctgcagcgcc
    12421 gtcgttcccg ttttcgcccc tctcacgctt tgcgcgaagc cggggttgcc cagcagggag
    12481 acgttcgttt cccctctccg gtctcccagg tcctgcgccg cgttgacccg gatttccgcc
    12541 agcgcttctc gtccgaagat gttgctgcct accaggccca tccaccggcc gcccagcgcc
    12601 agcgtccccg tcgtccagtc ctgtctgccg acgttcaggc ccgcgttgcc cgcacccgtt
    12661 tcctcatagc cgtccatccg cgtcgtcacc accgaggcgt tgaacagcgg ctgcagcacg
    12721 ctgctgcggt tttcgttgag gtatacgtcg taggtgagtt catacatcgc tccaaagccc
    12781 cacccgctgg tgcttccctg cgtcccgtag cttccttccc catagttgac cgtacggttg
    12841 agtttcgcgt cgttccaccc tcccgtcagg atgagcgtgt gcgcccagcg cctgtcctgg
    12901 tagcggccga agaggctggc gtagtagctg tccaggtgcc cgtcggcgct gtccgccgcg
    12961 ccggccgtca ggtcgccgta gccggccgtg aaggccgctc ccaccgtgag gcggtcgctg
    13021 aggtccgcgt ccacgcctac cgtgcctccc caggtggtga gctggtagcc gctttcatcc
    13081 ccgcgggtgt cgagtttggc gtaggagccc gtgccttcca tccacatgtg gaagcggggg
    13141 aggtcgtcgt tgacgtaggc cgggttgacg cccatgaggg tggtccggtt cctgatccag
    13201 cccatctggt cgcgcagggc gtccctctgt gccgtcccaa gcgcgttgac cgtgctccca
    13261 gccgccgccg ccatggcgcg ggaggctccg gagaggttgc cgtcattgat catggtgctg
    13321 acgccgttca tgaattgggc cagctgggag tcggggtcca ggtttttgcg ggcttcccag
    13381 agcaggccgg ctccggcggc ggagttccac gtgtcggccg cggaggcgaa gaggttttcc
    13441 tgccggagtg tggcattgag caggatgtcg tttccgtcgc ggctcagtgt ggcgtcctgg
    13501 tagtagacgg cgaagaggcc ggatatccgg gcggcgaggc tctgcccgtc ttccaggccg
    13561 ctgatgctgc cggtagcgct catcaggacg acgtcatgga ggtcgctccc cgcgttcatg
    13621 gcggcgtttc ctttcatgtt ggagaggagg aagccggctc cgtcctgcac ggtgatggtg
    13681 cccgccgtca gcatgggggc gccaaagggg gcgtcctggt tgaagttgta gatcagttcc
    13741 gtcgtggagc cgttctggaa ggtgatgttt tccacgttga gcgtggtgtt ggcgctgccc
    13801 tgggcgtccc cggtggcttc aatacgcagg gtgccgttgt ttccggcggt gatgctgttg
    13861 tagttcagcg tgggggcgtc cgcggtgcct ttcaggacca ggacgcctcc gtcacggacg
    13921 gcgaggtcgt agtccttatt gccggtttgc aggtattggg tggcttgccc ggagatgtcc
    13981 agggtgccgc tgcctgccag cgtgccggag aaggttccgg aggaggttgt cgccgaggag
    14041 atggagaggc tgcctccgtt catggacagg gcgccgcttc cggagaggcc gctgatggcg
    14101 ccggattccg ttccggtgag ggtcagcgtg ccgtcggcgg tgactgaggt tccgtccagc
    14161 cgggcttctc cgcccagggc cagttccgct ccttcccgga tgcggaggga gccggttccg
    14221 gtgatggctc cgttgttgat gctgcttgtt ccggtcaggt caagagttcc ctgaatggcc
    14281 agggttcctc cggcttccgt gccggtgatg gtttcgattt cggcgttgcc gctgatggtc
    14341 aggcctcctc cttccagcgt gagggtgtcc agggagtttt ccgtgccgtt gaggatgatg
    14401 tttccttctc gcagggcgag ggtttccacg tccatcgttc ctccgaccgt cagcgtcccg
    14461 gttccttcct tgatgaaggt gacgttgctg ccgcccgtga tgctgccgcc catgacggtg
    14521 tcctggccgg ccgggtcgat gtcgtcttct cccgtcgtca tttgtttgtt gttgaggatg
    14581 acgacggccg tgccgtctcc ggtgttgttg acggccaggc tgctgcccgt ggctcccatc
    14641 aggttgttga ttttagctcc ctgtccgtcg gcttcgcccg gggctccggc caggttgacc
    14701 gtcagggtct ggccgccgcc gatgacgacg ccggcgtagc cggagagcgt ttgataggag
    14761 ttgacttcat gggcgtttcc tccttcctcc tgcacgcggt agaggccggt ggccgctccg
    14821 ctgagcacca ggctgcctcc gttcgttccg gtgacccgga tgccgtagtt ggacagaagg
    14881 tcggtcgtga aggcgatgtc gcccaggttg ccgacggtca gcgttccgct ggtcagggtc
    14941 aggtagctgg aggagtcgtc ggttttgtgg tccagcagga ggttgacgac ggcgtcgttg
    15001 ctcaggtcca gcgtcagccc ttccccctcg atggacaggt tcgggtcgct tcccgttccg
    15061 ctgccgttgc tgatgacggc gtcagacgcc tgcgtgtccc ggccgatgtt ttccgttccc
    15121 agggcaatgg acagtctgtc cagaccgttt tccccaccca cggtgacggt tccgctgact
    15181 ccggtgaggc gggagccgga ggcgatgttc cacgttcctt tcatatggct gccgccggct
    15241 ccggagaggt tcaggctgcc ttccgtgatg gaaatgcctt ccgtgattcc gccgaggatg
    15301 gtttccgccc ctgcggcgtt agcccccagc gtcaggcgac ccgccccggt tttttccagc
    15361 ctggcgcttt ccgtcaggga ataaatggta gcggaggagc cttctccggt aacgtcaatc
    15421 ttgtcaaatt gagtgaagga ggcgtactgg aagtccgcgt ctccaaaggt tcccttgagc
    15481 tccagcgtcc tgtttccttc gaccgccgct ccggcgcttc cgccgatggt gatgccgctt
    15541 tgttcccctt ccgccccaaa gacgcttcct cccgtcaggg agacgaggac gtcgcctcct
    15601 gccgtgcctt tatttcctgc tccataaatg tttccccggt aggtgcctgt tccgagttct
    15661 accgtgatat tgcctgcagt ggtcatggtg ctgcctgtgt ttcctggcac tttggagtag
    15721 gagccaccgt agatgttgcc ggtgaaggtg gagcctccgc taatggagat ggaggaattt
    15781 cctccaatcg tgtattgggc ctggcctgaa ttttcaaagg aacccccaat gatgtccccc
    15841 gtgaaggtga ttccttccct gccgatgatg gaaacggagg tgtctccgtt gatgttgttg
    15901 accgttccgg cgttgtttac attgccataa ctgccgccaa tgattgtttt gacgaagctg
    15961 ttgtgttcct ctgttccgga aagcgtgatt ccgttcaggt tgattgtagc cgacgtgctg
    16021 ccttgaatcg ttgttccgga agtggtattg cttgaccagg agcttcctcc gatgatgtat
    16081 gagttggaga gtcccccatc caggttctgg gccgtgttgg aatattggac attggttacc
    16141 agcacgctcg tgttgcccgt gatggtggtg acggcgttgt gggcggaggt gctcccgccg
    16201 atgatggctc ccgccacgat ggcgttatcc ttgacggtaa cgttggtgtt tcccgttaat
    16261 gttgtagacg ctcccttgtt gttcccgccg atgacgtgtt ccgccgtggc gtttccggaa
    16321 agctgcacat gggtgtcccc agtcagcgtg ctggtctgga gtttggtgtt ccattcattg
    16381 gtgacacctc cggcgatgat gccgaatgct cctccgttga tgtccatcca gatgtccccc
    16441 tggtagttga ggttgccgga cggcagggtc gccccgctgt tgtatacccc ggacaggttg
    16501 attttagttt cgccggtgtt ggtgatttga tagactttgt ccccgctggt cgtcagggtc
    16561 ccgttcagat atcccaggct gacgttggcc gctctcccct ccccaagagc cagcgtaaag
    16621 gcgttcttga ccgtttcttc caattcccct ccttctcccg cagataccca cgtcacgctg
    16681 gatatcgcct cgccttctat tatcagcggg cggccttcta ttctgtattg ccctgtatct
    16741 aagcccagta cgctaaagcc ctcgccaagc tccgctccca aatcaacggt attggaacct
    16801 tcggcactaa atccctgcaa caaacgccca atatctattt tcccggacgt ccacgtggta
    16861 tttgaagcag taatcccatc atttcccgat gaagacgtat aagataaaat tgctccatca
    16921 actgtcacat tctgcaatgt cccggtcccg ctcatgttca acgtaatggt tgatccgatc
    16981 gtttgcgagt taaaatcaag cgtcccccca tccagcgtaa caaggccgct tcccaaagcg
    17041 gaattattca tgcaccttac agtagagccg gaggtgatga cccattcagc agtcatggat
    17101 gcattggccg cattgtttaa aaccaacgta ccgctgaatc cttccccgcc caccgtaatg
    17161 gtccggccgg aagaattcag tgccccgtaa acattcagcg tcttgccatt tgccacaagg
    17221 acattccagt ctgcattcac attaaccggg ccccatgcat tggaggaaga acccagggaa
    17281 aaatcttcac gaaccgtaaa taataccggc tcttccgtac ccgctctccc cagtataaga
    17341 gtcctctggg cattattgtt cgggacaagg gagaaccctg atgccccttc ctccacaatc
    17401 aatcccccca tggtaaatgg cgtaaaggag caggatggat ttttattggt cgccgatacg
    17461 gtgacatcgc ccacttccgt gcctgatgcg acaaagcgca atgtgttata ttgcgacagt
    17521 cccgtttcat tggcaaaaag gttccaaata ctcccctcct
//
LOCUS       DAOMSU010000015        17388 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_107_length_17388_cov_2.846593, whole genome shotgun sequence.
ACCESSION   DAOMSU010000015 DAOMSU010000000
VERSION     DAOMSU010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 17388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17388
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107_length_17388_cov_2.846593"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PK077_01420"
     CDS             complement(<1..466)
                     /locus_tag="PK077_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRN22994.1"
                     /translation="MSDFLKHECGVAAIRLLKPLSYFQDKYGSTLWAFNKLLILMEKQ
                     YNRGQDGAGIGCVKLNMPLGQPYLFRTRDASKDALTSIFNGQIKKLNKKVRRGLVNLK
                     DAADIKNNFDYGGEILMGHLRYGTSGLFDEGSCHPYLRRTNWPTRTLMVLGNF"
     gene            complement(529..1464)
                     /gene="purM"
                     /locus_tag="PK077_01425"
     CDS             complement(529..1464)
                     /gene="purM"
                     /locus_tag="PK077_01425"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HRN22995.1"
                     /translation="MYPTSALTLKERKNLHQAFGLFAAAYDLSSYKEPVIVTGCDGVG
                     TKTEILFELDMVETAGKDLVAMNVNDILTTGGDPLLFLDYLGISNLERERSRITRLVA
                     GMCDYLESCNCILAGGETAEMPGVVPESIVELSGFCIGCCEKSKLIDPKTVRPGDVFI
                     GYKSDSFHANGWSLIRRILEENPDVVDEEELRSLLQPTRLYHDVVADMRRFNVTPKAY
                     AHITGGGLPENLERFLGDYGADLSIPYWDNTAAQKILKHVDPQDRFNTFNMGIGWVAI
                     VRPEDAEAALKAGPGGTVIGTLREGRGIHVKVQGE"
     gene            complement(1626..2087)
                     /locus_tag="PK077_01430"
     CDS             complement(1626..2087)
                     /locus_tag="PK077_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRN22996.1"
                     /translation="MEQNKSKSSACRLLGPLATNTSMDFGVLLLRLGVAVMMLVHGIP
                     KLGMLISGNWAAFQDPLGIGNFLSLLLCVGAEFGCSVLLLLGFLTRLAALILIINMCV
                     ALFLVLGLSGWGAQELAAIYLLIYLTIFYTGPGKFSLDAWWLRRDCKIEVE"
     gene            complement(2281..3258)
                     /locus_tag="PK077_01435"
     CDS             complement(2281..3258)
                     /locus_tag="PK077_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRN22997.1"
                     /translation="MNLHLILASLTATLSLGIAAQAAPAKVACVGDSITFGTGLKPGE
                     TRYPQVLATLMGPDFDVRGFGNPGKTAGNYPGQAGRWYGSTREHKQALEFKADIYICN
                     LGINDTGRWWNPELFSKGYDALLHAWKNANPKTRFFAWGLLGPDYRGPLNKKAFPGNC
                     YPDVRKYAGSDNGSSANRPEAEKLIAAVARKYKVSLFDALHPLSDHPEWYVDGLHPTE
                     QGARRIAEITFAKLAKSLRLKQPAPRLEPGTGNVIINNPGNSGILLDGWKLTDGTNTL
                     IFENSTVIHPKDRLIIAIGPETQKDPTKPLQIKSSQSPAAFRLIPAKKY"
     gene            complement(3280..4818)
                     /locus_tag="PK077_01440"
     CDS             complement(3280..4818)
                     /locus_tag="PK077_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRN22998.1"
                     /translation="MRLHTSCILAAAIALNAAWADAPRFDRIFGSHMVLPHGKNIPVS
                     GTAAPNREITVTFGNTALKTKTDSKGKWSVTLPPMPPCGTGQTLAAVQNGDSAKLEDV
                     LVGEVWLASGQSNMLFRLNQTTTAKEDIAASGDDQLRLLNNVPQAHTNNAPYSGKDFD
                     AVTTDNFYKGQWAASSPATSGPMTAVGYYFGKKLREGLGIPVGIIHSSLGGSEIAAWI
                     PRQVINANNSFRTLRGNHWLDSPLISDWVRGRARKNISPRLNQGSPDHPYKPAFLYES
                     GIAWTTALPITGVIWYQGESDAEIIDNAQNSMLLKTMISSWRKAFRNPEMPFVMIQLP
                     RINDPSKIRAGWPEFREMQDAVAKTVPKVYSVNTIDLGSTNSDVHPPFKRPVGERAGN
                     TALNKVYGKKIPCEGPALKAFKPSGSNILVQLDQAAGLTTTDGKEPAQFEIAGTDGTY
                     HPAAAEIINRKGNTAVIRLSSPEVKSPKHARYCWNRFVTPNLVNGNQLPARPFRTDEP
                     VLKK"
     gene            complement(4829..5605)
                     /locus_tag="PK077_01445"
     CDS             complement(4829..5605)
                     /locus_tag="PK077_01445"
                     /inference="COORDINATES: protein motif:HMM:NF041646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VC0807 family protein"
                     /protein_id="HRN22999.1"
                     /translation="MDKTPNKSQSSLLGIFINILLPVLILDYCSAGPANPLERPEGEN
                     FWHIGPVWALVIALSLPLVYGIRSLVVSRKFDLMSGVGMAGVLLTGVISIFVIGPEGR
                     IHSATPWLFAGKEALIPLILAAAVVVSRSTGTPLLNMFIYTPELFDVRRIEQTVAANG
                     EERAYQKLLANSSWILAGTLVASSIGNFFLSLSFMSSVIRQPEAEQQVAYNAAIGSIT
                     WWGFLIIGIPILAALVFIMTRLIKRLGRLTGLSRNELLLK"
     gene            5824..7638
                     /locus_tag="PK077_01450"
     CDS             5824..7638
                     /locus_tag="PK077_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRN23000.1"
                     /translation="MTQTSSFSALGISPEILEAIEVLGFDTPSSIQEQAIPAAIGGAD
                     IVGLSHTGSGKTLAFAIPALECIEPEERCVQVLALCPTRELAVQICREVDKLALFMDG
                     VSAVPIYGGSSFRPQLDALRRGVQFVVGTPGRVMDLMESGALRLDGLRMLILDEADEM
                     LDMGFLEDIERIADAMPEAKQTLFFSATMSPEISRLVSRFMKEPVQISIERPTLTVPT
                     IEQVYYEVVFSSRIEVLSRLLDTGKIKMGLVFANTKKVVDDVVDGLTARGYPVDRLHG
                     DMPQMMRERVMDSFRKGSLRLLVATDIAARGLDVDDVDAVVNFELPRDPEDYVHRIGR
                     TARAGRKGKAITFVGRRDFSLMSRIERFIGVKLTPEPVLTAVQVDQLRTESLVDDILE
                     RLKPDAVLPEELKEVEAAPEAMAAALFDLLRERTHREVQPIPEDKPARRARRMPQTGE
                     EAESPQKGDSWQHKGDTVTLFMNGGRMIGLRPKDIAGLFYNTVEMEKGAVGDIRIFPK
                     HSLIEVDASVAPLLLEALATATVCGYEVNVREDKGAPEYSGTPRSSRRNGGFRNSYRG
                     ERDRSDRSGFRGNRGGFRSDRGERWGERSRRDDRKKRF"
     gene            8057..10216
                     /locus_tag="PK077_01455"
     CDS             8057..10216
                     /locus_tag="PK077_01455"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRN23001.1"
                     /translation="MKLVSLLSVLLTSLVPCMGASGQATKPAARSVKTSKPNVIFILV
                     DDMGWGDLDSNWSQQKLNGRTVERKNEFKTPALSALAREGIQLRRHYSAAPVCAPARA
                     SLLLGVHQGNSRVVRNNRFDQPIEDSHTLGTVMRDAGYDTAAIGKWGVGGGGQSHVPM
                     TAGPHQRGFNYFYGILDHLAGHFHYPSESRDVFEYNGYASNPEWKNIKDQVPQTAYST
                     DLFAARAKQWIVDQRKSARKTGKPFFLYLAFPAPHGNLVVPGVPYPSGGGLKGGLQWV
                     KKEGTESVNTAFDAKAEKNKDTYIHPDNSRFPNDVAKRHSTMIRRVDDAVADLIRLLK
                     DLKIDDNTMIVFSSDNGPHNEGGADSRHWHGAQNPQFFKSYGMMDGIKRDCWEGGMRV
                     PTLVRWPARIPKGQISLHPGQFHDWLATLADVAGVPVPARSDGVSLLPTLTGHADQQK
                     PGIVYAEYNFAGKTPEYKDFLGEHKGAQRGQQQIVFVDGLKGLRMGVKDADKDFMIFD
                     TLNDPQESKDLASSKPELQARMKAAALSNRRASLPSKTVFDSALVPAVDVKGTVSPGL
                     RWALYEGDFPWVPDFRQWKKPASAHGVTPSPSVKMNGSDKRGVELTGYVKIPEDGAYT
                     FYLTTDENKGSKAFVRLHGMELIDADKTYEPGAEVSSDLGDRKNPVYLKAGLHPIRIG
                     YVGNSGTASKLVLKWEGPGLSKKEIPASAFSHGKESR"
     gene            10225..10857
                     /locus_tag="PK077_01460"
     CDS             10225..10857
                     /locus_tag="PK077_01460"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRN23002.1"
                     /translation="MSDLYEYQAIDGSSKGPVDLVLLLRARNLGRLSNRTLVRKVPGG
                     DWRPLASFIPSMTVMDDDGEPSVLPDPGSEPGGADSGWSRKKYWIRVIKAWCRFEGRA
                     GKGELHEVFSRVGVAWLAVAGVPSFAECGFFPSLVPWVELVSPLLYVVLAVLSIPLAA
                     TMVRRLHDVGRSGFFLVFLAVPFIGWLLLWYLSHGESRIGENKYGDPPWN"
     gene            complement(10889..11854)
                     /locus_tag="PK077_01465"
     CDS             complement(10889..11854)
                     /locus_tag="PK077_01465"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23003.1"
                     /translation="MIFHHVFGLYALPAGVDTAWIAPQLTKAAPIFKICVPIFIFITG
                     YAMGWKTNSSPTFGSLIKTGFSHYFKFWKIYFLCLLLAILVSWAFPLPILPSVADMGW
                     KNGLLVVTGLKPCYPDWWYMALFAAATMALYPICAWITHHIAPVPSMAALLGVSLLFQ
                     STAHIPSLPGIAYSFPPFLPCFILGYMCAFLASRLSALSISQSLGAILLLALEILSIH
                     LFSFSKAKTLTVIFLFTLWCLPWITRKLRLTPLLTLLGTYSALMWLNHRFIFGYHFSW
                     DLYGTQSISVVFIVTLVSSLLLAMAMQKLFNRMIPSNPQTQSKTL"
     gene            11840..12121
                     /locus_tag="PK077_01470"
     CDS             11840..12121
                     /locus_tag="PK077_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23004.1"
                     /translation="MMKDHEENSIPFEDSGIFPGKRKMLLKARNSKGREKAHLRRSST
                     DFQSERANISRKQLSTKAVWIEKEKRHISPTPLFLPFHFDDPFVLKIEC"
     gene            complement(12210..15056)
                     /gene="gcvP"
                     /locus_tag="PK077_01475"
     CDS             complement(12210..15056)
                     /gene="gcvP"
                     /locus_tag="PK077_01475"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HRN23005.1"
                     /translation="MNTHSDFASRHIGPQGEERREMLDSLGYRTLDELIADIVPADIR
                     MKAPLDLPAAKSETEALEELRSILRKNKLLKTFIGQGYYGTITPSVILRNVLENPGWY
                     TAYTPYQPEIAQGRLEMLMNFQTMVSSLTGLPVANASLLDEGTAAAEAVTMCRNARPK
                     ANTFFVADTCHPQTISVIRTRAAFQGVNIIVGDCSSFDPASIGADLAGVLVQYPDTLG
                     RICDYTDFFSRVHATGALCVVAADLMALTVIREPGAFGADICIGNTQRFGIPMGFGGP
                     HAAYMSCTDALKRRMPGRLIGMSIDTLGRPAYRLALQTREQHIRRDKATSNICTAQVL
                     LAVLAAFYAVYHGQEGLKRIGTEIHLKTKSLYKALTEAGIAIKNKNFFDTLLLSVPGQ
                     ADAMVQKALEAGYNIRRVDADHAAISLDETATCADVAALASALAGAETSAACDCDAPA
                     WDPVHTRQTPFCTEKAFNSYHSETEMMRYIRRLESRDLALNEAMIPLGSCTMKLNAAS
                     EMIPITWPEANSLHPFVPADQSEGIREMLSVLSDRLAKITGFAAVSLQPNAGAAGEYA
                     GLLAIRRYQKHAGEGHRNVCLIPTSAHGTNPASSAMAGLKVVPVKCDERGNIDMADLK
                     SQAEAHKDNLSCIMVTYPSTHGVYEQTIKELCDIVHANGGQVYMDGANMNAQVGLTCP
                     GCIGADVCHLNLHKTFAMPHGGGGPGIGPIGVAEHLVPFLPGHLTLGHEEGAVASAAW
                     GSASIAAICWMYLSMMGPDGLREATEMAILNANYIAKKLGHLFPVLYSGNKGLVAHEC
                     ILDPRQLTHDAGLTVDDIAKRLMDYGFHGPTMSFPVPGTLMVEPTESEPKKELDRFIE
                     AMERIHAEITAIINGTADKEDNVLKNSPHTAEMVSADEWRHPYSRSEAAYPVSGLLIH
                     KFWPYVGRVDNVYGDRNLVCTCDTVEEFSKAVEL"
     gene            complement(15152..15532)
                     /gene="gcvH"
                     /locus_tag="PK077_01480"
     CDS             complement(15152..15532)
                     /gene="gcvH"
                     /locus_tag="PK077_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRN23006.1"
                     /translation="MHDVPENLLYSKDHEWIEIDGDIGTIGISDHAQAELSDVVFVDL
                     PEVGATVAAGDPVAVVESVKAASDVYTPVSGEILEVNEELSNDPSLINSDPYGAGWLY
                     KIRLDVPTETEDMMNATDYEEYCS"
     gene            complement(15603..16688)
                     /gene="gcvT"
                     /locus_tag="PK077_01485"
     CDS             complement(15603..16688)
                     /gene="gcvT"
                     /locus_tag="PK077_01485"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRN23007.1"
                     /translation="MTDTDVKSTPLAAKHVELGARMVPFAGWNMPVQYTGILDEHKAV
                     RETCGIFDISHMGQFTVAGGSAAAWLNSMLTNDINKLNVGQGQYSVMLNDRAGVIDDL
                     ILYRMEPETFFVVVNASKIDEDFAWLSAHQPAGVTLENHSDEYVGLAVQGPECGEVFS
                     HVIPGVELPPRNGISRITAEGTDLIVCRTGYTGEDGFEFFCPAKEGVKWFEAFLGAGA
                     KPCGLGARDSLRLEMCYPLNGSDLSPDKTPLEAGLGFFCALDTDFIGSDILREQKANG
                     LSRRLAAIEYTGKGAPPRAHYAVHVPGGEAIGELTSGVLSPSLMKGIALAYLPAAYAK
                     VGTELEIDVRGRKFPAVVVKKPFYKKG"
     gene            complement(16764..>17388)
                     /locus_tag="PK077_01490"
     CDS             complement(16764..>17388)
                     /locus_tag="PK077_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HRN23008.1"
                     /translation="KLLPHLNEGNPALTLEPQLDDDERKLLHSFQLLSDKKSIFACNV
                     NEDELADAISNPDAHPYVSQVKKYVAEHHNAEAIVISARIEEELIDVSEEESREFLES
                     LGVRDSGVSDLIRAVYHLLGLRTYLTTGIKETRAWTIPAGAKAPQAAGVIHTDFERGF
                     IAAEVVHYDDLVSCGGKAGAREHGKLRIEGKEYVVKDGDVIEFRFNV"
BASE COUNT         3553 a         4333 c         5483 g         4019 t
ORIGIN      
        1 tgaagttgcc cagaaccatc agggtgcggg tcggccagtt ggtgcggcgc aggtaggggt
       61 ggcaggaacc ttcgtcaaag aggccggagg tgccgtagcg gagatgcccc atcaggattt
      121 ccccgccgta gtcgaagttg tttttgatgt ctgccgcatc cttcaggttg accaggccgc
      181 ggcgaacttt tttattgagc ttcttgattt gcccgttgaa gatgctcgta agggcgtctt
      241 tgctggcgtc ccgggtgcgg aacagataag gctgccccag gggcatgttc agcttgacgc
      301 agcctatgcc cgcgccgtcc tgtccgcggt tgtactgctt ttccatcagg atgagcagtt
      361 tgttgaacgc ccagagggtg gagccgtatt tatcctgaaa ataagagaga ggcttaagca
      421 gacgaatggc ggctacgccg cactcgtgtt taagaaaatc gctcatggga tgatgatgaa
      481 aagattgtcc tgtaagtgtg gcgtatccgt tgcaaggaga tggtgttttt attcgccctg
      541 tactttgaca tgaatgccgc ggccttctct cagcgtgccg atgaccgtgc cgcccggacc
      601 tgccttcaat gcggcttccg catcttcagg tctcacgata gctacccagc caatgcccat
      661 gttaaaggtg ttgaagcggt cctgcggatc cacatgcttc aggattttct gggcggcggt
      721 gttgtcccaa tagggaatgg agaggtctgc gccgtagtcg cccaggaagc gttccaggtt
      781 ttccgggagg cctccccccg tgatatgggc gtatgccttc ggggtgacat tgaagcggcg
      841 catatctgcc accacgtcat ggtacaagcg ggtgggctga agaagggaac ggagttcctc
      901 ttcatcaact acatccggat tttcttccag aatgcggcgg atgaggctcc agccgttggc
      961 atggaagctg tcggatttgt agccgatgaa tacgtctccg ggccggacgg ttttcggatc
     1021 aatcagtttg cttttttcac aacagccgat gcagaagccg gagagttcca cgatggattc
     1081 cggcaccaca ccgggcattt ccgccgtttc ccctccggcc aggatgcagt tgcaggattc
     1141 caggtagtcg cacatgccgg ccaccaggcg ggtgatgcgg gagcgttccc gttccagatt
     1201 ggagatgccc agataatcca ggaaaaggag cgggtcgccg cccgtggtaa gaatgtcgtt
     1261 gacgttcatg gccaccaggt ctttgccggc ggtttccacc atgtccagtt caaagagaat
     1321 ttccgtcttg gtgcctacgc cgtcgcaccc ggtgacgatg accggttcct tataggaact
     1381 caaatcatag gcagcggcga aaaggccaaa ggcctggtga aggtttttgc gttcttttaa
     1441 cgtgagagct gatgtcggat acaaaagcgg ctgcttcttt ggtatcgacc cccgattgct
     1501 tgtaggttag tttgccggac attgaacgga tgaactgggt gtaccagcat cctagaggaa
     1561 ggctccctcc gcggcgagtg aaaaaagaag tatttttgcc atagaccgcg cgggaggagc
     1621 tgttttcatt ccacttctat cttgcagtcg cgccggagcc accaggcatc cagggagaat
     1681 ttgcccggtc ccgtatagaa gatggtcaga tagatcagca ggtagatggc cgcaagttcc
     1741 tgcgctcccc agccggaaag cccaaggaca agaaagagcg ctacacacat gttaataatc
     1801 aggataaggg cggccagacg ggtgaggaat cccagcaaga ggaggacgga acatccgaat
     1861 tccgcgccta cgcacagcag cagggacaga aaattgccga ttcccagagg atcctggaac
     1921 gccgcccagt ttccgctgat aagcatgccc agtttgggaa tgccatgcac aagcatcatg
     1981 actgctacgc ccaggcgcag caacagaacg ccgaagtcca tactggtgtt ggtggccagg
     2041 gggccaagca ggcggcatgc ggatgatttg cttttattct gttccatgca ctgtagaccg
     2101 ttttggagac agaaatgctc aattaaaaca ggatagaaat gagtttccgg gatagcgggg
     2161 aaactgcctg tgacgcagtc atgccgcctg ccgcgggtgt gtgcgggcgg aatagcgtgt
     2221 gccgggctta tgggaacatg caggagttcc gcatgttccc ataagcggtt ctggaagttt
     2281 ttaatatttt ttcgcaggaa tgaggcggaa agctgcgggg gattgggagg acttgatttg
     2341 caggggcttg gtcgggtctt tttgagtttc cgggccaatg gcgatgataa gcctgtcctt
     2401 gggatgaatg acggtggagt tttcaaagat gagcgtattg gttccgtccg tcagtttcca
     2461 tccatccaaa aggatgccgg agttgcctgg gttgttgata atcacgttgc cggtgcccgg
     2521 ttccaatctg ggggcgggct gcttaagcct gaggcttttt gccagcttgg caaaggtgat
     2581 ttccgcaatg cgccgtgcgc cctgttccgt agggtgcaac ccgtcaacgt accattccgg
     2641 atggtcggag agcgggtgca gcgcgtcaaa gagactgact ttgtacttgc gcgctacggc
     2701 ggcaatcagt ttttctgctt ccgggcggtt ggcggatgag ccgttgtccg agcctgcata
     2761 tttacgtacg tccggatagc agttgccggg aaatgccttc ttgttgagcg ggccgcggta
     2821 gtccggcccc agcagccccc aggcaaagaa ccgtgttttg ggattggcgt ttttccacgc
     2881 atgaagcaga gcgtcatagc ctttggagaa cagttcgggg ttccaccagc ggcccgtgtc
     2941 gttgatgcca aggttgcaga tataaatatc cgccttgaat tccagggctt gcttgtgttc
     3001 cctggtgctg ccgtaccagc gtcctgcctg gcccgggtag ttgcctgccg tttttccggg
     3061 attgccgaag cctctgacgt caaagtccgg ccccatgagt gtggcgagca cttgcggata
     3121 ccgggtttct ccgggtttaa ggccggtgcc gaaggtgata ctgtccccca cgcaggctac
     3181 tttggcggga gcagcttgtg ccgcaatgcc cagtgagagt gtggctgtca gagaagcgag
     3241 gataagatga aggttcatgg agtgatggtg tggtaaaagt tattttttca gaacaggttc
     3301 gtccgtccgg aagggacggg cggggagctg gttgccgttg accaggttgg gggtcacgaa
     3361 acggttccag caataccggg cgtgtttggg ggattttact tccggtgatg agaggcggat
     3421 aacggctgtg ttgccttttc tgtttatgat ttccgctgcg gcgggatgat aagttccgtc
     3481 cgtcccggca atttcaaact gggccggttc tttgccgtcc gtggtggtca gtccggcagc
     3541 ttgatccagt tgaaccagga tgttggaacc ggaaggtttg aaggccttga gggcagggcc
     3601 ttcacagggg atctttttac cgtaaacctt gttcagggca gtatttcctg cacgttcccc
     3661 cacagggcgc ttgaatggag gatgaacgtc ggaattggtg gagccaagat caatggtgtt
     3721 gacgctgtaa acttttggaa cggttttggc cacggcgtcc tgcatttcac ggaattccgg
     3781 ccatccggca cggatttttg acgggtcgtt gatgcggggg agctggatca tgacgaaagg
     3841 catttccggg ttacggaaag cttttctcca ggaggagatc atggttttca gcagcatgct
     3901 gttttgggca ttgtcaatga tctccgcatc ggattcgccc tgataccaga tgacgcccgt
     3961 gatggggagg gccgttgtcc atgcgatgcc ggattcgtaa aggaacgccg gcttgtaggg
     4021 atggtccgga gagccctggt tcagacgcgg agagatgttt ttcctggccc gtcccctcac
     4081 ccagtcggag ataagggggg agtccagcca gtggttgccg cgcagggtgc ggaagctgtt
     4141 gttggcatta atgacctgcc gggggatcca cgctgcgatt tccgagcctc ccagggagga
     4201 gtggatgatg cctaccggaa tgcccagccc ttcgcggagt tttttcccga agtagtagcc
     4261 cacggcagtc attggccctg aggtggcggg agagctggca gcccattgcc ctttatagaa
     4321 gttgtccgtg gtgacggcgt caaaatcttt cccggagtag ggggcgttgt tggtgtgggc
     4381 ttgcggaacg ttgttgagca gacggagctg gtcgtctccg gaagccgcta tgtcttcttt
     4441 ggccgtcgtg gtctggttca gacggaacag catgttggat tggccggaag ccagccatac
     4501 ttctcccacg agcacatctt ccaatttggc ggagtccccg ttttgcacgg ccgccagcgt
     4561 ctggcctgtg ccgcagggag gcatgggagg cagcgtgacg ctccattttc ctttggaatc
     4621 ggtttttgtt ttcagtgcag tgtttccaaa agtgacggtt atttccctgt tgggggcggc
     4681 cgtgccggaa acgggaatgt ttttcccgtg gggaagtacc atatgggaac cgaaaatccg
     4741 gtcgaaccgg ggggcgtccg cccacgctgc gttaagggcg atggcggcag ctaggatgca
     4801 tgaagtatgg aggcgcatgg tttgaggatt atttgagaag gagttcgttc cgtgagaggc
     4861 cggtcagcct gccgagccgt ttaatcaggc gggtcatgat gaagacaaga gctgccagaa
     4921 tggggattcc gatgatcagg aagccccacc aggtgatgct gccgatagcg gcattgtagg
     4981 ccacttgctg ttccgcttcc ggctggcgta tgacagagga catgaaagag aggctcagga
     5041 agaaattgcc gatggaggag gcaaccagcg tgcccgccag aatccaggac gagtttgcca
     5101 gcagtttctg ataagcccgt tcctcgccgt tggcggccac cgtttgttct atcctgcgca
     5161 cgtcgaataa ctccggcgtg tagatgaaca tgttgaggag gggggtgccg gtggagcggg
     5221 agacgacgac ggctgccgcc aggatgaggg ggatcagggc ttcttttccg gcgaacagcc
     5281 agggtgtggc gctgtggatg cgcccttccg gcccgatgac gaagatgcta atgacgcccg
     5341 tcagcagcac gccggccatg cctacgccgg acatcaggtc aaatttcctg gagacgacca
     5401 gggaacggat accatagaca agggggaggg aaagggcgat gacgagggcc cataccgggc
     5461 cgatatgcca gaaattttct ccttccgggc gttccagggg attggcgggc cccgcgctgc
     5521 aatagtccag aatcaacacc gggaggagga tgttgatgaa aatccccagc agggaggact
     5581 gtgatttgtt tggcgtttta tccatgaatt cggagggaaa tatttgtacc atatgcgaat
     5641 gcgcccctgc aagttgcaac cccgtgtgtg acagtaaaga ccagaagcgg ggagagccgt
     5701 ttttttattc gcaggcggct ttgcggaaat cgtggcatct acggtatctt ccgttctttt
     5761 ggctggacac gggagggaat aatgttcagc ttctcccgtg actttgacag atcatttttc
     5821 cccatgactc aaacatcttc tttttcggct cttggtattt caccggagat tctggaggct
     5881 atcgaagtgc tcggcttcga tactccttcc tccattcagg agcaggcgat tcccgccgcg
     5941 atcggagggg ccgatattgt ggggttgtcc catacgggct ccgggaagac gcttgccttt
     6001 gccattccgg ccctggagtg cattgagcct gaggaacgct gcgttcaggt gctggccctc
     6061 tgtcctactc gggaactggc cgttcagatt tgccgggagg tggacaagct ggctttgttc
     6121 atggacggtg tttccgccgt ccccatttac ggaggctctt ctttccgtcc gcaactggac
     6181 gccctgaggc gcggggtaca gtttgtggtg ggaacgcccg gacgcgtcat ggacctgatg
     6241 gagtccgggg cgctaaggct ggacggtctc cgcatgttga ttctggatga agcggacgag
     6301 atgctggata tggggtttct ggaagatatc gaacgtatcg cggacgctat gccggaagcg
     6361 aagcagaccc tgtttttctc cgctaccatg tctccggaaa tcagccgcct ggtgagcagg
     6421 ttcatgaagg agcccgtcca gatttccatt gaacggccta cgctgacggt gcctaccatt
     6481 gaacaggttt attacgaagt ggtgttttcc tcccggattg aggtactcag ccggctgctt
     6541 gacaccggaa agattaaaat ggggctggtg ttcgccaata ccaaaaaggt ggtggatgac
     6601 gtggtggacg gtctgacggc ccgcggttat ccggtggatc gccttcatgg ggatatgccc
     6661 cagatgatga gggagcgcgt gatggattcc ttccgcaagg ggagcctgcg ccttctggtg
     6721 gcgacggata ttgccgctcg cggcctggat gtggacgatg tggatgccgt ggtgaatttt
     6781 gaattgccgc gtgatccgga ggattacgtg caccgcatcg gccgcacggc acgcgccggc
     6841 cgcaagggga aggccatcac ttttgtgggg cgccgtgatt tttccctgat gagccgcatt
     6901 gaacgtttca tcggcgttaa gttgactccg gaacctgtgt tgactgccgt tcaggtggac
     6961 cagctccgga cagagtccct ggtggatgat atcctggaac gtctcaagcc ggatgccgtg
     7021 ctgccggagg aattgaagga agtggaggct gcgccggagg ccatggctgc ggccctgttt
     7081 gacttgctcc gggaacgtac gcaccgggaa gtgcagccca ttccggaaga caagcccgcc
     7141 cgcagggcgc ggcgtatgcc gcagaccggg gaggaggcgg agagcccgca gaagggtgat
     7201 tcctggcagc ataaggggga tacggttacg ctgtttatga atggaggccg gatgattggc
     7261 ttgcggccca aagacatagc cgggttattt tacaatacgg tggaaatgga aaagggagcc
     7321 gtgggcgata tccggatttt ccccaagcat tccctgatag aagtggacgc ttccgtggcg
     7381 cccctgcttc tggaggccct ggcgacggct accgtctgcg gctatgaagt taatgtccgt
     7441 gaagacaagg gggctccgga atattccgga actccgcgct cttcccgccg caatggagga
     7501 ttccgcaaca gttaccgcgg agagcgggac cgcagtgacc ggagcggatt caggggaaac
     7561 cgcgggggct tccgcagtga ccgcggcgaa cgctggggcg aacgttcccg cagggacgac
     7621 aggaagaagc gtttctgatg ccttcgctat tccggacagg gttattaacg ctgttccgga
     7681 attggcgcgg acattttgga acgttccggt tcatgattgc tgtttccgcc ggtttctgta
     7741 ttttctgccc ggcgggggga ttgccgtttt attcaggata aagcatggcc gggcggggct
     7801 ggtgaccggg gcgccgggaa tgcaggagga attttctccc attcctggct tcagccagaa
     7861 aagccgctca ttggtgcagt atggcgggtt gtagttgttc cggctgtgaa ggctggttcc
     7921 ctgatgtttt ttgccgataa tactggcgat tcaatcctct gtgccctttc ctcgtaattt
     7981 tcttgaggga tgagaaaatt catgtaagat aacgctttat tttaacgtct tttttagagt
     8041 ctattttctt tgaatcatga agctggtatc tttactatcc gttcttctga cttccctggt
     8101 tccgtgcatg ggtgcttccg ggcaggcaac gaagccggct gcccggtctg tcaaaacttc
     8161 aaaacccaat gttattttca ttctggtgga cgacatgggc tggggtgatc tggattccaa
     8221 ctggagccag cagaagctga atggccggac ggtggagaga aagaacgagt tcaagactcc
     8281 tgccctgtcc gctctggctc gggaggggat tcagctgcgc cgtcattatt ccgccgctcc
     8341 ggtttgcgct ccggcgcgcg cctccctgct gctgggggtg caccagggga attcccgcgt
     8401 ggtgagaaac aaccgtttcg accagcctat tgaagattcc catacgctgg gtaccgtgat
     8461 gcgcgatgcc gggtatgata ctgctgccat tggcaaatgg ggtgtaggcg gcggaggcca
     8521 gagccatgtg cccatgaccg ccggccccca tcagcgggga ttcaattatt tttacggtat
     8581 cctggaccat ttggcagggc attttcacta tccttccgaa tcccgtgacg ttttcgaata
     8641 caacggttac gcttccaatc ccgagtggaa aaatatcaag gaccaggtgc cccagacggc
     8701 ttattccacg gatttatttg ccgcccgcgc caagcagtgg attgtggacc agcgcaagtc
     8761 cgcccgcaag accggaaagc cgtttttcct gtacctggct tttccggcgc cccacggcaa
     8821 tctggtggtt ccgggggttc cctatccttc cggcggcgga ttgaaaggag gcctgcaatg
     8881 ggtaaagaag gaaggcacgg aatccgtgaa tacggctttt gacgccaagg cggaaaagaa
     8941 taaggatacc tatattcatc cggacaattc ccgtttcccg aatgatgtgg ccaaacgtca
     9001 ttccaccatg atccgccgcg tggacgatgc cgtggctgat ttgatccgtc tgctgaaaga
     9061 tttgaagatt gacgataata cgatgatcgt tttctcgtcc gacaatggcc cccacaatga
     9121 gggtggcgcc gactccaggc actggcacgg agcgcaaaat ccgcagtttt tcaagagtta
     9181 tggcatgatg gacggtatta agcgcgattg ctgggaagga ggcatgcgcg tgcctacgct
     9241 ggtacgctgg cccgcccgca ttcccaaagg gcagatcagc ctgcatcctg gacagttcca
     9301 tgactggctg gctacgctgg cggatgtcgc cggggtgccg gttccagccc gcagtgacgg
     9361 cgtttccctg ctgccgacgc tgaccggtca tgcggaccag cagaagcccg gcattgttta
     9421 tgccgaatat aatttcgccg gcaaaacgcc ggagtataag gatttcctgg gcgaacacaa
     9481 gggagcgcag cgaggccagc agcagattgt ttttgtggat ggattgaaag gcttgcgcat
     9541 gggggtgaag gatgcggaca aggatttcat gatttttgat actttgaatg atccgcagga
     9601 aagcaaagat ctcgcatcct ccaagccgga acttcaggcc cgcatgaaag ccgccgcttt
     9661 gtccaatcgc cgggcttccc ttccttccaa aacagtgttt gattctgcgc tggtgcctgc
     9721 cgtggatgtg aagggaaccg tttctcccgg gttgcgatgg gccttgtatg agggggattt
     9781 tccctgggta ccggatttcc gccagtggaa gaagcctgct tccgcccatg gcgtgacgcc
     9841 ttccccatcc gtgaaaatga acggttcgga caagcgcggt gtggagctga cgggatatgt
     9901 gaagattcca gaggacggag catatacgtt ttatctgacc acggatgaaa ataagggcag
     9961 caaggctttt gtccgtctgc acggcatgga attgattgac gcggacaaaa cttatgagcc
    10021 gggggccgag gtctcctccg atctgggtga ccggaagaat cccgtttatt tgaaagctgg
    10081 acttcatccc atccgcattg gctatgtggg gaactccggt actgcctcca aactggtttt
    10141 gaagtgggaa ggtcccggtt tgtccaagaa ggagattccc gcctccgcct tcagccacgg
    10201 gaaggaatcc aggtaagcgt ccccatgagc gatctgtacg agtatcaggc aattgacggt
    10261 tcttccaaag gcccggttga cctggtgctt ttgctgcggg cccgtaacct cggccgcctg
    10321 tccaaccgga ctttggtccg gaaggttccc ggcggagact ggaggcctct ggcctctttt
    10381 atcccctcca tgactgttat ggatgatgat ggggagccct ccgttctgcc ggatcccggt
    10441 tcagagcctg gaggcgccga ctccggctgg agcaggaaga agtactggat acgcgtcatc
    10501 aaggcatggt gccgttttga gggccgtgcc gggaaagggg agctgcacga ggttttttcc
    10561 agagtggggg ttgcgtggct ggccgtggcg ggagtcccct cttttgcgga atgcggtttt
    10621 ttcccgtccc tggttccgtg ggttgagctg gtttcccctc tgctgtacgt ggtgctcgcc
    10681 gtgctgtcta ttccgctggc ggctaccatg gtccggcgtc tgcatgatgt cgggaggagc
    10741 ggctttttcc tggtttttct ggccgttccc ttcatagggt ggctgctgtt gtggtattta
    10801 tcccatgggg aaagccggat tggagaaaat aagtacggag atcccccttg gaattaagag
    10861 gtgttggaat ccggaccttg taaagttgct acaaagtttt tgactgcgtt tgcggatttg
    10921 agggaatcat ccggttaaaa agtttttgca tggccatggc cagcagaagg gaggagacca
    10981 gcgtcacgat gaatactacg ctgatgcttt gggttccata gaggtcccag ctgaagtggt
    11041 agccgaaaat gaatctgtga ttcagccaca taagtgcgga ataagttccc agcagcgtca
    11101 agagcggtgt gaggcggagt ttccgggtta tccaaggcag acaccagagg gtgaagagaa
    11161 agataaccgt cagggttttg gcttttgaaa aactgaagag atggatggat agtatttcaa
    11221 gagccagcaa caggattgct cccaagcttt gggaaattga aagagcagag aggcgtgaag
    11281 ccagaaaggc gcacatgtag ccgagaatga agcagggcag gaaaggaggg aaggaatagg
    11341 cgattcccgg caggctggga atgtgggccg tactttgaaa taaaagggac actcccagca
    11401 gagctgccat ggacgggaca ggcgcaatgt gatgcgtgat ccacgcacag attggataaa
    11461 gggccatggt ggctgccgcg aaaagagcca tgtaccacca gtcgggataa cagggtttca
    11521 atcctgttac gacaagcagg ccatttttcc agcccatgtc ggcaacggat ggcagaatgg
    11581 gcaaggggaa tgcccaggaa accagaatag ccagaaggag gcataagaaa taaattttcc
    11641 agaatttaaa gtaatgggaa aagcctgttt taatgaggga accgaaggtt ggggatgaat
    11701 ttgttttcca tcccatagcg taccccgtga tgaaaataaa aatgggcacg caaattttaa
    11761 agatgggagc agctttggta agctggggag caatccatgc cgtatccaca cctgcgggta
    11821 gagcgtataa gccgaagaca tgatgaaaga tcatgaagag aattccatac cctttgagga
    11881 tagcggaatc ttccctggaa aaagaaagat gttgttgaag gccaggaaca gtaagggcag
    11941 agaaaaagct catctgagac gtagctcaac ggatttccaa tccgaaagag caaacatatc
    12001 cagaaaacaa ttaagcacaa aagcagtatg gattgaaaaa gaaaaaaggc acatttctcc
    12061 caccccgctt ttcctcccct ttcattttga tgacccattt gttctcaaaa tagagtgttg
    12121 acttcggatt ggaaatccga ttcttgaaca taagaaaaag gctgctccaa atgaatggaa
    12181 cagccttttt gaaaggaagg atgagatttt tataactcta cggccttgga gaattcctcc
    12241 accgtgtcgc aggtgcagac cagattgcgg tctccgtaca cattatccac ccggcctaca
    12301 tacggccaga atttgtgaat aaggagaccg gagaccggat aagccgcttc actgcgggag
    12361 taggggtgcc gccattcgtc ggcggagacc atttctgcgg tatggggcga gtttttcagg
    12421 acgttgtctt ccttgtctgc cgtaccgttg ataatggccg tgatttccgc atgaatgcgt
    12481 tccatggctt caatgaagcg gtccagttct tttttgggtt ccgattccgt cggctccacc
    12541 atcagggtgc ccggaacggg gaaggacatg gtggggccgt ggaagccgta gtccatgagc
    12601 cttttggcaa tgtcatccac cgtgaggccg gcgtcatgag tgagctgacg cggatccaga
    12661 atgcattcat gcgccaccag ccccttgttg ccggagtaga gaaccgggaa gagatgtccg
    12721 agtttcttgg caatgtagtt ggcgttaagg atggccattt ccgtggcttc cctgaggccg
    12781 tccggcccca tcatggagag gtacatccag cagatggcgg cgatggaggc gcttccccac
    12841 gccgcagacg ctacggcccc ttcttcatgg cccagcgtga ggtgcccggg caggaagggt
    12901 acgaggtgtt ccgctacgcc gatgggcccg atgccgggac cgccgccgcc gtgcggcatg
    12961 gcaaaggttt tatgcaggtt caggtggcag acgtccgcgc caatgcagcc ggggcaagtc
    13021 agacctacct gggcattcat gttggcgccg tccatgtaca cctggccgcc attggcatgg
    13081 acgatgtcgc agagttcctt gattgtctgt tcatataccc cgtgcgtgga ggggtacgtg
    13141 accatgatgc aggagagatt gtccttatgg gcttccgcct gggatttcag gtctgccatg
    13201 tcgatgttgc ctctttcgtc gcatttgacg ggcaccacct tgaggccggc catggcggag
    13261 gatgcgggat ttgttccgtg ggcggaggtg ggaatgaggc agacattgcg gtggccttct
    13321 ccggcgtgct tctggtagcg gcggatggcc aacagtccgg catattcccc tgcggcgccg
    13381 gcgttgggct ggagggatac ggcggcaaag ccggtgattt tcgccagacg gtcggacagg
    13441 acggagagca tttcccggat gccttccgac tggtccgccg gaacaaaggg atgcagggag
    13501 ttggcttccg gccacgtgat gggaatcatt tcagaggcgg cgttcagctt catcgtgcag
    13561 gagcccagag ggatcatggc ttcatttaga gccaggtccc gggattccag gcggcggatg
    13621 tagcgcatca tttccgtttc ggaatggtag gagttgaaag ccttttccgt gcagaaaggc
    13681 gtctggcgcg tgtggacggg atcccaggca ggagcgtcgc aatcgcaggc agcggatgtt
    13741 tccgcacctg caagggcgga ggccagtgcc gctacatccg cacaggttgc ggtttcatcc
    13801 agagagatgg ctgcgtggtc ggcatccacc ctgcggatgt tgtacccggc ttccagggct
    13861 ttttgcacca tggcgtccgc ttgtccggga acggacagca gcagggtgtc aaagaagttt
    13921 ttgtttttaa tggcgatgcc ggcttccgtg agcgccttgt acaggctttt ggttttcaag
    13981 tggatttccg tgccgatgcg tttgaggcct tcctgtccgt ggtatacggc gtaaaaggcg
    14041 gcaagcacgg ccaggagcac ctgggcggtg cagatgttgg aggtcgcctt gtcgcggcga
    14101 atatgctgtt ccctcgtttg cagggccagg cggtaggccg ggcggcccag ggtatctatg
    14161 gacatgccga taaggcggcc gggcatgcgg cgtttgagag cgtccgtgca ggacatgtag
    14221 gcggcatggg ggccgccgaa gcccattggg atgccgaagc gctgcgtgtt gccgatgcag
    14281 atatcggctc cgaaggcgcc gggttcccgg atgacggtaa gggccatgag atccgccgcc
    14341 acgacgcaga gcgcgcccgt ggcatgcacg cgggaaaaga agtccgtgta gtcgcagatg
    14401 cggccgagcg tgtccggata ttggacaagc actccggcca gatccgcacc gatggatgcg
    14461 gggtcaaagg atgagcaatc tcccacgatg atgttaacgc cctggaaggc ggcgcgggta
    14521 cggatgacgc tgatggtctg gggatggcag gtgtccgcca caaagaaggt gtttgctttg
    14581 gggcgcgcgt tccggcacat ggtgacggct tctgctgctg cggtgccttc atccagcagg
    14641 gaggcgttcg ctaccggcag ccctgtcagg gaagagacca tggtctggaa attcatgagc
    14701 atttccagcc gtccctgggc gatttccggt tgataagggg tgtaggccgt gtaccagccg
    14761 gggttctcca gtacattgcg gaggatgacg gagggagtga tggtgccgta atagccctgg
    14821 ccaatgaagg ttttcaggag cttgtttttc cgcagaatgg aacggagttc ctccagggct
    14881 tccgtttccg atttggctgc gggcaggtcc agcggggcct tcatgcggat atctgcgggg
    14941 acgatgtccg caatgagttc gtcaagcgtc cggtagccga ggctgtcaag catttcccgg
    15001 cgttcttccc cctgggggcc gatgtgccgg ctggcaaaat ctgagtgggt attcatgtgg
    15061 tatctctggc tggtaatagc gtccggccgt ggaacgggcc aaacaaaatg ccccgggatg
    15121 gaatcaccgg ggcgggggag ggaagaatgg ttcaggagca gtattcctcg taatccgtgg
    15181 cgttcatcat atcttccgtt tccgtgggaa cgtccaggcg gattttgtac agccagcctg
    15241 cgccgtaggg gtcggagtta atgagggagg ggtcattgga aagctcttcg ttcacttcca
    15301 ggatttctcc tgaaacgggg gtgtacacgt cgctggcggc tttgacagat tccacgacag
    15361 cgacagggtc gccggccgcg acggtggcgc ctacttcggg aaggtctaca aagacgacat
    15421 cggagagttc ggcctgggca tggtcggaaa tgccgatggt gccgatgtcc ccgtcaattt
    15481 caatccattc gtgatccttg gaatacagca gattttctgg tacgtcgtgc ataagatgta
    15541 attgtttgaa gtgagagtgt agtaaaatga ggtgtttgtc agtttatttt tgcgccgtcg
    15601 tgtcagcctt ttttataaaa gggttttttt acgaccacgg ctgggaattt tcttcccctt
    15661 acgtcaattt ccagctccgt gccgaccttg gcataggcgg caggcaggta ggccagggca
    15721 atgcccttca tcagcgaagg agagagcacg ccgctggtaa gttctccgat ggcttctccg
    15781 ccggggacgt gcacggcata gtgggcgcgg ggaggagctc ccttgccggt gtattcaata
    15841 gcggccagcc gacggctgag gccgttggct ttttgttccc ggagaatatc ggaaccgatg
    15901 aagtccgtat ccagcgcgca gaagaatccg agcccggctt ccagaggcgt cttgtccgga
    15961 gagaggtcgg agccgttcag aggatagcac atttccagac gcaggctgtc gcgggcgccc
    16021 aggccgcagg gtttggctcc tgctccaaga aaggcttcaa accatttgac gccttcctta
    16081 gccgggcaga agaattcaaa accatcttcc ccggtatagc cggtgcggca gacaatgaga
    16141 tccgtccctt ccgccgtgat gcgggagatt ccattgcggg ggggaagttc cacaccgggg
    16201 atgacgtggg agaatacttc tccgcattcg ggaccttgaa cagccagccc cacgtattcg
    16261 tcggagtggt tttccagggt tacgcctgca ggttgatggg cggaaagcca tgcgaagtct
    16321 tcatcaatct tggaggcgtt caccactacg aagaaggttt ccggttccat ccggtacagg
    16381 ataaggtcgt caatgactcc ggccctgtca ttgagcatga cggagtattg cccctgccct
    16441 acattcagtt tgttgatgtc attggtcagc atggagttca gccaggccgc ggcggaaccg
    16501 ccggctaccg tgaactgccc catgtgggag atgtcaaaga tgccgcaggt ttcgcgcact
    16561 gctttgtgtt cgtccagaat gccggtgtac tgcaccggca tattccagcc ggcaaaagga
    16621 accattctgg ctcccagttc aacgtgttta gctgccaggg gggtggattt gacgtctgta
    16681 tcagtcatga ggcgatgtga aggttgtgag caatctgccc cttatatcag taaaagtcaa
    16741 tggaattccg gtaacgcttc cgtctagaca ttgaagcgga attcgatgac atccccgtct
    16801 ttgacgacat attcttttcc ttcgatgcgc agcttgccgt gttcccgcgc tccggccttg
    16861 ccgccgcagg aaaccaggtc gtcgtagtgc actacctccg cggcgataaa accgcgttcg
    16921 aagtcggtgt ggatgacgcc ggccgcctgg ggggcttttg ccccggccgg aatggtccag
    16981 gcccgcgttt ccttgattcc cgtggtgagg taggtgcgca ggcccagaag gtggtacacg
    17041 gcccggatga ggtcggaaac gccggaatcc cgcacgccca gggattccag aaattcgcgg
    17101 gattcttcct ccgagacgtc gatgagttcc tcttcaatcc gggcagagat gacaatggct
    17161 tccgcgttgt ggtgttccgc tacgtatttt ttcacctggg agacgtaggg atgggcgtcc
    17221 gggttggaga tggcgtccgc cagttcgtct tcattgacat tgcaggcgaa aatgcttttt
    17281 ttatccgaaa gcagctggaa ggaatggagc agcttgcgtt catcgtcgtc cagctgcggt
    17341 tcaagggtga gggcgggatt gccttcgttc aggtggggca ggagtttg
//
LOCUS       DAOMSU010000016        17335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_108_length_17335_cov_2.780671, whole genome shotgun sequence.
ACCESSION   DAOMSU010000016 DAOMSU010000000
VERSION     DAOMSU010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 17335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17335
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108_length_17335_cov_2.780671"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2456
                     /locus_tag="PK077_01495"
     CDS             <1..2456
                     /locus_tag="PK077_01495"
                     /inference="COORDINATES: protein motif:HMM:NF016945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HRN23009.1"
                     /translation="AEDVVARVTPSAKGAVLFHLNPDQGNKITISGIAGGIRVEASDV
                     RRLVAGYGWYLKNIAKVHFSWNGNRITLPSPLPVPASPVTVESPWNIVFAYNYCTLSY
                     TAAFWDWNRWQREIDFLALNGFTHALVTAGLEKTWEDFLTGLGYPREKALRFIPNPAF
                     AAWWNMGNLEGHGGPLSQQQINKMAQMGRRIVSRMEQLGMTPVLQGYVGFVPSDFQEN
                     VRIDGLKLIPQGEWVNFRRPWVVDPTCEAFPKLAADWYKALRKVYGIPGKMFGGDLFH
                     EGGRKGDIDVTQAAQAVQKAMQKASPGAFWVIQAWGGNPTRELLSGLDPERALVLQLT
                     KDMANGGKNLRTFNGIPWVWCELANFGGNTGMYGGVPLLSRLGSELSGYKDKGLVGMG
                     TLSEGLETNPLHYALFSDRLWTREDISVREWLGKYARQRYGFAPKAVVKALEVLSSSI
                     YNPVRSQEGCTESIICARPSWNVRKASTWSSGERYYHLGDIVKAARGYLKAANDQPNL
                     VKKETFRYDLVDVVRQSLAFYQLQQVRSAFDSGDLAAYRKQVKRFLSLISDMDALLAT
                     DSQFLLGTWQKRALDWGDSRQEKALMDKSAKMLITTWIDQVPRSLNDYSNRQWAGLVS
                     DFYLPRWKNFFEFQMDVLTGKKTRDAAHAAFMDKMVRDELAFAGNGKIYSAKPAGDTL
                     AVANRVMNTHREMLDALSAEEKHSSGSPWELQQGSPLQFDVTDQVTASGTYTATFQWK
                     NGPSALKIHSVRLYEGNREVASDVHEGRTGVENKDNIYRLELKKYRTNLDSYILKAEV
                     SGVSQGASKGEMVLKKLVD"
     gene            complement(2615..3034)
                     /locus_tag="PK077_01500"
     CDS             complement(2615..3034)
                     /locus_tag="PK077_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN23010.1"
                     /translation="MHINCIKLYFQPKDETHYFCYLHFLSLGTAQAATIFSTTNTNTA
                     NNYFGSATQSSSSVMSAATNRKEATLPETVYLNSLSLDMHSGCGTGTYKNNILNSWEG
                     MYLSAVNFSTSNEVVPEPATATLGLFGLSALLLRHRR"
     gene            complement(3455..5323)
                     /locus_tag="PK077_01505"
     CDS             complement(3455..5323)
                     /locus_tag="PK077_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HRN23011.1"
                     /translation="MASTSSQSSIPLPSCLEKPLPLDGFQGTPDEVEQQWYDQVYLGS
                     GDRMKQLTWRAVIVGMLLGSILSLTNLYANLKMGWSFGVALTAGIISFALWNAFVRLG
                     ISKSPMTILENTCMQSAASSAGYSTGGTLTSAVAALLLLTGQHMPLGTTFAWIFFIAV
                     LGVTMAIPMKRQMINIEQIRFPDSIATAETLKVLYSEGKKAAGQAKALLYSALFAAAN
                     AIAMAAGGERWLGTVQQHILGNWYQRTIFFKWDLMFVGAGALVGMKTSLSLFIGGTVC
                     WALYVPWLESQKLLPAGAGYRESVSWTLWGGTACMVVASIVAFLFQWKSIVRSFSSLG
                     VMFSLSKKRKLTDVEKIETPMSWFLTGQLISLGALGYLAHTSFNVPYWMSCIAVVISF
                     FLALVVCRITGEANITPTGAMGKVTQLIFGGIAPGHVTANLMAANITSGASSSSADLL
                     VDLKVGYLLGANPRKQFIAQFSGIFLGTLVSVLAFRSMVPDANALQAFNAPGARTWAA
                     TAEALGMGFSHLHSIKVLSIIAGGILGLILVLIPRYIPRTGKWLPTPIGFGLAWAIQW
                     NDSFLFFTGAVLGWAADHLFKAKSREYKVPTASGIIAGAALTGMAILMFSIYQAAL"
     gene            5444..6229
                     /gene="pyrF"
                     /locus_tag="PK077_01510"
     CDS             5444..6229
                     /gene="pyrF"
                     /locus_tag="PK077_01510"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HRN23012.1"
                     /translation="MSQSFTDKLAARIKETGSALCVGLDPRPGMDDLEAIPALLRKVV
                     EETAPYAAAFKPNIAYFEAMGLRGLEILEDLLPDMPKDVPVVLDAKRGDIGETQKYYA
                     HAYFERLGVDAVTLSPFMGYDTLEPFLNYEGKGVYLLAVTSNPGSADVERQELAGGRR
                     VFELVGDMVLRSVREGCKTSVGMVVGLTNADSSILERIPDAPLLIPGLGAQGGDLSCL
                     SGSGHVAPPLINVSRGIMYQNPELGFAEKAAGFAQRIREALNY"
     gene            6487..7125
                     /locus_tag="PK077_01515"
     CDS             6487..7125
                     /locus_tag="PK077_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HRN23013.1"
                     /translation="MWFPSVQGGEVEMTFVSPSEEVSQEMEEVQPTSVVPVAAPSTVS
                     PVPEIASDEDLLAMKLPENEDCSEMKDFLLDEAPSELLPEELAAMRISMNEVKRRQPV
                     KRPAVSLPGRPEPDQNISPDKKVRYKHAPSLPNSVNSSKVGKVNAVVKVVVGVNSHGE
                     PSSVNLLQSTGNAELDRLFMRWVRENWTFYPAEKDGVPIASKVVVPVRLNID"
     gene            7147..8838
                     /locus_tag="PK077_01520"
     CDS             7147..8838
                     /locus_tag="PK077_01520"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF011448.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HRN23014.1"
                     /translation="MLPEKTENRPVYVIGHQNPDTDAICSAIGNAEFLRTHGEPGAVA
                     ARCGEMTLRTSWVLEKAGVPEPILIHDVTPTAGTICRRDVISVSPDDTFLTAYRRMTE
                     NSLQTIPVIDADYNLHGLLRYFDLLSLLMPLNMTEMNVRSVFSSLSNIATTIDGKCLT
                     GESLSEEETQNILLVGASSEPSVRTRLANYRRKGIIRDLIVICGDRPNVQLYAVEHGV
                     RALVTTSGAFPSLDIIETAQTTGTCILSTPWDTASVGQLIRCSRKVREQVQKDYTVFP
                     ENMPLPELRQAAVKRKQALFPVMSIKTNKIIGVLSKTDLVDPPRTRVALVDHNEFSQA
                     VKGVEEAEIVEVMDHHRLGTQLSTRDPIRFLNEPVGSTSTLVARRFYHRNVEPSQAVA
                     VCLCAGILSDTLNLTSPTAARADREMLEWLTGIAKIDAKKFTEEFFATGSLLRSKTEP
                     SVIIQADRKTFTEYGHKISISQIEEIGMFGLEDVQEGLVRELRKLVEKEGLKLACLLV
                     TDIVTHDSMLLAVGDEEALENIEYERLGPNLFSATDVVSRKKQLFPAISRALKTL"
     gene            complement(8917..9891)
                     /locus_tag="PK077_01525"
     CDS             complement(8917..9891)
                     /locus_tag="PK077_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23015.1"
                     /translation="MGIALGGVSLCLWVIVVTIFWTGSDTMAFATSLPIWACCLIFSF
                     PALIAWLVFGSRIGLLGIIVWFAYGIVITDFLPPISRTGMEYYKMPPSPDARQIRVLT
                     LYEGCEKSPPIEQISKLRADVIFVQGCSNYNRTLKFAYSIFGRTSYIKQIGSCAIIVR
                     HGQIGPAQSITDTAGLIVDWIPENSSLAIRLINISLEPFEHRFDLYSPACWKYFHTLR
                     FIHRKQLQYLFDTLREVGTQHGELPIIMAGNFSVAPQSPIFSKLSSDFQDCFKLKGAG
                     YGATQPVNFPLLRLDRVFCTPPLKPERASTVLIPDTIRRSIVADIAIP"
     gene            complement(9970..10284)
                     /locus_tag="PK077_01530"
     CDS             complement(9970..10284)
                     /locus_tag="PK077_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRN23016.1"
                     /translation="MNIMKMMKQVQQMQAGLAAAQEKLASQTVTTEGAGGKLKVTATC
                     DGNLTELVIDPSIINPSDSEFLQELLLQTINAAIAKGKETAAAEMKKLTGGLGLPPGM
                     GF"
     gene            complement(10313..10960)
                     /locus_tag="PK077_01535"
     CDS             complement(10313..10960)
                     /locus_tag="PK077_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23017.1"
                     /translation="MSKFPTDSPEPTFMDPEENLPLERRTNSFFDNLFDTPSSSSRTQ
                     APVEKEEPAVLSQQSGRTITEEDWKTVLEQMSAKFPLQADFLANSVFSGYDGVAVAIS
                     FHPSDRQGMDSLGAGPLRTALEAALSQYIGTSVTISIRQDFSIPEPVQEELAPLPAPP
                     PSASTIPAPKPHTPQKKNAEPVQEASKEENEDNSYYTDPLIDAAMEIFRARIISQ"
     gene            11434..12012
                     /locus_tag="PK077_01540"
     CDS             11434..12012
                     /locus_tag="PK077_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23018.1"
                     /translation="MNGFNQAHFQMKPFIGGPAHFIFRFRQNFHQTQDFVRMGAFDQF
                     GEFLLGGFRERIKAGFWIYFRDQNFTHGGDNFGKELAHVSSCFRLFMQKMQERGKFLV
                     QNKRQSMGHGLPGSNAENVEYLLFFNMISTEGNKLVQHGLCIPHTSIRHFGDSKGCFP
                     AQTYSLCRSDMEQVFCNDFRRDGAQVEALASR"
     gene            12730..13506
                     /locus_tag="PK077_01545"
     CDS             12730..13506
                     /locus_tag="PK077_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23019.1"
                     /translation="MWVKVSIAALVIALLGGAMYYLDNEEALTKERRECASRYKNLNG
                     LRGEFTEEKTKYVDFLVKNEALTNDINALTKEKDELEVKNQELASANEAKKSDLQTQQ
                     TALAELQSKSKDMESIQAIADRIKGLEEESKQLEVVKQAEQGKHDAIVAETEQLVVNN
                     AALRQLKADQDARLSPPNLKTRVSQVIHDFNVVVIDGGAADLGVVPGSKLAVMRDGNK
                     IAELDVNAVESRVSTATILPSTVTAGERVEAGDVVVSVRP"
     gene            13554..14369
                     /locus_tag="PK077_01550"
     CDS             13554..14369
                     /locus_tag="PK077_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23020.1"
                     /translation="MKTVLVVLLILAAGAGAWFSGLSGLFGINGQYQDFKEMISYRKT
                     LDKEIRTWTSLRNDMIKNRGLAAEENLSLISNKASVTQQRDEQLSKEAELKEEEVQLN
                     ADLSKINKQIEELKSKLQDFGVSSVQEIQEKMESLEASNKKLQEDIDQIKSATEVASK
                     RRAEQASELASRQKEQAEYRAALAKNGEEYPVLSVDPQWGFVVIGAGQGSSIDPNTVL
                     LVTREGRSIGKLKVTSLEKNQTVADIIKDSVPSGMSIQPGDRVQLLRPRQSAK"
     gene            14731..14823
                     /locus_tag="PK077_01555"
     tRNA            14731..14823
                     /locus_tag="PK077_01555"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14766..14768,aa:Ser,seq:cga)
     gene            complement(14908..15648)
                     /gene="radC"
                     /locus_tag="PK077_01560"
     CDS             complement(14908..15648)
                     /gene="radC"
                     /locus_tag="PK077_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRN23021.1"
                     /translation="MTYRKLQELPSQSLPREKLLRQGRHSLSNAELLAIFLRMGIKGK
                     NVLDMSSDLIQSAGSLDALAHMEAAEIANICKGIGMAKAATLSAAFELGARALRETLS
                     RQPIQTPEDVYDYLTTAMRWKEKETVLVLLLDTKCRLIKPVEISIGTLNESIAHPRDI
                     LRPTVIHNAYGFILAHNHPSGNPAPSRTDDLLTERVRECSKLLGVRFLDHVIIGKPTE
                     TTNKNYYSYNHPGGDRLKDPSQERTLYH"
     gene            complement(15650..17140)
                     /locus_tag="PK077_01565"
     CDS             complement(15650..17140)
                     /locus_tag="PK077_01565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRN23022.1"
                     /translation="MKVCSTENMQIAERELIVSGTPARTLMKLASSGIAESLMQFFPD
                     PGLCVAYVGKGNNGGDALTVLNILKQHGWEIGFRTAYPRNEWSELSVRQLAEISPPPQ
                     EYQAPPLPRTGKPMILLDGLLGIGAKGMLRREISALCAEMNYIRNRCGAVRTVAIDIP
                     TGVDPDTGMPQQNAVEADFTMCIGAVKQGLLDDDATLFAGRLVCIDLPGLHVQALPAT
                     ELITSSRLTKFLSARPYTDYKNKRGHIGVIAGSEGMLGAARLCCEAALRAGAGLVTLH
                     VHKDVYPLIAPSMPPEIMVRPVDSYADISIRTFSAFLIGPGIGSVSEEDAEAIRLILE
                     TGTPTVLDADGLNLAAAMQWNLGEHVLATPHHGEIRRLLPDADNYAIRADIADCFLAE
                     HEAALIYKGARTIVTQRGKPLFYNITGDPGMATAGQGDVLAGICGGFISQGESLLVSA
                     VLGVYLCGRASEMAISAGEATQQTLTAGDTLRHLPSAILSTARLCY"
     gene            complement(17234..>17335)
                     /locus_tag="PK077_01570"
     CDS             complement(17234..>17335)
                     /locus_tag="PK077_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRN23023.1"
                     /translation="MRKRFIKIVVGDKVRMEMSPYDMTKARITFRIG"
BASE COUNT         4184 a         3825 c         4913 g         4413 t
ORIGIN      
        1 cggctgaaga tgtagtggcg cgtgtaactc cctcggcaaa gggggctgtc ttgttccact
       61 taaatccgga tcagggcaac aagatcacta tttccggtat tgccggaggt atccgggtgg
      121 aggcgtcaga tgtgcgccgc ttggtggcgg ggtatggatg gtatttaaag aatatagcca
      181 aggttcattt ttcgtggaac ggaaatcgga taacgcttcc ttcccctttg ccggttcccg
      241 ccagtccggt tacggtagaa agcccgtgga atatcgtttt tgcctataat tactgcaccc
      301 tttcttatac ggcggctttt tgggattgga acagatggca gcgggagatt gattttctgg
      361 cgttgaatgg gtttacgcat gcgctggtaa cggcgggttt ggaaaagaca tgggaagatt
      421 ttttgacagg cttggggtat cctcgggaaa aagctcttcg ttttatccct aatccggcat
      481 ttgccgcgtg gtggaatatg ggaaatctgg aaggccacgg tggtccgctg agccagcaac
      541 agataaataa aatggcccag atgggaaggc gcattgtctc ccgtatggaa cagttgggga
      601 tgacacctgt acttcagggg tatgtgggat ttgttccttc tgactttcag gaaaatgtcc
      661 ggatagacgg attgaagctt attcctcagg gggaatgggt taatttcagg aggccgtggg
      721 tggtggatcc cacttgcgag gcttttccca aactggccgc ggactggtac aaggctctcc
      781 gcaaggtata cggcattcct ggaaagatgt ttggcgggga tttgtttcat gagggggggc
      841 ggaaggggga tattgacgta acgcaggcgg cacaggcagt gcagaaagcc atgcaaaagg
      901 cttctccggg ggctttctgg gtgattcagg cctggggtgg aaatcctacc cgagagttgt
      961 tgtccgggtt ggatccggag cgtgcgcttg tattgcagct taccaaggat atggctaacg
     1021 ggggaaagaa tttaaggact tttaatggta ttccgtgggt ctggtgcgaa ctggcgaact
     1081 ttggaggcaa tacgggaatg tacggggggg ttccccttct gtcccggctc ggaagtgagt
     1141 tgtccggcta taaggataag ggactggtgg gcatgggaac tttgtccgaa gggcttgaga
     1201 cgaaccccct gcattacgcc ctgttcagcg accgtttgtg gactagggag gatatttctg
     1261 tccgggagtg gcttggaaag tacgcgcgcc agcgttacgg tttcgcacca aaggctgtcg
     1321 tgaaggcgtt ggaagttctg tcttcctcca tctataaccc cgttcgttct caggaagggt
     1381 gtacggagtc tattatttgc gcccgcccgt cctggaatgt tcgaaaggca tccacatggt
     1441 ccagcgggga gcgatattat cacctgggcg atattgtaaa ggcggcgcgc ggttatctga
     1501 aggccgcgaa tgatcagccg aatttggtga aaaaggagac gtttcgttat gatttggtgg
     1561 atgttgtgcg tcagtctctt gctttttacc agcttcaaca ggtcaggagt gcttttgatt
     1621 ccggagattt ggctgcgtac aggaaacagg taaagcgttt cctgtctctg atttcagata
     1681 tggatgccct tttggcgacg gatagccagt tcctattagg aacttggcag aaaagggctt
     1741 tggattgggg tgattcccgg caggaaaaag ccttgatgga caagtctgcg aagatgctta
     1801 tcactacgtg gattgatcag gttccccggt ctctgaatga ttattccaat cgtcagtggg
     1861 ccgggcttgt ttccgatttt tatttgcctc ggtggaagaa tttttttgaa tttcagatgg
     1921 atgtcctgac cggaaagaag acgcgtgatg ccgcccatgc cgcatttatg gataagatgg
     1981 ttcgggatga actggccttt gccggaaatg ggaaaatata ttctgccaaa ccggcggggg
     2041 atactttggc cgttgctaat cgtgtgatga atacccaccg ggaaatgttg gacgccctga
     2101 gtgcggaaga aaagcattcc tcgggcagtc catgggaact ccaacaaggt tctccgttgc
     2161 agtttgatgt gacggatcag gtgactgctt ctggcacata tactgctact ttccagtgga
     2221 agaatggccc cagcgccttg aaaatccatt ctgtcagatt atacgagggt aacagggagg
     2281 tggcttccga tgttcatgag ggcagaactg gcgtggaaaa taaggataat atttacagac
     2341 tggaattgaa gaagtacaga accaaccttg attcctatat cctgaaggca gaagtaagcg
     2401 gtgtttctca gggcgcttcc aaaggagaaa tggtgttgaa aaaactggtg gattgagaag
     2461 tggacaggaa gttgtgcagg gatgggagaa aaggcggtta tacgtgcagg cttttccatt
     2521 tgtttcggaa tcagtttgtt tccctcgccg aaaaaaggaa gaaggggggg attttccccc
     2581 ctttcttcct tgaaagatgg catggtgata aaatttatcg gcggtggcgt aacaatagag
     2641 cgctaagacc gaacagaccc agcgtagcgg ttgccggttc cggaaccact tcattacttg
     2701 tggagaagtt tactgccgac aaatacatgc cctcccaaga gttcaggata ttgtttttat
     2761 aggtgccggt tccgcagccg ctatgcatgt ccagagaaag gctgtttaaa tagacggttt
     2821 cgggaagtgt tgcttccttt ctattcgtgg cggcggacat aacggaagaa gacgattggg
     2881 tcgcagagcc aaagtagttg ttggcggtgt tagtgttcgt ggtggagaat atggtggctg
     2941 cttgggcggt tcccaacgat aaaaaatgga gataacagaa ataatgggtt tcatctttgg
     3001 gctggaaata taacttgata caattgatat gcactgggaa aaaaatctat cgaatttaga
     3061 atcgtttttt gaaaaacatg aaatatattg atgttataaa gaaatattca atttatttaa
     3121 aattttagat ggaaaacgat tagtaatagt cttttttatc tcattatcgg tattatccga
     3181 gaaaaaaatc atgacccaat ggtaaatacc ggttctagat tttctttact ccggcacata
     3241 gccaaaattc cgtcctcaga aaactcctgc ttcccgaagc ggaatggtca gcctacacaa
     3301 gctattctct tttagaagta aatattgacc tcggattcgc aatccgtttg tcgatattct
     3361 tattgtactg ccccgattta tgctccggtt ccaaaagatg agctgtacag gatcttttgt
     3421 agagatggat tttttttgaa tatagccttt tcgttcagag ggctgcctgg taaatgctga
     3481 acatcagaat ggccattccc gtgagggctg cgcctgcaat gatgccggag gcggtgggga
     3541 ctttatattc ccgtgatttg gccttgaaaa gatggtccgc agcccagccg agcacagctc
     3601 ctgtaaagaa aaggaaggag tcgttccact ggatggccca ggccaggccg aagccgatgg
     3661 gggtggggag ccattttcct gtccggggaa tatagcgggg aataagcacc agaatgagtc
     3721 cgagaatacc gcctgcaatg atggaaagca ccttgatgct gtgcaaatgg ctgaacccca
     3781 tgcccagtgc ttccgctgtg gccgcccatg ttctggctcc cggagcattg aaagcctgga
     3841 gagcgttcgc atccggaacc atggagcgga aggccagcac ggagacgagg gttccgagaa
     3901 aaattccgga aaactgggcg atgaattgtt tgcgggggtt agctcccagc aggtagccta
     3961 ctttgaggtc tacgagcagg tctgccgagg aactggacgc tcctgaagtg atattggccg
     4021 ccatcaggtt ggctgttacg tgcccgggtg caatccctcc gaagatgagc tgtgtaactt
     4081 ttcccatggc tccggtgggt gtaatattgg cttctccggt gattcggcag acgaccagcg
     4141 ccaggaaaaa ggatatgact accgcgatgc agctcatcca gtacggaacg ttaaaggatg
     4201 tatgagccag atagccgaga gctcccagag agataagctg gcctgtcaga aaccagctca
     4261 tgggcgtttc tattttttcc acatctgtca gttttcgttt tttactcagg gaaaacatga
     4321 cacccaggga agaaaaggaa cgaacaatgc ttttccattg gaataggaaa gccacaatgc
     4381 tggcgacgac catgcaggcg gttcctcccc acagggtcca gcttacgctc tcccgataac
     4441 ctgctcccgc gggaagcaat ttctggcttt ccagccacgg aacgtaaaga gcccagcaaa
     4501 cggttcctcc gatgaagagg ctgagggatg ttttcatgcc taccagagct cccgcaccca
     4561 cgaacatgag atcccatttg aagaagatag tacgctggta ccagttgccg aggatatgtt
     4621 gctggaccgt tccaagccat cgttctcctc ctgcagccat ggcgatggca ttagcggcgg
     4681 cgaaaagggc agaataaaga agggctttgg cctgtcccgc cgcctttttg ccttcagaat
     4741 agagaacttt gagggtttcc gccgtagcaa tactgtctgg gaaccttatt tgttcaatgt
     4801 tgatcatctg gcgtttcatc ggaatggcca tggtgacacc cagtacggca atgaaaaata
     4861 tccaggcgaa ggtagtgcca agaggcatgt gctgtcctgt cagcaggagc agggcggcta
     4921 cggcagaggt gagggttccc cctgtagaat agcctgcaga actggcagcg gactgcatgc
     4981 atgtattttc cagaatagtc atgggggatt tggaaatgcc caggcgcaca aaggcgttcc
     5041 agagggcaaa agagataatt cctgccgtca gagccacccc aaaggaccat cccattttga
     5101 ggttggcgta cagattagtg agggaaagga tggagcctag gagcatgcct acgatgacgg
     5161 ctctccaggt aagctgcttc attctgtctc cggaacccag ataaacttga tcgtaccatt
     5221 gctgttctac ttcatccgga gtaccttgga atccatccag aggcagcggt ttttccaagc
     5281 aggaggggag agggatggaa gactgggagg acgtagatgc catacagttc ataatatagc
     5341 atttgcgggc gtggtggaaa agtcgtatct gtctttctct gctttcggcg ttccttctgc
     5401 ttgagacttg cgtgcttgtg acggccgtgg tttaatgggc ggcatgagcc aatcttttac
     5461 ggataaactt gccgcccgca ttaaagagac cggttccgcc ttgtgtgtcg gattggatcc
     5521 gcgccccggc atggatgatt tggaggcgat tcctgcgctg ttgcggaagg ttgtggagga
     5581 aaccgctcca tatgcggcag cgtttaagcc gaatattgcc tattttgaag ccatggggtt
     5641 gcgcggtctg gaaatacttg aggatttgct tccggatatg cctaaggatg ttccggtggt
     5701 gctggatgct aagcgtggag atattggaga gactcagaag tattacgctc atgcttactt
     5761 tgaacgcctg ggtgtggacg ctgtgacgtt gagtcctttt atgggatatg acacgctgga
     5821 gccttttctg aattatgaag gtaaaggtgt ttatctgctg gcagttactt ccaatcccgg
     5881 ttctgcggat gtggaacggc aggaactggc agggggccgc agggtttttg aactggtggg
     5941 agacatggtg ctccgttctg tccgggaagg ctgtaaaact tccgtaggca tggtggttgg
     6001 tttgaccaat gcagattcca gtattctgga gcgtattccg gatgctcctt tgctcattcc
     6061 cggcctgggt gctcaaggcg gtgatctttc ctgcctgagt ggttccggtc atgtggcgcc
     6121 tcccctgatt aatgtctctc gaggaatcat gtaccagaat ccggaattgg gttttgctga
     6181 gaaggccgcc ggatttgctc agcgcattcg agaggcattg aattattgat gttcatgtat
     6241 tttttgcaaa taacaaaatg ataacggcct gcttatggct gtgcatgagc ttgtcaaagg
     6301 gagggggctg atatattgaa caggtgcatc cccattccct gcccgttttt tatggatgaa
     6361 aagcccggaa ttgtaaaatt cactccccat tccacttcca gatccgcaat gttgattgcg
     6421 gtggcagtga cagtgggtgt tcaggtggct ttgtgtgctg tgcttgtagc catacatttg
     6481 caggatatgt ggtttccctc cgtccagggt ggggaagtgg aaatgacgtt tgtgtccccg
     6541 tccgaagaag tttctcagga gatggaagaa gttcagccta catctgttgt tccggttgca
     6601 gccccttcca cagtcagccc tgttccggag attgcgtcag atgaagactt attggcgatg
     6661 aagctgccgg aaaatgaaga ttgttccgaa atgaaggatt ttcttttgga tgaagctcct
     6721 tccgaactct tgccggagga gctcgccgcc atgcggattt ccatgaatga agtaaagcga
     6781 aggcagcctg tgaagcgccc ggcggtatcc ctgccggggc gtccggaacc ggatcagaat
     6841 atttctcctg ataaaaaggt gcgttataaa catgctccgt ccttgcccaa ttccgtcaat
     6901 tcttctaaag tcgggaaggt gaatgccgtg gtgaaagtag tggttggagt aaattcccat
     6961 ggggaacctt catccgtcaa tcttcttcag tcaacgggaa atgcggaatt ggaccgcctt
     7021 tttatgcgtt gggtgagaga gaattggact ttttatccgg cggaaaagga cggtgtgccc
     7081 attgcttcca aggtggtggt gcccgtcagg ctgaatatag attaacctgt acaaggaaat
     7141 ataaagatgc ttccggagaa gacagagaat aggcccgttt atgtgatcgg ccaccagaat
     7201 ccggatacgg atgctatttg ttcagccata gggaatgcgg agtttttaag aactcatggc
     7261 gaacctggtg cggttgccgc cagatgtggg gaaatgacgc tgagaacatc ctgggtattg
     7321 gaaaaggcag gagtgccgga gccgatactg attcatgacg tgacgcccac cgccgggaca
     7381 atctgtcgtc gtgatgtgat tagcgtaagt ccggacgata cattcctgac ggcgtatcga
     7441 aggatgacgg agaattctct gcaaaccata ccggtaattg acgctgatta caaccttcat
     7501 ggcctattgc gttattttga tttgttgagc ctgctcatgc ctctgaatat gacggagatg
     7561 aatgtgcgct ccgtcttttc cagcctgagc aatatcgcca ccaccattga cggaaaatgt
     7621 ctgaccgggg aatccttaag tgaggaagaa acgcagaata ttctgctggt gggtgcttcc
     7681 agtgaaccca gcgttcgaac aaggctcgca aattacagga gaaaagggat tatccgggat
     7741 ctgattgtta tttgtgggga ccgtccgaat gtgcagctat atgcagtgga acatggcgta
     7801 cgcgctttgg tgaccacgtc cggtgctttc ccttccttgg acattattga gacggcccag
     7861 acgacgggaa catgtatttt gagtactccc tgggatacgg ccagtgttgg ccagttgatc
     7921 cgttgttccc ggaaggtgag ggagcaggtt cagaaggatt acaccgtttt tccggagaat
     7981 atgccgctgc ccgagttgag gcaggctgcc gtaaagcgca agcaggcatt gttccctgtg
     8041 atgagtatca agacgaacaa aataattggt gtactgagca agacggattt ggtggatccg
     8101 ccccgtacac gtgtggcttt agtggatcac aatgaatttt ctcaggccgt caagggggtg
     8161 gaggaggcgg agattgtgga ggtgatggat caccaccgtt tgggtaccca gctttctaca
     8221 cgggatccaa tccgttttct gaatgaaccc gtaggatcca catccacctt ggtggcacgc
     8281 agattttacc atcgtaatgt ggaaccctcc caggctgtag cggtctgcct gtgcgcgggg
     8341 attctttcag ataccctgaa tttgacatct cctacggcgg ctcgggcaga tagggaaatg
     8401 ttggaatggc tgacagggat tgccaaaata gatgccaaga agtttacaga ggagtttttt
     8461 gccaccggtt ctctgttgcg ttccaaaact gaaccttccg ttattattca ggcggacagg
     8521 aagactttta ctgagtacgg gcataagatc agtatttccc agattgagga aattggtatg
     8581 tttggcttgg aagatgtcca ggaaggttta gtgagggaac ttcggaagct ggtggagaaa
     8641 gaggggctga agcttgcttg ccttttggtt acggatatcg ttactcacga ttccatgctg
     8701 ctggccgtcg gggatgaaga agctctggaa aatatcgagt acgagcggct tggccctaat
     8761 ttgttttccg ctactgatgt ggtaagccga aagaaacagc tttttcctgc tatttcccgt
     8821 gcattaaaga cgttgtagat gccggaaatc tttgcttttt acaggcaaga tttttcctga
     8881 ggaggaacgg cgttgccgtg ctgtaagggg tatagatcag ggaattgcaa tgtctgctac
     8941 gatagaacgg cggatagtat ccggtatcag cacagtactg gctctttcag gtttgagcgg
     9001 cggcgtgcaa aaaactcggt ctaggcgtaa tagagggaaa ttgacaggtt gcgtagctcc
     9061 gtaaccggca cctttcagtt tgaagcaatc ctgaaagtcg gaactgagtt tggagaaaat
     9121 tggggattga ggggcaacgc tgaagttccc ggccatgata atgggaagtt ctccatgctg
     9181 ggttcctact tccctcaggg tatcaaacag gtattgaagt tgtttacggt ggatgaagcg
     9241 gagtgtatgg aaatatttcc agcaggcggg ggagtacagg tcgaagcgat gttcaaaagg
     9301 ttccagactg atgttgatga gccgaatcgc caaggaggaa ttttccggta tccagtctac
     9361 aatcaaaccg gctgtgtccg tgatgctctg ggccggacca atctgaccgt gccttacaat
     9421 gatcgcgcaa cttcctattt gcttgatata agaggttcta ccgaaaatgc tgtaggcaaa
     9481 tttgagtgtt cggttatagt tgctgcagcc ttggacgaaa atgacatctg cacggagttt
     9541 ggatatttgt tcaatgggag gagatttctc gcacccctcg tacaaggtaa gaacccttat
     9601 ttgcctggca tccggagacg gcggcatttt gtagtattcc attccagttc tggaaatggg
     9661 cgggagaaag tctgtaataa cgatgccgta tgcaaaccag acgataatgc caagcagacc
     9721 gattcgggaa ccgaaaacca gccatgcaat gagcgccgga aaggagaaaa tgagacagca
     9781 agcccatatg ggcagtgacg ttgcaaaagc catcgtgtct gaaccggtcc agaaaatagt
     9841 gacgacgatg acccacaggc acagagagac gccacccagc gctattccca agtggttgac
     9901 tccggatagc gttggcagag gttctgccag atgtttgcgt cttctgagcc gatgcactgt
     9961 ggtgacagat tagaagccca tgccgggagg gagacccaag cctcctgtca gctttttcat
    10021 ttccgccgcg gctgtttcct tacccttagc aatagctgca ttgatggttt gaaggagaag
    10081 ctcctggaga aattctgaat cggacgggtt aatgatggaa ggatcaatga ccagttccgt
    10141 gaggttgccg tcacaggtgg cggtaacttt caattttcct ccagctcctt ccgttgtaac
    10201 ggtttgggag gcgagttttt cttgagcggc cgcaagccct gcctgcattt gctggacctg
    10261 tttcatcatt ttcattatgt tcatggtcgt tgtgtctggg tggatgagtt gactattggg
    10321 aaatgatacg agcccggaaa atctccatag cagcatcaat cagcggatcc gtgtagtaag
    10381 agttatcttc attttcctct ttggaagctt cctggacagg ctctgcgttt tttttctgcg
    10441 gggtatgagg tttaggtgca ggaatggtgg atgcggaagg aggtggtgca ggcagaggag
    10501 ccagttcttc ctgcacgggt tcgggaatgg agaaatcctg acggatagaa atagtaacag
    10561 aggttccaat atattgggaa agagcggctt ccaaagctgt tcgcagaggg ccggctccaa
    10621 gactgtccat cccctgacgg tcagaaggat gaaaagaaat ggcgactgct acgccatcat
    10681 aaccggaaaa aacgctgttg gctagaaagt ccgcttgaag agggaatttg gcggacattt
    10741 gttccaacac cgttttccag tcttcttccg tgatagttct tcctgattgt tgggacagaa
    10801 ctgccggttc ctctttttct acgggtgcct gggttcggga tgatgagctc ggagtatcga
    10861 atagattgtc aaagaaactg ttagttcgtc tttccagagg aagattttcc tctggatcca
    10921 taaaagtagg ctctggagaa tccgtaggaa atttcgacac atcggaggca tcggagtctg
    10981 gatgccgggt gatttcttcg gaggcaggta cggtttttgt ccgcaattta gtggcggagt
    11041 agggaaattc ttcctctgca acggcaagag aggggggggc tgcagcgggt gtaagagttt
    11101 cctgttgtgg ttcggctcct gcgtaggatg gggagaagct tgagcgtcag tgctctgaat
    11161 tttctgtact gcgggagatg aagcagggga ggacacagaa aaatctggaa tcggatccat
    11221 caaatgttct tccgtagctg gggttgggtt tgttgcagca acaggagact ggatgttttg
    11281 ccggggggcc ggagtgggtg ccgtggcgga ggaaataaat gtacttggtt cctgctgtga
    11341 agcaagatct gaagaagagg ccggtgccgc ggtggctagc ggagcacctt ccaacgccat
    11401 gataatatcg ctgatgctgg cctcagccag agtatgaacg gctttaatca ggcccatttc
    11461 cagatgaagc cttttattgg tggaccagcg cattttatct tccgtttccg ccagaacttc
    11521 caccaaacgc aggattttgt ccgtatgggt gcgtttgacc aattcggcga gttcctcctt
    11581 ggaggattcc gggagagaat caaagctggc ttctggatct actttagaga ccagaatttc
    11641 acgcacggcg gagataattt cggaaaggaa ctggctcatg tctcttcctg cttccgcctg
    11701 ttcatgcaga agatgcagga gagagggaag ttccttgttc aaaataagcg ccagagcatg
    11761 ggccacggtc tcccgggaag taatgccgaa aatgtggagt acctgctgtt cttcaatatg
    11821 atctccacag aaggaaacaa gctggtccag catggattgt gcatcccgca tacctccatc
    11881 cgccactttg gcgacagcaa aggctgcttc cctgctcaga cttactccct ctgcagaagc
    11941 gatatggagc aggtgttctg caatgatttc agaagggatg gggcgcaggt cgaagcgctg
    12001 gcatcgcgat agaatggttg gcagtatttt gtggggttcc gtggttgcga aaataaattt
    12061 gacatgaggt ggcggttctt ccagcgtttt gagcagggcg ttgaaggatt ccttagtgag
    12121 catgtgcact tcatctatat agacgatacg gaaatttcct ctgctcggag cgaaccgtac
    12181 gttgtccctg agatcccgga tgtgatcaat gccgcggttg gatgctccgt ctatttcaag
    12241 aacatccagg ctccgccctt ccgcaatttc ttcacatata tcttcatgcg ggtcaaaatc
    12301 cactttgggc ccaccgctgc agtttagagc tttcgcaaaa attcttgcgg ttgatgtctt
    12361 tcccgttcca cggggaccta caaagagata ggcatgggcg agacggttgt gctcaatggc
    12421 gtttttcaac gtccggacaa catgatcctg tccaaggacg tcatcgaatg tcagaggacg
    12481 gtattttctg gcaaagacct ggtaactcac ggttcagaaa gactagaaga tgagttcccg
    12541 tttgaaaaga aaatagagag taattgtttt aaaatgttga taaaacggtt cttgcacact
    12601 cccgttacgg agcttgcctg aaaaaaatat caagaaaaca atgaggatgt cattttgata
    12661 ttgaaatctg aaaatgttca tgccaatctc acaaaagtaa caggaacctt ctcatctcta
    12721 taatttccta tgtgggtcaa agtatcaata gcagccttgg tcattgctct tctcggagga
    12781 gccatgtatt atcttgataa cgaggaagct ctaaccaagg agcgtaggga atgtgcttcc
    12841 cgctacaaga acctgaacgg ccttcgtggc gaatttacgg aagaaaagac gaaatatgtc
    12901 gatttcctgg tgaagaatga ggctctgacg aatgacatca atgctttaac caaggaaaag
    12961 gatgaactgg aagtaaagaa tcaggaattg gcctcagcca atgaggccaa gaaatctgac
    13021 cttcaaacgc agcaaaccgc tttggcggaa ttgcaatcca agagcaagga tatggaaagt
    13081 atccaagcca ttgctgacag aatcaagggg ctggaagaag agtccaagca gttggaagtg
    13141 gttaagcagg cggaacaggg aaaacatgat gccatcgttg ctgaaaccga acagcttgtt
    13201 gtgaataatg ccgctcttcg tcagctcaag gccgaccagg atgcccgcct ttctccgccc
    13261 aacctgaaaa cgagggtttc ccaggtgatt catgatttta atgtagttgt gattgacgga
    13321 ggagccgccg atctgggcgt ggtgcccggt tccaagttgg ccgtcatgag ggacggcaac
    13381 aaaattgccg agcttgacgt aaacgctgtt gaatcccgtg tttccacggc cactattcta
    13441 ccaagtacgg taaccgccgg cgaacgtgtt gaagccggag acgtcgttgt atctgttcgt
    13501 ccctagcatt tttccggttc aatacactcc atacactctc acatacatac acaatgaaaa
    13561 ctgtcttagt agttttattg attcttgcag caggggcggg cgcctggttc tccggtctta
    13621 gcggcctttt cggtattaat ggccagtacc aggattttaa agaaatgatt tcgtatcgta
    13681 agaccttgga caaggaaata cgtacttgga caagcttgcg caacgacatg attaagaatc
    13741 gtggcttggc agcggaagaa aatcttagtt tgatttccaa caaggcttcc gtgacgcagc
    13801 agcgtgatga acagctttcc aaggaagcgg agttgaagga agaagaagtg cagttgaatg
    13861 cggacttgtc caaaatcaat aaacagattg aggaattaaa gagcaagttg caagatttcg
    13921 gtgtttccag cgtacaggaa atccaggaga agatggaatc tttggaagct tccaataaga
    13981 agctccagga agatattgac cagattaaaa gtgctacgga agtggcttcc aagagacggg
    14041 cagaacaagc ttctgagctg gctagccgcc agaaggagca agctgaatac cgagctgctc
    14101 ttgccaaaaa tggggaggag tatcccgttc tatccgtgga cccgcagtgg ggttttgttg
    14161 tgattggggc tggacagggc agtagtattg atccaaatac ggttcttctt gttacccgtg
    14221 aaggtcgcag cattggtaaa ttaaaggtaa cttccctgga gaaaaatcag acggtagctg
    14281 acattattaa agacagcgta ccgtctggta tgagtattca acccggcgat agagtacaac
    14341 tcctgcgtcc ccgtcagtcc gccaaataaa ggtttgccac gtattttcct tgaaccccgt
    14401 tgtgtcgttc ggcataatgg ggttcaattt tttaaaagga aatgatgaaa cgatttgcaa
    14461 tctccttttt gtttgtgtct ttttctattc ttctggtctc ttcctgttcc tggttgatgg
    14521 aaccgcagga gagggagcgt gtgccgcagc cgcctcccgg tccggaaaag agtgcgcttc
    14581 ctcataataa gacccaaaga tttgaggcgg aagcaacgct cgggccgttg gcgtcgccga
    14641 gacgttaacg gcatccgaat gacgctggat ggtgattctg ttctttacag tatccgacaa
    14701 accggataca accgcctccg gtttccaagt ggagagatgg ctgagctggt ctaaggcact
    14761 cgactcgaaa tcgaacgtgg gctttaaaac ccaccgtggg ttcgaatccc actctctccg
    14821 ccagtttttc agtaagagcc ccgtcggaag acgaggctct tattttcgat aggaagagaa
    14881 caggcgggaa ttcagatagg cggctgttca gtgatagagg gtacgttctt gactggggtc
    14941 cttaaggcgg tctccaccag gatggttgta actataataa tttttgttgg ttgtttcggt
    15001 cggcttacca atgattacat gatccagaaa acgaacgcct aataatttgg agcattcccg
    15061 cacgcgttcg gtcaggaggt cgtccgtacg gctgggggct ggatttccac tgggatgatt
    15121 gtgggcaagg atgaagccat aggcgttatg gatgacggtg gggcgtaata tgtcacgtgg
    15181 atgagcaatg gattcgttca aggtcccgat ggatatttcc acaggtttga tgaggcggca
    15241 cttggtatcc agcagcagaa cgagcaccgt ctccttttcc ttccagcgca tggctgtggt
    15301 cagataatca tagacatctt ccggtgtttg aatgggttga cgtgacagag tttcacggag
    15361 agccctcgct cccagttcaa aggcagcact cagcgtagcg gctttcgcca tgcctattcc
    15421 cttgcaaatg ttcgctattt cagcagcttc catatgagct agggcatcta gagagccagc
    15481 agactgaatc agatccgagg acatgtccaa cacatttttc cccttgatcc ccattcggag
    15541 aaagatagca agcagttccg cgttgctgag gctgtgcctt ccttggcgga gcagtttttc
    15601 cctgggcaga gactgtgagg gcaattcctg aagtttgcgg taggtcattt caataacaga
    15661 ggcgtgcggt agaaagaatg gcggaaggca gatgtcgcag cgtatcccct gccgtcaatg
    15721 tttgctgggt ggcttctccg gcggagattg ccatttcgga agctcggcca cacaggtaga
    15781 cgcccagaac ggcggaaacc agcagggatt ccccctggct tataaagcct ccgcaaatac
    15841 ctgccagcac gtctccctga ccggcagttg ccatgccggg atccccagtg atgttataaa
    15901 aaagaggttt tccccgttgg gtgacgatag tgcgtgctcc cttgtaaatc agggcggctt
    15961 catgttctgc cagaaagcag tcggcaatgt ctgcccggat ggcatagttg tctgcatctg
    16021 gcagaagtct gcgaatttct ccatggtggg gagtagccag aacgtgttct cccaagttcc
    16081 actgcatggc cgcggccaga ttcagcccgt ccgcatccag aacagtgggg gtacctgttt
    16141 ccagaatgag gcggatggct tccgcatctt cctccgatac ggaaccgata cctgggccaa
    16201 tgaggaaagc actgaatgtg cgaatggaga tatctgcgta actgtccacg ggtctgacca
    16261 tgatttccgg aggcatggat ggagcgatta agggatagac gtctttgtga acgtgcagtg
    16321 taaccagacc ggctcctgcc cggagagctg cttcacagca gagacgggcc gcacccagca
    16381 ttccttcaga gccagctatg acgccaatgt gcccgcgttt gttcttataa tccgtatagg
    16441 gtcttgcaga gaggaatttg gtaagccgtg aggaggtaat aagttccgtg gcggggagcg
    16501 cctgtacgtg aagaccggga aggtcaatgc agactaagcg tccggcaaac agggtggcgt
    16561 catcgtccag caggccctgc ttaacggctc ctatgcacat ggtgaaatct gcttccactg
    16621 cattttgctg aggcattccc gtatctggat ccactccggt ggggatatca atagccacgg
    16681 tacgtacagc cccgcaacgg tttcttatat agttcatttc cgcgcagaga gcggagattt
    16741 ccctccggag cattcccttt gcccctattc ccagcaggcc gtccagcagg atcataggtt
    16801 ttcccgtacg gggaagggga ggggcctggt attcctgagg gggaggagat atttccgcca
    16861 actgccgcac ggaaagttcg ctccattcgt tgcgcgggta agcggttcgg aagcctattt
    16921 cccagccatg ttgtttcagt atgttaagga cggtgagagc atctcccccg ttattccctt
    16981 tgcccacata ggctacgcac aatcccgggt cggggaagaa ctgcatgaga gattccgcaa
    17041 taccggaaga ggccagtttc atcagggttc tggctggtgt tccgctaacg atgagctcgc
    17101 gttccgcgat ctgcatattt tccgtggagc aaactttcat ggcatactga tagaggcagc
    17161 ctttactgta agctaaaaaa ccttctcctg caagccggag aaggttttta aaaagaagcg
    17221 gaccagggag cagctaaccg atgcggaacg tgatgcgcgc tttggtcatg tcataaggag
    17281 acatttccat acgcactttg tcccctacca cgattttaat aaagcgtttg cgcat
//
LOCUS       DAOMSU010000017        17030 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_110_length_17030_cov_3.195228, whole genome shotgun sequence.
ACCESSION   DAOMSU010000017 DAOMSU010000000
VERSION     DAOMSU010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 17030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17030
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110_length_17030_cov_3.195228"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(158..982)
                     /gene="menB"
                     /locus_tag="PK077_01575"
     CDS             complement(158..982)
                     /gene="menB"
                     /locus_tag="PK077_01575"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HRN23024.1"
                     /translation="MSAQWTTAREYTDIKYETTDNGSIAKITINRPHVRNAFRPLTVH
                     EMLNALNAAHEDPRVGVVILTGEGPDAFCSGGDQKVRGNAGYIGEDGVPRLNILDVQR
                     TIRTMPKPVVAMVAGYAIGGGHVLHVVCDLTIAADNAKFGQTGPKVGSFDGGLGSSYL
                     ARIVGQKKAREIWYLCRQYDARQALDMGLVNTVVPLEDLEEETLSWCRQMLRHSPLAL
                     RCLKASLNADCDGQMGLLDLAGNATLLYYMSEEAKEGKNAFVEKRAPDFSKFPRLP"
     gene            1194..>2094
                     /locus_tag="PK077_01580"
     CDS             1194..>2094
                     /locus_tag="PK077_01580"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRN23025.1"
                     /translation="MIHDLLVSGVTVFPGREHFEFVPGINVVVGGNDSGKSHLLKLCY
                     TVAKWSADGGRKSLPEKWAEEQRLRKDLMRVFASRGLAGLTARNRGNAHARVEASMEG
                     DGVPEGMGNLVFDFQAGHEEEGLSIREMPRRFLNVPVVFLAAREVLTIYPSFVQVGSR
                     FPEFLDGGSWDLCRYLDMEAEAEPISTDAGRVVARLEKIIGGKVVKRDGRFFLQRPGQ
                     QPIEMSLVAEGFKRLGTLSYLIRNGSVKKGSVLFWDEPEMNLNASHLPVLVKTLTGLA
                     KTGVQVILSSHSLFLLRELMIQLS"
     assembly_gap    2095..2126
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2280..3077
                     /locus_tag="PK077_01585"
     CDS             2280..3077
                     /locus_tag="PK077_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23026.1"
                     /translation="MSHKPSKAYYVEGVHRFWIDEGYVVCRIECSDYYARQAQNFCGG
                     CKEMDFLLYHPERRDLWLVEVKDYRFDARPKVGELVCALSRKVRDTMFLLRAASVAAP
                     DEEPAEGISLRDFSIQASGAETLHLAFLLEMGMGGVWTDGGLLANVKNLLVGSMRFLD
                     AELVCAPITYPRRIGPWRVTSAHGEVSKRVEIRRARKLAEIQEQRRRERLNAQEEGAW
                     QEKSREDAGSHAAPAEERMPQWKRRLRQRMNGESANHEGRRRKRKRE"
     gene            3332..5692
                     /gene="ftsH"
                     /locus_tag="PK077_01590"
     CDS             3332..5692
                     /gene="ftsH"
                     /locus_tag="PK077_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018290975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRN23027.1"
                     /translation="MVLLIVGVLAFGFFTPESFGLGPRKENLESFEAQYKAGRVVLND
                     PKAPVEVVLSENGSEGVIHALVYRKEIQPKVEMTPFALTYSMSLPDRDKPLLNELSGY
                     RVVESPYRTEEGKNVSLIPEGAQKLSVPEFNRLALEGRIAGGKDGIILAEDGNQNVLV
                     GQIVTRIWPAATGDASVDKQRFERVEVPFTLEFQGDRVKQLLGPDTKFKRESGSWGGI
                     LLNLLPIVLILVILFFMFRAQSGGARGAMGFGKSRARLISPDKNKVTFKDVAGISEAK
                     EEVWELVEFLRNPEKFRDLGATIPRGVLMVGAPGTGKTLLARAIAGESNASFYSISGS
                     DFVEMFVGVGASRVRDMFEQAKRTAPSLIFIDEIDAVGRQRGYGMGGGNDEREQTLNA
                     LLVEMDGFENNSNVIVIAATNRADILDPALLRPGRFDRQVVVNLPDVRGREQILQVHA
                     RKVKMAPGVSFERIARGTSGFSGAQLANLVNEAALLAARKGLKEITEAELEEARDKVS
                     WGRERRSLAINERGRRITAVHEAGHAICLLKTPHSEPLHRVTIVPRGGALGMTMWLPS
                     DDKMHQLRSEMLDQLVVAMGGRCAEQIVFGDVTSGATGDIKSATNLARRMVCEFGMSE
                     KLGLIEYGEHQGEVYIARDLGTRSRNYSESTAELIDSEVRFLVDSAYERAMAILTENR
                     DKLDILTEALMEFETLEGSQVMDILEYGEMKNPPARVTPPPMPSEVEEQPGKDDSGHT
                     EKKETEETCADGAEERKEEEKQEQAERDPSSCTPADESGMDGGEKK"
     gene            5714..6064
                     /gene="rsfS"
                     /locus_tag="PK077_01595"
     CDS             5714..6064
                     /gene="rsfS"
                     /locus_tag="PK077_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRN23028.1"
                     /translation="MVANDAMELARMCARAADDAKAENVRVYDLRGMSSLTDFMVVCT
                     GLSVPHLRAVIRELEEAVREKAGALPTYVEDTPVALWSVVDYIDVMVHIMGQETREFY
                     GMDTLWKDAPVVEY"
     gene            6380..8881
                     /gene="mutS"
                     /locus_tag="PK077_01600"
     CDS             6380..8881
                     /gene="mutS"
                     /locus_tag="PK077_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRN23029.1"
                     /translation="MSSSGPASTPMMDQYLRMKKGLPEDVLLFFRLGDFYEMFFEDAK
                     EASAILGLTLTKRHGIPMCGVPHHSAEGYIGRLVKGGKRVAIAEQTTIPQPGKLVERE
                     LTRVISAGTLADMNLLDSSRHNYIVALYRDKKRFGLACVDHTTGEFSVAQFEHMDLLL
                     DELSRINPSELLVSDEQTDCFPGTHPTLYYDGYTFLPSTAIPNLLSHFRVHSLDGFGC
                     GEMTAALGASGAVLHYLSYQLRRPTDHLRRISVRAMENAVLIDQASQRNLDLVDSRGG
                     VKLSLLGTLDRTSTPMGARKLRDWLLHPLCDLEKLLARQEVIAVLLQEPYLMSKLRES
                     LKNVRDMERLTGRISQGAGNARDLQALASSLSRIPALRDDLESLPGGGEMLESIRSRM
                     GCFDELVDLLQRALVEEPPVTIKEGGIIREGYHAGLDELRLASRDGKEWLARLQEKER
                     KRTGIDSLKIRFNNVFGYYIEVTKSHYDKVPPDYQRKQTLVNAERFITPELKQMENTI
                     LGADERSRQVEYEQFLLLREEVGRHIDDIQMTADAMADLDVLLGLAEGAQQYRYCRPV
                     LDNSMTLRIVNGRHPVIEQNVSGDVFVPNDAFLEPEENRLILLTGPNMAGKSTYIRQV
                     ALITLMAQIGAYVPAEAAHIGLVDRIFCRVGASDDLARGQSTFMVEMSETSLILNNAT
                     ERSLIILDEIGRGTATFDGLSIAWAVAEYLHDELKSRTLFATHYHELTDLANSRQGVQ
                     NYNVAVREWKEEIVFLRKIVPGAADKSYGIQVARLAGMPAVIVDRAKAILSHLEMNST
                     RPRKKGRSRLAEPRAKNTDMDDDMPVGEYAQLELF"
     gene            8883..9476
                     /locus_tag="PK077_01605"
     CDS             8883..9476
                     /locus_tag="PK077_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRN23030.1"
                     /translation="MPPLSDTIGGLRFAAIDFESAGAARGETDQPVQIGIAACSRLED
                     EPELWTSYIAVEKPVLWSASQVHGITTEMLADAPSFPSLWPEIRSRLGQAVVVGHNPA
                     TERKFLRRFPGHGFGPWLDTLALGRMCVPGLEDYSLSSLCGALGLTCSVEALLPARRW
                     HDALYDALASLLIVRFLVKELNLSGQPLEVLGKAVRT"
     gene            9528..10259
                     /locus_tag="PK077_01610"
     CDS             9528..10259
                     /locus_tag="PK077_01610"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRN23031.1"
                     /translation="MNASVFSAVFLLLSFASSSVYAAPADEAPDPPDVPKLNTQPWVP
                     ARYRAVRENLLGKECGILFVGDSITECWEREGAALWKRLFLPMKAVNFGVSGDRTDSM
                     LWRMEDTKLAVKTSPRYCVLLAGTNNIGRWKGRQAAAQTVKGIREIAVRLLKKFPETR
                     LILMEVTPYGSDPGSPLRRRQEEINRLLRRLELPRTTILSINGSLLKPDGTFREGMFR
                     DHVHLTEKGYQAWADALLPLLNGEG"
     gene            complement(10334..12649)
                     /locus_tag="PK077_01615"
     CDS             complement(10334..12649)
                     /locus_tag="PK077_01615"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRN23032.1"
                     /translation="MRNFLKAAVGCLGMIAGCFSTGEAQDFGGMSFGEAGNFGPPQTS
                     GSSKATVTSYGEASFVIVTELALPDHWHVYYKNPGTVGLPMEAAFQPVPGFRVEGPFW
                     QIPELGKGLVDFYGYSGKAKMAFRVTPEKDAPPEAMFTTTMTWQMCAEQCAAPETRNF
                     SVTLKRGDGQTAPDAAELTGNMAGLAAPDWAEGLKARISQEGKTVTLHLRTNGRPVPQ
                     DSVYFFCNQGEINPTTPQTFKKLDDSNYELSMQFNDTTDGLYPNNLPDADKGKPLTSL
                     SGILRAGREGIIITADDRPFSGESQATAAGTESAPESSASIPAPPLMGLGEIMFFMFI
                     GGIILNVMPCVFPVIGLKIMGFVQLGGGERKKVLAHSLTFVLGILISFWIITAILIAL
                     KANMFDWSAPAGPGVFSGDFWLGRGAEGVVNWAFWFENPWVNFCLLGLMLAMGLSMFG
                     VFEIGVKATTMGNDLQHRKGYAGSFWSGALATVISTPCSAPFLGQAIGAAMLQPPLGI
                     VLCLTMMGLGMSLPYIILGAFPVLTRYLPKPGAWMESFKQAMSFLMFGTAVYFLWIYI
                     AFFDAENHPQDILFLFFGLVLFSMAFWVYGRWCPMYRSRKSRITGGIFSVIFLLAGLY
                     YMLPPEGAAWFGRGSAPGAAESSAAAAPSLQEEGNIWIPWSPEAMQAALDGGKPVYVD
                     FTARWCSTCQVNKASYTDEVLAAFKKYGIVMMKADKTRTNPAIDQELKNLGRTAVPVN
                     ALYLPGRNPVVTRELLSPAYLLEFLEKEMER"
     gene            12731..13138
                     /locus_tag="PK077_01620"
     CDS             12731..13138
                     /locus_tag="PK077_01620"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRN23033.1"
                     /translation="MVKTKTTYEGGLHCSMVHEPSGATLSTDAPVDNNGKGESFSPTD
                     LVGAALAGCMSTIMGIVAERKGIHLEGMTVEVAKHMNADPRRIGKLEVVITVPLPSDY
                     PDRKLLEQAALSCPVKHSLHPDIEIPITWKWVG"
     gene            13291..13731
                     /locus_tag="PK077_01625"
     CDS             13291..13731
                     /locus_tag="PK077_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23034.1"
                     /translation="MLLIPVTVCGLLSGCGGGSGKSGDDGNTSLSGQQVSLFEGGSIL
                     FQLQFSGQDVDVIRMDTRAVYDGVYTYRFNSGENAGVLNIAPAANSSSACTMANVNIA
                     FDDAGRNAGVITSGTITETGLDLDPAIDGVPRPMAGWTCRVHRD"
     gene            complement(13743..14210)
                     /locus_tag="PK077_01630"
     CDS             complement(13743..14210)
                     /locus_tag="PK077_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HRN23035.1"
                     /translation="MNNNSSSTRLPIPQYVMTLAHAAALRSEDPYRKVGAAALDADNR
                     VIGTAYNGLYPGFKAQDTFWASREERQKYMLHAEINLCSLFRRGEAKVVACTTMPCTS
                     CMQALCAHGVKTIYYCEPYDKSEAPAIASLYGVELIQVTDYPLSRHFPLVLDS"
     gene            complement(14200..16812)
                     /locus_tag="PK077_01635"
     CDS             complement(14200..16812)
                     /locus_tag="PK077_01635"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN23036.1"
                     /translation="MPSKKEWRGFYSLILIQAQNAFNEKAAQFLLIPLGVWLASTNAA
                     YGPDSWVNSLQYILACIFVLPYILFSPFVGWLADCFCKARIIQFMSFLQILVLGAMLL
                     CYKYENIEMAVFWFCVFSVQATILSPAKKGVVKDMVGSRQLGYASGLMEMSLILSMLA
                     AQIGIFAWFDILQVSSNDGWEAAAFPTFILTCIAVPVAIASLYLPRYPANQTRKFEWK
                     LFYEHFVQLKYLWSQRDLRLSEIGVSYFWFLAGALMLISLQIAQEHPIDGTGFSMSAA
                     ILMAWLSGGTVVGGVIASIICRKKIELGLIPLGAIGFTIGCIFMSFFAPGSLPSNIGF
                     GITGAFAAAYLVPLNAHLQDNCDPSNRSTVIAAGNLMDCLMGLVAVGFQLMLRNIFSI
                     QNQFWVLAALGVVITIVAFRLIPREFIRMMGLWIMRIVYRSRIIHQDRIPEDGGAVIV
                     SNHVTYGDALFLSLICPRPIRFIVAEEFVAIRWLGWILELFNCLPISSRNPRESLSKA
                     IQALKAGEVICIFPEGQLTRTGTLCAARRGLEMLAKKSRCPIVPIYMDELWGSIFSYS
                     GNRFFSKAPLRVPYRFTAAIGEPIEPDAVNPRMVINTLRELSSTCLEIAASIGRDAIL
                     NHLERIAHKPLVFSKNARLSGYEIAECLMNDTVEAEHPELRKWLSTLLDSTRSQSRLC
                     DFWMNAQQLERVNSLQPRELLLTSVGREEVHETVAAVLWPILTSTPVYLISDGDHSMP
                     EGIRQIAGSDFLRRRLYSLVPNTRTPFYDFSGSGDLVLPNIGWRPCFATDRGIILAMS
                     MKRSVFKLDDGTVQLGMRARTRGRLLPGFYLNPPLSNVIAGATLSTPYSLPPNLYLDE
                     SGFLAELQSSNHEQ"
BASE COUNT         3406 a         4479 c         5566 g         3547 t
ORIGIN      
        1 gggtttttcc cgggagccct gatcttccgg atcgtagaaa tgctccacga tgttttcatg
       61 ttccggcagg tcgcgcaggg attccctgcc ggcggtgccg ttccgggggg cgccttcatt
      121 gtcggagctg ggtgccatgg tgcggaggtg ggggaggtta cggaagtctg gggaatttgg
      181 aaaaatccgg agcgcgtttt tcaacgaagg cgtttttgcc ttccttcgct tcctcgctca
      241 tataatagag cagggtggcg ttgccggcca ggtccagcag gcccatctgc ccgtcgcagt
      301 cggcgttcag ggatgccttc aggcagcgca gggccagagg gctgtggcgg agcatctggc
      361 ggcaccagga gagcgtttcc tcctccaggt cctccagggg aaccacggtg ttgaccaggc
      421 ccatgtccag cgcctgccgg gcatcgtact gcctgcacag gtaccagatt tcccgcgctt
      481 ttttctggcc cacgatgcgc gccaggtagg aggagccgag gcctccgtca aaagagccca
      541 ctttgggccc tgtctgtccg aatttggcgt tgtccgcggc aatggtgagg tcgcagacga
      601 cgtggaggac gtgacctccg ccgatggcgt atccggccac catggcgacg acgggcttgg
      661 gcatggtgcg gatggtgcgc tgcacgtcca gaatgttcag gcgcggcacg ccgtcttcac
      721 cgatgtagcc ggcatttccg cgcactttct ggtcgcctcc ggagcagaaa gcgtccgggc
      781 cttctccggt caggatgacc acgcccaccc tggggtcttc atgggcggcg ttcagggcgt
      841 tgagcatttc atgcacggtc aggggacgga acgcattgcg gacgtggggg cggttgatcg
      901 tgatttttgc gatgctgccg ttgtccgttg tttcatactt gatgtccgtg tattccctgg
      961 cggtggtcca ttgtgcgctc atacggatta ctttaataag gcgcccggca atgggaagcc
     1021 taaaatgcgc cgccttccgg gtatgtccgg aacctgcggg ggatggggcg gtgctgcggc
     1081 cgcaggcgtt ttccccgttg tttccggtgt ttttccggca ggctccccgg tggccggcag
     1141 ctggcggctt ttgtcattta cggcctgcgc ttctgtgcta cattgggaac ggtatgatac
     1201 atgacctact ggtgagcggc gtgacggtgt ttcccgggag agagcatttt gagtttgttc
     1261 cgggaatcaa tgtggtggtg ggaggcaatg attccggcaa aagccatttg ctgaaattat
     1321 gctataccgt ggccaagtgg agcgctgacg gggggagaaa atccctgccg gaaaaatggg
     1381 cggaggagca gcggctcaga aaagacctga tgcgggtgtt cgcgtcccgc gggctggccg
     1441 ggctgacggc ccggaaccgg ggaaacgccc atgcgcgcgt ggaggcctcc atggaggggg
     1501 acggcgtgcc ggagggcatg gggaacctgg tgtttgattt ccaggcgggg catgaagagg
     1561 aggggctgag catccgggaa atgccccggc gttttctgaa cgtgccggtg gtgttcctgg
     1621 cggcgcggga ggtgctgacc atttatccga gttttgtgca ggtgggaagc agattccccg
     1681 aatttctgga cggcggcagt tgggacctct gccgttatct ggatatggag gcggaagcgg
     1741 agcccatcag tacggatgcc gggcgcgtgg ttgctaggct ggaaaaaatc atcgggggga
     1801 aggtggtgaa gcgtgacggg cggttctttt tgcagcgtcc cggacagcag ccgatagaaa
     1861 tgagcctggt ggcggaagga ttcaaaaggc tgggaacgct gagctacctg atccggaacg
     1921 gttccgtgaa aaaaggttcc gtcctgtttt gggatgaacc ggaaatgaat ctgaacgcct
     1981 cccacctgcc cgtgctggtg aaaaccctga cggggcttgc caagacgggg gtgcaggtta
     2041 ttttgtcctc ccacagcctg ttcctgctgc gggagctgat gattcagctt tccgnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnccat ggtgcagaga aagtttttcg ggatgagcgt
     2161 gccgcggggg gaacgttccg gggtaagggt gtcctggggg gaatccctgg aggacgtggg
     2221 gcctatcgag tctcttgaag cggaaatcga acaggcggac aggtatttga agctgagtta
     2281 tgagtcataa gccgtcaaaa gcttattacg tggagggcgt ccaccgtttc tggatcgatg
     2341 aaggctacgt ggtgtgccgc attgaatgtt cggattatta tgcccggcag gcgcagaatt
     2401 tctgcggggg atgcaaggaa atggattttc tgctgtatca tccggaacgc cgggatctgt
     2461 ggctggtgga agtgaaggat taccgttttg atgcccggcc caaggtgggg gaactggtct
     2521 gcgccctgag ccgcaaggtc cgggatacga tgttcctcct ccgggccgct tccgtggccg
     2581 ctccggacga ggagccggcg gagggcattt ccctgaggga tttttccatt caggcctcgg
     2641 gagcggagac gctgcatctg gcctttctgc tggaaatggg tatgggcggc gtctggacgg
     2701 atggcggcct gctggcgaat gtcaagaacc tgctggtggg gtccatgcgt tttctggatg
     2761 cggaactggt ttgcgcgccc attacctatc cccgccgcat cggcccctgg cgcgtgacct
     2821 ccgcccacgg ggaggtgagc aagcgggtgg aaatccgccg cgccagaaaa ttggcggaaa
     2881 tccaggagca gaggcggcgg gagcggctga acgcgcagga ggagggggcc tggcaggaaa
     2941 agagccggga agatgccggt tcccatgcgg ccccggcgga agaaaggatg ccccagtgga
     3001 agcggcgtct ccggcagcgc atgaacggag aaagcgcgaa ccatgagggg cggcgcagaa
     3061 aaagaaagcg ggaatgaggc ctgcgccggt taaggcctta ttgccgggaa gagccccggc
     3121 ccggaacggt gcgggcccgt cctgcggcgg ctgtatggca tgtctcggaa aaaagtttta
     3181 ttttacaagg actcgcgtat catgccgccc tattcattaa cttctgtgtg ctaaccacat
     3241 tatgtctgac agaatgccac ccagtcctcc ccgtcctccc aaatttcccg gctccggcag
     3301 gcccgaatcc cccaactggg gcgtgtgggt catggtactt ctcattgtag gcgtccttgc
     3361 gtttggcttt ttcacccctg aatctttcgg gttgggcccc cgcaaggaaa atctggaatc
     3421 ctttgaagcc cagtataagg cggggcgcgt ggtgctgaac gaccccaagg cccccgtgga
     3481 agtggtgctg agcgaaaacg gttccgaagg cgttattcac gcgctggtgt acaggaagga
     3541 aattcagccg aaggtggaaa tgaccccctt tgcgctgact tattccatgt cccttccgga
     3601 ccgcgacaag cctttgctga atgaactttc cggctacagg gtggtggaga gcccgtaccg
     3661 gacggaagag ggaaagaatg tttccctcat tcctgaggga gcccagaagc tttccgtgcc
     3721 tgaattcaac cgcctggccc tggaaggccg cattgccgga ggaaaggacg gcatcatcct
     3781 ggcggaagac ggcaaccaga acgtgctggt cggacagatt gtcacccgca tctggcccgc
     3841 ggctacggga gacgcctccg tggacaagca gcgttttgaa cgcgtggaag tgccttttac
     3901 cctggagttc cagggagacc gcgtcaagca gcttctgggg ccggatacga agttcaagcg
     3961 tgaatccggt tcctggggcg gcatcctgct gaatctgctg cccatcgtgc tcattctggt
     4021 gattctgttc ttcatgttcc gcgcgcagag cggcggggcc cggggggcca tgggcttcgg
     4081 caaaagccgg gcgcgcctca tttccccgga caaaaacaag gtgacgttca aggatgtggc
     4141 cggcatcagc gaagccaagg aagaagtgtg ggaactggtg gagttcctgc gcaatccgga
     4201 aaaattccgc gacctgggcg ctaccattcc ccgcggcgtg ctgatggtcg gggcgcccgg
     4261 cacgggtaaa actctgttgg cgcgtgccat tgccggggag tccaacgctt ccttctattc
     4321 catcagcggc tcggactttg tggaaatgtt cgtgggggtg ggtgcaagcc gcgtccgcga
     4381 tatgtttgaa caggccaaaa ggacggctcc cagcctgatt ttcattgatg aaattgacgc
     4441 cgtgggccgc cagcgcggtt acggcatggg cggcggcaat gacgagcggg aacaaacgct
     4501 gaacgccctg ctggtggaga tggacggttt tgaaaacaac tccaatgtaa tcgtcattgc
     4561 cgccaccaac cgtgcggaca ttctggatcc ggccctgctg cgtcccggcc gcttcgaccg
     4621 gcaggtggtg gtgaatctgc cggacgtccg ggggcgcgaa cagatcctgc aggtgcatgc
     4681 cagaaaagtg aagatggcgc ccggagtcag ctttgagcgg attgcccgcg gcacgtccgg
     4741 cttttccggt gcccagctgg ccaacctggt caatgaagcc gccctgctgg ccgcccgcaa
     4801 ggggctgaag gagattacgg aggccgaatt ggaagaagcc cgcgacaagg tcagctgggg
     4861 gcgtgaacgc cgcagcctgg cgattaacga acgggggcgc cgcattaccg ccgtgcatga
     4921 ggcggggcat gccatctgcc tgttgaaaac gccgcacagc gagccgctgc accgggtgac
     4981 cattgttccc cgcggcgggg ccctcggcat gaccatgtgg cttccttccg acgacaaaat
     5041 gcaccagctc cggtccgaaa tgctggacca gctcgtcgtg gcgatgggcg ggcgctgcgc
     5101 cgaacaaatc gtttttggcg atgtgaccag cggcgctacc ggagacatca agagcgccac
     5161 caacctggcc cggcgcatgg tgtgcgaatt cggcatgagc gaaaaactgg ggctgattga
     5221 gtacggagaa caccagggag aagtttatat tgcccgcgac ctgggaacgc gttcccgcaa
     5281 ttattccgaa tccacggcgg aactgattga ctcggaagtc cgtttcctgg tggacagcgc
     5341 ttatgaacgc gccatggcca tcctgacgga aaaccgggac aagctggaca tcctgacgga
     5401 agccctgatg gagtttgaaa cgctggaagg ttcccaggtc atggatattc tggaatacgg
     5461 ggaaatgaaa aatcctcccg ccagggtgac tccgcccccc atgccttccg aagtggagga
     5521 acagcctggg aaggatgatt ccggtcatac cgagaagaaa gaaactgaag agacctgcgc
     5581 ggacggcgct gaagaacgga aggaggaaga gaagcaggaa caggcggagc gcgatccttc
     5641 ctcctgcact cctgctgatg aatccggcat ggacggagga gaaaagaaat aattcaatag
     5701 aaacgaatcg aatatggtag ccaatgatgc gatggaactg gccaggatgt gcgcccgcgc
     5761 cgcggatgac gccaaggctg aaaatgtgcg ggtgtacgac ttgcggggca tgtcttccct
     5821 gacggatttc atggtagtgt gcacggggtt gtccgttcct catctgcgcg ccgtcatccg
     5881 ggagctggaa gaagccgtcc gggaaaaggc gggagccctc cccacctatg tggaagatac
     5941 gcctgtggca ttgtggtccg tagtggacta cattgacgtg atggttcaca tcatggggca
     6001 ggaaacgcgc gaattctacg gtatggacac cctgtggaag gatgcccccg tggtggaata
     6061 ttaattactc caatctgact tcacaccgtg ccgcatccgg ttgcggcacg gttttttctt
     6121 ttctggaata aatggcctgt tgtttgccgc gcttcgcaaa aagatggcag tctgcatggc
     6181 cgggttcttc cgcatgaggg cctgtttccg gagaggaaga gaaaagacgc gataatggca
     6241 gaggcaacgc attttctttg cggggctttg gatgattctt ccctggcttt tccttggaaa
     6301 aggggagtgg agccccggtt ctaacaagat tccaaccttg ccttgaagcc gcaatcggat
     6361 tactctttca acgttattta tgagtagttc cggacctgcc tcaaccccga tgatggatca
     6421 gtaccttcgc atgaagaagg gcttgccgga agacgtgctg ctgtttttcc ggctggggga
     6481 tttttacgaa atgttttttg aggacgccaa ggaggcctcc gccattttgg gcctgacgct
     6541 gaccaagcgc cacggcattc ccatgtgcgg ggtgccccac cacagcgcgg aagggtatat
     6601 cggacggctg gtgaagggag gtaagcgggt ggctattgcg gagcagacca ccattccgca
     6661 gcctggcaaa ctggtggagc gggaactgac ccgcgtgatt tccgccggaa ccctggcgga
     6721 catgaatttg ctggattcct cccgccataa ttacattgtg gcgttgtaca gggacaagaa
     6781 gcgcttcggc ctggcatgcg tggaccatac cacgggggaa ttttccgtgg cccagttcga
     6841 acacatggat ttgcttctgg acgagctgtc ccgcatcaat ccctccgaac tcctggtcag
     6901 cgacgagcag acggactgtt tccccggaac ccaccccacg ctttattacg acggatatac
     6961 ttttctgcct tccacggcca ttcccaattt gctgagccat ttccgggtgc attccctgga
     7021 cggcttcggc tgcggggaga tgaccgccgc cctgggtgcg tccggcgcgg tgctccatta
     7081 tctgagctac cagctccgcc gtcccacgga ccacttgcgc cgcatttccg tgcgcgccat
     7141 ggagaacgcc gtgctgattg accaggccag ccagaggaat ctggatctgg tggattcccg
     7201 cggcggcgtg aagctttccc tgctggggac cctggacagg acaagcaccc ccatgggcgc
     7261 gcgcaaactc cgggactggc tgctccaccc actgtgcgat ctggaaaagc ttctggcgag
     7321 gcaggaggtg atcgccgttc tgctgcagga gccctacctg atgagcaagc tgagggagag
     7381 cctgaagaat gtgcgggaca tggagcggct gacggggcgc atttcccagg gtgccgggaa
     7441 tgcccgcgac cttcaggcgc tggcttcttc cctgtcgcgc atccccgcgc tcagggatga
     7501 tttggaatcc ctgcccggcg gcggggagat gctggagagc atccgttccc gcatgggctg
     7561 ttttgatgag ctggtggatt tgctccagcg cgccctggtg gaggaaccgc ccgtgaccat
     7621 caaagagggc ggcatcatca gggaagggta tcacgccggt ctggatgaat tgcggctggc
     7681 ttcccgcgac gggaaggagt ggctggcacg gctgcaggag aaggagcgca agcgcacggg
     7741 aattgattcc ctgaaaattc gctttaataa cgtcttcggt tattacattg aagtaacgaa
     7801 gagccattac gataaagtgc ccccggatta ccagcgcaag cagacgctgg tgaacgcgga
     7861 gcgctttatt accccggagc tcaagcagat ggagaatacc atcctggggg cggacgagcg
     7921 ttcccgccag gtggagtatg agcagttcct cctgttgcgc gaggaagtgg ggcgccacat
     7981 tgacgatatc cagatgacgg cggacgccat ggcggatctg gatgtgctgc tggggctggc
     8041 ggagggcgcg cagcagtacc ggtactgccg ccccgttctg gacaattcca tgaccctgcg
     8101 cattgtcaat ggccgccatc ccgttattga gcagaatgtt tccggcgatg tgttcgttcc
     8161 caacgacgcc tttctggaac cggaggaaaa ccgccttatt ctgctgaccg ggcccaatat
     8221 ggcgggcaag agcacttata tccgccaggt ggccctgatt acgctgatgg cccagattgg
     8281 agcgtatgtg ccggcggaag ccgcccacat cggtttggtg gaccgcattt tctgccgcgt
     8341 gggagccagc gacgacctgg cgcgcggcca gtccaccttt atggtggaga tgagcgaaac
     8401 ttccctgatt ctgaataacg ctacggagcg ctccctgatt attctggatg aaatcgggcg
     8461 gggcacggcc acctttgacg gtctttccat cgcctgggcc gtggcggaat acctgcatga
     8521 cgagctgaag tcccgcaccc tgttcgctac gcattatcat gagctgacgg atttggccaa
     8581 ttccaggcag ggcgtgcaga attacaatgt ggccgtgcgg gagtggaagg aggaaatcgt
     8641 gtttctgcgc aagattgtgc cgggagcggc ggacaagtcc tacggcattc aggtggcccg
     8701 actggccggc atgcctgccg tcattgtgga ccgcgccaag gccatcctgt cccatctgga
     8761 gatgaattcc acgcgcccca ggaaaaaggg gcgctcccgg ctggcggaac cgagagccaa
     8821 gaatacggac atggacgatg atatgcctgt cggggaatat gctcagctgg agttgttctg
     8881 atatgcctcc gctttcggat acaatcggcg gcctgcgttt cgccgccatt gattttgaat
     8941 ccgccggcgc ggcccggggg gagacggacc agcccgtcca gataggcata gccgcatgtt
     9001 cccgcctgga ggacgaacct gagttgtgga cttcctatat tgcggtggag aagccggttt
     9061 tatggtccgc ctcccaggtg cacggcatta ctacggagat gctggcggat gccccgtctt
     9121 ttccttccct gtggccggag atacgttccc ggttggggca ggccgtggtg gtggggcata
     9181 atccggctac ggaacggaag ttcctgcggc ggtttccagg ccacggcttc ggtccgtggc
     9241 tggatacgct ggctttgggc aggatgtgcg tccccggtct ggaggattat tccctgtctt
     9301 ctttgtgcgg cgccctgggg ttgacctgtt ccgtggaggc gttgcttcct gcgcgccgct
     9361 ggcatgacgc tttgtatgat gcgctggcaa gcctgctgat tgtccgtttt ctggtaaagg
     9421 aattgaattt gtccggccag cccctggagg tattgggcaa ggcggtgaga acatgatttt
     9481 cccggcaggg gaaagaatgc ttttccgggc cgcgtattcg gaaaaagatg aatgcttctg
     9541 ttttctctgc cgttttttta ttgctctcct ttgcttcttc ttccgtgtat gcggctcctg
     9601 ctgatgaggc tccggatccg cccgatgttc ccaaactgaa tacccagccg tgggttccgg
     9661 cccgttacag ggcggttcgg gaaaatttgc tggggaagga atgcggcatt ctgttcgtgg
     9721 gggattccat tacggaatgc tgggaacggg aaggtgccgc gctgtggaag aggctgtttc
     9781 tgccgatgaa ggcggtcaac ttcggagtga gcggggacag gacggacagc atgctgtggc
     9841 gtatggagga tacgaaactg gcggtcaaga cttccccccg ctattgcgtt ttactggccg
     9901 ggacgaataa tatcggccgc tggaagggga ggcaagccgc ggcgcagacg gtgaagggca
     9961 tccgggagat agccgtacgc ctgttgaaga agtttccgga gacccgtttg atcctgatgg
    10021 aggtgactcc ctacggctcc gatcccggca gcccgttgcg caggaggcag gaggagatta
    10081 accgtttact gcgcaggctt gagcttccga ggaccactat tttgtccatt aacggtagcc
    10141 tgctgaagcc ggacgggacg ttcagggagg gcatgttcag ggatcatgtc catttaacgg
    10201 agaagggtta tcaggcatgg gcggacgcgt tgctgccgct gttgaacggg gaagggtaag
    10261 ttttctccgt ttcctgctgc ccggcatggc ctggggggag ggcggcggat tgccgcccgc
    10321 atgacaagac gtcctagcgt tccatttcct tttccaggaa ttccagcagg taggccgggg
    10381 acaggagttc cctggtgacg acggggtttc tgccgggaag atacagggca ttgacgggaa
    10441 cggccgtgcg ccccaggttt ttaagttcct ggtcaatggc ggggttcgtg cgggtcttgt
    10501 ccgccttcat catgacgatg ccatattttt tgaaagcggc cagcacttca tccgtatagg
    10561 aggccttgtt gacctggcag gtggagcacc agcgggccgt aaagtccaca taaacgggtt
    10621 tccctccgtc cagggcggcc tgcatggctt ccgggctcca gggtatccag atgtttcctt
    10681 cctcctggag ggagggcgct gcggcagcgg aggactccgc cgccccggga gcggaaccgc
    10741 ggccgaacca ggctgctcct tccggcggaa gcatgtagta caagccggcc agcaggaaga
    10801 ttacggagaa gatgcctccc gtgatgcggg actttctgct gcggtacatg gggcaccatc
    10861 tgccgtatac ccaaaaggcc atggaaaaca gcaccagccc gaagaagagg aacaggatgt
    10921 cctgaggatg gttttcagca tcaaagaagg ctatgtaaat ccagaggaag tagacggcgg
    10981 tgccgaacat caggaaggac atggcctgct tgaaggattc catccatgcg ccgggtttgg
    11041 gcaggtatct ggtcaggacg ggaaaggcgc ccagaatgat gtagggaagg gacattccaa
    11101 gccccatcat ggtcaggcag agtacgatgc ccagaggcgg ctggagcatg gccgcgccga
    11161 tagcctggcc caggaacgga gcgctgcacg gggtggagat gaccgtagcc agggcaccgg
    11221 accaaaagga gccggcataa cctttccggt gctggaggtc gttgcccatg gttgtggctt
    11281 tgacgcctat ttcaaagacg ccgaacatgc tcagccccat ggcgagcatc aggcccagca
    11341 gacagaaatt cacccagggg ttttcaaacc agaacgccca gttgacaacg ccctccgcgc
    11401 cgcggcccag ccagaagtct ccgctgaata cgccggggcc ggctggggcg ctccagtcaa
    11461 acatgttcgc tttcagcgca atcagaatgg cagtaatgat ccagaaagaa atcaggatgc
    11521 ccagtacgaa ggtaagggag tgggccagga cttttttccg ttccccccct cccaactgga
    11581 cgaatcccat aatcttgagg ccgattaccg ggaagacaca cggcatgaca ttgaggatga
    11641 tgccgccgat gaacatgaag aacatgattt cccccaggcc catcagcgga ggagcgggga
    11701 tggaagcgga agattccggg gcggattccg ttccggcggc tgttgcctgg gattccccgg
    11761 agaaggggcg gtcgtccgcg gtgatgatga tgccttccct gccggcgcgc aggatgccgg
    11821 aaaggctggt cagaggtttc cctttgtccg cgtccggcag gttgttggga taaaggccgt
    11881 ccgtggtgtc attgaattgc atggacagct cataattgga gtcatccagt tttttgaagg
    11941 tttggggagt ggtagggttg atttccccct gattgcagaa aaaatagacg gaatcctgcg
    12001 gaacggggcg tccgtttgtc ctgaggtgca gggtgacggt tttcccttcc tgggaaatcc
    12061 gggctttcag cccttccgcc cagtcggggg cggcaagccc tgccatgttc ccggtcaatt
    12121 ccgccgcgtc aggggctgtc tgcccgtctc cgcgcttcag tgtgacgctg aagtttctgg
    12181 tttccggggc ggcgcactgt tccgcgcaca tctgccacgt catggtggtg gtgaacatcg
    12241 cttccggggg ggcatccttt tccggagtta cccggaacgc cattttagcc ttcccgctgt
    12301 atccgtaaaa atccacaagc cctttcccca gttccgggat ttgccagaag gggccttcca
    12361 cccggaagcc gggcacaggc tggaaggcgg cttccatggg aagtcccacg gttcccggat
    12421 ttttgtagta aacgtgccag tggtccggca gcgccagctc cgtcacgatg acgaaggagg
    12481 cttctccata ggaggtgaca gtggccttgg aggaaccgga tgtctggggc ggaccaaagt
    12541 ttcccgcttc cccgaagctc attcctccga agtcctgggc ctcccccgtg ctgaaacacc
    12601 cggcgatcat gcccaggcag ccgacggctg ctttcaaaaa gtttctcata gcaaaggcgg
    12661 ttaccataaa gaacgggggg attcaatgtt tgtgtgttgc atggcgggga gccttgtata
    12721 aaataaggcc atggtaaaaa ctaaaacgac gtacgagggc ggcctccatt gttccatggt
    12781 tcatgaacct tccggggcca cgctttccac ggatgccccc gtggacaaca acggcaaggg
    12841 tgagtccttt tccccaacgg acctggtagg agcggcgctt gccggctgca tgagcacgat
    12901 catgggtatt gttgccgaac gtaaggggat tcatctggaa ggcatgacgg tggaagtggc
    12961 caagcatatg aatgcggatc cccgccgtat cggcaagctg gaagtcgtca ttaccgttcc
    13021 tcttccgtcc gattatccgg acaggaagct gctggaacag gctgctctga gctgtccggt
    13081 caagcacagc ctgcaccctg atattgaaat acccattacc tggaaatggg ttggctgatc
    13141 gcttccgtgt tttcttgaga ccgcctgtga aaacaggcgg ttttttttgt gatgttaaaa
    13201 atgttggaaa aatgaaataa tagttgagta tacttgactt atagttttat gttgtgcatt
    13261 tttccgggga tgaaaaagtt gttccctgtc ttgttgctga ttcctgtgac ggtgtgcgga
    13321 ttgttgtccg gatgcggagg agggtcggga aagtctggag atgacgggaa cacttccctg
    13381 agcggacagc aggtaagcct gtttgaggga ggaagcatct tattccagct ccagttcagc
    13441 gggcaggacg tggatgttat cagaatggat acaagagccg tgtacgacgg ggtttatacg
    13501 taccggttta atagcgggga gaatgccggc gtattgaata ttgctccggc tgccaacagc
    13561 agctccgcct gtaccatggc gaatgtgaac attgcctttg acgacgccgg gcggaatgcg
    13621 ggcgtcatta cttcgggcac cattacggaa accgggcttg atttggatcc tgccattgac
    13681 ggcgttcccc gccccatggc cggttggaca tgccgtgtgc accgggattg attttccggg
    13741 catcagctgt ccaggacaag ggggaagtgt cggctgagcg gatagtccgt aacctggatc
    13801 agttccacgc cgtaaaggct ggcgatggca ggagcttcgg atttgtcata aggctcacag
    13861 taataaatgg tctttacgcc atgggcgcac agcgcctgca tgcatgaagt acagggcatc
    13921 gtagtacatg ccaccacctt ggcttccccc ctccggaaca ggctgcacag gttgatttcc
    13981 gcatgcagca tgtatttctg gcgttcttcc cggctggccc agaaggtgtc ctgagccttg
    14041 aatccgggat acagtccgtt gtaagcggtg ccgatgacgc ggttgtccgc atccagggcg
    14101 gcggccccca ccttgcggta cgggtcctcc gaccgcaggg ctgccgcgtg cgccagcgtc
    14161 attacatact gcgggatggg aaggcgggtt gacgaggagt tattgttcat ggttggaaga
    14221 ctggagttcc gccaggaagc ccgactcgtc cagatacagg ttgggcggca gggaataagg
    14281 ggtggagagg gtagccccgg cgatgacgtt ggaaagaggg ggattcaggt aaaagccggg
    14341 gaggaggcga cctcttgtcc tggcccgcat gccgagctgc acggtgccgt catccagctt
    14401 gaagacgctt ctcttcatgg acatggccag gataatgccg cggtccgtgg cgaagcaggg
    14461 ccgccagccg atgttgggga gaaccaggtc gcccgagccg ctgaagtcat aaaacggcgt
    14521 gcgcgtattc ggaaccaggc tgtagagcct gcggcgcagg aaatcggaac cggctatctg
    14581 cctgatgcct tccggcatgc tgtggtcccc gtcgctgatg aggtaaacgg gagtgctggt
    14641 gaggatgggc cacaggacgg cggccaccgt ttcgtggact tcttcgcgtc ctacgctggt
    14701 gagcagcagt tcccggggct ggagggagtt cacccgttcc agctgctggg cgttcatcca
    14761 gaagtcgcac aggcggcttt gggagcgggt gctgtccagc agggtggaca gccatttcct
    14821 gagttccgga tgttctgctt ccacggtgtc gttcatcagg cattccgcta tttcataccc
    14881 ggagagccgc gcgtttttag agaagaccag cggcttgtgg gcgatgcgct ccagatggtt
    14941 caggatggca tccctgccga tgctggccgc aatttctagg catgtggagg acagctcccg
    15001 cagcgtgttg atgaccatgc ggggattgac ggcgtcaggc tcaatgggct ctccgatagc
    15061 ggcggtgaaa cggtagggaa cgcggagagg cgccttggag aagaagcggt tgcctgaata
    15121 ggaaaagatg ctgccccata gttcgtccat gtaaatgggc acgatggggc agcgcgattt
    15181 tttcgccagc atttccagcc cccgccgcgc ggcgcaaagg gtgcccgtgc gggtaagctg
    15241 gccttccggg aagatgcaga tgacttctcc ggccttcagg gcctgaatgg ccttggacag
    15301 ggattcccgc ggattacggg aggaaatggg caggcagttg aagagttcca gaatccagcc
    15361 gagccagcgg atggctacga attcctccgc cacgatgaac cgtatggggc gcgggcagat
    15421 gagagaaagg aacagcgcat ccccgtaggt gacatggttg gagacgataa cggccccgcc
    15481 gtcttccggg atgcggtcct ggtggatgat gcgggagcgg tagacaatcc tcatgatcca
    15541 gaggcccatc atgcggatga attcacgggg aataagccgg aaggccacaa tggtaatgac
    15601 cacgcccagg gcggccagca cccagaattg gttctggatg gagaagatat tcctgagcat
    15661 gagctggaag ccgacggcca ccagccccat caggcagtcc atcaggttgc cggcggcgat
    15721 gacggtgctg cggttggagg ggtcgcagtt gtcctggagg tgggcgttta acggcaccag
    15781 gtatgcggcg gcgaaagccc cggtgatgcc gaacccgata ttgctgggaa gggagccggg
    15841 agcgaagaag gacatgaata tgcacccaat agtgaagccg atcgcgccca ggggaatgag
    15901 ccccagttca atctttttac ggcagatgat ggaggcgatg acgccgccta ccaccgtgcc
    15961 gccgctgagc catgccatga gaatggccgc ggacatgctg aatccggtgc cgtcaatggg
    16021 gtgttcctgg gcgatttgga gggagatgag catcagggcc ccggccagga accagaagta
    16081 ggaaacgccg atttcactga gcctcaagtc ccgctggctc cagaggtatt taagctgcac
    16141 gaaatgttcg taaaagagct tccattcgaa tttcctggtc tggttggccg gataccgcgg
    16201 gaggtaaagg gacgcaatgg ctacgggaac ggcgatgcag gtgaggatga atgtggggaa
    16261 agcggctgct tcccagccgt cgttggagga gacttgcagg atgtcgaacc aggcgaagat
    16321 gcctatctgg gccgccagca tggacaggat gaggctcatt tccatcagcc ctgaagcgta
    16381 gcccagctgc cgggagccta ccatgtcctt gaccactccc tttttggccg ggctcaggat
    16441 ggtcgcctgg acggagaaga cgcagaacca gaaaacggcc atttcaatgt tttcgtattt
    16501 gtagcacaga agcatggctc ccagcaccag gatctggagg aaagacatga attgaatgat
    16561 gcgggccttg cagaagcagt ccgccagcca tccgacgaaa ggggagaaca ggatgtaggg
    16621 cagaacgaag atgcaggcca gaatgtactg gagggagttg acccaggaat ccggcccgta
    16681 tgcggcgttg gtggaagcca gccagacgcc cagcggaatc agcaggaact gggcggcttt
    16741 ttcattgaaa gcgttctggg cttgaatgag aatcagggaa taaaaacccc tccattcctt
    16801 ttttgagggc atttttttct tagatgtctg tacgtcatcc atgtggggcg gattgtagca
    16861 gatcatgggg tgtagggaag gataatctgt tcccgtacag gcgcgttccc gggaggaggg
    16921 ggaaccgttt tttccggttt tttgaaaggg gcgggcaggg gatggcccgg aggctggcgc
    16981 ggcatgtccg ggcagctttg cccgcgtgcg ggagcggaag ccggagattc
//
LOCUS       DAOMSU010000018        16196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_126_length_16196_cov_2.368255, whole genome shotgun sequence.
ACCESSION   DAOMSU010000018 DAOMSU010000000
VERSION     DAOMSU010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 16196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16196
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126_length_16196_cov_2.368255"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="PK077_01640"
     CDS             complement(<1..1388)
                     /locus_tag="PK077_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23037.1"
                     /translation="MSSERHFSSKPYRPARKKKSYWKPVLFLLLLGGLWFGYIQYWPT
                     IEGWFKPTIHEVHSQTQAMGHLQDMLASWNGSDAELAQMAASVDKQVEWMNSPEARDS
                     FAWLLAVEMDRRGMLKESEPMLAALLGKKLAASSSMTAEDNTRLLGVSLNWAREFARR
                     KHDAVAEKLYEVILKNTPEDQVSIRLACLEPLIHYAYDQARFERFSALCKQAVSPVMR
                     SMLNKPEDVKSMVKILLLQDTLPDRTTGLPGGTGSAIARELLTKFRLTANPDMGRIIL
                     NELKMPLNTRRKYSQEELKAMADQLEAALICFRAAETEMDCTPETMLALARVRMQMGD
                     LREASRLLSRAEGAAMTLGVDAPRILSGSSLSQDIASMRSQLEKYSQAEALVKRAYED
                     VNVAAAFLKAQDYDQAVKYLEQAMKVARENTTFVQALQPVILNLQAEISAGREQWALS
                     ESQYGKLIAEWDA"
     gene            complement(1466..3115)
                     /locus_tag="PK077_01645"
     CDS             complement(1466..3115)
                     /locus_tag="PK077_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23038.1"
                     /translation="MPPLSPRTKWLIALCLCVLVMLLEFLTYHMAYRIGYDEGMLTTP
                     PVVVQKSDEKAMQNLSRFMADVFASRESLAGILDNREERLAWIRDPELRTETAWGLTR
                     ELISRGGVVPALPAARELIDAGYAAGRYQVWAPRADAVAKALLAEHQYTSAYDYLKTA
                     EEGYEKEGMAEPLVRTLQTMSSIDQMLNRNEQANMLLQRAVDAAAHLGPDALPVRSRL
                     LAAQGRLARTTGRIPESREYFKKALDLCPQPDKTTGDLALASISMGEAMLEAGRKDEA
                     RELFLKGLSGSENASYLLNDCLNALRGLARISTEQGNYEEALAYLYQAEGAARGVLPA
                     DHAFWAGLYDQRGWVNILRKASPEARADFLKAIANSSASPVISAQSREGLGKAWLDAG
                     EGGEARENLEKALQLRKSHFASDLLSLGRVYYSLGLASDMAGDREGALTAYAGAVDSL
                     LKCVEGAERRDLLVQSYLCKAYALCDGEQWKEAAEAFEAVLPMLEGEQRSENYKQLGR
                     CYDELGMKEKADDCWKESGFPRVRMASPGRRPSVNSRSSRR"
     gene            complement(3351..4247)
                     /locus_tag="PK077_01650"
     CDS             complement(3351..4247)
                     /locus_tag="PK077_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23039.1"
                     /translation="MFKSLIRTFAIVAAFIAGYMMPGLHVYSWTFKWMLVVMLFVTFL
                     GIRFKRMKPERAHWLLTGANLMVALAAWGVCRLVFGDGYLAQAAFFVGIMPTATAAPV
                     VMGLLGGSVEFMLTALLLINGIICTLLPFILPAVVGRGGFEIYLNVAQNISLVMLLPL
                     ALALAARRFYPRAIAWPRKLKDVTFGIWVVILVLIAANASYDISSREGISERVLEQIG
                     VIALLVCGVNFGLGYLLGGRTRAAECSQALGQKNTTLSIYLALTYASPIAALGPTFYV
                     LWHNLWNAWQLYRVSERKRRDG"
     gene            4258..4497
                     /locus_tag="PK077_01655"
     CDS             4258..4497
                     /locus_tag="PK077_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23040.1"
                     /translation="MKRLRGISEQMDETAHPMSVPSRQISQTGRQADYHALSNGPHST
                     PMMPGNAHIAMSGNKEIFCNGQPFPDSLLLHHGEH"
     gene            complement(4512..5810)
                     /gene="hisD"
                     /locus_tag="PK077_01660"
     CDS             complement(4512..5810)
                     /gene="hisD"
                     /locus_tag="PK077_01660"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HRN23041.1"
                     /translation="MKIYRPSDTCFDEMKSRMNRRALPEDSVRDTVNAIIQDVSARGD
                     EALFDYASRFDKVHLDSSSLFVTEAELAEAEAMVEGSVKEAIAVSLANIHYFSDRSRR
                     QDWSGVNAQGVEVAERFLPYDRVGIYIPGGKAPLVSTSIMTGGFAQAAGVREIVAATP
                     CGPDGRVNPSLLYALKASGATEIVKIGGAQAIAALALGTESVKPVEKIFGPGNRFVVE
                     AKRQLVGAVAIDLLPGPSEVMVLADDTADAEFLAADLLAQGEHGPDSVVVFVTTSEAL
                     LEQVDAEVERQAALLSRGSIIREVLDKHAYGFLVSSIQEGVELVNAFAPEHLVLVTRD
                     EEAVLNGIRTAGAIYAGSLSTVACGDFLAGPSHTLPTGGAGKSFSGLRADQFQRRTSV
                     VRMDRDAVLNSAPYVAEFARVEGLDAHNHSIQVRAARVNR"
     gene            6012..7193
                     /gene="sucC"
                     /locus_tag="PK077_01665"
     CDS             6012..7193
                     /gene="sucC"
                     /locus_tag="PK077_01665"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRN23042.1"
                     /translation="MNIHEYQAKQLFERFGVATPKGIAAATAQEAAQTARNMGLSQYV
                     VKAQVHAGGRGKGTFKNGFKGGVHVVKSVEEVEEVAGKMLNQVLVTKQTGETGKLVSK
                     IMVAEAVDLKKECYFAILQDRARECPVIVASTEGGMDIEEVATTRPEAIIREHIDPAL
                     GILPFQALKIAVALGLTGPLLRQTTKLITNVYKLFTALDCSLVEINPLVVTTDDRVCA
                     LDAKFNFDDNALYRHPEIMEMRDETEEDPREVEAGKYDLNYIGLDGNIGCMVNGAGLA
                     MATMDIIKYYGGEPANFLDVGGSATEEMVTNAFRILTSDKNVKALLVNIFGGIMRCDV
                     IAQGIVAAAKNIDMKIPLVVRLEGTNVEIGKKILADSGISIIPADNLDEAAQKAVAAV
                     K"
     gene            7215..8102
                     /gene="sucD"
                     /locus_tag="PK077_01670"
     CDS             7215..8102
                     /gene="sucD"
                     /locus_tag="PK077_01670"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRN23043.1"
                     /translation="MTPLIDKNTRLVVQGITGSAGAFHAKNCMDFGTNVVAGVTPGKG
                     GQLFEGKVPVFDTVAEAKKATDCNASMIFVPPPFAADAIMEAADAGVKLIVCITEGIP
                     VQDMQKVKTFLKDKDVTLIGPNCPGIVNPSANCKIGIMPAYIFKPGRIGIVSRSGTLT
                     YEAVWQVTNMGLGQSMCIGIGGDPVHGMTQQQAVQFFTEDPNTDAFIMIGEIGGSEEE
                     EAAEWIKNNCKKPVAAFIAGATAPKGRRMGHAGAIVAGGKGTAAAKQEALKNAGIVVA
                     KTPAQMGAALAEAMKNKGM"
     gene            complement(8298..9020)
                     /locus_tag="PK077_01675"
     CDS             complement(8298..9020)
                     /locus_tag="PK077_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23044.1"
                     /translation="MNNNIKEHPIQFEADMIRALLQEYSTPGEHKNQTRRTCGLNQFN
                     DLPEALKERGWEIQEFIEESGSWLAVSEEGSGKFSDIFIPWIECPYGKVGDRLWVQEE
                     ICHNSRSGWLYSADGVCLPMSYPDGWISKNARRQFIPSIHMPRWASRILLEITEIRVE
                     RFLDITTNDARMEGIECVWHDEETDACLWRDYSGKSAGLAFARLSFFSLWEKIKGTGS
                     AELNPWVWVIKFNVLTINGNIK"
     gene            complement(9583..9675)
                     /locus_tag="PK077_01680"
     tRNA            complement(9583..9675)
                     /locus_tag="PK077_01680"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9639..9641),aa:Ser,seq:gct)
     gene            complement(10035..12185)
                     /locus_tag="PK077_01685"
     CDS             complement(10035..12185)
                     /locus_tag="PK077_01685"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HRN23045.1"
                     /translation="MSEFKVIRSPMEALKKYFGFSCLREGQDQVVASIMEGRNVLVVM
                     PTGGGKSLCYQLPSLCRAGVCLVVSPLIALMKDQVDALVARGIPATMINSSLSLPEQK
                     ERLDGMKKGAFKLVYVAPERFGHEGFMHALAEVDVNMVAVDEAHCLSQWGHDFRPDYL
                     KLGKAVEMMGRPQVAALTATATPRVREDILKHLGLDDPVTIVRGFARENLYFRITACD
                     THKDKYKRLYELVKRYKTGIIYCSTRKKVEQVYEALSDFGLSVTAYHAGMTDEQREEA
                     QNAFMNRHADIVIATNAFGMGIDRADVRFVAHFEIPGSVEAYYQEAGRAGRDGEEAYC
                     ELLFNHADLRTQEFFIEGVNPGIPLIVELYELLRKHCSAETHDVMWSLEEMAERLKCR
                     NAMQVGAALSVLIRNGVISRHDVPGQRVKLTRVTDPSVSGLKIPLDEDALREKEVRDR
                     EKLKAMTEFAYSSGCRQQWILNYFGEEDGVPCGRCDQCLALGVEEGQSLGEEETLVVR
                     QALSGIARASVRLADGSWQGRWGRTKIIQMLKGAKTQELLRTSLVRLSTYGILSRWSE
                     DDIRQLFRAMQMAGLTRMSGEADRPLITLSPKGNEVMMGRKKASLVWPFARRGKISVS
                     MEQARVRSTGNLAALGEFDEDLFLKLKELRNELAREAGIPAYAVFHNSTLEGLARLKP
                     TTRRGAMNVHGIGEQKAARYLDDFLEVIAEHCGV"
     gene            12399..12475
                     /locus_tag="PK077_01690"
     tRNA            12399..12475
                     /locus_tag="PK077_01690"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12433..12435,aa:Arg,seq:acg)
     gene            12557..12916
                     /gene="ssrA"
                     /locus_tag="PK077_01695"
     tmRNA           12557..12916
                     /gene="ssrA"
                     /locus_tag="PK077_01695"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(13350..13757)
                     /locus_tag="PK077_01700"
     CDS             complement(13350..13757)
                     /locus_tag="PK077_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23046.1"
                     /translation="MREIQIQFSKPGNWREFTLTAIYQDSDGYTRIDRYKQNDIPSGQ
                     APALSAAVAVIADMEEDWQAVQVWARLGNTSVLNNSAGDDEAVEFREAVLLTIEAVNS
                     LGGRRIFAPGNYAQFILMDFASISFFKYFTIRK"
     gene            14416..15339
                     /locus_tag="PK077_01705"
     CDS             14416..15339
                     /locus_tag="PK077_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRN23047.1"
                     /translation="MTTGNTALATTQYDSGIPRNSVPDRTSDNSAKRVIMISLDGICV
                     EGFQKAHAPSLKGMMSQGAFSLDTRVVIPSVTLPNWTSHLTGSGPEQHGVFNNNWTPA
                     NSSYPAVRRDEDGYYPSVFQALKKCQPSCKTAFYYNWKPLAYPFNEKYLDEIKYLAND
                     AYASNYHQAFQFVKKHRYNPTVVFLYSVHTDHAGHKYGWMSREYLQSIEEADQQIGIF
                     FQKLRQEDLFNDTHFLFLTDHGGIGKGHGGTSAAEMIVPWGISGPGIKRNFLISEPNN
                     TTNTAPMLLHLFGGKPLPEWTGKVIESVFPS"
BASE COUNT         4111 a         4787 c         3730 g         3568 t
ORIGIN      
        1 gcatcccact ccgcaatcaa ctttccatat tgggactccg acagagccca ttgttccctg
       61 ccggcgctta tctccgcctg aagattcaga atgaccggct gaagtgcctg aacaaacgtg
      121 gtattttcac gagccacttt cattgcctgt tccagatact ttaccgcctg atcgtaatcc
      181 tgcgccttca ggaaagctgc ggcaacattc acatcttcat aagcccgttt caccaaagcc
      241 tctgcctggc tgtatttttc aagctgggac cgcatggacg caatatcctg gctcaggctg
      301 gagccgctca aaatacgggg agcatccacg ccaagcgtca tggcagcccc ttctgcacgg
      361 gacagcaggc gggaggcttc tctcaaatcc cccatctgca tccttacccg tgccagggca
      421 agcattgtct ccggcgtgca gtccatctcc gtttcagctg cacggaagca gatcagagcc
      481 gcttccagct ggtcggccat ggccttcaat tcctcctggg aatacttccg gcgcgtgttc
      541 agcggcatct tcagttcatt cagaataatg cggcccatgt ccggattggc ggtcaggcgg
      601 aatttagtaa gaagctcccg ggcaatcgca ctccccgtgc caccgggcaa cccggtggtc
      661 ctgtctggca gagtatcctg aagcaacagg attttcacca tgctcttcac atcctccggt
      721 ttattcagca tgctcctcat gacaggagaa acagcctgct tgcacaacgc ggaaaaacgc
      781 tcaaacctgg cctggtcgta cgcataatgg attaatggct ccaagcacgc taaacggatg
      841 gaaacctggt cttccggtgt atttttcaaa atcacttcat aaagcttttc cgccacagca
      901 tcatgcttgc ggcgggcgaa ttcccgcgcc cagttcaggc tgacgcccag aaggcgggta
      961 ttgtcctccg ccgtcatgga ggaggaagca gccagctttt ttcccagaag agcagccaac
     1021 ataggttcgg attctttcag catgccgcgc cggtccattt ccaccgccag aagccatgca
     1081 aaggaatctc tggcttcggg agaattcatc cattccacct gcttgtccac ggaggctgcc
     1141 atctgcgcca gctctgcatc ggaaccgttc catgaagcca gcatatcctg caaatgcccc
     1201 atggcctgcg tctggctgtg cacctcatga atggtcggct tgaaccaccc ttctatcgta
     1261 ggccagtact ggatgtagcc gaaccagagt cctcccagga ggagcaaaaa gaggaccggc
     1321 ttccaataag actttttctt gcgtgcgggg cggtagggct tactggaaaa atggcgctcg
     1381 gaactcatgc aggcattgag ccaaaaagac ggcacaaatc aagcaaaata aacgcctcgc
     1441 aacgtcttac gaagcccctc gcatcctacc tccgtgaaga tctggagttc acggagggtc
     1501 tgcgcccggg ggatgccatg cgcacccggg gaaaaccgga ttccttccag cagtcatccg
     1561 ctttctcctt catgcccagt tcgtcataac agcgtccaag ctgtttgtaa ttctccgacc
     1621 tctgttcccc ctccagcatg ggaagaaccg cctcaaaagc ttctgccgcc tctttccatt
     1681 gttctccgtc acacagagcg tacgccttac acaaataaga ctgaaccagc aaatccctgc
     1741 gttccgcccc ctccacgcat ttcagcagag aatccaccgc tccggcatag gcagtcaggg
     1801 cgccctccct gtcccccgcc atatcgctgg caagtcccag ggaataatat acgcggccca
     1861 gggacagaag atccgaggcg aaatgggact tccgcagctg aagagccttt tccaggttct
     1921 ccctggcctc gcccccctcc ccggcatcca gccatgcctt gcccagccct tcgcgggact
     1981 gggcggaaat gacgggagaa gccgagctgt tggcaatggc ctttaaaaaa tccgcccggg
     2041 cttccggaga ggccttgcgc aagatgttga cccaccccct ctgatcatac aacccggccc
     2101 aaaaggcatg gtccgccggc agtacgccgc gggcagcgcc ttccgcctgg taaagataag
     2161 ccagggcctc ctcataattc ccctgctccg tagaaatacg ggccaggccg cgcagggcat
     2221 tgaggcagtc attcaacagg taagaggcat tttccgagcc ggaaaggccc tttaaaaaca
     2281 attcccgtgc ctcatccttc ctgccggctt ccaacatggc ctcccccatg ctgatgctgg
     2341 ccagggccag gtctccagtg gtcttgtccg gctgaggaca aagatccaga gccttcttga
     2401 aatactcgcg cgattccgga atgcgccccg tagtacgtgc cagccttcct tgggcggcta
     2461 aaagcctgga cctgacaggg agggcatccg gccccagatg tgcagcggca tccaccgcac
     2521 gctggagcaa catattagcc tgttcattcc tgttcagcat ctggtcaatg gaactcatgg
     2581 tctggagcgt ccgcaccagc ggctcagcca ttccttcctt ctcatatccc tcctcagcgg
     2641 ttttcaaata atcgtaggcg gaagtgtact ggtgctccgc caacagagcc tttgccacgg
     2701 catccgcacg gggagcccat acctgatagc gcccggcggc gtatccagcg tcaattaatt
     2761 ccctggccgc aggaagagca ggaacgacgc caccccggct aatcagctcc cgggtgaggc
     2821 cccaggccgt ttctgtccgc agttccggat ccctgatcca tgcaaggcgt tcctcccggt
     2881 tgtccagaat gccggccagg ctctcgcgcg aagcaaaaac atccgccatg aagcgggaca
     2941 aattctgcat ggccttttca tcgcttttct gcaccaccac cggcggagta gtcagcatcc
     3001 cttcatcata gccgatgcgg tacgccatgt ggtacgtcag gaactccagc aacatcacaa
     3061 gcacgcacaa gcacagggca atcagccact tggtacgcgg ggacagaggg ggcaaaggag
     3121 aagacgcctg cataacaagt gcataacgtt accgggcgtt ccccgaattt caagcttttt
     3181 gcacgggaag gaatcctctg cctttcctga aagtggataa aaaagaacct tcaacaatct
     3241 gccagccgtc acttggaacc ggctcaaaag aaccctccct cccgtttttc cgaccatgga
     3301 aatccccata attctgccgg ggacttccgc cgcaccggat gcccccttcc tcatccatcc
     3361 ctgcgctttc gttcggacac gcggtacaac tgccaggcat tccataaatt atgccacagt
     3421 acataaaacg tggggcccag agcggcaatg gggctggcat acgtcaaagc cagataaatg
     3481 gacagggtgg tattcttctg ccctaatgcc tggctgcact ccgcagcgcg cgtgcgtccc
     3541 cccagcaaat atcccaaccc gaaattgacc ccgcaaacca gcagggcaat cacgccaatc
     3601 tgttccaaaa cacgttccga aatgccctca cgggaggaaa tatcataaga ggcgtttgcg
     3661 gctatcaaaa ccagaatcac cacccaaatg ccaaaagtga cgtctttcaa cttcctgggc
     3721 caggcaatgg cgcgcggata aaaacgccgc gccgccagcg ccagcgccag gggaagcaac
     3781 atgaccaagg aaatattctg ggccacgttc agataaatct caaaaccgcc ccgcccaaca
     3841 accgccggca ggatgaaagg cagcaaggtg cagataatac cgttaattag caaaagggcg
     3901 gtcagcataa actccacgct gcctcccagc agccccatca ccacgggagc cgccgtggct
     3961 gtaggcatga tgcctacgaa aaaagcggcc tgtgccagat acccatcccc aaaaacaagg
     4021 cggcacacac cccatgccgc cagggcgacc atcagattag cccccgtcag gagccagtga
     4081 gcccgttccg gcttcattcg tttgaagcgg atgcccagaa aggttacaaa cagcattact
     4141 accagcatcc acttgaatgt ccagctgtac acatgcaggc cgggcatcat gtaccccgcg
     4201 atgaaggccg ccacaatggc aaacgtgcgg atgagggact tgaacatgag gaatacggtg
     4261 aaacgattac gcgggatttc cgagcaaatg gatgaaaccg cccatccgat gagcgttcca
     4321 tcccgacaaa tctcgcaaac gggtcggcaa gccgactacc atgctctcag caacggcccc
     4381 cactccaccc ccatgatgcc gggaaacgcc catatagcca tgtccggcaa caaggagatc
     4441 ttctgcaacg ggcaaccgtt ccctgattcc ctgctgttgc atcatggaga gcattgagga
     4501 aaattccggg attaccggtt cacgcgggcg gcgcgaacct gaatggagtg gttgtgggcg
     4561 tccagcccct ccacccgggc aaactccgcc acatacgggg cggagttcag cacggcatcc
     4621 cggtccatgc gcaccacgct ggtgcggcgc tggaactggt ccgcccgcaa gccggaaaaa
     4681 gacttgccgg cgccgccggt aggcagcgta tggctgggac ccgccagaaa atccccgcag
     4741 gctacagtag aaagggagcc tgcgtaaatg gcgcccgccg tcctgatgcc attaagtacg
     4801 gcctcttcat cccgagtgac aagcaccaaa tgctccggcg caaaggcatt aaccagttcc
     4861 accccttcct gaatggaaga aaccagaaaa ccgtaggcat gcttgtccag cacctcccgg
     4921 atgatggatc cgcggctcag cagggcggcc tggcgttcca cttccgcatc cacctgctcc
     4981 aataacgcct ctgatgtagt gacaaaaaca actacgctgt ccgggccatg ctcgccctgc
     5041 gccagcaggt cggcagccag aaactctgca tccgcagtat catccgccag caccattact
     5101 tcactgggtc cgggcagcaa atcaatggca acagccccca ccaactggcg tttggcctcc
     5161 accacaaaac ggttgccggg accaaaaatc ttttccaccg gtttcacgct ctccgtcccc
     5221 agtgcaaggg cggctatcgc ctgagcgccc cctatcttga caatttccgt cgcgccggag
     5281 gctttcagcg catacaaaag tgagggattc acccggccgt ccggcccgca gggcgtagcg
     5341 gccacaatct cccgcacgcc ggcggcctga gcaaaaccgc ccgtcatgat ggaagtggat
     5401 accaggggcg ctttccctcc gggaatataa atgcccacac ggtcgtacgg aaggaaccgc
     5461 tccgccacct ccacgccctg cgcatttacg ccggaccagt cctgcctgcg gctgcggtct
     5521 gaaaaataat ggatatttgc cagagagacg gcaatggcct ccttcacact cccctccacc
     5581 atggcctccg cttccgccag ctctgcctcc gtcacgaaca gggaggaaga atcaagatgt
     5641 accttgtcaa atctggaggc atagtcaaaa agggcttcat ccccgcgcgc ggaaacatcc
     5701 tggataatag cgttcacggt atcccttacg gaatcctccg ggagggcgcg gcggttcatc
     5761 cggctcttca tttcgtcaaa gcaggtatcg gaaggacggt aaattttcat aaaaagtctc
     5821 tacagaaaaa cgggaaggaa gtttcccaat ctttaagcac gcagtcaagc cttctgtcct
     5881 ctttgatttc caacagggct tcacggcacc tccggacaaa aaatcaaaag accaaaccat
     5941 tctcccttga ccgacatcaa aaatagtcct agcctttctt ccgcaaacat ttggccaaac
     6001 ggcccactgt catgaacatt cacgaatatc aagcaaaaca actctttgag cgttttggcg
     6061 tggctacccc caagggcatt gccgccgcca cagctcagga agcagcacaa acagcccgga
     6121 acatgggcct ctcccaatac gtagttaagg cacaggtaca cgctggcggc cgtggtaaag
     6181 gcaccttcaa aaacggtttt aagggaggcg tccacgtcgt caagtccgtg gaggaagtgg
     6241 aagaagtcgc cggcaagatg cttaaccagg ttctggtcac caagcaaacc ggggaaaccg
     6301 gcaagctggt cagcaaaatc atggtggcgg aagccgtgga tctcaaaaag gaatgctact
     6361 tcgccattct tcaggaccgc gcccgggaat gccccgttat cgtggccagt acggaaggcg
     6421 gcatggacat tgaagaagtg gctaccaccc gcccggaagc cattatccgc gaacacattg
     6481 acccggcgct gggcatcctg cctttccagg ctctcaaaat cgccgtggcg ctgggactga
     6541 ccggtcccct gctccgccag accaccaagc tcatcaccaa cgtttacaag cttttcaccg
     6601 ctctggactg ctctctggtg gaaatcaacc cccttgtagt taccacggat gaccgcgtgt
     6661 gcgctctaga cgccaaattc aattttgacg acaacgccct gtaccgccac ccggaaatca
     6721 tggaaatgag ggatgaaacg gaagaagacc cccgcgaagt ggaagccggc aagtacgacc
     6781 tgaactacat cggcctggac ggcaacatcg gctgcatggt gaacggcgcc ggactggcca
     6841 tggctacgat ggacatcatc aaatactacg gcggcgaacc cgcaaacttc ctagacgtag
     6901 gaggctccgc cacggaagaa atggtaacca acgcgttccg catcctcacc agcgacaaaa
     6961 acgtgaaggc ccttctggtc aacatcttcg gtggcatcat gcgttgcgat gtcattgccc
     7021 agggcattgt ggctgcggcg aaaaacatcg atatgaaaat cccgctcgtc gtacgtctgg
     7081 aaggcaccaa cgtagaaatc ggcaagaaaa tccttgcaga cagcggcatc tccattatcc
     7141 ctgccgacaa cctggacgaa gctgcccaga aagcggttgc ggcagttaaa taaccctcac
     7201 tatttattac gacaatgaca cctctcattg acaaaaacac ccgtctggtc gtgcaaggca
     7261 tcaccggaag cgccggcgcg ttccacgcca aaaactgcat ggacttcggc accaatgtgg
     7321 ttgccggtgt gacccccggc aagggcggcc agctctttga aggcaaagtc cccgtcttcg
     7381 atactgtggc ggaagccaaa aaagctacgg actgcaatgc ctccatgatt tttgttcctc
     7441 cgcccttcgc agcggacgcc atcatggaag ctgctgacgc gggcgttaaa ctcatcgtct
     7501 gcatcacgga aggcattcct gtgcaggaca tgcagaaggt gaaaaccttc ctgaaggaca
     7561 aggacgtaac cctcatcggc ccgaactgcc ccggaatcgt caatccttcc gccaactgca
     7621 agatcggcat catgccggcc tacatcttca aacccggcag aatcggtatt gtctcccgtt
     7681 ccggaacgct tacctatgaa gccgtctggc aggttaccaa catgggcctt ggccagagca
     7741 tgtgcatcgg catcggcggc gaccctgtcc atggcatgac ccagcaacag gccgtgcaat
     7801 tcttcacgga agatccgaac acggacgcct tcatcatgat tggcgaaatc ggcggttctg
     7861 aagaagaaga agctgctgaa tggatcaaga acaactgcaa aaaacctgtt gccgcgttca
     7921 tcgcaggagc cacggcgccc aagggccgcc gcatgggcca tgcaggagcc atcgttgccg
     7981 gaggcaaagg tactgccgcc gctaagcagg aagccttgaa gaacgccggt attgtggttg
     8041 ccaagacacc ggctcagatg ggagccgccc tggcggaagc catgaaaaac aaggggatgt
     8101 aacctccctc tttttcacct tttcatatcc agcgccttcc cttgccggaa ggcgcttttt
     8161 tcatgataaa acggagttga ttacatcacg cggattttat ctgaattgca acggaggcta
     8221 ttccctccgc ttagtgggcg aaccctctgt aaaagacata ttcttatttt gttcttcaca
     8281 aagggcatta acattcttta tttgatattt ccgttgatag ttaacacatt gaacttaata
     8341 acccataccc acggattcag ttcagcggaa ccggttccct ttattttttc ccacaaagag
     8401 aagaaagata accgggcaaa agccaacccc gcagattttc ccgagtaatc cctccataaa
     8461 caggcatccg tttcttcatc gtgccaaaca cattcaatgc cctccatccg tgcatcgttc
     8521 gtcgtaatgt ccaaaaaccg ctcaactctg atttccgtaa tttccagtaa aatccgggaa
     8581 gcccaacgcg gcatgtggat cgaaggaata aactgcctgc gggcattttt tgatatccat
     8641 ccgtctggat atgacattgg caaacaaacg ccatcagcag aataaagcca tccagaacgg
     8701 ctattatggc aaatctcctc ctgcacccac aaacggtcgc caactttccc gtagggacat
     8761 tcaatccacg ggataaaaat atctgaaaac tttccgcttc cttcctcgga aacagccagc
     8821 cacgagccgg actcttctat aaattcctga atctcccacc ctctttcttt caaagcttca
     8881 gggagatcat taaactggtt caggccgcac gtgcggcggg tttgattttt gtgctcgccg
     8941 ggagtgctgt attcctgcag caatgcccga atcatatctg cttcaaattg gataggatgt
     9001 tctttaatat tgttattcat ggtcttgacc attgataaca ccagattatt ccaaaatccg
     9061 ttaaatcctc cgcagattaa aaaaaaacag agcccattct ggagtggctg ttccatactt
     9121 tcccacacat cctgaagctg ttaacatatc cgatgcggcc gtgaaccact ccgagaccgg
     9181 cgcatgaacc acattcgtgt ccattcaatt ccgggcactc tccactttta ccagccacgg
     9241 cagttttatc ctgtccatct cctgcaacaa ctaacttaca ttcaaaaatt tatatcatca
     9301 ttcacacaca cacaaatacc ctcagggaca gcctccagtc cagacctttt tccaaatgca
     9361 cctataatta tcctgatgcc tataataagg atattagaaa aaacgctggc tatgcatcct
     9421 aaccggcgtt tttggcctaa gttattcaat ttcaaatgaa aaaataaaaa actaaaataa
     9481 gacaatcaga ccagcaaata attccccagc atctaatccc ctggtttgca aagaaaaaga
     9541 gcctacaccg ttaaagtgca gactcttaaa aaagaaaggc tttggcggag cgggagggat
     9601 tcgaaccctc ggtaccggtt ttgccagtac gttcctttag caaaggagtg ctttcagcct
     9661 ctcagccacc gctccgtctt gggtaatgtg tggaaaggag actacacgct tcccaaaatg
     9721 aagtcaatac ataaataata cagtcaaaaa tattgatcag gcaatcttct agatcctact
     9781 ggatcgggat agacagccat tcatttgatt gttccatgag ggcaggaaaa caaccttctg
     9841 gaaagagtca aaatatccac aacttgatgt tgatgaacaa tttattcaaa caaatacgca
     9901 ttttcccggc aaatatattc gcaaagcatg gaacattttt cccgtaattc atgaaaaata
     9961 gttcctcagt tcagcatgaa caccacgttc cagagacggc gtacgcctga ttggagcaaa
    10021 tcggaaagac aaattcacac cccgcaatgc tcggcaatga cttccaggaa atcatccaag
    10081 taacgagcag ctttctgctc tccaatgccg tgaacgttca tagcaccacg gcgggtggta
    10141 ggtttcaacc tggccaaacc ttccagtgta gaattatgaa acacggcata agccggaatc
    10201 ccggcttccc gtgccagttc attcctcagc tccttcaact tcaaaaacaa atcctcgtca
    10261 aactctccca gggcagccaa atttcccgta gaccgaactc tggcctgctc catagatacg
    10321 gatatttttc cccggcgggc aaaaggccaa accaaagatg ccttcttgcg ccccatcatc
    10381 acttcatttc ctttcggact cagggtaatc aggggacggt ccgcctcccc gctcattctg
    10441 gtcaatcccg ccatctgcat ggcacggaac aactggcgga tatcgtcctc actccatcgg
    10501 gaaaggatgc cataagtact cagccggacc agggatgttc ttaacaactc ctgggttttc
    10561 gcccctttga gcatctggat gattttcgtc ctgccccacc ttccctgcca ggaaccatca
    10621 gccaaacgca cggatgcgcg cgcaatcccg ctcaacgcct gccgcaccac caaagtctct
    10681 tcttctccca gggattggcc ttcctcaacg cccagggcca ggcactggtc gcagcggccg
    10741 cacggcactc catcttcctc cccgaaataa ttcaaaatcc attgctgacg gcagccggag
    10801 gaataagcga actccgtcat tgccttcagt ttttcccggt ccctcacttc cttttcccgc
    10861 agggcatctt catccagagg aatcttcaag ccgcttacgg aaggatccgt cactctggta
    10921 agcttcacgc gttgtcccgg aacgtcatga cggctgatca ctccattgcg tataagaacg
    10981 gataaagcag ctcccacctg catggcattg cggcatttga gacgctccgc catttcctcc
    11041 agagaccaca tgacatcgtg ggtttccgcg ctgcaatgct tcctgagcaa ctcgtacagc
    11101 tccacaatca atggaattcc cggattcact ccttcaataa aaaattcctg ggtccgcaaa
    11161 tccgcatggt tgaacaaaag ttcgcaataa gcctcctccc cgtctcgtcc ggctcggccg
    11221 gcctcctgat aataggcctc cacactgccg ggaatctcaa aatgggcaac aaacctgacg
    11281 tccgcacggt caatgcccat tccaaaagca ttcgtggcaa taacaatatc cgcatggcgg
    11341 ttcatgaaag cgttctgggc ttcctctctc tgttcatccg tcatgcctgc gtgataggct
    11401 gtcacgctca atccaaaatc ggaaagagcc tcatatacct gctccacttt tttgcgggtg
    11461 gagcaataaa taatacccgt cttgtaacgc ttcaccaatt cgtacagcct cttgtatttg
    11521 tctttgtgcg tatcgcacgc agtaatgcgg aaatacaggt tttcccgcgc aaaccctcgt
    11581 acgatcgtca ccggatcatc cagccccaaa tgtttcagaa tatcttcccg cacccgcgga
    11641 gtagccgtgg cggtcagagc agccacctga ggccgcccca tcatttcaac agcttttccc
    11701 agcttcagat aatccggacg gaaatcgtgc ccccactggc tcaggcaatg cgcttcatcc
    11761 acagccacca tatttacatc cacctccgcc agagcatgca tgaacccttc atgaccaaaa
    11821 cgttcaggag ccacatatac cagtttgaac gcccccttct tcattccgtc caaacgttcc
    11881 ttttgctcag gtaaactcag ggagctgtta atcatggtgg cgggaatccc tctggcgaca
    11941 agagcatcca cctgatcttt cattaaagcg attagaggac tgacaaccag gcaaacccca
    12001 gcccggcaca aggagggaag ctggtaacac agggattttc ctcctcccgt gggcatcacg
    12061 accaatacat tgcggccttc cataatggaa gccaccactt gatcctggcc ctccctcaag
    12121 caggaaaagc cgaagtactt cttcaatgcc tccatcggcg acctgatcac cttgaactcg
    12181 ctcattttct ccgtttagtg tgccacaggc cattccatgg cgcaacgcct ttcccctttt
    12241 tctccacccc tcagttaacg gttctttctc tgaaaattta ttctcccaaa aagagaaaaa
    12301 tgaatttttt tctcaaatca tcttacaaac atcttgactt tcccgaagaa aaatctatat
    12361 ttccttcacc gcttacacgg tacacgacgc aagtcaaagc gcccgtagct caattggata
    12421 gagcgtctga ctacggatca gaaggtttgg ggttcgaatc cctacgggcg tgccacttgc
    12481 atcactgcca gcaaataaaa tgattcctgc ctgaacatca cggcgggatt ttttgtctct
    12541 actggtaaat acatatgggg gcgccatggt ttcgactgga agtctgcggt gctggctgca
    12601 tgtgaaggtt aatcggctgg ccttctaaaa agccgattaa aaaaataaat gcagagtcta
    12661 acgaccttgc tctggctgcc taattagttc agccgcgtca gaacctcaac gcctgctaga
    12721 ggggacgacg aagacacagc aggctggtgc aatgcggggt tgccgcgcaa cgcacgagac
    12781 ttccacaggc aactagcgag cttgcctagt tgacgcatag cgaagcagcc gcgaaagcag
    12841 caatatgcgt ctgaacatgg agatgccgtc atcaatggcc ttcaggacac gggttcgact
    12901 cccgtcgcct ccaccatttc aaaacaaccg caagtctttg aaaaacaagg cttgcggttg
    12961 ttgtttttat agaaccgcag tacatgataa cggcttttag aaacaatgta ctgtattttt
    13021 aaagccgcat gacgatactc catccaggtt ttccgataaa catcagagca taagggaatc
    13081 tgcaattttt gaggcatcac gacagaccag gcccttcctg ttcccaacgt tgaccaggaa
    13141 ctgctttacg tcactcatgg ggttactgtc attgattccc tgtttttgat ccgctcctgc
    13201 ctgggaggaa catcttttcc atttaaaact gttgcaatgc atcgacaaat ccggaaacgc
    13261 cttgtccggg aaaaaacact gcatcacata tcccccagtc tgccgcaatc atgcagtcag
    13321 tccaacaatc ttatttgctg atatccttat tattttctga tggtaaaata cttgaaaaac
    13381 gatatggagg caaaatccat cagtatgaac tgcgcataat taccgggggc aaaaatacgg
    13441 cggcccccga ggctattgac agcctcaatc gtcagtagaa ctgcttcacg aaactcaacg
    13501 gcctcatcgt ctcctgcgga attattgaga acagaggtat ttcccagcct tgcccatacc
    13561 tggacggctt gccagtcctc ttccatgtct gcaatgacgg ctacagccgc gctcaaagcc
    13621 ggggcctgcc cggacggtat atcattctgt ttatagcggt ctatgcgggt atatccatcc
    13681 gaatcctggt agatggctgt tagtgtgaat tccctccagt taccgggctt ggaaaactgt
    13741 atttgtatct ctctcatggt atcattatgc taattggctg atttaataat ggcggcgcga
    13801 gcatcgtgat ggctggcaaa cacccacacc tgccgggctt gtcccgtctt gttggaggaa
    13861 taacatcagg gatatttgtc tgcctccggc tgatctttct tcagggagct gactgcacct
    13921 aacacccact tccttgaaaa atctgctcca gctcgcggaa aatgtcatcc tgtgtaaaac
    13981 gtccagattg aaagtccccg ctcccgcccg gcattctggc caacattgat gaggatacgg
    14041 cccgtaacaa atatgatatg ccccctcgtt tgaagccgta tggaagatgc tttgcctaaa
    14101 tcgcagatga gacagcctgc ggagcattcc tatattgtca cacccactta actatttgaa
    14161 tacaataaat gatgatatcc tttctctata aaaccatcct atcacaggaa aggaacgaat
    14221 tattggaaaa attgctttga aacccggatt cggcccctgt ccgtaaaaaa ctatacgatg
    14281 aaatgagctt cccgaatatc cagaagcgtt gataaaaata cttcaagcca acagtatgta
    14341 ctttatatgg aaaactttta tcatcaaaca acacgccgga atttcctgaa tcaattaggc
    14401 ctggcggcag caggaatgac aacgggcaat acagcccttg ccactacaca atatgattcc
    14461 ggcattccca gaaattccgt accagataga acttccgata attccgccaa aagagtgatc
    14521 atgatttccc tggacggtat ttgtgtggaa ggttttcaaa aggcgcatgc gccctcttta
    14581 aaaggaatga tgtcacaggg agctttttct ctggatacgc gggttgtgat accctccgtc
    14641 acgttaccta actggaccag tcacttaacc ggcagcggcc cggaacagca cggagtcttc
    14701 aataataact ggacgccggc aaattcctcc taccccgccg tacggagaga cgaagacgga
    14761 tattatccct ccgtattcca ggccctgaaa aaatgccagc cttcctgcaa aacagctttt
    14821 tactataatt ggaaacctct tgcctatccg ttcaacgaaa aatacctgga tgaaatcaag
    14881 tacctggcaa acgacgccta tgcttccaat taccatcagg ctttccaatt cgtcaagaaa
    14941 caccgctaca acccgactgt ggtttttctc tattccgttc atacagatca tgcggggcac
    15001 aaatacggct ggatgtccag agaatacctt caatctattg aagaggcgga tcagcaaatt
    15061 ggaatctttt ttcagaaatt gaggcaggaa gacttattca acgatacgca ttttctattt
    15121 ctgacggatc atgggggaat cggaaagggc cacgggggga cgagcgcagc ggaaatgata
    15181 gttccatggg gaatttcagg tccgggcatt aagagaaatt tcctgatttc cgaaccaaac
    15241 aataccacca ataccgctcc catgcttctt catttattcg gaggaaaacc actaccggaa
    15301 tggacgggga aagtcattga aagcgtgttt ccatcataac tccccgtagc ttgcttttcc
    15361 tgatgcaatt taccgcctct tcacggcagg aaacaaaaca tggctgccgg atggcattcc
    15421 ctgccggagt tgaaccgagc ctttgccgtt ggagagacgg ccttcctgac cgctagaaga
    15481 agggaacatg acagccggaa taaaacagcc cgcttttcac aagccgctga aatctgcata
    15541 gcactttccc ggaaagagtc aatgattttt accgtaccct ttcccctttt taatcaaata
    15601 tggaattcca acagggagcg tcagccgcat caaaacgcaa cagaacctgt tccagagtgt
    15661 tttttccaaa gacatggagg caagcttatt ccgaccagag catcagcttt ctgatgtttc
    15721 ccaaccatcc ttttttcaac cgttgcccac ccatacgcat aagataaggg gaaggattgc
    15781 acctgtttct tccatgaagg acaatgaaac gtacggccac cacctccgac cgtcttcatg
    15841 acgaattgtt cactcaggct ttctggcctt gttcatcttc atggcaaatc atcccacgta
    15901 gccgacatga ttggaccatt cagcatttaa tgattcgagg cgcgggcaca ccctccttct
    15961 gggttgccgt accgcatcaa agacatctta tgacgagccc tgccgcatga ggtattgttc
    16021 atttttcctc ttttatttcc ggcaaaacca caggttctcc caaaaaacgg ggttcacggc
    16081 tccaaaccca caagtccagc atcatccata cgcgggccgc acgctccctg aaccagcttt
    16141 tcatacggga acggcggttg ggaacatctg gcgcgctgac ggcccaggcc gccatc
//
LOCUS       DAOMSU010000019        16181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_127_length_16181_cov_2.764480, whole genome shotgun sequence.
ACCESSION   DAOMSU010000019 DAOMSU010000000
VERSION     DAOMSU010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 16181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16181
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127_length_16181_cov_2.764480"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="PK077_01710"
     CDS             complement(<1..1128)
                     /locus_tag="PK077_01710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23048.1"
                     /translation="MDQDRILYRGEAFTLTGNSLRQDAAHWAEVQPDGQVKTMKNGRY
                     SEWRIFPEAGNAPHYRGNFEVLNQAYGLALHEAGALLNEEGTFRTGANWPTVWTRDIS
                     YSTHLGLGLWNVRACMNSLNARVRNGEVEQDTGTGGSWPISSDRVVWGMAAWEVYCLT
                     GDADWLSLSCRVMEKTCMRDEQVLAATGGLMKGESSVLDWREQSYPAWMTSADIGDSC
                     SLSTMLLHAEARKILARMFRELGLEEKAREWEEKSVSLAAVIERFFRIPEHVLYGQYL
                     YGRGYPVLSEKVDSLGNLLCVLLGQAGGSHAAGMVASLPHGVYGIPCIHPQMPDSVPA
                     YHNRAMWPFLEGYYAQAAAAVENESALALAVACMVRAASARA"
     gene            complement(1273..2262)
                     /locus_tag="PK077_01715"
     CDS             complement(1273..2262)
                     /locus_tag="PK077_01715"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HRN23049.1"
                     /translation="MKTPITVTVTGAAGQIAYSLLFRIASGSMLGPDQPINLRLLEIP
                     PAMNALEGVVMELRDAAFPLVNEIVPTSDPDEAFAGANWCLLVGSVPRKAGMERKDLL
                     DINGKVFIGQGQAIARSAAKDVRVLVVGNPCNTNALIAMHNASGVPSDRFFAMTRLDE
                     NRAKSQLAEKAGVHVTEVTNMAIWGNHSSTQYPDFTNARIGGKPVTEVIKDTEWLKGD
                     FITTVQQRGAAIIKARGASSAASAASAAVDTVRSLATQTPEGDWYSVAVCSDGSYGIE
                     KGLICSFPVRTTKDGGWEIVQGLPVDAFSREKIDATVNELKEERDAVSSLLKH"
     gene            2447..3769
                     /locus_tag="PK077_01720"
     CDS             2447..3769
                     /locus_tag="PK077_01720"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRN23050.1"
                     /translation="MNLTIIGSGYVGLTTGTCFAEMGHHVICVDNNTEKIRTLQSGAI
                     PIYEPKLEELVKKNVAVGRLEFSPSIAASIAESEVIFIAVPTPPNTDGSVDLTYIEKV
                     AREIAQALQPEMGYKVIVDKSTVPVKTGEKVSQTIKHYAGPNVQFDIVSNPEFLREGC
                     AVDDLLHPDRIVIGANSEQAMNVIKRVYQPIHAPILETDVNSAELIKHAANSFLALKI
                     SYINAVAKVCEKTGADVELVAEGIGMDKRISRHFLNAGLGYGGSCFPKDVKAFINISR
                     TLGIPFTLLEEVEHINDTQHIHFLDRIRDRLWVLKDKKIAVWGLAFKQNTDDVRESIA
                     LKLCEKLCGEGAIVTATDPKAMHTAAPILNPMGVKLVEDMYECARDAEVLVIATEWSE
                     YANADLQKLAGVMRNRIIFDGRNILSPANLRAVGFEYHSVGRPSVEEA"
     gene            complement(3879..4859)
                     /locus_tag="PK077_01725"
     CDS             complement(3879..4859)
                     /locus_tag="PK077_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1434 family mucin 2-degrading aspartic
                     protease"
                     /protein_id="HRN23051.1"
                     /translation="MLLRFLSSLFFASGIVLTAQANTQPYPDSDSSDGYDTGFYAEAY
                     SGGYDSAPAPAGESASKPVFPTQSYFELLGPMDSRNGRGSVDIQNWMTDLNLCRMSSG
                     SWNFSSTAALRLSWFNGKGADEMDVDRLYTIWLNVNASYAITGKTRLVFGVTPQISTD
                     FDTWTSHNIFLGGHVLLAGPLNDRFSYGIGIGCYPQLGDYPFLPLIQFKWEASRNWTL
                     QLEGARLSYINKVSDGLKWGPFVSVVSGTWTIHHDRRVRQFAWISGVAGIGADVGLGH
                     WGSVRPRLVADVGFSFGNSGTIKTSNGSHDLEKYHYDPGFYLRAGLLFEF"
     gene            complement(4918..5202)
                     /locus_tag="PK077_01730"
     CDS             complement(4918..5202)
                     /locus_tag="PK077_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23052.1"
                     /translation="MMRPVRQKTFSVSWLAGLRWSFGGYVTGTLLAAGAGALDFPLLA
                     QVMLLAAAVCFAASLVCAGACFRGSKIARWYLLLVLVMIMVAVLFGNGGG"
     gene            5576..6007
                     /locus_tag="PK077_01735"
     CDS             5576..6007
                     /locus_tag="PK077_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein NlpE"
                     /protein_id="HRN23053.1"
                     /translation="MKINFLWTACLIAITGCDSPKNHHSRIESGETKTEKQLENLNFY
                     GTYEGILPAADCEGIRTALTLNNDNTYVLRSEYIGEKDAIFESKGSYHFINGRLMELA
                     QSSSNEKSYYKILDGSKVMLSDKEGTVNQGVLAEYYILKKK"
     gene            complement(6395..6471)
                     /locus_tag="PK077_01740"
     tRNA            complement(6395..6471)
                     /locus_tag="PK077_01740"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6435..6437),aa:Pro,seq:tgg)
     gene            complement(6507..6582)
                     /locus_tag="PK077_01745"
     tRNA            complement(6507..6582)
                     /locus_tag="PK077_01745"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6547..6549),aa:Phe,seq:gaa)
     gene            complement(6715..7581)
                     /locus_tag="PK077_01750"
     CDS             complement(6715..7581)
                     /locus_tag="PK077_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23054.1"
                     /translation="MLKRPPWWAWFSLMGLEIPSAAIAWAFAVSQSHLVVVSSPWLYQ
                     FLFVAVWCVNMLDRVLKIFRGGSAVYTDECLLFAKKHCFVISLLIMVAAVTGLWIMFF
                     QLGVVIFQFAFLPGVCSLLFLYFSSNPHQKGSLSSGFVMGAFFAAVSFATGAGIPAYF
                     YGTVGGGWGGQLSEPTWYLVALVILCMFSRKRWSEEEDEDGQDSADRDMVSLVWMGVI
                     VIYVVFCLAAAAREYTGDAWIYYGLAMAGAFMFVLDRLKMNMPPLLLHTLSWAALIFP
                     LLLSGILAQASS"
     gene            7742..8017
                     /locus_tag="PK077_01755"
     CDS             7742..8017
                     /locus_tag="PK077_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HRN23055.1"
                     /translation="MKKDIHPQYNPVVFVDITTGRRFITRSTVRSPKTEVIDGVEHFV
                     VSCGITSDSHPFFTGKNQFVDTEGRIDKFQKRFGTVRRSGGKPKLNK"
     gene            8124..9128
                     /gene="tilS"
                     /locus_tag="PK077_01760"
     CDS             8124..9128
                     /gene="tilS"
                     /locus_tag="PK077_01760"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRN23056.1"
                     /translation="MLTPDHFDSQSAELLRSGRPVLAGISGGRDSMALLSLLSGMPGC
                     NLVACHVNHQLRPEAEEEEMFVLRYAASLGIPCVRERVNVRSMAHTRGIAIEEAAREA
                     RQASFLKWAAGYPGALVALAHHRNDQQETALLHLCRGASGIHGMFPVSTWANGLTVVR
                     PMLDFSREDITSYLEHRHIPWREDSSNQSAEYTRNALRHEIIPKLNALFQRDTSLPFS
                     RACRLENQIRTALSQALDSMNLTDPQGRLFLPKINLLPAELRQCAVHDYLRRQHIPDL
                     TENAVLRVMDILNTDGPSRTSLPGGKLAVRKEKRLLVTNTPRPVNKAEQPPADTTGGI
                     "
     gene            complement(9082..10152)
                     /locus_tag="PK077_01765"
     CDS             complement(9082..10152)
                     /locus_tag="PK077_01765"
                     /inference="COORDINATES: protein motif:HMM:NF013665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HRN23057.1"
                     /translation="MSDWQGFSPLNDFTGPLLDNLKRHPKRIVFPEGEDVRVLRVSQR
                     FVEEQAGAPILLGRREVITRMAEMNGISLKFVRIIEPEKSSDLQMFCDRYERAEQMFG
                     NGLPMNTREIVSEPVHFAAMMVLYGQADAIVAGNMKRVNKYRQDPVPTKPLFAISIVL
                     VPEFSKKFGGRGVYFLADTGVNPEPTVENMAYFAVETAKMARHMLGKSVRVAMLSAST
                     SGSVPELAADRTRAAAALAKSMVQKDCLNNEISIEGEIQIDAALSSDSYSVRVHRNSM
                     LQPSEVWVFPTLDAADISKKLLCMMPSVYNYGLILGGLLFPIAQLPRLTDEDRMFGTA
                     LVVGNEAIKFHLLYPQGVAPLY"
     gene            10273..11106
                     /locus_tag="PK077_01770"
     CDS             10273..11106
                     /locus_tag="PK077_01770"
                     /inference="COORDINATES: protein motif:HMM:NF005596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HRN23058.1"
                     /translation="MLAWAFPSMYCDYHTHTPLCLHASGTPQEYVQAAVRAGLREYGI
                     SDHAPMPGEPFDDWRMKQADMPAYLDWLTEARECAALHGLTVRSALECDWFPGIEPWI
                     EHLQGLHAWDYLIGSVHYLGEKEEFDNPHRMDFWHRTDVEDAWEQYWERFRNMAASGM
                     FHIMGHADLIKKFGFRPSGDLRRYYEPSLEAMRETGACLELNTAGWHNKCGEQYPDGQ
                     FLKMAAEAGIPLTISSDAHRPEDVGRDFKRAGELARQAGFRHLASFQGGRMKLFPLPG
                     I"
     gene            complement(11255..12088)
                     /locus_tag="PK077_01775"
     CDS             complement(11255..12088)
                     /locus_tag="PK077_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23059.1"
                     /translation="MKTGTRLLILCLLAPAALAENMSLRSGRVLKDAVVVSSGDDFVN
                     IQYTDGVAKVSYKDLTDAQQRDYRMTPDEVKARLDQRRMEEAARRKKAEEARKAADEE
                     ARKIRESLSEAERHPRYLEGADISRMFLLMGELTKVEAEVMALQWNALEADRVGLPED
                     ARVFRARAAAYQEQISAIRKKRETAEQYWQDLEKKYLALKDGTQKKIADLNKQVSQLQ
                     GEVREVASQPRTERIIVPPIIRTWNYPPPPVFIRPQPPPPRPVPPRPMPLPVKPSLYR
                     S"
     gene            12350..13201
                     /locus_tag="PK077_01780"
     CDS             12350..13201
                     /locus_tag="PK077_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23060.1"
                     /translation="MLEGGAKTWTDRIFTLSPGENEWKEAGTLPAPYAYGASAGIREG
                     LLCIGGCGKEGHRKDVYLLNLAEGTVTTHPFPSLPIALAYTAAAVLDGKVYLTGGCER
                     PGEQDCTNRVFMLDTRRTEQGWLEQAPLPGRGRFLHQMAATDGALYVLGGIGLREQDG
                     QQVRELLKEAWSYTPEHGWTRLPDMPYAIAAAPTPAPVSGNGVIYLLGGDDGSGKKYT
                     PQTNPGFNNQSLCLDTADMTWHDAGPIAAPRAVLPCCAWQDRFAAVNGELKPGRRSPE
                     VWSISLP"
     gene            13240..15786
                     /locus_tag="PK077_01785"
     CDS             13240..15786
                     /locus_tag="PK077_01785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN23061.1"
                     /translation="MTRTLTRISKACGSALAALLLIAAPAADASFSVDVRTSGTVPDL
                     PDPAGRAGMVAGTVTEDDGSQSVIAAGGANFPQAAPGASTPEERGPKTYYQDIFKLRN
                     GQWSKAGTLPVPLGYAAFASVGKGLAVAGGHNAQGILKDALLIKTDGSVEKLPPLPVP
                     VTEASCAAQGNKLFVIGGRDREQPETALNTIYMLDTTPDTDKMKWVSLPPFPGEGRIL
                     STAAVCDSTLFIIGGCSLSRDNSGETSRTYLSDMIGYDMTSKDPSKWGSSGRQQLAGP
                     GMPVAAAAGPAPVRENSILLIGGDKRGNSPDPSRPVAQSRDILVYDVIGNTWTRQGEW
                     PVGIATVPAVVRGSEIMTISGETAPGVRTPVNASASAGYHFEMSTVDYAVLILTIIVL
                     AIIIVSAVRNGVKNVASVTDPNTKPGLWAWVAVIVLWFVVMLNYFDRQLLSALHEPIV
                     RDIPQTEAQFGMVTSVFLLIYALLSPVGGFLADRYSRRLMILCSLVVWSVVTWWTGHA
                     EDYTSLLIARGAMGISEAFYIPAALALITDYHRGSTRSIATGLHMSGIYVGMAVAGFG
                     ATMASWTGWRMTFALFGLIGVAYAVILILFLKDPSKAPADTAQAKKPSVPEKKTVLLN
                     VDNDEQAIKEPSSKLSTGAVLSSLLSGRPMWMLLAVVAFAGAGNWFLLTWYPTLLQDK
                     YQLSSAEAGPAATLWSSVAKYVAVLGGAILADMWYRRNARARALVPGITFTISGPLVV
                     LALLPGIFGWDITVPLVLMLGLVATQGLAQGSLDATLMPVLRSHIDERYSATGYGLLN
                     LTSAGVGALISFFGGWFKDQGVPLTTTLAAAGCLMLFCGLLLLMLPRPKH"
     gene            15801..>16181
                     /locus_tag="PK077_01790"
     CDS             15801..>16181
                     /locus_tag="PK077_01790"
                     /inference="COORDINATES: protein motif:HMM:NF015916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008092 - cytoskeletal protein binding
                     [Evidence IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0043130 - ubiquitin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SHD1 domain-containing protein"
                     /protein_id="HRN23062.1"
                     /translation="MPPVLPLMKKPARLCLAVGTLFALFLTPLAQGRTWTNLQGKKLE
                     AEFIRLDGQKAVLKRSGGQTVSIPLSQLSREDRDFIAEQEKGGALPSNTADNYHLPWP
                     RSVKCPDNFKVETIKEEPGEYIYET"
BASE COUNT         3813 a         4972 c         4147 g         3249 t
ORIGIN      
        1 tgccctggcg ctggcggcac ggaccatgca ggcgaccgcc agcgccaggg cagattcatt
       61 ctccaccgcc gccgcggcct gcgcataata gccctccagg aaaggccaca tggcgcggtt
      121 atggtaggcg ggcacgctat caggcatctg gggatggatg cagggaatgc cataaacgcc
      181 atgaggaagg gaggcaacca tcccggcggc atgggaaccg ccagcctgcc ccagaaggac
      241 gcaaagcaga ttgcccaggg aatccacctt ctccgacagt acgggatacc cgcgtccgta
      301 caggtactgc ccgtacagga catgttccgg aatcctgaaa aaacgctcaa taaccgcggc
      361 cagggaaacg ctcttctcct cccactcccg ggccttctcc tccagcccca gctcccggaa
      421 catccgcgcc agaatcttgc gggcctccgc atgcaggagc atggtggaca ggctgcaaga
      481 atccccaata tcggcagaag tcatccacgc agggtagctc tgctccctcc agtccagcac
      541 ggacgattcc cccttcatca agccgcccgt ggccgcaagc acctgttcat ccctcatgca
      601 tgtcttttcc atcactcggc aggaaaggga cagccagtcg gcatcccccg tcaggcagta
      661 aacttcccac gcagccatcc cccacaccac acggtcggaa gaaatgggcc aggaaccgcc
      721 cgtgccggta tcctgctcca cctccccgtt ccggacccgt gcattcagac tgttcataca
      781 ggcacggaca ttccacaacc ccaatcccag atgggtggaa taggaaatat cccgggtcca
      841 cacagtaggc cagttggctc ccgtccggaa cgtcccttct tcattaagca gggctccagc
      901 ctcatgcaac gccaggccgt aagcctgatt aagcacttca aaatttcccc ggtaatgcgg
      961 ggcgttcccg gcttccggaa agatgcgcca ctcggaatag cggccgtttt tcatggtttt
     1021 cacctgaccg tccggctgta cctccgccca atgcgcggca tcctggcgca aggaatttcc
     1081 ggtcagcgta aaagcctctc ccctgtataa tatgcggtcc tgatccataa actagcggat
     1141 gctgcatcca tcgttttttt ttaacacgga agaaaacttc tgtcccgcag aaaatgaggt
     1201 ttatggaatc ttcaggacat aaaaaaccgg gcttcccgaa aacgggaaac ccggcgcaag
     1261 agcaaatcaa cattaatgct tcaacaaaga ggaaacagcg tcacgttctt ccttcagttc
     1321 attaacggta gcgtcaattt tttcacggga gaacgcatca accggcagcc cctgcacgat
     1381 ttcccagccg ccatccttgg tggtgcggac cgggaaggag cagataagac ctttttcaat
     1441 gccgtaggaa ccgtcggagc aaacggccac ggaataccag tcgccttccg gagtctgggt
     1501 agccaggctg cggacggtat ccacggctgc ggaagcggcg gaagcggcgg aagaagcgcc
     1561 gcgtgccttg ataatggcgg caccgcgctg ctgcacggtg gtaatgaaat cgcccttaag
     1621 ccattccgta tccttgatga cttccgtcac aggctttccg ccaatcctgg cgttggtgaa
     1681 atcagggtac tgggtggagg aatgattgcc ccagatggcc atgttggtca cttccgtcac
     1741 atggacgccg gccttttccg caagctggct cttggcacgg ttttcatcca ggcgagtcat
     1801 ggcaaaaaag cggtcggagg gaacgccgct cgcattgtgc atggcaataa gggcattcgt
     1861 attgcagggg ttgccgacca ccagcacacg cacatccttg gcggcgctgc gggcaatggc
     1921 ctgcccctgg ccgatgaaca ccttgccgtt gatatccagc aggtccttgc gttccatacc
     1981 ggccttgcgg ggaacggagc ctaccagcag gcaccagttg gcgccggcga acgcttcatc
     2041 aggatcgctg gtcgggacaa tttcattaac cagcgggaac gcggcgtcac gcaactccat
     2101 caccacccct tccagagcat tcatggccgg gggaatttcc agcaggcgca ggttgatcgg
     2161 ctgatccggc cccagcatgc tgccggaggc aatacggaac agaagggaat aggcaatttg
     2221 gccggcggct cctgtaacgg taacggtgat aggtgttttc ataacgttca aattaaaata
     2281 aacagatatt ttcctgtctg gtcacggtta taccacaagg aaaggaggcg ctcaatcagg
     2341 aactttttat tttgacagaa aaaattccct atcccataat gctcaggggt tgactgctct
     2401 tacttttcca tacagttaag tttttaccaa tatttacgct aacactatga atttaacaat
     2461 tatcggcagt ggttacgtgg ggctgaccac aggcacctgt tttgccgaaa tgggccatca
     2521 cgtcatttgc gtggataata atacggaaaa aatacgtact cttcaatccg gggccatccc
     2581 catttacgag cccaagctgg aagagctcgt gaaaaaaaac gtagccgtgg ggcggctgga
     2641 attttcccct tccatcgccg cttccatcgc ggaaagcgaa gtcatcttca tcgccgtgcc
     2701 cacgccgccc aatacggatg gttccgtgga cctgacctac attgaaaaag tggccaggga
     2761 aatcgcccag gcgctccaac cagaaatggg atacaaggtc attgtggaca aatccaccgt
     2821 acccgtcaaa accggggaaa aagtcagcca gaccatcaag cactatgcgg ggccgaatgt
     2881 tcagttcgac attgtctcca atccagaatt cctgagggaa ggatgcgccg tggacgacct
     2941 gctgcacccc gaccgcattg tcattggagc caactcggaa caggccatga acgtcatcaa
     3001 gcgcgtttac cagcccattc atgcccctat tctggaaacg gacgtcaact ccgcggaact
     3061 catcaagcac gcagccaact ccttcttggc tctgaaaatt tcctacatta acgctgtcgc
     3121 caaggtctgt gaaaaaacgg gcgcagacgt ggaactggtg gcggaaggca tcggcatgga
     3181 caaacgcatc tcacgccact ttctcaacgc ggggctgggc tacggcgggt cctgcttccc
     3241 gaaagacgtc aaagccttca tcaacatcag ccgcacgctg ggcattccct tcaccctgtt
     3301 ggaggaagtg gaacacatca atgataccca gcatatccac ttcctggaca gaattcggga
     3361 ccgcctgtgg gtcctcaagg acaaaaagat agccgtttgg ggccttgcct tcaaacagaa
     3421 cacggacgac gtccgggaat ccatcgccct gaaactatgt gaaaaactct gcggggaagg
     3481 agccattgtc accgctacgg accccaaagc catgcacacg gcggccccca ttctgaaccc
     3541 tatgggggtc aaactggtgg aagacatgta cgaatgcgcc cgggatgcgg aagttctggt
     3601 catcgccacg gaatggagcg aatatgcgaa tgcggatctg cagaaactcg ccggagtcat
     3661 gcgcaaccgc atcatctttg acggaagaaa catcctctcc ccggcaaacc tccgggccgt
     3721 aggatttgaa taccactccg tgggcagacc ctccgttgaa gaagcctgat tgcctccggg
     3781 aaccgtcccg gcttcaaacg tccccatatg taaaaggcct ccggcacatt caacgcaccg
     3841 gaggcctttg tcatgtattc gctaatcctg ttggccggtc aaaactcaaa tagcagcccc
     3901 gcacgcaaat aaaaaccggg gtcataatgg tatttttcca aatcatggct tccgttgctt
     3961 gtcttgatag tgccggaatt cccgaaggaa aaaccgacat ccgccaccag cctggggcgc
     4021 acgctgcccc agtgccccaa ccccacatcg gcgcctatgc cggcgacgcc ggaaatccat
     4081 gcaaactggc ggacgcggcg atcatggtga atcgtccatg tgccggacac cacggaaacg
     4141 aagggtcccc acttcaaccc atcgctcacc ttgttgatat aggaaaggcg ggccccttcc
     4201 agctgaagcg tccaattgcg ggaagcttcc catttaaact ggataagggg caggaaagga
     4261 taatccccaa gctgcggata acagccgatg ccgattccat agctgaaccg gtcgttcaag
     4321 gggcctgcca gaagaacatg gcctcccaag aaaatattat ggctcgtcca tgtatcgaaa
     4381 tccgtggaaa tctggggcgt cacaccgaaa accaggcgcg tcttccccgt gatggcatag
     4441 gaggcgttca cattcagcca aatcgtgtac agccggtcca catccatctc atccgctcct
     4501 tttccattaa accagctcag acgcaaggcc gccgtagaag aaaaattcca ggaaccgctg
     4561 gacatcctgc acaaattcaa atccgtcatc caattctgga tatccacgct tccgcgcccg
     4621 tttctggaat ccatggggcc aagcaattca aaataggact gcgtggggaa aacaggcttg
     4681 gaggcggatt cccccgccgg agccggagcg ctgtcgtagc cgccggaata agcctctgca
     4741 taaaaaccgg tatcgtatcc gtcagaagaa tcggaatccg gataaggctg ggtattcgcc
     4801 tgagcggtca aaacaatgcc ggacgcaaaa aagagactcg ataagaatcg aaggagcatg
     4861 accggaaagt atacatacgc gcgcacgcgg tcaatcaaaa gatatacaaa tgactaatta
     4921 accgcctcca ttcccaaaca gaaccgcaac cataatcatc acgaggacaa gaagcaagta
     4981 ccaccgggca atcttgctgc cccggaaaca cgctcccgca caaacaagag aggcggcaaa
     5041 acaaaccgcc gcggccaaaa gcatcacctg cgccagcaag ggaaaatcca gcgcccccgc
     5101 acccgccgcc agaagtgttc ccgttacata cccgccaaac gaccacctca accccgccag
     5161 ccaggaaacg gaaaatgtct tttgtcggac gggcctcatc aagccactct gcaagccttc
     5221 ttcctaatct caagcaaaaa atccagcctt ctcggcaata tctgcaaaga gggggcttcc
     5281 ccagacactc ggctcccctc aaaaaaaaca acatcttcgc aaaacggact acaaaacact
     5341 caatcccggt tagaaaactc ttcttccggc aaagttgaat aaaaacggga taaattactt
     5401 aatccggcat tcatcaatac tcgcgggctc catgaaaaga aaatattttg ccccttccat
     5461 agactttcat tatattacgg caaaaccgga aaaacaggaa acgaacgtac tcccttccct
     5521 gccccattgg atcaagcatc gcacgtcaca aaccaccagc tgaaaaaaac ttgtcatgaa
     5581 aataaatttt ttatggaccg catgtttaat tgccataacc ggctgtgatt cccccaaaaa
     5641 tcatcattct cgaattgaga gcggcgaaac aaaaacagaa aaacagttgg agaatttaaa
     5701 tttttacggc acttatgaag gaattcttcc tgctgccgat tgtgaaggaa tcaggacggc
     5761 gttaacatta aacaacgata acacttatgt gttgagaagc gaatacatag gagaaaaaga
     5821 cgctattttc gaatcaaaag gctcttatca tttcatcaat ggccgcctga tggaactggc
     5881 ccaatcctct tccaatgaaa aatcttatta taaaatactc gatggaagca aagtaatgct
     5941 gtcagacaaa gaaggcacag tcaatcaggg cgtcctggcc gaatattata tactaaaaaa
     6001 gaaataaaac tatttctatc atctatcatt ttctccattc cgccgcacct cccaccccgc
     6061 ggcggttttc tttacaccgt ttttcagccc ggcacattaa caaatcttat tcatccgccc
     6121 cgccgtcaga aaaaatccgg gccgccggga ccaggctttc gtcagcacac gggtgttaca
     6181 agaactttgc atcgcctttc tgtccgcgtt gtttcggaca caaaagattt ccggcttttt
     6241 taactggctg caggttcggc cgccccgccg caacaggggt gtttttcatc tttccaggca
     6301 taccccgctt ccactccatc tattttacgg acaaccacca gggaacagct caaaaaacaa
     6361 aacccgtaaa tcatttgatc cacaggcttt aaaatggtcg ggaagacagg attcgaacct
     6421 gcgacatcct gctcccaaag caggcgctcc accaggctga gctacttccc gtataaaaag
     6481 gagggattag aaattgggag aaaaaatggt ggctcgggac gggatcgaac cgccgacacg
     6541 gggattttca atcccctgct ctaccgactg agctaccgag ccacttgcct gcacgcagaa
     6601 cgtgcttgcg gggcagaatc tatcatcctc ttcccaatat tgcaaccaca aaatattatt
     6661 ttttcaactt ccctgtattt tgccgcgctc catagcagga atccgcccag ccaatcagga
     6721 ggaagcctgc gccagaatac cgctcaacag caatgggaaa atcagtgccg cccaggatag
     6781 cgtgtgcagg aggaggggag gcatgttcat cttcagcctg tccagcacaa acatgaatgc
     6841 ccccgccatt gccagcccat aataaatcca cgcatccccg gtatattccc tggcggcggc
     6901 ggccagacag aatacaacgt aaatgactat cacccccatc cataccaggg ataccatgtc
     6961 gcggtcggcg gaatcctgcc cgtcctcatc ctcttcttca ctccaccgtt tgcggctgaa
     7021 catgcagagg atgaccagcg ctaccagata ccaggtgggt tcggaaagct ggcctcccca
     7081 gccgccgcct accgtcccgt aaaagtaggc cggtattccg gcacccgtag cgaaggaaac
     7141 ggcggcaaaa aacgccccca tgacgaatcc ggaagacagg ctccctttct gatgagggtt
     7201 ggaagagaaa tagagaaaga gcagactgca gacgccaggc agaaaggcaa attgaaaaat
     7261 aaccacaccc aattggaaaa acatgatcca aagccccgtc acggccgcca ccataatgag
     7321 cagggagata acgaaacaat gctttttggc aaaaagcagg cattcatccg tgtagacagc
     7381 ggaaccgccc cggaatattt tcagcacccg gtccagcata ttgacgcacc agacagccac
     7441 aaacaggaat tgatacagcc agggggagga aactaccacc agatgggact ggctgacggc
     7501 aaaagcccag gcaatggcgg cagaagggat ttccaatccc atcagggaaa accacgccca
     7561 ccaggggggc ctcttcaaca cctgctccat ttttccgtca tatcatctcc acaaccgttc
     7621 cttccgcaag ctcccgaacc gtcatccaca aacttcctta tttttacgcg gggatgcttg
     7681 acatgcaatg aaacatattt atacttgcgt tccccttgcg tcggaaacgc aatacatcac
     7741 catgaagaaa gatattcacc cccagtataa tccggtcgtt tttgttgaca tcaccaccgg
     7801 acgccgcttc atcacccgtt ccactgttcg ttctcccaag acggaagtta ttgacggggt
     7861 tgaacatttc gtggtttcct gcggtatcac ctctgattcc cacccgttct tcaccggcaa
     7921 gaaccagttt gtggacacgg aaggccgcat cgacaaattc cagaagcgct tcggcaccgt
     7981 ccgccgcagc ggaggcaagc ccaagctcaa caagtaagaa ctgccttcag caattttcca
     8041 accccgtttc cttcccggaa aacggggttt ttatttgcag gcttcctttg atctcttttt
     8101 cctgattcac tcgcagcagc accatgttga caccggacca ttttgacagc cagtccgcgg
     8161 aactcctccg ctccggccgc cccgtccttg ccggaatcag cggggggagg gactccatgg
     8221 ctttgctttc cctgctgtcc ggcatgccgg gatgcaatct ggttgcatgc catgtaaatc
     8281 accagctgcg tccggaagcg gaggaagagg aaatgtttgt actccgttac gccgcctccc
     8341 tgggtattcc ctgtgtccgg gaacgcgtca atgtccgcag tatggcccat acccgcggaa
     8401 tagccattga agaagcggcc agggaagcca ggcaggcctc gtttctgaaa tgggccgccg
     8461 gttatccggg ggctctcgtg gctctggccc accaccggaa cgaccagcag gaaacggccc
     8521 ttcttcacct ctgccgcgga gcctccggca ttcacggcat gttccccgtc tccacctggg
     8581 ccaacggatt gaccgtcgta cgccccatgc tggatttttc ccgggaggat atcacctcct
     8641 atctggagca tcgccatatt ccatggaggg aagattccag caaccagtct gccgaatata
     8701 ccaggaacgc cctgcgccat gagattattc ccaaattgaa tgcgctgttc caaagggaca
     8761 ccagccttcc cttttcacgt gcctgccgcc tggaaaatca aatccgcacg gcgctttccc
     8821 aggcactgga ctccatgaac ctgacagatc cccaggggcg cctgttcctg ccgaaaatca
     8881 acctgcttcc tgcggagctg agacaatgcg ccgtgcatga ttacctgcgc cggcaacata
     8941 ttccggacct gacggaaaac gccgtactcc gggtaatgga catcctgaat acggacggcc
     9001 cttcccgaac ttccctgccc ggcggaaaac tggccgtacg caaggaaaag cgcctgctgg
     9061 tcacgaacac gccgcgcccg gtcaataaag cggagcaacc ccctgcggat acaacaggtg
     9121 gaatttaatg gcttcattcc ccaccaccag cgcagtaccg aacatcctgt cttcatctgt
     9181 cagccgcggc agctgggcaa tcgggaagag cagaccgccc agaataaggc catagttgta
     9241 tacggacggc atcatgcaca acagtttttt ggagatatcc gcagcatcca gagtcgggaa
     9301 gacccaaacc tcacttggct gaagcatgga gttgcggtgc acgcgcacgc tgtaggaatc
     9361 tgacgacaga gcggcatcaa tctggatttc cccctctatg gagatttcat tgttcaggca
     9421 atccttctgc accatgctct tcgccagcgc cgcagcggcc ctggtgcggt ccgcagccag
     9481 ttccggaacg gagcccgaag tggacgcgct cagcatggcg acgcgaacgc ttttacccag
     9541 catgtggcgg gccattttgg ccgtctctac ggcaaaatag gccatatttt ccaccgtggg
     9601 ttcagggttt accccggtat ccgccagaaa atagacgccg cggccgccga atttcttgga
     9661 aaattcggga acgagcacaa tggaaatggc aaacaggggc ttggtgggaa cgggatcctg
     9721 cctgtacttg tttacccttt tcatgttccc ggccacaatg gcgtccgcct ggccgtagag
     9781 caccatcatg gcggcaaaat gaaccggttc ggaaacaatt tcacgggtat tcataggcaa
     9841 cccgttgccg aacatctgct ccgcccgttc ataacggtcg cagaacatct gaagatccga
     9901 gcttttttcc ggttcaatga tacggacgaa tttcagggaa attccgttca tttccgccat
     9961 tctcgtgatg acttccctcc tccccagcag gataggcgcg ccggcctgct cttccacaaa
    10021 gcgctgggac acgcgcagca cgcggacatc ctccccttcc ggaaaaacaa tgcgcttggg
    10081 gtggcgcttc aggttatcca ggagcggccc tgtaaaatca ttcagcgggg aaaagccttg
    10141 ccagtcactc atatgtcacg tgtcctattt aacgcaactc taaaaaatcc tgtccataaa
    10201 atcaactcat aaagtcaaag acggcaaata atgaacaaag acatccctcc gggttgaacc
    10261 gtccgctccg ccatgctagc atgggcattc ccctccatgt actgcgatta ccatacccat
    10321 acccccctgt gcctgcacgc ctccggcact ccgcaggaat atgtgcaggc agccgtccga
    10381 gccggactcc gggaatacgg catttcagac catgccccga tgcctgggga accttttgac
    10441 gactggcgca tgaagcaggc ggacatgccg gcctacctgg actggctcac ggaagcccgt
    10501 gaatgcgccg cgctgcacgg actgacggtc cgttccgctc tggaatgcga ctggtttccc
    10561 ggcatcgaac catggataga gcacctgcag ggcctgcacg catgggatta cctgatcggt
    10621 tccgtccatt atcttgggga aaaggaggaa ttcgacaatc cccacaggat ggatttctgg
    10681 caccggacgg atgtggagga cgcatgggaa cagtattggg aacgcttccg gaacatggcc
    10741 gcctccggaa tgttccacat catggggcac gcggacctga ttaaaaaatt cggcttccgc
    10801 ccttctggag acctgcgccg ttattatgaa ccgtcgctgg aagccatgcg ggaaaccgga
    10861 gcctgcctgg aacttaacac agccggttgg cacaacaagt gcggggaaca atatccggac
    10921 gggcaattcc tgaaaatggc ggcggaggcg ggaattcccc tcaccatcag ttcagacgcc
    10981 cacaggccgg aggatgtagg gcgggatttc aagcgggccg gggaactggc ccggcaggca
    11041 gggttcaggc atctggcctc cttccagggg ggccgcatga aactgttccc cctgcccgga
    11101 atctgatgct ttccctctgc ggcaaaggaa tgttccggca ggccatggcg gcagccgcac
    11161 gggcggcctg tttcccgtat tcctttccgg cagtcccttt ttccagtaaa cggcacaggc
    11221 ccatggacag aaaagggaat gctccggagc atctttaact tctgtacaaa ctcggcttta
    11281 cgggcaaagg catcggacgc ggaggcacgg gacggggagg gggcggttgg ggccggataa
    11341 agacaggcgg cggcggataa ttccacgtcc tgataatcgg agggacaatg atgcgttccg
    11401 ttctcggctg gctggccacc tccctcactt ccccctgcaa ttgggaaacc tgcttattca
    11461 aatccgcaat cttcttctga gtaccgtctt tcaaggccag gtatttcttc tccaaatcct
    11521 gccaatactg ttccgcggtt tccctttttt tacggatggc gcttatctgc tcctgatagg
    11581 ctgccgccct tgcgcggaaa acgcgggcgt cttccggcaa acccacacgg tccgcttcca
    11641 gggcattcca ctgcagagcc ataacctccg cctccacctt ggtaagttcc cccatcagca
    11701 aaaacatgcg ggaaatatcc gccccttcca ggtagcgggg atgccgctcc gcctcagaca
    11761 gggactcccg gatcttcctg gcttcttcat cggccgcctt cctggcttct tccgccttct
    11821 ttctgcgcgc cgcttcctcc atccttcgct ggtccagcct ggccttcact tcatccggcg
    11881 tcatcctgta atccctttgc tgggcatccg tcaaatcttt ataggaaacc ttggccaccc
    11941 cgtccgtgta ctggatattg acaaaatcat ctccggaaga cactacaacg gcgtctttca
    12001 gcaccctgcc ggaccgcagg gacatatttt ccgccagggc ggcaggagcc aggaggcata
    12061 aaatcaacaa acgggttcct gttttcatat gattaacctc ttctacgttg gtaagaggcg
    12121 caaggaaagg aatttattca aaaaaatagg accagaggtt aatactggcg cagccgccgg
    12181 tttttagcta gtatatggcg agtcatttac acgtattatg agccgctatt cttcatcctg
    12241 gacaccttta cccgcccttc ccgatccgga aggcttcgcc ggcatgtacg cagggacctg
    12301 cggagaaata atgatttgtg caggaggtac caatttcccg gaaaagccca tgcttgaagg
    12361 gggagccaaa acgtggacgg accgtatctt tacgctctct ccgggggaaa acgaatggaa
    12421 agaggccggc acattgcccg cgccttatgc atacggcgcc tccgccggca tcagggaagg
    12481 cctgctgtgc atcggcggct gcggcaaaga agggcaccgg aaagacgttt acctgctgaa
    12541 cctcgcggag ggaactgtca ccacgcatcc tttcccttcc ctccccatcg ccctggcata
    12601 tacggcggct gccgtcctgg acgggaaagt ctatcttaca ggagggtgcg aacgccccgg
    12661 cgagcaggac tgcactaacc gggtcttcat gctggacaca cgccggacgg aacagggatg
    12721 gctggaacag gccccgctgc cgggccgcgg ccgcttcctg catcagatgg ccgcaacaga
    12781 cggagccctg tacgtgctcg gcggcatcgg cctgcgggaa caggacggcc aacaggtccg
    12841 ggaactgctg aaggaagcct ggagctatac tccggaacac ggatggacgc gcctgccgga
    12901 catgccttac gccatcgccg cagcccccac cccggccccg gtttccggaa acggcgtcat
    12961 ttacctgctt gggggggatg acggctccgg aaaaaaatac actccccaaa ctaatcccgg
    13021 cttcaataac caatccctgt gcctagacac ggcagacatg acatggcacg acgcaggccc
    13081 catcgccgcg cccagagctg tccttccgtg ttgcgcgtgg caggaccgtt tcgcggccgt
    13141 gaacggagag ctcaaacccg gcaggcgctc tcctgaggta tggagcatct ccctcccctg
    13201 atttgaccgg aatccaatta aacctctcaa catatgcaca tgacacgcac ccttacccga
    13261 atttccaagg cttgcggctc tgctctggca gccctgcttc tcatcgcggc tccggccgct
    13321 gacgcatcct tttccgtgga tgtccggacg tccggaactg ttcccgacct tccggacccg
    13381 gcaggccgcg ccggcatggt tgccggaacc gtcacggaag acgacggcag ccagtccgtc
    13441 atcgccgcag ggggcgccaa cttcccccag gctgcgccgg gcgcctcaac tccggaggaa
    13501 cgcggcccca aaacctacta ccaggatatt ttcaagcttc gcaacggcca gtggagcaag
    13561 gcgggaacat tgcccgtccc gctgggttac gccgcctttg ccagcgtggg caaggggctg
    13621 gctgtggccg gaggccacaa tgctcagggc atcctgaaag atgccctgct gattaaaacg
    13681 gacggctccg tagaaaagct tcctcccctt cccgtccccg taacggaagc ctcctgcgcc
    13741 gcccaaggga acaaattgtt cgtcatcggc ggcagagacc gggagcagcc ggaaaccgca
    13801 ctcaatacca tctacatgct ggacaccacg ccggacacgg ataaaatgaa atgggtttcc
    13861 cttccccctt ttcccggaga ggggcgcatt ctctccaccg ctgccgtctg cgacagcacc
    13921 ctgttcatca taggcggctg ttccctgtcc agggataact ccggagaaac ctccaggacg
    13981 tatctttccg acatgatcgg ctacgacatg acgtccaagg acccttccaa atggggctcc
    14041 tccggcaggc agcagcttgc cggaccggga atgcccgtag ccgccgccgc gggtcccgcc
    14101 ccggtgcggg aaaactccat tctcctgatc ggcggagaca agcgcggcaa ctcgccggac
    14161 ccgtccaggc ctgtggcgca gtcccgggat atcctggtct atgacgtcat cggaaacaca
    14221 tggacgcgcc agggggaatg gcccgtgggc atcgccacgg ttcccgccgt tgtcaggggc
    14281 tctgaaatca tgaccatcag cggggaaacg gccccgggcg tccggactcc cgtaaacgcc
    14341 tccgcctccg caggttacca ctttgaaatg agcacggttg attacgccgt tctcattctg
    14401 accatcatcg tcctggcgat catcatcgtc tccgccgtgc gcaacggcgt taaaaatgtt
    14461 gcctccgtca cggacccgaa caccaagccg ggcctgtggg cctgggtagc cgtcattgtc
    14521 ctgtggtttg tggtcatgct gaactatttt gaccgccagc tcctttccgc cctgcatgaa
    14581 cccatcgtac gggacattcc gcagacggaa gcccagttcg gcatggtcac ctccgttttc
    14641 ctgctgattt acgccctgct cagcccggtg ggcggctttc tggcggaccg ctacagccgc
    14701 cggctgatga tcctctgctc tctcgtcgtc tggtccgtgg tcacatggtg gaccggccat
    14761 gcggaagatt acacttccct gctcattgcg cgcggagcaa tgggcatcag cgaggcattc
    14821 tacatccccg ccgccctggc cctgattacg gactaccacc gcggcagcac gcgctccatc
    14881 gctacgggcc tgcacatgag cggcatctat gtaggcatgg ccgtcgccgg tttcggtgcc
    14941 accatggctt catggacggg atggcgcatg acattcgccc tgttcggcct catcggcgtg
    15001 gcgtacgccg tcatcctcat cctgttcctg aaagatccgt ccaaagcccc ggcggatacg
    15061 gcccaggcaa aaaaaccgtc cgtaccggag aaaaaaacgg tgctgctcaa tgtggacaat
    15121 gacgaacagg ccatcaagga accctcttcc aaactttcca ccggagccgt cctttccagc
    15181 cttctgagcg gacgccccat gtggatgcta ctggccgtag tggcctttgc cggagccgga
    15241 aactggttcc ttctcacctg gtatcccacc ctgcttcagg acaaatacca gctctcctcc
    15301 gcggaagcgg gccccgccgc caccctgtgg agctccgtgg ccaaatacgt cgccgtgctg
    15361 ggaggcgcca tcctggcgga catgtggtac aggcgcaacg cccgcgcccg cgccctggtg
    15421 ccgggcatca ccttcaccat atccggccca ttggtggttc tggcgctgct gcccggcatc
    15481 ttcggctggg acatcaccgt cccgctcgtg ctcatgctgg gtctggtcgc cacgcagggg
    15541 ctggcgcagg gatcactgga cgccaccctc atgcctgtgc tacgctccca tatcgacgag
    15601 cgttattccg caaccggcta tggcctcctc aacctcacat ccgccggcgt gggcgccctc
    15661 atctccttct ttgggggatg gttcaaggat cagggcgttc cgctgaccac caccctggcg
    15721 gcggcaggat gcctgatgct gttctgcggg ctgctgctcc tgatgctgcc gcgtcccaaa
    15781 cattaaacgc aattatgttc atgcctcccg tcttacccct gatgaaaaag cccgcacgcc
    15841 tctgcctggc ggtgggaact ctgttcgccc ttttccttac cccccttgca caggggcgca
    15901 cctggaccaa cctccaaggg aaaaaactgg aagcagaatt catcaggctg gacggccaaa
    15961 aagccgtgct gaaacgttcc ggcggccaaa ccgtctccat tcccctctcc cagctctccc
    16021 gggaagacag ggatttcatc gcggaacagg aaaaaggtgg ggcactcccc tccaatacgg
    16081 cggacaatta ccacctgccg tggcccagga gcgtcaaatg cccggacaat ttcaaggtgg
    16141 aaaccatcaa ggaggaaccg ggagaatata tttatgaaac a
//
LOCUS       DAOMSU010000020        16141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_129_length_16141_cov_3.127316, whole genome shotgun sequence.
ACCESSION   DAOMSU010000020 DAOMSU010000000
VERSION     DAOMSU010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 16141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16141
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_129_length_16141_cov_3.127316"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="PK077_01795"
     CDS             complement(<1..1173)
                     /locus_tag="PK077_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN23063.1"
                     /translation="MLQANGITYEIETPDGPLKLLDNVSFNVPRGHFMAVVGPSGCGK
                     TTLLKAIAGMIAETGGRFFWNGHDLAEEDFEPSEIGFVPQFSIAYDQLSVDENVESAA
                     RLRCRFNSVDDLDDSIDNALEVTGMEGITDRDVKILSGGQKRRLALAMELVSNPRLLI
                     CDEVTSGLDPRSEHDIVFLLHEISRSEGRIVISVTHSLSHLDRYDSILVMHQGCVAYH
                     GSPKTMLHYFGVSSLEEIYPKLQDREGPSWSRSWSKHRDSYYSRLEQEREKKILSGEL
                     PDPDAVRLTEAEKEGASGESGTEREKAVEENIPEVPGFFTQFFCLLGRRWRIFFRDRS
                     QLVLQLVMVLLFPVLVAMFTDKGSGQIVGLSATQDVQTVQKDMEAQQLNMKTGSAVS"
     gene            complement(1480..3237)
                     /locus_tag="PK077_01800"
     CDS             complement(1480..3237)
                     /locus_tag="PK077_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRN23064.1"
                     /translation="MSVIKAFLVSPLKWLRVTVAVIFFIGIAYAFLHHIPAWNVPNMT
                     GGGEGVEDSFSLARWQLVPSVLGTLSGAAVSAVILGVLLLLTLLFGRVYCSFICPLGI
                     LQDIVFRIRRWLAPKRFLKFSRPVPWMRYGVLALLAACCVTGLAGLSLNWLDPYSIFG
                     RIMYVLAWPAAIWSNNLLAADSSSADLVQMDYFPAAFPVLLASAGMLGLVAVMSAWKG
                     RLYCNTVCPVGTFLGLLSRISLFRLGFDPASCKKCGKCVKSCKAQCLNLKEYKIDSSR
                     CVACYDCVRSCSEGGIRYRWFTRVRQQIPAQKKKVPPASAPSSSAAVPSIAGSSRRQF
                     LEATAVGLAAAAFSGCRGDAARKLDPTQCVLPPGAGSLERFLDICTGCQMCVANCPTH
                     VLQPSYLQLGLKGFMKPRMDFATKYCLYDCHRCAEVCPTGAIRRMPVTAERDTEGITK
                     DTTRIAVARFYVCRCLVAREDMDCGACTEHCPTKALYTVPYIGRDGQEHRLPRLDPSL
                     CIGCGACEHACPVTAERPEERERRNSCDLCEEKCEFGRRREMPPRALVVRAVNPQQKA
                     VKKFEDKAVDPVGDADFPF"
     gene            complement(3315..4229)
                     /locus_tag="PK077_01805"
     CDS             complement(3315..4229)
                     /locus_tag="PK077_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRN23065.1"
                     /translation="MTDRRTFLKRTAAAGALAAISDMDSLFAQEAGAPAASGIPDLVA
                     VRNGTRAEMVRKAVETLGGMQAFVKPGQTVVIKPNIGWNKAPEFGANTHPETVATLVE
                     LCRQAGAKEVRVFDHCCHNGAYEGSGVKEAVEKAGGVMVDGGNESQYVQTENPKARKF
                     TSAKVHKAVLEADVYITCPPLKHHSGSEMTACMKNVMGTVWDRGAMHKNDLHQCIADA
                     VYFRKPDLCVLDAYMPMVRNGPVGKDTNDLVERKTLLASRDIVAIDAAGAALLNKTGK
                     IRHVQLGEEMGLGVADLSRLHIRRISMA"
     gene            4393..4968
                     /locus_tag="PK077_01810"
     CDS             4393..4968
                     /locus_tag="PK077_01810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRN23066.1"
                     /translation="MKCSLLSRPMTCAHDWISRVVFPGDAVVDATAGNGHDTVFLARL
                     AGPSGQVHAFDVQEEAIRATRERLEKEGLLTPSVRLHLASHDCLAELVRSPVKAIVFN
                     LGYLPGGDKKTVTRTECTLAALEQAAALIAPNGLLSVMCYPGHEGGKEEAEAVEAFLS
                     RLPHHSWRAGKYQLLNTGTPAPFQICAFRLD"
     gene            complement(5001..5645)
                     /locus_tag="PK077_01815"
     CDS             complement(5001..5645)
                     /locus_tag="PK077_01815"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRN23067.1"
                     /translation="MIFNCKRLMIMEQELPLLVVATRNAHKTGEIRAMLAGKWEVKDL
                     SDYPQAPPVDETGVTFTENATLKALSASKCIPGVLLADDSGLEVDVLDGRPGVWSSSF
                     GGEEGNHARNNLRMMEELRRAGVKPGDRPSARFRCVMVLAGNGRVLAEFSGSVEGYML
                     TEPAGEGGFGYDPLFVPEGYDRSFAQLPMEVKNSMSHRARALAQVVEWMKERRA"
     gene            complement(5726..6022)
                     /locus_tag="PK077_01820"
                     /pseudo
     CDS             complement(5726..6022)
                     /locus_tag="PK077_01820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
     assembly_gap    6023..6032
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6033..6420)
                     /locus_tag="PK077_01825"
     CDS             complement(<6033..6420)
                     /locus_tag="PK077_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HRN23068.1"
                     /translation="MKLVSWNVNGLRAVLGKGMADAVDALEPDVLCLQEIKARPEQVK
                     DLWISSWPHQLWNPAEKAGYSGVLILSRVRPLSTSVGIGWPEHDREGRVCTMEFEGFY
                     LVNCYTPNSQNELVRLPYREQWDAAFR"
     gene            6675..7970
                     /gene="nusA"
                     /locus_tag="PK077_01830"
     CDS             6675..7970
                     /gene="nusA"
                     /locus_tag="PK077_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRN23069.1"
                     /translation="MTNDIKALIDYYEREKGLSREKILLALESAFLSAYRKMVPGSGS
                     INYLRAEINVDKGKVRIFADLEVVPDEEYSDKFNQIPLSLAVKLDKNAVLHDLLPTNI
                     TPKGFGRIAVQTARQTMLQKLLDAEKEMLYDEFKDRAGDLVTGTIRRFEKGDIFVDLG
                     KFEGVMTSRERVPNEDYSVGDRMRFYVVEVRTEARGPEVILSRSHPNLVRRLFESEVV
                     EIGDQTVEIHGIAREAGYRTKVAVISHDDKVDPVGACVGMRGARVKNIVRELNNEKVD
                     ILEWTEDPVTFVREALSPVEPREITVDEEARKIFVIVQDDKDLSKAIGRRGQNARLTS
                     RLMGWDVQVRVFDVQEAEKRQSQAAAEEVMRQCQAAAKTLSEQLEIPEETAMGLVTMG
                     GTDLVALTGFEASDIAESMGIPAEEAAQILDKARDLISQ"
     gene            8013..10064
                     /gene="infB"
                     /locus_tag="PK077_01835"
     CDS             8013..10064
                     /gene="infB"
                     /locus_tag="PK077_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRN23070.1"
                     /translation="MPNKQEEKEPKKEVLDLIGGSSKKKRAPQPAPAPAPSRPAPVKK
                     EALDLLSGNKKKASRAADAAPAPAAAPAAAEPAAPAPQEEPSADDKIINLKPPVSVSE
                     LAGMLKAKPFQIIKDLMGMGIFANPNTPLDADAVSSICDLHGYTFAREKREKGGGVKA
                     QQEPVKEPEPVPVVEEPKATLITRTPIITVMGHVDHGKTSLLDYIRKTRVAKGEAGGI
                     TQHIGAYTVDYNGSTLTFLDTPGHAIFTEMRARGADVTDIVVLVVAANDGIMPQTREA
                     IAHSKAAGKTIIVAINKCDLPAADPVKTKSGLMEEGLVPTDFGGDVECVEVSALTGAG
                     IDDLLGLLVLQSEVLELQANPKANCRASIIEARVEPGTGSSATAIVESGTIRVGMPFI
                     CGPYAGKVRALVNDHGERVKKVGPGMPVEITGFSETPNVGDELVEMENERAAKKLGEE
                     RQEELRKQRLAQPRKARMEELLAMMGDGTQKAQLKILLKGDVQGSVEAIRKAVLDIQS
                     DKVECVFLNASAGPISESDVLLASSSDAVILGFNVKVEANAVKLLKREGVQVKLYSIV
                     YELIDQVRDAMLGLLEPETRETIIGHAKVLQVFKLNKGRAAGCMVEDGKILRSCEARV
                     IRDKTPVFDGKMSTLRRFQDEVEEVKAGLECGIRLGDFNEYETGDIIECYTLEKIQQT
                     L"
     gene            10206..10394
                     /locus_tag="PK077_01840"
     CDS             10206..10394
                     /locus_tag="PK077_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRN23071.1"
                     /translation="MSRRTDKVNELLRREIGTTIQRDFEFPGTIVTVIEVEVTDDLKE
                     GKVWVGVVGKMAPRCWKN"
     gene            10379..10579
                     /locus_tag="PK077_01845"
     CDS             10379..10579
                     /locus_tag="PK077_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRN23072.1"
                     /translation="MLEKLSSRHGLIQSAVARRVVLRNTPRLTFRLDDSAQRGVDLVN
                     LLEDIDKNLPKAPPADAENDGE"
     gene            complement(10773..15017)
                     /locus_tag="PK077_01850"
     CDS             complement(10773..15017)
                     /locus_tag="PK077_01850"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRN23073.1"
                     /translation="MFPHRFFTVLLFFLLFLAPAGYADVEYVWRGADYQWGSLSNWSV
                     GGVAASSAPGAAASAYEHWMVTNGTDSAGRTDVGGLGAGGRYLKGVRIEGLNSQPEGK
                     IPLFIKNTNKDVYLRVEEGGITVENAGEGGYSADFGVAQLRVAADQEWHVAEGRSLYV
                     GHDDDAPSGGLYSLTSEGDVPRRVTVTGGGAVRIGEGMLLNNISGLIGFVLNAGKGIP
                     TLDLADRGMGNTVTVEDAARLEGMSLYQGALVTRENASVTFSGTEAKASGQWNIGADT
                     ELALENSTLDLTEAGVDGNVILSGSSGITGDKGTLRQTLLDDARVTYTDRNVKAGEIR
                     SVGSNVTITLNNSSMHFDGEIPAVDLVVQGSCALGGSGAFSGTITYAPGGTLTVDGDI
                     SLPSSSLTLGRLHVATLDGVPQNSAVRPETPAQQAREYVVLFDSSFEDLISEWPGRHT
                     LGVQFRPDMGVPVTVKELPEGAVSWNYDAASGWLTLQTDVAEKPDMPAHVSGARPNII
                     FVLVDDMGWGDMSVNWTQQDKNGRQVTRRNEFKTPTLDTMAAEGMQLRRHYSAAPVCA
                     PARASLLLGVHQGHSRVVRNNTFDYPIENSHTLGTLLKSAGYHTAAIGKWGVGGGGQS
                     GKALTAAPHMRGFDYFYGIIKHLAGHYHYLTAGSQDIYEYNDQAAAPAWESVSAKVPG
                     TAYDTDLFGARAKQWIVDHHEKTPSRPFFLYLAFPAPHGSLSAPACAYPAGLGRDGGL
                     QWEKKDGYEACNTAADGWSGVEGDFSPDTYIYPEHEVFGRTESRHATMIRRVDDVLKD
                     MIQLLKDLGIDDNTMIVFTSDNGPHNEPGSGNYGNGAQDPAYFMSYGMMDGIKRDCWE
                     GGMRVPAVVRWPGVVPSGISLNASQFQDWMATFADVAGVPVPSRCDGVSLLPTLAGVP
                     ERQKTGVIYAEYAYSGSTPNYSDFLQQHRGRGRQQQQIIFVDGFKGIRMGNAAPATDF
                     EIYDTEKDPQEAANLAASRPDLQEKMKAQALRSRRSSPIASTSFDAGYIAPVSAPAGL
                     REGRLCWRAWNRSFDWVPDFRQLEEGPCSTGATDVSDVLSVKAGLSGQKGVELTGYLR
                     VPVTGEYQFYLQTDSNEGSRAFVHLHDMQLIDADYAYTPGTQAASNAREGVSGDVQPN
                     AVQTVKLTAGLHPIRIGYVGKAASSSLSLQWEGPETSGREPVPASAFSYEYVNPFNLE
                     KTEETVGCAASGTGLTVQTHLPWTVSCDQPWVTVSPVSGSGTSVLDIAVEANGLQTER
                     EAVVTVVCGGEQRTFTLRQEAAPAPAGYDKWKQDHFADGTPDEQMAPDACPAGDGISN
                     LMKYATGMDPNQPCGSVTKLAVREEAGGKYLVLSWPVNPEATDVTFHVESSSDLEEWA
                     DEGAVTPDGACGEFRDTVALGKGSPERRFLRLKVTR"
     gene            complement(15200..15958)
                     /locus_tag="PK077_01855"
     CDS             complement(15200..15958)
                     /locus_tag="PK077_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN23074.1"
                     /translation="MKKTLIIIASLALGAAASQAAVLCTTTFNRTGTSLDSVTVSTDS
                     DAGLSTSTSISSDNFSKNISGNDLLANGTIPASVFSPNANVGNNQNNTWRVSFTFTNG
                     GTQDMLISSIDLSMIGFTGAGAAQNAGGGVANNNYVGGADGNLNKPVNITLGMSGQDN
                     QMQAYNGATSTNTSTGSWDGVHTGSYKYGDVLLKAGESMTLTVTASKNEAYGSGCFIG
                     LTGIQIKGEVVPEPATASLGLLGLAALMMRRRRR"
BASE COUNT         3576 a         5143 c         4383 g         3029 t
ORIGIN      
        1 ggagacggcg gaccccgttt tcatgttcag ctgctgggct tccatatcct tctggacagt
       61 ctggacatcc tgcgtggcgg aaagccccac gatttgcccg gagcccttgt ccgtaaacat
      121 ggctaccagc acgggaaaca gcagcaccat gaccaattga agaaccaact gggaacggtc
      181 gcggaaaaag atgcgccagc gccgccccag caggcaaaag aactgggtga aaaatccggg
      241 aacttccgga atgttttcct ccacggcctt ttcccgttcc gttccggatt ctccggaggc
      301 cccctccttc tccgcctccg taagacggac ggcatccgga tccggcagtt ccccggaaag
      361 aattttcttt tccctttcct gttccagacg ggaataatag gaatcccggt gcttgctcca
      421 ggaccggctc caggaagggc cttcacgatc ctggagcttc gggtaaatct cctccagcga
      481 ggaaacgccg aagtaatgca gcatggtttt cggcgaaccg tggtaagcca cgcagccctg
      541 gtgcatgacc aggatggaat catacctgtc cagatgggaa aggctgtgcg tgacggaaat
      601 gacgatgcgg ccttccgaac gggagatttc atgcaggagg aacacgatat cgtgttccga
      661 cctgggatcc agtccggacg tcacctcatc gcaaatcagc agccggggat tggataccag
      721 ttccatggcc agcgccagtc gccttttctg gccgccggaa agaattttca catcccggtc
      781 cgtaatcccc tccattcccg ttacctccag ggcattgtca atgctgtcgt ccaaatcgtc
      841 cacggaattg aaccggcaac ggagcctggc ggcgctctcc acattctcat ccacgctcag
      901 ctggtcatag gcaatgctga actggggaac gaacccgatt tccgaaggtt caaagtcttc
      961 ttcagccaaa tcatggccat tccagaaaaa acgcccgccc gtttccgcaa tcatgcccgc
     1021 aatcgccttc aataaggtcg tctttccgca gccggaagga ccaacgaccg ccataaaatg
     1081 accgcgaggg acattaaaac tgacattgtc caataatttc aaaggaccat ccggtgtttc
     1141 tatctcatac gtaatgccgt tcgcctgcaa catggttctt ttactttaac gtatcaactt
     1201 ccgcatcatc aaggaaaatc gggcatttgc actctatccc gccggaaacg gagaacatgc
     1261 tctccgggaa ccttccccgc cagccgcggg aacgctcccg cggtcaggct cctcctgccc
     1321 ccgctcattt cggcatcccc cgcggccgta tccgtgccgc aagccgggct gcacggaatt
     1381 cccccgaaaa accggatgga cagacacagc cctcccgaaa gggaagtttg aagcctccgg
     1441 gagcgtttat ttcatcatct tccacaccgc cagccgtttt taaaacggaa aatccgcatc
     1501 ccccaccgga tccacagctt tgtcctcaaa ctttttaaca gctttctgct ggggatttac
     1561 ggcacggacc accagcgccc gggggggcat ttcccgccgc cttccgaact cgcatttttc
     1621 ctcgcacaga tcgcaggagt tccggcgttc gcgttcctca ggccgttccg cggtaacggg
     1681 acaggcgtgt tcgcacgcgc cgcagccgat gcacagggaa ggatccagcc tgggcaggcg
     1741 gtgctcctgg ccgtcacgcc cgatataggg caccgtgtaa agggccttgg tggggcaatg
     1801 ctccgtacag gctccgcaat ccatgtcctc ccgggcaacc aggcagcggc aaacgtaaaa
     1861 ccgggccacg gcaatgcgcg tagtatcttt tgttattccc tcagtatccc tttccgcggt
     1921 aacaggcatc ctgcgtatgg cccccgtggg gcagacttcc gcacagcggt ggcaatcata
     1981 aaggcaatat ttggtggcaa aatccatccg gggtttcatg aatcccttca ggccaagctg
     2041 caggtatgag ggctgaagca catgggtggg acagttggca acgcacatct ggcagccggt
     2101 gcagatatcc agaaaacgtt ccagggaacc ggctccggga ggaagaacgc actgggtagg
     2161 gtccagcttc cgggcggcat ctccccggca tccggaaaag gcggccgcag ccagcccgac
     2221 cgcagtagcc tccaggaact ggcggcggga actcccggca atggagggaa ccgcggcgga
     2281 agaagaggga gcggaagccg ggggaacctt ctttttttgg gccggaatct gttggcggac
     2341 cctggtaaac cagcggtagc gtattcctcc ctcactgcag gagcgcacgc aatcgtagca
     2401 cgccacgcaa cgtgaggagt ctattttgta ttccttcagg ttcaggcatt gcgccttgca
     2461 ggatttgacg catttgccgc attttttgca ggaggcggga tcaaagccca gccggaaaag
     2521 ggagatacgg gaaagcaacc caaggaacgt gccgacgggg cataccgtat tgcaatacag
     2581 gcgccccttc caggcgctca tgacggccac caggccgagc atgccggcgg aggccagcaa
     2641 taccgggaaa gccgcgggga aataatccat ttgaaccagg tccgcagaag aactgtccgc
     2701 cgccagcagg ttattgctcc aaatggcggc cggccatgcc agcacataca tgatgcgccc
     2761 gaaaatgctg taagggtcca gccaattcag agacagaccg gccagccccg tcacgcagca
     2821 ggcggccagc aatgccagca ccccataccg catccacggc acaggccggg agaatttcag
     2881 gaaacgcttc ggcgccagcc agcggcggat tctgaaaaca atatcctgga gaatccccag
     2941 cgggcagatg aaggaacagt atacgcggcc gaacagcagc gtaagcagca gcaaaacccc
     3001 cagaatcacg gcggaaacgg cggccccgct caaggtcccc agcacggacg gcaccaattg
     3061 ccaccgcgcc agggaaaagg aatcttccac cccctctccg ccccccgtca tgttcggcac
     3121 gttccacgcg ggaatgtggt gcagaaaagc gtatgcaatc ccaataaaga aaatgacggc
     3181 caccgtcacg cggagccact tcagaggact gacgagaaag gctttgatga ctgacatatt
     3241 tatcataaac ccgccggagg cggaaagttg gccctccacc gcgtcatccg cagacgggag
     3301 aaggagccgc cgcgtcaggc catgctgata cggcggatat gaagcctgga aaggtcggcg
     3361 acgcccagcc ccatttcctc cccaagctgc acatggcgaa tttttccggt cttgttcagc
     3421 agggcggcgc ccgcagcgtc aatggccacg atgtcacggg aagccagaag ggttttgcgc
     3481 tccaccagat cattcgtatc cttccccacg gggccattgc gcaccatggg catgtaagcg
     3541 tccagcacgc acaggtccgg cttgcggaaa tacacggcat ccgcgatgca ctggtgcagg
     3601 tcgttcttat gcatggcccc gcggtcccag acggtgccca tgacattctt catgcaggcg
     3661 gtcatctcgg aaccgctgtg gtgcttgagg ggcgggcagg tgatgtaaac atccgcctcc
     3721 agcacggcct tatgcacctt ggcggaggtg aatttccggg ctttcgggtt ttccgtctgc
     3781 acgtactggc tctcattgcc gccgtccacc attacgccgc cggccttctc tacggcctct
     3841 ttcacgccgc tgccctcata ggcgccgttg tggcagcagt ggtcaaacac ccttacttcc
     3901 ttcgctccgg cctgcctgca cagctccacc agcgtggcga cggtttcagg atgtgtgttg
     3961 gcgccgaatt ccggagcctt gttccagccg atgttcggct tgattacgac ggtctggccc
     4021 ggcttgacga aggcctgcat gccgcccagc gtctccacgg ccttcctgac catctccgca
     4081 cgggtgccgt tgcggacggc caccagatcc gggatgccgg aagccgccgg agctccggcc
     4141 tcctgggcga acagggaatc catgtccgat atggccgcca gcgcccctgc ggcggcagtc
     4201 cttttcaaaa aagtgcgtct gtcagtcatc ttcatgccaa cgtctgtggt ttatccccgc
     4261 atgcgggaac acgcagaagg ttttatgcct cttcctaaca catgccctgc caaaaaacaa
     4321 cctgtcattc ttcccgcacc ggaatttcat aaacgaattg tcatacaggc agagcttctg
     4381 tacaacccgg tcatgaaatg ttccctgctc agccgcccca tgacctgcgc tcatgactgg
     4441 atcagccgtg tcgtcttccc cggggacgca gtcgtggacg ccacggcggg caacgggcat
     4501 gatacggttt tcctggcccg gctggctggc ccttccggcc aggtgcacgc ctttgacgtg
     4561 caggaagaag ccatccgcgc caccagggag cgtctggaga aggaagggct gctcacccct
     4621 tccgtccggc tccatcttgc cagccatgac tgcctggcgg aactggtcag aagccccgtg
     4681 aaggccatcg tcttcaacct gggctatctg ccgggcgggg acaaaaaaac ggtcaccagg
     4741 acggaatgca ccctggccgc gctggaacag gccgccgccc tcattgcccc gaacgggctt
     4801 ctcagcgtca tgtgctatcc cggccacgaa ggaggaaagg aagaagcgga agccgtggaa
     4861 gccttcctgt cccgcctgcc ccatcattcc tggcgcgccg gcaaatacca gctcctgaac
     4921 accggaacac cggcaccctt ccagatatgc gccttcaggc tggactgacc tccccccccg
     4981 ccaggcgccc gggaaaaacc tcaggcccgg cgttccttca tccactccac cacctgcgcc
     5041 agagcgcggg cgcggtgcga catggaattt ttcacttcca tgggaagctg ggcgaaactc
     5101 cggtcatacc cttccgggac aaacagcgga tcatagccga accctccttc tccggccggc
     5161 tccgtcaaca tgtagccttc cacggaaccg gaaaactccg ccagcacgcg gccgttcccg
     5221 gcaagcacca tgacgcagcg gaaccgcgcg gaaggcctgt cccccggctt cacgccggcg
     5281 cgccgcagtt cctccatcat gcggaggtta ttccgcgcat ggttcccttc ctccccgccg
     5341 aaggaggagg accagactcc ggggcgcccg tccagcacat ccacctccag cccggaatca
     5401 tccgccagca ggacgccggg gatgcatttg gaagcggaaa gcgccttgag cgtggcgttc
     5461 tccgtaaacg tgactcccgt ttcatccacg ggcggagcct ggggatagtc ggaaagatcc
     5521 ttcacttccc atttcccggc cagcatggcg cggatttccc cggtcttgtg ggcgttgcgc
     5581 gtggcaacca ccagcagcgg caattcctgt tccataatca tcaatctttt acagttgaaa
     5641 atcatctgaa agaccgcgct cagcagtcaa tctccaggct gacggggcaa tggtcggacc
     5701 ccgtcacatc cggatggatg gcggcatcct tcaccctgga agccagcgga ttggaaacgc
     5761 agaaatagtc aatgcgccat ccgatattcc gggcgcgggc gcccgcgcgg taggaccacc
     5821 agctgtacca gcccggctcc tccggatgca gagcgcggaa cgtatccgta aaaccggcct
     5881 caagcagcag cgtgaacccg gcgcgctcct ggtcgctgaa cccggcggaa aaacggttct
     5941 ctttgggacg tgcaatgtca atctcctcat gggccacgtt caagtctccg cagaaaacga
     6001 ccggcttggt ttccgccagt ccnnnnnnnn nncgcggaac gcggcgtccc actgctcccg
     6061 gtacggaagg cgcaccagtt cattctgtga attgggcgta tagcagttga ccaggtaaaa
     6121 cccctcgaat tccatcgtgc agacgcgccc ttcccggtca tgctccggcc agccgattcc
     6181 gacggacgtg gaaagaggcc gcacccggct gaggatcagc acgccggaat agccggcctt
     6241 ctccgccgga ttccagagct ggtgcggcca ggaggaaatc cacaggtctt tcacctgttc
     6301 cggacgcgcc ttgatctcct gaaggcagag gacgtccggc tccagggcat ccacggcgtc
     6361 cgccattccc ttgcccagaa ccgcccgcaa tccgttcacg ttccatgaga cgagtttcat
     6421 gcgcgcatac ttcccgtttt tgacgggtcc gtcaagccct caaacaccgt tgtcccgccc
     6481 cctcccggag ggctccggaa aattcttctt ccggaatcat ccccagtctt ttgcatgccg
     6541 catagaacaa gcgttttttc ttgccaggac cgccccctca tgcttactta gataggcaaa
     6601 caccccgaga ggcactcgag agtgggtctg tcgacccgct cttttttgtc tatccatcac
     6661 actcctccac actcatgaca aacgatatta aagctttgat tgactactac gagagggaaa
     6721 aagggctctc ccgtgaaaaa attctcctcg ctctggagtc cgcctttctc tccgcctacc
     6781 gcaaaatggt tcccggttcc ggcagcatca actacctgag ggccgaaatc aacgtggaca
     6841 agggcaaggt gcgtattttt gcggacctgg aagtggtgcc ggatgaagaa tattccgaca
     6901 aattcaacca gatccccctt tccctggccg tcaagctgga caaaaacgcc gtgcttcacg
     6961 acctgctccc caccaacatc acgcccaagg gcttcggccg catcgcggtg cagaccgccc
     7021 gccagaccat gctccagaaa ctgctggatg cggaaaagga aatgctctac gacgagttca
     7081 aggaccgcgc cggagatctg gtaacgggca ccatccgccg ttttgaaaag ggggatattt
     7141 ttgtggacct cggcaaattc gaaggcgtca tgacctcccg cgaacgcgtg ccgaatgaag
     7201 actacagcgt cggcgaccgc atgcgcttct acgtggtgga agtgcgcacg gaagcacgcg
     7261 gcccggaagt catcctttcc cgcagccatc cgaacctggt gcgccgcctc tttgaatcgg
     7321 aagtggtgga aataggcgac cagaccgtgg aaatccacgg catcgcccgc gaagccggct
     7381 accgcaccaa agtggccgtc atcagccatg acgacaaagt agatccggta ggggcatgcg
     7441 taggcatgcg cggcgcccgc gtcaaaaaca tcgtccggga gctcaacaat gaaaaagtgg
     7501 acatcctgga atggacggaa gaccccgtta ccttcgtccg ggaagctctc agccccgtgg
     7561 aaccgcggga aatcaccgtg gacgaggaag ccagaaaaat cttcgttatc gtccaggacg
     7621 acaaagacct ctccaaggcc atcggccgca ggggccagaa tgcccgcctc acctcccgcc
     7681 tgatgggctg ggatgtccag gtgcgcgtct ttgacgtcca ggaagcggaa aaacgccaga
     7741 gccaggctgc ggccgaagaa gtcatgcgcc aatgccaggc tgcggccaaa accctcagcg
     7801 aacaattgga aatcccggaa gaaaccgcca tgggcctggt gaccatgggc ggaacggacc
     7861 tggtggccct caccggattt gaagcttccg acatcgcgga aagcatgggc attcccgcag
     7921 aggaagccgc ccaaattctg gacaaggccc gggaccttat ctcccaataa ccgttgagcg
     7981 cggcgctcaa catctaacaa ccttaaatca agatgcctaa taaacaagaa gagaaagaac
     8041 ccaaaaagga agtattggat ctgatcggcg gatcttccaa aaagaaacgc gcaccccagc
     8101 cggcccccgc accggctccc tcccgccctg ctccagtcaa gaaagaagct ctcgacttgc
     8161 tttccggcaa caagaaaaaa gcgtcgcgcg ccgctgacgc cgctccggcg cccgccgctg
     8221 ccccggccgc cgcagaaccc gccgcgcccg caccccagga agaaccctcc gcagacgata
     8281 aaatcatcaa cctcaagccg cccgtctccg tttccgagct ggcgggcatg ctgaaagcca
     8341 agcccttcca aatcatcaag gatctgatgg gcatgggcat cttcgccaac ccgaacacgc
     8401 cgctggatgc ggacgccgtc agttccatct gcgacctcca cggctacaca ttcgcccgtg
     8461 aaaaacggga aaagggcggc ggcgtgaaag cccagcagga acccgtcaag gaaccggaac
     8521 ccgtgccggt ggtggaagaa cccaaagcca ccctcatcac gcgcacgccc atcatcaccg
     8581 tcatgggcca tgtggaccac ggcaaaacat ccctgctgga ctacatccgc aaaacgcgcg
     8641 tggccaaggg ggaagccgga ggcattaccc agcacatcgg cgcctatacg gttgactaca
     8701 acggcagcac gctcaccttc ctggatacgc cgggccatgc catcttcaca gaaatgcgcg
     8761 cccgcggcgc ggacgtcacg gacatcgtgg ttctggtggt ggccgccaat gacggcatca
     8821 tgccccagac acgggaagcc atcgcccact ccaaggcggc cggcaaaacc atcatcgtcg
     8881 ccatcaacaa atgcgacctt ccggccgcag accccgtcaa gaccaagagc ggcctgatgg
     8941 aagaagggct ggttcccacg gacttcggag gcgatgtgga atgcgtggaa gtctccgccc
     9001 tgaccggggc cggcattgac gaccttctcg gccttctcgt cctccagtca gaagtgctgg
     9061 aactccaggc caaccccaag gccaactgcc gcgcctccat catcgaggcc cgcgtggaac
     9121 ccggcacggg cagctccgcc acggccatcg tggaaagcgg caccatccgc gtcgggatgc
     9181 ccttcatctg cggcccttac gccggcaagg tgcgcgccct ggtcaacgac cacggcgaac
     9241 gcgtcaaaaa ggtagggccc ggcatgcccg tggaaatcac cggcttctct gaaaccccga
     9301 acgtggggga cgaactggtg gaaatggaaa acgaacgcgc cgccaaaaag cttggggaag
     9361 aacgccagga ggaactgcgc aagcagcgcc tggcccaacc ccgcaaggca cgcatggaag
     9421 aactgctcgc catgatgggg gacggcaccc agaaagccca gctcaaaata ctcctgaagg
     9481 gggatgtgca gggttccgtg gaagccatca ggaaagctgt tctggacatc cagtcggaca
     9541 aagtggaatg cgtcttcctg aacgcctccg ccggccccat ctcggaatcg gacgttctgc
     9601 tggcctcctc ctcggacgcc gtcatcctgg gcttcaacgt caaggtggaa gccaacgccg
     9661 tcaaactgct caagcgggaa ggcgtgcagg taaaactgta ttccatcgtt tacgaactca
     9721 ttgaccaggt gcgggatgcc atgctgggcc ttctggaacc ggaaacgcgc gaaaccatca
     9781 tcggccacgc caaagtgctc caggtcttca agctcaacaa gggccgtgcg gcaggctgca
     9841 tggtggagga cgggaaaatc ctccgcagct gcgaggcgcg cgtcatccgc gacaagacgc
     9901 ccgtctttga cggtaaaatg tccaccctcc gccgcttcca ggatgaagtg gaagaagtca
     9961 aggctggtct ggaatgcggc atccgcctcg gagacttcaa cgaatacgaa acgggcgaca
    10021 tcatcgaatg ctacacgctg gaaaaaatcc agcaaacgct gtaaccccca acaaaaatca
    10081 tccaacaggg acggagctcc ttgcacgctc ctccgggcgt ccaggaaacc tccgtccctg
    10141 tcctttccct tattcctcct tctccgattg aaccaatcgg tcccttcatc actctatccc
    10201 ttcatatgag cagacgcact gacaaggtaa acgaactcct gcgccgggaa ataggcacca
    10261 ccattcagcg ggattttgaa tttcccggaa caatcgtcac cgtgattgaa gtggaagtga
    10321 cggacgatct gaaagagggg aaagtatggg tgggcgtcgt agggaaaatg gctcccaggt
    10381 gctggaaaaa ctgagctccc gtcacggcct tatccagtcc gccgtggcca ggcgcgtggt
    10441 gctgcgcaat acgccgcgcc tgaccttccg tctggacgac tccgcccagc gcggcgttga
    10501 cctggtcaac cttctggaag acattgacaa gaacctgccc aaagcccccc ctgccgatgc
    10561 ggaaaatgac ggggagtaat attcttcccc tggctaaggc acggtgtccc cggccgtacc
    10621 gcgctggatg cgccgggaca gcggagggcc ggaacacgca gaatcacgct tccggaaaaa
    10681 atccgtaacg ggcggcaagg cagcagggcc ggaaggtccc tgttcccgta aggaatcatc
    10741 atcgggaaac ggaaagagaa accggtgatt ccctatctcg tcacctttaa tctcagaaac
    10801 cggcgctccg gagagccttt tcccagcgcg accgtatcgc ggaattcccc gcaggccccg
    10861 tccggagtga cggccccttc gtcagcccat tcctccaggt cggaagaact ttccacatgg
    10921 aacgtcacat ccgtcgcctc cggattcaca ggccaggaaa gcacgaggta tttcccgccc
    10981 gcttcctcgc ggacagccag cttcgtcacg ctcccgcagg gctggttggg atccatgccc
    11041 gtagcatatt tcatcagatt ggaaattccg tctccggcgg ggcaggcatc cggggccatt
    11101 tgctcatccg gcgtcccatc cgcaaaatgg tcctgcttcc acttgtcgta ccctgccggg
    11161 gcaggagcag cttcctggcg cagcgtgaat gtcctctgct ccccgccgca tacgacggtg
    11221 acaacggctt cccgctccgt ttgcagcccg tttgcttcca cggcaatatc cagcactgag
    11281 gttccggaac cggaaacggg ggaaaccgta acccatggct ggtcgcagga aactgtccag
    11341 ggaagatgcg tctgcacggt cagccccgta ccggaggcgg cgcatcctac cgtttcctcc
    11401 gtcttttcca gattgaaggg attcacgtat tcataggaaa acgcgctggc gggaacgggt
    11461 tccctgccgc tggtttccgg cccctcccat tgcagggaaa gggaggagga agccgccttc
    11521 cccacatagc cgatgcggat gggatgcagc cccgcggtaa gcttcaccgt ctgaacggca
    11581 ttgggctgca catcgccgga aacgccttcc cgcgcgttgg aggcagcctg cgttccggga
    11641 gtataggcat aatccgcatc aatgagctgc atatcgtgca aatggacaaa agccctggag
    11701 ccttcattgg aatccgtctg aaggtaaaat tgatattccc cggtgacagg caccctgaga
    11761 tagcccgtca attccacccc cttctgaccg gaaagccccg ccttgacgct gagcacgtcg
    11821 gaaacatccg tcgcacccgt agaacagggc ccctcctcca gctgccggaa atccggcacc
    11881 cagtcaaaag aacggttcca cgcgcgccag cacagccttc cctcccgcag gccagcgggg
    11941 gcggaaacgg gcgcgatata accggcgtca aaactggtgg aggcaatggg ggaggaacgg
    12001 cgggaacgca gcgcctgcgc cttcatcttc tcctgcaggt ccggacggga tgccgccaga
    12061 ttcgccgctt cctgcgggtc tttttccgta tcgtagattt caaaatccgt agcgggggcg
    12121 gcattcccca tccgaatccc cttgaagccg tccacaaata taatctgctg ctgctgccgc
    12181 cctctccccc tgtgttgctg gaggaaatcg ctgtaattcg gagtgctccc gctataggca
    12241 tattccgcat aaataacgcc cgttttctgg cgttccggca cccctgccag cgtaggcagc
    12301 agggaaacgc cgtcacaacg ggacggcacg ggcactccgg ccacgtccgc aaaagtagcc
    12361 atccagtcct ggaactggct ggcattcaaa ctgattccgc tgggaaccac accgggccag
    12421 cgtaccacgg ccggaacacg catgccgcct tcccagcaat cgcgcttaat gccgtccatc
    12481 atcccgtagc tcatgaaata cgcgggatcc tgcgcgccgt tgccgtaatt gccggaaccg
    12541 ggttcattgt gggggccgtt gtcggacgtg aagacaatca tcgtattgtc atcaatgcct
    12601 aaatccttaa gcagctggat catgtccttc aggacgtcat ccacccggcg aatcatcgtg
    12661 gcgtggcggg attccgttct tccaaaaacc tcatgttccg ggtagatata ggtatccgga
    12721 gaaaaatccc cctccacccc cgaccatccg tccgcagcgg tattgcaggc ttcatagccg
    12781 tcttttttct cccactggag gccgccgtcc cttcccagcc ccgccggata ggcacaggcg
    12841 ggagcggaca gactgccgtg gggagccggg aaagccagat acaggaagaa aggccgggaa
    12901 ggagttttct catggtggtc cacaatccac tgtttggcgc gggccccgaa caaatccgta
    12961 tcataggcgg tgcccggaac tttcgcgctg acgctctccc acgccggagc cgcggcctgg
    13021 tcattatatt cgtaaatatc ctgggaaccc gccgtcagat aatggtaatg ccccgccaaa
    13081 tgcttgatga ttccgtaaaa ataatcaaac ccgcgcatgt ggggagccgc ggtcagagcc
    13141 ttgccgctct gtccgcctcc gcccacgccc catttcccga tggccgccgt atggtaaccc
    13201 gcgcttttca gcagcgttcc cagcgtatgg gaattctcga tagggtaatc aaacgtattg
    13261 ttgcgcacca cccgggaatg cccctggtga acgcccagca gcagggaagc gcgggccgga
    13321 gcgcataccg gagccgcact gtaatggcgc ctcagctgca tgccctccgc cgccatcgtg
    13381 tccagcgtag gcgtcttgaa ttcattcctg cgggtcacct gcctgccgtt tttatcctgc
    13441 tgcgtccagt tgacgctcat atccccccag cccatgtcat ccaccaggac aaaaataatg
    13501 ttgggcctgg ctccggaaac atgcgccggc atgtccggct tttccgctac atccgtctgc
    13561 agcgtcagcc agccggatgc ggcatcataa ttccatgaaa ccgccccttc aggcagttcc
    13621 tttacggtga cggggacgcc catatccggc cggaactgca cgcccagcgt atgcctgccc
    13681 ggccattcgg aaatcaaatc ctcaaaggag gaatcaaaca aaaccacata ttcccttgcc
    13741 tgctgggcag gggtttccgg ccggacggcg gagttttgcg gcacgccgtc cagcgtagcc
    13801 acatggagac gccccagcgt cagggatgaa ctgggcaggg aaatatcccc atccacagtc
    13861 agggtgcctc ccggcgcata agtaatcgtt cctgaaaagg cgccgctgcc gcccagcgcg
    13921 cagctgccct gcaccaccag gtccaccgcc ggtatttctc cgtcaaaatg catggaggag
    13981 ttattcagcg ttatcgtcac attgctgcct acgctccgga tttcccccgc cttgacattc
    14041 ctgtccgtat aagtgacccg ggcatcatcc agaagcgtct gccgcagcgt tcccttatcc
    14101 ccggtaatgc cggaagagcc ggaaagaatc acgttgccgt ccacacccgc ctccgtcagg
    14161 tccagcgtgg aattttccag cgccaactcc gtgtccgcgc caatgttcca ttgtccggac
    14221 gccttggcct ccgttccgga aaaagtcacg gacgcgtttt cccgcgtcac cagggccccc
    14281 tggtacaaag acatgccttc caggcgggca gcgtcttcca ccgtcaccgt attgcccatg
    14341 ccccggtccg ccagatccag cgtcggaatt ccctttccgg cgttcagcac aaaaccgatg
    14401 aggccggaaa tattgttcag cagcatccct tccccgatgc gcacggcgcc tccgcccgtc
    14461 actgtcacgc gccgcggaac atcgccttcc gaagtcaggg aatacaatcc cccggacgga
    14521 gcgtcatcgt catgccccac atacaggctg cgcccttcag ccacatgcca ctcctggtcc
    14581 gcggctacgc gcagctgggc gaccccgaaa tccgcagaat aaccgccttc tccggcattt
    14641 tccacggtaa tgccgccctc ctccacgcgc aaatatacat ccttgttcgt gtttttaatg
    14701 aacaatggga ttttgccttc cggctggctg ttaagcccct caatcctgac gcccttcaaa
    14761 tagcggcccc ccgcgcccaa tccgccgaca tccgtccttc ccgcggaatc cgttccattg
    14821 gttaccatcc aatgttcata ggcggaagcg gcggcccctg gcgcggaaga ggcggcaacg
    14881 cctccgacac tccagttgga cagggaaccc cactggtaat cggcacccct ccagacatat
    14941 tcaacatcgg catatccggc tggagcaagg aacagaagaa aaaagaggag aacggtaaaa
    15001 aaacggtgcg ggaacatggc tggctggcga aacgatgacc cggaggacat cacgccgaag
    15061 aacggactgc ctggggaatc ataccggtta ttgatattaa atgttaaaaa atgccgaatt
    15121 ttgttggctg aaaatgaaca agccgccagt ttcaaagaaa aaacggacgg cttgaacaag
    15181 tgtatcaggg acatgggctt caacgtctgc ggcgacgcat catcaatgcg gccagcccca
    15241 aaagccccaa agaagccgta gcaggttcgg gaaccacttc ccccttaatc tgaattcccg
    15301 taagaccgat aaaacagccg ctgccgtagg cctcgttttt ggaagccgta acagttaacg
    15361 tcatggattc cccggctttc aacaacacat cgccatattt atagcttccc gtatgaacgc
    15421 catcccagga tcccgtagat gtatttgtac tggtggcgcc attataggcc tgcatctgat
    15481 tgtcctgccc gctcatgcca agggtgatat tgacaggttt gttcagattg ccgtcggctc
    15541 ccccaacata attgttattg gccacaccgc ctccggcatt ctgcgcggcg cccgctccag
    15601 tgaaccctat catggaaaga tcaatggaag aaatcagcat atcctgggtg ccgccgttgg
    15661 taaacgtgaa agaaacgcgc catgtattat tctgattatt acccacattg gcattaggag
    15721 aaaatacgga ggccggaata gtgccgttag ccagcaggtc attgcccgaa atatttttgc
    15781 tgaaattatc ggagctaatc gaggtggaag tggagagtcc cgcatcggag tccgtactca
    15841 cagtgacgga atccagggaa gtcccggtcc ggttaaacgt agtggtgcac agcaccgcgg
    15901 cctgggaagc cgccgcgcca agagccaggg acgcaatgat tatcaatgtt ttcttcataa
    15961 ctggggaagg ttgaatggtg tcaacctttt ccgtttagcg gattacaaat ccgaaaagta
    16021 aatccactcc tttttattat gcataaataa ttggtgttgt aaattttatg atagtttcaa
    16081 atctcccgca gggaagcttc tgattatagg acgcctctgt tcctatattt gtataaacat
    16141 c
//
LOCUS       DAOMSU010000021        15881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_134_length_15881_cov_3.079300, whole genome shotgun sequence.
ACCESSION   DAOMSU010000021 DAOMSU010000000
VERSION     DAOMSU010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15881
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_134_length_15881_cov_3.079300"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2296)
                     /locus_tag="PK077_01860"
     CDS             complement(<1..2296)
                     /locus_tag="PK077_01860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRN23075.1"
                     /translation="MVPQKENVVNCPACGQPMDVSLLPPYANAICPGCQALTRVKTRM
                     GPYRITGKLGKGGMSVVFRAEDAVLGREVALKVLNDTYAGDALRCERFEREARIMARV
                     SHENLVQLYAVGRDQGLFYIAMELVEGNGLDALIAAEERVPEDRVLRLTLDIVRGLDA
                     AWNAGLMHRDIKPANVLLSPDGVVKIVDFGLSLLHSESDMEREIWVTPYYAAPETLLR
                     GEEDFRTDMYALGATMYHLLAGAPPRVDASQSSDILLENKKNLPLLEQVVNDISPMTC
                     FIVDRLMAFNKEDRFSSYPELMDAVEQAQEEYARAMQESGLTWAERRAVEARHARRRK
                     CRIIVASSIVSVVALALGVWGWAEWREDGAPSPVTPPSPISPAAEAALEDREAAESRL
                     ERSIRFGKLFNEAQESLNRGDLVKASAMFGDLAAQPDCPLSTSLWAGLNQVLCMWVRG
                     QFPEGVKRLEELGRKMEACGDPEELERSRDIGNLVMYLSSADWSSRMPGLRSNSDLAV
                     HYYVGMALKSWYFAGRWTEYGGFLDRVAADAADDRDRNVRELASAWLSNLEKYTDQYA
                     RLKRLQDMPEKTVAEVEAKRSAADFLREQMVIGEVPSMSPAYAALEGILDHLRMQYQA
                     ARDWEATEAVKASERKKAEEMQKRREILLAAQSRSYEDVCREAEGIMKETGDYEKVST
                     VYGEAEKVISSPAVKARLAMRREMTDQMLPLFSRMEKLLPVLLEKKPGKTLVLRDGSR
                     VRLKGMKGHLLTVEPQDSREGSGAY"
     gene            complement(2428..3837)
                     /locus_tag="PK077_01865"
     CDS             complement(2428..3837)
                     /locus_tag="PK077_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007415563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRN23076.1"
                     /translation="MLRSQRIIAMNIQPKITPVLDPGFVPAVLWNQAFEAKAAADPAS
                     HQVDIALTRNDGTCFRWSGKLLPHTGENIALNETYVERIVKFLLWQKGGNIILVAGDD
                     AIADMLASRYCKGGIREFDWDFIGKKIYGSPIEVKKVSVEELPEEYSGSMTLGRNLDG
                     CRIGFDLGGSDRKCAAVVNGEVVYSEEVVWDPYFQKDPQYHIDGIQDSLERAAAHLPR
                     VDAIGGSSAGVIINSEVRTSSLFRGVSQEDIEKTLGKVFRTLQKEKWNNIPFEVVNDG
                     EVTALAGAMGMNDNAVLGVAMGTSEAAGYVDPEGHIKPWLNELAFAPVDYSEEGGVDE
                     WSKDMGVGALYFSQQAVARLAPRAGFQFEGMPFPEQLKKVQAAMAEGDERARKIYETI
                     GVHFGYAIAHYARFYDIRNLLFLGRVASGDGGQIIIDKAEEVLRTEFPELKIQLRVPD
                     EKTKRHGQAVAAASLPALS"
     gene            4011..4874
                     /locus_tag="PK077_01870"
     CDS             4011..4874
                     /locus_tag="PK077_01870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRN23077.1"
                     /translation="MNELTDQLITLFSSILITVMFLAVAVNIFRRNHAPDTRHPLKWR
                     IPVGHLDMLDIAMCGIIVLYFSLGALLAAATPPGASAPPSTTNMELDSYKVFNGTCLN
                     LILAFVLLVRMFHTGRMEALGLKKHSLKVLLYAPAAGYLLVLAIHFLLDKAGLFQWIK
                     HATNAPAEQSIVSVLRHSSDIPLITVICFSAAIAAPLVEELIFRGYLYPIMKKYTGVW
                     FALITTSLLFGIIHVSLVPFIPLAIFGAVLVLLYEYTGSIWTPIIAHCIFNTATLINI
                     LYPGILLPYGT"
     gene            4864..5760
                     /locus_tag="PK077_01875"
     CDS             4864..5760
                     /locus_tag="PK077_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HRN23078.1"
                     /translation="MEPEPSIRQTGEDALVARLLPLMPSNPSLETGPGDDCAIIRGSG
                     NRQLLLKTDCVVEGMHFLPGTDPELIGRKALARAVSDIGAMGGTPLHALVTLFVHADR
                     PISQVEGIYRGMGRLARQFGISIAGGESSGLPADGLIINVALTGEVEEGKAVLRSTAR
                     AGDLIAVTGTLGGSFPTGHHLSFMPRVREGGILAASGVATSMMDLSDGLGTDLPRLAA
                     ASGLGFRIHEELLPVRPGFTAAQAVGDGEDYELLVTFRPGDRERAAQLAAEHFPKTPL
                     TVIGEMTAETASALPPGYRHFS"
     gene            complement(5805..7646)
                     /locus_tag="PK077_01880"
     CDS             complement(5805..7646)
                     /locus_tag="PK077_01880"
                     /inference="COORDINATES: protein motif:HMM:NF039989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRN23079.1"
                     /translation="MAQLKHPKMVDIRDILDENTRLPALVAASAEKLLGLERLNKAYD
                     KIVRDKESGSQENFFQLASRHLNLKLQLRPGDLENIPKKGPVVVVANHPHGLSDGIMF
                     GELLTRVREDVRILVNEQLSLCGELDPWLIKVDVYEDENARRKNLSGMRKMISWLRQG
                     GVLGIFPAGTVSSFSLAHKRVTDDPWNANIAAIIRMTKATVVPVYFPGRNSLLFQGIS
                     LINRKARVAFLPREVGRDGRRTHRIVVGKPIPFSQLGQYDSDEAIVSHLRLRTYLLGK
                     SYEKSRRPHVHKKDRKAKMAALTSPVSVQDMQAEIDALPPECLHARQENGDWDVYVAD
                     ALQIPNILIEIGRLREYTFRQVGEGSGNACDLDTYDNHYKHLFLWDRTHQKIVGAYRM
                     GETDKILARYGVKGLYNGEYFSFSPAALRVLDRSLEMGRAFIVPEYQKRPLALGFIWE
                     GIGQFMARNHHYRYLFGTVSISRDYTNLSRALIVSYLKAHEMEPVLVNEVRAYNPPRK
                     ADLKRSESCILPLGLADAQGLSQLVADIEEDGKGIPVLLRQYLKLNGKILSFSVDKHF
                     GDVLDCLILVDIFKTPERSIKRYLGKDAYEQLLPYMQREKEEEKAAE"
     gene            7785..8426
                     /gene="cmk"
                     /locus_tag="PK077_01885"
     CDS             7785..8426
                     /gene="cmk"
                     /locus_tag="PK077_01885"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRN23080.1"
                     /translation="MHPAIAIDGPAASGKSTVAKLIADRLGYTFINTGAMYRAVTWYM
                     LEQGINPADTAAVLESLPAVPLSFGKDGSQSVVLCGQHVLGEELTSQQVNDHVSTIAA
                     IPEVRALLVERQREYNRREPVVMEGRDIGTVVFPDTPFKYFVTASEEVRAARRAAEGL
                     TDSIAERDRKDSSRATAPLAQAPDALLVDTSDMTIDQVVRFITDNIQQKLSSR"
     gene            8423..9034
                     /locus_tag="PK077_01890"
     CDS             8423..9034
                     /locus_tag="PK077_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRN23081.1"
                     /translation="MNSFYWVFYTLFKSISKAFFSWKVVNREKLIEDGPVLIVSNHQS
                     FLDPPMLGISYEDGIYFFARKTLFQGIFKWALPLCQAIPIDQENPDAASLKHVIRLLK
                     SGKRVLVFPEGSRTPDGKIHDGMGGIGLILSKTKVPVQPLRISGAYEAFPIGARFPRL
                     RPVTVTVGDPIPFTPAELNAKGKDAYQHLTDKIMDAIRSLPAE"
     gene            9052..10107
                     /gene="recF"
                     /locus_tag="PK077_01895"
     CDS             9052..10107
                     /gene="recF"
                     /locus_tag="PK077_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRN23082.1"
                     /translation="MISRLKLMDFRCYGSFSWQIPQQGAIILGNNARGKTSLLEAVCF
                     LLRLQSPRTARTGPLVSHGKQSFGIRGELPGQIRRILWAPDAPDLRVNGEPRKDQRSY
                     LADSYPVVWMGNDDLSLVQAGADARRKYMDFLGSQWHPGYRLALFSYRRALKTRNYLL
                     KHRHRDKLQLDAYTRQLALHGTELRNLRAHLLALLAPHIALAYRNIGGRQEQVSIAYR
                     ASEEGDLYERLCASMDRDIRYGQTQNGPHRDDLDITLNGRNAAQFASEGQQRTTAISM
                     KLAQSSLLTEETGHTPIHLIDDVFGELDPTRRIAFLQSLPADAQSLITTTHLDWLHDA
                     PCPLPAFRLEDGTLAPL"
     gene            complement(10162..10668)
                     /locus_tag="PK077_01900"
     CDS             complement(10162..10668)
                     /locus_tag="PK077_01900"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRN23083.1"
                     /translation="MMKKIEPKEIQDNAVQLIGDDWMLVTAGSPEHFNMMTASWGGLG
                     FMWKKPVAFVVIRPQRHTFRFIEAGDELTLSFFSHEYHKALTVCGTTSGRDTDKVAAS
                     GLTPYVTENGNVSFTEARLVLECRKLYAEPLNPEAFLDKSIVPEWYAAGDFHKMYIVE
                     ILNVWVKE"
     gene            10829..11524
                     /gene="rnc"
                     /locus_tag="PK077_01905"
     CDS             10829..11524
                     /gene="rnc"
                     /locus_tag="PK077_01905"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRN23084.1"
                     /translation="MNHTALEDKLGYRFNNPDLLVQALTHPSTDSKPETRRAYERLEF
                     LGDAILQLAVTQYLYHHMPQSPEGELTQLRARTVSRANLGKYGFILGLDKHIALGKGE
                     ERAGGRSKNSIIANTFESVFGAMSLDSNYETAKAVALRVLHEALDSAASHPKEINPKG
                     ELQAILQDILPETPSYETEEKGPRDAENRFESRVFWHGHAIGAGSGASKRKAEVAAAA
                     DALDARAWLRMTL"
     gene            11668..12999
                     /locus_tag="PK077_01910"
     CDS             11668..12999
                     /locus_tag="PK077_01910"
                     /inference="COORDINATES: protein motif:HMM:NF019042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HRN23085.1"
                     /translation="MKTYTLPVIGLMTLGASAFAAAAPSTGNSLPARDNFSACKWLGD
                     RFKWFNSERDHKNPYIQEFNVSLRMQYGLDWIDPNGEGRVMGEKEGNGRRFNDEWRRF
                     RAGFNMKFLNNFKLNNVWNIGGMDGLESYNAKTNTWDTSDLTYSLYELNLEYKGGPVT
                     YAIGKIKPRITGEYRTSSSAILTIERSMLVNQLRSETNYGFQVNNSDKKDKLGWAAGV
                     WLNGNGGTGTGTFNNRIEPAFNSRDNCFITGTLSYDTSNDVFLKKSRLWLDYAHNFTK
                     WGDDAQNKAYEKLTGYGFKSKYQGTGARDVVALTWEGSEGDFSLMTEVMAGFNVIGMK
                     AGAENVFGVTVMPSYKFTPHWEGVVRYQMAAGSNAVKWEKRYTQNSTYSGTSDCMQAL
                     YLGVNYYIFECSPNMAKIMAGIEYAHSNGTDASRQKGFTGWSYNIAFRTNF"
     gene            complement(13042..14109)
                     /locus_tag="PK077_01915"
     CDS             complement(13042..14109)
                     /locus_tag="PK077_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRN23086.1"
                     /translation="MKEEISETDHAALAILKKTGLDVVEAAQLAHELLQASKGRGSRW
                     KRARECIRLGEEALVARGKTVVFRKAVDEAMEARQDRRKRTRDDFRYISRRLLRFCPE
                     IARRPVRFITPRECRACLEKSFDTLRQRHKARLVLSGIFGTAVKRGWCTENPVAHVDL
                     PRLKEHSIPVLSLEEMRRLLAAAEEYDGGACLAAVGLMLYAGIRPEEVRRLDWAQINL
                     REGMVSLRARHSKTGGARIVTIQPVLGNLLERAVAMGSVSGSVCPRNWPAKWRELRRL
                     AGWDGKEKKWPADMLRHTFASYFARHFKNLHVLQMEMGHASSDLLRTRYLNMEGITEM
                     TAAVFWGNCHAGRHAIKNGRP"
     gene            14483..14845
                     /locus_tag="PK077_01920"
     CDS             14483..14845
                     /locus_tag="PK077_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23087.1"
                     /translation="MMKKTILAACAVLLALTTISCQKSDVDLCLNAQTELVETLQKIN
                     DRDSADKYADEVDKLMNQFFNLFNTINNTPEEEKLKDNPQWRPFWEQMGKLSKRLRDQ
                     GYYGSEKLRNAFSKGRGK"
     gene            14877..15239
                     /locus_tag="PK077_01925"
     CDS             14877..15239
                     /locus_tag="PK077_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23088.1"
                     /translation="MIKKTISVAGAVLLALASASCQKNNTEQYLDMQEEMIELMESIK
                     DRDSADKYAGKLADLRKKSEELEKSMPDEEKKKLWNKEEYGKILFRKEPLKQTLFKKD
                     YYGSYGLRKALSDNVFQP"
     gene            15279..15866
                     /locus_tag="PK077_01930"
     CDS             15279..15866
                     /locus_tag="PK077_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23089.1"
                     /translation="MFFIHALPAALLALAAASCSPIVAEYTYRGSVSGTSSRTGARML
                     TLKEYRDGTVSGKLSIPSSSAMEPYSCGSIGGRADDRRITFYLTSLGDSYLSAKMAVA
                     GNSAGTTIAYRGHISGNRRYLSGKWHAANDYRSYLRGGSFSFNLTHINGRAVTRKTGI
                     KKNGTESPPTEKGEIPTFSVESEGAGSTPTGFTAE"
BASE COUNT         3551 a         4972 c         4133 g         3225 t
ORIGIN      
        1 ggtacgcacc gcttccttcc cggctgtcct gaggctccac ggtcagcaga tgtcccttca
       61 tccctttcag gcgcaccctg gagccatctc tgagcaccag agttttcccc ggtttctttt
      121 ccaacagtac gggcaaaagt ttctccatgc gggagaacaa cggcagcatc tggtccgtca
      181 tttcccggcg catggccaga cgggctttca ccgcaggaga ggaaatgact ttttcagcct
      241 ctccataaac agtggaaact ttctcataat caccggtttc cttcatgatg ccctccgctt
      301 cccggcagac gtcttcataa cttctggatt gggcggccag caggatttcc ctcctctttt
      361 gcatctcttc cgcctttttc ctttcagacg ccttcacggc ttccgtagcc tcccagtccc
      421 gcgcagcctg gtactgcatc ctcaaatgat ccagtattcc ttccagcgcg gcataagccg
      481 gagacatgga aggaacttcc ccaatgacca tctgttcccg caggaagtcc gccgcggacc
      541 gtttggcctc tacttcagcc accgtttttt ccggcatgtc ctgaagacgt ttcaagcgcg
      601 cgtactggtc cgtatacttc tccaggttgc tgagccacgc ggaagccagc tcacggacgt
      661 tcctgtcccg gtcatccgcc gcatcagcgg ccacccggtc cagaaagcca ccgtattccg
      721 tccatctccc ggcaaagtac caggatttca gcgccatgcc cacataataa tgcacggcca
      781 ggtcagaatt ggagcgcagc cccggcatcc gggaagacca gtctgcggag ctcaggtaca
      841 tgaccaggtt gccaatatcc ctggaacgtt ccagttcctc agggtccccg caggcttcca
      901 tctttctgcc cagttcttcc agacgcttca ccccttccgg gaactggccg cgcacccaca
      961 tgcacagaac ctggttcaaa ccggcccaaa gggatgtaga cagagggcag tccggctgag
     1021 ccgccaaatc gccgaacatg gcggaggctt tcaccagatc cccacggttc agggattcct
     1081 gggcttcgtt aaacagtttg ccgaagcgta tgctgcgctc cagccgcgat tccgccgcct
     1141 ccctgtcttc caaggctgct tccgccgcag gagaaatagg cgaaggcgga gttacgggag
     1201 aaggagctcc atcctcccgc cattccgccc atccccaaac gcccaacgcc agcgccacta
     1261 cggaaacaat ggaggaggca acgatgatgc ggcatttcct gcgccgtgcg tgccgggcct
     1321 ccaccgcgcg ccgttccgcc caggtgagac cggattcctg catggcccgg gcgtattcct
     1381 cctgcgcctg ttccacggca tccatcaatt cgggatagga agaaaacctg tcttctttat
     1441 taaaagccat cagtctgtcc acaatgaaac aggtcattgg agaaatgtcg ttcaccacct
     1501 gctccagaag agggagattt ttcttgttct ccagaagaat atcggaagac tgggaagcgt
     1561 ccacacgggg aggcgcacct gccagcaaat ggtacatggt agcacccaaa gcgtacatgt
     1621 ccgtacggaa gtcctcctct cctcgcagca gcgtttccgg cgcggcgtaa taaggagtaa
     1681 cccatatttc ccgctccatg tcggattcgc tatgaagcag ggacaggccg aaatccacga
     1741 ttttgacgac tccgtcgggg gacaaaagaa cattggcggg cttgatatcc ctgtgcataa
     1801 ggcccgcatt ccatgcggcg tccagcccgc gcacgatatc cagcgtcagg cgcagcaccc
     1861 tgtcttccgg cacacgttcc tccgcagcaa tcagggcatc caggccattg ccctccacca
     1921 gctccatggc aatgtaaaaa aggccctggt cacgcccaac ggcataaagc tgcaccagat
     1981 tttcatggga aacccgcgcc atgatccggg cctcccgttc aaaacgttcg cagcgcaggg
     2041 catccccggc ataagtatca ttcagcactt tcagggccac ttcccgcccc agaacggcat
     2101 cctccgcccg gaacaccacg ctcatgccgc cttttcccag ttttccggta atacgataag
     2161 gccccatgcg cgtttttaca cgcgtcagcg cctggcagcc cgggcaaatg gcgttggcgt
     2221 acggaggcag cagggaaaca tccatgggct gtccgcacgc cgggcaattg acgacgtttt
     2281 ctttctgggg aaccatgaac gcagggcggc ggaacgggaa tcagaaaaaa ggaaggagga
     2341 aaaatggcgc ggattgtccg cccccctccg aatatgagca gggagggacc ccagccaggg
     2401 cccctcccgc aaatttgatt aatcccgtca ggagagagcc ggaagggaag cggcggctac
     2461 ggcctgtccg tggcgcttgg tcttttcatc cggcacgcgg agctggatct tcagctcggg
     2521 gaattcggtg cgcagcactt cttccgcctt gtcaatgatg atctggccgc cgtctccgga
     2581 agccacacgg cccaggaaga gcaggttgcg gatatcatag aacctggcgt aatgggcaat
     2641 ggcatagccg aagtgcacgc cgatggtttc ataaatcttg cgggcgcgtt cgtcgccttc
     2701 cgccatggcg gcctgaacct ttttaagctg ttccgggaag ggcattcctt caaactggaa
     2761 gccggcgcgg ggggccaggc gggccacagc ctgctgagag aaataaaggg cgcctacgcc
     2821 catatccttg gaccattcgt ccactccgcc ttcctccgag tagtccacag gggcaaaagc
     2881 cagttcattc agccagggct tgatgtggcc ttccggatcc acgtagccgg cggcctcgga
     2941 ggtgcccatg gccacgccga gcacggcgtt atcgttcatg cccatggccc ctgcgagggc
     3001 ggtgacttca ccgtcattca ccacttcaaa gggaatgttg ttccattttt ccttctggag
     3061 ggtgcggaat accttgccga gagtcttttc aatgtcttcc tggctgacgc cgcggaacag
     3121 ggaggaggtg cgtacttcac tgttgatgat cacgccggcg gaggatccgc cgatggcgtc
     3181 cacccggggg agatgagcgg cggcgcgttc cagagaatcc tgaattccgt caatgtgata
     3241 ctggggatcc ttctggaagt aagggtccca gaccacttcc tcggaataaa ctacttcccc
     3301 attcaccacg gcggcgcatt tgcggtcgga accgcccagg tcaaaaccga tgcggcatcc
     3361 gtccaggttg cggcccagtg tcatggagcc ggagtattcc tcgggaagtt cttccacgga
     3421 aacctttttc acttcaatcg gggagccgta aatcttcttg ccgatgaaat cccagtcgaa
     3481 ttcgcggata ccgcccttgc aatagcggga ggccagcatg tccgcaatag cgtcatcccc
     3541 ggcgacgagg atgatattgc cgcccttctg ccacagcagg aatttcacaa tgcgctccac
     3601 ataggtttca ttcagggcaa tgttttctcc ggtatgggga agaagcttgc cggaccagcg
     3661 gaaacaggtc ccgtcattgc gggtcagagc gatatccact tgatgagaag caggatcggc
     3721 ggcggctttg gcctcaaaag cttgattcca aagaacggcc ggcacgaagc cggggtccag
     3781 cacaggcgtg attttgggtt gaatattcat ggcgataatc cgttgggaac gtagcatacc
     3841 cgcttttcct tgaaaagtat tcattccctt tccttatgat tttttttgcg gaatgagggg
     3901 agatagctca ttttctgctg gaaaatcacc gtgtttccac taaagagaca ggagcttttc
     3961 ttccggcgcg gccccctgga agggcggccc attccacaca tgcctattcc atgaatgaat
     4021 taacggacca gctcattacc ctgttctctt ccatcctgat taccgtcatg ttcctcgccg
     4081 tggcggtaaa catattccgc cggaatcatg cgcctgatac ccggcatccg ctgaaatggc
     4141 gcattcccgt aggccatctg gacatgctgg acatcgccat gtgcggcatc atcgtgcttt
     4201 acttcagcct gggggccctg ttggcggcag ccacgcctcc gggggcttcc gctccgccca
     4261 gcacaaccaa catggagctg gacagctaca aggttttcaa cggaacctgc ctgaatctca
     4321 ttctggcctt cgtcctgctg gtgcgcatgt tccatacggg ccggatggag gcactgggat
     4381 tgaaaaaaca ttccctgaaa gtcctcctct acgctccggc ggcaggttat ctgcttgtgc
     4441 tggccatcca tttcctgctg gacaaggcgg gcctgttcca gtggattaaa cacgccacca
     4501 acgccccggc cgaacaatcc attgtttccg tgctgaggca ttccagcgac attcccctga
     4561 tcacggttat ctgcttcagc gccgccatcg ccgcccctct ggtggaggaa cttattttcc
     4621 gcggttatct gtacccgatc atgaaaaaat acacgggcgt ctggtttgcc ctgatcacca
     4681 cctccctcct cttcggcatc atccacgtca gcctggttcc gttcatcccc ctggcaatct
     4741 tcggagccgt actggtgctg ctgtacgaat acacgggcag catctggacg cccatcatcg
     4801 cccattgcat tttcaacacc gccactctca tcaacattct gtatcccggc atccttcttc
     4861 cgtatggaac ctgagcccag catccggcag acgggagagg atgcgctggt agcccgcctc
     4921 ctgcccctga tgccttccaa tccgtccctg gaaacggggc cgggagacga ctgcgccatc
     4981 atccgggggt caggaaaccg ccagttgctc ctgaaaacgg actgcgtggt ggaaggcatg
     5041 catttcctgc ccggaacgga cccggagcta atcgggagga aggcgctggc acgggccgtt
     5101 tcagacatag gggccatggg cggcacgccc cttcacgcac tggtgaccct gttcgtgcac
     5161 gcagaccgcc ctatctccca ggtggaagga atttaccgcg gcatggggcg gctggcgcgg
     5221 cagttcggca tcagcatcgc cgggggggaa agctccggcc tgcccgcgga cgggcttatc
     5281 atcaacgtgg cgctgaccgg agaagtggag gaaggcaaag ccgtactgcg cagcacggcg
     5341 cgggcggggg atctgattgc cgtaacggga acgctcggcg gcagctttcc caccggtcac
     5401 catctttcct tcatgccgcg tgtcagggaa ggaggcatcc tggccgcctc cggcgtagcc
     5461 acctccatga tggatctttc cgacgggctg ggcaccgacc tcccccggct ggcggccgcc
     5521 tccgggcttg ggttccgtat tcatgaagag cttcttcccg tgcgcccggg gttcactgcg
     5581 gcccaggctg taggggatgg ggaggattac gaattgctgg tgaccttccg tcccggcgac
     5641 cgagaacggg cggcacaact ggccgcggag cattttccga aaacgcctct caccgtcatc
     5701 ggggaaatga ctgcggaaac ggcctccgcc cttcccccag gttacaggca cttcagctag
     5761 cccgttaaga atcgggggca gagccacgca aaacgcctgc ggaattattc cgccgccttt
     5821 tcctcctctt tctcccgctg catgtaaggc aggagctgtt catacgcgtc tttgcccaga
     5881 taacgcttga tggaacgctc cggcgttttg aaaatgtcca ccaggatcag gcagtccaat
     5941 acgtcgccaa aatgcttgtc cacgctgaag gaaagaattt tgccgttcaa tttcagatat
     6001 tggcgaagca atacgggaat gcccttgcca tcctcttcta tatcagcgac caattgggac
     6061 agcccctggg catctgccag ccccagaggg aggatgcatg attccgacct cttcagatcc
     6121 gctttacggg gcggattgta ggccctcacc tcattcacca gcacgggctc catttcatgc
     6181 gccttcagat aggagacgat gagggcgcgg gaaaggttgg tgtaatcccg ggaaatgctg
     6241 accgtgccga acaggtagcg gtaatggtgg ttgcgggcca tgaactggcc gatgccctcc
     6301 caaatgaaac cgagagccag cggcctcttc tggtattccg gaacaatgaa ggcgcgcccc
     6361 atttccagag agcggtccag cacccgcaaa gcggcagggg aaaaggaaaa gtattccccg
     6421 ttgtacagcc ctttgacacc gtagcgggca aggattttgt ccgtttcccc catgcggtag
     6481 gcgcccacaa ttttctggtg cgttctgtcc cacaggaaca ggtgcttgta atggttgtcg
     6541 tatgtatcca ggtcgcaggc gttgccggaa ccttccccta cctggcggaa agtatattcc
     6601 cgcaaacgcc ctatttcaat aaggatattg gggatttgca gggcatccgc cacgtacacg
     6661 tcccaatctc cattttcctg acgggcatgc agacactccg ggggcagcgc gtcgatttcc
     6721 gcctgcatat cctgcacgga aacgggggag gtcagcgcgg ccatcttcgc cttccggtcc
     6781 tttttgtgca catgggggcg gcggcttttt tcataacttt tacccagcag ataggtgcgc
     6841 aggcgcagat gggagacgat ggcttcatcg gaatcatact gtccaagctg gctgaacgga
     6901 atgggcttgc ctaccacaat gcggtgtgtc cggcggccgt cccgacccac ttcacgggga
     6961 aggaacgcca cgcgcgcctt gcggttgatc agggaaatgc cctggaacag caggctgttg
     7021 cgccccggga aatatacggg caccaccgtc gctttcgtca tgcggataat ggcggcgata
     7081 ttcgcgttcc aggggtcgtc cgtcaccctt ttgtgagcca aggaaaaact ggagaccgtg
     7141 ccggcgggga aaatgcccag aaccccgccc tgccgcagcc aggaaatcat cttgcgcatg
     7201 ccggaaaggt ttttgcgccg ggcgttctca tcctcataca cgtccacctt gatgagccag
     7261 ggatccagct ctccgcacag ggaaagctgc tcgttgacca ggatgcggac atcctctctt
     7321 acgcgggtaa gcagctcccc gaacatgatg ccgtccgaca agccgtgcgg atgattggca
     7381 acgacaacga caggcccttt cttaggaata ttttccaggt cccccgggcg cagctgaagc
     7441 ttcaggttca aatgcctgga ggcaagctga aagaaatttt cctgagaacc ggattctttg
     7501 tcgcgcacga tcttgtcgta cgccttgttc aggcgttcca ggcccagcag cttttcggcg
     7561 gacgcggcca ccagagcagg cagacgcgta ttctcgtcca aaatgtctct gatgtccacc
     7621 atcttcgggt gtttgagctg ggccatgccc cgctgtataa cggatttacg ggaaaaagtg
     7681 aaactctttt tgcaacgccc ttcggaacgc agtcttgatt tccacagggc tcaaagctac
     7741 aattcaaacg gctttagccc ccatttccaa ctcagaagac actcatgcat ccagccatcg
     7801 ccattgacgg ccccgccgcc tccggaaaat ccaccgtcgc caagctcatc gcggaccgcc
     7861 tcggctatac gttcatcaac accggggcca tgtaccgggc cgttacctgg tacatgctgg
     7921 aacagggcat caaccctgcg gacacggcgg ccgtgctgga atccctgccg gccgtgcccc
     7981 tctctttcgg caaggacggc agccaatccg tagttctgtg cggacagcat gtgctgggcg
     8041 aagaactcac gagccagcag gtcaacgacc atgtttccac cattgcggcc atcccggaag
     8101 tacgcgccct gctggtggaa cggcaacggg aatataaccg ccgggaaccc gtcgtgatgg
     8161 aaggaaggga cataggcacc gtggtttttc cggatacgcc gttcaagtac ttcgtcaccg
     8221 cctccgagga agtgagggcc gcgcgccgcg ctgcggaggg actgacggac tccatcgcgg
     8281 agcgggaccg caaggattcc tcccgcgcca ccgccccgct agcgcaggcg cctgacgctc
     8341 ttctggtgga tacgtcagac atgacgattg accaggtcgt ccgtttcatc accgacaaca
     8401 tccaacaaaa actctcctcc agatgaactc tttctactgg gttttctaca cgctcttcaa
     8461 aagcatttcc aaagccttct tctcctggaa agtcgtcaac cgggaaaaac tcatcgaaga
     8521 cggccccgtg ctcatcgtca gcaatcacca gagctttctg gatccgccca tgctgggcat
     8581 ttcttatgag gacggcatct attttttcgc acgcaagacg ctgttccagg gcattttcaa
     8641 atgggccctg cccctctgcc aggctatccc cattgaccag gaaaacccgg atgccgccag
     8701 cctcaagcac gtgatacgcc tgctgaaatc cggcaagcgc gtactcgtgt ttccggaagg
     8761 ctcccgcacg ccggatggta aaatccatga cggcatgggc gggatcggcc tcatcctgag
     8821 caaaacgaaa gttcccgtgc agcccctgcg catcagcggt gcgtatgaag cctttcccat
     8881 cggcgcccgg tttcccaggc tccgccccgt cacggtcacg gtgggagacc ccatcccctt
     8941 cacgccggcg gaactcaacg ccaaaggaaa agatgcatac cagcacctga cggacaaaat
     9001 catggacgcc atccgctccc tccctgcgga ataacccctt ttcccggccc catgatctcc
     9061 aggctgaaat taatggactt ccgttgctac ggaagcttct cctggcagat tccgcagcag
     9121 ggggccatca tcctgggaaa caacgcccgg ggaaaaacca gcctgctgga ggccgtttgc
     9181 ttcctgctgc gcctgcaatc cccgcgcaca gcccggaccg ggcctctggt ctcccacgga
     9241 aaacaatcct tcggcatccg cggagaactt ccggggcaaa tccggcgcat cctgtgggcg
     9301 ccggacgctc cggacctccg cgtcaacgga gagccccgca aagaccagcg cagctacctg
     9361 gcggacagct atcccgtggt atggatgggg aatgacgacc tttccctggt gcaggccggc
     9421 gcggacgccc gccggaaata catggacttt ctgggcagcc agtggcatcc aggctacaga
     9481 ctggccctgt tcagctaccg ccgggcgctg aaaacgcgca attacctgct caagcacagg
     9541 caccgggaca aactccagct ggacgcctac acgcgccagc tggccctgca cggcacggaa
     9601 ctgagaaacc tgcgcgccca tctgctggcg ctcctggcgc cccatattgc cctggcctac
     9661 cgcaatatcg gcggaaggca ggaacaggtc tccatcgctt accgcgcctc ggaggaagga
     9721 gacctgtatg aacggctgtg cgcctccatg gaccgggaca tccggtacgg acaaacccag
     9781 aacggccccc accgggatga cctggacatc acgctgaacg gcagaaacgc cgctcaattc
     9841 gcttccgaag ggcagcagcg caccaccgcc atctccatga agctggcaca gtcctccctc
     9901 ctgacggaag aaacgggaca tacacccatc cacctgattg acgatgtctt cggggagctg
     9961 gatcccacgc gccggatagc ctttctgcag tcgcttccag cggacgcgca gagcctcatc
    10021 accaccaccc atctggactg gctgcacgat gctccgtgcc ccctgccagc cttccggctg
    10081 gaggatggaa cccttgcccc cctctgaaaa agcgcggaca gaaacaacga acaggcgtcc
    10141 gcctgcggca tttccgggac gctattcctt cacccatacg ttcaggattt ccacaatata
    10201 cattttatgg aaatccccgg cggcatacca ctccggcaca atagatttat ccagaaaggc
    10261 ttccgggtta agaggctccg cgtacagctt gcggcactcc aacaccaggc gcgcttccgt
    10321 aaaacttaca ttgccgttct ccgtcacgta tggcgtcagg ccggaggcgg ccaccttgtc
    10381 cgtatcccgt ccgctcgttg ttccgcacac ggtcagagcc ttatggtact catggctgaa
    10441 aaaggaaagc gtcagctcgt ccccggcctc aataaaacgg aaggtatggc gctgggggcg
    10501 gataacgaca aacgccacgg gcttcttcca catgaacccc aaaccgcccc aactggccgt
    10561 catcatgttg aaatgctccg gagaacccgc cgtcaccagc atccaatcgt ccccgatcaa
    10621 ttgtacggca ttatcctgaa tctcttttgg ttcaatcttc ttcatcatcg tctccaacgt
    10681 tcgttcgcaa actattctat acccctcctc ccatatgtca aagctgtgga gcggccggga
    10741 tgacgcggga aaacaaattc cgtccccgcc gggaagctct ctccgtttgg agttgcattt
    10801 ccggaacggc ggccctagca tttccgggat gaatcacacc gcgctggaag acaagctggg
    10861 ttaccgtttc aacaatccgg atctgctcgt tcaggccctt acccacccca gcacggacag
    10921 caagccggaa acgcgccggg cgtatgaacg gctggaattc ctgggagacg ccattcttca
    10981 gctggccgtc acacagtacc tttaccacca catgccccaa tccccggaag gggaactgac
    11041 ccagctgcgc gcccgaaccg tcagccgcgc caatctgggc aaatacggct ttatcctggg
    11101 gctggataaa cacattgccc tgggaaaggg ggaggaacgg gccggcggac ggagcaaaaa
    11161 ttccatcatc gccaacacat ttgaatccgt cttcggagcc atgtctctgg attccaacta
    11221 tgaaacggcc aaggctgtgg ccctgcgcgt cctccatgaa gctctggatt ccgcggccag
    11281 ccatcccaag gaaatcaacc ccaaggggga actccaggct atccttcagg acatcctgcc
    11341 cgaaactcct tcctatgaaa cggaagaaaa agggccgaga gacgccgaaa accgttttga
    11401 atcccgcgtt ttctggcatg gtcacgccat tggagccggt tccggggcca gcaagagaaa
    11461 ggcggaagtg gccgccgccg cagacgccct ggacgccagg gcatggctcc gcatgacctt
    11521 gtaaccgcca aagggggacg ggtggctatt ttgtcaccct taagccttgg acactcctgt
    11581 ggctggtttg atagacagaa gccgtctccg ggaggaagaa ccttccccgg aagaccttct
    11641 cacagacaaa ccaaccatag aaaacaaatg aagacctata ctctccccgt catcggcctc
    11701 atgactctcg gagcctccgc ctttgccgct gccgcaccct ctaccggaaa ctccctgccg
    11761 gcccgtgaca acttttccgc ctgcaaatgg ctcggcgacc gtttcaagtg gttcaactcg
    11821 gaacgggacc acaagaatcc ctacatccag gaattcaacg tttccctccg catgcagtac
    11881 ggactggact ggattgaccc gaacggcgaa ggccgcgtca tgggagaaaa ggaaggcaac
    11941 ggccgccgct tcaacgatga atggcgccgc ttccgcgccg ggttcaacat gaagtttctg
    12001 aacaacttca agctcaacaa cgtctggaac atcggcggca tggacggcct ggaaagctac
    12061 aatgcaaaaa ccaacacatg ggacacctcc gacctgacct actcccttta cgaactgaat
    12121 ctggaataca agggaggccc cgtcacctat gccatcggca aaataaagcc gcgcatcacc
    12181 ggggaatacc gaacatcctc ctccgccatt ctgactatcg aacgctccat gctggtcaac
    12241 cagctccgct cggaaaccaa ttacggattc caggtgaaca attccgacaa aaaggataag
    12301 ctcggctggg cagcaggcgt ctggctgaac ggcaacgggg gcaccggaac cggcacgttc
    12361 aacaaccgca ttgaacccgc tttcaattcc cgggacaact gcttcatcac cggcacgctg
    12421 agctatgaca cgtccaacga cgttttcctg aagaaaagcc gtctgtggct ggattacgcc
    12481 cacaatttca ccaagtgggg ggacgacgcc caaaacaagg cgtatgaaaa actgaccggt
    12541 tacggcttta aatccaaata ccagggtacc ggagccaggg acgtggtagc cctgacctgg
    12601 gaaggttccg agggggactt ctccctgatg accgaagtca tggccggctt caacgtcatc
    12661 ggcatgaaag cgggggctga aaacgtcttc ggcgtgaccg tcatgccgtc ctacaagttc
    12721 accccccact gggaaggagt ggtccgctac cagatggcgg cgggcagcaa cgccgtaaaa
    12781 tgggaaaagc gctatacgca gaactccact tattccggaa cttccgactg catgcaggcc
    12841 ctgtacctgg gcgtcaacta ctacattttc gaatgcagcc cgaacatggc caagatcatg
    12901 gcgggcatcg aatacgccca ttccaacggc acggacgcca gccggcagaa aggctttacc
    12961 ggatggagct acaatatcgc tttccgcacc aacttctaat tgccggacgg catcatcccg
    13021 tcaacagctg gccgcttctg ttcaggggcg gccattttta atggcgtggc gccctgcatg
    13081 gcagttcccc caaaaaacgg cagccgtcat ttccgtaatg ccttccatat tcaaatacct
    13141 ggttcttagc aaatcggaag aagcatgccc catttccatc tgcaatacat gcaaattctt
    13201 gaaatgcctg gcaaaataac tggcaaacgt atgccttaac atatccgcag gccacttctt
    13261 ttccttcccg tcccacccgg ccaaccgtct cagctccctc cacttcgccg gccagttgcg
    13321 cgggcatacg cttcccgaca cagatcccat agcaaccgct ctttccagca gatttcccaa
    13381 aaccggctgg atcgtcacaa tgcgggctcc tcccgtcttg ctgtggcgcg ccctcaggga
    13441 aaccattccc tcccttaaat taatctgtgc ccaatccagc cttctcactt cttccgggcg
    13501 gatgccggca tacagcatca gcccaaccgc ggccaggcac gcccctccgt catactcttc
    13561 cgcagcagcc agcaaccggc gcatttcttc cagggacagc acgggaatgg aatgctcctt
    13621 cagcctcggc aggtccacat gcgccacagg gttttccgtg caccatccgc gtttcacggc
    13681 cgttccgaaa ataccgctga gcaccaaccg ggccttatgc ctctgcctaa gggtatcaaa
    13741 tgatttttcc agacaggcgc ggcattcccg gggcgtgata aagcgtacgg gccttcgggc
    13801 tatttccgga caaaacctca acaaacgccg gctgatataa cgaaaatcat ccctagttct
    13861 tttcctgcga tcttgccgag cctccatagc ctcatccacg gctttcctga acactaccgt
    13921 ttttcctctg gcaaccaggg cttcctctcc cagccggatg cattccctcg ccctcttcca
    13981 tcggctgccg cgccccttgc tggcttgcag caattcatgc gccagctggg cggcttccac
    14041 cacatccaac cctgtctttt ttaaaatggc cagcgccgca tgatccgttt ctgaaatttc
    14101 ctctttcatt tcatctattt tgcaacggtt tcggaatccg tagtcaagta aaaatagtcg
    14161 tttattaatc aaaaggttcg gcaattccat tgatgatgtt tcaccgcacc gtcatatcag
    14221 cagttctctt ccaaacagtt tgatgacaaa gaggacgcag gaattcctat acggattccg
    14281 aattcgctat tcgcaaatgc aggtcttcta aacagaaact ggcgggcgct cagaacacgc
    14341 ttgaaatccg cagcgaacaa accagagcgt caatctcaac gtatcctaca gatcctcccc
    14401 ccaacccgca ataactatac cgtgccgggg catcctcatc ctcggagcgt catttcccct
    14461 aaccatcaac cattcattaa caatgatgaa aaaaacaata ctcgccgcct gtgccgttct
    14521 gctggcttta accaccatct cctgccagaa aagcgacgtg gatttatgcc tgaacgctca
    14581 aaccgaactg gtggagaccc tgcaaaaaat caatgaccgg gacagcgcgg acaaatacgc
    14641 tgacgaagtg gacaaactca tgaatcagtt tttcaatctg tttaatacaa taaacaatac
    14701 acctgaagaa gaaaaactga aggataaccc tcaatggcgt cccttctggg aacaaatggg
    14761 aaagctgagc aaacggcttc gcgatcaggg atattacggc tcggaaaaac tgcgcaacgc
    14821 tttctcaaaa ggaagaggca aataaacaca acgtcaactg tcaaccattc acaattatga
    14881 taaaaaaaac aatatctgtc gccggcgccg ttctgctggc cctggcctcc gcctcttgcc
    14941 agaagaacaa tacggaacag tatctggaca tgcaggaaga aatgatcgaa ctcatggaaa
    15001 gcatcaagga ccgggacagc gcggacaaat acgccggaaa gcttgccgat ctccggaaga
    15061 aatccgaaga actggagaaa tccatgcccg acgaagaaaa gaagaagctc tggaacaagg
    15121 aggaatacgg caaaatcctg ttccggaagg agcccctgaa acaaaccctg ttcaaaaagg
    15181 actattacgg atcttatggg ctgagaaaag ccttatcaga caacgtcttc cagccctgat
    15241 tttccatccc tccccctctg cattctgcat caatgaaaat gtttttcatc cacgccctgc
    15301 ctgcggcgct gctggcgctg gctgcggcct cctgctcccc catcgtggca gaatacactt
    15361 acagaggctc cgtctccggc acaagcagca gaaccggagc ccgcatgctg accctgaagg
    15421 aatacaggga cggcaccgtt tcaggcaaat tgagcatccc gtcatcttcc gccatggaac
    15481 cgtattcctg cggaagcatt ggcggcaggg ccgacgaccg gaggattacc ttttacctca
    15541 cctctctggg ggattcctac ctcagcgcga aaatggcggt tgcgggcaat tccgccggaa
    15601 ccactatcgc ctacagaggc catatctccg ggaaccgccg ttacctcagc ggcaaatggc
    15661 acgcggccaa tgattaccgc tcctacctcc gcgggggctc tttctctttc aacctgaccc
    15721 atatcaacgg cagagccgtt accagaaaaa cgggaatcaa gaaaaacggt acagaaagcc
    15781 cgcccacaga aaagggagaa attccaactt tcagcgtaga atccgagggc gctggcagca
    15841 cgcccaccgg ctttacggcg gagtagccct gcgaaacaat a
//
LOCUS       DAOMSU010000022        15019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_154_length_15019_cov_2.690524, whole genome shotgun sequence.
ACCESSION   DAOMSU010000022 DAOMSU010000000
VERSION     DAOMSU010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_154_length_15019_cov_2.690524"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="PK077_01935"
     CDS             complement(<1..809)
                     /locus_tag="PK077_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRN23090.1"
                     /translation="MIRQILPVVVAACVAPCFAAEGWITDMDAAKKQAAEQKKDLMIE
                     FTGSDWCPPCMQLRANVFSKPDFQKEAQKNFVLVELDYPRGKEQSKEMKAANEKLAQQ
                     YGVRGFPTVVFADASGKPFGGFVGGRSGDDVMKAMQDALKNKEALSAAEANVAKAATD
                     EAKVAALMEVLKLAPQEYVDNFYGDVKAEIKRLDKEDKSGLKAADAHADLLKKEQKDV
                     QNYLAGKLTDKTTPAEALQVVKAYPDRDKLLPETQQQLLMMEFGTYLNSTG"
     gene            1021..2250
                     /locus_tag="PK077_01940"
     CDS             1021..2250
                     /locus_tag="PK077_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HRN23091.1"
                     /translation="MNTVLIIGAGGVGHVVANKCAQLPDIFQSIHLASRTKSKCDAVA
                     EDVRSRTGVSITTHSVDADDVTATVALIREIKPQLLINVALPYQDLTLMEACLETGVN
                     YLDTANYEPRDVAKFEYSWQWAYQEQFRNAGLFALLGSGFDPGVTNVFTAWALKHHFD
                     EIHTLDIIDVNGGNHGKAFATNFNPEINIREVTAPCRHWEDGAFRETAPMSMHRSFAC
                     PQEVGTYEIYRMYHEEMESLVKHIPTIRRAQFWMSFSPNYLKHLEVLQNVGMTRIDPV
                     MYNGVEIIPLQFLKAVLPDPGDLGKTTRGKTCIGNVITGIKDGQFKAVYIYNICDHEQ
                     CFKEVGSQAISYTTGVPAMIGAEMILTGKWTGAGVFNMEQNDPDPFMDELNRRGLPWH
                     CVELTEEQAKILKVNQQ"
     gene            2480..3421
                     /locus_tag="PK077_01945"
     CDS             2480..3421
                     /locus_tag="PK077_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRN23092.1"
                     /translation="MFLLQPALAEDTAPWKIAIIGDTHDSPKRMEGSEGVAVNFIKTL
                     YGEILKHNVDMVVQVGDMADIEGSAPVKGLAKRKELNNILREKGIPFYAVRGNHDSLP
                     FRAEQFRELFLPTRRQGVQGLATRKLNYGIRHKNASLYFMDIDLTPDQLVDFSAWVKR
                     NRSKANTVPRHCLVFTHRTLQTPMQFRECLWGRYNDSAAEQQNIFYRNLRDAGVRFVI
                     TGHLHAHDLYIITSPDGKNTLTSLICAPAGNKVLPIPFLPPAKSRVKTLQYRSGITAY
                     YILTIYPDSMTLDTYAAPNNGITDEGPQSGEFYKLNS"
     gene            complement(3547..4473)
                     /locus_tag="PK077_01950"
     CDS             complement(3547..4473)
                     /locus_tag="PK077_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23093.1"
                     /translation="MNPFVKIMLLTVCSTGILCGEGKPALFRLPLPDSISAFKTSPQE
                     KKEFLSLLHEKELPDCCREARKRLGAFSSKGAGDVNAEDVRVLSWIFYAVSSMDVFPL
                     NYDENTPKDGLCDNMDYNLKVRVVEWMSVLSRDISGIVSRCGVKRKELCRIWSEYGAA
                     VLKTFRTQYDPELKRKQARMKEEYDKMSAARTWELVQAGKLSLFGGGDSRSLYYNNKL
                     LANEKRNSLCRFFLEKRLEPAFVEMLVRFYPGQAGEVVKYLKKAGYADQEIGDLIDRT
                     VGRDGKTEFLYKGGRGRQHDRKIKSRGSGHMK"
     gene            complement(4457..5296)
                     /locus_tag="PK077_01955"
     CDS             complement(4457..5296)
                     /locus_tag="PK077_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23094.1"
                     /translation="MGNTDDRKADRERKTIGVGERITLTLTGKPLGNVDELEWNLVED
                     GDKYVEMPEETKGKISIVVTAKKRLQRDGSATVLAVTSEGRKVKVTLNVVVPSAIRAE
                     HKNGGMATERLPEGKTSVIGASAVLKLTLHPTSVSFKNVKIIERDKGSEPPGTDFDPG
                     HSRNGANEAAEVSETNQLQDNVMAGIFAADVEHARFPQSWDWVCSWRIHDGEGGEGTE
                     YLDGREIMEVRCHFSFSEFYRQEIWGPQRYHLVTISKFGCCVDRDTVTYRNKYYYHHE
                     SVR"
     gene            complement(5521..6909)
                     /locus_tag="PK077_01960"
     CDS             complement(5521..6909)
                     /locus_tag="PK077_01960"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HRN23095.1"
                     /translation="MMSPRTSRVWFWALTAAALLYTALLSWWSPLTSDTYHHALTGME
                     HCFSFSLVWERCVASYMTWNPRIGEYLAFAVATAGKWLFMLLNPFVQVGLALMMFYLA
                     SGRRVDPRSWPDVRLFGLGLLLLFTCTARPGVTIYWLSGATNYSWGAAVWLGFLCLYR
                     GLLEDAESGRKGDAGRNNSWGKFAAAAVLGFPAGMTNENNIPGTWLLLGALFVFVRLV
                     RKEKLPLWFYAGLAFQVAGSLCMLLAPGISARMHSATPGCAEPLSGFWSRWEALPSLL
                     LRMHEYLALPVLLGVAAAWVLWKKFHRDRNSFRAWKIPFGIAAAYILASYAMALSFAA
                     AVIPADHAMFSATLLFGIGVLALLRMWYGIPGAVPRPQITVAVFLALSGICVFSTIHD
                     HLLLFGQHEKRVRLILEQKKAGIQEIIVPPLSPPSPWCSFIFWVDLSQNPEDYVNRGA
                     AMYYGVKSIRIF"
     gene            complement(6909..8354)
                     /locus_tag="PK077_01965"
     CDS             complement(6909..8354)
                     /locus_tag="PK077_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23096.1"
                     /translation="MLEISPQGPSGLSGKICLCGIGAAGTKVMEEVLLLAPQSASVCA
                     MNLDARLLNASSVPCKIHLGARLTRGLGSGGDASVGAQAACESESSILRALEGSALAV
                     LAAGLGGGTGSGVAPEAARLAKEQGAYVVSVVIRPFRFEGERRSAQADEALARLALYS
                     DMVLRFDNDAMESLIDPDKGVLEAFSVVNALIARAVLIVPSLLNSSGNLLRVGLDDLL
                     SVAGTGKGICSFGVGEASADASVADILNQVRHSPLFLEKRLGEVDDVLVLVRGGGSLT
                     LQRLEDLVDGVAEILGRGVRLHIGASVEQQPEERLSLTVLGAVPVAETSVSPSVVLQR
                     EAEPQTVLVPEEKSFLGEGKTIIQPAPPAPVDEPERHPEPDLSVPVSRRDERELEEEL
                     VPVRPVSDMPDAQETLLESAPVAQSSEMEAELESDTMPDIKELEEGLPPEEGEEERAG
                     HGLFARVRPLILDGEDLDLPPALRKRKPDPS"
     gene            complement(8380..9591)
                     /gene="ftsA"
                     /locus_tag="PK077_01970"
     CDS             complement(8380..9591)
                     /gene="ftsA"
                     /locus_tag="PK077_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRN23097.1"
                     /translation="MAKTKIHVGLEIGTSKTCMVVGEVKPDATVTIIGVGEVSSEGVV
                     RGEIEDTSKVIQCIYDAWNMAQDHADVDIMTVYLSVTGAHIVGQNNRGTFRLPPDESI
                     ISQEHMDEVTEIARDVALGPEQFVLHRVPGLFSVDGQENLTNPAGLTGRTLDIDCHII
                     HGIKSRITNSFRCVREVPLDIADVVFAPIATAQFVLNRQVKQAGALLIDMGAGTTDYV
                     LYLDGQLVASGCVPLGGDHISNDITLMTGIPLAQAELLKKTEGDANSFSGKTNEMVRV
                     RGEGHMKDAAIERNVLNEIIRSRLLEIFNLVKSSLPKDTFKGNRCHGVYLCGGASLMR
                     GVGELASHVFGVAISRPTLVKNGAPSYLDDPRYCTAIGLIRYAQILDAELPQQRSWLG
                     RMLGFFGKKTS"
     gene            complement(9707..10249)
                     /locus_tag="PK077_01975"
     CDS             complement(9707..10249)
                     /locus_tag="PK077_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HRN23098.1"
                     /translation="MTFAAYKTLSVAGALLAGLFLSSCSSQQQVEAPARLPAYHFVPG
                     KTAVLHNGKAIPPRNAPSQVKRAIAAANSLVNKPYRLGGGHRRHHDTHYDCSGSTSFV
                     LKEAGLLTSTRHSRLFLNYGQKGAGDWISIYAKNGHVFMVICGLRFDTTGSGRRGEGP
                     RWRVDGRNTRHFLVRHPTGL"
     assembly_gap    10344..10386
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10387..12327)
                     /locus_tag="PK077_01980"
     CDS             complement(<10387..12327)
                     /locus_tag="PK077_01980"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HRN23099.1"
                     /translation="MMYYSASMNTLDESLMNAVKEGKLLESSRTNIRLLLDGTKSPIA
                     REAVEELAAAGEWQELNDRFYKTLAFGTGGLRGRTIGSIVTRAEQGNGGVNGRPEYPC
                     VGTACMNYFNVGRAMRGLIIYVNKHVEATDPGRKPRLVIGHDTRHFSRDFAEFCAKIG
                     TDLGCDIYLFDSPRATPEISFAIRELHADSGVVLTASHNPSHDNGFKAYFNDGAQLVP
                     PHDKAVIREVNSLTSEEYEPLPEDRRGTLHVLDSSFDRIYMDRLKTVLLRPELFNKGG
                     AKIVYSNLHGTGGHIIVPLLKELGCNVQTVAAQDVQDGRFPTVASPNPENPPALALAI
                     EQADASGADIVIATDPDADRMGVAVRGEDGKMHLLTGNQIGSLLAWYRCMSMSDLGII
                     NDSNRSRAVMVKTFVTTGLQDAIGHHFGYEVVNVLTGFKYIAQKLGKYEQAIPAEKRE
                     GYRKMSEEQTRALRLEYSRYFVFGGEESYGYLAQDFVRDKDANSAAIIFAELAAYAES
                     IGKSLLELLHELFEQFGVYLEMGKSLVMEGADGAARIAALAASYSSNPPTEVDGVAVS
                     GIRDFSKGDMVDAEGDPIPAEKMIFVDLADGRSFAVRPSGTEPKIKYYLFGHGKPGAP
                     VKEALTKVQASLDSLWTAVEKDA"
     gene            complement(12432..14216)
                     /locus_tag="PK077_01985"
     CDS             complement(12432..14216)
                     /locus_tag="PK077_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN23100.1"
                     /translation="MLRQFFEYARYLKPVMKLFIAALAAGALAAAASGFGFPLMIAKV
                     FPVVFDNTQIPPELQDMIARLVAPEHMRMAVLLAVCSLLPLVFAVRGIGTFFNVYWIS
                     IVSMKVLEAIRLDAFSKLQTLPLSFIDRQKRGDLISRLINDAANVQGGLVVAANDIIK
                     QPLTLLTALAFLVYKVFVDPNTALVLMNLGLIALAIWPIRFFGRRVMKKSKQAQDELG
                     NITAAAQENLASQREIRSYGMQEQQVNLFLGLIRRFFKINLRTVKYRHFLVPVLEMVT
                     ALGLGVLLVRGHEMGITKADFTALAAALFMCYDPLKRLGVTLTNLKSAYASLERINYL
                     LKEPDTMPEAVDPVPMGRAAGRLDYDGVSFSYDGARKVLDGINVHIRPGEVVGLVGPS
                     GAGKTTFASLLPRFYDVSSGALRVDGVDVRDASLHDVRKNIAFVSQHPVLFHGTIMEN
                     IRLGRQDATDDEVVAAARAAAADGFIMGMPDGYQTMLGDGGSGLSGGQRQRVAIARAF
                     LKDAPILILDEATASLDAESEALIQQELDRLVEGRTALIVAHRFSSIRVATRILVFEG
                     GRIVGDGSHAELYASCPLYRELYDRQSL"
     gene            complement(14210..>15019)
                     /locus_tag="PK077_01990"
     CDS             complement(14210..>15019)
                     /locus_tag="PK077_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN23101.1"
                     /translation="NAPDDVPEPENPRSPAAWKGEVEFRNVCFGYQQDSPVMRNIDLR
                     IPSGQVVALVGPSGAGKTTFINLLCRFYDVLSGSVKVDGIDVREMSKKELLSHIALVS
                     QYPVLFRGSVADNIRIGRPDASDAEVEEAGRMSAVDSFIHETGEGYSRMIGEMGEGLS
                     GGQRQRVSLARAFLKNAPILVLDEATASLDMKSEEQIQKEIEKLASGRTTFIIAHRFS
                     TIRMADRILVLEKGSVVADGTHEELMASSPLYRELYNKQQMIAEEEGGASC"
BASE COUNT         3435 a         4533 c         3864 g         3144 t
ORIGIN      
        1 tcaccggtgg aattcagata ggtcccgaac tccatcatca gcaactgctg ctgcgtctcc
       61 ggcagaagct tgtcacggtc gggatacgcc ttaaccacct gaagggcttc cgcaggcgtc
      121 gtcttgtccg tcagcttgcc ggcaagataa ttctgcacgt ccttctgttc ctttttaagc
      181 aggtcagcat gggcgtctgc ggctttcaag ccggacttgt cctctttgtc cagcctcttg
      241 atttccgctt tcacgtcacc ataaaaattg tccacatatt cctgaggagc cagcttgagc
      301 acttccatca gagcggcaac cttcgcttca tccgtagcag ccttggcgac attcgcttcc
      361 gcggcgctta gcgcttcctt gttcttcagg gcatcctgca tggctttcat cacatcatcc
      421 ccggaacgcc ctccaacaaa tcccccaaaa ggcttgccgg aagcgtccgc aaacacaacg
      481 gtcgggaatc cccgcacgcc gtactgctgg gcaagctttt cattggcggc tttcatttcc
      541 ttggactgtt ccttgccgcg cggataatcc agctccacca gaacgaaatt tttctgtgct
      601 tccttctgga agtccggctt gctgaatacg ttggcgcgga gctgcatgca gggagggcac
      661 cagtcggaac cggtgaactc aatcattaaa tcctttttct gttcggcagc ctgttttttg
      721 gcggcatcca tatcggtaat ccacccttcc gcggcaaaac agggagcgac gcaggcggca
      781 acaactactg gcagaatttg tctaatcata tctacaattt gatattgtaa aatcggacag
      841 cccgcgtcaa tcccctgttc aatcattttt agaaaatctc caaaagggtg cgtcttccgc
      901 agggaagtcc gcatccggct gatgccggaa aaactgatgc cccatgggaa gacggtgtca
      961 ttattgaagc aaatcgggag ttgcctttca gcctgttttc acttatgtac tgaacctatc
     1021 atgaatacgg ttttaatcat tggagcaggc ggcgttggac atgtggtagc caataaatgc
     1081 gcccagctgc cggacatttt tcaaagcatt catctggcct cccgcacgaa aagcaaatgc
     1141 gatgccgttg cggaggacgt acgctccaga acgggcgtca gcatcactac ccattccgta
     1201 gatgcggacg acgtgaccgc caccgtggcc ctcatccggg aaatcaagcc gcagctgctg
     1261 atcaacgtgg ccctgcccta ccaggacctc accctgatgg aagcctgcct ggaaaccggc
     1321 gtcaactacc tggataccgc caactatgaa ccccgtgacg tagccaaatt cgaatactcc
     1381 tggcaatggg cgtaccagga gcaattccgc aacgccgggc ttttcgccct gctgggttcc
     1441 ggctttgacc ccggcgtcac caatgtcttc acggcgtggg ctctcaagca tcactttgac
     1501 gaaatccata cgctggacat cattgacgtc aatggcggca atcatggcaa ggctttcgcc
     1561 accaacttca acccggaaat caacatccgg gaagtaacgg cgccctgccg ccactgggaa
     1621 gacggagcct tccgggaaac cgcccccatg agcatgcacc ggagtttcgc ctgtccgcag
     1681 gaagtgggca cctatgaaat ctaccgcatg taccatgaag aaatggaaag cctggtcaag
     1741 cacatcccca ccatccgccg ggctcaattc tggatgtcct tctcaccgaa ctacctcaag
     1801 cacctggaag tgcttcagaa cgtgggcatg acccgcattg acccggtcat gtacaacgga
     1861 gttgaaatca tcccgctgca attcctcaag gccgtactgc cggaccccgg ggatctgggc
     1921 aagacgacca ggggaaaaac ctgcatcggc aacgtcatca ccggaatcaa ggacggccaa
     1981 ttcaaggctg tgtacattta caacatttgc gaccatgaac aatgctttaa agaagtaggt
     2041 tcccaggcca tttcctatac tacgggggtt ccggccatga tcggcgcgga aatgatcctt
     2101 acgggcaaat ggactggcgc aggagtcttc aatatggaac aaaatgaccc ggaccccttc
     2161 atggacgagc tcaacaggcg cggcctcccc tggcactgcg tggaactcac ggaagaacag
     2221 gccaaaatac tgaaggtcaa ccagcaataa atttcagcca gcgctgtagg ccccctcaaa
     2281 gccggacatt gaatttccaa tgtccggctt ttccgttttc agcggggaac agaattcccc
     2341 aggacagcac gtcaacgcct gcctcgggaa aaagaaaaag ccccggacgg catctttccg
     2401 cctcagtcat aattttctgg cgggaagagc aggcaggttg taaaaatcca gtgccatgaa
     2461 atatcttgcg ttatttctca tgttcctgct tcagccggca ctggcggaag atacagcccc
     2521 atggaaaatc gcgatcatcg gcgatacgca cgactccccc aagcgcatgg aaggaagcga
     2581 gggagtagca gtcaacttca tcaaaaccct ctatggggaa atcctgaaac acaacgtgga
     2641 catggtcgtc caggtagggg acatggcgga tatcgaagga agcgcccccg tcaagggact
     2701 cgccaaacgc aaggaactca acaacattct gagagaaaaa ggcatcccct tttatgccgt
     2761 gcgcggcaat catgactccc ttcctttccg ggcggaacaa ttccgggaac tcttcctgcc
     2821 tacgcggagg cagggcgtcc aagggctggc caccagaaaa ctgaactacg gtatccgcca
     2881 taaaaacgct tccctgtatt tcatggatat tgaccttact cctgaccagc tcgtggactt
     2941 cagcgcatgg gttaaacgca acagaagcaa ggccaacacc gttccgcgac actgcctggt
     3001 tttcacccac cgcaccctgc agacgcccat gcaattccgg gaatgcctgt ggggccgtta
     3061 caatgacagc gcggcggaac agcaaaatat cttttaccgc aatctccggg atgccggagt
     3121 gcgcttcgtc atcaccgggc acctgcacgc ccatgacctg tatataatta cctccccgga
     3181 cgggaaaaac acccttacct ccctgatctg cgcccccgcg ggaaacaagg tgctccccat
     3241 tccctttttg ccgccggcaa aatcccgggt aaaaaccctg caataccgat ccggcattac
     3301 ggcctactat atcctgacca tttacccgga ttccatgacc ctggacacct acgccgcccc
     3361 caataacggc attaccgacg aaggccccca aagcggagag ttctacaaat tgaactccta
     3421 gaacatacaa cttcccctgt ttcttccata acgcggcatc ccggaatttg cctgctcccg
     3481 tccgctatgc accctggcgt ccctggcgaa acaatgaaat ttccgggaag ttcccaatcc
     3541 cccatgttat ttcatatgcc cggagccacg ggacttgatt tttctatcgt gctggcgccc
     3601 ccggccgccc ttatataaaa actccgtttt tccatcgcgg cctacagtcc ggtcaatcaa
     3661 gtctccgatt tcctgatcag cgtacccggc cttcttaaga tacttaacca cttcaccagc
     3721 ctgccccgga taaaaacgaa ccagcatctc cacaaacgca ggttccaatc tcttttccag
     3781 aaaaaatctg cataaactgt ttcttttttc attagccaac aacttattat tgtagtagag
     3841 gctgcgggaa tcacctcctc cgaataagct cagcttcccg gcctgcacca actcccatgt
     3901 tctggcagca ctcattttat catattcctc cttcatcctg gcctgtttcc gctttaattc
     3961 cggatcatac tgagtcctga atgttttcaa tacggccgct ccatattctg accatattct
     4021 gcacaactcc tttctcttta cgccgcatcg ggaaacgatt ccggatatat cccgggataa
     4081 aacgctcatc cattccacaa ccctaacctt cagattataa tccatgttgt cgcacaagcc
     4141 gtccttcgga gtattctcat cataattcag aggaaataca tccattgaag agacagcgta
     4201 aaaaatccat gacaacaccc tgacgtcttc tgcgttcaca tctccagctc ccttggaact
     4261 aaaggcgcca agcctcttcc gcgcctcccg gcaacaatcc ggcagctctt tttcatgcaa
     4321 cagggaaagg aactccttct tttcctgagg agacgttttg aacgcggaga tgctgtcggg
     4381 caacggaagc cggaacaggg caggctttcc ttcaccgcac aggatgccgg tggaacagac
     4441 ggttaacaac attattttaa cgaacggatt catgatgata gtaatatttg ttgcgatagg
     4501 ttacggtatc ccgatccaca cagcatccga atttagaaat cgtcacgaga tggtatcgct
     4561 gcggccccca gatttcctgc ctgtaaaatt ccgaaaaact gaaatggcac cgaacttcca
     4621 tgatttcgcg gccgtcaaga tattccgttc cctctccccc ttctccatca tggatgcgcc
     4681 acgaacatac ccagtcccac gactggggga aacgggcatg ctccacatct gcggcaaaaa
     4741 tgccggccat tacattatcc tgcagctgat ttgtttctga aacctccgcc gcttcattcg
     4801 caccattcct ggaatgaccg ggatcaaaat ccgtaccggg aggctccgag cccttgtccc
     4861 tctctataat cttgacattt ttgaagctga cgctggtcgg atgcagcgtc agcttcagga
     4921 cggcagaggc ccctatgacg gacgttttcc cctcaggaag cctttccgta gccattcctc
     4981 catttttgtg ttcggctctg attgccgacg gaacaaccac atttagtgtc acctttactt
     5041 ttctcccctc actggtaaca gccaggacgg ttgcggaacc atcacgttga agccgttttt
     5101 tcgcagtaac cacaatggat attttaccct tcgtttcttc aggcatttct acgtatttat
     5161 ctccatcttc caccaaattc cattccagtt catcaacatt tcccaacggt tttcccgtca
     5221 gggtcaatgt tatccgttcc cccacaccta tcgtctttct ttccctgtca gccttccggt
     5281 catcagtatt tcccaaatat atatcagcgg aatattcctc tgtttccgtt tcaatcctca
     5341 acgcgccgga atcctcttcc tccacgtccg cataggaaag accgcacagg aaaactgcgc
     5401 aacctattaa aaggattctt atattgttaa caacaactgc catcagacgc agatgatatc
     5461 ttattgccat ttcccattat ttcatagcaa aaagctctaa cattccgcat cctttgcctg
     5521 ctaaaagatt ctgatgctct tcacgccata atacatcgcc gcgcctctgt tgacgtagtc
     5581 ttccgggttt tgcgacaggt ccacccagaa aatgaaagaa caccacgggg agggaggaga
     5641 gaggggagga acaatgattt cctgaatacc ggcctttttc tgctccagaa tcaggcggac
     5701 gcgcttttca tgctgcccga acagaagcag atgatcatgt atggttgaaa aaacacatat
     5761 tccggacaac gccagaaaaa cagccacagt tatctgcgga cggggcacgg caccggggat
     5821 gccgtaccac atacgaagaa gggccagcac gccgataccg aacagcaggg tggcggagaa
     5881 catggcatga tccgccggaa tgacggcggc ggcgaaagaa agagccatgg cataagacgc
     5941 cagaatatat gccgccgcta ttccaaaggg aattttccat gcccggaaag agttccggtc
     6001 tctgtggaat tttttccaga gaacccaggc agccgccacg cccagcaaca ccggaagagc
     6061 caggtattca tgcatccgga gcaggagcga aggcagagct tcccatctac tccaaaaacc
     6121 ggaaaggggc tccgcgcagc cgggagtggc ggaatgcatc cgggcggaaa tgccgggcgc
     6181 aagcagcatg cacagggaac ctgccacctg aaacgcaagg cccgcataaa accacagggg
     6241 aagtttttcc ttccgcacca ggcggacaaa gacgaacaag gctcccagca acagccaggt
     6301 tcccggaata ttgttttcat tggtcatgcc cgccggaaat cccagaacag ccgcagcggc
     6361 aaactttccc cagctattgt ttcttccggc atcccctttt ctcccggatt ccgcgtcttc
     6421 cagcaaaccg cggtacagac acagaaagcc cagccagacg gcggcgcccc aggaatagtt
     6481 ggtggctccg gagagccagt aaatcgtgac gccgggccgg gccgtgcacg tgaacagcag
     6541 gagaagcccc aatccgaaca gacgcacgtc cggccaggaa cggggatcca ccctgcgccc
     6601 ggaagccaga taaaacatca tcagcgcaag accgacctgc acaaaggggt tgagcagcat
     6661 gaagagccat tttcccgccg tcgctacggc aaaggccagg tattccccta ttctgggatt
     6721 ccaggtcatg taggaggcta cgcagcgctc ccagaccagg ctgaaagaga agcagtgctc
     6781 catgcccgtc agggcatgat ggtacgtgtc agaagtgaga ggactccacc agctgagcag
     6841 cgccgtatat aataaggcgg ctgcggtcag cgcccaaaac cacaccctgg acgtgcgggg
     6901 ggacatcatc agctcggatc cggtttcctt ttccgcagag cgggaggcag atccaagtct
     6961 tccccgtcca gaatgagagg acgcacacgg gcaaaaaggc cgtggcccgc acgctcttct
     7021 tccccttcct ccggaggcaa gccctcttcc agttccttaa tatcaggcat cgtgtctgat
     7081 tccaactccg cctccatttc ggaactttgc gcaacaggcg cggattccag aagcgtttcc
     7141 tgcgcatccg gcatatcgga gacaggcctt acaggcacca gttcctcttc caattcacgt
     7201 tcatccctcc tggagacggg gacggaaagg tccggctccg gatgccgttc cggttcgtca
     7261 accggggcag gaggagcagg ctgaataatg gttttcccct ctccgaggaa agatttttcc
     7321 tccggcacaa gcactgtctg cggctccgct tcccgctgaa gcacaacaga cggggaaaca
     7381 gaggtctctg ccacaggcac ggcgcccagc acggtcaggg acaggcgttc ttccggctgc
     7441 tgctccacgg aagcgccgat gtggaggcgc actccccttc ccagaatttc cgccacaccg
     7501 tccaccaggt cttccagacg ctgcagcgtc agggaacctc cgccgcgcac cagaaccagg
     7561 acatcgtcca cttctcccaa acgtttttcc agaaaaagcg gggaatgccg gacctggttc
     7621 aatatatccg ccacggaagc atccgcagac gcttcaccca cgccaaagga gcaaatccct
     7681 tttcccgttc cggctacgct gagcaggtcg tccaatccca cccggagcag gtttccggaa
     7741 gaattcaaca gagacggcac aataagcacg gcgcgggcaa taagggcatt aaccacggaa
     7801 aaggcttcca gcaccccctt gtcagggtct atgaggcttt ccatggcgtc attatcaaac
     7861 cgcagcacca tgtcggaata cagggcaagc cgggccaaag cttcatccgc ctgggcagag
     7921 cgccgttccc cctcaaacct gaaggggcgg atgacaacgc tgacgacata agccccctgt
     7981 tccttggcca gacgggccgc ttccggagct acgccggatc ccgttccccc tcccagcccg
     8041 gcggcaagga cggccaacgc gctgccttcc agcgcacgga gaatggaaga ctcgctttca
     8101 caggccgcct gggctcctac ggaggcgtcc ccgccggaac cgaggcccct ggtaagcctg
     8161 gcgcccagat gaatcttgca cggaacggat gaagcgttca ataggcgggc atcaagattc
     8221 atggcgcaaa cggaagctga ctggggagcc agcagcagca cttcctccat taccttggtt
     8281 cctgcggccc caatgccgca caggcagatt tttccggaca ggccggacgg cccctgggga
     8341 gagatttcca gcatggttgt taatttggat aaaggttgat caggaggtct tctttccgaa
     8401 gaagcccagc atccgtccca gccagctgcg ctgctgcggc agttcggcat ccagaatctg
     8461 ggcataccgt atcaggccta tggccgtgca gtagcgcgga tcatccaggt aggaaggcgc
     8521 accgttttta accagcgtag gccggctgat ggccacgccg aacacgtggg aggccagttc
     8581 ccccacgccg cgcatcaggc tggcgccccc gcacaggtaa acgccgtggc agcggttgcc
     8641 cttgaaagta tccttgggca gggaagattt gaccaagttg aaaatttcca gcaggcggga
     8701 acggatgatt tcattcagca cgttgcgttc aatggcggca tccttcatat gcccctctcc
     8761 gcgcacgcgc accatctcgt tggttttccc ggaaaagctg ttggcgtctc cttccgtctt
     8821 cttgagcagt tccgcctggg ccagaggaat gccggtcatc agcgtgatgt cattggaaat
     8881 gtggtcgccg cccaggggca cgcatccgga ggccacaagc tggccgtcca ggtaaagcac
     8941 atagtccgtc gtgcccgcac ccatgtcaat aagcagagcg cccgcctgct tgacctgcct
     9001 gttgagcaca aactgggccg tggcaatggg ggcgaaaacc acatccgcaa tatccagcgg
     9061 gacttcccgc acgcaacgga aggagttggt gatgcgggat ttgattccgt ggatgatatg
     9121 gcagtcgatg tccagggttc ttccggtcaa tccggcggga ttggtcaggt tctcctgccc
     9181 gtccacggaa aagagaccgg gcacgcggtg caggacgaat tgttccggcc ccagggcgac
     9241 gtcccgggcg atttccgtca cctcgtccat atgctcctgg gaaataatgc tttcatccgg
     9301 gggaagacgg aacgttcccc ggttgttctg ccccacaata tgggcgcctg tcacggacaa
     9361 atagacggtc atgatatcca cgtccgcatg gtcctgggcc atattccagg catcgtaaat
     9421 gcactggatg accttggaag tgtcttcaat ctctccgcga accacgccct cgctagaaac
     9481 ctcacccacg ccgatgattg tcacggtcgc atcgggcttc acttcgccca cgaccatgca
     9541 agtcttgctt gtccctatct caagccctac gtgaattttt gtcttagcca tgaaaatcaa
     9601 ggaactatca acgtcctcgc acctgatagc acattacttt ccattattca agaaagcctg
     9661 cgcgaacata cgcagaaaac gcggttttcc tccgcgctgc cccggttcaa aggcctgtgg
     9721 gatggcgcac caggaagtgc ctggtattgc ggccgtccac tctccagcgg gggccttcgc
     9781 cgcggcgccc gcttcccgta gtgtcaaacc ggaggccgca gatgaccatg aagacgtgtc
     9841 cattcttggc ataaatggaa atccagtccc cggcgccttt ctgcccgtaa ttcaggaaaa
     9901 gcctggaatg gcgggtggac gtaagcagac cggcttcctt cagcacaaag gaagtgctgc
     9961 cggaacagtc ataatgggta tcatgatgcc tcctgtgccc cccgcccagc cggtacggct
    10021 tgttgaccag ggaattggcc gccgcaatgg cgcgcttgac ctgcgaaggg gcatttctgg
    10081 ggggaatggc tttcccgttg tgaagcacgg ccgtcttgcc gggcacaaag tggtaggcgg
    10141 gaagacgcgc aggagcttca acttgttgct gggaggagca ggaagagaga aaaaggccgg
    10201 ccaaaagggc gccagccacg gagagagttt tgtaggccgc aaatgtcatg agaattataa
    10261 actgcggaat tcccgcctcc ttggcaagaa aataaatttc ccccgtccac attacatggt
    10321 agacggggga aagaaatcag caannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10381 nnnnnnggca tctttttcca cggccgtcca cagggaatcc agggaagcct ggactttcgt
    10441 caatgcttcc ttcacaggag cgcccggttt cccgtgcccg aagaggtagt atttaatctt
    10501 gggttccgtt ccggaggggc gcaccgcaaa actgcggccg tccgccagat ctacgaaaat
    10561 cattttttcc gccggaatag gatcgccttc cgcatccacc atgtcgccct tggaaaagtc
    10621 ccggatgccg ctgacggcta cgccgtccac ttccgtggga ggattggagg aataggaagc
    10681 ggcgagggcg gcgattctgg ccgcgccgtc cgcgccttcc ataaccagcg attttcccat
    10741 ttccaaatac acaccaaact gctcaaagag ttcatgcagc agttccagaa ggctcttgcc
    10801 gatgctttcc gcatacgcgg ccagttccgc aaagatgatg gcggcggagt tggcgtcctt
    10861 gtcccgcacg aagtcctggg ccaggtagcc gtagctttcc tccccgccga agacaaagta
    10921 acggctgtat tccagacgca gggcgcgcgt ctgttcttcg ctcatcttgc ggtagccctc
    10981 ccgtttttcc gccggaatgg cctgttcata tttaccgagt ttctgggcaa tatacttgaa
    11041 ccccgtgagg acgttgacca cttcataacc gaagtggtgg ccgatggcgt cctgaagccc
    11101 cgtcgtcaca aaggttttca ccatcacggc gcgggaacgg ttggagtcat tgatgatgcc
    11161 aagatcggac atgctcatgc agcggtacca cgccagcagg gaaccgatct ggttgccggt
    11221 cagcaggtgc atctttccgt cttccccgcg cacggccact cccatgcggt ccgcatccgg
    11281 gtccgtggcg atgacgatgt ccgctccgga agcatccgcc tgttcaatag ccagggccag
    11341 agccggcgga ttttccgggt tgggggaagc aaccgttggg aaacggccgt cctgaacatc
    11401 ctgcgccgcc acggtctgca cgttgcagcc cagttccttc aacaggggca cgatgatatg
    11461 gccgcccgtc ccgtggaggt tggagtaaac gattttagct ccccccttgt tgaaaagttc
    11521 cggacggagc agcaccgttt tcaagcggtc catgtaaatg cggtcaaagg aggaatccag
    11581 cacatgaagg gttccgcgcc ggtcttccgg caacggttca tattcttcgg aagtcaggga
    11641 attgacctcc ctgatcacgg ccttgtcatg cggaggcacc agctgggctc catcattgaa
    11701 gtaagccttg aaaccgttgt catgggaggg gttgtggctg gcggtcagca ccacgccgga
    11761 atccgcatga agctcccgga tagcaaagga gatctcagga gtggcgcgag gactgtcaaa
    11821 caggtaaatg tcacatccca ggtccgtgcc gatttttgcg cagaattcgg caaaatcacg
    11881 ggaaaagtgg cgcgtgtcgt gtccgatgac caggcggggc ttcctgcccg ggtccgtagc
    11941 ctccacatgc ttgttcacat aaataatcag gccacgcatg gcccgcccca cattgaagta
    12001 gttcatgcag gcggttccca cgcagggata ttccgggcgg ccgttcacgc cgccgttccc
    12061 ctgctcggcc cgggtgacga tgctgccgat ggtacgtcct cgcagccccc ccgtgccgaa
    12121 ggccagcgtt ttgtaaaaac ggtcgttcag ctcctgccat tccccggcgg cagccaattc
    12181 ttctacggcc tcgcgcgcta tgggggattt ggtaccatcc agcagaaggc ggatattggt
    12241 ccgggaagac tccaaaagct tcccttcttt aacggcattc atcagggatt cgtccagtgt
    12301 attcatgctg gcggaatagt acatcatgcc ccttgtttga caatagctct ttccatcatg
    12361 gcctgagaaa aaacgccata ttcatcccgg tgcgggcgcg ggaaatgctt tcctccccgt
    12421 ccccttgtta tttaaaggga ctggcggtca tacagctccc ggtacaacgg gcaagaggca
    12481 taaagctctg catgggaacc gtccccgacg atgcgcccgc cctcaaacac cagaatgcgc
    12541 gtcgccacgc ggatggaact gaaacggtgg gccacaataa gcgccgtcct tccctccacc
    12601 agccggtcca gttcctgctg gatcagggcc tcgctttccg catccaggga cgcggtggct
    12661 tcatccagaa tcaggatggg agcatccttc aggaaagcgc gggcaatcgc tacacgctgc
    12721 cgctggccgc cggacaatcc gctgcctccg tcgccaagca tggtttgata gccgtccggc
    12781 atgcccatga tgaaaccgtc cgcagccgcg gcgcgcgccg cggccaccac ctcatcatcc
    12841 gtagcgtcct gacgtcccaa acggatattt tccataatgg tgccgtggaa caggacgggg
    12901 tgctgggaga cgaaggcgat attcttgcgc acatcatgca gggaagcgtc cctcacatcc
    12961 acgccatcca cgcgcagggc tccggaacta acgtcataaa acctggggag aaggctggca
    13021 aatgtcgtct ttccggcgcc gctgggccct accagcccca ctacttcccc ggggcggata
    13081 tgcacgttga tgccgtccag caccttgcgg gcgccgtcat aggaaaagga aacgccgtca
    13141 taatccaggc ggccggcggc gcgtcccata ggaacgggat caacggcctc cggcatggta
    13201 tccggttctt tcaggaggta attgatgcgc tccagggagg cataagcgct cttcaaattg
    13261 gtgagcgtca cgcccagccg tttgagaggg tcgtaacaca tgaacagggc ggcggccaaa
    13321 gcagtgaaat cagccttggt gatgcccatt tcatgccccc ttaccagcaa aacgcccagg
    13381 cccagtgccg tcaccatttc cagcacggga accaggaagt gcctgtactt caccgttctg
    13441 agattgattt tgaaaaaacg ccggatcagc cccaggaaaa gatttacctg ctgttcctgc
    13501 attccgtagg aacggatttc acgctgggag gccaaatttt cctgggcggc ggccgtaata
    13561 ttgcccagtt cgtcctgagc ctgtttggac ttcttcatca cgcggcggcc gaaaaagcgg
    13621 atgggccata tggccagcgc aatcagcccc agattcatca ggaccaaagc cgtattcgga
    13681 tcaacaaata ccttgtacac caggaaggca agggcggtca gcagagtcag gggctgcttg
    13741 atgatgtcat tggccgcaac caccagcccc ccctgcacgt tggcggcgtc attgataagc
    13801 cggctgatca ggtctccccg tttctggcgg tcaatgaagg aaaggggcag cgtctgcaat
    13861 ttggaaaagg cgtccagcct gattgcttcc agcactttca tgctgacgat gctgatccag
    13921 tacacgttga agaacgtgcc tatgccccgg acggcgaaga ccagaggcag cagggagcat
    13981 acagccagca gcaccgccat gcgcatgtgt tccggggcca ccagccgggc gatcatgtcc
    14041 tgaagctccg ggggaatctg tgtattgtcg aataccaccg gaaatacttt ggcaatcatc
    14101 agcggaaagc cgaaaccgct ggcggcggcg gccagagccc cggcagccag agcggcaatg
    14161 aacagcttca tcacgggctt caaataccgc gcatattcaa aaaactgtct taacatgagg
    14221 cgcctccttc ctcctccgca atcatctgct gcttattgta aagttcccgg tacaggggag
    14281 aagaagccat cagctcctca tgcgtgccgt ctgcaaccac gcttcccttc tccagcacca
    14341 gaatgcggtc cgccatccgg atggtgctga aacggtgggc aataatgaac gtcgtgcggc
    14401 cggaagccag tttctcaatc tccttctgaa tctgttcctc gctcttcata tccagggaag
    14461 ccgtagcttc atccagcacc aggatgggag cgtttttcag aaaggccctc gccagggaaa
    14521 cgcgctgccg ctggccgccg gaaagccctt cccccatttc tccgatcatg cgggaatacc
    14581 cttcccccgt ctcatggata aaactgtcca cggcggacat gcgcccggct tcttccactt
    14641 ccgcgtcgga agcgtccgga cggccgatgc gtatattgtc cgctacggag ccgcggaaca
    14701 gaacgggata ctgggaaacc agcgctatgt gggaaagaag ttccttttta ctcatctccc
    14761 tcacgtcgat gccgtccacc ttcacgctgc cggaaaggac atcataaaaa cggcacagca
    14821 aattaatgaa agtcgtcttc cccgctccgc tgggaccgac tagggccacc acctgcccgg
    14881 acggaatgcg gagatcaatg ttcctcatga caggagagtc ctgctggtaa ccgaagcaaa
    14941 cgttgcggaa ctccacttcc cccttccatg ccgccgggct ccttgggttt tccggttcgg
    15001 gaacgtcatc aggagcatt
//
LOCUS       DAOMSU010000023        14801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_157_length_14801_cov_2.915367, whole genome shotgun sequence.
ACCESSION   DAOMSU010000023 DAOMSU010000000
VERSION     DAOMSU010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 14801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14801
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_157_length_14801_cov_2.915367"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..2361
                     /locus_tag="PK077_01995"
     CDS             115..2361
                     /locus_tag="PK077_01995"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HRN23102.1"
                     /translation="MAGLFPAQSRGEEEAAQSGTIAVKVPASSLLMARQETGETRLDR
                     SFSNAGLSIGGKKYATGIGTHATSMIPLPVPENPKVLRLEGACGIDDGADGDGSVEFR
                     VMSGSEVLWSSGVMRRGMAAKKFSIPVAENGIRHLYLMADRVDNNSYDHADWVDLAWK
                     TAGSGQGMKGAVVNASEFGMVPGVRKDQGPALRAAVSALRRQGGGVLNIPRGIYHFYP
                     EGALNMSFHISNHDQPLIHPVCVPLADLRNVRVEGNGSLFLFHGKVVPLLVMDSENVS
                     INRLSVDYERSWCTEARVVKTDDRFTEVEIDKKAYPYEIRNNRFVFQGKGWEEGMGHC
                     MAFEKGTGHIIANTSDIGWNGHVEPLGGSRLRLSWNLRQKGIKPGDTLVLRNYNRPHP
                     GCVVYRARKTSLNDVSLHQSSGMALLVQRSEDFHMKGGGVMVRKGTGRVHTAGADATH
                     FSNTRGGIVVEKALFEGMMDDAINVHSTCLGVMEVVDSHTLKCKYMHRQAVGFEVFLP
                     GEKIRFINGPTLEPGGTATVKTAVKKNSAEMVITVEEPLPSSVRAGDAVENADFYPSV
                     VFRNNIVRNNRARGSLFTTPERVLVEGNLFDHSSGSAILLAGDAQGWYESGACHEVVI
                     RKNTFINNLTSRYQFTNAIISIYPEVKQLDRQRDYYHRNVLIENNVFKTFDVPLLFAI
                     STDNLKFINNKVIYNDEFKGWGQKPFQFRRCANILIKDNKVLPPRTWTLEDCKLENTP
                     SDQVRFGG"
     gene            2593..4026
                     /locus_tag="PK077_02000"
     CDS             2593..4026
                     /locus_tag="PK077_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23103.1"
                     /translation="MCRGQKRAAEGNDGGIKYWWILPFLSFFFSLNNQSFWIDECCTA
                     LCAMQQGMEGCWKKICEIGGSDAQMAFYYYLLFLWHHLTGAESEWMLRLFNIFWVFLS
                     SWFFRKEPKALVILLISPFFVYYSNELRPYMLQIAASCAVSMLFWQVSQGEPVKFHVF
                     FGSLFFLCLTSLTGVVWALGFAAAFMVMAFRQFGGRRFRRALLWWIFPFSGLGAYYLY
                     TLFLGARAVSISSSWIVNACASMYELSGLAGMGPSRLELRMCMTPDALWNMNGLGAGM
                     ISGAILLAGSACGIILWNKRAERPLVPALLVLILFPGAVFLYGTEMMDFRFSGRHCAP
                     LLPVLCLAWSLVASWDWSFPRIAQTILFLLMMIVWAVSDIRIRCNDLYGREDFRSAVS
                     YCKLLQSSHVDILLLCNGAGKEFYGWIPGPLKDKWSDYKVIVVSRPSDYAAFLQPIES
                     SGKYERSELCRGFVVYRRGGISQREGY"
     gene            4078..5004
                     /locus_tag="PK077_02005"
     CDS             4078..5004
                     /locus_tag="PK077_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HRN23104.1"
                     /translation="MMDISSRLVKRCRKFIEPYRVTDGRGFKLSRYDPGDLGKLGKDS
                     KKEAVDKLEKGIELLEQLQEVLYASESHALLVVLQAMDSAGKDGIVKHVMGGVNPQGC
                     VVSSFKQPSTLERSHDFLWRCVARLPRRGMIGIFNRSYYEEVLSVRVHPEFLAGEGFN
                     PSHAKSAFWEERFRSINNLERHLLANRTSIVKIFLNLSPEEQRKRLLARLDTPDKNWK
                     FSEGDIHEREFWDQYQKAYEEMIRHTSSREAPWYVVPADNKWYSRLVCMCAIVEALAE
                     MRLEYPKVSGELKEFFPKFREELERPLSRGGD"
     gene            complement(5109..5552)
                     /locus_tag="PK077_02010"
     CDS             complement(5109..5552)
                     /locus_tag="PK077_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23105.1"
                     /translation="MSWKLLFTTLSAALSLASCVSTPASRIQKNPALFNSLPPSQKTL
                     VESGQIAEGMSPPAVFLAWGDPSAVAEGHLNGKSATRWIYSSLQPVYTPPPSFWAGPY
                     WGGPYWGPGFYRPYYPYYNDVTYVPVNTGYVLFINGKVQSWERRR"
     gene            5626..6387
                     /locus_tag="PK077_02015"
     CDS             5626..6387
                     /locus_tag="PK077_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23106.1"
                     /translation="MKALRNLALTLIVPVLVSSLSAAEKPEIVDEYLPVGKMAEAAAV
                     SVVLDESLQPFMEKIDGVFASLPDKDKKELVGQIVPGQPVPYDERLGWTKDEYAKYLE
                     CWKLKQVQEVAPVALGIFASGERNIWDLAAVAQQGPLPMSTLKYDSSTKSWISPNGTL
                     TLKGDVSYDDLNVYGAWSGKEWTMEKKTILSTLTETIIAGKTKDGKYAYFVYNMSEKN
                     PDNVAIANQSIVLRVPVTRIAGDPLLEKAKAKARQ"
     gene            6424..6999
                     /locus_tag="PK077_02020"
     CDS             6424..6999
                     /locus_tag="PK077_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HRN23107.1"
                     /translation="MRIISGKAGGITLSVPKGEVRPTTDRVREAVFSILNPLMDRADV
                     LDLFAGSGAFGLEALSRGARDARMVDFSRLSCAAARANLVKTGLEGGTVIQGDAVQFV
                     RRELLAGRKYDIIFADPPYCKGPADRDFIVELAEAGVAGLLKGGGVFIAEVQEGWGTG
                     REGAAEIDGLNLVDTRRYGKNMLLFYQLPEK"
     gene            7001..>8126
                     /locus_tag="PK077_02025"
     CDS             7001..>8126
                     /locus_tag="PK077_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HRN23108.1"
                     /translation="MKAFLFSFCYNILYTVGWLVTLPSYLLKQKRRGGFGTGLLERFG
                     LYRVSYNREPKGVLYVHAVSVGEVVLALKFLRAWLRERGGSAVLATSTATGHATAVSA
                     QIPGVRVIYAPFDLLGLPGRCFDRFEPEAIVLVEAELWPNFARAAKVRGIPMAMINAR
                     MSARSESRYRAFKWISKYYFSFLDAMGVQDKGDVRRFESVGVRSSIIHVTGSIKFDQQ
                     MAERREANAEFASILDKLKRGKPVVLAASTHDREEVLIAEAARKAGGFPLIVPRHAER
                     RHAVVRELEAQGWQCVLRTDGEIPETLKDHVCYIADTTGELRDWTALADVAVIGKSFL
                     ADGGQNPAEAVACGVPVLTGPHMENFDALVQLLEGVDGIAR"
     assembly_gap    8127..8136
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8258..9118)
                     /locus_tag="PK077_02030"
     CDS             complement(8258..9118)
                     /locus_tag="PK077_02030"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="retropepsin-like aspartic protease"
                     /protein_id="HRN23109.1"
                     /translation="MSITDLKPVKLERDAHSKLLVAQCTVNGVPCNLIVDTAASHTTF
                     DIKFIKKNFPGLPLEDVQIAPGSNVRAAPRLFAMRSFSMGGLHIKNFFGLALDLTPLQ
                     KDMQVKIDGILGINYLGFSPFLLSVRNASLQFLERSRFPQHMKPLDVERDPSGIFYIR
                     CSRGGDTFPLALDSGSSLSMAPLKQWPADPNGNQLTTKATDINGHNGDCTVMKLGIPS
                     TLRMGPDFQMDGLSFAVTGTGGRYQIGVDTFQHFDIIIDAPQGEVFALPPHSRPEPRG
                     QKAPDIKQES"
     gene            complement(9280..10194)
                     /gene="rsgA"
                     /locus_tag="PK077_02035"
     CDS             complement(9280..10194)
                     /gene="rsgA"
                     /locus_tag="PK077_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRN23110.1"
                     /translation="MIRARERVRAVRDDEAVIRSILPRQTCFSRKAPGKSSAEQVLGT
                     NVDIVAVVTEPGTDFNPRRMERYFTLIRRSGASPLILLNKTDLFSREEVEKAMQILRE
                     LSPECAIISISALHNRGITEFRKCLSKGKTICIVGSSGVGKSTLVNTLLGDEWLWTGE
                     VNEVTGKGRHTTVARELVLLKHGGLLIDNPGIREIQMWTDEHTLRESFADLTELAHEC
                     KFADCSHGKDSGCAIRKAVDEGKLDKERYLNFLNLENEIARLAKRQKKRQMNVERAGK
                     RDRKVQARNYEDRVELERRHKPNHRSHD"
     gene            complement(10154..10420)
                     /locus_tag="PK077_02040"
     CDS             complement(10154..10420)
                     /locus_tag="PK077_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23111.1"
                     /translation="MPVTLQDLGWNDHFQRAFDAVAKPGWMPGRLIRETKINFTALLD
                     GGEDVDAVVSGKLWHDAATDAELPAVGDWVAIDPCTRACPCRAR"
     gene            complement(10502..11176)
                     /locus_tag="PK077_02045"
     CDS             complement(10502..11176)
                     /locus_tag="PK077_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HRN23112.1"
                     /translation="MRFLITAGPTREAIDPVRYLTNHSSGKMGYCLAEAAVHEGHSVL
                     LISGPTSLDIPHGVDFLPVECAREMYDAVKNQAPYADAAILSAAVADYRPVSVPDRKI
                     KKTGERMILELERTADILGSMRAEFGFRGILVGFAAETHDVENYARGKLERKRCDMIV
                     ANDVSRSDIGFNSSENEVLLVYPDRTELLEKAPKTQIALQIIERACRLAQGKAPSWRT
                     ADPFHA"
     gene            11247..12449
                     /locus_tag="PK077_02050"
     CDS             11247..12449
                     /locus_tag="PK077_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRN23113.1"
                     /translation="MTPSRDWGSYEGVIYWDNNATTPLAPEVYEAMEPFLKERFFNPS
                     AGYGCARHVREAVEEARAAVAALLGAFPSEIVFTSGGTEASNMAFRQMAGEREGGIGV
                     LSTDHDASLKTALALGSGCVCPVDREGRAVPEKWESMCAGGVSGVSFAWANNETGALQ
                     DAAVLCSSAGKHGVPVHLDIVQCAGKIPVDLHGMEVDYASVSAHKFHGPKGIGCLYRR
                     AGAPFSPLLFGGGQEYGLRSGTENVPGIIGFGAAARAALRHLEEDAGRLSRMRDSFEA
                     LLRAQVGGVTVHSGGTERIPNTSNLAFDGCTAEALMLLLEPAGLLCSAGSACHTLQPM
                     PSHVLTAMGLSDEAVRSSLRFSLSFMTTQEEVEQAAALVGSAVRKVRGVQSSRTGPVL
                     VYRPEWKG"
     assembly_gap    12767..12776
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<12777..14770)
                     /locus_tag="PK077_02055"
     CDS             complement(<12777..14770)
                     /locus_tag="PK077_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HRN23114.1"
                     /translation="MANGIFADNADITLGGKILSSSENSNAYGIWAENELHLKMLDGS
                     EISAIAANENSSTQAIRSKNLYLTFDGSATINGDLMADAGMELNNGGNVVVNGNIEGK
                     HLAAESTIGTVSGKMKFDSVAGLNITASVGSLEIGMSGEDSGYIKVNTVETSANISNA
                     VLVTIENANGNVSFNSVNSATVNNAVGDISATNVTNGLNVGDVGNIRVSGTNVNVLDG
                     KTVSGDIVSTTDLILSNEGSATLTGSVSAGGNRLTINSVFGINSSAATVISAGTLGGA
                     GLRGTINNHYDNIFGFSATDDSSGSLTAATALKGTHDVVSNNISGMIDGDLDPFENTY
                     ITVKYVNVGSNVGYEIEKNTYLAEAVATNEFEKALAKTYDDLEYIDGDDAANEAAVTK
                     KRNFAAMVKSGTLGAILPQSVVHSVRMNMDLADIIHLDTLNRTSAAIDGLGAKSSGTE
                     VSGGRFSGITVRNINRFATYGGDENMDGSSDYISGGLVNFEYTAGNTFLAGFGFGGFQ
                     AKSSGKGNCGKAETQSLAVNAYTDWRFFGNFDWYFGATYAFGMNKAERMNILNKSKAD
                     WNSNLIGVFTGVRYAWKPFADTGFYLKPTIGVNADFLMNPSFTEKSGEERMSAESENY
                     TSVKSLVGIEGTYAFSNGFYFTGRMFYTHEFCDDRYGVNA"
BASE COUNT         3110 a         3623 c         4410 g         3638 t
ORIGIN      
        1 ctgatggaag cttctggacg atccatattt tttctgatag aaggaatacg gaaacagagt
       61 ataaaaaata tgttcagccg gatgttttca tggagttttg tagttgcagc gtgtttggcg
      121 ggattattcc cggcacagtc ccggggcgag gaagaagctg cccaatccgg aaccatagcc
      181 gtaaaagttc ctgcctccag cctgctgatg gcccgccagg agacgggaga aacccggttg
      241 gaccggtctt tcagtaatgc ggggttgagc ataggcggca aaaaatatgc cacgggaatc
      301 ggtacgcacg ctacgtccat gattcccctg cccgttccgg aaaacccgaa agttttgagg
      361 ctggaaggtg catgcggcat tgatgacggt gcggatggag acggaagcgt ggaattccgc
      421 gtgatgagcg ggtccgaggt tttatggagt tccggcgtga tgaggagggg gatggccgcg
      481 aagaagtttt ccattccggt ggcggaaaac ggcatacggc atctttacct gatggccgac
      541 cgggtggaca ataattccta cgaccatgcg gactgggtag atctggcctg gaagacggca
      601 ggaagtgggc aggggatgaa gggagctgtg gtgaatgcct ctgaattcgg catggttccc
      661 ggcgtcagaa aagatcaggg gccggcgctg cgggctgccg tttccgctct ccggaggcag
      721 ggcgggggcg ttttgaacat ccccagaggc atttaccatt tttatccgga gggggctttg
      781 aacatgagtt tccatatttc caaccatgac cagccgctga ttcatccggt gtgtgtgcct
      841 ctcgccgatt tgcggaatgt ccgcgtggag ggcaatggtt ccctgtttct ttttcacggc
      901 aaggtggttc ccctcctggt gatggacagc gaaaatgtca gcatcaaccg tttatccgtg
      961 gattacgaac ggtcctggtg tacggaggcg cgcgtcgtga agacggatga ccggttcacg
     1021 gaagtggaaa tagacaaaaa ggcctaccct tacgaaatcc ggaacaacag gttcgttttc
     1081 caggggaaag gctgggaaga agggatggga cactgcatgg cctttgagaa agggacggga
     1141 catattatcg ccaatacatc ggacatcggc tggaacgggc atgtggagcc gttgggcggg
     1201 agccgtctcc gtttgtcctg gaacctcagg cagaagggaa tcaagccggg ggatacgctg
     1261 gttcttcgga attataaccg cccccatccg ggatgcgtgg tgtaccgggc tcggaaaaca
     1321 tcactgaatg acgtatctct gcaccagagc tcaggcatgg ccctgctggt ccagcgatcg
     1381 gaggactttc atatgaaagg cggcggagtc atggtcagga aaggcacggg gcgcgtacac
     1441 accgccggag cggacgcgac ccatttttcc aatacccggg gcggaatcgt ggtggagaaa
     1501 gccctgttcg aagggatgat ggatgatgcc atcaatgttc attccacctg cctgggcgtg
     1561 atggaagtag tggacagcca taccctgaag tgcaaataca tgcatcggca ggcggtgggg
     1621 tttgaggtgt ttcttcccgg tgaaaaaatc cgtttcatca acggtcctac gctggaaccc
     1681 ggcggaacgg ccaccgtgaa gacggcagtg aaaaagaatt ccgcggagat ggtgattacg
     1741 gtggaagagc cgctcccctc ctccgtcagg gccggggatg ccgtggagaa tgcggatttt
     1801 tacccttccg tggttttccg caacaatatc gtccgcaaca accgtgccag aggatctctt
     1861 tttacaacgc cggagagagt gctggtggag ggcaatttgt ttgaccattc ctccggctcc
     1921 gccattctat tggcagggga tgcccagggg tggtatgaaa gcggcgcctg ccatgaagtg
     1981 gtgatccgca aaaatacatt cattaacaac ctgacctcgc gttaccagtt tacgaatgcc
     2041 attatttcca tttacccgga agtcaagcag ctggacaggc agagggatta ctatcatcgc
     2101 aatgtgctga tagaaaataa tgttttcaag acgttcgatg tgccgctgct gttcgccatt
     2161 tccacggaca acctcaagtt catcaataat aaggtcattt acaatgacga gtttaaggga
     2221 tgggggcaga aacctttcca attcagaaga tgcgccaata ttctgattaa agataacaag
     2281 gtgctgcctc cccgcacgtg gacccttgag gactgcaagc tggaaaatac cccatcagat
     2341 caggtccgct ttggtggata agttttttat tttacgtaat atccgtataa acaacatttt
     2401 tttgcggatt gcatatccgg tggcaacata tttttctaag ataaaaacat caataacttt
     2461 tcaaagttgc agagataaga agaaaaaaca aatctatttt catatttatc tattgatatt
     2521 catatggata tgtgatttct tcctgtaaaa aagcaggcgg cggatatgca atccgctatg
     2581 ttgccttgca ctatgtgcag gggacaaaag agggcggcgg aaggcaatga cgggggcatc
     2641 aaatattggt ggattttacc ttttttgtct ttcttttttt ccctgaataa ccaaagtttt
     2701 tggattgatg aatgttgtac tgcattgtgc gccatgcagc agggaatgga gggatgttgg
     2761 aagaagattt gtgaaattgg cggtagcgat gctcaaatgg ccttttatta ctacctttta
     2821 tttttgtggc atcatctgac gggtgcggag tctgaatgga tgctgaggtt atttaatata
     2881 ttttgggtat ttttatcatc ctggtttttc agaaaggaac caaaggcgct ggtgattctt
     2941 ttgatttcac cgttttttgt ttattattct aacgagttgc ggccctatat gttgcagata
     3001 gcggcctctt gcgctgtctc catgttgttt tggcaggtta gccagggaga accggtaaaa
     3061 ttccatgtct tttttggttc tctgtttttt ttatgtctta caagcctgac aggggttgta
     3121 tgggcgctcg gttttgccgc ggcattcatg gtcatggcat tccggcagtt tggagggcgg
     3181 aggtttcggc gagccctgct ctggtggatt tttccatttt ccgggttggg agcatattat
     3241 ctttacactc ttttcttggg cgcccgtgcg gtttccatat cttcctcctg gatagtcaat
     3301 gcatgcgctt ccatgtacga gttatcggga ttggccggca tggggccttc ccgtctagaa
     3361 ttgaggatgt gcatgactcc tgatgcttta tggaatatga acggattggg ggcagggatg
     3421 atctccggcg ccattcttct ggccggttct gcctgcggca tcattttgtg gaataagcgg
     3481 gctgagaggc ccttggttcc tgctttgctg gtgttgatat tgtttccagg ggccgtattc
     3541 ctttatggaa cggaaatgat ggatttccgt ttttccggac ggcattgcgc gccgttattg
     3601 cctgtattgt gtctggcctg gtccctggtg gcgtcatggg attggtcgtt ccccaggatt
     3661 gcccaaacta tcctgttcct attaatgatg attgtttggg cggtgagcga tattcgtatt
     3721 cgttgcaatg atttgtatgg ccgtgaagat ttccgctctg cggtatctta ttgcaaatta
     3781 ttgcagagca gtcatgttga cattttactt ttatgcaatg gagccggaaa ggagttttat
     3841 ggatggattc ccggcccgtt aaaggacaaa tggtctgatt ataaggtgat cgtagtttcc
     3901 cggccatccg attatgctgc ttttctccag cccatagagt cttccggcaa gtatgaacgt
     3961 tccgaattat gccgggggtt tgtcgtctat cggcggggtg gaatctccca acgagagggg
     4021 tattgatgtt tttgctgttg tttcaacatt ttgagaagag acagtgtcat tataggaatg
     4081 atggatattt cttcccgcct ggtcaaaaga tgcaggaaat ttattgaacc gtaccgggtg
     4141 acggacggca ggggattcaa gttgtcccgc tatgatcccg gcgatctcgg caagctgggc
     4201 aaagacagca agaaagaggc tgtagataag ctggaaaagg gcatcgaatt gctggagcag
     4261 cttcaggaag tattgtatgc cagcgagtct catgcgctgc tggtggtgct gcaggcgatg
     4321 gacagcgcgg gaaaggatgg cattgtgaag catgtgatgg gaggcgtcaa tccgcaaggg
     4381 tgcgtagtca gcagcttcaa gcagccttcc acgctggaac ggagccatga ttttctgtgg
     4441 aggtgcgtag cgcgcctccc caggcggggg atgattggca ttttcaaccg ttcctattat
     4501 gaagaagtcc ttagcgtgcg cgtgcatccg gagtttctgg ccggagaggg atttaatccc
     4561 tcccatgcca agagtgcttt ctgggaggag aggttcagat ccatcaataa cctggaacgc
     4621 cacctgctcg ccaacaggac gagcattgtg aaaatattcc tcaacctttc cccagaggaa
     4681 cagagaaaac gccttttggc acggctggat actccggaca aaaattggaa gttttctgaa
     4741 ggggatattc atgaacgcga attctgggac cagtaccaga aagcgtatga ggagatgata
     4801 cgccatactt cttcccggga ggctccatgg tatgtggtgc cggcggacaa caaatggtat
     4861 tcccggcttg tctgcatgtg cgccattgtg gaggccctgg cggagatgag gctggaatat
     4921 ccgaaggttt ccggggaatt gaaggagttc tttcccaaat tccgggagga actggaacgc
     4981 cccctgtcca ggggcgggga ttagagcggc attcggaaac gcggagagaa aaacggcgca
     5041 tcagcgggag attgccctcc ttctgctgcg ccggcgcatt ccgtgccata gggcaggctc
     5101 cgtttccgtc agcgcctgcg ttcccaggat tgcaccttgc cgttgatgaa aagcacgtat
     5161 ccggtgttta ccggcacata ggtgacatca ttgtaataag gataataggg acggtagaag
     5221 cccggccccc agtagggacc gccccagtag gggccggccc agaaagaggg gggaggggtg
     5281 taaaccggct ggagggagga ataaatccag cgggtggcgg atttcccgtt aaggtggcct
     5341 tccgctacgg cggaggggtc cccccaggcc aggaaaacgg ccggcggaga cattccttca
     5401 gcaatttgtc cggattccac cagtgttttc tggctgggcg ggagggaatt gaaaagggcc
     5461 gggtttttct ggatgcgcga tgccggggtg gacacgcagg aagcaaggct cagagcggcg
     5521 gacagggtag tgaaaagtaa cttccaactc atgatgtaca tacggataac actcactttg
     5581 actcttgcca agggtctttt gttgagtaaa atatacgaac acgttatgaa agcgctccgt
     5641 aatctggctt taacactgat agtccctgtt ctggtttcct ccctgtccgc agcggaaaag
     5701 ccggaaatag ttgacgaata tctcccggtc ggcaaaatgg cggaagccgc cgccgtcagc
     5761 gttgtgcttg atgaaagcct gcagccgttc atggaaaaga tagacggcgt tttcgcctcc
     5821 ctgcccgaca aggacaaaaa ggagcttgtc ggccagattg tccccggcca gcccgttcct
     5881 tatgatgagc gcctgggctg gaccaaggac gaatatgcca agtacctgga gtgctggaag
     5941 ctgaagcagg tgcaggaagt ggcccctgtg gccctgggta ttttcgcctc gggggaacgg
     6001 aatatctggg atttggctgc tgtggcgcag caggggccgc tgccgatgag cacgctgaaa
     6061 tatgattcca gtaccaaatc ctggatttct cccaacggaa ccctgactct gaaaggggac
     6121 gtgtcctatg acgatctgaa tgtatacggc gcgtggagcg gcaaggaatg gacaatggaa
     6181 aagaaaacca ttctttccac cctgacggag actatcattg cgggcaagac caaggacggc
     6241 aagtatgcct atttcgttta caacatgtct gaaaagaacc cggacaacgt tgccattgcc
     6301 aaccagtcca tcgttctgcg cgttcccgtt acccgcatcg cgggtgaccc tttgctggaa
     6361 aaggccaagg ccaaggcccg ccagtaggga tatgatgttc ctttcatttc cggtaagaca
     6421 acaatgcgta tcatcagcgg aaaggccggc ggcataaccc tgtctgttcc caagggggaa
     6481 gttcgtccca cgacagaccg ggtcagggaa gccgtttttt ccatcctgaa cccgctgatg
     6541 gaccgggccg acgtgctgga tttatttgcc gggtccggcg ccttcggcct ggaggccctc
     6601 agccgtgggg cgagagacgc ccgcatggtg gatttttccc gtctctcctg cgcggcggcc
     6661 agggccaatc tcgtcaagac gggcttggaa ggcggaactg tcatccaggg agatgccgtc
     6721 cagttcgtga gaagggaact gctggccggc aggaagtacg atattatttt tgcagatcct
     6781 ccttattgca aggggccggc ggaccgcgac tttattgttg aactggcgga ggccggagtt
     6841 gccggacttt tgaagggtgg tggcgtgttt attgcggaag tgcaggaggg ctggggtacc
     6901 ggaagggaag gcgccgcgga aattgacggc ctgaatctgg tggatacccg ccggtacggg
     6961 aaaaatatgc tgctgttcta ccaattgcct gagaagtaag atgaaagcct tcctgttttc
     7021 cttctgttac aatatcctgt ataccgtcgg gtggctggta acgctgcctt cctatctgct
     7081 taagcagaag aggagaggcg gattcggcac ggggcttctg gagcgtttcg ggctgtaccg
     7141 ggtctcctat aaccgggagc cgaaaggggt gttgtatgtg catgccgtga gcgtgggtga
     7201 agtggtgctg gctctcaagt ttctccgggc gtggctccgg gaacgcggag gttccgccgt
     7261 gctggctacc agtacggcta cggggcatgc tacggcagtg agtgcgcaga ttcccggcgt
     7321 gcgcgtgatt tatgccccct ttgaccttct ggggctgccg gggaggtgtt ttgaccgttt
     7381 tgaaccggag gccattgtcc tggtggaggc cgagttgtgg ccgaattttg cccgtgcagc
     7441 caaggtgcgc ggcataccca tggcgatgat taatgcccgc atgtccgcca ggtccgagag
     7501 ccgctaccgc gcatttaaat ggatttcaaa gtattatttt tccttccttg acgcgatggg
     7561 cgtacaggac aagggggatg tccggaggtt tgaatccgtg ggcgtgcgtt cctccatcat
     7621 ccacgtgacg ggaagcatca aatttgacca gcaaatggcg gaacgcaggg aggctaatgc
     7681 ggagtttgcc tccattttgg acaaactgaa acgcggcaag cctgtagtgc tggccgccag
     7741 cacgcatgac agggaggaag ttctgatcgc agaagcggcg cgcaaggcgg gaggcttccc
     7801 cctgatcgtg cccaggcatg cggaacgccg ccatgccgtg gtgcgggaac tggaggccca
     7861 gggctggcag tgcgtgctgc ggactgatgg cgagattccg gaaaccctga aagatcatgt
     7921 ttgctacatc gcggatacga caggggaatt gagggactgg accgctctgg cggatgtagc
     7981 cgtgatcgga aaaagctttt tggcggacgg tggacagaat ccggctgaag ccgtcgcctg
     8041 tggtgtgccc gtgctgacgg ggcctcatat ggaaaatttc gatgcactgg ttcagctgct
     8101 tgaaggagtg gacggaattg ccagatnnnn nnnnnngtgc tcaaggagat gctggacaac
     8161 cccctgctgg ctcatgccca gtcttcccgc gcccaggtgg cgttgaaggc ccatttcggc
     8221 gctaccgccc ggaccattcg catgatctgc atcatgctca agattcctgt ttgatatcgg
     8281 gggctttttg cccccggggt tccggcctgg aatggggagg aagggcgaag acttccccct
     8341 gcggagcatc aatgatgatg tcaaaatgct ggaaagtatc cacgccgatc tggtatcggc
     8401 cccctgttcc cgttacggcg aaggaaaggc cgtccatttg gaagtccggt cccatgcgga
     8461 gcgtggaggg gatacccagt ttcatgacag tacagtcccc attgtggccg ttaatgtcgg
     8521 tcgccttggt ggtgagctga ttgccgttcg gatccgcggg ccactgtttc agcggggcca
     8581 tggaaagaga agagccggag tcaagagcca gcgggaaagt gtctcctccc cgggaacagc
     8641 ggatgtaaaa aattccggaa ggatcacgtt ccacatccag cggtttcatg tgttggggga
     8701 agcgggagcg ttccagaaac tgcagggaag cattcctgac ggacagcagg aacggagaga
     8761 accccaggta gttgatgccc aggatgccgt ctatcttcac ctgcatgtcc ttttgcaggg
     8821 gagtaagatc cagggcaagg ccgaagaagt ttttaatatg aagcccgccc atggagaagg
     8881 accgcatggc aaatagccgt ggtgcggcac ggacgttgga accgggggcg atttgaacgt
     8941 cttcaagcgg caggccggga aaattctttt tgatgaattt gatgtcaaat gtggtgtggg
     9001 aggccgccgt gtccacaata agattgcatg ggactccatt gacagtgcat tgcgctacaa
     9061 gaagcttgct gtgggcatcc ctctccagct ttacgggctt caggtcagta atggacacgg
     9121 cgggaccgcc ttgagcagga accggctgtt cttctgcgga taagggaagc gcccccaatg
     9181 ccagaagagc caggaaagtt cggatgaggc tgcgtgaaaa tagcattctt caccattagg
     9241 tgcgtttttg cagtttgaca agccaatttt gacggtgaat cagtcatggc tgcggtggtt
     9301 gggtttgtgg cggcgttcca gttccacgcg gtcttcataa ttccgggcct gcactttccg
     9361 gtctcttttt ccggcccgtt ccacgttcat ttgccttttt ttctgacgtt tggcaagccg
     9421 ggcgatttca ttttccaaat tcaggaagtt taagtaccgc tccttgtcca gcttcccctc
     9481 gtccacggct ttcctgatgg cgcagccgga atctttaccg tggctgcaat ccgcaaattt
     9541 gcattcatgg gccagctccg tcaaatccgc aaagctttca cggagggtat gttcatcagt
     9601 ccacatctgg atttcccgta tgccggggtt gtcaatcagc aaacctccat gcttgaggag
     9661 caccagctcc cgcgccactg tcgtatggcg ccctttgccg gttacttcat tgacttcacc
     9721 cgtccagagc cattcatccc ccagcagggt attgaccagg gtggattttc caacgcctga
     9781 ggagccgacg atgcagatgg tttttccctt ggagaggcat ttgcggaatt ccgtgattcc
     9841 cctgttgtgc agggcgctga tgctgattat ggcgcattcc ggggagagct cccgcagaat
     9901 ctgcatggct ttttccactt cctccctgga aaaaagatcc gttttgttaa gcaggatgag
     9961 aggagatgct ccgctgcggc ggatgagtgt aaagtagcgc tccatgcgcc tggggttgaa
    10021 gtccgttccc ggctccgtca ctacggccac gatatccaca ttggttccca atacctgttc
    10081 cgcgctgctt ttcccaggag ctttgcggga aaaacaggtc tggcgcggga gaatggagcg
    10141 tatgacggct tcgtcatcgc gcacggcacg gacacgctcg cgtgcacgga tcaatcgcca
    10201 cccagtctcc aacagcgggc agttccgcat ccgttgccgc atcatgccag agtttgccgc
    10261 tgacgacggc gtccacatct tccccgccgt ccagaagagc agtgaagttg atttttgttt
    10321 cccggataag acgccccggc atccagcctg gtttggcaac ggcgtcgaaa gcacgttgaa
    10381 agtggtcgtt ccagcctaaa tcctgaagag tgacgggcat gaaaatggaa aatgtagttt
    10441 tgggaaaggc cgccggcatt tacatgccga cattggatcg tggggcaaac cttcttccgg
    10501 ttcaagcgtg gaacgggtct gccgtgcgcc atgacggagc ttttccctgt gcgagacggc
    10561 atgcgcgctc aatgatttga agggcaattt gcgttttagg cgctttttcc aaaagctccg
    10621 tccggtccgg gtaaacgagc agcacttcat tttcagagga gttaaaacca atgtcactgc
    10681 gggaaacgtc gttggccaca atcatgtcgc accgcttgcg ttccagtttg ccgcgcgcgt
    10741 aattttccac gtcatgggtt tccgccgcga agcctaccag aatgcctctg aagccgaatt
    10801 ccgcgcgcat ggagccaaga atatccgcag tacgttccag ttccaaaatc atgcgctccc
    10861 ccgttttctt gattttgcgg tccggaacgg agacggggcg ataatccgct acggcggcgc
    10921 tgaggatggc ggcgtcggcg tagggagcct ggtttttgac agcatcatac atttcccgtg
    10981 cacattcaac cggcaggaag tccacgccgt gcgggatgtc cagggaagtg gggccggaga
    11041 tgagaaggac gctgtggcct tcgtgaacag ccgcttccgc caggcaatac cccatttttc
    11101 cagaggagtg gttggtgagg tatctgacgg ggtcaatagc ttccctggtg gggcctgccg
    11161 tgataagaaa acgcatgaga ggcattatgc ggaatcacgg gtaaaagtga agagtgatct
    11221 tgtgcagctg cgcattatgt tccggcttga ctccttcccg cgattggggg agttatgaag
    11281 gcgtgattta ttgggataat aacgctacca caccgctggc cccggaggtt tacgaggcca
    11341 tggagccgtt tttaaaagaa cggtttttca acccgtccgc cggttacggc tgtgccaggc
    11401 atgtgcgtga ggcggtggag gaggcccgtg cggccgttgc cgccctgttg ggcgcatttc
    11461 cttcggaaat tgtatttact tccggcggaa cggaggcgtc taatatggct ttccgccaga
    11521 tggccgggga gcgggaggga gggataggcg ttttgtccac ggaccatgac gcctccttga
    11581 agacggccct ggccctgggc agcggctgtg tctgccctgt ggaccgggaa gggcgagcgg
    11641 ttccggaaaa atgggagtcg atgtgcgcag ggggcgtatc cggcgtttct tttgcctggg
    11701 ccaataatga gacaggcgca ttgcaggatg cggcggtttt gtgttcctcc gccgggaaac
    11761 atggagttcc cgtgcatttg gatatagtgc agtgcgccgg aaaaattccc gtggatttgc
    11821 atggaatgga agtggattat gcttccgtat ccgcacacaa attccatggc cccaaaggga
    11881 ttggatgtct gtacaggaga gcgggggctc ctttttcccc tttgctgttc gggggggggc
    11941 aggagtacgg tttgaggtcc ggcacggaga atgttccggg aatcattggt tttggtgcgg
    12001 cggcgcgggc ggcattgagg cacttggagg aggacgccgg acggctgtcc cggatgcggg
    12061 attcttttga ggctctcctg cgggcacagg tgggcggcgt gacggtgcat tccggcggaa
    12121 cggaacgtat tcccaatact tccaatctgg cgtttgacgg atgtacggcg gaagcgctga
    12181 tgctgttgct ggaacctgcc ggcctgctgt gttcggctgg gtctgcctgc catacgctgc
    12241 agccgatgcc ttcccacgtg ctgacggcca tgggattgtc tgatgaggcg gtacgttcct
    12301 ccctgcgttt ttccctgtct ttcatgacga cgcaggagga ggtggaacag gctgccgccc
    12361 tggtgggctc cgccgttcgg aaggtgcgcg gcgttcagtc ttcgcgtacc ggcccggtgc
    12421 tcgtgtacag gccagaatgg aagggctgag aggctggggc gcttttacag aaagccgttt
    12481 gtgttccatg acaccggagc tcagtaacag cggttccctc cctatcaatg cttttacaga
    12541 tgtttatgag gcgattctat tgcggcagaa atatattgca gattttttgc tcattcgcga
    12601 atcgacttaa aatttaaacg tgacgcccat attgacgttg tggcgcactt tgtccgaaac
    12661 gtccgcggca tagtcggcat agacgctcca ctgttccgtg atgctgtatc cgatgcctgc
    12721 gccgaatacg ccagcgtccc tgtccatttt ccagcccctt acccttnnnn nnnnnnatgg
    12781 cgtttactcc gtagcggtcg tcgcagaatt cgtgcgtgta gaacattctg cccgtaaagt
    12841 agaagccgtt tgaaaacgcg taagtgccct cgattcccac aagcgacttt acgcttgtat
    12901 agttttctga ttccgcgctc atgcgttctt ccccgctttt ttccgtgaac gacggattca
    12961 tcaggaagtc cgcgtttacg ccgattgtgg gtttcaggta aaatccggtg tcggcaaaag
    13021 gcttccacgc gtatcgaacg cccgtgaaga caccgatgag gtttgaattc caatcggctt
    13081 tgcttttgtt caggatgttc atcctttcgg ctttgttcat gccgaaagca taggtcgcgc
    13141 cgaagtacca gtcaaagttg ccgaagaacc gccagtcggt gtaggcgttg accgcaaggc
    13201 tttgggtttc cgccttgccg cagttgccct tgcctgacga ttttgcctga aaaccgccga
    13261 agccgaagcc cgcaagaaac gtgttacccg ccgtgtattc aaaattcacc aagccgcccg
    13321 aaatatagtc gctcgaccca tccatgtttt catcgccgcc gtaggtggca aacctgttta
    13381 tgttgcggac ggtaatgcct gaaaaacgcc cccccgaaac ttccgtaccg ctgctttttg
    13441 cgccgaggcc gtcgattgcc gcgctcgtgc ggttgagcgt gtcgaggtgg attatgtcgg
    13501 caagatccat gttcatgcgc acgctgtgca ctaccgactg cggaagtatc gcacccagtg
    13561 tgccgctttt taccattgcg gcgaaattac gttttttggt aactgccgcc tcgttcgccg
    13621 cgtcgtcgcc gtcgatgtat tcaagatcgt cgtaggtttt tgccaacgcc ttttcgaatt
    13681 cgtttgtcgc aactgcttcg gcaagatagg tgttcttctc gatttcgtag ccgacattgc
    13741 tgccgacgtt gacgtacttc acggtgatgt aggtgttttc aaaaggatcc aagtcgccgt
    13801 cgatcatgcc cgaaatgttg ttcgagacga cgtcgtgcgt gcctttcaag gcggttgcgg
    13861 cggtgaggct tcccgaagag tcgtcggttg cggagaaacc gaagatgttg tcgtagtggt
    13921 tgttgatggt gccgcgcaag cccgcgcccc cgagtgtccc cgcgctgatt acggtcgcgg
    13981 ctgaagagtt tatgccgaag acgctgttta tcgtaagtct gttcccgccc gccgaaactg
    14041 aacccgtgag tgttgccgaa ccttcgtttg acagtatgag gtctgtcgtc gaaacgatgt
    14101 cgccgctcac agttttgccg tcaagcacat tgacattcgt accgcttacg cggatgttgc
    14161 cgacatcgcc gacgttcagt ccgttagtga catttgttgc tgagatgtcg ccaacggcgt
    14221 tgtttaccgt cgccgaatta accgagttga acgagacgtt gccgtttgcg ttttcgattg
    14281 tcaccaaaac ggcgtttgaa atgtttgctg atgtctcgac tgtgttgact ttaatatacc
    14341 ccgaatcctc gcccgacata ccgatttcga gcgaacccac tgatgcggta atgttcaggc
    14401 cggcaaccga gtcgaatttc attttgcctg aaaccgtgcc gattgttgat tctgcggcaa
    14461 gatgtttacc ctcgatattg ccgttgacga ctacattgcc tccgttgttt agctccatgc
    14521 cggcgtccgc catgaggtcg ccgtttatcg tcgcgctgcc gtcgaatgtc agatagaggt
    14581 ttttcgagcg gattgcctgg gtgctggagt tttcgtttgc agcgattgcc gagatttccg
    14641 aaccatcaag catttttaag tgaagctcgt tttccgccca aattccgtag gcgttggagt
    14701 tttcgcttga ggataggatt ttgccgccga gggtgatgtc agcgttgtcg gcaaaaatac
    14761 cgtttgccaa tccgttttca gacgaaaccg taatgttgcc t
//
LOCUS       DAOMSU010000024        14788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_158_length_14788_cov_2.530781, whole genome shotgun sequence.
ACCESSION   DAOMSU010000024 DAOMSU010000000
VERSION     DAOMSU010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 14788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14788
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_158_length_14788_cov_2.530781"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      complement(153..299)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            637..3213
                     /locus_tag="PK077_02060"
     CDS             637..3213
                     /locus_tag="PK077_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HRN23115.1"
                     /translation="MNVFSHGGDLKSLAEEACRPERDILDFSVNLRPEGMPEFIVSAL
                     WKAMENAVPYPSPDAADLRELAAVHYGLPSGCFVFGNGANELIHALPRALNLKQAVIP
                     EPAFSEYRLACLRHGMDVLSIRTEERNSFLPSLCHLEEQAADGSAVFLANPSNPSGSL
                     LDAAALRRAVQSRPEVLWIIDESFMDYAEGAESLLREAAPLPNLVVLRSLTKFYGMAG
                     IRCGFSICAAPLAERLRQSLPAWNVNAFAAEAAKAVLKHPSSWADRERARNRERRDDL
                     FRRLSSLPGAAVLPSEANFLLFRLAGAPHGLAARLLKKYGIALRDCSNYPGLETGGWF
                     RSGVRTPEEHALLAEALRAELAGNGPSIIRKSPRPALMIQGTCSDAGKSVLTAALCRI
                     FLQDGYRVAPFKAQNMALNSGVTALGEEMGRAQIVQAQACRIDPDARMNPILLKPHSN
                     TGSQVIVMGRPVGSMDAREYFTAKRRFWPDVCKAYDSLADEYELICLEGAGSPGEINL
                     KSADVVNMNMARYARARVLLAGDIDRGGVYASFLGTWMTFAPWEKELLAGFVVNKFRG
                     DPDLLAPAHSYMRNHTGKPVLGVIPMMRDINIPEEDRATLPPGHGEHGKHADCLDVAV
                     VMPAHVSNFTDFAPLAAEPDVRLRQVRTREEWGNPDLVILPGTKSVAADLASLRSAGL
                     EEPIRRHAEKGKWLLGVCGGLQMLGTDILDPLHMESPEERTPGLGLLELSTTFAAAKT
                     LINVRRASTPLPVPAAGYEIHHGVTIHQESTTPVMFREDGSPCGYGKGRIWATYLHGM
                     LDGDQFRRAFINMVRKDSGLKANPALHTAYDLDGALDRLADVVRKHLDLKTIYRALQQ
                     KR"
     gene            3217..4209
                     /gene="cbiB"
                     /locus_tag="PK077_02065"
     CDS             3217..4209
                     /gene="cbiB"
                     /locus_tag="PK077_02065"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HRN23116.1"
                     /translation="MFPCPFILPAAFLLDILAGEPPNRFHPVCLIGRCALRMETLARQ
                     KWGGTFHAGMAAALATCIAAWFGCAALFLPFSLLPYPWAPWIPSVLVIYICMAPRSLA
                     EHARKVENALRSGNAGEARHSVSMIVGRDTERMDVYGIARAAIESVAENLTDGVFSTL
                     FWASAGCLAGGASGAASAALAHRVFNILDAMWGKKNDRYKHFGTFAARTDDALNFIPA
                     RLILPCISFSSLMVKGTSARRALTMGWAFRNAHASPNSAWSEAAFAGALGLRLGGPVS
                     YKGIPANYPWIGTGRTKASASDLALAIRLMWTTAVTGTLAFTLLLLFTPHFWTS"
     gene            4206..5246
                     /gene="cobT"
                     /locus_tag="PK077_02070"
     CDS             4206..5246
                     /gene="cobT"
                     /locus_tag="PK077_02070"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF000996.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HRN23117.1"
                     /translation="MNKLHIPPLDEQAAKAALEHQKILAKPPLALGKLEPVAIRIAGM
                     TGNPAPRLKDKAIVLFAADHHIADHGLSLTSTDVTYIQTRNFLQGGGTINAFTRNAGA
                     RLSVVDVGVNYDFGDLPGLVKRKVMHGANDFSRGPAMTREQALKCLQVGIDMAREEKN
                     KGLDIVAAGEMGIGNTTPSSAIVAVLTGTPVETVTGRGSGVRGEVIRKKIKLIEQGIA
                     LNKPDPSDAIDVLAKVGGPEIGAMAGLMLGAASLRVPIVIDGFIAGAAAAIAQGIRPE
                     AAQYFIGSHNSAEPGHKLIMDHIGVTMYMDLGLCLGEGTGAALFFPLLDAATRVLSEM
                     KTLPELDITVPR"
     gene            5266..5550
                     /locus_tag="PK077_02075"
     CDS             5266..5550
                     /locus_tag="PK077_02075"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRN23118.1"
                     /translation="MAVITTIRSAFGFLTRLPVGPWPLQNDLNGISAWLPLVGLVVGG
                     LAGGLTWTATLLFPPLVCGIIGCACWVAVTGGLHLDGVADCGDGLPIEVS"
     gene            5547..5984
                     /locus_tag="PK077_02080"
     CDS             5547..5984
                     /locus_tag="PK077_02080"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:NF014685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HRN23119.1"
                     /translation="MKDSRLGTFGGTALFFNLAFKGAALATLAAHGSWELLLTACALA
                     GLLARSQIFIAMRFPGARPGGMGEAFKQGTRPGHALAAAAVTLAACALAGWNGLYALL
                     SALAGSMGLLLYARRRLGGVTGDVFGCTVECTEWLVLLTFCTL"
     gene            5981..6796
                     /gene="cbiR"
                     /locus_tag="PK077_02085"
     CDS             5981..6796
                     /gene="cbiR"
                     /locus_tag="PK077_02085"
                     /inference="COORDINATES: protein motif:HMM:NF041277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobamide remodeling phosphodiesterase CbiR"
                     /protein_id="HRN23120.1"
                     /translation="MKTPHKCRVPGLNIGCTSFIIPDYYVPAIRECVHYADDVALLLL
                     EAGECGKELITPAEIRELAGIAADAGVTWNVHLPTDGSFATEESSRRYTENIIRAIDL
                     TRELEPHTWVMHVVTDHIPGPDMRPHLTECETERILRSLEQITPHLPAPECLALENLE
                     RHPTDYLDKLVAATPHSRCFDIGHVWKEGLRPEKLLPLWLPDIRMCHLHGLEKRDHKS
                     LHHMPAATLDAILHPMWDLHFSPLITLEVFSLDDFLNSHQAMLESHERYITKH"
     gene            6771..7361
                     /locus_tag="PK077_02090"
     CDS             6771..7361
                     /locus_tag="PK077_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HRN23121.1"
                     /translation="MNATSPNTDANTARMTLVLGGIRSGKSQYAEQIAAGFGKKILYV
                     ATAEVWPGAGSMEYRVRKHQERRPKSWLTLECPRHVASAVGESGLLDQVDGVILECVT
                     LLASNTLYAQKDPTDYEPFQEALIEEIEALKKLIRQAPVPWVLVSSETGMGISQSDAE
                     TRHYCDGLGIANQLLAKSADEVYFMVAGLPLTVKKG"
     gene            complement(7441..10815)
                     /locus_tag="PK077_02095"
     CDS             complement(7441..10815)
                     /locus_tag="PK077_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRN23122.1"
                     /translation="MNAISIRSTKKVEPKCYAYTTPEVQRHEGWTKIGYTEQDDVEVR
                     IRQQTQTVDVRYCLEWHESARFADGTVFHDKAFHRYLGKKEVEREGNSEWFHLVPEVA
                     HGHFREFTRNRGVLQTPGVVPYVLRAEQAEAVERTRLYMASHAGQSPEFLWNAKPRFG
                     KTLAAYDLCKQMGARTVLIVTNRPAIAHSWYDDYVRFMGTESGYYFISGADALRGLPH
                     VLERAQLPSDAEGYIEFVSLQDLKGSLYFGGEFDKLEHVQKLTWDLLIVDEAHEGVDT
                     YRADVAFEQIARRATLHLSGTPFKALANEKFEEKAIFNWTYADEQCAKREWQEEAEEE
                     NPYAALPQLHLYTYRMSEVVRDRLKQGADFDDDGENEAYAFDLNEFFATKSDGSFKYD
                     EAVERFLEALTGQEKFPFSTEALRREVKHSFWLLDRVDSAKALAKKLKAHPVFREYEV
                     VVAAGDGKTDAEEEATSTLKSLDKVRTAIRAHERTITLSVGQLTTGVTVPEWTAVLML
                     SNVKSPSLYMQAAFRAQNPCLFVEGDELRRKENAYVFDFDPARTLVIFEQFANDLCSA
                     TVSGGGDGETRKRQVCELLNFFPVYGEDEEGEMVALDAEKVLSVPRKIHAVEVVRRGF
                     MSNFLFQNIGNIFSAPRQVADILGQLPTVKEQKPSKAKVGGQGEGGLLDGAADLPLNE
                     KGEVVLPLDELTRKTGELFGDKIYSDNVVTQTKIDEVSTSNAPDKKAAQDLVTILVDT
                     VVNPTLQDAKTHYGDEMSRASEHRLKKQLQNQATVAVGKAATTHKKNRHETEKAKKRA
                     LDEAQAKGQSEAEVQAIQDRFDAEQRENERRYREELKEAAAEIEKKAKQAVVDTVETE
                     KVTRKKEAIEEDIRGHLRGFARTIPAFLMAYGDENTCLDNFDTILPDEVFREVTGISL
                     EQFRILRDGCDYEDEGVRKHFDGHCFDPIVFDDAVQEFLALKKKLGNYFDESQSEDIF
                     DYIPPQKTNQIFTPKHLVMKMVDMLEEESPGCFDDPDKTFIDLYMKSGLYVAEIVKRL
                     FRSEAMKARFPQAEERLRASGVWLGADGDYLPHCLCLCPGLRRGAAHRAASSRALRCL
                     AGGQRRHAGGGSGQDLGEVTVMTAWLGRLHVLRRSR"
     gene            complement(10821..12782)
                     /locus_tag="PK077_02100"
     CDS             complement(10821..12782)
                     /locus_tag="PK077_02100"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HRN23123.1"
                     /translation="MKHRGNEASGETQLDASLVAILLADRSSGAFIRWACNAYTTHGE
                     SYAADQEIYPHQVHLIQERTRKTQEEQRDRTKKSAEVFTPAWLCNAMINARDAVYFGR
                     EEVFNRMEAPSWTPTRKTIDFPTTASGRRLAWERYIDARCLEITCGEAPFLVSRYDAA
                     DGRPIPLAERIGILDRKLRIIGEHTCTAEDWFHWAKRALESAYAYEYQGDSLFLARLN
                     LFLSISEYHRHLWKRPLNRHQQEEVARILSWNLWQMDGLTATTPFVTKQGKPEDSLFD
                     FYAITAERRPLRSLIRDWRGKKTIRFSELNLSTTMKFDFVIGNPPYQEMSKNNKMTRS
                     IYPDFVRSSQAIGQIVSLVMPARWMSGENGPYRETQHFIDEMLKGDHLQSFHLYPNSS
                     DLFPNVDIKGGVCFFVWNRAYSCDRVNYTITENGAYKKVLTSFRIAENVIIRFPELIS
                     ILEKTKAISTIYLNTWVSSRNPFGFISDLFTKNKEGVSRISEEKQQDDYLVHGLLKNK
                     RVIRYIPNSALKKNIHIAHSYKVFLPRANGSGVFGEVFSTPMIGTPMMICTETFLQIG
                     VFDNCTEAENLLKYVKTKYFRTMVGIKKTAVFNYKECFTFVPLQDFTAASDIDWSRSV
                     AEIDRQLYAKYGLSAEEVAFIEEKIKPME"
     gene            12935..14026
                     /locus_tag="PK077_02105"
     CDS             12935..14026
                     /locus_tag="PK077_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRN23124.1"
                     /translation="MFLTSFVILGLMKSIIYPVAMNTDASTALALLASLPFSLTDVAR
                     LMLELVEGSGGSSVRKKEALLLHCRRVIALGCEAEHLATQTVSFSKAVAETLRVKADR
                     SALTLRDIRSFTQRMMRDVPDLAARPMRSMTTADCAAVLEKVFLSPSQRRHARAILSG
                     VFTVAWKKGWCAHNPVRLVDVPRVTEREIVPLRIEEVRRLLRTAEREEFSPCAAGVAM
                     MLYGGIRPYEVRRLTWGDVDWEEGEVRIRPRQSKTGGGRQVPLSRSVLAWLRSYYPQG
                     AERESVFICPPDWNRRWRALRSAAGFQTWRQDVLRHTFASYHAKMFHDWGRLQAAMGH
                     RDGTLLQTRYVHTQGIRGCEVRAFWELAA"
     gene            14210..14292
                     /locus_tag="PK077_02110"
     tRNA            14210..14292
                     /locus_tag="PK077_02110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14243..14245,aa:Leu,seq:caa)
     gene            complement(14552..>14788)
                     /locus_tag="PK077_02115"
     CDS             complement(14552..>14788)
                     /locus_tag="PK077_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23125.1"
                     /translation="AACTIHALLSDASSLVILETENPGQYFFIAPTDTIEVSDEHALV
                     TQTFKAAAPGLSARSGIRPGVNADLKNPTVEKAV"
BASE COUNT         3178 a         4554 c         3907 g         3149 t
ORIGIN      
        1 tgacggaggg cttggagtct tcctttttag cggactcggc aagagaggac tggcctgcga
       61 atgcggcaag gccaacagca attgcgccca tttgaagggc tttcttcgta taaagcatgg
      121 ttgtagtgcc ttacgttcgg taactcgccg aacagttgga caacctcaca ggctggtttt
      181 ctggcttgcg aggcaggctc gcttacagtg gcggtaccgc tccggactca caccggtatt
      241 ccccatttat ttcctgcccg gctacgggcg tccctgaggt ctgtaaccct tcccataagg
      301 gttcatggga cggataaaat accaaagaga ccggtatgac aagacaaaaa aatttttctt
      361 tgaacatcca gatgaattgc agtcgttttc ccttttcagc gtattgttct ccatccctct
      421 tttcctcacg gggctttcca tttttgtctt gtcactcccg gcgcatacag gacatgatgc
      481 gctcatgacg ctccttgttc ccacgttccc gcaacaggga agatttcccg tctccggcga
      541 agcttccggc tacgaggaat tccgaacttg caaggcgctt cctttctttc tccggcgcgg
      601 acaacccttc ggtacgccgg ttccaattac tgtggcatga acgttttttc acatggcgga
      661 gatttaaaat ccctggcgga ggaggcctgc cgcccggaac gggatatttt ggacttcagc
      721 gtcaacctga ggccggaagg tatgccggag ttcatcgttt cagcattgtg gaaggccatg
      781 gaaaacgcag taccctaccc ttctccggat gcggcggatc tgcgagaact ggcggcggtt
      841 cattatgggc ttccttccgg ctgcttcgta tttggaaacg gggccaatga actcattcac
      901 gctcttccgc gcgcattaaa ccttaaacag gccgtcattc cggaacccgc tttttccgaa
      961 tacaggctgg cctgcctgcg ccacggcatg gatgttcttt ccatccggac ggaggaacgg
     1021 aattcctttc tcccgtctct ttgccatctg gaggaacagg ccgcagacgg aagcgctgtt
     1081 tttctggcaa atcccagcaa tccgtccggc agcctgctgg acgcagcggc cctgcgcagg
     1141 gctgtccaga gccgccccga agtcctctgg atcattgacg aatctttcat ggattacgca
     1201 gaaggagcgg aatcgctgct ccgagaagcg gccccccttc ccaacctggt ggttctgcgc
     1261 tccctgacca agttttacgg catggccggc atccggtgcg gtttttccat ctgtgccgct
     1321 ccgctcgcgg agcggctgcg gcaatccctg cccgcctgga acgtgaatgc ctttgcggcg
     1381 gaagcggcga aggccgtgct gaaacacccc tcttcctggg cagacaggga acgcgcccgg
     1441 aaccgggaac gccgggatga cctgttccgc aggctttcct ccctgccggg tgccgccgta
     1501 ctcccgtccg aggccaactt cctgctcttc cgcctggcgg gggcgcctca tggcctggca
     1561 gcccggctcc tgaaaaaata cggcatcgcg ctgcgcgact gttccaatta tccgggactg
     1621 gaaacgggcg gctggttccg ctccggcgtc cgcacgccgg aggaacacgc cctgctggca
     1681 gaagctctgc gcgccgaact ggcgggaaac ggcccttcca ttatccgtaa atcgcccaga
     1741 ccggccctga tgattcaggg cacctgctcc gatgcgggaa aaagcgtgct cacggcagcc
     1801 ctgtgccgca ttttccttca ggacggctac cgggtggccc cgttcaaggc acagaatatg
     1861 gctctcaact ccggcgtaac cgctctggga gaggaaatgg gccgcgccca gatagtgcag
     1921 gcccaggcct gccgcattga tccggatgcc agaatgaacc ccattcttct caagccccat
     1981 tccaataccg gctcccaggt gatcgtgatg gggcggcccg taggcagcat ggacgcgcgg
     2041 gaatacttca cggctaaaag gcgcttctgg ccggacgtat gcaaagcata cgattccctg
     2101 gcggatgaat atgaactcat ctgcctggaa ggggccggaa gccccggaga aatcaatcta
     2161 aaatcagcag acgtggtcaa catgaacatg gcccgctacg cgcgtgccag ggttctgctc
     2221 gccggggaca ttgaccgtgg cggagtgtac gcctcctttc tgggaacgtg gatgacattc
     2281 gccccgtggg aaaaagaact gctggcgggg ttcgtggtca acaaatttcg aggagatccg
     2341 gatctgctgg ccccggcgca cagctacatg cggaaccata cgggcaagcc tgtgctgggc
     2401 gtcatcccga tgatgcggga catcaacatt ccggaagaag accgcgccac gctgccccct
     2461 ggccacgggg agcacgggaa acatgcggat tgcctggatg tggccgtagt catgcccgcc
     2521 cacgtctcca acttcacgga cttcgcccct ctggcggcgg agccggacgt ccggctccgc
     2581 caggtgcgga cacgggagga atggggaaat ccggacctgg tcattctgcc cggcactaaa
     2641 agcgtggctg cggacctggc ctctctccgt tccgccgggc tggaagaacc catccgccgt
     2701 catgccgaaa agggaaagtg gcttctgggc gtctgcggag ggctgcaaat gctgggaacg
     2761 gacattctgg acccgctgca catggaatcc ccggaggagc gcacgccggg gctggggctg
     2821 ctggaacttt ccaccacctt cgccgccgcc aaaaccctga tcaacgtgcg ccgggcaagc
     2881 acgccgctgc ccgttcccgc cgcaggttat gaaatccacc atggcgtcac catccatcaa
     2941 gaatccacca cccccgtcat gttccgggaa gacggctccc cctgcggcta cggcaaaggc
     3001 cgtatctggg ccacgtatct gcacggcatg ctggacggag accaattccg ccgcgcattc
     3061 atcaacatgg tcaggaaaga ttcagggctg aaagccaacc cagccctgca caccgcctat
     3121 gacctggacg gagcgctgga ccgcctggcg gacgtggtcc ggaaacatct ggacctgaaa
     3181 accatttacc gagctctcca acagaaacgc tgacccatgt tcccgtgccc gttcatcctg
     3241 cctgcggcct tcctgctgga tattctggcg ggagaaccgc ccaacaggtt ccatcccgtc
     3301 tgtttgatcg gccggtgcgc gctccgcatg gaaacactgg cgaggcaaaa gtggggagga
     3361 acttttcatg cgggaatggc ggcagcgctg gcaacctgca ttgccgcatg gttcggctgc
     3421 gcagcgctct tccttccctt ttccttgtta ccctaccctt gggccccctg gattccgtcc
     3481 gttctggtca tttacatctg catggccccg cgcagcctgg ccgagcatgc ccggaaggtg
     3541 gaaaatgccc tgcgcagcgg gaacgccgga gaggcccggc actccgtctc catgatcgtg
     3601 ggacgggata cggaacggat ggacgtctac ggcatcgcca gagcggccat tgaaagcgtg
     3661 gcggagaact tgacggatgg cgttttttcc accctgttct gggcctcagc aggctgcctg
     3721 gcaggaggcg cctccggggc ggcttccgcc gcgctggcgc atcgtgtttt caacatcctg
     3781 gatgccatgt gggggaaaaa aaatgaccgg tacaaacact tcggcacctt tgccgcacgc
     3841 acggatgacg cgctcaactt catccccgca cgccttattt taccgtgtat ttccttttcc
     3901 tccctgatgg tgaaaggaac ctccgcccgg agggcgctca ccatgggatg ggccttccgg
     3961 aatgcccacg ccagccccaa ttccgcatgg agcgaagcgg ccttcgctgg agcgctgggc
     4021 ctccgtctcg ggggcccggt ctcttacaag ggaattcctg caaactaccc ctggatagga
     4081 acagggcgaa ccaaagcctc cgccagcgac ctggccctgg ccattcggct catgtggacg
     4141 actgccgtca cgggtacgct ggcgttcact ctccttcttc tcttcacccc ccacttctgg
     4201 acatcatgaa caaactgcat atccccccgt tggacgaaca ggccgcaaaa gcggccctgg
     4261 aacatcagaa aattctggcc aagcctcccc ttgccctggg aaaactggaa cccgtggcca
     4321 tccgtattgc cggcatgacc ggcaatcccg cgccgcgcct gaaggacaaa gccatagtcc
     4381 tgttcgccgc ggaccaccac attgccgacc acggcctcag cctgacatcc acggacgtca
     4441 cctacatcca gacccgcaac ttccttcagg gcggaggcac catcaacgcc tttacacgca
     4501 acgcgggagc acgcctttcc gtagtggacg taggcgtcaa ttacgacttc ggcgacctgc
     4561 cggggctggt caaaagaaaa gtcatgcacg gcgccaacga cttcagcagg gggccggcca
     4621 tgacgcggga acaggccctg aaatgcctgc aagtgggcat tgacatggcc cgtgaagaaa
     4681 aaaacaaagg gctggacatt gtggccgccg gagaaatggg cattggcaac accaccccct
     4741 cttccgccat cgtggccgtg ctcacaggca cccctgtgga aaccgtgacg ggacgcggtt
     4801 ccggcgtcag aggagaagtc atccgcaaaa aaataaaact catcgaacag ggaatcgccc
     4861 tgaacaagcc agacccttcc gatgccattg acgtgctggc gaaggtggga ggaccggaaa
     4921 taggagccat ggccggactg atgctggggg cggcctccct gcgcgtcccc atcgtcattg
     4981 acggcttcat tgccggggcg gccgcggcca tcgcccaggg cattcgcccg gaagcggcgc
     5041 aatacttcat cggctcccac aactccgcgg aaccggggca taagctcatt atggaccaca
     5101 tcggcgtcac catgtacatg gacctgggcc tctgcctggg ggaaggcacg ggggcggccc
     5161 tgttctttcc cctgctggac gccgccacgc gcgtactttc tgaaatgaaa accctgccgg
     5221 aactggacat caccgttccg cgctgaccct ttcttccctg tatccatggc tgtcatcacc
     5281 accatccgct ccgccttcgg cttcctgacc cggctacccg tagggccgtg gccgctgcaa
     5341 aatgacctga acggaatttc cgcctggcta ccgctggttg gcctggtggt gggggggctt
     5401 gcgggcggcc tgacatggac ggcaaccctg ctcttccccc ccctggtctg cggcattatc
     5461 ggctgtgcct gctgggtagc cgtcacgggc ggcctgcacc tggacggagt agcggactgc
     5521 ggagacgggc ttcccattga agtatcatga aagactcccg cctggggacc ttcggcggca
     5581 cggccctgtt tttcaacctg gcattcaagg gagccgccct ggcgactctg gcggcccacg
     5641 gctcctggga acttctgctc acggcgtgcg ctctggcagg attgcttgcc cgcagccaga
     5701 tattcatcgc catgcgcttt ccgggcgcac ggcctggagg catgggggaa gcgttcaagc
     5761 agggaacgcg ccccgggcac gccctggctg ccgcagccgt caccctggct gcctgcgctc
     5821 tggcaggatg gaacgggctg tacgccctgc tctccgccct ggccggctcc atgggcctgc
     5881 ttctatacgc gcgccgccgc ctgggcggcg ttaccgggga tgtattcggc tgtaccgtag
     5941 aatgtacgga atggcttgtc ctgctcacct tctgcacttt atgaaaactc cacacaaatg
     6001 ccgcgttcca ggcctcaaca tcggatgcac ctccttcatc atcccggact actacgtgcc
     6061 cgccatcagg gaatgcgtcc attacgcgga tgatgtcgct ctgctcctgc tggaagccgg
     6121 agaatgcgga aaggaactca ttacccctgc ggaaatacgg gaactggccg gaatcgctgc
     6181 agatgccggg gttacctgga acgtccacct gcctacggac ggcagcttcg ccacggaaga
     6241 atcaagccgc cgttatacgg aaaacatcat tcgcgccatt gacctgacac gggaactgga
     6301 gccccacacc tgggtcatgc atgtggtcac ggaccatatc cccggtcctg atatgcgccc
     6361 ccatcttacg gaatgcgaaa cggaacgaat cctccggagt ctggaacaaa tcacacccca
     6421 cctccccgca ccggaatgcc tggccctgga aaatttggaa cgccatccga ccgactatct
     6481 ggacaaactg gtagccgcta caccccattc acgttgcttt gacataggcc atgtctggaa
     6541 agaaggcctg cggccggaaa aacttctgcc gctctggctg ccggacatac gcatgtgcca
     6601 cctgcacggg ctggaaaaac gggaccacaa atccctgcac cacatgcctg ccgctacgct
     6661 ggacgccatt ctgcacccca tgtgggacct tcatttctcc ccccttatta cgctggaagt
     6721 cttcagcctg gatgacttcc tgaactccca ccaggccatg ctggaatccc atgaacgcta
     6781 catcaccaaa cactgacgcc aataccgcac gcatgacgct cgtcctgggc ggcatcagaa
     6841 gcggcaaaag ccaatacgct gaacaaatag ccgccggatt cgggaaaaaa atcctgtacg
     6901 tcgccacggc ggaagtctgg cccggagccg gctccatgga ataccgcgtg cgcaagcacc
     6961 aggaacgccg tcctaaaagc tggctcacgc tggaatgtcc ccgtcatgta gcttctgccg
     7021 tcggggaatc cggcttgctg gaccaggtgg acggcgtcat tctggaatgc gtcaccctgc
     7081 tcgcctccaa taccctgtac gcccagaagg accccacgga ctacgagccg ttccaggaag
     7141 cgctgattga ggaaatagaa gctttgaaaa agctcatccg ccaagccccc gtaccatggg
     7201 tgcttgtttc ttctgaaacc ggcatgggca tcagccagtc agatgcggaa acgcgccact
     7261 actgcgacgg gctgggcatt gccaaccaac tccttgccaa aagcgcggat gaagtctact
     7321 tcatggtggc gggactaccc ctgaccgtga aaaaaggctg aaacgccaac catgaatttt
     7381 catcggcttg cgcacatgaa acacaaagaa acatgccgga aattaagccg cgccactcat
     7441 tcaacgggag cggcgcaaga catgaagcct cccaagccag gccgtcatca cggtcacctc
     7501 tcccaaatct tgtccagagc ctcctccagc gtgccgtctt tgaccgccgg caaggcatcg
     7561 aagcgcgcga gatgatgccg ctcgatgcgc agctcctcgt cgaagcccag gacaaaggca
     7621 caggcaatgc ggtaaataat ctccgtcggc gccaagccat acacctgatg cgcgtaaacg
     7681 ctcctccgcc tgcgggaaac gcgccttcat cgcctcgctg cggaaaaggc gtttgacaat
     7741 ctccgccaca tatagacccg acttcatgta gaggtcgata aacgtcttgt cggggtcatc
     7801 gaaacagccc ggagactcct cctccaacat atcgaccatc ttcatgacaa gatgcttggg
     7861 cgtgaaaatc tgattcgtct tttgcggcgg aatataatcg aaaatatcct ccgactgcga
     7921 ctcatcaaaa taattgccta atttcttctt caacgccaag aactcctgaa cggcatcatc
     7981 aaaaacaatc ggatcaaaac aatgaccgtc aaaatgcttc cggacaccct catcttcgta
     8041 atcgcaccca tcgcgcaaga tgcggaactg ctctaaagaa atccccgtca cctctctaaa
     8101 gacctcatcg ggaagaatcg tatcgaaatt atccagacag gtattctcgt cgccatacgc
     8161 catcaaaaag gcggggatcg tccgcgcaaa gccacgcaaa tgaccgcgaa tatcctcttc
     8221 aatcgcctcc ttcttcctgg taaccttctc ggtttccacc gtatccacca cagcctgttt
     8281 ggccttcttc tcaatctctg ccgctgcctc cttcaactcc tctcgatacc gacgctcatt
     8341 ctccctctgc tcggcatcaa aacgatcctg aatcgcctga acctcggcct cactctgccc
     8401 cttggcttgc gcctcgtcga gagccctttt ctttgccttc tccgtctcgt ggcgattctt
     8461 cttgtgcgtc gtcgctgcct tgccgaccgc gaccgtcgcc tgattctgca actgcttttt
     8521 gagacgatgt tcagacgctc tactcatctc atcgccatag tgcgtcttgg catcctgcag
     8581 ggtggggttc accacagtat ccaccaatat cgtcactaaa tcttgtgcgg ccttcttatc
     8641 cggcgcgtta ctcgtcgaga cctcatcgat cttcgtttgc gtcacaacat tgtcgctgta
     8701 tatcttatcc ccgaaaagct cccctgtctt acgggtcaat tcatccaagg gcaacaccac
     8761 ttcacccttt tcattcaggg gcaaatccgc ggcgccgtcc aacaaaccgc cctctccctg
     8821 accgcccacc ttcgccttgg acggcttctg ctccttcacc gtcggcagtt gcccgaggat
     8881 atccgccacc tgacgcggcg ccgagaaaat attcccgata ttctggaaca ggaaattgct
     8941 catgaaccca cggcgcacca cctcgacggc atgaatcttg cgcggcacgg acaacacctt
     9001 ctccgcatcc agagccacca tctccccctc ctcatcctcc ccatagacgg ggaaaaaatt
     9061 cagcaactcg cacacctgcc ttttgcgcgt ctccccatcg cctccgccac tcaccgtcgc
     9121 cgaacacagg tcattggcga actgctcaaa aatcaccagc gtccgcgccg ggtcaaaatc
     9181 aaacacatac gcattctcct tgcgccgcaa ctcgtccccc tccacgaaca ggcagggatt
     9241 ctgcgcccgg aacgccgcct gcatatacag cgacggactc ttcacattgc tcagcatcaa
     9301 gaccgccgtc cactccggca ccgtcacccc cgtcgtcaac tgcccgacgg aaagcgtaat
     9361 cgtcctctcg tgggcgcgaa tcgccgtgcg caccttatcc aaggacttga gcgtcgatgt
     9421 cgcctcctcc tccgcatccg tcttgccatc gccagccgcc accaccacct cgtactcgcg
     9481 gaagacggga tgcgccttca acttcttcgc caacgccttg gcgctgtcca cgcggtccaa
     9541 cagccagaaa ctatgcttca cctcgcggcg caaagcctcc gtcgaaaagg gaaacttctc
     9601 ctgccctgtc aaagcctcca aaaacctctc caccgcctca tcgtacttaa acgaaccgtc
     9661 actcttcgtg gcaaaaaact cattcaagtc aaaagcatag gcctcatttt cgccgtcatc
     9721 gtcaaaatca gccccctgct tcaggcgatc gcgcactacc tcagacatcc ggtacgtata
     9781 aaggtgcaac tgcggcaagg cggcataggg attttcctcc tccgcctcct cctgccactc
     9841 cctcttggcg cactgttcat cggcataggt ccagttgaaa atcgccttct cctcgaactt
     9901 ttcatttgcc aaagccttaa aaggcgtccc cgacagatgc agcgtcgccc gacgcgcaat
     9961 ctgctcgaac gccacatccg ccctgtacgt atccaccccc tcgtgcgcct catccacaat
    10021 cagcagatcc caggtcaact tttgcacatg ctccaactta tcgaactccc cgccgaaata
    10081 cagagaaccc ttcaaatcct gcagagacac aaactcgata tacccctcgg catcactcgg
    10141 caactgcgcg cgctccagca catggggcaa gccccgcaga gcatccgccc cactgatgaa
    10201 ataatacccc gactccgtcc ccataaaacg cacataatca tcgtaccacg aatgagcaat
    10261 cgccggacga ttcgtcacaa tcaacaccgt ccgcgcaccc atctgcttgc acaaatcata
    10321 cgccgccaaa gtcttgccga aacgcggctt ggcattccac aaaaactccg ggctttgccc
    10381 cgcgtggctc gccatataga gccgcgtccg ctcgaccgcc tccgcctgct ccgcccgcaa
    10441 cacataaggc accacacccg gcgtctgcaa caccccgcga ttgcgggtaa actcccgaaa
    10501 atgaccatgc gccacctcgg gcacaagatg aaaccactcc gaattcccct cgcgttccac
    10561 ctccttcttg cccaagtaac gatgaaacgc cttatcgtga aacaccgtcc cgtcggcaaa
    10621 acgcgccgac tcgtgccatt ccaggcaata gcgcacatcc accgtctgcg tctgctggcg
    10681 gatgcgcacc tcgacatcat cctgctcggt gtagccaatc ttcgtccagc cctcgtgtcg
    10741 ctgcacctct ggagtcgtgt aggcgtaaca cttcggctca accttcttcg tgcttcggat
    10801 agagatggcg ttcatagggc ttattccatg ggtttgattt tttcttcaat aaaggcaacc
    10861 tcctctgcgg acaagccgta cttggcgtac agctgtcggt caatctcggc tacagaccgt
    10921 gaccagtcta tatcgctggc ggcggtgaag tcttggaggg ggacaaaagt gaaacactcc
    10981 ttgtaattaa agacagccgt tttcttaatc ccgaccattg tcctaaaata cttggttttc
    11041 acatacttca atagattttc agcttctgta cagttatcga agaccccaat ctgtaaaaat
    11101 gtttccgtgc atatcatcat aggggtacca atcatagggg tactaaagac ttcgccaaat
    11161 actccactac catttgcacg aggtagaaaa accttataag aatgggcgat gtgtatgttt
    11221 ttctttaagg cagagttggg tatatacctt atgactctct tgtttttgag aagaccgtga
    11281 accaaatagt catcttgttg cttttcttca ctaatgcgag aaacgccttc cttgttcttc
    11341 gtaaagagat cggatatgaa tccaaatgga ttcctcgatg aaacccatgt attaaggtag
    11401 attgtcgata tagctttcgt cttttccaaa atagatatga gctccggaaa gcgtataatg
    11461 acattctcgg caatgcggaa tgaagtgaga actttcttat atgcgccgtt ctctgttatc
    11521 gtataattga ccctatcgca cgaatacgct ctattccaaa caaagaaaca aactcccccc
    11581 ttgatatcaa cattggggaa caaatcagag gagtttgggt ataaatgaaa ggattgaaga
    11641 tgatccccct tcaacatttc atcaatgaaa tgttgagttt cgcgataggg cccattttca
    11701 ccggacatcc aacgagcagg catcacaagg gaaacaattt gacctattgc ttgagatgat
    11761 cttacaaaat caggataaat gctgcgagtc atcttattat ttttactcat ttcctgatac
    11821 ggcggattac cgataacaaa atcaaatttc atggtggtgg ataggttgag ttcagagaat
    11881 cgaatcgttt ttttgccgcg ccagtcgcgg atgaggctac ggagggggcg cctttcggct
    11941 gtgatggcgt aaaaatcaaa taaggaatcc tcgggcttcc cctgtttcgt gacaaaggga
    12001 gtcgtcgccg tcaggccgtc catctgccag agattccacg agaggatgcg ggcgacttcc
    12061 tcttgttggt gtcggttgag ggggcgtttc cacaggtgac ggtggtactc gctaatgctc
    12121 agaaaaagat tgagacgggc gagaaagagg ctgtcgccct ggtattcgta agcgtaggcg
    12181 ctttcgaggg cgcgttttgc ccagtggaac cagtcctctg cggtgcaggt atgctcgccg
    12241 atgatacgca gcttgcggtc gaggatgccg atgcgctccg ccaaggggat ggggcgaccg
    12301 tcggcggcat cgtagcgcga gacgagaaag ggggcttcgc cgcaggtgat ttccagacag
    12361 cgggcatcga tgtaacgctc ccacgcgaga cggcggccag atgctgtcgt cggaaagtca
    12421 atcgtcttgc gcgtcggcgt ccacgatgga gcctccatgc ggttaaagac ctcctcccgc
    12481 ccgaagtaaa cggcatcgcg ggcgttgatc atagcgttgc acagccaggc aggggtaaag
    12541 acttcggcgg attttttggt gcggtcgcgc tgttcttcct gcgtcttgcg ggtgcgctcc
    12601 tgaatcagat gcacctgatg ggggtagatc tcttgatccg ccgcatagga ctcgccgtgg
    12661 gtagtgtagg cgttgcaggc ccagcggatg aaagcgccgc tcgaacggtc ggcgaggaga
    12721 atagccacaa gggaggcatc gagttgcgtc tctcccgaag cctcattccc tcgatgtttc
    12781 atcgagggaa gtagagtata aataatgctc ttaacttcaa tgttttgatt ctcaatatat
    12841 tatattattg atccagacaa agaaacaaga aaaaaaggaa gaaaattctt ctcatttttt
    12901 tcttgtcata caggcataac taccgttcta ttccatgttt ttgacaagtt tcgttatttt
    12961 gggcttgatg aagagtatta tttatcctgt tgccatgaac accgacgcta gcaccgccct
    13021 tgccctcctc gcctctctcc ccttctccct caccgatgtt gctcgtctca tgctggagct
    13081 ggtcgaagga agcggaggct cttccgtaag aaagaaagaa gctctgctcc ttcactgccg
    13141 ccgcgtcatc gccttggggt gcgaagccga gcatctcgcc acgcaaacgg tctccttctc
    13201 caaagccgtg gcagaaaccc tgcgcgtcaa ggccgaccgt tcggcgctca ccctgcgcga
    13261 tatccgctcc ttcacgcaga ggatgatgcg agatgtgccc gatctggcgg cgcgtcccat
    13321 gcgctcgatg acgacggcgg actgtgcggc tgtgttggag aaagtctttc tttcaccctc
    13381 gcaacgtcgt catgcgcggg cgattctctc gggagtgttc acggtggcgt ggaagaaagg
    13441 ctggtgtgcg cataatcccg tgcggctggt ggatgtgccg cgcgtgacgg agcgggagat
    13501 cgtccccctc cgtatcgagg aagtgcgccg cctactgcgg acggcagaga gggaggagtt
    13561 ttccccctgt gcggcgggcg tggcgatgat gctctacggc gggattcgtc cgtatgaggt
    13621 gcggcgtttg acctggggcg atgtggattg ggaggagggg gaggtgcgga ttcgcccgag
    13681 gcagagcaag acgggcggcg gtcggcaggt gccgctgtct cgctccgtgt tggcatggtt
    13741 gaggagttat tatccgcagg gggcagagag ggagtcggtt ttcatctgtc cgccagattg
    13801 gaacaggcgg tggcgcgctt tgcgctcggc agcgggtttt cagacatggc gtcaggacgt
    13861 cctgcggcat acctttgcgt cgtatcatgc gaagatgttt cacgattggg ggcgtttgca
    13921 ggcagcgatg gggcatcggg acgggacgct gttgcagacg cgctatgtgc atacgcaggg
    13981 gattcgagga tgcgaggtgc gagcattttg ggagttggcg gcgtgagaag ggacgcaaaa
    14041 aagcgttaac gcggccgtgc actgctcatt ccaaacactt atttccatcc ggaaacatga
    14101 cggagggaat ccgcaggaaa atccctccgt tttttcagca gctcaccata ttccacagga
    14161 aattcttgac tctccagccc tcttgcctct taattctttc gcttcacgtg ccgtcgtggc
    14221 ggaatggtag acgcgctcga ctcaaaatcg agttccaaaa gagtgtgggt tcgagtccca
    14281 ccggcggtac caatcaataa accctgggag ctaaacgctc ccagggtttt ctattatcag
    14341 tcgataacgc ttcatttctc aaagagactt ccccgtagta atttcaggaa ggaatgagac
    14401 ggacttccgc acccagtccg cgtcagacaa tgatctggcg gtgaagtccc gctccccgta
    14461 aaggttcgaa aaaccatgga caaacaacct tttgaccgga ttgctcatga gcgctgcgct
    14521 taatcgcggg cgatcagaaa aaagaaatgg ttcataccgc tttttccacc gtcggatttt
    14581 ttaaatcagc atttactccc ggccttatgc cgctgcgggc agacaatccc ggagcggctg
    14641 ctttaaaagt ttgtgtcacc agggcgtgtt cgtctgatac ctctattgta tcagtgggag
    14701 ctataaaaaa atattgtccg gggttttccg tttccaggat aaccagagaa gaagcgtcgc
    14761 taagaagcgc atggatagta cacgcagc
//
LOCUS       DAOMSU010000025        14433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_166_length_14433_cov_2.677285, whole genome shotgun sequence.
ACCESSION   DAOMSU010000025 DAOMSU010000000
VERSION     DAOMSU010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 14433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14433
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_166_length_14433_cov_2.677285"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..711)
                     /locus_tag="PK077_02120"
     CDS             complement(<1..711)
                     /locus_tag="PK077_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23126.1"
                     /translation="MKHLYFSIMLGLAPAFCLHSARGEQPAPAEHKHEDGSACTADHG
                     HDDHQHKAGETCTQGHAHEEHRHADGAVCTGDHELEGHQHAEGEACTGNHEREESAEK
                     NSGQPGDMIPVHVDEKARHSLDMRFEKVEETSHGAAKTLHGQMVIPPHAVTTYALPAA
                     GRVTFNVKSAQQVRKGELLYTLASPDIVEMEGNASQARAALDRAAAELGTLRERRAQL
                     EKIGTRNSELNTSIQFKEA"
     gene            complement(744..2024)
                     /locus_tag="PK077_02125"
     CDS             complement(744..2024)
                     /locus_tag="PK077_02125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HRN23127.1"
                     /translation="MKSTPFFAGIGLAALLSSCSVYHSAPIDLNAEEASWKEASSVDS
                     PTSITQNQARQIGLVMNPDLNKARLKLASSNEVARQSGWWNDPSFSWDIEQVLQENTV
                     NMSGSLGFTIPVTGLPALEKKVAEQYKEADFWTLRQAELDFLSSLDQAWSKLAVTQRR
                     KTVIRERLNAIRRENGMIEQLIGAGEVDFSSRQVASQRMNDAIRELQTVTETELEQKM
                     ELVKLMGLHPSAVRKLGFLTKKNFQLPAAVPAPGPSALTASPKIKAQLATYATTETLF
                     KTEIRRQYPELELGPSFTRDEGEKEVGGEIGFNLPLWNRNRKAIAEARGTRDLSRQET
                     IQLWKTELFNARQLDASQQMALRHCRTELQRMESFAASVRTMEKLHGIGEASLLELAE
                     NRHQLYESRLAFLDNLDKLLAVQAQLRYLTHANL"
     gene            complement(2171..2599)
                     /locus_tag="PK077_02130"
     CDS             complement(2171..2599)
                     /locus_tag="PK077_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23128.1"
                     /translation="MVRALRTGIMLLTLLLVVVGGTGMVSVCPCHEEIFIFSCSCHQD
                     SAPCECHGDDVPRTGSSSPLAGHLCEHRQLEINDLTVPVPHAPLPQPYVIWQTLPDFH
                     HLARPLLLTSQTSDSATPVPPDPFRNAGPTHEGFQLPLLI"
     gene            complement(2682..3707)
                     /locus_tag="PK077_02135"
     CDS             complement(2682..3707)
                     /locus_tag="PK077_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HRN23129.1"
                     /translation="MNQHTRNIIVDSLILVAFSSLLGGLSLLVSKSFSGDPNPALDLI
                     TVMKKAEAKEVNASSEQEEKEARVKGMKEFEQTLKEGEELAAAEGKAFVNMTDPHGRT
                     PVMWVCYANYNNIETTLKLEATRAPYLDRLLEDPRVEIDRKDKDGWTALHWASWSGLD
                     RLSDMLIERKADINNREGNGFTPLMLAAMRGNFQVAALLLEKGADMTAVNKFGKNALQ
                     LAEEGALAYQSSFDLTKTEVSKAPVDSFIRAYEKAVAGLPPEHAPEFMEAMKKAVGRQ
                     SFVETGYVLQLITRSLSSKNLLTEEQSGKFREEITGAIAENSRIIDVRGVAFEHTVDL
                     LRKAAEK"
     gene            complement(3720..5201)
                     /locus_tag="PK077_02140"
     CDS             complement(3720..5201)
                     /locus_tag="PK077_02140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN23130.1"
                     /translation="MNESANPILKYFSEFKILKTVSKDFWLTNVVQFFDGMAYFSMIT
                     VFVLYLTDYCSFNDADAALWVGLYTLFISAFVFAVGSICDIIGIRRTYLIGFIILIAG
                     RLIMGFGPDLSPTVDSGRLAVMAGILIMSFGTAFMSPVIQTSIRRFTPLKARSTGFNI
                     YYLLMNISAVIANVFLIEFFRKHFGAVDGGYWIINFGTLMVLLGCITTRFINEDNYAE
                     PSEREANINAPLRRPLQLFMEVWKESAFRKLILFLVLTMGVRIVFTLQFLVMPKYYVR
                     TLYDDFAIGSINAVNPAIIVSGLILLIPVLGRFSTVGLMIAGMSISAFSLVFMAIPIE
                     WYYLVPGIETRSQAYLVAIVAQILVFAFGELLFSPRFSEYVARVAPKDKVASYMSLAA
                     LPMFIAKPINGIIGGLLVAYLCYDGICAKMDTGHIGFWDSPEFMWTIYLAMAVISPIA
                     IIMTRRTITSDHPEEDAASPPISAIEAETDPALTAEELTEANS"
     gene            complement(5332..5976)
                     /locus_tag="PK077_02145"
     CDS             complement(5332..5976)
                     /locus_tag="PK077_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23131.1"
                     /translation="MLFAVVIFLSVVVLGVIAALRFTKGIIPLRTYESRELPGFPGPA
                     GLIHRLDGAEKRQRKLEKARFPGVDENGFISLPPSWEGKVTAGRLACVAHTWAMSSLV
                     REDPEAAKARKTASARATMIPFFTALILLGMLTAGLLKFQIALAIGLACWAFFTFSAL
                     PSQFREWKAAEIAKSGLKEAGLWPVLPSDAMAVEQCLKALSWCHVAGFRRILPR"
     gene            6002..6139
                     /locus_tag="PK077_02150"
     CDS             6002..6139
                     /locus_tag="PK077_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23132.1"
                     /translation="MSVNLSGWHEAVLPGQQPGADADIVFSFFSRPERLENGGMELMS
                     E"
     gene            6278..6889
                     /locus_tag="PK077_02155"
     CDS             6278..6889
                     /locus_tag="PK077_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23133.1"
                     /translation="MRRWLPFVIMAASLGVSPAQDVASPDAADLDLDLVKLKSGPKGR
                     QREAMRKVLLLVHLKQKETWLAESTGDEAVHLAAEISFRRYLASVDMDGCPEEFRRAF
                     LRYAKLLNSFEEKDLNSVADFPAVPLQAAGRELFRVLLTYRLEPEHLLEEMKAMVDEE
                     IEGKEELSAGQMMDIVRGMREALQSGSLPFPAERPPEDRVSGM"
     gene            complement(6996..7913)
                     /locus_tag="PK077_02160"
     CDS             complement(6996..7913)
                     /locus_tag="PK077_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRN23134.1"
                     /translation="MKSTLKTLKLLADPTRLRIINVLNEESLSVAELQEILGMGQSRI
                     STQLAQLRQEGVVEDARSGKNVFYTLSLAGDLHNVALKACEELPEAETDQKALQVILD
                     KRKNRTQAYFDEVVCRLGKNYAPGRSWKALAGALLRILNYDVVADLGAGEGFVSQLIS
                     PSAKQVIAVDNSPSMVELGQELARKHGLDNLEYRLGDIEAPPIKPGTVDLALLSQALH
                     HAQKPSRALEAAWDILKSGGCLVVLDLLQHGQEEARELYADRWLGFTPAALESMLKEA
                     GFRNIHTDIVDREPDPPHFQTLMAAAWKP"
     gene            7951..9135
                     /gene="metK"
                     /locus_tag="PK077_02165"
     CDS             7951..9135
                     /gene="metK"
                     /locus_tag="PK077_02165"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRN23135.1"
                     /translation="MSRNTPHIFTSESVGEGHPDKVADYISDSILDACLAQDKTSRVA
                     CETLVKSNMVIIAGELTTKAVIDPEKIARQAIREIGYCNRQDDDVFHADTVFFTNLLT
                     EQSPDIAQGVDAREAEGKGHAEQGAGDQGIMFGFATNETPELLPAPIVFAHKLLIELA
                     RRRKRGHVDWLRPDCKSQVAVAYDEDGRPAHIANVVISTQHTEDVDHDTIYSYCVKLI
                     KNVLPAELLDERTEYFINPTGKFVVGGPHGDSGLTGRKIIVDTYGGMGRHGGGAFSGK
                     DPSKVDRSAAYMCRWVAKHIVAAGLADKCELQVAYAIGYPAPVSIRVDTFGTGKVEEI
                     SIENALENIFSFKPADMVEQLNLLRPIYRKTTHYGHFTNPELPWEQLDETRLASLKQL
                     LH"
     gene            9158..10573
                     /gene="ahcY"
                     /locus_tag="PK077_02170"
     CDS             9158..10573
                     /gene="ahcY"
                     /locus_tag="PK077_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRN23136.1"
                     /translation="MFSETEQYKVRDIGLADFGRRELDIAEHEMPGLMAVREKYAAAR
                     PLEGVRIMGSLHMTVQTAVLIETLTALGASVRWASCNIFSTQDHAAAAIAKSGTPVFA
                     WKGETLREYWDCTWKVMNFPDGLGPQLIVDDGGDATLLIHRGYEMEEGSDWVDTPSES
                     EEEEVVKALLKKIAAATPGIFHRWLPELKGVSEETTTGVHRLYQMQAAGRLLIPAINV
                     NDSVTKSKFDNLYGCRESLVDGIKRATDVMVAGKVAVVCGYGDVGKGCAQSLRGMGAQ
                     VVVTEIDPICALQAAMEGYRVLTVEDTLGWADIYVTTTGNCNIIRIEHMEKMKDQAIV
                     CNIGHFDNEIQVHKLQTYPGIRHLNIKPQVDRYTFPSGNSLYLLAEGRLVNLGCATGH
                     PSFVMSNSFANQVLAQLELWNTRKDRSVGVEVLPKVLDEEVARLHLAKIGCKLTVLRP
                     EQADYIGVPVEGPYKPDFYRY"
     gene            10576..10878
                     /locus_tag="PK077_02175"
     CDS             10576..10878
                     /locus_tag="PK077_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23137.1"
                     /translation="MSRFSFPGRSAQACPAERLFCFPDDGGKQDNGAFIFFPVFYAGS
                     LLLVKKRKTFYPVFNVQIPLQQAKREQSWNADQPEPRPALQNVPKPFEFGKKAVIE"
     gene            complement(11591..>11874)
                     /locus_tag="PK077_02180"
     CDS             complement(11591..>11874)
                     /locus_tag="PK077_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23138.1"
                     /translation="LSTKRLSCHAETTARNLFLITADLEFPNRIWNSLFLDSLKKLKL
                     FIRFHDFSHSSLLQSETFYQGTVPGYFKTGLSRRMGNNYFGRFFSGRFF"
     assembly_gap    11875..11917
                     /estimated_length=43
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12291..13157
                     /locus_tag="PK077_02185"
     CDS             12291..13157
                     /locus_tag="PK077_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23139.1"
                     /translation="MPYYKGDAPDVCSVFGNSFQFYNYILQSRERIRQLDGDYLLIIG
                     DDLLLNPRFDEFSTPSLLGIHGEDMCYLDGFVDVSLPVCYRGTAEAHHFSITPPGIDA
                     ESVNRNVPSYEEARRILKSRNLMRHDELSRVRMFLPKWSPGGIHANWKVLKGRVWHLL
                     NYWKHRIKKYQYSYPVVFGYSDIVCIPKGKLDDFCRILEVFSAWNMFVELAIPTALQL
                     LPGTKLSTLEDTQYKSGNVWFPQDPEHFEAMNAVIDGLVSSSGGDLSELLASFPEEYL
                     YLHPVKLSRFAR"
     gene            13185..14414
                     /locus_tag="PK077_02190"
     CDS             13185..14414
                     /locus_tag="PK077_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HRN23140.1"
                     /translation="MMKYAVIGAGVSGLSMAGMLLKKGHEVVVYERDSRPGGLIKCTE
                     VQGNLYHRVGGHVFNSRRQEVLDWFWSRFDKERDFVSARSRAVISLEGGAVVDYPIEN
                     HLDQFPEAVRSSIVHELLELYRNPPAEPRSLGEFFLNRFGKTLNSLYFTPYNNKVWRQ
                     DISQIAMDWLEDKLPMPSVAEILLNNIGHINESAMVHSSFFYAKNGGSQFLADTLARG
                     LKVRYRQEAVNILPKDGKWLVQGELFDRVVFTGNVRQLGDCFPCMDELRPFFPRISEL
                     RFHGTTSVLCRISPNDYSWIYMPSPSHRSHRIICTGNFSRNNNNGDITTATIEFSEQM
                     TEGEIRRQLELIPFSPVYLAHHWEEYTYPVQDVSSRTLIRELKECLEPKGIYLLGRFA
                     EWEYYNMDAAMGAASIL"
BASE COUNT         3126 a         3671 c         4224 g         3369 t
ORIGIN      
        1 cgcctctttg aattggatgg aagtattcag ttcgctgttg cgggtaccga ttttttccaa
       61 ctgggcccgg cgttccctga gggttccgag ttctgctgcg gcgcggtcca gcgcggccct
      121 ggcctgggag gcgtttcctt ccatttccac aatatccggg gatgccagcg tgtacagcag
      181 ttcccctttg cgcacttgtt gtgcggattt gacgttgaat gtcacgcgcc ctgcggcggg
      241 aagggcgtat gtggttaccg catggggagg aatgaccatc tgcccgtgaa gggttttcgc
      301 cgctccgtgg gaggtttcct ccactttctc aaagcgcatg tccagagaat ggcgcgcttt
      361 ttcatccaca tgcacgggaa tcatgtctcc cggttggccg gaattttttt ccgcagactc
      421 ctcgcgttca tggttgcccg tgcaggcttc cccttccgcg tgctggtgac cttccagttc
      481 atggtcgcct gtgcagacgg ccccgtccgc atgcctgtgt tcttcatggg cgtggccctg
      541 agtacaggtt tccccagcct tgtgctgatg gtcgtcatgg ccgtggtccg ccgtgcaggc
      601 ggaaccgtcc tcatgtttgt gttccgcagg ggcaggctgt tctccgcgtg cggaatggag
      661 acagaaagca ggagccagtc ccagcatgat ggaaaaataa agatgtttca tattcattaa
      721 agatggtgga agattgatgt ggattaaagg ttggcgtggg tgaggtagcg gagctgggcc
      781 tgaacggcca gaagcttgtc cagattgtcc aggaaagcca ggcggctttc gtaaagctgg
      841 tggcggtttt ccgccagttc cagcagggat gcttccccaa tgccgtgcag cttttccatg
      901 gtccggacgg aggcggcgaa ggattccatg cgctggagtt ccgtgcggca gtggcggagt
      961 gccatctgct ggctggcgtc aagctggcgg gcgttgaata attccgtttt ccaaagctgg
     1021 atggtttcct gtctggacaa gtcccgtgtg cctcgggctt ccgcaatggc cttgcgattg
     1081 cggttccaaa ggggaagatt gaaccctatt tctccgccta cttccttttc tccttcgtcg
     1141 cgggtgaagg aggggccgag ctccagttcc gggtactgcc ggcggatttc cgttttgaat
     1201 aatgtctccg tggtggcgta ggtcgccagt tgtgccttga tttttggaga ggcggtcaag
     1261 gctgaagggc cgggagccgg tacggcggcg ggaagctgga aattcttttt cgtcaggaaa
     1321 ccgagtttcc gtaccgcaga aggatgcagg cccatcagct tgacgagttc cattttttgc
     1381 tccaattccg tttccgtcac ggtttggagt tcccggatag cgtcattcat ccgctgggag
     1441 gctacctgcc gagaggaaaa atccacttcc cccgcgccga taagctgttc aatcatgccg
     1501 ttttcccgcc ggatggcatt cagccgttcc cggatgaccg tcttgcggcg ctgggtaacg
     1561 gccagtttgc tccatgcctg gtccagggaa ctgagaaaat ccagttcagc ctggcggagg
     1621 gtccagaagt cggcctcctt gtattgttcc gccacctttt tttccagggc ggggaggccg
     1681 gttacgggaa tggtgaagcc caggctgccg gacatgttga ccgtattttc ctggaggact
     1741 tgttcaatat cccaggaaaa ggagggatcg ttccaccagc ccgattgcct ggctacttca
     1801 ttgctggaag ccagtttcag ccgggctttg ttcagatccg gattcatgac caggccgatc
     1861 tggcgagcct ggttctgagt gattgaggta ggggaatcta cgctggacgc ctctttccag
     1921 gaggcttcct ccgcgttcag gtcgataggc gcgctgtggt agacgctgca tgagctgagc
     1981 agggctgcca ggcctattcc ggcgaagaat ggtgtggatt tcatgatgga aaagataata
     2041 aaaacagttc ggtccaatca ggtttggaca atgcggcgaa taattccgcg gaaacaaata
     2101 aaagcggccg ccgtattaaa ggcagcgtac cgcaggaaac gcccttaagg acgtttctga
     2161 aaaagagccg ttaaatcagc aggggaagtt gaaagccttc atgcgtcgga ccggcgttcc
     2221 tgaaggggtc agggggaacc ggtgttgcgg aatccgatgt ttggcttgtt aaaagaagag
     2281 gccgggccag atggtggaaa tccggcaatg tctgccagat gacataaggc tggggcagag
     2341 gtgcatgagg aacgggaacc gtcagatcat taatctccag ctgccggtgt tcgcagaggt
     2401 gcccggccaa agggctggaa gagccggtgc ggggcacatc gtccccgtgg cattcgcagg
     2461 gggcggaatc ctgatggcag gaacaggaaa aaatgaaaat ttcttcatgg cacgggcaca
     2521 cggaaaccat acccgttccg cctacgacga ccagcagaag ggtgagcagc atgatgccgg
     2581 tgcgcaatgc tctaaccatg gtatgccgcg aatcctagaa aggagccgtg aaagcgtcaa
     2641 gagcaaaagt ggcggatgtc tgaaccctga aggaaaggcc gttatttttc cgccgctttg
     2701 cggagcaggt ccacagtgtg ttcaaaagct acgccgcgga catcaatgat ccgggagttt
     2761 tccgcgattg ctccggtgat ttcctcccgg aatttaccgg actgttcttc cgtgagcagg
     2821 tttttggagc tcagggagcg cgtaatgagc tggaggacgt accctgtttc cacgaaagac
     2881 tggcgtccca cggctttctt catggcttcc atgaattccg gggcatgttc gggcggcagg
     2941 ccggccacgg ccttttcata agcacggatg aaggaatcca ccggggcttt ggaaacttcc
     3001 gtcttggtga ggtcaaagct ggattgataa gcgagagctc cttcttccgc cagttgaagg
     3061 gcgtttttcc cgaatttgtt gacggcagtc atatccgctc ctttttccag cagcagggcc
     3121 gccacctgga aattgccgcg catggctgcc agcatgaggg gcgtaaagcc gttgccctcc
     3181 cggttgttga tgtctgcttt cctttcaatc agcatgtcgg acaggcggtc cagcccgctc
     3241 caggaagccc agtgcagcgc ggtccacccg tccttgtcct tccggtcgat ttccacgcgg
     3301 gggtcttcca gaagccggtc cagatagggg gcccgagtgg cttccagctt cagcgtggtc
     3361 tcaatgttgt tgtagttggc gtagcatacc cacatgacag gagttctgcc atggggatcc
     3421 gtcatgttga caaaagcctt tccttctgcc gcagccagtt cttccccttc cttcagggtt
     3481 tgttcgaatt ccttcattcc cttgactcgg gcttccttct cctcttgttc ggaggaagcg
     3541 ttgacttctt tggcctccgc tttcttcatt acggtgataa ggtccagggc cgggttggga
     3601 tctccggaga aagatttgga gaccaacagg ctgagaccgc cgaggaggga gctgaaagct
     3661 accaggatga gggaatccac aatgatgttg cgcgtgtgct ggttcatggt ctcgtaaagt
     3721 caggagttgg cttccgtaag ttcttccgcc gtcagggcgg gatccgtttc cgcttcaatg
     3781 gcgctgatgg gtggcgatgc cgcgtcttcc tcgggatggt ccgaggtgat ggtcctgcgg
     3841 gtcatgataa tggcgatggg gctgattacg gccatggcca ggtaaatggt ccacatgaat
     3901 tccggggagt cccagaagcc gatgtgcccg gtatccatct tggcgcagat gccgtcatag
     3961 cagaggtagg cgacgagcag gccgccgatg atgccattga tgggtttggc gatgaacatg
     4021 ggaagcgccg ccagagacat gtaggaggcc actttgtctt tcggagctac ccgcgccaca
     4081 tattcggaga agcgggggga gaagagcagt tccccgaatg cgaataccag gatctgcgcc
     4141 acaatggcta ccagatacgc ctgggagcgc gtttcgatgc cgggcaccag gtagtaccat
     4201 tcaatgggaa tggccatgaa aacgagagag aaagcggaga tggacatgcc cgcaatcatg
     4261 aggccaacgg tagagaagcg gcccagcacc ggaatgagca ggatgaggcc ggagacgatg
     4321 atggccgggt tgacggcatt gatggagcct atggcaaaat cgtcatatag cgtccgcacg
     4381 tagtatttgg gcatcaccag gaattgtagc gtgaaaacga tgcgcacgcc catggtcagc
     4441 accaggaaga ggataagttt gcggaaggcg gattccttcc atacctccat gaagagctgg
     4501 agaggtctgc ggaggggggc attgatgttc gcctcccgtt cgctgggttc cgcgtagttg
     4561 tcttcattaa taaaacgggt ggtgatgcag cccagcagca ccatcagcgt tccgaagttg
     4621 ataatccagt atccgccgtc cacggcgccg aagtgcttgc ggaaaaattc aatcaggaac
     4681 acgttcgcaa tgacggcgga aatgttcatc agcaggtagt agatattgaa gcccgtggaa
     4741 cgcgctttca gcggggtgaa gcgccggatg gaagtctgga tgacggggga catgaacgcc
     4801 gtgccgaagg acataatcag aatgcccgcc atgacggcca gccttccgga gtccacggtt
     4861 gggctgaggt ccggaccgaa gcccatgata aggcgcccgg caatgaggat gatgaagccg
     4921 attaggtaag tgcgccgtat gccgatgatg tcgcagatgg aacccaccgc gaagacgaag
     4981 gcggagatga aaagggtgta caggcctacc cagagggcgg cgtccgcatc gttgaagctg
     5041 caatagtccg tcaggtagag gacgaatacc gtaatcatgg aaaaataggc cattccgtcg
     5101 aagaactgga cgacgttggt aagccagaaa tccttggata cggttttcag gatcttgaat
     5161 tccgaaaaat atttgaggat ggggtttgcg gactcattca tggagagatg gaaacggtaa
     5221 ggaaaagttg acgacgatga aagagtgggt tacacgcatc cccgtttcaa gatttttttc
     5281 tcccaaaggc cggtttgacc ggaaaaatgc gcttcatcgc cggggaaacc gctatctggg
     5341 cagaatgcgt cggaagcctg ccacgtggca ccatgacaac gctttcaggc attgttccac
     5401 agccatggcg tccgaaggca gcacgggcca cagcccggct tcctttagcc ctgatttggc
     5461 tatttccgcc gctttccatt cgcggaactg ggagggaagg gcggagaaag tgaaaaaggc
     5521 ccagcaggcc agcccgatgg ccagcgcgat ctggaatttc aacagtccgg ccgtcagcat
     5581 tcccagcagg ataagagctg tgaaaaaagg aatcatcgtg gcgcgcgcag aggcggtttt
     5641 gcgggctttt gccgcctccg ggtcttcccg gaccaggctg ctcatggccc aggtatgggc
     5701 tacgcaggcc agccggcctg cggtcacctt gccttcccat gacggaggaa ggctgatgaa
     5761 tccgttttca tccactccgg gaaaccgggc tttctccagt ttccgctggc gtttttccgc
     5821 accgtccagg cggtggatta atcccgccgg gccggggaaa ccgggcagtt cccgggattc
     5881 gtaggtgcgc aggggaatga tgcctttggt gaaacgcagc gcggcaatga caccgagcac
     5941 gaccacggaa aggaaaatca ctactgcgaa tagcatagac ggctttttct catatgcccg
     6001 tgtgtccgtc aatctctccg gctggcatga agccgtcctt cccggccaac agccgggtgc
     6061 ggatgcggac atcgtttttt ccttcttttc ccgcccggaa cgcttggaaa acggagggat
     6121 ggagcttatg tcggaatgat ctcggttccg ggtgttcctc tgggcgggca caacattccg
     6181 gcatggattt tcttcatgtc ggaggcgctg gatttgggtt gattttccgg cgtttttcat
     6241 gtaatggctg accgcaatgg tccatattcg ggaatgcatg aggaggtggc tgccgtttgt
     6301 cataatggcg gcttccctgg gtgtgtctcc cgcccaggat gtggcgagcc ccgatgccgc
     6361 ggatctggat ttggacctgg tcaaactgaa aagtggcccc aagggcagac agagggaggc
     6421 catgaggaag gtcctcctgc ttgtgcatct gaagcaaaag gaaacgtggc tggcggaatc
     6481 cacgggggat gaggccgtgc atctggcggc ggagatttcc ttccgccggt atttggcttc
     6541 agtggatatg gacggatgcc cggaggaatt caggcgcgct ttcctgcggt atgcaaagct
     6601 cctgaattcc tttgaagaaa aagatttgaa cagtgtcgcg gattttcctg cggtcccttt
     6661 gcaggctgcc ggaagggaac tgttccgggt gttgcttact taccgtctgg aaccggagca
     6721 tctgctggag gaaatgaaag cgatggtgga tgaggaaatt gaggggaagg aggagctgtc
     6781 tgcaggacag atgatggata ttgtccgggg aatgcgggaa gccctgcaat ccggaagcct
     6841 cccctttcca gcggaaaggc ctccggaaga cagggtttcc ggaatgtagc ggcttttcat
     6901 ggatggcatg ccggtaccgt gaggaatctt gtggaggcat gccggaagtc cgctccggct
     6961 attccggaag gaaaatggat taagacggct gtacgtcagg gtttccaggc ggcggccatc
     7021 agagtctgga aatggggcgg gtccggctcc ctgtccacaa tatccgtgtg gatgttccgg
     7081 aatcccgctt ccttcagcat gctttccagg gcggcgggcg tgaagcccag ccagcggtcc
     7141 gcgtaaagtt cacgggcttc ctcctgtcca tgctggagca gatccagcac caccaggcaa
     7201 ccgccggatt tcaggatatc ccacgcagct tccagcgctc tggacggttt ttgcgcatgg
     7261 tgcagggcct ggctgagcag ggccagatcc accgtgccgg gtttaatggg cggcgcttcg
     7321 atgtcgccca ggcggtattc cagattgtcc agcccgtgtt tgcgcgccag ctcctgcccc
     7381 agctccacca tgctggggga gttatccacg gcgatcacct gtttggcgct gggggagatg
     7441 agctgggaaa cgaaaccttc cccggctccc aggtccgcca ctacgtcata gttcagaatg
     7501 cggagcagtg ctccggccag cgctttccag gaacggcctg gcgcgtagtt cttgcccaga
     7561 cggcacacta cttcgtcaaa ataggcctgg gtgcggtttt tgcgcttgtc caggataacc
     7621 tgaagggctt tttggtccgt ttccgcctcc ggcaattcct cgcaggcttt cagggctacg
     7681 ttgtgcagat cgcctgctag ggaaagagtg taaaagacat ttttgccgga acgggcgtcc
     7741 tccaccaccc cttcctgccg gagctgggcc agctgggtgg aaatgcggct ttgccccatg
     7801 ccgagaattt cctgaagttc cgcgacgctc agggattctt cattcagaac gttgatgatg
     7861 cgcaggcggg tgggatctgc caggagtttg agcgttttta gggttgactt catagcggaa
     7921 taatatatca acacatcgag atttatcgat atgagtagaa acacccctca catctttacg
     7981 tcggaatccg ttggagaagg ccatccggac aaggtggccg actacatttc cgactctatt
     8041 ttagacgcct gcctggctca agacaaaact agccgcgttg catgtgaaac gttggtgaag
     8101 agcaatatgg tcatcattgc cggagagctg acgaccaaag cggtgatcga tccggaaaaa
     8161 atcgcccgcc aggccattcg ggagatcggg tactgcaaca ggcaggacga cgacgttttc
     8221 catgcggata ccgtcttttt cacgaatctg ctcacggaac agtccccgga tattgcccag
     8281 ggggtggacg cccgggaggc ggagggcaag ggccatgcgg agcagggcgc cggagaccag
     8341 ggcattatgt tcggcttcgc aacgaatgag acgccggagc tgcttccggc ccccatcgtg
     8401 tttgcgcaca aactgctcat tgagctggcc aggaggcgca aacgcgggca tgtggactgg
     8461 ctgcgtccgg actgcaagtc ccaggtggct gtggcctatg acgaagacgg gcgtcccgcg
     8521 catattgcaa acgtagttat ttccacccag cacacggaag acgtggacca tgacacgatt
     8581 tactcatatt gcgtcaaact cattaaaaat gtgcttcctg cggagcttct ggatgagagg
     8641 acggaatatt tcatcaaccc caccgggaaa ttcgtcgtgg gcggtcccca cggggattca
     8701 ggcctgacgg gccgcaaaat tattgtggac acgtacggcg gcatggggcg ccacggcggc
     8761 ggagcctttt ccggaaaaga cccgtccaag gtggaccgtt ccgccgcgta tatgtgccgc
     8821 tgggttgcca agcacattgt ggctgccgga ctggcggata aatgtgaatt gcaggtggct
     8881 tacgccatcg gttaccccgc tccggtttcc atccgggtgg atacgttcgg taccgggaag
     8941 gtggaggaaa tatccattga aaatgcgctg gaaaacatct tttccttcaa gcctgcggat
     9001 atggtggaac agctgaacct gcttcggcct atttaccgga agacgacgca ctacgggcat
     9061 ttcaccaatc cggagctgcc ctgggaacag ctggacgaaa cgcgcctggc ctccctgaaa
     9121 caacttcttc attaatccta cgacaacgct tacagacatg ttttcagaaa cggaacaata
     9181 caaggtgcgc gatatcggcc tggcggattt cggacgcagg gaactggata ttgcggagca
     9241 tgagatgccc ggtctgatgg ccgtgcgtga aaagtacgct gcagccaggc ccctggaggg
     9301 tgtgcgcatc atgggctccc tgcacatgac ggtgcagacg gccgtgctga tagaaacgct
     9361 gacggctctg ggcgcttccg tccgctgggc gagctgcaac attttctcca cccaggacca
     9421 cgccgccgcc gccatcgcca aatccggcac ccccgttttc gcctggaagg gggaaacgct
     9481 gcgggaatac tgggactgca cgtggaaggt catgaatttt ccggacgggc tggggccgca
     9541 gctgattgtg gacgacggcg gagacgccac cctgcttatc caccgcgggt atgaaatgga
     9601 ggaaggctcc gactgggtgg ataccccgtc ggaatcggaa gaagaggaag tcgtcaaggc
     9661 cctgttgaaa aaaattgccg cggcgactcc gggcattttc catcgctggc ttcccgagct
     9721 gaagggcgtt tccgaggaaa cgaccaccgg cgtgcaccgc ctgtaccaga tgcaggccgc
     9781 cggccgtctg ctgattccgg ccatcaacgt aaacgattcc gtaaccaagt ccaagtttga
     9841 caacctgtac ggctgccgtg agtcccttgt ggacggcatc aagcgggcca cggacgtgat
     9901 ggttgccggg aaggtggccg tcgtctgcgg ctacggggac gtaggcaaag gctgcgccca
     9961 gtccctgcgc ggcatgggag cccaggtggt cgtgacggaa atagacccca tctgcgctct
    10021 tcaggcagcc atggaaggtt accgcgtact gacggtggaa gacacgctgg gctgggcgga
    10081 tatttacgtg accactacgg gaaactgcaa catcatccgc atcgaacaca tggaaaaaat
    10141 gaaggaccag gccattgtct gcaacatcgg ccacttcgac aatgaaatcc aggtacacaa
    10201 gctccagacg tatcccggca tcaggcatct taacatcaag ccgcaggtgg accgctacac
    10261 gttcccgtcc ggcaacagcc tgtatcttct ggcggaaggc cgcctggtca acctgggctg
    10321 cgctacgggt catcccagct tcgtgatgtc caacagcttc gccaaccagg tgctggccca
    10381 gctggaactg tggaataccc gtaaagaccg ttccgtgggc gtggaagtgc tgcccaaggt
    10441 gctggatgag gaagtggcgc ggctgcacct cgccaagata gggtgcaagc tgactgtcct
    10501 gcgtccggag caggcggact acatcggcgt tccggtggaa ggtccttata agccggactt
    10561 ttacaggtat taaccttgtc aaggttttct tttcctggcc gttccgcgca ggcctgtccg
    10621 gcggaacggc ttttttgttt tcccgatgat ggagggaagc aggataacgg tgcgtttatt
    10681 ttttttcctg ttttttatgc aggttccctg ctgttagtga aaaaaaggaa gactttttat
    10741 ccggtcttta atgttcaaat acctttgcag caggcaaaac gggagcaatc ttggaatgct
    10801 gaccagccag agcctcggcc tgcattgcag aatgttccaa aaccatttga gtttggtaag
    10861 aaggctgtta tcgaatgaag cataggggaa gagcaggagc cttctctgtt ccgcctgaat
    10921 cagactcatc aggcgatcgg cggcctggta tgttatttcc ccggacttat gctttccgta
    10981 tacctctttc atgatcatta tccaggttgt tatcaggaat tccgatctct tgatcaggat
    11041 tgtcctgggg ttcccgctct taaaattgca gatattactg ttgtgtctcc gatagtacga
    11101 acaccgtttg ccggatgcga ccatgcctga ttttttcaaa taggcgcgca tggacagcat
    11161 tgtatcgtcc gcaatgccgg gccctgaagg caaatattcg aagtcgctga acaaagacct
    11221 gtggtacatg gcatttccgc caaaacagct gtaaacatgg gaaatgtcaa aaatacattc
    11281 cctgtagggt ttgacgacga cggaatctga atctggcaac atgcgtccat aagcgggttg
    11341 tccatcttca tcgatttcat tataagaatg gcagtaaacc agatgaccgg gatatttttc
    11401 cgccagagat gccaacgagg agatcctgtc ttcccgtgcg atgtcatcgc cgtcaaactt
    11461 gataatccat tccccccggg acaaatgaac ggcacattgc aaattccctg caattccccg
    11521 gttgctttcg ttacggtgga gaataatacg ccgtccttct ccctcttttt ccaccatttc
    11581 ttccaggcat tcaaaagaac cgtcctgaga aaaatcgtcc gaaataatta tttcccattc
    11641 tcctggataa tcctgtttta aaatagcccg gaactgttcc ttgataaaac gtttctgatt
    11701 gtaacaaaga agaatggctg aaatcatgaa atcgaataaa cagtttaagt ttttttaaac
    11761 tgtctaaaaa taacgaattc caaattcgat tggggaattc caaatccgct gtaattaaaa
    11821 agagattgcg tgcggttgtt tctgcatgac aggagagcct cttcgtagaa aggannnnnn
    11881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngca ggtatggatg tttctgccgg
    11941 aatgggaatt gatggggtta cccggatgga atcatgaaga cggaagattc cggtcattgg
    12001 ggaagcttat tttcagaagt tcctttcttt caaaatttaa ccttgactcc ggatttggaa
    12061 tccgtgtaat atgccccgca ctctttttac tgtgcgtgca ctcagagagc aaatcccttt
    12121 ttttcaataa tttatgttga agggtacggt ttgtttctca acatcaaacg gccatggata
    12181 cttcttcccg gaaaacgtct cttgttgtat tgttcaacca tcagtatgaa cgtaacattc
    12241 cggtaatcag agagctttat tcaagacgct tttccgggtt gcttcaatta atgccctact
    12301 acaagggaga tgcccctgac gtatgttccg tcttcgggaa ttctttccag ttctataatt
    12361 atattttgca gtccagggaa agaatcaggc agttggatgg cgattatctt ctcatcattg
    12421 gagacgatct tcttttaaat cccaggtttg acgaattttc aacgccgtcc ctgctgggaa
    12481 tccatgggga ggatatgtgt taccttgacg gttttgtgga cgtttctctg ccggtgtgtt
    12541 accggggaac ggcggaagcc caccattttt ccataacccc tcccggtatt gatgcggaaa
    12601 gcgtcaacag gaacgttccg tcctatgagg aggccaggcg gattttaaaa tccaggaacc
    12661 tcatgcggca tgatgaattg tcgcgcgtac gcatgtttct gcccaaatgg agccccgggg
    12721 ggattcatgc gaattggaaa gtgttgaagg gcagggtatg gcatctgctg aattactgga
    12781 agcaccggat taaaaaatac cagtattcct atcctgtcgt tttcggatat tcggacattg
    12841 tctgcattcc gaaagggaaa ttggacgatt tctgccgtat ccttgaagtg ttctccgcat
    12901 ggaacatgtt tgtggagctg gccattccca cggcgttgca gcttcttccc ggaacaaagc
    12961 tgtctacgct ggaggacacg cagtacaagt cgggaaacgt ctggtttccc caggatccgg
    13021 aacactttga agccatgaat gccgtcattg acgggctcgt gtcctcttcc ggaggagacc
    13081 tcagcgagct gcttgcctcc tttccggagg aatacctgta tctccatccc gtgaaattgt
    13141 cccgtttcgc cagatagtgg cgttcataaa ttgtttttta tcccatgatg aaatatgctg
    13201 tgatcggagc cggggtttcc ggattgtcca tggccggaat gttgctgaag aaagggcatg
    13261 aggtagtcgt ttatgagagg gactcccggc ccggcggcct gattaaatgc acggaagtac
    13321 aggggaacct gtatcatcgt gtgggaggcc atgtgttcaa ttcccggcgg caggaagtgc
    13381 tggactggtt ttggtcccgg tttgacaagg agcgcgattt tgtttccgcc cggagccggg
    13441 ctgttatttc cctggagggc ggggctgtgg tggattatcc cattgagaac cacctcgacc
    13501 agtttcccga ggccgtccgt tcctccatcg tccatgagct gctggaactt tacaggaatc
    13561 ctcccgcgga gccccgctcc ctgggtgagt tttttctgaa ccgtttcgga aaaaccctga
    13621 acagccttta ttttacgccg tacaataaca aagtgtggag gcaggatatc agccaaattg
    13681 ccatggattg gctggaagac aagcttccca tgccgagcgt ggcggaaatc ctgttgaaca
    13741 atattgggca catcaatgaa agcgccatgg tgcacagttc tttcttttat gcgaaaaacg
    13801 gcggttccca gtttctggcg gatacgctgg ctcgcggcct caaggtcagg tatcggcagg
    13861 aagctgtaaa tatcctcccg aaggacggga aatggctcgt acagggagaa ttgtttgaca
    13921 gagttgtctt tacggggaat gtcaggcagc ttggggattg ttttccctgc atggatgaat
    13981 tgcgcccgtt tttcccccgg atttcagaat tgcgctttca cggaaccact tccgtgcttt
    14041 gccggatttc ccccaatgat tacagctgga tttacatgcc ctctccatcc caccgttctc
    14101 accggatcat ttgcacgggg aatttttcca gaaataataa taacggggac atcaccaccg
    14161 cgactattga attttccgag caaatgacgg aaggggaaat caggcgccag ctggagctta
    14221 ttcccttttc ccctgtgtat ctggcccatc actgggaaga atatacctat cccgttcagg
    14281 atgtttccag ccgaaccctc atacgggaac taaaggagtg cctggaaccg aaaggcattt
    14341 atttattagg ccgttttgcg gagtgggaat actacaatat ggatgcggcc atgggggcgg
    14401 catccatatt gtagtattcc cactccgcaa aac
//
LOCUS       DAOMSU010000026        14420 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_168_length_14420_cov_2.823738, whole genome shotgun sequence.
ACCESSION   DAOMSU010000026 DAOMSU010000000
VERSION     DAOMSU010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 14420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14420
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_168_length_14420_cov_2.823738"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK077_02195"
     CDS             <1..220
                     /locus_tag="PK077_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRN23141.1"
                     /translation="IPPKRSASILDTFSCLVQGVIPYGAQMLMAAGISQVSPLLVMKY
                     LYYPLILGICSTLAIALAPGGTERRGKR"
     gene            331..1578
                     /locus_tag="PK077_02200"
     CDS             331..1578
                     /locus_tag="PK077_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23142.1"
                     /translation="MSLHIEPTEDAVETLRKERRKNYIAALATSILSVVLAGAILYSL
                     TIIIAPPEEPKVVGYITPDDAPPSDTPPPPEVQRETSSSSHAETPVKVVVAAAAAPVS
                     LPKIDIDPPDEPVMLEEGTALGMGDGFGPDLGDSTSAFGTSQPSGSTLVGTFYDTKQT
                     PGGRPTNMNMNQYREFLSRFVNKGWNESELNRFYKAPQQLYAAQFYIPRTPAKDAPKA
                     YGCDDKVKPSQWLAIYRGKVRAPKSGTFRFVGLGDDYLVVRFNKQNVFDYGWESASLG
                     KMTANNAKWLDAMEGKPGNDDLKKELREVGINVPPVTFYKYSSSGHWNNTMRGVAAGK
                     QFTVEQGKVYPIEILVSEGPGGEFGMTLLLEEVGMAPMSKDPKTGAPILPLFRTNYGV
                     PKPDKNKEHVPFDEIGIVWESIK"
     gene            1678..2598
                     /locus_tag="PK077_02205"
     CDS             1678..2598
                     /locus_tag="PK077_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HRN23143.1"
                     /translation="MQPFRFHSIYQPRIWGGQSMRTMLGRELPDRETAYGEAWEISDR
                     PEAMSIVKDGEWEGMPLHRLWEEHRQEIFGPGYERFPRFPLLCKILDARENLSVQVHP
                     PERTAAARQGEVKNEIWYVLHASPDALIYGGMKENATLPDIRHAAETGQMEQLIRSTH
                     LEPGEHLYIPAGLVHAIGAGHLIAEIQQNSDTTYRLYDWNRTDDSGRGRELHLEQALD
                     SIGEFRKLSQRPGYLTEMPHFTTKEYRLDKGERFTQPDSSRFAVFTVLCGSVLWDGKT
                     ARKGEFILSPAGADAVTAAEADTVLLSTTV"
     gene            2746..3477
                     /locus_tag="PK077_02210"
     CDS             2746..3477
                     /locus_tag="PK077_02210"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRN23144.1"
                     /translation="MSNISTIATYLARFSGQLQPMLEKVQGMDRRTFLLETAALLALT
                     SCSEPSGKAGMAPMISFKPSGVPVVKRSPRQLLAECNVRPMFMPKSSPLRRRSSRMKP
                     RFITMHNTENPSADAMQHARALNNGALRCNWHYTVDPYVTMQHIPLNETGRHADRGGP
                     GDMYSIGIEMCEKRGQSIVKTFDRAAKLAAYNMYAHDIPLRNVVPHYYWTGKRCPHLL
                     LDNGKPGFKWSWFISRVDYYYRCIS"
     assembly_gap    3782..3791
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3792..4226)
                     /gene="ispF"
                     /locus_tag="PK077_02215"
     CDS             complement(<3792..4226)
                     /gene="ispF"
                     /locus_tag="PK077_02215"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HRN23145.1"
                     /translation="MKNMIALTGIGYDIHRFAEIPRPLMLGGVHIPSSRGLDGHSDAD
                     VLCHAIADALLGAAGLPDIGYWFPPGDPACKDIPSLDIVAKAVSLIRERGGRIVNVDS
                     SLIAESPRISPYLEQMKGALGTALGISPVRVGIKATTNEQLGA"
     gene            complement(4253..5017)
                     /locus_tag="PK077_02220"
     CDS             complement(4253..5017)
                     /locus_tag="PK077_02220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRN23146.1"
                     /translation="MRHSLEMTTAIQAAKSAGAFLKKHFYEQKKVDEASQNDIKLELD
                     KLSQKLITEEILSVFPNHAVLGEEGYTGDRNSECEWIVDPIDGTVNYFYTIPWFCVSI
                     ALRRRGEVVLGVIYDPMMDECWHVEKGGIPYMNDHPMHCSRRERMAEAVVFVGHGKTD
                     GSKEKGIERFARIAWQVRKVRNNGSAALALAYIACGRFDAYVESVISIWDVAAGVLLV
                     EAAGGKVVLEPKEDNPEQFAIAAWNGRIPIMEALGE"
     gene            complement(5046..6839)
                     /locus_tag="PK077_02225"
     CDS             complement(5046..6839)
                     /locus_tag="PK077_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRN23147.1"
                     /translation="MENRRKRWYDLFGLLPRNLGRLLLWFSLIPIAFIIVLLVYAWRA
                     DQYELDEVLAPLENCAAYDRNGQWIGTLTDHDRVYVARNELPDNLVNAFIAREDEDFF
                     EHGGIVYSSIIRSICRNLTTLSYAQGASTITMQLARNCYELGGKTLDRKVLEMAVARR
                     IEGKYSKDEILTAYLNRIYFGQQCYGIAQAADLYFGKKVADLTLAECATLAGLVRGPS
                     IYNPVYSPEAAAKVRNATLERMFECEFITQEQLWQALREPMAVAGKREARPGSYPVLV
                     VSRELGDLDCCDEQEGTSSIFVMTTLDLEFQRMVEDVSEPMLAALESSSVWAGLPKRV
                     DNQLNRCVQAAILCVDSRKGELLAVTGGRSAQDGLDRWQSRVMPGDLFTPIVNLCAVD
                     QRRTVIRSNPEVTGRGVGFNTVIETAQKAGYKGELPRSSDLYTGRFSVPLSHAVNALY
                     LIGNDGLNVSISSVRQVGTTKKNLVMVNAPSPEESRREILPRESAHLVASLPPFRYDE
                     RTRKTFVNVKLPGNTGHFSAVMGRYFTVFAWVGFDSPEAAVYEKKGVSAALAKTCSSL
                     AGEVYDRAEEGRRKAVRERRERENAAEQGPQ"
     gene            complement(6863..9295)
                     /locus_tag="PK077_02230"
     CDS             complement(6863..9295)
                     /locus_tag="PK077_02230"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN23148.1"
                     /translation="MKAFFSINAGLITLAMFSAAQENIPDAAPIADGPLVANPEQDTL
                     DMADMLYKQAQAPATKENRQEYGRLLDLSLRKYLEFTQRFPQSAQAPLAEYRAAMCLE
                     ELGRKDEAHGLFLRLIQTGSPALVAASAYRLATDASSAGEIDKAIQYYQLVIRNAEQN
                     DLKVDAQYRLGRLFLSSGNPEAAATMFCAVMGNPEADAKFVLVSRMGYAALCADTGRL
                     GEAYSEYRKVLETPGVDNRNRGIATLQAAMLATKLKKTAEAQGLYERLLKDESLKEMA
                     PEARMGLLLGLYNMGKYREILSQYEQQKGIKMPTKDGQVRLLMLLGQSAYKLKEYRKA
                     ADFFLEAEKSVPYTQEAMQASFYRLLCYNELKQKDLPQRAQSFLNHYAKAFPTSELHD
                     MVRLMAAENLFSSNPADAARFYASIDFDKVPPKMRADILYKSAWAIAQAGNRGVAAKL
                     LTDFINDFPKDPRICEALTLRGDMYAKTKKEAEALMDFDRVIARWPKAESAAAAWQRA
                     AQIYAGRQDMANMAKYYEGLIQNFPKASPAALAEAHFLLGRAAFDQGDFKSSISHMAE
                     AKTLDPQKYGEQVNVLSVLSYHKLQDVNKLKEALETLQKENPSAVARVPDVIPAWLGL
                     QAYGMKDLETADKYMTWATQNDQLQNVKKVIWRNLAKVRLALRKYDRALVASNNFLKD
                     EDQPYRRADGMLDKASILLGLGKYADARKTAEDALALGVEGPLMASLKIVLGDISYAE
                     KKFDEAAKHYGVTAELFVNDAELKPKALFKAAEALDKAGRKSEASQYRGRLQKEFPDW
                     KQDGESLPPDAR"
     gene            complement(9292..9738)
                     /locus_tag="PK077_02235"
     CDS             complement(9292..9738)
                     /locus_tag="PK077_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRN23149.1"
                     /translation="MKFHYKMPSPIGFQLAPMLDIVFLLLVFFIVTQTFEDDEPDLSI
                     NLPSAETPKPGENVSNEIIVNIRKDGTVVINRQQYTMPQLEDKLFSVARLDKTMLVRI
                     RVDEKAESGVVVHVMDACLKAGLNNVSFSTRPPAPSSINVSNSQAS"
     gene            complement(9778..10569)
                     /locus_tag="PK077_02240"
     CDS             complement(9778..10569)
                     /locus_tag="PK077_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRN23150.1"
                     /translation="MNPVPFTTLAAAMDFVVGDRNYPLSELFMKGGFIMWPLLLLSIA
                     GVVVLVMCCFSTRASAVLPTKLVEQAESFIRKRDYTGLSILCKNGDSCYARVMLTVAN
                     FMLRNPSAQFEEVREIASAEGGRQAGFLSRQISWLSDIGAVAPMLGLLGTVVGMMKTF
                     FEIANGDFSGGKQRIDMAGGVAEALITTAGGLMLGIPAILAYVYFRSRVHKRVGDLEA
                     AVTHSVSVVATQLQKPRVGAAGAFHEEELPKVPVDPTSLRDVRGL"
     gene            10681..12249
                     /locus_tag="PK077_02245"
     CDS             10681..12249
                     /locus_tag="PK077_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRN23151.1"
                     /translation="MADREQPTLKELWRETPHKPGVYIMKDARGNTIYVGKAKDLHRR
                     LGNYFSPTGATLSNHKTRALINAIASFDYFETRNDQEAFLLESKLIKQYRPHYNIQMK
                     DDKRYPLLKIPKGEKLPRFQLARVRKDDGARYFGPFVHSQALYATQEWLNRHFRLRTC
                     KTKNPGIHDFRHCHADVIRNCSAPCVGRISINDYNRNFDQAVRLLEGTGKKSALDELT
                     REMMEAADELDFERAAYLRDIRDNLVKVLEPARRFRKGTPDLPGTVHPEEDMKELGVA
                     LGLESPPLIMECFDISNVSSNHIVASMVRFTNGRPDNKAYRRYRIRTVDGQNDFASMS
                     EVIRRRYSRILAESDAVASRQADMTLYQWLKKLSAEGKAPIKVPDLVVVDGGKGQLSS
                     ALADLEAIGLGDMPIVGLAKQREEIFFPHQSQPLCLPHSTGALKLMQRIRDEAHRFAN
                     GYNELLYRKRMRESALDDAPGMSASKKRLLLEKFKSVTAIKKADPASIAAIRGISETW
                     ARTLLNYLNSSSNS"
     gene            12256..12693
                     /locus_tag="PK077_02250"
     CDS             12256..12693
                     /locus_tag="PK077_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HRN23152.1"
                     /translation="MQFLILAAGKPSLGYAKEGVELYLNRLRPFGKTELKLVRDGNSQ
                     DVSKRLLAASEGCLRIAMDERGELWTTKKLVDLAKDWQMHSVRRIAFLIGASDGHTEE
                     LRSRCNHILSLSKFTLQHELALVVLLEQLYRCHTILAGTPYHR"
     gene            complement(12922..13317)
                     /gene="rpsI"
                     /locus_tag="PK077_02255"
     CDS             complement(12922..13317)
                     /gene="rpsI"
                     /locus_tag="PK077_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRN23153.1"
                     /translation="MSQTTPYNATGRRKTAIAHAWLTEGSGRITINRRGFEEYLPTVQ
                     LQNAVLQPFQVTNTMNKFDVNVVTKGGGIHGQVGAIRMAIARALVQVDEASRSQLREF
                     GLLTRDSRMKERKKPGRPGARKRYQFSKR"
     gene            complement(13332..13760)
                     /gene="rplM"
                     /locus_tag="PK077_02260"
     CDS             complement(13332..13760)
                     /gene="rplM"
                     /locus_tag="PK077_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRN23154.1"
                     /translation="MKTFSAKPQEVERKWYVIDAADKVLGRVAVEAANILRGKNKTIF
                     TPHVDCGDFVIIVNADKVVLTGNKENAKIYTRFSGYVGGKQVDTPRKIRARRPELLLE
                     LAVKGMVPHTRLGRQQMTKLKVYAGACHPHEAQQPTAITL"
     gene            14313..>14420
                     /locus_tag="PK077_02265"
     CDS             14313..>14420
                     /locus_tag="PK077_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRN23155.1"
                     /translation="MEVEGTICAVLAGTMFKVRLPNGHEVLAHISGKMRK"
BASE COUNT         3527 a         4197 c         3605 g         3081 t
ORIGIN      
        1 tatcccaccc aaacgcagcg ccagcattct ggacaccttt tcctgcctgg ttcagggagt
       61 catcccttac ggggctcaaa tgctcatggc cgcaggaatc tcccaggttt ctcccctgct
      121 ggtcatgaaa tacctgtatt atcccctgat tctgggcatc tgctccaccc ttgccattgc
      181 gctggcaccg ggagggacgg aaagacgggg gaaacgctga aaagcccata cggagaaagg
      241 acggcaaggg cgcctttttt gttaccgaca tgttatccct gttatcttca tgttactcaa
      301 catatgggat tcttgtgcta tcatacatgc atgagccttc atatagaacc cacggaagac
      361 gcagtggaaa ccctgcgcaa ggaaaggcgg aaaaattata ttgccgcatt agcgacatcc
      421 attctctccg tcgttttggc aggagccatt ctctactccc tgaccatcat catcgcccct
      481 ccggaagaac ccaaagtcgt cggctacatc acgccggacg atgcgccgcc gtcggatacg
      541 cctccgccgc cggaagtaca gagggaaact tcctcttcct cccatgcgga gacgcccgtc
      601 aaggtcgtgg tggcagcggc cgccgctccg gtgagcctcc ccaagatcga cattgacccg
      661 cccgatgaac ctgtcatgct ggaggaggga accgccctcg gcatggggga cggcttcggc
      721 cccgacctgg gagactccac ctctgctttc ggtacgtccc aaccctccgg aagcacgctg
      781 gtagggacct tttacgacac caagcagact cctggcggac gtcctaccaa catgaacatg
      841 aaccagtaca gggagttcct ttcccgcttc gtgaacaaag gctggaacga atcggaactg
      901 aaccgttttt acaaggctcc gcaacaatta tacgcagccc agttctacat acccagaact
      961 cccgccaaag acgctcccaa ggcctacggt tgcgatgaca aagtaaagcc tagccagtgg
     1021 ctcgccattt accggggaaa agtacgcgcc cccaagtccg gcacgttccg attcgtggga
     1081 ctgggagacg actacttagt agtacggttc aataaacaga atgtcttcga ctacggctgg
     1141 gaatccgctt ctctgggaaa aatgacggca aacaatgcca aatggcttga tgccatggaa
     1201 ggcaaacccg gcaatgacga cttgaaaaag gaactccggg aagtgggcat caatgtcccc
     1261 cccgttacgt tctacaaata cagttcttcc gggcactgga acaacactat gcgcggtgtg
     1321 gcagccggca agcagttcac ggtggaacag ggcaaggttt accccattga aatcttggtt
     1381 agtgaaggtc ctggtggtga attcggcatg actcttctgc ttgaagaagt tggaatggcc
     1441 cccatgagca aggatcctaa aacgggagct cccatcctgc ccctgttccg gaccaactac
     1501 ggcgttccga aaccggacaa gaacaaggaa catgtgccat ttgacgaaat cggcattgtc
     1561 tgggaatcca tcaaataaca ggcaattctc cgtcttcctc tctccatgcc cacaggaaac
     1621 cctgtgggca ttttgcttgc ccgtccataa aagatatcct acgcttccgc tacaaccatg
     1681 cagccttttc gattccattc catctaccag ccgcgcatct ggggcggcca gagcatgaga
     1741 accatgctgg gacgggaact gcccgaccgg gaaacagctt atggagaagc gtgggaaatc
     1801 agcgaccgtc ctgaagccat gagtatagtc aaggacggcg aatgggaagg aatgcccctg
     1861 catcggctgt gggaagaaca tcggcaggaa attttcggtc ccggctacga gcgtttcccc
     1921 cgctttcccc tgctctgcaa aattctggat gcccgcgaaa atctttccgt tcaggtgcat
     1981 cccccggaac gcacggcggc agcccggcag ggagaagtaa aaaatgaaat ctggtatgtt
     2041 ctgcatgcat ccccagacgc cctgatttac gggggaatga aggaaaacgc cacactcccg
     2101 gacatccgtc atgcggcgga aacaggacag atggagcaac tgatacgctc cacccatctg
     2161 gaaccggggg aacaccttta cattccggcg gggctggttc atgccattgg agccgggcac
     2221 ctcattgcgg aaatccagca gaatagcgat accacctacc gcctttatga ctggaaccgc
     2281 acggatgact ccggacgagg acgggaactg cacctggaac aggcattgga ttccatcggg
     2341 gaattccgga aactcagcca gcgccccgga tacctgacgg aaatgccgca tttcaccaca
     2401 aaggaatacc gcctggataa gggagaacgg tttacccagc cggactcctc ccgctttgcc
     2461 gtcttcacgg tcctctgcgg ttccgtgctc tgggacggga aaacggcccg gaagggagaa
     2521 ttcattctgt ccccggcagg cgcggatgcc gtcaccgccg cagaagcaga tactgttctg
     2581 ctctcaacaa cggtttaagc aaaccaatat gacaaaaaga gcgaagaatt cggcacaatg
     2641 ctgaagcttt cacacttttt cgttgcaaag aagaagagcg aatcttagcg tgaacttaac
     2701 ttttcctgca tccgtgctga aaattcccca tcccccggcc tccgtatgag caatatttcc
     2761 accatcgcca cttatcttgc ccgcttttcc ggtcagctcc agcccatgct ggagaaagtc
     2821 cagggcatgg accgcagaac ttttctgctg gaaaccgccg ccctgcttgc cctgaccagt
     2881 tgttcggaac cttccggaaa agcaggaatg gctcccatga tctcctttaa gccctccggg
     2941 gtccccgtgg tgaaacgttc tccgcgccaa ttgctggcgg aatgtaacgt ccggccgatg
     3001 ttcatgccta aatcttctcc gctccgccgc cgttcttccc gaatgaagcc acgcttcatc
     3061 accatgcaca acacggaaaa cccgtccgca gacgccatgc agcatgcgcg cgccctgaat
     3121 aacggagctt tgcgctgcaa ctggcattat acggtagacc cgtacgtgac catgcagcat
     3181 atccccctga atgaaacggg acgccatgcg gaccggggcg gccccggaga catgtactcc
     3241 atcggcattg aaatgtgtga gaagcgagga cagagcatcg tcaaaacctt tgaccgcgcc
     3301 gccaagctgg cggcctacaa catgtatgcc cacgatattc ccctgcggaa tgtcgtgccc
     3361 cattactact ggaccggcaa gcgttgcccc caccttctgc tggacaatgg caaacccggc
     3421 ttcaaatggt cttggttcat ctcccgcgtg gattattatt accgctgcat cagctgatat
     3481 caggcaacgc ctcttccctg tgcagggaaa tgggtaatat tattatgcag ccaaaaacgg
     3541 cttttctgcc agggaacagt taaaaatttc ctgtcatttc attaaaactg cttggaaaag
     3601 gctccccaaa aaagcgccat ttccacctgc ccgttattcc tgaagcatat tccttgttta
     3661 aagccccgta ccggcttcct tttttgaaaa aaggcttcca gaaacggaac cggagcattc
     3721 gccttacggc atggccaaat cctccggcat cagaatagcc gctacggcaa aagcggcaat
     3781 gnnnnnnnnn ncgcgccaag ctgttcattg gtagtggcct tgatacccac gcggaccgga
     3841 gagattccca gagcggttcc caaagctccc ttcatctgct ccaaataagg agaaatgcgg
     3901 ggagattctg caataaggga cgagtccaca ttcacgatgc gacctccacg ctcccgaatc
     3961 agagaaacgg ctttcgccac aatatccagg gaagggatgt ccttgcaggc aggatcgccc
     4021 ggcggaaacc agtagccgat gtccggcaac ccggcagcac ccagcagtgc atccgcaata
     4081 gcgtggcata aaacatccgc gtcggaatgg ccgtccaggc cgcgggagga aggaatatgc
     4141 acgccgccca gcatcaaggg gcgaggaatc tccgcaaaac ggtgaatatc atacccgatc
     4201 ccggtcaaag cgatcatgtt tttcatacgg tactgcggca ataatgttcg ggttattctc
     4261 ccagtgcctc catgataggg atgcggccgt tccatgcggc aatggcgaac tgttccggat
     4321 tatcttcctt cggttccaaa acaaccttgc cgccggctgc ttccaccagc aaaaccccag
     4381 cggcgacatc ccagatggaa atgacgcttt ccacataggc gtcaaatctg ccgcaggcaa
     4441 tataagccag ggccagagcc gcagaaccgt tattgcgcac cttgcgtacc tgccaggcga
     4501 tcctggcgaa gcgctcaatc cccttttctt tggaaccgtc cgtcttgccg tgccccacga
     4561 ataccacggc ttccgccatg cgttcccgcc ggctgcaatg catgggatga tcattcatgt
     4621 acggaatccc gcccttttcc acatgccagc attcatccat catgggatcg taaatcacgc
     4681 ccaacaccac ttccccgcgg cggcgcaaag ctatggagac gcagaaccag ggaatcgtgt
     4741 aaaaataatt caccgtcccg tcaatggggt ctacaatcca ttcacactcg ctgttcctgt
     4801 ccccggtgta tccttcttct cccaaaacag cgtggttggg aaacacggaa agaatttcct
     4861 ccgtaatcag tttctgggaa agcttgtcca actccagctt gatatcattc tggctggctt
     4921 catccacctt cttttgctca taaaaatgct tcttcagaaa agctccggcg gatttggcgg
     4981 cctggatggc cgtggtcatt tcaagtgagt gtctcatgat gcttctacta atggaaaatt
     5041 gaacttcatt gggggccctg ctctgcagca ttttcccgct cccggcgttc ccggacagct
     5101 tttctgcggc cttcctccgc acggtcatac acctccccgg ccagggagga acaggttttt
     5161 gccaacgccg cggaaacccc tttcttttca tagacggcgg cctccggaga atcaaagccc
     5221 acccaggcaa aaaccgtgaa ataacgcccc atgacggcgg aaaaatgtcc cgtatttccc
     5281 ggaagtttga cattgacgaa ggttttcctg gttcgttcgt cgtagcggaa gggaggaagg
     5341 gacgccacca ggtgagcgga ttccctaggc aggatttccc ggcggctttc ttccggagag
     5401 ggagcattca ccatcaccag gttctttttg gtggtgccca cctgcctgac ggaggaaata
     5461 ctcacattga gcccgtcatt gccgatgaga tacagggcat tgacggcatg cgaaagaggc
     5521 acggaaaacc tgccggtata caagtccgaa gaacggggaa gttccccttt gtaaccggcc
     5581 ttttgtgccg tttctattac tgtattgaaa cctaccccgc ggccagtgac ttccggattg
     5641 ctgcgaataa cggttctacg ctggtcgacg gcgcataaat tgacgatggg agtgaacaga
     5701 tcgccgggca ttaccctgga ctgccatcgg tccagaccgt cctgggcgga gcgtccccct
     5761 gttacggcca gcaactcccc tttacgggaa tccacacaca atatggccgc ttgcacacaa
     5821 cgattcaact ggttgtccac ccttttgggc aaccctgccc acacagaaga agattccaga
     5881 gcggccagca taggctcgct tacatcttca accatccgct ggaattccaa atccagcgtg
     5941 gtcattacaa aaatgctgga ggttccctcc tgctcgtcgc agcaatccag gtctcccaac
     6001 tcgcgggaaa cgaccagaac gggataagaa ccgggccgag cttcccgttt accggccacc
     6061 gccatcggtt cgcggagcgc ctgccataat tgttcctggg taatgaattc acactcaaac
     6121 atcctttcca gcgtcgcatt gcggaccttg gcagcagcct ccgggctgta aacaggattg
     6181 tagatggaag gtccgcgcac cagaccggcc agggtagcgc actccgccag cgtcagatcc
     6241 gccacttttt tgccgaaata caaatccgcc gcctgcgcaa tcccatagca ttgctggcca
     6301 aaataaatgc ggttcaggta agccgtcaga atttcatcct tggaatactt gccctcaatg
     6361 cggcgggcca cggccatttc caaaaccttg cgatccaggg tcttcccccc cagctcataa
     6421 caattccgcg ccagctgcat cgtaatcgtg gaagcaccct gggcatagga caacgtagtc
     6481 agattccggc aaatggagcg gataatggaa gagtacacaa tgccgccatg ttcgaaaaag
     6541 tcttcatctt cccgcgcaat gaaagcattc accagattat cgggcagttc attgcgcgct
     6601 acatacaccc ggtcatgatc tgtcaaggtg cctatccact ggccgttgcg atcatatgcg
     6661 gcacaatttt ccaacggcgc caaaacctcg tccagctcat actggtccgc cctccatgca
     6721 taaacgagaa gaacgatgat gaaagcgata ggaatgaggc tgaaccacaa caacagacgg
     6781 cccagattgc gaggcagcaa tccaaacaga tcataccatc ttttacgcct gttttccatt
     6841 tccgggttca atccttaaac gcttatcgtg cgtccggagg caaagactct ccatcctgtt
     6901 tccaatccgg gaattccttt tgcaggcggc cccgatattg ggaagcctcc gatttgcggc
     6961 cggccttgtc caaagcttcc gccgccttga acagagcctt gggtttcagt tcggcgtcat
     7021 tgacaaacag ctcggccgta acaccgtaat gcttggccgc ttcatcaaac tttttctccg
     7081 cataggaaat atcccccaga acaattttca aggaagccat cagaggacct tccacgccca
     7141 gagccagcgc atcttccgcc gtcttcctgg catccgcata cttgcccagc cccagcaaaa
     7201 tggaagcctt gtccagcatg ccgtccgcac ggcggtaagg ctggtcctca tccttcagga
     7261 aattgttgga agccaccaag gcgcggtcat actttctgag cgccaggcgc accttcgcca
     7321 aattacgcca aatcaccttc ttcacattct gaagctggtc gttctgcgta gcccaggtca
     7381 tatacttgtc cgccgtttcc agatccttca tcccataggc ctgaaggccc agccatgcgg
     7441 ggatgacatc cggcacacga gccacggcgg aaggattttc cttttgcagg gtttccaggg
     7501 cttccttcag tttattcacg tcctggagct tgtgatagga cagaacggaa agcacattaa
     7561 cctgttctcc atatttctgg ggatccagag tcttggcctc cgccatatgg ctgatagaag
     7621 acttgaaatc tccctggtca aacgccgcac gccccagcag gaaatgagct tccgccagag
     7681 cggcggggga cgccttcggg aaattctgaa tcaggccttc ataatacttc gccatgttcg
     7741 ccatatcctg gcgtcccgca taaatctgag cggctctctg ccatgcggcg gcagctgatt
     7801 cagctttcgg ccaacgggca atcacacggt caaaatccat cagagcctca gcttccttct
     7861 tggtcttggc atacatgtct ccgcgtaacg tcaatgcctc gcaaatgcgg ggatcttttg
     7921 gaaaatcatt gataaaatca gtcagcagct tggcggccac acctctgttc ccggcctggg
     7981 caatcgccca cgcgctctta tataaaatat ccgcacgcat cttcgggggc accttgtcaa
     8041 aatcaatgct ggcataaaac cgggcggcat ccgccggatt ggagctgaac aggttctctg
     8101 cagccatcag gcgcaccata tcatgcagct cactggtagg aaaagccttg gcataatggt
     8161 tcaggaagct ctgggcccgc tgggggagat ctttctgctt aagttcatta taacacaata
     8221 accggtaaaa ggaggcttgc atggcctcct gcgtataagg aacggacttt tccgcttcca
     8281 gaaagaaatc tgccgctttc cggtactcct tcaatttata ggcggactgc cccagcagca
     8341 tcaacagacg cacctggccg tcctttgtcg gcatctttat tcccttctgc tgctcatact
     8401 gggaaagtat ctccctgtac ttgcccatat tgtacagccc cagaagaagc cccattcggg
     8461 cctccggcgc catctccttc agggactcgt ccttcaaaag ccgttcataa aggccctggg
     8521 cttccgcagt ctttttcagt ttggtggcca gcatggcggc ctgaagagtg gcgattcccc
     8581 tgttcctgtt atccacaccg ggcgtttcca gcaccttgcg atattcggaa taagcttctc
     8641 ccaagcgtcc cgtatccgca cacagggcgg catatcccat ccgggacacc agaacaaact
     8701 tggcatccgc ttccggattg cccatcaccg cacaaaacat ggtggcggct gcctccggat
     8761 taccgctgga aagaaacagg cgtcccagac ggtactgggc gtccaccttc aaatcattct
     8821 gttccgcatt gcgaatcacg agctggtaat actggatggc cttatctatc tccccggcag
     8881 aagaagcatc cgtcgccaga cggtaggcgg aggcggcaac cagggcgggg ctgcccgtct
     8941 gaatcaatct gaggaacagg ccatgtgcct catctttcct gcccagctcc tccaaacaca
     9001 tggcagcacg atactccgcc agaggagcct gggcggactg ggggaaacgt tgggtaaatt
     9061 ccagatattt acgcaagctt aaatcaagaa ggcgtccata ttcctgccgg ttctccttcg
     9121 tagccggagc ctgggcctgc ttgtacagca tgtccgccat atccagcgta tcctgctccg
     9181 gattagcgac cagcggacca tccgcgatgg gagcggcatc cggaatattt tcctgggcgg
     9241 cggaaaacat cgccaaagtt attaaacctg cattaatgga aaaaaacgct ttcatgaagc
     9301 ctgggaattg gaaacattga tggaagatgg agccggagga cgcgtggaaa aagaaacgtt
     9361 attcagcccg gccttcagac atgcgtccat cacatgcacc accacgccgg attccgcctt
     9421 ctcatccacg cggatacgga cgagcatggt tttgtccaac cgggcgacgg aaaaaagttt
     9481 gtcttccagc tgcggcattg tgtactgctg gcggttgatt accacggtac catctttacg
     9541 gatattcacg ataatttcat tggacacgtt ttcaccgggc ttgggagtct ctgctgaagg
     9601 aagattgatg gaaagatccg gttcatcatc ctcaaatgtc tgggttacga tgaaaaacac
     9661 cagcagcaga aatacgatgt ccagcatggg ggccaattga aacccgatgg gactgggcat
     9721 tttataatga aatttcatac attcttccgg tgaggtggag aagtggatac gttccggcta
     9781 gagcccacgc acatcgcgca gggaagtggg atctacgggg actttgggaa gttcctcttc
     9841 atgaaaagcc ccagctgcac cgactcgtgg cttctgaagc tgggtggcaa ctacggaaac
     9901 actgtgcgtg acggctgcct ccaggtctcc cacccgtttg tgtacacggc tgcggaaata
     9961 tacataggcc aggatggcgg gaatgccaag catcaggccg cccgccgtcg taatcaaggc
    10021 ttccgccacg ccgcccgcca tgtcgatgcg ctgcttgccc ccggaaaaat caccgttggc
    10081 aatctcaaaa aaggttttca tcatcccgac cacggttccc aacagaccca gcatgggggc
    10141 tacggcgcca atatccgaca gccaggaaat ctggcggctt aggaaccccg cctgccgtcc
    10201 tccttccgca gaggctatct cccttacctc ctcaaactgg gcggagggat tgcgcaacat
    10261 gaagttggct acagtcaaca tcacgcgagc gtagcaggaa tctccattct tgcagaggat
    10321 ggaaagtccc gtgtaatccc gtttgcgaat gaaagattcc gcctgttcca ccagtttggt
    10381 aggcaggacg gcggaagctc gggtagaaaa acagcacatg accagcacca ctactccggc
    10441 gatggataac agaagcagcg gccacatgat gaaaccgcct ttcatgaaca gctcagaaag
    10501 gggataattt ctatctccca ccacaaaatc catggcggcg gccaacgtgg taaacggtac
    10561 agggttcata caaaagacat aggggattat acagaagaag ttttcctagg caagccaagt
    10621 agccgtaata cagtacgttt ccgcagttcc atttcttgcc tcatatggca taatgcctgc
    10681 gtggcagacc gcgagcaacc aaccctgaaa gaactctgga gggaaactcc gcacaaacca
    10741 ggcgtttata tcatgaaaga cgcccgggga aataccatct acgtgggcaa agccaaggac
    10801 cttcaccgcc gcctgggcaa ttatttttct cctacgggag ccacactctc caatcataag
    10861 acaagagcgc tcattaatgc tattgcgtca tttgattatt ttgaaaccag aaatgaccag
    10921 gaggcttttc tgctggaaag caaactgatt aaacaatatc gcccgcatta caatatccag
    10981 atgaaggacg acaagcgcta ccccctgctg aaaattccga agggggaaaa actgcctcgt
    11041 ttccagctgg cgcgggtgcg caaagatgac ggagcgcgct acttcggccc ctttgtccac
    11101 tcccaggctc tttacgccac gcaggaatgg ctcaaccggc atttccggct gcgaacctgc
    11161 aagacaaaaa atccgggtat ccatgacttc aggcactgcc atgcggatgt gattcgcaac
    11221 tgctccgctc cgtgcgttgg ccgcatttcc atcaatgatt acaaccggaa ctttgaccag
    11281 gcggtgcgtc tgctggaagg gacgggaaaa aaaagcgctc tggacgaact taccagggaa
    11341 atgatggaag ccgccgatga actggacttt gaacgcgcgg catatctgcg ggacatccgg
    11401 gacaatctgg tcaaggtact ggaacccgcc cggaggttcc gaaaaggaac tcccgatctt
    11461 cccggcaccg ttcacccgga agaagacatg aaggaactgg gggtagcact gggattagaa
    11521 tctcccccac tcattatgga atgctttgac atttccaatg tctcttccaa ccatatcgtg
    11581 gcctccatgg tgcgcttcac caacggcagg ccggacaaca aggcctaccg gcggtaccgt
    11641 atacgcactg tagacggaca aaatgacttc gcctccatgt cggaagtcat ccggaggcgt
    11701 tactcccgca ttctggcgga gagcgatgcc gtagcctcac ggcaggcgga catgaccctg
    11761 taccagtggc ttaagaaact cagcgcggaa ggaaaagccc ccatcaaagt tccagatctg
    11821 gtagtagtgg acggaggtaa aggacagctt tcctccgctc tggccgattt ggaagccatc
    11881 ggcctgggag acatgcccat cgtaggcctg gccaagcaga gggaagaaat atttttcccc
    11941 caccagtccc agcccctttg cctgcctcac agcacgggag ccctcaaact catgcagcgc
    12001 atccgcgacg aagcccaccg cttcgccaac ggctataacg aactgctcta ccgtaaacgc
    12061 atgcgggaaa gcgccttgga cgacgcaccg ggcatgagcg cctcaaaaaa aagactgttg
    12121 ctggaaaaat tcaaatccgt aactgccatt aaaaaggcgg acccggcttc tatcgccgcc
    12181 atccgcggca tttcggaaac ttgggcccgc accctgctga actaccttaa ctcatcttcc
    12241 aactcctgac tcctcatgca attcctgatt ctggccgccg gaaagccttc tctaggctac
    12301 gccaaggaag gcgtggaact ctatctcaac cgtctcaggc ccttcggcaa aacggaactc
    12361 aaacttgtca gggatggaaa ctcccaagac gtttctaaac gtctgctggc cgccagcgag
    12421 ggttgcctgc gcatcgccat ggacgaacgc ggagaacttt ggaccacaaa aaagctggtc
    12481 gacctggcaa aggactggca aatgcattcc gtgcgccgca tcgcgtttct catcggagcc
    12541 tcagacggtc atacggaaga attgcggtcc cggtgcaatc atatcctgtc cttgagcaaa
    12601 ttcacccttc aacatgaact ggcgcttgtt gtcctgctgg aacagcttta ccgctgtcac
    12661 actattctgg caggaacacc ataccatcga taaccctcgt caaccaaaca ttcctgtata
    12721 ggaaaacctg ttcccttcac ggcaaatgca ggaaaatcat tgaaagccgc cgtaagcaat
    12781 ccaaccagaa aaagcttttt aaagacgcgt gaagcagtaa aaaacagagc tcttcctgca
    12841 acaaaaaagg agcactccac agggagtact ccttttccga gaaagaaatg gtaaagccag
    12901 ttccttcgca accagccaag cttaacgctt ggagaactga taacgcttgc gggctccagg
    12961 acggccgggc tttttacgtt ccttcatacg ggaatcacgg gtcagaagac cgaattcacg
    13021 aagctgggaa cgggaagctt catcaacctg aacaagagca cgggcgatcg ccatgcgaat
    13081 ggctcccacc tgtccgtgaa taccgccgcc cttggtaacg acgttcacat caaacttgtt
    13141 catggtattg gtaacctgaa agggctgaag aacagcattc tgcaactgaa cggtaggaag
    13201 atattcctca aaaccgcggc ggttgatcgt aatgcgaccg gaaccttcag taagccaagc
    13261 gtgtgcaatg gcggtcttac gacggccggt ggcgttgtat ggggttgtct gactcatgtc
    13321 tgaaaaatca attagagagt aatggcagtg ggctgctggg cttcatgcgg atggcaggcg
    13381 ccggcataaa ccttcagctt ggtcatttgc tgacggccca gacgggtgtg aggaaccatg
    13441 cctttaacag ccagttccag aagaagttca gggcggcgag cacgaatctt acggggggtg
    13501 tccacctgct taccgccaac gtaaccggag aaacgggtat aaattttggc attttccttg
    13561 ttaccggtca acaccacctt gtccgcgttc acgataatga caaaatcgcc acaatcaacg
    13621 tggggggtga aaattgtctt gttcttgcca cgcaaaatgt tagccgcttc aacggcaaca
    13681 cggccgagca ccttgtcggc agcgtctata acataccatt tgcgctctac ttcttgcggt
    13741 ttggctgaga aggtcttcat ggttcaattc ttcgttctcg tttttcggca tcctgccgtt
    13801 atcttccaat tcgggggcgc attcctaatg attattcacg taaatgtcaa atactttttc
    13861 tcttttacag cctttttccc gtccctctcc atagaattgt gttttatttg cacaacttcc
    13921 gcattcagga cttgcccttg caaaaatatg gtctatcatt cgtctgcgcg ggatttgtac
    13981 acacgcgttc attttaactt tacaaggaga gtcctatcag cccgaatcca agatccagca
    14041 gccgccccag cggcggacaa aattcccgcc ccggttccag accgaccggc accagtaacc
    14101 gtcccggaca atccagacct tccggcaatg gcacaggagc ggcacggtcg gccggacagt
    14161 ctgcaggacc gggaaattcc agaacagggg gaggaagccg ccccggaggt aacgccaaca
    14221 ggcaacgccc gcaaggaggc ggaggacgcc acaccaaggg cagaaagaat ttcaataaat
    14281 cagagtccaa ggaagaaaac tccgaaagtg aaattgaagt ggaaggcacc atctgcgccg
    14341 tactggcagg aaccatgttc aaggtgcgcc ttcccaacgg gcacgaagtg ctggcccata
    14401 tttccggcaa aatgcgcaaa
//
LOCUS       DAOMSU010000027        13880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_187_length_13880_cov_2.438192, whole genome shotgun sequence.
ACCESSION   DAOMSU010000027 DAOMSU010000000
VERSION     DAOMSU010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13880
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_187_length_13880_cov_2.438192"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..288)
                     /locus_tag="PK077_02270"
                     /pseudo
     CDS             complement(1..288)
                     /locus_tag="PK077_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
     gene            complement(392..901)
                     /locus_tag="PK077_02275"
     CDS             complement(392..901)
                     /locus_tag="PK077_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRN23156.1"
                     /translation="MKIGIFSDLHNRTDHLEPAMHRMRLLGCGHFIFLGDCTTPESFR
                     RLIELTEDFPLDAVPGNNDYDVETMRRMAASSPAARLHPEHALITRYGMKLSLSHYPK
                     YALREARSGNADAALYGHTHQAVSERCGGCLLANPGELQGRTGRIGFGVLDTDTRIIN
                     LHNLDFYAP"
     gene            complement(908..1876)
                     /locus_tag="PK077_02280"
     CDS             complement(908..1876)
                     /locus_tag="PK077_02280"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HRN23157.1"
                     /translation="MSSSQILRLARKLELTAEQERDFLDAMQAGNRSRSALVITPKAP
                     EGYRPPLPAKDTPQEWGHSSILALPSGETETKPGSLPDYECGYYYPLDLSSVWETAPL
                     AHLPFRPGRCLDMCAAPGGKSILAQTRVSPQEHVSNEVHPKRLGILRHNLLRCGFTNL
                     YTQRLRPDQWAELAPGCFDLILADAPCSGQSLLAKGIPNPGCFNSSVTGGNAKRQRGI
                     LLAALRCLAPGGFLLYTTCTYAPEENERNVLYLLKRHPDLHTVTVPELEHFRSSLTQE
                     ACYRLMPFHGAGAGGFTCLLRKEGGHPLPLSLPDELKAWPIHAMNC"
     gene            complement(1881..>2489)
                     /locus_tag="PK077_02285"
     CDS             complement(1881..>2489)
                     /locus_tag="PK077_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRN23158.1"
                     /translation="AYAEWKLGAGKGKRHLVCLTLGTGVGSGLIVNGELLRGSTCSAG
                     ELGQTSIDYRGRLGHYGNRGSLEDYVGNREIAADARTLYASHGIDKAIVDCNPIALER
                     AALAGDEVAEQVWRDLAVKLSCALMNCCYLLNPEAIIIGGGVAKAKTLLFQPLQEIMK
                     AQLAAPLVEYLEILPAQFGTEAGILGAAHLALNTHFGETFRA"
     assembly_gap    2490..2499
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2500..3032)
                     /locus_tag="PK077_02290"
     CDS             complement(<2500..3032)
                     /locus_tag="PK077_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRN23159.1"
                     /translation="MTASTIPSIGIDFGGTSIKMGVVKGAEVIAHAPSIATQEYGNPD
                     QLIEAIAQFVNMLRLNHPEVQAIGMGMPGFVNFYQGTVYTLTNVPGWNNVPVRDMLQA
                     ACGLPVYVENDANCAALAEYHAGAGQGSKRFVTITLGTGIGGGLVVGGKLYTGFAQSG
                     LEVGHMIIRPNGVLCGCG"
     gene            3268..4092
                     /locus_tag="PK077_02295"
     CDS             3268..4092
                     /locus_tag="PK077_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HRN23160.1"
                     /translation="MPTPKKTNSKTVAKEVPASKKKTCGKSDCKTAVDLEAPAKKKCC
                     RKKASEPEKAAKPAKEAAAAAAEMEKKPAPKTARKAPVKKTAPAPVAPVPAPAKNELT
                     DEQAEAIAAARAELAANPEWEPFVQMQRQHLLDLRDRALDNMSGVARDTLRNHPEGSE
                     ASGSGEHQADAGSDAYDRDFALSLLSKEQDGLYEIEQALARIDNGTYGICEMSYKVIP
                     ILRLEAIPFARLTVECQAQWEKEKGQNARFRPRVALGFAGGQNDVDLSVSLDDDEE"
     gene            4126..4299
                     /gene="rpmG"
                     /locus_tag="PK077_02300"
     CDS             4126..4299
                     /gene="rpmG"
                     /locus_tag="PK077_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRN23161.1"
                     /translation="MPRDIIILECTEAKAEGKPTSRYVTTRNKKSLRTPGRLEKIKYN
                     PFLKRRTLHREMR"
     gene            4331..4576
                     /gene="rpsR"
                     /locus_tag="PK077_02305"
     CDS             4331..4576
                     /gene="rpsR"
                     /locus_tag="PK077_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRN23162.1"
                     /translation="MSTEPKTVERRIRFRTCNRQMPRRRLDIPMDQVNLLNPDFLSKF
                     TSETGKILPRRVTGLSAKMHRKITREIKRARSINLLP"
     gene            4684..5478
                     /gene="folE2"
                     /locus_tag="PK077_02310"
     CDS             4684..5478
                     /gene="folE2"
                     /locus_tag="PK077_02310"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HRN23163.1"
                     /translation="MQELKDTQSEADTRNISIDRVGVKGLRFPIQIQDKLNRIQSTVA
                     TVSLAVDLPEEFKGTHMSRFVEALHQHGPLLDVHTALAIPRELLRRLSARRSHVEMEF
                     PFFRSKNAPVTGIEGLMDYVVRFEMEAEANNKLADFKLTVIVPVTTLCPCSKAMSAYG
                     AHNQRGLVTYSVRFASRPVWIEDLIDLVEFCASCSLFSVLKRPDEKWVTEKAYENPVF
                     VEDLVRNVALKTQSHSAFSWYRVEAENFESIHNHQAYAVIERDLRS"
     gene            5499..6257
                     /gene="ccsA"
                     /locus_tag="PK077_02315"
     CDS             5499..6257
                     /gene="ccsA"
                     /locus_tag="PK077_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRN23164.1"
                     /translation="MNDNWWAVASLILSLAFTGLGWRLLASGRRFLYAHLWMACLFVL
                     QTGALCVKAYQTGMCPIRGASEVLFFLSWSINLFYLMLGRAYRMSVLGIFTAPAIAVL
                     TVFSLLIGRSGADVQGTHDFWVTAHVGIAMMSYGAGGLAAAAGAAFCMQNECLKRHRI
                     PGTCRVLPPIRTLEASMKRLVLVAFLLLLLGEWFGWRGHLPINAAKASIVILLTAGYS
                     VLLWFIYRRGIPGRMLACCCVALFIASMSIFLVS"
     gene            6278..7279
                     /gene="hemA"
                     /locus_tag="PK077_02320"
     CDS             6278..7279
                     /gene="hemA"
                     /locus_tag="PK077_02320"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HRN23165.1"
                     /translation="MNHRTAPVEIRERFAVPSHKLREEGQRIRSLPDVDQCVVLSTCN
                     RMEIYYWSNEPENAQEHILSHFLGDGRGELDMASYFYSHQGEDALGHLCRVLSGLDSM
                     VLGETEIFGQVKTAYHTALDAGITAACANKTFQKAFTIGKKVRTESQIHAGATSVGSV
                     AVELAEQIFGDLSGTRVLILGAGEMSRVTGRALHARGAEGIYVANRSFDRAVELAGMI
                     GGQAIRYDVWGNYLRDIDVVVAATAAPHCIITRETLLPLRASRKYRSLFLIDISVPRN
                     ISPDVADIDEVYLYDIDTLTQLADEAKRSREQEVSRCEAIIRDSISRYFPDSALYDQ"
     gene            7269..8246
                     /gene="hemC"
                     /locus_tag="PK077_02325"
     CDS             7269..8246
                     /gene="hemC"
                     /locus_tag="PK077_02325"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HRN23166.1"
                     /translation="MTSKNTLIIGTRGSALALAQADMVRAALSLRYPELDVRCEIIHT
                     IGDRRTDVPLADVARVSGMVDKGIFIKELETALRDGRIDVAVHSLKDVPSELASGFTL
                     AAVLPRAAVEDVLITKEPDWNGSGTLATGSVRRRLMARTYWGSGLRFENLRGNVPTRL
                     GKLVEHPGWDAVILARAGLERLGLYAPETLVEGRKLYMRPLPVEVFIPAVGQGIVGME
                     CRSSDRETAEMLEGINDPESFACALAERAFLVRLGANCSTPVGVYAHPDGEELVLRAA
                     YYVPGREEPFAVVIRGDRKAPEALGLRAFEELGLSGWRKTSSCSGCGEK"
     gene            complement(8299..8493)
                     /locus_tag="PK077_02330"
     CDS             complement(8299..8493)
                     /locus_tag="PK077_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23167.1"
                     /translation="MNKRRKWKGGEYNMASSSHMGGSHKGSSKKRSETTTSKTGRRIK
                     RDASTGQFMSDKSSGSKEKH"
     gene            complement(8583..8939)
                     /locus_tag="PK077_02335"
     CDS             complement(8583..8939)
                     /locus_tag="PK077_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRN23168.1"
                     /translation="MNITIKRIYDPKEPGDGYRVLVDRLWPRGISKEKASWDEWLKNI
                     APSTALRQWFAHDPVRWDAFRKKYDEELDGNGEAVSHLLRLARKGKVTLLYSARNTEH
                     NEAAALRDYLLQRFAS"
     gene            complement(9337..11289)
                     /locus_tag="PK077_02340"
     CDS             complement(9337..11289)
                     /locus_tag="PK077_02340"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRN23169.1"
                     /translation="MPDSPPSTSPAPRIAETAAGKILAGLLALFYIFLTTTAAGGEWH
                     TLFLPACFLAAALLLFCFCILRGYKIPSPGLPGWLALGLGGGYFLVRAWFSPWFYYES
                     VADLGLIATAIVMFAAGTYAGAGNGEKSILPVLAAALGLLNALLWGYQNITGTEASWF
                     RPDYSLFGTEIRNIGLFGYKNFSAHFLSVTGFFLCAYSMASARKWGIRLFTGLALILV
                     SFTCGSRSAFPNALAGVTLCFFIYTSSVFRNNRKFYTASILFIILLFLGTSYAVLDLS
                     RGAGRLTALLDTFSFGNRLDLSKLAWALADQAPLFGHGSRMYTNLSTEFFSGANLPNF
                     AHHEYAQAACDYGYAGLGLMLALLALFLISGLRSVLKLSGEHQRPNPLGPAAFCVLCI
                     AAFHAYGEFIWHNPALLGASALCGGITCTAPLSRVKASRRVGRWLQATAALLMAILAL
                     CYAFLAFPVWKNSLQAVPASSSNRLPMLEAAASCSLDPDLVRRNILHAAGSSPPPSPA
                     RLKVLEHQEEKAERLSPGNHGLTAAKSLLYILQGRLTEAEQLLRPYVESPGRFDDRMF
                     AWTTIYNNMLYSWSTAIAAQSPGRALSMAMTAQRLMSAQTDRWLYYGALDPEVRKKHY
                     SRLNELKMLIMMLQARRTTPDPSWRK"
     gene            complement(11619..13520)
                     /gene="mutL"
                     /locus_tag="PK077_02345"
     CDS             complement(11619..13520)
                     /gene="mutL"
                     /locus_tag="PK077_02345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRN23170.1"
                     /translation="MPSIHVMSPTLASQVAAGEVVERPASVVKELVENSLDAGAKFVR
                     VEIRRGGVGMIKVTDDGSGMSRADAELCTKRHATSKLSSLEELFEITHLGFRGEALPS
                     IASVSRFKLCTRQQQDLEGWEIRIDGGLEHEPRSSGVSPGTAIEVADLFYNTPARRKF
                     LKSAETEASHVEHQIRLHALAYPQVRFAYKRDDQLVFDLPATADLRVRISALTDAATA
                     AALIPIETTIGPGISITGFLLPLSEARRTRKGQYVFMNTRPVEDQLINRAIRDGYGGF
                     PTGLHPALFLYMEVEPALVDVNVHPAKKEVRFRRSADVVNTIVEAIANTLQKHARQEI
                     HAAAAPEPERTLPPAHSTTAPYGEIPARSTNPGSAFPAAARPAPASSAAQPPLSSSAR
                     QSHGPVPPPTLRAIPLKQVPATQGKLDFHRQEDEETARNAHENAASERDASAGFSYLG
                     TLRQQFALFETPEGLVLMHPKAARERIIFERLRARREAPMPSQQLLDPVVLDLDPRDF
                     AVIRQFAPHFDQAGMVVTPFGQNTIRIESIPALLELENARAFLLELVDRLTQSEFSRN
                     AKRVAYETFIGEFARKSAWRERISPHRAPAILKDLLACEVPYCTPGGKPTLVNYSVPE
                     IKRKFGLQA"
     gene            complement(13563..13775)
                     /gene="thiS"
                     /locus_tag="PK077_02350"
     CDS             complement(13563..13775)
                     /gene="thiS"
                     /locus_tag="PK077_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HRN23171.1"
                     /translation="MAAQNDIMLNGSPYELAEPCSVSRLLELLNMKGKPVVVEHNGAA
                     LLPQDFSRVTVSPGDRVEIVSIVAGG"
BASE COUNT         2913 a         3769 c         4205 g         2983 t
ORIGIN      
        1 ttccagccat tcctttaaca caaggcggtc cgcaggcgcc cagggcagtt cttccagcgt
       61 acggcgggag aagaacccca ggttttcatg ttccagaggc cggggcaggg cgcaaagttc
      121 cagacggcac agaaagggga taaggcggat aatgcgttca ggctcccggt gccgtacggg
      181 ggtgagcatg cggacgggga agacggtgca tcccagctct tcccgtattt cccgtatgac
      241 ggcgtgccgg gcattctccc ctggttccac ttttccgccg gggaattcgt aaagaagccc
      301 gttggactgc ccgttttctt tttagcgccc agaagaagag ggccccgtgc ggcgggcagt
      361 tcaatcaggg cacagcagac gtccagcgtg ttcatggggc gtaaaagtcc aggttatgca
      421 ggttgatgat gcgggtgtcc gtatccagga cgccgaaacc gatgcgtccg gtcctgccct
      481 ggagttctcc gggattggcc agcaggcagc cgccgcatct ttcgctgacg gcctggtggg
      541 tatggccgta cagggcggcg tctgcgtttc cgctgcgcgc ttcccgcaag gcgtattttg
      601 ggtaatggga gagggaaagt ttcatgccgt accgggtgat aagggcatgt tccggatgca
      661 ggcgcgccgc tggggaactg gcggccatcc gccgcatggt ttccacatca tagtcattgt
      721 tgcccggaac ggcatccaga ggaaagtcct ccgtcagttc aatcaggcgc cggaaactct
      781 ccggggtggt gcagtcgccc aggaaaatga aatgtccgca gcccagcagg cgcatccggt
      841 gcatggctgg ctccagatgg tctgtgcggt tgtgcaagtc ggaaaaaatg cctatcttca
      901 tcttttttca acaattcatg gcatgaatgg gccatgcctt caattcatcc ggcagggaaa
      961 gcgggagcgg atggcctccc tccttgcgca gcaggcaggt gaaaccgccc gctcctgctc
     1021 cgtggaaggg catgagccgg tagcaggctt cctgcgtcag ggaggaacgg aaatgctcca
     1081 gctccggcac ggttacggtg tgcaggtccg gatggcgctt cagcaggtac aggacattgc
     1141 gttcattttc ctccggagcg taggtgcatg tagtgtacag caggaagccg ccgggagcca
     1201 gacaccggag ggcggccagc aaaatgcccc gctgccgctt ggcgttgcct ccggtgacgg
     1261 aggaattgaa acagccgggg ttgggaatgc ctttagccag cagggactgc ccgctgcacg
     1321 gggcatccgc cagaatgagg tcaaaacacc cgggggccag ttccgcccac tggtccgggc
     1381 gtaggcgctg ggtgtacaga ttggtgaagc cgcagcgaag caggttgtgg cgcagaatgc
     1441 ccaggcgctt cggatgcacc tcattggaaa catgttcctg cggagatacc cgcgtttgcg
     1501 ccagaatgct ttttcctccc ggcgccgcgc acatgtccag gcagcgtccc ggacgaaacg
     1561 gaagatgcgc cagaggggcc gtttcccaga cggacgagag atccagtgga tagtaatagc
     1621 cgcattcgta atccggcagg gagccgggtt tggtttctgt ctctccggac gggagcgcca
     1681 agatggagga atgcccccac tcctgcggcg tgtcctttgc cggcaagggc ggcctgtagc
     1741 cttccggtgc cttcggggtg atgaccagcg cgcttctgga acggttcccg gcctgcatgg
     1801 cgtccagaaa atcccgttcc tgttccgccg tcaattccag tttgcgggcg agtctgagta
     1861 tttgggatga agacacgaga tcaggcccgg aatgtttctc cgaagtgggt gttgagagcc
     1921 agatgggcgg cccccaggat gcccgcctcc gtaccgaact gggcgggaag gatttcaagg
     1981 tattccacca gaggggcggc gagctgggct ttcatgattt cctgcagggg ctggaaaagc
     2041 agggttttgg ccttggccac gcccccgccg atgatgatgg cttccgggtt caggagatag
     2101 cagcaattca tcagggcgca ggagagtttt acggccaggt cccgccatac ttgttcagcc
     2161 acttcatcac ccgccaatgc ggcccgttcc aaagcgatgg ggttgcagtc cacaatggcc
     2221 ttgtcaatgc cgtggctggc gtaaagcgtg cgtgcatccg cagctatttc ccggttgcct
     2281 acataatcct ccagggagcc gcggttgccg taatggccca ggcgcccccg gtagtcgatg
     2341 ctcgtctgcc ccagctcccc ggcggagcag gtagacccgc gcagaagttc tccgttcacg
     2401 atcaggccgc tgccaacgcc ggtgccgagg gtcaggcaga ccagatgtct ttttcctttt
     2461 ccggcgccca gcttccattc cgcataagcn nnnnnnnnnc cgcagccgca cagcacgccg
     2521 ttgggccgga tgatcatgtg gcccacctcc agcccggact gggcgaagcc ggtgtacagc
     2581 ttgccgccca ccaccaggcc gcctccgatg ccggtgccca gggtgatggt gacaaagcgc
     2641 ttgctgccct ggcccgctcc ggcgtgatat tccgccaggg cggcgcagtt ggcgtcgttt
     2701 tccacgtata cgggcagtcc gcaggcggcc tggagcatgt cccgtaccgg aacgttgttc
     2761 cagccgggca cgttggtaag cgtataaacg gtcccctggt aaaaattgac gaaccctggc
     2821 attcccatgc cgatggcctg cacttccggg tgattcagcc gcagcatatt cacgaactgg
     2881 gctatggctt caatcagttg atcgggattg ccgtattcct gggtggctat tgaaggagcg
     2941 tgggcgatta cttccgcgcc cttgacgacc cccatcttga tggaggtgcc tccgaagtca
     3001 atgccgatgg agggaatggt tgaagctgtc atataacgcc aatatagaat tttatcgggc
     3061 gttgaaaagg ttaaaagaag tatgttcccg gtaaaatccc gacaggaaaa tagagggtgc
     3121 aaaaggggga aaaatgactt gtttcatcac tgaattttct tgaatgaaca tttgggcgtt
     3181 tgtaatgtct catagtctag tcagcggggc gggttctgcc ctctgttcgt gaaatattgt
     3241 caacctcagc ccatagagac acctcctatg cctactccca agaaaaccaa tagcaagacc
     3301 gtcgccaagg aagttccggc ttccaagaaa aagacctgcg ggaagtccga ttgcaagacc
     3361 gctgtagacc tggaggctcc cgccaagaag aaatgctgca ggaaaaaggc gtctgaaccg
     3421 gaaaaagccg ccaagcccgc caaagaggct gccgccgccg cggctgaaat ggaaaagaag
     3481 cctgcgccga aaacggccag aaaggctccg gttaagaaaa ccgctcccgc tcctgttgcc
     3541 ccggttcccg ctcccgcgaa aaatgaattg acggatgaac aggcggaagc cattgccgcc
     3601 gccagggccg aactggccgc caaccccgaa tgggagcctt ttgtgcagat gcagcgccag
     3661 catctgctgg acttgcgcga cagggctctg gacaacatga gcggcgtggc ccgcgatact
     3721 ctccggaatc atccggaagg cagtgaggct tccggctccg gggagcacca ggcggatgcc
     3781 ggcagtgacg cctatgaccg tgattttgcg ctcagcctgc tttccaaaga gcaggacggc
     3841 ctgtatgaaa tcgaacaggc gctcgcccgc attgacaacg gaacatatgg catctgcgaa
     3901 atgtcataca aggtcattcc catcctgcgt ctggaggcca tcccctttgc gcggcttacc
     3961 gtggaatgcc aggcccagtg ggaaaaggaa aaaggccaga atgccaggtt ccgtcccaga
     4021 gtggccctgg gctttgccgg aggacaaaat gatgtagatt tatctgtttc tcttgacgat
     4081 gacgaagaat agtgtataaa tcctcccccg taacacattt tcatcatgcc aagagacatc
     4141 atcatcctcg aatgcaccga ggctaaagcc gaaggaaagc ctacgtcccg ctacgtcacc
     4201 acgcgcaata aaaaaagcct gcgtactccc ggccgcctgg aaaagattaa gtacaatcct
     4261 ttcctgaagc gccgtacgct tcatcgtgaa atgcgttaac tggttcggct tatcgattat
     4321 ctttattatt atgtccactg aacccaagac tgtagaacgc cgtattcgtt tccgcacgtg
     4381 caatcgtcag atgccgcgcc gccgtctcga catcccgatg gatcaggtca atctgctcaa
     4441 ccctgatttt ctgtccaagt tcacctctga aaccggcaag attcttcccc gccgtgtgac
     4501 cgggctgtcc gccaagatgc accgcaagat aacccgtgaa atcaagcggg cccgttccat
     4561 caaccttctg ccgtaacaag ccccaaggaa aatctctttc ggtagaattg tctttgcagg
     4621 cgggagtttg tccggtaaca gagcagactc ccgttttatt tccacccatc atcctctttt
     4681 tccatgcagg aactgaaaga tacccagtcc gaggcggata cgcgcaatat ttccattgac
     4741 cgggtgggcg taaaagggtt gcgcttcccc atccagatac aggacaagct caaccgcatc
     4801 cagtccactg tggcgacggt ttctctggcg gtggatctgc cggaagaatt caagggcacc
     4861 catatgagcc gttttgtgga agcgttgcat cagcatggcc ccttgctgga tgtgcatacg
     4921 gccctggcca ttccgcggga actgctccgc cgtctgtccg cccgccgttc ccatgtggaa
     4981 atggaattcc cgtttttccg ctccaaaaac gctcctgtga ccggaattga agggctcatg
     5041 gattacgttg tccggtttga gatggaggcg gaagcgaaca acaaactggc tgatttcaag
     5101 ctgacagtta tcgttcccgt gacgacgctg tgcccctgct ccaaggccat gagtgcctac
     5161 ggagcccaca accagcgcgg gctggttact tattccgtgc gttttgcttc caggcccgtc
     5221 tggattgaag acctgattga cctggtggaa ttctgcgcga gctgctccct gttcagcgtg
     5281 ctgaaaaggc cggatgaaaa atgggtgacg gaaaaggctt atgaaaaccc tgtctttgtg
     5341 gaagatctgg tgcgcaacgt ggcgttgaaa acgcagagtc attccgcctt cagctggtac
     5401 cgggtggaag cggaaaattt tgaatccatc cataaccacc aggcatacgc tgtcattgag
     5461 cgcgatttgc gttcctgaac catttccggg ccggatttat gaacgataac tggtgggccg
     5521 ttgcctctct catcctttct ctggctttca cagggctggg atggcgcctg ctggcttccg
     5581 gccggcggtt tttatatgcc catctttgga tggcgtgcct ctttgttctc cagacagggg
     5641 cgctatgcgt caaagcgtac cagacgggaa tgtgtcccat ccgcggcgct tcagaggtgc
     5701 tcttcttcct ctcctggagc atcaatctct tttatctgat gctgggacgt gcttaccgaa
     5761 tgtccgtgct tggcatcttt acggctccgg ctattgccgt gctgacggtt ttttccctgc
     5821 tgataggccg ttccggagcg gacgtgcagg gcacgcatga cttctgggtg acggcacatg
     5881 tgggcatagc catgatgtcc tacggagccg gcggcctggc cgctgccgcg ggggcggcgt
     5941 tctgcatgca gaatgaatgt cttaaaagac accggattcc cggcacctgc cgtgtgcttc
     6001 ctcccatccg tacactggaa gccagcatga agcgcctggt tcttgtggca ttcctccttc
     6061 ttctgctcgg ggaatggttc ggatggcggg ggcatctccc cattaatgcg gccaaggcat
     6121 ccattgtcat tcttctcacg gcaggctaca gcgtcctgct gtggttcatt taccgccgcg
     6181 gcattccggg aaggatgttg gcctgctgct gcgtggccct ctttattgca tccatgagca
     6241 ttttcctggt aagctgatga gaatggtttg tttaggcttg aatcaccgga ccgctcccgt
     6301 ggaaatacgg gagcggtttg ccgtgccgtc ccataagctc cgggaggaag gacagcgcat
     6361 ccgttccctg ccggacgtgg accagtgcgt agtgctttcc acctgcaacc gcatggaaat
     6421 ttattattgg tccaacgaac cggaaaatgc gcaggagcac attttatccc attttctggg
     6481 tgatgggcgc ggggaactgg atatggcttc ttatttttac agccatcagg gagaagatgc
     6541 tctaggccat ctatgccgcg tgctgagcgg cctggattcc atggtgctgg gggaaacgga
     6601 aatctttggc caggtaaaaa cggcctacca tacggctctg gatgccggaa taactgcggc
     6661 ctgcgccaac aaaacctttc agaaagcctt tactatcggt aaaaaagtgc ggacggaaag
     6721 ccagattcat gccggggcaa cctccgtggg atccgtggca gtagagctgg cggaacaaat
     6781 tttcggggat ctttccggca cccgcgtcct tattctgggg gcgggcgaga tgagccgcgt
     6841 tacggggcgc gcccttcatg cccggggtgc tgagggcatt tatgtggcca accgctcctt
     6901 tgaccgtgcg gtagagctgg cgggcatgat cggcggccag gccatccggt atgacgtgtg
     6961 ggggaactat ctcagggata ttgacgtggt ggtggccgcc accgctgctc cccactgcat
     7021 catcacccgg gaaacgctgc tgcctttgcg cgcctcccgt aaataccgct ccctcttttt
     7081 gattgatatt tccgtgccgc gcaacatttc tccggacgtg gcggatattg acgaagtgta
     7141 tctctacgat attgataccc tcacccagct ggcggatgaa gccaagcgca gccgagagca
     7201 ggaggtttcc cggtgcgagg ccatcatccg ggacagcatc tccagatatt ttccggattc
     7261 cgctctttat gaccagtaaa aataccctca tcatcggcac ccgcgggagc gccctggccc
     7321 tggcccaggc ggatatggtc cgtgctgccc tttccctccg ttatccggaa ctggacgtgc
     7381 gctgtgaaat catccatact attggggacc gccgcacgga tgttcctctg gcggatgtgg
     7441 cccgcgtttc cggcatggtg gacaagggga tcttcattaa ggaactggag accgccctcc
     7501 gggatggccg cattgacgta gcggtgcaca gcctcaaaga tgtgccgagc gagcttgctt
     7561 ccggcttcac actggccgct gtcttgcccc gtgctgccgt ggaggatgtg cttatcacga
     7621 aagaaccgga ctggaacggt tccggaacgc tggctaccgg aagcgtgcgc cgacgcctga
     7681 tggcgcgcac gtactggggg tccggcctgc ggtttgaaaa cctgcggggg aatgtgccta
     7741 cgcgtttggg aaagctggtg gaacatcccg gctgggatgc cgtgattctg gccagggccg
     7801 gtttggaacg cctggggctg tacgcgccgg aaacccttgt ggaaggaaga aaactttaca
     7861 tgcgtccctt gccggtggaa gtttttattc ctgctgtggg gcagggcatc gttggaatgg
     7921 aatgccgttc ctcggacagg gagacggcgg aaatgctgga aggcatcaat gatccggaat
     7981 cttttgcctg tgcgctggcg gaacgggctt tcctggtacg tctgggtgcg aactgttcca
     8041 cgcccgtggg tgtttacgcc catccggatg gagaggaact ggttctgcgg gctgcgtatt
     8101 acgttccggg cagggaagag ccttttgccg ttgtgattcg cggagaccgg aaagctcctg
     8161 aagcgctggg ccttcgggct tttgaagaat tgggcctgag tggatggcgg aagacttcct
     8221 cgtgttccgg atgcggagaa aaataaaaag gctccggacc ggatccggag cctgaatcag
     8281 gcgaaaaact cgcgggcatt aatgtttttc cttggagcct gagcttttat cgctcataaa
     8341 ctgcccggtt gaagcatcgc gtttgatgcg ccgcccggtt ttgcttgtgg tagtctcgga
     8401 gcgttttttg gaactgccct tatgacttcc acccatgtgg gaactgctgg ccatattgta
     8461 ttcacctcct ttccattttc ttcgcttgtt catgaacgag agaagaaatc ctgccgtatc
     8521 ataggttccc gtgacagcat ttggggcttg cggatggagt ttgtgcagga atatgcttct
     8581 atttatgaag caaaccgttg caaaaggtaa tccctcagag ccgcagcttc attgtgctcc
     8641 gtatttctgg cggagtaaag cagagtaacc ttccctttcc gcgccagccg tagcagatgg
     8701 gaaacggctt ctccgtttcc atccagttcc tcgtcgtact ttttgcggaa tgcgtcccat
     8761 ctgactggat catgagcaaa ccactggcgg agcgccgtgg atggagctat gttcttcagc
     8821 cactcgtccc aggaagcttt ttccttggaa atgccgcggg gccagagccg gtccaccagg
     8881 acgcggtatc cgtccccggg ttccttgggg tcataaattc gtttgatggt tatgttcata
     8941 caaaaagatt cgataaagga atagagccgg gaaagttttt ttctgttgag ctgcgtcggg
     9001 acatgcgttt tacgggaccg ccaaccgagg cagtccgcga ttgggggaaa aagaaacaaa
     9061 cgattctgtt ccggagaggc tgccgcttcc ggcggaagat agctgaatcc gcttaaaatt
     9121 ccggcgtaac gcctgtgcgg acttgctgcg ccaactatcg gcttttcatc tttctggctg
     9181 cagtacgctc tgtgttccgt ggacaaataa gctgacgtgg aatccggtaa aaaagaaacc
     9241 ggataagacg gggagacttc cgtatggagc ggcttcccgg gaaaaaggga tataacggct
     9301 gctacacaga ttccttttcc ccgattctcc ggaatactat ttcctccagg aggggtcggg
     9361 cgtcgttctg cgggcctgga gcatcatgat gagcatcttg agctcattga ggcgcgagta
     9421 gtgttttttc ctgacttcgg ggtccagggc cccataatac agccaccggt ccgtttgggc
     9481 ggacatcagg cgctgggccg tcatggccat ggacagggcg cgtcctgggg actgggcagc
     9541 aatggccgta ctccaggaat acagcatatt attgtaaatg gtagtccagg cgaacatgcg
     9601 gtcgtcaaac ctgccgggac tctctacgta cgggcggagc agctgttccg cttccgtgag
     9661 gcgtccctgg aggatgtaaa ggaggctttt agccgccgtc aggccgtggt tcccggggct
     9721 caggcgttcc gctttttctt cctgatgctc caatactttg agccgggctg ggcttggggg
     9781 gggagaactg cctgcggcat gcagaatgtt gcgccgcacc aaatccgggt ccaggctgca
     9841 ggaggcggct gcttccagca tgggcagccg attgctggag gaagccggga cggcttgaag
     9901 ggagtttttc cagaccggaa aagccagaaa cgcataacac agagccaata tggccatcag
     9961 cagtgcggcg gtcgcttgaa gccagcggcc gactcggcgc gaagccttca ccctggaaag
    10021 aggggccgtg caggtaatgc cgccgcatag ggcgctggct ccaagcaagg cagggttatg
    10081 ccagatgaat tccccatagg cgtggaaagc ggcgatgcac aatacgcaga acgccgccgg
    10141 tcccagggga ttgggccgtt gatgttctcc tgacaatttc agaacgctcc ggagtccgga
    10201 aataagaaac agggccagca gagccagcat cagccccagc ccggcatagc cgtagtcgca
    10261 ggcggcttgt gcgtattcgt ggtgggcaaa attggggagg ttggctccgg agaaaaactc
    10321 cgtggaaaga ttggtgtaca tccggctgcc gtgaccgaac agcggagcct ggtccgccag
    10381 cgcccaggcc agtttggaca ggtccagccg gttgccgaag gaaaatgtat ccagaagggc
    10441 ggtaagcctg cccgcccccc gggacaaatc cagcacggca taggacgtgc ctaggaaaag
    10501 caggataata aagaggatgg acgccgtgta aaattttctg ttattgcgga aaacgctgga
    10561 ggtataaata aagaagcaca gggttacgcc agccagggcg tttgggaagg cggaccgcga
    10621 gccgcacgtg aaggaaacga ggatgagcgc caatcctgtg aataggcgga tgccccattt
    10681 tctggcggaa gccatgctgt atgcgcaaag gaaaaaaccg gtgacggaca ggaaatgggc
    10741 ggaaaaattt ttgtatccga acaatccgat gttgcggatt tccgtgccga acagggaata
    10801 atccggtctg aaccaggagg cttccgttcc cgttatgttc tgataccccc acagcagggc
    10861 attcagcagc cccaatgccg cagccagtac gggaagaata ctcttttccc cgtttccggc
    10921 tccggcatac gttcccgccg cgaacatgac gatggccgtg gcgataaggc ccagatccgc
    10981 tacgctctca taataaaacc agggggaaaa ccatgcccgg acaaggaaat agccgcctcc
    11041 cagacccagg gccagccagc ccggcagtcc ggggctggga atcttgtatc cccggagaat
    11101 acagaagcag aacagcagga gggctgccgc cagaaagcag gcgggaagga aaagggtgtg
    11161 ccattccccg cctgccgccg tcgttgtcag gaagatgtaa aaaagggcca gcaggcccgc
    11221 cagaattttc ccggcggcag tttcagcaat gcgcggagcg ggggaggtgc tggggggaga
    11281 gtctggcatg gttgggaatt tatctggaag gctgcagtat aaacgcggga aacttccttg
    11341 ccaagagcaa gctccctgtt gtatgcggga ggatgcgtcc gaatgccaga gttctggcgg
    11401 acaagacgga aaaggtgtcc gtaagccgga caggataaaa tgatccctgt tgccagggag
    11461 agtttctgcc ccttactgtg gctgaaataa aactgagtat cggtaaggga aaataatata
    11521 ccgcagaagc gggcatcaat ccgtctttgg ctgcatattt tccgttcgcc gtaaaatctt
    11581 gcagcgtttc agcctgtttt ttggaaaaca aggttccctt atgcctgtag gccgaattta
    11641 cgtttaattt ccggaacgga ataattcacc agcgtgggtt tgccccccgg ggtgcagtac
    11701 ggcacttcac aggcaagcaa atcctttagg atggcagggg cccggtgagg ggaaatgcgc
    11761 tccctccagg cggattttct ggcaaattcc ccaatgaagg tctcataagc cacgcgtttg
    11821 gcatttcggc tgaattcgga ctgggtgaga cggtccacca gctccaggag aaaagcgcgt
    11881 gcgttttcca gttccagcag tgcggggatg gattctattc tgatggtatt ctgtccaaag
    11941 ggcgtaacga ccatgccggc ctggtcaaaa tgcggggcga actgccggat gacggcaaaa
    12001 tcccgcgggt ccagatccag caccaccgga tccagaagct gctgggacgg catgggggct
    12061 tcccggcgtg cgcgcagccg ttcaaatatg atgcgttccc gggccgcctt gggatgcatc
    12121 agaaccaggc cttccggcgt ttcaaacagg gcgaattgct ggcggagtgt tcccagatag
    12181 gaaaatccgg cggaggcatc cctttctgaa gctgcgtttt catgggcgtt tcgtgccgtt
    12241 tcttcatcct cctgacggtg aaaatccagt ttcccctggg tggcgggaac ctgtttcaag
    12301 ggaatggccc ggagggtagg gggagggaca ggcccatggg attgtctggc agaggaggaa
    12361 agcggcggtt gggcggcgga agaagcaggg gccggtctgg cggccgccgg gaaagcagaa
    12421 ccggggttgg tggaacgggc gggtatttcc ccatacggcg cggtggtaga atgggcagga
    12481 ggaagagttc tctccggctc cggcgcggcg gcagcgtgga tttcctgccg ggcgtgcttt
    12541 tgaagagtgt tggctatggc ttccacaatg gtatttacca cgtctgcgga acggcggaat
    12601 ctcacttcct tcttggcggg gtgcacattg acgtccacca gagcgggctc cacctccata
    12661 tacaaaaaaa gcgccggatg cagtccggtg gggaagcctc catagccgtc ccgaatggcc
    12721 ctgttaatga gctggtcctc cacggggcgc gtgttcataa agacgtactg cccttttctg
    12781 gtgcgtctgg cttcggaaag aggaagcagg aatcccgtga tggaaatacc ggggccgatg
    12841 gtcgtttcta tcggaatcag ggccgccgct gtggcagcat ccgtcagtgc ggaaatgcgg
    12901 acgcgcagat ccgctgtggc gggaaggtca aaaacaagct ggtcgtcccg tttataggca
    12961 aaccgtactt ggggataagc cagggcatgc aggcgtatct ggtgctccac atgggaagct
    13021 tccgtttctg cggatttgag gaacttgcgg cgcgctggag tattgtaaaa caagtcggcc
    13081 acctcaatgg cggtgccggg agagacgcct gagcttctcg gttcgtgttc caggccgccg
    13141 tcaatgcgta tttcccagcc ttccagatcc tgctgctgcc tggtacagag tttgaagcgg
    13201 gaaacgctgg caatgctggg cagagcctct ccgcggaatc ccaggtgtgt gatttcaaaa
    13261 agctcctcca gggaagaaag tttgctggtg gcgtgccgtt tggtgcacag ctcggcgtct
    13321 gcccgtgaca tgccgctgcc gtcgtccgtc accttgatca tgcccacgcc tccgcggcgt
    13381 atctccaccc gtacgaattt ggctcctgca tccaggctgt tttccaccag ttccttcaca
    13441 acggaggcgg ggcgctccac tacttctcct gccgccactt ggctggcaag ggtgggagac
    13501 atgacgtgga tggatggcat ggcgatgacg gattggaaaa gactgttcca gaaagcggaa
    13561 ccctagccgc cggcgacgat ggagacaatt tccacccggt cgccgggaga aaccgtcacc
    13621 cgggaaaaat cctgcggcag gagcgcggcc ccgttgtgtt ccacgacgac gggtttgcct
    13681 ttcatgttca aaagttccag aagccgggag acggaacagg gttccgccag ttcgtacggg
    13741 gatccgttca gcataatatc gttctgggct gccatgacgg aagctactgt accacaacag
    13801 gagaaaaaat ccagaatcat cccgcgggga tggcataaaa aagattccgg tgcaagtcgg
    13861 aaaaaagaaa agctggcatc
//
LOCUS       DAOMSU010000028        13577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_196_length_13577_cov_3.394099, whole genome shotgun sequence.
ACCESSION   DAOMSU010000028 DAOMSU010000000
VERSION     DAOMSU010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13577
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_196_length_13577_cov_3.394099"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PK077_02355"
     CDS             <1..476
                     /locus_tag="PK077_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRN23172.1"
                     /translation="TNRALETADLVLEVADASMPRVKDHTAPALTAPRLLILNKCDLG
                     VHPDWKAVPGIRFSCATGEGRKELEEAIIQAFSSSLPGETGSSLVAINARHQHELGLC
                     REHVRLASESISRQESPEFTALELREALTHLGEITGAVDTEDVLGAIFSSFCLGK"
     gene            621..1478
                     /locus_tag="PK077_02360"
     CDS             621..1478
                     /locus_tag="PK077_02360"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF020596.2"
                     /note="This family includes IgM or IgG-cleaving cysteine
                     proteases; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IdeS/Mac family cysteine endopeptidase"
                     /protein_id="HRN23173.1"
                     /translation="MLRTLSLFFLFALVPVQAAIYFAHNVSEDSGFINTKKYWNGDSD
                     LCWAATASNMLQWWQNNSSGIPAFVPNGQNESGRTEIYDVFCNNWANTGKGIEIGLRW
                     YLGGKPLNPNNYLYDFKETITEPQNTGRYWEGYITSLGLSSSTWEGDCPFISSKYFTQ
                     SDFPLQFGTDLVSFFQNGGVVGLSIAPASGPGHAITCWGIEVDDTTGMAKSLYVTDSD
                     NGQGLEKRDVYYHETDGTLHLGSENGPRINAYDALMLPFYNVPEPSTAVLTTLAAGTA
                     FCRRRRRRS"
     gene            complement(1504..2133)
                     /locus_tag="PK077_02365"
     CDS             complement(1504..2133)
                     /locus_tag="PK077_02365"
                     /inference="COORDINATES: protein motif:HMM:NF015423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HRN23174.1"
                     /translation="MSTIIGALAGSIASSRVKMDLFGVIVCGTLAALGGGTVRDILLD
                     IPVYWTLPSGEIFVLASVVTSLATFYVAQKYPPPMGTIRVADAIVLALFGMIGTEKSY
                     LHGYTPTVSVMMGICTGVAGGLLRDVLTGNVPYVFRPGELYATAALLGGVAYAVLYYA
                     GIDSSTCFVTGFMVTLSVRLAAIRWNWNLPSYIPLFTSDTDSPPEEPET"
     gene            complement(2186..2965)
                     /locus_tag="PK077_02370"
     CDS             complement(2186..2965)
                     /locus_tag="PK077_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRN23175.1"
                     /translation="MMQFCVLGSGSGGNATIVRAGETVLLVDAGFSAARLRDKMKSAG
                     VEPDELDAILLTHEHADHMKGVHQFTRKHAVRVYATRHTAMCVQEKAPEAPWAYFEKG
                     QSFRIGDIVITPFPTYHDAVDPVGFKFETERSSLGFISDTGQAPGCIAEYLAMVDSLV
                     VESNYDPDMLAATPRRPWPLKQRIASAHGHLSNGQACDLLRRIAHGALKNVVLAHLSA
                     ESNSPALAESLMRDTLHDMGLASTSLFCASQDSCLPWIRVC"
     gene            3122..3577
                     /locus_tag="PK077_02375"
     CDS             3122..3577
                     /locus_tag="PK077_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23176.1"
                     /translation="MSSVSDNLFGAYTHKLDPKNRIAIPAEWRPSEGCALLLLSGRRL
                     DLPTVKAYTREKFQQLIDKIETTPGYTEAQIDLFIGKLYANCVEAVINAQGKLLIPKQ
                     MCEHAQLSSSVRLAARRGYFELWEPSLYEEVSRRENASISDINQSYGIL"
     gene            3587..5149
                     /gene="rsmH"
                     /locus_tag="PK077_02380"
     CDS             3587..5149
                     /gene="rsmH"
                     /locus_tag="PK077_02380"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRN23177.1"
                     /translation="MPPDSSILPNPQARPCATLALRPGWLASGLGVGARTGLHAELTA
                     YSIVFWGHDALPAQDMVAWQQEKARLMTEMSLAGQKGAYGKLGALSSGLNRAFDDHIE
                     LSLRAGAGTHVRTEEVQALANGLAEETSCRVLAWSVCRNHVHVIAELTEEKGVDELVC
                     SWKALAPSMNWEDAYHTEALKAREAAARVDALISELGDDAAAAVDAEADGADSGNGFR
                     HITVLLHETVDMLKAGPGKFIVDCTLGGGGHTELLLEQGATVWGIDRDADARRAAMLR
                     LARFGSRFKVLAGNFQDVESILSQQGVSRVDGLLADLGVSSHQLDTASRGFSFREDGP
                     LDMRMDTRAALSARNLVNEAPEEEIANILWQFGEERASRAIARTMVKARAQAPITTTA
                     QLARIVESVLPRKGRQHPGTRTFQALRIAVNGELDALDALLDSSVRLLGQGGRMAVIT
                     FHSLEDRRVKQFFDLRSRPEIDRPEWPAPRPNPDYCFRPLSRKPVTAGEEELSTNPRS
                     RSAKLRGVEKII"
     gene            5172..5516
                     /locus_tag="PK077_02385"
     CDS             5172..5516
                     /locus_tag="PK077_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23178.1"
                     /translation="MKRFKQRFTGHQISVSMIMWLIAFAALTCGAGITYAVLKNDQIQ
                     VARDIKQMNEEIASCELTKEYYRSKTSSLTSRWAIRDRLAQDKSNLQPIEPSAVEYIS
                     SENASRIAAAGH"
     gene            5533..7632
                     /locus_tag="PK077_02390"
     CDS             5533..7632
                     /locus_tag="PK077_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRN23179.1"
                     /translation="MITITKSRFARRSLVLCGLAGAAVIWLMLSLVNLQLVSPRKTSG
                     VSSGGIIEREVLLPQRGRIMDANEEILTSNMQSSELIADGYHLNDPKTISWALAYSKA
                     VHSPFWEKAATDKEKEKLVSGFRSKILGQAASKKDGSKEHNLAKILLEEPEDGPEGVD
                     MARKKLEELYEPEMVKEYVQAHLEYAAKVIAPFLPDMSVQDIINTVEKDGAIPKKRIV
                     IAKNLSEEKAELLRQAIQNARVQGFRFETSSKRVYSVPECMVHILGYIAQTKDSGPRP
                     VALSGLEKQLDDQLLGHNGIREYRKDSRGRIIPSADSRFKDAVDGLNVRLTVNMEYQT
                     IVEEELDAAISFYTDQTHKPRGCIIVVEPKTGSILAMASRPHYNLNTREGLQEGAYHF
                     AVQSLYEPGSTFKIVSVTSAVDSGKATFDTMINCTPYPVPGSRPVTDAPRSYGGLTVA
                     GVLKKSSNPGAFRIALKSGWPTYKKYFDLYGFSSKTGIDLPGETNSQCQDGSNFVNFS
                     RISFGYSLMVSPLQVAMAYAAIANDGVRMRPRLVDGIYVDDKNFQPSPPVEVCRVMSV
                     KTARDLRNALFHVTDLDGTAKRARIEGYNVGGKTGTAHKVKPTGGYFENRYTVSFVGM
                     LPVEDPAFVCLVVIDDPTSKHCHPGGGTVCAPVFQKLATRLAAAMNIPKNAPSAEANK
                     KASRAQASSTPSKPPRR"
     gene            7640..9145
                     /locus_tag="PK077_02395"
     CDS             7640..9145
                     /locus_tag="PK077_02395"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRN23180.1"
                     /translation="MKLLTLLKDLPSHTLTGSPQVKISHLECDSRRILPGSCYIALKG
                     TRADGHEFIPEAIRRGACAVVAEMPCTQEAREAGVSWVEVNDSRLAVSLMGAAWNGRP
                     SRHMTMIGVTGTNGKTTTAYIAHSLLKQSWLRAGLIGTIAYDNGEEITPSTHTTPGPL
                     ELEHLLKEMYENGCRGVSMEVSSHALDQERVAGINFNVGIFTNLTQDHLDYHHTMENY
                     FQAKAKLFEQMAQDTRSRRKPVAVINIDDAYGRRLAEMFSGRMTVKTYGSALGADFRM
                     LVHHATAKGSEYELEYKGKSYLVRVPLIGKFNMYNSLAALAAVICAGIPVRDAIANLQ
                     NIPQVPGRLELFTHPGGAQIFIDYAHTPDALENVCKTLKELCSRRLITVFGCGGDRDR
                     GKRPLMGAAAARLSDACIVTSDNPRSEEPLSIISQIAAGMPEGRYAIIPDRARAIATA
                     IDQARLGDIVLIAGKGHENYQELSTGRIDFSDAKEVRRNMFIKEREEFPQA"
     gene            9168..10541
                     /gene="murF"
                     /locus_tag="PK077_02400"
     CDS             9168..10541
                     /gene="murF"
                     /locus_tag="PK077_02400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRN23181.1"
                     /translation="MISLTAHGICNIIGGKLVSGPFDRVASGGVCTDSRHLPPHAVFF
                     ALGGEKFDGNLFAPEASRTAAAAVVSRVEEGMAPSCAVILVEDTLEALQRLAAWWRSE
                     LTLTVIGLTGSNGKTSTKDLTASVLSQGLRTIATQGNLNNHIGVPLSILRAAPSDEAA
                     VWEMGMNHSGELAPLCEMTRPKIGIITSIGTSHMEYLGSRENIAREKCTLAHCLPEDG
                     FMIFPADCDYADMIRQSTRAACIDCGIGAGTVRAENPVSTENGTRFILSIPDFCREEV
                     ELPVHGRHMVTNALLAAAAGWVAGLAKEQIASGLTQARLTGGRLHCSRINGILVVDDT
                     YNANPDSMQAALRTLAELSCAGRRFAVLGRMGELGVFSEEGHVLVGRTAEELRLDCVV
                     SVGTDAARITDAISPNSFTQRLNFSSAEEAAEWLRSHTAPGDIVLFKGSRLARMEQVM
                     NLTFPPQ"
     gene            10547..11650
                     /gene="mraY"
                     /locus_tag="PK077_02405"
     CDS             10547..11650
                     /gene="mraY"
                     /locus_tag="PK077_02405"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRN23182.1"
                     /translation="MHSLIEQFSPSGAFAGPAGRALLACLVSFALTMIFAPRVIRELI
                     SLKIGQPIRTAEEVHKLAELHGAKAGTPTMGGVLIVGSMTAATLLCARMGNPFIISCL
                     IVTLALGLLGFRDDYLKVAKKTSDGISARKKLLVQFLAGLAGVTFLYLYPEGSPRVEL
                     HDYISSLFIPFYGQVNLPWFVYIPFGVVVVMSASNAVNLTDGLDGLASGCSVATGITY
                     AIIAVLCGSWLTADSLDIPFHPGAGEISVFMMALVGACLGFLWHNCYPARVFMGDTGS
                     LALGGAFGMAAVCTAQELLFIVIGGVFVMEAVSVVLQVGSYKLRHGKRIFAMAPIHHH
                     FELKGWKETQVIARFWMISLLLAFLGLFLITTA"
     gene            11663..12973
                     /gene="murD"
                     /locus_tag="PK077_02410"
     CDS             11663..12973
                     /gene="murD"
                     /locus_tag="PK077_02410"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRN23183.1"
                     /translation="MQLNNLNIAVLGAGRSGRAAARLAMKHGARVCVFDASASIGGWP
                     EDIPLHTAATEEDGRAFHADMVVISPGIETDSPFVKSFGREGVETIGEMELACRFYHG
                     RIIAITGTNGKTTTTSLVEKILLRAGKTAVACGNYGVPMAEILLRDSVPDVLALEVSS
                     FQLETIRDFHPDVAVWLNFAPDHMDRYKSVEDYHRAKLHIFDNQTQQDLAVVRSGEQL
                     PQLKASVLTFSPENPAADLYYREPLIMEGGSPLLNLEGTALNQRHNAENAMAAVLACR
                     HLGIPAETAADVLKEFTPPGHRCETVRTLDGVLWLNDSKATNLHALEAALKSQHSPVI
                     LIAGGKDKGLDYVPLQPMLKEKVRACVVFGQIADQLQHAFSPAVPTEKAADVEACVAK
                     ARSLARPGDTVLFSPGTSSFDMFTGYVQRGQAFRDAVNTLSPLS"
     gene            12997..>13577
                     /locus_tag="PK077_02415"
     CDS             12997..>13577
                     /locus_tag="PK077_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23184.1"
                     /translation="MKTTKTPDHNPTGRTRPKRKMGTVLRAKLSKYRARVSEFEDDAP
                     SSTVVRWLVVLLLLHLLVIGGVYVRSTWFRNTTETVEMAAALPAPPTVPQRPAPAAPP
                     AALPQVPQAQPAAPVTITQPEQVVDARPNRQPAPVAEEHIPDAAPVAGPARHIVRTGD
                     TWERVARDNQVAVNDLKAVNPKVQRLVSGSTLV"
BASE COUNT         2919 a         4299 c         3757 g         2602 t
ORIGIN      
        1 gcaccaacag ggcgcttgaa accgcagatc tggttctgga agtggcggat gcctccatgc
       61 cccgggtaaa agaccacacc gcccctgccc tcaccgcgcc ccggctgctt attctgaaca
      121 agtgcgacct gggagtccat ccggactgga aggccgtgcc cggcatccgg ttttcctgcg
      181 ccaccgggga aggaagaaag gaattggagg aagccattat ccaggccttt tcctcctccc
      241 tgcccggtga aacgggcagc tccctggtag ccatcaatgc ccggcaccag catgaactgg
      301 gcctctgccg ggagcatgtc cggctggctt cggaatccat ctcccgccag gaaagtccgg
      361 aattcacagc cctggaactg agagaggccc tgacgcacct gggagaaatc accggagccg
      421 tggatacgga ggacgtgctg ggcgccattt tttcctcctt ctgcctgggg aaataagaag
      481 aagctttcat caacaggttt ttctgactcc agattcttct ttcctctcca ttctgtggaa
      541 ggatggcaat catccaaaaa tttatttgcg gatattttca ttgattccgg cttgttaatt
      601 cgctatcgat atcatgtttt atgttgcgca ccctttctct ctttttcctt ttcgctctgg
      661 ttcctgttca agccgccatt tattttgccc ataatgtttc cgaagattcg gggttcataa
      721 ataccaaaaa atactggaac ggagattccg atttatgctg ggcggccaca gccagcaata
      781 tgctgcaatg gtggcagaat aattcctccg gcattccggc tttcgttccc aacggccaga
      841 atgaaagcgg aagaacggaa atttacgatg tgttttgcaa taattgggcc aataccggga
      901 aaggcataga aataggcctg cggtggtacc tgggagggaa acctctcaat cccaacaatt
      961 atttatacga ttttaaggaa accatcactg aaccgcagaa tactggccga tactgggagg
     1021 gatacattac atctctggga ttgtcctctt ccacctggga aggcgactgc cctttcatca
     1081 gcagcaaata cttcacgcaa agcgactttc ccctccagtt cggtacagac ctcgtctcat
     1141 ttttccagaa tggcggcgta gtgggacttt ccattgcccc tgcatcaggt ccgggtcatg
     1201 ccattacctg ctggggaatt gaggttgacg acactaccgg catggccaaa tccctgtacg
     1261 tcacagactc cgacaacgga caaggtctgg aaaaacggga cgtttattat catgaaacgg
     1321 atggaaccct gcacttgggg agcgagaacg gccctcgaat caacgcatac gacgccctga
     1381 tgctgccctt ttataatgtt ccggaacctt ccacggcagt tctcaccacc ctcgcggcgg
     1441 gaactgcctt ttgccgccgc aggcgccgac gctcttaagg cttcacgaaa acattccctc
     1501 ctgtcaagtt tccggttctt caggaggcga atcggtatcc gaggtgaata acggaatgta
     1561 ggaaggcagg ttccagttcc agcggatggc cgccaggcgt acggaaagcg tgaccatgaa
     1621 gcccgtcacg aagcaggtgg aagaatcaat tccggcatag taaagcaccg cgtacgccac
     1681 gcctcccagc agggccgcgg tggcgtatag ttctccggga cggaacacgt agggaacgtt
     1741 gccggtaagc acgtcgcgca gcaggccgcc cgccacgccg gtacaaatgc ccatcatcac
     1801 ggaaacagtg ggcgtatagc cgtggagata ggatttttcc gtgccgatca tgccgaaaag
     1861 ggccagaacg atagcatccg ccacgcggat agtgcccatg ggtggcggat atttctgggc
     1921 cacataaaag gttgccagac tggtgacgac ggaggccaga acaaaaattt cccctgaggg
     1981 gagcgtccag tacaccggta tgtccagcag gatgtcacgc acggttccgc cgcccagagc
     2041 agccaaggtg ccgcagacaa tcacgccgaa caaatccatt ttcacccgcg aggaagcgat
     2101 ggaacccgcc agggctccaa taatcgtgct catgatttca caggtggaga ggaacatggc
     2161 gggaggaaca ggaaaagagg catattcagc agacccgtat ccacggcagg caggagtcct
     2221 ggctggcgca gaacagggag gtggaagcca gccccatgtc atgcagggta tcacgcatca
     2281 ggctttctgc caatgccgga gaattgcttt ctgcgctcag gtgcgccagc accacgtttt
     2341 tcagggctcc atgagcaatc cgcctcagca ggtcgcacgc ctgcccattg gagagatgac
     2401 cgtgcgcaga ggcgatgcgc tgcttcagcg gccaaggtct cctgggagtg gccgccagca
     2461 tgtccggatc atagtttgat tccaccacca ggctgtccac catggcaagg tattccgcta
     2521 tgcagccggg agcctgcccc gtatcggaaa tgaaacccag gctggaccgc tccgtttcaa
     2581 atttgaatcc caccgggtcc acggcgtcat ggtaagtggg gaagggagta atgacgatat
     2641 cccctattct gaaagactgc cccttttcaa aataggccca gggagcctcc ggcgcttttt
     2701 cctggacgca catggccgta tggcgtgtgg cgtaaacacg cacggcatgc tttctggtga
     2761 actggtgcac gcctttcatg tggtccgcgt gttcgtgggt aagcagaatg gcgtccagct
     2821 catccggctc cactccggcg gacttcatct tatcccgcag cctggcggcg ctgaacccgg
     2881 catccaccag caggacggtt tcccccgccc tgacaatggt ggcatttccc ccgctgccgc
     2941 tgcccaacac gcaaaactgc atcatgcgcg gaatataata ccgcccccgg aagggagcaa
     3001 gaacgtatcc atgctttttt tatgacatgg gcacatgaaa aaatactttt ttccagtacg
     3061 gggttgacat ttcaggtgca ttaaagtgta ttgaagtcac tacgtagtgt acttgcatgc
     3121 gatgagcagt gtatcagaca acctttttgg tgcatacacg cacaagctgg accccaaaaa
     3181 ccggattgcc attccggccg aatggaggcc ctccgaaggt tgcgccctgc tcctgttatc
     3241 cggccggcgt cttgatttgc cgaccgtcaa agcctatacg cgggaaaaat tccagcaact
     3301 cattgacaag atagaaacaa cgcccggcta cacggaagcg caaatcgacc ttttcatcgg
     3361 caaattatac gccaactgtg tggaggctgt catcaacgcc caaggcaagc tgctgatacc
     3421 caaacaaatg tgcgaacacg cacagctcag ttcctccgtc cggctcgccg cacggagagg
     3481 atatttcgaa ttgtgggaac cctctctcta cgaagaagtt tcccggcgtg aaaacgccag
     3541 catctccgac atcaaccaat cctacggcat tctctagctt tccttcatgc ccccagactc
     3601 ttccattctg cccaatcccc aggcacgccc ctgcgcaacg cttgcattgc gcccgggctg
     3661 gcttgcttcc ggcctgggcg tcggcgccag aaccgggctg catgcggaac tcacggctta
     3721 ttccatcgtt ttctgggggc acgatgccct tccggcgcag gatatggttg cctggcagca
     3781 ggaaaaggca cgcctcatga cggagatgtc cctggccggg caaaagggcg cctatggcaa
     3841 gctgggcgca ttgtcttccg gcctgaaccg ggcttttgac gaccatattg agctttcttt
     3901 acgcgcgggc gcgggtacgc acgtccgcac ggaggaggtg caggccctgg caaacgggct
     3961 cgcggaagaa acatcctgcc gcgtgctggc gtggagcgtc tgccgcaacc atgtgcatgt
     4021 gattgcggag ctgacggagg aaaaaggagt ggatgaactg gtctgctcct ggaaggcgct
     4081 ggccccctcc atgaactggg aagacgcgta ccacacggaa gccctgaaag cccgggaagc
     4141 cgcggccagg gtagacgccc tgatttccga actgggggat gacgccgctg ccgccgttga
     4201 tgcggaagcg gacggggcag actccggaaa cggcttccgg cacattaccg tgctgctgca
     4261 tgagacggtg gacatgctga aagcgggccc cggcaaattc atcgtggact gcacgcttgg
     4321 cggcggcggc catacggaac ttctgctgga acagggcgcc accgtctggg gcattgaccg
     4381 cgatgcggac gcccgccgcg ccgccatgct caggctggcg cgcttcggtt cccgtttcaa
     4441 agtcctcgcc ggcaacttcc aagacgtgga aagcattctt tcccagcagg gggtttcccg
     4501 ggtggacggg ctgctggcgg acctgggcgt ttcctcccac cagcttgaca ccgcctcccg
     4561 cggcttttcc tttagggaag acggccctct ggacatgcgg atggataccc gcgccgctct
     4621 ctctgcccgg aacctggtga atgaggcccc ggaagaagaa atcgcaaata tcctctggca
     4681 attcggcgag gaacgcgctt cccgcgccat tgcccgcacc atggtgaaag cccgtgccca
     4741 ggcacccatc accacgaccg cacagcttgc ccgcatcgtg gaatccgttc tgccgcgcaa
     4801 aggaaggcag caccccggca cccgcacgtt ccaggccctg agaatagccg tcaacggcga
     4861 gctggatgcc ctggacgctc tgctggattc ctccgtgcgt ctgctcgggc agggggggcg
     4921 catggccgtc atcaccttcc acagcctgga agaccgcagg gtcaagcagt tcttcgacct
     4981 ccggagccgt ccggaaatag accgcccgga atggccggca ccccgtccca acccggatta
     5041 ctgcttccgc ccgctttccc gcaaacccgt cactgcggga gaagaagaac tttcaaccaa
     5101 cccccgttcc cgcagcgcca agttgcgcgg cgtggaaaaa atcatctgac catctctctt
     5161 ccgccaccgc catgaaaaga ttcaaacagc gtttcaccgg ccaccagatc agcgtgagca
     5221 tgatcatgtg gctcattgcc ttcgccgccc tcacctgcgg agcgggcatc acttatgccg
     5281 tgctgaaaaa cgaccagatc caggtcgcgc gcgacatcaa gcaaatgaat gaagaaatcg
     5341 cctcctgcga actcacaaag gaatattacc gttccaagac ttcctccctg accagccgct
     5401 gggccatccg cgacaggctg gcccaggaca agtccaacct gcagcctatc gagccctcag
     5461 ccgtagaata catttcttcc gaaaacgcct cccgcatcgc tgcggcaggc cattaaacgc
     5521 gttgagccgt tcatgatcac gatcacgaag tccagatttg ccagaagaag cctcgtcctg
     5581 tgcggcctgg cgggcgcggc ggtcatctgg ctgatgttgt cgctggtcaa tcttcagctg
     5641 gtttcccccc ggaaaacgag cggcgtttcc agcggcggca ttattgaacg ggaggttctt
     5701 ttgccccagc gcggccgcat catggacgcc aatgaggaaa tcctcaccag caacatgcag
     5761 agttccgagc tgattgcgga cggctatcat ctgaacgacc ccaagaccat cagctgggct
     5821 ctggcctatt ccaaggccgt tcattccccg ttctgggaga aagccgcaac ggataaggaa
     5881 aaggaaaagc tcgtttccgg cttccgcagc aagattctgg gccaggccgc cagcaaaaag
     5941 gacggcagca aggaacataa tctggcgaaa atcctgctgg aagagcctga agacggaccg
     6001 gaaggggtgg atatggccag gaaaaagctg gaagagcttt atgagccgga gatggtgaaa
     6061 gaatacgtgc aggcgcacct ggaatacgcc gccaaagtga ttgccccttt cctgccggac
     6121 atgagcgtgc aggacatcat caacacggtg gaaaaagacg gggcgattcc caaaaagcgc
     6181 atcgtcattg ccaaaaacct gtccgaggaa aaagcggaac tgctgcggca ggctatccaa
     6241 aacgcccgag tccaggggtt ccgttttgag acctcctcca aacgcgtcta ttccgtgccg
     6301 gaatgcatgg tgcatattct gggctacatc gcccagacga aggacagcgg cccccggccc
     6361 gtagcccttt ccggccttga aaagcagctg gacgaccagc tgctgggcca caacggcatc
     6421 agggaatacc ggaaagacag ccgcgggcgc atcattccct ccgcggattc ccgcttcaag
     6481 gatgccgtgg acgggctgaa cgttcgcctg acggtaaaca tggaatacca gacgatcgtg
     6541 gaggaggagc tggacgccgc catctctttt tacacggacc agacccacaa accccgcggc
     6601 tgcattattg tggtggaacc caagaccggc agcattctgg cgatggccag ccggccccac
     6661 tataatttaa atacgcgcga gggccttcag gaaggcgcct accatttcgc cgtgcagtcc
     6721 ctgtacgaac ccggctccac ctttaaaata gtttccgtca catccgccgt ggacagcgga
     6781 aaggccacgt tcgacaccat gatcaactgc accccctatc cggtgcccgg ttcccgcccc
     6841 gtcacggatg ccccccgttc ctatggcggt ctgaccgtag cgggcgtgct gaaaaaatcc
     6901 agcaacccgg gagccttccg cattgcgctg aaatcgggct ggcccacgta taaaaaatac
     6961 tttgacctgt acggattttc ctccaaaacg ggtatcgacc tgccggggga aaccaacagc
     7021 cagtgccagg acggcagcaa ttttgtcaac ttttcccgca tcagcttcgg ctactccctg
     7081 atggtgtccc ccctgcaggt cgccatggct tatgccgcca tagccaatga cggcgtgcgc
     7141 atgcgccccc ggctggttga cggcatttat gtggatgaca agaacttcca gccttccccc
     7201 cccgtggagg tatgccgggt catgagcgtc aagacggccc gggaccttcg gaacgccctg
     7261 ttccacgtta cggatctgga cgggaccgcc aaaagagctc ggattgaagg ctataacgtg
     7321 ggaggcaaaa ccggaaccgc ccacaaggtg aagcctacag gcggctattt tgaaaaccgc
     7381 tacaccgtct cctttgtagg aatgctcccg gtggaggacc cggccttcgt ctgccttgtc
     7441 gtcattgacg accccacttc caagcattgc catcccggag gcggaaccgt atgcgccccg
     7501 gttttccaga agctggccac ccgtctggcg gccgccatga acatacccaa gaatgcccct
     7561 tccgcggagg caaataaaaa agcctcccgt gcccaggcct cttccacccc atccaaaccg
     7621 ccgcgcagat aatcccgtca tgaagctact taccttactc aaagaccttc cctcccatac
     7681 gctcaccggc tccccccagg tgaaaatttc acatctggag tgcgacagcc ggcgcattct
     7741 gcccggttcc tgctacattg ccctgaaggg gacccgggcg gatggccatg aattcatccc
     7801 ggaggccatt cgccgcggag catgcgccgt cgtcgcggaa atgccctgca cgcaggaagc
     7861 cagggaggcc ggagtcagct gggtggaggt gaacgattcc cgcctggccg tcagcctgat
     7921 gggcgcggcc tggaacggac gcccttcccg ccacatgacc atgatcggcg tgacggggac
     7981 gaacggaaaa actacgacgg cctacatcgc ccattccctg ctcaagcagt cctggctgcg
     8041 cgccggattg atcggcacca tcgcctatga caacggagag gaaatcactc cttccaccca
     8101 taccacgccc ggaccgctgg aactggaaca cctgctgaag gaaatgtatg aaaacggctg
     8161 ccgcggcgtc tccatggagg tctcttccca cgcgctggac caggaacgcg tggccggcat
     8221 caatttcaac gtgggcattt tcaccaatct gacccaggac cacttggact accaccacac
     8281 tatggagaat tatttccaag ccaaggcgaa attgttcgaa cagatggcgc aggacaccag
     8341 atcccggcga aaacccgtgg ccgtcatcaa catcgacgac gcttacggcc gccggctggc
     8401 ggaaatgttc tccggccgca tgaccgtaaa gacctacggg tccgccctgg gggcggattt
     8461 ccgcatgctg gtgcaccacg ccaccgccaa aggcagcgag tacgaattgg aatacaaggg
     8521 gaaaagctac ctggtccgtg tccccctgat cggcaagttc aacatgtaca acagcctggc
     8581 cgcgctggcc gccgtcattt gcgccggcat ccccgtgcgg gacgccattg cgaaccttca
     8641 gaatattccg caggtccccg gcaggctgga actattcacc caccccggag gcgcccaaat
     8701 cttcattgac tatgcccaca cgccggacgc cctggaaaac gtctgcaaga cgctcaagga
     8761 actgtgctcc cgccgcctga ttaccgtgtt cggctgcggc ggggaccggg acaggggaaa
     8821 acgtcccctg atgggagccg cagccgcacg cctgtcagac gcctgcatcg tcacctccga
     8881 caatcccaga agcgaggaac ccctctccat catcagccag attgcggcgg gaatgccgga
     8941 aggcagatac gccatcattc cggaccgggc cagagccatt gccacggcca tcgaccaggc
     9001 ccgtctgggg gacatcgtcc tcattgcggg caagggccat gaaaattacc aggaactttc
     9061 caccggccgc attgatttca gcgacgccaa ggaagtgcgc cgcaacatgt tcatcaagga
     9121 acgggaagaa ttcccccagg cataacacgt tattccatct ttctttcatg atttccctca
     9181 cggcgcacgg catctgcaac atcatcggcg gaaaactggt cagtggcccc tttgacaggg
     9241 tcgcttccgg cggcgtgtgc acggacagcc gccatttgcc gccccatgcc gttttcttcg
     9301 cgctgggggg agaaaaattt gacggcaacc tgtttgcccc ggaggcatcc cgtaccgcgg
     9361 ccgccgccgt cgtcagccgt gtggaggaag gcatggcccc ctcatgcgcc gtcattctgg
     9421 tggaagacac gctggaagcc cttcagaggc tggccgcctg gtggcgttca gagcttaccc
     9481 tcaccgtcat cggcctgacc ggctccaacg gaaaaacctc taccaaggat ctgacggcct
     9541 ccgttctttc acaggggctc cgcaccatcg ccacgcaggg caacctgaac aaccacatcg
     9601 gcgtccccct cagcatcctc cgggccgccc cgtccgacga agccgccgta tgggaaatgg
     9661 gcatgaacca ctccggggaa ctggctcccc tgtgtgaaat gacgcgcccg aaaatcggca
     9721 tcatcaccag catcggcacc tcccatatgg aatacctggg ctcccgggaa aacatcgccc
     9781 gggaaaaatg cacgttggca cactgcctgc cggaagacgg cttcatgatt tttccggcgg
     9841 actgcgatta tgcggatatg atccgtcaat ccacccgcgc cgcctgtatt gactgcggca
     9901 ttggcgccgg aaccgttcgc gcggaaaatc ccgtctctac cgagaacgga acacgcttca
     9961 tcctttccat cccggatttc tgccgggaag aggtggaatt gcccgtccac ggccggcaca
    10021 tggtaaccaa cgcgcttctg gccgccgccg ccggctgggt agcaggtctt gcgaaagagc
    10081 aaatcgcctc cggtctcacc caggcgcggc ttacgggcgg aaggcttcac tgctcccgga
    10141 tcaacggcat tctggtagtg gatgacacct acaacgccaa cccggattcc atgcaggccg
    10201 ccctgcgcac cttagcagag ctttcctgcg ccggcagacg ctttgcggtg ctgggcagaa
    10261 tgggggaact gggcgtgttt tccgaggaag ggcatgttct ggtgggccgc acggcggaag
    10321 aactccgtct tgactgcgtc gtcagcgtgg gaacggacgc cgcccggatt acggacgcta
    10381 tttctcccaa ttcattcact caacgcctga acttcagctc cgcggaagaa gcggccgaat
    10441 ggctccgcag ccatacggcg ccgggagaca tcgtcctctt caaaggcagc cgtcttgccc
    10501 ggatggagca agtcatgaac cttacctttc cgccacagta aattgtatgc attccctcat
    10561 tgaacagttc agcccctcag gggcatttgc aggaccggca gggagagccc tcctggcctg
    10621 cctggtttcc tttgccctga cgatgatctt cgccccgcgg gtcatccggg agctgatttc
    10681 cctgaaaatc ggccagccca tcaggacggc ggaggaagtg cacaagctgg cggaactcca
    10741 cggagccaag gcgggaacgc ccaccatggg cggcgtgctg atcgtgggtt ccatgacggc
    10801 ggctaccctg ttgtgcgcca gaatgggcaa tccctttatc atttcctgcc tgatcgtcac
    10861 cctggcgctg ggcctgctgg gattccggga cgattacctg aaggtggcga aaaaaacctc
    10921 ggacggcatt tccgcacgta aaaaactcct cgtccagttc ctggccggcc tggccggcgt
    10981 caccttcctg tacctgtacc cggaaggcag tccccgcgtg gaacttcacg actacatatc
    11041 ctccctgttc attccattct atggccaggt gaacctgcca tggttcgttt acattccttt
    11101 cggcgtcgtg gtggtcatgt ccgcctccaa tgcggtgaac ctgacggacg gcctggacgg
    11161 ccttgcctcc ggctgctctg tcgccacggg catcacttac gccatcattg ccgtcctctg
    11221 cggaagctgg ctgacggctg attccctgga tattcccttc caccccggcg cgggagaaat
    11281 cagcgtcttc atgatggccc tggttggggc ctgcctgggc ttcctgtggc acaactgcta
    11341 cccggccagg gtatttatgg gagacaccgg ttccctggcc ctgggcggcg cgttcggcat
    11401 ggctgccgtc tgcacggcgc aggagcttct ctttatcgtc atcgggggcg tcttcgtcat
    11461 ggaagccgtg tccgtcgtcc tgcaggtggg cagctacaag ctgcgccacg gcaaacgcat
    11521 cttcgccatg gcccccatcc accaccactt cgaactcaaa ggctggaagg aaacgcaggt
    11581 gattgcccgc ttctggatga tcagcctcct gctggctttc ctgggccttt tcctgataac
    11641 caccgcctga caaaccacga acatgcagct caataacctt aacatcgccg ttctgggagc
    11701 gggaagaagc ggacgcgccg cggcgcgcct cgccatgaag catggcgcac gggtatgcgt
    11761 gtttgacgct tccgcctcca ttggcggatg gccggaagac attcccctgc ataccgccgc
    11821 cacggaggaa gacggacgcg cctttcatgc ggatatggtg gtcatctccc ccggcattga
    11881 gacggatagc ccctttgtca aatcctttgg acgggaaggc gtggaaacca tcggtgaaat
    11941 ggagctggca tgccgctttt accatggccg catcatcgcc atcaccggaa ccaacggcaa
    12001 aaccaccacc acatccctgg tggaaaagat tttgctgcgc gccggaaaga ccgccgttgc
    12061 ctgcggcaat tacggcgtgc cgatggcgga aatcctgctc cgggattccg tgccggacgt
    12121 gctggcgctg gaggtcagct ccttccagtt ggaaaccatc cgcgattttc acccggatgt
    12181 ggccgtatgg ctcaattttg cgccggacca catggaccgc tacaaatccg tggaggatta
    12241 ccaccgcgcc aaactacata tcttcgacaa ccagacgcaa caagacctgg ccgtcgttcg
    12301 ctccggagaa cagcttccac agttgaaagc ctccgtgctc accttcagtc cggaaaatcc
    12361 ggcggctgac ctttattacc gcgaaccgtt gatcatggag ggcggctccc ccctgctgaa
    12421 cctggaaggg accgccctga accagcgcca taatgcggaa aacgccatgg ccgccgtcct
    12481 ggcatgccgc cacctgggca ttcccgcaga aacagccgca gatgtattga aggagttcac
    12541 gccccccggc caccgctgcg aaaccgtccg tacactggac ggcgtgctgt ggctgaacga
    12601 ttccaaagcc acgaatctgc atgcgctgga agcggctctg aaatcacagc attcccccgt
    12661 catcctgatt gccggaggca aggacaaagg actggattac gtgcccctgc agcccatgct
    12721 gaaagaaaaa gtgcgcgcct gcgtggtatt cggccagatt gcggaccagc tccagcacgc
    12781 tttttccccc gcagtcccca cggaaaaagc cgcagatgta gaggcctgcg tggcaaaggc
    12841 ccgctctctc gcccggccgg gagacaccgt cctattctcc cccggcactt cttcttttga
    12901 catgttcacc gggtatgtcc agcgcggcca ggccttccgg gacgcggtga acaccctctc
    12961 ccccctttcc tgacaattca ccagaaaacg accatcatga aaacgaccaa gacacctgat
    13021 cacaatccga ccggacgcac ccgtccgaaa cgcaaaatgg gcaccgtact ccgcgccaaa
    13081 ctcagcaaat accgcgcccg cgtctccgaa tttgaagacg acgcgcccag cagcactgtc
    13141 gtgcgctggc tcgtcgtcct tctccttctt caccttctcg tcatcggcgg cgtttacgtc
    13201 cgcagcacct ggttcagaaa cacgacggaa accgtagaaa tggcggccgc cctgcccgcg
    13261 ccgcccaccg tcccgcagcg ccccgctccc gctgcgcccc cggcagccct gccccaggtt
    13321 ccccaggccc aacccgcggc cccggtcacc atcacgcagc cggaacaggt agtggacgcg
    13381 cgccccaaca ggcagccggc ccccgtggcg gaggaacaca ttccggatgc ggctccggtg
    13441 gcaggtcctg cgcgccacat cgtgcgcacg ggggatacct gggaacgcgt cgcgcgtgac
    13501 aaccaggtgg ccgtcaatga cctgaaagcc gtcaatccca aagttcagcg ccttgtcagc
    13561 ggaagcacgc tcgtcat
//
LOCUS       DAOMSU010000029        13430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_203_length_13430_cov_2.474991, whole genome shotgun sequence.
ACCESSION   DAOMSU010000029 DAOMSU010000000
VERSION     DAOMSU010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13430
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_203_length_13430_cov_2.474991"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PK077_02420"
     CDS             complement(<1..623)
                     /locus_tag="PK077_02420"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRN23185.1"
                     /translation="MNIGVLNSGGDSPGLNAVIEGVVGAASRRGWNVVGFYDGFEGLL
                     SEEGDERFEWLTPAACRGLRAKGGTILGTVNKGNFAIKVGVDQKGAIEPAVLEKTKAT
                     VRRLGLDALIVVGGDGSQSTALLLSEIGLPVVGVPKTIDNDLGATDVTFGFYSAVSIV
                     SESLDRLETTANAHQRMMVVEVMGRHAGWIALEGGIAGSADVILLPE"
     gene            complement(668..1639)
                     /locus_tag="PK077_02425"
     CDS             complement(668..1639)
                     /locus_tag="PK077_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRN23186.1"
                     /translation="MKKLSLFLLTLACVFQFSAQAQERPSYIAIEANSGKLLFSSNAE
                     EKRPVASLSQVATAMVALDWVARTRLPLDTFIAVPQEAFGLRAGNPMDLQPGDRLTLR
                     DALYSTLLGSDNVSALTVASFVGRDLVSRRGGGAPIAAFVAEMNNLARSLRMAKTRFT
                     APHGLDAGNSVSTSCALDMALLGVYSMQNPAFCYIVSQASRRIEVQSQTKGRTMYDIS
                     NNNRLMSASGVDGIKAGTSRAAGPCLLLSVTRNAISRRSPQTGTEVIYPQRMIIAVLG
                     SASRYSLAREMMNTGWRVWENWQASGMDMKDPKEFVQLPVKSAPQRR"
     gene            1813..2223
                     /locus_tag="PK077_02430"
     CDS             1813..2223
                     /locus_tag="PK077_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23187.1"
                     /translation="MTRIQLFYSRAYPGNTLRANTLQALAHLGVNPEMQVEETVPEQI
                     GTPLPALAIDGEIVVYGVEPSVHELELLLQDRVQEREADFPCGACGMGCSGMNSPENC
                     PMCIQDGKDGSMAGRIIGFAILLMLLFFAMKILS"
     gene            2384..2644
                     /locus_tag="PK077_02435"
     CDS             2384..2644
                     /locus_tag="PK077_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23188.1"
                     /translation="MRSFFGSILLILVLAALGATIYYHWSTNSHLEFELRNPDEPEKT
                     IQVINMEKRAAALQAVPGEKTPVSPAEEIPASAAGMPEPAEL"
     gene            2664..3533
                     /gene="pdxA"
                     /locus_tag="PK077_02440"
     CDS             2664..3533
                     /gene="pdxA"
                     /locus_tag="PK077_02440"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HRN23189.1"
                     /translation="MKPVIGYTLGDQSGIGPEVISAALASGELPEDAEYRLIGKRVEL
                     PPGRPNAESAKHAFDHLEQAAHALREGTVDAVVTAPVCKETLHEAGFRWPGQTEFFAE
                     RLETDNYAMCLTGKRLTVGLATIHTSLQSVPSLLETEELVRIGTLLKDFCIRKGIMRP
                     RIALAALNPHAGEHGAFGDEDGNIIAPAVGRLRAIHPDAAFDGPSVPDCVYRDAVEGN
                     YDAVLAPYHDQGLIPLKLVDFHTAVNVTLGLPRPRLSPDHGTAFNLAGTGKANPSSTI
                     HAFQLAVQMAQTR"
     gene            3969..4253
                     /gene="rpsF"
                     /locus_tag="PK077_02445"
     CDS             3969..4253
                     /gene="rpsF"
                     /locus_tag="PK077_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRN23190.1"
                     /translation="MRNYEALIVFNMKGTETPVEELISTVAAAMKEEGADITATENAG
                     RREFAYESNHLSAGQYVTYTFSAEPSAIRPIRERLRLNPQIHLQYYKVLG"
     gene            complement(4649..>5235)
                     /gene="hisH"
                     /locus_tag="PK077_02450"
     CDS             complement(4649..>5235)
                     /gene="hisH"
                     /locus_tag="PK077_02450"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HRN23191.1"
                     /translation="GAGNLRSVLNTFEAAGVTGHLVRTPEDAAGVTHLVLPGVGAFGD
                     CAEKLRRQNLVPLVRAWIEQDRPFLGICVGYQVLFESGEESGQVPGLGILKGRVVRFS
                     ESELKVPHMGWNSVALTDPSDPIWKGMGKEPYFYFVHSYYPQPEDKSLIAAFCTYGVR
                     FAAAVRRGNLVATQFHPEKSQKLGVKLLKNFISL"
     assembly_gap    5236..5286
                     /estimated_length=51
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5287..5818)
                     /gene="hisB"
                     /locus_tag="PK077_02455"
     CDS             complement(<5287..5818)
                     /gene="hisB"
                     /locus_tag="PK077_02455"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HRN23192.1"
                     /translation="MRNASCKRVTGETDISMELNLDGTGCAAVATGHAFFDHMLDLLA
                     RHSLMDLTLQARGDLEVDAHHTVEDVGIVLGECIKNALGDKKGIVRYGCSYLPMDETL
                     TRVVMDLSNRPYVAFRIPEGGLPDAPNFPLTLCEEFCRALANNLRCNLHVEVLYGRDG
                     HHIAESVFKGIAHALRQ"
     gene            complement(6101..7327)
                     /locus_tag="PK077_02460"
     CDS             complement(6101..7327)
                     /locus_tag="PK077_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23193.1"
                     /translation="MNKSLILVLTAFVPLAAADEVKLKDGTVYKNCTVEVETPESVNL
                     LVPVSGGIKDSVTVKRDLIESIRKATPDELEAARIGKMYAKPETMNAGDLEKALADLD
                     KTIKKNPQGLAHDAAVKARAKVVVLLEEKKLTEEAQAAQNAREEAEVTVRTKYDHEAN
                     KLLKRFKALAVRNPYQAMAVYDRLRDGYPGSAALADAYPDAARIAGQLNRKLEVMIAA
                     KEKSLEKEREALRKEEEKRRGNPKLTKEQRQVLMDAFQKRQTAIRERENQLTEVYRAL
                     RKKVKERGDRWFEPTAGSLEAMRDLKLVAATDAERLKKQDPENGAGSAALEKAWNLCD
                     EKKFDDAMEVLTELRTAQVPREYYEELTETVRVGLREQRARERAERAEAARKAREDRD
                     KKRQEEREAAAKAKKK"
     gene            complement(7469..8074)
                     /locus_tag="PK077_02465"
     CDS             complement(7469..8074)
                     /locus_tag="PK077_02465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRN23194.1"
                     /translation="MATSHSLKAETRACGSGNLKQLRSQGLVPGVVYGPGFDNVNIQV
                     DAREFARMLASAVSEHILVALDINGKIVKVLLKEVQHNPITNACLHVDFQAVTDTTVI
                     HSIVPVILEGDSAGVALGGVLDQTIHELAIICQVKDLPEAITADISGLKLGESLRITD
                     LKLPSGVTTELAGDVIVAIVEAPRVSGEEAAPAAEEAVAEK"
     gene            complement(8208..9137)
                     /locus_tag="PK077_02470"
     CDS             complement(8208..9137)
                     /locus_tag="PK077_02470"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HRN23195.1"
                     /translation="MKIISGTAHRELAERIAQSVGIQLTNVTVNTFPDGESFVKINEN
                     IRGKDVFLIQPTCPPTNHNIMELCVMVDAARRASAGRITAVVPFFGYARQDRKDQPRV
                     PITAKLVANLLTAAGVDRVLTMDLHAAQIQGFFDIPVDHLYAAPVLIRHLREHYVKDL
                     KKLVVVSPDVGGVKMARAYSDALGAELAIVAKHRVNATHVEAMNVIGDVEGRDAVLID
                     DMTETAGTLCAAANILKERGAQRVFACVSHGVLGDMARERISGSCIERVLTSDTVPMA
                     YGPKVDCVSVGDLLGHAVQRIHDGESVSSLFDI"
     gene            9220..10170
                     /locus_tag="PK077_02475"
     CDS             9220..10170
                     /locus_tag="PK077_02475"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HRN23196.1"
                     /translation="MMDSPAPDGKERFLTAPMIAALAVSTLLLALNYLAFPEFGSFGN
                     ENSIQWLISSWNKQTDYEHGWLVVPIIVFMLYHARKKIAQAPRRMDWRGLILFIPAIM
                     LLMLSFRVGQPRVAVGALPLILLGGAWYLAGPQTGRLCAFPLLFFWLCIPLPSFQQAT
                     VGLQIIATELGHWGATIFGVDTYLQGTNIRSTGGHWDAFNIAGGCSGMRSLMALLMLS
                     AAWAYLSDLKFWKKCVLFLSAIPLAVIGNGVRITSIVVMAEYGNPEFASKTWHDWSGL
                     LFFFPISLFGLAAVHSLLAGELIWKPSQRKKLVVKMNKSH"
     gene            10185..10907
                     /locus_tag="PK077_02480"
     CDS             10185..10907
                     /locus_tag="PK077_02480"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HRN23197.1"
                     /translation="MNIKTLKILIIPFLLAVMLGGTYLMPRRGEVQDSSISMDLPTGM
                     NPSGWHGTRRQESEQERGILAPDTEFSKADYVQELPISLDATETVPVLCRVSIVKSGH
                     DLNNSIHRPERCLPAQGHFNLTGTTVDVPVKGRGTIRMTKLKSQQNIAPDHPQPVILD
                     NMHYYVFIGHRHMTEDHLIRTLMDMKDRVLHGMDQRWCYFQISTTYGDKIRVPEEKAR
                     EQTERLISQLLSQLVKWDELNR"
     gene            complement(10923..11576)
                     /locus_tag="PK077_02485"
     CDS             complement(10923..11576)
                     /locus_tag="PK077_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRN23198.1"
                     /translation="MHFQRTLNRFSRENVPGYLAVFIGGFGDMFAGCLSRVEKGFPGF
                     LPGVAYATAYYHWDGGGCGVFFDRCGKIAVELEELRRRYPGLPVVLIGHSYGGSCAVE
                     VARRQSVQAAPLCLLTIDAVARRQKSVRPACAEWWGNAYLRDGGGFMDAVPRIGGRWG
                     HCAGADANLAFSGYSRDRKGRLYSHRRPAPMLYEPPGEEACSLFQAAASWLERNLPD"
     gene            11669..12253
                     /locus_tag="PK077_02490"
     CDS             11669..12253
                     /locus_tag="PK077_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HRN23199.1"
                     /translation="MQVIIHQWAFNGSFDQLASLPRQELVNDWFGYDVEPSAEFAFAT
                     DGEFLWFLASRSKAATIHPEARPGQFQPELWRYDLAEWFMAAGDGTNYWEFNLAPNGA
                     WWACAFSDTRRANEDIPAPLAVDTKCILTDEEWCAMARIPLNELRGVDIRDCKLAATF
                     ILDTPDQIFLTTADDLSGNPDFHRPDCFCTPILK"
     gene            12257..>13430
                     /locus_tag="PK077_02495"
     CDS             12257..>13430
                     /locus_tag="PK077_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRN23200.1"
                     /translation="MSLKEDISRILGPGKVSGAPDVLTAHAGDKWYAVVFPESTEDVS
                     LLLRYASSMNIPVTARGGGVGYVGGCVPVRGGISLSLERMNRILEISPEDGVAVVEPG
                     VITADLQREARALGWFYPPDPASRKECSIGGNIATNAGGPRCLKYGVTRAYVLGLTVV
                     LASGEVVECGGRTHKNKTGFDIGDLFIGSEGMLGIITRAVLRLIPHPEAFGALSAGFP
                     QFPLAAAAVQRILNSGHLPSALEITDSFTLRAARNYLGDSALPSGCRGHLIVEVDGRR
                     PAVREELDSLCTLLEECGATGILRADTEAEAEAIWQLRREFSYSLKATGMTKLNEDIV
                     IPRSRLVALVEFCEALNRETGLDIACFGHSGDGNIHTNIMVDDYADPEKKALADACV"
BASE COUNT         2927 a         4062 c         3590 g         2800 t
ORIGIN      
        1 atttccggca gaagaataac atcagcactc cctgcgatgc cgccttccaa ggcaatccat
       61 cccgcatgcc gtcccatcac ttccaccacc atcatgcgct ggtgcgcgtt tgcagtggtt
      121 tccagacggt ccaaagactc ggaaacaatg gaaacggcgc tgtagaagcc aaaagtgaca
      181 tcggttgcgc ccaggtcatt atcaatggtt tttggcacgc caactacggg gagccctatc
      241 tcggaaagca aaagagccgt ggactgggag ccgtctccgc ccacaacgat cagggcatcc
      301 agtcccagcc ggcggacagt agccttcgtt ttttccagca ccgcgggttc aatcgccccc
      361 ttttggtcca cgcctacttt gatggcgaag tttcctttgt tcacggttcc cagaatggtg
      421 ccccccttgg ctcgcaatcc gcggcaggcg gcaggggtca gccattcaaa ccgttcgtcc
      481 ccttcctcgg acaaaagccc ttcaaaaccg tcgtaaaaac ccacaacgtt ccagccgcga
      541 cgggaagccg cgcccacaac accttcaata actgcattca acccggggga gtcacccccg
      601 ctattcaaaa caccgatgtt catattcggc gttccttata gcagaaaaat ccttccccgt
      661 ctacttccta tctcctttgt ggagcagatt tcacaggaag ctggacaaat tccttggggt
      721 ccttcatatc catgccggaa gcctgccagt tttcccaaac gcgccagccg gtgttcatca
      781 tctccctggc gagggaatag cggctcgcag aaccaagcac cgcaataatc atgcgctgcg
      841 gataaatcac ctccgtcccc gtttgggggc tccggcggga aatagcgttg cgcgtcacgc
      901 tgagcagcag gcaggggccg gcggcgcggg aagtgcccgc cttgatgccg tccacgccgg
      961 aagcgctcat gagcctgttg ttgttggaaa tatcgtacat cgtcctgccc ttggtctggg
     1021 actgcacttc aatgcgccgg ctcgcctggg aaacaatgta gcaaaaagcc ggattttgca
     1081 tgctgtaaac gcctaacaag gccatatcca gcgcgcagga agtggaaacg gagttgccgg
     1141 cgtccagccc gtggggagcg gtaaagcgcg ttttggccat cctcagggaa cgggccaaat
     1201 tgttcatttc cgccacaaaa gcggcaatgg gggcgccgcc gccccggcgg gacaccaggt
     1261 cacgccccac aaaggaggca acagtcaggg cggaaacatt gtccgaaccc agcagggtgg
     1321 aatacaaagc gtcccggagc gtcaggcggt ctcccggctg gagatccatg ggattgcccg
     1381 cacggaggcc gaacgcttcc tgaggcacgg caatgaaagt atccagaggc aaccgcgtgc
     1441 gggccaccca gtccaaagcc accatggcgg tggctacttg ggaaagactg gccacgggcc
     1501 ttttttcttc tgcgttggaa ctgaaaagaa gcttgccgga attggcctca atggcaatat
     1561 aactgggccg ttcctgtgcc tgggcggaaa actggaatac acaggccagg gtcaaaagga
     1621 atagagatag ctttttcatg gcgggaccgg cgggatagtg cccgttccct cctcatttga
     1681 aaagtctaaa atgttttccg tacaacactt atatgtcatg acacggaaaa acgcattgac
     1741 ctttgggggc ccgtcctatt tgttggaaga agagtcttcc caaacggaag cgttcctgac
     1801 atacccagct ccatgaccag gattcagctt ttttacagcc gggcctaccc gggaaacacc
     1861 ctgcgcgcca ataccctcca ggcattggcc catctgggtg tcaatccgga gatgcaggtt
     1921 gaggagaccg ttccggaaca gatcgggacg ccgcttcccg cactcgccat tgacggggaa
     1981 atcgtcgtct atggcgtgga accttccgtg cacgagctgg aactccttct ccaagaccga
     2041 gtacaggagc gggaagcgga ttttccctgc ggcgcgtgcg gcatgggatg cagcggcatg
     2101 aacagcccgg aaaactgccc gatgtgcatc caggacggga aagacggcag catggcgggc
     2161 agaatcatcg gctttgccat cctgctcatg ctcctgttct tcgcgatgaa aatcctgtcc
     2221 tgaattgtcc tggatcttct catgccgggc tgcctctttt tacttacgct tccttcctct
     2281 ttcaggaaat gaagatacac ggcagttaac ggcgtttgcc cgcccacgca ggcggtcttg
     2341 ataatttctt gaattccccc ctcctccggg acacagtaga ggcatgcgct ccttcttcgg
     2401 ttccattctc ctgattctgg tgctggcagc cctcggcgcc accatttatt accattggtc
     2461 caccaactcc cacctggaat tcgaactccg caaccccgac gaaccggaaa aaacgataca
     2521 ggtcatcaac atggaaaaac gggcggcagc cctgcaggct gttccggggg aaaaaactcc
     2581 agtttccccc gcggaagaaa ttcccgcttc cgctgccgga atgccggaac ccgcggaatt
     2641 ataatccctc ctgttgccgc actatgaaac cggtcattgg atacacgctg ggcgaccagt
     2701 cgggcatagg cccggaagtc atctccgccg cgcttgcctc cggagaactc ccggaagacg
     2761 cagagtaccg cctcatcggc aagagagtgg agcttccccc gggaaggcct aatgcggaat
     2821 ccgccaaaca cgccttcgac catctggaac aggcggccca tgccctgcgg gaaggaacgg
     2881 ttgacgctgt cgtcaccgcc cccgtctgca aggaaaccct gcatgaggca ggtttccgct
     2941 ggccggggca gacggaattc tttgcagaac gcctggaaac ggacaattac gccatgtgcc
     3001 tgaccgggaa acggttgacg gtggggctgg caaccatcca tacttccctc caaagcgtgc
     3061 cttccctgct ggaaacggag gaactggtcc gcattggaac cctgctgaaa gacttctgca
     3121 tccgcaaggg aatcatgcgc ccgcgtatcg ccctggcagc cctgaacccc cacgccgggg
     3181 aacacggcgc tttcggcgat gaggacggga acatcattgc cccagccgtg ggacggctga
     3241 gggcgatcca tccggatgct gcatttgacg ggccttccgt cccggactgc gtgtaccggg
     3301 atgccgtgga agggaattat gacgccgtgc tggcccctta ccatgaccag gggctgattc
     3361 cgctgaagct ggtggacttc cataccgccg tcaacgtcac cctgggcctt ccccgccccc
     3421 ggctgagccc ggaccatggt accgccttca acctggcggg aaccggaaag gccaatccat
     3481 ccagcaccat ccacgctttc cagctggccg tacagatggc gcagacgcgt taacgttttt
     3541 cttctttccg gagctcttcc cgccataccc tgcgcaagac aaagctgttc cggaggggaa
     3601 caaagccctt cagcccgtgg cagcaaccgc tccagcggat ctgcctcccg ccagttcctc
     3661 cctttttttg caggaaaagg aatcctttac ccgggaaaag ctcatatcct aagttgcggc
     3721 gtccactctt ttccgtacgg cacccgccaa gcgaacgccg gaatcgtctc tcctacagca
     3781 aaggccgccg gcaggaaacg ccatgcagaa caattcttcc gcgccgtggg gagagtaaaa
     3841 cgcaggaaac ggcatgttcc cggaaacaag gtaaaaaata ggcttgcttt ctgaagacaa
     3901 tcattgcacc ttacttgccc gcaaggtgcg caccgttcgc acgacacatt tctaattatc
     3961 acaagaccat gagaaattac gaagctctta tcgtattcaa catgaagggt acggaaactc
     4021 ccgttgaaga gttgatcagc accgtagccg ccgcgatgaa ggaagaaggc gccgacatca
     4081 ccgccacgga aaacgccggc cgccgcgaat tcgcctacga atccaaccac ctttccgccg
     4141 ggcagtatgt gacctacacg ttctctgcgg aaccttccgc catcaggccc atccgcgaac
     4201 gccttcgcct gaatccccag attcaccttc agtattacaa ggtgctcggc taaaatccgg
     4261 cccatgcgga cgaaacatcc gctccttgct ttcttatcaa aaagaaccgt ctccgtgaag
     4321 gggcggttct ttttttatag attaccatcc ctttccccct ctgccccccc gctcaacaaa
     4381 tattccccgc cccttaagca atattgacca ggaaccggcg gtaagaaata aatttgccac
     4441 tccgcttgcc ctggctattt tccatacagg gaaaaactga gccatggagt tctacggagg
     4501 tgccgtccga agggtttcaa tgaattaatc ccggtgtctt tttaataagg ctgcttttcg
     4561 agaatgacgt ttcatttgat tcaccgctcc tccttgcccc ttgctgtcag ggaaaagagc
     4621 ggcagtctgt gagccggcaa tttccggctc acagggaaat aaagtttttc agcagtttca
     4681 cgcccagttt ctggcttttt tccgggtgga actgggtggc gaccagattg ccgcgcctga
     4741 cagcggcagc aaaacgcacg ccgtaggtgc agaaagcggc aatcagggat ttatcctccg
     4801 gctggggata ataggaatgc acgaaataaa agtacggttc cttccccatg cctttccaga
     4861 tgggatcgga gggatcagtg agagccacgc tgttccaccc catgtgaggc accttgagtt
     4921 ccgattctga aaaccgaacc acgcgcccct tgaggatgcc cagtccggga acctgcccgc
     4981 tttcttcccc gctttcaaaa agaacctgat agcccacgca gatgcccaga aaaggcctgt
     5041 cctgctctat ccatgcccgg accaggggca ccagattctg gcggcgcagc ttttccgcac
     5101 agtcgccgaa ggcgcccacg ccgggaagaa ccaaatgggt tacgcccgcc gcatcttccg
     5161 gagtacgcac cagatgaccg gtcacgccgg cggcctcaaa ggtgttcagc acactgcgga
     5221 gattgcccgc accgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5281 nnnnnncctg ccgcaacgcg tgggcgatgc ccttgaagac agattccgca atgtggtggc
     5341 catccctgcc gtaaagcact tccacatgca ggttgcaacg caggttattg gccagcgcac
     5401 ggcagaattc ctcgcaaagg gtcagcggaa agttgggggc atccggcaaa ccgccttccg
     5461 gaatacggaa agctacatac ggacggttgc tcaaatccat caccacgcgc gtaagagttt
     5521 catccatggg caggtaggaa cagccgtagc gaacaatgcc ctttttgtcc cccagtgcat
     5581 ttttaatgca ttcccccaga acgattccca catcctccac cgtgtggtgg gcgtctactt
     5641 ccaggtctcc gcgggcctga agggtcaggt ccatcaggga atggcgggcc agtaaatcca
     5701 gcatatggtc aaaaaacgca tgccccgttg cgacggcggc gcatcccgtg ccgtccaggt
     5761 tcagttccat gctgatgtcc gtttcacccg tgacccgttt gcaagaagcg ttcctcatgc
     5821 aacacttcta tactgaagac gcggcggaag aaagcaggaa atatcgcatg gggaaagatt
     5881 tgccctcttc tccccgcaaa aacatgcaga ccgtcaaagg aacacgcctc cggccccgct
     5941 ccgacagaaa acctcaggaa tctccgacaa tgaattgcgc gaaatcaacg gccggaaagc
     6001 ctgacgggca ggatggaatc aacacatact tgatccatcc catccgtaaa cgccatttcc
     6061 gctatatctc tccggagagt ttctccggaa aacggcagaa ttatttcttt ttggccttgg
     6121 cggctgcttc tctttcctcc tggcgcttct tatcccggtc ttcacgggct ttgcgcgccg
     6181 cttccgcacg ttcggcgcgt tcacgggcgc gctgttcccg caaccccacg cgtaccgttt
     6241 ccgtcagttc ctcatagtat tccctgggaa cctgggcggt ccggagttcc gtcagaacct
     6301 ccatagcatc gtcaaatttt ttctcgtcac acagattcca ggccttttcc agcgcagcgg
     6361 aaccggctcc gttttccggg tcctgttttt ttaagcgttc cgcatccgta gcggctacca
     6421 gcttaaggtc tctcatggct tccaaagaac ctgccgtagg ttcaaaccac cggtccccgc
     6481 gttccttgac cttcttgcgc aaagcccggt acacttccgt gagctggttt tcacgttccc
     6541 ggatggcagt ctgccttttc tggaaagcat ccatcaacac ctggcgctgt tccttggtca
     6601 gtttgggatt tccccggcgc ttctcttcct cctttctcag ggcttcacgt tccttctcca
     6661 aggacttttc cttggcggcg atcatgacct ccagcttacg gttgagctgt ccggcgattc
     6721 tggcggcatc cggataggca tccgccaggg cggcggaacc gggataaccg tcgcgcaagc
     6781 ggtcatacac ggccatggcc tggtatggat tgcgcacggc aagcgccttg aaacgcttca
     6841 gcagcttatt ggcctcatgg tcgtatttgg tgcgcacggt cacttccgcc tcctccctgg
     6901 cattctgggc ggcctgggct tcctccgtca atttcttttc ctccagcaaa accactactt
     6961 tggctcttgc cttgacggcg gcgtcatgag caaggccctg gggatttttc ttaatggtct
     7021 tgtccaggtc cgccaaggct ttttccaaat ccccggcatt catggtttcc ggtttggcat
     7081 acattttgcc gattcgggca gcctccagtt catccggagt cgccttcctg atggattcaa
     7141 tcaaatccct cttcacggtt acggaatcct tgatacctcc ggacacggga accaggaggt
     7201 tgacgctttc cggcgtttcc acttctaccg tgcaattttt gtaaacggtt ccatccttga
     7261 gttttacttc atctgcagct gcaagcggaa cgaatgcggt caacaccaat atgagggact
     7321 tgttcataaa tctaaggctt tttccagatt gatcaggcaa atacgtatcc tgcactttac
     7381 caaaagatgc cctgttggcg acaaaaaaaa gcccggagcc gcaaaaacgg ctccggaccc
     7441 ggtaaaagaa gaaacaggcg tatcaaactt atttctcggc aacagcttct tcagcagccg
     7501 gagccgcttc ctcgccggaa acacggggag cttccacaat agccacaatc acatcgccgg
     7561 ccagttccgt ggttacgccg gaggggagtt tgagatcggt aatgcgcagg ctttcaccca
     7621 gtttcaaacc ggaaatgtca gcggtaatgg cttccggcag atccttcacc tggcagataa
     7681 tcgccagttc gtgaatggtc tggtccagaa cgccgcccag ggccacgccg gcggaatcgc
     7741 cttccagaat gacgggaaca atggaatgga tgaccgtggt atcggtcaca gcctgaaagt
     7801 ccacatgcag gcaagcgttg gtgatggggt tgtgctgcac ttccttaagg agaaccttca
     7861 cgatcttgcc gttgatatcc agggccacga ggatgtgttc ggaaacagcg gaagccagca
     7921 tgcgggcaaa ttcacgggcg tcaacctgga tattgacgtt gtcaaaaccg ggaccgtaaa
     7981 ccacgccggg aaccaggccc tggctgcgga gttgcttcag attaccggag ccgcaggctc
     8041 tcgtttcggc cttgagggaa tgggatgtag ccatgtttta tagaggcaat attgagtgtt
     8101 gattgatgat gtttcctaat cgggttcagg cagaggcccc gccgggattt ccgaacccgc
     8161 gtaaaccggt actcaggtca gggcgcagga ctctgcacta aaattgtcta aatgtcaaat
     8221 aatgatgata cggattctcc gtcatgtatg cgctgcacgg catggcccag caggtctccc
     8281 acgctgacac agtccacttt ggggccatag gccatgggaa cggtgtcgga ggtaagaacg
     8341 cgttcaatgc aggaaccgga aatccgttcg cgggccatgt cccccagcac gccgtgagaa
     8401 acgcaggcga acacgcgctg ggctccccgt tccttcagga tattggcggc agcgcacagg
     8461 gtgccggcgg tctccgtcat gtcgtcaatc agaacggcat cgcgcccttc cacgtcacca
     8521 atgacattca tggcttccac atgggtggcg ttgacgcggt gcttggcaac aatggccaat
     8581 tccgcaccca gagcgtcgga atacgcgcga gccattttca cgccgcccac gtccggggag
     8641 acgacgacca gtttcttgag gtctttaaca taatgttcgc gcaggtgacg gattaaaacg
     8701 ggggcggcgt acaggtgatc caccggaata tcaaagaatc cctggatctg ggcggcatgg
     8761 agatccatag tcagcacgcg gtccactccg gcggccgtca gcagattggc caccagcttg
     8821 gcggtaatgg gaacgcgggg ctgatccttg cggtcctggc gggcataccc gaagaaagga
     8881 acaacagccg tgatgcgtcc ggcactggcg cggcgggcgg cgtccaccat cacgcacaat
     8941 tccatgatat tatggttagt gggggggcag gtgggctgaa tgaggaaaac atccttgccc
     9001 cggatgtttt catttatctt tacaaaactt tccccatcgg ggaaagtatt caccgtgacg
     9061 ttcgtcaact gaatgcctac gctttgcgca atgcgctccg caagttctct gtgtgctgta
     9121 ccgctaataa tcttcatacc ggaaactgaa aagcggacgc attctgaaca caggtccgca
     9181 taatcgcaat acttaattct cacaatttga tttttctcca tgatggattc ccccgctcct
     9241 gacggcaaag agagatttct gaccgccccc atgatcgccg ccttggccgt gagcacgctg
     9301 ctgctggcgc tgaactatct ggcattcccg gaattcggtt cattcggaaa cgaaaacagc
     9361 atccagtggc tcatttcctc ctggaacaag caaacggatt atgaacacgg ctggctggtg
     9421 gttcccatca tcgtcttcat gctgtaccat gccagaaaga aaattgccca ggcacccaga
     9481 cgcatggact ggcgcggcct gattcttttc atcccggcga tcatgcttct gatgctttcc
     9541 ttccgcgtgg ggcagcctcg cgtggccgta ggcgctcttc ccctgatttt attgggcggg
     9601 gcctggtacc tggccggacc gcagaccggc aggctgtgcg ccttcccgct gctctttttc
     9661 tggctgtgca tccccctgcc ctccttccag caggctacgg tggggcttca aatcattgcg
     9721 acggagctgg ggcattgggg ggcaaccatc ttcggggtgg acacctatct gcagggcacc
     9781 aatatccgct ctaccggcgg acactgggat gccttcaata ttgcaggagg gtgcagcggc
     9841 atgcgttccc tgatggcact gctcatgctg tccgcggcct gggcctacct gtccgacttg
     9901 aaattctgga aaaaatgcgt gctcttcctc agcgcgattc ccctggccgt catcggcaac
     9961 ggtgtccgca tcaccagcat cgtggtgatg gcggaatacg gaaatcccga gtttgcgtca
    10021 aaaacctggc atgactggtc cggcctgctg ttttttttcc ccatcagcct tttcggcctg
    10081 gcagccgtcc actccctgct ggccggggag ctcatctgga agccgtcaca gcgcaagaag
    10141 ctggttgtta aaatgaacaa gtcccattaa gcgtttccgc cgccatgaat attaaaacgc
    10201 tcaaaattct catcatccct tttctactgg ccgtcatgct cggcggcact tacctgatgc
    10261 cgcgcagggg ggaagtgcag gactcctcca tcagcatgga tctccccaca ggcatgaatc
    10321 cgtccggctg gcacggcacg cgccggcagg aatccgagca ggaacggggc atcctggccc
    10381 cggatacgga attttccaaa gcggattacg tgcaggaact ccccatcagc ctggatgcga
    10441 cggaaaccgt acccgttcta tgccgcgttt ccatcgttaa atccggtcat gatttaaaca
    10501 actccatcca ccgcccggaa cgctgcctcc cggcccaggg ccattttaac ctgacgggaa
    10561 ccacggtgga tgttcccgtg aaaggccgcg gaaccatcag gatgaccaag ctgaaatccc
    10621 agcagaacat cgctccggac catccgcagc cagtcattct ggacaacatg cattattacg
    10681 tatttatcgg ccaccgccac atgacggaag accacctgat ccgcaccctg atggatatga
    10741 aagacagggt tctgcacggc atggaccaac gctggtgcta tttccagatt tccaccacgt
    10801 acggggacaa gatcagagtg ccggaagaga aagccaggga acagacggaa cgccttatca
    10861 gccaacttct ttcccaactg gtgaagtggg atgaactgaa ccggtagcac ggctccggca
    10921 gttcagtccg gaagatttct ttccagccag gaggcagcag cttgaaaaag gctgcatgct
    10981 tcttccccag gcggttcata aagcatgggc gcaggacgcc gatgggaata aaggcggccc
    11041 ttccggtcac gactgtagcc ggaaaaagca agattggcat cagccccggc gcaatgtccc
    11101 cagcgtccgc ctattctggg caccgcgtcc atgaagccgc cgccgtcccg caggtaagcg
    11161 ttcccccacc attccgcaca ggccggacgc acgctctttt gcctgcgcgc cacggcgtct
    11221 atcgtcagaa ggcaaagggg agcagcctga accgactggc gcctggctac ttccaccgcg
    11281 caggagcctc cgtagctgtg tcctatcaac acgacaggca gcccgggata tctccgtctc
    11341 agctcttcca gctccaccgc tatttttccg cacctgtcaa aaaacacgcc gcagccgcct
    11401 ccgtcccagt ggtaataagc cgttgcgtac gccacacccg gcaaaaaacc gggaaatcct
    11461 ttctctacac ggctcaaaca acccgcaaac atgtccccga aaccgccgat gaacacagcc
    11521 agatacccgg gaacgttttc gcgggagaac cgattcagcg tcctttgaaa atgcatgacg
    11581 gcccccatga tggagaaatc acgtcaatat ccaagcctgt aagacgttct ggcttgataa
    11641 aacctgaaaa ctgtccaaat gtggtggtat gcaagtaatc attcaccaat gggccttcaa
    11701 cggaagcttt gaccagcttg cctccctgcc ccgccaggaa ctcgtcaacg actggttcgg
    11761 ctatgatgtg gagccaagtg cggaattcgc cttcgccacg gacggggaat tcctctggtt
    11821 tctggcttcc cgcagcaagg cagccaccat ccatccggaa gcccggcccg gccagttcca
    11881 gccggaacta tggcggtacg acctggcgga atggttcatg gccgcgggag acggcaccaa
    11941 ttactgggaa ttcaaccttg ctcccaacgg cgcatggtgg gcctgcgcct tttccgacac
    12001 ccgccgcgcc aatgaagata ttcccgctcc gctggccgtg gataccaagt gcattctgac
    12061 ggacgaagaa tggtgcgcca tggcccggat tccgttgaac gaactgcgcg gcgtggacat
    12121 ccgcgactgc aagctggcag ccacgttcat cctggatact ccggaccaga ttttcctgac
    12181 tacggcggac gacctttccg gcaatccgga tttccaccgc ccggactgct tctgcacccc
    12241 cattctcaaa taatccatgt ctttgaaaga agacatttcc cgcattctgg ggcctggaaa
    12301 agtttccgga gccccggatg tgctgacggc gcacgccggg gacaaatggt acgcagtcgt
    12361 tttcccggaa agcacggagg acgtctccct gctgctgcgc tacgcatcct ccatgaacat
    12421 ccccgtcacg gcccgcggcg gcggtgttgg atacgtaggg ggatgcgttc ccgtgcgcgg
    12481 aggcatcagc ctttccctgg aacggatgaa ccgtatcctg gagatatccc cggaagacgg
    12541 cgttgccgtg gtggaacccg gcgttatcac agcggatctc cagcgggaag cccgtgctct
    12601 gggatggttc tacccgccgg acccggcctc cagaaaggaa tgcagcatcg gaggaaatat
    12661 cgccacgaac gcgggcggcc ccagatgcct gaaatatgga gtcaccagag cttacgtgct
    12721 ggggttgacc gtggtgcttg ccagcggaga ggtagtggaa tgcggaggac gcacccataa
    12781 aaacaagacg gggttcgata tcggggacct ttttatcggt tcggaaggaa tgctgggcat
    12841 catcacgcgg gccgttttga ggctcatccc ccacccggaa gcattcggcg cgctcagcgc
    12901 cggcttcccg caattcccgc tggcagctgc cgccgtgcag cgcatcctga acagcgggca
    12961 tcttccctcc gccctggaaa taacagacag tttcactctc cgcgccgccc ggaactatct
    13021 gggggacagc gccctgcctt ccggctgccg cggccacctg attgtggaag tggacggcag
    13081 gcgacctgcc gtgcgggaag aactggattc cctatgcacc ctgctggaag aatgcggcgc
    13141 aaccggaatt ctgcgtgcag acacggaagc ggaggcggaa gccatctggc aactccgccg
    13201 ggaattttcc tacagcctga aagcaaccgg aatgaccaag ctcaacgaag acatcgtcat
    13261 tcccaggtcg cgcctggtgg ctctggtgga attctgcgag gccctgaacc gggaaacagg
    13321 gctggacatc gcgtgtttcg gccattccgg ggacggcaac atccatacca acatcatggt
    13381 ggacgattat gcggatccag agaagaaagc cctggcggac gcctgtgtgg
//
LOCUS       DAOMSU010000030        13304 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_207_length_13304_cov_3.056004, whole genome shotgun sequence.
ACCESSION   DAOMSU010000030 DAOMSU010000000
VERSION     DAOMSU010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13304)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13304)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13304
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_207_length_13304_cov_3.056004"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="PK077_02500"
     CDS             complement(<1..764)
                     /locus_tag="PK077_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23201.1"
                     /translation="MTFRSIFSLACCAAALLSPASAAPRPGDAKLRDDLDVAYNTWRL
                     HLLRGNYDGWRATTSAYRQVKVHNLAVSEKRPFPASLFQQPMAPPALAPLMYVGSVVN
                     GPTAAATYYGKVDLGLGQEPTDSNALVLLFTHENGKWKYDQARFFNLTRLPAVKERLK
                     RGDASVLMEQDGFQPLGKIPAVPPVCPPPKYIAKILVDCPGRTVQANVNNISLHEFDN
                     TRLAEVISGGLRDGTNSLTLNFSDSPNGKKGAVLVEV"
     gene            830..1705
                     /locus_tag="PK077_02505"
     CDS             830..1705
                     /locus_tag="PK077_02505"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRN23202.1"
                     /translation="MELFTEADHLAEARYRKLLREQPGFVWRRFSDGEELMAYVFFPP
                     GHRAEAAAPSVLFFHGGMWVGKSLGDFVPWALHLAQQGIAGIIPMFRTCGDYEVEPMD
                     ILEEGREAWAWVHGNAALLGLDPARITVAGSDAGGLMALHVALPDHSARWSLFRKKAP
                     LPPGPAAVALFRGVCDLTAQSAFKLGGMMPPDQRDAVNPLRRVQRGLPPLFASHGGRD
                     RLLPWRNSERLAELWRKKKNRSRFELLDVADHTFYHFNVNAAYFEQLLNSWSAFMVEQ
                     GIWEYNEGMDAGLLG"
     gene            complement(1764..2684)
                     /locus_tag="PK077_02510"
     CDS             complement(1764..2684)
                     /locus_tag="PK077_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23203.1"
                     /translation="MNPLYPLLAFSLLLPLCRHSMASDAVPTAIPAPKPETPRQAIEQ
                     FLGLTAIWMLEMEWTHAYANKTPKAEDFRNAMLAAGLQQCPADFQEAWLRQTARPGRN
                     YAAPVLRKYGVSLKDLRERLQGKLFKINPRVHPPIPLYDEGEQIPRMDPRTCGDPKAI
                     LEALAALRAQVMGLTPAQLSQARQSIERVMLEFTLAYMETAICMDTAGIRESQVRELF
                     APIRTEGCPEDFRRAWQHDLPFFLKGRFTGPELTLSAVCKKYGANEQELFLKVRRKMK
                     EWDIQPPTPQTQDAFRRDMREIRENMLGGR"
     gene            complement(2725..4740)
                     /locus_tag="PK077_02515"
     CDS             complement(2725..4740)
                     /locus_tag="PK077_02515"
                     /inference="COORDINATES: protein motif:HMM:NF017502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sip1-related alpha-galactosidase"
                     /protein_id="HRN23204.1"
                     /translation="MFPVLALAALSAWTSAAQPRTSAIPPISGKKAYRLPLPPYQTFC
                     WFSADRGRWPGDGNRVLPWFGRTSPGNLLDSRPNPSTAKPGDHAMFALFHLKDGNFMA
                     VLPVAAPDSLAWLKLERDGTFLVEAGSLGTSPAKPQAVLAVTATDKDIYRACSAVWNK
                     ALSLPFIKGRTLPREKKIYPEPFKYLGWCSWEQYKKNISSKLLEETAKKLEASPVPVR
                     WMLVDDGFQTQERLQLVSFQPRQDQFPRGWQPLMKHKSPKLKWMGLWHCYYGLWNGIH
                     PRHHLDDETARGLVRTAKGKILPGDGPGGAGAFYTPFLQSVKDAGFDFVKIDVQAEYL
                     KHADGLDNPVRHNTKCSEALEQACLKTGLSLVNCMAQGTVNIQNTRYSAVTRCSIDYK
                     LGDEAMAKSHILQSYANTLWLGQTVWPDHDMFHSTDPACARLMAVSKAVSGGPVYLSD
                     PADKLNPENIMPLVWSDGLLLRPLAPAVPLPDSVFPDALNENRLYRVIAPLPGQSAAV
                     VVYNLKHPSPAKPVRGKISLEDYKSAAALLNGNAAEAYASLPPEGIAAYSGEGGRALT
                     PAQPDLDVELTGFKDRLFIMAPIVQGWAVIGRRDKFLSPCALDSAPGYREDSLRFRVK
                     ESGPVVIWRGKGPVKVGNTPVRNLGNGFYELQFPVSNRPLDVTVTTE"
     gene            complement(4997..6115)
                     /locus_tag="PK077_02520"
     CDS             complement(4997..6115)
                     /locus_tag="PK077_02520"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRN23205.1"
                     /translation="MAYSSSPPSRSPRLHSLDALRGLDMLIILGLDALVLLLASRNPE
                     SAFLQEAARQMTHARWEGLHLYDLVFPVFVFISGVSMSFSLGKYTSGNASSVPGLLHL
                     WKRALVLAFLGLLVNGTLTWTESMRYASVLGLIGFSCAIAGTCILLCRRTGAVASAAG
                     FILALITLLQFTCGNFTPDGSVNSWVDRHWLAGRLHGGVFDPEGPLCVVSASVLCLGG
                     WLAGKFLKESRLSPVHLCAAMAGAGLLLFCAARMLDGLYPIIKSMWTGTFVLAAAGIS
                     LTLLSLFHLAVDVWNKGKWALWAFPLRVIGINALAAYLINSLLNIHALNRRVFSGIAD
                     FFTPFQPVFLAVSLLVLQWLVLYFLDRRRTSLAPKAAK"
     gene            6319..6849
                     /locus_tag="PK077_02525"
     CDS             6319..6849
                     /locus_tag="PK077_02525"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRN23206.1"
                     /translation="MKDGRYASSYEAFVADPQYRFTRDIWYHDERIQQARTRNSRIVI
                     HLNDQRGVLWVDGKPAMNFPVCTGKSAHETPRGKFSIIQKDADYRSRSYGSVFDASGL
                     CVNSDATSSSRVPPGGKFIGAKMPLWMRIHGGIGLHVGTVYRDANSHGCIRVPVEACR
                     ILFDKCGLGTAVIVQE"
     gene            6909..9377
                     /locus_tag="PK077_02530"
     CDS             6909..9377
                     /locus_tag="PK077_02530"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRN23207.1"
                     /translation="MKIKVLIRSLVLLAGLAAWTADTAWGQASTRRSSSRGGAAAGRA
                     AVEQRGEDDAVETVDLGDRKKDSDTASGEREGVAAAPEMPGETEKEQNGLDSLSLIPS
                     LGGGRLDAAREAHTSTRPSARTMALLIPAPRGPILDRNGEPLAVTSVAYQLALRFEIF
                     ENPDRSRVVEFGRNCLEQAKKIAGKAWSFSDEQLWKHYEHRRWLPLPLTNVIRAEEAE
                     KLKDKVKSVRGLQLLPIYIRSYPEKEIAGHIIGYVGSKGKLPTGPINHMDPLWEQVEG
                     RAGLEKEFNKNLTGTPGVWRLMFDEDGNKILDELQIRPKPGGAVVTTLNLKWQKDAER
                     ILSRGKRRGAMVVLDCVTGEVLVMASTPSYDPNMFIPNISQKDYDALRNDPAGPLGAR
                     AFQGRYPPASTFKVLTVASALRNNKITENTLVYCPASVTIGNHVFKNWSKTPLGDINC
                     VRALAMSNNPFMYQMGLKLGAEALMDTARAFGLGERTGLPIPDDPGLVPTSDYMIRNY
                     KRDFMSGDAANLSIGQGTLLVTPLQVAHMMSGVANGYLPRLQLIKQIQDGNANVVYAP
                     KPQEVQRPLPAYERALSSVRKGMREVVEAGTGGRARLSYSSIAGKSGTAQWGPEREDK
                     RLAWFAGFMPCDNPRFAYVVLYEGRAHERLGGGAAAAPIVKEFFETEKKDIKAIIDPP
                     KDDIPVAEPVEETPETAAAALRERSGAPAVVPDNLPPGLYDPEGMEPIKADEIPADLD
                     DSSDAEIKATPVGGSSGHAGPSAPPLSEEPSAAPLTPRRGDSDYIPGLPQQIPRHLNR
                     STPVNAPAPQSPLPEDDIPMAEPL"
     gene            9628..11187
                     /locus_tag="PK077_02535"
     CDS             9628..11187
                     /locus_tag="PK077_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23208.1"
                     /translation="MKKHYFYSIPCLALTCALAACSDDDKSSSAKAGQDTAGLTSAAS
                     PDPLTSVASDLVAKRAERLGIAALFPKDMGVVAGGYDIPGIMKSVQNLNMVKTWSSCS
                     SGEVADESIAIPGEDAPEPVQRKCSFSSPVIDAMIGLGPEWAPWLDSAQSSFTRMGLN
                     QMKEILSVYRLFQGVKNGSGDDDGAEAEAMGGQGVEMIAQWAELMDLRPSQAATAPVM
                     IAAKLTPEALAQAKAFLKDANLQEDMELHGMVSLYEKTYNGLACKVAEVDCKKVRAKL
                     EELLEEAKGQMDISQENMDRLKAALVRLDESRLYVAVSFVEDTLVGFITTNPEKQVRI
                     ASSPQDSVLSRPDFSMADARSQYPAYGLLFADKASVKGVINMDLAYYKGMFTGLKDVM
                     QAIGADWKIADPAPSMTALDSIRGSMLGMYEELARKATSVSYYSWQDQGVHVEACTYP
                     LEFYKLDAPSAMSGVRPGASTVLYSSCTVNPQMMDMGCSLCSNLAQIVWDYGNAYISN
                     PIHWKSPEGVV"
     assembly_gap    11229..11238
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11239..11930
                     /locus_tag="PK077_02540"
     CDS             <11239..11930
                     /locus_tag="PK077_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23209.1"
                     /translation="YISNPKHDVSDQVRMAAPMIQMARPTIEELWKAYKTALSGLTGS
                     GVFMMDMQGAPNPMLKNVPAPRFSAAYEVNNRAALGEAWQKVTHAAKTVVTLLSQGKM
                     AELPAPQSSVDGNITTYDYQCPLGADLNPVVSVSDTRWAISMPKAFGLEVLRESVKSP
                     AQGAPLEFQLNLVPVRDALKSAAEDSKKLRKTVETLDVITSDVTGVHATGRKAADGRD
                     VYHIHIIPAAK"
     assembly_gap    11980..11989
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12213..13151
                     /gene="atpB"
                     /locus_tag="PK077_02545"
     CDS             12213..13151
                     /gene="atpB"
                     /locus_tag="PK077_02545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRN23210.1"
                     /translation="MSRFFQFLWLAALTMFGVMGGSACAAEAGAEHGLEQAAPHLFSI
                     PLPGGIELPVSNSMLMLFLAVLLIGVVVWSATRSMRILPSRLQNAVEYFFETLYNFVK
                     SLLGPRLTRKYFWYFGTIFTIILVSNYMGLLPGVGTVTYHGVPLFRGANADMNVTMFL
                     GIFYALMWLYWSIREQGLKGFFMHLFGPKGRLPGFMGFVLVFIFIFVGLVDVLSITIR
                     PIALAARLYGNIYAGETIIDTMAHMFGPVLSALCVLPFLAIELLVGFIQALVFLLLTA
                     IFLKLQVGDDDAHGHTSKNEEKELPVPDSPPPGKAS"
BASE COUNT         2732 a         3606 c         4122 g         2824 t
ORIGIN      
        1 acttccacga gtacggcgcc ttttttgccg tttgggctgt cggaaaagtt cagtgtgagt
       61 gaattggttc cgtccctcag tcctccggaa atgacttccg ccaggcgggt gttgtcaaat
      121 tcatgcaggg aaatattgtt tacgttcgcc tggacggtgc ggccgggaca gtccaccaga
      181 atttttgcga tgtatttggg cggcgggcag acaggcggca cggcgggaat tttcccaagg
      241 ggctggaacc cgtcctgctc catcagcacg gaggcgtctc cgcgtttgag gcgttctttg
      301 acggcgggca ggcgggtgag gttgaaaaag cgcgcctggt catatttcca tttgccgttt
      361 tcatgggtga acagcagcac cagggcgttc gaatccgtgg gttcctggcc cagccccaga
      421 tccaccttgc cgtagtaggt ggctgcggcc gtggggccgt ttacgacgga acctacgtac
      481 atcagcggag cgagcgcggg gggagccatg ggctgctgga acagggaggc gggaaagggg
      541 cgtttttctg agacggccag attgtgcact ttcacctggc ggtaggcgga ggttgtggct
      601 ctccagccgt cataattgcc gcgcagcaga tgcagacgcc atgtattgta agccacgtcc
      661 aggtcgtctc tcagtttggc gtctccgggg cggggagctg cggaagcggg agaaagaagg
      721 gctgcggcgc agcaggccag ggagaaaatg gaacggaaag tcattacacg attatttaga
      781 catcaaactt gcgcggagtg caacacaatt gcatcatgaa gccgttctta tggaattgtt
      841 cacagaagcc gatcatttgg cggaagcccg gtacaggaaa ctgttgcggg aacagcccgg
      901 ctttgtgtgg aggcggtttt cagacgggga ggagctgatg gcgtacgtct tttttccccc
      961 cggccaccgg gcggaagccg ccgctccttc cgtgctcttt ttccacggcg gcatgtgggt
     1021 gggcaagagc ctgggggatt ttgtgccgtg ggcgcttcat cttgcccagc agggtattgc
     1081 aggcattatc cccatgttcc gcacgtgcgg cgattatgaa gtggagccga tggacattct
     1141 ggaagagggc cgggaagcct gggcctgggt gcatgggaac gccgcactgc tggggctgga
     1201 cccggctcgc attactgtgg cgggaagtga tgccggaggg ctgatggccc tgcatgtggc
     1261 ccttcctgac cactccgccc gctggtctct gttccgcaaa aaggctcccc ttcctcctgg
     1321 ccccgccgcc gtggccctgt tccgaggcgt gtgcgacctg acggcgcagt ccgcttttaa
     1381 gctgggcggc atgatgcctc cggaccagag ggatgccgtc aaccccctgc ggcgcgtgca
     1441 gagggggctt cctcccctgt ttgcgagtca tggaggaagg gacaggctgc tgccgtggcg
     1501 caacagcgaa aggctggcgg agctgtggag aaaaaagaag aaccgttccc gatttgaact
     1561 tctggacgtg gcggaccaca cattttacca tttcaatgtg aatgccgcct attttgaaca
     1621 actgctcaat agctggagcg cgtttatggt ggagcaggga atttgggaat ataacgaagg
     1681 aatggatgcc gggctgctgg ggtgaccttg gcctgatatc cgggaaggtt cccggaaggc
     1741 gctgcatgcg gaaaggctgt tttctatctg cctcccagca tgttttcccg gatttcccgc
     1801 atgtcccggc ggaaggcgtc ctgcgtctgt ggcgtaggcg gctggatatc ccattccttc
     1861 atttttcgtc gcaccttgag aaagagttcc tgctcgttgg ctccgtattt tttgcagacg
     1921 gcggagagag tcagctccgg ccccgtgaag cggcctttga gaaagaaagg cagatcgtgc
     1981 tgccaggcgc gccggaagtc ttccgggcat ccttctgttc tgatgggggc gaacagttcc
     2041 ctgacctggc tttcccggat gcccgcagta tccatgcaga tggcggtttc catgtaggcc
     2101 agcgtgaatt ccagcatgac tcgttcaatg gactgtctgg cttgggacag ctgggccggg
     2161 gtgaggccca tgacctgggc gcgcagggcg gccagggctt ccaggatagc cttggggtct
     2221 ccgcatgtgc gcgggtccat gcgtggaatt tgttcccctt cgtcgtaaag ggggatgggt
     2281 ggatgcaccc gagggttgat tttgaacagt ttgccctgga gcctttccct gaggtctttc
     2341 aggctgacgc cgtacttgcg caggacggga gccgcataat tgcgtcccgg cctggccgtt
     2401 tgccgcagcc atgcttcctg aaagtccgcg gggcattgct gaaggcccgc cgccagcatg
     2461 gcattccgga aatcctccgc cttgggcgtt ttattggcat aggcgtgggt ccattccatt
     2521 tccagcatcc agatggcggt aagacccagg aattgttcta tcgcctgcct gggagtctca
     2581 ggtttcggcg cgggaatggc agtcgggacc gcgtcggagg ccatggaatg ccggcagagc
     2641 gggagcaaca gggagaaagc aaggagggga tacagcgggt tcatggaaaa tgaagcaggg
     2701 aaaaatgaga ggaaatgtgc ggaattattc cgttgttaca gtaacgtcca gggggcggtt
     2761 ggagacgggg aattgcaatt cataaaaccc gttccccaga tttctgacgg gggtgtttcc
     2821 tactttgacc gggccttttc cccgccagat gacgaccggc ccggattcct tcacgcggaa
     2881 gcggaggctg tcctccctgt acccgggagc ggaatccagg gcgcagggag aaagaaattt
     2941 gtcccttctg ccaatgacgg cccagccttg gactataggg gccatgatga acaggcggtc
     3001 cttgaatccc gtcagctcta cgtccagatc cggctgtgcc ggagtcagcg cgcggcctcc
     3061 ctctccggaa taggcggcga tgccctccgg cgggagagag gcatacgctt ccgccgcatt
     3121 gccgttcagc agggccgccg cgcttttata atcctccagg gaaattttcc cccgtaccgg
     3181 tttcgccggg gaaggatgtt tgaggttgta caccaccacg gcggcgcttt gaccgggcag
     3241 aggggcgatt acccggtaca ggcggttctc gttcagagca tccggaaaga cggaatccgg
     3301 aaggggcacg gcgggagcca gcgggcgcag cagaagaccg tccgaccaga cgagaggcat
     3361 gatgttttcc ggattaagtt tgtctgccgg gtctgaaaga tagacagggc cgcctgagac
     3421 ggctttggat acggccatga ggcgggcgca ggcggggtct gtggaatgga acatgtcatg
     3481 gtccggccac acggtctgtc ccagccagag ggtattggcg taggactgga ggatatggga
     3541 tttggccatg gcttcatctc ccagcttgta gtcaatgctg caccgggtga ccgcgctgta
     3601 acgcgtgttc tgaatgttga cggtaccctg cgccatgcag ttgaccaggc tgagccctgt
     3661 tttcaggcag gcctgttcca gggcttccga gcacttggta ttgtggcgga ccgggttgtc
     3721 cagcccgtcc gcatgcttca ggtattccgc ctgcacatca attttcacga agtcgaatcc
     3781 ggcgtctttg acggattgca ggaagggggt ataaaacgct ccggctcctc cggggccgtc
     3841 accggggagg attttccctt tggcggtacg gaccagaccg cgggcagtct cgtcatccag
     3901 atggtgcctg gggtggatgc cgttccagag gccgtaatag cagtgccaca ggcccatcca
     3961 tttcaatttg gggcttttgt gtttcatcag gggctgccag ccccggggga attggtcctg
     4021 ccggggttgg aaactgacca gctgaagcct ttcctgggtc tggaatccgt cgtccaccag
     4081 catccaccgg acggggacgg gggatgcctc cagttttttg gccgtttctt caagcagctt
     4141 ggaggaaatg ttctttttat actgttccca gctgcaccag ccgaggtact tgaacggttc
     4201 cggataaatt ttcttttccc ggggcaaggt tctgcccttg atgaaaggga gggaaagggc
     4261 cttgttccat acggcggaac aggcgcggta aatatccttg tccgtggcgg tgacggccag
     4321 gacggcctgc ggtttggcgg gggaagttcc cagcgaaccg gcttccacaa ggaatgtccc
     4381 gtccctttca agtttcagcc acgcgaggga atcaggggcg gcgacgggga gcacggccat
     4441 gaaattgccg tctttgaggt ggaacagggc gaacatggca tggtcccccg gtttggcggt
     4501 gctgggattc ggtctgctgt ccagcaggtt gccgggggat gtccgtccga accagggaag
     4561 aacccggttc ccgtctccgg gccagcggcc gcgatccgct gaaaaccagc agaaggtctg
     4621 gtagggcggg agcggaagcc gatacgcctt tttgcccgat atgggaggaa tggcggatgt
     4681 ccggggttgg gcggcgctgg tccaagcgct cagggccgcc aaagcaagga ccgggaacat
     4741 gattgctttc atggaatgat cacagggttg gaaaatgggg caggggaacc cgggggaatt
     4801 gtggaaggaa cgtgctttgc ctctttcgga aaagatggca gagcttttcc ctgaaacgag
     4861 caaaaaatga tgtttccgct ttatttctct tttcagggcg ttgagggaaa gaaataacgt
     4921 cggccggccg tctcacaggg gaacttcgtt ttttcagaga acaccggcca ccctggccgg
     4981 gagttccgcc gcccggttac ttggccgctt ttggggcgag ggaagtgcgg cgcctgtcaa
     5041 ggaagtacaa gacgagccat tgcagcacca gcagggacac agccaggaag acgggttgga
     5101 aaggtgtgaa aaaatctgct ataccgctga agacgcgtcg gttaagtgca tgaatgttga
     5161 gaagagagtt aatgaggtag gcggccaggg cattgatgcc gataacgcgg aggggaaacg
     5221 cccatagggc ccatttccct ttgttccaca catcgacggc cagatgaaac agggaaagga
     5281 gcgtcaggga gatgccggcg gcggccagga cgaaggtgcc tgtccacatg cttttaatga
     5341 tggggtagag gccgtccagc atcctggcgg cgcagaaaag gagaaggcct gcccctgcca
     5401 ttgctgcgca gaggtgcacg ggagagagcc ggctttcctt caaaaatttc cctgccagcc
     5461 atcctcccag acacaggacg gaagcggaaa cgacgcagag cgggccttcc gggtcaaaaa
     5521 ctcccccatg cagacgaccg gcgagccagt gcctgtccac ccatgagttg acggagccgt
     5581 ccggcgtgaa gttgccgcag gtgaactgga gcagggtaat gagggccagg atgaaccccg
     5641 ccgcagaggc aaccgctccc gtccggcggc acagcaggat gcaggttccc gcaatggcgc
     5701 aggaaaaacc aatcagcccc agaacggagg cataacgcat gctttccgtc caggtaagcg
     5761 ttccattgac cagtagcccc agaaaagcca ggacgagagc ccgtttccag agatggagaa
     5821 gcccggggac ggaggaagcg tttccgctgg tgtatttccc cagggaaaag gacatggaaa
     5881 cgccggaaat gaagacgaaa acggggaata ccaggtcgta caggtgcaat ccttcccagc
     5941 gggcatgcgt catctggcgt gcggcttcct gaagaaaagc gctttccgga tttctggaag
     6001 ccaggagcag aaccagggcg tccaggccca ggataatgag catgtccagc ccgcgcaggg
     6061 cgtccaggga gtgcaggcgg ggagaacggg agggggggga agaggaatag gccatttaaa
     6121 agcatcatac ggaaaaaaaa ggagtttcta tcaggattcc tccaaacatg aaggaacgga
     6181 ggggaatcgt gcttgatttc cacgggggaa gaaggtcgca tgccgcacac ccttcccgct
     6241 tttctttttt ttctgtgtgt ctccctgttt ttgtccgctt gctcctctac ccccaggacg
     6301 cctcacggaa agccgttgat gaaggatggc aggtatgcgt cttcctatga agcttttgtc
     6361 gcggaccccc agtaccgctt cacgcgcgat atctggtatc atgacgaacg catccagcag
     6421 gcgcggacgc ggaacagcag aatcgtcatt catctgaatg atcagcgggg cgtgttgtgg
     6481 gttgacggca agccggccat gaactttccc gtttgcacgg gcaaatccgc ccatgaaacg
     6541 ccgcgcggca agttttccat catccaaaaa gatgcggatt accgttcccg gtcttacggc
     6601 agcgtgtttg acgcaagcgg cctgtgcgtg aattcggacg ctacctcttc ttcccgcgtg
     6661 ccgcccggcg gtaaattcat cggcgccaaa atgccgctgt ggatgcgcat tcatggcggg
     6721 atagggctgc atgtgggcac cgtgtaccgg gatgccaact cccatgggtg catccgggtt
     6781 ccggtagagg catgccgcat cctgtttgac aaatgcggcc tgggaacagc cgtcatcgtt
     6841 caggaataag cgtgagcgcg aataatcgtt gattccttgc ggtatatttg ccacactcct
     6901 gcaaactgat gaaaataaag gttctgatac gttccctggt attgttggcc gggttggccg
     6961 cgtggacggc ggatacggcc tggggccagg cttccacgcg ccgttcgtct tctcgggggg
     7021 gagcggctgc tggccgggct gcggtggagc agcgcggaga ggatgacgct gtggaaacgg
     7081 ttgacctggg agacaggaaa aaggattccg atacggcctc cggagaacgg gaaggcgtgg
     7141 ccgccgctcc ggagatgccc ggggaaacgg aaaaggaaca gaatggcttg gattccctgt
     7201 ccctgattcc ttccctcggc gggggacggt tggacgccgc gcgggaagcg cacaccagca
     7261 cgcgcccttc cgccaggacc atggctttgc tgattcccgc gccgcgcggc cccatcctgg
     7321 accgcaacgg ggaaccgctg gccgtgacgt ccgtagccta tcagctggct ttgcggtttg
     7381 aaatttttga aaaccccgac cgttcccgcg tagtggaatt cgggcgcaac tgcttggaac
     7441 aggccaaaaa aattgccgga aaggcgtggt ctttttccga cgaacagctc tggaaacatt
     7501 atgagcaccg ccgctggctg cctcttccgc tcacgaacgt cattcgtgcg gaggaagcgg
     7561 aaaagctgaa ggataaagtg aaaagcgtgc gcggccttca gctgttgccc atttacatcc
     7621 gttcctatcc ggagaaggaa atcgccggcc atatcatcgg ctatgtggga tccaagggaa
     7681 aactgccaac cggccccatt aaccacatgg acccgctgtg ggagcaggtg gaaggccgtg
     7741 cggggctgga aaaggaattc aataaaaatc tgaccggaac gcccggcgta tggcgtttga
     7801 tgtttgatga agacggcaat aaaattctgg atgaactgca aatacgcccc aagcccggcg
     7861 gagctgtcgt caccaccctc aacctgaaat ggcagaagga tgcggaacgc atcctttccc
     7921 gcggcaagag gcgcggcgcc atggtggtgc tggattgcgt gacgggtgaa gttctggtga
     7981 tggcgtccac gccgtcctat gacccgaaca tgttcattcc aaacatatcc cagaaggatt
     8041 acgacgccct gcgcaacgat cctgccggtc ctctgggcgc ccgtgctttc cagggccgtt
     8101 atcctcccgc ttccacattt aaagtcctga cggtagccag tgccttgagg aacaacaaaa
     8161 tcacggaaaa tacgctcgtt tactgcccgg cttccgtgac gatcggcaac cacgttttca
     8221 aaaactggag caagactccc ctgggggata tcaactgtgt ccgcgccctg gcgatgtcca
     8281 acaatccgtt catgtaccag atgggcctga agctgggtgc ggaagccctg atggatacgg
     8341 cccgtgcttt cggcctgggg gaacggaccg gcctgcccat tccggacgat cccggcctgg
     8401 tgccgaccag tgattatatg attcgcaatt acaagcgcga tttcatgtcc ggggatgccg
     8461 ccaatttgtc catcggccaa gggacgctgc tggttacgcc cctccaggtg gcgcacatga
     8521 tgtccggcgt ggcaaacggg tatctgccca gactccagct catcaagcaa attcaggacg
     8581 gcaacgccaa tgtcgtttac gcgcccaagc cccaggaagt tcagcgcccg ctgcctgcct
     8641 atgagcgtgc tctgtccagc gtgcgcaagg ggatgaggga agtggttgaa gccggtacgg
     8701 gggggcgtgc gcgcctgtcc tattccagta tcgccggcaa atcaggaacg gcccaatggg
     8761 ggccggaacg ggaagacaag cgtctggcgt ggtttgccgg tttcatgccc tgcgacaatc
     8821 cgcgctttgc ctatgtggtg ctgtatgaag gccgcgccca cgaacggctg ggcgggggag
     8881 ccgccgccgc tcccatcgtc aaggagtttt tcgaaacgga gaaaaaagac atcaaggcta
     8941 ttattgaccc gcccaaggac gacattccgg tggcggaacc ggtggaggaa accccggaaa
     9001 cggccgcggc cgctcttcgg gaacggagcg gggcccctgc cgtcgtgccg gacaacctgc
     9061 cccccgggtt gtatgatccg gaagggatgg agccgatcaa ggctgatgaa atccctgccg
     9121 atctggatga ctcttccgat gcggaaatca aggcaacccc ggtgggcggc tcctcagggc
     9181 atgccgggcc gtcggcgccc ccgttgtcgg aggagccttc tgcggccccc ctcacgccgc
     9241 gccggggaga ctccgattac attcccgggt tgccccagca gattccccgc catctcaacc
     9301 gctccactcc cgtaaacgct cccgcgccgc agtctcccct gccggaggat gatattccca
     9361 tggcggaacc cctttaatcc gtttttcagg gaaatccgga gcgtttttgc ggttcccgga
     9421 aaatcccggg aagatgcgga aaggggcggc gcggaaaggt tctccatgcg tcaacgccct
     9481 gcgcgccctg agtggcgtta atgctttgca ttgtgcttgc gtctcgcggg aaaacgcctg
     9541 tataatgaag acggtgcgct gatgagactg ttgccgcaag gagcggcgtt cctgtcagat
     9601 gacatcatga ccccacgttt aaagataatg aaaaagcatt atttctactc cataccctgt
     9661 ttggcgctga cctgcgctct tgccgcctgc tctgacgatg ataaatcatc ttccgccaag
     9721 gccggccagg atacggccgg cctgacttcc gccgcttcgc ctgatccttt gacttccgtt
     9781 gcttccgatc tggtggcaaa gcgtgccgaa cgcctcggaa tagccgccct ctttcccaag
     9841 gatatggggg tggtagcggg gggttacgat atccccggca tcatgaagag cgttcagaat
     9901 ttgaacatgg ttaaaacctg gagttcctgt tcatccgggg aggtggccga tgaatccata
     9961 gccattccgg gagaagacgc tcccgaaccc gtgcagcgga aatgttcttt cagcagtcct
    10021 gtcattgacg cgatgatcgg cttgggcccc gaatgggctc cgtggctgga tagcgcgcag
    10081 tcctctttta ccaggatggg cctgaaccag atgaaggaaa ttttatccgt ttacaggctt
    10141 ttccagggag taaagaatgg aagcggggat gacgatggcg cggaagcgga ggccatgggc
    10201 ggccaggggg tcgagatgat tgcgcagtgg gctgagctga tggatttacg cccttcccag
    10261 gctgcgacgg cgcccgtgat gattgccgcc aaactgacgc cggaagccct ggcccaggcc
    10321 aaagctttcc tgaaggacgc caatcttcag gaggatatgg agctgcatgg aatggtttcc
    10381 ctgtacgaaa agacctacaa tggcctggcc tgcaaggtgg cggaagtgga ctgcaaaaag
    10441 gtgcgcgcga agctggaaga gcttctggag gaggcaaagg ggcagatgga tatttcccag
    10501 gaaaatatgg accgcctcaa ggcggcgctc gttcgtctgg atgaatccag gctgtatgta
    10561 gccgtctctt ttgtggagga taccctggta ggctttataa ccaccaatcc tgaaaaacag
    10621 gtccgcatag cctcttcccc ccaggactcc gtgctgtctc gtcctgattt ttccatggcc
    10681 gatgcccggt cccagtatcc ggcctacggt ctgctcttcg cagacaaagc ctccgtcaaa
    10741 ggggttatta acatggatct ggcctattac aagggcatgt tcaccgggtt gaaggacgtg
    10801 atgcaggcaa taggcgccga ttggaagatt gccgatccgg ctccttccat gacggctctg
    10861 gattccatca ggggcagcat gcttggcatg tatgaagagc tggcccggaa ggccacgtcc
    10921 gtttcctact attcatggca ggatcagggg gtgcatgtgg aagcatgcac atatccgctt
    10981 gaattttaca agctggacgc cccctccgcc atgagcggcg tgcgtccggg cgcgtccaca
    11041 gtgttgtatt cctcctgcac ggtcaatccg cagatgatgg atatgggttg ttccctgtgc
    11101 agtaaccttg cccagattgt atgggactac ggcaatgcct atatttccaa cccgattcat
    11161 tggaagtcgc ctgagggagt agtatagaaa gatcagctat aaacccggcc acattctttt
    11221 gagccccgnn nnnnnnnncc tatatttcca acccgaaaca tgatgtgagc gatcaggtgc
    11281 gcatggccgc ccctatgatc cagatggcca ggcctaccat tgaagagctc tggaaggctt
    11341 ataagactgc tctttccggc cttacgggtt caggcgtatt catgatggac atgcagggcg
    11401 ctcccaatcc catgctcaag aatgtgccgg cgccgcgctt ctccgctgcg tatgaagtga
    11461 acaaccgcgc cgctctgggc gaggcctggc agaaagtgac tcatgccgcc aaaaccgtcg
    11521 tcaccctgct cagccagggt aaaatggcgg agcttcccgc tccccagtct tctgtggacg
    11581 ggaatatcac tacctacgat taccaatgcc ctctgggcgc cgatctgaac ccggtagtaa
    11641 gcgtttccga cacccgctgg gcaatcagca tgccgaaggc tttcggcctg gaagtcctca
    11701 gggaatccgt gaaatctccg gcgcagggag cccctctcga atttcagctt aacctggttc
    11761 cggtccgtga cgccctgaag agcgcggcgg aggacagcaa gaagctccgg aagactgtgg
    11821 aaaccctgga tgtaatcact tccgatgtta cgggagtgca tgccacgggg cgcaaggccg
    11881 cggacggcag ggatgtgtac cacatccata ttattcccgc cgccaagtaa cggccttcct
    11941 ccgcccagtt tttcctgcag gcttccggga gtttattccn nnnnnnnnng aagccttttt
    12001 tgttgatgga gaaaaggttg tgttcatgac gcaacggtca tccaggtagg ctttaaaatt
    12061 atgtacaaat gtatacaatt tgcgtacgcg ccaagttttg ggcttgccaa aagaaagagg
    12121 gaagtacata atcaaaaccg tcaaataaag ggaatggatt ccctcttcca gtatttacag
    12181 gagttggcac aagtaatggt gagagaaaga caatgagcag atttttccag ttcctgtggt
    12241 tggcggcgtt gaccatgttc ggcgttatgg gcggcagcgc ctgtgcggcg gaagcaggag
    12301 cggagcacgg attggaacag gctgctcccc atttgttttc cattccgctg cccggaggca
    12361 ttgagctccc ggtgtccaac tcaatgctga tgctgtttct ggcggttctt ctgatagggg
    12421 tggtcgtatg gtccgccacc cgttccatgc gcatcctgcc ttcccgtttg cagaatgcgg
    12481 tggaatactt ttttgagact ctgtacaact ttgtgaagtc ccttctgggg ccgcgcctga
    12541 cacgcaagta cttctggtac ttcgggacca ttttcaccat tattctggtc agcaattaca
    12601 tggggcttct tcccggggtg ggaaccgtca cttaccatgg ggtgccgctg ttccgcggcg
    12661 cgaatgcgga catgaacgtg acgatgttcc tgggtatttt ttatgcgctg atgtggcttt
    12721 actggtctat ccgggagcag ggactgaaag gcttctttat gcatctgttc ggccccaagg
    12781 gaaggctgcc ggggttcatg ggatttgtac tggtgttcat cttcatcttt gtggggctgg
    12841 tggacgtcct gagcatcacg atccgcccca ttgcgctggc agcccgtctt tacggcaata
    12901 tttacgccgg ggagacgatt attgacacta tggctcacat gttcgggccc gtgctcagcg
    12961 ccctgtgcgt actgccgttt ctggcgattg aactgcttgt ggggttcatt caggccctgg
    13021 ttttcctgct cctgacggcg attttcctga aacttcaggt aggggacgat gacgcccacg
    13081 gccatacctc caaaaatgag gaaaaggaac ttcccgttcc ggacagcccg cctcccggaa
    13141 aagcctcata aaccgtaccg ccaactttcc gttccggacc atgggcgacc tgtgggggaa
    13201 cgttcaacaa aaaacataaa ccataccaat aacatgattg atcctatcgc aatgacctcc
    13261 ctggccgaac tgtccggcaa cgtaggtttc ggcctcgtca ccat
//
LOCUS       DAOMSU010000031        13199 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_213_length_13199_cov_2.907562, whole genome shotgun sequence.
ACCESSION   DAOMSU010000031 DAOMSU010000000
VERSION     DAOMSU010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13199)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13199
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_213_length_13199_cov_2.907562"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..1092)
                     /locus_tag="PK077_02550"
     CDS             complement(70..1092)
                     /locus_tag="PK077_02550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HRN23211.1"
                     /translation="MSPKHLANPLHGILLIVLFSFSAFYIADFEWVKHLSLSPLIVGI
                     VLGMLYANSLRNHLPETWVPGIQFCTKQVLRTGIVLYGFKLTFQSVIDIGGSALALDL
                     IVVTLTILLGAGLGRLLKMDRDTALLTSIGSSICGAAAVLGAEPVVKSKPYKAAVAVS
                     TVVIFGTLSMFLYPALHRAGILDLTPEQMGLFTGATLHEVAHVVGAGNAMGQAISDPA
                     IIVKMIRVMMLAPVLVVLSIVLARRDPSSGANGEKRKITIPWFAFLFLAVIGFNSLHL
                     LPAGLVDAINTLDTFLLTMAMTALGAESSFEKFKKAGARPFLLAGFLYIWLFFGGYWL
                     VKLISL"
     gene            complement(1211..>1830)
                     /locus_tag="PK077_02555"
     CDS             complement(1211..>1830)
                     /locus_tag="PK077_02555"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HRN23212.1"
                     /translation="LSLGASSTMSQYVLPPLLALFNRHYPEIRIWLKSGNTEEIEQAV
                     QDESVQLGIVEGTATRADLHYQPFMEDEIILAGGMQAAVPSEGLSLPQLSHVPLIMRE
                     PGSGTRRVVEEALKKHGISVSGLHVLMMLGNTESIKMYLEHSPACSFLSSLAVRGEFK
                     RGSLKKIHLRHLEIRRTFHFVSGHGDHSRINELFIKFCRYYYNKI"
     assembly_gap    1831..1870
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1871..2102)
                     /locus_tag="PK077_02560"
     CDS             complement(<1871..2102)
                     /locus_tag="PK077_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRN23213.1"
                     /translation="MVEYYFLSMITDFRLKVFETVARRLNFTRAAEELFITQPAVTRH
                     IKELERLLDARLFKREGRGIALTAEGVRLLAHS"
     gene            2253..2672
                     /locus_tag="PK077_02565"
     CDS             2253..2672
                     /locus_tag="PK077_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23214.1"
                     /translation="MKLHSMIIAGGAGLLLLGACSPSSYQTTFPEPAYVKVSTNNGAY
                     APYPDDPVGQQELVYWYNSLAAPMTVVAEVPPTPIVTTGSGPVVLDSSDFSAKLYRDR
                     TEIKKKDKDGNWVTSVRPTTPADQRMRNYVMASKKAR"
     gene            complement(2669..3076)
                     /locus_tag="PK077_02570"
     CDS             complement(2669..3076)
                     /locus_tag="PK077_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23215.1"
                     /translation="MCSALLVAAVWLLHPEWPFWAGYIAWALAYAATLIVQHTIPLSI
                     RCPHCGNAGLYSAPQTPEEQRRPDTGIMLECRGCGSTFYTDAYLPWPGKIIRRRPTCP
                     EEWEEKSARKQAGPASSGEERRPERSFVNGGKI"
     gene            complement(3162..4262)
                     /locus_tag="PK077_02575"
     CDS             complement(3162..4262)
                     /locus_tag="PK077_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HRN23216.1"
                     /translation="MKSLLALCLFYSAASVSTAETPIKHTSFRPGEIWTDNNGAHINA
                     HGGGILYDKGTYYWYGEHKVEGDAGNYAQVGVHCYSSKDLYNWKDEGIALKVVEGDHS
                     HPIAKGCILERPKVVYNKKTGKYVMWFHLELKGKGYGSAYAGVATAAKPTEPFKFLKA
                     GRVNPGKWPLNMDKKDQTTEFTKEMPDYVRIIRRDYPGGQMSRDMTIFVDDNGKAYHI
                     YSSEGNITLHIAELTPDYTGHTGKFVRAFPGRFMEAPAIFKHKGKYYLIASGCTGWAP
                     NAARSAVAKNIAGPWMELKNPCVGPKAGITFGGQSTFILPVQGKPGKFIFMADIWRPK
                     NAIDGRYLWLPMKWEGDQIILEWKDEWTLDDL"
     gene            complement(4271..5524)
                     /locus_tag="PK077_02580"
     CDS             complement(4271..5524)
                     /locus_tag="PK077_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRN23217.1"
                     /translation="MFRPLTTIFLATLCLHLPAAQGESVPEEKTDVIPIAKIPDISPA
                     KPGQFDRAFRRGVDFLLKTQNKDGSWGDHRVIGTWNILCPYPDGPLTFKTASTALCIA
                     GLNASPLHHEPAVQEAMTRAEDYLIRTMHHLKRGDALCVYNTWAHTYVLDAMSMRAAR
                     LAPDSLRYRELKECARSQVKKLNELASAMGGWGYLTYSGFSKRPAAQPTSFLTGTVLI
                     SAWMAGKSFGLSLDDKIFTRALKFLKSQRTPAGTYVYSLSHSFYPGRPINRHTGSLAR
                     TPACDYAIRLWEPEDISLRQLVDGLDRLWSRRGWLTMALHKPTPHESFAQNSGYFFYY
                     GYYYSGMCLDMLAPNQVKRHASLLADDILTRQGTDGAWWDYPLYNYHKFYGTGYALYA
                     LSRAWDKLYGQPEPSPSSISTLSRP"
     gene            complement(5528..6754)
                     /locus_tag="PK077_02585"
     CDS             complement(5528..6754)
                     /locus_tag="PK077_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRN23218.1"
                     /translation="MNKAVLFLAALGASTALCAPVPAEKAEKRDTIPIARVPDVPPPS
                     RETLDASIRKAADFLLKEQNRDGSWGNHTRTKGLNVLCPYPEGPRSFRTASTSLCVIG
                     LLTSPLKEDPAVKASLDKALQYLLTTLPTLKRGDTRTVLGVWGHAYGLSALSRAARNL
                     PADAPLREELKKVASLQVKALDQMADVQGGWGYYTFETFSKRPIGEPTSFLTATVLLA
                     YKDAEQAFGLKAEPRVVKRAVACLEKLRTPAGSYVYSLSHSFYPGRPINRHTGSLART
                     PAGDLALMRYDPSFISRSQLEDGLERIWSRGGWLSLAVKKPIPHESFAQNAGYFFYYG
                     YYYAARCLEEVAPEHLARHAAHLASDLLPMQEEDGSWWDYPLYNYHKFYGTGYALYAV
                     SRVRESLYPSDAATTS"
     gene            complement(6774..7958)
                     /locus_tag="PK077_02590"
     CDS             complement(6774..7958)
                     /locus_tag="PK077_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23219.1"
                     /translation="MNCTSLTMAGLLAALSLGIPSLADSIAPGILLPKKNTKSISFSP
                     KGTSDIFLLGIMPQGSSVKKGESVAQADFRILDNSIEDYERAIKSKNLEVLKLRYALD
                     QQKERSALALQKYQTALTRTEEDQKDFLEKRKARMLAEEEERVNKALRHMSYKQEELN
                     QLTKMYKDDQVAEETEEIILKRLKNELGESEFAVQGAKLVAELAKLRNIHRLGEDYAT
                     AVKEKQMDLEQARKQADFDLEQKKIALTEAEVSLRRLQNKYNELKADREMASFKAPEN
                     GILLYGGYVADKWVAIPVAEKLKPGGKLGAFDKIATIVPPDSELIVQATLPDSTATPK
                     VGETVVIKITNMQIPGVITEASPIPGADGKRRIIITPKVPASQIFAPGLPVQVTIKDQ
                     QA"
     gene            complement(7974..8654)
                     /locus_tag="PK077_02595"
     CDS             complement(7974..8654)
                     /locus_tag="PK077_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23220.1"
                     /translation="MKPITKLASSTLPDGSVLDLWERDGFHFLLRDGVQTASSFSHGS
                     NEAAAAMAAAPVKRANQPSFLLDGLGLGFTLFALMDQIRREKARFIVAEPCKPLIDWH
                     KELMNQERPGFLHDPRVSVEFTPALQIARKNPKSFHAILSHSTHERMNLSAAEASDYF
                     AALKQGGLLVITVARPDTRLSRTLQKAGFEVSTEAVPASHKGKKTAFHTVILGKKGRF
                     VPFSAHQS"
     gene            8843..9532
                     /locus_tag="PK077_02600"
     CDS             8843..9532
                     /locus_tag="PK077_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23221.1"
                     /translation="MSNPPPPSIPGGTPPPPSIPTPPAAPSVPKAPAAPAVPPVAAVP
                     AAPAAPPAAAAPTIPLRPAGLGGGIKLGGGLKTVALGSATTPVAPVVPPAGAAPAAAA
                     PTVPLTPPGAGMATAQLPQATIGLNTAPAASPVSSASFTARKFQEDTDAAPSTLLNVL
                     SFVALAFALVFIGYQYATDTLEARSMVGWLGGDSKPAAAAPAADEETVDDVSSETSAE
                     EPSDDGADAEE"
     gene            9571..9888
                     /gene="trxA"
                     /locus_tag="PK077_02605"
     CDS             9571..9888
                     /gene="trxA"
                     /locus_tag="PK077_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRN23222.1"
                     /translation="MANVFSEANFEDEVLKSDIPVLVDFWATWCGPCRMIAPIIDQLS
                     TELAGKIKVGKVDVDANNGLAATYGVRTIPTLLIIKDGEIMDTMVGASSKDAIMQRLR
                     PFM"
     gene            10188..11393
                     /locus_tag="PK077_02610"
     CDS             10188..11393
                     /locus_tag="PK077_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRN23223.1"
                     /translation="MAPDLKSGAFWKLPQPDLFGKYFNGEPGGWVDKGKTQLRIAKPS
                     IKIGDMSLGEMLVNWKDGAPQSMTVMMYNKGDNGAIGKDEFDKRLELIKESLTALTGI
                     QPKEYRASRKEAVVKVNGWSWIWDNGAITLEINTSREGREFEAEFIRMKAGPTEDSIA
                     RGDASSRARKADIKQHVRKEGKRVVIQDIPMVDQGQKGYCVVATAARIFAYYGMDYVD
                     QHELASLANTSADGGTNTAAMAENLKKIGTRFQIRIKVLDSLANSRDFRNLLKAYNRA
                     ASKLKKEKVENEHDWSGFWDNADGEVLKLARAGSPSQVDRWLNAIKPYIMAGIPVFWS
                     VQLGIVPEPLRLSQTRGGHLRLIIGFDEEKKTLIFSDSWGAAHTEKEMPLADAIAITT
                     GRQVMQPSK"
     gene            11469..11621
                     /locus_tag="PK077_02615"
     CDS             11469..11621
                     /locus_tag="PK077_02615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23224.1"
                     /translation="MGKALYYTCAMMNRTLVKHVLDSEVEMPDVLIQGWVRTRRDSKT
                     FSFLEV"
     assembly_gap    11651..11660
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11661..12929
                     /gene="asnS"
                     /locus_tag="PK077_02620"
     CDS             <11661..12929
                     /gene="asnS"
                     /locus_tag="PK077_02620"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRN23225.1"
                     /translation="FSFLEVNDGSSVASLQVVADEGIPGYERIGEMATGSAVSIRGAL
                     VAGQGRQRWELRAASLELVGAVPDSYPLQKKGHTPEFLRSIAHLRPRTNLFGAVFRMR
                     SRLAFAIHRFFQERDFSWVSTPIITASDCEGAGEMFRVTTLDVGDAASRDASRDFFGK
                     AAYLTVSGQLEGEAFACALSNIYTFGPTFRAENSNTTRHAAEFWMVEPEMAFCDLYGD
                     MDMAEEFVRFLVGDALANSAEEVEFLTRFVDKGLWERLEHVKETPFVRCSYTEAVDIL
                     LKSGKTFDYPVSWGINLQSEHERFLTEEHFKCPVIVYNYPKEIKPFYMRLNEDGKTVT
                     AMDVLVPGIGEIVGGSQREERLDILEENMRRMGMNAEAYRWYADLRRYGTVPHAGFGA
                     GFERLLMFVTGVTNIRDVLPFARTPRNCEF"
BASE COUNT         2752 a         3432 c         3938 g         3027 t
ORIGIN      
        1 tttttctcct gttcggaaag gcagggcgta ggccttgccc tctttccgca ggaggaatgt
       61 ttttgaaatt tagaggctga tcagcttgac gagccagtaa cctccgaaga aaagccagat
      121 atacaggaag cctgcaagca ggaaaggccg cgctccggct ttcttaaact tctcaaagct
      181 ggattccgcg cccagggccg tcatcgccat ggtcagcagg aatgtatcca gggtgtttat
      241 ggcgtccaca agtccggcag gcagcaggtg cagggaatta aagccaatga cggcaaggaa
      301 caggaaggcg aaccagggaa tggtaatttt ccgtttttcc ccgtttgcgc cggaagaggg
      361 atccctccgg gccaggacaa tgctcaggac gaccagcacc ggggccagca tcattacccg
      421 gatcattttg acgataatgg ccggatcgga aatagcttgc cccatggcgt tgccggcgcc
      481 caccacatgc gccacttcat gaagggtggc tccggtaaaa agccccattt gttccggcgt
      541 cagatccagt attccggccc ggtgcagggc tggatacaga aacatggaga gcgtgccgaa
      601 gatgaccacg gtggaaacgg ccacagcggc tttgtaaggt ttgcttttga cgacgggttc
      661 cgcccccaga acggctgctg ccccgcaaat ggaactgccg atggaggtca gcagggccgt
      721 gtccctgtcc attttcagca ggcgccccag cccggctccc agaagaatgg tgagagtgac
      781 cacaataagg tcgagcgcca gggcggagcc gccgatgtcg atgacacttt gaaaagtcag
      841 tttgaatccg tacaggacaa tgcctgttct caaaacctgc ttggtgcaga actgaatgcc
      901 gggaacccat gtttccggaa ggtggttgcg caggctgttg gcgtaaagca ttcccagcac
      961 gatgccgacg atcagcgggc tgagggaaag atgtttcacc cattcaaaat cagcaatgta
     1021 aaaagcggaa aaggaaaaca gaacaataag caggatgccg tggaggggat tggctagatg
     1081 tttgggagac atggtcggta aatgatggtt gcggctgccc gttgtatctg gccgggagcg
     1141 taccggattg gctgcggaat aatatacgat gcagccatta taaaatctaa ttaaaaattg
     1201 ttatagccaa ttaaattttg ttataataat atcggcagaa cttgatgaac agttcattga
     1261 ttctgctgtg gtctccatgt cctgagacga agtggaaggt gcggcgaatt tccaggtggc
     1321 ggagatgtat tttcttcaaa cttccgcgtt tgaattctcc ccggacagca agggaggaga
     1381 gaaaagagca cgccggagaa tgctccagat acattttgat gctttccgta ttgcccagca
     1441 tcatcagaac atggaggccg gaaacggaga tcccgtgttt tttcagcgct tcctccacca
     1501 cccgacgcgt gccggacccc ggttcacgca tgatcagggg cacatgggag agttgcggga
     1561 gggaaagccc ttcactgggc acggcggcct gcatgcctcc tgccagaata atttcgtcct
     1621 ccataaatgg ctggtaatgc aggtccgcgc gcgttgccgt gccttccacg atgccaagct
     1681 gcacggattc atcctggacg gcctgttcta tttcctccgt attgccgctt ttcagccata
     1741 tgcgtatttc cgggtaatgg cggttgaaca gggccagcag cggaggcagc acatactggg
     1801 acatggtgga gctggctccc aggctcaatt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn gggaatgggc cagcaggcgc acgccttccg ccgtcagggc aatccctcgt
     1921 ccctcccttt tgaacagacg ggcatccagc agacgttcca gttccttgat atgccgggtg
     1981 acggccggct gcgtgatgaa cagttcttcc gccgcgcgcg taaaattcag gcgccgtgcg
     2041 acggtttcaa acactttcag ccggaaatcc gtaatcatgg agaggaaata atattcaacc
     2101 atgccgccgg ggggacggca agctcctttc ctccgcatga tggcgtacgg cgctgggaat
     2161 ggcgctcaag cgcaagacgc gcgggtgccc gtaatattcc ggaaccgtac atcctctgaa
     2221 cggcaggtgg cggaagagag tccaatgcct atatgaaact gcattccatg attattgccg
     2281 gcggagccgg ccttttgttg ttgggggcct gttccccctc ttcctatcag accacatttc
     2341 cggaaccggc ctatgtaaag gtttccacca ataacggagc ttatgctcct tatccggacg
     2401 atcctgtcgg ccagcaggaa ctggtgtact ggtataattc ccttgccgct ccaatgacgg
     2461 tggttgcgga ggttcctccc actcccattg tcacgacagg ttcggggccg gttgttcttg
     2521 attccagcga tttctccgcg aaattgtacc gcgacagaac ggaaatcaaa aagaaggata
     2581 aggacggcaa ttgggttacc agcgtgcgcc ccaccacccc cgcagaccag cgcatgagga
     2641 attacgtgat ggcttccaaa aaagcccgtt agatttttcc tccgttgacg aaagaccttt
     2701 ccggcctgcg ttcctctccg gaggaagcgg gcccggcttg tttacgggca gatttttctt
     2761 cccattcttc cgggcaagtg gggcgccggc ggataatttt tcccggccag ggcaaatagg
     2821 cgtccgtgta aaaggtggaa ccgcaacccc ggcattccag catgatgcct gtatccggcc
     2881 ttctttgttc ttccggagtt tgcggagcag aataaagccc ggcgtttccg cagtgcgggc
     2941 agcggatgga aaggggaatg gtgtgttgaa caatcagcgt tgccgcgtaa gcgagtgccc
     3001 aggcaatgta gcccgcccag aaaggccatt ccggatgaag cagccagact gctgcaacaa
     3061 ggagagcgga gcacaaggca tacagaaggc tccgcttctg ccttccggca aggagatgga
     3121 agcaataaac cgtatcctgc ataaaaagga aggagatgaa tttacaggtc atccagcgtc
     3181 cattcatcct tccattccag gataatctgg tcaccctccc atttcatggg cagccacagg
     3241 taacggccgt caatggcgtt tttgggccgc cagatgtccg ccatgaagat gaattttccg
     3301 ggtttgccct gtacaggcag gatgaaagtg ctctggccgc cgaacgtgat gcctgccttg
     3361 ggccccacgc agggattttt caattccatc catgggccgg cgatgttttt ggccacggcg
     3421 ctgcgggcgg cgttgggagc ccagcccgtg catccggaag cgataagata gtatttgccc
     3481 ttgtgtttga agatggcggg agcttccatg aagcgtccgg gaaatgcccg gacgaatttt
     3541 cccgtatgcc ccgtgtaatc cggcgtcagc tccgcgatgt gaagggtgat attgccttcc
     3601 gaggaataaa tgtggtaggc ttttccattg tcgtccacaa aaatggtcat gtcccggctc
     3661 atctgcccgc cgggatagtc cctgcggatg atgcggacgt agtcgggcat ttccttggtg
     3721 aattcggtcg tctgatcctt tttatccatg ttcaggggcc atttgcccgg attgactcgc
     3781 ccggctttca ggaacttgaa tggctctgtg ggttttgctg ccgttgccac ccccgcgtaa
     3841 gcggatccat atcctttccc cttgagttcc aggtggaacc acatcacgta ctttccggtc
     3901 tttttgttgt aaaccacttt ggggcgttcc aggatgcagc ccttggcgat ggggtgggaa
     3961 tggtccccct ccaccacctt cagggcgatt ccttcgtcct tccagttata gaggtctttg
     4021 gaggaatagc agtgaacccc tacctgggca tagtttcctg cgtccccctc caccttatgt
     4081 tctccgtacc agtaataggt tcccttgtcg tacaggatgc cgccgccgtg ggcgttgatg
     4141 tgcgcgccgt tgttgtccgt ccagatttct ccgggtctga aggaagtatg tttaattggg
     4201 gtttctgcgg tggagacgga agccgcactg taaaacaggc agagcgccaa aagcgatttc
     4261 atggtattta tcatggccgg gatagtgtgg aaatagagga tggagaaggt tccggttggc
     4321 cgtacagctt gtcccaggcg cgggaaagag cgtacagggc atatccggtg ccgtagaatt
     4381 tgtggtaatt atacaggggg tagtcccacc aggccccgtc tgttccctgg cgggtgagaa
     4441 tatcgtccgc caacagggat gcatgacgct ttacctggtt tggcgccagc atgtccaggc
     4501 acattcctga ataatagtat ccgtaataga aaaaatagcc ggaattctgg gcgaaactct
     4561 cgtggggagt tggtttatgc agggccatcg tcagccatcc cctgcggctc cacaggcggt
     4621 ccaggccgtc cacaagctgg cgcagggaga tgtcctccgg ttcccagagc ctgatggcgt
     4681 aatcacaggc gggagtccgg gccaggctgc cggtatggcg gttgatgggc ctgccgggat
     4741 agaaggaatg ggaaagggaa taaacatagg ttcccgcagg cgtcctctgg gatttgagga
     4801 atttcagcgc gcgggtgaat attttatcat caagggaaag gccgaatgat tttcctgcca
     4861 tccaggcgga gataaggacg gttcccgtca ggaaagaggt gggctgtgcc gcagggcgtt
     4921 tgctgaagcc ggaataagtg aggtagcccc agccccccat ggcggaagcc agttcattaa
     4981 gtttttttac ctgggagcgg gcgcattcct tcagttccct gtagcgaagg ctgtccgggg
     5041 ccagtcttgc agccctcatg ctcatggcat ccagcacgta agtatgcgcc caggtgttgt
     5101 aaacgcacag agcgtcgccg cgtttcagat ggtgcatcgt acggattaga tagtcttcag
     5161 cacgggtcat ggcttcctgc acggcgggtt catgatgcag ggggctggcg ttgagccctg
     5221 caatgcacag ggcggtactg gctgttttga aggtgagggg cccgtccggg taggggcaga
     5281 ggatgttcca ggtgccgatt acccggtggt cgccccagga gccgtcctta ttttgcgttt
     5341 tcagcaggaa atccacgccg cggcggaatg ccctgtcaaa ttgtccgggt tttgcagggg
     5401 aaatatccgg gattttggcg attggaataa catccgtttt ttcctctggc acggattccc
     5461 cctgcgctgc gggaagatgc aggcacaagg tggcgagaaa aatggtcgtc agcgggcgga
     5521 acatgggtca tgaagttgtt gccgcatcag agggatacag ggattctctg acgcgggaga
     5581 cggcatataa ggcatatccg gtgccgtaga atttgtggta attgtacagg gggtaatccc
     5641 accaggaccc gtcttcttcc tgcatgggca ggaggtcgct tgccagatgg gccgcatggc
     5701 gcgccaggtg ttccggcgcc acttcctcca ggcagcgggc ggcgtaataa tacccgtaat
     5761 agaaaaaata gcccgcgttc tgggcaaagc tttcatgggg aatgggcttc tttacagcca
     5821 gcgaaagcca gccgccgcgg ctccagatgc gttccaggcc gtcttccagc tgactcctgg
     5881 aaataaagga cgggtcatac cgcatcagag ccagatcccc ggcgggagtg cgggccaggc
     5941 tgccggtgtg gcggttgatg ggcctgccgg gataaaagga atgggaaagg gaatagacgt
     6001 agctgccggc gggcgtcctc agtttttcca ggcaggcgac ggcgcgtttg acgacgcggg
     6061 gttccgcttt gaggccgaag gcctgttccg cgtccttgta ggcaagaagg actgtagccg
     6121 tcaggaagga agtgggttct ccaatgggac gcttggaaaa agtttcaaat gtgtaatagc
     6181 cccagccgcc ttggacgtcc gccatctggt ccagcgcttt gacttggagg gaggccacct
     6241 ttttgagttc ttcgcgcaaa ggggcatccg caggaaggtt ccgcgccgcc cggctcaggg
     6301 cggagaggcc gtaggcatgg ccccatacgc caagcacggt acgggtgtct ccccgtttca
     6361 gggtgggcag cgtggttagc aggtactgca gcgccttgtc cagggatgcc ttgacggcag
     6421 ggtcttcttt cagggggctg gtcaggaggc cgatcacgca caaagaggtg gaggctgtcc
     6481 tgaatgatct ggggccttcc ggataagggc agagaacatt cagacccttg gtgcgcgtat
     6541 ggtttcccca ggaaccgtcc ctgttctgtt ctttcaacag aaagtcagcc gccttccgga
     6601 tagaagcgtc cagagtttcc cgggagggag gcggaacatc cggaaccctg gcaatgggaa
     6661 tggtatccct tttttccgct ttttccgccg gaacaggagc gcagagcgcc gtactggctc
     6721 caagggcggc aaggaaaagg acggctttat tcatggggat ccgttaaggg aaatcaggcc
     6781 tgttggtcct tgatggtcac ctggacggga aggccggggg cgaaaatctg gctggcggga
     6841 accttgggcg tgatgataat gcggcgcttg ccgtctgctc ccggaatggg gctggcttcc
     6901 gtaataacgc cgggaatctg catgttcgtt attttgatga cgacggtttc ccccaccttg
     6961 ggcgtagcgg tggagtccgg aagagtagcc tggacaatca gttccgagtc tggcggaaca
     7021 atggtggcga ttttgtcaaa agctcccagt ttgccgccgg gtttcagctt ttccgccacc
     7081 ggaatggcca cccacttgtc cgccacatat ccgccgtaca gcaggatgcc gttttccggg
     7141 gccttgaaag aggccatttc ccggtctgct ttcagttcat tgtacttatt ctgaaggcgt
     7201 ctcagggaga cttccgcctc cgtcagggct atctttttct gttccagatc aaaatccgcc
     7261 tgcttccttg cctgttccag atccatctgc ttttccttga ctgccgtagc atagtcttcc
     7321 cccaggcgat gaatattgcg cagcttggcc agttccgcaa cgagtttggc tccctgaacg
     7381 gcaaattcgc tttcaccaag ttcatttttc aggcgtttca aaatgatctc ttccgtttct
     7441 tccgccacct ggtcatcctt gtacatcttg gtaagctggt tcagctcttc ctgtttgtag
     7501 gacatgtggc gcagggcttt gttgacgcgt tcctcttctt ccgccagcat gcgggccttc
     7561 cgcttttcca gaaaatcctt ttgatcctct tccgtccgtg tcagggccgt ctgatatttt
     7621 tgcagggcca gggcggaacg ttccttctgc tggtccaggg cgtaccggag tttcagcacc
     7681 tccaggttct tgctcttgat ggcgcgttca taatcttcga tactattgtc cagtatgcgg
     7741 aagtccgcct gggctacgga ttcccctttc ttgacggaac ttccctgcgg catgatgccg
     7801 agcaggaaaa tgtccgaggt ccccttgggg gagaaggaaa tggatttggt attttttttg
     7861 ggaagaagaa tgcccggggc aatggaatcc gccagggagg gaattcccag ggacagggcg
     7921 gccagaagtc cggccatggt aagggatgtg cagttcataa taataggatg gaatcaggac
     7981 tggtgagcgg agaatgggac gaaacgtccc ttctttccaa gaataacggt gtgaaaagcg
     8041 gttttctttc ctttatgtga ggccggaacg gcttccgtgc ttacttcgaa ccccgctttt
     8101 tgcagtgtcc gggaaagtcg ggtgtccggg cgggctaccg taatgaccag cagaccgccc
     8161 tgcttgagcg cggcaaagta gtcggaagct tcggctgcgc tcaggttcat gcgttcgtgc
     8221 gtggaatggg acagaatggc gtggaaactt ttcggattct tgcgggcgat ttggagcgcc
     8281 ggggtaaatt ccacgctgac gcggggatcg tgcaggaatc cgggccgttc ctggttcatc
     8341 agttccttgt gccagtcgat aagcggtttg cagggttccg ccacgatgaa gcgggccttt
     8401 tccctgcgaa tttgatccat cagggcgaac agggtgaatc ccaggccaag gccgtcgaga
     8461 aggaaggagg gttggttggc tcttttaacc ggagccgccg ccatggctgc cgccgcttca
     8521 ttgcttccgt gggaaaagct ggacgctgtc tgtacgccgt cgcgcaaaag aaagtggaaa
     8581 ccgtcccgtt cccataggtc gagaacggag ccgtcgggca gggtggagga agctagtttg
     8641 gtaatgggtt tcatatgaga taatgccgga tacacagcgt aggaaagaac gcttttcagg
     8701 gcaagtccgg acagcgtgtt tgagatggaa tacgcttcaa attaaaaaaa attgcacgga
     8761 attcaggttt tgcgcattct ttttcttgat ttacgggctg aaataagcga gagctatcgt
     8821 tcagcacagt tatttttccg atatgagtaa tccacctccc ccctccattc cgggcggtac
     8881 tccgcctccg cccagcattc cgactccgcc ggctgctcct tccgtgccca aggcacctgc
     8941 cgctccggcc gttcctccgg tagccgccgt gcctgcggca cctgctgctc cgcctgccgc
     9001 cgctgcgccc actattcccc ttcgtcccgc cggtcttggc ggaggcatca agcttggcgg
     9061 aggattaaag acggttgccc tcggctccgc cactacgcct gtggcccccg tggttcctcc
     9121 tgctggcgcg gcgcctgccg ctgccgctcc tactgttcct ctgactcctc ccggagcagg
     9181 catggcgaca gcacagcttc cgcaggccac catcgggctg aataccgcac ccgcagcctc
     9241 tccggtttct tccgcctcct ttacggccag gaagtttcaa gaagatacgg atgcggctcc
     9301 cagtacgctg ctgaatgttc tctcctttgt ggcccttgcc tttgcgcttg ttttcatcgg
     9361 ttatcaatac gctacggaca ccttggaagc ccgcagcatg gttggctggc tgggcggaga
     9421 ctccaagcct gccgccgctg ctcccgccgc cgatgaagaa acggtcgatg acgtttcttc
     9481 cgaaacttct gccgaagagc cttccgatga tggcgccgat gcggaagaat aagtgaacgt
     9541 aatgattatt gaaatactct aagcggtact atggcaaacg tattttctga agcaaatttt
     9601 gaggatgaag ttctgaagtc cgacattcct gtgcttgtcg atttctgggc tacatggtgc
     9661 gggccctgcc gcatgatcgc cccgattatt gaccagcttt ctacggaatt ggccggtaag
     9721 atcaaagtgg gcaaagtgga tgtggacgcc aataacggac tggccgccac ctatggtgtg
     9781 cgcactatcc ccactcttct gattatcaag gacggggaaa tcatggatac gatggtggga
     9841 gcttcttcca aggacgctat catgcagcgt ttgcgtccgt tcatgtaaat aatcggactg
     9901 gtttattcat taagccggcc tgctgcgggt ttactgcggc aggccggttt ttttaccggg
     9961 cagcattatt agaaaaggac ggatgggaga ggataaagaa ggaatggttc gctggaggaa
    10021 gattcatttt ttcttggcag aaccgtcgtg ggagaataca ttaccagcgt agattccgag
    10081 cagcggggct attccccgcc ccttttttcg ttatgcgatt tatctctgcc attctgcctt
    10141 tcctttcagt ctgctgcctt cttccctccg cttggggcgc gggcaacatg gcgccggatt
    10201 taaagtctgg tgcgttctgg aagctgcctc agccggattt gttcggaaag tattttaacg
    10261 gagagcccgg cggctgggtg gataaaggca agacccagct ccggattgcg aagccctcca
    10321 tcaagatagg ggacatgtcc ctgggggaaa tgctggtgaa ctggaaggac ggagcccccc
    10381 agtcaatgac tgtcatgatg tacaacaagg gggataacgg ggccattggc aaggatgaat
    10441 ttgacaagcg cctggagctc atcaaggaat ccctgaccgc tcttaccggg attcagccca
    10501 aggagtaccg ggcctcccgt aaggaggcgg tagtcaaagt caacggctgg tcctggattt
    10561 gggacaacgg ggcgattacc ttggagatca atacatcgcg ggagggcagg gagtttgaag
    10621 cggaatttat ccggatgaag gccggtccca cggaagattc catcgcgagg ggagatgctt
    10681 cttcccgggc cagaaaggcg gatatcaagc agcacgtcag gaaagaagga aagcgggtgg
    10741 tgattcagga tattcccatg gtggaccagg gccagaaagg ttattgcgtg gtggctaccg
    10801 ccgcccgtat ttttgcctat tatggaatgg attatgtgga tcagcatgaa ctggcttccc
    10861 tggccaatac ctctgcggac ggaggcacta atacggcggc aatggcggaa aacctgaaaa
    10921 aaatcgggac gcgcttccag atacggatca aagtgctgga ttccctggcg aattcccgtg
    10981 atttcaggaa tctgctgaaa gcctataacc gcgccgcctc caagctgaaa aaggagaagg
    11041 tggaaaatga acatgactgg tccggtttct gggacaatgc ggacggagaa gtcctgaagc
    11101 tggcgcgtgc cggatcaccg tcccaggtgg acaggtggct caatgccatc aagccctata
    11161 tcatggcggg catccccgtg ttctggtccg tgcagcttgg cattgttccg gaaccgctgc
    11221 gtttgtccca gacgcgcgga ggccatttga ggctgattat cggttttgac gaggaaaaga
    11281 aaacgcttat cttttcagat tcctgggggg ccgcccatac ggagaaggaa atgccgctgg
    11341 cagatgccat cgccattacg acggggcggc aggtcatgca gccttccaaa taacaggtgc
    11401 ggcatatccc ctccttctct gccggaaaat ccggaatatt ctctccgtgg tgaagcaatt
    11461 ctgctggcat gggcaaggcg ctgtattata cttgcgccat gatgaacaga acattggtga
    11521 aacatgtcct ggacagtgag gtggaaatgc cggacgtact gattcaggga tgggtgcgta
    11581 cgcgccgtga ttccaagact ttttcctttc tggaggtgta gtcgaggtat tgaaacgcca
    11641 ccgtctgccc nnnnnnnnnn ttttcctttc tggaggtgaa tgacggttcc tccgtcgcct
    11701 ccctgcaggt agtggcggat gagggtattc ccggctatga acggattgga gaaatggcta
    11761 cggggtctgc cgtgagcatc cgtggcgctc tggttgccgg gcagggaagg cagcgatggg
    11821 aattgcgcgc cgcttccctg gagctggtgg gggctgttcc ggattcctat cccttgcaga
    11881 aaaaggggca tacgccggag tttctgcgtt ccatcgccca tttgcgcccc cgcaccaacc
    11941 tgtttggagc cgtgttccgc atgcgcagca ggctggcctt cgccatccac cgctttttcc
    12001 aggaaaggga tttttcctgg gtcagcactc ccattattac cgccagtgac tgtgaagggg
    12061 ccggagaaat gttccgtgtg acgactttgg atgtgggaga cgctgcttcc cgtgacgcct
    12121 cccgggattt cttcggcaag gcggcctacc tcacggtaag cggccagctg gaaggggaag
    12181 cgttcgcgtg cgccctgagc aacatttata cttttggccc cacgttccgg gcggagaact
    12241 ccaatactac gcgccatgcg gcggaattct ggatggtgga gcctgaaatg gcgttctgcg
    12301 acctgtatgg agacatggac atggcggaag agtttgtccg ttttctcgtc ggcgatgcct
    12361 tggcaaacag cgcggaagaa gtggaattcc tgacccgttt tgtagacaag gggctgtggg
    12421 aacgcctgga acatgtgaag gaaacgccgt ttgtgcgctg ttcctatacg gaagccgtgg
    12481 atattctgct caagagcggc aaaacatttg attaccccgt ttcctggggc atcaatctgc
    12541 agagcgaaca cgaacgcttc ctgacggagg aacattttaa atgccccgtt atcgtgtaca
    12601 attacccgaa ggagattaaa cccttctaca tgcgcctaaa cgaagacggg aagaccgtca
    12661 ccgccatgga tgtgctggtg cccggcatcg gcgagatcgt gggaggcagc cagcgtgagg
    12721 aacggctgga cattctggag gaaaacatgc gccgcatggg catgaatgcg gaggcgtacc
    12781 gctggtatgc ggatctgcgc cgctacggca cggtgccgca cgccggattc ggagccgggt
    12841 ttgagcgtct gttgatgttc gtgaccggag tgaccaacat ccgcgacgtg ctgccttttg
    12901 cccgtactcc gcggaattgc gagttctgac ctgccgcggg aaacggtttt tcccgtctgg
    12961 aaggatgtcg tatggttccg caggataaat gttgcggtat aaggggagag acgtgcgcaa
    13021 accgggccgg cggcaaaaga aaaccccggc tttcctttag gaataagccg gggtgaagtc
    13081 agtcgtgcgg aagaggttat tgtctctttc ttctcagcag cagtccgccc agtcccagga
    13141 ggcccagcgt ggccgttgcg ggttccggaa cggtttcgta gttaatcaac accttggtg
//
LOCUS       DAOMSU010000032        12986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_221_length_12986_cov_3.065579, whole genome shotgun sequence.
ACCESSION   DAOMSU010000032 DAOMSU010000000
VERSION     DAOMSU010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12986
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_221_length_12986_cov_3.065579"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1322)
                     /locus_tag="PK077_02625"
     CDS             complement(<1..1322)
                     /locus_tag="PK077_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HRN23226.1"
                     /translation="MFSETHSDSQRADRILASIRREEARPGRGLLKIFFGMAPGVGKT
                     CAMLEAAIQAADKGVQVIIGVAESHGREDTMRLIGRLPRLPMKKMVYRGVEMEEFDLE
                     EALRVKPQLILVDELAHTNVPGMRHRKRYQDVEDLLAAGIDVYTTLNVQHLESRSDTV
                     HDITAAPVQETVPDSVLAEADCIQLVDITPDQLRTRLREGKVYSAPQASAALDHFFKE
                     SNLTALRELALRIVAEKVDHELTEVRTISGDRSIWRSGERLMVAVGPSPFSARLVRWT
                     RRMAYALNAPWIALSVDTGVPLPPEQQQRLDANLELARRLGAEVIVMPGSDLAETLLR
                     MAFLRNVSQIVVGKPQESYLWGLVRPISLVDKLVKGSDQIDIYVVPAASGPGRNRWKS
                     WHRKTEKDGRDYWLAAGVTAAVTGAGLLVSAFTGYFAPGFLYLAGVVGL"
     gene            1814..2773
                     /locus_tag="PK077_02630"
     CDS             1814..2773
                     /locus_tag="PK077_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23227.1"
                     /translation="MLPATIASLHAETAYSTPCGYIQTTLYAKATGYLGLGVHPEALV
                     QHTLEEGNITISGNKVTITDPDVNFNDLMNENSAYVLVLHFGNEVIALPLNRDGWKKP
                     GASWTEHSIKVDDRLLSDLVSKGTPPDSYVLRKARTLDDILGEDNQFGLKSGSAMSAD
                     TVNIFNSATTKLAAYYSGNEWRRRGSTENIGNMPVFSHEPLTITRKAGSDVTAVVIGE
                     VSLKNQKLVVPTFNSLLHTRLPLSQTLAEIALQLPDDAVINIPADDKNAMVKVVNNNG
                     SWKRQDNGEDVSNIPFSGVFSIYYESSKTPSYITVSSKHQTNK"
     gene            2780..3463
                     /locus_tag="PK077_02635"
     CDS             2780..3463
                     /locus_tag="PK077_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN23228.1"
                     /translation="MFYRSLFTNAFTACALFSGEIALADSFNISNDDSSAYSLINSLG
                     EPLNAGNAILGTFSSGTWSYDVTTSRWNFQGNSYAQDQLKYSDLMSMQNNFTSALTNI
                     PEEQNGGVADGRFNLSGDYSSSLTSPVYLMVTGQDGETALFVFKDVSSDSLLNYDNID
                     ISGTETFDLWLTTKEQALTNELDYYTECILGNINLQNNTIQLLIPEPATATMSLLGLT
                     ALMVRRKRK"
     gene            complement(3653..7447)
                     /locus_tag="PK077_02640"
     CDS             complement(3653..7447)
                     /locus_tag="PK077_02640"
                     /inference="COORDINATES: protein motif:HMM:NF014926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRN23229.1"
                     /translation="MRTTRSWLLSCTALAVSSAMCGLGAPGDSAPAPAPTGLEWEQEQ
                     NLHLNKEAPTAFFASFSDLQSALKVLPENSKWRRSLNGQWKFHWAKDPQSRPVDFYKP
                     DYDVKDWKEIKVPSSWQTQGYGTPIYSNQPYPFERSWPYVMKEPSNKNYTSYKERNPV
                     GSYRRTFEVPADWDGREVYMQFDGVDSFFYLWINGQYVGFSKDSRNPARFDISPYLKK
                     GENVVAAEVYRHSDGAYLECQDMFRLSGIFRNVSIFALPKVHIRDFFAQANPVDQRDW
                     ALNIDHAKPGTVDGDWRLQVDVDVRNLFPATEKLDGCTVSMALYDAAGKLVEPVKPKD
                     APYDGVLEKPLRITGMKDFKTSLLGIYSKPRLWSAEEPNLYTLVLTLKRDGKTEEMVS
                     SRVGFRNVVIKDSVFLVNGQPVKVKGVNRHESHPETGHYVTPEQMEEEVRMMKRANIN
                     HVRCSHYPADPYFYYLCDKYGIYVQDEANIESHGYYYGKESLSHPIEWMPAHVDRIMA
                     MVERNKNHPCVIMWSLGNEAGPGQNFRSAEKMVKARDMSRPTHYERNNDIVDLGSNQY
                     PSVDWTRSMAGNKDFPKPYYISEYAHNMMNAMGNLADYWEAIESSDRIMGGAIWDWVD
                     QGLYKTLPNGEKMLCYGGDFNDHPNSGQFVFNGTILSDRTPEPGYFEVKHVYQNISTS
                     LTDDGRISIFNKNFFTDLSPYDITWTLTENGNAVAEGRLDTPPAGPREKIVVPIPDIP
                     QLKNRKPGVEYALRISYKLKKDRGWAKKGYELAFDQLQLPVQGDLPVFKAPAGKVSLS
                     TDKHTVSGKDFSVQFDAATGELAQFTVNGKPLFKTPMAVNALRAASSNEPGVMAKSMA
                     NGLRELKHELLSYEAIDNGNSVTVKQSIKVSGKQAENISGYGDTKTTITARKQPLNDT
                     NTHFINNLEWTIYADGTVVCQSVLLPRGNPLELLRLGYELQLPANMDNVAYYGRGPEE
                     NYADRKSGMPLGVYKTTAWDSFFPYGRPQDCGNHEDTRWVAVTDDKGNGLLFGSVGAP
                     FAFSALPYTTTDLILANHPVELPKTTDKTVLVLSSATRGLGSASCGPGPMGRDIIKAN
                     KPYPMSFFMRPITAKSYKGEIRVPAARLDMTMLTRTDKYTVKSVTSQEQGEADAEFAI
                     DGDPGTFWHSEYNKTVTKHPHVLVVDLGKEREFSGITYLPRQDGGSNGRVKDYSVDVS
                     TDGEKWQPAAKGSFPDSADLQEVKFQAPVKARYFRFSALSEAQGRDYAAVAELDIIPV
                     KK"
     gene            complement(7584..8645)
                     /locus_tag="PK077_02645"
     CDS             complement(7584..8645)
                     /locus_tag="PK077_02645"
                     /inference="COORDINATES: protein motif:HMM:NF021497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HRN23230.1"
                     /translation="MKCTALCMCAALLPVGFLQAGTQQIEAPQEGSVISFHLENDMFV
                     GDDDNYTNGVRFAWMSGTTSRSHTFSGMLGTVLGGTNASDSWRRFMGMSGSANLRQQW
                     GLDLTQLMYTPEQKATYPIYNQHPYVGNLTLGLTSLVKNEDRANSLELQLGTTGTNSL
                     AKGSQHFIHKLWGMEQWPGWANQLPGEITANLFFKRYYRLRGLEKRYGSGFETDALAY
                     WHADAGTVKVQAGGGMSFRFGYNLGNTSPENSIRGATSAAPPFVYNRMSVSNWGYYGY
                     IHAAVRAVAHDLYLDGTVFRSSPKYVNKYPVVGEWGYGFGLRYKRSELLFGLHYMTKE
                     YTQQESMQCVGVLQLRHTF"
     gene            8770..9585
                     /locus_tag="PK077_02650"
     CDS             8770..9585
                     /locus_tag="PK077_02650"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRN23231.1"
                     /translation="MIIDTHCHLASAQFDQSRRETYVQHALREGIDRMITLGARMDDW
                     EANTAWARQFPGAVFCALGIHPDDAHDAPADWADQLFRKAQDVPLAAIGETGLDYFHG
                     TPQGWETEQFHRLQQDLLERHFDLAERLGLNIVLHTRDRKGSASFEDALAIARNYAGR
                     VRPVFHCFIGNTAQAMRIFDELDGMVSFTGVATFKNAPVVAETASWCPENRIMLETDS
                     PYLSPEPLRGRMNEPAHLIHTARFIAAARGMNLEDLAHVTTRNAETFYNLGKV"
     gene            9655..10134
                     /locus_tag="PK077_02655"
     CDS             9655..10134
                     /locus_tag="PK077_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRN23232.1"
                     /translation="MKTPFILTCACAAALVFPLSSCSTQNSSTAGYDTAEPASGEIPP
                     WIAESSDPAYESGHYSPVQSSSSYAYNPPAKPSVTKKSSARKSTAGSKSSRSTAARKS
                     SSRKSAPKARTYTVKKGDTLGAIARRNGTSVKALKRANGLKSDLIHINQKLTIPRSK"
     gene            10164..10895
                     /locus_tag="PK077_02660"
     CDS             10164..10895
                     /locus_tag="PK077_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRN23233.1"
                     /translation="MKPLLVLAAFPVVLMGWASCSHSGTGHAALPDPRAVDKSVPEYK
                     NPFHPSTYAHFVARKDYRKTYDVYKDDTLLNRQPKKSRKIFVCLDEQRGQYLVDGLVA
                     MDFPLSSGVKAYPTKTGDYAVISKKEDHTSNLYGKMYDAEGKCINYNAESTDPVPEGG
                     RFDGSPMPYWQRLTNAGLGLHVGKVRRHPVSHGCVRLPRNVAEILYRQTSIGTPVTIQ
                     KTPLPVPEAQKIPPAQQQESEGRKQ"
     gene            11185..12564
                     /locus_tag="PK077_02665"
     CDS             11185..12564
                     /locus_tag="PK077_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRN23234.1"
                     /translation="MKRNAIDLGTLDVCTLFRKYFIPTLFGMLSISAVTAVDGILVGH
                     GIGSDGIAAINICIPLLMLFTGIGLMAGVGSSVVASIQLSKGKLEAARLNVTQALLFV
                     TIVAIVPSALMMAYPSATARFLGASEHLTPMVREYLLWFIPSLIFQMWCSVCLFMIRL
                     DGAPKLAMMCNIIAAIITVILGWLFIFPLGWGLTGAAFAATIALFASGATGLIYLWKF
                     AHKLRLYPIKWSIRSFRFSLLNIVCQCRIGSSALLTEATLATLMFVGNHTLMKYLGDN
                     GVGAFGIACYYLPFVFMVGNAIAQSAQPIISYNFGLGEKKRVADTKRIALFTSVVCGI
                     VTTAAFILFPHILVGLFVNPDSTAALIAIEGFPYFSLAFVFFIINLMLIGYYQSVENV
                     KLANLFALLRGFVFLIPCFILLPEAMGTNGAWLALCLSEMLTILTVAVLFILRKKKTL
                     LHQTGEECS"
     gene            12764..>12986
                     /locus_tag="PK077_02670"
     CDS             12764..>12986
                     /locus_tag="PK077_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23235.1"
                     /translation="MPASPERILENLFPFYRQEEFPVLLHQYKTWSESRPLEGCRIID
                     ASPVFRNTCAKYASLLAAGAELTVAVSPVM"
BASE COUNT         2984 a         3712 c         3265 g         3025 t
ORIGIN      
        1 aggcccacta ctccggccaa atacaggaaa ccgggagcaa aataaccggt aaatgcggaa
       61 accagcaggc cggcacccgt gacggccgct gtcacgcctg cggccagcca gtaatcccgg
      121 ccatctttct ccgttttccg gtgccagctc ttccaacggt tacggcccgg tcccgaagcg
      181 gcgggaacca cgtaaatatc aatctggtcg cttcccttca ccagcttatc caccagggat
      241 atgggccgca ccaggcccca caggtagctt tcctgcggtt tgcccaccac aatttggctg
      301 acatttctta aaaaagccat gcggagcagc gtttccgcca aatcggaacc gggcatcacg
      361 atcacttccg cgcccagccg ccgcgccaat tccagattgg cgtccagccg ctgctgctgt
      421 tcaggaggca agggcacgcc cgtatccacg gaaagggcta tccacggagc gttcagggca
      481 taagccatgc ggcgcgtcca gcgtaccagc cgggcggaaa acgggctggg gcctaccgcc
      541 accatcagac gttccccgct gcgccagatg gagcggtccc cggaaatggt acgcacctcc
      601 gtcagttcat ggtccacctt ctccgctacg atgcgcaggg ccagttcccg gagggccgtc
      661 aaattggact ccttgaaaaa atgatccaga gcggcggaag cctggggagc gctgtacact
      721 ttcccctccc gaaggcgcgt ccgcagctga tccggagtaa tgtccaccag ctggatgcaa
      781 tccgcctccg ccaatacgga atccggcacg gtttcctgca cgggggcggc tgtaatgtcg
      841 tgaacggtat cggaacggct ttccagatgc tgcacgttca gcgtggtgta aacgtctatt
      901 cccgcggcca gcaaatcctc cacgtcctga tagcgtttcc tgtggcgcat tcccgggacg
      961 ttggtatgcg ccagttcatc caccagaata agctggggct tcacgcgcaa tgcctcctcc
     1021 aaatcaaatt cctccatttc cactccccgg taaaccatct ttttcatggg aaggcgcggc
     1081 agcctgccaa tgagccgcat cgtatcttcc ctcccatggg attccgccac gccgataatg
     1141 acttgcacgc ccttgtccgc cgcctgaatg gccgcctcca gcatggcaca tgttttgcct
     1201 actcccggcg ccatgccaaa gaagattttc agcaagccgc ggccgggacg ggcttcctcc
     1261 ctccggatgg aggccagaat cctgtccgcc cgctggctgt ccgaatgcgt ttctgaaaac
     1321 atgaaaagag gaatcggaat gatatccggc ttgaatgttg gaatcaacct aatggaagaa
     1381 cgttcagggc gcaaataaaa caacccggaa aaaacattaa aattttatta aatcctctcc
     1441 cctgttttca ggctctcccc accccttcct ctgccaagca ttcctgaaat ctttttttca
     1501 tgattttccc cttccaggtt ccgcaccgta tctctaccac cctgcctccg tcctgttttt
     1561 cctgtgtccg gccattggaa gttactgttc cggaggcatt gaaaaaagcg gcggcagccc
     1621 ttcctgcgct tcaaatcccg gggccatccc ccggacaaac ctgaaagctt cctccatccg
     1681 gggaaaagtc tgacaaatga aaaacgggaa tgtttccccc tttcctaatt aacaaacgat
     1741 ttcctttctt cttaatgggt taatgtttat caatccggta cattgtctgc cagcctctct
     1801 ctgcgcatgc gcgttgcttc cggcaacaat cgcctccctg catgcggaaa cggcttacag
     1861 tacgccctgc ggctatatcc agacaaccct gtacgccaaa gcaacgggtt atctgggttt
     1921 aggggtgcat ccggaagcct tggtacagca cacccttgaa gaaggcaaca ttaccatttc
     1981 cggtaataaa gtcaccatca cggacccgga cgttaatttc aacgacctga tgaatgaaaa
     2041 ctccgcctat gtgctggttc ttcacttcgg caatgaagtt atagctcttc ccttaaaccg
     2101 ggatggttgg aaaaaaccgg gagccagctg gacggagcat tccattaaag tggatgaccg
     2161 ccttctttct gatctggtaa gcaagggcac tccccccgat tcatatgtcc tgcgcaaggc
     2221 gcgcactctg gatgatattc ttggagaaga caaccagttt ggcctgaaaa gcggctccgc
     2281 catgtctgca gacacggtta atatctttaa ttcggcaacc accaagcttg ccgcttatta
     2341 ttctggaaat gaatggagaa gaaggggatc tacggaaaat atcggcaata tgccggtgtt
     2401 cagccatgaa cccctgacca ttacgcgcaa agctggttcc gatgttacag ctgtggtcat
     2461 tggggaagta tccctgaaga atcagaagct ggtcgttccc acattcaatt cccttcttca
     2521 cactcgcctg cccctttccc agactttggc tgaaatagct ttgcaattgc ctgatgacgc
     2581 cgtcatcaac attcccgcag atgacaaaaa tgccatggtc aaggtcgtca acaacaacgg
     2641 ttcctggaaa cgtcaggaca atggagagga tgtttccaat atccccttca gcggagtttt
     2701 ttccatctac tacgaatctt ccaagacacc ttcctacatc acggtgtcca gcaaacatca
     2761 aaccaacaaa taaccagcca tgttctatcg ctcccttttc accaatgctt tcaccgcctg
     2821 cgctctcttt tctggagaaa ttgccttagc ggacagtttc aatatcagca atgacgattc
     2881 ctccgcatat tccctaatca attctctcgg agaaccgctg aatgcaggca atgccatttt
     2941 aggcaccttc agcagcggta cttggagtta tgatgtgacc accagccgat ggaacttcca
     3001 aggcaattcg tatgcacagg atcaactgaa atacagtgat ctgatgagca tgcagaacaa
     3061 cttcaccagc gcgttgacaa atataccgga agagcaaaat ggcggcgtcg ctgacggcag
     3121 atttaacttg tccggagatt actcttcttc tctgacatcc ccggtttatc taatggtgac
     3181 gggacaggat ggagaaacag ctctttttgt ctttaaagac gtatccagcg acagtctgct
     3241 taattacgat aatatagata tatccggcac cgagacgttc gatctgtggc ttacgaccaa
     3301 ggagcaggct ctaacaaacg agcttgacta ctacaccgaa tgcatcctgg gaaacatcaa
     3361 tctgcaaaac aataccatcc agcttttgat cccggaaccc gcgacagcca ccatgagctt
     3421 gctgggtttg acggccctga tggtacgccg taaacggaaa tagaagcttc ccttttcatc
     3481 aaacccctgt acgggtattt tccgtacagg gttttttgtt ctgccaattg ttcatcctcc
     3541 atcttcttct gcattttccc tccagaaaac ccatggagcc cggcaaataa aacgccccgc
     3601 gcactcggaa agagcggcgg ggcgcctgtt caaacggtaa aaaccgcagg agttatttct
     3661 taacgggaat gatatccagt tccgctacgg cggcgtaatc ccgcccctgc gcctcactaa
     3721 gggcggaaaa acggaaataa cgcgccttga cgggagcctg gaatttcact tcctgcaggt
     3781 cagcactatc cgggaaggag cccttggcgg caggctgcca tttctctccg tccgtgctca
     3841 cgtccacgga ataatctttc acgcggccat tgctgccgcc atcctgacgt ggaagataag
     3901 tgattccgga gaattcccgc tccttaccca ggtccacgac cagtacatgc ggatgtttgg
     3961 tcacggtttt attatattca gagtgccaga aggtgccggg atcaccgtca atggcgaatt
     4021 cggcgtccgc ttcgccctgc tcctggctgg ttacgctctt gaccgtatac ttgtccgtgc
     4081 gggtcagcat ggtcatatcc agccgggccg caggcacgcg gatttccccc ttgtaggact
     4141 tggcggtaat gggccgcata aagaaggaca tcgggtaggg cttgttggcc ttgatgatgt
     4201 ccctgcccat ggggccggga ccgcaggaag cactccccag gccgcgcgtg gcggaagaga
     4261 gaaccagcac ggtcttatcc gtcgtcttcg gcagttccac ggggtggttt gccaggatca
     4321 aatccgtggt ggtatacgga agggcggaga aagcgaacgg tgcgcccacg gaaccgaaaa
     4381 gcaggccgtt ccccttgtcg tccgtaacgg ccacccagcg ggtatcctca tggttgccgc
     4441 aatcctgcgg tctgccgtac gggaagaaag aatcccaggc tgtcgtttta tacacgccca
     4501 gaggcatgcc gctcttgcgg tccgcatagt tttcttccgg cccgcgcccg taataggcta
     4561 cgttgtccat attcgccggc aactggagtt cgtatcccag gcgcagcagt tccaggggat
     4621 tgccgcgcgg aagcagtacg gactggcaga cgacagttcc atccgcatag atggtccatt
     4681 ccaaattatt gatgaaatgg gtgttcgtat cgttcagggg ttgcttcctg gccgtgatgg
     4741 tggtcttggt atcgccgtag ccgctgatgt tttcagcctg tttgccgctc accttgatgg
     4801 attgtttgac ggtgacgcta ttgccgttat caatggcttc gtaactgagc agttcatgct
     4861 tcagttcacg gaggccgtta gccatgctct tggccatgac gcccggctca ttgctggagg
     4921 cggcgcgcag ggcgttcacc gccatgggcg ttttaaacag aggcttgccg tttaccgtga
     4981 actgggccag ttccccggtg gccgcgtcaa actgcacgga aaaatccttg ccggaaacgg
     5041 tatgcttgtc cgtgctgagg ctgactttgc ccgcaggagc cttgaacaca ggcagatctc
     5101 cctgcacggg aagctggagc tggtcaaagg ccaattcata tcccttcttg gcccaccccc
     5161 tgtccttctt cagcttgtaa cttatgcgca aggcatactc cacccccggt ttccggtttt
     5221 tcaactgggg aatgtccgga atgggaacca caatcttttc tctgggaccg gccggaggcg
     5281 tgtccagcct gccttcagcc actgcattgc cgttttccgt gagggtccag gtaatgtcgt
     5341 atggagaaag gtccgtaaag aaattcttgt tgaaaatgga aatcctgccg tcgtccgtca
     5401 aagatgtgga aatattctga tagacgtgct tgacttcaaa ataacccggt tccggcgtac
     5461 gatccgacag gatggtgccg ttgaacacaa actggccgct attgggatgg tcgttgaaat
     5521 cgccgccgta gcagagcatc ttttctccat tcggcagggt cttgtacaag ccctggtcca
     5581 cccagtccca gatggcgccg cccataatgc ggtcggaaga ctcgatggcc tcccagtaat
     5641 ccgccaggtt gcccatggcg ttcatcatgt tgtgtgcgta ctcggaaata tagtagggct
     5701 tgggaaaatc cttgttgccc gccatggaac gcgtccagtc cacggacgga tactggttgg
     5761 accccaggtc cacaatgtca ttattgcgtt cgtagtgggt ggggcgggac atatccctgg
     5821 ccttcaccat cttttccgca ctgcggaaat tctggccggg gccggcttca ttccccaggg
     5881 accacataat cacgcaggga tggttcttgt tgcgctccac catcgccatg atgcggtcca
     5941 cgtgggccgg catccactca atgggatggg aaagggactc cttgccatag taatagccgt
     6001 gggactcgat gttggcctca tcctgcacgt aaatgccgta cttgtcacag aggtaataaa
     6061 aataaggatc cgcgggataa tgggaacagc gcacgtggtt gatattggcg cgtttcatca
     6121 tccgcacttc ttcctccatc tgctccggag tcacgtaatg ccctgtttcc ggatggcttt
     6181 catggcggtt cacgcccttt accttcaccg gctggccgtt taccaggaac acgctgtcct
     6241 taatcaccac attgcggaaa cccacgcggg aggaaaccat ttcttccgtc ttcccgtcac
     6301 gcttcagcgt cagtaccagg gtgtacaggt tgggctcctc cgcagaccat agtctgggtt
     6361 tggaataaat gcccagcagg gaagttttaa aatccttcat gccggtaatg cgcaagggct
     6421 tttccaacac gccgtcatat ggcgcatcct tgggcttgac aggttctacc agttttccgg
     6481 cagcgtcata aagggccatg gaaaccgtgc agccgtccag cttttccgtt gccggaaaca
     6541 gattgcgaac atccacatcc acctgaaggc gccaatcgcc gtccacggtc cccggtttgg
     6601 catggtcaat attcaaagcc caatccctct ggtccaccgg attggcctgt gcgaaaaagt
     6661 cgcggatgtg aactttcggc agcgcaaaga tggaaacatt gcggaaaatg ccggacaggc
     6721 ggaacatatc ctggcattcc agatatgctc cgtcggaatg gcggtacacc tctgcggcca
     6781 ccacattctc ccccttctta aggtaggggc tgatgtcaaa acgggccgga ttccgggaat
     6841 ctttggaaaa acctacatac tgcccgttga tccagaggta gaagaaggaa tccactccgt
     6901 caaactgcat gtacacttcc ctgccgtccc agtccgcagg cacttcaaaa gtgcggcggt
     6961 aggaacctac ggggttccgt tccttgtagg acgtataatt cttgttggaa ggctccttca
     7021 ttacataagg ccaggagcgt tcaaagggat acggctgatt ggaataaatg ggggtgccgt
     7081 aaccctgagt ctgccaggag gaaggcacct taatctcctt ccagtccttt acgtcgtaat
     7141 ccggcttgta aaaatcgacc gggcggctct ggggatcctt cgcccagtgg aacttccact
     7201 gaccgttcag ggacctgcgc catttactgt tttcaggcag cactttcaag gcggactgca
     7261 aatcgctgaa agacgcgaaa aaggcggttg gagcttcctt attcaaatgc aggttttgtt
     7321 cctgttccca ttccagccct gtgggggctg gcgcgggggc ggaatcgccc ggtgctccca
     7381 ggccgcacat ggcggaactt accgccagcg cggtacagga taaaagccag gatctggtcg
     7441 ttctcatgaa atcaatatgt gtcggagttg atatgcacaa acggattgtc ctgttacata
     7501 cgaatccgca attctaattc taacagcaaa cttgcccttt gcaagctgct gttgcgttta
     7561 tgaacggcct gcaaacgcag ccgttaaaaa gtatgccgga gctggagaac gcccacacac
     7621 tgcatggatt cctgctgggt gtactccttg gtcatgtaat gcaggccgaa aagcaattcc
     7681 gagcgcttgt accggaggcc gaagccataa ccccattctc ccactacggg atacttgttc
     7741 acatacttgg gggaggaacg aaacaccgta ccatccagat acaggtcatg agccacggct
     7801 cgcacggcag catgaatata accgtaatac ccccaattgg aaacggacat cctgttataa
     7861 acgaagggag gcgctgcgct ggtcgcgccg cgaatgctgt tctccggaga agtattgccc
     7921 agattatagc cgaagcggaa ggacatgccg ccccccgcct gcacctttac tgtgccggcg
     7981 tccgcatgcc agtaagccag ggcatccgtt tcaaaaccgg agccgtagcg cttctccagt
     8041 ccgcgcaggc ggtaataccg cttgaaaaac aaattggcgg ttatctctcc ggggagctgg
     8101 ttggcccagc cgggccattg ctccataccc cacagcttat ggatgaaatg ctgggagccc
     8161 ttggcgaggg aattcgtgcc cgtggtgccg agctgcagct ccagggaatt ggcccggtct
     8221 tcattcttaa ccagggaggt cagccccagc gtcaggttgc ccacataggg gtgctggttg
     8281 tagatgggat aggtggcctt ctgctccggg gtgtacatga gctgggtcag gtccaggccc
     8341 cactgctggc gcaggttggc ggaaccgctc atgcccatga accgccgcca ggaatccgag
     8401 gcgttcgtgc cgcccagcac tgttcccagc atgccggaaa acgtatggct tcgggacgtg
     8461 gtgccggaca tccacgcaaa gcggacgccg ttggtataat tatcatcatc ccccacgaac
     8521 atatcgtttt ccagatggaa actgataacg gagccctcct gcggcgcttc tatctgctgg
     8581 gttcctgcct gcaggaagcc caccggcagc agggcggcgc acatgcacaa agccgtacat
     8641 ttcatgcggg gcagtttgtg cgtttcactt ccgcgtggca aggaaaaatc aggcatgctc
     8701 tgcgtttgca cttttcaaag ccggcaaata tccacaaact tcatccgttc cgccacagtc
     8761 ccttacacca tgattattga cacgcactgc catctggctt ccgctcaatt cgaccagtcc
     8821 cgcagggaaa cttacgtcca gcatgccctc cgggaaggca ttgaccgcat gattacgctg
     8881 ggggcgcgga tggatgactg ggaggcaaac acggcgtggg cgcgccagtt tcccggcgcg
     8941 gttttttgtg cgctgggcat ccatccggac gatgcccatg acgccccggc agactgggcg
     9001 gaccagcttt tccggaaggc gcaggacgtt cccctggccg ccatcgggga aacgggcctg
     9061 gattacttcc acgggacgcc ccaaggctgg gaaacggaac aattccaccg ccttcagcag
     9121 gaccttctgg aacggcactt tgacctggcg gagcgcctgg gccttaatat cgtcctgcat
     9181 acgcgggacc ggaaaggttc cgcgagcttt gaggacgcct tggctattgc ccggaactac
     9241 gcgggccgcg tgcgtcccgt atttcactgc ttcatcggaa ataccgctca ggccatgcgg
     9301 atctttgacg aactggacgg catggtctcc ttcaccgggg tggctacttt taaaaacgcc
     9361 cccgtagtgg cggaaaccgc aagctggtgc ccggaaaacc gtatcatgct ggaaacggac
     9421 tccccctatc tcagcccgga acccctccgg ggccgcatga acgaacccgc ccatctcatt
     9481 catacagccc gtttcatcgc cgctgccaga ggcatgaacc tggaagatct ggcccatgtg
     9541 acgacccgaa atgcggaaac cttttataat ctgggcaagg tctgaaaaaa aatcccgaca
     9601 atccgcgcta ccgccttgaa tccgaaatca taaattagta tgaacagggc acacatgaaa
     9661 acgccattca ttttaacctg cgcctgcgct gccgcgctgg tattccccct ctcttcctgc
     9721 tccacccaga attcctccac ggccggttac gacactgcgg aaccggccag cggcgaaatc
     9781 ccgccgtgga tcgcggaaag ctctgatccc gcttacgaat ccggccatta cagcccggtt
     9841 cagagttctt ccagctacgc ttacaatccc cccgccaagc cttccgtcac taaaaaatca
     9901 tccgcgcgga agagcacggc cggcagcaaa tcctcccgca gcacggctgc cagaaagagc
     9961 tcctcccgta aatccgctcc caaggcccgc acctacaccg tgaagaaagg cgatacgctg
    10021 ggagccatcg cccgcaggaa cggcacctct gtcaaggccc ttaaaagggc taacggcctt
    10081 aagtccgatc tgattcacat caaccagaag ttgacgattc cgcgttccaa gtaatccggg
    10141 ggatcctccc ggttccacca cttatgaagc ctctccttgt tctggccgcc ttccccgtgg
    10201 tgctcatggg gtgggcgtcc tgttcccact ccggcacggg ccatgcggcc ctgccggatc
    10261 cgcgtgccgt ggacaaatcc gttccggaat ataaaaaccc gtttcatcct tccacatacg
    10321 ctcattttgt agcccgcaag gactaccgga aaacgtacga cgtctataag gacgatacct
    10381 tgctgaaccg ccagccgaaa aaatcccgaa aaatcttcgt ctgcctggac gaacaacggg
    10441 gccagtatct ggtggacggc ttggtcgcca tggacttccc cctctcctcc ggcgtaaaag
    10501 cctatccgac caaaacgggg gactacgccg ttatctccaa aaaggaagac catacttcca
    10561 acctctacgg caaaatgtat gatgccgaag gcaaatgcat caactacaat gccgaatcca
    10621 cggatcccgt accggaggga ggtcgttttg acggctctcc catgccttat tggcagcgtc
    10681 ttaccaacgc ggggctgggc ctgcatgtag ggaaagttcg gcggcatcct gtttcccatg
    10741 gctgcgtccg tctgccgcga aatgttgcgg aaattctcta caggcaaacc agcataggaa
    10801 cgcccgttac catccagaaa acgccgctcc ccgtgccgga agcgcagaaa atccctcccg
    10861 cacaacaaca ggaatccgaa ggccgtaaac aatagcctac cgggaaaacg ccccctttaa
    10921 aacacaaact attttaaaaa gactgcccgg aaagcagtct tttttctatc ccattttttc
    10981 caaggcatta acatgacgta taaaaaaatt atatttccta aattattgca ctatttttac
    11041 ggccacacaa cgaaaagaag cgttttgtaa tcctcatcgg agggcaaata aaaatgccgg
    11101 ataagatgac tgggtaaaac caatcacctt atccggcatt ttttgtttcc cggcaaaaaa
    11161 tagccataag aataacagac acatatgaaa agaaatgcaa ttgatttagg cacactggac
    11221 gtatgcacat tattcagaaa gtattttatc ccgaccttat tcgggatgct tagcatatcg
    11281 gcagtaacgg cagtagacgg aatactcgtc ggccacggaa tcggaagcga tggcattgcc
    11341 gctatcaata tatgcattcc cctgctgatg ctgtttaccg ggatagggct catggcaggc
    11401 gtaggctcat ccgtagtcgc ttccattcag ctatcaaaag gaaaactgga ggccgcacgc
    11461 cttaatgtca cccaggcctt gttgttcgtc accattgtcg ccatcgtgcc atctgccttg
    11521 atgatggcat atccctcagc tacagcccga tttttaggcg cttccgaaca tcttaccccc
    11581 atggttaggg aatacctgtt gtggttcatc ccctcgttga tattccagat gtggtgctct
    11641 gtctgcctat tcatgattcg tttggatggc gctccgaagt tggctatgat gtgcaatatc
    11701 attgctgcaa tcatcaccgt aatactcggt tggctgttta tctttccatt gggctgggga
    11761 ttgacgggag ctgctttcgc cgcaaccata gcactgtttg cgagcggcgc cacgggcctt
    11821 atttatttat ggaaatttgc acataaactg cggctttatc ctattaaatg gagcatcaga
    11881 agttttcggt tctccctcct taatattgtc tgccaatgtc gcatcggttc ttccgctttg
    11941 ctgactgaag cgacactggc cacgctcatg ttcgtcggga accacacatt gatgaaatat
    12001 ctgggagata atggagtagg agcgttcggc attgcctgct attaccttcc gttcgtattc
    12061 atggtaggca atgccatagc gcaatcagcg caacccatca tcagctataa cttcgggctt
    12121 ggagagaaaa aacgcgtagc cgatacaaaa cggatcgcat tatttacctc cgttgtttgc
    12181 ggcatagtca cgacggcagc atttattttg ttcccgcata ttctggtagg cttgttcgtc
    12241 aatccggaca gcactgcggc gctcatagcc attgagggat ttccatattt ctccctggca
    12301 tttgttttct tcatcatcaa cctgatgctc atcggttatt accaaagcgt ggaaaatgta
    12361 aaactcgcta atttgtttgc cctgttgcga ggatttgttt ttcttattcc atgctttatc
    12421 ctgttgcctg aagcaatggg aacgaacggt gcgtggctag cactttgtct gtcggaaatg
    12481 ttgaccatat taacagttgc tgtcctattt attctacgca aaaagaaaac acttctgcat
    12541 caaacaggtg aagaatgcag ttaaaaagat ttttctcagg catgccatga cacgcgcata
    12601 aaatacttgc aagcgaatac agatgactta gcactttgtc ttccctgaac tggctacagc
    12661 tttaaaaatt attaaacaaa agatatttct ttaacaaagt ttttctggga aaacgacagg
    12721 atagggatta ccctctgctg cgagaaaccc taagattttt gttatgcctg catctccgga
    12781 gcgcattctg gaaaatttgt tcccctttta ccggcaggaa gaatttcccg ttctcctgca
    12841 ccagtataaa acgtggtcgg aaagtcgccc tctggaagga tgccggatta ttgacgcttc
    12901 tcccgtgttc cggaatacct gcgccaaata cgcctctctt ctggccgcag gagcggagct
    12961 gacagtagcg gtctccccgg taatgc
//
LOCUS       DAOMSU010000033        12863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_229_length_12863_cov_2.843770, whole genome shotgun sequence.
ACCESSION   DAOMSU010000033 DAOMSU010000000
VERSION     DAOMSU010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12863
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_229_length_12863_cov_2.843770"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="PK077_02675"
     CDS             <1..240
                     /locus_tag="PK077_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HRN23236.1"
                     /translation="PAPAPAAAGAGDVPSPLAGKVVSLDVAAGASVKAGDQILTLEAM
                     KMNTIIYAPASGTLTAFCVNPGDTVQEGQALAKIG"
     gene            319..1983
                     /locus_tag="PK077_02680"
     CDS             319..1983
                     /locus_tag="PK077_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRN23237.1"
                     /translation="MSLFDSLITFLQGMGIFSLSWQMVGMWGIAILLLYLGVAKQYEP
                     LLMVPIAFGALIANIPDNGMLITQLNQQVISSNEQGEVTSTSLNNVGYLRVHVAPLQQ
                     TPAKVPANLTTPEARAQYLETMQQPMQVYPGSQLTVSKIKSVRESQEKAKADAARLGD
                     DSLTVDPNLKDFQNVTEDNGNEPVFLLKNGEGTTVVRQQGVNYFDTSGNRVPVDLKTQ
                     KLEPLVVSAAGKYVAVGQHTQELLVTSIHGGLYDWIGLGIKAEIFPPIIFLGVGALTD
                     FGPLLAAPRTLLLGAAAQVGVAATFFMALFMGFNPNEAASIGIIGGADGPTSIFLTMK
                     LAPHLLGAVAVAAYTYMSLVPLIQPPIMALLTTKKERVIRMKSLRTVSKSEKLFFAVL
                     VTIVTILLIPDASPLIGMLMLGNFLRECKVTERLVQASQNEIINIVTIFLGTSVGLTM
                     QGDRFLQAETLLIILLGIVAFGVATAGGVIAAKLMNLIWRKNPVNPLIGSAGVSAVPM
                     AARVSHNVGQKYDPSNYLLMHAMGPNVAGVIGTAVIAGYYIATLAK"
     gene            2021..2800
                     /locus_tag="PK077_02685"
     CDS             2021..2800
                     /locus_tag="PK077_02685"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRN23238.1"
                     /translation="MNSAASFSTPPEEEGPCGDWTYHEWAEAFSTNDLARSLPPGHIA
                     VCRVQTGGRGRFNRKWIGEEGGLWASFTVPLSAPADTAAGVHWGHLPLVAGLALFNML
                     RGMGLDSVRLRWPNDLLAGKSKLAGILVERPAENMAVIGIGINVFNDVSSIAGEIKDP
                     PARLADLVRDCPSLHAVMASLGSHLNQVLSVFSHGGVSALQEGLSRAWAGERPVSILT
                     DDETVRGIFTGIDDQGNPVLSLPAGTVRTIPAHLINRLLEE"
     gene            2861..4357
                     /locus_tag="PK077_02690"
     CDS             2861..4357
                     /locus_tag="PK077_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HRN23239.1"
                     /translation="MSYKVLTPEEAASLIKHGHHIGLSGFTPAGTAKAVTAALAKIAE
                     AEHEKGNPFQVSVFTGASTGDSCDGILSRAKAIRYRAPYTTNVDFRKAVNNGEIAYND
                     IHLSQMAQEVRYGFMGNVDIAIIEACEVTADGKIYLTAAGGIAPTICRLADKIIVELN
                     AAHSKSAMGLHDVYEPLDPPYRREISIFKPSDRIGKPYIQVDPKKIVGVVETNWPDEA
                     RSFAAADPLTDKIGQNVADFLAADMKRGIIPSTFLPLQSGVGNIANAVLGALGRDKTI
                     PAFEMYTEVIQNSVIGLIREGRVKFGSACSLTVTNDCLEGIYRDMDFFRDKLVLRPSE
                     ISNSPEVIRRLGVISINTAIEADIYGNVNSTHIGGTKMMNGIGGSGDFTRNAYISIFT
                     CPSVAKDGKISAIVPMVSHHDHTEHDVNIIITEQGVADLRGKSPKERAQTIIENCVHP
                     DYKNILWDYLKLSDGKAQTPQSIRAALGMHAELARSGDMKNVDWAQYK"
     gene            4477..5178
                     /locus_tag="PK077_02695"
     CDS             4477..5178
                     /locus_tag="PK077_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23240.1"
                     /translation="MNWMIYPAARAFIKVCAVLFGAGATAAALIHLFMPFFLRSPRTA
                     APFLLLGTGAELLSILSLSFLMFWGARVLLASRGSRITRNLSMLAFLPALYALLIPFA
                     GMPVPSQYTEMDRVWEAFLLLASVISFFSFPYTAGYGAAGRRLFLAWGIAAAGCGSQS
                     ALFFLALLLRQFLSTAVPSLAAPISWLAVLLSCGLEIMLAALSVILARRLFKEVMSIA
                     SMPEPIDPSMPGNLP"
     gene            5237..5485
                     /locus_tag="PK077_02700"
     CDS             5237..5485
                     /locus_tag="PK077_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23241.1"
                     /translation="MNTRTAHGSLLPTRLPHPGMKGFYGFEQTTAGNFRKYGHRMESI
                     AGKQTAPTENRGICRVAPSISLADPRQPYFTNSSKYRP"
     gene            complement(5452..6315)
                     /gene="rfbA"
                     /locus_tag="PK077_02705"
     CDS             complement(5452..6315)
                     /gene="rfbA"
                     /locus_tag="PK077_02705"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRN23242.1"
                     /translation="MKGIVLAGGSGTRLHPVTLSVSKQLLPVYDKPMVYYPISVLMLA
                     GIREILVISTPRDLPLYRDLLGDGSRFGVSFEYREQPRPEGLAQAFIIGEEFIGKDSV
                     ALVLGDNIFYGRGLTEKLRRAAELTSGATIFGYQVHDPQRFGVVEFNEEGKAVSIEEK
                     PLRPKSDIAVTGLYFYDNRVVEIAKRIPKSSRGELEITSVNQEYLKQGLLRVEKLARG
                     YTWLDTGTHDTLLEASMFVRTLEHHQGFKVACLEEIAFNNGWISPETLRSQAGAMNNN
                     GYGRYLDELVK"
     gene            complement(6325..7377)
                     /gene="rfbB"
                     /locus_tag="PK077_02710"
     CDS             complement(6325..7377)
                     /gene="rfbB"
                     /locus_tag="PK077_02710"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRN23243.1"
                     /translation="MKVIVTGGAGFIGSNLVRLLLERGHEVLNIDALTYAGNIHSLDG
                     SADSPAYRFLHADVRDASLMRRTLREYAPDWVMHLAAESHVDRSIDDPGNFMTTNVMG
                     TFSMLQAALEYYRSLEGEKKERFRFHHISTDEVYGSLGKEGLFTESTPYRPHSPYSAS
                     KASSDHLVRAWHDTYGLPALITNCSNNYGPYQFPEKLIPVVILKALGGESIPVYGKGE
                     NVRDWLYVGDHCEALLTVVSCGKPGETYNIGGNNEKTNLELVELLCSHLDELRPRPDG
                     RSYREQISFVQDRPGHDLRYAIDASKIRRELGWIPRQDHSSGFRKTIQWYLDHEEWWR
                     GILSGEYRLERLGTNG"
     gene            complement(7509..8453)
                     /locus_tag="PK077_02715"
     CDS             complement(7509..8453)
                     /locus_tag="PK077_02715"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRN23244.1"
                     /translation="MTSGGDAAGMNPAIKAAVDHARQLGMNPYVVYDGLEGLIDGKIK
                     EATRELTNDMIYRGGTLLRSSRSKRFFDYEFRKQAYEHLKKRNIDKLIIMGGDGSFRA
                     LNDFFNDFEVPFAGIPATIDNDIAGTDYCLGVDTAQNVILDCIDSVRDTARSHHRAFV
                     IETMGRDCGYLAMTTALCSAADICIVPEIPYDLESIYKRLQPVINEEGSCIIAVVAEG
                     TRVAQYLTRWLTERAGMDTRLTVLGHVQRGGSPTARDRLMGTRFATRAVEALNNGDIN
                     QIMVYHDGQYGYASLDSVAGQQYSVNQDIINLCRELSC"
     gene            8582..9313
                     /locus_tag="PK077_02720"
     CDS             8582..9313
                     /locus_tag="PK077_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-strand selective monofunctional
                     uracil-DNA glycosylase"
                     /protein_id="HRN23245.1"
                     /translation="MNDTAAAILKATAALRDGTEKLRFEAPVHVTYNPLTYAWGPHEQ
                     YVRTYGNGEKSHLFLGMNPGPFGMAQTGVPFGEVDAVVNWLHIRGEVGRPEHTHPKRP
                     VEGFSCPRSEVSGRRLWGLFAEAFGTAENFFRHNYVANYCPLIWMGATGANITPDKLP
                     TEQRAAVDAVCMEHLLSLITILNPHTLVGVGAYATQKLRDAASRLPGKSFTIGTLLHP
                     SPASPIANKLWPERPIQQLKELGIL"
     gene            9678..11144
                     /gene="gatB"
                     /locus_tag="PK077_02725"
     CDS             9678..11144
                     /gene="gatB"
                     /locus_tag="PK077_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRN23246.1"
                     /translation="MAISDYIVTIGLEVHCQIKTKSKMFCSCETGFGFEPNTRVCPTC
                     LGLPGALPVLNEFAIERTLLTGLMLGCSSPEISQWDRKNYFYPDMAKNYQTSQLDLPL
                     CLGGEVPLYPWSYPNDVIKAGVPPFRTVKLTRIHLEEDAAKITHHANYSLIDYNRAGS
                     ALMEIVSEPDIDTPEEAYAYLKSLQQILNYGDISDADMEKGQMRCDVNISLRPHGQEE
                     LGAKVEMKNINSMSAVRRALHYEIRRQAEELDMGIPQIQSTRRWDDERGESVVMRTKE
                     DAHDYRYFPCPDLVPVRTAPLVEKVRGQIPELPQERQKRFMEEYGLSEYDANVLVGDL
                     ELAHFFEEAARGTASGKKIANWVINNISAVLNEKGMRPSECPVKPGAIRELIAIIDDG
                     TVSNNQAKDVFAKMWDEPALAAAQAAKLLGFEKADSSFLDGIVREVISANPDKVAEIQ
                     GGNEKLLNWLTGQVMKAAKGKVNPKIVTEALKKALSME"
     gene            complement(11199..>12863)
                     /locus_tag="PK077_02730"
     CDS             complement(11199..>12863)
                     /locus_tag="PK077_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HRN23247.1"
                     /translation="IVGDSLNPVTDALRRRKTIEWIHVRHEEVAAFAASAEAQLSGNL
                     AVCCGSSGPGNLHLINGLFDAHRNHAPVLAIASHIPQSQVGMEYFQATHPEQLFKECS
                     AYAELVSEASQAPCILMSAIQHAWARRDVGVVVLPGDIADAPAEMKNLVHPPSYTREN
                     VVPEHESVSLLARMLDDHRRITFFCGAGCAGAEDKVVRLAHRLKAPIAYTWRGKDYFE
                     HDNPLGIGMTGLLGWGDAYKAMHESDMLVLWGTDFPYFNFIPTKPEIVQIDRRGEVLG
                     RRCRLDLGICGDVSVTAEALLNMVQEKTDSGHLDAALKRHARDVKEMNAYMEGNDKES
                     PIRPEQLTTALNRHAASDAVFTVDTGTPCIWSARFLCATLGRRTLASFNHGSMANAMP
                     MAIGAQMEYPGRQVIALCGDGGLSMLLGDLITIVQYRLPVKIVVYNNDCLDFIQLEMQ
                     AAGLIPWQINLNNPSFAAVADAVGIKGFLLDKSSQVDQMVNMFLNYPGAALLDAHVDR
                     DALALPPYISMGQAADFSLAMMKQTLAGEVKQVWNTLAGNRKLFKP"
BASE COUNT         2763 a         4022 c         3467 g         2611 t
ORIGIN      
        1 cctgctccgg ctccggccgc cgccggcgcc ggagacgtgc ccagcccgct tgccggcaag
       61 gttgtttccc tggacgtggc cgctggcgcc tccgtgaagg ccggcgacca gattctgacg
      121 ctggaagcca tgaagatgaa taccatcatc tacgctcccg cctccggcac gctcaccgct
      181 ttctgcgtaa acccgggtga tacggttcag gaaggacagg ctctggccaa aattggttaa
      241 tcccggcggc aaccgcccgg aacctccaac agttcctggc tccgactcag ataacaaacc
      301 gctacaataa cattccgtat gagtcttttt gattcattaa tcaccttttt acaggggatg
      361 ggcatcttct ccctatcatg gcaaatggta gggatgtggg gcattgccat cctgctcctg
      421 tatctgggtg tagccaagca atacgaacct ctcctgatgg ttcccattgc tttcggcgcc
      481 ctcatcgcca acattccgga caacggaatg ctcatcaccc agctgaacca gcaggtcatc
      541 tcctccaatg aacaggggga agtaacctcc acgtccctga acaacgtggg ctacctgcgc
      601 gttcatgtgg ctcctctgca gcagacgccc gccaaggtgc cggccaatct gaccacgccg
      661 gaagcccgcg cccagtatct ggaaaccatg cagcagccca tgcaggttta tcccggcagc
      721 cagctgaccg tttccaaaat caagtccgtg cgggaatccc aggaaaaagc caaagctgac
      781 gccgcccgcc tgggagacga cagtctgacg gtggatccca acctgaagga cttccagaac
      841 gtgacggagg acaacggcaa tgagccggtc ttcctgctca agaacggaga aggaaccacc
      901 gtcgtccgcc agcagggcgt caattacttt gacaccagcg gcaaccgtgt tcccgtggac
      961 ctgaaaacgc agaagctgga acccctggtg gtttccgccg caggcaaata cgtggccgta
     1021 ggccaacata cccaggaact gctggtcacc tccatccacg ggggcctgta tgactggatc
     1081 gggctgggca tcaaggctga aattttcccg cccatcatct tcctgggcgt aggagccctg
     1141 acggacttcg gcccgctgct cgccgcaccg cgcaccctgc ttctgggcgc ggcggcccag
     1201 gtgggcgtag ccgccacgtt cttcatggcc ctgttcatgg gcttcaaccc gaatgaagcc
     1261 gcctccatcg gcatcatcgg cggagctgac ggccccactt ccatcttcct gacgatgaag
     1321 ctggctccgc acctgctggg cgccgtggcc gtggccgcat acacgtacat gtccctggtt
     1381 ccgctgatac agccgcccat catggcgttg ctgacgacca agaaggaacg cgtcatccgc
     1441 atgaaatccc tgcggacggt cagcaagagc gaaaaactgt tcttcgccgt gctggtaacc
     1501 attgtcacca ttctgctgat tccggacgca tccccgctca tcggcatgct gatgctcggc
     1561 aacttcctgc gcgaatgcaa ggtgacggaa cgcctggttc aggcctccca gaatgaaatc
     1621 atcaacatcg tcaccatctt cctgggaacc tccgtgggtc tcacgatgca gggagaccgc
     1681 ttcctgcagg cggaaacgct gctgatcatt ctgctgggca ttgtcgcctt cggcgtagcc
     1741 actgcgggcg gcgtcatcgc cgccaagctc atgaacctca tctggcgcaa gaatccggtc
     1801 aaccccctca tcgggtccgc aggcgtctcc gctgtcccga tggcagcccg cgtctcccac
     1861 aatgtgggcc agaagtacga cccctccaac tatctgctca tgcacgccat gggcccgaac
     1921 gttgcaggcg tgatcggcac cgcagtcatc gcgggctact acatcgccac tctggcgaaa
     1981 taaccagaat tgaaaattac tcccggcctc ggcccgtccc atgaattccg cagcctcatt
     2041 ttccacccct ccggaggagg aaggaccatg cggcgactgg acttatcatg aatgggccga
     2101 ggctttttcc accaatgacc tggcccgctc cctgcccccg ggacatattg ccgtctgccg
     2161 cgttcagaca ggaggccgcg gcaggttcaa ccgcaaatgg ataggggagg aaggcggcct
     2221 gtgggcatcc ttcaccgttc ccctgtccgc tcctgcggat acagcggccg gcgtgcactg
     2281 gggccatttg cccctggttg cggggctggc cctgttcaac atgctccgcg gcatggggct
     2341 ggattccgtg cgcctgcgct ggccgaacga cctgctggcg ggcaaatcca aactggcggg
     2401 aatactggtg gaacgcccgg cggagaacat ggccgtcatc ggcatcggca tcaatgtttt
     2461 caatgatgta tcctccatcg ccggggaaat caaggaccct ccggcaaggc tggcggatct
     2521 tgttcgggac tgcccttccc tgcacgccgt catggcttct ctgggcagtc atttgaacca
     2581 ggttctttcc gtcttttccc acggcggcgt ctctgccctt caggagggtc tgtcccgggc
     2641 atgggccgga gaaaggccgg tttccattct tacggatgac gaaaccgtcc ggggcatttt
     2701 tacagggatt gacgatcagg gcaatcctgt gctatccctt ccggccggca ccgtccgcac
     2761 cattccggcg cacctgatca acaggctcct cgaagaatga gcctaccgct ccgttgttac
     2821 acggggtttc tttctttttc atccaacaaa aatcactgac atgagttaca aggtactgac
     2881 accagaagaa gcagcaagcc tgatcaagca tggccaccat attgggctga gcggattcac
     2941 gcccgccgga acggccaagg ccgttacggc tgcgctggca aaaatcgcag aagcggaaca
     3001 tgagaagggg aaccctttcc aggtgagcgt cttcaccggt gcttccaccg gcgattcctg
     3061 cgacgggatc ctttcccggg ccaaggcaat ccgctaccgc gctccctaca ccaccaacgt
     3121 tgatttccgc aaggcggtga acaacgggga aatcgcctat aatgacatcc atttgtccca
     3181 gatggcccag gaagtgcgct acgggttcat gggcaacgtg gacatcgcca ttattgaagc
     3241 ctgtgaagtc acggccgacg gcaaaattta cctgacggcc gcgggcggga tcgctcccac
     3301 catctgccgc ctggcagaca aaattatcgt ggaattaaac gccgcccaca gcaagagcgc
     3361 catgggcctg cacgacgttt acgaacccct ggatcctccc taccgccgcg aaatttccat
     3421 cttcaaacct agcgaccgca tcggcaagcc gtacatccag gttgacccga aaaagattgt
     3481 gggcgttgtg gaaaccaact ggccggatga agcccgttcc ttcgccgcag cggatcccct
     3541 gaccgataaa atcgggcaga acgtggccga tttcctggct gccgacatga aacggggcat
     3601 cattccctcc acgttcctgc ctctccagtc cggcgtgggc aacatcgcaa acgcggtgct
     3661 gggcgccctg ggccgcgaca agacgattcc cgcgttcgag atgtacacgg aagtcatcca
     3721 gaattccgtc atcggcctga ttcgggaagg ccgcgtgaaa ttcggcagcg cctgctccct
     3781 gaccgtgacg aacgactgcc tggaaggcat ttacagggat atggactttt tccgcgacaa
     3841 actggtgctc cgtccctcgg aaatatccaa cagcccggaa gttatccgcc gtctcggcgt
     3901 catctccatc aacaccgcca ttgaagccga catttacggg aacgtcaact ccacgcacat
     3961 tggcggcacg aagatgatga acggcatcgg cggatccggc gacttcacgc gcaacgccta
     4021 catctccatt ttcacctgcc cgtccgttgc caaggacggc aagatcagcg ccatcgtccc
     4081 catggtttcc caccatgacc atacggaaca tgacgtcaat atcattatta ccgagcaggg
     4141 ggtcgcggac ctgcgcggca agagcccgaa agaacgggcc cagaccatta ttgaaaactg
     4201 cgtccacccg gattacaaaa acatcctctg ggattacctg aagctgtccg acggcaaagc
     4261 ccagacaccg caatccatca gggccgcttt gggcatgcac gccgaactgg ccagaagcgg
     4321 agacatgaag aatgtcgact gggcccagta taagtaattc cgcatgccgt cattcgcatt
     4381 ggcatccatg aattcacgtt aacccctcag gaccgcttcc gcaaagggag cggtcctttt
     4441 cctttttccc atcccctcta ctctcttctc ttcaccatga actggatgat ttacccggca
     4501 gccagggctt tcatcaaagt ctgcgccgtt cttttcggcg cgggcgcaac agcagcagcc
     4561 ctcatccatc tgttcatgcc ttttttcctc cgctctcccc ggacagccgc gcccttcctg
     4621 cttttgggaa caggggcgga attgctctcc atcctttccc tgtccttcct catgttctgg
     4681 ggcgccagag tcctgctggc ctcccgcgga agcagaataa caagaaatct gagcatgctg
     4741 gccttcctgc ccgccctgta cgccctcctc attccgtttg cgggcatgcc cgtcccatcg
     4801 caatatacgg aaatggacag ggtttgggag gccttcctcc ttctggcgtc cgtcatttct
     4861 ttcttttcct tcccttatac ggcgggatac ggcgccgcag gcaggagatt attcctggcc
     4921 tggggcatcg cggcggcagg atgcggttcg cagagcgccc tgttttttct tgccctcctg
     4981 ttgcgtcagt tcctgtccac agcagttccc tccctggcag cgccaatttc atggctggcc
     5041 gttctcctgt cctgcggcct agaaatcatg ctggcggccc tttccgtcat tctggcgcgg
     5101 cgcctgttta aagaggtcat gtccatagcc tccatgccgg aaccgattga tccctccatg
     5161 ccgggcaacc ttccctgact tcccccctgc cttcatgttt gcagccagtt ggaagggatt
     5221 acccccagtc cgcggcatga atacgcgaac cgctcacggc agccttcttc caacccggct
     5281 gccgcatccc ggcatgaaag gcttttacgg ttttgagcaa acaacggccg gaaatttccg
     5341 caagtacggc caccgcatgg aaagcatcgc cgggaagcaa accgccccca cggaaaaccg
     5401 cgggatttgc agggtcgccc cttccatttc ccttgcagac cccagacagc cctatttcac
     5461 caattcatcc aaataccggc cataaccgtt attattcatg gctccggcct gggaacgcag
     5521 cgtttccggg gaaatccagc cgttattaaa ggcaatctcc tccaggcacg ccaccttgaa
     5581 cccctggtgg tgctccaacg tgcgaacgaa catggaagcc tccagaaggg tatcgtgcgt
     5641 gcccgtatcc agccacgtat agccgcgcgc cagcttttcc acgcgcaaca ggccctgctt
     5701 caggtattcc tgattcacgg aggtaatttc caactcgccc cgggaggact tgggaatcct
     5761 tttggctatc tccaccaccc ggttgtcgta aaaatagagg cccgtcaccg caatatccga
     5821 tttgggccgg agcggttttt cctcaatgga aaccgccttc ccctcctcat tgaattccac
     5881 taccccgaaa cgctgcgggt catgcacctg gtaaccgaaa atggtcgctc cgctcgtcag
     5941 ttcggcggcc cgcctcagct tctccgtcag gccgcgcccg taaaaaatat tgtcccccag
     6001 caccagggct acggaatcct ttcctatgaa ctcctctcca ataataaagg cctgagccaa
     6061 tccctccggc ctgggttgtt ccctgtattc aaaggaaacg ccgaacctgc tgccgtcccc
     6121 cagcaaatcc cggtacaggg gcagatcccg gggcgtggaa ataacaagga tttcccgtat
     6181 tccggccagc atcagcacgg agatgggata ataaaccatt ggcttatcat aaacgggcag
     6241 caattgcttg ctgacggaaa gcgtaacggg atgcagccgc gtgccgcttc ccccggccag
     6301 aacaataccc ttcatatcaa attcctagcc attggttcca agacgttcca ggcggtattc
     6361 cccggacagg atgccccgcc accattcctc atggtccaga taccactgaa tggtctttct
     6421 gaatccggaa gaatggtcct ggcgcggaat ccatcccaat tccctccgta ttttggaagc
     6481 gtcaatcgca tagcgcaggt catggcccgg cctgtcctgc acaaaggaga tttgctcccg
     6541 gtagctccgg ccgtccggcc ggggccgcag ttcatccaga tgggaacaca gcagctccac
     6601 cagctccaga ttggttttct cgttattgcc gccgatgttg tacgtttccc ccggttttcc
     6661 gcaggaaacg accgtcagca gcgcctcgca gtgatccccc acatacagcc agtcccgcac
     6721 gttttcccct ttgccgtata caggaatgga ttccccgccc agcgctttca ggataacgac
     6781 ggggatcagc ttttccggga actggtaggg gccgtaattg ttggaacagt tggtgatcag
     6841 cgcgggaagc ccgtacgtat cgtgccaggc gcgcaccagg tggtcgctgg aggccttgct
     6901 ggcggaatac ggggaatggg ggcgatacgg cgtggactcc gtgaacaggc cttcctttcc
     6961 cagggagccg tacacttcat ccgtggaaat gtggtggaag cggaaacgct cctttttctc
     7021 tccctccagg gaacggtaat attccagagc ggcctgaagc atggaaaaag tgcccatgac
     7081 gttggtggtc ataaaatttc ccggatcgtc aatggaacgg tccacatggg attccgccgc
     7141 caggtgcatc acccagtccg gagcgtactc ccgcaaagtg cgccgcatca gggaagcgtc
     7201 ccggacgtcg gcgtgcagaa agcggtatgc cggagaatcc gccgagccgt ccagggaatg
     7261 gatgttgccg gcataggtca gggcgtcaat attcaggact tcatggccgc gctccagcag
     7321 cagcctgacc agattggagc cgatgaatcc ggcgccgccc gtaacgataa ctttcatagc
     7381 actgttttca gggaaagatg cggcgccatc atacagcaac ggtgtttttt ttcaaaacaa
     7441 atccgcccat gccgcgggaa cgtggcggca tgggcgggaa aaagcgggtt ccgtaaccgc
     7501 tgagacggct agcagctcag ctcccggcaa aggttgatga tatcctggtt cacggagtac
     7561 tgctgcccgg caacggaatc cagggaggca tagccgtact ggccgtcatg atacaccatg
     7621 atctggttga tgtccccgtt gttcagagct tccacggcgc gggtggcaaa acgggtgccc
     7681 atcaggcggt cacgggcagt gggggaaccg ccgcgctgca catggcccag cacggtcagg
     7741 cgggtatcca tgccggcgcg ttccgtcagc cagcgggtca ggtactgcgc cacgcgggtg
     7801 ccttccgcca ccacggcaat gatgcagctt ccttcctcat tgatgacagg ctggaggcgc
     7861 ttgtaaatgg attccaggtc atacggaatt tcagggacaa tgcagatatc cgccgcgctg
     7921 caaagagcgg tggtcatcgc cagatagccg cagtcccggc ccatcgtttc aatcacgaaa
     7981 gcgcggtgat gggaacgggc cgtatcacga acggaatcaa tgcaatccag aatcacgttc
     8041 tgggccgtat ccacgcccag gcagtagtcc gtgccggcaa tgtcgttgtc aatcgtggcc
     8101 ggaataccgg caaaaggcac ttcaaaatca ttgaaaaagt cgttcagggc gcggaaggaa
     8161 ccgtccccgc ccatgatgat gagcttgtcg atgttgcgtt tcttcagatg ctcgtaggcc
     8221 tgcttgcgga attcgtagtc gaaaaagcgc ttggatcgtg aggaacggag gagagttccg
     8281 cccctgtaga tcatatcgtt ggtcagttcc cgggtggctt ccttgatttt tccatcgatc
     8341 agcccttcca agccgtcata aacaacgtac gggttcatcc caagctggcg ggcatgatcc
     8401 actgccgcct tgatcgccgg gttcattccg gcggcatcac cgccagatgt cataatggct
     8461 attccactca taagtaataa ggttaacttg tccgggggga aatgttaccg ggcggaacaa
     8521 ataatgcaag cgcggagttt ttactggcgc aataaacggc aacgcttcat gatgcggagg
     8581 catgaacgac actgcagcag ccattctgaa agccacggcc gccctccggg acggcacgga
     8641 gaagctccgg ttcgaagccc cggtccatgt tacctacaac cctctcacct atgcatgggg
     8701 gccgcatgaa cagtatgtgc gcacctacgg aaacggggaa aagagccatc tgttcctggg
     8761 catgaatccc ggccccttcg gcatggctca gacaggcgtc cccttcgggg aagtggacgc
     8821 cgtcgtcaac tggctgcaca tccgcggaga agtcgggcgg ccggaacata cccaccccaa
     8881 acgtcccgtg gagggtttca gctgccccag gtcggaagtc agcggccgcc gcctctgggg
     8941 acttttcgcg gaagctttcg gcactgcgga aaatttcttc cgtcacaatt acgtggccaa
     9001 ttactgtccg ctgatctgga tgggagccac cggagccaac atcacgccgg acaaactgcc
     9061 gacggaacag cgcgccgccg tggacgcagt atgtatggaa cacctgcttt ccctcatcac
     9121 catcctgaat ccccacacgc tggtgggggt gggtgcctac gccacgcaga agctgcggga
     9181 cgccgcctcc cggctccccg gcaaaagctt caccatcggc acgttgctcc acccaagccc
     9241 cgccagcccc atcgccaaca agctctggcc ggaacgtccc atccagcagc ttaaggaact
     9301 gggcatctta taaaaagagt agaggaggca gagtggagaa ctccgtgaca tggaaggaat
     9361 caccagagca acaggccgga aatcggcagt tcatcttgtt cccctccaaa actacatcgc
     9421 tttgaaggaa tatccggccc ccttcactgg cttgattcgc ccagggacac ggccttcctg
     9481 acggaagaaa tgcacgccgg catctaccgg gacggagaag aacttaaacg cctccccacc
     9541 tccgccacaa aatcccccct ccaaatcaat catcattctt agtctgaaaa ccctccgctc
     9601 tcccccgcag gcctttttac tggacaggga gaaaagggtt ccgtacagtt tccgcgatct
     9661 tcaagcgttc cgaatttatg gcaatttcag attacatcgt gaccattggc ctggaagtcc
     9721 actgccagat caagacaaag agcaaaatgt tctgctcctg cgagaccggc tttggctttg
     9781 aacccaatac gcgcgtgtgc cccacctgcc tggggctgcc gggcgccctg cccgtcctga
     9841 atgaattcgc cattgaacgc acgctgctga ccggcctgat gctgggatgt tccagcccgg
     9901 agatttccca atgggaccgc aagaattatt tctatccgga catggctaaa aattaccaga
     9961 cctcccagct ggatcttccg ctgtgcctgg gaggggaagt gcccctgtac ccctggtcct
    10021 atccgaatga cgtgatcaaa gccggcgtcc ctcctttccg caccgtgaag ctgacccgca
    10081 tccatctgga agaagacgcg gcgaagatta cccatcatgc caattattcc ctgatcgact
    10141 acaaccgtgc ggggtccgcc ctgatggaaa tcgtttccga gccggacatc gacaccccgg
    10201 aagaagccta cgcctacctc aagtccctcc agcagattct gaattacggg gatatctccg
    10261 acgcggacat ggaaaagggc cagatgcggt gcgatgtgaa tatttccctg cgtccccacg
    10321 ggcaggagga attgggtgcc aaggtggaaa tgaagaacat caactccatg tccgccgtcc
    10381 gcagagccct tcattatgaa atccggcgcc aggcggaaga actggacatg ggcattcctc
    10441 aaatccagtc cacccgccgc tgggacgacg aacgcggaga aagcgtcgtc atgcgaacca
    10501 aggaagacgc gcacgattac cgttacttcc cctgtccgga tctggttccc gtacgcacgg
    10561 ctccgctggt ggagaaagtg cgcggtcaga ttcccgaact gcctcaggaa cggcagaaac
    10621 gcttcatgga agaatacggc ctgagcgaat acgacgccaa cgtgctggtg ggagacctgg
    10681 aactggcaca cttttttgaa gaagcggcgc ggggaaccgc cagcggcaaa aaaatcgcca
    10741 actgggtgat taacaacatt tccgccgtgc tgaatgaaaa aggcatgcgc ccgtctgaat
    10801 gcccggtgaa acccggcgcc atccgggagc tgatcgccat tattgacgac ggcaccgttt
    10861 ccaacaacca ggccaaggac gtattcgcca aaatgtggga tgagccggcc ctcgccgcag
    10921 cgcaggccgc caagctgctg ggctttgaaa aggcggattc ctcttttctg gacggcatcg
    10981 tgcgggaagt catttccgcc aatccggaca aggtggcgga aatacagggg ggcaatgaaa
    11041 aactcctgaa ctggcttacc ggccaggtca tgaaggccgc caaaggcaaa gtcaacccga
    11101 aaatcgtgac ggaagcgctg aaaaaagccc tttccatgga gtaaccataa acaaagagcc
    11161 gcttcccttc ccggtgtcct ctccttgccc gcagggcatc acggtttgaa cagcttcctg
    11221 tttccggcca gggtgttcca tacctgcttc acttccccgg ccagcgtctg cttcatcatg
    11281 gcaagggaaa agtccgccgc ctggcccata ctgatatagg gaggcagggc aagggcgtcc
    11341 ctgtccacat gcgcgtccag cagagcggcg cccggataat tcaggaacat gttcaccatc
    11401 tggtccacct ggctggattt gtccaataaa aatcccttga tgccgacggc atccgccacg
    11461 gcggcaaaag agggattgtt caggttgatc tgccacggaa tcagcccggc cgcctgcatt
    11521 tccaattgga tgaaatccag acagtcattg ttatacacca cgattttcac cggcagcctg
    11581 tactggacaa tcgtgatgag atcccccagc agcatggaca ggccgccatc tccgcacagg
    11641 gcaattacct gccgtccggg gtattccatc tgcgccccaa tagccattgg cattgcattg
    11701 gccatggagc catgattgaa ggaagccagc gtcctcctgc caagcgtagc gcacaggaac
    11761 cttgcgctcc agatacaggg ggtgccggta tctaccgtaa atacggcgtc ggatgccgca
    11821 tgcctgttca gggcggtcgt gagctgttcc ggccggatgg gagattcctt gtcatttcct
    11881 tccatatagg cgttcatctc tttcacgtcc ctggcgtggc gtttcagggc ggcgtccaaa
    11941 tgcccggaat ccgtcttttc ctgcaccatg ttcagcaacg cctccgccgt gacggaaaca
    12001 tccccgcaaa tacccaggtc caggcggcag cggcggccca gcacttcccc gcgcctgtca
    12061 atctgcacga tttcaggttt ggtgggaatg aaattgaaat agggaaaatc cgtcccccac
    12121 aacacgagca tgtcgctttc atgcatggcc ttgtaggcat ctccccatcc cagcagcccc
    12181 gtcatgccga tgcccagggg attgtcatgt tcaaaataat cctttcccct ccatgtgtag
    12241 gcaatcggag ctttcaggcg gtgcgcgagc cggacgacct tgtcttccgc tcctgcgcag
    12301 ccggcgccgc aaaagaaagt aatgcggcgg tggtcgtcaa gcatccgtgc cagaagggat
    12361 acggattcat gttcaggaac aacattctcc cgcgtgtagg aaggaggatg caccagattc
    12421 ttcatctccg caggggcgtc cgcaatgtct ccgggcaaga cgacgacgcc tacgtcgcgc
    12481 ctggcccacg cgtgctgaat ggcggacatc aaaatgcacg gggcctggga agcttccgaa
    12541 accagttccg catatgcgct gcattccttg aaaagctgtt ccggatgcgt ggcctggaaa
    12601 tattccatgc ctacctggct ctgtgggata tgggaggcaa tggccagcac cggggcatgg
    12661 ttccggtggg cgtcaaacag gccgttgatc aaatggaggt ttccggggcc gctgctgccg
    12721 cagcatacag ccagattccc gctcagctgg gcttccgcgc tcgcggcaaa agccgccact
    12781 tcctcatgcc tcacatgaat ccattcaatg gtcttcctcc tcctcaaagc gtccgtaacg
    12841 gggttcaggc tatcccccac aat
//
LOCUS       DAOMSU010000034        12847 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_232_length_12847_cov_3.306520, whole genome shotgun sequence.
ACCESSION   DAOMSU010000034 DAOMSU010000000
VERSION     DAOMSU010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12847
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_232_length_12847_cov_3.306520"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /gene="tig"
                     /locus_tag="PK077_02735"
     CDS             <1..993
                     /gene="tig"
                     /locus_tag="PK077_02735"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRN23248.1"
                     /translation="STMTVVPEFELPEYKGIEVKVPSSEVTEADVEEALNSLAEQIAE
                     FTPVDRAAQKDDVAIIDFKTTLDGKPVAEAVGKPVGFLEGRDGQWMKVEDDQFLPGFA
                     SALEGLNAGDSKDITVTIPDTFPITELRGKELVFHATVKEVREKQLPAMDDAFAEKVL
                     PGKNLEELKTALKENLAQRKAMQIDEAKADQITEKLADMLDFNLPEAVVEREVYGILQ
                     QKMQQAMYSGNAPADMDKFVEEAREEAKQEAKRNLKVFFMLQEVAQVEKIAVTEMELY
                     NEVARQARQQKKNLKSYIRELQREGRVHGIRMSLLTAKVLDFLTKEAKVTVDEQ"
     gene            1116..1772
                     /locus_tag="PK077_02740"
     CDS             1116..1772
                     /locus_tag="PK077_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRN23249.1"
                     /translation="MFQDQSSKSTPANSYYVPMVVEQDGRGERAFDIYSRLLKDRVIF
                     IGSAIDDDVANSVIAQLLFLQMADPKKDIHVYINSPGGSVTAGLAIYDTMQFVSCDVN
                     TYCLGIAASMGSVLLAAGTPGKRFCLPNSHVMIHQVSGGAQGTASDVERTIGFMFNLK
                     KRLNGILAKHTGKTEKQIEKDADRDNYMTAEEAVAYGLVDKVLAHHSDDQDDKKKTKA
                     "
     gene            1812..3182
                     /gene="clpX"
                     /locus_tag="PK077_02745"
     CDS             1812..3182
                     /gene="clpX"
                     /locus_tag="PK077_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HRN23250.1"
                     /translation="MSGSTPHLPACSCCGKPGNKVDKLIQIAEDFYICNNCVEICVNM
                     IVKDTGLPMATRFIRGILNMEPSAYAMCQAEARKAAAADMLRETAAGPASYEGPLPTP
                     EEMCATLNQYVIGQDYAKKVLSVAVYNHYMRLRQSAVMLDDKSLDDVEIEKSNILLAG
                     PTGSGKTLLAKTLAKMLNVPFCIVDATTLTEAGYVGEDVENIILRLLQAANFDVAKAE
                     QGIIYVDEIDKIGRKTQNVSVTRDVSGEGVQQALLKIIEGTICNVPPTGGRKHPQQEY
                     IRVNTEKILFIVGGAFVGLEDIIRKRLGATQMGFGAITEQRDRKEYSEEEILAQAMPE
                     DLFSFGMIPEFVGRLPIFCPLSKLDESQLVRLLTEPKNALVKQYSKLLAMYGAKLDVL
                     PDALKAMAAEAMKRGTGARALRSIFETLMLDVMYTVPSMKNADTVTITRETVTGNKPA
                     QIHQAS"
     gene            3206..4288
                     /gene="tsaD"
                     /locus_tag="PK077_02750"
     CDS             3206..4288
                     /gene="tsaD"
                     /locus_tag="PK077_02750"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRN23251.1"
                     /translation="MPESLTVLGIESSCDETAVAILRSAGEEKAPEILSSVISSQIAI
                     HRQHGGVVPELASRNHSADLPGIIRTACREAGTAPADIDVFGATGGPGLVAALLVGNS
                     TAKALALAAGRPFVSVNHLEGHLLSPFLKRPGGPVPHLGMVVSGGHTLFVDVRGVGNY
                     RLLGRSLDDAAGEAFDKVGKMLGLPYPGGPEIDRLAAEGDPEAFSFPRALMKEHTANV
                     SFSGLKTAVLYTLPKITKNGDPHGLPRQTLRDLCASFQRAVTDVLIHKALKALRASGH
                     RTLSISGGVSCNRELRSRLKTACDREKVELVLPDFDLTTDNAAMIAYVTCLKARRGLF
                     HSLDEDVDPNLKLTEDLNRSKHSTHS"
     gene            4495..4569
                     /locus_tag="PK077_02755"
     tRNA            4495..4569
                     /locus_tag="PK077_02755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4527..4529,aa:Thr,seq:ggt)
     gene            4614..5798
                     /gene="tuf"
                     /locus_tag="PK077_02760"
     CDS             4614..5798
                     /gene="tuf"
                     /locus_tag="PK077_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRN23252.1"
                     /translation="MAKEQFQRNKPHVNVGTIGHVDHGKTSLTAAITSVLAKKGFAEA
                     RGYDQIDAAPEERERGITISTAHVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAASDGPMPQTREHILLARQVGVPAIVVYMNKCDLVDDPDLLELVEMEIRELLNE
                     YEFPGDDTPIIKGSAVKALEGDAAAEDSIMELMAAVDSYIPQPERPVDQPFLMPVEDV
                     FSISGRGTVATGRIERGVIKKMEEVEIIGIKDTQKTAVTDIEMFRKLLDEGQAGDNVG
                     LLLRGLKKEDIERGQVIIKPGTVKPHKNFKAEVYVLTKEEGGRHTPFFNNYRPQFYFR
                     TTDVTGCCTLPEGVEMVMPGDNVNLEVQLITPIAMEKAMRFAIREGGRTVGAGRISEI
                     LD"
     gene            5893..5968
                     /locus_tag="PK077_02765"
     tRNA            5893..5968
                     /locus_tag="PK077_02765"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5926..5928,aa:Trp,seq:cca)
     gene            6002..6229
                     /locus_tag="PK077_02770"
     CDS             6002..6229
                     /locus_tag="PK077_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRN23253.1"
                     /translation="MFRKISQFIAEVKGELKKTTWPWESDPKVKGFKKFRELWGSTLV
                     VLIAMVFLGAFVASFDIFLHSVVNYLIQLAI"
     gene            6259..6828
                     /gene="nusG"
                     /locus_tag="PK077_02775"
     CDS             6259..6828
                     /gene="nusG"
                     /locus_tag="PK077_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRN23254.1"
                     /translation="MPSTPEPHEQWYVLHVLSGQENSVRDRIIRKAKDAELSDFIYRV
                     EVPTELISEVRNGKKTERHSKLFPGYVYANMYLLEADGTLNEDLWYFIQQIDGVISIA
                     GSRNAPLPMRKREVEDLLPLLDAEAVAARPKVEFAVGDPVRVCEGPFQSQEGTIEEID
                     PEKGKLRVSVSIFGRATPVDLEYWQVEKA"
     gene            6895..7323
                     /gene="rplK"
                     /locus_tag="PK077_02780"
     CDS             6895..7323
                     /gene="rplK"
                     /locus_tag="PK077_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRN23255.1"
                     /translation="MAKEITKIIKLQINAGAANPSPPVGPALGQAGVNIMAFCKEFNA
                     ATQKQAGDLLPTVITVYKDKSFSFITKQPPGSLLLKKAAGIASGSGEPNKKKVATLSK
                     AKLMEVVNTKLPDLNTKDPERAARILAGQARQMGIEVEGM"
     gene            7375..8076
                     /gene="rplA"
                     /locus_tag="PK077_02785"
     CDS             7375..8076
                     /gene="rplA"
                     /locus_tag="PK077_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRN23256.1"
                     /translation="MSTKRSKRYQEAAKLVIPNKNYSITEAVETMKSFPAPKFDPTVT
                     VSFHLTVDPRKSDQMVRGSVSLPNGTGKNVRVLVFAQGDAAKVAQEAGAEFVGFEDLI
                     KKVQGGFVDFDTAIATPDAMTEVRKVARVLGPRGLMPNPKTGTVTDDTAKAVKEVKAG
                     RVDYKVDKNANISAAVGKLSFSNESIAENIAALIDSVVKARPASAKGAYIESVTVSCA
                     MCPGLPVDTASIAKL"
     gene            8098..8601
                     /gene="rplJ"
                     /locus_tag="PK077_02790"
     CDS             8098..8601
                     /gene="rplJ"
                     /locus_tag="PK077_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRN23257.1"
                     /translation="MNPDKRIIIDDLTARVNASPFLIVVDYTSITVPEFTNLRSALAA
                     CGAQCHVAKNNFMRTALTDAGLPDIGEHLVGQTAFVTGASDVAGAAKAINTFAKASKK
                     AEYKVAILDGAILSADEIRAIGELPPRDQLLAKLLGTINAAGSALARVIQAYVDKENG
                     GSEEPAA"
     gene            8760..9134
                     /gene="rplL"
                     /locus_tag="PK077_02795"
     CDS             8760..9134
                     /gene="rplL"
                     /locus_tag="PK077_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRN23258.1"
                     /translation="MADINKIAEELGTLTILEAADLVKLLEEKWGVSAAAPVAAAGAA
                     AAPAEAEEEKTEFNVVLTEAGANKIAVIKAVREVKAGLGLVDAKKLVEGTPAVILEAV
                     SKDEANAAKAKLEEAGAKVDVK"
     gene            9336..>11420
                     /gene="rpoB"
                     /locus_tag="PK077_02800"
     CDS             9336..>11420
                     /gene="rpoB"
                     /locus_tag="PK077_02800"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRN23259.1"
                     /translation="MSKRLYFGNIKEVIEPPNLIEIQLQSYFDFLQQDTPAAARKNIG
                     LQGVLKEIFPIKSYDENIELDFVSYDIEQPKMSDYEAIRAGETYSAALQVTFKLKSDN
                     ESKEETVYMGELPMMTNRGTFVINGAERVIVSQLHRSPGICFESAQHLNGKLLHSFRI
                     IPDRGSWLEVQFDTNDLLYVYLDRRRRRRKFLATTFMRYLGFKTDRDIVSQFYNIRTL
                     PLSEDMTEEDLHNLVAVDTIKDKDLVLAKAFEQLNMGVVRQLLQFGIKKIDVIDQSED
                     DVLIKTLKKDPAHDEESALKEIYKRLRPGDPATAAQARTLLKRLFDDPKKYDLTRVGR
                     YKINQKLGLDTSLDQRLMTAEDFLAALKYLLRLKKGEGMVDDIDHLGSRRVRAVGELM
                     ANQCRVGLARTERLVKERMTLIDQNIEGVTPSKLINPKALSAVVRDFFGRSQLSQFMD
                     QINPLAELTHKRRLSALGPGGLNRDRAGFEVRDVHPSHYGRICPIETPEGPNIGLINS
                     MCTYARINEFGFIETPYRKVENGRVTNTIEYVTADQEEGYLIAQANNPLDEQGNFTTS
                     RVTAREKGEFIEVDPADVHYMDVSPKQLVSIAAGLIPFLEHDDANRALMGSNMQRQGV
                     PLMVAESPYVGTGIEGKCARDSRSVVLAEADGIVASATAEVIITTKDGELPVRPEVFL
                     SDPDSVRTDRDNG"
     assembly_gap    11421..11430
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11431..>12847
                     /gene="rpoB"
                     /locus_tag="PK077_02805"
     CDS             <11431..>12847
                     /gene="rpoB"
                     /locus_tag="PK077_02805"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420082.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRN23260.1"
                     /translation="FMRSNAGTCINQRPIVRRGDKIKTGDVLADGPNTDQGELALGRN
                     VLVAYMPWNGYNFEDAIVISEKTVKEDTFTSIHISEFEVQARDTKLGPEEITRDIPNA
                     GDEALKNLDHDGVIRIGAEVKPGDILVGKITPKSETELAPEERLLRAIFGEKAAEVKD
                     TSLRVPSGCTGIVMDVRISSTGSGHHRGDLVVDSAEKKKQFKKINDEHKKKKEQLIDQ
                     LTKKLSDILLGEKIPLDVVNEQTGEIIIPANRKITKTLLRKLALVHDHIEIEPSPIRN
                     KILEIITSFEGRFTELDDEREHRLDQMESGDESEPGGLKEVKVYIAAKRKLGVGDKMA
                     GRHGNKGVVAKIVPEQDMPFLADGTPVDIVLNPLGVPSRMNVGQVLEAHLGIAARALG
                     FKVATPVFDGISEETIWNYMSEAKKVDGFTWIGDGKDGTVGGKSTLYDGLTGEPFHNP
                     VVVGQTYMLKLNHLVADKIHAR"
BASE COUNT         3142 a         3967 c         3264 g         2464 t
ORIGIN      
        1 agcacgatga ccgtagttcc cgaatttgaa ttaccggaat acaaaggcat cgaagtcaag
       61 gttccctctt ccgaagtaac ggaagcagac gtagaggaag ctctgaactc cctggctgaa
      121 caaatcgcgg aattcacccc tgtggaccgc gccgcccaaa aagacgacgt ggcgattatc
      181 gacttcaaaa ccacgttgga cggcaagccc gtagcggaag ccgtaggcaa acccgtaggc
      241 ttcctggaag gccgtgacgg ccagtggatg aaggtggaag acgatcaatt cctgcccggc
      301 ttcgcctccg ccctggaagg cctgaatgcc ggtgacagca aggatatcac cgtcaccatt
      361 ccggatacct tcccaattac ggaactgcgc ggcaaggaac tcgtattcca tgccactgtg
      421 aaagaagtac gggaaaaaca gcttccggcc atggatgatg cttttgcgga aaaagttctt
      481 cccggcaaaa acctggaaga attgaaaacc gccctgaagg aaaacctggc ccagcgcaaa
      541 gccatgcaaa ttgatgaggc caaggccgat cagatcacgg aaaagctggc ggacatgctt
      601 gacttcaacc ttccggaagc tgtcgtagaa cgtgaagtgt acggcatcct ccagcagaaa
      661 atgcagcagg ccatgtacag cggaaacgcc ccggcggaca tggataagtt tgtggaggaa
      721 gcccgggaag aagccaaaca ggaggccaag cgcaacctta aggtcttctt catgcttcag
      781 gaagtggcgc aggtggaaaa aattgccgtc acggaaatgg agctttacaa cgaagtggcc
      841 cgccaggccc gccagcagaa aaagaacctc aaatcctaca ttcgcgaact ccagcgcgaa
      901 ggccgcgtgc atggcatcag aatgagcctg ctgacagcca aggttctgga cttcctgaca
      961 aaagaggcca aagtaacggt agacgagcag taacccctcc tctgcacaaa acaccttacc
     1021 agggggagtt ggcgcacaca tgcccgactc ccccgttttg catcttttgg acgggtaacg
     1081 tacattgaaa aaacaacatc caatcttcca taaacatgtt tcaagaccaa tcttccaaat
     1141 caacccccgc aaactcctac tatgtgccca tggtagtgga gcaggacggc cgcggcgaac
     1201 gtgcctttga catttactcc cgtctcctga aagaccgcgt tatcttcatc ggctccgcta
     1261 ttgacgacga tgtggcgaat tccgtcattg cccagcttct gttccttcag atggctgacc
     1321 cgaaaaaaga catccacgtc tacatcaact cccccggagg atccgtcacg gcgggtctcg
     1381 cgatttacga tacgatgcaa ttcgtctctt gtgacgtaaa tacatattgc ctgggcatag
     1441 cggcctccat gggttccgtc cttctggctg cgggaactcc cggcaaacgc ttctgcctgc
     1501 cgaactccca cgtaatgatt caccaggttt ccggaggtgc ccagggaacc gcttcagatg
     1561 tggaacgcac catcggcttc atgttcaacc tcaaaaaacg cctcaacggc attctggcca
     1621 aacataccgg taaaacggaa aagcaaattg aaaaggatgc tgaccgcgac aactacatga
     1681 ctgccgagga agccgtggcc tacggcctgg tggataaagt tctggcgcat cactctgatg
     1741 accaggacga caagaaaaaa accaaggcct gaccctttcc ctccgccatt tctctctcta
     1801 cctgaaaaac catgtccggt tccacccctc atcttcctgc ctgttcctgc tgcggcaagc
     1861 ccgggaacaa ggtggacaaa ctcatccaga ttgcggaaga cttttacatc tgcaacaatt
     1921 gcgttgaaat ttgcgtcaac atgattgtga aggacacagg ccttccgatg gcgacccgct
     1981 tcatccgcgg catcctgaac atggaaccct ccgcctacgc catgtgccag gcggaggccc
     2041 gcaaagcggc agccgcggac atgctcaggg aaacggcggc cggaccggcc tcctatgaag
     2101 gcccgctgcc cacaccggaa gaaatgtgcg ctacgctcaa ccagtatgtc atcggtcagg
     2161 actacgccaa aaaagtgctt tccgtagccg tgtacaacca ctacatgcgc ctgcgccaaa
     2221 gtgctgtcat gctggacgac aagtccctgg acgatgtgga aattgaaaaa tccaacattc
     2281 tgctggccgg ccccaccggc tcaggaaaaa ccctgctggc gaaaacgctg gcgaaaatgc
     2341 tcaacgtccc attctgcatt gtggacgcca ccacgctgac ggaagccggt tacgtagggg
     2401 aagatgtgga aaacatcatc ctgcgcctgc tccaggctgc caactttgac gtagcgaaag
     2461 cggaacaagg catcatctac gtagatgaaa tcgacaaaat cggacgcaaa acacagaatg
     2521 tctccgtcac gagggacgtc tccggggaag gcgtgcagca ggctctgctg aaaatcattg
     2581 aaggcaccat ctgcaatgtt cctcccaccg gaggccgcaa gcacccgcaa caggaataca
     2641 tccgcgtcaa tacggaaaaa atcctcttca ttgtgggcgg cgctttcgtc gggctggaag
     2701 acatcatccg caaacgcctc ggcgccaccc agatgggatt cggagccatc acggaacaac
     2761 gtgaccgcaa ggaatactcg gaagaggaaa tactggcaca ggccatgccg gaagacctct
     2821 tctccttcgg catgattccg gaattcgtgg gacgcctgcc catcttctgt ccgctctcca
     2881 agctggatga aagccagctc gtccgccttc ttacggaacc caaaaatgcc ctggtcaagc
     2941 aatattccaa actgctcgcc atgtacggcg ccaaactgga cgtgctgccg gacgccctga
     3001 aagccatggc cgccgaagcc atgaaacgcg gcacgggagc ccgcgctctg cgttccatct
     3061 ttgaaaccct catgctggac gtcatgtaca cagtgcccag catgaaaaat gcggacaccg
     3121 ttaccattac cagggaaacg gttaccggca acaagccggc ccaaatccac caggcatcct
     3181 aacctcccct ccctgttctt cctccatgcc ggaatccctt accgttctgg gaatagaatc
     3241 ctcctgtgat gaaacggcag tcgccatcct gcgttctgcc ggagaggaaa aagctccgga
     3301 aatactctcc tccgtcatct cctcccaaat tgccattcac cgccagcacg gcggcgtagt
     3361 gccggaactg gcttcccgca accattcagc ggatcttccc ggaatcatcc ggaccgcttg
     3421 ccgcgaagcc ggaacagctc ctgcggacat tgacgtcttc ggcgctacgg gaggccccgg
     3481 cctggtagct gcgcttctgg taggcaacag cacggccaag gctctggctc tggcggcggg
     3541 caggcccttc gtctccgtca atcatctgga aggccatctg ctttccccct tcctcaaacg
     3601 ccccggcggt cccgttcccc atctgggcat ggtcgtttcc ggaggccaca ccctttttgt
     3661 ggatgtgcgc ggcgtaggga actaccgcct gctgggccgc tctctggacg acgcagcagg
     3721 ggaagccttt gacaaggtag gcaaaatgct aggccttccc tatcccggag ggccggaaat
     3781 cgaccgcttg gcggcggaag gcgacccgga agccttttct ttcccccgtg ccctgatgaa
     3841 agagcataca gccaacgtat ctttctccgg cctgaaaacg gccgttctct atacactgcc
     3901 caaaattacg aaaaacggcg atcctcacgg cctgccccgg caaactctgc gcgacctctg
     3961 cgcttctttc cagcgggccg tgacggacgt cctgattcac aaggcgctga aagccttgcg
     4021 cgcctccggt caccgcaccc tttccatctc cgggggcgtc tcctgcaaca gggagctgcg
     4081 ttcccgcctg aaaaccgcct gtgaccgtga aaaagtggaa ctggttctcc cggacttcga
     4141 cctgacgacg gataatgccg ccatgatcgc ttatgtcacc tgtctcaaag cccgcagagg
     4201 actgttccac tctctggatg aagacgttga ccccaatctt aaattgacgg aggatttaaa
     4261 cagatccaaa cattcaacac attcctgata aatatattat acatggactc cctgtatttg
     4321 cggcataaag ctgaaaaaat gttcatgttt tcattgacat cccgcatcta aaaacgtaac
     4381 atagcgtccg cttttcgtca ggcaacacgg gaggatgcta tttatccgct agaaccgcag
     4441 gtttgggcgg atttacgact tctctaggag cagcgaaagg cggaagaagt ctatgcttct
     4501 gtagctcagg ggtagagcgc atccttggta aggatgaggt cgcgggttca attcccgcca
     4561 gaagctccat cttcaagcta gacccatagc agcccaaacc taaacataat attatggcta
     4621 aagagcaatt ccagcgcaac aagccccacg tgaacgtggg caccatcggt cacgttgacc
     4681 acggcaaaac ctcccttacc gccgccatta cttcagtgct cgctaagaag ggtttcgccg
     4741 aagcacgcgg ctatgatcag attgacgccg cccccgaaga acgcgaacgc ggcatcacca
     4801 tctccacggc tcacgttgaa tacgaaacgg aaaaccgcca ctatgcccac gttgactgcc
     4861 ccggacacgc cgactatgtg aaaaacatga tcaccggcgc tgcccagatg gacggcgcca
     4921 tccttgtggt tgccgcttcc gacggcccga tgccgcagac ccgcgaacac atcctccttg
     4981 cccgccaggt gggcgttccc gccattgtcg tttacatgaa caagtgcgac ctggttgacg
     5041 atcccgacct tcttgaactc gtggaaatgg aaatccgcga acttctcaac gaatacgaat
     5101 tcccgggcga cgacaccccc attatcaagg gttccgccgt taaggctctg gaaggcgatg
     5161 ccgctgccga agattccatc atggaactta tggccgccgt tgactcctac atcccgcagc
     5221 ccgaacgccc tgtggaccag cccttcctca tgcctgtgga agacgtgttc tccatctccg
     5281 gccgcggcac cgtggctacc ggccgtatcg aacgcggcgt catcaagaag atggaagaag
     5341 tggaaatcat cggcatcaag gacacccaga aaaccgccgt tacggacatc gaaatgttcc
     5401 gtaagctgct tgacgaaggc caggccggcg acaacgttgg tctgcttctc cgcggcctga
     5461 agaaggaaga catcgaacgc ggccaggtaa tcatcaagcc cggcactgtg aagccccaca
     5521 agaacttcaa ggctgaagtt tacgtcctga ccaaggaaga aggcggccgt cacactccgt
     5581 tcttcaacaa ctaccgcccg cagttctact tccgtacaac ggacgtgacc ggttgctgca
     5641 cccttcccga aggcgtggaa atggtgatgc ccggcgacaa cgtcaacctg gaagtccagc
     5701 tcatcactcc gatcgccatg gaaaaagcca tgcgattcgc tatccgcgaa ggcggccgca
     5761 ccgtgggtgc tggtcgtatc agcgaaatcc ttgactaagt tcctcaggaa cttgtagagg
     5821 aatctacatc aggtttaatg agggggcctc cccgcaggcc ccctcatttc ccacgaaaga
     5881 agagactcca acaggtcagt agttcaattg gtagagcgtt ggtctccaaa accaaaagtt
     5941 gggggttcga gtccctcctg acctgccact tctttccaaa tacaccaccc caccttgaag
     6001 catgtttcgc aagatttctc aattcatcgc cgaagttaag ggcgaactta agaagacgac
     6061 ctggccctgg gaaagcgacc ccaaggtgaa aggtttcaaa aaattccgcg aactctgggg
     6121 ttccactctc gtcgtactga ttgccatggt attccttggc gcgtttgttg catcgttcga
     6181 catctttctt cacagcgtgg tcaactatct catccagttg gcaatctagt tttactctcc
     6241 ccacttcttt ctccgatcat gcccagtacc ccagagcctc acgaacaatg gtatgtcctt
     6301 cacgtcctct ccggacagga aaacagcgtt cgtgaccgca tcatccgcaa ggcgaaggac
     6361 gccgagcttt ccgacttcat ttaccgggtg gaagtcccca cggaacttat ttccgaagtg
     6421 cgcaacggca aaaaaacgga acgtcacagc aaactctttc ccgggtatgt ttatgccaac
     6481 atgtacctgc tggaagcgga cggcaccctg aacgaggacc tttggtactt tatccagcaa
     6541 attgacggtg tcatcagcat tgccggctcc cgcaacgccc ccctccccat gcgcaagcgc
     6601 gaggtggaag accttctgcc cctgttggat gcggaagccg tcgccgcccg tcccaaggtg
     6661 gaattcgccg tcggcgaccc tgtgcgcgtg tgcgaaggcc cgttccagag ccaggaaggc
     6721 accatcgaag aaatcgaccc ggaaaagggc aagctgcgtg tcagcgtctc catttttggc
     6781 cgtgccacgc ctgtggactt ggaatactgg caggtggaaa aagcctgaat cttctttgcg
     6841 tctcccccca tcagccccct tcccgtttat tttttccgct aacaaagcaa aaccatggca
     6901 aaagaaataa ccaaaatcat caagctccag atcaatgccg gagccgctaa cccctccccg
     6961 cccgtgggtc cggcacttgg tcaggccggt gtaaacatca tggcattctg caaggagttc
     7021 aacgccgcca cgcaaaagca ggccggcgat ctcctgccga ccgtcatcac ggtttacaag
     7081 gacaagtcat tttctttcat caccaagcag cccccgggca gcctgcttct caagaaggcc
     7141 gccggcatcg cctccggctc cggcgaaccg aacaaaaaga aggtcgccac tctttccaag
     7201 gccaagctga tggaagttgt caacaccaaa ctccctgacc tcaacacgaa agaccctgaa
     7261 cgcgccgccc gcattcttgc cggccaggct cgccagatgg gcattgaagt tgaaggtatg
     7321 taacactcac cgcaggagga ttaaaccagt ccgaacgcac tgccaaatac acacatgtct
     7381 accaaacgca gcaagcgata tcaagaagca gccaagcttg ttatccctaa caagaactac
     7441 agcatcacgg aagccgtgga aaccatgaaa tccttcccgg caccgaaatt cgacccgacc
     7501 gtcaccgtct ccttccacct gactgtggac ccccgcaaat ccgaccagat ggttcgcggc
     7561 agcgtttccc tgcctaacgg cacgggtaaa aacgtccgcg tcctcgtctt tgctcagggc
     7621 gatgccgcca aggtcgccca ggaagccgga gccgaattcg tggggtttga agatctcatc
     7681 aagaaagtcc agggaggatt tgttgacttt gatacggcca ttgccacccc cgacgccatg
     7741 acggaggttc gcaaggttgc ccgtgtactg ggtccgcgcg gcctgatgcc caacccgaaa
     7801 accggcaccg tcacggacga tacggccaag gctgtgaagg aagtaaaggc cggccgcgtt
     7861 gactacaagg ttgacaagaa cgccaatatc tccgctgctg taggcaagct gtctttctcc
     7921 aacgaaagca tcgccgaaaa catcgctgcc ctgattgatt ccgtcgtcaa ggcccgcccg
     7981 gcttccgcca agggcgccta catcgaatct gtcaccgttt catgcgccat gtgccccggt
     8041 ctgccggttg acactgcttc catcgccaaa ctgtaacccg aaccgattta gcacaccatg
     8101 aatcctgaca agcgtatcat cattgacgac ctgaccgctc gcgtcaacgc ctccccgttc
     8161 ctgatcgtcg tggactacac ctcgatcacc gtgcctgagt tcaccaatct gcgttccgcc
     8221 ctggccgcct gcggcgctca gtgccacgta gccaagaaca acttcatgcg cacggctctg
     8281 acggatgccg gccttcccga cattggggaa cacctcgtcg gccagaccgc tttcgtgacc
     8341 ggcgcatccg acgtagcagg cgccgccaag gccatcaaca cctttgccaa ggcttccaag
     8401 aaagccgaat acaaggtggc gatcctggat ggagccattc tttccgcgga tgaaatccgc
     8461 gccatcggcg aactgcctcc ccgcgaccag ttgcttgcca aactccttgg cactatcaac
     8521 gcggccggtt ccgctcttgc ccgagttatt caggcatatg tggacaaaga aaacggcggc
     8581 agcgaagaac ccgccgccta ataccaaccc cttgaccgga cggtccggtt gcactctagc
     8641 caaatgtgtg aaaatcggac ccgactctcc tacaggttcc ttgtcggaac tccggccgga
     8701 gaactgaaat gccaagttgg ctcttggcgc gactcgaaac caaactagaa aacaatacaa
     8761 tggctgatat taacaaaatc gctgaagaac tcggcaccct taccatcctg gaagctgccg
     8821 acctcgtcaa gcttctggaa gaaaagtggg gcgtttccgc cgctgctccc gtagctgccg
     8881 ccggcgctgc cgccgctcct gccgaagctg aagaagaaaa gaccgaattc aacgtcgtcc
     8941 tgacggaagc cggcgccaac aagatcgccg taattaaagc cgtccgcgaa gtgaaggccg
     9001 gcctcggcct ggtggacgcc aagaaactgg ttgaaggcac tcccgccgtc atcctggaag
     9061 ctgtctccaa ggacgaagct aacgccgcca aggccaagct ggaagaagct ggcgccaagg
     9121 tagacgtcaa gtaagctttt tacttcccgc gcaacaggag aaaagccgtt tttctcctgt
     9181 tgcgcttcct cgtcttcaca tcccgcgtga ccgactagag gccttttatt tcgtaaaacg
     9241 cttccagtcg gttccgtccc ccagggaaag aaccgcccaa cccggcatcc gggtttttca
     9301 ggatgcaaac cataacatct ccgccacagc agcccatgtc aaagcgactc tacttcggga
     9361 atatcaagga agtcatcgaa cctccgaacc ttattgagat tcaacttcaa tcatacttcg
     9421 attttctaca acaggacacg ccggccgcgg ccaggaaaaa catcgggtta caaggcgttc
     9481 tgaaggaaat ctttcctatc aagagctatg acgaaaacat tgagctcgac ttcgtctcct
     9541 atgacattga acagccgaag atgagcgatt acgaggccat ccgcgcgggg gaaacctaca
     9601 gcgccgccct tcaggtcacc ttcaagctca aatccgacaa cgagtccaag gaagaaacgg
     9661 tctatatggg agaactcccc atgatgacca accgcggcac ctttgtcatc aacggcgccg
     9721 aacgcgtcat tgtgtcccag ctgcaccgtt ccccgggcat ttgctttgaa agcgcccagc
     9781 atctgaacgg aaaactgctc cactccttcc gcatcatccc ggaccggggc tcctggctgg
     9841 aggtccagtt tgacaccaac gacctgcttt acgtttacct tgaccgccgc cgccgccgcc
     9901 gcaaattcct tgcgacgacg tttatgcgct acctgggctt caaaacggac cgtgacatcg
     9961 tcagccagtt ctacaacatc cgcacgcttc ccctgagcga agacatgacg gaagaagatc
    10021 tgcacaacct cgtggctgta gacacgatca aggacaagga tctggttctt gccaaggctt
    10081 tcgaacagct caacatgggt gtggttcgcc agctcctcca gttcggcatc aagaaaatcg
    10141 acgtcattga ccagagcgag gacgacgtgc tgatcaaaac cctgaaaaag gatcccgctc
    10201 acgacgaaga atccgccctc aaggaaatct acaaacggct gcggcccgga gaccccgcta
    10261 cggcggctca ggcgcgcacc ctgctcaaga ggctttttga cgatcccaaa aaatatgacc
    10321 tcacccgcgt gggacgctat aaaatcaacc agaagctcgg tctggacacc agcctggacc
    10381 agcgcctgat gacggcggag gatttcctgg ccgccctcaa atacctcctg cgtctcaaga
    10441 agggtgaagg catggtggac gacatcgacc acctgggcag ccgtcgcgta cgcgccgtag
    10501 gcgaacttat ggccaaccag tgccgcgtag gtctcgcccg caccgaacgc ctggtcaagg
    10561 aacgcatgac cctcattgac cagaacattg aaggcgtcac gcccagcaaa ctcatcaatc
    10621 cgaaggccct cagcgccgtc gtgcgcgact tcttcggccg ctcccagctc tcccaattca
    10681 tggaccagat caaccccctc gcggaattga cgcacaagcg ccgtctttcc gccttggggc
    10741 ccggcggcct gaaccgtgac cgggccggtt ttgaagtccg ggacgtgcat ccctcccact
    10801 acgggcgcat ttgccccatt gaaacgccgg aaggtcccaa catcggcctg atcaactcca
    10861 tgtgtaccta cgcccgcatc aatgaattcg gattcattga aacgccttac cgcaaggtgg
    10921 aaaatggcag ggtcaccaat accatcgaat acgtcaccgc cgatcaggaa gaaggctatc
    10981 tcatcgccca ggccaacaac ccgctggacg aacagggcaa cttcacgacc tcccgcgtaa
    11041 ctgcccgcga aaaaggcgaa ttcatcgaag tggacccggc ggatgtgcat tacatggacg
    11101 tatctcccaa gcagctcgtt tccatcgccg ccggtcttat ccccttcctg gaacatgacg
    11161 acgccaaccg cgccctgatg ggttccaaca tgcagcgcca gggtgtgcct ttgatggtgg
    11221 cggaatctcc gtatgtggga accggcattg aaggcaagtg cgccagggac tcccgttccg
    11281 tcgttctggc ggaagcggac ggcatcgtgg cctccgccac ggctgaagtc atcatcacca
    11341 cgaaagacgg agagttgccc gtgcgcccgg aagtgttcct gtccgatccc gacagtgtgc
    11401 gcacggaccg cgacaatggc nnnnnnnnnn agttcatgcg ttccaatgct ggcacctgca
    11461 tcaaccagcg tccgatcgtg cgccgcggcg acaaaatcaa aaccggcgat gtgctggcgg
    11521 acggcccgaa tacggaccag ggtgaactgg ccctcggccg caatgttctg gtggcctata
    11581 tgccgtggaa cggttacaac ttcgaagacg ctattgtcat ctccgaaaaa accgtgaagg
    11641 aagacacctt cacctccatc cacatctccg agtttgaagt gcaggcccgt gataccaagc
    11701 tgggcccgga agaaatcacc cgtgatatcc cgaacgccgg tgatgaagcc ctgaaaaacc
    11761 tggaccatga cggtgtcatc cgcatcggcg cggaagtgaa gcccggcgac attctcgtcg
    11821 gcaaaattac gcctaaatcg gaaacggaac tggccccgga agaacgcctc cttcgcgcca
    11881 tcttcggtga aaaggccgca gaagtgaaag atacatccct ccgcgttccc tccggctgca
    11941 ccggcattgt gatggacgtg cgcatttctt ccacaggctc cggccaccac cgcggcgacc
    12001 tcgtcgtgga cagcgcggag aagaaaaagc agttcaagaa aatcaacgac gaacacaaga
    12061 agaagaaaga gcagcttatt gaccagttga ccaagaagct ctccgacatt cttctgggcg
    12121 aaaaaatccc gttggacgtc gtcaacgaac agaccggtga aatcatcatc ccggccaacc
    12181 ggaaaatcac caagaccctg ctgcgcaagc tggccctggt tcacgaccac atcgaaatcg
    12241 agcccagccc gatccgcaac aagattctgg aaatcatcac ctcctttgaa ggccgcttta
    12301 cggaactgga tgacgaacgg gaacaccgac tggatcagat ggaatccgga gacgaatccg
    12361 aacccggcgg actgaaagaa gtcaaagtat acatcgccgc caagcgcaag ctcggtgtgg
    12421 gtgacaagat ggccggccgc cacggcaaca agggcgttgt cgccaaaatc gttccggaac
    12481 aggatatgcc tttcctcgcg gacggcactc cggtggacat cgtgctgaac cccttgggcg
    12541 tgccttcccg aatgaatgtg ggacaggtgc ttgaagccca cctcggcatc gctgccaggg
    12601 cccttggctt caaggtggct accccggtgt tcgacggcat cagtgaagaa accatctgga
    12661 attacatgtc cgaagccaag aaggtggacg gtttcacctg gatcggtgac ggcaaggacg
    12721 gcaccgtggg agggaagagt actctttatg acggcctgac cggcgaacct ttccataacc
    12781 cggtggtggt aggccagacc tacatgctca aactgaacca cctggtggcg gacaagattc
    12841 acgcccg
//
LOCUS       DAOMSU010000035        12818 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_235_length_12818_cov_3.091828, whole genome shotgun sequence.
ACCESSION   DAOMSU010000035 DAOMSU010000000
VERSION     DAOMSU010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12818)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12818)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12818
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_235_length_12818_cov_3.091828"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1273)
                     /locus_tag="PK077_02810"
     CDS             complement(<1..1273)
                     /locus_tag="PK077_02810"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRN23261.1"
                     /translation="MQAYIPEFILAGLAMLLLLAETFCKKTPKFIFGLIGAAGTLAML
                     PFYMEGLYDNIYIILALVATAVTLLLSVDFRAVINLSSNDSKSQDGTGEFYILPLLAC
                     VGITSLCKASNLVELFVSLEVLTLSSFIMVGYFRRNLGSTEAGIKYLILGAVSTGFLV
                     FGLAWYFGITGTFIYNETIVSHALAGQTAPAMYLALAMLLLGTAFKIGAVPMQLWIPD
                     VYQGAPTPVTAFLSVASKVAGFALLGIILAPFAVLPPVEFVVALMAAATLLVGNLGAI
                     PQTNLKRMMGYSSIAQAGFILPLFIGTVDGQLAPNAPFYLAVYLVMTFGAFFALAMIR
                     IQRGSEEISAFRGLGKTNPRLALAVTIMFASLAGVPLTAGFFAKMISFVHVINTGLYL
                     DWMLPVMIVCAASGFYYYFKVIRSMYWDKPSE"
     gene            complement(1324..2748)
                     /locus_tag="PK077_02815"
     CDS             complement(1324..2748)
                     /locus_tag="PK077_02815"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRN23262.1"
                     /translation="MLIWLVLIPLIAAALIGLCKAPARPTALLSASLTLALGIWALVS
                     FDGCSSCWSRFEGMDLQLTLAPALSKVMLLLTILVTFATVLGTNPPQGGEASWYNSAL
                     LISAGATGAFLSDNIISFFAFHELALIPTFVMIGLYGRGDRRTTAWRATLYLGLASMV
                     LLAALLMIGTQAGFTFSGLKDFMASGRELAHAELIGALLIAGFGTLISLFPFHSWAAP
                     AYASAPAPVAMMHAGVLKKFGLYGLFMFQPLMETGFLPWTNILLVLLVCNVIWVGYVT
                     VNQKRLDLLLGNSSVMHMGYIFLAFAALVVSGSAEANPWALKGAALLMLAHGLTIALL
                     FLLCGQIEKQTGTLEINSLGGLSTRLPRMAFVFGLAGMASIGLPGLANFPGEFMVFFS
                     GFAGFSNGFGPVQIATILCLWGLVIGAVYMLRAYRNIFQGDLSKASAYATGLQPSERA
                     ANYFLVIALAVFGFFPTLVLQFFS"
     gene            complement(2771..4603)
                     /gene="nuoL"
                     /locus_tag="PK077_02820"
     CDS             complement(2771..4603)
                     /gene="nuoL"
                     /locus_tag="PK077_02820"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRN23263.1"
                     /translation="MFNIDIQYTWLLLFLPLVVAAFDWFLLTKRPDVAALTSTFSCLA
                     TFVLSLGLLDRTGSDSFSWLPISDIFSVDLGFVLDPLSSRMMLVVTGIGLLVHVFSLA
                     YMADDKAKTRYFACLSLFMFSMTGIVLANNIAMTFIFWELVGLSSYLLIGHWYTRDTA
                     ANAAKKAFICNRVGDFGFLIGILTLWALTNTLDFSLMEIPAGISENLLNITVLCLFCG
                     AVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMIAAGVYMMVRVQYSIGIEAFPALAC
                     NVIAGIGAITAVIAAFMATQQNDIKRVLAYSTLSQLGYMVMALGLLAGEAAMFHLFTH
                     AWFKALLFLGAGAIIFACHHEQDIWKMGGIMKRMKLTSLTFIIATMALIAIPFTSGFF
                     SKEAILEAALRKNPVFFWIGAGVALLTTFYMMRVIFVVFFGKSRSHSSEHASEVGGLM
                     LVPLLILAVLALISGYGFIADRLVPFNGFVSESFHMGMPFYVSMGALVLGILLAAAFY
                     AGSPASDKLSGNAVSRALANRLYIDLFYDKVLVRGVQGVLAAIIDFMDQFIISGLIVG
                     GLARLTAAIGSLLRRLQSGNMAAYTALFGIGLLLVIYFTVFYSC"
     gene            complement(4627..4950)
                     /gene="nuoK"
                     /locus_tag="PK077_02825"
     CDS             complement(4627..4950)
                     /gene="nuoK"
                     /locus_tag="PK077_02825"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRN23264.1"
                     /translation="MTMIPLTHYLILSGVLFAIGLMGVIVRRDIIVIFMCLEMMLSAA
                     NLSLVAFSRAQGTMGLPNYDGQALSIFILTIAAAEVAIGLALIVSLYRARRTASTQDL
                     NTLKD"
     gene            complement(4970..5680)
                     /locus_tag="PK077_02830"
     CDS             complement(4970..5680)
                     /locus_tag="PK077_02830"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRN23265.1"
                     /translation="MNIFASDILFYVFGALAVVLSLMVVFMRNPVSSAMMMALSFGAT
                     AAVMIGLGAHFLGILQILVYAGAIMVLFAFIIMLLNVKQETSPFSRPFSVAIGVIIAC
                     LFLGQLIGIIYSLPGAKETHRCPMASAEQAWNDLKIGQEDAAPSKTGMETPACSLRNN
                     GAPSVILPPLSPQCSAHLYPEGTTIREEIDNGEFPDTALLGRTLFDKYNRSLVIAGLA
                     LLVASIGVVVLSRRPSGK"
     gene            complement(5709..6272)
                     /locus_tag="PK077_02835"
     CDS             complement(5709..6272)
                     /locus_tag="PK077_02835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HRN23266.1"
                     /translation="MAFKTIKRPKISLGERFYLQSILGGLWLTIRHLVLALLGKSRNK
                     KELAGSGIGVTMQYPEARWDKHLPKYYRGAPVLVKGEDGRERCVSCQLCEFICPARAI
                     TITPGPIQEGPWGKVEKAPREFQIDMLRCIYCGMCEEACPEQAIFMSHNYLFTPTDKT
                     QAIHNKAKLYELGGTRKGLVNKWNQYK"
     gene            complement(6307..7560)
                     /locus_tag="PK077_02840"
     CDS             complement(6307..7560)
                     /locus_tag="PK077_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRN23267.1"
                     /translation="MIDSILTFCDEVLSNPVWFYIITLLIKIVICFAITILFIPVCVY
                     IERRVAAWIQDRVGPNRAGIPLSIFRRFGMKSDLPFKKTLDYFGLPHDGKIANLCRFF
                     RLDRDIPIFGLVQPMVDGGKLFLKQDFTPPFVRRVYFWIAPILVLAPPLMTAAVVPFA
                     GDLQTSYGNVNMAVANLSVGPLWMFAISSLSVAGWSSNSKFPFLGGVRSSAQMISYEI
                     SMGLSIIPVLMIYSTLDLSNIVEYQAANGWLLLPVWGEGLSWQRWILLVPVAISFIIF
                     LTSIFAEANRTPFDMSECETDLVGGYHTEYSSMKFAQFFMGEYAAMVVGSSLVITLFL
                     GGWSIGFGLDGWLNENVANWVAVICQLIAYVLKLLFFAFFFVWVRWTLPRFRYDQVMK
                     LGWMVFFELAVINIFLTAAILYFVK"
     gene            complement(7596..9332)
                     /locus_tag="PK077_02845"
     CDS             complement(7596..9332)
                     /locus_tag="PK077_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRN23268.1"
                     /translation="MSEEASKLPKDIAAAEGKVNVQINGTWHRFPRGTRIADACRSVG
                     VPIPCFCYHPKLAVAGSCRMCMVEQGMPPRLAPGQTPSYDENGYQRIQWMPRPIISCA
                     NTVAENMGIRTDSPLASEARRGVMEFLLTSHPLDCPICDQAGECKLQEYSNDYGQVEG
                     RFEEKKTKKGKNLSIGPRVNLDQERCVLCGRCVRFMRDIMHDEVLTMSQRGTFNAITV
                     YPGRELDSNYSMNTVDLCPVGALTSKDFRFKMRVWFLKETKTIDVDCGTGTNITLWTR
                     EDKVYRITPRQNDAVNSCWMPDSHRLNYKYINAETRIPQPVIRTDAEAPHRPSTWEPA
                     LASAAEAVKRIAPNQLAIIASGRMTNEELYMVRHLAAQIGTDMVDIVPRMGESDGMLI
                     SADRNPNTNGARLVLDIEPGSRLDAIREGVRSGSIKGILSLGEDLISPEAGFTAEDLD
                     KLDYLFMTAHSANETARHADLVLPGVTYAEKFGTMINVTGRIQRLNRAIQPIGYARDD
                     WQIFRDITLLLGGNPALKDFNSALDILTAMSTEYGALNGVSWGSIGDGGQPILETGVT
                     IPVVEREKNQGR"
     gene            complement(9385..10785)
                     /gene="nuoF"
                     /locus_tag="PK077_02850"
     CDS             complement(9385..10785)
                     /gene="nuoF"
                     /locus_tag="PK077_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRN23269.1"
                     /translation="MSITYLPGKEPHPRETRRILKNVNREGWNTSIDCYLADGGYEEL
                     KKALGMEPRAVIDEVKKSGLRGRGGAGFPTGVKWTFIPPNNTKPVYLVCNCDESEPGT
                     FKDRYIVHQDPHQLIEGMVISSYAVGAHQAYIYMREEFPIAAEIMLKALQEARERGFL
                     GSNIQGSGYNLEIHLHRGAGAYICGEETALLNSLEGDRPYPRIKPPYFPAAIGLYGCP
                     TIVNNVESLCQVKHIMAMGGEAYSQEGAKRSPGTRIIGVSGDVKRPGFYEIVSGSMTF
                     GELLYDVCGGPRDGRQFKAVIPGGSSSKVMRTDAEYKVQNPDGTMGKITFFDIPLDLD
                     SIAQHGSMSGSGAVIVMDDSRSMPWAMNNINRFYAHESCGQCTPCREGCPWMMKLSTR
                     ILEGKAAPSDVDLLIEVANQIEGKTVCAFGEAASWPTQAMVSKFKEEFVALTDPFNAC
                     LPHTEEGREQNRFLTR"
     gene            complement(10819..11400)
                     /locus_tag="PK077_02855"
     CDS             complement(10819..11400)
                     /locus_tag="PK077_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRN23270.1"
                     /translation="MSDYAENAIDATIAHQPSPGERFYPAFEVTPELDAAASEYVTHY
                     PEGKQKSAVLPILHEIQKKFGFISGDAIAWVGEKLNISAAHVLGVVTFYPGLRQMCPG
                     KNHIRVCRTLSCAMAGADSLFDAICTRLGIDKNGIDHHHPIGVSPDGLWSVEGVECLA
                     NCGFGPNMMVNDLLYEKVTPEVLEEVIAKYQTA"
     gene            complement(11450..12694)
                     /gene="nuoD"
                     /locus_tag="PK077_02860"
     CDS             complement(11450..12694)
                     /gene="nuoD"
                     /locus_tag="PK077_02860"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HRN23271.1"
                     /translation="MKTSTKTFAIADTAANADYLTNTSEPLGETMTLNVGPSHPATHG
                     VLRLVLELDGEEIISCDPVVGHLHRGMEKIGETIQYNQFVPYTDRFDYLAPLSNNIAY
                     ACAVEKLLGWELPPRGQALRVLALELSRFSSHILGVGVYGMDVGAMTVFLYCYEEREK
                     IHNFYEQLTGARFTSSYTRIGGQTRDVPNEMLKEVLVFCDEAAKTLDETEALLLKNKI
                     FIDRLQGVGVISREKALSWGITGANLRASGIKRDLRKLTPYLGYENYEFDVPVGEHGD
                     CYDRFTVRIEEMRQSLRIIRQVIETMPDGPINMVDTKGTLPEKKKVLTDMESLIRQFM
                     TTTMGVNAPAGQVYFAAENPKGELGFFLDSKGGGLPNRLRMRSPSFCNLSILPELMKG
                     HLVSDVPAILGSFDFVMGECDR"
BASE COUNT         2802 a         3245 c         4191 g         2580 t
ORIGIN      
        1 tttctgaggg cttgtcccag tacatggagc ggatgacctt gaagtaatag taaaacccgg
       61 atgctgcgca gacgatcatg acggggagca tccaatccag atacagaccc gtattgatga
      121 cgtgcacgaa agaaatcatt ttggcgaaaa atccggccgt cagcggcacg cccgccaggg
      181 aggcgaacat gatggtgacc gccagcgcca gccggggatt ggttttgccc agaccgcgga
      241 acgcggaaat ttcctcgctc ccgcgctgga tgcggatcat ggcaagggcg aaaaaggcgc
      301 cgaaggtcat gaccaggtac acagccaggt agaacggggc gttcggagcc aactggccgt
      361 ccacagtacc gataaacagg ggcagaatga agcctgcctg tgcaatggaa gaatacccca
      421 tcatgcgttt caggttcgtc tggggaatgg cgcccaggtt gcccaccagc agggtggcgg
      481 cggccatcag ggctacgacg aattcgacgg gaggcagcac ggcgaatggg gccaggatga
      541 tgccgagcag ggcgaatccg gctaccttgg aggctacgga aaggaaagct gtcaccggag
      601 tgggagctcc ctggtacaca tccggaatcc acagctgcat gggaactgcg ccgattttga
      661 atgctgtacc gagcagcagc atggcgaggg ccaggtacat ggcgggggct gtctggccgg
      721 ccagggcgtg gctgacgatc gtttcattat aaatgaatgt tcccgtaatg ccgaaatacc
      781 aggccaggcc gaaaacgaga aaccccgtgc tgacggcgcc cagaatcagg tacttgatgc
      841 ccgcttccgt ggaacccagg ttccgccgga agtagcctac catgatgaag gaactcaggg
      901 tgagcacttc cagggagacg aacagttcca ctaggttgga tgccttgcac agggaggtga
      961 tgcctacgca ggccagcagg ggcaggatgt agaattcccc cgttccgtcc tgggatttgg
     1021 aatcattgga ggaaaggttg atgacggccc ggaaatctac ggaaagcaac agggtgactg
     1081 ctgtggcgac gagcgccagg atgatgtaga tgttgtcata gagcccttcc atgtagaagg
     1141 gaagcatcgc cagggttccc gccgcgccaa tcagcccgaa aataaatttg ggcgtttttt
     1201 tgcagaaggt ctccgccaga agcaaaagca ttgccaggcc ggccaggatg aattccggaa
     1261 tatacgcttg catgggtaaa agagaatcag gttagactag gattgtattc ggaagggagg
     1321 aaatcaggag aaaaactgga gcaccagcgt ggggaagaag ccgaagaccg ccagggcgat
     1381 gaccaggaag tagttggccg cccgttcgga gggctgcagt cccgtggcgt aggcggaggc
     1441 tttggacaaa tctccctgga aaatattgcg gtaggccctc agcatgtata ccgcgccgat
     1501 gaccagtccc cagaggcaca ggatggtggc gatttgaacg gggccgaagc cgttgctgaa
     1561 accggcgaaa ccggagaaga agaccatgaa ttcccccggg aaattggcga ggccggggag
     1621 gccgatggaa gccatgcccg ctaggccgaa aacgaaagcc atgcgcggca gcctggtgct
     1681 cagtccgccc agagagttga tttccaatgt gcctgtttgc ttttcaatct gcccgcagag
     1741 cagaaagaga agggcgattg tcaggccatg ggccagcatc agcagagcag cccctttcag
     1801 cgcccaggga ttggcttccg cggaaccgga aactaccagg gcggcgaaag ccaggaagat
     1861 atagcccatg tgcatcacgg aagagttgcc cagcagcagg tccagccttt tctggttgac
     1921 ggtcacgtac cccacccaaa tgacattgca gacgagcagg accagcagga tattcgtcca
     1981 gggaaggaat cctgtttcca tcagcggctg gaacatgaag agcccgtaca ggccaaattt
     2041 cttaagcact ccggcatgca tcatcgctac cggagcggga gcggatgcgt atgcgggggc
     2101 tgcccaggaa tggaagggga agagggaaat cagcgtgccg aatccggcga tgagcagggc
     2161 cccgatgagt tccgcgtggg caagttcgcg cccgctggcc atgaagtctt tcaggccgga
     2221 gaaggtgaag ccagcctgcg tgccgatcat cagcagggct gccagcagca ccatggaggc
     2281 aagccccagg taaagcgtgg cgcgccaggc cgtggtgcgg cggtcgccgc gtccatacag
     2341 gccgatcatc acgaaggtgg ggataagagc cagttcatgg aaggcgaaga aggagattat
     2401 gttgtcggac aggaacgccc cggtggcgcc tgcggaaatc agaagggcgg agttgtacca
     2461 ggaagcttcc cccccctggg gcgggttggt gcccaacacg gtggcgaagg taaccaggat
     2521 ggtcagcagc agcattactt tggacagggc cggggccaac gtgagctgga ggtccatccc
     2581 ttcaaagcgg gaccagcagg aagaacagcc gtcaaagcta accagggccc agatgccgag
     2641 ggccagcgtc agggaggcgc tgagcagggc tgtcgggcgt gcgggcgcct tgcacagacc
     2701 gatgagggcc gcggcaatca gaggaatgag aacaagccag ataagcatgg tatgtgattg
     2761 gttggttagg ttagcaggaa tagaagacgg taaagtagat gaccaggagc aggccaatgc
     2821 cgaagagggc cgtataggcg gccatgttgc cggactgcag gcggcgcagc agggagccta
     2881 tggcggcggt caaccgcgcc agtccgccaa cgatgaggcc ggagatgatg aactggtcca
     2941 taaagtcgat gatggcggcc agaacgccct ggactcccct gaccagcact ttatcataga
     3001 acagatcaat ataaaggcgg ttggcgagag cccgggaaac ggcattgccg gagagtttgt
     3061 cagaagccgg ggatcctgcg tagaaggcag ccgccagcag gattccgagc accagcgccc
     3121 ccatggagac gtagaagggc atgcccatgt ggaagctttc ggaaacgaat ccgttgaaag
     3181 gaaccagcct gtctgcgatg aacccgtagc cggagatgag agccaggaca gccagaatca
     3241 gcaggggcac cagcatgagg ccgccaactt ccgaggcgtg ttccgaactg tggctgcggc
     3301 tcttgccgaa gaagaccaca aagatgacac gcatcatata gaaggttgtc agcagggcta
     3361 cgcccgcgcc gatccagaag aagaccgggt ttttgcgaag ggctgcttcc agaatggctt
     3421 ccttggagaa gaagccggac gtgaacggaa tggcaatcag cgccatggtg gcaatgatga
     3481 aggtgaggga agtcagcttc atgcgcttca tgatgccgcc cattttccag atgtcctgtt
     3541 catggtggca ggcgaagatg atggcaccgg cgcccaggaa gagcagagcc ttgaaccatg
     3601 cgtgggtgaa gaggtggaac atggctgcct ccccggccag aagtcccagg gccatgacca
     3661 tatagccgag ctgggagagg gtggagtagg ccaggacgcg tttgatgtca ttctgctggg
     3721 tggccatgaa ggcggcgatg acggccgtaa tggctccgat gccggcaatg acattgcagg
     3781 ccagggcggg gaaggcctca atgccgatgg agtattggac gcgcaccatc atgtaaacgc
     3841 cggcggctat catggtggcc gcatggatca gggcggagac gggggtgggg ccttccatgg
     3901 cgtccggaag ccagacgtgc agcgggaatt gggcggattt gcccaccgct ccgcagaaga
     3961 ggcacagtac cgtaatgttc agcaggtttt cactgattcc tgcgggtatt tccatcaggc
     4021 tgaaatccag cgtgttggtc agcgcccaga gagtcagaat gccgatcagg aagccgaagt
     4081 ccccgacgcg gttgcagatg aaggcttttt tggcggcgtt ggcggcagtg tcccgtgtgt
     4141 accagtggcc gataagcaga taggaggaga ggcccaccaa ttcccagaag ataaaggtca
     4201 tggcgatgtt gttcgccagg acgatgcccg tcatggagaa catgaaaagg ctcaggcatg
     4261 cgaagtagcg tgttttggcc ttgtcgtccg ccatgtaagc cagagagaaa acatggacca
     4321 gcaggccgat gcccgtgacc accagcatca tacgggagga aaggggatcc agcacgaaac
     4381 ccaggtccac ggaaaagatg tcggaaatcg ggagccagga gaaggaatcg gatccggtcc
     4441 ggtccagcag acccagggaa aggacgaagg tcgccaggca ggagaaggtg gaggtaaggg
     4501 ccgcgacgtc aggacgtttg gtcagcagga accagtcaaa ggcggctacg accagcggca
     4561 ggaagagcag aagccaggtg tattgaatat caatattgaa catgttcgtg gagtagtgtg
     4621 ttgctcttag tctttcagcg tgttgagatc ttgcgtgctg gccgtgcgcc tggccctgta
     4681 cagggagacg atgagggcca ggccgatagc cacttccgct gcggcgatgg tcaggataaa
     4741 gatggagagg gcctggccgt catagttggg caggcccata gtgccctggg cgcgggaaaa
     4801 cgctaccagg gacaggttgg cagcactgag catcatttcc aggcacatga agatgacgat
     4861 gatgtcgcgc cggacaatca cccccatcag gccgatggcg aacaggacgc cggagaggat
     4921 cagataatgc gtaagaggta tcatggtgat ggctgtggta aggggttctt tattttccgg
     4981 aagggcggcg gctcagcacg acgacgccga tggaagccac cagcagggcc agaccggcga
     5041 tgaccaggct ccggttgtat ttgtcaaaca gggtacggcc cagcagggcg gtgtccggga
     5101 attctccgtt gtctatctcc tcacggatgg tggtgccttc cggatagagg tgggcggaac
     5161 attgggggga gagggggggg agaatgacgg aaggggcgcc gttgttgcga aggctacagg
     5221 cgggagtttc cattccggtt ttggaggggg ctgcgtcctc ctgcccgatt ttcaaatcgt
     5281 tccaggcctg ttccgcgctg gccatcgggc agcggtgcgt ttctttggcg ccgggcaaag
     5341 aatagatgat gccaataagc tggccgagga agaggcaggc gatgatgaca ccgatagcga
     5401 cggagaaagg cctgctgaag ggggatgttt cctgcttcac attcagcagc atgatgatga
     5461 acgcgaagag caccataatg gccccggcgt atacaaggat ttggagaatg cccaggaagt
     5521 gggctcccag cccgatcatg acggcggcgg tggctccaaa ggaaagagcc atcatcatgg
     5581 cggaggagac ggggttgcgc atgaagacga ccatcaggga caggaccacg gccagggccc
     5641 cgaaaacgta aaaaagtatg tcgctggcaa agatgttcat aacgtacggg aaatggagtg
     5701 ttcgggggtt atttgtattg gttccacttg ttgaccagcc ccttgcgggt gccgcccagt
     5761 tcgtagagct tggccttgtt gtggatggcc tgggttttat ccgtgggggt gaacaggtag
     5821 ttatggctca tgaagatggc ctgttccggg caggcttcct cgcacatgcc gcagtagatg
     5881 cagcgcagca tgtcaatctg aaattccctg ggggcttttt ccactttgcc ccacggtcct
     5941 tcctgtatgg ggccgggggt gatggtgatg gcgcgggccg ggcagatgaa ttcgcagagc
     6001 tggcaggaaa cgcagcgttc acggccgtct tcgcctttca ccagcacggg agctccgcgg
     6061 taatatttcg gcagatgttt gtcccatctg gcttccggat attgcatcgt aacgccgatg
     6121 ccggaaccgg ccagttcttt tttgttgcgg gatttgccca gcagagccag aacgaggtgc
     6181 ctgatagtga gccaaagacc gccaagaatg gattgcagat agaaccgctc tccaagggaa
     6241 atcttcggac gcttgatcgt tttgaaggcc ataatagtaa tgttgtcgga atgaagaagg
     6301 gggggattac ttgacgaagt acaggatggc ggctgtaagg aagatgttga tgacggccag
     6361 ttcaaagaag accatccagc cgagcttcat cacctggtca tagcggaagc gggggagcgt
     6421 ccaccgcacc cagacgaaga agaaggcgaa gaagagaagc ttgagcacat atgcgatgag
     6481 ctggcagatg accgccaccc agttggctac gttttcattg agccagccgt ccaggccgaa
     6541 gccgatggac cagcccccca ggaacagcgt gatgacaagg gaggatccca ccaccatggc
     6601 ggcatattcc cccatgaaga attgggcgaa tttcatggag gagtattccg tatggtagcc
     6661 gcccacgagg tccgtttcac attcggacat gtcaaacggc gtacggttgg cttccgcaaa
     6721 aatggaggtg aggaatatga tgaaactgat agctacgggc accagcagta tccagcgctg
     6781 ccagctcagg ccctcgcccc acacgggaag caggagccag ccgttggcgg cctggtattc
     6841 cacgatgttg gacaggtcca gcgtgctgta aatcatcagc acagggatga tggacagacc
     6901 catgctgatt tcataggaaa tcatctgggc tgaggagcgc acgccgccca ggaaggggaa
     6961 tttggagttg gatgaccatc cggccacgga gagggaggag atggcgaaca tccagagggg
     7021 gcctacgctg aggttggcca ccgccatgtt gacgtttccg taagaggttt gaaggtctcc
     7081 ggcgaaggga accacggccg ccgtcatcag cggtggcgca agcactagga tgggagcgat
     7141 ccagaagtat acgcggcgta cgaagggggg cgtgaaatcc tgtttcagga acagtttgcc
     7201 gccgtccacc atgggctgca cgagaccgaa gatggggatg tcgcggtcca ggcggaaaaa
     7261 acggcacagg ttggcgattt tgccgtcatg gggcaacccg aagtagtcca gcgtcttttt
     7321 gaacggcagg tcggatttca tgccgaagcg gcggaagatg cttaacggaa tgccggcgcg
     7381 gttggggcct acgcggtcct ggatccaggc ggccacccgg cgttcaatgt acacgcagac
     7441 cggaatgaac agaatggtga tggcaaagca gatgacgatt ttgatcagca gagtgatgat
     7501 ataaaaccac acagggttgc tgagcacctc gtcgcaaaag gttagaatcg aatcaatcat
     7561 ggcggattaa caggttgtat gcgtgtgaag tgtgattagc ggccctggtt tttttcgcgt
     7621 tccaccacgg gaatggtgac gccggtttcc agaatgggct ggcccccgtc tccgatggaa
     7681 ccccaggaga ctccattgag tgcgccgtat tccgtgctca tggcggtgag gatgtccagg
     7741 gcggagttga agtctttcag agcaggattg cctcccagca gaagggtgat gtctctgaaa
     7801 atctgccagt cgtcccgggc gtatcctatg ggctggatag cgcggttcag gcgttgaata
     7861 cggcccgtga cattaatcat ggtgccgaat ttttcagcgt aggtgacgcc cggcagaacc
     7921 aggtccgcat ggcgcgccgt ttcattggcg ctgtgggcgg tcatgaacag gtagtccagc
     7981 ttgtccagat cttccgccgt gaagcctgct tccggggaga tgaggtcttc ccccagggag
     8041 aggatgccct tgatggaacc gctgcgaact ccttcgcgaa tggcgtccag cctggaaccg
     8101 ggttcgatat ccagcaccag cctggccccg ttcgtgttcg ggttgcgatc cgcagaaata
     8161 agcatgccgt cgctttctcc catgcgggga acgatgtcca ccatatccgt gccgatttgg
     8221 gcggccagat ggcggaccat gtaaagctct tcattggtca tgcgcccgga ggcaatgatg
     8281 gcgagctggt tcggagcaat gcgcttgacg gcttccgcgg cggaagccag agccggttcc
     8341 caggtggagg ggcggtgggg cgcttccgca tccgtacgga tgacgggctg gggaatgcgg
     8401 gtttccgcat tgatgtattt gtagttaagg cggtgagagt ccggcatcca gcaggagttt
     8461 acggcatcat tctggcgcgg ggtaatgcgg tataccttgt cttctcttgt ccagagggtg
     8521 atattggtgc ccgttccgca gtccacgtca atggtctttg tttccttcag gaaccagacg
     8581 cgcattttaa agcggaaatc cttggaagta agggctccta ccgggcagag gtccacggtg
     8641 ttcatggagt agttgctgtc cagctcgcgg ccggggtaaa ccgtgatggc gttgaaggtg
     8701 ccgcgttggg acatggtgag cacctcatcg tgcatgatgt cgcgcatgaa gcgcacgcag
     8761 cggccgcaga gcacgcagcg ttcctggtcc aggttcacgc gcggaccaat gctcaggttt
     8821 tttccttttt tggtcttttt ctcttcaaaa cggccttcca cctggccgta gtcgttggag
     8881 tattcctgaa gcttgcattc cccggcctga tcgcagatgg ggcagtccag agggtggctg
     8941 gtgagcagga attccattac gccgcggcgc gcttcactgg ccagggggct gtccgtgcgg
     9001 atgcccatgt tttccgccac cgtgtttgcg caggagatga tggggcgggg catccactgg
     9061 atgcgctgat agccgttttc atcataggag ggtgtctgtc ccggagcgag cctgggcggc
     9121 atcccctgtt ccaccatgca catgcggcag gagccggcga cagccagctt ggggtggtag
     9181 cagaagcacg gaatgggcac gcccacggaa cggcaggcgt ctgcaatccg cgtgccgcgc
     9241 gggaaccggt gccacgttcc gttgatttgg acgttcacct tgccttccgc ggcggcaatg
     9301 tctttgggga gtttggaagc ttcttcactc atgatgacgg atgggagagg gatggttttg
     9361 agaactggtg ttgcgtgctg ctgtttagcg ggtgaggaaa cggttttgct ccctgccttc
     9421 ctccgtgtgg ggcagacagg cgttgaatgg atccgtcagg gctacgaatt cctccttgaa
     9481 tttggagacc atggcctggg tgggccagga ggcggcttca ccaaaggcgc agaccgtttt
     9541 tccttcaatc tggttggcca cttcaatgag cagatccaca tccgacgggg cggcttttcc
     9601 ttccaggatg cgggtgctga gtttcatcat ccacggacag ccttcccggc agggagtgca
     9661 ctgcccgcag gattcatggg cgtagaaacg gttgatgttg ttcatggccc agggcatgga
     9721 gcgggagtcg tccatgacga tgaccgcgcc ggaaccggac atggagccgt gctgggcgat
     9781 gctgtccaga tccagcggaa tgtcgaagaa ggtgattttt cccatggtgc cgtccggatt
     9841 ctgcactttg tattccgcat ccgtgcgcat gaccttggag gaggagcctc cgggtatcac
     9901 ggccttgaac tggcgcccgt cccgcgggcc gccgcagacg tcatacagca gttcaccgaa
     9961 ggtcatggag ccggagacga tttcatagaa cccaggacgt ttgacgtcgc cggaaacccc
    10021 gatgatgcgg gtgccggggg aacgtttggc tccttcctgg ctgtaggctt ctcctcccat
    10081 ggccatgatg tgtttcacct ggcagaggga ttccacgttg ttgacgatgg tggggcatcc
    10141 gtacaggccg atggcggccg ggaagtaggg gggcttgatg cggggatagg ggcgatcccc
    10201 ttcaagggag ttgagcaggg ccgtttcctc accgcaaatg tacgctcccg ctccgcggtg
    10261 caggtggatt tccagattat agccggaccc ctggatattg ctgcccagga agccgcgttc
    10321 gcgagcttcc tgaagggctt tgagcatgat ttccgcggcg atgggaaatt cttcgcgcat
    10381 gtagatataa gcctgatgtg cccccactgc gtagctggag atgaccattc cttcaatgag
    10441 ctggtgtgga tcctgatgga caatgtagcg gtccttgaat gtgcctggtt cagattcgtc
    10501 acagttgcaa accaggtaaa caggcttcgt gttgttcggc ggaatgaagg tccatttcac
    10561 gccggtgggg aaccccgcgc cgccacggcc gcgaagaccg gattttttca cctcgtcaat
    10621 gacggccctg ggctccatgc cgagcgcctt tttcagttct tcataaccgc cgtcggccag
    10681 atagcagtca atggaggtat tccagccctc gcggttgacg tttttcagaa tgcggcgcgt
    10741 ttccctgggg tggggttctt taccgggaag ataggtgatg ctcatggcga aaatctggtg
    10801 cgtgggaaat ggaaagggtt aggctgtctg atatttggca atcacttctt ccagcacttc
    10861 cggagtgact ttttcgtaca ggagatcgtt gaccatcatg ttggggccaa agccgcagtt
    10921 ggccaggcat tccactcctt ccacgctcca caggccgtcc gggctgactc caatggggtg
    10981 gtggtggtcg atgccgtttt tgtcaatgcc cagacgcgtg cagatggcgt cgaacaggct
    11041 gtccgccccc gccatggcgc aggaaagagt gcggcatacg cggatgtggt tttttccagg
    11101 gcacatctgg cgcagaccgg gatagaaagt caccacgccc agaacatggg cggcggagat
    11161 attcagtttt tctcccaccc aggcaatggc gtcgccgctg atgaagccga attttttctg
    11221 tatttcatgc aggatgggca acacggcgga tttttgcttg ccttccgggt aatgcgtaac
    11281 gtattcgctg gcggcggcgt caagttccgg cgttacttca aaggccgggt aaaaccgttc
    11341 gcccgggctg ggctggtggg cgatagtcgc gtcgatggcg ttttcagcgt aatcggacat
    11401 ggggagaaag tttggtgttt gcgttgaagt ggatgaaagt ggcgggtgtt taacggtcgc
    11461 attcgcccat cacgaagtcg aaggagccga gaatggccgg aacgtcggaa accaggtggc
    11521 ctttcatcag ttccggcagg atggacaggt tgcagaagga gggggagcgc atgcgcaggc
    11581 ggttgggaag cccgcccccc ttggagtcca ggaagaaacc cagctctccc ttcgggtttt
    11641 ccgcggcaaa gtaaacctgc ccggcagggg cgttcacccc catggtcgtc gtcatgaact
    11701 ggcgaatcag ggattccatg tccgtcagga cttttttctt ttcaggcagc gtgcctttgg
    11761 tgtccaccat gttgatgggg ccgtccggca tggtttcaat gacttggcgg atgatgcgca
    11821 gggactggcg catttcttca atgcgcacgg taaaacggtc gtagcagtct ccgtgttcgc
    11881 ctacggggac gtcgaattcg taattttcat atcccagata gggggtgagt ttgcgcaggt
    11941 cccgcttaat gccgctggct cgcaggttgg cccccgtaat accccaggaa agcgcttttt
    12001 cacggctgat gacgcctacg ccctgaagac ggtcaatgaa gattttgttt ttgagcagga
    12061 gggcttccgt ttcatcaagg gttttggccg cttcatcgca gaagacgagc acttccttga
    12121 gcatttcatt gggaacatca cgtgtctgtc cgccgatgcg ggtataggag gaagtgaagc
    12181 gcgccccagt gagctgttcg taaaaattat ggattttttc acgttcctca taacagtaca
    12241 ggaaaacggt catggcgcct acgtccatgc cgtacacgcc tacgcccagg atgtgggagg
    12301 aaaagcggga aagttccagg gccagtacgc gcagggcctg tccgcggggc ggtagttccc
    12361 atcccagcag tttttccacc gcgcaggcat aggcgatgtt gttggacagg ggggccaggt
    12421 agtcaaagcg gtcggtgtag ggaacaaatt ggttgtactg gatggtttcg cctatttttt
    12481 ccattccgcg gtgcagatgg ccaaccaccg gatcacagct gatgatttct tcaccatcaa
    12541 gttccagcac taggcgcagc acgccgtggg tggcggggtg ggaggggccc acattcaggg
    12601 tcatcgtttc tccgagcggt tcggatgtat tggtgagata gtctgcgtta gcggctgtat
    12661 ctgcaatggc gaaagttttg gtgctcgtct tcatgtggat gtccgtgcct ggctgaacct
    12721 gataatggtg ccggatgatt atgccacttc acaccatgcg ggcaagccaa atgtcagttt
    12781 ttttggcatt ccggggaggt ggtgcgggag tctcctca
//
LOCUS       DAOMSU010000036        12785 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_237_length_12785_cov_3.232679, whole genome shotgun sequence.
ACCESSION   DAOMSU010000036 DAOMSU010000000
VERSION     DAOMSU010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12785
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_237_length_12785_cov_3.232679"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1072)
                     /locus_tag="PK077_02865"
     CDS             complement(98..1072)
                     /locus_tag="PK077_02865"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRN23272.1"
                     /translation="MAVDFGGTSIKMGVTAGERILATADRIPTAMFESPQAIIDAMIA
                     SARTLRGQFPSACVMGMGMPGWCDYQRGVLYQLTNVRVWDREIPVKEMMEQALGLPVV
                     LDNDANCMAYAEWKMGAGRGMSSLVCLTMGTGIGGGIVVHDRMLRGRRLSAAELGQTS
                     IHYQGKTGPFGSRGAIEEYIGNNELAAEAVKRYAGAGIIKTVDECTPRHLDEAARSGC
                     PIALQLWEDTAEMLGCLIMNLMYTLVPDAFIIGGGVAKAGDLLMKPLLENLRKQLFPL
                     LMEDLKILPARFGAEAGLLGAGAMAMDEFMGMGILERFKNQKSTQTFC"
     gene            1241..1786
                     /gene="tadA"
                     /locus_tag="PK077_02870"
     CDS             1241..1786
                     /gene="tadA"
                     /locus_tag="PK077_02870"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HRN23273.1"
                     /translation="MITSVMMEMPGSDEWFMRQAMKESRKALVKGEVPVGAIVVKDGR
                     VIGRGWNQVETLKDATAHAEMIALTAAQEALGDWRLEGCTLYVTKEPCPMCAGAIVHC
                     RPDRVVFGCPDAKTGAAGGWINLLDSNPPLNHKCEVRPGVLGDECLLHLQEFFRAARL
                     AAKERKKLSARLSSPPDENGE"
     gene            1849..2787
                     /gene="lipA"
                     /locus_tag="PK077_02875"
     CDS             1849..2787
                     /gene="lipA"
                     /locus_tag="PK077_02875"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRN23274.1"
                     /translation="MERRNDNETAEGTERKPSWIKVRLPNGREFWDVKQLVEGKNLFT
                     VCEEAHCPNRYECWNQGTATFMIAGERCTRACGFCATRTAKPGPLDPEESQHVAEAVV
                     HMKLRHAVITMVTRDDLPDGAAAHFAQVIRAVRKASPSTIIEVLASDLNEKPSSIQTL
                     MAARPHIFGHNLETVERLTPIVRFRAQYRRSLRVLRMALECVDGKVSTKSGIMLGLGE
                     TEEELFRSMDDLLEHGVTVLTLGQYLRPSRNHLPVVKYVHPDDFARYEEVARSKGFRH
                     VASGPLVRSSYHAANFTPEADVLEAINEDLRKAGEL"
     assembly_gap    2989..3000
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3001..4238
                     /locus_tag="PK077_02880"
     CDS             <3001..4238
                     /locus_tag="PK077_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HRN23275.1"
                     /translation="TMNSKCADCHKPGTHISELVNTLSGGLLARHIRDGQRSYNMEEP
                     PTAVTLSKLEHVLQINSMPPTSYTMVHWGSTLTLREKNAMLQWIKDERLKIFGDMVGE
                     EYALSPLAPIPDALPTDPAKVALGYKLFHDVRLSTDNTVSCASCHSLEKAGTDNLPTS
                     TGVHSQKGGINAPTVFNAAFHAKQFWDGRAANLQEQAGGPPLNPVEMGYEHPDDWKKI
                     AAKLDQDTAFAVEFKKVYPQGFTGETITNAIAEYEKTLITPNSPFDRYLKGDENAISE
                     NAKKGYKLFLKLGCQTCHTGPAMGGQSFEYADLKGDFFAGRAKTNDDNGLMNFSKKES
                     DRHRFRVPTLRNVELTWPYMHDASAQTLEEAITKMYHYQLGYDKLDKKEVRLLVAFLK
                     TLTGEYNGKPVQGEVCPAS"
     gene            4489..4719
                     /locus_tag="PK077_02885"
     CDS             4489..4719
                     /locus_tag="PK077_02885"
                     /inference="COORDINATES: protein motif:HMM:NF015956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HRN23276.1"
                     /translation="MIHTRTMPLSMLNIGDIRQVNKIHGKDETRRFLESLGFVAGSTV
                     SIVSENGGNFIINVKGSRIALSKALASKIFVA"
     gene            4751..4981
                     /locus_tag="PK077_02890"
     CDS             4751..4981
                     /locus_tag="PK077_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HRN23277.1"
                     /translation="MTKHRTLRDALIGETVTVGKLAGEGPVRRRIMDMGVTKGTQIFI
                     RKVAPLGDPIEVTVRGYELSIRKSEAENIHIH"
     gene            5058..7319
                     /gene="feoB"
                     /locus_tag="PK077_02895"
     CDS             5058..7319
                     /gene="feoB"
                     /locus_tag="PK077_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HRN23278.1"
                     /translation="MSNITIALAGNPNCGKTTLFNALTGANQYVGNWPGVTVEKKEGR
                     LKGHKNIIIEDLPGIYSLSPYTLEEVVSRNYLVTDHPSLILNLVDGSNLERNLYLTTQ
                     LCELGVPVIIALNMMDIVRKRGDRIDTEKLSRNLGCQVLEISALKGTGIRELIDAVVT
                     TTASDDARTPQAVHFPDHLEKAIGEIGEIITGKCLDQQKKWFAVKVFERDQKVADQLA
                     LNPEEQSRINGIIETMEREMDDDSESIITNERYNSISRIIKDAVVKASPGSLTGSDRI
                     DRIITNRWLGLPIFAVVMWAVYYISIQTIGAWGTDWANDTFFGEWVPEWVGNLLENAG
                     CAPWLESLIQDGVIAGVGAVLGFLPQMAVLFLCLGILEDCGYMARVAFIMDRIFRKFG
                     LSGKSFIPMLVSMGCGVPGVMATRTIENEKDRRMTIMTTTFIPCGAKTPIIALIAGAF
                     FPENSWVAPGAYFIGVGAIILSGLILKKTAMFAGDPTPFVMELPIYHIPAWKNVIIHA
                     WDRCKAFVQKAGTVIFVSSALIWFLSSYNWKVDSVEQNDSMLASIGNVAAPVFQPLGW
                     GEWKPTMATITGLIAKENVVGTFGVLYADGSSGEDEAEKTEDSPETQEMAPAGRELQA
                     SLEEGSGESAAEAEEEEDEDEEIRETGDRLVQAGAFTFLSAFSFMIFNLLCAPCFAAM
                     GAIRREMNSAKWTFFAIGYMCALAYCLAFLIYQLGAWLYAEASFGIGQGIALLLLLGL
                     VYLVAKKPYTASK"
     gene            7354..7539
                     /locus_tag="PK077_02900"
     CDS             7354..7539
                     /locus_tag="PK077_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23279.1"
                     /translation="MGTYIIATLLFLALAYALYKIFSKRGKCCGNCGCCGNDSDGCCG
                     GGGNCCGGHHHDHEKQD"
     gene            complement(7638..8045)
                     /locus_tag="PK077_02905"
     CDS             complement(7638..8045)
                     /locus_tag="PK077_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23280.1"
                     /translation="MSKVQWEISNDEVTDRDTALRRLQEGTTGHVVCARDGACLQSYK
                     EADGVYHCEIVFKTDSCCPPVFAAPEGVTGKELEMLYDRFANGDDFSFVEKEWEPLMA
                     VEYRHRHNVMWFIFLFIALVIAAMAAYQHGWIE"
     gene            complement(8592..9767)
                     /locus_tag="PK077_02910"
     CDS             complement(8592..9767)
                     /locus_tag="PK077_02910"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRN23281.1"
                     /translation="MALPVIAQDSTPAVGDILQGINMFTPEEGDPAYKVKANEKYGTQ
                     WGVDMAYGLWNTEKVSDVKRHNNLALLHAQLNQRLIEDKANGGTWLRVEFSGSWGLDR
                     ESAQSDTFFTEAYATASGLHADAMGPHEGIFPEVALMQYFAGKRACLIAGMVNLTNYF
                     DAVSIANDSFSSFTNDGFVNSTILPLVDSNIGGILQVELNRNNYMMVAVSRTGCDSGY
                     NPFNSDYCDGYAVVGEYGHIFADGAATLRINPFYTSTDVDMDDGTGERRRQNAGLVAS
                     IEYTPCDPLTIYSRAGFAAKQYLSNSAEFSVGANIKLFPSREDDFLGISYGVFKGQTP
                     CDGERAEHNREQVLEVMYSFQVNDYFKVVPHFQYIANPAYSTSSENILWGVQAVFSF"
     gene            10392..10613
                     /locus_tag="PK077_02915"
     CDS             10392..10613
                     /locus_tag="PK077_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23282.1"
                     /translation="MNNKGLWGFVGGMIAAAIGAKVVKSPVARKLAVQGLSKAMLIQQ
                     KAMEQLANIREEAQDLCHEAAAKNSRPEQ"
     gene            10671..>12785
                     /locus_tag="PK077_02920"
     CDS             10671..>12785
                     /locus_tag="PK077_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRN23283.1"
                     /translation="MDRPQRGKRTEKGVSPFSFSLSMHYQVIHHTPGRLRVRSGRCAF
                     NHDQAYGIECRLLKQKGVYSVKATPCNGGVFILYEGTSPQAIFRTLDRLRPETLRSHE
                     PEERAEARKLARSFFLRIAGKASSFLFCKVFLPPPLRMAKVFWNYSSYLRRGMAGLGS
                     GRLNVAVLDAVSIGVSIGTKAYGTANSIMFLLSISDLLENYTRKKTRAALAASLSINI
                     DRVWRVEEGGMCQVPMEQIRPGEKIRVDAGNVIPVDGTVLSGEAEVNQAAMTGESEAA
                     SKREGSVVFAGTTLETGSLVIRVDAAGDQSRINNIIALIDHSEELKARIQSRAEKLAD
                     SIVPYTLLTAGALFLFTRNISKALSVLMVDYSCAIKLATPISVISAMKEAAARKIMIK
                     GGKFMELFAKTDTIVFDKTGTLTSACPQVTQIIPLSDCSREYILKTAACLEEHFPHSV
                     ARAVVRKALEEGLHHEEEHAEVEYIVAHGISSRLHGKKVLVGSYHFLFEDERIPLTEE
                     QRQTIRNHARGKSNIFLAIGRRAIGMIGVSDPPRPEAAETIARLKRQGISSIIMLTGD
                     SESAARAISRQLGITEYRSQVLPEDKARFIQQLKKSGKTVCMVGDGINDSPALSCADV
                     SVSMKDSSDIAREVADISLLSSSLAELVVLRELSCAVLEKIERNYRFIVGFNSSLILL
                     GMFGLITPAFSAFLHNASTVYVS"
BASE COUNT         3042 a         3730 c         3272 g         2729 t
ORIGIN      
        1 accttccgga aaatgttcgt gggaatgttc cgtgctcatg acctgttcct cttgtacgcc
       61 tgcctctccg gcatggcaag ccctatatta gcccaaacta acaaaaagtc tgcgttgatt
      121 tctgattctt aaaccgttcc aaaatcccca tccccatgaa ttcatccatc gccatggctc
      181 ccgctcccag caaccccgcc tccgctccaa atctggcagg cagaattttc aaatcctcca
      241 tcaggagagg aaacaactgt ttcctgaggt tctccagcag tggcttcatc aacaaatccc
      301 ctgccttggc cacaccgccc ccgatgatga aggcgtccgg caccagcgta tacatcaggt
      361 tcatgatcag gcagcccagc atttccgccg tatcttccca taattgaagg gctataggac
      421 atccggaccg ggcagcctcg tccagatgcc tgggcgtgca ttcatccacc gtcttgatga
      481 ttcccgcccc ggcataccgt ttaaccgcct ccgccgccag ttcgttgttg ccgatgtatt
      541 cctcaatggc tccccggctg ccgaacggtc ccgttttccc ctggtaatga atgctggtct
      601 ggcccagttc cgcagcggaa agccgccttc cccgcagcat gcggtcatgc accacgattc
      661 ccccgcctat ccccgttccc atcgtcaggc acaccaggct ggacatgccg cgcccggcgc
      721 ccatcttcca ttccgcataa gccatgcagt tggcgtcatt atccagcacg acggggaggc
      781 ccagggcctg ctccatcatc tctttcaccg gaatttccct atcccagacc ctcacattgg
      841 taagctggta aagcactccc cgctggtaat cacaccatcc cggcattccc atgcccatca
      901 cacaggcgga ggggaattgt ccgcgcaggg tgcgggcgga cgcaatcatg gcatcaatga
      961 ttgcctgcgg gctttcgaac atggcagtag ggatgcggtc ggccgtcgcc agaatacgct
     1021 cccccgccgt tacgcccatt ttgatgctcg tgccgccgaa atcaacggcc aaggcacagg
     1081 gttctgaaaa gctcatagtc catacttatg ccacccattc tccggaatca acgtaaaaac
     1141 gcattcatcc ccaccccttt ccgcggcatg gcgcacttat cgtataaaac acaataagaa
     1201 tcttccagaa agagcttgcc agaaccgcgc cgcccggtcc atgataacct ccgtaatgat
     1261 ggaaatgccc ggttcggacg aatggttcat gcgccaagcc atgaaagaat ccagaaaggc
     1321 cctcgtgaaa ggggaagtgc ctgttggagc cattgtggtg aaggatggcc gcgtgatcgg
     1381 ccggggctgg aaccaggtgg agacgctcaa ggatgctacg gcccatgcgg agatgattgc
     1441 cctgacggcg gcccaggagg ctttgggcga ctggcggctg gaaggctgca ccctgtatgt
     1501 caccaaggag ccgtgcccca tgtgcgcagg agctatcgtc cactgccgtc cggaccgcgt
     1561 cgttttcggc tgcccggatg caaaaaccgg ggctgcgggg ggctggatca atctgctgga
     1621 ttccaatccg cccctgaacc ataagtgcga ggtgcgtccc ggcgtactgg gagacgagtg
     1681 cctgcttcat ctccaggagt ttttccgtgc ggcgcgtctg gcggcaaaag aacggaaaaa
     1741 attgtctgcg cgtctttctt cccctccgga tgaaaacggg gaatgaagtc cggaataaaa
     1801 cactttgcat ttgccaaagc agcagagtca cggcaagcta gcgggactat ggaacgccgg
     1861 aacgacaatg agactgcgga aggaacggaa cgcaaaccgt cgtggatcaa ggttcgtctg
     1921 cccaacggcc gggaattctg ggacgtcaag cagcttgtgg aaggcaaaaa cctcttcacc
     1981 gtctgtgaag aagcacactg ccccaaccgt tatgaatgtt ggaaccaggg tacggccacg
     2041 ttcatgattg ccggagaacg gtgcacgcgc gcctgcggat tctgcgccac ccgcacggcc
     2101 aagcccggcc cgctggatcc tgaagaatcg cagcacgtgg cggaagccgt cgtgcacatg
     2161 aagctgcgcc acgccgtaat taccatggtc acccgggatg atttgccgga cggcgccgcc
     2221 gcgcatttcg cccaggtaat tagggccgtc agaaaagcca gcccttccac gatcattgaa
     2281 gtgcttgcct cggacttgaa tgagaagccc tcctccatcc agacgctgat ggcggcccgt
     2341 ccgcatattt tcggacacaa tctggagacg gtggaacgcc tcacgcccat tgtccgcttc
     2401 cgcgcccagt accggcggtc cctgcgcgtt ctccggatgg ctctggagtg cgtggacggc
     2461 aaagtgtcta ccaagagcgg catcatgctg ggcctggggg aaacggaaga agagcttttc
     2521 cgctccatgg acgatttgct ggagcacggc gtcacggtcc tgacgttggg gcaatacctg
     2581 cgcccctccc gcaaccatct tccggtggtc aaatacgtcc atccggatga tttcgcccgg
     2641 tatgaggaag ttgcgcgctc caaaggcttc aggcacgtgg catccgggcc gttggtccgc
     2701 agctcctacc acgccgccaa tttcaccccg gaagcggatg ttctggaagc cattaacgaa
     2761 gacctgagaa aggctggcga actttagaat aaatcttgat tttttatccc tcccctttta
     2821 ccatccgggc aattccacgt cattgaaaat ctcatgagca aaacatgttc cttcatcaag
     2881 gcaggagcca ttctgggcgg cacggtcatc gtgacggcgg ctgtcgcccc cctgttcctg
     2941 ccgaaccaga atgtcaagcc actgacgtcc gcccaggcgg cggaaattnn nnnnnnnnnn
     3001 agaccatgaa ttccaaatgt gcggactgcc acaagcccgg cactcacatt tccgaactgg
     3061 tcaatactct ttccggaggc ctgctggcgc gccatatcag ggacgggcag cgcagctaca
     3121 atatggaaga acctcccact gccgttaccc tttccaagct ggaacatgtg cttcaaatca
     3181 attccatgcc ccccacttcc tacaccatgg tgcactgggg cagcacgctc actctccggg
     3241 agaaaaacgc catgctccag tggatcaagg atgagcgcct gaaaattttc ggcgatatgg
     3301 taggagagga atacgccctt tcccctcttg cccccattcc ggacgccctc cccacggatc
     3361 cggccaaagt tgccctgggc tacaagcttt ttcatgacgt gcgcctttcc acggacaata
     3421 ccgtctcctg cgcttcctgt cattccctgg aaaaagccgg gacggacaac ctgcccactt
     3481 ccactggagt ccacagccag aaaggcggca tcaatgcccc caccgttttc aatgccgctt
     3541 tccatgccaa gcaattttgg gacggacgcg cagccaacct ccaggaacag gccggcggac
     3601 cacccctgaa tccggtggaa atggggtacg aacatccgga tgactggaag aagatcgctg
     3661 ccaaactgga ccaagacacc gcttttgccg tagaattcaa aaaggtttac ccccagggat
     3721 tcaccggaga gaccatcacg aatgccatcg cggaatatga aaaaactctt atcacgccga
     3781 acagcccgtt tgaccgctac ctgaaagggg atgaaaacgc catcagcgag aacgccaaaa
     3841 aaggttacaa gcttttcctg aagcttggtt gccagacctg ccacaccggt cccgccatgg
     3901 gaggccagtc ctttgaatac gccgacctca aaggcgattt ctttgccgga cgcgccaaga
     3961 ccaatgacga taacggcctg atgaatttct ccaaaaagga atcggacagg caccgcttcc
     4021 gcgttccgac cctccgcaac gtggaactca cctggccgta tatgcatgac gcctccgcac
     4081 agacgctgga ggaagccatt acgaaaatgt accattacca gctcggttac gataaactgg
     4141 acaagaagga agtgagactg ctggtggctt tcctgaaaac gcttaccgga gaatacaacg
     4201 gcaaacccgt ccagggcgaa gtttgccctg cctcctgaat ctgaagaaaa gctctttttc
     4261 cagccgcgcc aatgaacatt cattggcgcg gcttttttgc atttcccttc cagttcctac
     4321 ggcaggcact gccgggaaac ttccattccc cgcctcttcc ccgctggcca tacttagatt
     4381 aaactaatac gcaaacaatt tatattaaac gcattttaca ttgattgaaa atatatccta
     4441 ataatatcat tgacttatca gtttgataaa actaacttct ttccggattt gattcataca
     4501 cgcaccatgc cactctccat gctcaacatc ggcgatattc gccaagtcaa caaaatccat
     4561 ggcaaggatg aaacccggcg ttttctggaa agcctgggct tcgttgccgg gagcacggtc
     4621 tccatcgttt ccgaaaacgg cggtaatttc attatcaacg tcaaaggctc caggattgcg
     4681 ctcagcaagg cgcttgccag caaaatattc gttgcctgag ttattccatc caacaaccaa
     4741 ccattattct atgaccaaac atcgtacatt gagagatgcc ctcatcgggg aaaccgtcac
     4801 cgtcggcaaa ctggcggggg aaggcccggt cagaagacgc atcatggaca tgggggttac
     4861 caagggaacg cagatattca tccgcaaagt cgcccctctg ggagatccca ttgaagttac
     4921 tgtccgcggg tacgaactct ccattcgcaa atcggaagcg gagaacatcc atatccattg
     4981 attccgcctc tattaccgca cgtttcctga ctttacagca agacgggcac ccatatatca
     5041 agacaaggaa accattcatg agcaacatta ccatcgccct ggcgggcaac cccaactgcg
     5101 ggaaaaccac gctctttaac gccctgacag gcgccaacca atacgtcggg aactggcccg
     5161 gcgtcaccgt ggaaaaaaag gaaggccgcc tgaaaggcca taaaaatatc atcattgaag
     5221 accttccggg catttattcc ctttccccct ataccctgga ggaagtcgtc agccggaatt
     5281 acctggtcac ggaccatccc tccctcatcc tgaacctggt tgacggcagc aacctggaac
     5341 gcaatcttta cctcaccacc cagctttgcg aactgggcgt tcccgtcatt atagcgctga
     5401 acatgatgga cattgtccgg aagcgcggcg acaggatcga cacggaaaaa ctttcacgca
     5461 acctcggttg ccaggtgctg gaaatcagcg ctctcaaggg gaccggcatc cgtgaactga
     5521 tagacgccgt agtaacgacg actgcgtcgg acgacgcccg gaccccgcag gccgtccact
     5581 ttccggacca tctggaaaaa gccatcggag aaattgggga aatcatcacc ggaaaatgcc
     5641 tggatcagca gaaaaaatgg tttgccgtta aagttttcga aagagaccag aaagtcgcgg
     5701 accagctcgc gctcaatccc gaggaacaat cccgcatcaa cgggattatt gaaaccatgg
     5761 aacgggaaat ggatgacgac agtgaatcca ttatcaccaa tgaacgctat aattccattt
     5821 cccgcatcat caaagacgcc gtcgttaaag ccagccccgg cagcctgacg ggcagcgaca
     5881 ggattgatcg catcatcacc aaccgctggc tgggcctccc catttttgcc gtcgtcatgt
     5941 gggctgtata ttacatctcc attcagacga taggcgcatg gggcacggac tgggccaatg
     6001 atactttctt cggggaatgg gttccggaat gggtaggaaa cctgctggaa aacgccggct
     6061 gcgccccatg gctggaaagc ctgatccagg acggcgtgat tgccggcgta ggagccgttc
     6121 tgggattcct cccgcaaatg gcggttctgt tcctgtgcct gggcatattg gaggattgcg
     6181 gctacatggc ccgcgtagcg ttcatcatgg accgcatttt ccgcaaattc ggcctttccg
     6241 ggaaatcctt tattcccatg ctcgtttcca tggggtgcgg cgttcctgga gtcatggcga
     6301 cccgcaccat tgagaacgaa aaggacaggc gcatgactat catgaccacc acgttcatcc
     6361 cctgcggggc caaaactccc atcattgccc tgattgccgg agccttcttc ccggaaaatt
     6421 cctgggtggc tccgggggcc tatttcatcg gggtgggcgc catcatcctt tccggcctta
     6481 ttctgaaaaa gactgccatg ttcgccgggg atcccactcc cttcgtcatg gaacttccca
     6541 tttaccacat cccggcctgg aagaacgtca tcattcatgc atgggaccgc tgcaaggctt
     6601 tcgtgcaaaa agccgggacc gtcatctttg tgtccagcgc cctgatctgg ttcctctcca
     6661 gctacaactg gaaggtggat tccgtagagc agaatgacag catgctggcc tccatcggca
     6721 atgtggccgc gcctgtcttc cagcctctgg gctggggtga atggaaaccc accatggcta
     6781 ccattaccgg attgattgcc aaggaaaacg tggtaggcac cttcggcgtc ctgtatgcgg
     6841 acggcagttc cggggaagat gaagcggaaa aaacggagga ctcccccgaa acgcaggaaa
     6901 tggcccccgc cggccgggag ctccaggcat ccctggagga aggttccgga gaatccgctg
     6961 ctgaagcgga agaagaggaa gatgaagatg aggaaatcag ggaaaccggg gaccgcctgg
     7021 tccaagcggg agccttcacc ttcctgagcg ctttctcctt catgattttc aatcttctct
     7081 gcgccccctg cttcgcggcc atgggcgcta tcagaaggga aatgaacagc gccaaatgga
     7141 ccttcttcgc catcggctac atgtgcgcct tggcgtactg cctggccttc ctgatttacc
     7201 agctgggagc atggctttat gcggaagctt ccttcggcat cgggcagggc attgccctat
     7261 tgctcctgct ggggctcgtt tacctggtgg caaagaaacc ttatacagcc tccaaataac
     7321 ttctaaacca accccttaac tctactgaac aatatgggaa catatatcat cgccaccctt
     7381 cttttcctgg ccctagccta cgcgttatac aaaattttca gcaagcgcgg caagtgctgc
     7441 ggcaactgcg gatgttgcgg caacgacagc gacggctgtt gcggtggcgg cggaaattgc
     7501 tgcggcggcc accatcacga ccacgaaaaa caggattaaa tcccggcccc ggttatccca
     7561 ttcagaacct gcaaaccatt ttctgcggtt tgcaggttct tctgctacgg cgcctgccgc
     7621 agccagcctc taccctgtca ctcaatccaa ccgtgctgat atgccgccat ggccgcaatt
     7681 accagcgcaa tgaacaggaa gatgaaccac atcacattgt ggcgatggcg gtattccacg
     7741 gccatcagag gttcccattc cttttccaca aaggaaaaat cgtccccatt ggcaaaacga
     7801 tcgtaaagca tttccagctc ctttcccgtc accccttccg gggcggcgaa taccggcggg
     7861 cagcaggaat ccgtcttgaa tacaatttcg caatggtaca cgccatccgc ttccttataa
     7921 gactggaggc acgcgccgtc gcgggcgcac accacgtggc cggtcgttcc ttcctgcaag
     7981 cggcgcagag cagtatcacg gtcggtcact tcatcatttg aaatttccca ttgaacctta
     8041 ctcattctgt cagtttaaga taattcccgt ttaagtcaat atggttttaa acgcttcggc
     8101 ccccggaaac gttgcggctc ccatccatct tccggcgtgg caaaaagcct tcttttccgt
     8161 tttttccggg aacggagaca ggggtgggaa agcccatttt caggcggctt ttatttttcc
     8221 ctgggataaa aattctacgg aatagccttg gggaagcctc ctaccttcaa cagtcagtat
     8281 ttgacggtag ataaggaaag gctgaaggcc attccatgac ccggcataca ttgccggtcc
     8341 aagctccgcc agcaggcatc aataaaggag ggtaaatggc tccgtaatca aataagcaac
     8401 gccgcatcct ctacctgatc gagaatgcgg cgttgccgcc aggtcatgca gaccccttct
     8461 atatctttct cacgcaggat tccgctcact gcatggagac agacaaggaa gattttatgt
     8521 tattatactt tcatgaaact ccggccatgc ctgatttaag agaaaaggcg gccatgtcca
     8581 ggatcagctg atcagaaaga aaagactgcc tgaacgcccc agagaatgtt ttcgctggaa
     8641 gtgctgtaag ccggattcgc gatgtactgg aagtgaggaa caactttgaa ataatcattc
     8701 acctggaagc tgtacatgac ttccagcacc tgttcgcggt tatgctcagc gcgctctccg
     8761 tcacaggggg tctgcccctt gaacacaccg taggaaatgc ccaggaagtc atcttcacgg
     8821 gaagggaaga gcttaatgtt ggcgcccacg gagaattcag cggagttgct caagtattgt
     8881 ttggcggcaa atccggcgcg agagtaaatg gtcagaggat cgcagggagt atattcgatg
     8941 ctcgcgacaa gcccggcatt ttggcggcgg cgttccccgg tcccgtcgtc catgtccaca
     9001 tccgtgctgg tatagaacgg attgatgcgg agagtcgcag cgccgtcggc aaagatatgg
     9061 ccgtattcgc caaccacggc ataaccatcg caataatcgg aattaaaagg attgtatccg
     9121 gaatcgcatc ccgtgcggga aacggcaacc atcatgtaat tattgcggtt gagttcaact
     9181 tgcagaatac cgccaatatt gctgtccacc aaaggcagga tagtggagtt cacaaacccg
     9241 tcattggtga aagaggagaa ggagtcattg gcaatgctga cagcatcaaa gtagttggtg
     9301 agattcacca tacctgcgat gaggcaagcg cgcttccctg caaagtattg catcagcgcc
     9361 acttcaggga atattccttc atgaggcccc atcgcgtcag catgcaagcc ggaagccgtg
     9421 gcataggctt cagtgaaaaa cgtatcgctc tgagcggatt cacgatccag cccccatgaa
     9481 ccggaaaatt ccacacgcaa ccaggttccc ccgttggcct tgtcttcaat aaggcgctga
     9541 ttaagctggg catgaagaag agccaggttg ttatggcgct taacatcgga aaccttttcc
     9601 gtattccaaa gtccgtaggc catatccaca ccccattggg tgccgtattt ctcattggcc
     9661 ttcaccttgt aggcaggatc gccttcttcc ggagtgaaca tattgatgcc ctgcaaaata
     9721 tcaccaacag caggggtgct gtcttgagcg attacaggaa gcgccatgag gctcgtcaac
     9781 gccattgcta ttatgttatg cttcttcata gaaaacggtt agtcttatcg ttggatgttt
     9841 gtggtataat acttatatga ttgatccaga ggtggagagg ggcgctttcc ttttacaggg
     9901 aaagagctcc gcttccgttc tcccgggctt gctctttcac agctctgccg catgccccca
     9961 tacagaaccc acagtctccg cagagcagac ggtatctggg acgccggatg agcagcccgg
    10021 ccagtttcct gtaccggcag acccggcgga agaaacggtg aagtcctgta tgggtcaagt
    10081 tcatgcaacg cggcaaagtt agattaaact aactattcag tcaacacttt ttcttcccaa
    10141 tgcccttgcc ctgctcagta caacattatg ttggttcatt atattttatt tgttttcaac
    10201 gtattaaaaa tatcgcaaaa atattttcat gtctgctaaa aattagtttc agctaattca
    10261 tcttgaccag aatacggggg cacgctaaaa aaatgcaggc tggattggat ataatccaat
    10321 ccttccggaa aagaagcggc tgcatgccgc caaggccgga tgaacccaat caaaacatta
    10381 ccatgagtta catgaacaac aaaggcctct ggggcttcgt cggaggaatg atcgccgccg
    10441 ccatcggagc gaaagtagta aaaagtcccg ttgccaggaa attggccgtc cagggccttt
    10501 ccaaggccat gctcatccag cagaaggcca tggaacaact ggcaaacatc cgggaagaag
    10561 cccaagacct gtgccatgag gccgccgcga aaaacagcag gccggaacag tagcaatctc
    10621 attgtccatc accaaacagc ggggtgcccg tttccttctc cgccagcgga atggaccgcc
    10681 cccaacgggg gaaacgcacg gagaagggcg tttccccctt ttctttttcc ttatccatgc
    10741 attaccaggt tatccatcat acgcccggca ggctccgtgt acgcagcggc cgctgcgcat
    10801 tcaatcatga tcaggcttac ggcatcgaat gcaggctcct gaaacagaaa ggcgtatact
    10861 ccgtcaaggc caccccctgc aacggaggtg tgttcatcct gtatgaagga accagccccc
    10921 aggccatttt ccgcacgctg gacaggctca ggccggaaac actccgcagc cacgaaccgg
    10981 aagagcgcgc ggaggccagg aaactggccc ggtccttttt tctccgcata gcggggaaag
    11041 cttcctcttt cctgttttgc aaggtgtttc tgccgccccc gctcagaatg gccaaagtat
    11101 tctggaatta cagttcctat ttacggcgcg gaatggccgg cctgggaagc ggacggctca
    11161 acgtagccgt tctggacgcc gtctccatcg gggtttccat aggcacgaag gcttacggaa
    11221 cggccaattc catcatgttc cttctttcca tttcagacct gctggaaaat tacactcgga
    11281 aaaagacccg ggcggcgctt gcggccagcc tgagcataaa tatagaccgc gtctggcggg
    11341 tggaagaagg aggcatgtgc caggttccca tggagcaaat ccgcccgggg gagaaaatcc
    11401 gcgtggacgc ggggaacgtc atccctgtgg acggtaccgt gctgtccgga gaagcggaag
    11461 tcaaccaggc cgccatgacg ggggaatcgg aagcggcttc caaacgggaa gggtccgtcg
    11521 tctttgccgg aaccacgctg gaaacgggtt ccctcgtcat ccgggtggac gccgcaggag
    11581 accagtcccg cattaacaat atcatcgccc tgatcgacca ttcggaggaa ctgaaagcgc
    11641 gcatccaaag ccgggcggaa aagctggcgg attctatcgt tccctatacc ctgctcacgg
    11701 caggggcgct gtttctgttc accaggaata tctccaaggc tctctccgtg ctgatggtgg
    11761 attattcctg cgccatcaag ctcgccaccc ccatctccgt catctccgcc atgaaagagg
    11821 ccgcagcgcg caagatcatg atcaagggcg ggaaattcat ggagctgttc gccaaaacgg
    11881 acaccatcgt ttttgacaag acgggcacgc tgacctccgc ctgccctcag gtaacgcaga
    11941 tcatcccttt gagcgactgc agcagggaat atatcctgaa aacggcagca tgcctggaag
    12001 aacacttccc ccacagcgtg gcgcgggccg ttgtgcgcaa agcgctggag gagggcctgc
    12061 accatgaaga agaacacgcg gaggtggaat acatcgtggc ccacggcatt tcctcccgcc
    12121 ttcatgggaa aaaggttctt gtgggcagct accattttct ctttgaagat gaacgcatcc
    12181 ccctcacgga agagcaaagg cagaccatac gcaatcacgc cagaggcaaa tccaacatct
    12241 ttctcgctat aggccgcaga gcaatcggca tgatcggcgt cagcgacccg cccaggccgg
    12301 aagccgcaga gacgatcgcc cggctgaaac ggcagggcat ttcctccatc atcatgctca
    12361 cgggggacag cgaatccgca gcccgcgcca tcagccgcca gttgggcatc acggaatacc
    12421 gttcccaggt tctgcctgaa gataaggccc gctttatcca gcaattgaag aaatccggca
    12481 aaacagtgtg catggtggga gacggcatca acgattcccc agctctttcc tgtgcagacg
    12541 tttccgtctc catgaaggat tcttccgata tcgcccgtga agtggcggat atttccctgt
    12601 tgtccagctc tctggcagaa ctggttgttt taagggaact gagctgtgcc gtccttgaaa
    12661 aaatagaacg caactaccgc tttatcgtcg ggttcaactc ttctctgata ctgctgggca
    12721 tgttcgggct gattaccccg gccttttccg cgttcctcca caacgcttcc acagtgtacg
    12781 tcagt
//
LOCUS       DAOMSU010000037        12642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_243_length_12642_cov_3.109716, whole genome shotgun sequence.
ACCESSION   DAOMSU010000037 DAOMSU010000000
VERSION     DAOMSU010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_243_length_12642_cov_3.109716"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="PK077_02925"
     CDS             <1..275
                     /locus_tag="PK077_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRN23284.1"
                     /translation="KAVNPKVQRLVSGSTLVIPARPGDKFEPEKSAAEELEPESVPHI
                     VKKGETLSVIARKYKMNWRALQKFNKMNDRDVARLKIGQKIMIPKK"
     gene            300..1442
                     /locus_tag="PK077_02930"
     CDS             300..1442
                     /locus_tag="PK077_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRN23285.1"
                     /translation="MSSKVCMILVWFFVICLLVVGLVMVSSTAAWAEETKHPYEPLFK
                     QTAFACAGLVGAMILSRIDYRIWRKYIWWILGGACFLLVLCYVPGIGKEINGERRWIT
                     IGMQFQPSECAKLCMMMALANWLALYRDRTTSFWWGFVMPGLIFGIPLALILFEKDMG
                     TSVALALAAFCVMFVAGTRKIYLGGAFALAGTALYVLVQSNANRLERFLAWKDLDAHR
                     LGAGLQQYRASIALSRGGLDGVGLGNSAEKHGTLPFAHTDFIFAPLGEEFGFYGTMFV
                     LLCYFLMTYAGIGVAMQCRDTYGRFLAVGIVAIIFCPAILNIAVVTNAVPNSGLPLPF
                     ISFGGTNLVFTLAALGMLTSIQRFSTGAQPNCEITRKDERSIDVRL"
     gene            1439..2563
                     /gene="murG"
                     /locus_tag="PK077_02935"
     CDS             1439..2563
                     /gene="murG"
                     /locus_tag="PK077_02935"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HRN23286.1"
                     /translation="MTEPSLKHPSLNIVIACGGTGGHLFPGIAVAQELKKRGHRVTLL
                     ISQKKVDAQASKNYGDLDFRTIEAIAMPKIPSLSLLGFGVRLYKAIRFSRNLLDEVEA
                     DVVIGMGGFTSFPPVYAAHRKGIRTYVHDSNALPGKANRMTAKCCTNVLLGIEEARHY
                     FNPAKCIVTGTPVRQEMVARKDKNEARAELNLPRDRRVALVMGGSQGARNLNSLVIEA
                     ARQCADLCDFLIITGSGDFARVSQLTADMPHVHVIEFCSAMAAAYAAADVVISRSGAS
                     SLTELAHMGKAALLVPYPFAADDHQAHNARVFAAHGAARMMRENTLTPDDIAAFLNEV
                     LKDSSLLASMNECALRLDMPDAVSRIANVIEHTDHAASHD"
     gene            2556..4853
                     /gene="murC"
                     /locus_tag="PK077_02940"
     CDS             2556..4853
                     /gene="murC"
                     /locus_tag="PK077_02940"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRN23287.1"
                     /translation="MIETLRKRLTDREHPARLHLIGVAGSGMSGLALMLMEMGHRVSG
                     CDRVTSAETERLQSLGLSFSCPHSADAVSDAEIVIYSSAIRADNPALAAARKLNIPCM
                     RRAECLAAILNGKKGIVVSGTHGKTTTSALCAHLMREGRMRPCHYVGAEIPVLGTNAH
                     WNEDSEYLVAEGDESDGTLVNYIPEHSIVLNIEPEHLDHYKDLDEIKAVFDRLCSQTR
                     GKIIYCRAHPGAEDVCAKYPNSVSYGWSDADYTATDVLERRGRTLFTVLKGKEELGRV
                     ELGIPGRHNVLNSLAAIALATELGVDFPAVARGLASFAGAKRRFETKYLSPSIRIVDD
                     YGHHPTEIAATLQTARSLQPNRLVVYFQPHRYTRTQLLADDFGKVLQEADLVFVADVY
                     PASELPIEGVSGQTIINAMHRHGPVETRYLPDLGIAHHAIGNALKPGDLFLTLGAGNV
                     HECGMRIAHDLALLEDLERTAGGSLEGKLYEPMSRHTTMGVGGCAQYWLEPSTFSGMQ
                     AAVNYCRDRNIPVHVIGRGSNIIVRDGGLRGAVIHPSGGEFDVLEIQGNRLTAGAGVR
                     LKKLVSTAVQNGLGGLEWMDGIPGNVGGSLRMNAGAMGMDMVKNLVSVTCLDEDGEIR
                     SHTREELNAQYRSIPDLVHNFVLQAVFEAQPAPAEEMERLLEAARARRKLSQPVGASA
                     GCIFKNPPEIPAGKLIDELGLKGACVGDACVSDVHANFIINRGHARARDITILIDMIR
                     KEAKENRGIDLKSEAQVIGDRDPQF"
     gene            4876..5811
                     /locus_tag="PK077_02945"
     CDS             4876..5811
                     /locus_tag="PK077_02945"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HRN23288.1"
                     /translation="MNTLKRDLKITLLLGGPGAEREVSLVSGNSVYDALCRAGFTDVT
                     KVDVHGPDFRLPEGTELAYNIIHGTFGEDGTLQAILEERGVPYTGAGSRSSALCFDKS
                     ESKKVFLDAGVPTPQAEILDCSNGIVMPSLPLPFVIKPPCEGSSVGVHIVRRQEDVLP
                     AMKEAVTHGTTVLVEEFIQGKELTVGILDGKALPVIHICPRSGFYDLSNKYPWMNMGG
                     GTDYICPADLPEETAAKVQEAALKAYKAAGVEVYGRVDVLLREEDGAPFVLEINTIPG
                     MTPSSLLPKAAAASGWPYEDLCEKIAELSLATPRK"
     gene            5910..6809
                     /locus_tag="PK077_02950"
     CDS             5910..6809
                     /locus_tag="PK077_02950"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRN23289.1"
                     /translation="MLLLEREARKETIERKGKNYRFWLFRRKLYHIVTVYALIFGLIG
                     AIVFLWKDYILKYDWLSIDTVTLKSNGIFNSEQAFSVMGIGPQDNIFSIDAAELEQRL
                     KKCPAIRRASVKRQISSNPTLLVDIDARIPVAWIDCPELGIHPGDATYGALADKEGVI
                     FPCMEQVHMPYIREKRMPSVTLRPPSSGKLSYGVSIRELEAPMKLIELLSGTVTEYLP
                     SIVSITTPNDWSFCVRFTNDCQATFSHYGLEHQVEKLSRALRHARQTHRKISSINLIP
                     EHNIPVIFDNSYEDIPLAEPIEE"
     gene            7035..8075
                     /gene="recA"
                     /locus_tag="PK077_02955"
     CDS             7035..8075
                     /gene="recA"
                     /locus_tag="PK077_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRN23290.1"
                     /translation="MSNETPLSPEAEKLAAARKRNLDLALSQIQKDFGENAIMRLGDN
                     VKMEVDVIPTGNLLIDRALGVGGFARGRIVEIYGPESSGKTTLTLTAIAQAQKSGGLA
                     AFIDVEHALDPQYAARLGVNLDDLLVSQPSSGEEALQICEALVRSNAIDVIVVDSVAA
                     LVTKQELEGEIGDSTVGAQARLMSAALRKLTSFISKARTVCIFTNQIREKIGVMFGNP
                     ETTPGGRALKFYASVRVDIRRIGQIKGSDGVIAGNRTKIKVVKNKVAPPFTECEFDIM
                     YNEGISSVGSLLDLATEYDIIQKRGSWISYNGSQIAQGRDAAKEALKSNPELYAEIEE
                     QVKAKMDEKTAK"
     gene            8235..9254
                     /locus_tag="PK077_02960"
     CDS             8235..9254
                     /locus_tag="PK077_02960"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRN23291.1"
                     /translation="MYPTSRTSFAAVLGIVGMFLLTLSTAFGWNPEIINGVQYIPMSE
                     VRTHYKLTRERTEGRQKVYEVPEKIQIRIQARSQEMFMNNMKFVLSYPVADHPSKGLM
                     VSNMDLHKIIDPVLRPTYIANRRSFNTVVIDPGHGGHDSGTRNRISREADINLSVGKK
                     LRDRLKAMGYQVVMTRDTDNFIALQDRVRIANRHNNAIFISIHFNDGGSSARGVETFT
                     LAPAGTSSSMSRNIRHDALQGNAQDSMNIALATAVQGHMLKGPLAIKEGISMVDRGIK
                     RARYSVLCTIKHPAILVEGGFMSNPQEALLIATERYQNFMASSLAAAVHQYRTALGQQ
                     VRRTR"
     gene            9290..9928
                     /gene="lipB"
                     /locus_tag="PK077_02965"
     CDS             9290..9928
                     /gene="lipB"
                     /locus_tag="PK077_02965"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HRN23292.1"
                     /translation="MNIIRIPGLADYQETLRLQEQLVRAHQEDPDRENELLLLEHAGV
                     YTIGRTRNHASLHPGSVLPFPVHEINRGGQATYHGPGQLVGYPITDLNRCGRDLHRYV
                     TTLENTLIETCARFGVQARIREGLVGVWVENRKIASIGVGVKKWIAMHGFALNITRKS
                     LPPFLAITPCGIQGVQMTCLEDEMSSPLPEGNLLKCFGDVFAALWEEKFNRY"
     gene            10131..11543
                     /gene="leuC"
                     /locus_tag="PK077_02970"
     CDS             10131..11543
                     /gene="leuC"
                     /locus_tag="PK077_02970"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HRN23293.1"
                     /translation="MGKTLFQKIWDAHTVGILPDGRTQMFIATHLLHEVTSPQAFGMV
                     RDLGLTVRHPERTFATVDHIIPTDNQAEPFADATADAMIRELRRNCAENGIRFFDLPT
                     GLQGIVHMVGPELGITQPGMTIVCGDSHTATHGAFGSIAMGIGTTQVRDVLATQTLAL
                     SPLKVRRINVNGKLAPGVRAKDVALHIIGLLGAKGGLGFAYEYGGEVIDAMSMDERMT
                     LCNMSIEGAARCGYVNPDQTTVEYIKGRLFAPTGADWDKAVERWLGFASDADAEYDEI
                     VEIDGASIEPTLTWGISPDQNTGISGCTPNPSDAADDDERKMINEALEYMKFPADMPL
                     KGLPVQVCFVGSCTNGRISDFREVAALIKGRHVAPGIRALAVPGSQMTARQCEEEGIA
                     DIFREAGFEWRLAGCSMCLAMNPDKLQGDQLCASSSNRNFKGRQGSPTGRTLLMSPAM
                     VAAAALTGKVSDAREVFSLN"
     gene            11563..12171
                     /locus_tag="PK077_02975"
     CDS             11563..12171
                     /locus_tag="PK077_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HRN23294.1"
                     /translation="MSLTQFTSITGTCVPVPGPDMDTDRIIPARFLKCITFDELAGVM
                     FWDERFDENGQSKSHPVDDPRFKGASIILGGSNFGCGSSREHAPQTIRRSGIKAVIAE
                     SFAEIFFGNSTGIGLPCVCASAADIAALARRISDHPETEVTIDLLNMTASWKEGSFPI
                     RMPAEAREALTRGRWDSIAELLVNMDAVEKKNKELPQVAASC"
     gene            12216..>12642
                     /locus_tag="PK077_02980"
     CDS             12216..>12642
                     /locus_tag="PK077_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23295.1"
                     /translation="MIFRITDYVHYGTLDNRERGTVRLTLQLMGMPHPVNITLQGDCL
                     QDLAGCVVDFRNPSPQMLPAELTQIPEHIQGVAGDMTASRRMPVKGRKTMENALYLEW
                     FTSHHDMVLLESTVFSIKVSLPEWIMDSCEEQAQIMANQQ"
BASE COUNT         2760 a         3978 c         3453 g         2451 t
ORIGIN      
        1 tgaaagccgt caatcccaaa gttcagcgcc ttgtcagcgg aagcacgctc gtcattccgg
       61 cccggccagg agacaaattt gaaccggaaa agtccgccgc ggaggaattg gagccggaaa
      121 gcgtgcccca cattgtgaag aaaggggaaa ccctctccgt aatcgcccgc aaatacaaaa
      181 tgaactggcg cgccctccag aaattcaaca agatgaatga ccgggacgta gcgcgtctca
      241 aaatcgggca aaaaatcatg atccccaaaa agtaggcatc ttcctgccct tccgccgcca
      301 tgtcttccaa agtctgcatg attctggtct ggttcttcgt catctgcctt ctggtcgtag
      361 gcctggtgat ggtatccagc acggcggcgt gggcggagga aaccaagcac ccgtacgaac
      421 ccctgttcaa gcagaccgcc tttgcctgcg ccgggctggt gggggccatg attctctccc
      481 ggatagacta ccggatatgg aggaagtata tctggtggat ccttgggggg gcctgttttc
      541 tgctggtgct gtgctatgtg cccggcatcg gaaaggaaat caacggggaa cgccgctgga
      601 tcaccatcgg catgcagttc cagcccagcg aatgcgccaa gctctgcatg atgatggccc
      661 tggccaactg gctggccctg taccgggacc gcaccacctc tttctggtgg ggttttgtga
      721 tgcccggcct tatcttcggc attcctcttg ccctgattct ttttgaaaag gacatgggca
      781 cctccgtagc cctggccctg gccgcgttct gcgtcatgtt tgtggccgga acgcgcaaaa
      841 tctacctggg cggggctttc gccctggccg gaacagccct gtacgttctg gtgcagagca
      901 acgccaaccg cctggaacgt ttcctggcct ggaaagacct ggacgcccac cgcctgggcg
      961 ccggcctcca gcagtaccgt gcttccatcg ccctgtcccg aggggggctg gacggggtgg
     1021 gcctgggaaa cagcgcggaa aaacacggca cgcttccctt cgcccacacg gacttcattt
     1081 tcgcgccgct gggagaggaa ttcggttttt acgggaccat gttcgtcctg ctctgctatt
     1141 tcctgatgac gtatgccggc atcggcgtgg ccatgcagtg ccgggacacc tacggacggt
     1201 ttctggccgt ggggattgtc gccatcattt tctgtcccgc catcctgaac atcgccgtgg
     1261 taaccaacgc cgtgcccaat tccggcctgc cgctgccctt catcagcttc gggggcacca
     1321 acctggtctt tacgctggcc gccctgggca tgctcaccag catccagagg ttttccaccg
     1381 gcgcgcagcc caattgtgaa atcacccgca aagacgaacg ctccattgac gtaagattat
     1441 gactgaaccc tctctcaaac atccctccct gaacatcgtg atcgcctgcg gcggcacggg
     1501 ggggcacctt tttcccggca tagccgtagc ccaggaactt aaaaagcgcg gccaccgcgt
     1561 cacgctcctc atctcccaga agaaagtgga cgcccaggcc agcaaaaatt acggggatct
     1621 tgacttccgc accattgagg ccatcgcgat gcccaaaatc ccatccctgt ccctgctggg
     1681 gttcggcgtc aggctgtaca aagccatccg tttcagccgc aatcttctgg acgaagtgga
     1741 agcggacgtc gtcatcggca tgggaggatt cacttcattc cctcccgttt acgccgccca
     1801 ccgcaaggga atccgcacct acgtgcacga ttccaacgcg ctgcccggaa aggccaaccg
     1861 catgaccgcc aaatgctgca cgaacgtgct gctgggcatt gaggaagcca ggcactactt
     1921 caatcccgcc aaatgcattg tcaccggcac tccggtacgc caggaaatgg tggcccggaa
     1981 agacaaaaac gaagccaggg cggagctcaa cctgccccgg gaccgccgcg tggccctggt
     2041 aatgggcggt tcccaggggg ccaggaatct gaattccctg gtcattgaag ccgcccggca
     2101 atgcgcagac ctgtgcgact ttctcatcat caccggctcc ggggactttg cgcgcgtcag
     2161 ccagctgacg gcggacatgc cccatgtaca cgtcattgaa ttctgttccg ccatggctgc
     2221 cgcgtacgcc gcagcggatg tggtcatttc ccgttccggg gcgtccagcc ttacggaact
     2281 ggcccatatg gggaaggccg ccctgctggt tccctacccc ttcgccgcag acgatcatca
     2341 ggcacacaac gcacgcgttt tcgcggccca cggcgcggcg cgcatgatgc gtgaaaacac
     2401 cctcacgccg gacgatattg cggctttcct gaatgaagta ctcaaggatt cctccctgct
     2461 ggcctccatg aatgaatgtg ctttacgtct ggacatgcct gatgcagtat cccgcatcgc
     2521 caatgtcatt gaacatacgg accatgcagc ctcccatgat tgaaacctta cgcaaaagac
     2581 tgacagacag ggaacacccg gcacgcctcc atctcatcgg ggtagccggg tcaggcatgt
     2641 ccggcctggc gctcatgctg atggaaatgg ggcaccgggt gagcggctgc gaccgggtga
     2701 ccagcgcgga gaccgaacga cttcaaagcc tgggtctgtc tttctcctgc ccgcattccg
     2761 cagacgccgt ttccgatgcg gaaattgtca tttattccag cgccatccgg gcggacaatc
     2821 cggccctggc ggcagcgcgc aagctgaata ttccctgcat gcgccgcgcg gaatgcctgg
     2881 cggccatcct gaacgggaag aaagggatcg tggtctccgg tacgcatgga aaaaccacta
     2941 cttcagccct ctgcgcgcac ctgatgaggg aaggccgcat gcgtccgtgc cactacgtgg
     3001 gggcggaaat ccccgtgctg ggaaccaacg cccactggaa tgaagacagc gaatacctgg
     3061 tggcggaagg ggacgaaagc gacggcacgc tggtaaatta cattccggag cacagcatcg
     3121 tcctcaacat tgaaccggaa cacctggacc actacaagga tctggatgaa atcaaggccg
     3181 tgttcgaccg gctttgctcc cagacgcgcg gcaagattat ttattgccgc gcgcatcccg
     3241 gcgctgagga tgtctgcgcc aagtacccca attccgtttc ctacggctgg tccgatgcgg
     3301 actacaccgc tacggacgtg ctggaacgcc gcggacgcac cctgttcacc gtgttgaagg
     3361 ggaaagagga actgggccgc gtggagctgg gcatacccgg acgccacaat gtcctgaatt
     3421 ccctggccgc gattgctctg gcaacggaac tgggcgtgga tttcccggcg gtggctcggg
     3481 gcctggcctc ctttgcggga gccaaacggc gctttgaaac caaatacctt tccccctcca
     3541 tccggattgt ggacgactac ggccaccacc caacggaaat agccgccacg ctccaaactg
     3601 cccgttccct ccagccgaac cgcctggtag tttacttcca gccgcaccgt tacacgcgca
     3661 cgcagcttct ggcggatgac ttcggcaagg tgctccagga ggcggacctc gtttttgtag
     3721 cggatgtgta tcccgccagc gaactgccca ttgaaggggt aagcgggcag acgatcatca
     3781 acgccatgca caggcacggc cctgtggaaa cccgttacct tcccgacctg ggcatagccc
     3841 accatgccat cggcaacgcg ctgaaacccg gagacctgtt cctgacgctg ggagccggga
     3901 acgtccatga atgcggcatg cgcatcgcgc acgacctggc tctgctggag gatctggaac
     3961 gcactgcggg cggttctctg gaaggcaagc tgtacgaacc catgtcacgc catacgacca
     4021 tgggagtagg cggctgcgcc cagtactggc tggagccttc caccttttcc ggaatgcagg
     4081 cggccgtcaa ctattgccgg gacaggaata tccccgtaca cgtcattggc cgcggttcca
     4141 acatcattgt cagggacggc ggactgcgcg gcgccgtcat ccacccctcc gggggagaat
     4201 tcgatgtgct ggaaatccag gggaaccgcc tcaccgccgg agcgggcgtg cggttgaaaa
     4261 agctggtttc caccgccgtc cagaacggcc ttggcggcct ggaatggatg gacggcattc
     4321 ccggcaatgt gggaggaagc ctccgcatga acgccggagc catgggcatg gatatggtca
     4381 aaaacctcgt ttccgtcacc tgtctggatg aagacgggga aatccgcagc catacaaggg
     4441 aagaattgaa cgcccagtac cgttccattc cggacctggt ccacaatttt gtgcttcagg
     4501 ccgtgtttga ggcgcagccc gcccctgcgg aagaaatgga gcgtcttctg gaggcggcac
     4561 gggccaggcg gaagctttcc cagcccgtcg gagccagcgc cggctgcatc ttcaaaaacc
     4621 cgccggaaat cccggcaggc aagctcattg atgaactggg gctgaaaggc gcctgcgtgg
     4681 gagacgcctg cgtatcagac gtgcacgcca attttatcat caaccggggt catgccagag
     4741 cgagggacat caccatcctg atcgacatga tccgcaagga ggccaaggaa aaccgcggca
     4801 tcgacctgaa atcggaagcc caggtcatcg gagaccggga cccccagttt taacccattc
     4861 actatctatt tccatatgaa cactctcaag cgcgacctca aaatcaccct cctgctcggg
     4921 ggtcccggag cggaacggga agtatccctt gtttccggca actccgttta cgatgccctg
     4981 tgccgggccg gattcaccga tgtaacgaag gtggacgtgc acggcccgga tttccggctg
     5041 ccggaaggca cggagctggc ctacaacatt atccacggca cgttcggcga ggacggaacc
     5101 ctccaggcca ttctggaaga acgcggcgtt ccgtatacag gggccggctc ccgttccagc
     5161 gccctgtgct ttgacaaatc cgaatccaag aaagtatttc tggatgcggg ggttcccacg
     5221 ccgcaagcgg aaatcctgga ctgttccaac ggcatcgtga tgccgtccct ccccctgccc
     5281 ttcgtcatca agcccccctg cgagggctcc agcgtgggcg tccacatcgt ccgcaggcag
     5341 gaggatgtgc tccctgccat gaaggaagcc gtaacccacg gaaccaccgt attggtggaa
     5401 gagttcattc aaggcaagga actgacggtc ggcattctgg acgggaaagc ccttcccgtc
     5461 attcatatct gcccccgctc cggcttctac gacctgagca acaaataccc gtggatgaat
     5521 atgggcggcg gcacggatta tatctgcccg gcggatcttc cggaggaaac cgccgccaag
     5581 gtgcaggagg cggccctgaa agcctacaag gccgccggcg tggaagtgta cggccgcgtg
     5641 gacgtactgc tgcgcgagga agacggcgcc cccttcgtgc tggaaatcaa caccattccc
     5701 ggcatgaccc cttccagcct gctacccaag gcggcggcgg cctccggatg gccctacgaa
     5761 gacctttgcg aaaaaatagc ggaattatcg ctagccaccc ctcgtaaata acgtaatatc
     5821 tgacagaggg cggggagtcg cccctcccgc accccccgat gtcgcacaaa gccacaacgc
     5881 cagtcccccg ttccgccaaa aggccggaaa tgcttcttct ggaacgggag gccagaaaag
     5941 agaccatcga acgcaagggc aaaaactacc gtttttggct cttccggcgc aagctgtacc
     6001 acattgtcac cgtttacgcg cttattttcg gcttaatcgg ggctatcgtt tttctctgga
     6061 aagattacat cctcaaatac gactggcttt ccatcgacac cgtcaccctg aaaagcaacg
     6121 gcattttcaa ctccgagcag gccttttccg ttatgggcat agggccgcag gacaacattt
     6181 tttccataga cgctgcggag ctggagcagc gcctgaaaaa atgtcccgcc atccgccggg
     6241 cttccgtcaa acgccagata tcctccaacc ccacgctgct ggtggacatc gacgcgcgca
     6301 tccccgtggc ctggattgac tgcccggaac tgggaattca cccgggagat gccacatacg
     6361 gcgctttggc agacaaggaa ggcgttatct tcccctgcat ggagcaggtc cacatgcctt
     6421 atatccggga aaagcgcatg ccctccgtca cgctcaggcc gcccagttcc ggcaaactca
     6481 gctacggcgt cagcatccgg gaactggaag cgcccatgaa actgattgaa ctgctttccg
     6541 gcacggtaac ggaatacctt cccagcatcg tctccataac aacgcccaac gactggtctt
     6601 tctgtgtgcg ttttaccaat gactgccagg ctactttcag ccattacggc ttggaacacc
     6661 aggtggaaaa attgtcccgc gccctccggc acgcacgcca gacacaccgc aaaatcagtt
     6721 ccatcaacct gattccggag cacaacattc ccgtcatctt tgacaattcc tacgaggata
     6781 ttcctcttgc ggagccgatt gaagaataac gcccccagac gttctccgga aaaaccgtgt
     6841 ttttctccag ctccaaactt cttttccggc cccgttcccc ctgtttcccc taatttcccg
     6901 ccgaacggaa cacccggttt tcacaagatt cttcccatgt cagaattctg ttctttgtca
     6961 atgaggaatg agtctgcgaa gacccacaga acacttgcca ataaggctgt cccggttata
     7021 ctgcgctacg atatatgagc aacgaaacac ctttatcccc cgaagcggaa aaactggcgg
     7081 cagcccgcaa gcgcaacctt gacctggccc tctcccaaat ccagaaagac ttcggagaaa
     7141 acgccattat gcgccttgga gacaacgtca aaatggaagt ggacgtcatt cccacgggca
     7201 accttctgat tgaccgcgct ctgggtgtgg gcggctttgc ccgcggccgc attgtggaaa
     7261 tctacggacc ggaatcctcc ggtaaaacca ccctgaccct gacggccatc gcccaggccc
     7321 agaaatccgg aggcctagct gcattcattg acgtggaaca cgcgctggac cctcagtatg
     7381 ccgcccgcct gggcgtgaac ctggacgacc tgctggtttc ccagccaagt tccggtgaag
     7441 aagccctgca aatctgcgag gctcttgtcc gctccaatgc cattgacgtc atcgtggtgg
     7501 actccgtggc cgcactggtc accaagcagg agctggaagg agaaatcggg gattccacgg
     7561 taggagccca ggcccgtttg atgtccgccg ccctccgcaa gctcaccagc ttcatttcca
     7621 aggcccgtac cgtctgcatc ttcaccaacc agatccgtga aaaaatcggc gtcatgttcg
     7681 gcaatccgga aaccaccccc ggcggccgcg ccctgaaatt ctacgcctcc gtgcgtgtgg
     7741 acatccgccg tatcggccag atcaagggca gcgacggcgt catagccgga aaccgcacca
     7801 aaatcaaggt agtcaaaaac aaggtggccc ctcccttcac ggaatgcgaa ttcgacatca
     7861 tgtacaatga aggcatctcc tccgtaggca gcctgctgga cctggcaacg gaatatgaca
     7921 tcatccagaa gcgcggctcc tggatcagct ataacggcag ccagatcgcc cagggccgcg
     7981 acgccgccaa ggaagctctc aaatccaacc cggagctgta cgcggaaatt gaagaacagg
     8041 tcaaggccaa aatggacgag aagaccgcca aataatcccg cctgttcccg ttcctttact
     8101 gccatttcag ggcggctccc cttcccggga gccgcttttt tctctccgga aacagaagcg
     8161 caaaagaatc tttcacggaa acttttccct tgtgcgtcat gaaaaatccg ttagtctgga
     8221 tgcgatatcg ttctatgtat ccgacctctc gcacatcatt tgcagccgtg ctcggcattg
     8281 tgggcatgtt tctgctgacg ttgtccacgg ccttcggatg gaatccggaa atcattaatg
     8341 gagtgcagta tattccgatg tcagaagtgc gcacccatta caaactgacc agggaacgca
     8401 cggaagggcg gcagaaagtt tacgaggtgc cggaaaagat tcaaatccgt atccaggcgc
     8461 gcagccagga gatgttcatg aacaacatga agttcgtgct gtcctatcct gtggctgacc
     8521 atccctccaa ggggctgatg gtttccaata tggacctgca caaaatcatt gacccggttc
     8581 tgcgccccac ttacatcgcc aaccgccgca gtttcaacac agtagtcatt gatcccggcc
     8641 acggcggcca tgacagcggc acacgcaacc gcatcagcag ggaagcggat atcaaccttt
     8701 ccgtgggcaa aaagctgcgc gaccgcctca aggccatggg ctaccaggtg gtcatgaccc
     8761 gtgacacgga caacttcatt gcccttcagg accgcgtccg cattgccaac aggcacaata
     8821 atgccatctt catcagcatc cacttcaatg acggaggctc ttctgcccgc ggggtggaaa
     8881 ccttcacgct ggcgcccgcg ggcacttcct cctccatgtc ccgcaatatc cgccacgacg
     8941 ccctgcaggg caacgcccag gacagcatga acattgccct ggctacagcc gtccaggggc
     9001 acatgctgaa aggaccgctt gccatcaagg agggcatttc catggttgac cgcggcatca
     9061 agcgcgcgcg ctattccgtg ctgtgcacca tcaagcatcc cgccattctg gtggaaggag
     9121 ggttcatgtc caacccgcag gaggccctgc tcatcgccac ggagcgttat cagaatttca
     9181 tggcctcttc cctggctgcc gctgtgcatc agtaccgcac cgccctgggc cagcaagtgc
     9241 gcagaacgcg ctgagttccc taactgtccc ggggcctccc ttcagcagca tgaacatcat
     9301 ccgcattccc ggcctggcag attatcagga aaccttgagg ctccaggaac aactggtccg
     9361 ggcccatcag gaagacccgg acagggaaaa cgaactgttg ctgctggaac atgccggggt
     9421 ttataccatc gggcgtacac ggaaccacgc cagcctgcac cccggttccg tacttccctt
     9481 tcccgttcat gaaatcaacc gcggcggcca ggccacctac cacggccccg gccagctggt
     9541 cggatacccc attacggacc tgaaccgctg cggcagggat ctgcaccgtt acgtcaccac
     9601 cctggaaaac accctgattg aaacctgtgc ccggttcggc gtgcaggccc gcatccggga
     9661 agggctggta ggggtctggg tggaaaaccg taaaatagcc tccatcggcg tcggggtgaa
     9721 aaaatggatt gccatgcacg gcttcgccct caatataacc cggaaatccc tgcctccctt
     9781 ccttgctatt accccctgcg gcatccaggg cgtgcaaatg acctgcctgg aggatgaaat
     9841 gtcatccccc ctgccggaag gaaacctgtt gaaatgtttc ggagacgttt ttgcagcact
     9901 atgggaagaa aaatttaacc ggtattaaag gaattcggaa cgtgcggagg gcatgaaact
     9961 catcctttcc ttgaaggcgg aaaggcccca aagactggaa agaggctcat tcagaccatg
    10021 aatgcagagg aaatgttcaa aaaaacgcct tatgtaagac acagaccgtg tcagcaatac
    10081 gtccttgcat cttgacgcgg ttcccgaacg gacggatgct tatgtcagtt atggggaaaa
    10141 cgcttttcca aaaaatctgg gacgctcata ccgtcggcat cctgccggat gggagaacgc
    10201 aaatgttcat cgccacgcac ctgctgcatg aagtcacctc tccgcaggct ttcggaatgg
    10261 tccgggacct gggcctgacc gtgcgtcacc cggaacgcac ctttgccact gtggaccaca
    10321 tcattcccac agacaaccag gcggaaccgt tcgcggacgc cacggctgac gccatgatca
    10381 gggaactgcg ccggaactgc gctgaaaacg gcatccgctt tttcgacctc cctaccgggc
    10441 tccagggcat cgtgcatatg gtagggccgg aactcggcat cacccagccg ggcatgacta
    10501 tcgtatgcgg agactcccat acggccaccc acggagcctt cggctccatt gctatgggca
    10561 tcggcaccac gcaggtgcgc gatgtgctgg ctacgcagac cctggccctc agcccgctca
    10621 aggtgcgccg catcaatgta aacggaaagc tggcccccgg cgtgcgcgcc aaggatgtgg
    10681 ccctgcacat catcggcctt ctgggagcca agggcggcct gggcttcgcc tacgaatacg
    10741 gaggcgaggt cattgacgcc atgagcatgg acgaacgcat gaccctctgc aacatgtcca
    10801 ttgaaggcgc ggcgcgctgc ggctacgtga accccgacca aaccacggtg gaatacatca
    10861 aagggcgact gttcgccccc accggcgcgg actgggataa agccgtggaa cgctggctgg
    10921 gcttcgcttc cgatgcagat gcggaatacg atgaaatcgt ggaaattgac ggagcctcca
    10981 ttgaacccac gctgacatgg ggcatttctc cggaccagaa tacgggcatc agcggctgca
    11041 cgcccaaccc atccgacgca gcggacgacg atgaacggaa gatgatcaat gaagcgctgg
    11101 aatacatgaa attccccgcg gacatgcctc ttaaggggct gccggttcaa gtgtgcttcg
    11161 taggttcctg caccaatggg cgcatttcgg acttccggga agtggccgcc ctcatcaagg
    11221 gtcgccatgt ggcccccggc atcagggctc tggccgttcc cggctcccag atgactgccc
    11281 gtcagtgtga agaggaaggc atcgcggaca ttttccgtga agccggcttt gaatggcgcc
    11341 tggcgggttg ctccatgtgc ctggccatga atccggacaa gctccagggt gaccagctct
    11401 gcgccagttc ctccaaccgg aacttcaagg gccggcaggg aagccccacc gggcgcaccc
    11461 tactgatgag cccagccatg gtggccgctg ctgctctgac cggaaaagtc tccgacgccc
    11521 gcgaagtgtt ctccctgaat taactttcca attttttcct ttatgtctct gacccaattc
    11581 acctccatca ccggcacctg cgttcccgtt ccgggaccgg acatggacac ggaccgcatc
    11641 atccctgccc gcttccttaa atgcatcacg tttgacgaac tggcgggagt catgttctgg
    11701 gacgaacgct tcgacgaaaa cggacaatcc aaatcccacc cggtggacga tccccgcttc
    11761 aaaggggctt ccatcatttt gggcggctcc aacttcggct gcggctcctc ccgtgagcat
    11821 gccccgcaga cgatcaggcg ctccggcatc aaagccgtca ttgcggaatc tttcgcggaa
    11881 atctttttcg gcaacagcac cggcatcggt ttgccctgcg tgtgcgcgtc tgccgccgac
    11941 attgccgctc tggcccggcg catttccgat cacccggaaa cggaagtgac gattgacctc
    12001 ctgaacatga cagcttcctg gaaggaaggc tccttcccca tccggatgcc ggcggaagcc
    12061 cgcgaagccc tgacccgggg ccgctgggat tccattgcgg aactgcttgt caacatggac
    12121 gccgtagaaa agaaaaacaa ggagctgccc caggtcgcag cctcctgctg attcagcatt
    12181 catctctttc ctccccttcc gccctccttc cccatatgat tttcagaatt acagactacg
    12241 tccactacgg cacgctggac aaccgtgaac gcggaaccgt ccgccttacg ctgcagctca
    12301 tggggatgcc ccatccggta aacattaccc tgcaggggga ctgcctccag gatctggctg
    12361 gttgcgtggt tgacttcagg aatccctctc cccagatgct tcctgcggaa cttacccaga
    12421 tcccggaaca tatccagggg gtggcgggag acatgacggc cagccgccgc atgcctgtca
    12481 aaggcaggaa aaccatggaa aatgccctgt acctggaatg gttcaccagc caccacgaca
    12541 tggtgctgct ggaaagtacg gtcttctcca tcaaagtttc ccttcctgaa tggattatgg
    12601 attcctgcga agaacaggcc cagatcatgg cgaaccagca ga
//
LOCUS       DAOMSU010000038        12531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_252_length_12531_cov_2.559154, whole genome shotgun sequence.
ACCESSION   DAOMSU010000038 DAOMSU010000000
VERSION     DAOMSU010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12531
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_252_length_12531_cov_2.559154"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="PK077_02985"
     CDS             <1..522
                     /locus_tag="PK077_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRN23296.1"
                     /translation="PDTATAAAYLSRGNFCWNAGMFVWSIPAVCEQLDKHCPELASFV
                     EHVAESPAPQETIREEFPALTPISIDFALMEHADRVLNMEATFDWDDVGSWISVANYL
                     ETHNKNTTNTDITVQDASGNIVFSQKGDKHVALLGVENLIVVETADAILIADKNKADE
                     IKKIVNQLPDELR"
     gene            519..944
                     /gene="ruvX"
                     /locus_tag="PK077_02990"
     CDS             519..944
                     /gene="ruvX"
                     /locus_tag="PK077_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRN23297.1"
                     /translation="MTSPHPALGIDYGEARIGIAATDPVGIMAHPVETIHRHQTDGIS
                     RIVQLVQKRGIRTLVLGLPVRMDGTEGTAAAKVRAFGRELAAALPGLPLIFMDECLTT
                     VIAQEKLHAAGKKAKNFRPVIDQVAAVEILNTWLDSTLG"
     gene            complement(1135..2136)
                     /locus_tag="PK077_02995"
     CDS             complement(1135..2136)
                     /locus_tag="PK077_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-enoyl thioester reductase domain-containing
                     protein"
                     /protein_id="HRN23298.1"
                     /translation="MSENHYAEFSECSMRPQEVLEYVSGPIPVLEEGEVLVRMKAAPI
                     NPADINFVQGVYGLKPVLPHSRAGLEGCGVVQESRAAGFREGDEVILLRGVGSWSEYV
                     AVPSVNVMKLPVKVDPVQAAMLKVNPLTALRMLEGFVSLEPGDWLVQNAANSGVGRCI
                     IQLAREMGVKTVNFVRRPDELRDELTELGADLVVGEDDGDVVKNTLARLDGKRPVLAS
                     NAVGGESALRLMDILAPGGSMVTYGAMSRKSIKVPNGFLIFKGIKLEGLWVTQWLKNA
                     PVSEIEAAYDRLARLMADGRLKQAVDTVYPLSDVRKAVEKAQEEFRSGKVVLSMDCA"
     gene            2592..2855
                     /gene="rpmB"
                     /locus_tag="PK077_03000"
     CDS             2592..2855
                     /gene="rpmB"
                     /locus_tag="PK077_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRN23299.1"
                     /translation="MSRICIIRGTMPHKGRRIHRSGLAKKKGGIGRHVTKTVNRTVFP
                     NLQEKRIWVPELGQFVKMKISAKALRTINKNGAYNTLKKVGLL"
     gene            3055..3279
                     /locus_tag="PK077_03005"
     CDS             3055..3279
                     /locus_tag="PK077_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23300.1"
                     /translation="MKAQTKIALRINDDNPNHHLWNNRGVWWCHVTVHKPDSTAERLR
                     FSLKTRDIEKARARRDKIFASIQQHFGIAA"
     gene            complement(3618..5219)
                     /locus_tag="PK077_03010"
     CDS             complement(3618..5219)
                     /locus_tag="PK077_03010"
                     /inference="COORDINATES: protein motif:HMM:NF027814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HRN23301.1"
                     /translation="MRLYHFLLPAVVSAAVSASFGAEFPNPYPAPAPGVRLTPEIPLS
                     PSINGARIVGATPGSRMLFQVPVSGERPMKIQATGLPPGLKMDSRGLIAGTAPSGKRE
                     YKVNIQASNRHGKDMKELILKVGDELCLTPPMGWSSWYSYSEAVGQDNVLKTARLFVE
                     RGLVNHGWAYINIDDCWQGRRGGKYGAIQPNKRFPDMKAMCDAIHAMGMKVGIYSTPW
                     MGTYAGFIGGSAPNAKSDYGEMAIPEKERKQEDQIFGSYPGVHRRKADHVGAVWLFDR
                     DAKQWADWGFDYVKVDWNPNDVSTTKRIRKALDESGRDIVLSLSNAAPYEHVEELGKL
                     ANLWRTTGDIQDHWGSVSGIGFSQERWQKHMRPGHWNDPDILQIGKLGKPNQPNTTFV
                     QTRLTPDEQYTHVTLWCLLSAPLIVSCDLEHIDSFTMGLLTNDEVIAVDQDPAARPAR
                     KAWHQGNFQVWMKELSDGSVAAGFFNTGKEKGILKVNLKELGLSGAYEARDLWKRADQ
                     GTVQGDMAVELNGHGASMFRFSKEK"
     gene            complement(5243..6187)
                     /locus_tag="PK077_03015"
     CDS             complement(5243..6187)
                     /locus_tag="PK077_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HRN23302.1"
                     /translation="MGTVFTVRAYPGSGMDAGDAERICGEALACAAHWEKVMSAMDAE
                     SGLARLNAAEYGISVPVSPELERVLLLALDYARLTNGAFDPTLGPCIRLWKKSRRLGI
                     LPSDEEREGALRACGWEKLSVRKGMAVKAVSGMRLDLGGMGKGFAVDRMAEMLKTRGV
                     CSFFIDSTSDVLAGAPPPGEPGWRLRVDTGNGQGEVLLLSHAAVSTSGSARQMVKIGG
                     KAYSHVLDPRSGLGVTEGRQVSVRASSAALADALATAGCVMREEEFRSLAAGLPGVSV
                     PAFFQSPPCSAGETQKQDGLRKEGGMGSMKGMTKQLRF"
     gene            complement(6632..>7453)
                     /locus_tag="PK077_03020"
     CDS             complement(6632..>7453)
                     /locus_tag="PK077_03020"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRN23303.1"
                     /translation="NVMAGNTGSSSPDGSYEGLVYLPCTPENNFVPQLPDEHVDLIYL
                     CFPNNPTGAVASRNELLKWVEYARANRAIILYDSAYEAFIQDSSIPRSIFEIPGARDC
                     AIEFRSFSKQGGFTGVRCGYVVIPRELHGYDSEGNKVSISRLWSRRTSTKFNGASYIV
                     QRGAAALFTMEGMAQTAALISHYLGNASLLLNACRQAGMRVWGGENAPYVWVQCPDGL
                     DSWQMFDKMLHEANVVITPGSGFGSRGEGFFRISAFNSRENVDEVCRRIHSLFAR"
     assembly_gap    7454..7534
                     /estimated_length=81
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7535..7942)
                     /locus_tag="PK077_03025"
     CDS             complement(<7535..7942)
                     /locus_tag="PK077_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HRN23304.1"
                     /translation="MPNINDNFLKLQAGYLFPEIGRRVHAFAESHPEAAKRLIRCGIG
                     DVTEPLPMAAIEAMHLAVDDLSTHERFHGYGPEQGYFWLREAIAKKAYQVHGVHVEVD
                     EIYVSDGAKCDTGNILDIFGTGNKIAVPDPVYPV"
     gene            8373..9653
                     /gene="eno"
                     /locus_tag="PK077_03030"
     CDS             8373..9653
                     /gene="eno"
                     /locus_tag="PK077_03030"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRN23305.1"
                     /translation="MEIIDVRGREIIDSRGNPTVEVDVALAGGAIGRASVPSGASTGE
                     HEAWELRDGDAKRYGGKGVLKAVENINKIIAPEISGHDATLQPAIDKIMIDLDGTPNK
                     SRLGANAILGVSLAVAKAAAIQLNLPLFKYLGGPNAKVLPVPMMNIINGGAHSDSPID
                     FQEFMIMPKGAPTFRESLRYGAEVFHALKDVLHDRGLSTAVGDEGGFAPALKSADDAL
                     ECIAQAVKRAGYTLGTDIFIALDVASSEFYDPSRNLYVFKKSDGQGRTAEELTAYYQE
                     LQKKYPIISIEDGCAENDWLGWEQLTKAMGGNTQLVGDDLFVTNVEFLNQGISRHVAN
                     AVLVKVNQIGSLTETLDTVELAKDNKYSAIISHRSGETEDATIADIAVATNAGQIKTG
                     SLSRSDRMAKYNQLLRIEEELGNDAVYGGKIHIL"
     gene            9682..10050
                     /locus_tag="PK077_03035"
     CDS             9682..10050
                     /locus_tag="PK077_03035"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRN23306.1"
                     /translation="MLWRRRYYHRFTLAELEIRREKRALIVQRALRLFAIVLALCLTM
                     LLSLVCFKPWKDLRSLEHERSFLQAHLEKSREQMEQAKNEFIWISQDPHYFEMIARDK
                     GNLALPGEHILRIAEPKRLK"
     gene            complement(10166..11473)
                     /locus_tag="PK077_03040"
                     /pseudo
     CDS             complement(10166..11473)
                     /locus_tag="PK077_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(11818..12429)
                     /locus_tag="PK077_03045"
     CDS             complement(11818..12429)
                     /locus_tag="PK077_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23307.1"
                     /translation="MEDVNMAADMVLCRSCNKISKFSEIVQEERDEEILDSIPRRMSV
                     TKTARGLEITYKKPKGAGLFLLMFSVFWNSVTCVALFAVRKEMSCENLFGLLFLIPFV
                     LVGAGTFAGAFYVLFGRMTLILTPGRGELFRGVGNLGRRQHFLLEKDSRISIEASSMR
                     RNDEVLDKVVVGLPDGKTFEFGTGIEEVEAQQYIAALLRKMRG"
BASE COUNT         3012 a         3884 c         2973 g         2581 t
ORIGIN      
        1 ccggacacgg ccacggctgc agcctacctg agccggggca atttctgctg gaacgccggc
       61 atgttcgtat ggagcattcc tgccgtgtgc gaacagctgg acaagcactg cccggaactg
      121 gcctccttcg tggaacacgt tgccgaatct ccggccccgc aggaaaccat tcgggaagaa
      181 tttccggcgc tcacccccat ttccatagac ttcgcgctca tggagcatgc ggacagagtg
      241 ctgaacatgg aagcaacctt tgactgggac gacgtaggtt cctggatatc cgtagccaac
      301 tatctggaaa cccacaataa aaataccacc aacacggaca tcaccgtgca ggacgcctcc
      361 ggcaacattg ttttctccca aaagggggac aagcacgtgg ccctgctggg agtggaaaac
      421 ctgattgtgg tggaaacggc ggacgccatc ctcattgcgg ataaaaacaa ggcggacgaa
      481 atcaagaaaa tcgtcaacca gctgccggac gaactcagat gaccagtccg caccccgcct
      541 tgggcatcga ttacggagaa gcccgcatcg gcattgccgc cacggacccc gtgggcatca
      601 tggcccatcc cgtagaaact atccacaggc atcagacgga cggaatttcc cgcattgtcc
      661 agctcgtcca gaaacgggga atacgcaccc tggtgctggg tcttcccgtc cgcatggacg
      721 gcacggaagg aactgccgcg gccaaggtgc gcgctttcgg aagggaactg gccgcagccc
      781 ttcccggcct tcccctgatc ttcatggatg aatgcctcac caccgtcatt gcgcaggaaa
      841 aactgcacgc cgccgggaaa aaggcaaaaa acttccgccc cgtcatcgac caggtagccg
      901 ccgtggaaat actcaacacc tggcttgatt ccacgctggg ctgaagccaa cggcgcgcac
      961 gctccgtccc tcccggctct acgctcctac ggaactcctt acttcccgtt tcctgagcaa
     1021 gaccattctc ccggcaaaca aaaacgccgc cagccaacgc ccccaaccac ggaacgctga
     1081 aaacgtcctt tcagcattcc gcagtacggg aaaacaaagc tccctttccg cgcatcaggc
     1141 gcaatccatg ctaagcacca ccttgccgct gcggaactcc tcctgagcct tctccacagc
     1201 cttccgcaca tcgcttagcg gataaacggt atccacagcc tgcttcaacc tgccgtccgc
     1261 catcaggcgc gccagcctgt cataggcggc ctcaatctct gaaacagggg cattcttaag
     1321 ccactgcgtc acccacagcc cctccagttt aatacccttg aaaatcagaa aaccgttcgg
     1381 caccttgatg ctcttccggc tcatggctcc gtacgtcacc atgctcccgc cgggagccag
     1441 tatatccatc aggcgcaggg cgctttctcc gcccacggcg ttggaagcca gcacaggcct
     1501 ctttccatcc aggcgggcca gcgtattctt caccacatcc ccgtcatcct cgcccaccac
     1561 cagatcggcg cccagctcag tcaattcatc cctcaattca tccggccttc tcacaaaatt
     1621 cactgtcttc acgcccattt cacgggccag ttgaataatg caccttccca ctccggaatt
     1681 ggcggcattc tgcaccagcc aatcccccgg ctccagggaa acgaaccctt ccagcatgcg
     1741 cagagcggtc aggggattca ccttcagcat ggccgcctgg acgggatcta ccttcaccgg
     1801 gagcttcatg acattcacgg agggaaccgc cacatactcg ctccaggaac ccacgccgcg
     1861 caggagaatc acttcatctc cctctcgaaa tcccgctgcg cgagattcct gtaccacgcc
     1921 gcagccttcc aggccggcgc gggagtgcgg cagcacgggc ttcaggccat aaactccctg
     1981 tacaaaattg atgtccgccg ggttgatcgg agccgccttc atccggacca gaacttctcc
     2041 ctcctccaga acgggaatgg ggccggaaac gtattccagc acctcctggg gcctcatgct
     2101 gcattcggaa aactctgcat aatgattctc actcatgctt tcactttctc atggaacgcc
     2161 ccttcgtgca agcgccggat acgttttacc cgggagcagt cagccatatg ccgctgccgt
     2221 gaaaaaaaca aagggggcaa tctttcccgt gttctgcact tttcctgcga tgaaaggcct
     2281 tcatccgacc cggctatccg tccagcccct gctcccacag cggaagtcct gaaaaaatat
     2341 ggaagaacgc ctcattctct tctttgtctg cagtaccgtt catcttcccg gatcagcccc
     2401 ctcccgcttt cagctcaccg tctctgcagc ccctcttcat catgggagaa aaaaccggaa
     2461 ttcgtgcaaa agaaaaggca tgcttccgta cagtccagga taaattccag tgctttcgtc
     2521 cggttgttct tgaccttagg caaaaatacg ctagattatc tctctcgcat taacaaataa
     2581 cttcgattgc catgtcccga atttgcatca tcagaggtac catgcctcac aaaggtcgtc
     2641 gtatccatcg ctccggtctt gccaagaaaa agggcggtat tggtcgtcac gtcactaaga
     2701 ctgtcaatcg taccgttttc cccaaccttc aggagaagcg catctgggtt cctgaactcg
     2761 gtcaattcgt gaaaatgaag atttccgcca aggccctgcg tacgatcaac aagaacggtg
     2821 cttacaacac tcttaaaaaa gtaggtctcc tgtaaaaaag attctttttt tgaacgttgt
     2881 tccaccactt gaactctaag gcttcaacga ggtgagcaac tggcagaaaa agatgtccaa
     2941 aaagacaagg gatcatctgc ttaagtgagg cacctccaag ttgaaggttt tattgaaaca
     3001 ttaatatgcc aacggacgta aacaatacgt cctctactgg aaaagatcac acagatgaaa
     3061 gcccaaacca aaattgctct tagaattaat gatgacaacc ccaaccacca cctgtggaac
     3121 aacagggggg tttggtggtg ccacgttacg gttcacaagc cggactccac ggcggaacgc
     3181 ctgagattct cgctgaaaac ccgtgacatt gaaaaagccc gcgcccgtcg cgacaagatt
     3241 ttcgcctcca ttcagcagca tttcggcatt gccgcctaga aaagctgtcg ggagagatta
     3301 aggcaagtca catcaatctc ttttataaga accgctccgg atctgccgga gcggtttttt
     3361 attacacaat aacaaaggcc tgccggagaa aactttccga aattgaagcg caagctctgg
     3421 tgcgggggag gcattcactg cccctcctgc ccctcctgga atccgacaat ccgccgacag
     3481 gtactgtaaa agtgggattt tgtgcagaag gcttgtttct atggatgccg gttcaatgaa
     3541 aagccgcccc tgccacaggc ttcaggggcg gcatatcccc gccgcccctg aatgaataca
     3601 gagccgtctc cttcccgtta cttctctttg ctgaaccgga acatggatgc tccatgcccg
     3661 ttcaattcta ccgccatatc tccctgtacg gtcccctggt cagcgcgttt ccagaggtcc
     3721 cttgcctcat acgctccgga aagccccagc tccttcagat tcaccttcaa aattcctttc
     3781 tccttcccgg tattgaaaaa gccggccgcc acggaaccgt cggacaactc cttcatccac
     3841 acctggaaat tcccctggtg ccacgctttg cgggcgggac gggcagccgg atcctgatcc
     3901 accgctatca cctcatcatt ggtaagcagt cccatcgtaa acgaatcaat atgctccaaa
     3961 tcacaggaga cgatgagcgg agcggacagt aggcaccaca gggtcacatg ggtgtactgt
     4021 tcatccggag tcagccgcgt ctggacaaac gtggtgttgg gctggttggg tttgcccagc
     4081 ttcccgatct ggagaatgtc cgggtcattc caatgtcccg ggcgcatatg cttctgccag
     4141 cgttcctggg aaaaaccgat gccgctgacg ctgccccagt gatcctggat atcccccgtc
     4201 gtccgccata aattcgccag cttgcccagc tcttccacat gttcgtacgg ggcggcattg
     4261 gacaggctga gcacgatatc cctcccggac tcgtccagcg ccttgcggat gcgctttgtc
     4321 gtagacacat cgttgggatt ccaatccact ttcacataat cgaaccccca atccgcccat
     4381 tgtttagcgt cacggtcaaa cagccagacg gctcccacat gatccgcctt tctgcgatga
     4441 acccccggat aacttccaaa gatttgatcc tcctgcttgc gctccttttc cggaatggcc
     4501 atttccccgt agtccgactt agcgttgggc gcgctccctc cgataaaacc ggcatacgtt
     4561 cccatccaag gcgtggaata aatgcccact ttcatgccca tggcgtgaat agcgtcgcac
     4621 atggccttca tgtcaggaaa acgcttattg ggttgaatgg cgccatactt ccctccgcgc
     4681 ctgccctgcc agcagtcgtc aatgttgata taggcccagc catgattgac cagaccccgt
     4741 tccacaaaaa gccgtgccgt cttcagcaca ttatcctgcc ctacggcctc actgtaggaa
     4801 taccagctgc tccagcccat gggcggagtc aggcacaatt cgtcccccac cttcagaatc
     4861 agctccttca tgtcctttcc atgcctgttg gaggcctgga tatttacttt gtattccctc
     4921 ttcccggacg gagcggtacc cgcaatcaat ccgcgcgaat ccatcttcag gcctgggggc
     4981 agccctgttg cctgaatttt catgggccgc tccccggaga cgggaacctg gaacagcatg
     5041 cgggagccgg gggtagcccc gacgatacgg gcgccattaa tggagggtga aagcggaatc
     5101 tctggagtca ggcggacacc gggagcgggc gcaggatagg gattgggaaa ctctgcccca
     5161 aatgatgcgg atacggcagc actgacaacg gcagggagta aaaaatggta caatctcata
     5221 aacagggtta taaaggtgta ccttaaaaac gtagttgctt tgtcattcct ttcattgacc
     5281 ccattcctcc ctccttccgc agtccatcct gtttctgcgt ttcccccgca gaacatggcg
     5341 gactttgaaa aaaggccgga acggacactc ccggcaatcc ggccgccagg gaacggaact
     5401 cctcctcacg catcacacac cctgccgtcg ccagggcatc cgccagcgca gcggaagacg
     5461 cccggacgct cacctgcctc ccttccgtaa cgcccagccc cgaccgggga tcaagcacgt
     5521 gggaatacgc tttcccgcca attttcacca tctgacgggc gctcccggaa gtggaaacgg
     5581 ccgcatggct caacagcaac acttccccct gcccatttcc cgtatccacc cgcagccgcc
     5641 atcccggttc cccgggagga ggagcgccgg ccagaacatc gctggtactg tcaatgaaga
     5701 aggaacatac accccttgtt ttcaacatct ccgccatccg gtccacagca aagccctttc
     5761 ccatgcctcc caaatccagc ctcattccag aaaccgcctt gaccgccatt cccttacgga
     5821 cggacaactt ttcccatccg catgcccgga gcgcgccttc ccgttcctca tcggacggaa
     5881 gaatgccaag gcggcggctc tttttccaca gacggatgca gggccccagc gtgggatcaa
     5941 aagcgccgtt cgtcagccgg gcataatcca gggccagaag aagcaccctt tccaattccg
     6001 gagaaacagg cacggaaatg ccgtattccg cagcattaag ccgggccaga ccgctttccg
     6061 catccatggc ggacatcact ttttcccaat gggcggcaca ggccagagcc tccccgcaaa
     6121 tcctctccgc atctcccgcg tccatgccac tcccgggata agcgcgcacc gtgaacaccg
     6181 tacccataac cgccccccga actgttaccc tcccatccgg ggagtgcgcg caggaaagcg
     6241 gctccacaga agggagctcc gcttcccctt cctgcccccg gatggcgcca gcaacagaca
     6301 gcaggcaggc gcaacatgca ggaaaagcag cctgccggaa aaaggaactc atgcgcaccc
     6361 acggggacat aagcaacaac ctactccgta caaatgaacg ggggcaaggg ccgaatatgc
     6421 cataggaacg cccctttacc ggaatcccat cacttgaaaa cggactctgc tctttcccgg
     6481 tccaaatccg taagaaagga agatgacctt tttacaggag ccccctcccg gcaggaaatg
     6541 caatgccacg gtcatcaact cacccgaaaa gaaatacaca ggccgcggtg ttttgcaccg
     6601 cggcctggac aatctgtctg aaaacgccct tctatctggc aaacaggctg tggatgcggc
     6661 ggcagacttc atccacgttt tcccgtgaat taaacgcgga aatgcggaag aatccttccc
     6721 ccctggaacc aaaaccggaa ccaggcgtaa taaccacatt cgcctcatgc agcatcttgt
     6781 caaacatttg ccagctgtcc agaccatccg gacactgtac ccatacataa ggggcgtttt
     6841 ccccgcccca cacgcgcatg cccgcctggc ggcaggcatt cagcagaagc gaagcattcc
     6901 ccaggtaatg gctgatgagc gcggcggtct gggccatgcc ctccatggtg aacagagccg
     6961 cggcgccccg ctggacaata taggaagcgc cgttgaattt cgtgctggtg cggcggctcc
     7021 agagtcggct gatagaaacc ttgttccctt cggaatcata tccgtgcaat tccctgggaa
     7081 tcaccacata accgcagcgc acccctgtaa agcctccttg cttggagaag gaacggaact
     7141 caatggcgca atcccgcgcg ccgggaattt caaaaatgga tctgggaatg gaagaatcct
     7201 gtatgaaagc ctcataagcg gaatcataaa ggataatggc acggttcgcc cgggcgtatt
     7261 ccacccattt cagcagttca ttccgggaag ccacggctcc ggtgggattg ttcgggaaac
     7321 acaggtaaat caaatccaca tgctcatcag gcaattgcgg cacaaaatta ttttccggag
     7381 tgcacggcag atacaccagc ccttcataag aaccatccgg gctggaactc ccggtattcc
     7441 cggccatgac attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncacggg ataaaccggg tccggcacgg
     7561 cgattttgtt gccggtcccg aaaatatcca gaatgtttcc cgtgtcgcat ttagctccgt
     7621 cagaaacata aatctcatcc acttccacat gcacgccgtg aacctgatag gcttttttgg
     7681 cgattgcctc cctcagccag aaatacccct gctccggacc gtagccgtga aaacgttcat
     7741 gagtggacaa atcatccacg gccaggtgca tggcttcaat agccgccatg ggaagcggct
     7801 ccgtcacatc gccgatgccg cagcgaatca gccttttggc cgcctccgga tgggattcgg
     7861 caaacgcatg tacgcggcgc cctatttccg ggaacaaata tcccgcctgc aacttgagga
     7921 aattatcgtt aatgttaggc atggttttaa tagtaagcgc ctcattccat acccggaacg
     7981 ggacggaatc aaggcccctt tccggcatgc cgtccttccc tgcgtcacga aacggtgcgt
     8041 cttcccctga tgctgtttcc accgtgccaa agcggctgct cctgtccctc tgcgtttcat
     8101 gctcctcacc aaacgtcttg tcttccctga tttccctgag tttctttccg ggcaagattc
     8161 ccaaaaatgg gcaggcagat gcccggcaag aaaaaacctt cactctgcgg ggcattatgg
     8221 tcctgaagac gccggcaatc ttcatccccc cttctgcgcc agtcctgctt ttgaaaccgg
     8281 aacttccagg gacatgacat gcttctccgg aacggaaaaa cccgtttccc tgtctaaaaa
     8341 gaacaagaaa aaatcttatg atcatgaatg aaatggaaat catcgacgtc cgcggccgtg
     8401 aaattatcga ctcacggggc aatcccaccg tggaagtgga cgtggccctc gccgggggag
     8461 ctatcggacg cgcatccgtc cccagcggag cctccaccgg agaacatgaa gcctgggaac
     8521 tccgggatgg agacgccaag cgctacggag gcaagggcgt gcttaaagcc gtggaaaaca
     8581 tcaataaaat cattgccccg gaaatatcag ggcacgatgc caccctgcag ccggccattg
     8641 acaaaatcat gatcgacctg gacggcacgc ccaataaatc ccgcctgggg gccaacgcca
     8701 tcctgggcgt ctccctcgcc gtggcgaaag ccgccgccat tcagctcaac cttccccttt
     8761 tcaaatacct gggcgggccg aacgccaagg ttttgcccgt ccccatgatg aacataatca
     8821 atggtggagc ccattcggac tcccccatcg actttcagga attcatgatc atgcccaagg
     8881 gggctcccac tttccgggaa tccctgcgct acggggcgga agttttccat gcgctcaagg
     8941 atgtgctgca tgaccgcggc ctttccactg ccgtggggga tgaaggggga ttcgccccgg
     9001 ccctgaaatc agcggacgac gccctggaat gcattgcaca ggccgtaaaa cgggccgggt
     9061 acaccctggg cacggatatc ttcattgccc tggacgtggc ttcctccgaa ttctatgatc
     9121 cctcccggaa cctgtacgtc ttcaaaaaat cagacggcca aggcagaacg gcggaggaac
     9181 tgacggctta ctatcaggaa ctccagaaaa aataccccat catttccatt gaggacggct
     9241 gtgcggaaaa cgactggctg ggctgggaac agctcactaa agccatgggc ggcaataccc
     9301 agctggtagg ggacgacctg ttcgtgacga atgtggaatt cctgaatcag ggcatttccc
     9361 gccatgtagc gaatgccgtc ctggtgaaag tcaaccagat aggttccctg acggaaacgc
     9421 tggatacggt ggaactggcc aaggataaca aatactccgc catcatctcc caccgctccg
     9481 gggaaacgga agacgccacg atcgcggata ttgccgtagc gacgaacgcg gggcaaatca
     9541 aaaccggctc tctaagccgt tccgaccgca tggccaaata caatcaattg cttagaattg
     9601 aagaagaact gggaaatgac gcagtttatg gtggtaaaat tcatattcta tgatcaaaaa
     9661 tatgttgttt gactgcaagg aatgctctgg agacgccgct actaccaccg ttttaccctg
     9721 gcggagcttg aaatccgccg ggagaaacgt gccctgatcg tccagagagc cctgcgcctc
     9781 tttgccattg tcctggccct gtgcctgacg atgctgcttt ccctggtgtg tttcaaacca
     9841 tggaaggatc tgcgctccct ggagcatgaa cgttccttcc ttcaggctca tctggaaaaa
     9901 tccagggagc agatggaaca ggcaaaaaac gaattcatct ggatctcaca ggatccccac
     9961 tactttgaaa tgattgcccg ggacaaaggc aacctggccc ttcccggaga gcacattctc
    10021 cgcattgcgg aacccaagcg cctgaaataa agcgtttttc agcggaaaaa ggaaaccatg
    10081 cacgcggttc ctccccctct cccgccgtag gaggttccca aacgctggca tgcaggacgg
    10141 aatacggaac aaagccaacg cagccttact caggctgcct gtccggcgca gtcttgtcat
    10201 actccctcat caatttggcg gaccagctaa tactgtcggg aaaacggcat gcaggcgtcg
    10261 tagtcaggta tgttcccagc tttttagcga ggggatccgc atcctggtgc ctggaaaaat
    10321 acaaatccct ctgttcctgg ggatcatccg ccacattgta aaactgcgga gcgcgatccg
    10381 gtaccagcag gtacttcata tcaccggcca gcacagcgga cgtataatcc gtacaccaga
    10441 aaaaggtgcg cggaacggaa gctcccttgt tccccaccag ctccagaata tccatgccgt
    10501 ccagcttcct gggaatgtga cggccattcg ccttcagcaa agcgggaagc aaatccacgg
    10561 aggaaacagg ctgtctgcaa acgcttccgg gaatcaaacg cttgtccgcc ggatatctga
    10621 taataaaagg tacgcgaaca cctccctcaa aatgctgcct cttggcaccc cggaaagggg
    10681 cattgcagga agaagcttcc ggagcgcctc cattgtccga caggaagacg acgatggtat
    10741 ccttttccaa accgtccgct ttcaaggcat ccagaatgcg gcctattccc ctgtccatcg
    10801 catataccat ggcgcaataa acccgtcgtt ccccgttctg cacgttcctg aatttggcga
    10861 tatcctccgg tttggcttca ttgggtcaat ggggggcatt gtaggaaacg aacatgaaaa
    10921 aaggtttctt atcctttccg gcttcccgca gaaattcgac cgcccggtct gtaatatcgt
    10981 ccgtcaaata ggtgatgtcc gtcttatccg taaaattgga tacaatcatg gagggattca
    11041 ggccttcagc ctctttcttc acgggaaaat aatgccggga accgcccagg aacccccacc
    11101 aatgcgtaaa accgcgctcc ggaggcgtat atcccttcgt gtggccaaga tgccacttgc
    11161 cgaataccgc gctccggtac ccgcaggggg ccaaatactc cggaatcaat ttttccgtct
    11221 gcggcagccc gtagtgggac tccggaagat aatccatctg gatattggga ttcgtcgtga
    11281 tgccgtaacg cttggggaaa cgccccgtca gcaatcccat gcgggaggga gaacacatcg
    11341 gtgccgtcac atacgcccga gagcaaaaca cgccctcctt ggccagccgg tcaatggaag
    11401 gcgttttaat ctgcctggaa ccagtacagc ccaaatcccc ataacccagg tcatccgcca
    11461 gaatgacaat catgttcggc ggtgtgggaa gacggggctg cgcaactgcc gttccgcaaa
    11521 acaaaccgca cagaattaac aaaaggcgca cttccttcat catacgttaa tctacgtacc
    11581 ttattctcca tatcaatcaa aaaacctcat tcccggaatt cccgctatca tgcctcaaat
    11641 ggaaaccctg cccgctgtcc atggattttg ccaacaaaaa aggcttgcgc ccattccttc
    11701 ccggaaacgg aaatccatat ccccgctccc ttccctccag gacggattcc cctgcctgct
    11761 gaaaaacagc ggagccaggg cattccaaga ccttctccgc attcaggcat cacagcccta
    11821 tcccctcatt ttccgcagca gagccgctat atactgctgg gcctccactt cctcaatccc
    11881 tgtaccaaat tcaaacgtct tgccgtccgg cagccccact acaaccttgt ctaaaacttc
    11941 atcattccgt cgcatactgc ttgcctcaat ggaaatacgg gaatctttct ccagcaaaaa
    12001 atgctgtcgg cggcccaggt tccctactcc tcggaacaat tcccccctgc ctggcgtcag
    12061 gatcagagtc atacggccaa acaacacata aaaagctccc gcaaatgtac cagccccaac
    12121 cagaacaaaa gggattaaaa acaatagtcc gaacagattt tcgcaagaca tttccttcct
    12181 caccgcaaac agggcaacgc atgttacaga attccaaaaa accgaaaaca tcagtagaaa
    12241 aagtcccgct ccttttggct tcttatacgt tatttccagc cccctcgccg ttttcgtcac
    12301 gctcatgcgg cggggaatgg aatcaagaat ttcctcatcc ctttcctcct gaacaatctc
    12361 tgaaaatttg cttatcttat tgcaggagcg gcataacacc atatctgcag ccatattcac
    12421 atcttccata gccagaaagg cgccgcatgc caggcacttc cattcagtgt ggatagccat
    12481 atcgggcact ctaacaaact tccatattcg ttaaagaata aaaaacatag c
//
LOCUS       DAOMSU010000039        12356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_257_length_12356_cov_2.790261, whole genome shotgun sequence.
ACCESSION   DAOMSU010000039 DAOMSU010000000
VERSION     DAOMSU010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12356
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_257_length_12356_cov_2.790261"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PK077_03050"
     CDS             complement(<1..316)
                     /locus_tag="PK077_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23308.1"
                     /translation="MRERNRKKRLLLREARPLEYENGREIDADEHSDFLSGLMFREDF
                     EREMESLEPQERELVEALIDAEGNLSRAARSIHLEESRARRLRKRLREEKFTFLRNGR
                     RIS"
     gene            complement(1004..1909)
                     /locus_tag="PK077_03055"
     CDS             complement(1004..1909)
                     /locus_tag="PK077_03055"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRN23309.1"
                     /translation="MSATPNSLFRHYLDSLIQQGTTRTSLTDEARNVLRTWYVASRQG
                     GRPAASAPASPEPPPLQAAAPVSAPAGKPEPPELFPQNREPASAITLPEGTMEDKLQY
                     LRNLAQNWKPARELNSLRDTMVFATGNPHTKLMLIGEAPGYYEEVQQEPFVGRAGEKL
                     NQILRAMGLSRDMVYISNIVKFRPALPNQRTNNRAPTPEEIEACLPIIMHEIRVIRPK
                     MIIALGGTAAVGLLGEQGSVSSMRGRFHDLNGIPVRVTYHPSYLLRSDSPREKRKVWE
                     DMLAVMERLDMPISEKQRGYFLPRE"
     gene            2110..3246
                     /gene="thiH"
                     /locus_tag="PK077_03060"
     CDS             2110..3246
                     /gene="thiH"
                     /locus_tag="PK077_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HRN23310.1"
                     /translation="MSFSEELNGLMDCPTPLVRRFMALLEPVDDARLEAMAQESRRLT
                     RLHFGRTIRLFAPIYLSNECINNCKYCGFSRDNPIIRTTLTVDEVVQEARYLHGLGLR
                     SILLVAGEHPKFVSDGYMQECLDALHSFIPSLGLEIGPLPDDRYAEIVRHGAEQLAVY
                     QETYNREVYETLHTAGMKKNFNWRLDCPERAYQGGFRRIQIGALFGLSPWRREAMALA
                     VHLDYLQKHCWKSALSVAFPRMRPYAGNYEYEPDPDLMLDDRHFVQLMAALRICFPKI
                     GMSISTREPAPMRNALMHLGMTHMSAIARTEPGGYTGVGTAAAHLTVRGNRVDLPDGR
                     KGNCKATEQFEISDQRTPEQVVGAIRNAGLEPVWKDWDAALDVV"
     gene            3413..5236
                     /locus_tag="PK077_03065"
     CDS             3413..5236
                     /locus_tag="PK077_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRN23311.1"
                     /translation="MENLFTVLFEYRNMDFGLLPLLKTAYVFVTEFAVTMCYWLIPYA
                     VYLWILPRGKAGGKADRWLTSAWFFLFVLANLFEDVAEAFFWNEFEASFNFIAVDYLV
                     YTKEVIGNIYESYPIIPILGGILAVSVLAVWGMKRFLLPRNGAVPAGWKRGCVVLFLL
                     ACVTGGYWLVDIKDADAVNNRYNSEMAKDGLYSLFSAFLRNELDYRAYYRTLPDAEAA
                     AFLAREFTADDTSVPEASSGSVKRQVRPSGVAIRPNVVVVVMESMGAEFLNECREDGA
                     DVTPCLSRLGKEGIFFPNTYATGTRSVRGLEAISTSLPPLPGMSILRQEGNEHLQTIG
                     SIFRDKGYDLKWIYGGYGYFDNMNYFFGNNGFQVLDRNSMDDSEVTHSTIWGVCDEDL
                     FRRAVREADESCGRGKPFLQVVFTTSNHRPYTYPEGRIDIPSHTGRMGAVKYADYAVG
                     AFVEEARTKPWFDNTLFVFVGDHGAGSAGKQALNPETHRIFSIFYAPALLKPERRETP
                     VSQIDVLPTLLGLLKWPYDAAFYGKDALKPSYQSRYFVSNYQYIGYLKGKDMVVLKPQ
                     RRVEFFRDGKAVEPDGRMKELEKEAVYYYQHASDWRTNLKE"
     gene            complement(5299..6456)
                     /locus_tag="PK077_03070"
     CDS             complement(5299..6456)
                     /locus_tag="PK077_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23312.1"
                     /translation="MFKQLLSAFFSLFFLGAASGTSFAEVTVPDALKDRIALKKTARQ
                     LNIVYFLGSDTEPVPDYERRLSELLLYLQQFYGKEMQRHGYGARSFGLDIKSPGRVNI
                     IEYKAKNPAAHYPYENGGGWKAAQELDEFFKAHPDRKKSQHTLIIMPTWNDEKNGPDN
                     PGGVPFYGMGRNCFALDYPAFDIKHLGQKTREGRLLTKWYGGMAHELGHGLNLPHNHQ
                     TASDGKKYGTALMGSGNYTFGTSPTFLTPASCALLDACEVFSVTPAQQFYEGRPEVEV
                     KDVAISFKGDQILVSGNYTSPQTVKALNVYIQDPPYAVNQDYDAVSFSRRLGKKSGKF
                     SMKIDKKELEGLNNKEFRISLMFILANGLHMQKHFTFHWDALQDYRDGNKS"
     gene            6672..9032
                     /locus_tag="PK077_03075"
     CDS             6672..9032
                     /locus_tag="PK077_03075"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HRN23313.1"
                     /translation="MAVSAVSFGWGALDKPSASNLIWSDEPAVVVYPQEDKNSQGSFG
                     KYRKPASVWEAEGYPIGNGRVGAMIFSAPGRERLALNEISLWSGGANPGGGYGYGPDA
                     GTNQFGNYLPFGDLFVDFKKGDQPASLSVEDFTRSLDLRDGIHKVNYKADGVTYDREA
                     FSSTPANVLVLNYKASKPGQFSADFSVNSQLGADISAKGPVITWKGILKNGMNYEGRV
                     LIRPKGGTLSAAGDKISVKNADSCMVVIAMETDYLMDYKKDWKGESPARKLDRYAAKA
                     ASADYAALKQAHISQYKSMFDRVKVNFGKTEEDVAKLPTPKRLEAYKKNPADPNLEET
                     MFQFGRYLLLSSSRPGTLPANLQGLWNDYVKPPWACDYHNNINVQMAYWGAEPANLSE
                     CHEALVNYVEAMAPGCRDASQANKGFNTKDGKPVRGWTVRTSQNIFGGNGWQWNIPGA
                     AWYALHIWEHYAFTGDRKYLEKQAYPLMKEICHFWEDHLKELGAGGEGFKTNGKDPSE
                     EEKKDLADVKAGTLVAPNGWSPEHGPREDGVMHDQQLIAELFSNTIKAARILGKDAAW
                     AKSLEGKLKRLAGNKIGKEGNLQEWMIDRIPKTDHRHTSHLFAVFPGNQISKLKTPKL
                     AEAARLSLEWRGTTGDSRRSWTWPWRTALWARLGEGNKAHEMVQGLLKFNTLPNMLTT
                     HPPMQMDGNFGIVGGICEMLVQSHAGGLDIMPSPVEAWPEGSVKGLKARGNVTVDFSW
                     KDGKVSSVKLYSAQPKVLPVRVNGKMTRMKTLPLKSEAESSQPAAR"
     gene            complement(9278..10651)
                     /locus_tag="PK077_03080"
     CDS             complement(9278..10651)
                     /locus_tag="PK077_03080"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HRN23314.1"
                     /translation="MDLRSMASLYEEALSAAREEGPSAVREHSVSNHSALPDERTLQL
                     LLLEGVFGTSFTDDSGRDVHILDFGNWNKSAGPDFLNARICINGVPQSGDIELDPTPE
                     DWERHGHGSNPGFNGVILHLACAPSRRKWFTRNARHERVPLAIIPPAALARSGTSPSG
                     SAPIRHCRHSGLLASMAPEFLETLLQSAAAYRFRNKHRRHAERAKYAGEEQALFENLA
                     ETLGYHANKTAMRHLALRAPLRSIRNCPEALLFGTAGFLLPVLPASCTPEAVELHKKL
                     WAQWWPLRAQFELAPDRSFPWTYSGNRPANHPQRRVGALAVIAADFDAFKRLCLAGHT
                     EELTKYLSSLTHPYWSTHVTLPSPPSPRPMALMGRDRILDFIINHLLPMKDDEHAWKH
                     YLSLRAGQAGTKVLSVHQSLLGGRPDAQAFLAKAWHHQALLQIHDDLCSLHSCAICSL
                     LGQMEKK"
     gene            complement(10669..11196)
                     /locus_tag="PK077_03085"
     CDS             complement(10669..11196)
                     /locus_tag="PK077_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HRN23315.1"
                     /translation="MNNNSSILFGLFDEFLWVRCSGRGSFANSPTVKSLGDDYIASGG
                     RLIVIDLETCSGIDSTFMGTLAGLSRRLLPIGGSVQIASPSERCLSALESLGLDALLS
                     IEPPTAPWRGKVEQIRAALGTENAPSVHLDAKEQTLHVLNSHLTLSEMSEENEEKFRN
                     VTDCLKEELERQKDQ"
     gene            complement(11345..>12356)
                     /locus_tag="PK077_03090"
     CDS             complement(11345..>12356)
                     /locus_tag="PK077_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRN23316.1"
                     /translation="CEEAVVKSLRWLKKQQNGNGSWGEGQFRASMTGISLLAYLAHCE
                     TPLSEEFGENVLKGISFLVDLGMKNPVLSEQPALKEICYDHAVATYALCEAYTFCKQM
                     DIPSISNLEKVVVKAVDRIIDAQCPNGGWGYSYNNKINPDIDLSVTGWNVQALKAAEH
                     GGIKPTKGEIRSTLRKASSYVRKNVMPDGKFSYKENGNMPRSSLVGVGVLSLQMTGDG
                     SGGAARKGLDWIKNNVKTLQWGQGSGTENVKSNLYMHYYCVQAAMNRGGDVWTSYNKA
                     FRDAVLSGQNADGSFRKNDVGYVSGMSNKSENIYRQCLATLMLETYYRFLPGTGEGTK
                     NS"
BASE COUNT         2597 a         3157 c         3731 g         2871 t
ORIGIN      
        1 cggaaattcg ccgcccattt cttaaaaaag tgaatttttc ttcccggaga cgtttccgga
       61 ggcggcgcgc cctgctttcc tccagatgaa tgcttctggc tgcccgtgac aaattccctt
      121 cagcgtcaat caatgcctca accagctccc tttcctgcgg ctccagggat tccatttccc
      181 gctcgaagtc ttcccggaac atcagcccgg aaaggaagtc cgaatgttcg tcagcgtcaa
      241 tttcccggcc attttcatat tcaagcggac gggcttcccg cagcagcagc ctcttcttcc
      301 ggttcctctc gcgcaaatag cgttccggct caaaaacggt tgcattgccc aggtatgttt
      361 ttatgccggc tcccccactt ttccggtaac ggcggcaaat ttcccagcat cggattctga
      421 accgttggag gacatccaga tattcttcct caggtatctg aaacccgatg gattttgctt
      481 ttgatcggag gcgctgagga accgttgaag gcgaaacggg atcttcgcct cacgacacgg
      541 cgcggtatca ggtatttcac cggagttttg atgcggaggt gtccggaatt gaaaaaattt
      601 ccggtccggg aactgacgcc ggaacatggc actcattttc aggaggttat gtttgcatcc
      661 cacaggtagc gttacaaggg ccgggcggtg atgaggattt gatggagagg ggaatttcgt
      721 aaggtcccgg accagtaaaa agggaggaat tctgaatgtc tccgttcatg ctgtgcttcg
      781 gcggctcctg gcgggatacg gggcaggggg acggcctgtt caatcctccc aattagccga
      841 tgcgttagag gttgctccgg gaaaaagcgg gatggggtgg tacaaccgct ttggagaata
      901 gaatgcagat gcacggagac atactgatgt ttcctgtcac gccaaatggt tcagggattt
      961 ttccctgctt cagctgaaaa tgaggcatga agaagtcccc ggcttattcc ctgggcagga
     1021 agtagccgcg ctgtttttct gagatgggca tgtccaggcg ttccataacg gccagcatgt
     1081 cttcccatac cttgcgtttt tcccgcggac tgtctgatct gagcaggtag ctgggatggt
     1141 aagtgacgcg tacgggaatg ccgttaaggt cgtggaacct gcctctcatg gagctgacgg
     1201 aaccctgttc ccccaacagg cctacggcgg cggtaccgcc cagggctata atcattttcg
     1261 gccggatgac ccggatttca tgcatgatga tgggcaggca cgcttcaatt tcttccggcg
     1321 tgggggcgcg gttattggtt cgctggttgg gcagggccgg gcggaatttg acgatgttgg
     1381 aaatgtaaac catgtctctg gagagcccca tagcccttaa tatctggttg agtttttccc
     1441 cggcacggcc cacaaagggc tcctgctgca cttcttcata atatccggga gcttccccga
     1501 tgagcatgag tttcgtatgg ggattgcccg tggcaaagac catggtgtcc cgcagggagt
     1561 tgagttcccg cgccggtttc cagttttgcg ccagattacg gaggtattgc agcttgtctt
     1621 ccattgtccc ttccgggagg gtaatggcag aggccggttc cctgttttgc ggaaacagct
     1681 ccggcggttc cggctttccg gcaggtgcgg agacaggagc ggccgcctgc aggggcggcg
     1741 gttccggaga agcgggtgct gatgcggcgg gacggcctcc ctgtctggaa gccacgtacc
     1801 atgttctgag aacgtttctg gcttcatccg tcaggctcgt tcgcgttgtt ccctgctgga
     1861 tcagggaatc aagataatgg cggaaaaggc tgttcggtgt agcgctcatg ggaggataaa
     1921 gccagattag tccataagag gccgtctgtc acgacgattc gcaggctccc cggccttcat
     1981 ggaacgcgcc tctccgggaa atgacacggt aaaagatggt atcttttgca agggagggag
     2041 caaattggag ctttccggtt caggaatgga aatgcagccg gcgctctcac aattttactt
     2101 agttcacgta tgtctttttc tgaagaatta aacggtttga tggattgtcc cactcccctg
     2161 gtgcgccgtt tcatggcgtt gctggaaccg gttgacgacg cccgcctgga ggcgatggcg
     2221 caggagagcc ggcgtctgac gcggctgcat ttcggccgga ccattcgcct gttcgccccc
     2281 atttacctgt ccaacgaatg catcaataat tgcaagtact gcggtttttc ccgggataat
     2341 cccattatcc gcaccacgct tacggtggat gaagtggtgc aggaggcccg ctacctgcac
     2401 ggcctggggc tgcgcagcat cctgctggtg gccggggagc atcccaagtt cgtttccgac
     2461 gggtatatgc aggaatgcct ggacgccctg cattctttta ttccgtccct ggggctggag
     2521 atagggccgt tgccggacga ccgctatgcg gagatcgtcc gtcacggggc ggaacaattg
     2581 gccgtgtatc aggaaaccta taaccgggaa gtgtatgaaa ccctgcatac ggcagggatg
     2641 aagaaaaatt tcaactggag gctggactgc ccggaacgcg cctaccaggg cggtttccgc
     2701 cgcattcaga taggagccct gttcgggctt tcgccgtggc ggcgggaggc catggctctt
     2761 gccgtccatc tggattacct gcagaagcat tgctggaaat ccgcgctttc cgtggcgttt
     2821 ccgcgcatgc gtccctacgc cgggaattac gagtatgaac ctgatccgga cttgatgctg
     2881 gatgaccgcc attttgtcca gctcatggcc gccctgcgca tctgcttccc caaaataggc
     2941 atgtccatca gcacccgtga gcccgcgccg atgagaaatg ccctgatgca tttgggcatg
     3001 acccacatgt ccgccattgc gcgcacggaa ccggggggct acacgggcgt gggaacggct
     3061 gccgcccatt tgacggtgcg gggcaaccgg gtggatcttc ccgatggccg gaaagggaat
     3121 tgcaaggcga cggagcagtt tgagatttcc gaccagcgca caccggagca ggtggtcgga
     3181 gccatacgga atgccgggct ggaacctgtc tggaaagact gggatgccgc tctggatgta
     3241 gtgtaggaga atcttctccc gtctcatttc tccggaatga cattcatatc accgtgatgt
     3301 tgtgatggag cattgacggc atgctccggc tgtcatattt ccggtagcga tgattcgtta
     3361 ttattggggg aggttttggc cgttcctcct gtttgcgttc ggcgttgagg cggtggaaaa
     3421 tctatttacg gtattgttcg aataccgcaa catggatttc gggttgcttc ccctcctgaa
     3481 aacggcgtac gtttttgtga cggagtttgc cgtcaccatg tgctactggc ttatacccta
     3541 tgccgtttat ttgtggattc tgccgcgcgg gaaggcggga gggaaggcgg acaggtggct
     3601 cacctccgcg tggtttttcc tgtttgtgct cgccaatctg tttgaagatg tggcggaagc
     3661 ctttttctgg aacgagtttg aggcaagctt taatttcatt gcggtagact acctggttta
     3721 caccaaggag gtcatcggga atatttacga gtcctatccc atcattccta ttctgggcgg
     3781 cattctggcg gtgtccgttc tggccgtctg gggaatgaag aggttcttgc ttcccaggaa
     3841 cggggcggtt cccgccggat ggaaacgagg ctgtgtggtg ctgttcctgc tggcctgcgt
     3901 cacgggagga tattggctgg tggatatcaa ggatgcggat gccgtgaaca accgttataa
     3961 ttcggaaatg gccaaggatg gcctttacag cctgttcagc gcctttctca ggaatgaact
     4021 ggattaccgc gcctattacc ggacgctgcc ggatgcggaa gcggcggcgt ttctggcccg
     4081 ggagttcacg gcggatgaca cgtccgtgcc agaggcttcg tccggcagcg taaagaggca
     4141 ggtgcgtcct tccggggtgg ctatccgccc gaatgtcgtg gttgtggtca tggagagcat
     4201 gggagcggaa tttttgaacg agtgccgtga agacggggct gacgtcactc cgtgtctgag
     4261 ccgtctggga aaggaaggca tttttttccc gaacacttat gccacgggca cccgttccgt
     4321 acgcgggctg gaagcaatca gcacatccct gccgccgctt cccggcatgt ccatccttcg
     4381 ccaggaagga aatgagcatt tgcagaccat aggttccata ttcagggaca agggatatga
     4441 tctcaaatgg atttacggcg gctacggata ttttgacaac atgaattatt ttttcgggaa
     4501 caacgggttt caggttctgg accgtaattc catggatgat tccgaggtga cccattccac
     4561 tatttggggc gtttgcgatg aagatttgtt ccgccgcgcc gtacgggagg cggatgaatc
     4621 ctgcggacgc ggcaagccgt ttttgcaggt ggtgttcacc acgtccaacc accgccccta
     4681 cacgtatccg gaagggcgca ttgacattcc ttcccacacg gggcgcatgg gggccgtgaa
     4741 atacgcggat tatgcggtag gcgcctttgt ggaggaggcc agaaccaaac cctggtttga
     4801 caacacgctg ttcgtgttcg taggagacca cggcgccggg agcgcgggaa agcaggccct
     4861 caatccggaa acgcaccgca ttttttccat tttctacgct ccggctctgc tgaaaccgga
     4921 acggagggaa actcccgtga gccagattga cgtgctgccc accctgctgg ggctgttgaa
     4981 atggccgtat gatgcggcct tttatggaaa agatgctctg aagccttcct atcagtcccg
     5041 gtattttgtg agcaattacc aatatatcgg ttatttgaag gggaaagaca tggtggtgct
     5101 caaaccccag cgcagagtgg aatttttccg ggacggaaag gccgttgagc cggacgggcg
     5161 gatgaaagag ctggaaaagg aagcggttta ttactatcag cacgcttctg actggcgcac
     5221 caatttgaag gaataaggta cccgcaaggt gcggaggccg tgattgtggc ggcctccgcc
     5281 cgcgcacgga aaatgcgttc aggatttgtt tccgtccctg taatcctgaa gagcgtccca
     5341 gtggaacgtg aaatgcttct gcatgtgcag cccgttggcg agaatgaaca tgagggaaat
     5401 gcggaattcc ttgttgttca atccttccag ctccttttta tcaattttca tggagaattt
     5461 tccgctcttt ttcccaaggc gccgggagaa ggaaacggcg tcatagtcct ggttgaccgc
     5521 ataaggagga tcctggatgt aaacgttcag agctttaacg gtctgggggc ttgtataatt
     5581 gccggaaacc agaatctggt ctcctttgaa ggagatggct acgtccttga cttccacttc
     5641 cggcctgcct tcgtagaatt gctgggccgg ggtgacggag aatacttcac aggcgtccag
     5701 cagggcgcag ctggccggag tcaggaacgt gggactggtc ccgaacgtgt aattgcccga
     5761 acccatcagg gctgtgccgt attttttacc gtccgaggcg gtctggtggt tgtgcggcag
     5821 gttaaggccg tgccccaatt cgtgagccat gcctccatac catttggtca gcagccttcc
     5881 ttccctggtt ttctgcccca ggtgtttgat gtcgaaggcc ggataatcca gggcgaagca
     5941 gttgcgcccc atgccgtaaa agggaactcc gccgggattg tcggggccgt tcttttcgtc
     6001 attccaggta ggcatgatga taagggtatg ctggcttttt ttcctgtccg gatgggcttt
     6061 gaaaaattcg tcaagttcct gggccgcttt ccagccgcct ccgttttcat aaggataatg
     6121 ggccgccggg tttttggcct tgtattcaat gatgttcacg cggcctgggg atttgatgtc
     6181 caggccgaag gaacgcgcgc catagccgtg ccgctgcatt tccttgccgt aaaactgttg
     6241 gaggtaaagg agcagttcgc tgaggcgccg ttcataatcc ggaacgggtt ccgtatcact
     6301 gcccagaaaa taaacgatat taagctgccg ggccgttttt ttcagggcaa tccggtcttt
     6361 cagggcgtct ggaacggtga cttccgcaaa ggatgtaccg gaggccgctc ccagaaagaa
     6421 cagagaaaaa aatgcggaga gaagttgttt aaacatactt gccaaggtag ggacgtcgtt
     6481 attaaagaca aggaaaatta ctgttctcgc cgccattgga ggaagcgttg ccgggccggg
     6541 ctgggaataa cgggggccgt cttgtggcgg tcaataattt gggaatggct gaaagataga
     6601 ggttgcgcag atagtagata tgtgacattg ttcactaaag catgcatttc caatctctat
     6661 atttatctgc cgtggcggta agtgccgttt ctttcgggtg gggggctctg gataagcctt
     6721 ccgcctccaa tttgatctgg tctgatgaac cggccgtagt tgtctatccg caggaagaca
     6781 agaattccca gggcagtttt ggcaagtaca ggaagccagc ctctgtttgg gaagcggagg
     6841 ggtatcccat tggcaacggt cgcgttggag ccatgatttt cagcgctcct ggccgcgagc
     6901 ggctggccct gaatgaaatc agcctctggt ccggaggcgc caatccgggg ggagggtacg
     6961 gttacgggcc tgatgccggg acgaaccagt tcggcaacta tcttcctttc ggggatttgt
     7021 ttgtggactt taaaaaaggc gaccagccgg cttccctgtc tgtggaagat tttacgcgct
     7081 ccctggatct ccgggacggc attcataaag tgaattacaa ggcggacggc gtaacgtatg
     7141 accgggaagc attctccagc acgcctgcca acgtattggt gctgaactat aaagccagca
     7201 agcccggcca gttcagcgcg gatttttccg ttaacagcca gcttggagca gatatttccg
     7261 ccaaaggccc cgtcatcacc tggaagggga ttttgaaaaa cggcatgaat tatgaaggcc
     7321 gcgttttgat ccgtcccaaa ggcggtacgc tttccgccgc aggagataaa atttccgtga
     7381 aaaatgcgga ttcctgcatg gtcgtcatcg ccatggagac ggattacctg atggattata
     7441 aaaaggactg gaagggggaa tctcccgcca ggaagctgga ccgttatgcg gccaaagctg
     7501 cttctgcgga ttatgccgcc ctgaaacagg cccacatttc ccagtacaag tccatgtttg
     7561 accgggtgaa ggtcaacttc ggaaaaacgg aggaggatgt agccaagctg cctacgccga
     7621 agcgtctgga agcctataag aaaaatccgg cagatcccaa tctggaggaa accatgttcc
     7681 agttcggccg ctatctgctg ctgtccagtt cccggcccgg tacgctaccg gccaacctgc
     7741 aagggttgtg gaatgattat gtcaaaccgc cgtgggcctg cgactaccat aacaacatca
     7801 acgtccagat ggcgtattgg ggggcggaac ccgccaatct ttccgaatgc catgaggccc
     7861 tggtcaatta tgtggaggca atggcccccg gctgccggga tgcttcccag gcgaacaagg
     7921 ggttcaatac caaggacggc aaacccgtgc gcggctggac ggtgcgcacc tcccagaata
     7981 tcttcggcgg caacggctgg cagtggaaca ttcccggtgc ggcctggtat gcgctgcata
     8041 tatgggaaca ttatgcattt accggtgata gaaagtatct ggaaaaacag gcgtatcccc
     8101 tgatgaagga aatctgtcat ttctgggaag accatttgaa ggaactggga gccgggggcg
     8161 aagggttcaa gacaaacggc aaggatccga gcgaagagga gaagaaggat ctggccgatg
     8221 tgaaagccgg aactctggtg gctcccaatg gatggtctcc tgaacatggg ccgcgtgagg
     8281 atggtgtgat gcatgaccag cagctcattg cggagctttt ctccaatacc atcaaagccg
     8341 cccgcattct gggcaaggac gccgcctggg ccaagagcct ggagggcaag ctgaaaaggc
     8401 tggccggcaa taagataggc aaggaaggga atcttcagga atggatgatt gaccgcattc
     8461 ccaagacgga ccaccgccat acgtcccacc tttttgctgt tttccccggc aaccagatca
     8521 gcaagctcaa gacgcccaag ctggcggaag ccgcccgcct ttccctagaa tggcgcggca
     8581 cgaccggaga cagccgccgt tcctggacat ggccgtggcg cacggctctg tgggcccgcc
     8641 tgggcgaggg gaacaaggct catgaaatgg ttcaaggtct cttgaaattc aacactttgc
     8701 cgaatatgct gactactcat ccccctatgc agatggacgg caacttcggc attgtaggcg
     8761 gcatttgtga aatgttggtg caatcccatg ccgggggcct ggacatcatg ccctctcccg
     8821 tggaagcgtg gccggaaggc tctgtgaagg ggctgaaagc ccggggcaac gtcacggtgg
     8881 atttttcctg gaaggacggc aaggtaagca gcgtgaagtt gtattccgcc caacccaagg
     8941 tgttgcccgt gcgcgtcaac gggaagatga cccgcatgaa aaccctgccg ctgaaatcgg
     9001 aagcggaatc ttcgcagccc gcagccaggt aaaagaggaa atccatgatc cggttttaat
     9061 tccggaacgc aacataatca tggcttccgg tcctttcgta agggctggaa gcctttttta
     9121 tcccggacct ttgcgggagg aacggtgttt gtttcaaagg atgagcatgc tgaacggcgc
     9181 gttctgaaag aaggaatgcc tctccggata gaaaggttgg gcagggggga gggaaaggtc
     9241 ttccgcttcc cgccttgagg aaagaagggg gtcgctctca tttcttttcc atttgcccga
     9301 gaagggagca aatggcgcag gaatggagag agcacaggtc atcgtggatt tgaagcaaag
     9361 cctggtggtg ccaggctttc gcaagaaagg cttgtgcatc cggcctgccg cccagcaggg
     9421 attggtggac ggagaggact tttgttcccg cctgtccggc ccggagggaa agataatgct
     9481 tccatgcatg ttcgtcatct ttcatcggga gaaggtggtt gatgatgaag tccagaattc
     9541 tatcccggcc catcagggcc atggggcggg gagacggtgg ggaaggaagc gttacatggg
     9601 tgctccagta ggggtgtgta agggaggaga ggtacttcgt cagctcttcc gtatgtccgg
     9661 caaggcaaag acgcttaaac gcatcaaaat ctgctgcgat gacggccaga gcccctacgc
     9721 gcctctgcgg atggttggcc ggcctgttgc cggaatacgt ccagggaaag gagcggtccg
     9781 gcgccagttc aaattgcgcc cgcaggggcc accattgagc ccagagtttt ttgtggagtt
     9841 ctacggcttc cggtgtgcag gaggccggaa gaacgggaag gaggaagccc gccgtaccga
     9901 aaagcagggc ttccggacag ttccggatgg aacgcagggg cgcccgcagg gccaggtggc
     9961 gcatggcggt tttgttggcg tgatagccca gcgtttccgc caggttttca aaaagggcct
    10021 gttcctctcc tgcgtatttg gcgcgttccg catggcggcg gtgcttgttc cggaacctgt
    10081 aggctgccgc tgattggagg agtgtttcca gaaactccgg agccatggat gccagcagtc
    10141 cggagtgtcg gcagtggcgt atcggggcgc tgccggaagg ggatgttcct gaccgcgcca
    10201 gcgcagcggg gggaatgata gccagaggaa cgcgttcatg cctggcattg cgggtgaacc
    10261 atttcctgcg gctgggcgcg caggcgaggt gcaggatgac gccgttaaac cccgggttgg
    10321 aaccgtgccc gtggcgttcc cagtcttccg gcgtgggatc cagttcgata tctcccgatt
    10381 gcgggacgcc attaatgcat atcctggcgt ttagaaagtc cggtccggcg gatttattcc
    10441 agttcccgaa atccagaatg tgaacgtccc tgcctgaatc atccgtaaaa gaggttccaa
    10501 atactccttc cagaagaaga agctgaagag tccgctcgtc cggaagggcg gaatgattgg
    10561 aaacggaatg ttccctgacg gcggacggcc cttcttccct tgctgcggaa agggcttcct
    10621 catacaggga ggccatggag cgcaggtcca tgtggagggg aggtattttt attgatcttt
    10681 ctgccgttcc aattcttctt tcaggcagtc ggttacgttg cggaattttt cttcgttctc
    10741 ttcgctcatt tcgctcaggg tcaaatggga gttgagaacg tggagagttt gctccttggc
    10801 atccaagtga acggaagggg cattttcagt acccagggcg gcacgtatct gctctacttt
    10861 tcccctccag ggggctgtgg gcggttcaat gctgagcagg gcgtccagcc ccaggctttc
    10921 cagggcgctc aggcagcgtt cgctcgggga agcgatttgg acggatcccc cgatgggaag
    10981 cagccgtcgg gacaggcccg caagagtgcc catgaaggtg gagtcaatgc cggagcaagt
    11041 ttccagatca atgacgatca gacgccctcc ggaagcgatg taatcgtccc ccagggattt
    11101 gacagtcgga ctattggcaa aacttccgcg tccggaacac cggacccaaa gaaattcatc
    11161 aaaaaggccg aaaaggatgg aagaattgtt attcacgtcg ttagaaaatg gaaaccatat
    11221 gaataacaag agaaacctct gcggtcaagt caggatttaa tttgcgcgcc gccggaagaa
    11281 ggttcgtttt ctcgcatgca gaaccttttc ccgcggcgct cttttcgtta ttcggaggga
    11341 tgcctcagga gttcttggtg ccttcccccg taccgggcag gaaacggtaa taggtttcca
    11401 gcatcagcgt tgccaggcac tggcggtaga tgttttcaga tttattgctc atgccggaaa
    11461 cgtatcctac gtcatttttc cggaaactgc cgtccgcatt ctggccgctg agcacggcgt
    11521 cccggaatgc tttgttgtaa gaggtccata cgtctcctcc gcggttcatg gcagcctgca
    11581 cgcagtagta atgcatgtag agattactct tgacattttc cgtgccgctt ccctggcccc
    11641 actggagggt cttgacgttg ttcttaatcc agtccaatcc cttgcgggct gcaccgccgg
    11701 agccgtcgcc cgtcatttgc agagaaagca cgcctacgcc taccagggag gaacggggca
    11761 tattaccgtt ttccttatag gaaaatttgc cgtccggcat gacgttcttg cgcacgtagc
    11821 ttgaggcctt gcgcagcgta gagcggattt cccctttggt aggcttgatg ccgccgtgtt
    11881 cggcagcctt gagagcctgc acgttccacc cggtgacgga aaggtcgatg tccggattaa
    11941 tcttgttgtt gtagctgtag ccccagcccc cgttgggaca ctgggcgtca ataatcctgt
    12001 ccacggcttt tacgacgacc ttttccagat tggagatgga ggggatgtcc atctgtttgc
    12061 agaaggtata ggcttcgcac agggcatagg tggctaccgc gtggtcataa cagatttcct
    12121 tcagggcggg ctgttccgaa aggacggggt ttttcatgcc taagtccacc aggaaggaaa
    12181 tgcctttcag gacgttttct ccgaattcct cggaaagagg ggtttcacag tgggccaggt
    12241 aggccagcag ggaaatgccg gtcatggaag cgcggaattg cccttcaccc caggagccgt
    12301 tgccgttctg ctgttttttc agccagcgga gggatttgac tacggcttcc tcgcac
//
LOCUS       DAOMSU010000040        12227 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_262_length_12227_cov_2.401167, whole genome shotgun sequence.
ACCESSION   DAOMSU010000040 DAOMSU010000000
VERSION     DAOMSU010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12227
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_262_length_12227_cov_2.401167"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="PK077_03095"
     CDS             complement(<1..1216)
                     /locus_tag="PK077_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23317.1"
                     /translation="MKYAPVRDLSQLPMDGGWTYGEVLDKAAAGDFRANMLAGEYWMG
                     LYYLNRGDLPLACLEHMQHFFRDAATASGHPAPKLMMTDPYGIFGIFKAQPMQPEHAE
                     WLAACRKLAEKGDMDAVTALYTVTDLPEAELRRYLAPLEKKAASGDQDALAQLAFIKV
                     DWLKENPANVIETLKQYRKNAKPVLLARIGELALALDGKDGRGWRDTAIQSLSRAAEK
                     GHAGAMRLLAELTKGAAPAQAGKLLAELRSRGDLETLLADLSAGMKEKTGGGKSSLLQ
                     PLFEQARKLGSNNACAWYAQLISEAQPPQEQELRKAALDLLSLHDERGMPYLKEPLSF
                     PQKKLNQLPPYAKTHKELELASQLGDTDSSVQLGELWKEELANTLDSVGKAHEARENM
                     RVWYRQAAEEGHP"
     gene            complement(1598..2728)
                     /locus_tag="PK077_03100"
     CDS             complement(1598..2728)
                     /locus_tag="PK077_03100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRN23318.1"
                     /translation="MPATGTPLPWDRGNGPCVMAPPHLRGWAEEAAAFLSLRGMDGIT
                     AFATSGSSGSPPKAILFTRRALDICARGALEHLHAESGDWCCPLPLWHVGGAMIYLRA
                     ALAGTAVHTLRGKWCPQAYADLMKSSGARWSSLVPTQVVDLVSLGLRAPSTAGCIIVG
                     GGALDTETGRKARTLGWPVVQSYGMTEAGSQLATALPGDSFHTDRLSLLPHWEAQTDK
                     GGLLRFQGKGKLFGRLLTQSHGGFLLERVAPQEWWSTRDLVRLEGRLLTFLRRADRLV
                     KVLGELVDPDAVQDVLRRSVPEAVVEAVPHPRAGMELVACGPSAAPLKQACREWNASA
                     PGPQRIRAVMETPIPLTVMGKLDRNRLRSQLSDHPEKLKGIY"
     gene            complement(2741..3052)
                     /locus_tag="PK077_03105"
     CDS             complement(2741..3052)
                     /locus_tag="PK077_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23319.1"
                     /translation="MNHHMDKATEERLRAEAWVGDAILALYVREWILAEEGAINGKLF
                     VEFTSNDFLRRTGNATGVEAEIGRTFKAGGLEAAYAWIEHHLKPRMEERWHTLRRKAL
                     R"
     gene            complement(3049..3849)
                     /locus_tag="PK077_03110"
     CDS             complement(3049..3849)
                     /locus_tag="PK077_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23320.1"
                     /translation="MTRLILPICIISLLSGCQDANPAEREWKEQLYKNLAIVGARNWI
                     VIAESSFPAYTGTGIRTMVSDKTSDEVLLDVLNMLEEEAHVVPRIMISSELRSITEDY
                     APGIKRYRNNINKMLPGRQHFELMSRTINSLIEDAARQFNVLVIKTKTSLPYSNIYIE
                     LDSGYWNSESETVLRKSLEARDAANRRAAQDRVLDVPLVPGAAPAPQGSKENPPIPGA
                     PASPADNLPELPRENRQPASPSGDKAPGVPPPPIRDPLGRKTAIARFS"
     gene            complement(3885..4469)
                     /locus_tag="PK077_03115"
     CDS             complement(3885..4469)
                     /locus_tag="PK077_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRN23321.1"
                     /translation="MLSLESQIKTAADNAQNRFGKDIITDRYEFRGDITLTVARQAVA
                     DVVRWLRDSAGFDMLVNLSSVDNMGESPRFEVNYTLTQSETGANLSLKTKVDDGEEVP
                     SVTEIFQSANWHEREAWDLMGIKFSGHPDLRRILMWEGYPYFPLRKDFPVQGVPTELP
                     GVAFTEAAPTQGAPFATEQGRCPSACREPRSHKF"
     gene            complement(4515..5096)
                     /gene="nuoB"
                     /locus_tag="PK077_03120"
     CDS             complement(4515..5096)
                     /gene="nuoB"
                     /locus_tag="PK077_03120"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HRN23322.1"
                     /translation="MVTKNEPNIYETGVLDSNAEGNFVYTTLDAAINWIRKNSLWPMP
                     MGLSCCAIEFMAVACSRYDLSRFGSEVTRFSPRQADVMIVAGTVTYKMALAVRRIWDQ
                     MPEPKWCIAMGACASTGGMFRSYSVLQGVDKILPVDVYISGCPPRPEAILEALLTLRK
                     KLDTQQPARTFFKKEEPREANAPVPVNTEMPLE"
     assembly_gap    5600..5649
                     /estimated_length=50
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(5755..6768)
                     /locus_tag="PK077_03125"
     CDS             complement(5755..6768)
                     /locus_tag="PK077_03125"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRN23323.1"
                     /translation="MIRLSDHIAAAGGWLSLEAFMQLALHHPQEGYYSSSIENIGQRG
                     DFSTTPTLSPILAKAIVAHWKEACSRCGRRLPLLEIGAGSGALAVKILERLGFWNRLN
                     TDYVIVESSPRLREFQHLLLGGRAKIYSTMEKALKHCGGKAFIFSNELVDAFPARVFE
                     YTEQDWKEVGLVVKNGAVREELRPVRQQPLFSHMLEYGSQPGQRVEIHDSYARWFTSW
                     LPLWNMGVMTVIDYGDEMERLYYRRPRGSLRGYKSHQVLTGEELYRNPGLTDLTCDVN
                     FTDLLELSRNCLEDRVTFMTQRDYLLPHAENTPQDAFLTDEYGAGGHFHVLIQERQRL
                     QPE"
     gene            complement(6785..7720)
                     /gene="nadA"
                     /locus_tag="PK077_03130"
     CDS             complement(6785..7720)
                     /gene="nadA"
                     /locus_tag="PK077_03130"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HRN23324.1"
                     /translation="MAPFDLKEEILRLKEERNAIILAHSYQTGDIQDMADYVGDSLGL
                     AYKAQNTDCDVIAFCGVHFMAETAKILNPDKTVILPDADAGCSLEASCDAGELEAFLK
                     ENAHKNYYVVAYVNCSIGVKALADVIVTSGNAVKIVSQAPQDRPILFVPDQNLGAWVS
                     RQLGRPLELWNGACHVHMEFTRDQILALKEKHPGVPVVAHPECTEAVRLLADHICSTE
                     KMIPYCTENPAKSFIIVTESGILHRLRKLVPGKEFIPAPTEQCSCSTCPYMKMNTLEK
                     LYLALRDLSPAVELPEDLRRRAEAPLLRMLEQSQS"
     gene            7832..9271
                     /gene="ffh"
                     /locus_tag="PK077_03135"
     CDS             7832..9271
                     /gene="ffh"
                     /locus_tag="PK077_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRN23325.1"
                     /translation="MFSLLADKLDNTFRRLRGLGKISEKNIADALRDIRLALLEADVE
                     FGVAREFIGRVKERAMGQEVLKSIKPGEQIVKIFRDEIAALLGGDAVGLDLTDPARIM
                     VVGLNGAGKTTTSAKLALRLKNEGRRPLLVACDLVRPAAVDQLATLAEQIGVPVYKPA
                     PGSRDVVAVAREALEWARTQNGTVMIFDTAGRQEVDEALIDELRHLHSFLAPRETLLV
                     ADAATGQQAVNVAQTFDRAVNVTGIVLTKLDGDARGGAALSMRSVTGKPIKFSGEGEK
                     LDQFGPFVPGRMADRILGMGDIVGLVEHAAARIDEEQAAKSMDRMMSGKFDFNDFLDQ
                     MKMMQSLGPLEGLLGLLPGFGKIKKQLPEGALDPRRMKHMEAIVLSMTAKERSNPNLL
                     NPSRRRRIAAGCGRPLMEVNKLIKNFSEMRKMMSGKGKMGAMMKQMASMKGKGGKVPG
                     FPGMGGGLGGLKGLGGLGGGLGGLFGGRR"
     gene            9400..11049
                     /locus_tag="PK077_03140"
     CDS             9400..11049
                     /locus_tag="PK077_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRN23326.1"
                     /translation="MMSKGRCFRLAAWLALSGVPGTGMAETLPAEEVLLPAPSACRSD
                     RSSVAHLPQRLAVYAPEKDVPVVEALLNVLNAQGVLSGLSMTRDKTSADAVCSVEGSP
                     SGTGEGYEARLVRDREGRGILHLKAAAAQGLFYAWQSMVQVLNANRTGEGFSVPVFSL
                     RDVPRFEWRGMMLDVSRHFFTVAEVKRMVDAMSLFKLNRLHLHLTDGPGWRLEIKKYP
                     LLTSMSAWRVPLADGEWNWREVKLAIREHDPEATYGGFYTQEQMREIIAYARSRQVTV
                     VPEIDLPGHSYAAMHAYGDLICDGVDFPVEGKQGRDSVCMGRPETLRFVKDVVDELKA
                     VFPPGSPIHLGHDEVSAEAWKKCRYCQNRLKSLREKDLKALSRDFLQRIADYVRKGGY
                     EAIVWDEAGELEADKRILVMAWRGNGFAAAAARKGHPVILAPCSHFYFDYYQSAASTE
                     PKAVGGLIPLKQVYGYELPELSPEEAARVRGLQANIWTEYLYNMGLVEYMAWPRALAL
                     SERAWSDCDPRDYKSFRSRASRALNILEKLAVKYRPMGEED"
     gene            11185..11943
                     /locus_tag="PK077_03145"
     CDS             11185..11943
                     /locus_tag="PK077_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HRN23327.1"
                     /translation="MKKHRFLFSASLMALVLLGCMAVVSCSNNFKHVPQTGFITHQVK
                     NKDSHIPFDAYWNADDKKIWNDRVNGVDGKKVMMAVVPVDTEHMDVRPDTAEGFDALR
                     GLAEYFRKSVATRMEKEVAENPHFEMVPPGTRGAYTLELAIISITPTNARAGIFVTAL
                     SGIKGGGLVKRFIKKGHIAMAGRLRDESGRIVSEFADYEEDHSSLLGVDTKDFKKYAH
                     HQRTIDEWAREITDVYSSAYGHWTQKRMISLNPF"
     gene            complement(12041..>12227)
                     /locus_tag="PK077_03150"
     CDS             complement(12041..>12227)
                     /locus_tag="PK077_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRN23328.1"
                     /translation="IIPFDREDPTSEVMARVIFNVARHALEKARENPGEYPVRACVEL
                     ERVRVWETSSSWAEYGE"
BASE COUNT         2445 a         3372 c         3767 g         2593 t
ORIGIN      
        1 gggggtggcc ttcctccgcc gcctgcctgt accatacgcg catattttcc cgcgcttcat
       61 gggccttccc tacggaatcc agtgtattgg ccagttcctc cttccacagc tcgcccaact
      121 gtacgctgga atctgtgtcc ccaagctgag aggccagctc aagctcctta tgtgtcttgg
      181 cgtagggggg aagctggttg agttttttct gggggaagga aagaggttcc ttcaggtatg
      241 gcatgccccg ttcgtcatga agggacagca gatcaagggc cgccttgcgc agctcctgtt
      301 cctgcggggg ctgcgcttcg gaaattaact gggcgtacca ggcacaggcg ttattgctgc
      361 ccagtttcct ggcctgctcg aacaaaggct ggaggaggga gcttttccct ccccctgttt
      421 tttccttcat gccggcggag aggtccgcca gcagagtttc cagatcccct ctggagcgca
      481 gttccgccaa aagctttccg gcctgtgccg gtgcggcccc cttcgtcaat tccgccagaa
      541 gccgcatggc ccccgcgtgc cccttttccg cagcccggga aagggattga atggccgtat
      601 ccctccatcc cctgccgtcc ttgccgtcca gggcaagggc cagttcccct atccgcgcca
      661 gaagaaccgg tttggcgttt ttccggtatt gcttcagggt ctcgataacg ttggcgggat
      721 tttccttcag ccaatccact ttgatgaaag cgagctgggc cagcgcatcc tggtcgccgg
      781 aagcggcctt cttttccagg ggggccaggt agcgtctcaa ctcggcttcc ggaagatccg
      841 tgacggtgta cagggccgta acggcatcca tgtctccctt ttccgcaagc ttgcggcagg
      901 cagccagcca ttccgcatgt tccggttgca tgggttgcgc cttgaaaatc ccgaaaatcc
      961 cgtaaggatc cgtcatcatg agcttgggtg ccggatggcc ggatgccgtg gccgcatcac
     1021 ggaaaaaatg ctgcatatgt tccaggcagg ccagcggcag gtctccacgg ttcaggtaat
     1081 aaagccccat ccagtattcc ccggccagca tattggcccg gaagtctcca gccgcggctt
     1141 tgtccagcac ttccccgtac gtccagccgc cgtccatagg cagttgggag aggtcccgga
     1201 ccggagcgta tttcacctgc ccgggaggat ttgccgctgc gtcggacgaa atgtcctggc
     1261 cataagccgg aacggaaaaa ataaagggaa taagcaaaag acgggctcgc atgggtcaac
     1321 tcttttctag caagaaaaac ctctgcccta caagcgtttt tcacgtgtct gaatgtttaa
     1381 ttattggtca tgtcacgttc tttttttaaa gaaaaaaaat ggagtgggac cgggtggttc
     1441 cgaagacgcg tgcggaagcc cgcaaggcag aggaaaatgg cttcctggga gacgggatga
     1501 atgaagtggg caagggattg gggatttcaa ccccgccgta tttcttcatt ccatcaggtc
     1561 cggattcttt tgatgaatat ccccggaaga ttacctttta gtaaattccc ttcaactttt
     1621 ccggatgatc gctcaactgg gagcgcagcc ggtttctgtc cagctttccc atgaccgtga
     1681 gggggatggg cgtttccatg acggcgcgga tgcgctgggg gcctggggcc gacgcattcc
     1741 actcgcggca ggcctgcttc aggggggcag cggaagggcc gcaggcgacc agttccattc
     1801 cggcgcgcgg atgtggaact gcctccacga cagcctccgg gacgctgcgg cgtaaaacgt
     1861 cctgaacggc atccggatcc accagttccc ccaggacttt gacaagtctg tccgcccggc
     1921 gcagaaaggt cagcaaacgc ccttccaggc gcaccaggtc gcgggtgctc caccactcct
     1981 gcggagcgac tctttccaga aggaaccctc catgagactg cgtcagcagg cggccgaaca
     2041 atttgccttt cccctggaag cgcaggaggc cgcctttgtc cgtctgagct tcccaatggg
     2101 gcagaaggga aaggcggtcc gtatggaagg aatctccggg aagggccgtg gctaattggg
     2161 agccggcttc cgtcatgccg tagctctgca ctacgggcca gccaagggtg cgtgctttcc
     2221 gtcccgtctc cgtgtccagc gcgccgccgc ctacgataat gcacccggcg gtggaaggcg
     2281 cgcggaggcc gaggctgacc agatccacta cctgcgtggg gaccagggag gaccatctgg
     2341 ctccggaact tttcatcagg tcggcataag cctgcgggca ccactttccg cggagcgtat
     2401 ggacggcggt tccggccagg gccgcgcgca ggtaaatcat ggcgcccccc acatgccaga
     2461 gaggaagcgg gcagcaccag tccccgcttt ccgcatgcag atgttccaat gccccgcgcg
     2521 cgcatatgtc cagggcgcgg cgggtaaaca aaatggcttt gggcggagaa ccggaagagc
     2581 cggaggtggc gaaggctgtg attccgtcca tgccccggag ggaaagaaag gcggcggcct
     2641 cttccgccca gcccctcaaa tggggcgggg ccatcacgca ggggccgttg ccgcggtccc
     2701 aaggcagggg tgttccggtg gcgggcatgg cggcggcagt ttaccggagg gcttttctgc
     2761 gcagggtgtg ccagcgttcc tccatgcggg gcttcaaatg atgctcaatc catgcatagg
     2821 ctgcttccag gccgcccgct ttgaaagtgc ggcctatctc cgcttccacg cctgtggcgt
     2881 tgccggtgcg gcgcagaaaa tcattgctgg tgaattctac gaaaagcttg ccgttgatcg
     2941 ctccttcctc cgccagaatc cattccctga cgtacaacgc caaaatggcg tcccctaccc
     3001 atgcctctgc gcggaggcgt tcttctgtag ccttgtccat atgatggttc atgaaaatct
     3061 ggcaatggcg gttttccttc ccagaggatc cctgatggga ggcggaggca cgccgggggc
     3121 cttgtctcca gagggggaag ccggttggcg gttttcccgc gggagctccg gcaggttgtc
     3181 cgcgggggag gcgggagctc ccggaattgg cggattttcc ttgctgccct gtggcgcagg
     3241 cgcggctccc ggaaccagcg gcacgtccag cactctgtcc tgggccgcgc ggcggttggc
     3301 ggcgtccctg gcttccagag acttgcggag taccgtttcc gattccgaat tccagtaccc
     3361 ggaatccagt tcaatataaa tattggagta aggcagggat gtcttggttt taatgaccag
     3421 aacgttgaat tgcctggccg cgtcttcgat cagggaattg atcgtacggc tcatcagttc
     3481 aaaatgctgg cgcccaggaa gcattttatt gatattgttg cggtagcgct taatgccggg
     3541 agcatagtcc tccgtaatgc tgcgcagctc cgagctgatc atgatgcggg gtaccacgtg
     3601 ggcttcctct tccagcatat tcagcacatc caggagtact tcgtccgacg tcttgtcgga
     3661 aaccatggtt ctgatgccgg ttcccgtgta ggcgggaaag gaggattccg cgatgacaat
     3721 ccagttcctg gcccccacga ttgccaggtt tttatataac tgctccttcc attcccgctc
     3781 tgccgggttg gcgtcctggc agccgctgag caggctgatg atgcatatgg gcaggatgag
     3841 tcgggtcatg ggtgggaccc cgccttgcgg ggctggaaaa tgggctagaa tttatgggat
     3901 cggggttcgc ggcaggcact ggggcagcgg ccttgttccg tagcgaacgg cgctccctgg
     3961 gtgggagccg cctccgtaaa ggcgacgccc ggcagttcag taggtacgcc ttgaacaggg
     4021 aaatcctttc gcagagggaa ataaggataa ccttcccaca tcaaaatgcg gcgcaaatca
     4081 ggatgacctg agaacttgat gcccatcagg tcccatgctt cacgttcgtg ccagttggcg
     4141 gactggaaaa tttccgtgac gctgggcact tcttcgccat cgtccacctt cgtcttgagg
     4201 ctcaggttcg ctcccgtttc cgactgtgtc agcgtgtagt tgacttcaaa gcgggggctt
     4261 tcccccatat tgtccacgga agaaaggtta accagcatat caaacccggc ggaatcccgc
     4321 agccagcgga caacatccgc cacggcttgg cgcgcgacgg tcagcgtaat atctccgcgg
     4381 aattcgtagc ggtccgtgat gatgtctttc ccaaagcggt tttgggcgtt gtctgcggcg
     4441 gttttaatct gtgattctag agaaagcatg gagaaaaaat ggtaaaagtg aacggaagaa
     4501 acgtttgaaa aaacttattc aaggggcatt tccgtattga ccgggacggg agcgttggct
     4561 tcgcgaggtt cttccttctt gaagaaagtg cgtgcgggct gctgggtatc cagcttcttg
     4621 cgcagggtga gcagggcttc cagaatagcc tccggacggg gcgggcaacc ggaaatgtaa
     4681 acgtccacgg gaagaatctt gtccacgcct tggagtacgg aataggagcg gaacatgccg
     4741 ccggtggaag cgcacgcgcc catggcgatg caccacttgg gttccggcat ctggtcccag
     4801 atgcggcgga cggccagggc cattttataa gtgacggtgc cggctacaat catcacgtcc
     4861 gcctggcggg gggaaaaacg tgtcacttcc gagccgaagc gggaaaggtc ataccgggaa
     4921 caggccacgg ccatgaactc aatggcgcag caggaaagac ccatcggcat gggccaaagg
     4981 gaatttttac gaatccagtt gatggcggcg tccagggtgg tatagacaaa attgccttcc
     5041 gcattggagt ccagtactcc cgtttcgtaa atattcggtt cgttcttggt gaccatgata
     5101 acagatgatt cttgctgaag ccagattagc gagatgcctt caactctcaa gccttaattt
     5161 ggttcagcca tgattttctg cggcctgttc cggccctgtt ttccggcatt ccgggagggg
     5221 ggatgaataa acccgcacag ggtttccccc gtgcgggttt tttatccaac tatattacct
     5281 atgaaactca gaactcccca taatgggcgg cggtctctct acctcccgcc gcccggtttg
     5341 ttgctgatgc aggttagagt cccggacagg atcgcccgtt caaaaagaag aaatttttat
     5401 gacaaaacgc caggtatata tttaaatgat tgaaaataaa tatattgtgt gaaaatttat
     5461 tcaaaaaata cggggtagga gttcatttct ttgtgatgcc tgtgaggaaa gtgacggaac
     5521 ggacggagag gccgttacgt ttttcctgtt ccttggcagc tgtgttctcc ctgcgctccc
     5581 gccgtttgcg gattttcttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnc ggaagaatgt ctttccccgc aaaaagagtg cttgtctttt ccgttggatg
     5701 agtcccttgg atttcctccg gttgttccaa acccggcgtt ttggcgtcta agggttattc
     5761 cggctgcagc cgctggcgtt cctgaatgag tacgtggaag tgtcctccgg caccgtattc
     5821 atccgtcaga aaggcatcct gcggcgtgtt ttccgcatgg gggagcaggt agtcccgctg
     5881 ggtcatgaaa gtgacccggt cctccagaca gttgcgggat agttccagca ggtccgtaaa
     5941 attgacgtca caggtcaaat ccgtgaggcc ggggttacgg tacagttcct cccccgtcag
     6001 cacctggtgg cttttgtacc cgcgcaggga accccggggg cggcgatagt acagccgctc
     6061 catttcatcc ccgtagtcga tgaccgtcat gacgcccatg ttccagaggg gaagccagct
     6121 cgtaaaccag cgcgcgtagg agtcgtgaat ttccacccgc tgccccggct gggagccgta
     6181 ttccagcata tgggagaaaa gcggctgctg ccggacgggg cgcagttctt cccggacggc
     6241 tccgtttttc acgacaagcc ctacttcttt ccagtcctgt tccgtgtatt caaatacgcg
     6301 cgccgggaag gcatccacca gctcgttgga gaaaataaag gccttgcctc cgcagtgttt
     6361 cagcgctttt tccatggtgg agtaaatttt agcgcggcct cccagcagaa ggtgctggaa
     6421 ttcgcgcaga cgtggggaag attccacaat cacgtagtcc gtattcaggc ggttccagaa
     6481 tcccagccgc tccaggattt tgacggccag ggcgccggaa ccggcgccta tttccagcag
     6541 gggcagccgc ctgccgcagc gggagcaggc ttctttccag tgagcgacaa tagcttttgc
     6601 gaggatgggg gaaagcgtgg gggtggtgga aaagtccccg cgttgaccga tgttttcaat
     6661 ggaagaggag tagtaccctt cctgcgggtg gtgcagggcc agctgcatga aagcttccag
     6721 ggagagccag ccgccagcgg ccgcgatgtg gtctgaaagg cgtatcatgc aggaaaggca
     6781 ggggtcagga ttgggattgc tccagcatgc gcagcaacgg ggcttccgcc cttctgcgca
     6841 ggtcttccgg cagttccacg gcgggagaaa ggtcccggag ggccaggtac agtttttcca
     6901 gcgtgttcat tttcatgtac gggcaggtgc tgcaggagca ttgctccgtg ggcgcgggaa
     6961 tgaattcttt gcccggcacc agcttgcgga gacggtgcag aatgccggat tccgtcacaa
     7021 tgatgaaaga tttggcgggg ttttccgtgc aataggggat cattttttcc gtggagcaga
     7081 tatggtccgc cagcaggcgc acggcctccg tgcattccgg atgggccacc accggcacgc
     7141 cggggtgctt ttccttcagg gcaagaatct ggtcccgcgt gaactccatg tgcacgtggc
     7201 aggccccgtt ccacagttcc agggggcggc caagctggcg gcttacccaa gcgcccagat
     7261 tctggtccgg gacgaacagg atggggcggt cctgcggagc ctgggagacg atttttacgg
     7321 catttccgga agtaacgatt acatccgcca gcgccttgac gccgatggag cagttcacat
     7381 acgccactac atagtaattt ttatgggcgt tttcttttaa gaaggcttcc agctcaccgg
     7441 cgtcgcagga ggcttccaga gaacagccgg catcggcatc cggaagaatg acggttttgt
     7501 ccggattaag aattttggcg gtttccgcca tgaaatggac gccgcaaaag gcgatcacgt
     7561 cgcaatccgt attctgcgcc ttgtaggcca gccccaggga atcgcccaca tagtcggcca
     7621 tgtcctggat atccccggtc tggtagctgt gcgccagaat gatggcgttg cgttcttctt
     7681 tcaggcgtaa aatttcctct ttcaggtcaa agggtgccat gcgggaaaag atggggcgaa
     7741 gcccccggca agtcaagcat catgtcccat gcagggcgcg cttgttgacg ggaaggggga
     7801 aatgtggcac agtctggcgc atcatacgat tatgttttca cttttagcag ataaactgga
     7861 taacacgttc cggaggctgc gcggcctggg caagatttcc gagaaaaata ttgcagacgc
     7921 cctgcgcgac atccggctgg ccttgctgga ggcggacgtg gagtttggcg tggcccggga
     7981 attcatcggc cgcgtgaagg aacgggccat ggggcaggaa gtgctcaaat ccatcaagcc
     8041 cggagagcaa atcgtcaaaa ttttccgtga tgaaatcgcc gccctgctgg gcggggacgc
     8101 cgtggggctg gacctgacgg atcctgccag aatcatggta gtggggctga acggcgccgg
     8161 caagaccacg accagcgcga agctggccct gcgcctgaag aacgaggggc gccgccccct
     8221 gctggtggcc tgcgacctgg tgcgccccgc cgccgtggac cagctggcca cgctggcgga
     8281 acaaatagga gtgcccgttt ataagcccgc tcccggcagc agggacgtgg tggccgtggc
     8341 acgcgaggct ctggaatggg cccggaccca gaacgggacc gtcatgattt ttgacacggc
     8401 gggccgccag gaagtggatg aagccctgat agacgagctg cgccatctgc actccttcct
     8461 tgcgccgcgg gaaacgctcc tggtggcgga tgccgccacg ggccagcagg ccgtgaatgt
     8521 ggcccagacc tttgaccgtg ccgtgaacgt gacgggtatc gtcctgacca agctggacgg
     8581 cgatgcccgc gggggcgcgg ctctttccat gcgttccgtg acgggcaagc ccatcaaatt
     8641 ttccggtgag ggggaaaaac tggaccagtt cggccccttc gtccccggcc gcatggcgga
     8701 ccgtattctg ggcatgggcg acattgtcgg gctggtggag catgccgccg cgcgcattga
     8761 cgaagagcag gcagccaaat ccatggaccg catgatgtcc ggcaaatttg atttcaacga
     8821 ctttctggat cagatgaaga tgatgcagag cctgggaccg ctggaaggac tgctgggcct
     8881 gctccccggc ttcggcaaaa tcaagaaaca gcttccggaa ggcgctctgg accccaggcg
     8941 gatgaagcac atggaggcca ttgtgctctc catgacggcc aaggaaagga gcaatcccaa
     9001 cctgttgaat ccgtcccgcc gccgccgcat tgccgcgggc tgcggccgcc cgctgatgga
     9061 agtgaacaag ctaatcaaga atttttccga aatgcgcaag atgatgtccg gcaagggcaa
     9121 aatgggcgcc atgatgaagc agatggcttc catgaaaggg aaggggggca aggtgcccgg
     9181 ctttcccggc atgggaggag gtttgggagg tctgaaaggc ctgggtggat taggtggcgg
     9241 tctcggcggc ctttttggcg gacgaaggta aatgtaaaaa aggatggaaa ctggcaggcg
     9301 cggctgaata cggccgcgcc tttgctttcc ggaacacagg ggcctgaggg aaggctcctt
     9361 ccgttgaaag aagagaccag gggaggccgt acttccagca tgatgagtaa ggggcgttgt
     9421 ttccggctgg ccgcatggct ggctctgtcc ggagtgccgg gcacgggcat ggcggaaacg
     9481 ctgccggctg aggaggtgct gctccccgcg ccttccgcat gcaggtctga ccggtcctcc
     9541 gtggctcatc tcccgcagcg gctggcggtc tatgcgccgg aaaaggatgt tcccgtggtg
     9601 gaagccctcc tgaatgtgct caacgcccag ggcgttttgt ccggcctttc catgacccgt
     9661 gacaaaacct ctgcggacgc cgtttgttcg gttgagggaa gcccctcggg gacgggcgaa
     9721 gggtatgagg cccgcctggt gcgcgacagg gaagggaggg gtatcctgca tctgaaagcg
     9781 gcggcggccc agggcctttt ctacgcgtgg cagagcatgg ttcaggtgtt gaacgccaac
     9841 cggaccgggg aggggttttc cgtacccgtt ttttccctcc gggatgttcc ccgctttgaa
     9901 tggcggggca tgatgctgga tgtttcccgc cactttttca ccgtggcgga agtcaagcgc
     9961 atggttgacg ccatgtccct gttcaagctt aaccgcctcc atctgcacct gacggacggc
    10021 cccggctggc gcctggaaat taaaaaatat ccgctgctta cctccatgag cgcctggcgc
    10081 gttccgctcg ctgacggaga gtggaactgg cgggaagtga aactcgccat ccgggagcat
    10141 gacccggaag ctacctacgg cggtttttac acccaggagc agatgaggga gatcattgcc
    10201 tatgcgcgca gccgccaggt caccgttgtt ccggaaatag accttcccgg ccattcctat
    10261 gcggccatgc atgcctacgg ggatttgatt tgcgacggcg tggactttcc cgtggagggg
    10321 aaacaaggcc gggactccgt ctgcatgggg cggccggaaa cgctccgttt cgtgaaagac
    10381 gtggtggatg aacttaaggc cgtctttccg cccggctccc ccattcattt gggccatgac
    10441 gaggtgtctg ccgaggcatg gaaaaaatgc cggtattgcc agaaccgctt aaaaagcctg
    10501 agggagaagg atcttaaagc cctgagccgg gatttcctgc agcggattgc ggactacgtg
    10561 aggaagggcg gctatgaagc cattgtctgg gatgaagccg gggaactgga ggcggacaaa
    10621 cgcatcctcg tcatggcgtg gcgcggcaac ggctttgccg cggcggcggc caggaaaggg
    10681 catcccgtca ttctggcgcc gtgttcccac ttctattttg actattacca gtccgccgcg
    10741 tccacggagc cgaaagccgt agggggcctt attccgctca agcaggtgta cggctatgaa
    10801 ttgccggaac tttccccgga ggaagctgcc agggtgcgcg gcctccaggc gaacatctgg
    10861 acggaatacc tgtacaatat gggtctggtg gaatatatgg cgtggccgcg cgccctggcc
    10921 ctgtccgaac gcgcctggag cgactgcgac ccccgggatt acaaatcctt ccgcagccgc
    10981 gcctccaggg ctctgaatat cctggaaaaa ctggccgtga aataccgccc catgggggaa
    11041 gaagactagg cattcccgct ttggcccctt ccgcacggcg ggaggaccgc cgccccggat
    11101 gttcacccgc aggttttatt gacagaggat gccgggccat gatagaaacg gttctgccaa
    11161 ttaattgata tggaattatt tgatatgaag aaacaccgtt ttttattctc agcttccctg
    11221 atggctctcg tgctgctcgg ctgcatggcg gtagtgtctt gttccaataa tttcaaacat
    11281 gttccccaaa caggatttat cactcatcag gtaaaaaata aggattccca tattcccttt
    11341 gacgcgtact ggaatgccga tgataaaaaa atctggaatg acagggtgaa cggcgtggat
    11401 ggcaaaaaag tcatgatggc cgttgttccg gtggatacgg agcacatgga tgtaaggccg
    11461 gataccgcgg aggggtttga tgccctgcgc ggcctggcgg agtacttccg gaagagcgtt
    11521 gcaaccagaa tggaaaagga ggttgccgaa aatccgcatt ttgaaatggt gcctccggga
    11581 accagggggg cctatacgct ggaactcgcc atcatttcca tcactcccac gaatgccagg
    11641 gccggcatct ttgtcacggc gctgagcggc atcaaggggg gagggctggt caaacggttc
    11701 atcaagaaag ggcacattgc catggcgggc cgcctgaggg atgagtccgg ccgcatcgtt
    11761 tccgaatttg cggattatga ggaagaccac agctccctgc tgggcgtgga caccaaggat
    11821 tttaaaaaat acgcccacca tcagcgcact attgacgaat gggcccggga aattacggat
    11881 gtatattctt ccgcctacgg gcactggacg cagaaacgca tgatttccct gaatcccttt
    11941 taagggatga acctgcgggg gggggcgttg cggccgtgct ttccacgggg ccgggaatct
    12001 ttgcggagcg ttccgcccgg cgggaaacgc gctgtttcag ttattccccg tattccgccc
    12061 aggaactgct ggtttcccac acgcgcacgc gttccagctc aacacaggcc cgcacaggat
    12121 attcgccggg gttctccctg gctttttcca gcgcgtgccg ggcaacattg aaaatgacgc
    12181 gagccatcac ctcgctggtg gggtcttccc ggtcgaaggg aatgatg
//
LOCUS       DAOMSU010000041        12105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_269_length_12105_cov_3.128050, whole genome shotgun sequence.
ACCESSION   DAOMSU010000041 DAOMSU010000000
VERSION     DAOMSU010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_269_length_12105_cov_3.128050"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2252
                     /locus_tag="PK077_03155"
     CDS             <1..2252
                     /locus_tag="PK077_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23329.1"
                     /translation="TRSSCIPMNPVNPLRPGCPKHKKSSSGGIIMTIVLVLALAAGGV
                     AYHRVSVQRKAAAAELAARKEAEAKAARVRKAAELEAARRKAEERARAEAEEKARLEA
                     ERLAAEEAAGKRRNKEQEVAEPSPEEPKEEPVVQEEDPRLEIFKKPVLLVSLKANNAA
                     NRKLFTDAFNLALETGRYDLYADFLRSNLERDAVKVIKFGKFDASMYDQSPYLMRANE
                     LYQLISKVGAETIQEQIKESSPRYFYPWLFSDPSDPLRLFLRTMAREQTPGEEWGGIL
                     RKWAEFWMKTSAMPRSRYSSLALACAMLNPRIASSPSKLRASSSTNISTTPLTLEQVF
                     EYFMEMDEARELLTDISKLSPSELLFVVDVRLPRSEMDWARKKVRLTRKGWGGAYSMI
                     RYRMDRAALGKDPYTNYTFQEILDEGGICMDQAYFAVNTAKCNGIPSAYVTGDGNRGP
                     HAWVNLLTTDETWQSYGGYGYNTGHFSHPHNCKSKHESTLLQGMDKKVNGARLDTSLD
                     YLSLADLFEEMQKPDCARVMLEAATQATPGSPLGWERLIALMGRPESGTKLEEWDELV
                     SMIKRKFRSRPDYLAMAARVEDEYIFPMRDASTNKRHVARDLKKLEKETDEGRSDLTS
                     AAIKRQADLLMENGDKAGVAALYRKSMKDYARRAEVFEALMGQYYEYVSQDQDAVLQL
                     AKEAESSYNRYIRTKSGDYFKVKKEAAIQRRIAGYYEKGGNGKKADSLRKDAEKREKN
                     SKKGIREEN"
     gene            2382..2909
                     /locus_tag="PK077_03160"
     CDS             2382..2909
                     /locus_tag="PK077_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HRN23330.1"
                     /translation="MAREPYANFWPKVADTAFVAESADLIGDVTIGEHASVWYHTTLR
                     ADINKIVIGDYSNIQDNSCIHLADDFGCYVGKYVTVGHGVILHACTVEDNCLIGMGSI
                     ILDGAVIGQGSVVGAGALITKGTVIPPNSLVLGSPAKVVKDLGPESADNNHKWAEKYV
                     KVAARYTERVINPGF"
     gene            2929..3414
                     /locus_tag="PK077_03165"
     CDS             2929..3414
                     /locus_tag="PK077_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HRN23331.1"
                     /translation="MPDEHLIALEPYALLYTRAGAGVCLRDPLTGKVGVIFMELTDGM
                     ALERCLNGERPVRPDTSSLLAHFLSAMECRVRLVLINGRKDEVFYARVTIEAANEVMD
                     KLVELDARPSDALMMAVRFGTAIKIVPSVWESMENILPQLEQLDADSPDSRAPVYVGD
                     S"
     gene            3573..3658
                     /locus_tag="PK077_03170"
     tRNA            3573..3658
                     /locus_tag="PK077_03170"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3607..3609,aa:Tyr,seq:gta)
     gene            3898..5763
                     /locus_tag="PK077_03175"
     CDS             3898..5763
                     /locus_tag="PK077_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRN23332.1"
                     /translation="MLTTLLILGVSAVLFVQGRIRSDLVALCSLLCLIMFGILSPEQA
                     LSGFSNSIVVMMVGLFVVGGAIFRTGLAKMMGGRIMKLAGRSELRLLVLTMLVTAGIG
                     AFVSNTGTVALMLPILVSLAADGGIQTSRLLMPMAFASSMGGMLTLIGTPPNLVVNNQ
                     LQEAGMEGLGFFAFTPIGLVCIVTGILVLIPLSRKFLGRHDDRAEHEAKGKSLSQLID
                     EYQIINNLYRLRVLEDSPLTEHPLHDLQIPDLYRVNIVEVRRRHSGRHSFLQTVSQTM
                     AGSDTRVARGDVIYVMGEFEDVARFAREYGAEMINRKTSEDTDSLLKSKLKFDEIGIA
                     EMLLMRNSDLINMPVKSSGLRAKYGVNILAIQRDNHYIFHNLKDVKLQYGDTLLVQGT
                     WTDIARLSKKTFEWVVVGQPLAEASKVTLTHKAPLAAGIMLLMIAAMVFNWVPAVAAV
                     LIAAVLMVLCGCLRNVEEAYRTINWESIVLIAAMLPMSVALEKTGASEAISHGLVAGL
                     GGFGPFALMAGVYFTASLLTMFISNTATAVLLAPIALQSAASMGISPYPLLLAVSVGT
                     SMCFASPFSTPPNALVMPAGKYTFMDYVKVGVPLQVIMGLVMVVVIPLFFPFGKA"
     gene            5887..6120
                     /locus_tag="PK077_03180"
     CDS             5887..6120
                     /locus_tag="PK077_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23333.1"
                     /translation="MKYLLIVLAVAFAMPSMAEEDAPKRMTSAEKKAALLEKYDINKD
                     GKLDSEEKAKMKEDLKKQREEERAKRHASRKAD"
     gene            complement(6287..7318)
                     /locus_tag="PK077_03185"
     CDS             complement(6287..7318)
                     /locus_tag="PK077_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HRN23334.1"
                     /translation="MTDSPRPTRIKVIRKSMKASPDQTESLLWGDCLFCTDAAMTDYD
                     WVLVYDEFPRTPVGTIRNETEPLLCPPDQTILITVEPPSIKIYSRAYTSQFGTVLTTH
                     SARDLPHPGHTLGRGCLEWLYIKPMQEILDQKEFPKTKMLSTICSAKQHTHTMHKKRY
                     DLTRYLADRLPELDWFGHGIREIENKTVAMDDYKYHLCVENHLEPHHWTEKLSDAFVA
                     MTLPFYAGDPLATECFPQESFIPIPLDNPQKALEIIRKAMEDGEYEKRLPAIREARRL
                     VLEKYNMFAQTAAVIHNHRETGTIRPGAMLKGRHVLRKNPLNALRELADTLAYKIRSF
                     GRRGTGPGV"
     gene            complement(7582..9549)
                     /locus_tag="PK077_03190"
     CDS             complement(7582..9549)
                     /locus_tag="PK077_03190"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRN23335.1"
                     /translation="MSKCFSETFCSPSLHQAGSAIHGTYFPHIDGLRTFAVLAVVLYH
                     LQEGICPGGYTGVDIFFVISGYLIGGGLIRGLKEGTFSLTSFYLRRIRRIMPAYFCLI
                     AVVLAFGCVVLDCDDLRTLGRTVRSSALFITNIYFSRTTGDYFSPAAEENPLLNLWSL
                     SVEEQFYLMIPLTLWLLWKFREKAVKPVLWGALGLSFFAAVCHMGHLEHNKAFYLLYC
                     RAWELLAGCLLALAPAAGQNRGAGWLRAAGWAGILLPFACYTPSTPFPGYTAIPSVAG
                     AALLIRYGNHGWSGRILRHPLSTGIGKISYSLYLWHWPVIVYWTYICFDECAPWDYAG
                     MFLLSLLLGFLSWKFVETPFRTTVSWQNPQKAFLATAAGCLMLGLAGEWLKWTDGARD
                     YWHVAANNMEFPEYWKGPAFPPSFGITPPDRAVVEKGNAIQHHHPELGDVTDYPFVLL
                     GKTGQAPSFLLMGDSHAMASSPGFDEAARLLQRSGLFYRARLCPLSGISSSGDSSSLT
                     LLRLMYPHWEHNMELILDWLENTPQVQTVFIHNRWIELVNNHRDTRLKQLVADGLRHT
                     CARLRKAGKQIVLLGPVPEWTFGPRKLMRRNALLNANRTDRLLGGDFNTRQRPDSPCW
                     STWNPQACAASFPSTAPSIKMGGGRRKTAAN"
     gene            complement(9633..10631)
                     /locus_tag="PK077_03195"
     CDS             complement(9633..10631)
                     /locus_tag="PK077_03195"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HRN23336.1"
                     /translation="MKTLKISFLQSTPDFGREGMLQLLKSRYHVVEDDSYFDYLVATP
                     WFYVNREAFYDFLERAPGHITVMYGCHEAIAPDFMLFDYYIGLDTVPGSDRTVKLPYL
                     RHHLQEVHGGKEGLDAHALLASKTGFCNFIYANRKSHPNRDAMFHKLSAFRFVNSLGP
                     HLNNTPGDGHRAEDWYASSIRMKKPYKFSIAFENAWYPGYTSEKIVTSMLAGTIPIYW
                     GNPDISREFNSASFINCHDFPTLDDAAAYVQKVDEDDNLWCEIMSRPWKTPEQEARFL
                     EETERETVKLYKIFDQSPEEARRKGDGTWVSYYQRFLKRGHRMQLAWRRLKNRLRR"
     gene            complement(10752..11954)
                     /locus_tag="PK077_03200"
     CDS             complement(10752..11954)
                     /locus_tag="PK077_03200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN23337.1"
                     /translation="MSDALNRHIPGLKSTLLLRRRKKIDGLEIHRTPARRATDALRRH
                     LAHGFDILLPERRKDLPFSLNVLGMGFDTAQMEKADVVHLHWIGGRTVDFSQLRHIRR
                     PLVYTLHDMFACTAGCHCTMDCSLFQDECRGNCPQLGAPRLFRNIPAWLFSRKRRAFG
                     RIPSLTLVTPSLWLKREVERSCMFPGRKIVQIYNPVDTDLFRPAEDRNAIRAGLGIPP
                     GAFVIACGATGLFNPVKGGHFIPLVLEELYRRGHRNLHLLLFGNQEKSVDYPFPSTSA
                     GFITDMNKLAGLYSAADIFLNPTLQDVLSNVALESMACKTPSVTFRTGGVPEAVLDGR
                     TGLVAPQGDLNQMAAHCERLIQDGKLLQTLAEEGRRHAEQTFSYPVIAARHAALYEET
                     LREYRYEE"
BASE COUNT         2549 a         3074 c         3756 g         2726 t
ORIGIN      
        1 caacgcgttc aagctgcatc cccatgaatc ctgtcaatcc tctgcgtccc ggttgtccca
       61 agcataaaaa atcctcttcc ggcggaatta ttatgactat tgtcctggtg ctggctcttg
      121 ccgccggagg ggtggcttac caccgggttt ccgttcaacg taaggctgct gccgctgagc
      181 tggcggccag aaaggaagcg gaagcgaaag cggcgcgtgt acggaaagcc gcggaactgg
      241 aggcggcgcg caggaaggcg gaggaacggg cgcgcgcgga ggcggaggaa aaagcccgtc
      301 ttgaggcgga acgcctggct gctgaggaag ccgccggaaa acggcggaac aaggaacagg
      361 aagtggcgga gccttcccct gaggaaccga aggaagaacc cgtggtccag gaggaagatc
      421 cgcggctgga gattttcaag aagcctgttc ttctggtcag cctgaaggcc aataacgccg
      481 ccaacagaaa gttgtttacg gatgctttca atctggccct ggaaacgggc cgctatgact
      541 tgtatgccga tttcctgcgc tccaatctgg aacgggatgc cgtgaaggtc atcaaattcg
      601 gcaaatttga cgcgtccatg tacgatcaga gcccgtacct catgcgcgcc aatgagctgt
      661 accagctcat cagcaaagtg ggagcggaaa ctatccagga acaaatcaag gagagttccc
      721 ccaggtattt ctatccatgg cttttttcag acccttccga tcccctgaga cttttcctgc
      781 gcacgatggc gcgggagcag acacccgggg aggagtgggg agggattttg aggaagtggg
      841 cggaattctg gatgaaaacc tccgccatgc cccggagcag gtattcctcc ctggcgctgg
      901 cctgcgccat gctgaatccc cgcatcgctt ccagcccgtc caagctgagg gcgtcttctt
      961 ccaccaatat ttccaccacg cctttgacgc tggaacaggt attcgagtac ttcatggaga
     1021 tggatgaagc ccgtgagctt ctcacggaca tttccaaatt aagcccttcc gagctgttgt
     1081 ttgtggtgga tgtgcgtctg ccgaggtcgg agatggattg ggcgcgcaaa aaggtgcgcc
     1141 ttacccggaa aggatggggc ggagcctatt ccatgatccg ttaccggatg gaccgcgccg
     1201 cactgggcaa ggacccttat actaattata ccttccagga gattctggat gaaggcggca
     1261 tttgcatgga ccaggcctat tttgcggtga ataccgccaa atgcaacggc attccctccg
     1321 cctatgttac cggggacggc aaccgcgggc cgcatgcctg ggtcaatctg ctgacgacgg
     1381 atgaaacctg gcagtcctac ggcggttacg gatataatac ggggcatttt tcccatccgc
     1441 acaactgcaa gtcaaagcat gaatccaccc ttctccaggg tatggacaag aaggtgaatg
     1501 gcgcccggct ggatacttcc ctggattatc tttccctggc ggacctgttt gaggagatgc
     1561 agaagccgga ttgcgcccgg gtaatgctgg aagccgccac acaggccact cccggcagcc
     1621 ccctgggctg ggaacgcctg attgcgctga tgggacgccc ggagagcggc acgaagctgg
     1681 aagaatggga tgaattggtg tccatgatta agcgcaagtt ccgttcccgt ccggattacc
     1741 tggccatggc cgcccgcgtg gaagatgagt atattttccc catgagggac gcttccacca
     1801 ataagcgcca tgttgcccgc gatttgaaga agctggaaaa ggagacggac gaggggcgca
     1861 gcgacctgac ttccgccgcc atcaagcggc aggcggatct cctgatggaa aacggggaca
     1921 aggccggggt ggctgccctg taccggaagt ccatgaagga ttatgctcgc cgcgcggaag
     1981 tatttgaagc gctgatgggg caatattacg agtacgtttc ccaggatcag gatgccgtgc
     2041 ttcagctggc caaggaggcg gagtcttcct acaaccggta tatccggact aaatcgggcg
     2101 attatttcaa ggtaaaaaaa gaagccgcca tccagcggag gatagccggg tattatgaaa
     2161 aggggggtaa tgggaaaaaa gccgactccc tgcgcaagga tgcggagaag cgggagaaga
     2221 acagcaagaa gggcatccgg gaagaaaatt agtgcggagg tgcctgcggc ctttcatttt
     2281 cgggtgggtt cggccgtatt cgtttccggc tgcggcatac ccgcggaatg aacgttttta
     2341 gactggaatg ctgtcttatc ttcaattaat acatgactta tatggcaaga gaaccttacg
     2401 cgaacttctg gcccaaagtg gcggataccg cttttgtggc ggaaagcgct gatctgattg
     2461 gagatgttac cattggggaa catgccagcg tctggtacca taccacgctg cgcgcagata
     2521 tcaataagat cgtcattggt gattactcca atatccagga caacagctgc atccacctgg
     2581 cagacgattt cggctgctat gtaggcaagt acgtaaccgt ggggcacggc gtgattctgc
     2641 acgcctgcac ggtggaggat aattgcctga taggaatggg gtccattatt ctggatggag
     2701 cggtcatcgg ccaggggtct gtggtgggag cgggcgcgct cattaccaaa ggtaccgtca
     2761 ttcctcccaa ttccctggtg ctgggttctc ctgccaaggt ggtgaaggac ctggggccgg
     2821 aatccgcgga taataaccac aaatgggcgg aaaaatacgt caaggtggct gcccgctata
     2881 cggaacgggt tattaatccc ggtttttaac atgtgatttc cgcccgccat gccggatgaa
     2941 catttgattg ctctggaacc ttacgccctg ctttacaccc gggccggggc gggtgtgtgc
     3001 ctgcgtgatc cgctgaccgg caaggtgggc gtcattttca tggagctgac agacggcatg
     3061 gccctggaac gctgcctgaa cggggaacgc ccggtacggc cggacacgtc ttccctgctg
     3121 gctcactttt tatccgccat ggaatgccgg gtgaggctgg tgcttatcaa cggccgcaag
     3181 gatgaagtct tttatgcccg cgtgaccata gaggctgcca atgaggtgat ggacaagctg
     3241 gtggagctgg acgcccgccc ttcagacgcc ctgatgatgg cagtccggtt tggaaccgca
     3301 atcaagattg ttccctccgt atgggagagc atggagaaca ttctgcccca actggagcaa
     3361 ctggatgcgg attccccgga cagccgcgct ccggtatatg tgggagattc gtaaaaattg
     3421 ccttttccga ctttttgttc ctgcatccgc tttccttccg gcggcatggc tgttgcatat
     3481 aggggttctg cttccgggag gacctacttt ttagaaaaaa aacttgctaa ggcacgggaa
     3541 tccttataca ttcgccgcac atcacttcaa gtgggtagat tcccgagcgg tcaaaggggg
     3601 cagactgtaa atctgttagc atttgctttc gaaggttcga atccttctct acccaccact
     3661 tttcatttta tggcgcgttc tttcagagag cgcgtttttt attgtttcat catccccatg
     3721 ataccgggaa aggaaagagg aagcggggtt ctccgagcag gatgcacaaa gtgttttttt
     3781 ctgccggatg ccggcggaga gacggttttg tgaatgactt gttcagccta tcgacaagac
     3841 atgccggtca tgttagatat cctcccttgc catttttcca ctcctgccgt ttttgctatg
     3901 ttgaccacgc ttctcattct gggagtatcc gccgttttat ttgtccaggg gaggattcgt
     3961 tctgatcttg tcgcgctgtg ttccctgctc tgcctgatta tgttcgggat tttgagcccg
     4021 gaacaggcgc tttccggctt ttccaattcc attgtggtga tgatggtggg gcttttcgtg
     4081 gtgggaggcg ccattttccg gacgggcctg gccaagatga tgggcggcag aatcatgaaa
     4141 ctggccggca ggagcgagtt gcgcctgctg gtgctgacca tgcttgttac agctggcata
     4201 ggcgcctttg tcagcaatac ggggacggtg gcgctgatgc tgcctattct ggtgagcctg
     4261 gctgcggacg gaggcattca gacgagccgc ctgctcatgc ccatggcatt tgccagcagt
     4321 atgggcggca tgctgacgct gatcggcacg ccccccaacc ttgtcgtcaa caaccagctt
     4381 caggaggccg gaatggaggg gctgggcttt tttgcgttca cccccatcgg ccttgtctgc
     4441 atcgtgacgg gcatcctggt gctgatcccc ctcagcagga aatttctggg acgtcatgac
     4501 gacagggcgg aacacgaggc caaggggaaa tccctcagcc agttgataga tgagtaccag
     4561 attattaata atctttaccg cctccgggtg ttggaggatt cccctctgac ggaacatcct
     4621 ctgcatgatc ttcagatacc ggacctttac cgcgtgaata ttgtggaggt gcgccgcagg
     4681 cattccggcc ggcattcctt cctgcagacg gtcagccaga ctatggcggg ttcagatacg
     4741 agggtggccc ggggcgacgt catttatgta atgggagagt ttgaggacgt ggcccgtttt
     4801 gcccgggaat atggggcgga gatgattaac cggaagacgt cggaagatac ggattccctg
     4861 ttgaaaagca aattgaagtt tgacgagata ggcattgcgg aaatgctgct catgcggaac
     4921 tcggacctta tcaacatgcc cgtgaaatct tcggggctgc gcgccaagta cggggtcaat
     4981 attctggcca tccagcgaga caaccattac attttccata atttgaagga tgtgaagctc
     5041 cagtacggag atacgctact ggttcaggga acctggacgg atattgcgcg cctgagtaag
     5101 aaaacctttg aatgggttgt agtgggccag cctctggcgg aggcctccaa ggtgaccctg
     5161 acgcataagg cccccctggc cgcaggcatt atgctgctga tgattgcggc catggtgttc
     5221 aactgggtgc ctgctgtggc ggcggtattg attgccgccg tcctgatggt gttgtgcggc
     5281 tgtctgcgca atgtggagga ggcgtaccgg acgatcaact gggagagcat cgtgttgatt
     5341 gccgccatgc tgccgatgtc cgtagcgctg gagaagacgg gagcctcaga ggccatttcc
     5401 cacgggctgg tggccggact ggggggattc gggccgtttg ccctgatggc gggcgtctat
     5461 ttcacggcgt ccctgctgac catgtttatc agcaatacgg ctacggcggt gcttctggcg
     5521 cctatcgccc tgcaatccgc ggcgagtatg ggcatcagcc cctatccgct gctgctggcg
     5581 gtttccgtag gcaccagcat gtgttttgcc tctccctttt ccacgccgcc caatgccttg
     5641 gtcatgccgg cggggaaata tacgttcatg gattacgtga aagtgggcgt tcccctgcag
     5701 gtgattatgg ggctggtcat ggtcgtggtg attccgctgt ttttcccgtt cgggaaggct
     5761 tgaccggtgg gagttcatgc ggcggaaaag gggcatggcc gggtaatttg catgtattgc
     5821 taaaaaaaga gagacaaccg gcacgtgcgg gagtagaaaa ggattattca aaaaccccat
     5881 ttaatcatga agtatttgct tattgttctg gccgttgcgt tcgccatgcc ttccatggcg
     5941 gaggaagacg ctcccaagag aatgacttct gcggagaaga aagccgctct ccttgaaaaa
     6001 tatgatatta acaaggatgg aaagctggat tccgaagaga aggccaagat gaaggaagac
     6061 ctgaagaagc agcgggagga ggaacgggcc aagaggcacg cttcccgcaa agcggattaa
     6121 gttttttccg ttttaatggc ctgtttcccg tccggtttga atcggtttca gaccgggctg
     6181 ggccgttccg tctgccgcaa ggcagttggc ggaatattgg ccgcccattc tttttaccgg
     6241 cagggggaat ttgttccctc ctgttggcac gcgcccggaa tccaggttac acgccggggc
     6301 cggttccgcg tctgccgaac gatctgattt tataggccag ggtgtctgcc aattcccgaa
     6361 gggcattgag cggatttttc cgcaggacgt gacgtccttt cagcatggct ccgggccgta
     6421 tggttcccgt ttccctgtgg ttgtgaatga ctgccgctgt ttgagcgaac atgttgtatt
     6481 tttccagcac caggcgcctt gcctcacgga tggcggggag ccgtttttca tattctccgt
     6541 cctccatggc cttgcggatg atttcaaggg ctttttgcgg gttgtccagc gggatgggga
     6601 tgaagctttc ctgcgggaag cattccgttg ccaacggatc cccggcgtaa aagggaaggg
     6661 tcatggcgac aaaggcatca gacagttttt ccgtccaatg gtggggttcc agatggtttt
     6721 ccacgcatag atgatatttg tagtcatcca tggcgacggt tttattttct atttcccgga
     6781 tgccgtgtcc gaaccagtcc agttccggca gacggtccgc caggtaacgc gtaagatcat
     6841 agcgcttttt atgcatggta tgcgtatgct gcttggcgga gcagatggtg gagagcattt
     6901 ttgtcttcgg gaattccttc tggtccagaa tttcctgcat aggcttgatg tacagccatt
     6961 caagacatcc gcggcccagg gtgtgcccgg ggtgcggaag gtcccgggcg gagtgggtgg
     7021 tgagaacggt gccgaattgg ctggtgtatg ccctgctgta gattttgatg gaagggggct
     7081 ccaccgtaat caggatagtc tggtccggag ggcagagaag gggttccgtt tcattcctga
     7141 tggtgcccac gggagttctg ggaaattcgt cgtataccag aacccagtcg taatccgtca
     7201 tggcggcgtc cgtgcagaac aggcagtcgc cccacaacag ggattccgtt tgatccggag
     7261 aggccttcat ggattttctg atgaccttga tgcgtgtggg gcggggggaa tccgtcatgg
     7321 gagtacaggc gttgaagagg ggggcgcggt atggcctcca tattccgggc ttttatccgg
     7381 gaccatcgcg aaggctttgc tgcctggcgc cagtgtaggg ggcctgcatg ccttgtcaat
     7441 gtcaaaggat tggatgtttt tccgtgcggt acttccgtca gttggctgct gttctttcca
     7501 ttcccgggaa aagctggtcg atgatgccgc cgaccatttt ttttgcccca tagggggaaa
     7561 gatggttgtc gtcgcagtac attaattggc cgccgtcttc cgcctgccac cgcccatttt
     7621 tatggaaggt gccgtggagg ggaatgaagc ggcacaggcc tgtggattcc atgtgctcca
     7681 acagggcgaa tccggacgct gccgcgtgtt gaaatctccg cccagcaggc ggtctgtccg
     7741 gttggcgttc agcagggcat tgcggcgcat gagcttgcgc gggccgaaag tccattcggg
     7801 aacggggccc agcagcacga tttgcttgcc ggctttccga aggcgcgcgc aggtgtggcg
     7861 caggccgtct gcaaccagtt gtttcaggcg cgtatcccgg tggttgttga ccagttcaat
     7921 ccagcggttg tggatgaata cggtctgtac ctggggggta ttttccagcc agtccaggat
     7981 tagttccata ttgtgttccc aatgggggta catcaggcgc aggagcgtta gggaagagga
     8041 gtctccggag ctggaaatgc cgctcagcgg acacaggcgc gcgcggtaga acaggcccga
     8101 acgctgaaga agccgggcgg cctcgtcaaa cccgggggaa ctggccatgg catgactgtc
     8161 ccccataagc aggaaggaag gggcctgccc ggtttttccc agcaggacaa aggggtaatc
     8221 cgtcacatcc cccagctccg gatgatggtg ctggatggcg tttccctttt ccactaccgc
     8281 ccggtcggga ggcgtgatgc cgaaggaggg agggaaggcc ggccctttcc agtattcagg
     8341 gaattccatg ttgttggccg ctacatgcca gtaatcccgc gcgccgtccg tccatttgag
     8401 ccattcgccg gcaagtccca gcatgaggca ccctgctgcc gtggccagaa acgctttttg
     8461 gggattttgc catgagacgg tcgtcctgaa gggagtttcc acgaatttcc aggagaggaa
     8521 acccagaaga agggagagaa ggaacattcc cgcataatcc cagggagcgc attcgtcgaa
     8581 gcagatgtac gtccagtaca cgataaccgg ccaatgccac aggtacaggg agtaggagat
     8641 tttgccgatg cctgtgctga gcgggtgcct gaggatacgc ccggaccagc catggttccc
     8701 gtaacggata agcaaagccg cccccgcgac ggaagggatg gccgtgtatc cgggaaaagg
     8761 agtggaaggc gtatagcagg caaatgggag caggatgccg gcccatcccg ccgcccgcag
     8821 ccatccggcg ccccggtttt gtcctgcggc gggggcgagg gccagaagac agccggccag
     8881 gagttcccag gcgcggcagt aaagcaggta gaaggccttg ttatgttcca gatgccccat
     8941 gtggcagacg gcggcgaaga aggagaggcc cagcgccccc catagaacgg gcttgacggc
     9001 tttttccctg aatttccaga gcagccacag ggtgagtgga atcatcaggt aaaattgctc
     9061 ttccacgctc agggaccaca ggttcagcaa agggttttct tccgccgccg ggctgaagta
     9121 atcccctgtg gttctgctga aataaatgtt ggtgatgaaa agggcgctgg atctcaccgt
     9181 ccggcccaga gtccgcaggt catcgcagtc cagcacgacg cagccgaagg ccagcacaac
     9241 cgcaatcaga cagaagtagg cgggcatgat gcgcctgatt ctccggaggt aaaaggaggt
     9301 cagggagaag gtcccctctt tcaggccgcg gataaggcct ccgccaatca gatatccgga
     9361 gatgacaaag aagatgtcca cgcctgtata gccccccgga cagatccctt cctgcaaatg
     9421 gtacaggacg acggccagca cggcaaaggt gcgcaagccg tcaatgtgcg ggaaataggt
     9481 tccgtggatg gcgcttccgg cttgatgtag agaaggtgaa caaaaggttt cagaaaagca
     9541 tttgctcatg aagcggagag agggtagatg ggagtggggg aaggcggatg gaggaaaacc
     9601 ggcatggggt tgaatttgcc cgaaaattcc ggtcagcggc gcaggcggtt tttcagccgc
     9661 cgccatgcca gctgcatcct gtggccgcgt tttaaaaagc gctggtagta ggatacccat
     9721 gttccgtcac ctttgcggcg cgcttcttcc ggagattggt cgaatatttt gtaaagtttg
     9781 accgtttccc tttccgtctc ttccagaaag cgggcttcct gttccggggt tttccagggg
     9841 cgcgacatga tttcacacca caggttgtcg tcttcgtcca ctttttgcac ataggccgcg
     9901 gcgtcgtcca gggttgggaa gtcgtggcag ttgatgaatg aggcggaatt gaattcccgg
     9961 ctgatgtccg gatttcccca gtaaatggga atggtgcccg caagcatgct ggtgacgatt
    10021 ttttcactgg tgtagccggg gtaccatgca ttttcaaagg caatggagaa tttatacggc
    10081 tttttcattc tgatggagga tgcgtaccag tcttccgccc ggtggccgtc gcccggggtg
    10141 ttgttaagat ggggccccag ggaattgacg aaccggaaag cggagagctt gtggaacatg
    10201 gcatcccggt tgggatggga tttgcggttg gcgtaaatga agttgcagaa acccgttttg
    10261 gaggccagga gagcatgggc atccaggcct tcctttccgc cgtgcacttc ctgcaaatgg
    10321 tggcgaaggt agggaagttt gacagtgcgg tcgcttccgg gcaccgtatc gagcccgatg
    10381 taatagtcaa acagcatgaa atccggcgca atggcttcat gacagccgta catgacggtg
    10441 atgtggccgg gggcccgttc cagaaaatcg taaaaagcct cccggttgac gtaaaaccag
    10501 ggggtggcga ccaggtaatc aaaataggaa tcgtcttcca ccacatgata ccggcttttc
    10561 agaagctgaa gcattccttc cctgccgaaa tccggagtgg actgtaaaaa ggagatttta
    10621 agcgttttca tggggagaga agggttgaaa cggtgcagag tgtatgaggc gtctttttct
    10681 tgtcaatgac aaaggaagat gcgcccggat gtacaggaac aggcagtccg tcagttgaac
    10741 gccggccgtt ttcactcttc gtaacggtat tcccggagcg tttcctcata cagggcggcg
    10801 tgccgtgcgg caatgacggg gtaggagaat gtttgttccg cgtgccggcg gccttcctcc
    10861 gccagggttt gcaggagttt tccgtcctgg atgagccgtt cgcagtgggc ggccatttgg
    10921 ttcaggtctc cctgcggagc gaccagcccc gtccttccgt ccagaaccgc ttccggaact
    10981 cctcccgtcc ggaaggtaac ggaaggggtt ttgcaggcca tggattccag cgctacgttg
    11041 gaaaggacat cctgaagcgt ggggttcagg aaaatgtccg ccgcgctgta aagcccggcc
    11101 agcttgttca tgtccgtaat gaatccggcg cttgtgctgg ggaaggggta gtccacgctt
    11161 ttttcctggt tgccgaagag caggagatga agattccggt gcccgcgccg gtatagttcc
    11221 tccagcacca gggggatgaa atggccgcct ttgaccggat tgaacaggcc ggttgcgccg
    11281 caggcgatga cgaacgctcc gggcgggatg cccaggccgg cccggatggc attccggtct
    11341 tccgccggac ggaaaaggtc cgtgtccaca ggattgtaaa tctgtacgat ttttcttcca
    11401 gggaacatgc agcttctttc cacttccctt tttaaccaga gggagggagt tacgagggta
    11461 agggacggga tgcggccgaa ggcgcggcgt ttgcgggaaa acagccacgc tggaatgttg
    11521 cggaagaggc ggggagcgcc aagctggggg cagttgccgc ggcattcgtc ttggaacagg
    11581 ctgcagtcca tggtgcagtg acatcccgcg gtgcaggcaa acatgtcgtg cagggtatag
    11641 accaatggac ggcggatatg gcgcagctgc gagaaatcca cggttctgcc gcctatccag
    11701 tgcagatgca ccacatcggc tttttccatt tgggccgtgt caaatcccat gcccagcacg
    11761 ttcagggaga aaggcaggtc ttttcttctt tccggcagca gaatgtcaaa tccatgggcc
    11821 agatggcgtc gcaaggcgtc cgtagccctt ctggcgggtg tcctgtggat ttccaggccg
    11881 tcaatttttt tgcgcctccg gagcagcagg gtggatttca ggccgggaat atgccggttc
    11941 agagcgtcgc tcaaccggta aggggcggag ctggccgaga cgctatggga gacttgaaat
    12001 attgttcggc attgaggaga agccacatag gaaagtatga ggattaggaa tccgccgtca
    12061 agcctacaaa tctgaaagaa aaacgttttg gggaaatcca ggttg
//
LOCUS       DAOMSU010000042        11773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_288_length_11773_cov_2.760625, whole genome shotgun sequence.
ACCESSION   DAOMSU010000042 DAOMSU010000000
VERSION     DAOMSU010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_288_length_11773_cov_2.760625"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..510)
                     /locus_tag="PK077_03205"
     CDS             complement(211..510)
                     /locus_tag="PK077_03205"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN23338.1"
                     /translation="MNSSTVVTAWDGDRLAGLVRVLDDSEMVAYMHYVLVHPAYQGRG
                     IAGKMVEMVKEKYRDYLYLEIMPEESRNAAFYEKHGFRVMEDGVAMQLCNFSNRS"
     gene            1085..2494
                     /locus_tag="PK077_03210"
     CDS             1085..2494
                     /locus_tag="PK077_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HRN23339.1"
                     /translation="MLDALQNAFFQTGDTLTSWLSAFSSFLWGWPLLIMLLGTHLYLT
                     AILRFPQRYLLKALKLYFVKDHTDKGDISPFSSLMVALAANIGAGNIIGVGVAIAAGG
                     PGAVFWCWLTGVLGMATRYSEGLLAIKYRVENKDGNMSGGPMFVLERGLNSKWLGTAF
                     AVFTAIAAFGIGNLTQGNAAAEQLHHAFSIPFWGTAAVLTALTALVMLGGIQGIARVC
                     AFFVPFMAVIYIMGCLYILTIQAEYIIPAIRYILDCAFTGEAAAGGAAGAAVMAAMRT
                     GVARGLFSNEAGLGSAAIASAAAQNRNPVRQALISSSGPFWDTVIICALTGIVLTTSI
                     IAHPDISCTDGPRLTTLAFSKIPYIGSPLLTLSLVTFVVSTILGWSYFGEKALEYLGG
                     IRLIMPYRVIWVAAVFIGCVSKIELVWIFADCANGLMSLPNLISLLALSGVLVQQTRY
                     YLWQHRLDDYDESHIPEGK"
     gene            2582..2657
                     /locus_tag="PK077_03215"
     tRNA            2582..2657
                     /locus_tag="PK077_03215"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2616..2618,aa:Glu,seq:ttc)
     gene            2898..3974
                     /gene="prfA"
                     /locus_tag="PK077_03220"
     CDS             2898..3974
                     /gene="prfA"
                     /locus_tag="PK077_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRN23340.1"
                     /translation="MDYTPLIEKRRQRLEELETVIAEPDFFNDQKKASEIMREHRRLK
                     ELMETWDSLNATEQQLADNQELAKTDDPELAELAALEIPELEAALEKLRSDVQYSLLP
                     RDTTEDRDAIIEIRAGTGGDEASLFAGDLLRMYQRFAEERGWRFEHLESSPSDVGGFK
                     EVVCRIAGEEVFRFLKYEGGVHRVQRVPATETQGRIHTSTATVAVMPEAEEVDIEIRP
                     EDLRIEVCRSGGAGGQHVNKTESAVQIWHIPTGVYVRCEEERSQMKNREKGMKILRAK
                     LFEAKKREEAEKYSAARRNLIGSGGREEKIRTYNFPQNRLTDHRIGYTSHNLDGILMG
                     QLEDLIMALQHAEMQERLAEAGMS"
     gene            3991..4896
                     /gene="prmC"
                     /locus_tag="PK077_03225"
     CDS             3991..4896
                     /gene="prmC"
                     /locus_tag="PK077_03225"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRN23341.1"
                     /translation="MKTLLEVLQAGTDYLARQGCDEARATMQHLLAHVLHCNRTALYS
                     QFDRPVEEAELAPLRELLKRRAAGEPLQHLLGVTEFFRRDFLTDARALIPRPETEELV
                     EMVLKKIPDHPVRILDMGTGSGVIGVTLALELKERAGEVVLADISPQALDLALENAMR
                     LGARVSTIQTNLFANIPQEKTDPHAEDADSAPEGEKEENGRNKLFDVIVANLPYIAEG
                     EKLAPEVMKDPHTALFGGPKGWEIIERFLARARDYLNEDGFVALEIGYDQASVVTQIM
                     DGYGYNYIEVLKDMSGVSRFPFGYR"
     gene            4918..7134
                     /gene="priA"
                     /locus_tag="PK077_03230"
     CDS             4918..7134
                     /gene="priA"
                     /locus_tag="PK077_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRN23342.1"
                     /translation="MQAARILVDGQSDLVLDYGIPPEAGDVKPGCRVQVPLRNRTATG
                     TVLTLSEPAPAWKDRLKPILKLIDPEPLISPVMMNLASWAADYYSVALDQMIRCLLPE
                     TVRQENTAEKMRKMVYLEKTPAREELDALYRKAPRQAQMLDYFSSAKQQSAPLAAFGA
                     GALNVARSLEAKGFISLKEEAVHRDPSTGEQFVPTQPMKLNSQQQKALEEITAMCAAE
                     RKKPVLLQGVTGSGKTEVYLQAVSQIVKSGKSALIMVPEISLTPQTVQRFKSRFAELP
                     SSVAVLHSLLSDGERFDEWHAIRSGKARIVIGPRSAVFAPLQNLGLVIVDEEHDASYK
                     QESSPRYHGRDLAVLRAHLENCAVLLGSATPSLESIHNALIGKYSLVKLTERADGQQL
                     PLIRILDMKTEGRNKSGPNVISERLRMSIDRRLDKGEQVILLLNRRGFARSIQCPDCG
                     HVVTCLHCSLPLTYHRTEDRLMCHLCGFKALPPRSCPECRSANILLQGYGTQKVEELL
                     RRTFPAARITRVDADVARRKNAVRTILNQFRAHKIDILLGTQMIAKGLDFPNVTLVGV
                     LNADLGLHIPDPRAGERTFQLLTQVAGRAGRGDLSGEVIIQTFTPQSPSLQYARHHDT
                     DGFAAQELEMRRTFDLPPFTHIAVLTIRSQHESMAEFATQTLAARLRGMLPPPATMTD
                     PMPAPIPRAHGQFRFQITVKGPSARILSRTLRKLVQEAGLGEDLTAVIDVDAMSFM"
     gene            7194..8018
                     /gene="dapF"
                     /locus_tag="PK077_03235"
     CDS             7194..8018
                     /gene="dapF"
                     /locus_tag="PK077_03235"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HRN23343.1"
                     /translation="MLLHFYKMTGAGNDFVMVDNRDLSLSSVLDKETIEALCDRRFGI
                     GADGLIAVEPSQGKGGIVRMRYYNADGGEAEMCGNGARCFGNFAAALLHHDKTAPLPF
                     ETMAGVVTATFEKDGNVTVNLTNPHSMQLFVLNADPTVGADVHFLNTGVPHAVAFLDK
                     LEDLNISRLGAYLRYHEAFSPKGTNANFARILSPGHIAIRTYERGVEDETLACGTGMT
                     ACALLHAVLADAPSPIQVDVAGGDTLKVGFQRTGDRFTDVTLTGPADLVFTGDIQI"
     gene            complement(8062..8817)
                     /locus_tag="PK077_03240"
     CDS             complement(8062..8817)
                     /locus_tag="PK077_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23344.1"
                     /translation="MLSTVFNSIGCYLLPFGVILLWLLYESKKVKTILLAALVLLSMV
                     CMFFHEAFAPSREYPWDCMVEHIFTGTLLPFLCLLGYLLYVFLCACIPVYRKVWKGIG
                     FFVLGGMSLAFEYVNVFFPVALLAGYLFRFTIFRKFRNHLPLVRKCHGVLAVGVIPGI
                     CLIGVVCAILFPPPESFICDRIHEEMERDMALWNYQSNLVLLSSRFLNMERENGCITV
                     KYYCETNMGPAVYKAFCNNYGGYVRAECSLLNR"
     gene            9458..9532
                     /locus_tag="PK077_03245"
     tRNA            9458..9532
                     /locus_tag="PK077_03245"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9490..9492,aa:His,seq:gtg)
     gene            9582..10253
                     /locus_tag="PK077_03250"
     CDS             9582..10253
                     /locus_tag="PK077_03250"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRN23345.1"
                     /translation="MRNPEFVKAAFSAIAPRYVATNHVLSMGVDLLWRQRVVQLVSEW
                     KPENLLDLATGTGDLALAILNAMPEIRLTGSDFCQPMLDIAARRGLDHLVCADAMNLP
                     FPSASYDAVTVAFGLRNMASYPDALREMGRMLRPGGHLLILDFSLPESLLKSPYRFYL
                     HRILPVIAGWMTGHREAYDYLADSIEAFPCGQAMKALLRECGYSNITAEALNGGIASI
                     YTAQR"
     gene            10250..11755
                     /locus_tag="PK077_03255"
     CDS             10250..11755
                     /locus_tag="PK077_03255"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRN23346.1"
                     /translation="MSAGDAREGIIRGKAARARLGGKLAGLSLPRQIAVIAFWPFLEQ
                     LLSFFVTSSDLFIATKIGVNAQDTINISDGMGAVVFIMWFGFVIQGSIMMGATAIVSR
                     MTGARDYPQAQHGLHQAAMLGLLAGTISCGLLFTCSDFMVTHVLTMNETARAYALQYV
                     YMAAFAAPFSGVVFAINAALRGSGDTRLPFWIMMSVGILNVIFSVIFVFADAPLGGWR
                     IGGIAAGTVCGYGISMCILLFIMLRRRKKIFTGRKNESLEELVRERGEHYAPPLYLSF
                     SHLRPDMGMQKRILKIGLPQAVEVFGMWGIQMFCLSIISELPVKGVLGVHNIAVRIES
                     LSFLPGFAIGMAASTLVGQYLGARNALMARITIWKCMRYAVIFMTGLGVLFSAFPALF
                     MEIFSNGSTTLIDTGIPVLRTMLVVEPFFAACIVMKMSLRGAGDTRRVMLISYGIMGF
                     FRVVCTWVWFRLAPETMTLWGIWLVFAFEMAVQSAILYKIVKGRSWTKLQV"
BASE COUNT         2681 a         3564 c         3082 g         2446 t
ORIGIN      
        1 cccgcggagc caaaatccga aaggattccc gcgggtttac tgtatcctgt ccagctcccc
       61 cgaggcagac cgcagtttct cgactgcgga tttcagccat aaaaaacagc cttccggaac
      121 agtatgctga aaaacggata tatccggcgg acaacggccg catcgcctgc gccccggcag
      181 ccgcagacaa cctctccccg gcgccccaca tcagctcctg tttgaaaagt tgcacagctg
      241 catggctacg ccgtcttcca taacccggaa gccgtgcttc tcataaaaag cggcgtttct
      301 gctctcctcc ggcatgattt caagatacag gtagtccctg tatttttcct taaccatttc
      361 caccattttt ccagcaatcc ccctgccctg ataggcggga tgcaccagaa cataatgcat
      421 gtaggcaacc atctcgctgt cgtccagtac ccgcaccagc cctgccaggc ggtcaccgtc
      481 ccaggccgta acaacagtgg atgaattcat gagagccttg aacagacgcc ccggatattg
      541 tccggaaatc cagccaacgg acaggaacaa ttcttgaaca tcttccctgg aaaatttctt
      601 ctcttccgta aaaacaatca ttttttcttt aattaattat tgattatccg cctgtaccgt
      661 cgcggccaca caaccgcctg agcgggaaac gcctttccct aggccggatt gacaccctgt
      721 ttcaaggttt tatctgctcc cactgccgca gtcaacaccg tcttgtccga gggttttacc
      781 cattccaagg acatggcgga gcctgtgata agaatgcgcg cgccggaaac aggcaaccct
      841 ttccacagtc agcagctcgt ccgcacctgc ctcctccact tccggggaag gaagagccgc
      901 accaggaaca cacatcttct tttcagggaa acgatactcg aatacgggag acggcatttt
      961 tcctagaacc gattccccaa aaacggaaaa ctccgggccc aggttcataa cattgtgaaa
     1021 aacctttctc cgtcatttgt ttaaggactt gtcaccacgc ttcttttccc tagaatcccc
     1081 cggtatgctt gacgccctcc aaaatgcctt tttccagacc ggggacacgc tcacatcctg
     1141 gctgtccgct ttcagcagct ttctgtgggg gtggcccctg ctcatcatgc tgctgggcac
     1201 gcacctgtac ctcaccgcca tcctgcggtt tccgcagcgt tatctgctta aggccctgaa
     1261 attgtatttt gtgaaagatc atacggacaa gggagatatc tccccattca gttccctgat
     1321 ggtggccctg gcagccaaca tcggcgccgg gaatatcatc ggcgtgggcg tggcgattgc
     1381 agccggaggc cccggcgccg tcttttggtg ctggctgacg ggagttctgg gaatggcaac
     1441 ccgttattcg gaaggattac tcgccatcaa ataccgggtg gagaacaagg acggcaatat
     1501 gagcggaggc cccatgttcg tgctggaacg cggtctgaac agcaaatggc tggggacagc
     1561 cttcgccgtc ttcacggcca ttgccgcctt tggaatcggc aatctgacgc aagggaacgc
     1621 agcggcggaa cagctccacc acgccttttc cattcccttc tggggaacag ccgccgttct
     1681 gacggcattg acggctctgg tgatgctggg aggcatccag ggcattgcca gggtatgcgc
     1741 tttcttcgtg ccgttcatgg ccgtcatcta tatcatggga tgcctgtata tcctgaccat
     1801 acaggcagag tatattattc ccgccatccg gtatattctg gactgcgcct tcacgggaga
     1861 agccgccgcc gggggcgcag caggagcggc cgtcatggcg gcaatgagaa ccggtgtggc
     1921 ccggggcctc ttttccaatg aggccggcct gggatccgca gccatcgcct ccgccgcagc
     1981 gcagaaccgc aaccccgtca ggcaggcgct catctcttcc agcggcccct tctgggatac
     2041 cgtcatcatc tgcgccctga cgggaattgt gctgaccacc agcatcatcg cgcatccgga
     2101 tatttcctgc acggacggcc ccaggcttac tacgctggca ttcagcaaaa ttccttacat
     2161 aggctcccct ctgctcacgc tcagcctggt tacgtttgta gtttccacca ttctgggctg
     2221 gtcctacttt ggagaaaaag cgctggaata cctgggaggc atccggctga tcatgcctta
     2281 ccgcgtcatc tgggtggcgg cggtcttcat cggctgcgtc tctaaaatag aactggtatg
     2341 gatttttgca gactgcgcca acggactgat gtccctgccc aacctcattt ccctgctggc
     2401 actttccggc gtgcttgtcc agcaaacgcg gtactacctc tggcagcaca ggctggacga
     2461 ttacgacgaa tcccacatac cggaaggaaa ataacgattt ttttaactcg gattcaaata
     2521 attcttgcct ttcggcttcc ggtagtattt acttcgccgc gcagtccctg caaacctaaa
     2581 agtcgcgttc gtctagcggt ccaggactcc cgcctttcac gcgggcaaca cgggttcgag
     2641 tcccgtacgc gatgccagct ttaccgccgc ggcagttacg ataattccgc ttttcccctt
     2701 ttcagggaag catattggaa aaacgtaatg gctgcggttt tcttttggcc cggttttccg
     2761 gattcaggaa agcgcggcag aggcgccgga agcggaatcc cccccctgct tttaaatgcc
     2821 ggaacatgtc ttacatcccc cggatttggc tttcagaaaa ccccgccatc ttttaaaatc
     2881 ctttacacgc aaacgccatg gattacacac ctctcattga aaaacgccgc cagcgcctgg
     2941 aagaattgga aacagtcatt gccgaaccgg acttcttcaa tgaccagaaa aaggcctcgg
     3001 aaatcatgag agagcaccgc cgcctgaagg aactcatgga aacatgggat tccctgaacg
     3061 ccacggaaca acagctggcg gacaaccagg aactggccaa gacggacgat ccggaactgg
     3121 cggaactggc cgccctggag attccagaac tggaagccgc cctggaaaaa ctgcgaagcg
     3181 acgtgcagta cagcctgctg ccccgcgaca cgacggaaga ccgggacgcc atcattgaaa
     3241 tccgcgccgg aacgggcggg gatgaagcct ccctgtttgc cggagacctg ctgcggatgt
     3301 accagcgttt tgcggaagaa cgcggctggc gcttcgagca tctggaaagc agcccgtcgg
     3361 acgtgggcgg attcaaggaa gtcgtctgcc gcattgccgg ggaagaagtg ttccgattcc
     3421 tgaaatatga aggcggcgtg caccgtgtgc agcgcgttcc cgctacggaa acgcaggggc
     3481 gcatccatac ttccaccgcc accgtggccg tcatgccgga agcggaggaa gtggacatag
     3541 aaatccgccc ggaagatctc cgcatcgaag tatgccgttc cggcggagcc ggaggccagc
     3601 atgtgaacaa aacggaatcc gccgttcaga tatggcacat acccacgggc gtttatgtcc
     3661 ggtgcgagga agaacgcagc cagatgaaaa accgagaaaa aggcatgaaa atcctgcgcg
     3721 ccaagctctt tgaagccaaa aaacgggaag aggcggaaaa atactccgcc gcgcgccgca
     3781 atctcatcgg ctccggcggc cgtgaagaaa agatacgcac atacaacttc ccgcagaacc
     3841 gtctgacaga ccaccgcatc ggctacactt cccacaatct ggacggtatc ctgatggggc
     3901 agctggaaga cctgattatg gccctccagc atgcggaaat gcaggaacgc ctggcggagg
     3961 ccggaatgtc ctaacttcct ttttcctttc atgaaaactt tactggaagt ccttcaggcc
     4021 ggcacggatt atctggcccg ccaggggtgc gacgaagcac gcgccaccat gcagcatttg
     4081 ctggcccatg tcctgcactg caaccgcacg gctctttact cccagtttga caggcccgtg
     4141 gaagaagcgg agctggcacc cctgcgcgaa ctgctgaaac gcagggcagc gggagaaccg
     4201 ctgcaacatc tgctgggggt cacggaattt ttccggaggg atttcctgac ggatgcccgc
     4261 gccctgattc cccgcccgga aaccgaggaa ctggtggaaa tggtgttaaa gaaaatcccg
     4321 gaccatccgg tacgcatcct ggacatgggc acgggctccg gcgttatcgg cgtcacgctg
     4381 gcgctggaac tcaaggagcg cgccggagaa gtcgtgctgg cggacatttc cccgcaggct
     4441 ctggatctgg ccctggaaaa tgccatgcgg ctgggagcca gagtctccac catccagacg
     4501 aatctgttcg ccaacattcc ccaggaaaaa acggatccgc atgcggagga tgccgattct
     4561 gctccggagg gagaaaaaga ggaaaacggg cggaacaagc tctttgacgt tatcgtggcc
     4621 aatctgccat acattgcaga gggggaaaaa cttgccccgg aagtcatgaa agacccccat
     4681 acagccctgt tcggaggccc taaaggctgg gaaatcattg aacgctttct ggcccgcgcc
     4741 cgggattacc tgaacgaaga cggctttgta gccctggaaa tcgggtacga ccaggcatcc
     4801 gtcgtaacgc aaatcatgga tggatacggc tacaattaca ttgaagttct gaaggacatg
     4861 agcggcgtct cccgtttccc tttcggctat cgttaatgtt atcctccttt tcactccatg
     4921 caggcggcgc gcattctggt tgacggacaa agcgatctgg tactggatta cggtattcct
     4981 ccggaagcgg gagatgtcaa gccgggctgc cgcgtacagg tgcccctgcg caacagaacg
     5041 gctaccggaa ccgtgcttac gctgtcagaa ccggccccgg cctggaagga caggctcaag
     5101 cccattctga aactgatcga tccggagccc ctgatttccc cggtgatgat gaatctggcc
     5161 tcatgggcgg cggactatta ctccgtggct ctggatcaga tgatccggtg tctgctcccg
     5221 gaaaccgttc gtcaggaaaa tacggcggaa aaaatgcgca aaatggtgta tctggaaaaa
     5281 actccggcac gggaggaact ggacgccctg taccgcaaag caccccggca ggcccaaatg
     5341 ctggattatt tctcatccgc gaaacaacag tccgcccccc tggcggcatt cggcgccgga
     5401 gccctgaacg ttgcccgcag cctggaagcc aaaggcttca tctccctgaa agaagaggcc
     5461 gtgcaccggg accccagcac cggggagcaa ttcgtcccca cccagcccat gaagctgaac
     5521 agccagcagc agaaagcgct ggaagaaatc acggccatgt gcgcggcaga gcgcaaaaaa
     5581 ccggtcctgc tgcaaggggt taccggttcc ggcaagacgg aggtttactt acaggccgtt
     5641 tcccaaatag taaaatccgg aaaatccgcc ctgatcatgg tgccggaaat ttccctgacg
     5701 ccccagacgg ttcagcgttt caaatcccgt ttcgcggaac tgccttcctc cgtggcagtg
     5761 ctgcacagcc tcctctcgga cggggagcgc tttgacgaat ggcatgccat ccgttccgga
     5821 aaagcccgca tcgtcatcgg tccccgttcc gccgtcttcg cccccctcca aaatctgggc
     5881 ctggtgattg tggatgaaga acacgacgcc tcttacaaac aggaaagctc cccccgctac
     5941 cacgggcggg atctggccgt gctgcgcgcc catctggaaa attgcgccgt gcttcttggc
     6001 tctgccactc cctccctgga aagcatccat aacgccctga ttggaaaata ttccctggta
     6061 aaactgacgg aaagggcgga cggccagcag ctcccgctca ttcgcatcct ggacatgaaa
     6121 acggaaggaa ggaacaaatc cggtcccaac gttatctccg aacgcctcag gatgtccatt
     6181 gaccggcgtc tcgacaaggg ggaacaggtc atcctgctgc tcaacaggcg cggattcgca
     6241 cgctccatcc aatgcccgga ctgcggccac gtagtcacgt gcctgcactg ctccctgccc
     6301 ctgacctacc accgtacgga agaccgcctc atgtgccacc tgtgcggatt caaagccctc
     6361 ccgccccgtt cctgcccgga atgccggtcc gccaacatcc tgctccaggg gtacggaacc
     6421 cagaaagtgg aggaactcct gcgccgcact ttccctgcgg cgcgcatcac gcgcgtggat
     6481 gcggacgtgg cccggcggaa aaacgccgtc cggaccattc tgaaccagtt tcgcgcccat
     6541 aagatagata ttctcctggg cacccaaatg atcgccaaag gtttggattt tcccaacgtg
     6601 acgctggtag gcgtactgaa cgcggacctg gggctccaca tcccggatcc ccgtgcaggg
     6661 gaacgcactt tccagctcct gacgcaggta gcgggccgcg ccggccgcgg cgatctttcc
     6721 ggagaagtca ttatccagac ttttacgccc cagtccccct ccctgcaata cgcccggcac
     6781 catgacacgg acggctttgc agcccaggaa ctggaaatgc ggcgtacttt cgacctcccc
     6841 cccttcaccc atatcgccgt cttgaccata cgttcccagc atgaaagcat ggcggaattc
     6901 gccacgcaaa ccctcgcggc ccgcctgcgc ggcatgctcc cccctcccgc cacgatgacc
     6961 gaccccatgc ctgcccccat cccccgtgcg cacggacagt tcagattcca gattacggtc
     7021 aaggggccat ccgcccgcat cctctcccgc actctccgga aactggtgca ggaagccggc
     7081 ctgggggaag acctgacggc tgtcattgat gtggatgcca tgtcattcat gtaagccgcc
     7141 ttgccaaaac cggccgggaa cattagactt ccggcacaat acaacttact attatgctgc
     7201 ttcacttcta caagatgacg ggtgccggca acgactttgt catggtggac aatcgtgacc
     7261 tgagcctttc ctccgtgctg gacaaagaaa ccatagaagc cctgtgtgac cgccgcttcg
     7321 gcattggggc tgacgggttg atcgctgtgg aaccctccca gggcaagggg ggaattgttc
     7381 gtatgcgtta ttacaatgcg gacggcggag aggcggaaat gtgcggcaat ggagcccgct
     7441 gcttcggcaa ctttgccgcc gccctgcttc atcacgacaa gacagccccc cttccttttg
     7501 aaaccatggc cggcgtcgtc accgcaacct ttgaaaaaga cggcaacgtc accgtaaacc
     7561 tgaccaatcc ccactccatg cagctcttcg tgctgaatgc cgatccgact gtgggggcgg
     7621 atgtccattt cctgaacacg ggcgttcccc atgccgttgc gtttctggac aagctggagg
     7681 acctgaacat ttccaggctg ggagcctatc tgcgttacca tgaggccttt tcacccaaag
     7741 gcaccaatgc caacttcgcc agaatcctgt ctccggggca cattgccata cgtacttacg
     7801 aacgcggcgt agaggatgaa acgcttgcct gcggaacggg aatgaccgcc tgtgcgctgc
     7861 tgcatgccgt attggcggac gccccctccc ccattcaggt ggatgtagcg ggcggagaca
     7921 ctcttaaagt cggattccaa cgcacagggg accgttttac tgacgtcacg ctgaccggac
     7981 cggcagacct tgtgttcacc ggagacattc agatctaaac agcttcgacc tgaaaaggcg
     8041 gactccttcc catcagggaa attatctgtt cagcagggaa cattccgccc gcacataacc
     8101 gccgtaatta ttgcagaacg ccttgtacac ggcagggccc atattcgttt cgcaatagta
     8161 ctttacggta atacagccgt tctcacgttc catattaaga aaacggctgg acagcagtac
     8221 cagattgctc tggtaattcc acaaagccat gtcacgctcc atttcctcat gaatcctgtc
     8281 gcatataaag ctctccggag gaggaaacag aatggcgcag acaacaccga tcagacagat
     8341 tcccggaatg acccctacgg caagaacccc gtgacatttc ctgaccaggg gaaggtgatt
     8401 cctgaatttc cggaaaatgg tgaaacggaa taaataaccc gccagcagcg ccaccgggaa
     8461 gaacacattc acgtactcaa aagccaggct cattcctcca agaacgaaaa aaccgatgcc
     8521 cttccacacc ttccggtaaa caggaatgca ggcgcaaaga aacacgtaca gaagatatcc
     8581 cagcaggcaa aggaaaggca gaagcgttcc cgtaaaaata tgctccacca tgcagtccca
     8641 cggatattcc ctgctgggag cgaaggcttc atggaaaaac atgcatacca tgctcaaaag
     8701 caccagcgcg gcaaggagga tggtctttac tttttttgac tcatacaaaa gccacagcaa
     8761 aataactccg aacggcaata aatagcaacc tatactgttg aatacagtgg acaacatggg
     8821 aatatgagga aagaaattca tggagcggat atggataaat gagcggttaa caactaaaat
     8881 gcagcagcgg cttcagcaac agtaattgga aataaaataa attcaaacac tcgttttcaa
     8941 ataacccata aaaaatcata ttcttttcac gaaaagccgt tcagccgggc tctcccactt
     9001 gacaaaacaa ccctctgaaa aggatagtaa cgctcttgtg aaacactccc ttttcatcca
     9061 ggccgtcctc cttccggttc tcttctgcct tccccaatgc agccgcaaac cgctccttcc
     9121 ggaatatacg cctctgtgtt ccggaatggt gccgggggaa cacactgccc accgggccct
     9181 ccgggcacgg gaaaacagaa cctaccgggt cggtccgaaa ggcggcgtat attacatcaa
     9241 ttcccataat aaaaaagttt acatcaaatc caggagggtg caatagaaga ccacacggtt
     9301 tctccgtaat tttccaaatg ggaaacaaat aaccgtccta tcatgttttt attgtataat
     9361 tgagaaaata ttccgttcaa aaaactcatt aaactcattt tcttcttgcc aaaatggaag
     9421 aggacgggta atgtctgccg caccacaaca ctaaatggtg tcggtagttc aatggtagag
     9481 ccccagattg tgattctggt tgttgcgggt tcgagccccg tccgacaccc catccccttt
     9541 ccttcataaa aagagcactt atagtgccca gccctttccg catgcgtaat ccagaattcg
     9601 tcaaagccgc tttttccgct attgccccgc gctatgtggc taccaaccat gtgctgagca
     9661 tgggtgtgga cctgctatgg cgccagcgcg tcgttcagct tgtctccgaa tggaaaccgg
     9721 aaaaccttct ggatctggcg actggaacgg gagacctggc cctggccatc ctgaacgcga
     9781 tgccggaaat ccgcctcacc gggtccgact tctgccagcc catgctggac atagcggcca
     9841 ggcggggact ggatcatctg gtttgcgcag acgccatgaa cctgcccttc ccctccgcct
     9901 cctatgatgc ggtcactgtc gcctttggcc tgaggaacat ggccagctat ccggacgccc
     9961 tgcgggaaat gggccgtatg ctgcgtccgg ggggccatct gctcattctg gatttttccc
    10021 tgcctgaaag cctgttgaaa agcccttacc ggttttatct gcaccgcatc cttcccgtca
    10081 tcgcagggtg gatgaccggc caccgggaag catacgacta cctggcggac agcattgaag
    10141 ccttcccctg cgggcaggcc atgaaggctc tgctgcggga atgcggctac agcaatatca
    10201 cggcggaagc tctgaacggg ggaattgcca gcatttacac agcccagcga tgagtgcggg
    10261 ggacgcaaga gaaggcatca tacgcggcaa ggcggcacgc gcccgcctgg gcggaaaact
    10321 tgccggacta tccctgcccc ggcaaatcgc cgtgattgct ttctggccat tcctggaaca
    10381 gctgctgagc tttttcgtca cctcgtccga ccttttcatc gccactaaaa taggggtgaa
    10441 cgcccaggac accatcaaca tctctgacgg catgggcgcc gtcgtcttca tcatgtggtt
    10501 cggcttcgtc atccaggggt ctatcatgat gggagccaca gccatcgttt cccgcatgac
    10561 gggcgccagg gactatcccc aggcccagca tggcctgcac caggccgcca tgctggggct
    10621 cctggcagga accatctcct gcggacttct cttcacatgc agcgatttca tggtcacgca
    10681 cgtactgacc atgaatgaaa ccgcccgggc ctacgccctg caatacgttt atatggccgc
    10741 atttgcggcc ccgttcagcg gcgtggtctt tgccatcaat gccgccctgc gaggctccgg
    10801 agacacgcgg cttcccttct ggatcatgat gagcgtaggc atcctcaatg tcatcttcag
    10861 cgtcatcttc gtctttgcgg acgctcctct gggcggctgg cgcatcggag gaattgcggc
    10921 aggaacggtc tgcggctacg gcatcagcat gtgcatcctg ctgttcatca tgctgcggcg
    10981 cagaaaaaaa atcttcacgg gcaggaaaaa tgaatccctg gaagaacttg tccgggaacg
    11041 gggagaacac tatgccccgc ccctgtacct cagcttttcc catctgcggc cggacatggg
    11101 catgcagaag cgaatcctga aaatcgggct gccgcaggcc gtggaagtct tcggcatgtg
    11161 gggcatccag atgttctgcc tttccatcat cagtgaactg cctgttaaag gcgtgctggg
    11221 cgtccacaat attgccgtcc gcattgaatc gctcagcttc ctgcccggct tcgccatcgg
    11281 catggccgct tccacgctcg tggggcaata cctgggcgcc cggaacgcgc ttatggcccg
    11341 catcaccatc tggaaatgca tgcggtacgc cgtcatcttc atgacaggcc tgggagtgct
    11401 cttcagtgcc tttcccgccc tgttcatgga gatattttcc aacggcagca cgaccctcat
    11461 tgataccgga atccccgtgt tgcgcaccat gttggtggtg gaacccttct tcgccgcctg
    11521 catcgtgatg aaaatgtccc tgcgcggcgc gggagacacc cggagagtca tgcttatttc
    11581 ctacggcatt atgggctttt tccgggtcgt ctgcacctgg gtatggttca ggctggcacc
    11641 ggaaaccatg actttgtggg gcatctggct ggttttcgcc tttgaaatgg ccgtccaatc
    11701 cgccatcctc tataaaatcg tcaaggggcg gagctggacg aagctgcaag tctgaacctt
    11761 tctccggcct ctc
//
LOCUS       DAOMSU010000043        11652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_296_length_11652_cov_2.924463, whole genome shotgun sequence.
ACCESSION   DAOMSU010000043 DAOMSU010000000
VERSION     DAOMSU010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11652
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_296_length_11652_cov_2.924463"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            573..947
                     /locus_tag="PK077_03260"
     CDS             573..947
                     /locus_tag="PK077_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23347.1"
                     /translation="MKKWLPFCEDLRKQYKHTPTPVEYSILLVLQPPPIEGDVPAFTQ
                     ALGDFPDFYMKTNSLISYYDQSFVRRRLSLHKKAETAIVLLDGNGNIIWKDDGGFTAS
                     RAEHLKVILETLSPLAPRTRTH"
     gene            1023..2135
                     /gene="aroB"
                     /locus_tag="PK077_03265"
     CDS             1023..2135
                     /gene="aroB"
                     /locus_tag="PK077_03265"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HRN23348.1"
                     /translation="MPTISLALGERSYDIHVENGALDRTGEFAYRAGLDGKIAVITDS
                     CVAPLYAERVMKSLESAGFMPSLHVVNAGEASKNMLQAQELCSELVRAGLDRTGCIAA
                     LGGGVVGDLAGFIASIYYRGIPFMQIPTTVVAQVDSSVGGKTAVNIPEGKNLVGAFHQ
                     PSVVVIDPTTLVTLDRRTLAEGLAEAVKHAAIKDASMLHELKGLGPELSIGFSLNTIA
                     RLPDLISRNVAIKARIVENDELETLDIRALLNFGHTIGHGIEAAVPYGTILHGEAVAL
                     GMRAALFLSERKAGLSSSDANKILRTLQALELPLVLPDGIDTATVLQKTASDKKFRAG
                     IIRFVLLSKAGEAGISTKITREDMAEAIEELRRPLD"
     gene            complement(2352..2768)
                     /locus_tag="PK077_03270"
     CDS             complement(2352..2768)
                     /locus_tag="PK077_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23349.1"
                     /translation="MMKRNLIPFTAFLAALVFVACDGGKKQDHPRDVSVPSEQAVPKT
                     PGEKILAVLLLIQDQATANAHAAEVKELAASLPGDIPNEEAVNIANELMRLASRECYG
                     SSSLEDALKGIHFDTGEYTGSVPLDVDGFEPTAEEP"
     assembly_gap    2935..3021
                     /estimated_length=87
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3100..4377
                     /locus_tag="PK077_03275"
     CDS             3100..4377
                     /locus_tag="PK077_03275"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRN23350.1"
                     /translation="MGYPIWNRRRFLKTAAGTAGFLAAMPCLNAAEVFTDTKPLKVGL
                     VGCGGRGLGAMKNALDADPGTIAWALADVFQERIDFGAKLLAEQYGNRAQLDRSRLFS
                     GLDSYKRLLQTDIDVVLLCTPPAFRPEHLAAAIDASKHIYAEKPVAVDVPGVLSVLES
                     ARLAKLKNLVILDGFCWRYDKANEEAHRKLSSGLLGRVLSFDGLYYTTPPKSPLALDS
                     RPSSDTDVAWALRNWTAWNWLSGGQFVEQIVHTIDGMVWSMNEQLPLAAFGSGGRAQR
                     RDDGDVWDHYDVYFEYDHNVTAHISCRQWVGCHGEIVDRTICEKGMLVTPYRPYIQAN
                     NRWRFRGERGNMYADTHVAFYRFLRSGTWTQTLESAANKTLAAIMGREAAHTGQRITW
                     EQIKKSTARLMPEDLTMDTPLPPARIPRPGRTA"
     gene            4409..5113
                     /locus_tag="PK077_03280"
     CDS             4409..5113
                     /locus_tag="PK077_03280"
                     /inference="COORDINATES: protein motif:HMM:NF025875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HRN23351.1"
                     /translation="MANIFPPNTNKRFYGIAALVALAAAFVLGGIYYVNFSIPEYEPM
                     QPVRFSHKLHAGDLKMSCTACHSAAQRSPRAGIPDTKSCLGCHQHILPDSPLIAPLRE
                     AADPQYPGYTGEPVRWVMVNRLSGHAYFNHMAHLNRGIGCTSCHGDVAGMERIRAPRD
                     ARMQWCLDCHRNPAPHLRPLEETASSHYSAADYLRTHSIRDEEGKSIQTPLQLGNFLK
                     RQWTIQPKTDCTACHH"
     gene            5488..8361
                     /locus_tag="PK077_03285"
     CDS             5488..8361
                     /locus_tag="PK077_03285"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HRN23352.1"
                     /translation="MKWMGAGAALAGIGLPACRRVEKYLVPYNEGPEWSVPGVETAYA
                     TCLTMGGNAQPVLAACYEGRPVKLIPSLQYPEGPGLPAAAQASILDLYDPGRSKHILF
                     NGKPASEDEFRGAFSSWSRNLRNGGGIGLLLPPSTSPLLHSMLEEITRKNSLVRIYRY
                     SPVPEPGSGMQAGLPEEVRFRVRFARARRILSLDCDFLHQNPYGNTRDFIASRSPEGL
                     YYKEENRNRTRLYAVEGRVSLTGAHADHRLPVPPARLAFFLEELFRYLSSKKNSGQTL
                     PPPRQTDHPLTEKELNWLRHCADDLFSHPGESVVLLGDNHPELSGIVWKLNCLLGSMG
                     TCIQLLKAPRPTPYGALDDLVRDIRGKKVEIVFLLDAGNPVLDSGRSSGLSEALNKVE
                     SIHLGMYEDETSRVCRWHLPAAHFLESWGVERDYQGRFCYRQPVILPLYGGISPEEVL
                     SGLLSSKGHLSTADNSPTHLSPVYHRARKCFERAVNPENKTAAWAQALQRGYSEETAY
                     APLFPQEETALGTAMMQTPAAPFGGHGKKLGTGMLELQFRADYSIGDGRWKRNAWMQE
                     CPDPITGVSWAASAQVSPATFLRLGGSDSGPMHCTLTAPSTQMEVILCPIPGVADNLI
                     ILPLGYGGINHVAERQENSGGYELRRQVEKTEAYGIAPEQIALSALRERTEAIQSPVV
                     QPAPFSLHPGPSRLPPPPPGADAVYQWKMAIDTSRCIGCNACMIACRAENNIPVVGRD
                     QMARGRALDWIRIDRYFTEEGTLTSIPVACQQCGRAPCESVCPVNATVHTAEGLNAMV
                     YARCWGTRYCATNCPYKARRFNFFDYAKASEQATRLQRNPNVTVRSRGVMEKCTYCVQ
                     MVERAKIRHKSRLMKEHPGQPSTSIHVTAQDMLLPDGAAQTACQLACPMGAITFGNVL
                     DPAAAVFRAKSLPRHQDLLSCLGTSPGTGYLVPARNPNPAMEK"
     gene            complement(8469..8912)
                     /locus_tag="PK077_03290"
     CDS             complement(8469..8912)
                     /locus_tag="PK077_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23353.1"
                     /translation="MIQTYQVLPVVAIVCSASAFAQNAADPVQMVKQNIDLISSANKV
                     LDDVKDNAAVEIAIKQLNALTQQAKQLDKSMEKMKLTSEQAIRITKLNGDAQDTIVDM
                     LENCERIQKDKLMTPALLKAVNDFADAANIDVVETITTVEQIVED"
     gene            complement(9050..11149)
                     /gene="recG"
                     /locus_tag="PK077_03295"
     CDS             complement(9050..11149)
                     /gene="recG"
                     /locus_tag="PK077_03295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRN23354.1"
                     /translation="MVHDKGYVLGFMAGELLLTSSLEECVFIRPRELAAMRAAGMASV
                     RDLLFMLPRRYEDRRMFDRYDSLSSGVPVCLRGRVVDVGWKGWGGRGGKGRGRYVEAV
                     LVDEQSLGQARFSCLWFSMPGVARMLCAGQEMIVYGRMKPYGKKLSIVHPDFEIIREG
                     DEQSIHLNRIVPVYGGRMGIAVRRLREIVWETLSRLSPAPEPEVYEFVPDTPCKTALK
                     DLHFPETAEARDRARRRFALEECLAQQLNVAYRRRRADEVPGMRTAGSSHLVKDLADS
                     LPFELTEAQKRCVREIYRDMKAPRSMNRLLQGDVGSGKTLVALCAMLLAVEHGYSAVM
                     MAPTQILAEQHYLKFRQMLDKLDVPVSLVTADRKEESHVSFGKQGGIVVGTHALLYGK
                     NVPERVGLVVIDEQHKFGVNQREKLIDRGERPDVLVMTATPIPRTLTLTFYGELDVSI
                     LDGVPGGRGAVVTAIRTEKDKEKVLAFVRNQLEEGRQIYVVSPLIDGEDSRKGKAVTK
                     EWDEWKALLPHVDVGLLHGRMSSEEKEAVMKDFRSNRISVLVSTTVVEVGVDVPNATV
                     MIINNAESFGLSQLHQLRGRIGRGSHKSYCILMTDARPEDEQWEKLRIVETTTNGFDL
                     AEQDLRLRGPGDVLGTSQSGLKGVRFEEWLLDARLIHRGRQLAEAILAEDPNLESAKY
                     RPLRFLLEDGAGRRVAG"
     gene            complement(11163..>11652)
                     /locus_tag="PK077_03300"
     CDS             complement(11163..>11652)
                     /locus_tag="PK077_03300"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRN23355.1"
                     /translation="RSRARRAAHSMHPRFITIHSTANPKGDAAAHARALKRGAMGSLN
                     WHFTVDQYCAVQHIPLNETGRHADRGGPGDMYSIGIEMGEVRSHNPIVTWNRSAKLTA
                     VLMKQYHIPLRNVVPHYYWTGKNCPAPLLTNGRPGHKWSWFISRVDYYRRCLETRPAA
                     AL"
BASE COUNT         2549 a         3586 c         2905 g         2525 t
ORIGIN      
        1 gccgagaaat ctaccatacc acaccttttt gtcaattttt ttcgagatgt tttttgcgtc
       61 cgcttattcc caccacccct ccaatactca cataacctac atatcatgaa cagtatatgc
      121 ttttgtcata ctgcctcttt ccaccgcatc tttttttgaa cgtccctctc ttccctactc
      181 ttgtacccca cctttcttcg agaaaaactt ataacaggtc tatatattcc cttttccatg
      241 aataccgatt ttctaatcgg ccggaatttt cagagaaaga gagccttcca acagaaaaaa
      301 ggcccttccc cgcaccaatg aacataccct gttcggcaac catcgacatg aacattattt
      361 cttcgaattt tgtctaacag ggcatgaaga atcaggaaat acacttgcca acgcccaatc
      421 ccgagcatcc ttatggagga ggaaacggac gttaccgttt tccggatatc aacggcatga
      481 atttactggg agacaatatt tccctgcctc ccatcgttcc aaaaagtttc catatcatgc
      541 tgactgtttt tcacccggaa actttgtacg gaatgaaaaa gtggcttccc ttctgcgagg
      601 acctccgcaa acagtacaaa catacgccaa ccccggtgga atatagcatt ttgctggttc
      661 tgcagccacc cccaattgaa ggtgatgtcc ccgcctttac acaggctctg ggggattttc
      721 cggattttta catgaagacc aattccctga tttcctatta tgaccagtct tttgtacgcc
      781 gcagactttc ccttcacaag aaggcggaaa cagcgattgt ccttctggac ggcaacggca
      841 atatcatctg gaaggatgac ggcgggttca cggcctcacg cgcggagcat ttgaaagtta
      901 tcctggaaac tctttctccg ctggcaccca ggacaagaac gcattaaaag gagaatgcgt
      961 cttcttcgcc tcatccacac tttccgtttg aggttccctc cggaatatcc catactggcc
     1021 tcatgcctac catttctctt gctctcggtg aacgttccta cgacatccac gtggaaaacg
     1081 gagcactgga ccgtaccgga gagtttgcct accgcgccgg tctggacggc aagattgcgg
     1141 ttatcacgga cagctgcgtc gccccacttt atgcagagcg cgtaatgaag tccctggaga
     1201 gcgcgggatt catgcccagc ctgcacgtag tgaatgccgg ggaagcttcc aaaaatatgc
     1261 ttcaggccca ggagctgtgt tccgaactgg tccgggccgg gcttgaccgc accggatgca
     1321 tcgccgctct gggcggcggc gtagtagggg atctggccgg cttcatcgct tccatttatt
     1381 accggggcat ccccttcatg caaattccaa caacggtggt cgcccaggtg gacagttccg
     1441 taggaggaaa gactgccgtc aatattccag aagggaaaaa tcttgtagga gcctttcatc
     1501 agccgtccgt cgtggtaatc gaccctacca cgctggtgac tttggatcga cgtacgctgg
     1561 cggaaggttt ggcggaagcc gtcaagcacg cagctatcaa agacgcttcc atgcttcatg
     1621 aattgaaagg gctggggccg gaactctcca tcggcttctc cctgaatact attgccaggc
     1681 ttcctgacct aatctcccgg aacgtggcca ttaaggcccg cattgtagaa aatgatgaat
     1741 tggaaactct ggatatccgg gctctgctca atttcggcca caccatcggc cacggcattg
     1801 aagctgccgt cccttacgga accatccttc acggggaagc agtggcgctc ggcatgcgcg
     1861 ccgccctgtt tctgagtgaa cggaaagccg gtttaagttc ttccgatgcc aacaagatcc
     1921 ttcgcacgct tcaggcgctg gaacttccct tggttcttcc ggacggcatt gatacggcaa
     1981 ctgttcttca aaaaacggca tcagacaaaa agttccgggc cggaatcatc cgctttgttc
     2041 tgctttccaa agcaggggag gcaggcattt ctacgaaaat tactcgggaa gatatggcgg
     2101 aagccattga agaattgcgc cgccccctgg attgagttta ccacccgtgg ccctttgttc
     2161 tctagcaaga aaactccggg agtcgggctg tacagatatt ttccccttct cttcgcgcat
     2221 tttcccatca ggaagaggaa aggatttacc gggggacctg ataccatatc aaaccgccct
     2281 tcattccggc aaaacggaat gaagaggaac attgtagtaa ttacccaatt cccggcacgg
     2341 ggaatttatt tttacggctc ttccgcggta ggttcaaatc catccacatc cagaggcaca
     2401 ctacccgtgt attccccggt atcgaaatga attcctttca aagcatcttc cagagacgaa
     2461 gaaccataac actcccggct ggccagacgc atcaattcat ttgcaatatt tacggcttct
     2521 tcattgggaa tatccccggg cagggaggca gccagctctt tcacctccgc cgcgtgggcg
     2581 tttgccgtag cctgatcctg aataagcagc agaacggcca gaattttttc tcccggagtt
     2641 ttaggaacgg cctgttcgct gggaaccgaa acatcccggg gatgatcctg cttcttgccg
     2701 ccgtcacagg caacaaacac taacgccgcc aaaaacgccg taaaaggaat gagatttcgc
     2761 ttcatcatga tggttggttg aaaagccgga ggcctcattg aattcatcat gaacactgag
     2821 gattccggag tcaaatcagt ttttatcctt aaaaactgtt tttcaggcaa catatctctt
     2881 taacctctgc ctctcgttct gaacgcagga aacagccctt cccatttaat aggcnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn ncggttgcgc ctattttttg tgtcaccatt tcaatacgac
     3061 gtttgatttc acaggacgtc accgtataat tcccctgaga tgggttaccc tatttggaac
     3121 agacgccgtt ttttgaaaac cgccgcggga acggccgggt ttctggcagc catgccctgt
     3181 ctgaacgccg ctgaggtttt tacggacaca aaaccgctca aggtgggcct ggtaggctgc
     3241 ggaggccgag gcctgggtgc catgaaaaac gctctggacg ccgatcccgg aacaattgcc
     3301 tgggcattgg cagacgtgtt tcaggaacgt atcgatttcg gcgccaaatt actggcggaa
     3361 cagtacggca accgagccca gctggacaga agccgtctgt tcagcggcct ggacagttac
     3421 aagagattgc tgcaaacgga tattgacgtg gtgctgctct gcactccccc cgctttccgt
     3481 ccggaacatc tggcggcggc cattgacgcc agcaaacata tttatgcgga aaaacccgta
     3541 gcggtggacg ttcccggcgt gctttccgtg ctggaatccg cacggctggc aaaactcaaa
     3601 aatctggtga ttctggacgg cttctgctgg cgctacgaca aggcgaatga ggaagcccac
     3661 cgcaagcttt cttccgggct gctgggccgt gtcctctcct ttgacgggtt atattacacc
     3721 actcctccca agtctccgct ggccctggac tcccgacctt cctccgatac ggacgtggca
     3781 tgggcgctac gcaactggac cgcatggaac tggctgagcg gaggccagtt tgtggaacaa
     3841 atcgtccata cgattgacgg catggtctgg tccatgaacg agcagcttcc tctggcggct
     3901 ttcggctccg gaggacgcgc tcagcgccgg gatgacggcg acgtatggga tcattacgac
     3961 gtttattttg agtacgacca taacgtaacg gcccacattt catgcagaca gtgggttggc
     4021 tgccacgggg aaatcgtgga ccgcaccatt tgcgaaaaag gaatgctggt tactccctac
     4081 cgcccctaca tccaggcgaa taaccgctgg cgtttccgcg gggaacgcgg caatatgtac
     4141 gcagacacgc atgtggcctt ttaccgtttc ctgcgctccg gcacctggac ccagacactg
     4201 gaaagcgcgg ccaataaaac cctggctgcc atcatggggc gtgaagcggc ccacacaggc
     4261 cagcgcatta cctgggaaca gattaagaag agtaccgcac ggctgatgcc tgaagacctg
     4321 acgatggata cgcccctgcc gcccgcccgc atcccgcgcc cgggcagaac ggcctgacgg
     4381 aggttccagc cctttcactc tacggttcat ggccaacatc tttccaccca ataccaacaa
     4441 gcgcttttac gggattgccg ccctggttgc gctcgctgcc gcattcgtac tgggaggaat
     4501 atattatgtt aatttcagta ttccggagta tgaacccatg cagcccgtcc gcttttccca
     4561 caaacttcac gccggagatc tgaagatgag ctgcacggct tgccatagcg ccgcacagcg
     4621 ctcacccaga gccggcattc cggatacgaa gtcctgtctg ggctgccacc agcatattct
     4681 tccggacagt ccgctcatcg cccccctgag ggaggcggcg gacccgcaat accccggtta
     4741 cacaggagaa ccggtccgct gggtgatggt caaccgcctg tccggtcacg cctattttaa
     4801 ccacatggcc cacctgaacc ggggcatcgg ctgcacgtcc tgccatggag acgtggcggg
     4861 catggaacgc atcagggctc cacgcgacgc ccgcatgcaa tggtgtctgg actgccaccg
     4921 caatccggct ccccacctgc gcccgctgga agaaacggcc agttcccatt attccgccgc
     4981 agattacctc cgcacgcatt ccatccggga tgaagaagga aaaagcatcc aaaccccgct
     5041 tcaactggga aatttcctga aacgccaatg gaccattcag ccgaagacgg attgcaccgc
     5101 atgccatcac tgacctccat ggcctgtttg acatccgcgg ctccaaagtt attcctcttt
     5161 gcgttcccgg atgctgcgtc ataaaaagac caaagggctt ttcattaaat gattccttcc
     5221 ttgaatccgt aatcaatgac attgaattcc acaagaaaga tttcctcctg ttccctgttt
     5281 atccgttatt ggcccaaaga taaagactac cgtcaatcac cacttgattt catccttcgg
     5341 atttcctaaa aaaagccatc cctgtttcat caatccccat cattacccgg tttgactccc
     5401 ctccctccat tttcccagaa tgaagttccc cagaaccacc ccgcttttct tccggaagaa
     5461 gaacggggga tgacgcggcg ttccttcatg aaatggatgg gtgcaggggc agccctggcg
     5521 gggatagggc tgccggcctg ccgccgtgtg gaaaaatacc tggttcccta taacgaaggg
     5581 cctgaatggt ctgttcccgg cgtggaaacg gcttacgcca cctgcctgac catgggagga
     5641 aacgcgcaac ccgtactggc cgcctgttat gaagggcgcc ccgtcaaatt gattccctcc
     5701 ctgcaatacc cggaaggccc ggggctgccg gctgcggctc aagcctccat tctggacctt
     5761 tacgaccccg gccgcagcaa gcatatcctg ttcaacggca agccggcctc cgaagacgaa
     5821 ttccggggag ccttttcttc ttggtcccgc aatcttcgga atggtggcgg catcggcctt
     5881 cttcttcccc cctcgacttc tcctctgctc cattccatgc tggaggaaat caccaggaaa
     5941 aattcactcg tgcgcatcta ccggtattcc cccgtgcctg aaccgggttc cggcatgcag
     6001 gcggggctgc cggaagaagt acggttccgc gtgcgttttg cgcgcgccag gcgcatcctc
     6061 tccctggatt gcgattttct tcatcaaaac ccctatggaa acacgcgcga cttcattgcg
     6121 tcccgctccc cggaagggct ttactacaaa gaagaaaaca ggaaccgaac acgcctttac
     6181 gccgtggagg gccgcgtttc cctgaccggg gcccatgccg accacaggct gcccgttcct
     6241 cccgcacgcc tggcgttttt cctggaggaa ctgttccgct atctttccag caaaaaaaac
     6301 tccggccaaa cgcttccccc tccccggcaa acggaccatc ccctgacaga aaaggaactc
     6361 aactggcttc gccactgtgc ggatgactta ttcagccacc ccggagaaag cgttgtcctc
     6421 ctgggggaca atcatccgga attatccggc attgtctgga agctcaactg tcttctcggc
     6481 tccatgggaa catgcatcca gttgttaaag gctccgcgcc caacccctta tggcgcgctg
     6541 gacgatcttg ttcgggacat ccgggggaaa aaggtggaaa tcgttttttt actggatgcg
     6601 ggaaatcccg tgctggattc cgggcgcagt tccggactgt cggaagccct gaacaaagtg
     6661 gaaagcatcc accttggaat gtatgaagat gaaacctccc gcgtctgccg ctggcacctg
     6721 ccggcagccc actttctgga atcatgggga gtggagcggg attaccaggg cagattttgc
     6781 tatcgccagc ccgtcattct tcccctgtac gggggcattt ctccggaaga agttctttcc
     6841 ggcctgcttt cctccaaagg ccatctctcc acagcggaca attcccccac ccacctctct
     6901 cccgtttacc acagagcacg caaatgcttt gaacgcgccg tgaatccgga aaataaaact
     6961 gcggcctggg ctcaggcgct tcagcgcggt tattcggaag aaacggccta tgctcccctg
     7021 ttcccgcagg aagaaacggc tcttggaaca gccatgatgc aaacgcccgc agcgcctttt
     7081 ggcggccacg ggaaaaaatt gggaaccggg atgctggaat tgcaattccg cgcggactat
     7141 tccattgggg acggacgctg gaaacgcaat gcatggatgc aggaatgccc ggacccaata
     7201 acaggcgtca gctgggcggc gtccgcacag gtttcccccg ccacctttct gcggctggga
     7261 ggttcggatt ccgggcccat gcactgcacg cttaccgccc cctccacgca aatggaagtc
     7321 atcctgtgcc ccattccggg agtggcggat aatctcatta ttcttcctct gggttatggc
     7381 ggcatcaacc acgtagcgga aaggcaggaa aattccggcg gatatgaact ccgcagacag
     7441 gtggaaaaaa cggaagcata cggcattgcc ccggaacaga ttgccttgtc tgccctccgg
     7501 gagcggacgg aagccattca gagccccgtt gtgcagcctg ccccgttctc cctgcatccc
     7561 ggtccgtccc gcctcccgcc tccccctccc ggcgcggatg ccgtttacca gtggaaaatg
     7621 gccattgaca cgtcccgttg catcggctgc aacgcctgca tgattgcatg ccgcgcggaa
     7681 aataacattc ccgtggtggg gcgtgaccag atggccaggg gccgcgccct ggactggata
     7741 cgtatcgaca gatacttcac ggaggaaggg acgctcacct ccatccccgt ggcctgccag
     7801 cagtgcggca gggccccatg tgaatccgta tgccccgtca atgccaccgt gcatactgcg
     7861 gaaggcctga acgccatggt gtacgcccgg tgctggggta cccggtactg cgccaccaac
     7921 tgcccgtaca aggctcggcg cttcaatttt tttgattacg ccaaagcatc ggaacaggcc
     7981 acgcgccttc agcgcaatcc caacgtaacc gttcgctccc gcggcgtcat ggaaaaatgc
     8041 acctactgcg tccaaatggt ggaacgtgcc aaaatccggc acaaatcccg cttgatgaag
     8101 gaacatccgg gccagccatc cacctccatc catgtgacgg cccaggacat gctgcttccg
     8161 gacggggcgg ctcaaacggc ctgccagctc gcatgcccga tgggagccat caccttcggc
     8221 aatgtgctgg accccgccgc agccgttttc cgcgccaagt ccctgccgcg ccaccaggat
     8281 ctcctctcct gcctgggcac gtctcccggc acgggctacc tggttccggc aagaaacccc
     8341 aatccggcca tggagaaata aaatgccctg cccggaaatt gataaaaaac ggcccttcct
     8401 taaaaaataa ggccgccttc ttcaggaggc ggccttacca gtcaaaggtc ttttgataag
     8461 ctggtcactc aatcttccac aatttgctcc acggtcgtaa tcgtttccac cacgtcgatg
     8521 ttggcggcgt cggcaaaatc attcaccgcc ttgagcaaag ccggagtcat cagcttgtcc
     8581 ttttgaatgc gctcgcaatt ttccagcata tccacgatgg tgtcctgggc gtctccgttc
     8641 aatttggtga tgcggatagc ctgctcggaa gtaagcttca tcttttccat ggacttgtcc
     8701 agctgcttgg cctgctgggt caacgcattg agctgcttaa tggcgatttc aaccgcagcg
     8761 ttgtccttca cgtcgtccaa aaccttgttg gcggaagaaa tcaggtcgat attctgcttg
     8821 accatctgca cgggatcggc agcgttctgg gcaaaggcgg aagcggagca tacgatggcc
     8881 accacaggga gtacttgata cgtttgaatc attgttttat cggtgttcgg gttgaagaag
     8941 aaacatcttc tttgcttctt tagattcatt ccggcggata aatcgttgcc ctcttatctg
     9001 tcatgcgcct cttacaagct gaccgtcagc aaacggcaga aaaacaatct tacccggcca
     9061 cacgcctccc cgccccgtct tccagcaaaa aacggagagg gcggtatttg gcggattcca
     9121 gattgggatc ttccgccaga atagcctccg ccagctgccg cccacggtga atcaggcgcg
     9181 catccagcag ccattcctca aaacgtacgc ccttcaggcc gctctgggac gtacccagca
     9241 cgtctccagg gccgcgcagt ctcaaatcct gttccgccag gtcaaatcca ttcgtcgtag
     9301 tttccacgat gcgcagcttc tcccattgct catcctccgg acgggcgtcc gtcatcaaaa
     9361 tgcagtagga cttgtgcgac ccgcggccga tgcgcccccg gagctgatga agctgggaaa
     9421 gcccgaagct ttccgcatta ttgatgatca tcaccgtggc attcggaaca tccaccccca
     9481 cctccaccac tgtggtggac accagcacgc tgatgcggtt ggagcggaag tccttcatta
     9541 ccgcctcctt ttcctctgaa gacatcctgc cgtgcagcag ccccacatcc acatgaggca
     9601 gcagagcctt ccactcgtcc cattccttcg tcacggcttt ccccttccgg gaatcctctc
     9661 cgtcaatcag gggggaaacc acgtaaatct gcctcccttc ctccagctgg ttccgaacga
     9721 atgccagcac tttttcctta tccttttccg tgcggatggc cgtcacgacg gccccgcgcc
     9781 ctccgggcac gccatccaga atggaaacgt caagctcccc gtaaaaagtc agcgtcagcg
     9841 tgcggggtat gggggtagcg gtcatcacca gcacatcggg gcgctctccc cggtcaatca
     9901 gcttctcccg ctgattcacg ccgaatttgt gctgttcgtc aatcacgacc agccctacac
     9961 gctccggcac atttttcccg tacagcaagg catgcgtgcc gaccacaatc cctccctgct
    10021 tcccgaacga cacgtgggat tcctccttcc tgtccgccgt caccagggaa acaggcacat
    10081 ccagcttgtc cagcatctgc cggaacttca gataatgctg ttccgccaga atctgcgtgg
    10141 gcgccatcat cacggcggaa taaccgtgtt ccacagccag cagcatggcg cacagagcca
    10201 ccagcgtctt tccggacccg acatccccct gaagcaggcg gttcatggaa cggggggctt
    10261 tcatatcccg gtaaatctcc ctcacgcaac gcttctgagc ctccgtcagc tcaaacggca
    10321 gggaatccgc cagatccttc accaaatggc tggaacctgc ggtccgcatg ccgggcactt
    10381 catccgcccg ccttctcctg taggccacgt tcaactgctg agccaggcat tcctccaacg
    10441 caaaacggcg ccgcgcccgg tcccgggcct ctgcggtctc aggaaaatga agatccttca
    10501 atgccgtttt gcacggagtg tccggcacaa actcatacac ctccggttcc ggagccggag
    10561 aaagcctgga cagcgtctcc catacgattt cccgcagcct gcggaccgca atccccatgc
    10621 ggccgccata cacggggaca atgcggttca aatgaatgga ctgctcatca ccttcccgga
    10681 taatttcaaa atcaggatgc actatgctga gctttttccc atacggtttc atgcgcccgt
    10741 acacaatcat ctcctgcccg gcgcacagca tcctggctac gcccggcatg ctgaaccaca
    10801 ggcaggaaaa acgggcctgc cccaaagact gctcatccac cagcacggct tccacatacc
    10861 ggccacggcc cttgccgccc cgtccgcccc agcctttcca gcccacatcc accacccgcc
    10921 ctctcaggca gacgggcaca ccggaagaca gggaatcata gcggtcaaac atgcggcggt
    10981 cttcatacct gcgcggaagc atgaacaaca aatcccgtac ggaggccatt cccgccgccc
    11041 gcatggccgc caactctctg ggacggataa aaacgcattc ctccagagaa gacgttaaaa
    11101 gcaattctcc agccatgaac ccaagaacgt atcccttatc atgcaccatg cgcgcgtacc
    11161 cgtcagagcg ctgcggcggg acgcgtctcc agacagcgcc tgtaataatc cacgcgggaa
    11221 atgaaccagc tccatttatg ccccgggcgg ccgttggtca gcagcggggc cggacaattc
    11281 ttgcccgtcc agtaataatg gggaaccaca ttacggagcg gaatatggta ttgcttcatc
    11341 agcactgccg tcagcttggc ggagcggttc caggtaacaa tggggttatg gctccttacc
    11401 tcccccatct caatgccgat ggagtacatg tctccgggac cgccccggtc tgcgtggcgg
    11461 cccgtctcat tcagcggaat atgctggacg gcacagtact ggtccaccgt aaaatgccag
    11521 ttcagggacc ccatggcccc ccttttcaat gcccgggcat gcgccgcggc atcacccttg
    11581 ggattagcgg tgctgtgaat cgtgataaac cgaggatgca tggaatgggc ggcacggcgc
    11641 gcacgggagc gc
//
LOCUS       DAOMSU010000044        11610 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_302_length_11610_cov_2.952402, whole genome shotgun sequence.
ACCESSION   DAOMSU010000044 DAOMSU010000000
VERSION     DAOMSU010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11610)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11610)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11610
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_302_length_11610_cov_2.952402"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..149
                     /locus_tag="PK077_03305"
     tRNA            76..149
                     /locus_tag="PK077_03305"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:108..110,aa:Gly,seq:tcc)
     gene            345..860
                     /locus_tag="PK077_03310"
     CDS             345..860
                     /locus_tag="PK077_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23356.1"
                     /translation="MADIVQAGLKAGALDMKAVSGVLNDAAKAGVSDHVMGAMVSMAA
                     GAFPSNAPAVASAAVRSYGPGVKEEQVRNVIASAVSSQPHPYASVAPISEAVEKALGN
                     SPVAGTVPSIAVAVAAQTPDNPLSGVTVQEHGLVKPVEDVAEGCLVLPGGLPVGGTPT
                     SPAPVSNPAGN"
     gene            885..2099
                     /locus_tag="PK077_03315"
     CDS             885..2099
                     /locus_tag="PK077_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23357.1"
                     /translation="MKSLFYLSLVAAGMFAVHAHGQSLYTLAGPVGLEESLPLKWFFN
                     LAGGYDDNVNASNYNKQESAFISTDIGCSLANYDAVTQYSFSAKLGGIFYLKDLDGGT
                     NEALSNSTLSAKLSHSFDQTLRYNGSVSFAWQPEPNYSNGIANARRDGDYIYVYVSSS
                     VSKAWTSRYSTTLGANFSMIDYQEDSAKTDNRDYVGMNFTNRYKWTERLAVSLGWTGS
                     YCNREYGNNEFSNFVMAGAEYAVSENTSATLRVGPQFKYVENYGTKTYPSAEFGLNHR
                     LSDRFMLGTFVRYSNEAVNTYIPYNGASYYSNETWRFGVHSTLKLTHRVSLNCGVNLV
                     ASDYTRPSSISNSDTSNLTFNATAGIKLLLTNALALTAQYSYTNGSYSGYNYPMPSYN
                     RNVFSFGMNYSF"
     gene            2192..4399
                     /locus_tag="PK077_03320"
     CDS             2192..4399
                     /locus_tag="PK077_03320"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRN23358.1"
                     /translation="MSAQDQQNSDRIVRNIDYLQVLKNRWKEVFLVFLLVLVISVVVT
                     FLMPPSYRATSQFQIKLPKPVMDIGRGGDVVATSNLTANYIPMQYSVLTSAEVLKVVS
                     RKLNLAAEWGMTDEMAATNLADMIKVAPVRATDLVDVIVSGPDPKLVQNIAKAVPEAY
                     KQDREMRENRLVETAIKSLKDVLREQEDAVNEKMMALKKLIAESSYLPTSWRNGEHRS
                     TLTAGMEEEEFRNAKAQAMDFERREKELASYVQELQRLPDDKLLDYVISSDLLNAETV
                     GADALRKTYAEFSDKLKEKENLKSQNIGARHPKMLALLETERILGDKLNKELIGLRSS
                     LKSKLEMVAASRKHWEATVAQKEEDLQKHVLQVPMYEQASRDYDAALDQLKELDSRYK
                     DEIARLRVPREVIEMYQLPTYPVAPYKPNVTINLAVGAGAGLLLGIGLALLLEFMDTS
                     VKTMEDVERALQVPVLGIIPKNVPILHSSDTMGPDAEAYRILRTNIEFNKKGLEEISL
                     TFVSGSAGEGKTTTLCNLAYICAQGGYATLMIDADLRRSKLHRYYELDNEVGLTSYLL
                     EDYPLEEVIFQTPIENLYVMPAGPTPFDPSGALNSRKFNELLQEVKQRFDIVLVDSPP
                     ILGVSDSAVIVSEVDMTLMVVQPRKLPLKALLRQKQVIESVGGNLAGVVMNNVDITSD
                     HQYQYYTTYYSYYSADGSSSGENADASSMKKVERSGKAKELAATQSHDSEDLY"
     gene            4444..5016
                     /locus_tag="PK077_03325"
     CDS             4444..5016
                     /locus_tag="PK077_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23359.1"
                     /translation="MLGPAGLGSAQSPDAVPPQGTIEIQVRGIPSDDATAISGQYLLD
                     GNGTIRMPQLPPGQDVLRVVGKTARQIEDMLMAAYKKAELYTAPTFLVKVQSSDARLT
                     EHIVLVSGGVAMKKNVLWRRGMTLLEAIVGAGDITDWGSRYVQVTRGGKTVTYDYFSI
                     KDRAIPIQPNDRIFVPQRGIFEGRPAKLLP"
     gene            5592..6125
                     /locus_tag="PK077_03330"
     CDS             5592..6125
                     /locus_tag="PK077_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23360.1"
                     /translation="MIYRFKKTFIGVGGREYISNPKLSFPEHVKIRRRGTNPRSGQPY
                     TEAGSAFVETSAVAARLCCSASAARQYLHRHGVRFRVVRKGTGPLALYWRRLDVERVL
                     AGREPVRRRVPPCYMDMAGAVSMLGVVRSYVYRLVKKGLLREYRCRMKTARGLRVRCF
                     YHREDLEHVRLHVLQKR"
     gene            6191..6784
                     /gene="ruvA"
                     /locus_tag="PK077_03335"
     CDS             6191..6784
                     /gene="ruvA"
                     /locus_tag="PK077_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRN23361.1"
                     /translation="MIAFLRGTVAESYPQRLILDVHGVGYEVIVPLSTFDRLNPLPGR
                     ELTLKTYLHVRENMQVLYGFATDAERDIFLLLIERVSGIGPATAIAILGSLSVEQFKQ
                     AVVGGDVSGIARAKGVGKKTAERIVLELKDKVGLAATWEAQAQGATSQAAGDAELALI
                     ALGFKQVESRKAIAAHLKDHPDADVDELIRAALRSMN"
     gene            7012..8037
                     /gene="gap"
                     /locus_tag="PK077_03340"
     CDS             7012..8037
                     /gene="gap"
                     /locus_tag="PK077_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRN23362.1"
                     /translation="MAKYAINGFGRIGRNVLRAMSKEERNKVVAINDLTPIETIAHLL
                     KYDSTQGKFDGEISIEGDYLVVDGHKILITVERDPANLPWKDLGVDVVLESTGLFTKR
                     DAAKKHLDAGAKKVLISAPSPDPDLTFVLGINDSEYDPAKHDIVSNASCTTNCLAPMV
                     KVLDDKFGVEKGMMSTIHSYTNDQRILDLPHKDPRRARAAAINIIPTTTGAAKAIGEV
                     MPNLKGSLNGASFRVPTPTGSLTDFVAVLKKDVTVEEVNAAMKEAAEGPLKGILAYSE
                     EALVLQDIVSDPHSCIFDSGFTYVVGGNLVKVCGWYDNEWGYSNRAAQAMKKLGDSLG
                     CGCSCGK"
     gene            8205..9416
                     /locus_tag="PK077_03345"
     CDS             8205..9416
                     /locus_tag="PK077_03345"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRN23363.1"
                     /translation="MAKLTVRDLDAKGKEVLMRVDFNVPLKDGEITNDARIVAALPTI
                     KFLLDQGARLVLTSHLGRPKNEPDPAFSLKPVAARLSELLGKDVKFVSAAIGPEAEAS
                     RAAMKDGDVVLLENVRFYPGEKKNDPEFAKSLLGNATLFVNDAFGTAHRAHASTEGVT
                     HFAEKSAMGFLIERELEYLEGKLENPEKPFVVIMGGAKVSDKIEVLSKLMEKADTFLI
                     GGAMANTFLAAEGYDLGASKIEGDKLDLAREILAAAKAKGVKFLLPADVRVAMKFEDG
                     AETFCTAPFAEGGKVPEGGMAIDIGDKAIEEFSAIIKDAKTVLWNGPMGVFEMDCFAK
                     GTKEVAEALADSSAISIVGGGDSVTAAKKFKVQDKLSFCSTGGGASLELLEGKVLPGV
                     GALTDKCCCGK"
     gene            9638..10642
                     /locus_tag="PK077_03350"
     CDS             9638..10642
                     /locus_tag="PK077_03350"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HRN23364.1"
                     /translation="MLPGSLSMMKLLHCFLAVLWWCSPAPAADISAAEKAEYGGYPGR
                     YMLLDSSSPDRQSLSTPVAAYVHDNGMEVALIGAIHVAEPEYYDRLNKLFRRYDVLLF
                     EMIGGEGLRREEELRRKIDRSKPLGGLTLEEAREWNRIVEWRKKCKQEEKSFLMGQLG
                     GVYKKFSDILGLQTQRQGIDYSAAHFVHADMTLDEFRKAQARKGESIAGLMLKSSLSS
                     LVEKTGTNRAGELGLMADFLAGNKTGLKNKLMGMMANAPNGLENTVILEERNAKCMEV
                     FDRWSGKGVRRIGVFYGAAHLPGLHGALLERGYRLREVRWLPAWSTREQGADGQRGEG
                     "
     gene            complement(10648..>11610)
                     /locus_tag="PK077_03355"
     CDS             complement(10648..>11610)
                     /locus_tag="PK077_03355"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HRN23365.1"
                     /translation="VGIWVWVRWSDVNNPANDWENMRSFFSSLSKTGIRGIKIDFMDS
                     ASQERLAFYDAVAENLAKNKLMVNFHGANTPTGEERSWPHEMSREGIYGGEQNIWAAI
                     GGQHYCALPFTRLISGHADFTGGYFGHEPKLRGSSWTLQMAANIIYTSPMLHWVSNPA
                     DMEAAFPKDSPEREVVRNIPSVWEETIVLPPSAIGECAAFARRSGNQWYIALMNGDGK
                     ERTVSIPLNFLDKNTAYQATILRDLAEKNDGWSVETRKVTSGNALSFTMRIKGGGIVR
                     MAPSGTRHPAPGREKEPPLFFPLQRRSGGKPCSSAATPPVSKKE"
BASE COUNT         2519 a         3005 c         3364 g         2722 t
ORIGIN      
        1 tttttttgtt attttgacgc agtaatgaaa aaaaacttgt cttatcccgt gatttgaaca
       61 taatgcgttc gttgcgcggg tatagcttaa tggtaaagct ccagccttcc aagctgatta
      121 tacgggttcg attcccgtta cccgctccat ttttttatta aattcacctg ataaatactt
      181 cctttatgaa agccaggttc gtgacaaagg cggcgcttag tgcgctgaca cttgcgtttg
      241 cgggtaccct tcccggtatg gctcagcaaa ctgcttctcc ggcagccgct ccggctgttg
      301 atcaggttaa agcctccgct gttgaaaggg cttttatgaa gaaaatggca gatattgttc
      361 aggccggatt gaaggccggt gctctggata tgaaagcagt gtccggagtt ttgaacgatg
      421 ccgctaaggc tggtgtatcc gatcatgtga tgggtgcgat ggtatccatg gctgcgggtg
      481 ccttcccgtc caacgctcct gccgttgctt ccgccgctgt ccggagctac gggcccgggg
      541 tgaaggaaga acaggtgcgc aatgtgatcg cttccgctgt ttcctcccag cctcatccgt
      601 atgcatccgt ggctcccatt tcggaagcgg ttgaaaaagc tttgggcaat tctccggtag
      661 ccggtactgt tccttccatt gctgtggccg tggctgccca gactcctgat aaccccctct
      721 caggggtaac tgtccaggaa catggcctgg taaagcctgt tgaagatgtt gccgagggct
      781 gcctggttct gccgggcggt cttcctgtgg gcggcacgcc caccagccct gctcccgtgt
      841 ccaatcctgc tggcaattaa cctgattctt atttaataaa tagcatgaag agtctttttt
      901 atctctccct ggtggccgcc ggcatgtttg ccgtccatgc ccacgggcag tctctgtaca
      961 cgctggcggg tccggtgggt ctggaagagt cccttcctct caagtggttc ttcaacctgg
     1021 cgggagggta tgacgacaat gtcaacgcct ccaattataa caagcaggaa tccgcgttca
     1081 tcagtacgga catagggtgc tccctggcca actacgatgc cgtgacccag tattctttct
     1141 ccgccaagct ggggggtatt ttttacctga aagaccttga tggcggaacg aatgaagccc
     1201 tttccaattc cacgctgtcc gccaaacttt cccatagttt tgaccagacg ttgcgttata
     1261 acggcagcgt ctccttcgca tggcagccgg aacccaacta ttccaacggt attgccaatg
     1321 cccgccggga cggcgactac atttacgtgt atgtttccag ttccgtgagc aaggcgtgga
     1381 cttcccgcta ttccacgacg ctgggcgcca atttctccat gatcgactat caggaggatt
     1441 ccgccaagac ggataaccgc gattatgttg gcatgaattt caccaaccgg tacaaatgga
     1501 cggaacgtct ggctgtttcc ctgggctgga cgggatccta ctgtaatcgc gaatacggca
     1561 ataacgaatt ttccaacttc gtcatggccg gcgctgaata cgcggtgagc gaaaatacct
     1621 ccgccacgct gcgcgtaggt ccgcagttca agtatgtcga gaattacgga acaaaaacct
     1681 atccttccgc ggaattcggc ttaaaccacc gcttgagcga ccggttcatg ctcggaacgt
     1741 tcgtccgtta ttccaacgaa gcggtgaata cctacattcc ttacaacggc gccagctatt
     1801 attccaatga aacatggcgt ttcggggtac acagcacgct gaagctgacg caccgcgtca
     1861 gtctgaattg cggagtaaac ctggttgcca gcgattatac gcgtccgagt tccatcagca
     1921 acagtgacac gtccaacctg actttcaatg ctaccgccgg tatcaagctt ctgctgacga
     1981 atgccttggc ccttacggcc caatattctt acacgaacgg ttcctacagc ggctataact
     2041 acccgatgcc ctcctacaac cgcaatgtgt tctccttcgg catgaattac agcttctaag
     2101 tcttgactga ccattaatgc ctttactcct ctaattgcga attttcattc acaattagag
     2161 gagtttttat cttactgcct tactatattt catgagtgcc caggatcagc agaacagtga
     2221 tcgcatcgta cgcaatattg attatttgca ggttttaaaa aatcgctgga aggaagtctt
     2281 tcttgtcttc cttctggtgc tggtcatttc cgttgtagtg actttcctga tgccgccttc
     2341 ctaccgggcg accagccagt ttcagattaa actgcccaag cctgtgatgg atatcggcag
     2401 agggggggac gtagttgcca cttccaactt gacggccaac tacattccca tgcagtattc
     2461 cgtgctgact tccgccgaag tgcttaaagt ggtatccaga aaactgaacc ttgccgcgga
     2521 atgggggatg acggatgaaa tggcagcgac taatctggcc gacatgatca aggtggcccc
     2581 cgtgcgcgcc acggaccttg tggacgtcat tgtttccggt ccggacccca aactggtgca
     2641 gaatatcgcc aaggcggtgc ctgaggcgta caagcaggac cgtgaaatgc gcgaaaaccg
     2701 tctggtggaa accgccatca agagcctgaa ggatgtcctt cgggaacagg aggatgccgt
     2761 caatgaaaaa atgatggctc tcaaaaagct gattgcggag agttcctacc tgcccacgtc
     2821 ctggaggaac ggtgaacatc gcagcaccct gacggccggc atggaagaag aggaattccg
     2881 gaatgccaag gcgcaggcca tggattttga aaggagggaa aaagaactgg cttcctatgt
     2941 gcaggaattg cagcgtctgc ccgacgacaa gttgctggat tacgtgatca gttccgacct
     3001 gctgaatgcg gagactgtgg gtgcggacgc tctccgtaag acctatgctg aattttccga
     3061 caagctcaag gaaaaggaaa atctgaaatc ccagaacatc ggcgccaggc atcccaaaat
     3121 gctggcattg ctggaaacgg aaagaattct gggggataag ctgaacaagg aattgatagg
     3181 cctgcgttct tccctgaaga gcaagctgga aatggttgcc gcgagccgga agcactggga
     3241 ggctaccgtc gcccaaaaag aggaagatct tcagaagcac gtcctgcagg ttcccatgta
     3301 tgaacaggcc agccgtgatt atgacgctgc gctggaccag cttaaggaac tggactcccg
     3361 atacaaggat gagattgccc gtttgcgcgt gccccgtgag gtgattgaaa tgtatcagct
     3421 gccgacctac cctgttgcgc cctacaaacc gaatgtgact attaacctgg cggtgggagc
     3481 gggcgccggt ctgttgctgg gcatcgggtt ggccctgctt ctggaattca tggatacttc
     3541 cgtgaaaacc atggaagacg tggaacgggc tcttcaggtt cccgtactgg gcattattcc
     3601 caagaatgtg ccgattctgc attcatccga taccatgggg ccggatgcgg aagcctaccg
     3661 cattttgcgg acgaacatag aattcaacaa gaaaggcctg gaggaaattt cgctgacctt
     3721 tgtttccggc agcgccgggg aagggaagac caccactctg tgcaacctgg cctatatttg
     3781 cgcccagggg gggtatgcca ccctgatgat tgacgccgac ctgcgccgtt ccaagcttca
     3841 ccggtattat gagctggata atgaggtagg gctgacttcc tacctgctgg aagattatcc
     3901 tctggaggaa gtcatttttc agacgcccat tgaaaacctg tacgtcatgc ccgcggggcc
     3961 tactcccttt gatccttcag gcgccttgaa ttcccgcaag tttaatgaat tgctgcagga
     4021 agtgaagcaa cgttttgaca ttgtgctggt ggattcccct cccattctgg gggtcagtga
     4081 ctccgccgtc attgtaagcg aggtggatat gactcttatg gttgtccagc cccgcaagct
     4141 gccgctgaaa gccctgttga ggcagaagca ggtaattgag tccgtgggcg gtaacctggc
     4201 cggcgtggtg atgaataatg tggacattac gtccgatcac cagtatcagt attacacaac
     4261 ttactattcc tactattctg cggatggctc ttcctccggc gagaatgcgg atgcctcctc
     4321 catgaagaag gtggaacgtt ctggaaaggc caaggagctg gcagccaccc agtcccatga
     4381 tagtgaagat ttgtattaac ccatggctcc atgaaaaaat atatttgttc catcctttgt
     4441 cttatgctgg gcccggcggg gctgggctcc gcccagtctc cggatgccgt gccgccccag
     4501 ggcaccattg aaatccaggt gcgcggcatt ccttccgacg acgctaccgc tatttccggg
     4561 cagtatctgt tggacgggaa cggtacgata cgcatgcccc agctccctcc ggggcaggat
     4621 gtactgcgcg tggtaggcaa aacggcccgc cagattgaag acatgctgat ggccgcctac
     4681 aagaaggcgg agctgtacac ggcccctacg tttctggtga aggtgcagag ttcggatgcg
     4741 cggctgacgg aacacatcgt tctggtaagc ggcggtgtgg ctatgaagaa gaatgttttg
     4801 tggcggcgcg gcatgacgct tctggaggcg attgtgggcg ccggcgacat cacggactgg
     4861 ggatcccgct acgtgcaggt gacgcgcggt ggaaaaaccg tgacgtatga ttatttttcc
     4921 attaaagaca gggcgattcc catccagccc aatgaccgca ttttcgttcc tcagcgcggg
     4981 atttttgaag ggcgtccggc aaaacttctt ccctgaggca tttttcccat acccggcttt
     5041 ccgcaaggaa gccgggtttt ttattgggcc ggttttcatg atgggggaaa tatcggaaaa
     5101 aacgcggatg gggaaacggg gggcagagtg aaggggcgtg tcgtcagcgg ttggtgaaac
     5161 cggggggctt ctcatgatcc cttcttcagg cttcttgccg gaaatgaatg ttttcgggga
     5221 agccgtatgg tggaatcgcg cagggaaaga agttgtctct tgaaagtttg cggcatcgga
     5281 aaggaacgcc tggaaaaagg ggggaaattt gtttccagcc cctcttgttt atgggaatgg
     5341 aaggcttgag atccggaaaa gattaagggg gataagtttc ccgatgggcg cgtaggccct
     5401 tttcacatta ttggccggca tggtctccgg gcatatattg cggagaaatg agtttagtat
     5461 gaatgtaaaa aacagaaaaa cgttgaacat tcttaaactc aatgcatttt tggaatctcc
     5521 aaaatatgtt agagtttcgt gcggaacggc tgcatgaggc ttcgtaaatc caatgtgaaa
     5581 acccgttaat catgatatac cgttttaaga aaacattcat cggcgtggga gggcgtgaat
     5641 atatcagcaa ccccaaattg agtttccctg aacatgtcaa aatccgccgc cgcggaacga
     5701 atcccaggtc cggccagcct tacacggagg cgggtagcgc atttgtggag acatccgccg
     5761 tcgcggcacg gctctgctgc agtgcttccg ccgcgcgcca gtatctgcac cgccacggtg
     5821 tccgtttccg ggtggtccgc aagggtacgg gccccctggc cctgtactgg cggcggctgg
     5881 atgtggagcg ggttcttgct ggaagggagc ccgtgcgccg ccgcgtgcct ccgtgttata
     5941 tggatatggc cggggctgtc agcatgctgg gagtagtgcg cagctatgtt tacaggctgg
     6001 tgaaaaaagg gctgctgcgt gaatacaggt gcaggatgaa aacagcccgt ggactgcgcg
     6061 tgcgctgctt ttaccaccgg gaggatctgg aacatgtcag gctgcacgtg ctccagaaaa
     6121 gatgacggaa gcggttccgg aggatgcgtt tttagcttgc gcgcgggccg gattgtgtca
     6181 cgttaagggt atgatagctt ttctgcgcgg cacggtggcg gaatcatatc cccagcggtt
     6241 gatcctggat gttcatgggg tggggtatga agtgattgtg cccctgtcca cgtttgacag
     6301 gctgaatccc cttcccggca gagagctgac gctgaaaacc tacctgcatg tccgggagaa
     6361 catgcaggtg ctgtacggtt ttgccacgga tgcggaaagg gatattttcc tgctgctgat
     6421 tgaacgtgtt tccggaatcg gcccggcaac ggcgattgcc attttgggtt ccctgtccgt
     6481 ggagcagttc aagcaggcgg ttgtgggcgg agatgtttcc ggcattgctc gagccaaggg
     6541 ggtgggcaag aagacggcgg aacgcatcgt cctggaactg aaggataaag tgggcctggc
     6601 cgccacgtgg gaagcccagg cccagggcgc tacttcccag gccgccgggg acgcggaatt
     6661 ggcgctgatt gcgctcgggt tcaagcaggt ggaatcccgc aaggcgatag ccgcccatct
     6721 gaaggaccat ccggatgcag acgtggacga gctgatacgc gccgctttgc gttccatgaa
     6781 ttgaaggagc ttccggagga aggaaggtgt ttttgcaagt ttttgatttt catgagggaa
     6841 catgtccgct tggcggagtg ttccctgttt gcggaagctg ggggaacata ctgccatgat
     6901 gggggaacaa tagtgttgac ggctggttga aacgttttta aacttgcagc gtccatggta
     6961 cttgaataag tccgggacta gtaattaatc aaatctgaag ccctcaatct tatggccaaa
     7021 tacgctatta acggttttgg acgcattggt cgcaacgtac tgcgcgccat gtccaaggaa
     7081 gaacgcaaca aggttgttgc catcaatgac ctgactccta tcgaaacgat cgcccacctg
     7141 ctcaagtatg actccacgca gggcaagttt gacggtgaaa tttccatcga gggtgattat
     7201 ctggtcgttg acggtcacaa gatcctcatc accgtggaac gtgatcccgc caaccttccc
     7261 tggaaggatc tgggcgtgga cgtcgttctg gaatccaccg gcctgttcac caagcgcgac
     7321 gccgccaaga agcaccttga cgccggcgcc aagaaggttc ttatttccgc cccctccccg
     7381 gatccggacc tgactttcgt tctgggcatc aacgacagcg aatacgatcc tgccaagcac
     7441 gatatcgttt ccaacgcttc ctgcaccacc aactgccttg ctccgatggt gaaggtgctg
     7501 gacgacaagt tcggcgttga aaagggcatg atgagcacga ttcactccta cacgaacgac
     7561 cagcgcattc tggaccttcc gcacaaggat ccccgccgtg cccgcgccgc cgcgatcaac
     7621 atcattccga cgaccaccgg cgccgccaag gccattggtg aagtaatgcc gaacctgaag
     7681 ggttccctga acggcgcttc cttccgcgtt cctactccga ccggttccct gaccgacttt
     7741 gtggccgtgc tcaagaagga tgtgaccgtg gaagaagtaa acgccgccat gaaggaagcc
     7801 gctgaaggcc cgctgaaggg catcctggct tactccgaag aagcgctcgt tcttcaggac
     7861 atcgtttccg acccccactc ctgcatcttt gactccggct tcacgtatgt ggtcggcggc
     7921 aacctggtga aggtctgcgg ctggtacgac aacgaatggg gttactccaa ccgcgccgcc
     7981 caggccatga agaaactggg cgacagcctg ggctgcggct gctcctgcgg caagtaagtt
     8041 ctttcggaca acgcttgctc ttcagttgca cgacaagtct tgccgtccgc ctgtttcaat
     8101 tttccggctt tcctggccgg ttttgccggg cgggcgggga tttatccggg agggcagaaa
     8161 tgccctccct tttttattca ttgaaccaaa caatataaac cgatatggct aaacttactg
     8221 ttcgcgacct tgacgccaag ggaaaggaag tcctcatgcg cgtggatttc aacgtccctc
     8281 tcaaggatgg ggaaattacc aatgacgccc gcattgtggc tgctctcccc accatcaagt
     8341 tcctgctgga ccagggcgca cgcctcgtcc ttacctctca tctgggccgt cccaaaaacg
     8401 aacccgatcc cgctttttcc ctgaagcccg ttgccgcccg cctttccgaa ttgctcggca
     8461 aagacgttaa attcgtttcc gccgccattg gcccggaagc ggaagcctcc cgcgccgcca
     8521 tgaaggacgg tgatgtggtt ctgctggaaa atgtccgttt ttatcccggt gaaaagaaga
     8581 acgatccgga gttcgccaag tccctgctgg gcaatgccac gctgttcgtg aacgacgcat
     8641 tcggcacggc ccaccgtgct cacgcttcca cggaaggcgt gacgcatttt gcggaaaaga
     8701 gcgccatggg cttcctgatt gaacgtgaac tggaatacct ggaaggcaag ctggaaaacc
     8761 cggaaaaacc ctttgtggtg attatgggcg gcgccaaggt gtccgacaag attgaagtgc
     8821 tttccaaact gatggagaaa gctgatacgt ttctgattgg cggcgccatg gccaatacgt
     8881 ttctggcggc tgaagggtat gacctgggcg cttccaagat tgaaggagac aagctggatc
     8941 tggcccggga aattctggcg gcggccaagg ccaagggcgt gaagttcctg cttcctgcag
     9001 atgtgcgcgt agccatgaaa tttgaagacg gtgccgaaac cttctgcacg gctcctttcg
     9061 ctgaaggcgg caaggtgccg gaaggcggca tggccatcga catcggcgac aaagccattg
     9121 aagagttctc cgccatcatc aaggatgcca agaccgttct gtggaacggc ccgatgggcg
     9181 tatttgaaat ggactgcttc gccaagggaa ccaaggaagt cgcggaagcc ttggcggatt
     9241 cctccgccat ttccattgta ggcggcggcg attccgtgac ggcagccaag aaattcaagg
     9301 tgcaggataa gctttccttc tgttccacgg gcggcggcgc ttccctggaa ctgctggaag
     9361 gcaaggttct tcccggtgtg ggggccttga ccgacaagtg ctgctgcggc aagtaaccgt
     9421 tcatggccgg gcggattgtt ttcgcccggc gttcagaatg ggctttttta ttccctgcgc
     9481 ccgttttcgg gtgcggggat tttttgattt gccggctttc gttgaaaaag cgcgggagtt
     9541 ttgcgattcc ggctttttga agaaggtggg aatccatgct tatgccgtct ttccgttccg
     9601 gaaaggaagg cagtcttttt ttgggcttgc tcttaatatg ctccccggta gtctgagcat
     9661 gatgaaactg ctgcattgtt ttctggctgt cctttggtgg tgttctcccg ctccggctgc
     9721 ggacatttcc gccgcggaga aggcggaata tggcgggtat cccgggcggt acatgctttt
     9781 ggattcttcc tcgcccgacc ggcagagcct gtccactccc gtagctgcct atgtccatga
     9841 caatgggatg gaagtagctc tcattggtgc tattcatgtg gcggaaccgg aatattacga
     9901 ccggctgaat aagctgttcc gcaggtacga tgtgctgctt tttgaaatga ttggcggaga
     9961 gggcctccgg cgggaagaag aattgcgccg caagatagac cgcagcaagc cgctgggggg
    10021 gctgactctg gaagaggcgc gggaatggaa ccggatagtg gaatggagga agaaatgcaa
    10081 gcaggaggaa aagtcttttt tgatgggcca gttgggcggt gtttataaga aatttagcga
    10141 tattctggga ctccagaccc agcgtcaggg cattgattat tccgcagccc atttcgtcca
    10201 tgcggacatg acgctggatg aattccgcaa ggcgcaggcc agaaaaggtg aaagcattgc
    10261 cggcctgatg cttaaatcca gcttgtcctc cctggttgag aaaaccggga cgaaccgggc
    10321 cggggaatta ggattgatgg cggatttcct ggccggcaat aaaacggggt tgaagaataa
    10381 gctgatgggc atgatggcca atgctcctaa tggtctggag aataccgtga ttctggaaga
    10441 acgaaatgcc aagtgcatgg aagtgtttga ccgttggagc ggcaaggggg tgcgcagaat
    10501 aggcgtcttt tacggagcgg cgcatctccc cggcctgcac ggcgctcttt tggaacgcgg
    10561 ctaccgcctc agggaggtgc gctggcttcc ggcgtggagc accagggagc agggtgcgga
    10621 cgggcagcgc ggagagggct gagtttttta ctcttttttc gagacgggcg gtgtagcggc
    10681 ggatgaacat ggttttccgc cgctccgcct ttgaagaggg aagaagaggg ggggctcttt
    10741 ttccctgcct ggggcaggat gccgcgttcc ggagggagcc atgcggacga ttcctccgcc
    10801 tttgatgcgc atggtgaagg aaagggcatt tccggaagtg actttccggg tttccacgct
    10861 ccatccgtca tttttttccg ccagatcccg gagaatggtg gcctggtatg ccgtgttttt
    10921 gtccaggaaa ttcaaaggaa tggaaacggt gcgttccttg ccgtctccgt tcatcagagc
    10981 aatgtaccac tggtttcctg acctccgggc gaaggcggcg cattccccaa tggcagacgg
    11041 cggaaggacg atggtttcct cccatacgga agggatgttc cggacaactt cccgttcagg
    11101 ggaatccttc gggaaagcag cttccatgtc cgcggggttg gagacccagt gaagcatggg
    11161 ggacgtgtaa atgatattgg cagccatttg gagggtccag gaagagccgc gcagcttggg
    11221 ttcgtggcca aagtagcctc ccgtgaaatc ggcgtggccg gatatcaggc gggtgaaagg
    11281 caacgcgcag tagtgctgcc cgccaatggc ggcccagatg ttctgttctc cgccgtaaat
    11341 gccttcccgg ctcatttcgt gcggccagga acgttcctcc cccgtggggg tattggctcc
    11401 gtggaagttg accataagct tgtttttcgc caggttttcc gctacggcgt cgtagaaggc
    11461 caaacgttcc tgagaggcgg agtccatgaa gtctatcttg attcccctga ttcccgtttt
    11521 ggagagtgag ctgaaaaagc tgcgcatatt ttcccagtca ttggcggggt tgttgacgtc
    11581 tgaccatcgc acccataccc agatgcctac
//
LOCUS       DAOMSU010000045        11541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_304_length_11541_cov_2.720791, whole genome shotgun sequence.
ACCESSION   DAOMSU010000045 DAOMSU010000000
VERSION     DAOMSU010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11541
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_304_length_11541_cov_2.720791"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..700)
                     /locus_tag="PK077_03360"
     CDS             complement(35..700)
                     /locus_tag="PK077_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23366.1"
                     /translation="MVHPWTTTSSAPNTCVWRSEYLRLAHRIRKGRRYNAHQLKIQER
                     IGPGQQDDEQPDAHQEGRPEYGRRPGKRVPSQRAFPAIGRKKNSRFLIFRGIPVTPAV
                     QVHSRQHRQRQKNSGGKEQPQKAGHSGKGGRHRRPRHGPKAASAGNQGKQAFHLPRLH
                     DVRQHAPRHGNGKQVKYGQPHIKRTGRPDISRACQHERRENRQVKNEKTVRPRKNGLS
                     PHL"
     gene            699..1130
                     /locus_tag="PK077_03365"
     CDS             699..1130
                     /locus_tag="PK077_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23367.1"
                     /translation="MLLQVFLAGRIVRKFGLAALLAAVPVLISLGFVWMAFAPVFAVV
                     AVVMAVRRIGEYGMVRPGREMLNSVLSPEEKYKAKSFIDTVLYRGGDAVSAWLKSSFN
                     VLGAHSPWAMLAGAAISFLWAATGFLLADRFRKMAGVQGKK"
     gene            1847..>2302
                     /locus_tag="PK077_03370"
     CDS             1847..>2302
                     /locus_tag="PK077_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23368.1"
                     /translation="MKKTLITLIALAGMAHADFVWNGGESITQELWQTESSWSITGSD
                     SWPSAGTGPGTPNSNAWSLISVSGASGSISQLEGWTLKLALQNGADLTVGNVKKFQGG
                     CSIDIDQSSTLTFNSYDGGNDGERTTLNNYGTFNLAYTKSQGGEGFYANL"
     assembly_gap    2303..2312
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2313..2689
                     /locus_tag="PK077_03375"
     CDS             <2313..2689
                     /locus_tag="PK077_03375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN23369.1"
                     /translation="MNLTSSNGSSYNAKIASLTATLTLDGGTASGVTASGHQILTRNL
                     VTLGSNMSFDGTDTSLEMLSGIAGIDSLTAVDDLTNAEVGSYMITKDGNGYSVSYVVP
                     EPAAASLSLLGLAGLAMRRRRK"
     gene            complement(2956..3030)
                     /locus_tag="PK077_03380"
     tRNA            complement(2956..3030)
                     /locus_tag="PK077_03380"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2995..2997),aa:Arg,seq:ccg)
     gene            complement(3183..4295)
                     /locus_tag="PK077_03385"
     CDS             complement(3183..4295)
                     /locus_tag="PK077_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN23370.1"
                     /translation="MEFLIRIASLVRKELLAVLRDKKSRLALIIPPIIQIAIFGYAAT
                     MNVTRVPYAVLDKDGGEAAAQYIANLEGTGIFRRQAAAATEKDIDRMIDNREIVVGLT
                     IPPDFSRSIQTGRPASLQLIADGRNTNTAAIALSYGQQIAAAYGAELLSKNGGSSPVE
                     TESRAWFNPNLITRWFIVPGLIAVLVLINSILSGALSIAREREEGTFDQLLVAPYTPG
                     EILLGKGTASVVTGIMQAVFVVLVAMFWFRIPFQGSIWLLSAALLLFIVTAAAIGLCI
                     SSFAQSLQQAIVGTFLLLVPMVMLSGFATPISSMPEIFQDLTLLNPMRYGLELIQRIF
                     LEGAGFLDLWPLFAAIMAVTVAAVLAAIFSFHHKIS"
     gene            complement(4300..5406)
                     /locus_tag="PK077_03390"
     CDS             complement(4300..5406)
                     /locus_tag="PK077_03390"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN23371.1"
                     /translation="MTASLKRIGALIVKELHQVVRDPGNVGIAVILPAVLLLLFGYGM
                     SMDIKNVRIAYLAVPASSQSTDLETRLTLSRYFQTTRVFSTREAEEKLRTHEADAFIA
                     LQSNAPDTLHNGTTKVQIVVNGVNANQATLIRNYLQAVVGSWASSLGGRTAPVLDVQT
                     RTRFNEANDSHYYLVPGVIVTIMTMIGALLTSLVMAREYERGTLESLFVTPVGSGEIL
                     AAKAATNFLLGMVSLAISMLFAAFVFGIPIRGSLTLLLAVSALFLIVALGLGLVISTA
                     TKNQFLACQFAIMGTFMPALMLSGFLYDILNMPPAVRAITCIIPARYYVTLLQTLFLA
                     GDIPSVIIPCCITLGVFAVVLMGIARMKAPKSLE"
     gene            complement(5403..>6813)
                     /locus_tag="PK077_03395"
     CDS             complement(5403..>6813)
                     /locus_tag="PK077_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN23372.1"
                     /translation="AAERRRRIDDLTQATGLQKFLQRPAGKLSGGMKQKLGLCCSLVS
                     SPPLILLDEPTVGVDPLSRRELWSILKQFSGEEGVGVLVSTSYMDESAYCNRTLIMYE
                     GRLLMDAPPADVIARAEGMCVTVRTPEGLHARQFQSRLAAVPGIINATPQGNTVRIIV
                     PHGHPARKKLEEYHPAPAQPDFSDGFMTLLADQVDLAPQDIPAPAGTPEPERTENGGT
                     VIRVTDLVRKFGSFIAVNHVSFSVRKGQVFGLLGPNGAGKSTTFRMLCGLLPASGGTL
                     NVAGADLRTAAARARRKVGYVAQKFSMYGMLTTRQNLEFFAGAYGMSGKERQEAIRSM
                     EEEFHLTPYMNAPAAHLPGGYKQRLSMACALLHSPDILFLDEPTSGADPLARRDFWLR
                     INALAEKGVTIIITTHFLGEAEFCDNMLIMMDGTTLAEGSPDDIRKHAPPREDGAPAS
                     LEDAFLAITEEHMKKGGET"
     assembly_gap    6814..6823
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6824..7181)
                     /locus_tag="PK077_03400"
     CDS             complement(<6824..7181)
                     /locus_tag="PK077_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN23373.1"
                     /translation="MTTESQPLIDCRNVRKIFPDSAGVPFAAVDDVSFRLSAGEIVGL
                     LGPDGAGKTTLIRLITGLMKPHGGSISVLNLDSVKKSRSIQASIGYMPQKFGLYEDLT
                     VRENMELYARMHGVYGQ"
     gene            complement(7178..8197)
                     /locus_tag="PK077_03405"
     CDS             complement(7178..8197)
                     /locus_tag="PK077_03405"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRN23374.1"
                     /translation="MKKLVILLVLLAAAGTAAWLICRKTPSGPEDKAVLYGNVDLRQV
                     DLAFLISERIDSVLVDEGDTVVPGQKLATLETVRLRQAADEARQIAEAARQNYLRVKN
                     GPRAEEIAQARANVQAAEATLNNAGMRSKRLEALAETKSISRQEADDAVTSRQVAANL
                     DVARKQLELLLAGSREEDVAQALAQYNQAKASLTIREQNLKDAVLYAPGNGVVRNRIL
                     EKGDMASPQKPVYNISLNHTKWVRAYLTESQLGKVKPGFSATVRNDSFPDTGFKGTVG
                     FISSVAEFTPKNVETPDLRTALVYEVRIIVDDPDNRLRLGAPATVTIPLDQNAGTQTP
                     EQPRP"
     gene            8331..11159
                     /gene="alaS"
                     /locus_tag="PK077_03410"
     CDS             8331..11159
                     /gene="alaS"
                     /locus_tag="PK077_03410"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRN23375.1"
                     /translation="MMTATEIRQSFLDFFREKQHTVVPSASLMPQSPGLLFTNAGMNQ
                     FVPYFLGVWTPPWTPARATDTQKCIRAGGKHNDLEDVGYDSYHHTFFEMLGNWSFGDY
                     FKKEAIRWAWELVVERWGFPAERLYATVYAPDKSKGDPGEFDREAWDFWAELFRSRGL
                     DPDVHIVHGNVKDNFWMMGETGPCGPCSELHVDLTPEGNTKGSLVNKDSDQCIEIWNL
                     VFIQYNAESDGSMRNLPACHVDTGMGFERACSIMQCTNGFKDFSRKPSNYATDVFRPL
                     FDRLEVLSGRKYADVYPAPGSKRVDAEDGTLQEAIAFRVIADHLRTLSFSIADGILPG
                     NNGRNYVLRRILRRAVRYGRRLGFTQPFLAELVDTLVESFGQVFPELAARAATVKEVL
                     NREEASFNETLDRGLELFDAETASAGKVSGEFAFKLYDTYGFPIDLTALLAEERGLDI
                     DMERFNRLMEEQRERARAARKSEVVRALDLKTDAVTEFTGYDVDECAATVLEVSRQGD
                     SLFIITDKTPFYAEMGGQVSDAGLIEIGGESYHVMAVQQIGNARAHVVEARPGLEVRP
                     GDRVHLSIDAERRRRIEAHHTATHLLHCALHQVVSPDAAQQGSFVSEDRLRFDFNSSA
                     VSPDQLRLIEEKVNGWIEESLPVHCTERAYADVKGNAAIAQFFGDKYGDVVRVVQVGG
                     CRDGLDGVSMEFCGGTHIANTKNIGLFKIKSEGAIASGVRRIEAMTGDAALEMIRQHV
                     VAKSLEIAKAVEKIKEVNYELADMGLEQVPVPTIEGKPGLTALGASDIRTVNDSLARF
                     DASVEHFKQTALDAEKKLKKARAGQSAAKADALLNEWLSDAPSSLIQVAEGAGELLQE
                     LLNGLKKRQYAGAAFLLCVDSSSLLLGAYCGKDAIADGLSAGDMIREVAALAGGKGGG
                     RADQARGSAPQDADPQALAAAARNIING"
     gene            11192..>11541
                     /locus_tag="PK077_03415"
     CDS             11192..>11541
                     /locus_tag="PK077_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRN23376.1"
                     /translation="MDKKIFPDEPEIPILPNLFTAGNLVCGFFAILTIFEGINQADSD
                     AVAAFSYYQNATFLIFAACLFDLFDGRIARMRGQDGPFGREFDSLADIVSFGIAPALL
                     VAKAVLFQLSPPEVG"
BASE COUNT         2219 a         3024 c         3726 g         2552 t
ORIGIN      
        1 catagggggc cagtttttat tcggatgggt ttcctcaaag gtgcggagaa aggccattct
       61 tccgtggacg tacggttttt tcattcttaa cctggcggtt ttcgcggcgc tcgtgctggc
      121 atgcccggga aatatctgga cggcccgttc gttttatgtg tggctgtccg tatttaacct
      181 gcttaccgtt tccgtggcgt ggagcgtgct gtcggacgtc atgaagccgg ggcagatgaa
      241 acgcctgttt gccctggttg ccagcggaag cagccttggg gccatggcgg ggccggcggt
      301 gacggccgcc cttgccggag tggccggcct tttgtggctg ttccttgccg ccgctgttct
      361 tctggcgctg gcgatgctgg cgggaatgta cctgcaccgc tggcgtgacg ggaattcccc
      421 ggaagatgag gaaacgggag ttcttcttcc ggccgattgc cgggaacgcc ctctgggagg
      481 gaacccgttt gccggggcgt ctgccgtatt ccggtctccc ttcctgatgg gcatcgggct
      541 gttcatcatc ctgctggccg ggaccaatac gttcctgtat tttgagctga tgagcgttgt
      601 agcgtcttcc tttcctgatc cggtgcgcca gacgcaggta ttcggagcgc cagacgcagg
      661 tattcggagc gctggacgtt gttgtccagg ggtgtaccat gctgcttcag gtatttctgg
      721 cgggacgcat tgttcggaaa ttcggattgg cggcgttgct ggcggctgtc cccgttctta
      781 tttccctggg ttttgtatgg atggctttcg ctccggtatt tgccgtcgtg gccgtggtga
      841 tggcggtacg ccgcatcggg gaatacggaa tggtgcgtcc cggccgtgaa atgctgaatt
      901 ccgtattgtc cccggaagaa aaatataaag ccaagagttt tattgatacg gtcctttacc
      961 gtggcggcga cgccgtcagc gcatggctga aaagctcctt taacgtgttg ggtgcccatt
     1021 ccccgtgggc catgctggcc ggagccgcca tttcctttct ttgggcggcc accggattcc
     1081 tgcttgcgga caggttccgg aaaatggccg gagtacaggg aaagaaatga tccttatggc
     1141 ggggaatttt ccgttttgtc cggatacggc ccgccagatg cggcaattcc tggtgatgcc
     1201 tcttttccgg cggctgccgc cgtcagaggt gaactgctgg ggtattgggg aatctggcgc
     1261 gttttgaaaa agcgtggcaa agccgtgttc tgtggaggaa taagtgccag gccttccgtt
     1321 gagggatagt tgcaggcagg ggattgcgtg atgcccttga tggcggaagc ggcgggaggc
     1381 aagacagatg tttttctccg gcgatgaata atttctggaa tgttctttat ttgacggcgg
     1441 tttccctctg cggcggcaac atgcggattt tcaacccgga atcaatacta aatattgtgg
     1501 aaaaataatt taagtatcat ttcaaccctg ttttcaggat gatttttcct ccaaaaaaac
     1561 tccctgccgt ggcgggcatt tcctgttgtt tttttggaaa caattttgac accaatatca
     1621 cattttaaca aaatgtaata tttttaatat aaaaaagtta ctttaaaaat aaagattaat
     1681 ttccccatgc cgcaagacat ctgccacatg caaccctcca tccactccgg cacatgaaac
     1741 ggattgaaaa tccgttattg aagcccaagc ctctgtgagg caagtatgct ggtcatcttt
     1801 ccgttagatg attccgtcat tactattgtt atatattcct ctgataatga agaagacact
     1861 cattacgctc attgctctgg cgggaatggc tcatgccgat tttgtatgga acggcggtga
     1921 gagcattacc caggagcttt ggcagacaga atccagctgg tccatcaccg gttcagattc
     1981 ctggccgtcg gcaggaaccg gtcccggcac gcccaattcc aacgcatggt ctcttatttc
     2041 cgtttccggc gcgagcggca gcatttccca gctggaaggc tggacattga agcttgccct
     2101 gcaaaacggt gcggatttga ctgtagggaa cgtaaaaaaa ttccagggcg gctgttctat
     2161 tgatattgat caatcatcta cgctgacctt caacagttat gacggcggca acgatggtga
     2221 gcgcacgacg ttgaacaact acggcacgtt taacctggct tataccaaga gccagggcgg
     2281 ggaaggattt tacgccaatt tgnnnnnnnn nntcatgaac cttacttcct ccaacggtag
     2341 ttcatacaat gccaagatag caagtctgac cgctacgctg actcttgacg gaggcaccgc
     2401 cagcggagta actgccagcg gtcatcaaat cctgaccagg aacctggtta ccctgggaag
     2461 caatatgtct tttgacggca cggacacttc cttggaaatg ctcagcggca ttgcaggcat
     2521 tgattccctc accgctgtgg acgatttgac taatgccgaa gttggttctt atatgattac
     2581 caaggatgga aacggataca gcgtttccta tgtggttccg gaacccgcag cggcttccct
     2641 gagcctgctg ggcctggctg gcctggcgat gcgccgccgc cgcaagtaac ggcaaacttg
     2701 gattcctgtt tccattcagc tcccttccgc ggttaacgga agggagtttt tttattattt
     2761 cccggaaagg cggcgtattt ccggaagatg tccgggcgga acgggagaga gaagccggga
     2821 gagtgcatat gcatgggcct attcttcccc ggaggatgcc gtaaccggat tagccccggc
     2881 tattgttctg caacgctgca agaaaggaaa aaggcctgtt attgccggga gtaaccactt
     2941 tctttcaaaa aaaagtggta ggcccggtag gaatcgaacc tacatcgtcg gtaccggaaa
     3001 ccgatgtcct atccattgaa cgacgagccc atgttcccaa ggaacgagag aaatagaata
     3061 gaaaaaacgg gccggatgca agtattttta tgccggtgac gggattggca tgatgaaaat
     3121 gtgaaacggg gaagttacgg gcagggaggt ttccggatat tccggggaga aggaaggggg
     3181 gatcaggaga ttttatgatg aaaagagaag atggctgcca ggacggccgc cacggtaacg
     3241 gccatgatgg ccgcgaaaag cggccagaga tccaggaatc cggctccttc cagaaagatg
     3301 cgctggatga gttccaggcc gtatctcatg ggattgagca gcgtgaggtc ctggaagatt
     3361 tccggcatgc tggagatggg cgtggcaaag ccggagagca tgaccatggg caccagcagc
     3421 aggaaggttc ccacaatggc ctgctggagg gattgagcaa aggaggagat gcacagcccg
     3481 atggccgccg ccgtgacgat gaagagcaga agggcggcgg agagcagcca gatggagcct
     3541 tggaagggga tgcggaacca gaacatggct accagcacta cgaagacggc ctgcatgatt
     3601 ccggtgacga cggaggctgt tcccttgccg agcaggattt cccccggggt gtatggggcg
     3661 acgagaagct ggtcgaaggt gccttcctcc cgttcgcggg cgatggagag agctccggag
     3721 aggatggagt tgatgagcac gagcacagca atgaggccgg gaacgatgaa ccagcgtgtg
     3781 atgaggttgg ggttgaacca tgcgcgggat tccgtttcca ccggggagga gccgccgttt
     3841 ttggagagca gttccgcccc gtaggcggca gcgatttgct ggccgtagct gagggcgatg
     3901 gccgccgtat tcgtgttgcg cccgtccgca atgagctgga gagatgcggg cctccccgtc
     3961 tgaatgctgc gggagaaatc cggcggaatg gtgagcccca cgacgatttc ccggttgtct
     4021 atcatgcggt caatgtcttt ttccgtggcc gcggcggcct ggcgccggaa gattcccgtg
     4081 ccttccaggt tcgcgatgta ttgggcggcc gcttctccgc cgtccttgtc cagcacggcg
     4141 tacggcacgc gggtgacgtt catggtggcg gcatagccga agatggctat ctggatgatg
     4201 ggagggatga tgagggccag ccggcttttt ttgtccctga gcacggccag cagttctttt
     4261 ctgacgaggg aggctattct gatcaggaac tccatggcgc tattcgaggg atttgggtgc
     4321 tttcatcctg gcgattccca tgagcaccac ggcaaagacg cccagcgtga tgcagcaggg
     4381 aatgatgacg gaagggatgt ccccggccag gaagagggtc tggagcaggg tgacgtaata
     4441 gcgggccggg atgatgcagg tgatggcccg gacggcgggt ggcatgttca ggatgtcata
     4501 caggaagccg gacagcatga gggccggcat gaaggtgccc ataatggcga attggcaggc
     4561 caggaactgg tttttggtgg cggtggagat gaccagcccc aaccccaggg ccacaatcag
     4621 gaacagggcg gaaaccgcca gcagcagagt gagggagccg cggatgggga tgccgaagac
     4681 gaaggcggcg aagagcatgg agatagccag gctgaccatg cccagcagga agttggtggc
     4741 ggccttggcc gccaggattt ctccgctgcc cacgggcgtg acgaagaggc tttccagcgt
     4801 gccgcgttca tattcccgcg ccatgaccag ggaggtgagg agcgccccga tcatggtcat
     4861 gatggtgacg atgacgccgg gaaccaggta gtagtggctg tcgttggctt cattgaagcg
     4921 ggtgcgcgtc tgcacgtcca gcacgggggc tgtccgcccg cccagggaag acgcccagga
     4981 gccgacgacg gcctgaaggt agttgcggat gagggtggcc tggttggcgt tgactccgtt
     5041 gacgacaatc tggactttcg tggttccatt gtggagcgtg tccggcgcgt tgctttgcag
     5101 ggcgatgaag gcgtccgctt catgggtgcg cagtttttct tcagcctccc gggtagagaa
     5161 gacgcgcgtg gtctggaaat atctggaaag ggtaagcctt gtttccaggt ccgtggactg
     5221 gctggaggcg gggacggcca ggtacgcgat gcgcacgttt ttgatgtcca tgctcatgcc
     5281 gtagccgaag aggagcagga gcacggccgg aaggatgacc gcaatgccca cattgccggg
     5341 gtcccggacc acctggtgga gttccttgac gatgagggct cctattcttt tgagggaggc
     5401 ggtcatgttt ctcctccttt tttcatgtgt tcttccgtga tggccaggaa ggcgtcttcc
     5461 agagaagcgg gggctccgtc ttcacgcggg ggagcgtgtt tgcggatatc gtccggggaa
     5521 ccttccgcca gcgtggttcc atccatcatg atgagcatgt tatcgcaaaa ttccgcttct
     5581 cccaggaagt gcgtcgtgat gatgatggtg acgccttttt ccgccagggc gttgatgcgc
     5641 agccagaagt cgcgccgggc cagggggtcc gctccggaag tgggttcgtc cagaaaaagg
     5701 atgtccgggg agtggagcag ggcgcacgcc atgctgaggc gttgcttgta gccgccgggc
     5761 aggtgcgcgg cgggggcgtt catgtacgga gtcaggtgga attcttcttc catggagcgg
     5821 atggcttcct gccgctcctt tccggacatg ccgtaggctc cggcgaagaa ttccaggttt
     5881 tgacgcgtgg tcagcatgcc gtacatggaa aatttctggg ccacgtatcc aactttcctg
     5941 cgcgctctgg cggcggcggt ccgcagatcc gccccggcca cattgagcgt gccgccgctg
     6001 gcgggcagca gcccgcagag catgcggaaa gtggtgcttt tccccgctcc gttggggccc
     6061 agaaggccga agacctgccc tttgcggacg gagaagctga cgtggttgac ggcaatgaaa
     6121 ctgccgaatt tcctgacgag gtccgttacc cggatgacgg tgccgccgtt ttccgtccgc
     6181 tccggttccg gcgttccggc aggggcgggg atgtcctgcg gggcgaggtc cacttggtcg
     6241 gcaagcaggg tcatgaagcc gtcagagaag tccggctgcg cgggggcggg gtggtattcc
     6301 tccagctttt tccgtgcggg gtggccgtgg ggaacgatga tgcggacggt gttcccctgc
     6361 ggggtggcgt tgatgatgcc gggcacggcg gccagcctgc tctggaattg gcgcgcatgc
     6421 agcccctccg gcgtgcgcac cgtcacgcac atgccttccg cccgggcgat gacgtcggcg
     6481 ggaggggcgt ccataagcag gcgcccttca tacatgatga gggttctgtt gcagtaggcc
     6541 gattcgtcca tgtaggaggt gctgaccagc acccccacgc cttcctctcc ggagaattgt
     6601 ttcaggatgc tccacagttc ccgccgggag agcgggtcca cgcccacggt gggttcatcc
     6661 agcaggatga ggggcgggga ggagaccagg gaacagcaca ggcccagctt ctgcttcatg
     6721 ccgccggaga gcttgcccgc cgggcgttgc aggaatttct gcagcccggt ggcctgggtc
     6781 aggtcgtcga tgcggcggcg gcgctcggca gcgnnnnnnn nnncctggcc gtaaacgccg
     6841 tgcatgcggg cgtaaagctc catattttcc cggacggtaa ggtcttcata aagcccgaat
     6901 ttctgcggca tgtagcctat ggaggcctgg atggaccggc tttttttcac ggagtccaga
     6961 ttcaggacgg agatagaccc gccatgcggt ttcatcaatc ccgtgatgag gcggatcaga
     7021 gtggtttttc ccgctccgtc cggccccagc agccctacga tttccccggc ggacaggcgg
     7081 aaggagacgt cgtccaccgc cgcaaagggg actccggcgg agtccggaaa tattttccgg
     7141 acgttccggc agtcaatcag gggctgggat tccgtggtca tggcctgggc tgttccgggg
     7201 tctgtgttcc ggcgttctgg tccaggggga tggtgacggt ggcgggggct cccaggcgca
     7261 gccggttgtc cggatcgtcc acaatgatgc gcacttcata gaccagtgcc gttctgagat
     7321 ctggcgtttc cacgttttta ggggtgaatt ccgctacgga ggagataaac cccaccgtcc
     7381 ctttgaagcc ggtgtccggg aagctgtcat tgcgcacggt ggcggaaaag ccgggcttta
     7441 ctttgccgag ctgggattcc gtgaggtagg cccgcaccca tttggtatgg ttcagggaga
     7501 tgttgtaaac gggtttctgc ggggaggcca tgtccccctt ttccagaatg cggttgcgca
     7561 ccacgccgtt gccgggggca tagagcacgg cgtctttcag gttctgctcc ctgatggtca
     7621 ggctggcttt ggcctggttg tactgggcca gcgcctgggc tacgtcttcc tcacgggaac
     7681 cggccagcag cagttccagc tgttttctgg ctacatccag attggcggcc acttgccggg
     7741 aggtgacggc gtcgtccgcc tcctggcggg agatggattt tgtttcagcc agcgcctcca
     7801 gccttttgct gcgcattccg gcgttgttca gcgtagcttc cgcggcctga acgtttgccc
     7861 tcgcctgggc tatttcttcc gcgcgcgggc cgtttttaac gcgcaggtag ttttggcggg
     7921 cggcttccgc tatctgccgg gcttcgtccg cggcctgccg gagccgcacc gtttccagcg
     7981 tggcgagctt ctggccggga acaaccgtgt ccccttcgtc caccagcacg gagtcaatac
     8041 gttcggagat gaggaaagcc agatccacct ggcgcaggtc cacattgccg taaagaacgg
     8101 ctttgtcctc gggcccggac ggagttttcc ggcagatgag ccaggcggcc gttcctgccg
     8161 ccgcaaggag aaccaggagg ataaccagct ttttcatagt tttatttata cgcgtgtata
     8221 atttattgca agtaaaaatg gagcccgcat gacggagaag gaattagcga tttactaacg
     8281 gagggagaac aggcataatc cggcgtcttt tacttttccc attatctgtt atgatgaccg
     8341 ccaccgagat acgccaaagc tttctggact ttttccgcga aaaacagcac acggtcgtgc
     8401 cttccgcttc tttgatgccc cagagccccg gtttgttgtt tacaaatgcc ggcatgaatc
     8461 agtttgtccc gtatttcctg ggcgtatgga ctcccccgtg gacgcccgcc cgcgctacgg
     8521 atacccagaa gtgcatccgc gcaggcggca agcacaatga cctggaggat gtggggtatg
     8581 actcctacca ccacacgttt tttgaaatgc tggggaactg gtccttcggg gattatttca
     8641 agaaggaagc tatccgctgg gcctgggagc tggtcgtgga gcggtgggga tttccggcgg
     8701 aacgcctgta cgccaccgtg tacgcgccgg acaagagcaa gggcgacccc ggagagtttg
     8761 accgggaagc ctgggatttc tgggctgagc tgttccgttc ccgagggctg gacccggacg
     8821 tgcatatcgt gcacgggaat gtgaaggata atttctggat gatgggggaa accggcccct
     8881 gcggcccctg ttccgagctg cacgtggacc tgaccccgga ggggaatacg aagggaagcc
     8941 tggtaaacaa ggattccgac cagtgcatag agatatggaa cctggtgttt atccaataca
     9001 atgcggagag cgacggctcc atgcgcaatc ttccggcatg tcatgtggat accgggatgg
     9061 ggtttgagcg cgcgtgctcc atcatgcagt gcacgaacgg attcaaggat ttttcccgca
     9121 aaccgtccaa ttacgccacg gatgtattcc gccccctgtt tgaccgcctg gaagttttga
     9181 gcggacggaa gtacgcggac gtgtatccgg cgcccggttc caaaagggtg gatgcggagg
     9241 acgggaccct tcaggaggcg attgccttcc gcgtgattgc cgatcatctg cgcacgctca
     9301 gtttttccat tgcggacggc attctgccgg gcaacaatgg ccgtaattac gtgctgcgcc
     9361 gcattctgcg ccgtgccgtg cgctatgggc gccgcctggg ctttacccag ccgtttttgg
     9421 cggaactggt ggatacgctg gtggagtcct tcggacaggt gttcccggaa ctggccgccc
     9481 gcgccgctac cgtgaaggag gttttgaacc gtgaagaggc cagttttaat gagacgctgg
     9541 accgcggcct ggaattgttt gacgcggaaa cggcttccgc cggaaaggtg agcggcgagt
     9601 tcgccttcaa gctgtatgat acgtacgggt tccccattga cctgaccgcc ctgctggcgg
     9661 aggaacgcgg cctggatatt gatatggagc ggttcaacag gctgatggag gaacagcggg
     9721 aacgcgcccg ggccgcccgc aagagcgagg tggtgcgcgc cctggatttg aagacggacg
     9781 ccgtgacgga atttacggga tacgatgtgg acgaatgcgc cgctacggtg ctggaagtga
     9841 gccgccaggg ggattccctg ttcatcatca cggacaagac tccgttttac gcggaaatgg
     9901 gcgggcaggt atccgatgcc ggtttgattg aaatcggcgg ggaaagctac catgtgatgg
     9961 ccgtccagca gatagggaat gcccgagccc atgtggtgga ggcccgtccc gggctggagg
    10021 tgaggcccgg cgaccgcgtg catttgagca ttgacgcgga acgccgccgc cgcattgagg
    10081 cgcatcacac cgccacgcat cttcttcact gcgccctgca tcaggtggtc agcccggatg
    10141 cggcccagca ggggtccttt gtttcggaag accggctgcg ctttgacttt aacagcagcg
    10201 ccgtttctcc ggatcagctc cgcctgattg aagagaaagt gaacggctgg attgaggagt
    10261 ctcttcccgt gcactgcacg gaacgcgcct atgcggacgt gaagggcaat gccgcgattg
    10321 cccagttttt cggcgacaag tacggggatg tggtgcgcgt ggtccaggtg ggcggatgca
    10381 gggatgggct ggacggggtt tccatggaat tctgcggcgg aactcatatt gccaatacga
    10441 agaatatcgg cctgttcaag attaagagcg agggtgccat cgcttccggc gtgcgccgca
    10501 ttgaggcgat gactggggac gctgctctgg aaatgatacg gcagcatgtt gttgccaaga
    10561 gcctggaaat cgccaaggcg gtggagaaga tcaaggaagt caattacgag ttggcggaca
    10621 tggggctgga acaggtgcct gtccccacga ttgaaggcaa gccggggctg acggccctgg
    10681 gggcttccga tatccggacg gtaaatgatt ccctggcgcg tttcgacgcc tccgtggagc
    10741 atttcaaaca gacggctctg gatgcggaga agaagcttaa aaaagcccgc gccgggcagt
    10801 ccgccgccaa ggcagacgcc ctgctgaatg agtggctttc cgatgccccc tcttccctga
    10861 tccaggtggc ggagggcgcc ggggaattgc ttcaggagct gctgaacggg ttgaaaaagc
    10921 gccagtatgc gggcgccgcc ttcctgttgt gcgtggacag ttcttccttg ctcctgggcg
    10981 cttattgtgg caaagatgcc attgcggacg gattgtccgc cggagatatg atccgcgagg
    11041 ttgccgctct tgccggaggc aagggaggcg gtcgtgcgga ccaggcccgc ggttccgctc
    11101 cgcaggatgc cgatcctcag gccctggctg ccgccgcccg caatattatt aacggataaa
    11161 ccccttacct gggccctatc ggacaccaat catggacaag aagattttcc cggacgaacc
    11221 tgagattccc attttgccta atttgtttac ggcagggaac ctggtgtgcg gttttttcgc
    11281 cattctgacg atttttgagg gaatcaacca ggcggacagc gacgctgtgg ctgctttttc
    11341 ctattatcag aatgccactt ttttaatctt cgcggcatgt ttgtttgact tgtttgacgg
    11401 ccgtattgcc cgcatgcgcg ggcaggacgg cccgttcggg cgggaattcg attctctggc
    11461 ggatattgtc tccttcggca ttgccccggc gctgctggta gccaaggccg tcctgttcca
    11521 gctgtcccct ccggaggtgg g
//
LOCUS       DAOMSU010000046        11456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_309_length_11456_cov_2.820279, whole genome shotgun sequence.
ACCESSION   DAOMSU010000046 DAOMSU010000000
VERSION     DAOMSU010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11456
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_309_length_11456_cov_2.820279"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1334
                     /locus_tag="PK077_03420"
     CDS             <1..1334
                     /locus_tag="PK077_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HRN23377.1"
                     /translation="GDRYVFTERQVNSILDLRLYQLTALENDKISGEYAELLVRIKDY
                     LDILANESRVLGIVKDELKAIKDKYATPRVTRIIPFSGDMAIEDLIPNDTMIVTITHS
                     GYIKRTNSAEYRVQARGGKGVKAATTRGAKKDAEADFIEHLFAAQNHDYLMFFTNTGR
                     VYVDRVYEIDEAARSAQGRNIKNLLDLQPEEAIAAILRLERRVDEKGNDITFAEGSGN
                     VVFATKDGTVKKTSLNDFANYRKAGIIAINLEEGNSLVNAELTSGADEIVLVTHDGMS
                     IRFPEEDLRTQGRNTIGVRGIRPRAGDYVVALAIVDPQKTLLVASENGLGKRTSFDEY
                     RTQGRGGTGIKTMNCTDKTGKVVSATVVSDEDELMLMTTAGQSVRIKVATVRETGRAT
                     QGVKLMTLNEGESIQDISIVIPDDEDEEAPAETEDAGVGEVSGEMEAPAEE"
     gene            1394..2026
                     /locus_tag="PK077_03425"
     CDS             1394..2026
                     /locus_tag="PK077_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HRN23378.1"
                     /translation="MEDPLSLEQCAGRLERRGFRSFVVPGLREAAEVMRGLVREFRPS
                     SASYGDSMTLKAAGILDDLHSNPDIQFYDGFVPGMVREEKWEIRRQGMTADLFLTGVN
                     AVSMEGTLHWVDMVGNRVAPVAFGPRRVILLAGSNKLVATPAEARERIRRIAPLNARR
                     HEGFRTPCMHTGICADCNSPDRLCNIHMSIVKCFPKGRISVILMEEPGGL"
     gene            2030..3301
                     /locus_tag="PK077_03430"
     CDS             2030..3301
                     /locus_tag="PK077_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HRN23379.1"
                     /translation="MPFQNRIEIMAPAGSFESLAAALQGGADSVYFGVGKLNMRSRAT
                     VNFSEEDLPEIVERCHEAGAKAYLTLNIIVYDEELEAVHALCDAARKAGVDAVIASDL
                     AVISYARSIGLEVHMSVQANVCNMASVKFYAQYADVVVLARELTLAQIRHIIESIRKE
                     GVKGPSGELLRVEIFAHGALCVAVSGKCHMSLAAYNSSANRGACFQNCRRAYRVTDEE
                     TGNELVIDNKYVMSPKDLCTIPVLDQLLDAGVSVLKLEGRGRSSDYVRTVTSVYREAA
                     RACQDGTFSADRAEAWMKRLESVFNRGFWQGGYYLGVKWGEWSGSANSRAVLLKIHIA
                     RVENFYKKNGVAALFLEAGGLSAGQTILITGPTTGAVRMEVAAMRRETAEGMEPVKAA
                     QKGETVYLAVPEQVRRRDKVYLLRPRMLEDA"
     gene            complement(3370..3642)
                     /locus_tag="PK077_03435"
     CDS             complement(3370..3642)
                     /locus_tag="PK077_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23380.1"
                     /translation="MIRISRPEAKREFQKTPAEIIAGVEEEPGGMKLHEVVRLINDIA
                     ARYEVAALTIAEPMPRLTIKIRNMLSQLPLLKPDAPPAQPGRKRQN"
     gene            3745..7290
                     /locus_tag="PK077_03440"
     CDS             3745..7290
                     /locus_tag="PK077_03440"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRN23381.1"
                     /translation="MPDYISFLRLFSCSCALLCAGMLHASGLQPWLRELAVIPPAPSA
                     KEKDAWMRQVWTRTAEYAASLSEPASADDCGDAMVLLVPVFQAWPDGKAAGAALAEIL
                     SVPAESIGDVSSWDDLVKHQEAIQERVRFLRLKKLAAYYDSAAIRRAVKRNRDKYGER
                     YAGAEGFLARLDEWEKELGPLDRWVEQAGPGQAALLREMVDLRRKALIEALPEQNGLK
                     EWVSVRRFNPFGKSSFNHDRPANWQGISSMPGPGRRYRSVIVKFNGVSSSSPVEQLLA
                     DDRWVGHLDIDFSGEKLMFTGNGFGKKENRPWDVFELDLKTGKKESLTAHMPADTDSY
                     NSCYLPDGRIIFVNTSGMQGVPCVAGVDYVGNLHLYDREKKTTRRLTFDQDNNWFPTM
                     LSDGRVMFLRWEYTESAHYFSRVLMHMNPDGSDQKEYYGSNSYWPNSLFNARPLPGRP
                     GMFAGIVSGHHGVKRLGELVLFDVNRGRAATEGAVQKIPGYGKPVENVTKDQLVQGLK
                     TPYFAEPYPLNDECFLAVSSPSGSQGVTNVVWCDIYDNIVPLTASSYFVYADPAPLGP
                     RKKPPVLHDRVKTESKTATVYISDVYRGRAMAGVPRGEARALRVFMSEYSPRNTGSHY
                     AMGMESNWDLKVLYGTVPVNPDGSAIFTAPACQPLTLQVLDGEGRALALMRSWFTAMP
                     GETLSCIGCHERQNCAPPARAVMASRQKPGSIVPWYGPARTFSFMNEVQPVLDRHCIR
                     CHTAEGKAREGVPDFMTLEAVKGAPAPGSVSYWNLHPYVRRNGPEGNYLGLAPTEFFA
                     DTSELYQLLKKGHHGVEMPQEDWNRLVTWMDMNAPYLGEWPGERNEGLLKRRYDLHGR
                     FSGAERNYVTMKDSLFRRSGGGSISDAGKPITRAGGKSIPVPETRNETLTVDLGNGVN
                     MTFRRIPAGKFRMGNERETPAERPVSTVGIERPFWMGEAEVTLEQYRRFDPEYANGWY
                     DMHYKDQVRPGYDMDADPRFPVIRVPWARAMEFCRWLSGKTGKKVTLPTEAQWEYAAR
                     GGEDTDFFFGERDADFSAYANLGDVTLKELAVSGVDPRPIKNPNRFWDFVPRDEKYND
                     GKLHLAPVKSYRPNAYGLYDMIGNAAEWTRSEYRPYPWKEGDGRNEGLDSRVPRAVRG
                     GSWYDRPRRATSSWRWGYPGWRPVYNVGFRVIIED"
     gene            7305..9167
                     /locus_tag="PK077_03445"
     CDS             7305..9167
                     /locus_tag="PK077_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23382.1"
                     /translation="MKVTKMFRSSAAALGCLLALISCRQGPDIASGPLAPVDVKVVDH
                     LVAVLGHESCVLELVDPASGEKVKSIRLAQPPNGMAVDGATAYVAEGGPRGAVEVVDL
                     KSGELKCSFPAGHTPMSPVVREEKLYVACRFDSRVLEMDAATGTVLNSWNVPREPVAL
                     AVSPDGRKIWAAGHLPAGAADGDFTAAALTLVEDGKAVHFPLSNGTQGVRGMAISPDG
                     RYLAVAHVLSRYQVPTTQLDRGWMNTNAVTVIDTDEPDKPHPVLLDDPDAGAANPWGV
                     SFSGDGGKLFVTHAGTHELSVIDFPALLERMKREDRSNEPVSERLGFLHGLRTRIALP
                     LNGPRSVASDGKNVYVAGYFSDSLAEISLKDACKSRAIPLNAPFRPSREKLGERYFND
                     ASHCFQGWQSCATCHPDGRVDGLNWDLLNDGMGNPKNTRTMFLSHRTSPVMTLGVRAS
                     AEVAVTAGFVHIQFLEPSGELAECVNAYLKNMKEVPSPFLVAHTPSKQQTGGEGCAQC
                     HAPGVERGALSESARRGREVFKTAGCVRCHPHPYFTNKELVATGTATGLDEGKSVLVP
                     SLVEVWRTAPYLHDGRAKTIREAITTCNPGDIRGKTSSLNDRELEDLINYVQSL"
     gene            9185..10309
                     /locus_tag="PK077_03450"
     CDS             9185..10309
                     /locus_tag="PK077_03450"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HRN23383.1"
                     /translation="MTANVPERLEWSGTNGAEECFFCMTAEPGASFEEELRSLMARYR
                     NLGGGEEDEFLLRFHVSDPVRQSAPLRRITGERNSYVSIVGQPPANGARVALEAWHIG
                     GMLGKRRRAEQGGTVVEALRAHYRLFLAGKGEFSSPDSCGQMREEFRWLDRIAALQGG
                     AVPDLIHRTWIYCRDIDNNYRGLVEGRNGCFDRYGLTPESHFIASTGIEGCTELPGRL
                     LHMDSLGIVGLEPGQVRYMEAPDYLSPTHVYRVAFERGVRIVYGDRSHYFLSGTASID
                     AEGNIVHPGDVVGQTARTLENMSVLMERSGGCLSDLKQAVVYLRDWADRETVRNRLMD
                     SPLAAVPHVMLKAPVCRPGWLVEIDGIAVNGAGEPAFAPL"
     gene            10389..10772
                     /locus_tag="PK077_03455"
     CDS             10389..10772
                     /locus_tag="PK077_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23384.1"
                     /translation="MTYRLNTDRLVFLSVLLWVLLPPGVFAWEGNPPSPDNVRWLEQF
                     CSSYVQFLALGGVLCAVIGFVCAVLVWLVHRLDWERSLKISVLFVMSILCLVLYGGSL
                     SFAVFYLPLALINPAVYCFSVSRME"
     gene            complement(10922..>11456)
                     /locus_tag="PK077_03460"
     CDS             complement(10922..>11456)
                     /locus_tag="PK077_03460"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HRN23385.1"
                     /translation="IHQGDFFITGNQNLVIDGITPDTAEQIKSIIGKYNLLPNDSGLR
                     RNSSACTSLPFCPQALTDSERLLPKLVDELEPQLKELGLWDEDISIRMTGCPNGCSRP
                     YLAELAFVGRGPGKYNIWMGGSRRGDRLGFIYQESVPVKEIADVLRPVFARFAAERNQ
                     GEGFGDWSIRALHPQSL"
BASE COUNT         2287 a         3118 c         3666 g         2385 t
ORIGIN      
        1 agggggaccg ctacgtcttc acggaacgtc aggtgaactc catcctggat ctgcgcctgt
       61 accagttgac cgccttggaa aacgacaaga tttccgggga atacgcggaa ctgctggtcc
      121 gcatcaagga ctacctggat attctggcga acgaatcccg cgtgctgggc attgtaaagg
      181 acgaactgaa ggcgattaag gacaagtacg ccacgccgcg cgtcacgcgc atcattccct
      241 tctccgggga tatggccatt gaggatctca tcccgaatga cacgatgatc gtcaccatca
      301 cgcacagcgg ctacatcaag cgcaccaact ccgcggaata ccgcgtgcag gcgcgcgggg
      361 gcaagggcgt gaaggctgcc actaccaggg gagccaaaaa ggatgcggaa gcggacttta
      421 tcgaacacct gttcgccgcc cagaaccacg actacctgat gttctttacg aacaccgggc
      481 gcgtgtacgt ggaccgcgtg tatgagattg acgaggcggc gcgcagcgcg cagggccgca
      541 atatcaagaa cctgctggat ctccagccgg aagaagccat cgctgccatc ctgcgcctgg
      601 aacgccgggt ggatgaaaaa ggcaatgaca ttacctttgc ggaaggcagc ggcaatgttg
      661 ttttcgccac gaaggacggc acggtgaaga aaaccagcct gaacgatttc gccaactatc
      721 gcaaggccgg catcattgcc attaatctgg aggaaggcaa cagtctggtg aacgcggaac
      781 ttaccagcgg ggccgatgaa atcgtgctcg tcacgcatga cggcatgagc atccgcttcc
      841 cggaagaaga tctgcgtacg cagggccgca acaccatcgg cgttcgcggc atccgcccgc
      901 gggcggggga ctacgttgtg gccctggcca ttgtggaccc gcagaaaacc ctgctggtgg
      961 cttctgaaaa cggcctcggc aagcgcactt cctttgacga ataccgcacg cagggccgcg
     1021 gcggcaccgg catcaagacc atgaactgca cggataagac cggcaaggtg gtttccgcca
     1081 cggtcgtgtc cgacgaggac gaactgatgc tgatgaccac ggccggacaa tccgtccgca
     1141 tcaaggtggc gaccgtgcgt gaaacgggcc gcgccaccca gggcgtgaag ctcatgacgc
     1201 tgaatgaggg cgaatccatt caggacattt ccatcgtcat tcccgatgat gaggatgaag
     1261 aagccccggc ggaaacggaa gacgcgggag tcggggaagt gtccggtgaa atggaagctc
     1321 cggcggaaga gtaacatcca ggttttcctg ctccggcgtc atgccggagc agggggcttc
     1381 atgggccgtt tttatggaag accccctttc attggagcaa tgtgccggac gtctggaacg
     1441 gcgtggattt cggagttttg tagtgccggg cctgagggag gctgcggagg tgatgcgcgg
     1501 cctggtgcgg gagttcaggc cgtcgtccgc ttcctatggg gattccatga ccctgaaggc
     1561 tgcgggcatt cttgatgacc tgcactccaa tccggacatt cagttctatg acggctttgt
     1621 tccgggcatg gtgcgggagg agaaatggga aatccgccgc cagggcatga cggcggacct
     1681 gtttttaacc ggcgtcaatg ccgtcagcat ggaagggacc ctgcactggg tggacatggt
     1741 gggcaaccgt gtggctccgg tggcattcgg accccggcgt gtcattttgc tggccggttc
     1801 caacaagctg gtggccacgc cggcagaggc ccgggaacgc atccgccgca ttgcgcccct
     1861 gaacgccagg cggcatgaag ggttcaggac tccctgcatg cacaccggaa tctgcgcgga
     1921 ttgtaattcc ccggaccgcc tgtgcaatat tcacatgtcc attgttaaat gctttcccaa
     1981 gggaagaatt tccgtcattc tgatggaaga acccggagga ctttgaccca tgccttttca
     2041 aaaccggata gaaatcatgg ccccggcggg gtcgtttgaa tctctggcgg ccgccttgca
     2101 gggcggagcg gactccgtgt acttcggagt aggaaagctg aacatgcgtt cccgcgccac
     2161 agtcaatttt tcggaagaag atttgccgga aatcgtcgag cgctgccatg aggcgggcgc
     2221 caaagcttat ctgacactga atatcattgt gtacgatgaa gagctggagg cggtgcatgc
     2281 cctgtgcgac gccgcccgga aagccggtgt ggatgccgtc atcgcttccg acctggcggt
     2341 gatttcttat gcgcgttcca ttgggctgga agtccatatg tccgtgcagg ccaatgtctg
     2401 caacatggcc tccgtcaaat tttacgcgca gtacgcggat gtggtggtgc tggcccggga
     2461 actcaccctg gcgcaaatca ggcatattat tgaatccatc cggaaggagg gcgtgaaagg
     2521 cccctccggg gagctgctgc gggtggaaat tttcgcccat ggggcgttat gcgtggccgt
     2581 gtccggcaaa tgccacatga gcctggcggc ctacaactcc tccgccaacc ggggggcctg
     2641 cttccagaac tgccgccgcg cctaccgcgt gacggatgag gaaacgggca atgaactggt
     2701 gatagacaac aaatacgtga tgtctcccaa ggacctgtgc accattccgg tgctggacca
     2761 gcttctggac gcgggcgttt ccgtgctgaa gctggaaggg cgcggccgtt cctcggatta
     2821 cgtcaggacg gtcacctccg tgtaccggga agccgcgcgg gcatgccagg acggaacctt
     2881 ttccgcggac agggcggaag cgtggatgaa acggctggaa tccgttttca accggggatt
     2941 ctggcaaggc ggctattacc tgggcgtgaa gtggggggaa tggagcggtt ccgccaacag
     3001 ccgcgctgtc ctgttgaaga tccacattgc cagggtagag aacttttata agaagaacgg
     3061 ggtggcggcc ctgttcctgg aagccggcgg cctgtccgcg gggcagacca tcctcataac
     3121 aggccccact acgggagccg tccgcatgga agtggcagcc atgcggaggg agacggcaga
     3181 gggcatggag cccgtaaaag ccgctcaaaa gggagaaacc gtctatctgg cggttcccga
     3241 acaggtgcgc cgccgggaca aggtgtacct gctgcgcccc cggatgctgg aggatgcctg
     3301 aatgtccggt tgtccctttc ttcctctgcc tgccttttcc aaagaccggg aatcgacggc
     3361 aggatgattt taattttgcc gtttcctgcc cggctgggcc ggcggggcgt ccggcttcag
     3421 cagggggagc tggctgagca tgtttctgat cttgatcgtc aggcgcggca tcggctcggc
     3481 tatcgtgagg gctgccactt cataacgggc ggcgatgtcg ttgatcagac ggaccacttc
     3541 atggagtttc attcctcccg gttcctcttc cactcccgcg atgatttcag ccggggtctt
     3601 ttgaaattca cgtttggctt ccggtctaga aatccgaatc aattattcat tcattgtctg
     3661 acgtttgaga gaaaatgccg taaagtagtc ttcctattga atgaaaaaga atttccctgg
     3721 cgtattattt taaaaccgta tgccatgcct gattatattt cctttctgcg tcttttttca
     3781 tgttcctgcg ccttgctgtg tgccggaatg ctccacgctt ccggtttgca gccctggttg
     3841 cgggagctgg ccgtaatccc tcctgcgcct tccgcgaaag aaaaggatgc atggatgcgg
     3901 caggtgtgga cgcggacggc ggagtatgcg gcttcccttt ccgagcctgc ttccgctgac
     3961 gattgcgggg atgcgatggt tttgctggtt cccgtatttc aggcgtggcc ggacgggaag
     4021 gcagccggtg ccgcgctggc ggaaattctt tccgttcccg cggagagtat tggagatgtt
     4081 tcttcctggg atgacctggt taagcatcag gaagccattc aggaacgagt ccgttttctc
     4141 aggctgaaga agctggcggc ctattatgac tctgcggcca tccgccgcgc ggtgaagcga
     4201 aaccgggaca agtacggaga acggtacgcc ggtgcggaag gtttcctggc acggctggat
     4261 gagtgggaga aggagctggg gccgctggac cggtgggtgg agcaggccgg gccgggacag
     4321 gcggccctgc tgcgggagat ggtggatctg cggagaaaag ccctgattga agccctgccg
     4381 gaacagaacg gcctgaagga atgggtgagc gtgcggcgtt tcaatccgtt cgggaaaagt
     4441 tctttcaatc atgaccgtcc ggcgaactgg cagggtattt ccagcatgcc ggggcccggg
     4501 cgcagatacc gcagcgtcat tgtgaaattt aacggggttt cctcttcttc ccctgtggag
     4561 cagctgctgg cggacgaccg ctgggttggg catctggata ttgatttttc cggggagaag
     4621 ctcatgttca ccgggaatgg attcggcaag aaggaaaaca ggccctggga cgtttttgag
     4681 ctggatttga aaaccgggaa aaaggaatcc cttaccgcgc atatgccggc ggatacggac
     4741 agctataatt cctgctacct gccggacggc cggattattt tcgtcaatac ttccggcatg
     4801 cagggggtgc cctgcgtggc cggggtggat tacgtgggca acctccacct ttacgaccgg
     4861 gaaaagaaaa cgacccgccg gcttaccttt gaccaggaca ataactggtt ccctaccatg
     4921 ctgtcggacg gcagggtgat gttcctgcgc tgggagtaca cggaaagcgc ccactatttc
     4981 agccgcgtgc tgatgcacat gaatccggac ggcagcgacc agaaggaata ctatggttcc
     5041 aactcctact ggcccaacag cttgttcaac gcgcggcccc tccccggcag gccaggcatg
     5101 tttgcgggca ttgtcagcgg gcaccatgga gtcaagaggc tgggggaact ggtgcttttt
     5161 gacgtgaaca gggggcgcgc cgccacggag ggtgccgttc agaagattcc ggggtatgga
     5221 aagcccgtgg aaaacgtgac caaagatcag ctggtgcagg gattgaagac tccttatttc
     5281 gcagaacctt atccgttgaa tgacgagtgt ttcctggccg tttcctcccc ctccggcagc
     5341 cagggcgtga ccaatgtggt ctggtgcgac atatacgata acatcgttcc tctcacggcc
     5401 tcctcctact tcgtctatgc agaccctgcg cccctggggc cacgcaaaaa accgcccgtg
     5461 ctgcatgacc gggtgaagac ggaaagcaag acggctacgg tttatatttc cgacgtgtac
     5521 cggggccgcg ccatggccgg agtgcccagg ggggaagcca gggccctgcg cgtgttcatg
     5581 tccgagtaca gcccccgcaa tacgggaagc cactatgcca tgggcatgga gagcaattgg
     5641 gacttgaaag tgctgtacgg caccgtgccc gtgaatcctg acggttccgc catcttcacg
     5701 gctcccgcct gccagccttt gacgcttcag gtgctggacg gggaggggcg tgccctagcg
     5761 ctgatgcgga gctggtttac cgccatgccg ggggaaacgc tcagctgcat cggctgccat
     5821 gaacggcaga actgcgctcc ccccgccagg gccgtcatgg cttcccgcca gaaacccggc
     5881 tctatcgtcc cgtggtacgg ccccgcgcgt accttctcct tcatgaatga agtgcagccc
     5941 gtgctggacc gccattgcat acgctgccac acagcggaag gcaaggcccg tgaaggagtg
     6001 ccggatttca tgacattgga ggccgtcaag ggggctcccg cccccggttc cgtctcttat
     6061 tggaacctgc acccctatgt gcgccgcaat ggcccggagg ggaactatct ggggctggcc
     6121 cccacggagt ttttcgcgga tacgtcggag ctttaccagc tccttaaaaa aggccaccat
     6181 ggcgtggaaa tgccgcagga ggactggaac cgcctggtca cgtggatgga catgaacgcc
     6241 ccctatctgg gcgaatggcc gggagagcgc aatgaagggc tgctgaaacg ccgttatgac
     6301 ctgcacggca gattttcagg agcggaacgt aattacgtga cgatgaagga ttccctgttc
     6361 cggagaagcg gcggcggttc catctctgac gccgggaagc ccattacccg cgccggagga
     6421 aagagcattc ccgttccgga aacgcgtaat gaaacgctga ctgtggattt ggggaacggc
     6481 gtgaacatga cgttccgccg cattcccgca gggaagttca ggatggggaa tgagcgggaa
     6541 acaccggcgg aacgtcctgt ttccacggtg gggattgaaa ggccgttctg gatgggagag
     6601 gcggaagtga cgctggagca ataccgccgc ttcgacccgg agtatgctaa cggatggtat
     6661 gacatgcatt acaaggacca ggtgaggccc ggctacgaca tggatgccga tccgcgtttt
     6721 cccgtcatcc gggtgccttg ggccagggcc atggaatttt gccgctggct ttccgggaaa
     6781 accgggaaaa aagtgacctt gcctacggaa gcgcagtggg aatacgcggc caggggagga
     6841 gaggatacgg atttcttctt cggggagcgg gatgcggatt tttccgcgta tgccaacctg
     6901 ggggacgtga cgctgaagga actggccgtt tccggcgtgg atcccaggcc gattaaaaat
     6961 cccaaccgtt tctgggactt cgtgccgagg gatgagaaat acaatgacgg gaagctccac
     7021 ctggcccccg tcaagagtta caggccgaat gcctacggat tgtacgacat gatcggcaat
     7081 gccgcggaat ggacgcgttc cgaatacagg ccttatccct ggaaggaagg ggacgggcgc
     7141 aatgaaggcc tggactcccg tgttccccgt gcggtgcgcg gcggttcctg gtatgaccgt
     7201 cccaggcggg ccacgtcctc ctggcgctgg ggatatcccg gctggcgtcc ggtgtacaac
     7261 gtggggttcc gggtgatcat agaagactga ttgttgaaat tgccatgaaa gtgacaaaaa
     7321 tgttccgctc ctctgcggct gcccttggct gcctgctggc cctcatttca tgcaggcagg
     7381 gaccggatat tgcttccggc cctctggctc cggtggatgt gaaggtcgtc gatcacctgg
     7441 ttgccgtgct ggggcatgag agctgcgtgc tggaactggt ggaccctgct tccggggaaa
     7501 aggtgaaaag catccggctg gcccagcctc ccaacggcat ggccgtggat ggcgcgacgg
     7561 cctatgtggc ggaaggcggc ccgcgcggag cagtagaagt agtggacctg aaaagcgggg
     7621 aattgaaatg ttcctttccg gccgggcata cgcccatgtc tcccgtggtg cgggaggaaa
     7681 agctgtatgt ggcctgccgg tttgattccc gcgtgctgga aatggacgcc gctaccggaa
     7741 ccgtgctgaa ttcctggaat gtcccccgtg aacccgtagc gctggccgtt tctccggacg
     7801 gcaggaaaat ctgggctgcc ggccatttgc cggccggggc tgcggacggg gatttcacgg
     7861 ctgccgcgct gactctggtg gaagatggaa aagcggtcca tttcccgctt tccaacggga
     7921 cgcagggcgt gcgcggcatg gcgataagcc cggatggacg ctatctggcc gtggcgcatg
     7981 tattgagccg gtaccaggtg cccaccacgc agctggaccg cggctggatg aatacgaatg
     8041 ccgtgacggt tatcgatacg gacgagccgg acaagcctca tcccgttctg ctggatgatc
     8101 cggatgcggg ggcggccaat ccgtggggcg tttctttttc cggagatggc ggaaaactgt
     8161 ttgtgacgca tgcggggacg catgaactct ccgtaattga tttccctgcc ctgctggagc
     8221 gcatgaagcg ggaagaccgg agcaatgagc cggtttccga aaggcttggt ttcctgcacg
     8281 gtctccgcac ccggatcgct ctgcccttga acggcccgcg ctccgtggcc tccgatggga
     8341 aaaacgttta tgtggccggc tacttttctg attctctggc ggaaatctcc ctgaaggatg
     8401 cctgcaaatc cagggcaatt cccctgaatg ccccattccg gccttcccgg gaaaaactgg
     8461 gggaacggta ttttaatgac gcttcccatt gcttccaggg gtggcagagc tgcgccacct
     8521 gccacccgga tggccgggtg gacggcctga actgggatct gctgaatgac ggcatgggca
     8581 acccgaaaaa tacgcgcacc atgttccttt cccaccggac gagcccggtg atgacgctgg
     8641 gcgtccgcgc ctctgcggaa gtggccgtaa cggcgggttt tgtgcacatc cagtttctgg
     8701 aaccttccgg tgaattggcg gaatgcgtga atgcgtacct gaaaaacatg aaggaagttc
     8761 ccagcccgtt cctggtggcg catacccctt ccaaacagca gacagggggg gaggggtgcg
     8821 cccagtgcca tgctcccggc gtggaaaggg gcgccttgtc cgaatccgcc cggaggggca
     8881 gggaagtgtt taaaacagcc gggtgcgtgc ggtgccaccc ccatccttac ttcactaaca
     8941 aggaactggt ggccacgggg acggccacgg ggctggatga aggtaaaagc gttctggtgc
     9001 cctccctggt ggaagtgtgg cggaccgccc cttacctgca cgacggccga gcgaagacga
     9061 tacgggaagc catcacgaca tgcaatccgg gggatatccg cgggaagacg agttctctga
     9121 atgaccgaga actggaggat ttgatcaatt atgtgcagtc cctgtaaagg ggcgggaggg
     9181 catcatgacg gcgaatgtac ctgaacgact ggaatggtcc ggaacaaacg gagcggaaga
     9241 atgcttcttc tgcatgacgg cggagccggg cgcgagtttt gaggaagaac tgcgttccct
     9301 gatggccagg taccgcaatc tggggggagg ggaagaagat gaattcctgc tgcgtttcca
     9361 tgtcagcgac ccggtgcgcc agtcggctcc gctgcggcgg ataacaggag aacggaattc
     9421 ttatgtttcc atagttggac agccccctgc gaacggggcc cgcgtggctc tggaagcctg
     9481 gcacatcggc ggaatgctgg gaaaaaggcg tagggcggag cagggcggaa cggtcgtgga
     9541 agccctccgc gcgcattacc ggctgtttct ggcgggcaag ggggaattct cctccccgga
     9601 cagctgcggc cagatgcggg aggaattccg ctggctggac cggattgccg ccttacaggg
     9661 gggagccgtg ccggatttga ttcaccgcac ttggatttac tgccgggata ttgacaacaa
     9721 ttaccgggga ttggtggaag gccgcaacgg atgctttgac cgttacggac tgactccgga
     9781 gagccatttc atcgccagta ccggcattga aggctgtacg gagctgccgg gccgcctgct
     9841 gcatatggac agtctgggca tcgtggggct ggagcccggg caggtccggt acatggaagc
     9901 gccggactac ctgtccccca cgcatgtgta ccgggtggcc tttgagcgcg gcgtccgcat
     9961 cgtgtacgga gaccgctccc attatttcct ttccggcacc gccagtattg acgcggaggg
    10021 aaacattgtc catccggggg acgtggtggg ccagacggcc cgcaccctgg aaaacatgag
    10081 cgtcctgatg gaaaggagcg ggggctgcct gtccgacctg aagcaggccg tagtgtacct
    10141 gcgcgactgg gcggaccggg aaacggttcg gaaccgactg atggactctc cgttggccgc
    10201 agtgccgcat gtgatgctga aagcccctgt ctgccgcccc ggctggctgg tggaaataga
    10261 cggaatagcc gtcaatggag ccggagaacc tgcctttgcc ccattgtaaa ttctgccgtt
    10321 ccatgaaccg cggcgcgcgg gcgatttccc ttgttatccc gtgcacggaa atggtatcat
    10381 gaaagcgcat gacctatcgc cttaatacgg accgtctggt atttctttcc gtgctgttgt
    10441 gggtgttgtt gccgccgggc gtttttgcct gggaaggtaa tccgccgagt ccggataatg
    10501 tgcggtggct ggagcagttc tgttccagct acgtgcagtt tctggccctg ggcggcgtgt
    10561 tgtgcgcggt gattggtttt gtgtgcgccg tcctggtctg gctggtgcac cgtctggact
    10621 gggagcggag tttgaaaatc agcgtgctgt ttgtcatgtc catcctgtgc ctggttttgt
    10681 atggcggctc cctttccttt gccgtgtttt atcttcccct ggccctgatt aaccccgccg
    10741 tgtattgttt cagcgttagc aggatggaat gagagttcct ggcggcgacg gagggatgtt
    10801 tttcctgggg ggagaagagg ctttataatt ttatttttgg cgtatctttt ttgattttca
    10861 aagatagcag gatttgcccc ggagccgtcc gcggcaaaaa cggctccggg tgtttatgct
    10921 tttataaaga ttgcggatgt aatgcgcgga tgctccagtc accgaatcct tccccctggt
    10981 tgcgttccgc cgcaaaacgt gcaaatactg gtcggagcac gtctgcgatt tcctttacgg
    11041 gtacagattc ctgatagatg aagcccagcc tgtctcctct tctggaaccg cccatccaga
    11101 tgttgtactt gcccggacct ctgccgacga aagccagttc tgccagatat ggtctggagc
    11161 atccgttggg acacccggtc atgcggatgg aaatgtcttc gtcccatagc cccagttctt
    11221 ttaactgcgg ttccagttca tccaccagtt ttggaagcag cctttcggag tctgtaaggg
    11281 cctgcgggca aaatggcagg ctggtacagg cggaggaatt gcgcctgagc cccgaatcgt
    11341 tggggagaag attgtacttc ccaatgatgg atttgatttg ctcggcagta tccggtgtga
    11401 tgccgtcaat gaccagattc tgattgcctg tgatgaaaaa atctccttga tgaatg
//
LOCUS       DAOMSU010000047        11338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_316_length_11338_cov_3.181778, whole genome shotgun sequence.
ACCESSION   DAOMSU010000047 DAOMSU010000000
VERSION     DAOMSU010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11338
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_316_length_11338_cov_3.181778"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="PK077_03465"
     CDS             complement(<1..652)
                     /locus_tag="PK077_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HRN23386.1"
                     /translation="MHLAVRTIDFDYPLPEELIADRPLPDRSASRMMVIHRDTGMIEH
                     RHFCDLPEYVREGDHFILNDTRVVPARYFSNDGSIEIVRAEVLNPLLWKCMVRPGRKM
                     KLGRTVAIGEAVGIVEDIDGEGYRLIRFDREVDEEKYGRLALPHYMNRESDPSDKERY
                     QTVYARHEGAIAAPTAGLHFTPELLASVPHDFLTLHVGVGTFRPVKADRIEDHQMHT"
     gene            747..1796
                     /gene="floA"
                     /locus_tag="PK077_03470"
     CDS             747..1796
                     /gene="floA"
                     /locus_tag="PK077_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961815.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HRN23387.1"
                     /translation="MNNLLSANISNATAWGTILVVVLVIFLVIFFAIIAKFFKTWLRA
                     RLAKAPVSMSNMLGMWLRKVPYPLVVDTRITAAKAGLDISTDELEAHFLAGGDIVDCV
                     LALIAAEKAGIPLSYDRACAIDLAVKGTSKTVLEAVRTSINPRVIDCPNPSSGQTRLT
                     AVARDGIAVAVRARVTVRTNLDLFVGGATEETVVARVGEGIVSAVGSAPSYKDVLEKP
                     EVISRTVSDKGVDAATAFEVLSIDIADVDVAGNVGARLQAEQAEADKQIAQAKAEVRR
                     AAAVATEQEMAAKTQEMRAKVVEAEAQIPMAMAEAFRNGNLGVLDYARYQNVVADTKM
                     RDSIAQPDTPPSSIK"
     gene            1851..2492
                     /locus_tag="PK077_03475"
     CDS             1851..2492
                     /locus_tag="PK077_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23388.1"
                     /translation="MDSLAYMLASGGDDNSLKLWFWLIVGVIFLIKKAIELLKPDEKD
                     EEPSVPDTENHHEKRVREVIAEMRRATPQPQQTQPAAPARAPRPSAPPVELPSPAVAR
                     KPQPIPAPAAAQPEETARQTAQNVLERYVKATEEARQSMRSLSDAEQAALQRLQKREM
                     KEHLPDLLPSSAAVESPSLKNSLHHPKSLKAAVLYQEILGKPRALRSASFSGE"
     gene            2598..3380
                     /locus_tag="PK077_03480"
     CDS             2598..3380
                     /locus_tag="PK077_03480"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HRN23389.1"
                     /translation="MTDTPLQIAGRQFTSRLMVGTGKFPSNESMKEALEASGAQIVTV
                     ALRRADLTGSHDPAANILDFIDPEKYLLLPNTSGATTAEEAVRLARLAAAAGLPNWIK
                     LEIHPDAQYLMPDPIETLKAAEILVREGFTVLPYINADPVLAKRLQEAGAAAVMPLGA
                     PIGTNKGVLTRELINIIIEQAIVPVVVDAGLGAPSHAAEALEMGADAVLVNTAIAASG
                     SPSAMAEAFALACRAGRMAYLSSLPAVSSQATATSPLTSFLH"
     gene            complement(3422..4726)
                     /locus_tag="PK077_03485"
     CDS             complement(3422..4726)
                     /locus_tag="PK077_03485"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRN23390.1"
                     /translation="MYGKAPKEYLSLWSVIALGIGSMVGAGIFALMGQATLMAGKNVY
                     WSFILGGVAALLSGYTYAKLGSRYPGSGGIMDYFNEAFSHKTLSGALTLIYLGTLVIT
                     VALVAKSFGAYASRLFLPDRAVTIYSTALFASVAILLLGALNMGGARAVGKSEVIMVA
                     FKLLILTVLMLASFGSSVPVGADPIPVKGLDGLLGSVGLTFFAFAGYGMMANTAGNLK
                     NPSRTLPRAIFMAIGLVLALYLILSYVVLSNVPATSLESHTETAVAQAAYPVLGMWGF
                     MAVSVAALIATASAINATMFSMLDISRALARNGQLGAIFKQPFWGHGTEGFFYLLLGI
                     LVMTNAFNLVAIANLAGACFLISYLAVFVAHWRLRKETQGNVLLIILGFLLMLFILGA
                     FFYQMFFSQPYLIPLIVLFVAACFILEFLIRRKIAKNVPGVS"
     gene            4880..5545
                     /locus_tag="PK077_03490"
     CDS             4880..5545
                     /locus_tag="PK077_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRN23391.1"
                     /translation="MIKTAIFDFDGTLADTIPLCREAFRRAIRKLDGRILTDEEIERQ
                     FGPDDLGVIQKLIPGKPELHERGRELFIHYYRKLHADLAPAPFPGTAELLSTLRNLGI
                     RLAMVTGKRLESAEISLQFFHLSEFFPILETGSPEGGVKPDRIKRALSRLNAVASEAI
                     YIGDAPTDVDACRSVPIRILSAGWAAEADINGLREKKPDFLLTRFEDLERFFREHHEN
                     EGL"
     gene            5605..6717
                     /locus_tag="PK077_03495"
     CDS             5605..6717
                     /locus_tag="PK077_03495"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRN23392.1"
                     /translation="MTFQKESGKEGLKILLMLASVIIITAGLQAGKPVLLPIVLSGFL
                     AIVSYPLTTFFKSRLCFPHWLAVTFTVIMDFGILVGLGYLAQYLGQDLAKTVTVKYQP
                     LMMEKIHELRAFLIEQDWNNLADQMLQELPDLLNGQRIVAFSTGVMGQLASMLTFTTL
                     ILILMTFFLGEAPRFRANINKLGHNSDTGIRKFSKALAGVQKYLIIKTFISAVTGLLA
                     FLLCYYMNVDFPLLWGIVAFALNFIPTFGSIIAAIPPTLLAMLLISPTAGIIVAGGYL
                     VINTALGNCLEPMLLGRQFGIVTSMVLLSVIFWGWVWGPIGMLLAVPITMLIKLGLES
                     SKDLAWIAQLIDNPPTPRFPLPPLHSGKTNESTTKE"
     gene            6721..8010
                     /gene="lysA"
                     /locus_tag="PK077_03500"
     CDS             6721..8010
                     /gene="lysA"
                     /locus_tag="PK077_03500"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HRN23393.1"
                     /translation="MHSFAYKNGTLYCENVNLQELADKESTPLYVYSKQTILNHFHRL
                     REALAPLNAEVAYAVKACSNIAILNLMARNGAGFDIVSGGELFRVLKAGGDPSKCTYA
                     GVGKTEQEIRYALAQGIYCFNVESEAELRAINAIAASMGVKAPVAVRVNPNVEAGTHK
                     YITTGKAENKFGVDFERIESLYEMAARELLNLHLKGLQMHIGSQLTQAKPFLEAVRKV
                     APLAASLKEKHGIEFFSIGGGIGIVYQGTLDSGVQEWWNEDCAQLTLSTYAQAVVPTL
                     QPLGLHIIVEPGRLIVGNAGALITRCLYEKNGKAKTFKIVDAGMNDLIRPALYQGYHE
                     IIPVREYPSESCVTADVVGPICESGDFLAQNRDMPDVHQGELLAVLSAGAYGFSMSSN
                     YNSRPMAEEVLVDGDQWNVIRSRQSWEDLIRGESIPE"
     gene            8305..9336
                     /gene="fbaA"
                     /locus_tag="PK077_03505"
     CDS             8305..9336
                     /gene="fbaA"
                     /locus_tag="PK077_03505"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HRN23394.1"
                     /translation="MPIATPEQYITMLETAKKEGYAYPAVNVTTIEVINGALRAFSEA
                     KSDGIIQVSIGGGKFASGSSVGNSALGAIVLAEATRRLAEKHNVLVALHTDHCQPEHV
                     DTFLRPLIAESRRRVERGEAPLFNSHMLDASTLPMAENLKLSRELLKECAELGIVLEI
                     EIGVVGGEEDGVDNSGHPADKLYTSPEDMLMTYEALAPLGKFMLAATFGNVHGVYKPG
                     HVKLKPSILRDGQEAVVAKHGEAARMPLVFHGGSGSELSDIREAVSYGVVKMNIDTDT
                     QYAFTRPIVSHMCEHINGVLKIDGEVGNKKDYDPRSYLAKGEKGVAARLQEAADNLMS
                     AGKTLFGKI"
     gene            complement(9722..>11338)
                     /locus_tag="PK077_03510"
     CDS             complement(9722..>11338)
                     /locus_tag="PK077_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRN23395.1"
                     /translation="IVDTPGHADFGGEVERVMKMVDGVLLVVDAFEGPQAQTRFVLRK
                     ALQEGLMPIVVINKIDRPHADPAAVHDQVLELFLELGATDEQFEAPFVYGSARDGYFM
                     NSMEGEPPVDCTPLLFKIVEHIPAPKDADPEGEFKMLVSNIDWDDYVGRVAIGRITSG
                     TVKKGDTVWLHQSDGSCISGKVTRMFEYSGLSTTDSAIGVAGNIVGVAGFENVNIGET
                     LGGSADTEPLPFVAIDPPTISMEFSINNGPFGGRDGKNVTSRVIRDRLMREMRTNISI
                     QVEDTDKAGVFSVSARGAMQIAVLVETMRRENYELCVSRPTVIMKRDENDRLTEPYET
                     MYVEVPDEHANGVMKLLHARKGQMEDMVCREGTGHTFIQAYIPTRGIIGFEFELVNLT
                     SGHGIFSHLFRDYGPYAGDITTRTTGTLVSMETGVSTPFALVALEERGRLFVGPQEDI
                     YEGQIVGENPRQMDMPVNPCKEKHLNNIRSATKGDGIQLSPPVIFSLERAIEYIEPDE
                     LVEATPHFIRLRKRILNANDRVRESRKAGN"
BASE COUNT         2685 a         3442 c         2896 g         2315 t
ORIGIN      
        1 ccgtatgcat ctgatggtct tctatcctgt ccgccttgac gggacggaat gttcccacgc
       61 ccacgtgcag ggtcaaaaaa tcatgaggta cggacgccag cagctccggc gtgaaatgca
      121 gcccggccgt aggggcggca atggcccctt catgcctggc gtaaacggtc tgataacgct
      181 ccttgtcgga agggtcgctt tcccggttca tgtaatgggg aagagccagg cggccgtatt
      241 tttcctcatc cacttcccgg tcaaaccgga tgaggcggta tccctcccca tcaatgtctt
      301 ccacgatgcc taccgcctcc ccgatggcaa ccgtacgccc aagcttcatt ttgcgtccgg
      361 ggcgcaccat gcacttccac aacaggggat taagaacctc cgcgcgtact atttcaatgg
      421 aaccgtcgtt ggaaaaatat cttgccggaa ccaccctggt atcattcagg ataaaatgat
      481 ccccttcccg cacatattcc ggcaaatcgc aaaaatgcct gtgctcaatc atgcccgtat
      541 cccggtgaat gaccatcatc cgggacgcgg agcggtccgg aaggggacgg tccgctatca
      601 gttcttccgg cagcggataa tcaaaatcaa tggtgcgcac ggccaagtgc attgccacga
      661 atcctccgaa aggtcaatat ctttaaaccg caggtaacac aattatatct ttaccctgct
      721 tcggttcata tggtatggaa ggatacatga acaacctgct gtcagcaaac atttccaacg
      781 caaccgcctg ggggaccatt ctggtcgtcg tcctcgtcat ctttctggtc atctttttcg
      841 ccatcatcgc caaattcttc aagacatggc tgcgcgcccg tctggcgaaa gcgcccgtct
      901 ccatgagcaa catgctgggg atgtggctcc ggaaagtgcc ctatccccta gtggtagaca
      961 cccgcattac ggccgccaag gcgggcctgg acatcagcac ggatgagctg gaggcccact
     1021 tcctggccgg cggcgacatc gtggactgcg tgcttgccct gattgcggca gaaaaagccg
     1081 gcattccgct gagctacgac cgcgcctgcg ccattgactt ggccgtgaaa ggcacttcca
     1141 agaccgtgct ggaagccgtg cgcacctcca tcaatccccg agtcatcgac tgcccgaacc
     1201 ccagttccgg ccaaacgcgc ctgacggcgg tggcccgtga cggcattgca gtggcggtgc
     1261 gcgcccgcgt aacggtgcgc accaacttgg atctcttcgt agggggcgcc acggaagaaa
     1321 cggtagtcgc ccgcgtcggg gaaggcatcg tttccgccgt aggttccgcc ccctcctaca
     1381 aggatgttct ggaaaagccg gaagtgatct cccgcaccgt aagcgacaag ggggtagatg
     1441 ccgccacggc gtttgaagtg ctttccattg acattgcgga tgtggatgta gccggcaacg
     1501 tgggcgcccg cctccaggcc gagcaggcgg aagcggacaa acaaatcgcc caggccaaag
     1561 cggaagtccg ccgcgccgcc gccgtagcca cggaacagga aatggctgca aaaacgcagg
     1621 aaatgcgcgc caaggtggtg gaagcggaag cacagattcc catggccatg gcggaagctt
     1681 tccgcaacgg caatctggga gtgctggact acgcccgtta ccaaaacgtg gtggctgaca
     1741 ccaaaatgag agattccatc gctcaaccgg atacgcctcc ctcttccatc aaataaataa
     1801 attccctggt ttccggaaag gcctgctttt tccggaaacc tttatccttt atggattcac
     1861 tggcatacat gctcgcctcc ggaggcgatg acaatagcct gaaactctgg ttctggctga
     1921 ttgtcggggt gattttcctc atcaaaaaag caatcgaatt gttgaaaccg gatgaaaaag
     1981 atgaggaacc gtccgttccg gacacggaga accatcatga aaaacgcgta agggaagtca
     2041 ttgcggaaat gcgccgggca accccgcagc cacagcaaac ccaacctgcc gcaccggccc
     2101 gcgcaccgcg gccctcagcc cctcctgtag agcttccctc ccccgccgtg gcccgcaaac
     2161 cgcagccgat cccggcgccg gcggctgcac aacccgagga aacagcccgg cagacggcgc
     2221 aaaatgtgct ggaacgctac gtaaaagcta cggaagaagc ccggcaatcc atgcgctctc
     2281 tttccgatgc ggaacaggcg gctctgcaac gtttgcaaaa gagggagatg aaggaacatc
     2341 tgcccgacct tcttccgtca tcagccgcag tggaatctcc ttccctgaaa aattcccttc
     2401 accaccccaa atccctgaaa gcggctgttc tttatcagga aatccttgga aaacccagag
     2461 cattacgaag cgcatccttc agcggtgaat aaagaatggc ccttcccggg ccatgttccg
     2521 ttcagccgtc atgctgcccc cctcttattc attctccctt gcctgaaaag gtaagcggag
     2581 gtaccctcca tcccgcaatg acagacacac ctttgcagat agccggacgc cagttcacct
     2641 cccgcctgat ggtgggaacg ggaaaatttc cttccaatga gagcatgaaa gaagctctgg
     2701 aagcctccgg cgcccaaatt gtgacggtgg ctctgcgccg ggcggatctg acgggttccc
     2761 acgatcccgc cgccaatatt ctggatttca ttgatccgga aaaatacctc cttctcccca
     2821 acaccagcgg agcgactacg gcagaagaag ccgtacgcct ggcgcgcctg gcggcagcag
     2881 ccgggctgcc caactggatc aagctggaga ttcacccgga cgcccagtac ctgatgccgg
     2941 accccattga aacgctgaaa gcggcggaaa tcctggtcag ggaaggcttc accgtgcttc
     3001 cctacattaa cgcggatccc gtgctcgcca aacgccttca ggaagccgga gcggcggccg
     3061 tcatgcctct tggcgccccc atcggcacca ataagggagt gctgacccgg gagttgatta
     3121 acatcatcat tgaacaggct attgtccccg tggtggtgga cgcggggctg ggagcaccca
     3181 gccacgcggc ggaggctctg gaaatgggag cggatgccgt gctcgtcaat acggctatcg
     3241 ccgcctccgg ctctccctcc gccatggcgg aggcgttcgc cctggcatgc cgtgccggac
     3301 gcatggctta tctgtccagc cttcccgccg tttcctccca agctacagcc accagccccc
     3361 tgacttcctt cctgcattaa aaggccggga aaaagccccg gttttctccc gcacccatgg
     3421 gtcaagaaac tccgggaaca tttttcgcaa tcttcctgcg tatcaggaat tccaggatga
     3481 agcaggcggc tacaaacagc acaatcaacg gaatcagata aggctggctg aagaacatct
     3541 ggtaaaagaa cgcccccaga ataaacagca tcagaagaaa gcccaggatg ataagcagca
     3601 catttccctg agtctctttc cggagccgcc aatgggcgac gaacaccgcc aggtagctga
     3661 tcagaaaaca ggcccccgcc agattggcga tggctaccag gttaaaggca ttcgtcatca
     3721 ccagaatccc cagcaaaaga tagaaaaaac cctccgttcc gtggccccag aaaggctgtt
     3781 taaaaatagc tcccagctgg ccgttgcgcg ccagagcccg ggaaatgtcc agcatgctga
     3841 acatggtggc attaatggct gatgccgtgg caatgagagc cgctacggat acggccatga
     3901 agccccacat gcccaaaacc ggataggcgg cctgcgctac ggccgtttcc gtatggcttt
     3961 ccagggaagt agcgggaacg ttggacagta ctacgtagga gagtatcagg tacaaggcca
     4021 gcacaaggcc aatagccata aaaatggctc ttggcagcgt cctggaagga tttttcagat
     4081 tgcccgccgt attggccatc attccgtagc cggcaaatgc gaaaaatgtc aatcccacgc
     4141 tgcccagcaa accgtccagc cctttcaccg gaatgggatc cgcgcccaca ggcacggaag
     4201 aaccgaagct ggcaagcatt aaaactgtca aaatcaataa tttgaatgcc accatgatta
     4261 cttccgactt tccaacggcc ctggctcccc ccatattcaa tgcccccagc agcaaaatag
     4321 ccacgctggc aaaaagggcc gtggaataaa tggtgactgc cctgtccggt aaaaacaggc
     4381 gggacgcata ggccccgaag gatttggcca ccagggccac ggtaatgacc agcgttccca
     4441 ggtaaatcag cgtcagcgcg ccggagaggg tcttatggga aaaagcctcg ttgaaataat
     4501 ccataattcc tccggaaccg ggataacggg aacccagttt ggcgtaggtg tacccggaaa
     4561 gcagggcagc aactcctccc agaataaagg accaatatac gttttttccc gccatcaggg
     4621 tagcctgccc cataagggcg aaaattcccg ctcccaccat ggaccctatt cctaatgcaa
     4681 tgacgctcca cagggacaga tattcttttg gggccttccc gtacataagg gatttagaga
     4741 tactacagga atgaaacgtt gacctttccg cacgggatga cacgtcctgc ttattcaccc
     4801 cctcctgcag ctccatcatg actattccgg cacggctttc cccttgaacc gtccgttgtc
     4861 ggcatgtagg gtacggctga tgatcaaaac cgccattttt gattttgacg gaactcttgc
     4921 cgacaccata cccctgtgcc gggaggcttt tcgccgcgct atccggaagc tggacggcag
     4981 aatattgacg gatgaagaaa tcgaacgcca attcggcccg gacgatctgg gggttatcca
     5041 gaaattgatt cccgggaaac cggaactgca tgaacgcggc agggaactat ttatccacta
     5101 ctaccgcaag ctgcacgcag acctggctcc cgcccccttt cccggaacag ccgaattgct
     5161 gagcactctc cgcaacctcg gcattcggct ggccatggtt acaggcaaac gcctggaaag
     5221 cgcagagata tctctccagt tttttcatct gtccgaattt tttcctattc tggaaacagg
     5281 ctccccggag ggaggcgtaa aaccagaccg catcaaacgg gctctgtccc gtctgaatgc
     5341 cgtcgcctca gaagccatct atattggaga cgcccccaca gacgtggacg catgccgctc
     5401 tgtccctatc cgcattctct ccgcaggatg ggccgcggag gcggacatca acggcctcag
     5461 ggaaaaaaag ccggactttc tcctgacccg ttttgaagac ctggaacgtt tcttccggga
     5521 acatcatgaa aatgaaggcc tttaagattg caatcctgca aaaaacaggc tatcattacc
     5581 caccccgtta atatcaatcc ttccatgact tttcagaaag aatccggaaa agagggcctg
     5641 aaaattctgc tcatgctcgc cagcgtcatt atcatcacgg ccgggctgca ggcgggaaaa
     5701 ccggtgctcc tgcccatcgt tttatccggc tttctggcaa tcgtcagtta tccgctgacg
     5761 acttttttca agagccgtct ttgcttcccg cactggctgg cagtgacttt cacggtcatc
     5821 atggactttg gcattctggt gggcctgggc tatctggccc aatacctggg gcaggatctg
     5881 gccaaaacag tcacggtcaa ataccagccc cttatgatgg aaaaaatcca tgaactccgc
     5941 gctttcctga ttgaacagga ttggaacaac ctggctgatc agatgcttca ggaacttccg
     6001 gacctgctca acggccagcg catcgtggcg ttttccacag gggtgatggg gcagttagct
     6061 tccatgctga ccttcaccac cctgattctg atcctgatga ctttcttcct gggggaagcc
     6121 ccccgcttcc gggcgaacat caataaactg gggcataaca gcgacacagg catccgcaaa
     6181 ttctccaagg ccctggccgg agttcagaaa tatctcatta ttaaaacctt catcagcgca
     6241 gttacagggc ttctggcttt cctgctttgc tattacatga acgtggactt tccgctgttg
     6301 tggggcatcg tggctttcgc cctcaacttc attcccacct tcggctccat catcgcggct
     6361 attcccccca cgcttctcgc catgcttctg atcagcccta ctgcgggcat cattgttgcc
     6421 ggcggctacc tggtgatcaa cacagccttg ggaaactgcc tggaacccat gctgttggga
     6481 cgacaattcg gcattgtgac cagtatggtt ctgctctccg tcatcttctg gggctgggta
     6541 tggggcccca tcggcatgct gctggccgta cccattacca tgttgattaa actcgggctg
     6601 gaaagctcca aggatctcgc ctggatcgcc cagctcattg acaaccctcc cactcccaga
     6661 ttccctctcc cccctctcca ttccgggaaa accaacgaaa gcacaaccaa ggaataatcc
     6721 atgcattcat tcgcttacaa aaacggcacg ctctactgtg aaaacgtcaa ccttcaggaa
     6781 ctggcggaca aggaaagcac acccctgtac gtttatagca aacaaaccat tttaaaccac
     6841 tttcaccgcc tcagggaagc tctggcaccg cttaacgcgg aagtggccta cgccgtcaag
     6901 gcctgctcca acatcgccat cctgaacctc atggcccgca acggggcggg attcgacatc
     6961 gtctccggcg gagaactctt ccgcgtcctc aaagccgggg gagatccgtc caaatgcact
     7021 tatgccggcg taggaaaaac cgagcaggaa atccgttatg ccctggcgca gggcatttat
     7081 tgcttcaatg tggaatccga agcggaactg cgggccatta acgccattgc cgcctccatg
     7141 ggagtcaagg ctccggtggc cgtgcgcgtc aatcccaacg tagaggcggg aacgcacaaa
     7201 tacattacta ccggcaaggc tgaaaataaa ttcggcgtgg acttcgaacg cattgaatct
     7261 ctttatgaga tggcggcccg cgaacttctg aaccttcatc tgaaagggtt gcaaatgcat
     7321 atcggctccc agcttaccca ggcgaaaccc ttcctggaag ccgtcaggaa agtcgccccc
     7381 ctggctgctt ccctgaaaga aaaacacggc attgaatttt tctccatcgg aggaggaatt
     7441 ggcatcgttt accagggcac gctggattcc ggcgttcagg aatggtggaa tgaggactgc
     7501 gcccagctca cgctgagcac ttacgcccag gccgtcgtcc ccaccctgca acctctggga
     7561 ttacacatca ttgtggaacc gggccgtctt atcgtaggaa atgcgggagc actcatcacg
     7621 cgttgcctgt atgaaaaaaa cgggaaagcc aaaaccttta aaattgtgga tgcagggatg
     7681 aacgacctca tccgccccgc cctctaccag ggctatcatg aaattatccc ggtcagagaa
     7741 tacccctccg aatcctgcgt cacagcagat gtcgtgggtc ccatctgtga atccggagac
     7801 ttcctcgccc aaaacaggga catgccggac gtgcaccagg gagaactcct ggccgtactg
     7861 tccgccggag cctatggttt ttccatgtct tccaactaca attcacggcc tatggcggaa
     7921 gaagtcctgg tggacgggga ccaatggaac gtcattcgca gccgtcaaag ctgggaagac
     7981 ctcatccggg gagaatccat tccggaataa catccccatc ccgcccatct ttagaatgcc
     8041 cttccagcgg taaaacgaag gaggggcatt tcctttcaac cgtactgaat ggaaaacaag
     8101 gtagaatttt caagtccgcc aagccacgcg gagcttcttt ccggagacgc cgcacgctac
     8161 tgccgtacgg actccggtgg accagtccgg ccgcaagtca caatgattcg aacatgaaca
     8221 tgtcccgcaa gagcgctgca accccggcgt gcgcctgagt tcagccttgc cctcctgaaa
     8281 aaaaacacct atcatatccc cgttatgcca atagccacac cagaacaata cattaccatg
     8341 cttgaaacgg ccaaaaagga aggctacgcc tatccggccg ttaacgtgac cacgatcgaa
     8401 gtcatcaacg gcgctctccg cgctttttcc gaagccaaaa gcgacggtat catccaggtt
     8461 tccatcggtg gcggcaaatt cgcctccggc agctccgtag gcaattccgc ccttggcgcc
     8521 attgttctgg cggaagcaac gcgccgtctg gctgaaaagc acaacgttct cgtagccctt
     8581 cacacggacc actgccagcc cgaacatgtg gatactttcc tgcgtcctct gattgccgaa
     8641 tcccgccgcc gcgtggaacg cggtgaagcc cccctcttca attcccacat gctggatgcc
     8701 tccacgcttc ccatggcgga aaacctcaag ctttcccggg aactgctgaa ggaatgcgcc
     8761 gaactgggca tcgttcttga aatcgaaatc ggcgttgtgg gcggagaaga agacggagtg
     8821 gacaactccg gccatcccgc cgacaagctt tacacttctc cggaagatat gctgatgacg
     8881 tatgaagccc tcgcgcccct cggcaaattc atgctggccg ccactttcgg caacgtgcac
     8941 ggcgtgtaca agcccggcca cgtcaaactg aaacccagca tcctgcggga cggtcaggaa
     9001 gccgtggtag ccaaacacgg agaagcggct cgcatgcccc tcgtcttcca tggcggttcc
     9061 ggttccgaac tttccgatat ccgcgaagcc gtttcctacg gcgtcgtcaa gatgaacatc
     9121 gacaccgaca cgcaatacgc cttcacccgc ccgatcgtct cccacatgtg cgaacacatc
     9181 aacggagtgc tcaagattga tggcgaagtg ggcaacaaga aggattacga tccccgttcc
     9241 tatcttgcca agggtgaaaa gggagtcgcc gcccgccttc aggaagccgc cgacaacctt
     9301 atgtccgccg gcaagaccct ctttggcaaa atctaaaaag agttccctca acgagtttaa
     9361 tgccatgccc ggtttcttaa aagaaaccgg gcattttttc tggaaaaacc cgccaacagg
     9421 gtaaggcggg aaacgctttc accgagtatt tatccctctg agacgacggg aaaacgaacc
     9481 gttccccgtc ttcttctttt ctgtcccctg ggcttctccg attacgggac cattaacccg
     9541 cattcttccg caaggaaacc gctgcggaca aataaaacga tagccgcaca ggaaaaacca
     9601 gaattattca taacagaaca aggccattcc tccgctcagc ggaccgcagg gaacccggca
     9661 gcgcggccct accccggcgc cgctttgaac acaggagcct ttccccagcg ctccaggaac
     9721 cttaatttcc tgctttgcgg gattccctta cgcggtcatt ggcgttcagg atgcgcttgc
     9781 gcagacggat aaaatgaggc gtagcctcca ccagttcatc aggttcaatg tactcaatgg
     9841 cacgttccag agaaaaaatg acgggagggg aaagctggat gccgtctccc ttcgtggctg
     9901 agcggatatt gttcaaatgc ttttccttgc acgggttgac cggcatatcc atctgccgcg
     9961 ggttttcgcc tacaatctgg ccttcgtaaa tatcttcctg cggccccaca aacagacgcc
    10021 cgcgttcttc cagcgctacc agcgcgaacg gagtggaaac gcccgtctcc atggaaacca
    10081 gggtgccggt ggtacgcgtg gtgatgtccc cggcataggg gccgtaatcc cggaacaggt
    10141 gggagaaaat accgtggccg gacgtcagat tgaccagctc aaattcaaaa ccgataatac
    10201 cgcgggtggg aatataagcc tgaataaaag tatggcccgt gccttccctg cataccatgt
    10261 cttccatctg tcccttgcgg gcgtgaagaa gcttcatgac gccgttggca tgttcgtccg
    10321 gaacttctac gtacatcgtt tcatacggtt ccgtaaggcg gtcgttttca tccctcttca
    10381 tgatcacggt gggacgggaa acgcacagtt cataattttc acggcgcata gtctccacca
    10441 gcacggcgat ctgcatggcg ccgcgggcgg aaacggagaa aacgccggcc ttgtccgtat
    10501 cctccacctg gatggaaata ttcgtccgca tttccctcat caggcggtcg cgtatcacgc
    10561 gggaagtcac attcttgccg tcgcgccctc cgaaaggccc gttgttgatg gaaaattcca
    10621 tggaaatggt aggaggatca atcgccacga atggaagggg ctccgtatcc gcggaaccgc
    10681 ccaaggtttc cccgatattg acgttttcaa accccgccac ccctacgatg ttgcctgcca
    10741 cgccgatggc cgaatccgta gtgctcaggc cggaatattc aaacatgcgg gtcacttttc
    10801 cggaaatgca ggagccgtcg ctctgatgga gccacacggt gtcccctttc ttcacagtgc
    10861 cggaggtaat gcggccgata gccacacggc ccacataatc gtcccagtca atattggaaa
    10921 ccagcatctt gaattcaccc tccggatcgg catccttcgg agcggggata tgttccacaa
    10981 tcttgaataa aagaggagtg cagtccacag gaggttcccc ctccatggaa ttcatgaagt
    11041 aaccgtcgcg ggcggaaccg tacacgaaag gagcctcaaa ttgttcatcc gtggcgccca
    11101 gctccagaaa aagctccagc acctggtcat gcacggcggc gggatcggca tgcggacggt
    11161 cgatcttgtt aatgacgaca atgggcatca gaccttcctg aagggccttg cggagcacaa
    11221 aacgtgtctg ggcctgcggc ccttcaaaag cgtccaccac cagaagcacg ccatccacca
    11281 ttttcatcac gcgctccact tccccgccga aatccgcgtg tccgggagta tccacaat
//
LOCUS       DAOMSU010000048        11210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_330_length_11210_cov_2.789153, whole genome shotgun sequence.
ACCESSION   DAOMSU010000048 DAOMSU010000000
VERSION     DAOMSU010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_330_length_11210_cov_2.789153"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4439)
                     /locus_tag="PK077_03515"
     CDS             complement(<1..4439)
                     /locus_tag="PK077_03515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRN23396.1"
                     /translation="MKSNFSICNSVRGNHHLGFTVTPVLQKREGESSVSFSYNHAAPS
                     YPGDVMPETSKQIQVGEGGGDTRLYVLTATGTVDDQATISVAGQSYTSPSGAGVRING
                     SFSDLKAGFYTVSVSHINIDYPPRGNVSFLNCTVGPASAISITPDENDPPQECDCDCP
                     PCSCNSDGETTPGGVPSSSSAPVSGRSSLRHFFSNLTTTSGGSGVMRQSSVNGMAWSA
                     QFGAFRGISHMPAGRLEISAHRSFSPDLFSPAGLALRHPLATFLALPEGGMAPNTLVA
                     LLDGASYTNYMLDGTGSAFFPVGASGITTTILAPVPAMSREAEACNLEQAAFIRASYA
                     GGGSVFYSLSTGQCEGYISAGGYLMTASEAENYLAIVRGEHGVIRQIWNTWDGLADVL
                     PLEGGGYAISIYPPAQVMEPEEEGALFTVEGTPAKIFTVTGDEALQTLVISERDNTLP
                     ATVPDFVTKWTWSQEAWSMAEGTGEDAVETRRERLQPEEDSQENRYRVLTRLMKNNVV
                     ASCTLEEYETTITGEHLLSRTEGYGAEGALTTVYSYDDSGRLISSASPNAGEHKTVYD
                     AAGRPVLENSPWGANGLRTVSTKYRDSAAGYTSEPAEIKIQLSTVSAVNTLSLEVYTY
                     TEENHVKRVEKRETAAGSSVTQLSVTETWLAGAPNAFAAGRLKMEQAVNGVQTHYTYA
                     PTTDHGALYSVTAETRINGEPVPGQSTRRVSYITAEGNTAREENLVLLPTGEWRQTGG
                     ASYSYDLLNRRVETVRDNGRTSSRALTCTGEPLWEVDEDGVRTDYAYNTARQLIETTR
                     SEINDGTDVVTPETITAYERDAAGQIISATTHIGPMRTTEETAYDLLGRVTRKTDVLG
                     RVTTTSYSEDGLTVTTTTPSGATLVNTYNADGSIAHESGTGQRELYHIYDFIRGLRHT
                     TRLADNTTILKQEIRDGFGQTITLTSPTTLENTYLYTRSVYNALGQLTRRTEGTQAPV
                     EYEYDSMGNPARQTVVLDSASPADAAKNRIQTFQTAWEEREDGVYQNVAAARNNTAGN
                     MLSSTVKRLVTESAVLQNRQITIDERGHESIRWTEYGQGAVRVEKSSVPTSDVTASAT
                     VVDGFTISETDNRGIVTAFTRRYTETGLVRTVTDGRGNASTTHTDIAGRPIAVTDAAG
                     NTTTTTYCATSDNPSAVTNALGNTANYAYDIRNRKTAEYGTAIQPALFAWDDADRLIS
                     LTTFRIDEETIISDPSDLSGGDTTVWTYHDATGLELGKTYANGRGTVRTYDANNRLAT
                     ETDARGIVTTYTWNAAQGLLTRIGFSDGTPEQAFSYNILGQLTQVTDASGIRTFSYNE
                     YSDPVQDSVTVNGAVFPLVETLDGYGRSIGYILKRGASGNAQIASWGYAPDGRINSAG
                     FMHSGQNRIFEYNYLAGSNLLETLTIPNGMTLEQSYEAKRDLLADMDYNRGTTLVSRR
                     AYAYDALGRPTVRTLLRQGTTRRDVFSYNGRSELAAATLGTDA"
     gene            4776..6401
                     /locus_tag="PK077_03520"
     CDS             4776..6401
                     /locus_tag="PK077_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN23397.1"
                     /translation="MKNLLSRYISLSLALRYLNPLRTFFSIITLICLLGVSLGVMVLI
                     VVLSVMGGLQKEIQGNLFAHSPHVQVCYRNDFGVREVIPDWLDLGEKLRHVPGVQSAY
                     ALIEDYALADVQGRQRPCFFRAIDTENAAQLEDLKRLVVAGDAELDMGEKAVVSSIVA
                     ENMGLKVGDVVRVYTTRNFQEISHAYQQTELPMLAEKNARELKDLKEWGKSLKTEGER
                     ETADRASVDRAFSLLNGLLSVPRRDAERSSLMDLLAVLNDGEIADNGRVAFPQGTRKE
                     WETVLDGFKAGARNEADMECFRKIKELVMPKDLEVIGIYRASQHTPSPDLFIPLVIGQ
                     ELLGYEDDVVQAVALRVEDPYHVETMLAPVMQALEKEKPSSAWTLETWHDRFNAWFEL
                     MQKERMMMSFVLSFISLISAFCIMAVMFTVSIQRKKEIAVMKALGATPFQVVRVFLWQ
                     GVIIGFVGALLGVGLGLLVLEYRMQIQGFLAGIGFDPFPVAFHGTANIPVVIDWAELA
                     WQAVKAFVMVVVASIIPALITARQDPARSLRSM"
     gene            6414..7280
                     /locus_tag="PK077_03525"
     CDS             6414..7280
                     /locus_tag="PK077_03525"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HRN23398.1"
                     /translation="MDIYWIQGHEQKGPLPEVEVISMLEAGLIPENARAWHAGCPEWV
                     CIRDLPALKGAGAVSRGEGERRNREEEGLENAGEEQAPALAADADCPQEEGGTASEEG
                     APLVVPYAYVRFLGRMADIMMHMTLYLSVLRVSGLAFNPGFLPGSYEALLYLCLPMVL
                     VETAFLGTLGTTPGKAMLGVSVRDYRGRRLSFPTAFRRSLFVMVLGLGCFAPSLMLLA
                     LFFSWWWVRRFGFTPWDRKLGTTDVLNSPLTPRKVVMTLALVILCLQLIYVLLIPWLP
                     EMEAYAAASGQM"
     gene            7347..8126
                     /gene="lpxA"
                     /locus_tag="PK077_03530"
     CDS             7347..8126
                     /gene="lpxA"
                     /locus_tag="PK077_03530"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HRN23399.1"
                     /translation="MPEIHPTAVVHPAAEIADDVKIGPFCVVGEHVKLGPGCVLHSHV
                     VIDGPSSFGSGNEFFPFSVIGLKSQDLKYKGEPTYLEVGDNNVFRENATINRATDIGG
                     ATRIGNNNLFLVSCHAGHDCQIGNHVIFSGFATAAGHVTVGDYAILAGCCAVHQFVSI
                     GEHAMVGAMARVSQDVLPYTIVEGHPAVTRSVNSIGMQRRGFSEEDLKAVRMCYKKLF
                     VNKKLTVHEALEELRHSGYAENACLRRIIQFVETSERGFCH"
     gene            8131..8832
                     /locus_tag="PK077_03535"
     CDS             8131..8832
                     /locus_tag="PK077_03535"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRN23400.1"
                     /translation="MKTGFVFDLDGTLVDSIPGIARGLNLALKALGYPEHSVDAIREM
                     VGKGARELCLASLKGYFNGNVPEEAFEAVHQGFMREYPHTWQEGTVPYPGIREMLLSL
                     AGEGYPLGVLSNKPHAVTVPLVRHILPEIPFREVMGFSERFPRKPEPDSLLSIVRSWG
                     REPAQVCMVGDSAHDGNTAVNAGTRLVLVGWGYSSRSALDAFRVPVCAAAGELSARLH
                     DESSLPFPVAAHKNS"
     gene            complement(8988..10241)
                     /locus_tag="PK077_03540"
     CDS             complement(8988..10241)
                     /locus_tag="PK077_03540"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRN23401.1"
                     /translation="MISPFFPWFLAFLLLIACVALLVLLLRYRDRSVRLEAESRSWQE
                     RAEDRTQMLRRAEEVLKDSFAGISAEVLRNSEKQFLNLAETRLQSQRQQANHDLDSRK
                     QAIESMLKPVSESLKLVQSRLGEMEKERVGAYTDLKTQIRYILSGNEALKNETARLSQ
                     ALHHNSVRGQWGELQLRRVVELAGMVEYCDFTTQETRSREEERIRPDMVIHLPGGRSI
                     IIDAKAPMASYLNAGQTENPEERSQWMARHAADVRRHLQQLSAKNYFAQFSPCPEFVI
                     MFLPGESFFQAALEADPTLIEFGAENRVILSTPSTLIALLKAVAYGWKQEQLADNAKK
                     ISEAGADLYNTCSILSGHFSSLGKSLNQAVAQYNKTVSSFEHRFIPRAQKLKNLGVTS
                     TKELPRALEDITEQAKSADVPDIPS"
     gene            complement(10259..>11210)
                     /locus_tag="PK077_03545"
     CDS             complement(10259..>11210)
                     /locus_tag="PK077_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRN23402.1"
                     /translation="ISYGEIRGAANWSPRNSDGTYRGMKPASFGLILSRNTMSVRIGN
                     RAGLSNVIQSAQLAGFHGNISRTPALYLGTWEASPLDIASAYSVFANGGVRPTPYIID
                     HITDSQGQPRFAITKSKRTVYSQRAANITSSILQQVCKPGGTAGKITALGFKSPCGGK
                     TGTTNNYTNAWFAGYTSNLTCSVWVGFDSAIKILEKGYGGTLALPVWVDIMLAAQKEG
                     YPANAIRTRPGSEGQAVLVCRESNQLAHSGCQYAKTAYFETSAGYQAPANMCEQHIPM
                     AEPDSEESIPYAEPLDGSDDNIPLAEPVEETDGIPYATPI"
BASE COUNT         2104 a         2935 c         3535 g         2636 t
ORIGIN      
        1 taagcgtccg tgccgagggt ggccgcagca agttcgcttc tcccgttgta ggagaagacg
       61 tcgcggcgtg tggttccctg gcggaggagg gtgcggacgg ttggccgccc cagggcgtca
      121 taggcatagg cgcggcggga gacgagggtt gttccgcggt tatagtccat gtccgccagc
      181 aggtcacgtt tggcttcata gctttgttcc agcgtcatgc cgttgggtat cgtcaaggtt
      241 tccagcagat tgcttcctgc cagatagtta tattcaaaga tgcggttctg gccgctgtgc
      301 atgaagcccg cgctgttgat gcgcccgtcc ggcgcgtagc cccaggaggc gatttgggca
      361 ttgccgctgg cgccgcgttt gaggatgtag ccgatgctgc gaccgtaacc gtccagggtt
      421 tccacgagag ggaagacggc gccattcacc gtgacgctgt cctgtacggg gtcgctgtat
      481 tcattgtaag agaaggtacg tatgcctgag gcgtccgtta cttgcgtgag ctggccgagg
      541 atgttgtagg agaaggcctg ttccggcgtg ccgtcggaaa agccgattct tgtgagaagg
      601 ccttgcgccg cgttccatgt ataggtagtg acgatgccgc gcgcgtccgt ttccgtggca
      661 aggcggttgt tggcatcgta ggtccggacg gttccccggc cgttggcgta agtcttgccc
      721 aattccagcc cggtagcgtc atggtaagtc cagacggtag tgtctccgcc ggaaagatcg
      781 gatgggtcgg aaatgatcgt ttcttcgtct atgcggaagg tggtgaggct gataaggcgg
      841 tctgcgtcat cccaggcgaa cagggccggc tggatggccg tgccgtattc cgctgtttta
      901 cggttcctga tatcgtaggc atagttggcc gtgttgccca gggcatttgt gacggcagag
      961 ggattgtcgc tggtcgcgca ataggtcgtg gttgtcgtat tgccggcagc atcagttacg
     1021 gcaatcgggc ggccggcaat gtccgtatga gtggtggaag cgttccccct gccatccgtg
     1081 accgtccgga cgagaccggt ttccgtgtag cggcgggtga aggcggtgac gattccccga
     1141 ttatccgttt ctgaaattgt gaagccgtcc acgacggtag cggaagctgt aacgtcggag
     1201 gtgggcacgc tgcttttttc cacgcgcacc gcgccctgcc catattcggt ccaccggatg
     1261 ctttcatggc cgcgttcgtc gatggtgatt tgcctgtttt gcagaacggc ggattccgta
     1321 accagccgtt tgacagtgct gcttaacatg ttgcccgctg tattgttgcg ggcagcggcg
     1381 acgttttgat agacgccgtc ttcacgttct tcccaggctg tctggaaagt ctggatgcgg
     1441 ttttttgccg catccgccgg agacgccgag tcgagcacaa cggtttgtct ggccggattt
     1501 cccatgctgt catactcata ctccaccgga gcctgggtcc cttccgtgcg gcgggtgagc
     1561 tggcccagcg cattgtaaac gctgcgggta tagagatagg tgttttccag ggtggtggga
     1621 gaagtcagcg taatggtttg tccgaagccg tcccgaatct cctgtttcag gatggttgtg
     1681 ttgtccgcca gccgggtagt gtggcgcagg cctctgataa aatcataaat gtggtagagc
     1741 tccctttgcc ctgtgccgga ttcatgcgcg atgctcccgt ctgcgttgta tgtattgacg
     1801 agcgtcgccc ctgatggcgt ggttgtcgta accgtcagtc cgtcttcgct gtaactggtt
     1861 gttgtgacgc ggcccagcac atcggttttg cgggtcacgc gacccagcag gtcataggca
     1921 gtttcctcgg ttgtccgcat cggcccgatg tgggtcgtcg cgcttattat ctgccctgcc
     1981 gcatcgcgtt catacgccgt gatcgtttcc ggagtgacga cgtcagtccc gtcgtttatt
     2041 tcgctgcgcg tggtttcaat gagctgccgg gcagtgttat aagcgtaatc cgtgcgtacg
     2101 ccgtcttcgt caacttccca aaggggttct cctgtgcagg tcagggcgcg ggaagaagtg
     2161 cggccattgt cgcgcaccgt ttccacacgg cggttgagaa ggtcgtaaga gtaggaagcg
     2221 ccgccggttt gcctccactc gcccgtgggg agcaggacga ggttttcttc ccttgcggtg
     2281 tttccttcag cggtgatgta gctgacgcgg cgggttgact ggccgggaac gggttctccg
     2341 ttgatgcgcg tttccgccgt gacgctgtag agggccccat gatctgtggt tggggcgtag
     2401 gtataatgag tctgtacgcc gttgacggcc tgttccatct tgaggcgtcc cgccgcgaag
     2461 gcattcggcg cgccggccag ccaggtttcc gtaacgctga gctgcgtgac ggaagagcct
     2521 gccgccgttt cacgcttttc cacgcgtttg acatggtttt cttccgtata ggtatatacc
     2581 tcaaggctta atgtgttgac tgcggaaacg gtagagagct ggatttttat ttcagccggt
     2641 tcactggtgt agcctgcggc gctgtcacgg tactttgtgc ttactgtccg caatccgttg
     2701 gctccccatg ggctgttttc cagcaccggg cgtcctgcgg cgtcgtagac ggttttgtgt
     2761 tcccccgcgt tgggagaagc agaggagata agccggccgg agtcatcata ggagtaaaca
     2821 gtagtcaacg ccccttcggc tccgtaccct tccgtgcggc tcagcaggtg ttctccggtg
     2881 atggtcgttt catattcctc aagcgtgcag gaggccacca cattgttttt catcagacgg
     2941 gtgaggacgc ggtagcggtt ttcctgcgaa tcttcctccg gttgaaggcg ttccctccgc
     3001 gtttcaacgg catcttctcc tgttccttcc gccatgctcc aggcttcttg cgaccatgtc
     3061 catttggtca cgaagtcggg tacagtggcc ggcaacgtgt tgtcgcgttc agagatgacg
     3121 agggtttgca aagcttcgtc tcccgtcacg gtgaagattt tcgccggagt cccttcaacg
     3181 gtgaacaggg ctccttcttc ctccggttcc atgacctgcg cgggcggata aatgctgatg
     3241 gcataccctc ctccttccag ggggagcaca tccgccagcc catcccaggt gttccatatt
     3301 tgacggataa cgccgtgttc gccacgaaca atcgctaggt aattttcggc ttccgatgcg
     3361 gtcatcaaat acccgcccgc ggagatatag ccttcgcatt gccctgtgga aagagagtaa
     3421 aagacggagc ctcctcccgc ataagaagcg cggatgaagg cggcctgctc aaggttgcat
     3481 gcctccgctt ccctgctcat ggccgggacg ggggccagga tggtcgttgt gatgcccgat
     3541 gcgcctaccg ggaaaaacgc gcttcccgtg ccgtcaagca tgtagttggt gtaactggcc
     3601 ccatccagca aagcaaccag cgtgttgggc gccatgcccc cttccggcag cgcgaggaag
     3661 gtggctagcg gatgccgcag ggccaaacct gccggggaga ataaatccgg ggagaaagag
     3721 cggtgggctg aaatttccag acgtcccgcc ggcatgtggg atatgccgcg gaacgcgccg
     3781 aactgcgctg accaggccat gccattaacg gaggattgcc gcatcacccc gcttcctccg
     3841 gaggttgtgg tcagattgga aaagaagtgg cgcagggagg agcggccgct gactggggcg
     3901 ctgctgctgg aggggacgcc tccgggagta gtttcaccgt ccgaattgca ggagcacggc
     3961 gggcaatcac aatcgcattc ctgcggcggg tcgttctcat caggcgtgat ggagatggcg
     4021 ctggccgggc ctacggtgca attgaggaag gatacattcc ccctgggagg atagtcaatg
     4081 ttgatgtggg agacggatac tgtataaaat ccggccttga ggtcggagaa gctgccgtta
     4141 attcttactc cggcgcctga gggtgacgta tagctttgcc ccgctactga aatcgtggcc
     4201 tggtcatcca ccgtgccggt ggccgtcagt acgtacagac gggtatcccc tccgccttcc
     4261 cctacttgaa tttgtttgga ggtttccggc atcacgtctc cgggatagga cggagcagca
     4321 tgattatagg aaaaggagac ggacgattcg ccctccctct tctgtaaaac cggcgtaacg
     4381 gtaaaaccaa gatgatgatt gccccggacg ctgttgcaga tggaaaagtt agatttcatt
     4441 ctaattggct atctttaaat aatttctgtt tatcaacaat aattttgtga taaggtgcgc
     4501 catttagcaa ttaaacctat ggatacataa ataaaaatag aaatcaacaa gatatgatga
     4561 ttttctcctg ttttttccat ccttttgcat gcagaaaata tattctggcg caggatgttt
     4621 tccgggcgcg gggcacagga aagttcccaa cgcaggaaaa gatgggatgt cccttttctg
     4681 cctgattgcg cggcagaaga aggctgcccg ttttccggaa catgttttag gcttggaaag
     4741 gggagcgcaa tcaggcaagg tgtgccgcgt tattcatgaa gaacctattg agccggtaca
     4801 tcagcctgag cctggcgctg cggtatttga acccgttgcg gactttcttt tccattatta
     4861 ccctgatttg cctgctgggc gtctccctgg gggtaatggt gcttatcgtc gttctatccg
     4921 tcatgggagg cctccagaag gaaattcagg gtaatctgtt tgcgcactcc ccgcacgtcc
     4981 aggtatgcta ccggaacgac ttcggcgtta gggaagtgat tccggactgg ctggatttgg
     5041 gggagaaact caggcacgtt cccggcgtcc agtccgccta cgccctgata gaagactatg
     5101 cgctggcgga tgtgcagggg cggcagaggc cctgcttttt ccgggccatt gatacggaaa
     5161 acgccgccca gttggaggat ttgaagcgtt tggtggtcgc cggcgatgcg gaactggaca
     5221 tgggggaaaa ggctgtggtt tcctccatcg tggcggagaa catgggattg aaggtgggag
     5281 atgttgttcg ggtttacacg acccgcaatt ttcaggaaat ttcccacgcc taccagcaga
     5341 cggagctgcc gatgctggcg gagaaaaacg cgcgggaact caaggatttg aaggagtggg
     5401 gtaaatccct gaagacggag ggagagcgtg aaacggccga cagagcctcc gtggacaggg
     5461 ccttttccct gcttaacggg ctgttgtccg tcccgcgcag ggatgcggaa cgttccagcc
     5521 tgatggatct gcttgccgtg ctgaacgacg gggaaattgc ggacaacggc agggtggctt
     5581 ttccgcaagg tacgcggaaa gaatgggaaa ctgttttgga cggtttcaag gccggggcaa
     5641 ggaatgaggc ggatatggaa tgtttccgca agatcaagga actggtgatg ccgaaggacc
     5701 tggaggtgat tggaatttac cgggcctccc agcatacgcc cagcccggat cttttcattc
     5761 cgctggtcat cggccaggaa ttgctggggt atgaggatga cgtggtgcag gccgtggccc
     5821 tgcgcgtgga agatccttac catgtggaaa cgatgctggc tcccgtgatg caggcgctgg
     5881 aaaaggagaa gccttcttcc gcatggacgc tggaaacctg gcatgaccgt tttaacgcgt
     5941 ggtttgaact gatgcagaag gagcgcatga tgatgagctt tgtcctgtcc ttcatctccc
     6001 tgatttccgc tttttgcatc atggcggtga tgtttaccgt ttccatccag aggaaaaagg
     6061 aaatcgccgt gatgaaggcg ttgggagcca ctccgttcca ggtggtgcgc gtcttcctgt
     6121 ggcagggcgt catcatcggt tttgtgggag ccctcctggg ggtagggctg gggctgctgg
     6181 tgctggaata ccgcatgcag attcagggtt ttcttgcggg aataggcttt gacccgttcc
     6241 ccgtggcctt ccacggcacg gcgaatatcc cggtggtgat tgactgggcg gagctggcat
     6301 ggcaggcggt gaaagctttt gtcatggttg tggtggcatc catcattcct gccctgatca
     6361 cggcgcgcca ggatccggcc cgctccctgc gcagtatgta acaggaatcg cctatggata
     6421 tttactggat tcagggtcat gaacagaagg ggcctctgcc ggaagtggag gtcatttcca
     6481 tgctggaggc gggcctcatt ccggaaaacg cccgggcgtg gcatgccggg tgcccggaat
     6541 gggtatgtat ccgtgacctt ccggcattga agggggcagg tgccgtcagc aggggagaag
     6601 gagaacggcg gaaccgggag gaagagggac tggaaaacgc aggggaagag caggctccgg
     6661 cgcttgccgc ggatgcggat tgcccgcagg aagaaggggg gacggcctcg gaggagggag
     6721 cgccgctggt ggttccgtat gcgtacgtcc gttttctcgg cagaatggcg gatatcatga
     6781 tgcacatgac gctgtatttg tccgtgctca gagtttccgg cctggctttc aatcccggtt
     6841 tccttcctgg ttcctatgaa gctctgcttt acctctgcct cccgatggtt ctcgttgaaa
     6901 ctgcctttct tggtacattg ggaacaaccc cgggaaaagc catgctgggc gtttccgtca
     6961 gggactaccg gggaaggcgc ctttctttcc ccacggcatt ccggcgctcc ctgttcgtca
     7021 tggtgcttgg cctggggtgt tttgctcctt ccctgatgct gctggccctg ttcttttcct
     7081 ggtggtgggt gcgccgcttc ggcttcactc cctgggacag gaagctgggg actacggatg
     7141 tgctgaacag ccccctgacg ccccgcaaag tggtgatgac gctggcgctg gtcattctgt
     7201 gccttcaact catctacgtt ctgctgattc cctggctgcc tgagatggag gcttatgcgg
     7261 ccgcttccgg ccaaatgtga taaaaaggcg gccgtgcggg gtttaggctt ttaatcacct
     7321 gaatttgttt catacttgtt tcagccatgc cagaaataca cccaacggca gtagtgcatc
     7381 ctgctgcaga gatcgcggat gatgttaaaa tcggtccctt ttgcgttgtc ggggaacatg
     7441 tcaaactggg gcccggctgc gtgctgcaca gccatgtggt tatcgacggt ccttcttcct
     7501 tcggcagcgg caacgaattt ttcccgtttt ccgtcatcgg cctgaaaagc caggatttga
     7561 aatacaaggg agagccgact tatctggaag tgggggataa caacgtcttc cgggaaaacg
     7621 ccaccatcaa ccgggctacg gacattggcg gcgcgacgcg gatagggaat aacaaccttt
     7681 tcctggtttc ctgccatgcc gggcacgatt gccagatcgg caatcatgtg attttctccg
     7741 ggttcgctac cgccgccggc catgttacgg tgggggatta cgccattctg gcgggatgtt
     7801 gcgccgtaca ccagtttgtg agcatcgggg aacatgccat ggtgggggcg atggcccgcg
     7861 tgagccagga tgtcttgccg tacacgatcg tggagggcca cccggccgta acgcgctccg
     7921 ttaactccat cggcatgcag aggcgcggct tttccgaaga agacctgaaa gccgtgcgca
     7981 tgtgctataa aaagctcttc gtcaataaaa agctgaccgt tcatgaagct ctggaggaac
     8041 tgcggcattc ggggtatgcg gaaaatgcct gcctgaggag aatcatccaa ttcgtggaaa
     8101 cttccgaacg ggggttctgc cattgaatcc atgaaaacgg gttttgtttt tgacctggac
     8161 gggacgctgg tggactccat tcccggtatc gcccgcggcc tgaacctggc cttgaaagcg
     8221 ctggggtatc cggagcattc cgtggacgcc atccgggaaa tggtgggcaa gggcgcccgg
     8281 gagctttgcc tggcttccct gaaaggctat ttcaacggaa atgtgccgga ggaagctttt
     8341 gaagcggtgc accagggatt catgcgtgaa tacccgcaca cctggcagga gggcacggtt
     8401 ccgtaccccg gtatccggga aatgttgctg tccctggctg gagaaggcta tcctctgggc
     8461 gttttatcca acaagcccca tgcggttaca gttccgctgg tgcgccatat cctgcctgaa
     8521 attcccttcc gggaggtgat gggtttttcc gaacgctttc ctcgtaagcc ggaacctgac
     8581 tccctgctgt ccatcgtccg ttcctggggc agggagccgg cgcaggtgtg catggtgggg
     8641 gactccgccc atgacggcaa cacggccgtg aacgccggaa cacggcttgt tctggtgggg
     8701 tggggctaca gttcccgttc cgctctggac gctttccggg tgccggtgtg cgccgccgcg
     8761 ggggaattat ctgcccggct gcatgatgaa agcagcctgc cgtttcccgt tgccgctcat
     8821 aaaaattctt gaaagttccg gctggggcag agttccggaa accgaattcc tctgtatcat
     8881 caagattctt tcaggcgcag gaaaacgtct gcgggggatt ctttagggct gtattttccc
     8941 ggcccggaaa tgggcaggga gggaaaagcc gcatggttat tgctctttca ggacggaata
     9001 tccggaacat ccgcagattt ggcctgttcg gtaatgtctt ccagggcgcg gggaagctcc
     9061 ttcgtggagg tgacgcccag gtttttcaac ttttgggccc gggggatgaa ccggtgttca
     9121 aaagaggaaa cggttttgtt gtactgggct accgcctggt tcaggctctt gcccagagag
     9181 gaaaaatgac cggaaaggat agagcaggta ttgtacaggt ccgctcccgc ttccgaaatt
     9241 ttcttggcgt tatccgccag ctgctcctgc ttccatccgt aagcgacggc tttcagcagg
     9301 gcaatcaggg tggatggagt ggaaagaata actctgttct ccgcgccgaa ttcaatcagg
     9361 gtggggtctg cttccagagc cgcctggaag aaggattccc cgggcaggaa cataatgacg
     9421 aattcggggc atggggaaaa ctgggcaaag taattcttgg cgcttaattg ctgtaaatgc
     9481 ctcctgacgt ccgcggcatg gcgtgccatc cactggctgc gttcttccgg attctctgtc
     9541 tgcccggcat tcaggtagga agccatgggc gctttcgcgt caatgatgat ggaacggcct
     9601 ccaggaaggt gaatgaccat gtccggccgt atccgttcct cctctctgga acgggtttcc
     9661 tgagtcgtga aatcgcagta ctccaccatt cccgccagtt ccaccacgcg gcgcagttgg
     9721 agctctcccc attggccgcg aacgctgttg tggtgcagag cctgggaaag cctggcggtt
     9781 tcatttttca gggcttcatt gccggaaaga atatagcgga tttgcgtttt caggtccgtg
     9841 taggcgccca cgcgctcctt ttccatctct cccagccggg actggaccag tttcagggat
     9901 tcgctcaccg gcttcagcat ggactcgatg gcctgtttgc gggaatccag gtcatgattg
     9961 gcctgctggc gctggctttg cagccgcgtt tctgccaggt tcagaaactg tttctccgag
    10021 ttacggagga cttccgcgct gatgccggcg aaagaatctt tcagcacctc ttccgcccgg
    10081 cggagcatct gggttctgtc ttccgcccgt tcctgccagg agcgggattc cgcttcgagg
    10141 cggacggagc ggtcccggta ccgcagcagc agaaccagca gcgccacgca ggcgatcagc
    10201 agaagaaagg ccaggaacca gggaaaaaat ggggaaatca taaggaaaaa ataagcagtc
    10261 agatgggggt ggcgtagggg atgccgtccg tttcttccac gggttcggcc agggggatgt
    10321 tatcgtcgct gccgtccagc ggttccgcgt agggaatgct ctcttccgaa tccggttccg
    10381 ccatgggaat gtgctgttca cacatgttgg cgggggcctg gtagccggcg gaggtttcaa
    10441 aatacgcagt cttggcgtac tggcagccgg aatgggccag ttggttggat tcccggcaga
    10501 cgagtacggc ctgcccttcc gaaccgggac gcgtacggat ggcgttggcg gggtagcctt
    10561 ccttctgggc ggcaagcata atatccaccc agacgggcag ggccagggtg cccccgtagc
    10621 ctttttccag aattttgatg gcagaatcaa accccaccca gacactgcag gtgaggttgg
    10681 acgtatatcc ggcgaaccat gcgttcgtat aattattcgt agttccggtt ttgccgccgc
    10741 agggggattt aaagcccagc gcggtaattt tgcctgccgt gcctccgggc ttgcagacct
    10801 gctggaggat ggaagaggtt atgttcgccg cccgctggga atagacggtg cgcttgctct
    10861 tggtgatggc gaagcgcggc tggccctggg aatccgtaat atggtcaatg atgtacggcg
    10921 tggggcgtac gcccccgttg gcaaaaacgc tgtaggcgct ggcaatgtcc agaggggacg
    10981 cttcccacgt gcccagataa agggccgggg tgcgggaaat atttccgtga aagcccgcca
    11041 gttgtgcgga ttgaatgaca ttggacaaac cggcgcggtt gccgatgcgt acggacatgg
    11101 tgttgcggga aaggatgagg ccgaaggagg ccggcttcat gccccggtac gttccgtccg
    11161 aattgcgggg ggaccagttg gcggctccgc gtatttcccc gtacgaaatc
//
LOCUS       DAOMSU010000049        11098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_338_length_11098_cov_3.108847, whole genome shotgun sequence.
ACCESSION   DAOMSU010000049 DAOMSU010000000
VERSION     DAOMSU010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11098
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_338_length_11098_cov_3.108847"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1756)
                     /locus_tag="PK077_03550"
     CDS             complement(17..1756)
                     /locus_tag="PK077_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRN23403.1"
                     /translation="MSETQNKLLKAAISLAAAGVVFFLPFASWGIQLSPIEIRVIAMF
                     VMAALFWILEPIPIWTTSVMVITLSLLCVSNGSLSFLMPERYDKAAVSSILDDAIGKG
                     INPEIVGKLKENVENRLNKKTKLDAEEVRMTLGFQLMDAYEKIDLNAQELSREGKTEE
                     AAGQESIAAQLKTAAGRLYSKEITARIQGLQFVNTMQQKSTMATFADPIIMLFLGGFF
                     LAAAATKYRLDMNLAKVLLKPFGTNPKFVLLGLMSVTALFSMFMSNTATAAMMLAILT
                     PVLALFTPEDKGRAAFALAIPIAANLGGIGTPIGTPPNAIALKALQGMGLDVSFGKWL
                     MFGIPFVIVMILIAWLLLLWLFPISQKKLELQVGGKFLRTPKAIIVYVTFAVTVLLWV
                     TGKGVHGLDSNTIAMIPIAVFAITETITKEDLKKMGWDVLWLVAGGFALGLALQDTGL
                     AKNLIGSIPFAEWSPFPLMVGTGIICLFMATFMSHTATASLLIPIIAVVGVNMGDNLA
                     PMGGVTALLVSVAFASSLGMSLPISTPPNALAYATGLVSSKGMAISGVILGILGMVLT
                     YVMMMILAQCHAF"
     gene            complement(1835..2587)
                     /locus_tag="PK077_03555"
     CDS             complement(1835..2587)
                     /locus_tag="PK077_03555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HRN23404.1"
                     /translation="MGTGRGIADITPLTLLDFPNKVACIFWLRGCNLFCQYCYNVSLV
                     RGTDSPAGDRTDYLDFLRDRVGFLDGVVLSGGECTLCPDLIPICRNIRQLGFAVKIDT
                     NGTRPGVVKTLVEEGLCDYIALDYKAPEKLFGSITGRPDLFPFFTQTLDYLINKNFPF
                     EVRTTIHSDLLGEKEINQISGDLTSRGYRGTYYLQNFFNTEETLGQIGAPERMIDLSL
                     LNTHIPIGLRNFPITEKAPGSSSGKRQPSPLT"
     gene            complement(2614..2808)
                     /gene="nrdD"
                     /locus_tag="PK077_03560"
     CDS             complement(2614..2808)
                     /gene="nrdD"
                     /locus_tag="PK077_03560"
                     /EC_number="1.1.98.6"
                     /inference="COORDINATES: protein motif:HMM:NF024984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HRN23405.1"
                     /translation="MTKQEILSKYAQDRTRCTVYTRVMGYHRPVETFNAGKQGEFHDR
                     VHFVEPGRPCSCSAQTEQPR"
     gene            complement(2860..4956)
                     /locus_tag="PK077_03565"
     CDS             complement(2860..4956)
                     /locus_tag="PK077_03565"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HRN23406.1"
                     /translation="MATSIIKRSGKKEQYQGYKIEEAIRKAFHSSGTAYNRSIYEEVE
                     AKLSHQDSAHVEEIQDMIERALFRSGYFDTAKAFITYRFLHKMQREQLGGLYSGNTYV
                     DCKQTVEEYIGQSDWRIAANSNTTYSNAGLINNTAGKVIANYWLDQVYSAREGAAHRE
                     GDYHIHDLDCLTGYCAGWSLRNLLNEGFNGVRSRVNSRPPRHFREALGQMANFLGILQ
                     SEWAGAQAFSSFDTFLAPYVFKDQTDFPRIKKDIRSFVYNLNVPSRWGQSPFTNVTID
                     WTVPRDLRDQAPFSGGEHLFEGIEDANLLALARERGANKLTELTYKHFQKEMNLINKA
                     FYEVLTEGDSTGQPFTFPIPTVNITEDFDWEGENVPLLFENAAKIGSSYFQNFIGSQY
                     TVNEHGERVPDERAYKPDAVRSMCCRLQLDLRELLKRGGGLFGSAEMTGSIGVVTINL
                     ARLGYLFKQNREALFARLGELLDLAKSCLEKKRAFVQEMYDRGLYPYTKRYLPTFRNH
                     FSTIGVNGMNELLRNFSNDTMDITSEEGRAFAEEILEFIRLRMQEYQEETGNLYNLEA
                     TPAEGTTHRFAREDQKRYPDIIQAGPVGQRYYTNSSQLPVDHTSDLFRALQLQDELQC
                     CYTGGTVFHMYMNEAISSPEACRDIVRKVLTRFRMPYLTVTPLFSVCEKHGYLRGEHE
                     YCPFCDEELLHIHRHE"
     regulatory      complement(5113..5311)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            5570..7741
                     /locus_tag="PK077_03570"
     CDS             5570..7741
                     /locus_tag="PK077_03570"
                     /inference="COORDINATES: protein motif:HMM:NF027479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HRN23407.1"
                     /translation="MLSAGTSVSGASADWNEKTIRDNLQLVAEWQAKHPKKRSPLHWT
                     YGAFYSGLVQYGLSVPEGPGLPLLRKAGEEQGWKTLNRHYHADDHAVGHAWMEMAMED
                     GNPAAAEKIRAVLDKVMNRPSSASLQFLTPGCQDRWSWSDALFMSPPVFVKLAAYTGD
                     RRYLEFMDREYKLTCDYLFDREEGLFFRDSRYFTVPAANGKKMFWSRGNGWVIAGLPL
                     ILQDMPADWPSRPFYEDLLKRLAAALKKCQSPDGSWHANLLDPDEPPLKEMSGTLFIM
                     YGMLWGVNQGYLDADEYLPSIRKAWKAACDAVSKEGALGWVQPIADKPGHYSGKDTEV
                     YGAGAYLMAGSELRKYVIGRDHPQKKTVTVTNPLGRFRPAETVSVPWPSGGSGDAAGL
                     RVFDVRHGRVIPHQLADTDGDGTTDTLLFQSNFRPGTVRDFWILENSCLGEAPSADVC
                     FSRPVPERLDDFAWENDLTAHRIYGPAVARPAPEGEGLVSSGADVWSKRAGAPVINEF
                     YKRGDYHRDHGRGLDMYNVGPGRGCGGIAVFRDGKPHVSGNWASARTLYNGPVQTAFE
                     VVYAPWDIGGGVRVAEMRRVTLDAGNRFSKVRSVLNVRGAETVKAGVGMDTGKRRNDY
                     EAVMEDRESGGLMTAWSRPRKDDGCLGTAVIVPWVPEGRAVDAEGCTYLLRKVANGEP
                     FEWYMGAVWDKASPIRSAAGWEAEARRVRECISHPLQVRVR"
     gene            complement(7793..9511)
                     /locus_tag="PK077_03575"
     CDS             complement(7793..9511)
                     /locus_tag="PK077_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRN23408.1"
                     /translation="MTPPHASAGSAERYPSAYGLSAAVCQALRAAFIPRRMRCLWAAG
                     LTAALAANPYVFNDGQSLLMSSMGIACSASLLCGTILLCLKYRFTAYVLLPAIILFNA
                     GLYMMQMRYGLVLNLSVLSSMAETNFQEACAFCTPASIAGTLLLAGFIYLTIYWSRRS
                     LHQKATWGALACIWGLYAVLLLLSIPAASYSSEPLYLYYTSDKAKGWPLVDIAMTCKL
                     ADEYITQDAGRFNTLRNLLSCAEPLSQCEAPDDLAVIFHMGESVRGDHLPLNGYHRNT
                     MPRLSREPNVVSFPHATSFGIVTRISAIGMFTDAELCRRTPGHSSFIDLFNKHGFRTV
                     RIMDLSGDSIHDYSLGILTRNCRERRQTPLQHQTPGMMQERTSLVMEESLKNFGRNRQ
                     LYIIYNNGSHMAFSYPAQAERFTPASCNMDDPKARLEETVNAYDNSIVDLDASIHRMI
                     ALLKNRPAIYFYCSDHGVALGEEGKMFQGHILPPVYRPAMFIWYSDAFASRYPDMVRA
                     LKANRLKAVSHDHIFHTLLSLASIRSEIVRNDLNLASPDARETPAPLQPETLAEWLPI
                     PAPPQP"
     gene            complement(9740..10288)
                     /locus_tag="PK077_03580"
     CDS             complement(9740..10288)
                     /locus_tag="PK077_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRN23409.1"
                     /translation="MTKTIENLKAAITGESTASATYAAYAVKAAQEGQPLIQKLFEAA
                     SKAESVHAANHNKVLEKLGATMEPIDIQIHVGTTAENLKAAIAGETHEFESMYPEFIA
                     VAEEEGQKAAVRSFTWATEAEKEHADFYSVALKALESGDTSSLPRQYWICLVCGDTFK
                     SLEGVDACPLCGTKAEKFLVIG"
     gene            10443..10991
                     /locus_tag="PK077_03585"
     CDS             10443..10991
                     /locus_tag="PK077_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23410.1"
                     /translation="MKSIVTISSACALAFAGMASAQDAPAPAPAAETPAAPVAPAAEV
                     SKEAKEAADQMLALMKEMSATLSTVKDKASADAAAEKMAKINEKGEALSKSAQKVQAE
                     MDAAMQARQAELLPIFMGMMTDMQRLQQADFYGSQALKDAMKQTGATGPEEDVEVEED
                     VEVEAVPAPAPAQKEASAPAAK"
BASE COUNT         2350 a         2803 c         3462 g         2483 t
ORIGIN      
        1 tggaacatta ccttacttaa aaagcatggc attgggcaag aatcatcatc atgacgtagg
       61 ttaaaaccat gcccaggatg cccaggatga cgccggaaat ggccattccc ttggagctga
      121 ccaggccggt ggcataggcc agggcgttgg gaggggtgct gatgggaagg ctcatgccca
      181 gggaggaggc gaaggccacg gataccagca gggcggtaac gccgcccatg ggagccaggt
      241 tgtcacccat gttgacgccg acaacggcga taatcggaat gagcagggag gccgtagccg
      301 tatggctcat gaaggtggcc atgaacaggc agatgattcc cgtccccacc atgagcggga
      361 agggggacca ttcggcaaag ggaatggagc cgatcaggtt ttttgccagt ccggtgtctt
      421 gcagggccag ccccagggca aagccgccgg ctaccagcca gagcacgtcc cagcccattt
      481 tcttcaagtc ttccttggtg atggtttccg taatggcaaa gacggcgatg ggaatcatgg
      541 cgatggtatt ggagtccagc ccgtgcacgc ccttacccgt tacccacagt aggacggtaa
      601 cggcaaaggt gacgtaaacg ataatggcct tgggggttct caggaattta ccgcccacct
      661 gcagttccag tttcttttga gagatgggga acagccacag caggagaagc catgcaatca
      721 gaatcatgac gatgacgaaa ggaatgccga acatgagcca tttgccgaag gagacgtcca
      781 gccccatacc ctgcaatgcc ttcagcgcga ttgcgttggg gggcgtgccg ataggcgttc
      841 cgataccgcc gaggttggcg gcgatgggga tggccagggc aaaggcggcg cggcctttgt
      901 cttccggagt gaacagggcc agcaccggcg tcaggatggc gagcatcatg gcggccgtag
      961 ccgtgttgct cataaacatg gagaaaagag ccgttacgga catcagcccc agaagtacga
     1021 atttgggatt ggtgccgaag ggttttaaaa gtactttggc caggttcatg tccagcctgt
     1081 atttggttgc ggccgcagcc aggaaaaagc cgcccaggaa aagcatgatg atgggatccg
     1141 cgaaggtggc catcgtggat ttttgctgca tggtgttgac aaattgcagc ccctggatgc
     1201 gcgcggttat ttccttgctg tacaggcgcc cggccgcggt cttgagctgg gcggctatgg
     1261 attcctgccc ggcggcttct tccgtttttc cttcacggga cagttcctgt gcgttcaagt
     1321 ctattttttc ataagcgtcc atgagctgga accccagggt catcctcact tcttccgcgt
     1381 ccagcttggt tttcttattt aaccggtttt cgacattttc cttcaatttg ccgactatct
     1441 ccggattgat ccctttgcct atggcgtcat ccagaatgga ggataccgcc gccttgtcat
     1501 agcgctccgg catcaagaat gagagagagc cgttggagac gcacagcagg gacagggtaa
     1561 tgaccatcac ggaggtggtc cagataggga tgggttccaa aatccagaag agcgccgcca
     1621 tgacgaacat ggcgatgacg cggatttcaa tagggcttaa ttggattccc catgaagcga
     1681 aaggcaggaa gaagacgact cctgcggcgg ccaggctgat agctgccttg agcagcttgt
     1741 tttgggtttc actcatgaat aatagctggt aatatggttg gcgggcggaa gggtacttcc
     1801 cgattttaca aatagaaact tttatttaaa aaaattatgt cagaggggac ggctgccgtt
     1861 tcccggagct ggagccggga gctttctccg tgatgggaaa attgcgcaac cctatgggga
     1921 tatgcgtgtt tagcagagat aagtcaatca tccgttcggg agcgccgatt tggccgagtg
     1981 tttcctctgt attaaaaaaa ttctgaaggt agtaggttcc tctatagccg cgcgacgtca
     2041 aatccccgct gatctggttg atttcctttt cgccgagaag gtcggagtgg attgtcgtcc
     2101 gaacctcaaa cgggaaattt ttgttgatca gataatccag tgtttgagta aagaagggaa
     2161 ataagtccgg acgcccggta atggagccga acaatttttc cggcgctttg taatctagtg
     2221 caatgtagtc gcaaagccct tcctccacca gcgttttcac tacgccgggc cgggtgccgt
     2281 tggtgtctat tttgactgcg aaaccaagct gccgtatatt gcggcagata gggataaggt
     2341 cgggacacag cgtgcactcg cctccggaca gaaccacccc atccagaaat cccacccgat
     2401 ctctgagaaa atcaagatag tccgtccggt ctccggcggg agaatccgtg cccctgacca
     2461 gggagacgtt gtaacagtac tgacaaaaca aattacagcc tctcagccag aagatgcagg
     2521 ccaccttatt tggaaaatcc agcaaggtga gcggcgtgat gtctgctatg ccccgacccg
     2581 ttcccatcga cttcgcatat gttgttggga gaattatcgc ggctgttccg tttgggcgga
     2641 gcaggagcag gggcggcccg gttccacgaa gtggacgcgg tcatggaact cgccctgttt
     2701 gccggcgttg aaagtttcta cagggcggtg atagcccatg acgcgcgtgt aaacggtgca
     2761 gcgggtccgg tcctgtgcgt atttgcttaa tatttcctgt ttggtcattg tcgtattaat
     2821 ggttgggttt gaagtcggga gcctattgga aaagggattt tattcatgcc tgtggatgtg
     2881 gagcagctct tcatcgcaga aggggcagta ttcgtgctcc ccgcgcagat agccgtgttt
     2941 ctcgcaaacg gagaagagcg gggtgacggt gaggtacggc atgcggaagc gggtcagcac
     3001 tttgcggaca atgtcccggc aggcttccgg ggagctgatg gcttcgttca tgtacatgtg
     3061 gaagacggtt cctcccgtgt agcagcattg aagttcatcc tgaagctgga gcgcccggaa
     3121 caggtcgctt gtgtggtcca cgggaagctg ggagctgttc gtgtaataac gctgccccac
     3181 ggggccggcc tggatgatgt caggatagcg tttctggtct tcgcgggcga aacggtgcgt
     3241 ggtgccttcc gcgggcgtgg cttccaggtt gtacaggttg ccggtttcct cctggtactc
     3301 ctgcatgcgc aagcggatga attccaggat ctcttcagca aaggccctgc cttcttcaga
     3361 ggtaatgtcc atcgtatcgt tggagaaatt gcgcaacagc tcgttcatgc cgttgacgcc
     3421 gatggtggag aaatggttgc ggaaggtggg caggtagcgt ttggtgtagg ggtacaggcc
     3481 gcggtcatac atttcctgaa cgaaggccct tttcttttcc aggcaggatt tggccaggtc
     3541 cagcagttct cccagcctgg cgaatagggc ctcccggttt tgcttgaaca ggtagcccag
     3601 ccgggccagg ttgatggtca ctacgccgat ggagccggtc atttccgcgg agccgaagag
     3661 accgccgccg cgtttgagca gttcccgcag gtccagctgg aggcggcagc acatggaacg
     3721 cacggcatcc ggcttgtagg cccgttcgtc aggcacgcgt tccccgtgtt cattgacggt
     3781 gtactggctg ccgatgaagt tctggaagta agaggaaccg attttggcgg cgttttcaaa
     3841 caggagaggc acgttttccc cttcccagtc aaaatcttcc gtgatgttga cggtggggat
     3901 ggggaaggtg aagggctgtc cggtgctgtc cccttccgtg agaacttcgt aaaaggcctt
     3961 gttgatcagg ttcatttcct tctggaaatg cttgtaggtc agttccgtca gcttgtttgc
     4021 cccgcgttcc ctcgccaggg cgagcaggtt cgcgtcttct attccttcaa agaggtgttc
     4081 ccctccgctg aagggggctt ggtcccgcag gtcgcgcggc acagtccagt cgattgtgac
     4141 attggtaaag ggagactgtc cccagcgcga gggtacgttc aggttgtaaa cgaaactgcg
     4201 gatgtctttc ttgatgcggg ggaagtccgt ctggtccttg aaaacgtagg gcgccaggaa
     4261 ggtgtcaaag gagctgaagg cctgggcgcc ggcccattcg ctttgcagaa tgcccagaaa
     4321 gttcgccatc tggcccaggg cttcccggaa gtggcggggc ggcctgctgt tcacgcggct
     4381 gcgcacgccg ttgaatcctt cattgagcag gttgcggagg ctccatccgg cgcagtatcc
     4441 ggtcaggcaa tccaggtcat ggatgtggta atccccttcc cggtgcgcgg ctccttcccg
     4501 tgcggagtaa acctggtcca gccaatagtt ggcgattacc ttgcccgccg tgttgttgat
     4561 gagccccgca ttggaatacg tggtattgga gttggcggca atgcgccagt cgctttgccc
     4621 gatatattcc tctaccgtct gcttgcagtc cacataggta ttgccggaat aaaggccgcc
     4681 aagctgttcg cgctgcattt tgtgcaggaa acggtaggtg atgaaggcct tggccgtgtc
     4741 aaaatatccg gagcggaaca gggcgcgttc aatcatgtcc tgaatctctt ccacatgcgc
     4801 gctgtcctga tggctcagct tggcttccac ctcttcatag atggaacggt tgtaggcggt
     4861 cccggaactg tggaaggctt ttctaatggc ctcttcaatc ttatagccct ggtattgctc
     4921 ctttttcccg gaacgtttga tgatgcttgt tgccatgatg ggtgtctcgc ttggaaaggg
     4981 tggtgaccgc tcatccgcga gcctgggcaa aagcctggaa aaaccatagg caggggccaa
     5041 ccgcatgtgc gcattcccgg aaagggaggg catgccgaag acctgtgcgt atgttcatcc
     5101 tgtccgcggg atgggaatac ggcagcatca ggtcgtcttc tggcttccgg tgcgacatgt
     5161 gatggggact tcccggctgc ctgggcagcc agtgtccggt ccatcgtcta ccggttacag
     5221 cggcgcgtcc gcgacggatt ttcaccgtct tccgttacct gcgctggaac gcaatgaacc
     5281 acagaaagag acctcccgca agaaaaatgt ataaataatg tgttaaataa ttattcatat
     5341 gctatatata gtatatgacg gtagaaaaat gattgataac acagctttct tttccgtcat
     5401 ggcaccatat atgcccttgc gtccctttcc cggtctggag gagcgcggga aaagaaaaat
     5461 tcttgttaca agaaaattcc agccttcttt tcccgtataa tataacatgc gtttctttgc
     5521 cttttcaaaa aatggatgga agaaatactt tctgttgccg gtcctttcca tgctttccgc
     5581 aggaacttcc gtttccgggg cttccgcaga ctggaatgag aagaccatcc gggacaatct
     5641 tcagctggtg gcggaatggc aggcgaagca tcccaaaaaa cgttcgccgc tgcattggac
     5701 ttacggtgcc ttttattccg gcctggtgca atatggcctg tccgttccgg aagggccggg
     5761 gctgccgttg ctgagaaaag cgggagaaga gcaggggtgg aagaccctga accggcatta
     5821 tcatgcggac gaccatgccg tggggcatgc ctggatggag atggcgatgg aagacggcaa
     5881 tcccgccgca gctgaaaaga ttcgcgccgt gctggacaag gtgatgaacc ggccttcctc
     5941 cgcttccctc caattcctga ctccgggctg ccaggaccgc tggagctggt cggacgccct
     6001 gtttatgtcc cctcccgtgt tcgtgaaact ggcggcctat accggagacc gccgttatct
     6061 ggagtttatg gatagggaat acaagcttac ctgcgactat ctttttgacc gggaggaggg
     6121 cctgtttttc cgggattccc gctattttac cgttccggcg gcaaatggga aaaagatgtt
     6181 ctggagccgg ggcaacggct gggtgattgc cggattgcct cttattttgc aggacatgcc
     6241 tgcggactgg ccttcccgcc ccttttatga agacttgctt aaacgtctgg cggcagccct
     6301 gaaaaaatgc cagtcgcccg atggttcctg gcatgcgaac ctgctggatc cggacgaacc
     6361 tcccttgaaa gagatgagcg gtaccctttt tatcatgtac gggatgctgt ggggagtgaa
     6421 ccagggatat ctggatgcgg atgaatacct tccctccatc cgtaaggcct ggaaggccgc
     6481 ctgtgatgcg gtcagcaagg agggcgcgct gggatgggta cagcctattg cggacaagcc
     6541 gggccattat tccgggaaag atacggaggt gtacggcgca ggggcctacc tgatggccgg
     6601 gagtgagttg cgtaaatatg tcattggccg ggatcatccg cagaagaaga ccgtgaccgt
     6661 tacgaatccc ctgggcaggt tccgccctgc tgagaccgtg tcggttccat ggccgtccgg
     6721 aggttccggt gatgccgccg gccttcgcgt ttttgacgtt cgtcacggac gtgttattcc
     6781 gcatcaactg gcggatacgg atggtgacgg aacaacggat acccttttgt tccagagcaa
     6841 tttccggccc ggaacggttc gtgatttctg gattctggag aattcctgcc tgggcgaagc
     6901 gccttccgcg gatgtctgtt tcagccgtcc ggtaccggag cggctggatg atttcgcatg
     6961 ggaaaatgat cttacggcgc accggattta cggtccggcc gtcgccaggc ctgcccctga
     7021 gggggagggc ctggtttcca gtggggcgga tgtatggagc aagcgcgcgg gcgctcccgt
     7081 tatcaatgaa ttttacaaac ggggggatta ccaccgggat cacggtcggg gactggatat
     7141 gtacaatgta ggtccggggc ggggctgcgg cggcattgcc gtgttcaggg atggaaaacc
     7201 gcatgtgtcc ggaaactggg ccagcgcccg gacattgtac aatggcccgg tccagaccgc
     7261 ttttgaggtg gtttatgcac cgtgggacat tggcggcggc gtgcgtgtgg cggagatgcg
     7321 gagggtgacg ctggatgcgg gaaaccgttt ctccaaagtc cgcagcgtct tgaacgtccg
     7381 gggggctgaa acggtgaagg ccggtgtggg aatggatacc ggaaagcgca gaaatgatta
     7441 tgaagcagtt atggaggacc gggagtccgg cggcctgatg accgcatgga gcaggccccg
     7501 gaaggatgac ggatgcctgg gaaccgccgt aatcgtgccc tgggttccgg aaggccgtgc
     7561 ggtggatgcg gaaggctgta cctacttgct caggaaggtc gcgaacggag aaccctttga
     7621 atggtacatg ggggcggttt gggacaaggc ttctcccatt cggtccgctg ccggctggga
     7681 agcggaggcg cgccgtgtcc gggagtgcat cagccatcca ctgcaagtcc gggtgcggta
     7741 gaagccgccg catcctgttg tttctcgcgg agcgcgtgga ggcttcctcc tgttacggct
     7801 gcggtggtgc gggaatgggc agccattccg ccagcgtttc cggctggagg ggggccggag
     7861 tttcccgcgc gtccggagaa gccagattca ggtcgttcct gacgatttcc gaccggatgg
     7921 aagccaggga aagaagggta tggaagatgt ggtcgtggga gacggctttc agccggttgg
     7981 ctttcagggc gcgcaccatg tccggatagc gtgaggcgaa agcgtccgaa taccagatga
     8041 acatggcagg ccggtaaaca ggcggcagga tatggccctg gaacattttt ccttcctctc
     8101 ccagcgcgac gccgtggtcg gagcagtaaa aataaatggc gggcctgttc ttcaacagtg
     8161 caatcatgcg gtgaatggag gcatccaggt cgacgatgga attgtcatag gcattgacgg
     8221 tttcttccag acgggccttg gggtcgtcca tattgcagga tgccggggta aaacgttccg
     8281 cctgcgcggg atagctgaac gccatatggc tcccgttatt gtaaatgata taaagctgcc
     8341 tgttgcggcc gaagtttttc agggattcct ccatgaccag ggaagttcgt tcctgcatca
     8401 ttccgggcgt ctggtgctgg agcggcgtct gtctccgttc acggcagttc cgcgtcagga
     8461 tgcccaggga ataatcatga atggaatctc cgctcaggtc catgatacgg acggtgcgga
     8521 atccgtgttt gttgaacagg tcaatgaagg aggagtgacc gggagtgcgg cggcacagtt
     8581 ccgcatccgt aaacatgccg atggcggaaa ttctggtcac gatgccgaag gaggtggcgt
     8641 gggggaagga aacgacgttg ggctctctgg aaagccgggg cattgtgttc cgatggtaac
     8701 cgttcagggg aagatggtcc ccccggacac tctcccccat gtggaaaatc acggccaggt
     8761 cgtctggggc ttcgcactgg gaaaggggct ccgcgcagga cagaaggttc cgcaatgtgt
     8821 tgaaacgccc cgcatcctgg gtgatgtatt cgtccgccag tttgcacgtc attgcaatat
     8881 ccacgagagg ccagcctttg gccttgtcag acgtgtagta aagataaagg ggttccgagc
     8941 tgtaggaggc tgccggaatg ctcagcagca gaaggaccgc gtacaggccc caaatgcatg
     9001 ccagtgcgcc ccacgtggct ttttggtgca gggaacggcg gctccagtag atggtcaggt
     9061 agataaaccc ggccagaagc agggttcctg caatggaagc gggggtacag aaggcgcagg
     9121 cttcctggaa attggtctcc gccatgctgg aaagcacgga aaggttcagg accaatccgt
     9181 accgcatctg catcatgtac aggccagcat tgaacagaat gatggcggga agaaggacat
     9241 aggcggtgaa acggtatttc aggcagagaa gaatggtgcc gcagagcagg gatgcggagc
     9301 aggcgatgcc catgctgctc atcagcaggc tttggccatc gttgaacaca tagggattgg
     9361 ccgccagcgc cgctgtaagc ccggctgccc acaggcagcg catgcggcgg ggaatgaacg
     9421 cggcgcgcag agcctggcag acagcggccg acaggccgta tgcggacgga taccgttctg
     9481 cggaaccggc ggatgcatgg ggaggggtca tcgcaaagga gggataaaga atgcttcctg
     9541 cttaaaggga agaaaaatta taagagtttt aaaatgaatc gcaagcagaa tgttggagaa
     9601 gctttttccg gtgtgggccg cctccgtaaa aagggaaggg ttttcttcca tggactgctg
     9661 cggcaaaaac agaaaaccgc ccggaatgtt ccgggcggtt cctgggtgcc gtggacggcc
     9721 tccggtgctc aaacggagtt tatccgatga cgaggaattt ttcagccttg gtgccgcaga
     9781 gggggcacgc atccacgcct tccagagatt tgaacgtgtc tccgcaaacc aggcatatcc
     9841 agtattgcct gggcagggaa ctggtatctc cgctttccag ggccttcagg gcgacggaat
     9901 aaaaatcggc atgttctttt tccgcttccg tggcccacgt gaaggaacgg acggcggctt
     9961 tctgcccttc ttcttccgcc acagcgatga attccggata catgctttca aattcatggg
    10021 tttccccggc aatggcggct ttcaggtttt ccgcggtggt tcctacatgg atctggatat
    10081 cgatgggctc cattgttgct cccagctttt ccagaacctt gttgtggttg gcggcgtgga
    10141 cggattccgc cttggaggcg gcttcaaaca atttctgaat aaggggctgc ccttcctggg
    10201 cggctttgac ggcataggcc gcgtaagtgg cggaggcggt gctttcgccc gtaatggcag
    10261 ctttcaggtt ttctatggtt ttagtcatcg gaaataaatt tataataatt ctaaaaagat
    10321 gcaactgaaa aaatccttca agggaggaaa aaacgcaaat tcctctttct gcggcatcac
    10381 ggggagccgt tacacaatga ttcattgacg cgtgcatggc gagcctgtac aacggaaacg
    10441 ttatgaagag cattgtaaca attagttcgg catgtgccct ggcatttgca ggtatggctt
    10501 ccgcccagga tgccccggct ccggcacccg ccgctgaaac tcctgccgcg cccgttgcgc
    10561 cggccgctga agtttccaag gaagcgaagg aagccgctga tcagatgctg gcgttgatga
    10621 aggaaatgtc cgccacgctc agcacggtga aggacaaggc ctctgcagac gcggctgcgg
    10681 aaaagatggc aaagatcaat gagaagggag aagccctttc caagagcgcc cagaaggttc
    10741 aggctgaaat ggatgccgcc atgcaggcgc gccaggccga gcttctgccc attttcatgg
    10801 gcatgatgac tgacatgcag cgtttgcagc aggccgactt ttacggaagc caggccctga
    10861 aggacgccat gaagcagacc ggcgctaccg gtccggaaga agatgtggaa gtggaggaag
    10921 atgtggaggt agaagcagtt ccggcccccg ctcccgccca gaaggaagcg tccgcccccg
    10981 ccgccaagta actttctttt tcaggattgt ctctcctccg gcattgcgag atgccggagg
    11041 atttttttat ggcagggccg tctgaaaaag cctccggggg gtgacgcagg aacctgcg
//
LOCUS       DAOMSU010000050        11009 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_343_length_11009_cov_2.487950, whole genome shotgun sequence.
ACCESSION   DAOMSU010000050 DAOMSU010000000
VERSION     DAOMSU010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 11009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11009
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_343_length_11009_cov_2.487950"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..600)
                     /locus_tag="PK077_03590"
     CDS             complement(<1..600)
                     /locus_tag="PK077_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HRN23411.1"
                     /translation="MLRVIALLDKPEIKALRLGGEAWYEIDDIQDLDIAASIFTPDRE
                     EHLRLMESRYGGYWRYPRIRDFCYMVNPYFPNKRLMSELKANFERLVREYPSGMRVNS
                     LLAAKYFGISQEYICIGNGAAELIKALMSRLEGKMGVIYPTFEEYPNRLNPDMVVPFH
                     TASRNFTYTADDLMEFFSGKDIGTLLLVNPGNPSGFFLPK"
     gene            1013..2032
                     /locus_tag="PK077_03595"
     CDS             1013..2032
                     /locus_tag="PK077_03595"
                     /inference="COORDINATES: protein motif:HMM:NF016852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HRN23412.1"
                     /translation="MMKLPSFITAGRTGKLVRGKHWWVTEVRCCGLNVLSSCTDRKKR
                     FVRLFGIPCGRFKVGRESEFSQLGKLVKATVRAEEMPQASGVLRIVQQAMTCFLNEVA
                     GILEEGGYQYWLDFGTLLGAVRHRGFIPWDDDLDISMLREDYERLRANAAGLFGSRGF
                     SVSMDPFIQIGLPGTLCNVDIFPYDTAPAAWSPDAPEEEEWLLRGYKASGMLDYEADS
                     SCRYRTRCSYEEKMAIRDRVVMGGRRPADGGNIFLGSEIPFAGPCRHSFRHEWLFPLS
                     RVRFEGREFPAPRLPEMVLYGQYGDWGALPDSPPVHFDLNHISKEVLARMMYMRDTGR
                     LPGRD"
     gene            2115..4154
                     /locus_tag="PK077_03600"
     CDS             2115..4154
                     /locus_tag="PK077_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HRN23413.1"
                     /translation="MMRRPPIPGLVMADGWLQPYSRQIRDRQRLFDLKMKRIHQRAGS
                     LEEYARGYRYYGFNRDAETGAWTYREWAPAARRVSLIGDFNGWNRESHPLERNERGVW
                     EITLPPDALAHGQKVKVHVVGADGTGRDRIPAWITRAVQDPSTYDFAGEIWMPEHPYE
                     WWNNGFDPSRVEVPFVYEAHVGMGGEEGRVHTYREFADEVLPRISRLGYNTVQLMAIQ
                     EHPYYGSFGYHVSSFFAPSSRFGEPEDLKYLIDQAHGLGIAVLLDVVHSHAVKNEAEG
                     LNNFDGSGGMYFLPGERGRHPDWDSCCFDYGRDEVLEFLLSNVRWWLEEFRFDGFRFD
                     GVTSMLYFHRGHEPFGDLGAYFGSSVDLDAVAYLQLATTLIQRVKPGAIAIAEDMSGM
                     PGLCRPVDEGGIGFSHRLAMGIPDYWIKLLKEKKDEEWSMGDMWHTLTNRRYGEPHVA
                     YCESHDQALVGDKTLAFRLMDAEMYWKMAVDQQSLIIDRGMALHKMIRLVTLATGGEG
                     WLNFMGNEFGHPEWIDFPREGNDWSYEYCRRQWSLVDNPSLRFKFLNAFDQAMVRLAQ
                     EARLLNNPPPFPLNIDETNHVMAFHRGGLVFVFNWSGDRAIMDYMLPVPQKGEWRVVL
                     DTDNARFGGFGRQDVSMPHFTDGDGNLSLYLLPRTALVLKRVGSAVMARRLGRED"
     gene            complement(4281..4661)
                     /locus_tag="PK077_03605"
     CDS             complement(4281..4661)
                     /locus_tag="PK077_03605"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRN23414.1"
                     /translation="MTHGLFPHLLLLSAFCGASLSCSSSSRDITIRSIPSGASLRVNG
                     DYAGQAPVTLSLNRHKPVHVAADKPGFLSTEKTFYPEMTTQGAILWGRNNEKSKDFKT
                     DILTIRLKKADSGVPPLRELPAQW"
     gene            complement(4675..5094)
                     /locus_tag="PK077_03610"
     CDS             complement(4675..5094)
                     /locus_tag="PK077_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HRN23415.1"
                     /translation="MNYEERLQDLLDELDLFQDWTERYEYIISLGKKLPRLDEAYKTE
                     DSLIKGCQSRVWLHTEPDNGVLKLYADSDSLITKGLIAVFIRLLSGLPPEEILKADMS
                     KLDKTGLKDHLAPTRANALNSMAAQIKQAAIKMAEHH"
     gene            complement(5128..6045)
                     /gene="argF"
                     /locus_tag="PK077_03615"
     CDS             complement(5128..6045)
                     /gene="argF"
                     /locus_tag="PK077_03615"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRN23416.1"
                     /translation="MNNLLSIEQLTGDEIRDLLALGHRLKAERGHHERLPLKGQTWAL
                     IFSKSSTRTRVSFEVGISELGGRPMFLSVHDIQLGRGEPIKDTARVLGRMIHGAAIRT
                     YGQQEVEEFASFSGIPTINALTDEEHPCQILADLLTIEEIYGPGSWKDMKIAFVGDGD
                     NNMSRSWMWAAKRLGFTLAIGAPTNYLPLEDFRRHLDCENVIFTTDPVEAVKGASVIN
                     TDVWLSMGQESEGLSKEKHFYPYQVNRELLEHAASGHSVFHCLPAYRGKEITEDVLEH
                     FAPVIFREAENRVHAQKAVLATLADARRG"
     gene            complement(6148..7335)
                     /locus_tag="PK077_03620"
     CDS             complement(6148..7335)
                     /locus_tag="PK077_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRN23417.1"
                     /translation="MEKPFTQVLGLPALLKKQGYTTIHCGKAHFGSKNTPGANPRLFG
                     FDYNIAGTEIGGPADYRGSRKYGTGNFHVRGLDENNYYENDTFLTEALTQEALKRLDA
                     IRKNPREADKPFYLYMSHYAIHAPFDIRGYDKRFAEEYSNPNDGHKWSDNEKRYSALI
                     QGMDKSLGDIREYLKKNNLDKNTVIIFMADNGGLAISGRMGNKESNYPLSFGKGSNRE
                     GGIREPMIVYWPGVTKAESVCATPVIIEDFFPTILEIAGAKKIQAPQVVDGKSFVALL
                     KGGSMNPNRPLLFHTPNVWGEGNGNNSRYSPSTAMRQGDWKLIYWHPDQKFELFNLKE
                     DISEEHNLAEQQPERVKAMARTMTALLKERKAQMPTYKKNNPTGAREGAPVPWPDQAA
                     ARL"
     gene            complement(7359..7844)
                     /locus_tag="PK077_03625"
     CDS             complement(7359..7844)
                     /locus_tag="PK077_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRN23418.1"
                     /translation="MRYYKYRSFLVMNISRIAAFLIPFLLGSTCSASAASVKASRAAE
                     PKRPNIILFLVDDMGWQDTSLPFWREEDGTPKPTFLNKRYRTPNMEALGKQGMVFTNA
                     YAQPICSPSRCSLMSGMNSARHRVTNWTQATKRSRPPQTGASTASSRQGPRHPEPPIF
                     P"
     gene            complement(7941..9719)
                     /gene="ptsP"
                     /locus_tag="PK077_03630"
     CDS             complement(7941..9719)
                     /gene="ptsP"
                     /locus_tag="PK077_03630"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HRN23419.1"
                     /translation="MIQEPISTEETWLQGIPVSPGIALGKIKIQISGSKEPVAYDISP
                     EEVDAELVRFHRALQTTADQIRTLRERMIQISGEKDASIFDAHILLLQDKIILQQVQT
                     ELLKRLQNVEHIFYVVMQNYMEVLRRVDDPYLRAKTADMEDVMQRVINNLRSTEPPED
                     EEETEKDQVLVAYDLTPSDTAAMDASLIHGFATEIGSSVSHTAILARSMGIPAVVGLD
                     QALLRVESHSPAILDGYKGVLILKPTKETEEYYHRLQVEKEKAYKALEALRDLPTITR
                     DGRNIRLSVNVEFPHEYSGIKEVGAEGVGLFRTEFFLLSNPSGMPDEEEQMRYYRKLA
                     EGCSPHGVIFRTLDSGGDKLPCEQLRTPEPNPFLGWRGIRVSLSRPELFKQQLRAILR
                     ACADTPGCGIMFPMVSGYTEVVTAKHILQECREELERKNIPYARDLKVGIMIEVPSAA
                     IMTDVLAREVDFFSIGTNDLTQYTIAVDRVNNRVANMFRPTHPAVIRIMDMTITAGER
                     ENIPTAICGEMAGDITLLPLLIGLGATSMSVGVHLVPIIRYAIRNLDYGQCRDMAQKA
                     LQAPNSRAIVDLSTALARKSYPALFE"
     gene            complement(9766..10032)
                     /locus_tag="PK077_03635"
     CDS             complement(9766..10032)
                     /locus_tag="PK077_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HRN23420.1"
                     /translation="MVTKELTIINKLGIHARPAAQFVKLASKFDADIVVEKDGEEVDG
                     KSILGLMMLAVGHGSKITITAEGKDEQEALNALEDLISRKFEED"
     gene            complement(10117..>11009)
                     /gene="hprK"
                     /locus_tag="PK077_03640"
     CDS             complement(10117..>11009)
                     /gene="hprK"
                     /locus_tag="PK077_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HRN23421.1"
                     /translation="ALQLELINSPAGLSRHICEPALNRPGLAIAGFYSYFANKRIQVF
                     GSAELAYLQKLPEGMRKSRIQRMFRCEVPGIVFSRDQNPPREIVELADEAGVCVFRTS
                     LVTMKFVNSATIILENEFAESVTLHGCMVDVRGVGVLIRGKSGVGKSETALGLIERGA
                     ALVADDMVYVRNVGGELVASAPEMSRGFMEVRGLGIVNITTLFGLKSIRHNKRLDLIV
                     TLIPAKDQEELDRLGLEREGLDVLGEKVLHVQLSVAPGRDIARLVEVAAMDYHLKDMG
                     IDMAGEFNRRLMSNFQSSEN"
BASE COUNT         2050 a         2867 c         3473 g         2619 t
ORIGIN      
        1 tttcggcagg aagaagccgg aagggtttcc cggattgacg aggagcaggg taccaatgtc
       61 tttcccggaa aaaaattcca tcaggtcgtc cgccgtgtaa gtgaaattcc gggaagcagt
      121 atggaagggc accaccatat ccggattcag ccggttggga tattcctcaa aggtagggta
      181 gatgaccccc attttcccct ccaggcggga catgagcgcc ttaatgagtt ccgccgcgcc
      241 gttgccgatg cagatgtatt cctggctgat gccgaagtat tttgccgcca gcaggctatt
      301 gaccctcatt ccggacgggt attccctcac caggcgttca aaatttgcct tgagttcgga
      361 catgaggcgt ttgttgggga aataggggtt gaccatgtag cagaaatccc tgatgcgggg
      421 atagcgccag tagcctccgt accgggattc catcaggcgc aggtgttctt ccctgtccgg
      481 ggtgaatatg gaagctgcga tgtccaggtc ctggatgtcg tctatttcat accatgcttc
      541 gcctccgagg cgcagcgcct tgatttccgg cttgtccagc agggcgatga cccgcagcac
      601 ctgttcgtaa tattcattat tgcccagggc tttgctgtag gctgtaagaa aggggacgta
      661 atgggtccgg gagaattccc tgctgaattt gtaaatattg acggttttaa aatattccgt
      721 cttttctttt tacccgtgta atattccatt aacatattct tacagaaatt tttactttga
      781 cggcggatgc tcaatccgct tgtcgccaat acagtggaaa ggggcggatg aggtttgtcc
      841 gccattttgg aaaaagcagt tttaagatga tggaacgggt gtttttctcc gaaaagaacg
      901 gcttgttttt cattgacttg taagaagtgg gagataaaga ttctgaatct gccggatgtg
      961 ctttcctgga aaaatgaagc atagcccgcg tgttttttga ccaaatttac ctatgatgaa
     1021 gctgccatca ttcattaccg ccggaagaac cggaaaatta gtaaggggaa agcattggtg
     1081 ggtgacggaa gtccggtgct gcggcctgaa cgtgctttct tcctgcacgg acaggaagaa
     1141 gcgtttcgtc cgtcttttcg gcattccgtg cgggcggttc aaggtgggcc gggaaagtga
     1201 attttcccag ttgggcaaac tggttaaagc taccgtccgg gccgaggaaa tgccccaggc
     1261 atccggagtg ctgaggattg tgcagcaggc catgacctgt tttttgaatg aggtggccgg
     1321 cattcttgaa gagggcggtt atcagtactg gctggatttc gggaccctgc tgggcgcggt
     1381 gcggcaccgg ggctttatcc cctgggatga cgacctggat atttccatgc tgcgtgaaga
     1441 ttatgagagg ttgagggcga acgccgccgg gcttttcggt tcgcgcggtt tctccgtcag
     1501 tatggatcct ttcatccaga tagggttgcc gggcacgttg tgcaatgtgg acattttccc
     1561 gtatgacacg gcccctgccg cgtggtctcc ggatgcccct gaggaagagg agtggctgct
     1621 gcgcggttac aaggcatccg gaatgcttga ttatgaggcg gattcctcct gccgctaccg
     1681 gactcggtgt tcctatgagg aaaagatggc gatccgggac cgggtggtga tgggaggacg
     1741 gcggccggcg gatggaggaa acattttcct gggttctgag attccttttg ccggcccgtg
     1801 ccgccattcc ttccgccatg aatggctgtt tccgctttcc cgcgtccggt ttgagggaag
     1861 ggagttcccg gccccgcggt tgcctgaaat ggtgttgtac ggccagtacg gggactgggg
     1921 agctctgccg gattctcccc ctgtgcattt tgatttgaac cacatttcca aggaggtgct
     1981 ggccaggatg atgtacatgc gtgatacggg gcgcctcccc gggcgcgatt aaaaggccgc
     2041 gcctcttttt tcccccgcag ggagcagacc ttctttcttc cttgaccctg ctgactgagg
     2101 aggagtatca tcagatgatg agaagacccc cgattcccgg cttggtgatg gcggacggat
     2161 ggctccagcc gtattcccgc cagatacgcg accgccagcg tctgtttgac ctgaaaatga
     2221 agaggatcca tcagcgcgcc ggctctctgg aggaatatgc gcggggatac cgctattacg
     2281 gtttcaacag agacgcggag acgggggcgt ggacgtaccg ggaatgggct cccgccgccc
     2341 gcagggtgtc cctgattgga gattttaacg gctggaaccg ggagagccac ccgctggagc
     2401 ggaatgagcg cggcgtgtgg gagattacgc tgccgcctga tgcgctggcc cacggacaga
     2461 aggtgaaggt gcacgtggtc ggtgcggacg gcacgggcag agatcgcatt cccgcgtgga
     2521 ttaccagggc cgtccaggat ccgtccactt atgatttcgc cggggagatc tggatgccgg
     2581 aacaccccta tgaatggtgg aataacggtt ttgacccctc ccgggtggaa gttccgttcg
     2641 tgtatgaagc gcatgtaggc atgggcgggg aagaagggcg cgtgcatacg taccgcgagt
     2701 ttgcggacga ggttctcccc cggatttcca ggctgggtta caataccgtc cagctgatgg
     2761 cgattcagga gcatccctat tacggttctt tcggctacca tgtttcttcc tttttcgccc
     2821 cttcctcccg tttcggcgag ccggaggacc tgaagtacct gatagaccag gctcacggcc
     2881 tgggcatcgc cgtgctgctg gacgtggtgc attcccacgc cgtgaagaat gaggcggaag
     2941 ggctgaacaa ttttgacggt tccggaggca tgtatttcct gcccggggag cgtggccgtc
     3001 atccggactg ggattcctgt tgttttgatt atggccggga cgaggtgctc gagttcctcc
     3061 tgtccaatgt ccgctggtgg ctggaagagt tccgttttga cgggttccgt tttgacggcg
     3121 tgacttccat gctgtatttc caccgtgggc atgagccgtt cggggatttg ggcgcctact
     3181 tcggctcttc cgtggatctg gatgccgtgg cttatctgca gctggccact acgctgattc
     3241 agcgggtgaa gccgggcgcc atagcgattg cggaggacat gtccggcatg ccggggttgt
     3301 gccgcccggt ggacgaaggg gggattggct tttcccaccg tctggccatg ggcattcccg
     3361 attactggat taagttgctc aaggagaaaa aggatgagga atggagcatg ggcgacatgt
     3421 ggcacacgct gaccaacagg cgctacggag agccgcatgt ggcctattgc gagagccatg
     3481 accaggccct ggtgggagac aaaactctgg cgttccgcct gatggatgcg gaaatgtact
     3541 ggaaaatggc cgtggaccag cagagcctca ttattgaccg cggcatggcg ctgcacaaga
     3601 tgatccgcct ggtgacgctg gctaccgggg gggaaggctg gctgaatttc atgggcaacg
     3661 aattcgggca tccggaatgg attgattttc cgcgcgaagg caacgactgg tcttacgaat
     3721 actgccgccg ccagtggtcc ctggtggaca atccttccct caggttcaag ttcctgaatg
     3781 cctttgacca ggcgatggtc cgcctggcgc aggaagcccg gctgctgaat aatccgccgc
     3841 ctttcccgct taatattgac gagaccaacc atgtcatggc attccaccgc ggcggcctgg
     3901 tatttgtgtt caactggtcc ggagacaggg ctatcatgga ttacatgctg cctgttcccc
     3961 agaaggggga atggcgggtc gtgctggata cggacaacgc ccgtttcggc ggtttcggga
     4021 ggcaggatgt ttccatgccg cattttacgg atggggacgg gaacctttcc ctgtacctgc
     4081 tgccgcgtac ggccctggtc ctgaaaaggg taggttccgc cgtcatggcc cgccgcctgg
     4141 ggcgggagga ttagcgtgcg gcctcaaaaa aactggcggg tttattggat ccgtccatga
     4201 aagaggcgtg cggtacgggc ctgcgtaccg ggcgcgggat gaataggcgc tttccctgcc
     4261 ggaacgtgtc cggcaggctg ttaccactgc gcgggaagtt cccgcaaggg gggcactcct
     4321 gaatccgcct ttttcaggcg gatggtcagg atgtccgttt tgaagtcctt ggacttctcg
     4381 ttattgcggc cccacaaaat ggctccttgc gtggtcattt ccggataaaa cgttttttcc
     4441 gtggaaagga agcccggttt gtccgccgca acgtgcacgg gcttgtgcct gttcagggag
     4501 agcgtgacgg gggcctggcc ggcgtagtct ccgttcacgc gcaggctggc tccggaagga
     4561 atgctgcgga tggtgatgtc ccgggaagag ctgctgcatg agaggcttgc gccgcagaat
     4621 gcggagagca ggagaagatg cgggaaaagg ccgtgagtca ttgctttgga aaaatcaatg
     4681 gtgttccgcc atttttatgg cggcctgctt gatctgggcc gccatggagt tgagggcgtt
     4741 cgccctggtg ggcgccaggt ggtctttcag gccggttttg tccagcttgg acatgtccgc
     4801 cttcaggatt tcttccggag gcaggccgga aagcagccgg atgaagacgg caatgaggcc
     4861 cttggtgatg agggaatcgc tgtctgcgta caatttcagt acgccgttgt ccggttccgt
     4921 gtgcagccat acgcgggatt ggcatccctt gatgagggag tcctccgttt tgtaggcttc
     4981 atccagcctg ggcagttttt tacccaggct gatgatgtat tcgtagcgtt ccgtccagtc
     5041 ctggaacaaa tccagttcat ccagcaggtc ttgcaggcgt tcttcgtagt tcatggctcg
     5101 gagattgggt aggatgagcc gggcgggtta tccccggcgg gcgtccgcga gcgtggcgag
     5161 cacggctttc tgggcatgca ccctgttttc cgcttcccgg aagataacgg gagcaaaatg
     5221 ttccagcacg tcttccgtga tttccttgcc gcggtaggcg ggcaggcagt ggaaaacgga
     5281 gtggccggag gcggcatgtt ccagcagctc cctgttcacc tggtagggat agaagtgttt
     5341 ttccttggac agtccttccg attcctggcc catggaaagc cagacgtccg tgttgatgac
     5401 ggaagctccc ttgacggctt ccacgggatc cgtggtgaag atgacgttct cgcagtccag
     5461 atgcctgcgg aagtcttcca ggggaaggta attggtaggg gctccgatgg cgagcgtaaa
     5521 gcccagcctc ttggccgccc acatccagga gcgggacatg ttgttgtccc cgtcccccac
     5581 gaaggcgatt ttcatatcct tccaggaacc ggggccgtag atttcctcaa tggtaagcag
     5641 gtccgccaga atctggcagg ggtgctcctc gtccgtcagg gcattgatgg tgggaatgcc
     5701 ggaaaagctg gcgaattcct ccacttcctg ctgtccgtaa gtccggatgg cggcaccgtg
     5761 gatcatacgt cccagcacgc gggccgtatc cttgatgggc tctccacgcc cgagctggat
     5821 gtcatggacg gaaaggaaca tgggacggcc ccccagttca ctgatgccca cttcaaagga
     5881 aacgcgggtg cgggtggagg atttggaaaa gatgagcgcc caggtctgcc ccttcagggg
     5941 aaggcgttcg tgatggccgc gttccgcctt aagccggtgg cccagggcga gcaggtcccg
     6001 gatttcgtct ccggtaagct gttcgatgga aaggaggttg ttcatggttt ttgaaaggga
     6061 ataaaaacgg gaaagccgca ccatactccc tgttttctta aaagaaaagg gaataatgcg
     6121 gctttccagg gagggatgga gatgccttta cagcctggcc gccgcctggt ccggccacgg
     6181 tacgggagcg ccttcacggg ctccggtggg attattcttt ttgtaggtgg gcatttgggc
     6241 cttgcgctcc ttgagcaggg cggtcatggt cctggccatg gccttaacgc gttccggctg
     6301 ctgttccgcc aggttgtgct cctcgctgat gtcctccttg aggttgaaga gttcgaactt
     6361 ctggtcaggg tgccagtaga taagcttcca gtccccctgg cgcatggccg tgctggggga
     6421 ataacgggag ttgtttccgt tcccttcccc ccatacgttg ggcgtgtgga agagcagggg
     6481 gcggttggga ttcatgctgc cgcctttcag cagggcaaca aagcttttgc cgtccacaac
     6541 ctgaggcgcc tgaatttttt ttgctcccgc tatttccaga atggtgggga aaaagtcttc
     6601 aatgatgacg ggagtggcgc agacgctttc cgcctttgtg accccgggcc agtagacgat
     6661 catcggttcc cggatgccgc cttcccggtt ggaacccttg ccgaaactaa gcgggtaatt
     6721 ggattccttg ttgcccatgc gtccgctgat ggcgaggccc ccgttgtctg ccatgaagat
     6781 gatgacggta tttttatcca gattgttttt tttcaggtat tcccggatgt cgcccaggct
     6841 cttgtccatc ccctggatca gggcggaata gcgtttttcg ttgtcggacc atttatggcc
     6901 gtcattgggg ttggagtact cttccgcaaa cctcttgtca tagccgcgta tgtcgaaggg
     6961 ggcatggatg gcgtaatggg acatgtacag gtagaaaggc ttgtccgcct ccctggggtt
     7021 tttccgaatg gcgtccaggc gtttcagcgc ctcttgcgtc agggcttccg tcaggaatgt
     7081 gtcgttttca tagtaattat tttcatccag cccgcggaca tggaaattcc ccgtgccgta
     7141 tttccgggaa ccccggtagt ccgccgggcc gcctatttcc gtgccggcga tattgtagtc
     7201 aaagccgaag agcctgggat tggctccggg cgtatttttg gagccgaaat gggcttttcc
     7261 gcagtggatg gtggtataac cctgtttttt gagcagggcg ggcagcccaa gcacctgggt
     7321 gaagggtttt tccatcctgt actggatttt ttcttccgtc aggggaagat gggtggttcc
     7381 ggatgccttg gtccctgccg gctggatgcc gttgacgctc cagtctgcgg gggccttgag
     7441 cgctttgtgg cctgcgtcca gttcgtgacg cggtggcggg cggagttcat gccggacatg
     7501 aggctgcatc ggctggggga acagataggc tgcgcgtagg cgttcgtgaa gaccatgccc
     7561 tgcttgccca gcgcctccat gttgggcgtg cggtagcgct tgttcaggaa agtaggtttg
     7621 ggagtgccgt cctcttcccg ccaaaagggc agggatgtat cctgccagcc catgtcgtcc
     7681 accagaaaga ggatgatgtt ggggcgtttc ggttctgccg cgcgggatgc ttttacggaa
     7741 gctgcggaag cggagcaggt gcttcccagc aggaacggga tgagaaaggc tgcgatgcgg
     7801 gaaatgttca ttactaaaaa tgaccggtac ttataataac gcatgagacg gacgtttctg
     7861 tcaaggattc gtattcccgc ggcagtttgt tcggagacgt cctgttttgc cggtgcggcg
     7921 tgtaccggga tggaagagcc ttattcaaac agggccgggt aggatttccg ggccagggcc
     7981 gtgctcaagt ccacgatagc cctgctgttg ggggcctgga gcgccttttg ggccatgtcc
     8041 cggcactggc cgtagtccag attgcggatg gcataccgga tgatggggac aaggtggacg
     8101 cccacggaca tggaggtggc ccccaggccg atcagcaggg gaagcagggt aatgtccccc
     8161 gccatttccc cacagatggc cgtgggaatg ttttcccgtt ctccggcggt gatcgtcata
     8221 tccatgatgc gtatcacggc aggatgcgtg gggcggaaca tgttggcgac gcggttgttg
     8281 acccggtcca cggcaatagt gtactgggtg aggtcattcg tgcctatgga gaagaaatcc
     8341 acctcacgcg ccagcacgtc cgtcatgatg gcggcgctgg gcacttcaat catgatgccc
     8401 actttcaagt cccgcgcata ggggatgttc ttcctttcca gttcttcccg gcattcctgg
     8461 aggatgtgct tggctgtgac cacttccgta tagccggaga ccatggggaa catgatgccg
     8521 cagccgggcg tgtccgcgca ggcacgtaga atggcccgaa gctgctgctt gaacagctcc
     8581 gggcggctga gagagacgcg gatgcctcgc cagccaagga aggggttcgg ttccggagtg
     8641 cggagctgtt cgcacggcag cttgtcgccg ccggagtcca gggtgcggaa gatgacgccg
     8701 tggggggagc aaccttccgc cagctttctg tagtagcgca tttgttcttc ttcatccggc
     8761 atgccggatg ggttgctcag cagaaagaat tccgtgcgga acaggcctac cccttccgct
     8821 cccacctcct tgatgccgga atattcatgg gggaactcca cgttgacgga caggcggatg
     8881 ttgcgtccgt ccctggtgat ggtgggcagg tcgcgcagcg cttccagcgc cttgtaggcc
     8941 ttttcctttt ccacttggag gcggtggtag tattcctccg tttctttcgt gggtttcagg
     9001 atgagcacgc ccttgtagcc gtccagaatg gcgggtgagt ggctttccac tctcagaagg
     9061 gcctggtcaa gccccacgac ggcggggatg cccatggagc gggcgagaat ggctgtgtgg
     9121 gaaacggaag agcctatttc cgtggcaaag ccgtgaatca ggctggcatc catggcggcc
     9181 gtgtcggaag gagtcaggtc gtaggccacc agaacctggt ctttttccgt ttcttcctcg
     9241 tcttcaggag gttctgtgct gcggaggttg ttgatgaccc gctgcatgac atcctccatg
     9301 tccgccgttt tggcgcgcag gtaggggtcg tccacacggc gcagaacttc catgtagttc
     9361 tgcatgacca cataaaagat gtgctccacg ttctggaggc gcttgagcag ttccgtctga
     9421 acctgctgga ggataatttt gtcctgaagg agaaggatgt gggcgtcgaa gatggaggca
     9481 tctttctctc cggatatctg gatcatgcgt tcccgcagcg tcctgatctg gtccgcagtg
     9541 gtttgcagcg cccggtggaa gcgcaccagc tcagcatcca cttcttccgg ggaaatatcg
     9601 tacgctacgg gttccttgga accgctgatt tgaattttaa ttttgcccag ggcaatgccg
     9661 ggggaaacgg gaatgccctg aagccacgtt tcttccgttg aaatgggttc ctgaatcatc
     9721 tgcgggctta atggcccggt ctcatatggc cgtgagccgg agtgttcaat cttcttcaaa
     9781 cttgcgggaa atcaggtctt ccagcgcatt cagcgcttcc tgttcgtcct tcccttccgc
     9841 cgtgatggtg atcttggagc cgtggcctac cgccagcatc atcaaaccca gaatgctctt
     9901 gccgtcaact tcctcgccat ccttctccac gacaatgtct gcatcaaatt tgctggccag
     9961 tttgacgaat tgagcagccg ggcgtgcgtg aatgccgagt ttattgatga tggtgagttc
    10021 cttggtgacc atggaatttg aagtaagggg tgataacggt tgctacaacg gtaagtacac
    10081 taattaaagt ttttttccaa gagttttttg tcagtcctag ttttcggagg attggaagtt
    10141 tgacatgagg cggcggttga actccccggc catgtcgatg cccatgtcct taagatgata
    10201 gtccatggcg gctacttcca ccagcctggc aatatccctg ccgggagcca cggaaagctg
    10261 aacatggagc actttttccc ccaggacgtc cagcccctcc ctctccaggc caagtctgtc
    10321 cagctcttcc tggtccttgg cgggaatgag ggtgacgatg agatccagac gcttgttgtg
    10381 gcggatggat ttcaggccga aaagggtggt gatgttgacg atgcccaggc cgcgcacctc
    10441 cataaagccg cggctcattt ccggggcgct ggccaccagt tctccgccca cgttgcgtac
    10501 gtacaccatg tcgtccgcta cgagggccgc gccgcgttca atcaggccca gggccgtttc
    10561 actcttcccc acgccgctct tgccgcggat gaggacgccc actccgcgca cgtccaccat
    10621 gcagccgtgc agggtgacgc tttccgcgaa ttcgttttcc agaatgatgg tggcggagtt
    10681 gacgaatttc atggtcacca ggctcgtgcg gaagacgcag acgccggctt catccgccag
    10741 ctccacgatt tcccggggtg gattctggtc acgggagaag acgatgcccg gcacctcgca
    10801 ccggaacatg cgctgaatgc gcgatttgcg catgccttcc ggcagttttt gaagataggc
    10861 cagctccgcg gagccgaaaa cctggatacg tttgttggca aagtaggagt agaagccggc
    10921 gatcgccagc ccggggcggt tgagggcggg ttcgcagata tggcggctga ggccggcggg
    10981 gctgttgata agttccagtt gaagggctt
//
LOCUS       DAOMSU010000051        10894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_353_length_10894_cov_3.395885, whole genome shotgun sequence.
ACCESSION   DAOMSU010000051 DAOMSU010000000
VERSION     DAOMSU010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10894
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_353_length_10894_cov_3.395885"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2819)
                     /locus_tag="PK077_03645"
     CDS             complement(<1..2819)
                     /locus_tag="PK077_03645"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HRN23422.1"
                     /translation="MRLILPLPLRRFLLGRLLFSVTMATAASGAEFVVDAAQAANPGQ
                     HVYNTLQELADSGALSSGDTVILHNDDSSLTAGLPVAVNFRSNDPGIFRTIDLAGLGE
                     REVLYDFSGAGLNDITLEMNSIILSGSVNQLTRSMTFSSLMIEGKVQFDRNCVENRRD
                     NVDRIAIALLYSSHRGEILKLGDGVIFSNNCVLGVDNRSARGGVLLNSSISPITIGDH
                     AVFSGNYAFSNLNRAEGGVIYSNDNTITVGDNSFFIGNYASSVSKSAIGGVITSNKAK
                     STVGANATFIGNYAFSNLNGAFGGVFYSARHRSVGMGFHGEIAVGANATFINNYAFSN
                     STTASGGVICSSGDISFSDGATFINNYAKTSGGAIELAGKLDLLALTKDVLFSGNMTG
                     GVFTRHDDGTFSVENGVANAIHLTTGATIPESAIETQGSLALAAEEGREIRFNDPITS
                     RSENSDCPMTLTLNRCTDEEGNIHDTDGTIIFSSELYQGEEAHLVASRYNNFVADTTL
                     YGGTLLLEHGAVFGRNLEDMRNLGNDSSSLTVEKGTLEIIGGSTANAAAFSLSGRDAV
                     LRPGDSAFINANTADFSRGFTFDMRHQLQSGPGSGPGLTLSAAQSFTAGGLIGVADTG
                     SNAPYFYADRSWKQDRVFHVLTDAEHTLQGNFDGVVSQATGTDRVDSPYSYTGTWSHR
                     WTDADGDGYAEQLQLVWKADGTPISSIDPELVGELAFNSLWSSASNAAALGGNVLSRL
                     NVFRLADKHARDLWGMGLGDFARQRSRGGADGYDYSGGGYSVGADSGFGHDNGIWGIA
                     FGQLYGHAKSRGFQGRNTQDTRMGSLYWGRLLEESRRARWTFKGSLTWAETHNKMTSR
                     LSGAPASTGKWNNETWLAQAEVSRTADYAGGWRLTPFLRVEFTHGRQDAFREQGGYGR
                     DFGGAALKRLSIPVGLEIGRTDEWKG"
     gene            complement(3000..6099)
                     /locus_tag="PK077_03650"
                     /pseudo
     CDS             complement(3000..6099)
                     /locus_tag="PK077_03650"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
     gene            complement(6106..7260)
                     /locus_tag="PK077_03655"
     CDS             complement(6106..7260)
                     /locus_tag="PK077_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRN23423.1"
                     /translation="MKIINLLFCMPLLVVCFSCQREGESQAGGLPGEIVPEVSVMTVR
                     STQVPVTANLPGRMEAYLQAEVRARVTGIIQERCYQEGQTVRPGDLLFKIDPAPLQAV
                     LDECKAAVARARAVLSDAEDKAARYSSLVAKGAVSIREHKQARAEEERARAEYAAAAA
                     SLEQARLNLEYTRVEAPISGRVRRALVTEGAFANQNEFTHLTTIEQIDPIYVRFSQPA
                     SQYSSLRRAVVSGLWKGVPLGEIKVRLLLSNGEEYPHSGRIIFSDMAVDPNTDTIEMR
                     ALFPNPDHELLPGAYVRVVFDRAVRDNVFAIPRDAVIRTAQGASVFVVGPEGVLEARP
                     VRADTLNGREWLVSEGLRDGDRVAVSHTMSLRPGMKVRSAAARPQPHAQQ"
     gene            7557..8369
                     /locus_tag="PK077_03660"
     CDS             7557..8369
                     /locus_tag="PK077_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRN23424.1"
                     /translation="MEKQNLLTIGNLSKQTGVHVKSLRYYEQLGILLPAYTDPDTGYR
                     YYTLSQIPVVHAICACIFLDIPLKEFTGFLTRDQRRIHYKKLFSHGTMLAHRKIRDIQ
                     EKLRLLEKFRKQMGREEDLLLHGEQAIHALPEKYCWVAPYAGKQHTPEFNILITRIFD
                     DINRCQLRLGAEAGLLSFHRKAGTEQFLYVEVEAAGRDAQRMKKIMRLPAASFLCAIR
                     QESGIAEAPGVFPDLFAQEYDKIIMETELFPGDYDVANPKFELRCSLPPGGK"
     gene            8554..9552
                     /locus_tag="PK077_03665"
     CDS             8554..9552
                     /locus_tag="PK077_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRN23425.1"
                     /translation="MAHCDTTQPDLKENAVRAFKAMLLARAFDTKISSLYKAGKITGG
                     VYLGRGHEAIAACGGVFLTAGYDVIAPFIREQAARVTWGEPIIEAARAYLGSALGYMK
                     GRDGNVHRGLPAEGYMAPISHLGSTVAFVIGCLFAKRLDGKLPGPVGVAFCGDGTTST
                     GAFHEAANMANVERLPLVLVVTNNQFAYSTPNIREFGEASLADRGRGYGFTVHETDGT
                     DFMATLETFRTAVNNAREGRGPQWVLAKTLRMCGHGEHDDASYIPRELKEEYEKKDPV
                     AVAERQLLAAGWLTPEETALKKQYADEVQLAVATAQREPEPDPFREDWNATVWRPY"
     gene            9574..10548
                     /locus_tag="PK077_03670"
     CDS             9574..10548
                     /locus_tag="PK077_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRN23426.1"
                     /translation="MSVTYIDAIHDAQKDLLTEDRDVFLYGQDIGVFGGAFKATKGLK
                     ELFPDRVIDAPISEDAMAGMVTGAAVMGKKPIMEVQFADFSTIAFNQIVNMAATHYYR
                     TGIPANITVRLPCGGTPGTGPFHSQSLEALFAHYPGLHVMTPATVADAYWMLRQAVEI
                     PDPVIFLEHKFLYRWLKAEDNYREAPVIPFGTARIARTGKHATVVAYSAMVPEAVRAA
                     DLLEKESGYEVEVVDLRTVRPLDMDTVIASVARTGRVLVVGEDFPWGGVTAEVVSRIV
                     AEGFHLLDAPPQRLNARDTPIPQHPNLWKAHRPTLESIAASIRHLLSI"
     gene            10567..>10894
                     /locus_tag="PK077_03675"
     CDS             10567..>10894
                     /locus_tag="PK077_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HRN23427.1"
                     /translation="MPKVPILMPQLGDSIAEATVLRLLAAQGDTVEADQEIFEVETNK
                     ATMGVTTMCGGILSDVFIKEGESVVVGACMAMIEATEEEIERSGATPAGDSTQPSLPA
                     SPESVPQ"
BASE COUNT         2330 a         3241 c         3195 g         2128 t
ORIGIN      
        1 cccttccatt cgtccgtcct gcctatttcc agccccacgg gaatggacag gcgcttcagc
       61 gccgcccctc cgaaatcacg cccataccct ccctgttccc ggaaggcgtc ctgccggcca
      121 tgcgtgaatt ccaccctcag gaagggcgtc agcctccatc ccccggcata gtccgccgtc
      181 cgggacactt ccgcctgcgc cagccacgtc tcattgttcc atttgcccgt agaagccgga
      241 gctccgctta ggcggctggt cattttgttg tgtgtctccg cccacgtgag gcttcccttg
      301 aaggtccagc gggcgcgcct gctttcttcc agcagacggc cccagtacag ggagcccatc
      361 cgggtgtcct gcgtgttccg gccctggaag ccccggcttt tggcatgccc gtagagctgg
      421 ccgaaggcga tcccccagat gccgttgtca tggccgaacc cgctgtccgc ccccacggag
      481 tagccgcctc cgctgtagtc gtagccgtcc gcgcctcccc ggctgcgctg cctggcgaag
      541 tctcccaggc ccatgcccca caggtcgcgg gcatgcttgt cggccaggcg gaagacgttc
      601 aggcgggaaa ggacattgcc gcccagggcc gccgcgttgg aggctgagct ccagagggag
      661 ttgaaggcca gttccccaac cagttccggg tcaatgctgc tgatgggggt tccgtccgcc
      721 ttccagacca gctggagctg ttcggcgtag ccgtctccgt cggcgtccgt ccaccggtgg
      781 ctccaggttc ccgtgtagga gtagggggag tccacccggt ccgtccccgt ggcctgggag
      841 acgactccgt caaagtttcc ctgaagggtg tgttccgcgt ccgtcagcac gtggaagacg
      901 cggtcctgtt tccaggagcg gtccgcatag aagtacgggg cgttggagcc cgtgtccgcc
      961 acgccgatga ggcctccggc cgtgaaggat tgagccgcgg agagcgtcag gcccggcccg
     1021 gagcccggcc cggactggag ctggtggcgc atgtcgaagg tgaagccgcg ggagaagtcg
     1081 gcggtgttgg cgttgatgaa ggcgctgtca cccgggcgca gcacggcgtc cctgccggag
     1141 agggagaagg cggcggcgtt ggccgtgctt cctccgatga tttccagcgt acctttttcc
     1201 accgtcaggg aagaagagtc attacccaga ttccgcatgt cttcaagatt cctgccgaag
     1261 acggcgccgt gttccagcag gagggttcct ccatagaggg tggtgtcggc cacgaagttg
     1321 ttgtaccggc tggcaacgag gtgggcttct tccccctggt agagttcgct gctgaagatg
     1381 atggttccgt ccgtgtcgtg aatgttccct tcctcatccg tgcagcggtt gagcgtgagg
     1441 gtcatggggc agtcggagtt ttcgctccgg ctggtaatcg ggtcgttgaa gcggatttcg
     1501 cgcccctcct ccgcggctaa ggcgagactg ccctgtgtct ctatagcact ttcggggatt
     1561 gttgcacccg tagtaaggtg aatagcattg gcgacgccgt tttccacaga gaaggtgccg
     1621 tcgtcatggc gggtgaagac tcccccggtc atgttgccgc tgaaaagcac gtctttcgtc
     1681 agggcgagaa ggtcgagttt ccctgctaat tcaatggctc ctcctgaggt cttggcatag
     1741 ttgttgatga atgttgcccc atcgctgaaa gagatatccc cactagagca aatcacgccc
     1801 cccgaagctg tagtggaatt gctgaaagca taattgttga tgaatgtagc attggctcct
     1861 acagctattt ctccatgaaa tcccatgcca acagagcgat ggcgcgcgga gtagaatacg
     1921 ccgccaaaag ctccattcaa attactgaaa gcataattgc cgataaaggt tgcattggct
     1981 cccacagtag attttgcttt gttggaggta ataactcctc cgatagctga cttggaaacg
     2041 ctggaagcat aattgccgat aaagaaggaa ttgtctccta cggttattgt attatcgtta
     2101 ctataaataa cccctccttc agctctattc aaattactga aagcataatt gccagagaag
     2161 acagcatgat ctcctatggt gatcggcgaa attgaactgt ttaacaatac cccgccacgg
     2221 gccgacctgt tatcaacgcc tagcacacag ttgttggaaa aaataacgcc gtctcctaac
     2281 ttcaaaatct ctccacgatg agaactatat aacagagcaa tggcaattct atcgacatta
     2341 tctcttctgt tttctacgca gttacggtca aattgtacct taccttctat cattaacgaa
     2401 gaaaaggtca tactccgagt tagctggttt acggaaccac tcaggatgat ggaattcatt
     2461 tccaatgtta tgtcatttaa ccctgccccg gaaaaatcat aaaggacctc cctttctccc
     2521 aatcctgcca aatcaatggt acggaaaata cccggatcat tggaacggaa attgacggct
     2581 accggcaagc cggcggtcag cgaggaatcg tcattatgca gaatgacggt atccccggaa
     2641 gacagcgctc cggaatcagc cagttcctga agcgtgttat acacatgctg ccccgggttg
     2701 gctgcctggg cggcgtccac cacaaattcc gcgccggaag cggcagtcgc catcgttacg
     2761 gaaaacagaa gacgccccag caggaagcgg cgcaacggca atggaagaat aagtctcata
     2821 tcttttaaaa agaatcatta atatcggaag gcccgctaaa tagcggattc gaaatacgcc
     2881 cgtcaataac cctcgtgcac ggcatgcggg aactcctacc gcaacaaggc ggcaggagcc
     2941 tttcacggcc cctgccatgg ggaaaacaag cgtgctccgc ccccctattc cggcggcgtt
     3001 cacgaaaagt ttcccgcggc gggaatccgg ccctgggcct cttccggatg agccgggttc
     3061 ctctttccgg ccgtcaggcg gaagaagagg ggcaccagga agatggccag cagcgtggcc
     3121 gtaatgatgc cgcccagcac gccggtgccc acggcgcgct gcgctccggc cgccgcccca
     3181 tgggcgaacg ccagaggaat gacgcccgcc ccgaacgcca gggaggtcat caggatgggc
     3241 cgcaggcgca gtttggcggc ggcgacggca gcttccgccg cattcatgcc ctcccggtga
     3301 aattccctgg ccacttccac aatcagaatg gcattcttgg cggaaaggcc gatggtcgta
     3361 atcaggccca ctttgaagta gatgtcattg ggcatgtcgc gcaggaagac ggccagcagg
     3421 gctccaagca gtcccagggg cacgaccaga ataacggcca acggaatgga ccagctttca
     3481 tacagggccg ccagcaccag aaagacaatc aggatggaaa ggccgaaaag cagcgtggcc
     3541 tgggacccgg ccagcttttc ttcataggat tgccccgtcc attcaaaccc cacgccctgc
     3601 ggcagggagg cggtaatctg ttccagggcg ttcatggcat ctccgctgct cttgccgggc
     3661 gcggaagagc cgttgatggt gaaggaggga tagttattgt agcgcgtgag ctgcggaggc
     3721 cccgccgtcc agtccagcct gacaaaggag gaaaggggca gaagcttccc ctggtcattg
     3781 cggacatgca ggtcctcaat gtcgtttccg ttcaggcggc tttttccgtc cgcctgcacc
     3841 atgatgcggc gcacctggct gtcatgcatg aaatccccca cgtagctgga cccgaacatg
     3901 accgccagag aattgctgat ctccgcaatg ggaactccca tggaaaaggc tttttcccgg
     3961 tcaatggaga cgtgcagacg cggcgtatcc gcctggccgg agaaataaac gcccgtcagg
     4021 ttggggttcg cgttggcacg ggccaggagc tcgtcccggg cttccgccat gcgggcatat
     4081 cccaggccgc ccttgtcctg gaggcggaag tcgaatccgg acgaattgcc cagctccggc
     4141 agagccggca tgttcagagc catgaccatc atctggccgt ctccggcaaa gcgggcattg
     4201 acgcggtcaa taatcgcctg gacagaactg ttttctccgg gacgctcgtc ccagtttttc
     4261 agaccgacaa acagcatggc catgttggtt ccggagccca tgaagctgaa ccctcccacg
     4321 gagtaaacgt gctccaccgg ctcatgccgc atcaggtaat cctccacttc ccgcagccgc
     4381 acgctggttt ccttctgaag ggtgccggca gggagggaca ccatcgccat gaagttgccc
     4441 tggtcttctt ccggaaggaa ggaggtgggc agcgtaaggt acaaatagcc ggcccctgca
     4501 ataatgaggg catacaggaa cagggagcgc acagggcggc ggatcattcc ggatacggca
     4561 cggccgtacc ggtccgtgga ccggtgtatc gtccggttga accatccgaa aaaacccttt
     4621 ttctcctggt gctccacgga ggagggtttc agcatggcgg cgcacagcgc cggagtcagg
     4681 gaaagggcca gaaacgcgga gaaggatatg gacacaatga gcgttacggc aaactgccgg
     4741 taaatgttcc ccacagctcc gctgaaaaag gccatgggca cgaaaaccgc cacgagcacg
     4801 gcggtgatgc ccacgatggc cccgccgatc tgcttcatgg ctttcaccgt agcgcgccgg
     4861 gcatccaggc cttcctccat catgatgcgt tccacatttt ccaccaccac gatggcgtca
     4921 tccaccagaa tgccgatgct gagcaccatg ccgaacatgg taagcatgtt gatggagaac
     4981 ccggagagcc acatcacggc aaaggtcccc agcagggcta cggggacgac gagcgtggga
     5041 atcagggtag cccggaagtt ctggaggaac aggaacatga ccaggaacac cagcaggacg
     5101 gcttccagca gggttgaaat gactttccgg atggagattt ccacgaagtg ggtggtttca
     5161 tagggaatct ggtaggagac tccgggcggg aaatccctgg acagttcatc catcttggcc
     5221 ttgaggaggc ccatggtttc cacggcattg gagccgggag ccatcttgat ggccattccg
     5281 gtggccgtct ggccgttgca gcgggaaaag aacgcgtagc tgttggcgcc cagctccacc
     5341 ctcgccacgt cccccagttt gacggaggaa ccgtctgaaa gcgtcctcaa cgcaatggag
     5401 gcgaattcct ccggcgtacg gaaagcgtcc tccgccacca cgccggcgtt gatgggggcg
     5461 gattcgggaa cggccgcccc cccgatgtcc ccgatgatga tgcgctcgct ctgggcagcc
     5521 acggcattga caatatccgt gggggtcagg cccagagcct ccagtttttc cgggtccggc
     5581 cagatgcgca tggatgtctc cgcaccgaaa agctccacac gcccgacgcc tttcacacgc
     5641 ttcagttccg ggatgatgcg ggcggaagcc cattccccca gctccgtatc attcaggctg
     5701 cctgtagaag agagggagat catcgcctga atattgttgg atgatttttc cacgaacacg
     5761 ccttctctcc ggacggattc cggcaggcgg gattccacga ttttcagcct gttctgcact
     5821 tctacggcgg caatatccgg atccgtgccc tgcgtgaacg tagcggctat ttccacggtt
     5881 ccgtcggagc tgctggtggc ggacattgac ggcagtcacg gcttcctccg tatcctgggc
     5941 ggaagccccc ggatatgtgg tgcgcagggt gatgaccggg ggagcaatgt ccggatactg
     6001 ggcaatgggc aggctgggga tggccagaag gcccatcagg gaaatcagga gtgcgactac
     6061 ccaggcaaaa atggggcggc gtatgaagaa atcaggcatg gcggtttatt gctgggcatg
     6121 gggttgcggc cgggcggcgg cgctgcggac tttcatgccg gggcggaggg acatcgtgtg
     6181 gctgacggca accctgtccc catcccggag gccttctgaa accagccatt cccggccgtt
     6241 cagcgtatcc gccctgacgg ggcgcgcttc cagcacacct tcagggccca cgacaaagac
     6301 ggaagcgccc tgcgcggtgc ggatgacggc atcccgggga atggcgaaga cgttatcccg
     6361 caccgccctg tcaaatacca cgcgcacata ggcgccgggc agcagttcat ggtccgggtt
     6421 ggggaacagg gcccgcattt caatagtatc cgtattcggg tccaccgcca tgtcggaaaa
     6481 aatgatcctg ccggaatggg ggtattcctc cccattggag agcagcaggc gcaccttgat
     6541 ttcgcccagc ggcacaccct tccacaatcc ggatacgacg gcgcggcgca ggctgctgta
     6601 ctgggaggcc ggctggctga agcggacata aatggggtca atctgttcaa tggtggtcag
     6661 gtgggtgaat tcattctggt tggcaaacgc cccttccgtc accagggcac ggcgcacgcg
     6721 gccggagatg ggcgcttcca cccgggtata ttccagattc aggcgcgcct gctccagaga
     6781 ggcggcagcg gcggcatatt ctgccctggc ccgctcttcc tccgccctgg cctgcttatg
     6841 ctcacgtatg ctgacggcgc ccttggcgac cagggaggaa taccgggcgg ccttgtcctc
     6901 cgcatccgac agaacggccc tggcacgagc cactgcagcc ttgcattcat ccaggacagc
     6961 ctgaagcgga gccgggtcta ttttaaaaag aagatcgccc ggccggaccg tctgcccttc
     7021 ctgatagcag cgttcctgaa taatacccgt cacgcgggcg cgcacttccg cctggagata
     7081 cgcttccata cggccgggaa gattggcggt tacgggaacc tgagtactgc gtaccgtcat
     7141 gacagacact tccggcacga tttcgccggg caatccgcct gcctggcttt caccctcccg
     7201 ctggcaggag aagcaaacca ccagcagggg catgcagaat aaaagattaa ttattttcat
     7261 caggaatcat gctgtttgga acctcaagaa accccggaac cgcacttccg gcaagaccgg
     7321 ggatttttca attgccatat gatgtaaagt atcccataat gggagagtca agaggcaatg
     7381 gaaaaattaa gtggggaagg tctttgtcat ttattgtaaa ttattgacaa taaaaaccct
     7441 atagatgacg gaaataactt cgtcttcaga aaggtttctt ctgcgcgcag ctcccgtttc
     7501 cgccggggct cttccggcgg aaacaatcaa ttccctccca ccaaccgcaa tacaggatgg
     7561 agaaacagaa tttactaacc atcggcaatc tgtccaaaca gaccggcgta catgtcaaat
     7621 ccctccggta ttatgaacaa ctgggcattt tgctcccggc ctatacggac ccggataccg
     7681 gctaccgtta ctacactctt tcccagattc ccgtggtgca tgccatctgc gcctgcattt
     7741 tcctggatat tcccctgaag gaatttaccg gcttcctgac cagggaccag cgaaggattc
     7801 attataagaa acttttctcc cacggcacca tgctggcgca ccggaaaatc agggatattc
     7861 aggaaaagct gcgcctgctg gaaaaattcc ggaaacagat gggccgggaa gaagatcttc
     7921 tgcttcatgg ggaacaggcc atccacgcat tgccggaaaa atactgctgg gtggcgccct
     7981 atgcgggaaa acagcatact ccggaattta acattcttat cacccgcatc tttgacgata
     8041 ttaaccgctg ccagctgcgg ctgggcgcgg aagccggcct gctttccttc caccggaaag
     8101 cagggacgga gcagttcctg tatgtggagg tggaagcggc cggaagagac gcccagcgga
     8161 tgaagaaaat catgcgtctg cccgccgcct cctttctctg cgccatcagg caggaaagcg
     8221 gcattgcgga ggcgcccggc gtttttccgg acctttttgc gcaggaatat gataaaatca
     8281 ttatggagac ggaattgttt cccggagatt acgatgtggc aaatcccaaa ttcgaactga
     8341 gatgcagcct gccgccgggt gggaaatgaa tccggaaatt tccgcgcatc cgccggcggg
     8401 agcggaagat gcaaaagcgc tcccgccagc cgggaaccgc cccccgcgaa cctccgccgc
     8461 cctgcggagc ggccccgcgc ctgcggacac aaagtctcag gcagtttcat gaaatggcat
     8521 tgcatttcct tatgcagcat ataaaataat ggggtggcac attgcgacac tacacaaccg
     8581 gacttaaagg aaaatgccgt cagggctttc aaggccatgt tgctggcgcg ggcttttgac
     8641 accaaaattt ccagccttta caaagcgggc aaaattacgg ggggcgttta cctgggccgc
     8701 ggacatgaag cgattgccgc atgcggaggc gtctttctga ctgccggcta cgatgtaatc
     8761 gccccgttca tccgggaaca ggcggcacgc gtcacctggg gggaaccgat tattgaagcg
     8821 gcacgcgcct acctgggttc cgccctggga tatatgaaag ggcgggacgg caacgtccac
     8881 cggggcctgc cagcagaagg ctatatggct cccatcagcc atctgggcag cacggtggcc
     8941 ttcgtcatcg gctgcctttt cgccaagcgc ctggacggca aactgcccgg ccccgtggga
     9001 gtagctttct gcggcgacgg caccacgtcc acgggtgctt ttcacgaagc cgccaatatg
     9061 gcgaacgtgg agcggctgcc tctggtgctc gtggtaacca ataatcaatt tgcctactcc
     9121 acccccaata tcagggaatt cggagaagcc tccctggcgg acaggggccg cggctacgga
     9181 ttcaccgtgc atgaaacgga cgggacggac ttcatggcca cgctggaaac cttccggacc
     9241 gccgtcaata acgcgaggga aggccggggc ccccagtggg tgcttgccaa gaccctccgc
     9301 atgtgcgggc acggggaaca tgacgacgcc tcctacatcc ccagggagct gaaagaggaa
     9361 tatgaaaaaa aagaccccgt ggcagtcgcg gaacggcagc tgctggcagc cggctggctc
     9421 acgccggaag aaacggcttt gaaaaaacag tatgccgatg aagtccagct ggccgtagcc
     9481 accgcgcagc gggaaccgga accggacccc ttccgggaag actggaacgc cacggtatgg
     9541 agaccttatt aagccagcaa acggaatatt cccatgagcg taacatacat tgatgccatc
     9601 cacgacgccc agaaagacct gcttacggaa gacagggacg tctttctgta cgggcaggac
     9661 ataggcgttt tcggcggcgc tttcaaagcg accaagggcc tcaaggagct gttcccggac
     9721 cgggtgattg acgcccccat cagtgaggac gccatggccg gcatggtgac cggggccgcc
     9781 gtcatgggca agaagcccat tatggaagtc cagtttgctg acttcagcac gatcgctttc
     9841 aaccagattg tcaacatggc ggccacccat tactaccgca cgggcattcc cgccaacatc
     9901 acggtgcgcc tgccctgcgg gggaaccccc ggcaccggcc ctttccacag ccagagcctg
     9961 gaagccttgt tcgcccatta tccgggcctg cacgtcatga ctccggccac ggtagcggat
    10021 gcttactgga tgctgcgcca ggccgtggaa attccggacc ccgtcatttt tctggagcac
    10081 aagttcctgt accgctggct gaaggcggag gacaattacc gggaagcccc ggtcatcccg
    10141 ttcggcacgg cccggatcgc ccgcacgggg aagcatgcca ccgtggtggc gtacagcgcc
    10201 atggtgccgg aagccgtgcg tgcggcggac ctgcttgaga aggaaagcgg ctatgaagtg
    10261 gaggtcgtgg atcttcggac ggtgcgcccg ctggacatgg ataccgttat cgcctccgta
    10321 gcgcgaaccg gccgcgtcct ggttgtcggg gaagattttc cgtggggagg cgtcacggcg
    10381 gaagttgttt cacgcattgt cgcggaaggc ttccacctgc tggatgcccc gccccagagg
    10441 ctcaacgcac gggatacccc catcccccag caccccaacc tctggaaggc gcaccgcccc
    10501 acgctggaat ccattgccgc ctccatccgc catcttctat ccatttgatt caacccagtt
    10561 tccatcatgc ctaaagtacc cattctgatg ccccagctcg gggattccat tgcggaagcc
    10621 actgtgctgc gcctgctggc ggcccagggc gataccgtag aagccgacca ggaaatcttt
    10681 gaggtagaaa ccaacaaggc caccatgggg gtcaccacca tgtgcggcgg catcctgagc
    10741 gatgtgttca tcaaggaagg ggaatccgtc gtggtcggcg cctgcatggc catgattgaa
    10801 gccacggaag aggaaattga gcgttccgga gcgactccgg ccggagattc cacccagccg
    10861 tcccttccag ccagtccgga atccgttccc cagg
//
LOCUS       DAOMSU010000052        10651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_370_length_10651_cov_2.861363, whole genome shotgun sequence.
ACCESSION   DAOMSU010000052 DAOMSU010000000
VERSION     DAOMSU010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10651
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_370_length_10651_cov_2.861363"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..751
                     /locus_tag="PK077_03680"
     CDS             <1..751
                     /locus_tag="PK077_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HRN23428.1"
                     /translation="RSIVKGNFDVVYDLQESSRTRKRYYPAVRFLLNHDMDWVACNSG
                     ERRKLLKKKSFRWGKVVREPFRLERVLTDLSFMHGEGLHFDELPERYVMMIPGCSPNH
                     PYKRWPVENFCVLAKRLAARGVSSVVIGTRAEAVEVEAIAASSPLAVSFLGKSSLMDI
                     PQMALRSLACVGNDTGPTHMCAYAGVPVTAIFCHRTRNSAITARCISNLVSPGAIEEI
                     TVDQVWSALEPFLPPQEGFEQIRAADFPHGL"
     gene            760..1773
                     /locus_tag="PK077_03685"
     CDS             760..1773
                     /locus_tag="PK077_03685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRN23429.1"
                     /translation="MNISVLVPVYNVEPYLAQCLESICSQTLRELEIVCVDDASTDGS
                     LSILREFAERDPRVKVVQAPENGGLSRSRNLAMSHAVGEYLFLVDSDDWLETDLLEEM
                     YRRAKALDADRLACGFRYYYESAPDREDRFLPEDMAPPEKGWLPCTPETIGKIHHGAG
                     GMMIRRSIVEKHGIRFPEGVACEDLYFHYAVFPWCRRVCVVSRAAYVYRKRAGSITSG
                     FASGSSLQSLDYLTVAELVLKEWKEAGILEEYRTAFLKMLVMGVRNIRKYAPHAVQKE
                     VTRKVADMLRQENLYRPAEDNACLSRREGKLLKAWMGGKSGLDFSYYWKKMRKAGARL
                     LRR"
     gene            1784..2770
                     /locus_tag="PK077_03690"
     CDS             1784..2770
                     /locus_tag="PK077_03690"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HRN23430.1"
                     /translation="MNNPMKKNEFAVVLASDNRGILPLSVTVFSLLNTAGPETFYKIY
                     VLSDGIDGENRASVERLAAPFDCRLEFIDVSGILEEHDFPHTEQWPVPAWGRVFIPEL
                     LKEERGNILYLDIDVLVCRDLTELFRTNMDGKAVGVVFENFSRPGSHFNERLEMPLTC
                     TGYFNSGVLLMNVDVFRERNLVRAVLDYAVTHRDRLTCPDQDALNGALCELTVPLHPR
                     WNWHDGLTRRILKNDPREQFWRGVTPRQAVEAALEPGILHYQGVHKPWRYNWRYEGER
                     YERVMREAGLLRGPLPGRTLPAVLKKHLYRPVYRMTARKILRLKEGFDNRLL"
     gene            complement(2884..3735)
                     /locus_tag="PK077_03695"
     CDS             complement(2884..3735)
                     /locus_tag="PK077_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRN23431.1"
                     /translation="MPFSVKILRFFGILLLSLCALGVAGMTWAYLVEPNLLFVRKVDY
                     RVPQWKGKGTPVKVVIAGDLHLMPTAFDEVRALRYVQRIMRLRPDLILLVGDYARGSS
                     ENASMDPATAARLLKGLTAPCGVFAIQGNHDFTFGWNNWSRELKQAGITVLEDQSSLV
                     TFKDGRQLQLSGILDTTRNVRKRLPKRQSPDIPHFLLSHRPETDRILSHGDVDIVISG
                     HTHGGQVCLPFGLLRFEASPDHALPYTYPWHISGGNTYLITKGLGTSTLPLRFNCPPE
                     IYLLELH"
     gene            3927..4094
                     /locus_tag="PK077_03700"
     CDS             3927..4094
                     /locus_tag="PK077_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23432.1"
                     /translation="MKKITVMALAAFAASIMASCCCQDQGAPPLTPLPKFNDLNQPAV
                     DVAPVVMKSKK"
     gene            4218..5195
                     /locus_tag="PK077_03705"
     CDS             4218..5195
                     /locus_tag="PK077_03705"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HRN23433.1"
                     /translation="MADLIRSHGSFAVIAHVHPDGDAIGSTLALGEALKQMGKKVVMM
                     NEDGVPSNLAFMPGVENIIPTPDEPVDVEVAISVDNGALKRLGERSLEALAGVKVWAN
                     IDHHRTNELFGDVQCVLPDECATGAVLYYFFKYLGVPVTPVMRDALYVAVSTDTGSFQ
                     YQMTTAAVMELAADLIRMGVDVQDINRQLYQEKPWVKMQLMREALNGMKLTPDGRICM
                     FCLTNEAKARIGSRPEDTEGLIDLLRSVQGVWLAAYLEETEDDPRIRISLRSKTPEIS
                     VAELASRFGGGGHSMAAGVRIRGPIEEVRLTILKAMREEVERVLRQKIS"
     gene            5192..5926
                     /gene="truB"
                     /locus_tag="PK077_03710"
     CDS             5192..5926
                     /gene="truB"
                     /locus_tag="PK077_03710"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRN23434.1"
                     /translation="MNVSDSIQVPSGVLLIDKARDMTSHDVVAIARRSLGIKKIGHCG
                     TLDPMATGLLMLVVGKATKLQDKLMCEHKEYAGTLMLGVETSSQDAMGEIVAEYSVDG
                     VTEQAVREAFDRFDGAFEQIPPMVSAIKKGGVPLYKLARKGQEVVREPRPVEVFGHGI
                     SRVAIPEVDFTVQCSKGFYVRSYAYDIGKALGCGAHLSALRRTRSGSFTLDRAITVDT
                     LKTAPREELFRAMLSLKELADILIAG"
     gene            5930..6865
                     /gene="ribF"
                     /locus_tag="PK077_03715"
     CDS             5930..6865
                     /gene="ribF"
                     /locus_tag="PK077_03715"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006747 - FAD biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HRN23435.1"
                     /translation="MVIYREFKGLKSLAAPVHWAMGMFDGVHLGHAGVIAAAVKGAAL
                     DGGIPAVLTFRTHPLARVRPESVPPAIMGEDAEKFALMEELGVKAVLSLEFSSRLASM
                     SPEEFIGELCSSCLVAQIAVGEDWHFGRGRAGNVETLRLLSCRYGFRVTAVPSILQDG
                     ERISSTRIREAVRAADFLQAAGMLGRPYRWKGPVIHGRQLGRLLDYPTANMRPGCGLQ
                     PPHGVYAVRARVAGKKYGGVANLGVRPTVEGETGELLLETHLFGHPGDIYGREMEVEP
                     VRFLRPEIKFPSLEELKSQLARDAAHAAEALEEGE"
     gene            6939..7277
                     /locus_tag="PK077_03720"
     CDS             6939..7277
                     /locus_tag="PK077_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HRN23436.1"
                     /translation="MLVEQLKSKIHRAMITRGDVQYEGSIEIPADLMAAVDLWPGEKV
                     LVASVTSGNRLETYALQGPAGTGNIIMNGGAAHLIKTGERVVIMSFALSDKPIIPKKI
                     VCNEHNEIIS"
     gene            7319..7798
                     /gene="secG"
                     /locus_tag="PK077_03725"
     CDS             7319..7798
                     /gene="secG"
                     /locus_tag="PK077_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRN23437.1"
                     /translation="MNLFLADSLNISIQLLFAVLVIVSLLLLGVVLMQRPKQEGLGAA
                     FGAAITDQAFGARTTDVLKKATVYFGTAFMVLCLGLGMLINRQHVKSSESLLSPEMMK
                     AAARQEASVPAKTPEELQQEELRRRAAEAEAASSQAPAPSVTEAPAPASTETPVPSN"
     gene            7960..8601
                     /locus_tag="PK077_03730"
     CDS             7960..8601
                     /locus_tag="PK077_03730"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRN23438.1"
                     /translation="MKTPFLNRKSKGFTLIELLVVIAIILTLAGIAMTVVNGVMEKSK
                     VTTARSDCNGLQTAVENYMLDNGRVPVAWSGREMSKQARKDGQYYVTTAVDDDSRIMS
                     ILTNYMDRADEDRKLNPKKTAYFNTTTTDVEGAPGLFFESRGRVGLMDPWESPYYIVL
                     NANPDDRTPRVQVGSRMVTKNVAVYSTGKDKQGSTADGHINNPELTEDNVTSW"
     gene            8760..9827
                     /locus_tag="PK077_03735"
     CDS             8760..9827
                     /locus_tag="PK077_03735"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HRN23439.1"
                     /translation="MNWFKTDDIRIQDIEPLISPAILIKDYPATTEIAKMVATTRKNA
                     ENIISGHDDRLLVVVGPCSIHDPQAAVDYASRLKEQMARFEKDLVIIMRVYFEKPRTT
                     VGWKGLINDPFMNHTFDINRGLHMARGLLLRLGDMGVPAATEFLDTITPQYIADLITW
                     GAIGARTTESQVHRELASGLSMPVGFKNGTSGSLQIAVDAIVSSSCPHCFLSVTKQGV
                     SAIVSTTGNKSCHLILRGSSLGPNFDEDHVKEAEEALQKAGINNRIMIDCSHGNSCKD
                     YRKQPAVAANIAEQISSGSEQVVAVMIESNIVEGAQPLSSDLVYGKSITDQCIGWETT
                     VEVLETLAAAVRKRRAKRQEA"
     gene            complement(9970..10449)
                     /locus_tag="PK077_03740"
     CDS             complement(9970..10449)
                     /locus_tag="PK077_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23440.1"
                     /translation="MKFPAPLCAALLPVLFSCAEQVSRIEHVREVPLKTTVVPAEDVG
                     MHRTQATYLLHGAVSQQQRLQRLGQYYFVTWNDANPEQAATLVMMYRQGKTGSTVLTK
                     SISYPAGRKGGTQNSVFEFIGDEAREKGEILAWKLVLKDNRGKTISERHSYLWGDDH"
BASE COUNT         2258 a         2776 c         3251 g         2366 t
ORIGIN      
        1 ccgttccata gtgaagggga attttgatgt ggtgtacgat cttcaggaat cttcccgcac
       61 caggaaacgc tattatcccg ccgtgcgctt cctgctgaac catgacatgg actgggtggc
      121 ctgcaattcc ggagaacggc gcaaattgct gaagaagaaa tccttccggt gggggaaggt
      181 ggtacgggag ccgttccgtt tggaacgcgt tctgacggat ctttctttca tgcacgggga
      241 ggggctgcat tttgacgagc ttccggaacg ctatgtgatg atgattccgg gctgttcccc
      301 caaccatccc tacaagcgct ggcccgtgga aaatttctgt gtcctggcga aacgcctggc
      361 ggcgcggggc gtgtcctccg tcgtgatcgg cacccgggcg gaggctgtgg aagtggaggc
      421 cattgccgcc agctctcccc tggctgtcag ttttctgggc aagtcttccc tgatggatat
      481 tccccagatg gctttgcgtt ccctggcttg tgtaggcaat gatacggggc ctacacatat
      541 gtgtgcctat gccggggtgc ccgtgaccgc cattttctgc caccggacgc gcaactccgc
      601 cattactgcg cggtgcattt ccaacctggt ttcccccggc gccattgagg aaattacagt
      661 ggaccaggta tggtccgcgc tggagccttt tctgccgccg caggaaggat ttgaacaaat
      721 aagggcggca gattttccgc atggcttatg acggctcccg tgaacatttc cgtgctggtt
      781 ccggtttaca atgtggagcc gtacctggcc cagtgcctgg aaagcatttg ctcacagacg
      841 ctccgtgagc tggaaatagt ttgcgtggat gacgcttcca cggacggctc gctgtccatt
      901 ctgcgggaat tcgcggagcg ggacccgcgg gtgaaggtcg tgcaggctcc ggaaaacggc
      961 ggcctatccc gctcccggaa tctggcgatg agccatgctg tgggagagta tctgtttctg
     1021 gtggattccg acgactggct ggagacggat ttgctggagg agatgtaccg ccgtgcgaag
     1081 gcgctggatg ccgacaggct ggcatgcggg ttccggtatt attacgagtc cgcccccgac
     1141 cgggaggacc ggtttctgcc ggaggacatg gcccctccgg aaaaagggtg gcttccctgc
     1201 actccggaga ccattgggaa aatacatcat ggagcgggcg gcatgatgat caggcgttcc
     1261 attgtggaaa agcatggcat ccggttcccc gagggcgttg cctgtgaaga cttatatttc
     1321 cattacgccg tttttccgtg gtgcaggagg gtttgcgtcg tcagcagggc ggcttacgtt
     1381 taccgcaagc gggccggatc cattaccagc ggttttgcgt ccggcagttc cctccagtcg
     1441 ctggattacc tgacggtggc ggaactggtg ctgaaggaat ggaaagaagc cgggattctt
     1501 gaggaataca ggacggcatt tttgaaaatg ctggtaatgg gcgtgaggaa catccgcaaa
     1561 tacgcccctc atgccgtcca aaaggaggtt acccggaagg tggccgatat gctccgtcag
     1621 gaaaatctgt accgtcccgc agaggataat gcctgcctgt cccgccggga aggaaaattg
     1681 ctgaaagcct ggatgggcgg aaaatccggc ttggactttt cctattactg gaagaaaatg
     1741 cgcaaggcgg gagcccggtt gctgcgccgc tgacatcacc cgaatgaata atccgatgaa
     1801 aaagaatgaa tttgccgtcg tcctggccag tgacaacagg ggcatcctgc ctttgagcgt
     1861 tactgtcttt tctttgctga atactgccgg tccggagact ttttacaaaa tttacgttct
     1921 ttccgacggc attgacggag agaaccgggc aagcgtggag cggctggccg ctccgttcga
     1981 ttgccgtctg gagttcatag acgtttccgg cattttggaa gagcacgact tcccccatac
     2041 ggaacaatgg ccggtgccag cctggggacg cgtgttcatt ccggaattat tgaaggaaga
     2101 gcggggcaat attctgtatc tggacattga cgttctggtt tgccgggatt tgacggagct
     2161 gttccggacg aatatggacg gaaaagcagt aggggtggtg tttgagaatt tttccagacc
     2221 cggttcccat tttaacgaac gtcttgagat gccgctgacc tgcacgggat atttcaattc
     2281 cggcgtgctg ctgatgaatg tggatgtctt ccgggagagg aatctggtca gggctgtgct
     2341 ggattatgcc gtcactcacc gggacaggct gacgtgtccg gaccaggatg ccttgaacgg
     2401 agccctgtgc gagctgaccg tgccgctgca cccgcgctgg aactggcatg acggcttgac
     2461 gcgccgcatt ttgaaaaacg atccccggga acagttttgg cgcggcgtta cgccacgcca
     2521 ggcggtggaa gcggctttgg aaccgggcat tcttcattac cagggggtgc acaagccctg
     2581 gcggtataat tggcgttatg aaggggaacg ttacgaacgg gttatgcgtg aagccgggct
     2641 gctgcgcggt ccgctgcccg gaagaacgct cccggccgtc ttgaaaaagc acctttaccg
     2701 gcctgtttac cggatgacgg ccagaaagat tttaaggctg aaggaagggt ttgataaccg
     2761 gctcctttga aactatggaa cccggccgtt cttttccgtt ttctttatgg attgccgccg
     2821 gcgtggagcg gagggggagg gcaccgggaa ggaagggact gtatttttaa cgggaggaat
     2881 gggtcaatgc agttccagca gatagatttc cggcgggcag ttgaagcgga gggggagtgt
     2941 gcttgtcccc agccccttgg tgatcaggta ggtattgccg ccggagatat gccagggata
     3001 ggtgtagggt agggcatggt ccgggctggc ttcaaaccgc aggaggccga aaggaaggca
     3061 aacctggcct ccgtgggtgt gcccgctgat gacgatgtcc acgtctccat gggaaagtat
     3121 gcggtccgtt tccggacggt gggaaagcag gaaatggggg atgtccggcg actggcgttt
     3181 gggcagccgt tttctgacat tgcgcgtcgt atccaatatt ccggaaagct ggagctgcct
     3241 tccgtccttg aacgtgacaa gagagctctg gtcttccagc acggtgattc ccgcctgttt
     3301 cagttccctg ctccagttgt tccagccgaa ggtgaaatca tggtttccct gaatagcaaa
     3361 gaccccgcag ggagccgtca ggcctttcag cagccgggcg gccgtggcgg gatccatgct
     3421 ggcgttttcg ctgcttcccc gggcatagtc cccaaccagc agaatcaggt ccggccggag
     3481 ccgcatgatc ctctgaacat agcgcagggc cctcacttca tcaaacgcgg tgggcatcag
     3541 gtgcaaatcg ccggcaataa cgactttaac gggtgttccc ttccctttcc actgggggac
     3601 acggtagtcc actttcctga caaacagaag attgggttct accaggtacg cccaggtcat
     3661 gccggccacg ccgagcgcgc agaggctcag caggagaatg ccgaaaaacc ggagtatttt
     3721 gacggaaaag ggcatgaaaa ggatacagct tggcagagat tataattaca gataaccccg
     3781 ggcgagcgat ttatgcgtct tgccattttg ggaaaggcgt ctccttggga gattttcgca
     3841 aatttgattg acgggcgccc gcatggtatg cttttatgct ccaagatatt tccatatcct
     3901 ttatcaccca tcatttaacc acgcatatga agaagattac cgttatggct cttgccgctt
     3961 ttgcggcgag catcatggct tcctgctgct gccaggatca gggtgctccg cccctgaccc
     4021 ccctgcccaa gttcaatgac ctgaaccagc ccgccgtgga tgtggctccg gtggtcatga
     4081 agtccaagaa gtaatcctct tccgggatta agtacgactt ttcaagccgc acttggtgat
     4141 tgtccaggtg cggctttttc tataaaaaca cggcagtgca tatggaagat tggaaacatt
     4201 gtccccaatt cgcgcctttg gcagatctga tccgcagcca cggcagtttt gcggtaattg
     4261 cgcatgttca tccggacggc gacgctatcg gctccacgct ggctttgggg gaagccctga
     4321 agcaaatggg aaagaaggtg gtgatgatga acgaggacgg ggtgccttcc aatctggcct
     4381 ttatgccggg tgtggaaaat attattccca cgcctgatga gccggtggat gtggaagtag
     4441 ccatttccgt ggataacggc gcgctcaaga ggctgggaga acgcagcctg gaggctttgg
     4501 cgggagtgaa ggtgtgggcg aacatcgacc accatcggac gaacgaactg tttggagatg
     4561 tgcagtgcgt tttgccggac gaatgcgcca cgggggcggt tttatattac tttttcaagt
     4621 atctgggcgt tcccgtcact ccggtgatgc gagacgccct ttacgtggcc gtcagcacgg
     4681 atacggggtc tttccagtac cagatgacga cggcggccgt catggaactg gctgcggatt
     4741 tgatccggat gggcgttgac gtccaggaca tcaaccgcca gctttaccag gaaaagccgt
     4801 gggtgaaaat gcagttgatg cgtgaggcgc tgaacggaat gaagctgacg ccggacggca
     4861 ggatttgcat gttttgcctg acgaatgagg caaaagcccg gattggaagc cgtccggaag
     4921 atacggaagg cctgattgac ctcctgcgtt ccgtgcaggg agtctggctg gccgcttatc
     4981 tggaagaaac ggaggacgat ccccgcatcc gtatttccct gagatccaag acgcctgaaa
     5041 tatccgtcgc ggagctggcc tcccgtttcg gcggaggcgg ccattccatg gccgccggag
     5101 tgcgtatccg ggggcctatt gaagaagtgc gccttactat cctgaaggct atgcgggaag
     5161 aagtggaacg agtactccga cagaagattt catgaatgtt tcagattcca tccaggttcc
     5221 ttcgggcgtc ctgttgattg acaaggcgcg ggacatgact tcccacgatg tggtagcgat
     5281 tgcgcggcgt tccctgggaa ttaagaaaat aggccactgc ggcacgctgg atcccatggc
     5341 cacggggctg ctgatgctgg tggtgggaaa ggcgaccaag cttcaggaca agttgatgtg
     5401 tgaacacaag gaatacgccg gaactctcat gctgggcgtg gagacttcct cccaggatgc
     5461 catgggagaa atagtggcgg agtattccgt ggacggagtg acggaacagg ccgtgcgcga
     5521 ggcgtttgac cggtttgacg gcgcttttga acaaatccct ccgatggttt ccgctattaa
     5581 aaagggcggg gttccgctgt acaagctggc ccggaaaggt caggaagttg tgcgggaacc
     5641 gcggcctgtg gaagtgtttg gccacggaat ttcacgcgtg gccattccgg aagtggattt
     5701 taccgttcag tgttccaaag gtttttatgt gcggtcctat gcttatgata ttgggaaggc
     5761 attgggctgc ggagcgcatt tgagcgccct ccggcgcacc aggtccggtt cttttacgct
     5821 ggaccgcgcc attacggtgg acaccctgaa aaccgccccc agggaagagc tgttccgggc
     5881 catgctttcc ctgaaggagc tggcggatat tctgattgcc gggtagcata tggtcattta
     5941 ccgggaattc aagggattga aaagccttgc cgcccccgta cactgggcca tggggatgtt
     6001 tgacggggtg catctgggac acgccggagt gattgccgcg gcggtgaaag gggctgctct
     6061 tgacggagga attcccgccg tgctgacatt ccgcactcat ccgctggccc gggtgaggcc
     6121 ggaaagcgtg ccgcccgcca tcatggggga agatgctgaa aaatttgcct tgatggaaga
     6181 gctgggggtg aaggcggtgc tgtcgctgga attttcttcc aggctggcgt ccatgagccc
     6241 ggaagagttt atcggggaac tttgttcctc ctgcctggtg gctcagattg ccgtgggaga
     6301 ggactggcat tttggccgcg gcagggcagg gaatgtagaa accctgcgcc tgctgtcctg
     6361 ccgctacggg ttccgggtga cggccgttcc ttctattctg caggacgggg agcgcataag
     6421 cagcacccgg atccgcgaag ccgtgcgcgc ggcggatttt ttacaggctg ccggcatgct
     6481 tggccgtccc taccgctgga aggggccggt gattcacggg cgccaactgg ggcgcctgct
     6541 ggactacccc acggccaata tgagacccgg ttgcgggttg cagcctcccc atggcgttta
     6601 cgccgtgcgc gcccgggtgg ccggcaaaaa atacggaggc gtagccaatc tgggggtgcg
     6661 ccccaccgtg gaaggtgaga cgggggaact tttgctggaa acgcatttat tcggccaccc
     6721 gggggatatt tacgggcggg agatggaagt ggaaccggtc cgtttcctaa ggccggaaat
     6781 caaattccct tctttggaag aattgaaaag ccagcttgcc cgggatgctg cccatgccgc
     6841 ggaagcgctg gaggaggggg agtaagacac agaaaaccgt aaaatggagg atgacggctt
     6901 gaccggaggg gcgatcgggg gtttgttgtt cgaacgtcat gttggttgag cagttaaaat
     6961 caaaaattca tcgcgccatg attacccgtg gcgatgttca atatgaaggc agtattgaaa
     7021 ttcctgccga tttgatggcc gccgtggatt tgtggcccgg ggaaaaagtt ctcgtggcgt
     7081 ccgtcacgtc cgggaaccgt ctggaaacat atgctctcca ggggcctgcc ggcacgggga
     7141 acatcatcat gaacggcggt gcggcccatt taatcaagac gggcgaacgg gtggtcatta
     7201 tgagttttgc cttgtcggac aagcccatca tccccaagaa aatcgtctgc aacgagcata
     7261 acgaaatcat ttcctagact ttacaaaccg ttcgaattca cacatactcc ccgcgctcat
     7321 gaaccttttt ttagcagatt cactcaatat cagcattcag ttgctttttg ccgttttggt
     7381 gattgtatca cttctccttc tgggcgtagt gcttatgcag aggcccaagc aggaagggct
     7441 gggagccgcc tttggcgcag ccattacgga ccaggccttt ggcgcccgga cgacggatgt
     7501 gctgaaaaaa gccacggtgt acttcggaac tgcgttcatg gtcctttgcc tggggctggg
     7561 catgctgatc aaccggcagc acgttaagag ttcggaaagc ctgttgagcc cggaaatgat
     7621 gaaggctgcc gccaggcagg aagcctccgt tcccgccaag acgccggaag aactccagca
     7681 ggaggagttg cgcaggcgcg ccgctgaggc ggaagccgct tcttctcagg ctccggctcc
     7741 ctccgtgacc gaagccccgg cccctgcttc cacagaaact cctgtcccct ccaactaaag
     7801 gagcattctt tttaaacacc ccggctgccg gaaggcgccg gggtgtttgt gcaccggggt
     7861 tattccgtct gctgccgttc cgggaacagg ctttgaaatt ggcggcaccg gttgttttcc
     7921 gcttgccctg aaccgtaatt gcgctactct tggcaaagta tgaagacacc ttttttgaac
     7981 cgtaaatcca agggattcac gttaattgaa ttgcttgtgg tgatcgccat tatcctgacg
     8041 ctggccggca tcgccatgac ggtggtgaac ggcgtgatgg aaaaatccaa ggttaccacg
     8101 gcgcggagcg actgcaacgg cctgcagacg gctgtggaaa attacatgct ggacaacggc
     8161 cgcgttcccg tggcctggag cggccgggaa atgtccaagc aggcccgcaa ggacggacag
     8221 tattacgtga caaccgccgt ggacgatgat agccgcatca tgagcatcct gaccaattat
     8281 atggaccggg cggatgagga cagaaagctg aaccccaaga aaacggctta tttcaatacg
     8341 acgactacgg atgtggaagg cgctcccggc ctgttttttg aaagccgcgg ccgtgtgggc
     8401 ctgatggatc cctgggaatc tccctattac attgttttga atgccaaccc tgacgaccgt
     8461 actccccgtg tccaggtagg ttcccggatg gtgaccaaga acgtggccgt ttacagcacc
     8521 ggcaaggaca agcagggcag cacggcggac ggccatatca acaatccgga attgacggaa
     8581 gacaatgtga cttcctggta atgcatgacg gcgcctcttg tcgggaggcg tggccgcttc
     8641 attttgcggg gcttccggct tatggccgtg aggccccgcg gtatattcag gcataaagcc
     8701 cgcaaaccgt atttttttct tttcaacttc tctcaaaaac ctctcaataa gccccagaca
     8761 tgaactggtt caaaacagac gacatacgca ttcaagacat tgagcccctg atttcccccg
     8821 ccattttaat caaggattat ccggcaacca cggagattgc caaaatggtg gctacgaccc
     8881 gcaagaatgc ggagaacatc atttccggcc acgacgaccg cctgctggtg gtggtggggc
     8941 catgctccat ccatgatccc caggctgccg tggattatgc ctcccgcctg aaggaacaaa
     9001 tggcgcgctt tgaaaaggac ctggtgatca tcatgcgcgt gtattttgaa aagccccgca
     9061 ccaccgtggg ctggaagggc ctcatcaacg acccgttcat gaaccatacc tttgacatca
     9121 accgcggcct ccacatggcc cgcgggctgc tgctgcgcct gggggatatg ggagtgcccg
     9181 cagctaccga gttcctggac accatcacgc cgcagtacat tgcggacctg atcacgtggg
     9241 gcgccatcgg cgcgcgcacc acggaaagcc aggtacaccg tgaactggct tccgggcttt
     9301 ccatgcctgt gggattcaag aatggcacca gcggcagcct gcaaatcgct gtggacgcca
     9361 ttgtttcctc ctcctgtccg cactgcttcc tttccgtgac caagcaggga gtttccgcca
     9421 ttgtttccac tacgggcaat aaatcctgcc acctgatcct gcgcggttcc tccctggggc
     9481 cgaactttga tgaagatcat gtaaaggaag cggaagaagc cttgcagaag gccggcatca
     9541 ataaccgcat catgatagac tgctcccacg gaaacagttg caaggattat cgcaaacagc
     9601 cggctgtggc cgccaatatc gcggaacaga tatccagcgg gtccgaacag gttgttgccg
     9661 tgatgattga aagcaacatt gtggaaggag cccagccgtt gagttccgac ctggtgtacg
     9721 gcaagagcat tacggaccaa tgcattgggt gggagacgac agtggaagtg ttggaaaccc
     9781 ttgccgccgc tgtccgcaaa cgccgtgcca aacggcagga agcgtaagcg tgcccatatt
     9841 ccggaggatt attaacgaag ttggaggctg gcgcggccat gccttggctg gcgagactgc
     9901 cggaaactac aggtctcatc cttcatatcc ggctgatttt actggctctt cggtgttttt
     9961 gccgaagagt caatgatcgt ccccccacag ataagaatgg cgttcggaaa tcgttttgcc
    10021 gcggttatcc ttcaggacga gcttccatgc gaggatttcc cctttttctc tggcctcatc
    10081 cccgatgaat tcgaaaacgg agttttgcgt gccgcctttt ctcccggccg gataggaaat
    10141 ggattttgtc aggacggttg aacctgtctt tccctggcgg tacatcatga ccagggttgc
    10201 cgcctgttcc ggattggcat cgttccatgt gacgaagtag tattgaccca gacgctgaag
    10261 gcgctgttgc tgggagacgg cgccatgcag cagataggtg gcctgagtgc ggtgcatgcc
    10321 tacgtcttcc gcaggaacca ccgtggtttt taagggcacc tcccgaacgt gttcaatccg
    10381 ggaaacctgc tccgcgcagg aaaacaggac cggcagcagg gcggcgcaga gaggagcagg
    10441 aaatttcatg aatccattca atacatatcc gactggggag tcaacccaaa ggtagggaag
    10501 cattcagcat ggaatgcagt atggatgcgg aaaggatgcg gatatttcgg cagggaaatt
    10561 gttccgccgc cgaatcctgc ggactggttt tatttccatc aaggggcggg acaccgggaa
    10621 tagaatctcc tctggaaaag cggaagatgt t
//
LOCUS       DAOMSU010000053        10602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_375_length_10602_cov_3.504124, whole genome shotgun sequence.
ACCESSION   DAOMSU010000053 DAOMSU010000000
VERSION     DAOMSU010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10602
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_375_length_10602_cov_3.504124"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /gene="eno"
                     /locus_tag="PK077_03745"
     CDS             <1..1124
                     /gene="eno"
                     /locus_tag="PK077_03745"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRN23441.1"
                     /translation="DATRYQGKGVTKAVDNIRNIIAPALIGLEATEQTTADALMNSLD
                     GTFNKAKLGANAILGVSLALAKAAAAQLHQPLYRYLGGPNAKVLPVPMMNIINGGAHS
                     DAPIDFQEFMIVPSGAPTFREALRYGAEVFHALKSVLHDRGLSTAVGDEGGFAPRLNS
                     AEDALDCIAQAVDKAGYRFGEDISIALDVASSEFYEPESGLYVFKKSDGSRRTAAELT
                     DYYVELKKKYPIVSIEDGCAENDWDGWKTLTEKLGKSTQLVGDDLFVTNVEFLRKGIH
                     LGVGNSILVKVNQIGSLTETLDAVELAMGNRYTAVISHRSGETEDATIADIAVGTNAG
                     QIKTGSLSRSDRMAKYNRLLRIEEELGESARYGNGFGLF"
     assembly_gap    1421..1434
                     /estimated_length=14
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1435..2802
                     /locus_tag="PK077_03750"
     CDS             <1435..2802
                     /locus_tag="PK077_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23442.1"
                     /translation="ACGLYVCARHLPAQRLPLSGRRLAALGLLSFFCLMLVLLCGFTG
                     HCQQHADFIIRNAVYERLAANSWPLVTEDGHHFIYYLGHWLPPALAASFCPASWAPWL
                     LALWTFLGLELALLAATVRWGIRKTARWALILLCLGSPAAVPDCLGIPLSSLFAEYHA
                     QMVLFIGMPVQLFNTFNHAVPALLCAVFVLTRSLPPSGYYLAGTLLLPSSPLGALLLL
                     PYMAYETLFRLPAARKPLSRLRSLLGQPVFWLAALCTAVMAVFYSQLGGGGQFSCLFD
                     AKYAEIYHYGQQRLLLHPDSVKYASFLLALALGILLPGALLFPKCRKNPLYYITLGMM
                     AGSLFFRTGIMNNELLFKAPAVLYPFLSLLFLRALRRGDVKYRALLILYLVFSALPNL
                     QCIMEKAETFSTRASIMRKHWQTAGGTPDCPGTLIRRQFMKKDGHPLPSWLFKTGIKP
                     QSRQQ"
     gene            complement(2803..3492)
                     /locus_tag="PK077_03755"
     CDS             complement(2803..3492)
                     /locus_tag="PK077_03755"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HRN23443.1"
                     /translation="MDDEMPLKREDLRKRAEEKAAKLAAEGMELRPVTVSGRTLAKRF
                     WGKEWMKSLSACEVYGMRLAPGRTYLRYGCVLDLKTVPGRIDALVMGEHLYEVCIQAS
                     PPDEEALARLRARCAGQIGSWIDLLKGNLSPELLEILCAPEGGLFPAPEEWRFSCSCP
                     DWADLCKHAAAVLYAFGVMLDKQPELLFTLRGMDSSVLIPKAPEPVPGGEDALDVDAG
                     SLSDIFGISLD"
     gene            3684..5051
                     /gene="argH"
                     /locus_tag="PK077_03760"
     CDS             3684..5051
                     /gene="argH"
                     /locus_tag="PK077_03760"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HRN23444.1"
                     /translation="MWKGRFSKPTADLVQRYGESVSYDWRLFRQDIAGSIAHARAQLK
                     AGLLSQEEFNAIESGLKDILKDIEAGNFSWSRELEDVHMNIESELTRRIGAPGAKLHT
                     ARSRNDQVATDTRLYCRAEIDEILGKVRHLQRALVMKAKEYADAMMPGYTHLQRAQPV
                     TMGHHLLAYVEMLDRDADRLKDCRRRLNVSPLGSGAIAGSTICLDRHGIAVELGFDAV
                     TENSMDAIADRDYIMEILFDLAVVGAHLSRMSEDVILWCTAEFGFASLSDAYTTGSSL
                     MPQKKNPDVAELTRGKTGRLYGNLTAVMVAVKGLPLTYNRDLQEDKEPLFDSIDTVKL
                     ALDVNAEMIAAMTVNTARAREAASDPMLLATDLADYLVRRGVPFRKAHELVGKAVAMS
                     ISTGTPLNKLSDEQFASISNAYGTDARDVFELTKAFAQRTNPGAPNSDNTRRRIAYWE
                     GILSK"
     gene            5188..6867
                     /gene="sulP"
                     /locus_tag="PK077_03765"
     CDS             5188..6867
                     /gene="sulP"
                     /locus_tag="PK077_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HRN23445.1"
                     /translation="MFKPALLSSLKTYTKQTFLADLFAGLTVGVVAIPLAMAFAIACG
                     LSPTQGLITAIVAGFLISLFSGSKYQIGGPTGAFVIIIMGVLEQYHASGLLVCTLMAG
                     LFLIIFGFCRMGALIRFIPFPVTTGFTSGIAVVIFSTQIKDIFGLTITEKIPGEFIEK
                     WACYFHYFHTINWAALGLAAGTVIITLLSRRFWPRIPAMLVGMLGMTAVSVAFSLPVT
                     TIGQAFGSLPNTLPLPSLPSIDWSTLGALTAPAFTIALLAAIESLLSASVADGMTGGR
                     HKPNMELIAQGIGNIGSALFGGIPATGAIARTATNIKAGAKSPVSGMIHALTLLAILM
                     AFAHYAQQIPLAVLAGILTVVCYNMSEIHTFSRLLKGPRQDAAVLVITFLLTVFVDLV
                     VAVEVGVVLAALLFMGRMAQISDVSAIKNELLENDEEDDGNRSAAKLDIPEGVEVFDV
                     KGPFFFGAVEQFKDQVLETLEHDTKVVILRMRLVPALDATGLNVLSDFCHQCREHGST
                     LLVCGVQPQPLDVIRHAPFYRELKRYNICENIDAALNRARKIINGPAPKHL"
     gene            complement(7263..7790)
                     /locus_tag="PK077_03770"
     CDS             complement(7263..7790)
                     /locus_tag="PK077_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23446.1"
                     /translation="MIGFRNNRPLLKLGDCLITEYGEQWLADALEHAARRAGTTLPFK
                     EDLLASVRYYLEHECDLSVMKVSELYARLRRMLTEVGLEHLARELREEMPPMTVCVAE
                     IARSVPFWLFFACELKKQVEELRGHGITRYCFTGRKECVMALRGRKRWDRSCQSLLED
                     LDFLLSRYEEQAADC"
     gene            complement(7803..8294)
                     /gene="nrdR"
                     /locus_tag="PK077_03775"
     CDS             complement(7803..8294)
                     /gene="nrdR"
                     /locus_tag="PK077_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRN23447.1"
                     /translation="MRCVQCGHLEDKVIDSRMSKDGTTIRRRRVCLRCDYRYTTYEQI
                     ERTELRVVKRDNLREALNREKILRGLVKACEKRPVSMDRLDRAVEEIISELHRDHLRE
                     VPSSEIGKKVIEKLYAIDPVAYIRYVSVYRQFSNVEEFIQEITQMRHQTLIDPLQRKL
                     PIL"
     gene            complement(8574..10067)
                     /locus_tag="PK077_03780"
     CDS             complement(8574..10067)
                     /locus_tag="PK077_03780"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HRN23448.1"
                     /translation="MKIFLLTGQSNSLGAVKGSPASPELLKKYEPKETLYWHENFGQR
                     EGVFPGASTSWEQVRPAMPRYNGNLCMGPEYGFAFTLEKNGWFKDADVAVVKASRDGG
                     DNSHWRKNGQAYRTLVQAVKNACAGVDRSKYSKVEFAGLLYLQGESNAGTSVPESASR
                     FLELLGNLAADLKPYGDTSALAAQKAVLGENANWAGKNESDPETGNLTGGLEGRDTEV
                     QGKTTRQVMKDLAESRPSLGYAPTRDLPKLTAGDQMGVHYSGQSQISIGARFAYEAAR
                     LAGKDTGSVRSGRYDLPLGSPDAWMNRKMPGKNVCVWNVASSVKPSLVSGGVKLFGIR
                     VEDPAVKTVIVRSKGSSGDRLVIGPGGIRLAEGKNLQLRTNVQLAGRQSWNIPGGSAV
                     EIKPSPVQEKAMPVRLSGQAEVHVTQAEGGGETAEAARVVLEQVLPSALKCSWTLSGK
                     VEMTLKGMEGKAVNLGKVFVKQGAVLNLNGSRPVAGSVVNQGGMVNP"
     gene            10357..10503
                     /locus_tag="PK077_03785"
     CDS             10357..10503
                     /locus_tag="PK077_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23449.1"
                     /translation="MGSFFASLSRLYQASCLLRLRMTSPAKPSRLREAVAGSGTKYAD
                     MAAS"
BASE COUNT         2292 a         3371 c         2686 g         2239 t
ORIGIN      
        1 gcgacgcaac ccgctaccag ggcaagggag tcaccaaggc cgtagacaac atccgcaaca
       61 tcattgctcc ggccctgatc ggtctggaag ccacggagca aaccacggcg gatgccctca
      121 tgaactccct ggacggcacg ttcaacaaag ccaaactggg ggccaacgcc attctgggcg
      181 tttccctggc gcttgccaag gctgcggcgg cccagctgca ccagcccctg taccgctacc
      241 tgggcggccc gaacgccaag gttctgcccg tccccatgat gaacatcatc aacggaggcg
      301 cccactccga cgcgcctatt gatttccagg aattcatgat cgtcccgtcc ggcgctccca
      361 ccttccggga agccctgcgt tatggcgcgg aagttttcca tgcgctgaaa tccgttcttc
      421 atgaccgcgg cctttccacc gccgtgggcg acgaaggcgg cttcgccccc aggctgaact
      481 ctgcggaaga cgccctggat tgcatcgccc aggcggtaga caaggccggt taccggttcg
      541 gggaggacat ttccattgcc ctggacgtgg cttcctccga attttatgaa ccggaatccg
      601 ggctgtatgt tttcaagaaa tccgacggca gccgcagaac cgccgcagag ctgacggact
      661 actatgtgga actgaagaaa aaatatccca tcgtctccat tgaagacggc tgtgcggaaa
      721 acgactggga cggatggaaa accctgacgg aaaaacttgg caaaagcact cagcttgtcg
      781 gagacgatct ctttgtcacc aacgtggaat tcctcagaaa aggcattcat ctgggcgtgg
      841 gcaactccat cctggtgaag gtcaatcaaa tcggctcgct gacggaaacg ctggatgccg
      901 tggagctggc tatgggcaac cgctataccg ccgttatttc ccaccgttcc ggagaaacgg
      961 aggacgccac catcgcggac attgccgtag gaaccaacgc cggacaaatc aaaaccggct
     1021 ctttgagccg ttccgaccgt atggccaaat acaaccgcct tctccggatt gaagaagaac
     1081 tgggagaatc cgcccgctac ggcaacggat ttggcctgtt ctgacctttt tgctggaact
     1141 ggtatcaagc aacgccaacc cccggccgcc gcgaggcggt gcggggcttt ttcattctcc
     1201 ggaggaaact gcatcctttt ctttggaaca aaatatgcca tgctggcctt ttggattcct
     1261 ccctgccgac cagtttcccg ttccgcgcca tgtctgcccg cttctttctg cccgcacgcc
     1321 ttccgctatg cgcgggactg ttgtatctgg cgttgcccct cctccttttt ctgcagggat
     1381 gggtgcatcc ggccttttcc gcgccgcttt gcgccgcact nnnnnnnnnn nnnngcatgc
     1441 gggctgtacg tctgcgcccg gcatcttccg gcacaaaggc ttccgttaag cgggcgcaga
     1501 ttggccgcgc tgggtctcct ctccttcttc tgcctgatgc tggtgctgtt gtgcggattc
     1561 acgggccact gccagcaaca cgcggatttt atcatccgca atgccgttta tgaacgcctt
     1621 gcagcaaatt cctggcccct cgtcacggag gacggacatc atttcatcta ctacctgggc
     1681 cattggctgc ctccggctct ggcggcttct ttctgcccgg catcatgggc tccctggcta
     1741 ctggccctgt ggacctttct tgggctggaa ctggcgctgc tggcggcaac cgtccgctgg
     1801 ggaatccgga agacggcccg ctgggcactg atactcctct gcctcggctc tccggcagcg
     1861 gtgccggact gcctgggaat tcccctctcc tccctgtttg cggaatacca tgcccagatg
     1921 gtcctgttca tcggcatgcc cgtccaactc ttcaacacat tcaatcacgc cgttccggcg
     1981 ctattgtgcg ccgtattcgt cctgacgcgc tccctgcctc cctccgggta ttatctcgcg
     2041 ggaactcttc tgctgccgtc ctccccgctg ggcgcccttc tgctccttcc gtatatggcg
     2101 tatgaaaccc tgttccgcct cccggcggca cggaaaccgc tttcccgtct ccgttccctt
     2161 ctgggccagc cggttttctg gctcgcggcc ctgtgcacgg ccgtcatggc cgtcttttac
     2221 tcccaattgg gcggaggagg gcaattctcc tgcctgtttg atgcaaaata tgcggaaatt
     2281 taccattacg ggcaacagcg gcttctgctc catcccgatt ccgtcaaata cgccagcttc
     2341 ctgctggccc tggccctggg gatactgctg ccgggggcgc tcctcttccc aaaatgccgg
     2401 aaaaacccgt tgtactacat cacgctgggc atgatggcag gcagcctctt tttccgcacg
     2461 ggcatcatga acaatgaact tctctttaaa gctccggccg tcctctatcc cttcctttcc
     2521 ctgctctttc tccgggccct ccgccgggga gacgtgaaat accgggccct cctgattctt
     2581 tatctcgttt tttccgccct cccgaacctg caatgcatca tggaaaaagc ggaaacattc
     2641 tccacccggg cgagcatcat gcggaaacat tggcaaacgg ccggcggcac gcctgactgc
     2701 ccgggcaccc tcatccgccg ccagttcatg aaaaaggacg gccatcccct tccctcctgg
     2761 ctattcaaaa ccggaataaa accacaatca cggcaacaat aatcaatcca ggctgatgcc
     2821 gaatatatcc gaaagggaac cggcatccac atccagtgcg tcttctcctc cgggaacggg
     2881 ttccggagct ttcggaatca aaacggagga atccattccg cgcagggtga acaacaactc
     2941 cggctgctta tccagcatca cgccgaaagc gtacaggact gcggccgcat gcttgcacaa
     3001 atccgcccaa tccggacagg aacaggaaaa acgccactct tctggcgcgg gaaacaggcc
     3061 gccttccgga gcgcacagga tttccagcag ctcgggagac aaattccctt tcagcagatc
     3121 aatccaggaa ccaatctgcc cggcgcatcg tgcgcgcagc cgggcaagcg cctcttcatc
     3181 cgggggggag gcctggatgc aaacctcata caaatgttca cccatcacca gagcatcgat
     3241 gcggccggga accgttttaa ggtccagaac acagccatag cgcaggtagg tccggccggg
     3301 ggccagccgc atcccgtaca cctcgcacgc ggacagactc ttcatccatt ccttccccca
     3361 gaaccttttc gccagggtac gcccggaaac cgttacgggg cgcagttcca tcccctccgc
     3421 agccagtttc gccgcctttt cctccgcgcg tttccgcaaa tcttcccgtt tcagaggcat
     3481 ttcgtcatcc atacgcacgg catcgtaata acatatccgc ccccatccgc cacagggaaa
     3541 agacagccat gctccgtttc tctcttttcc ggctccactc cggttttcca ggcatgcgga
     3601 tttttcccag ttccgcggaa tctgcctcca ttacccattt caggctttca aaacggaagc
     3661 tcctggggca caacatgtgg gtcatgtgga aaggcagatt ttctaaaccc accgctgatc
     3721 tggttcaacg ctacggcgaa tccgtctcct acgactggag gcttttccgc caggacattg
     3781 ccggatccat cgcccatgcg cgggcccagc tcaaggctgg cctgctttcg caggaagaat
     3841 tcaacgccat tgaatccggc ctgaaagaca tcctgaaaga cattgaagca ggcaacttct
     3901 cgtggagccg ggaactggag gacgtgcaca tgaacattga aagcgaactc acccggcgca
     3961 tcggcgctcc gggcgccaag ctgcataccg cacgctcccg caatgaccag gtagctacgg
     4021 acacgcggct ttactgccgg gcggaaatcg atgaaatttt gggaaaagtg cgccatctgc
     4081 agcgcgcgct ggtcatgaag gcgaaggaat acgcagatgc catgatgccg ggctatactc
     4141 acctgcagcg cgcccagccc gtcaccatgg gccatcacct gctggcttac gtggaaatgc
     4201 tggaccggga tgcggaccgc ctcaaggact gccgcaggcg cctgaacgtc tccccgctcg
     4261 gttccggagc catcgccggt tccaccatct gcctggaccg ccacggaatt gccgtggaac
     4321 tgggttttga cgcagttaca gaaaactcca tggatgcaat tgccgaccgg gattacatca
     4381 tggaaatact ttttgacctg gccgtagtcg gagcccatct ctcccgcatg agcgaggacg
     4441 tcatcctgtg gtgcacggcg gaatttggct tcgcttcctt gtccgacgcc tatacgacag
     4501 gttcatccct gatgccccag aaaaaaaatc cggatgtcgc ggaactcacc cgcggaaaaa
     4561 cggggcggct gtacggtaac ctcaccgccg tcatggtggc cgtcaaaggg cttcccctga
     4621 cttacaaccg cgacctccag gaagacaagg aacccctgtt cgattccatt gatacggtta
     4681 aactggcctt ggacgtcaat gcggaaatga tagccgccat gacggtgaac acggccaggg
     4741 ccagggaagc ggcgtccgat cccatgctgc tggccacgga cttggcggat tatctggtgc
     4801 gccgcggggt ccccttccgg aaggcccatg agctggtcgg gaaggcagtc gccatgagca
     4861 tctccacggg tacgccgctc aacaagctga gcgatgaaca attcgcctcc atttcaaatg
     4921 catatggaac agacgcgcgc gacgtatttg aattgacaaa agccttcgcc cagcgtacca
     4981 acccgggggc tcccaattcc gacaacaccc gccgccgcat cgcctactgg gaaggcatcc
     5041 tttccaagta acgccgcctg cggaggcgga cgggaacgaa gagagcctgc cgtccgtctc
     5101 cgcggggcgc ctccgggtgc aggaagggga cgcttcaacc ccgccggcgg cggccacaac
     5161 cgcagccggc caaccatccc ggcagccatg ttcaaacctg ctcttctctc ctctctcaaa
     5221 acttatacca aacaaacctt tctggcggac ctttttgcgg gactgaccgt cggtgtagta
     5281 gccattccgc tggccatggc ctttgccatc gcatgcggac tctccccaac ccagggcctc
     5341 atcaccgcca ttgtggccgg gttcctcatc tccctgttca gcggaagcaa atatcaaata
     5401 ggcggcccca ccggagcctt cgtgatcatc atcatgggcg tcctggagca ataccacgca
     5461 tccggcctgc tggtctgcac attgatggcg ggcctcttcc tcatcatctt tgggttctgc
     5521 cgcatggggg cgctcatccg ctttattcca ttccctgtca ccacagggtt cacctccggc
     5581 atcgccgtgg taatcttttc cacgcaaatt aaagacatct tcggcctcac catcacggaa
     5641 aaaattcccg gagagttcat tgaaaaatgg gcgtgttact tccattactt ccacaccatc
     5701 aactgggcgg cgctggggct ggccgccggc accgtaatca ttaccctgct gagccgccgc
     5761 ttctggccca gaataccggc catgctggtg ggcatgctgg gcatgacggc cgtttccgtg
     5821 gcgttttcgt tgcctgtgac aaccatcggg caagccttcg gcagcctccc gaatacgctc
     5881 cccctgccct ccctgcccag cattgactgg agcaccctgg gggcgctgac ggcccctgct
     5941 ttcaccatcg cgctgctggc ggcgatcgaa tccctgttaa gcgcctccgt agcggacggc
     6001 atgaccggag ggcgccacaa gcccaacatg gagctgattg cacaaggtat cggcaacatc
     6061 ggatccgccc tgtttggcgg cattccggcc accggagcca ttgcccgcac cgccactaac
     6121 atcaaggctg gagctaaaag cccggtttcc ggtatgattc acgccctgac cctgctggcc
     6181 attctgatgg cctttgccca ctatgcccag cagattcccc tggctgtcct ggcgggcatt
     6241 ctgacggtag tgtgctacaa catgagtgaa atacacacgt tcagccgtct actgaaaggg
     6301 cccaggcagg atgcggcggt gctggtaatc accttcctgc tgaccgtgtt tgtggacctc
     6361 gttgtagccg tggaagtagg cgtggtgctg gccgccctgc tcttcatggg ccgcatggcc
     6421 caaatcagcg atgtttccgc catcaaaaac gaactgctgg aaaatgatga ggaagatgat
     6481 ggaaaccgct ctgccgccaa gctggacatc ccggaaggcg tggaagtttt cgacgtgaaa
     6541 ggtcccttct tcttcggtgc cgtggagcaa ttcaaggacc aggttctgga aacgctggaa
     6601 catgatacca aggtggttat cctgcgcatg cgcctggttc ccgcgctgga cgccaccggc
     6661 ctgaacgtcc tttccgactt ctgccaccag tgccgggaac acggttccac tctgctggtt
     6721 tgcggcgtgc agccccagcc tctggacgtc atccgccacg cgccctttta ccgggagctg
     6781 aaacgctaca atatctgcga gaatattgac gccgccctga accgggcccg caaaatcatc
     6841 aacggccctg cgcccaaaca cctgtaacag cggccgttcc cccgtgcggt cagggagcaa
     6901 gacctcactc tgccttattc acggccttcc ggtacgggtg aggctgctcc tgctgcaaac
     6961 agccagaaat aacaacctcg gggaaaatga gggaaggccc cttgcattcc ctcatccggc
     7021 tcatctccgg gaatcaggcg gcacaatacg gcctgttaac tcttattggg ccttccggca
     7081 aaacaggatg cccgagcatg gatcaacggg ccccgcaagc ccgttcccgg taaagtgaaa
     7141 cagcctcgca tgctcacgaa aagcagaaac gcgctgcact ccgaaccggc tccgtcagcc
     7201 gggaaagcgg aacctgttga attgcgcagc taaatactcc aatacggcac ggataagttc
     7261 cgttaacaat ccgccgcctg ctcctcatag cgggaaagaa ggaagtccag gtcttccagc
     7321 agagactggc aggaacggtc ccagcgcttc cttccccgga gagccatcac gcattccttc
     7381 ctgcctgtga agcaatacct ggtaatgcca tgcccccgca attcctccac ttgctttttc
     7441 agctcgcagg caaaaaacag ccagaacggc acgctgcggg caatctccgc gacgcagacc
     7501 gtcatgggcg gcatctcctc cctaagctcc cttgcaaggt gttccaggcc cacttctgtg
     7561 agcatgcggc gcagacgcgc atacagctcg gacaccttca tgacggacaa atcgcactca
     7621 tgctccagat aataacgcac ggaagccagc aggtcttcct tgaacggaag ggttgttccg
     7681 gcacgccggg cagcgtgctc caaagcatcc gccagccatt gttccccgta ctcggtaatc
     7741 aggcaatcac caagcttgag caacggtctg ttgtttcgga agccgatcat ggaacaggaa
     7801 atttaaagaa tgggaagctt gcgctgcagg ggatcaataa gcgtctggtg gcgcatctgc
     7861 gtaatctcct ggataaactc ctccacattg gaaaactggc ggtacacgga cacatagcgg
     7921 atataagcca cgggatctat ggcatacaat ttttcaatca ccttcttgcc aatctcgctg
     7981 gaagggactt cgcgcaaatg gtcgcggtgc aattcggaaa tgatctcttc cacggcgcgg
     8041 tccaggcgat ccatgctgac gggccttttt tcacacgcct ttaccaggcc gcgtaaaatc
     8101 ttttcacgat tcagggcttc tctcagatta tcccttttga ctacgcgcaa ctccgtgcgt
     8161 tcaatctgct cataggtcgt ataacggtaa tcgcacctca aacacacgcg gcggcggcga
     8221 atggtcgtcc cgtctttgga catacgggag tcgatgacct tatcttccaa atgaccgcac
     8281 tgaacgcatc tcatggcgcg accatactac tacataacgc atccataaag taaaaagtac
     8341 gccaaatata gtacagtaga cttgatgatc aatgcttccc aagcagaact tcctgtgaat
     8401 aaaccggggt gaaaatgccg caggtcatgc ccctgttcgg tttccagacc gctccgctcc
     8461 tgtgaataag aaaaaagccc atggcccttc cgggaggaaa aaacgtattt ttttaacctc
     8521 ctccctccac taaaaaatac ggcaagcatt ccggaccgta ccaggtctat tgattacgga
     8581 ttcaccatgc cgccttgatt aacgacactg cccgcaacag gtctggagcc attcagattc
     8641 agcacagcgc cttgcttgac gaagactttg cccaaattca cggccttccc ctccataccc
     8701 ttcaaagtca tctccacctt tccggacaac gtccaggaac atttcagggc agagggcagc
     8761 acctgttcca atacgacgcg ggctgcttcc gccgtttctc cgccgccttc cgcttgggtt
     8821 acatgcacct ccgcctggcc ggacaaacgg acaggcatgg ctttttcctg aaccggcgag
     8881 ggcttaatct ccacggcaga accacccgga atattccagg actgccggcc cgccagctgt
     8941 acattcgtcc tcaactgcaa atttttcccc tccgccaggc gaatacctcc cggcccgatt
     9001 accaaacggt ctccggaact tcccttgctc cggacgataa cagtcttgac ggccggatcc
     9061 tccacacgga taccaaacaa cttcaccccc cctgacacca ggctgggttt tacggaagaa
     9121 gctacattcc acacgcatac gttcttcccc ggcattttcc ggttcatcca cgcgtccgga
     9181 gaacccaggg gaagatcgta acgaccgctt cgcacggagc cggtatcctt cccggccagc
     9241 cgggcggctt cataagcaaa acgggctcct atgctgatct gagactggcc gctgtaatgc
     9301 actcccattt gatctcctgc ggtcagtttg ggaagatctc gggtaggggc atagcccagg
     9361 gatggacggg attccgccag atccttcatc acctggcggg ttgtctttcc ctgaacttcc
     9421 gtatcgcgtc cttccaggcc gccggtaagg ttacccgttt ccggatcaga ttcattcttt
     9481 ccggcccaat tggcattttc tccaaggacg gctttctgcg cagccagtgc ggacgtatcc
     9541 ccgtacggtt tcagatctgc cgccagattg cccagaagtt ccagaaagcg ggaagcgctc
     9601 tccggaacgg aagttccggc attgctttcc ccctgcaaat acaaaagccc ggcaaactcc
     9661 actttggaat acttggacct gtccacaccc gcacaggcat tcttgacggc ctgcaccagc
     9721 gtcctgtaag cctggccgtt tttccgccaa tgggaattgt cgccgccgtc ccgggaagct
     9781 ttcacgaccg caacgtccgc atccttgaac catccattct tctccagagt aaaggcaaac
     9841 ccgtattccg gccccatgca taaattgccg ttataacgcg gcatagcagg ccttacctgc
     9901 tcccaggatg tggaagcgcc gggaaacacg ccttcccttt gcccaaaatt ttcatgccag
     9961 tacagagtct ccttcggttc atactttttt aacagctccg gagaagcagg gctgcccttc
    10021 accgccccca gagaattgga ttggcccgtc agcaaaaaaa tcttcaattc ttttgcagaa
    10081 cacacggaaa aaaagcacag gaaaaataaa aaggatagaa aacgaatcat ttgaattgca
    10141 tttgcctagg gacgactata gctgccggaa ttttcttttt caaccgtcaa ctattaccca
    10201 taggaacatg aacagggaga tgtaggaaaa gaccagcagt cttaaaaccc cattcaaaat
    10261 tttccgagaa ataatcagga aaaataaaaa ccggaagaat tattttctcc cggatttctt
    10321 caaacaattt tctgaataat aatgacgcag aggcgcatgg gcagtttctt tgcttccctt
    10381 tcccgcctct atcaggcttc ctgccttctg cgcctccgca tgaccagccc cgccaaaccc
    10441 agcaggctcc gggaagccgt cgccggttcc ggaaccaaat acgcagacat ggcagcctca
    10501 tgaagtatag caaccatttt ttccctacgg acaactcctt aactaaaaga atgtaggggg
    10561 agaagaacgg gaccgagcaa gaaaagaaca ggccccgcga gt
//
LOCUS       DAOMSU010000054        10552 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_379_length_10552_cov_3.131752, whole genome shotgun sequence.
ACCESSION   DAOMSU010000054 DAOMSU010000000
VERSION     DAOMSU010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10552)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_379_length_10552_cov_3.131752"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PK077_03790"
     CDS             complement(<1..321)
                     /locus_tag="PK077_03790"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor family protein"
                     /protein_id="HRN23450.1"
                     /translation="MEINVDIQPDCTATLKASIPAETTAARRASIVDSYAAKAKLPGF
                     RPGKTPKSIIEKRFKKEIEEELLDTLFETACSTALEENPKLKVLNFGKPEQSLDDQGN
                     YTATS"
     gene            689..3007
                     /gene="bamA"
                     /locus_tag="PK077_03795"
     CDS             689..3007
                     /gene="bamA"
                     /locus_tag="PK077_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HRN23451.1"
                     /translation="MTPEQVMRELGPGTGLLPGDQAYEGRPVKSVSVRYRSTGKTVSE
                     DRLKNLLATQPGTSYSPEVVNKDLERLLESGLVGGNTTVAVEPSGDGVAVVFEMAAQN
                     LLGGVGFRGNTAFDNRDLSEECGLKGGEALSDKALSSAIAKLRTYYQEARYPDVRVSY
                     FYQKTERPGFVDVVFDINEGKKANIIKIDFVGNEHISAKDLRQVMKTKEKGWLTWITK
                     SGRIDREQVEDDLAELVTYYRNKGYLRVKLDKVEYFDAGKGNEQKLTMRITLTEGRRY
                     KVNQVAFGPTKVFTAQELIPGLSMYNGDTYSAQKVADDVTMIRRYYGSRGYADASVRP
                     DIQEVGVDPKTGYGQINIVYHVTEGNPYRVGNIRLTGNNRTKDYVIRQELPLQSNDPM
                     NAVDLDTAQKRLQNLNYFDMVDVAQVGSTRPGYRDINIEVAEKRTGSLNIGVAFSSIE
                     SVYLFAGITQSNFDMYDWSSFVGGGQRFAINARVGFETQDASISWVDPWFLHRKLAFG
                     TEIFYSNSTYFSDYYDQQNYGFAISLRKPIGELDYVKLEYRLEQYRIDAEGNAPIFFW
                     QQDGDYLRSHVELSYTIDTRDAQIFPRKGGKFEVLAGYSGLGGDVHTYNFGVNGSYYW
                     NLRGDTIFSINAGAATVDSYGNHDVPIFERLYLGGPYNMRGFRFRDVAPYNPALSGDE
                     TMGGRSSFFCQFEYSIPVIEEVRVAVFYDIGFVNGDSFDFSTSKIVSDYGIGLRLNLP
                     IGPLAVDYAIPIQKNNAIDRGGQFQFYLNYSY"
     gene            3041..3784
                     /locus_tag="PK077_03800"
     CDS             3041..3784
                     /locus_tag="PK077_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapB"
                     /protein_id="HRN23452.1"
                     /translation="MSNFRILILAFLVVVLAVLGMIYVNISSRMDVIQQQQQMASAPA
                     PVNPSPYLPMPTQAPASAVQPVVVADPVVSEELAHVSAELERMKKENEELKKRHALAE
                     EERDLLERQNMEKGDPRLQWLNEIKDAEQVGRVTEYYRDANLVLFQSIGQPDLKVGQE
                     LGIRRRGSIFVSLIIDGVDPDGKVFQSYVKRNTLFDNNDKEPIKPGDEVIVPPASWQE
                     NMTEEDKSGMASPSAPVEAPKPVMIPMEP"
     gene            3821..4756
                     /gene="metF"
                     /locus_tag="PK077_03805"
     CDS             3821..4756
                     /gene="metF"
                     /locus_tag="PK077_03805"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HRN23453.1"
                     /translation="MHLRERLQSGAGPSLSFEFFPPKNEQAASALYQTIRELEPYRPS
                     FVSVTYGAGGSTRELTREVVLRIQHESRIPTVPHLTCVGHSREEVWNILDQYARAGVR
                     AVLALRGDPPRGAKDYNRDADVFRHAADLVTFIREYNESGRHPDPDGFAIGVAGFPEG
                     HPATPNRLREMDFLKAKVDAGADYICTQLFFDNHSFLDYRERCLLAGIKVPILAGIMP
                     VTSLSGMNRMAELSGGTCFPARLLKALKRAGSNPDAIQEVGIQYASSQCAELLDSDVD
                     GIHFYTLNQSRATREIYRNLGLKDSLQLLEHGEDK"
     gene            4809..5546
                     /locus_tag="PK077_03810"
     CDS             4809..5546
                     /locus_tag="PK077_03810"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN23454.1"
                     /translation="MDIKDIKRAMDNMPMGDGSAFAEISVGPAKHEQFLDEHYGKVAL
                     VVLLCVLGAAGWIIYNGVQDSMERKAGAALVAAMPESPATGEISLDEQGLQQVVVDFD
                     SSRAAVTASYLQAIALWNAGKEDEGVAKMKAFIDSAPTEEWKAQASVTLACRLMNRGK
                     ADEAEGLFRSVVDAGDPTFSGFATMCLGDIARAKKDNAAAGAFYRELAEKFPASAFAL
                     QSGGYLLRKSLFDIQSPVRIEPAVEQK"
     gene            5803..6075
                     /locus_tag="PK077_03815"
     CDS             5803..6075
                     /locus_tag="PK077_03815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23455.1"
                     /translation="MNRHIKSFWLLPLAGMTLSFMPSCTTDSYGNSSVTPGGAAAIGV
                     GALAAGAAIGAAVQHDRDKDRYRNDWRHHNPPPPPPRPPHRPQWRR"
     gene            complement(6168..6794)
                     /locus_tag="PK077_03820"
     CDS             complement(6168..6794)
                     /locus_tag="PK077_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HRN23456.1"
                     /translation="MPKRKSTREHLLNTGAMIVGESGLRALTVRGLSLRAGTNTGSFV
                     YHFGNREAFLTELLERWYEPLFTGIRTHANIHLPAFEKLEAILGDVMEFLLRHGQFIA
                     QLVLDALAGEQAAIRFISGIHTRHPLVLLETIREAQQEGSVLMGDPMNILIYLVCCGG
                     APFILSGQLQVHDPKAARPVLDLLGSILNDPESARQRMTWALRGIRRG"
     gene            complement(6927..10085)
                     /locus_tag="PK077_03825"
     CDS             complement(6927..10085)
                     /locus_tag="PK077_03825"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRN23457.1"
                     /translation="MNFLISFCLNNRITVILLTIALAIISIFVVKNIPVDVFPELKVP
                     RVTIQTEAPGLTAEEVEQYITIPIESAMNGTAGVKGVRSSSGSGLSFVWVDFDWNKDI
                     YQARQIVTERLGAVRESLPEGTSPELAPIVSITGEIMLIALTGDKDTSQLDMRQLAEY
                     KLRTRLLAIPGVGQVTVLGGRLPEYQVIYDPNKLKLAGVDLSSLKTAIQESQSSVPAG
                     YLEDVAGQELPIQQDTRTANIEQLNRALVPDHASGILRLQDVTEVKIDGAPRRGDAGF
                     MGEDAVVLSVQKVPGANTLALTQAVDAAVREFSQSQLPKGMKLHTAAYRQGDFIEMSL
                     DNGTETLLIAGAVVMIVIFLTLLNLRTAIITLISMPLSILFGMMMFPVFGLAINIMTL
                     GGLAVAVGDVVDNAIIFVEIAWRHLNRNAALPKDKRKSKYEVLMKAKSEIVGSISFSS
                     VIILLVFTPVLFLSGLEGQFFRPLGISYMLALLSSLIVAVTITPVLCYIWFKKSKNAA
                     TLESGDSFSSRLIKRIYAPILEFCLRFSKTVCAIMTAITLLALWLGSTFGTSFLPPFN
                     EDCYTVFVSTVPGTSLDETERISRKVMKDIEQIPGVLSVTQRTGRAENDEHAEPVSAS
                     ELLVRVDLKKDQKELRAAIKKCIDGIPGTSSMIGYPLAHRISSALSGSNSEIAINIYG
                     TELPQLRLAARKAKEILENMPEVADARANREIMVDTIRVQYNQEALASYGLTMANAAE
                     QVSTAMNGQKLGEVIKNQDHWNIVLRIDPRLKTSMEDVKNLELISPNKKTVRLDDVAQ
                     VYREEVSNLILRDNTMRKAMISCNPSPNSNLGDLAKACREQLDPVMNAMGCTVDYDGT
                     IKARESASQRLYVLGAIVMVLIVLLLSSALGSVRRAMLTLVNIPLCLVGGIVAVFLAS
                     PGTLSSLFGGTYIPPILSVASIVGFVTVIGFAIRSGLILLNRYRALEHQGMEPAEAIR
                     EGSLERVVPIIMTSLTTVLGLLPLIWAIDQPGGELLGPLAIVQFGGLVTATILNLLII
                     PATAKLFSRWIASRRKELKATS"
     gene            complement(10176..>10552)
                     /locus_tag="PK077_03830"
     CDS             complement(10176..>10552)
                     /locus_tag="PK077_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23458.1"
                     /translation="SRALYFTPDRIPEGTHAGQLARLDLYSHDAATEGFIPVPNSAVV
                     KVGVNDVVFIKAGNDTFIMKKVETLPSRQGKTPVKGLMPGQTIVTKGGYELKYILPTG
                     EAGSKKAAGHFHADGQFHEGEH"
BASE COUNT         2254 a         2750 c         3118 g         2430 t
ORIGIN      
        1 gctggtagcg gtgtagttgc cctggtcatc cagagattgt tccggctttc cgaagttgag
       61 cactttcaat ttggggtttt cttccagagc ggtggagcag gccgtttcaa aaagggtgtc
      121 cagcagttct tcttcaattt ctttcttgaa gcgcttttca atgatggact tgggtgtttt
      181 gccggggcgg aagccgggaa gcttggcttt ggcggcatag gaatctacaa tggaggcgcg
      241 gcgcgcagcc gtggtttctg cagggatgga agctttcagc gtggctgtgc agtccggttg
      301 aatatcaacg ttgatttcca taaataatac ggtcagtgtt aattgaaagt ggttctgttt
      361 cagctccggg atggcggcag cggtcaaaac acgagcggcg ataactttaa tgagagggta
      421 tctggagtgc aagctattgc acaggaatga cacaattgcc ctcctaagcg cgcacgcgca
      481 cgcgatgtaa tagaatacgg ggcaactgtg ttctacgcgg aatcacgtct tgactatgca
      541 agaggaaaag tcctttatat cccaagcatt aagttcagcg ctcatgattc gtcccgtttt
      601 tttgtgtgtt tcctttccct tgatagcatg ttcggctgtt ttggcccagg atgaaacttc
      661 ccttaacaga ggcgcctatg gttccgtgat gactccggaa caggtaatgc gggaactggg
      721 gccgggcacg gggcttcttc ccggtgacca ggcgtatgag gggcggcctg tgaagagcgt
      781 ttccgtgcgg tacagaagca ccggcaagac cgtgtccgaa gaccgtctga aaaatttgct
      841 ggctacgcag ccgggcacca gttattcccc ggaagtggtc aacaaggact tggaacggtt
      901 gttggaaagc ggtctggtag gcggcaatac aacagtagcc gtggagcctt ccggcgacgg
      961 cgtggcggtg gtgtttgaaa tggcggccca gaatctgctg ggcggcgtgg ggttccgcgg
     1021 caataccgcg ttcgacaaca gggacctctc cgaggaatgc gggctgaagg ggggggaagc
     1081 tctcagcgac aaggccctga gcagcgctat tgccaagttg cgcacttatt accaggaagc
     1141 tcggtatccg gatgtacgtg tttcctattt ttaccagaaa acggaacggc ccggctttgt
     1201 ggatgtggtc ttcgacatta acgaaggaaa aaaggccaat attatcaaga ttgactttgt
     1261 gggcaatgaa cacatcagcg ccaaggatct ccgacaggtg atgaaaacca aggagaaggg
     1321 ctggttgacc tggattacca aatccggacg cattgaccgt gaacaagtgg aggacgatct
     1381 ggcggaattg gtgacttatt accgcaataa aggttatctg cgcgtcaagc tggacaaggt
     1441 ggaatatttt gacgccggca aaggcaatga gcagaagctg acgatgagaa ttacccttac
     1501 cgaggggcgc cgctacaagg tcaaccaggt tgccttcggc cccaccaagg tgtttacggc
     1561 ccaggaactg attcctggcc tgagcatgta caacggggat acctattccg cccagaaagt
     1621 ggccgacgac gtgacgatga tccgccgtta ttacggttcc cgggggtatg cggacgcctc
     1681 cgtgcgtccg gacattcagg aagtgggtgt tgatcccaag acaggttacg gacaaatcaa
     1741 catcgtgtac catgtgacgg aaggcaatcc ctaccgcgta ggcaatatcc gcctgaccgg
     1801 gaataaccgc acgaaggatt acgtcatccg ccaggaactt cccctccagt ccaatgaccc
     1861 catgaacgcc gtggatttgg ataccgccca gaagcgcctc cagaacctga actattttga
     1921 catggtggac gtagcccagg tgggatccac gcggcccggc taccgtgata tcaatatcga
     1981 ggtggcggaa aaacgtacgg ggtccctgaa tatcggcgtg gcattttcct ctattgaaag
     2041 cgtttacctg ttcgcaggca tcacccagtc caattttgac atgtatgact ggtcctcttt
     2101 cgtgggcggc ggccagcgtt tcgcgattaa cgcccgtgtc ggttttgaaa cgcaggatgc
     2161 cagtatttcc tgggtggatc cgtggttcct gcaccgcaag ctggccttcg ggacggaaat
     2221 cttctacagc aattccacat atttttccga ttattacgac cagcagaact atggctttgc
     2281 catttctctc cgcaagccga tcggggaact ggattacgtg aaactggaat accgcctgga
     2341 acagtaccgt attgacgccg aagggaacgc ccctatcttc ttctggcaac aggacggaga
     2401 ctacctgcgc agccatgtgg aactttccta caccattgat acccgcgacg cgcagatttt
     2461 tccccgcaag ggaggcaaat ttgaagtgct ggccggttat tccggcctgg gcggcgatgt
     2521 gcatacatac aatttcggcg tgaatggctc ctattactgg aatttgcgcg gagacaccat
     2581 tttcagcatc aatgccggtg cggctaccgt ggattcctat ggcaaccacg atgtgcccat
     2641 ttttgagcgc ctctacctcg gcgggccata caacatgcgc ggtttccgtt tccgggacgt
     2701 ggcgccgtac aatcccgccc tgagcggtga tgagacgatg ggcggccgtt cctccttctt
     2761 ctgccagttt gaatattcca ttcctgtgat tgaagaagtg cgcgtcgcgg tgttttatga
     2821 catcggtttt gtcaacggag attcttttga cttcagcacg tccaaaatag tttccgacta
     2881 cgggatcggc ctgcgcctga acctgcccat cggtccgctg gcagtggact atgccattcc
     2941 gattcagaag aacaacgcca ttgaccgcgg aggacaattc cagttctatc tcaattattc
     3001 ctattaatta cgtttgataa tttctctacc gtttgacgac atgagtaatt tccgcattct
     3061 tatcctggcg tttctggtgg tggtgctggc tgttctggga atgatttatg tgaatatttc
     3121 ttcaagaatg gatgtgattc agcaacagca gcagatggcc tccgctccgg cacctgtcaa
     3181 tccctccccg tatcttccga tgcccacgca ggctcctgct tccgccgttc agccggtggt
     3241 ggtggccgat cctgtagtga gcgaggaact ggctcatgtt tcggcggagc tggaacggat
     3301 gaagaaggag aatgaagaac tgaagaagcg ccatgctctg gcggaggaag aacgcgattt
     3361 gttggaacgc cagaatatgg aaaagggaga tccccggctg caatggctta atgaaatcaa
     3421 ggatgcggag caggtgggcc gtgtgacgga atactaccgt gacgccaacc tggtcctgtt
     3481 ccagtccatc ggccagcctg acttgaaagt ggggcaggaa ctgggaatcc gcagacgcgg
     3541 gagcattttc gtatccctga ttatcgacgg agttgacccg gacgggaagg tgttccagtc
     3601 ttacgtgaag cgtaatacgc tttttgataa taatgacaag gaacccatca agccggggga
     3661 tgaagtcatt gttccgcctg cgagctggca ggaaaacatg acggaagaag ataagtccgg
     3721 tatggcttct ccctccgccc ccgtggaagc tcccaaaccg gtgatgattc ccatggagcc
     3781 gtgatgcggg tccgtattta gccaagccaa gtaaagagaa gtgcacctga gagaaagatt
     3841 gcaatcgggc gccgggccca gcctgtcctt tgaatttttc ccgcccaaaa acgagcaggc
     3901 agcgtcggcc ttgtaccaga ctatccgtga actggagcct taccgcccca gtttcgtcag
     3961 cgtgacgtat ggcgcagggg gctccacccg cgagcttacc cgcgaggtgg tgttgcgcat
     4021 ccagcatgaa tcccgtattc ctacggttcc ccaccttact tgcgtagggc atagccggga
     4081 ggaggtatgg aatatcctgg accaatacgc ccgggctgga gtgcgggccg ttcttgcact
     4141 tcggggagat cctccccggg gagcgaagga ttacaaccgc gatgcggatg tttttcgcca
     4201 tgcggcggat ctggtgacgt ttatccgtga atataatgaa tccggccgtc acccggatcc
     4261 ggatggattc gccataggcg tggccggctt cccggaaggt catccggcca ctcccaaccg
     4321 cctgcgcgag atggatttcc tgaaagccaa ggtggatgcc ggtgcggatt atatctgcac
     4381 ccagctgttt tttgacaatc attcttttct ggattaccgg gaacgctgtc tgctggccgg
     4441 catcaaggtg cctatcctgg cgggaatcat gcctgtgacc agcctgtccg gcatgaaccg
     4501 catggcagag ctttccggag gcacctgttt tccggccagg ctgctcaagg cgctgaagcg
     4561 tgcgggttcc aatccggacg ctattcagga ggtaggcatc caatacgcct ccagccagtg
     4621 cgccgaactg ttggattccg atgtggacgg cattcatttt tatacgctta accagagccg
     4681 ggcaacgcgg gagatctaca ggaatctggg actgaaggat tccctgcagc ttttggaaca
     4741 tggcgaagat aagtaaaaag ctgattgatt tggttgggac aacacggtag aaacctcttc
     4801 aacatattat ggatatcaag gatattaaac gggccatgga caatatgccg atgggcgacg
     4861 gaagtgcgtt tgcggaaatt tccgtcggcc cggccaagca tgaacaattt ttggatgaac
     4921 attacggcaa ggttgcgctg gttgtgttgc tatgcgtcct tggggctgcc ggatggatta
     4981 tttataatgg cgtgcaggat tccatggaaa gaaaggcagg cgccgctctg gtggctgcta
     5041 tgccggaatc cccggcaacc ggggaaattt ccctggacga acaggggctt cagcaggtag
     5101 tggttgactt tgacagttcc cgcgccgccg tcaccgcctc ttaccttcag gccattgcct
     5161 tgtggaacgc cggcaaggag gatgaaggcg tggccaaaat gaaagctttt attgattccg
     5221 ctcccacgga ggaatggaaa gcccaggctt ccgttaccct ggcctgccgt ctgatgaacc
     5281 gggggaaagc ggatgaggcg gaagggttgt tccgttccgt ggtggatgcg ggagatccta
     5341 ctttttccgg ttttgccacc atgtgcctgg gggacattgc ccgggctaag aaagacaatg
     5401 ccgccgcagg cgccttttac cgtgaactgg cggagaaatt tccggccagt gcatttgcct
     5461 tgcagagcgg aggctacctg ctgcgcaaga gcctgtttga tattcagagt cccgttcgta
     5521 ttgagcccgc agtagaacag aaataatcca tgcgggcgtt tccgaagttt tccggaagat
     5581 gaacgctttt tccatccgca tgtctgtttt tttacagtgc atgcggattt ccgtattcag
     5641 gaacgccgga acaggggggt aatgaaaagc ctgctgcgcg cgtagctgtt atgattccgg
     5701 gaattccgtt caggactgtg acatatttaa acaaatcatc aagggattcc gcgcttaccg
     5761 gatgtaaccg ggctgccggt gttaatcctt attttttatc tcatgaacag gcatattaaa
     5821 tcattctggc ttcttcccct ggcgggaatg acgctttctt tcatgccgtc ctgcacgacg
     5881 gacagctacg ggaacagttc cgtaacgccc ggcggagccg ccgccatcgg tgtgggggcg
     5941 ctggctgccg gcgctgctat tggggcggct gtccagcatg accgtgacaa ggacaggtac
     6001 cgcaatgact ggcgccacca taatcctcct cctccgcctc cccgtccgcc tcatcgaccc
     6061 caatggcgga ggtaagaagg gagcgtgcgt gtaagggcgg cgatattttg gttcggcagg
     6121 gggccgggag gcgacgcgtt tttccggtct gcccctttgc tgaaatctca gccccgccgt
     6181 atgccgcgga gcgcccaggt catgcgttgt ctggcgcttt ccggatcatt gaggatagaa
     6241 cccagcaagt cgagaacggg ccgagctgct ttggggtcgt gcacttggag ctgtccggaa
     6301 aggataaagg gggctccccc gcagcagaca aggtaaatga ggatgttcat ggggtctccc
     6361 atgagcacgg atccttcctg ctgggcttcc cgtatggttt ccagcagcac aaggggatgg
     6421 cgcgtatgga ttcctgaaat gaaacggatg gccgcctgtt cccctgccag ggcgtccaga
     6481 accagctggg caatgaattg gccgtgacgc agcaggaact ccatgacgtc tcccaggatg
     6541 gcttccagtt tttcaaaggc cggaagatga atgttggcat gggtcctgat gccggtgaac
     6601 agaggttcgt accagcgttc cagaagctcg gtcagaaaag cttcccggtt gccgaagtga
     6661 tagacaaagc tccctgtatt ggtgccggcc cgcagggaca ggccgcgaac ggtaagggcg
     6721 cgcagcccgg attccccgac aatcatggca cccgtattga gcaggtgctc tctggtggat
     6781 ttccgtttgg gcatgaaggt gaagcttgaa cctccatgtg tgccccttat cggcctgcaa
     6841 ggcaaggact cgttatgtct gttgctgtaa aaagcggaac cggggctcat cctctccccg
     6901 gtcccgccgc cggagtcctg aatgaatcag gaggttgctt tcaattcctt ccgccgggag
     6961 gcaatccagc gggagaagag tttggccgtg gccggaatga taagcaggtt caggatggtg
     7021 gctgtcacca gcccgccgaa ctggacgatg gccagggggc cgagcagttc gcctccgggc
     7081 tggtcaatgg cccagatcag cgggagcagc cccagcacgg tagtcaggga agtcatgatg
     7141 atggggacca cgcgttccag agaaccctcc cgtatggctt ccgcgggttc catcccctga
     7201 tgttccaggg cccggtacct gttgagcaga atcaggccgc tgcggatggc gaagccgatg
     7261 acggtcacga accccacaat ggaagccacg gaaagaatgg gaggaatgta agttcctcca
     7321 aacagggagg acagcgtacc gggggaggca aggaaaacgg ctacgatgcc gcccaccagg
     7381 cacagcggta tgttcaccag agtcagcatg gcgcgccgga cgcttcccag tgcggaggaa
     7441 aggagcagca cgatgagcac catgacgatg gcgcccagga cgtaaagccg ttgtgatgcg
     7501 gattcccgtg ctttgatagt gccgtcatag tctacggtac agcccatggc gttcattacc
     7561 gggtccaatt gttcccgaca ggctttcgcg aggtctccca ggttggaatt gggggaggga
     7621 ttgcaggaaa tcatggcttt ccgcatggtg ttgtccctca ggatgaggtt ggaaacttcc
     7681 tcccggtaca cctgtgccac gtcgtccagg cgcacggttt ttttattggg ggaaatgagt
     7741 tccaggtttt tgacatcttc catggacgtt ttaagtctgg ggtcaatgcg caggacaatg
     7801 ttccaatggt cctggttttt gatgacttct cccagtttct gtccgttcat agccgtggaa
     7861 acctgttccg cagcattcgc catggtcagg ccgtaggaag ccagggcttc ctgattgtat
     7921 tgcacgcgaa tggtatccac cataatttcc cggttggcgc gtgcatccgc cacttccggc
     7981 atgttctcca gaatttcctt ggctttccgg gccgccaggc ggagctgggg cagctccgtg
     8041 ccgtaaatgt tgatggcgat ttccgagttg gagccggaca gagcggaact gatgcgatgg
     8101 gccagcggat agccgatcat ggagctggtg ccggggatgc cgtcaatgca tttcttgatg
     8161 gcggcgcgca attctttctg gtctttcttc agatccacgc gcacgagcag ctcggaggcg
     8221 ctgacgggtt ctgcgtgttc gtcgttttcc gcgcgtcccg tgcgctgggt gacactgaga
     8281 acccccggaa tctgttcaat atctttcatc actttccggg agatgcgctc cgtttcatcc
     8341 agggaagttc ccggcaccgt gctgacgaag actgtgtagc aatcctcgtt gaacggcggc
     8401 aggaaactgg ttccgaacgt ggaacccagc cacagggcca gcagcgtaat ggccgtcatg
     8461 atggcgcaga ccgttttgga aaagcgcagg cagaattcca ggatgggggc ataaatgcgt
     8521 ttaatgaggc gggagctgaa ggaatccccg ctttccagag tagcggcgtt tttgcttttc
     8581 ttgaaccata tgtaacagag aaccggagta atggtgacag ctacaataag agaagaaagc
     8641 agggccagca tgtaggagat ccccaggggc cggaaaaact ggccttccag accggacagg
     8701 aagagcaccg gagtgaatac cagcaggatg atgacggagg agaaggaaat ggagcctacg
     8761 atttcgctct tggctttcat cagaacctca tacttgctct tgcgcttatc tttcggcagg
     8821 gcggcgttcc tgttcaggtg cctccaggct atttccacga agataatggc gttgtccacc
     8881 acgtctccca cggctacggc aaggccgccc agggtcatga tgttgatggc cagcccgaag
     8941 acggggaaca tcatcatccc gaacaggatg gacaggggca tggaaatcag cgtgatgatg
     9001 gccgtgcgaa ggttcagcag cgtcaggaag atgacgatca tgaccaccgc tccggcaatg
     9061 aggagggttt ccgtgccgtt atccagggac atttcaataa aatccccctg ccgataggct
     9121 gcggtgtgca gtttcattcc cttgggaagc tggctttgac tgaattccct gacggctgca
     9181 tccacggcct gtgtcagggc cagagtattg gctcccggca ccttctgcac ggaaagcact
     9241 acggcgtctt cccccataaa tccggcatct ccccgccgcg gcgcaccgtc tatcttgact
     9301 tccgtcacat cctggagccg gagtattccg gaggcatggt ccggaaccag ggcgcggttg
     9361 agctgttcaa tattggcggt gcgggtatcc tgctggatgg ggagttcctg cccggctaca
     9421 tcctccagat accctgcggg gacggaagat tgggattcct gaatagccgt tttcaggctg
     9481 gaaaggtcca ccccggccag tttgagtttg ttgggatcat aaatcacctg gtattccggc
     9541 agacgcccgc ccagcaccgt gacctgcccc accccgggaa tggccagcag gcgcgtgcgc
     9601 agcttgtatt ccgccagttg cctcatgtcc agctgggagg tatccttgtc tccagtcagg
     9661 gcgatgagca tgatttcccc cgttatggag acgatggggg ccagctccgg ggatgtccct
     9721 tccggcaggg attcccggac tgctccaaga cgttccgtta caatctgccg ggcctggtag
     9781 atatctttgt tccagtcgaa atccacccat acgaaggaaa gcccgctgcc ggaggaggag
     9841 cggacgcctt tgactccggc ggttccgttc atggcggact caatgggaat ggtgatgtat
     9901 tgttccactt cttccgcagt aaggccgggg gcttccgtct ggatggtgac acgcgggacc
     9961 ttcagttccg ggaatacgtc cacgggaatg ttcttcacca cgaaaatgct gatgatggcc
    10021 agcgcaatcg tcagcaggat cacagtgatc ctgttgttca ggcagaatga aatgagaaag
    10081 ttcatggatt ctgattgctg aatacctaag attcaaaggt ttattccaag gaaatccggg
    10141 atatttgttc cttaatcttt tattttgtac atcgtttagt gttctccttc gtggaactgg
    10201 ccgtccgcgt ggaagtgccc ggctgctttt ttgcttccgg cctctccggt gggcaggatg
    10261 tatttcaatt catacccgcc cttggtgacg atggtctgtc ccggcatcaa ccccttgacg
    10321 ggcgtttttc cctgccggga aggcagtgtt tccacttttt tcatgataaa tgtatcgttt
    10381 cccgccttga tgaaaaccac gtcgttcacc cctaccttga cgacggcgct gttggggacg
    10441 ggaatgaagc cctccgtggc ggcgtcatgg gagtacagat ccaaccgggc cagctggccg
    10501 gcatgtgtcc cttccggtat gcggtccggg gtaaaataca gggccctgga ct
//
LOCUS       DAOMSU010000055        10086 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_413_length_10086_cov_2.882564, whole genome shotgun sequence.
ACCESSION   DAOMSU010000055 DAOMSU010000000
VERSION     DAOMSU010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_413_length_10086_cov_2.882564"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>839
                     /locus_tag="PK077_03835"
     CDS             <1..>839
                     /locus_tag="PK077_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRN23459.1"
                     /translation="EDMHQLEAVGGSAGLAEIYTFTTTGAYFEHYLNVLKDKFILRSI
                     IDIANQSTSQAFDNPDDVAELLDSVETHIFQIRERYNSAKDEQSLASILKQAVTNFEK
                     FIASKGQIQGLTTGFEELDKKSNGLKPGDMFIIAARPSMGKTSFLLNIIEHIALNEKK
                     PTLLFSCEMPAVQIVERLLFARSGVRSREIIKRGNLTQLEMKHFKQAVKEVGASQLVI
                     DDTAAISINELRAKARRVMRDQGGLAAIGVDYLQLMRSHSKQAANSREREVAEISAGL
                     KAL"
     assembly_gap    840..849
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <850..>1168
                     /locus_tag="PK077_03840"
     CDS             <850..>1168
                     /locus_tag="PK077_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRN23460.1"
                     /translation="KELKVPVIVLAQLNRGPESRTGASLGVPRISDLRESGSIEQDAD
                     MIGLLYRSAYYAEDEEKRQQMAGRANLHLAKNRNGPTGDVPLHFEAELMRFSTREADE
                     HDQE"
     assembly_gap    1169..1180
                     /estimated_length=12
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1624..2385
                     /locus_tag="PK077_03845"
     CDS             1624..2385
                     /locus_tag="PK077_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN23461.1"
                     /translation="MVTEWIGNAGPTEGNTYELGNADNWSNGVPARGNNQGPDVIFNN
                     TGTITLSGSMVDTSDGGSITVTGNSNVTVGGTRWTGNVTIGAGSALSLSQVDFKSSDI
                     ILDGTFNLGVCGIDSGGNGARLVFGIGGIMNVNQKIWGASDFSVSGTLATTSTDLAAG
                     EFQFVTRTLITSAGFDGGSISLGDFTAEDGGALTKASGIMEGNAADYQGQYYLYTENG
                     NVKVQYVVAGAVPEPATATLSLLGLASLMLRRRRA"
     gene            2539..3159
                     /locus_tag="PK077_03850"
     CDS             2539..3159
                     /locus_tag="PK077_03850"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HRN23462.1"
                     /translation="MQTHAKSLNERFRTLISLIFGLAIGCSLLFGESRWETSPLIEES
                     LMLLACFMAGIGAFGRIWCSLYIAGYKNNVLVTDGPYSMCRNPLYFFSFVGSIGVSCA
                     TETFTIPLLTALAFGIYYPSVIRKEQERLTALFGDAYRNYCRNVPSFIPSLSRLKPPP
                     AAYSVNPATFTHNIVDALWFIWFIGIFEFISGLHEAGMLPVWFLIP"
     gene            complement(3149..4393)
                     /locus_tag="PK077_03855"
     CDS             complement(3149..4393)
                     /locus_tag="PK077_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HRN23463.1"
                     /translation="MKQIRVELINTGTEILLGSIVNTNAAWLGNRLFEAGFRVGRVTV
                     VPDGYAINEAMRESARRADIVIVSGGLGPTSDDVTREALCDVCGVDMHRDEHVAEWLK
                     SYFKRRGIPIAECNFKQAMVPDGAAVLENSNGTAPGLVMPASGSLPMFILLPGPPAEL
                     KPMVEHSVMPLLEDLADEDISHLRVFRLVGIGESDLQELVDDSLHQVEGLEVAYCARV
                     GEVDVRLVGSEVALKQGEARLRILAGAYVLAPVGASLEKAVVCHLAQQGLKAATAESC
                     TGGLIAKRITDVPGASGVFECGWVTYADRAKTEMLGVPEEVLKRCGAVSEPVVKAMAE
                     GALERSGADVAVAVSGIAGPGGGTPEKPVGTVWLAWAFRGGKTRTEMMLYPRDRASFR
                     KMVSQRALAGLLDARRKEPVTE"
     gene            4506..5927
                     /gene="pyk"
                     /locus_tag="PK077_03860"
     CDS             4506..5927
                     /gene="pyk"
                     /locus_tag="PK077_03860"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRN23464.1"
                     /translation="MNTPSRPRATKIICTIGPATDTSDMLGQLIEAGANVFRLNMSHS
                     KHDWVREVVFRIRRKAAELKANVAILFDLQGPSIRTGDLMEPYHLKKGDTVEIRLGTA
                     KPELPFSTTVNYDGLLQDVQAGHTMVVDNGGILMRIAEVRPDRLICDVLTEGVFGSRR
                     HINLPGVALRLPALTEKDLADLAVAVECETDYVAMSFVRDAAHIAQLREHIDNLGGKA
                     QIVAKIEDQQAVRHIDDIILAADVIMVARGDLGIEVSIEELPIIQRRIVRHCHRLGRR
                     CIVATHMLESMITQPTPTRAEVTDVSNAIFEQVDAVMLSGETSVGHYPVRCVEVLDSI
                     AHRMECSGNLNFAASAILNGDRQKATKAAISLADSVENACLIVFTRRGLAATQAAVLR
                     PERAAIFAFSNDPVVVRQLALARDVTAFESPFLKDPARMISTALDLLKEKGLISSGQP
                     VVIQGDSLQGELLADSIIFMRVE"
     gene            5963..6442
                     /locus_tag="PK077_03865"
     CDS             5963..6442
                     /locus_tag="PK077_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN23465.1"
                     /translation="MWPPPAPLRELLLDADPDERRIADHLAHGELYVAKNCGTAAGVI
                     VIRQTREHTWEIMNCSVAPEYRRQHCGTALVRHALDIIRNKGGRYAEVGTSDASPGPM
                     ALYESCGFQISGVINNHFTDNYPEPVWDNGVQCIDMIRMRADLSLRKEGAHHAAQKA"
     gene            complement(6430..7257)
                     /locus_tag="PK077_03870"
     CDS             complement(6430..7257)
                     /locus_tag="PK077_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23466.1"
                     /translation="MNFFFSFLFGAGIVSVASMAVSSAESRIPEVPSAPALRPVSGSI
                     KAGYSTNYEFRGLIPAGCNPTAPLRLDWRSDLNDRYSLILALKEEIFLGHPAVDIENE
                     TMVDLGLQRKFGKATYAALSFRANDGGLAGFASERLFGNGGTTYEAALIMRHDVGFLP
                     GFYAQGSAAYSFYGITGWWFDMCTGSDFRMTENLYMGFKFGAVLSSSYWPSSSNGWQS
                     LYFRVTANYRLFGNVFIEPFVGLHWLGKGSHGVNRVYGRTLVKGNSWVTGVSVVYAF"
     gene            complement(7380..7997)
                     /locus_tag="PK077_03875"
     CDS             complement(7380..7997)
                     /locus_tag="PK077_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23467.1"
                     /translation="MAGERDFSFHNETDFLFGWKNRDGLLKNLSTTLGWNLIHGGLLG
                     NFARYDYNATWMNDEDVYIRNTHSVIQEFYLTLDYGLPGNWFAGVTTSYAFQGMTGWW
                     FRPHVGYQAAICSDIDFVLSAGMSATSSYFDNKSLFMSNGSQAWWLKAELPVKLGAKN
                     LSLVPFVSFNWAGEGALKANRGSDRGDKPYKNFGVVAGAGLVYSF"
     gene            complement(8450..9334)
                     /locus_tag="PK077_03880"
     CDS             complement(8450..9334)
                     /locus_tag="PK077_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23468.1"
                     /translation="MQTKNIISVAAVVASFAGAALAGTPVKIVAPTPVPAPVATSSPL
                     SGDIYAGYASNYTCRGIVASHALAEGDSVIPAGVNLNYKLCDANSIVASASYTTLTSG
                     HHLMGDRDTSFHNETNFNLGWQNKDGLLKNLSTTLGWNLIHGGLLGSFAKYDHAHSVT
                     QEFYLNLNYDLTQNWFAGVTTSYAFQGMTGWWFQPYVGYKAALCPVTDIVVTAGMSAT
                     SSYFGAAFEQANGAQAWWVKAELPVKLGVKNLSLVPFVSFNWAGCGALKVNKGLDKGD
                     KPYKNFGVVAGASLVYSF"
     gene            complement(9460..>10086)
                     /locus_tag="PK077_03885"
     CDS             complement(9460..>10086)
                     /locus_tag="PK077_03885"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRN23469.1"
                     /translation="ACIPLYILRIWDENRMKRAYLMYLWGLPAETVREYGRKFAEMAQ
                     EWIYPELKERLAEYRKKGYLCLMVSASPSFYVKPLGELLGFDEVLGTDVLLEERMPAM
                     PELPNGNNKGAVKVERLRERNVLPEHGVLENAVAYSDSAADLPMLLSCRRRVLVNPSP
                     ALKEDRLLDGAECLYPARPWKGRFGKIWRIGFFVMGLVDVNNVKSMTC"
BASE COUNT         2468 a         3103 c         2425 g         2068 t
ORIGIN      
        1 tggaagacat gcaccagctg gaagccgtag gcggaagcgc gggcctggcg gaaatataca
       61 cctttaccac caccggagcc tattttgagc attacctcaa tgtcctgaag gacaaattca
      121 tcctccgctc catcattgac atcgccaacc agtccacctc gcaggccttt gacaatccgg
      181 acgacgtagc ggaactgctg gattctgtgg aaacccatat tttccagata cgggaacgct
      241 ataacagcgc caaggatgaa caaagcctgg ccagcatcct taaacaggcc gtcaccaatt
      301 ttgaaaaatt tatcgcaagc aaaggccaaa tccagggact gaccacggga ttcgaggaac
      361 tggacaaaaa aagcaacggg cttaaaccgg gggacatgtt catcatcgcc gcccgtccat
      421 ccatggggaa aacctccttc ctgctcaaca tcattgagca catcgcgctg aatgagaaaa
      481 aacctactct gctcttttcc tgtgaaatgc ccgcggtcca gattgtggaa cgccttcttt
      541 tcgcccgttc cggagtccgc agccgggaaa tcatcaagag aggcaacctc acccagcttg
      601 aaatgaagca cttcaaacag gccgtcaagg aagtgggggc ttcccagctt gtcattgacg
      661 ataccgcagc catctccatc aacgaactcc gggccaaagc gcgccgggtc atgcgggacc
      721 agggaggttt ggccgccatc ggcgtggact atcttcagct catgcgctcc cactccaaac
      781 aggcggccaa cagccgtgaa cgagaagtgg cggaaatatc cgccggcctc aaagccctgn
      841 nnnnnnnnnc aaggaactca aagtgcctgt catcgttctg gcccagctca accgcggtcc
      901 ggaaagccgc acgggagcca gcctgggcgt cccccgcatc tccgaccttc gcgaatccgg
      961 ctccatcgaa caggacgcgg acatgatcgg cctgctctac cgctccgcct actatgcgga
     1021 agatgaggaa aaacgccagc agatggccgg gcgggccaat ctgcatctgg ccaaaaaccg
     1081 caacggcccc acgggggacg ttccgctcca cttcgaagcg gaactcatgc gcttctccac
     1141 ccgcgaagcg gatgaacacg accaggaann nnnnnnnnnn ccagacgcct gttctctacg
     1201 gattagcaat ccggggtcaa gctttgattc taagcgggaa taacttgtat tggcgtttcc
     1261 ttccatgcca agaggcgttt ttatgaaatg aagtctgccc ggcgccattg gcttccaatg
     1321 aaagccctga gatcctttgc gttatttaaa tctgtagtta aattttgata actctctttt
     1381 ctggaatacg ttacatgtaa gaagaagtct gtatttcaat ggctgtagct ctttgcaact
     1441 tttcctaagt caaacaggat tttcttcggt ttctgcctat aacggattgc taacccggta
     1501 gcatcacggt agcaccagcg tttcctctgg gcttattaat ctagttattt ttttaatatg
     1561 aaaaaaaact tatttattac tacaatcctc tgcggatcat gcatctgtgc ccatgccgcc
     1621 agcatggtca cggaatggat cggcaatgcc ggccctacgg aaggcaacac ctatgaactg
     1681 ggcaatgcgg acaactggag caacggtgta cccgcccgcg gcaacaacca ggggccggac
     1741 gtcatcttca ataacaccgg aaccatcacc ctcagcggct ccatggtgga cacatccgat
     1801 ggcggcagca tcaccgtcac gggcaacagc aatgttacgg ttgggggaac ccgctggacg
     1861 ggaaatgtca ccattggagc aggttccgct ctttctctct cacaagtgga ctttaaaagc
     1921 agtgacatca tcctggacgg caccttcaac cttggtgtct gcgggattga ttccggcggc
     1981 aacggcgccc gccttgtctt cggcattgga ggcatcatga acgtcaacca aaaaatatgg
     2041 ggagcttcgg acttctccgt ttccggtacg ctggccacca catccacgga tctggctgct
     2101 ggagaattcc agtttgttac ccgcacgctg attacctccg ccggctttga cggcggttcc
     2161 atttccctgg gagacttcac cgcggaagac ggcggcgcgc tgaccaaggc gtccggaatt
     2221 atggaaggga acgccgcaga ctaccagggc cagtactatc tctacacgga aaacggaaac
     2281 gtcaaggtgc agtacgtagt ggcgggagca gtgccggaac ctgctacggc caccctcagc
     2341 ctgctggggc tggcttccct gatgctgcgc cgcaggcgcg cctgaccttc cacaccctgt
     2401 cttttttcct caacagcctg tatgcccacg catacaggct gttttatttt caggcgagtg
     2461 acgaaaacgt cacaaaaaac atcttcatgc cattgccggg aatccgtagc atttttccat
     2521 tatctcccgt tcgacgccat gcaaacccac gcaaaatcac tcaacgaacg tttccgcaca
     2581 ctcatctctc tgattttcgg tctggctatc ggctgttccc tgctcttcgg ggagtcccgc
     2641 tgggaaacgt ctccgctcat tgaagaaagc ctcatgctcc tggcctgctt catggccggc
     2701 ataggcgcct tcgggcgcat ctggtgttcc ctctacattg cgggctataa aaacaacgtc
     2761 ctggttactg acgggccata ctccatgtgc cgcaacccgc tctatttctt cagcttcgtt
     2821 ggcagcatag gcgtatcctg cgctacggag accttcacca tccccctgct tacggcgctg
     2881 gcgttcggaa tctactaccc gtccgtcatc agaaaggaac aggaaaggct caccgccctt
     2941 ttcggagacg cctaccggaa ctattgcaga aacgttccct ccttcattcc ctccctctca
     3001 cggcttaaac cgccgcctgc cgcctactcc gtcaatcccg ccacattcac gcacaacatt
     3061 gtggatgcgc tgtggttcat ctggttcatc ggcatctttg aattcatcag cggacttcat
     3121 gaagccggaa tgcttcccgt gtggttcctc attccgtaac aggctctttt ctcctggcat
     3181 ccagaagccc cgcaagcgcc cgttgggaaa ccatcttacg gaaagatgcg cggtcccgcg
     3241 gataaagcat catctccgtc cgcgtcttcc ccccccggaa agcccaggca agccatacgg
     3301 tccccacagg cttttccggc gtaccgcccc cgggtcccgc aataccgctg acggcgacag
     3361 cgacatcggc tccggagcgt tccagggctc cttccgccat cgctttaacc acgggctcgc
     3421 tcacagcccc gcaacgcttc aggacctcct ccggcacgcc cagcatctcc gtctttgccc
     3481 ggtcggcata agtaacccac ccgcattcaa aaaccccgga ggcccccggc acatccgtaa
     3541 tcctcttggc aatcaggccg ccggtgcagc tctccgccgt cgccgccttc agaccctgct
     3601 gcgccagatg gcacacaacc gctttctcca gggatgcccc cacaggagcc agcacatacg
     3661 ccccggccag aatgcgcaaa cgggcctctc cctgtttcaa agccacctca ctgcccacca
     3721 ggcgcacgtc cacttccccg acgcgcgcgc aatacgccac ctccagaccc tccacctggt
     3781 gcagggaatc atccaccagc tcttggagat cgctctcccc tatccctacc aggcggaaca
     3841 cgcgcaaatg agaaatatcc tcatccgcca aatcttccag aagcggcatc acggaatgtt
     3901 ccaccatcgg cttcaactcc gccggcggcc ccggcagcag gatgaacatg ggaagggaac
     3961 cgctggcagg catcaccagt ccgggcgccg tcccgttgga attctccagc accgcggcgc
     4021 catccggaac catggcctgc ttgaaattgc actccgcaat gggaatcccc ctccgtttaa
     4081 aataactctt cagccactcc gccacatgct cgtcacggtg catgtccacg ccgcaaacgt
     4141 cgcacagggc ttcccgggtc acatcatcgc tcgtcggccc cagtcccccg cttacaatca
     4201 cgatatctgc gcgccgggcg ctctccctca tggcctcgtt aatagcgtac ccgtcaggca
     4261 ctacggttac cctccccacg cggaaccctg cctcaaataa tctgttgccc agccatgcgg
     4321 catttgtatt cacaatactc cccagcaaaa tttccgtccc ggtgttgatc aattcaaccc
     4381 tgatctgttt catgcagcca gtatgctccc tcaagcgctc cgtgacatgc aacaatcctg
     4441 ccatgtatca taaaaaactt catttaaacg ccccgtaaga tacattaggg gcactgacaa
     4501 agaccatgaa cacaccttcc cggccccggg ccaccaaaat catctgtact attggaccag
     4561 caacagacac ttccgacatg ctgggccagc ttattgaagc cggagccaac gtcttccggc
     4621 tcaacatgag ccattccaaa cacgactggg tccgggaagt cgtcttccgc atccgccgga
     4681 aagcagcgga actgaaagcg aacgtagcta tcctctttga cctccagggg ccttccattc
     4741 gcacgggaga tctgatggaa ccctaccatt tgaaaaaggg agacaccgtg gaaatccgcc
     4801 tgggcacagc caagccggaa cttccttttt ccaccacggt aaactatgac ggcctcctgc
     4861 aggatgttca ggcgggccat accatggtgg tggataacgg aggcatcctc atgcgcattg
     4921 cggaagtgcg cccggacagg ctcatctgcg acgtgctcac ggaaggcgta ttcggctccc
     4981 gccgccacat caaccttccc ggcgtagcgc tgcggctccc ggccctgacg gaaaaagact
     5041 tggcggacct ggccgtggcc gtagaatgcg agacggacta cgtagccatg tccttcgtcc
     5101 gggatgccgc ccatattgcc cagctccggg aacacatcga caacttggga ggcaaggccc
     5161 agatcgtcgc aaaaattgaa gaccagcagg ctgtccgcca cattgacgat atcatcctgg
     5221 ccgcagacgt catcatggtc gcgcgcggag acctgggcat tgaagtcagc attgaagaac
     5281 tgcccatcat ccagcgccgc atcgtccgcc attgccacag gctgggacgc cgctgcatcg
     5341 tggcaacgca tatgctggaa tccatgataa cccagcccac ccctacccgt gcggaagtca
     5401 cggacgtttc caacgccatc tttgaacaag tggatgccgt catgctttcc ggagaaacct
     5461 ccgtaggcca ttaccccgta cgctgtgtgg aagtgctgga ttccatcgcc caccgcatgg
     5521 agtgctccgg caatctcaac ttcgcggcat ccgccatcct gaacggagac cgccagaaag
     5581 ccaccaaggc agccatctcc ctggcggact ccgtggaaaa cgcctgtctg atcgtcttta
     5641 cccgccgcgg cctggccgcc acgcaggcag ccgtcctcag gccggaacgg gctgccattt
     5701 tcgccttcag caatgaccct gtcgttgtca ggcaactcgc cctggcgcgt gacgtaaccg
     5761 cctttgaatc acccttcctg aaagatccgg cccgcatgat ttccaccgcc ctggacctgc
     5821 tgaaagaaaa agggctgatc tcctccggcc agcctgtcgt catccagggg gacagcctgc
     5881 aaggagaact gctggcggac tccatcatct tcatgcgcgt ggaataatgg cctgcacctt
     5941 tcctatagag cgggagacgg ccgtgtggcc cccgcccgcc cccctcaggg aacttctgct
     6001 ggatgcggat ccggacgaac gccggatagc ggatcatctg gcccacggcg aactttacgt
     6061 cgcaaaaaac tgcgggactg ccgccggagt cattgttatc cgtcagacga gggaacatac
     6121 atgggaaatc atgaattgct ccgtagctcc tgaatataga aggcagcatt gcggaacagc
     6181 cctggtgcgg cacgccctgg acatcatcag aaacaaaggg ggccggtatg cggaagtggg
     6241 aacgtccgat gcgtctccgg ggccaatggc tctctatgaa agctgcggat tccagatttc
     6301 tggcgttatc aacaaccatt tcacggacaa ctatccggaa cccgtctggg acaatggcgt
     6361 gcaatgtatt gacatgatcc gcatgcgtgc ggacctctcc cttcggaaag aaggagcgca
     6421 tcatgctgct caaaaagcat aaaccacgct gactcccgtc acccagctat tgccctttac
     6481 cagcgttctt ccatacacgc ggttcacccc gtgggaaccc ttgccgagcc agtgcaggcc
     6541 aacgaaaggc tctataaaaa cattcccgaa cagacggtaa ttcgccgtca ccctgaaata
     6601 gagagactgc cagccattgc tgctggaagg ccaataggaa gaactcaaca cggcgccgaa
     6661 tttaaaaccc atataaaggt tttccgtcat ccggaaatcc gatcccgtac acatgtcaaa
     6721 ccaccagccc gtgattccgt aaaaggaata tgcggcactt ccctgcgcat aaaaaccggg
     6781 aaggaatccc acatcatggc gcataatcag tgccgcttca tacgttgtcc ccccattgcc
     6841 gaacaaacgt tcggaagcaa accccgccaa tccgccgtca ttcgcacgaa aggataatgc
     6901 cgcatacgta gcttttccga acttccgctg aagaccaaga tccaccatcg tctcattctc
     6961 tatatcaact gccggatgcc ccagaaagat ctcctccttc agagccagaa tcagggaata
     7021 ccggtcattc aaatcgcttc tccaatccag ccgcaaagga gccgtaggat tacagcccgc
     7081 gggaatcagc cccctgaact cgtaattagt ggaatacccg gctttgatac tgccgctgac
     7141 cggtcttaaa gcaggagcgg aaggaacctc cggaatgcgt gattccgccg aagaaacggc
     7201 catggaagcg acagagacga tgcccgctcc aaagagaaaa gaaaaaaaga aattcataac
     7261 atcgaaatgc cgtgcggaaa gtattctgga taaaaaaact cctcttggca acaagcaaaa
     7321 acgccgctgt tttctgctct gcttcaggaa acatcattca ggcctgcctg ctgccaactt
     7381 cagaaagaat aaacgagacc agccccggct actactccga aattcttata aggcttgtcg
     7441 cccctgtccg aacctcggtt ggccttcaag gcgccctctc cggcccagtt gaagctgacg
     7501 aagggaacga gagacaggtt cttggcaccc agtttcaccg gaagttcagc cttcagccac
     7561 caggcctggg aaccgttgga cataaaaagg gatttattgt caaaataaga ggaagtggca
     7621 gacatcccgg cgctcaatac aaaatcgatg tcggagcaga tagctgcctg ataccccaca
     7681 tgcggccgga accaccagcc ggtcatgccc tggaaggcat agctcgtggt tacgccggca
     7741 aaccagtttc cgggcaaacc gtaatccagc gtcagataaa attcctgaat gacggaatgg
     7801 gtattcctta tatacacatc ttcatcattc atccaggtgg cgttataatc atatctggca
     7861 aaattcccaa gcaggccgcc gtgaatcagg ttccagccaa gcgtcgtgga cagattcttc
     7921 agtaaaccat cccggttctt ccatccaaaa agaaaatcgg tctcgttatg aaaagagaaa
     7981 tctctttccc ctgccatgtg gtgtcctgaa atcaggaacg tacaagatgc cgccccaaca
     8041 acggcattac aatcatccag tttataattc aaatccacac tggcaggaat aattccatct
     8101 ccttccgtca gtgaatgaga agccacaatg ccgcggcagg tgtaattgga agcatacccg
     8161 gcatcaactt ccccggacaa aggacagtcc tgttccacgg gaacagaaac cggagaaaca
     8221 gttttaacgg aaattcctgc aaaagcggaa gcggaaagaa aaacggcacc cccccacaat
     8281 accaatgata tttctgaatt gcatcatcac gcttcaaatg aaaatttgga tatcggcgca
     8341 ttgcaaaaaa cggagttcat aaaaaacgat acgccgccag caattaaaaa aagcgcggcg
     8401 gatttctccg ccgcacctga aaatataatt caatcgcagg ccggatggct tagaaagaat
     8461 agacaaggct ggctccagcc actacgccga agttcttgta aggcttgtct cccttatcca
     8521 ggcccttatt gaccttcaat gcgccacagc cggcccagtt gaagctgacg aagggaacaa
     8581 gggacaggtt cttcactccc agcttcaccg gaagttcagc tttcacccac caggcctgag
     8641 cgccattggc ctgctcgaac gcagcgccaa aatagctgga cgtggcagac atcccggccg
     8701 tcacgacaat gtccgtgacg gggcagaggg cggccttgta gccaacataa ggttggaacc
     8761 accagccggt catgccctgg aaggcatagc tcgtggttac gccggcaaac cagttctgag
     8821 tcagatcgta attcagattc aggtaaaatt cctgcgtgac ggaatgagca tggtcatact
     8881 tggcaaagct gcccagcaaa ccgccgtgaa tcaggttcca gccaagcgtc gtggacaggt
     8941 tcttcagcaa accgtcctta ttctgccagc caaggttgaa attggtctca ttgtggaagg
     9001 aagtatccct gtcccccatg agatggtggc ctgaagtcaa ggtcgtataa gaagcggaag
     9061 ccacgatgga gttggcatcg cacagcttgt aattcagatt gacaccggcg gggatgacgc
     9121 tatccccttc cgccaacgcg tgggaagcca caatgccgcg gcaggtgtaa ttggaagcgt
     9181 agccggcgta aatatcgccg gacagcgggc tggaagtcgc cacaggagcg ggaaccggag
     9241 tcggagcaac aatcttgacg ggagtacccg caagagcagc tccggcaaag gatgccacaa
     9301 ctgccgcaac actgatgatg ttcttggttt gcatgactat tacttggtta gggtattaat
     9361 tgttgatttg ctaacatgga aagaaaaatg ctttttcccc ccttgttaag aaaagtgaaa
     9421 cattttcgtc acaaaagtaa agaattttct cttgataaat cagcaagtca ttgatttcac
     9481 attatttaca tccaccagcc ccatgacaaa aaatccaatt ctccatatct tgccaaacct
     9541 tcccttccaa ggcctggccg gatacagaca ctccgcacca tccagaagcc tgtcttcttt
     9601 cagggcagga gaaggattca ccaaaaccct ccgcctgcag gaaagcagca tgggcaaatc
     9661 cgcggcactg tcgctgtagg caacggcgtt ttccagaaca ccatgttccg gcagtacatt
     9721 ccgctcccgc agacgttcca ccttcactgc ccccttatta ttaccgttcg gcagttccgg
     9781 cattgccggc atgcgttcct ccagaagcac atccgttccc agaacttcgt caaatcccag
     9841 cagttcgccc aggggcttca cgtaaaatga tggagaagcg gaaaccatga gacacaaata
     9901 ccccttcttc cgatactctg ccagccgctc cttcagctcc ggataaatcc attcctgcgc
     9961 catctcggca aacttccgtc catattcccg caccgtttca gcgggaagcc cccacaaata
    10021 catgagataa gccctcttca tccggttctc atcccatatt ctcaaaatat agagcggaat
    10081 acaggc
//
LOCUS       DAOMSU010000056        10040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_416_length_10040_cov_3.060791, whole genome shotgun sequence.
ACCESSION   DAOMSU010000056 DAOMSU010000000
VERSION     DAOMSU010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10040
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_416_length_10040_cov_3.060791"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            325..2292
                     /locus_tag="PK077_03890"
     CDS             325..2292
                     /locus_tag="PK077_03890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN23470.1"
                     /translation="MRIDVVTDTYEPDVNGVALTLGRLVGGLRMRGHLVHVMRASERN
                     GSFNPGETAMPSLPLPMYHEVKIGLPSADRFRARWMKKRPDVVYVATESPMGASAVKA
                     ARTLEVPVVMGFHTNFHQYMKDYHFSRLETAAVNYLRKLHNRAGMTVVPTEEMRRTLE
                     GLGFERLSVMGRGVDAVLFDPARRDASLRQSWGVWRDEVVFGVVGRLAREKNLVAALG
                     LYTRLQREFPDCGMKMVVVGDGPMMNSLRSEFPDAVFCGMRRGEDLARHYAAMDVLLF
                     ASETETFGNVLLEGMASGLATVSYRYAASADVVLDGINGLQAEKGDEEGFYSAMRRLL
                     EDGEMIRRLGKQARRTVNSRTWDSIHDRFEELLASVAREENGTGCARPPRDAVLECRT
                     VFLSDLHLGTKDCKADECRKFLKHVRAGKIVLVGDIVDAWALSRGSRWRRRHTRFVRT
                     LLKKMEQEDVEVLYLRGNHDDILEKFLPFHLGGLKIAREYVHQAVDGRRYLCVHGDGF
                     DAISTNHRWLAMLGSLGYDVLLMVNRFYNKYRAWRGREYYSVSRAIKGRVKSAVNFIG
                     KYEEQLQRLAVKRQCDGIIAGHVHHPADTMVGKVRYLNCGDWVETMSAVLEYGDGRME
                     TVLYKDFMKRLAYGKCGTMEDAETSPGTGDS"
     gene            2597..4525
                     /gene="dnaK"
                     /locus_tag="PK077_03895"
     CDS             2597..4525
                     /gene="dnaK"
                     /locus_tag="PK077_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRN23471.1"
                     /translation="MAKILGIDLGTTNSCMAVMEGGQGTVLENSEGARTTPSIVAFTK
                     SGERLVGQAAKRQAVTNPKNTVFSSKRLIGRKYSELTEEDKKVPYEIVEAPNGDAYIR
                     VDVGGEKKTFSPQEIASMVLAKLKADAESKLGETITEAVITVPAYFNDAQRNATKAAG
                     EIAGLKVRRIINEPTAAALAYGLDKKSNENIAVYDLGGGTFDISVLEIGDGVFEVKAS
                     DGDTHLGGDDWDNAIINWIIGEFKKDSGMDLSNQPDAIQRIKEEAEKAKIALSSTQSY
                     DISLPFITADASGPKHIQLTLSRPKLEQLTEDLLDRTRKPVLDCIAASGLKTGDIDEL
                     VLVGGMTRMPAVQEMAHTLAGKEPHKGVNPDEVVALGAAIQGGVLQGDVNDVLLLDVT
                     PLTLSIETMGGIATPMIERNTTIPVRKSQVFSTAADNQPAVDIRICQGERKMFEDNKL
                     LGNFKLDGISPAPRGVPQIEVTFDIDANGILHVSAKDKGTGKEQKISIQGSSGLSKDE
                     IERAKRDAEAHAEEDKKRAEEIDIINQADSLCFSVERQLKDMGDKLPADLKREIEDKV
                     TRLKEAVSKKDVKAIKAGKEDLESRLEALYKAAEAAQQSAGAAGPMPGAPAEEEPSDG
                     PRKAKGRVVDAEIVDDDK"
     gene            4621..4911
                     /gene="groES"
                     /locus_tag="PK077_03900"
     CDS             4621..4911
                     /gene="groES"
                     /locus_tag="PK077_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRN23472.1"
                     /translation="MANIKPLGQRVLVKRIEAETKTAGGLFLPDTAKEKPQEAEVISV
                     GTGGRDEKGALIEFTVKPGDRVLISKYGGTEIKLDGEDYLILSENDILAIIG"
     gene            4954..6609
                     /gene="groL"
                     /locus_tag="PK077_03905"
     CDS             4954..6609
                     /gene="groL"
                     /locus_tag="PK077_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420446.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRN23473.1"
                     /translation="MMAKQIQFDETARQALLRGVEQIAKAVKSTLGPAGRNVVIDKKF
                     GSPLITKDGVTVAKEIELEDPFENMGAQLVREVSSKTNDVAGDGTTTATVLAESIYRE
                     GLRNVTAGANPISLQRGIMKAADSVVEELKKISKPVDSSKEVAQVATVSANWDAEIGN
                     IIAEAMDKVGKDGTITVEEAKGIETTLDVVEGMQFDKGYLSPYFVTNAETMEAVLENP
                     YILIHEKKINNLKDFLPLLEKVAKSGRPFLVIAEDIEGEALATLVVNRLRGVLNICAV
                     KAPGFGDRRKAMMEDIAILTGGKCITEDLGIKLENVGIEDLGQAKRVVVSKDETVIVE
                     GSGKSSDIEARISQIRRQIKDTTSDYDREKLQERLAKLAGGVAVIHVGAATETEMKEK
                     KARVDDALHATRAAVEEGIVPGGGVALIRAQKAIDTLKLEGDEATGAQIVYRAVEAPL
                     RQLACNAGREGALIVANVKGMKNTAEGYNVATDKYEDLLSAGVVDPTKVTRSALQNAA
                     SIAGLLLTTECVIADKPEKKSCSCGSGASDMGGMGGMGGMGMM"
     gene            6784..7236
                     /locus_tag="PK077_03910"
     CDS             6784..7236
                     /locus_tag="PK077_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HRN23474.1"
                     /translation="MKKLHKNILFAAAVLVMPSALADIPESSVPAAAVSSIKGAYPNA
                     GPIEWDSEMHGMERIYEAEFRINGFQFDVKIAPDGKIVRIKEEIAASSLPEAVRASVL
                     KRYPGCRIKEAEKITEGELVRYKVEVADSRDDYDVLIAPDGNILHVDR"
     gene            7408..9639
                     /locus_tag="PK077_03915"
     CDS             7408..9639
                     /locus_tag="PK077_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRN23475.1"
                     /translation="MDPIYYFIIGLILVLSCFDLVVGVSNDAANFLNSAVGSKAAPRR
                     TIVLVAALGIIIGSLFSSGMMEIARSGVFMPARFSFHDIMLIFLAVMLTDVILLDVFN
                     TFGLPTSTTVSIVFELLGGAVAAALFKIWSGEPGVAQELSSYINSSKALAIISGIFSS
                     VFIAFICGITVMWISRLIFSFNYQKSFKYLGAVWCGVALTAITYFAIFKGLKGSTLVT
                     KDMIHHLDAHIWLYVCCSLAFWTVLMAVLQNLCKVNILKVSVLAGTMALALSFAGNDL
                     VNFIGVFMAGQSSMEIAAAAAAQGADLTTLSMGGLMAPVTADWRYLLGAGVIMVLALM
                     FSKKAQTVTDTEVNLARQGGGVERFGSVPPARMAVRYALNASRAVEKIMPSCVGRFIE
                     KRFRPVPEGPDNGASFDLIRASVNLTVAALLISLATSLRLPLSTTYVTFMVAMGSSLA
                     DKAWGRDSAVYRITGVITVISGWFFTAFAAFSMCFIVAACILYGGLFGIIAMCSLAAF
                     LLLKSSRLHRKRTQAAAQAQAANYRDPAAAARYNEEIIELMKRMAEIYEMNLEALNVE
                     DRKRLKKLRKEARGIRRSLSDKMAMEVMPVVRELPDKEADRGKRYVQMVEYATSVFES
                     LSNITTASHAYIDNNHEGLDLERIELLRGMNSRVSSLYPRFREMMESNDYTGLDECLA
                     GMDALDEEFAEAVKQQIILRPEDASDMRRALLYLNLLNETRAMIRKVLLLAKIQRKFV
                     LGW"
     gene            complement(9895..9968)
                     /locus_tag="PK077_03920"
     tRNA            complement(9895..9968)
                     /locus_tag="PK077_03920"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9934..9936),aa:Gly,seq:ccc)
BASE COUNT         2317 a         2664 c         2908 g         2151 t
ORIGIN      
        1 ttagccccgg atacccgaag ctggacatgg ctgctgcgcg aaccggacag cagcgtagag
       61 gagtaccgcg catggattga gagcgtttga tattcaatgg actgtttctt tttttgcagt
      121 aatacgcagt tttggaagat gtcctgataa aaaaagaggg ctcatgatac gtacccgtac
      181 gggaaaaacg ggaaggccgt ggcggaattg ctccggagcg gctgttgatt ctgccggggc
      241 tgcgagcata accggatgtt tgtgacggtt tcgtttccgg ctgcatcttg tttgaggaag
      301 ccattccgct catggtatcg gtacatgaga attgatgtgg taacggacac gtatgaacct
      361 gatgtaaatg gagtggccct gacgctgggg cgtctggtcg gcggcctccg gatgcgcggg
      421 catttggtgc atgtgatgag ggcttctgaa cggaacggct ctttcaaccc tggggagacg
      481 gctatgccgt cccttcccct ccccatgtac catgaggtga agatagggct gccttctgca
      541 gacaggttcc gcgccagatg gatgaaaaaa aggccggatg tggtatacgt ggctacggaa
      601 agccccatgg gagcgtctgc cgtgaaggcc gcccgtacgc tggaggttcc cgtggtgatg
      661 ggattccata ccaatttcca ccagtatatg aaggattacc atttctccag actggaaacg
      721 gccgccgtga attacctgcg caaactccac aacagggcag ggatgacagt ggttcccacg
      781 gaggaaatgc gccgtacgct ggaagggctg gggtttgaac gcctttccgt catggggcgc
      841 ggagtggatg ccgtcctgtt tgatcccgcc aggagggatg cttccctgcg ccagtcatgg
      901 ggcgtctggc gggatgaagt ggtatttggc gtcgtggggc gtctggcgcg ggaaaagaac
      961 ctggtggcgg ccctggggct gtacacgcgc ttgcagaggg agtttccgga ttgcggcatg
     1021 aaaatggtgg tggtagggga cggccccatg atgaacagcc tgcgcagtga gtttcctgat
     1081 gccgtttttt gcggaatgcg ccgtggggaa gacctggcgc gccattatgc cgccatggac
     1141 gttttgctgt tcgccagtga gacggaaact ttcgggaacg ttttgctgga aggcatggcc
     1201 agtggtttgg ctacggtcag ctaccggtat gccgcttccg cggatgtggt gctggacggc
     1261 atcaacggcc ttcaggcgga gaaaggggat gaagaggggt tttattccgc catgcgccgt
     1321 ctgctggaag acggggagat gatccggagg cttgggaaac aggcccgcag gacggtgaat
     1381 tccagaactt gggattccat ccatgacagg tttgaagaac tgctggcttc cgtggcccgg
     1441 gaggaaaacg ggaccggctg cgcccgcccc ccgcgggatg ccgtgctgga atgccgcacg
     1501 gtttttttgt cggaccttca tctgggaacg aaagactgca aggcggacga atgccggaag
     1561 tttctgaaac atgtccgggc tggaaaaatt gtgctggtgg gggatattgt ggatgcctgg
     1621 gctttgtccc gcggcagccg ctggcggcgg aggcatacgc gctttgtccg caccttgttg
     1681 aaaaagatgg aacaggagga tgtggaagtc ctgtacctgc gcggaaacca cgatgatatt
     1741 ctggaaaaat tcctgccttt tcatctgggc ggtttgaaaa ttgccaggga gtacgtgcac
     1801 caggctgtgg atggaagacg ctacctgtgc gtgcatggag acggttttga cgccatttcc
     1861 accaatcacc ggtggctggc gatgctcggc tctctgggtt atgacgtttt gctgatggtc
     1921 aacaggtttt acaacaaata ccgcgcctgg cggggcaggg aatattattc cgtttcccgc
     1981 gccatcaagg gccgcgtgaa atccgccgtc aattttatcg gaaaatatga ggaacagctt
     2041 caacgcctgg cggtaaagag gcagtgtgac ggcatcatcg ccgggcatgt gcatcatccg
     2101 gcggatacca tggtgggaaa ggtgagatat ctgaattgcg gggactgggt ggaaaccatg
     2161 agcgcagtgc tggaatacgg cgacggccgt atggaaacgg tgctttacaa ggattttatg
     2221 aagcgcctgg catatggcaa atgcggaacc atggaggacg cggaaacttc tcccggcacg
     2281 ggggattcat gacggaacgg cctttatccg gtttcccccg gaagtgggac aaaatgactt
     2341 tgtgtgacat tttgtctctt ttctggtgtg acattatgtc ccgttttcct ttgtcgggaa
     2401 aatagaggaa tgaaaggaaa aacagcgtaa tatgattata atatgacatt tgcgtttatt
     2461 tttcagtatg ccttgccaga ttggcatgcc atttgcaaca aaagaggcaa gaatatgagt
     2521 ttgcctgcat gatgcttcag gtcatgagga aatcaaatct caacgaaatc cattcaaaac
     2581 aatctaaaat aacgatatgg ctaaaatatt aggaatcgac cttggcacga ccaactcgtg
     2641 catggctgtg atggaaggcg gtcagggtac cgtacttgaa aacagcgaag gtgcgcgcac
     2701 cactccctcc attgttgctt tcaccaaaag cggtgaacgc ctcgtggggc aggccgccaa
     2761 acgtcaggcg gtaaccaacc cgaaaaacac cgtgttttcc tccaagcgcc tcatcggccg
     2821 taagtacagt gaactgacgg aagaagataa aaaggtgccg tacgaaattg tggaagctcc
     2881 caacggagat gcctacatcc gcgtggatgt gggcggtgaa aagaagacct tttcccccca
     2941 ggaaatcgct tccatggtgc tggccaagct gaaagccgat gcggaatcca aacttggcga
     3001 aacgattacg gaggccgtga ttaccgtccc cgcctatttc aatgacgccc agcgcaacgc
     3061 gaccaaggcc gccggtgaaa tcgcaggcct taaagtgcgc cgcattatca atgaaccgac
     3121 ggcggcggcc ctggcctacg gcttggacaa aaagtccaat gaaaacattg ccgtatatga
     3181 cctcggcggc ggcacctttg atatttccgt gctggaaatc ggcgacggag tgtttgaggt
     3241 gaaggcttcc gacggtgaca cccacttagg cggcgatgac tgggacaacg caatcatcaa
     3301 ctggattata ggcgagttca agaaagactc cggcatggat ctttccaacc agcctgatgc
     3361 cattcagcgc atcaaggaag aagcggaaaa ggccaagatc gccctttcct ccacccagag
     3421 ctatgacatc agcctgccgt tcatcacggc ggacgcttcc ggtcccaagc atatccaact
     3481 gacgctgagc cgccccaagc tggaacagct cacggaagac cttctggacc gcacccgcaa
     3541 gcctgtgctg gactgcattg ctgcgtccgg gttgaaaacc ggggacattg acgagctggt
     3601 gctggtgggt ggcatgaccc gcatgcccgc cgtgcaggaa atggcccata cgctggccgg
     3661 caaggaaccc cacaagggcg tgaacccgga tgaagtggtg gccctgggcg ccgctattca
     3721 gggcggcgtg cttcaggggg acgtgaatga cgtgcttctg ctggacgtga ctcctctgac
     3781 cttgtccatt gaaacgatgg gcggcatcgc cactccgatg atcgaacgca acacgaccat
     3841 tccggtgcgc aagagccagg tgttctccac cgccgctgat aaccagcccg ccgtggatat
     3901 ccgcatttgc cagggcgaac gcaagatgtt tgaggataac aagctcctcg gcaacttcaa
     3961 gctggacggc atttcccccg ctccgcgcgg cgtgccgcag attgaagtga cctttgacat
     4021 tgacgccaac ggcattctgc atgtatccgc taaggacaag ggcaccggca aggagcagaa
     4081 aatttccatc cagggctcca gcggcctttc caaggatgaa attgagcgtg ccaagcgcga
     4141 tgcggaagcc cacgcggaag aagacaagaa acgcgctgag gaaattgaca tcattaacca
     4201 ggctgactcc ctctgcttct ccgtggagcg ccagcttaag gatatgggtg acaagctgcc
     4261 cgcagacctc aagcgtgaaa tagaagacaa ggtaacgcgt ctcaaggaag ccgtctccaa
     4321 gaaggacgtc aaggcaatca aggctggcaa ggaagacctg gaatcccgtc tggaagccct
     4381 gtacaaggcg gcggaagccg cccagcagtc cgccggggca gccggcccca tgccgggcgc
     4441 ccctgccgaa gaagagcctt ccgacggtcc ccgcaaggcg aaaggccgcg tggtggacgc
     4501 tgaaatcgtg gacgacgata aataagcaac ccccttcccg ccggaggggg aagcaattcc
     4561 ccctccggcg gaacattaca tcccgtatta ctcatctaca acaacatcaa aaaacatact
     4621 atggcaaaca tcaaacccct aggacaacgt gtgctggtca agcgcattga agctgaaacc
     4681 aagacggccg gcggcctgtt cctgccggac accgccaagg aaaaacccca ggaagccgaa
     4741 gtgatctccg taggcaccgg cggccgcgat gaaaaaggcg ccctgattga atttaccgtg
     4801 aagcccggag accgcgtgct tatttccaag tacggcggca cggaaatcaa actggacggg
     4861 gaagactacc ttatcctgtc tgaaaacgat attctggcca tcatcggcta aatggcacca
     4921 cacacacatt caacatcatt ttaaatattt aacattatgg ctaaacaaat ccaatttgac
     4981 gaaaccgccc gccaggctct gctccgcggc gtggaacaga ttgccaaggc tgtcaagagc
     5041 acgctgggcc ctgccggccg caacgtagtg attgacaaaa aattcggttc ccccctcatc
     5101 accaaggacg gcgtaaccgt ggccaaggaa attgaactgg aagacccgtt tgaaaacatg
     5161 ggcgcccagc ttgtccggga agtatcttcc aaaaccaatg acgtggccgg cgacggcacc
     5221 actaccgcta ccgtgctggc tgaaagcatt taccgcgaag gcctgcgcaa cgttactgcc
     5281 ggggctaacc ccatctccct ccagaggggc atcatgaagg ctgcggattc cgttgtggaa
     5341 gaactcaaga agatcagcaa gcctgtcgac tccagcaagg aagtggccca ggtcgctacc
     5401 gtctccgcca actgggacgc cgaaatcggc aacatcatcg cggaagccat ggacaaggtg
     5461 ggcaaggacg gcaccatcac cgtggaagaa gccaagggca ttgaaactac gctggacgtg
     5521 gtggaaggca tgcagtttga caagggatat ctgtctccct acttcgtgac gaacgcggaa
     5581 acgatggaag cggtgctgga aaacccctac atcctcatcc acgaaaagaa aatcaacaac
     5641 ctgaaggact tccttccgct gcttgaaaaa gtggccaaga gcggccggcc cttcctggta
     5701 attgcggaag acattgaagg cgaagccctc gccaccctgg tagtcaaccg tctgcgcggc
     5761 gtgctgaaca tctgcgcggt caaggctccc ggcttcggcg accgccgcaa ggccatgatg
     5821 gaagacatcg ccatccttac cggcggcaag tgcatcacgg aagacctggg catcaagctg
     5881 gaaaacgtgg gcatcgaaga cctcggccag gccaagcgcg tggttgtttc caaggatgaa
     5941 accgttatcg tggaaggttc cggcaaatct tccgatattg aagcccgcat ttcccagatt
     6001 cgccgccaga tcaaggacac cacgtccgac tacgaccgcg aaaaactcca ggaacgcctg
     6061 gccaagctgg ccggcggcgt ggccgtcatc cacgtgggtg ccgctacgga aacggaaatg
     6121 aaggaaaaga aggcccgtgt ggacgacgcc ctgcacgcta cccgcgctgc ggtggaagaa
     6181 ggcatcgttc ccggcggtgg cgtggcgctg attcgcgccc agaaagccat tgacaccctc
     6241 aaactggaag gtgatgaagc aaccggcgcc cagatcgttt atcgcgctgt ggaagccccg
     6301 ctccgccagc tggcctgcaa tgccggccgc gaaggagccc tcatcgtcgc caacgtgaaa
     6361 ggcatgaaga atactgccga aggttacaac gtggccacgg acaagtatga agacctgctt
     6421 tccgccggcg tggtggatcc gaccaaggtg acccgttccg ctctgcagaa tgcggcctcc
     6481 atcgccggcc tgctgcttac cacggaatgc gtcattgctg acaagcccga gaagaagagc
     6541 tgcagctgcg gctccggagc ttccgacatg ggcggcatgg gaggaatggg cggcatgggc
     6601 atgatgtaag ccccgtccgt ttcttcgaga ccaaggcaat cggcttcaag gctccccggt
     6661 ttttccgggg agcctttttt aatatgcttc atcaggagcc ggtttcggga aaaaagctgc
     6721 acaggatgtt cagagtttag aaaaatattg aatgattata gaacatattt gtctaatcat
     6781 aacatgaaaa agctgcataa gaacatattg tttgcggcgg cggtactggt catgccttct
     6841 gccctggccg atatcccgga gtcttccgtt ccggctgccg ctgtttccag tatcaagggt
     6901 gcttatccga acgccggtcc cattgaatgg gactctgaaa tgcatggcat ggaaaggatc
     6961 tacgaagccg aatttagaat taacggattc cagtttgacg tgaagattgc tcctgatggg
     7021 aaaattgtga ggattaagga agaaatagct gcttcctctc ttccggaagc cgtccgtgcg
     7081 tccgttttga agcgttatcc gggatgccgg attaaggaag cggaaaaaat tactgaagga
     7141 gaacttgtcc gttacaaggt ggaagttgcg gacagccgtg acgattatga tgtgctgatt
     7201 gcccctgacg gaaatatcct tcatgtggac cgttaggctt tttgtttttt acatttaatg
     7261 aaagcgggag gagggcggat gaatccgctc tcctcctttt ttgaggaccg gaagcagggg
     7321 cgtatgggaa aatgtgacgg aatgttcacg ttttaaaacg ggcgtgcggg agcggcctcc
     7381 tgtaatagag ggccgtttac tacgcatatg gacccaatct attactttat cataggactg
     7441 atactggtgt tatcctgctt cgacctcgtt gtcggagttt ccaatgacgc cgccaacttt
     7501 ttgaactccg ccgtcggttc caaggccgct ccgcgcagga cgattgtgct ggtggccgct
     7561 ttgggtatca tcatcggctc gctgttctcc agcggcatga tggaaatcgc cagaagcggc
     7621 gtattcatgc cggcccggtt ttccttccat gacatcatgt tgatctttct ggcggtcatg
     7681 ttgacggatg ttatactgct ggacgttttc aacacgttcg gccttcccac ttccaccacg
     7741 gtttccattg tttttgaact gctgggcgga gccgtggccg cagctctctt taaaatttgg
     7801 tccggggaac cgggagtcgc tcaggaattg tccagctaca tcaattcctc caaagctctg
     7861 gcgatcatct ccggcatttt ttcctccgtt tttatcgctt tcatttgcgg gataacggtt
     7921 atgtggattt cccgcctcat cttctccttc aactatcaaa aatccttcaa atacctgggc
     7981 gccgtctggt gcggcgtggc cctgacggct atcacctact tcgccatttt caaggggctg
     8041 aagggaagca cgctggtcac caaggatatg atccaccacc tggacgccca tatctggctg
     8101 tacgtttgct gcagcctggc attctggacc gtcctgatgg ccgtgctcca gaatctttgc
     8161 aaagtcaata tcctgaaagt atccgttctc gcgggcacca tggccctggc cctttccttt
     8221 gccgggaatg acctggtcaa cttcatcggc gttttcatgg cggggcagtc ttctatggaa
     8281 attgcggccg ccgcagcagc gcagggggcg gaccttacca ctctgtccat gggcggtctg
     8341 atggcccccg tcacggcgga ttggcgttac ctgctggggg cgggcgtcat catggtgctg
     8401 gccctcatgt tctccaaaaa ggcgcaaacc gttacggata cggaagtcaa tctggcgagg
     8461 cagggcgggg gagtagagcg gttcggctcc gtccccccgg cccgcatggc ggtgcgctat
     8521 gccctgaatg cttcccgcgc ggtggaaaaa atcatgccct cctgcgtcgg gaggtttatt
     8581 gaaaaacgct tccggcctgt gccggaaggg ccggataacg gtgcatcttt cgacctcatt
     8641 cgcgcttccg tgaatttgac ggtggctgcc ctgctgattt ctctggccac gtcccttcgg
     8701 cttccccttt ccaccacgta tgtgacgttc atggtggcga tgggttcctc cctggcggac
     8761 aaggcctggg ggcgggacag cgccgtgtat cgcatcaccg gagtgattac ggtgatttca
     8821 gggtggttct ttacggcctt tgccgcgttt tccatgtgct tcattgtggc ggcctgcatc
     8881 ctttatggcg gtctcttcgg catcatcgcc atgtgttcgc tggcggcttt tctgcttctg
     8941 aaaagttcca ggctccatcg taaaagaacc caggctgcag ctcaggcgca ggccgccaat
     9001 taccgggacc cggctgccgc cgcacgctac aatgaggaaa ttattgaact gatgaagcgc
     9061 atggcggaaa tctatgaaat gaatctggag gccctgaacg tggaggaccg gaagcgtctg
     9121 aaaaaactcc ggaaagaagc gcgcggcatc cgccgttccc tgagcgataa aatggcgatg
     9181 gaagtcatgc ccgtcgtccg tgaactcccg gacaaagagg ccgaccgagg caagcgctat
     9241 gtgcagatgg tggaatacgc tacctccgtc tttgaatccc tctccaacat tactacggcc
     9301 agccatgcct atatagacaa caaccatgaa gggctggatt tggaaaggat agagcttctg
     9361 cggggaatga acagccgtgt ttcctccctc tacccccgtt tccgggaaat gatggaaagc
     9421 aatgactata ccgggctgga cgaatgcctg gcaggcatgg acgccctgga tgaggaattt
     9481 gcggaagccg tcaaacagca gattattctg cgtccggaag acgcctccga tatgcgacgg
     9541 gcgcttctct atctcaacct ccttaatgaa acccgggcga tgatccgcaa agtgcttctt
     9601 cttgctaaaa tccagaggaa atttgttctt ggctggtagc ctctccctgt aattcacgtc
     9661 cgtgccggaa gcattcgccg ccggcacgga cgtattgccc ggactgttcc cgtattggga
     9721 gaggctgcca ggtctttttt attcacacgg aacaaggctt ctttgcggat aaaccatgtc
     9781 tgctgcctgt gggacaatgt cttgaaaggc cgtttccagg cgttgccatg tatgttgccg
     9841 tctcaagaaa acaaaaaacg caagctttga taaacaataa cttgcggaac aaactggtac
     9901 gggcgatggg aatcgaaccc acgtctcaag cttgggaagc tcgcgtccta ccattgaacg
     9961 acgcccgcat gaaatgcgga tgcctattta gcggcagaag gcccggatgt caagaccaca
    10021 gaaaaaacgt atcctctttt
//
LOCUS       DAOMSU010000057        10032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_417_length_10032_cov_2.860980, whole genome shotgun sequence.
ACCESSION   DAOMSU010000057 DAOMSU010000000
VERSION     DAOMSU010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_417_length_10032_cov_2.860980"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PK077_03925"
     CDS             <1..667
                     /locus_tag="PK077_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23476.1"
                     /translation="KQNTGNNVAPGFKKALQIQCKWMLLCLRKADAEKNERELNVSSS
                     VLSMLDEVAANAKDVGEQLPMVSGASEVIRTYLNIGDYRSETLPDNLMDLNTIFDRVL
                     LVPYKEKKDVENFRKLWNKRIALEATLLQNNSASDKKTAEAEKATFLTKRQWDREKAC
                     FELGDQVAALEKMKSLISSIKEPSEKQRAIRDLEFLLLTPAEQAKLREERMNRRMPGG
                     PRF"
     gene            complement(748..2529)
                     /locus_tag="PK077_03930"
     CDS             complement(748..2529)
                     /locus_tag="PK077_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23477.1"
                     /translation="MLKQIWRWVSLFPLLHPVWFNLLLLVLAWSLVGVAYQSNDDLVI
                     ASVLDGWGDPSYADAHVIFVNPLLTGLLLKVAPVLGGVSVWPVFLALATLSSGAAIFT
                     MLTAHARKARRYDFNTLVLLLVWLLIMPGFYAALQFSHAAFLIGFTGVLACLKYGSSW
                     RGWCAGVFLCVLGSMVRLDAALVCDAFWGAILLAGSLEGLRPSREKLFAFSRLWLALA
                     CVLISSFSLNEYNKYAHRNVLEGCDVAAWNQARALLSDTCPIRQESAYQCEKYGVFAN
                     DIRMVRMFTNCDMDVFNTDYLERLLLARDGGEFWPRAWARGEKALHLFSWDSVMDLLP
                     CWILFGLACRVSFRRGDSPALVYCVGSLLVLILLYMSSIDRLYFRVVYPTFLIAGLCL
                     LWSDKYRPIRINWTGKGALAGGVPMFCAFSVSLSQACAAVWEANLESFSPDAGRLLCE
                     RVDREPDFLFCVQMSGGTIDDLAPGQSVFRKAWSCSRKNVVTLGGWNFPLKPVQRKLK
                     EMGWAGQSSLNLCRDNVYIVCLTPQGDVSDMDSKFMGMLSRHIWEQHGVRTAWHLDEN
                     IQGVRVYRLRKTWFQPGRKEAASVKEV"
     gene            complement(2658..3002)
                     /locus_tag="PK077_03935"
     CDS             complement(2658..3002)
                     /locus_tag="PK077_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23478.1"
                     /translation="MSEEFYIGLNLFSVFLASCTQVVLKKSAMNDRLTGLAYFVNPAT
                     MAAYSVFLGCCLLTFYCLSHLPMITVNMLECSAYVYILLFDRIFFGKPITMRKVGGNA
                     MIIAGIIVCLNR"
     gene            complement(2995..3333)
                     /locus_tag="PK077_03940"
     CDS             complement(2995..3333)
                     /locus_tag="PK077_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23479.1"
                     /translation="MINYLILHLLFIVYALNTVLGRMAAPYGWTDWRRFALLAGVLSL
                     LGIYAAGWQVMLKKFNLGVAYANRAAVVIWGILLAWLCLGEALSWTLAAGAALVIGGI
                     ILVSTEEAHE"
     gene            complement(3360..4298)
                     /locus_tag="PK077_03945"
     CDS             complement(3360..4298)
                     /locus_tag="PK077_03945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRN23480.1"
                     /translation="MTDPQVQLSIVATVYCTGGYLAEFCSRSFAAALEAGYDPSRTEV
                     VLVNDGCPRDGLEQALKEREKDDRVRVVDLSRNFGHHLAMYVACEEARGERVFLIDSD
                     LEESPEWLVDFSREMDSTGADVVYGVQEKRKGGLFEAVSGELFYTLFNLLSDQQITRN
                     MVTARLMSARFLREMLKFPDREPFFFGLCVSAGFRQVPCRVKKASSSPTTYTFRKKMA
                     LAVNSVVSYSARPLVYISYFGLAVTLVAMLYVAWVVVRQLLYNVAPPGWASSVASIWL
                     VGGLILMSLGVIGIYISKIYKETKHRPAVVVARRYE"
     gene            complement(4295..4795)
                     /locus_tag="PK077_03950"
     CDS             complement(4295..4795)
                     /locus_tag="PK077_03950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN23481.1"
                     /translation="MMKICLVNVSDVDEAMRSRLRVWRNSEKVSPFFLLDQVTEEQHR
                     AWLERNVRGEDARACVIYADQVPLGLVYLPWFDRNTRCGEIGIYLYDRAFRELRPASA
                     AYAGMMELAAGELGLERLYARILEDNAASVRFHERMGFVRVPEKDEVCVKSGERKRVL
                     MYAKAL"
     gene            complement(4792..5931)
                     /gene="rffA"
                     /locus_tag="PK077_03955"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(4792..5931)
                     /gene="rffA"
                     /locus_tag="PK077_03955"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HRN23482.1"
                     /translation="MEIPFNQPHLHGPELDYIRDAIQRAHLCGDGYYTKKCHELLAER
                     TGAGKVLLVHSGTAALEMAALLIDCRPGDEIIMPSYTFVSTANAFVLRGGVPVFVDIR
                     PDTQNIDETLIEAAITDRTRAICVVHYAGVACEMDAIMEIAERHRLYVIEDAAQGVGS
                     CYRGRRLGSIGHLGCYSFHETKNVISGEGGALLVNDPSLMERAEIIREKGTNRARFFR
                     GQVDKYTWVDVGSSYLPGEMTAAYLYAQLEHGQEINGERLKWWNMYHRALAPLEEQGV
                     LRRPCVPEHCTHNAHMYYVLLRSMEERTRLINKLAEHGIKAVFHYIPLHSSPAGMRFG
                     RSAGAMPHTDRTSDTLLRLPLYYDLGEEGCREVLRAGFGLEGVES"
     gene            complement(6053..7174)
                     /locus_tag="PK077_03960"
     CDS             complement(6053..7174)
                     /locus_tag="PK077_03960"
                     /inference="COORDINATES: protein motif:HMM:NF027716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HRN23483.1"
                     /translation="MSSLSDTRPQRIAAIDALRGFDMFFLTGGLALVVAGINLFYDRS
                     PEWLVKHSTHVAWEGFAAWDLVMPLFLFIVGTAMPFSFSRRIGSEPLWKIYLKVARRV
                     VVLFLLGMVVQGNLLSFEPSKMSLYCNTLQAIASGYLIAAICLLHLSVRWQVAATGGL
                     LAVYWLVMKFVSFSDPAVGSCAAGMLEPGRNLALLLDKYLMGNWQDGTNYAWILAQFG
                     FGAMTMLGLLGGQILKRVQGHGKKLAWLLCSGAGCLALGYVWSLDFPIIKRLFTSSMV
                     LWAAGWCYLLLALFYLLTDVLKLNWLTFFFSVIGSNAIFVYIWVSLCPPTSNFSRVLF
                     TGFSGCFGDADRFVFYLCNYALIWGVMYYMYKNRTFIKV"
     gene            complement(7171..7308)
                     /locus_tag="PK077_03965"
     CDS             complement(7171..7308)
                     /locus_tag="PK077_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23484.1"
                     /translation="MKKGIMTELPQRMGFSGNSRLFTEPEKKKVFGKIGVHNAVVGIL
                     P"
     gene            7328..7861
                     /locus_tag="PK077_03970"
     CDS             7328..7861
                     /locus_tag="PK077_03970"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HRN23485.1"
                     /translation="MLCFPRNMTMGNDQSGERDSFRNIETYARLKMDELGLADWQFGW
                     DRAKRRLGVCRLLEKSITISIHFARANLETPHEIRDTILHEIAHALAWTRHGERTHGP
                     RWKQICREIGAVPCASAKPDAIRVTTYKYLLRLKTTGEIVGKYHRRPSFAKYLKRMAL
                     KGRPDTLGQLELAPYEE"
     gene            complement(7944..8507)
                     /locus_tag="PK077_03975"
     CDS             complement(7944..8507)
                     /locus_tag="PK077_03975"
                     /inference="COORDINATES: protein motif:HMM:NF028108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HRN23486.1"
                     /translation="MMEEDWNDTFYDLFREAVGRYHEGHRNVDGFFTDQEIIFLFSIG
                     CRTRELFDFVETYARTGEPSPTTVLLMAAARRDFFLTVQHGQFYQGKPVIGYDLPGFG
                     DELCGLPYLPRLIAKARAKLVGSMGDDIIYCCENDRRFFRDHGNIHPADFLRVVWAAG
                     DSDPKVYDYVRQRTLSVSSAVPDNEQT"
     gene            complement(8504..8926)
                     /gene="atpC"
                     /locus_tag="PK077_03980"
     CDS             complement(8504..8926)
                     /gene="atpC"
                     /locus_tag="PK077_03980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HRN23487.1"
                     /translation="MSMEFKVITPDEVAVTATVEYVYLPGSLGEMGVLEHHTALITSL
                     EPGELRYKPMDGPEESMVVGTGFVQVNGNQVLMVTDLALNSSQIDEGSVERAIQEAQE
                     VLKTRSEMSREEQARFEANLTKQIMMLNFKRKHKSPGR"
     gene            complement(8950..>10032)
                     /gene="atpD"
                     /locus_tag="PK077_03985"
     CDS             complement(8950..>10032)
                     /gene="atpD"
                     /locus_tag="PK077_03985"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRN23488.1"
                     /translation="MKSIHRGAPPLDEQSTSTEVLETGIKVIDLICPFLKGGKIGAFG
                     GAGVGKTVLIMELINNIAKARSGLSVFAGVGERTREGNDLYNEMIESGVINLEKPEES
                     KVALVYGQMNEPPGARLRVALSALTMAEYFRDEEHKDVLLFIDNIFRFSQAGSEVSAL
                     LGRTPSAVGYQPNLAEEMADLQERITSTKHGSITSMQAVYVPADDLTDPAPATTFAHL
                     DSTVVLERSLAAQGLFPAVEPLSSTSKALAPEIVGNEHYEVARGVQMVLQRYKDLQDM
                     IAILGMDELSEEDKLIVSRARKIQRFLTQPFHVAEVFSSIPGAYVPVAETVRGFKEIL
                     EGKLDMVPEDAFFMRGGIDDVLKEAK"
BASE COUNT         2500 a         3100 c         2574 g         1858 t
ORIGIN      
        1 caagcagaac acgggaaaca acgtggcccc cggttttaaa aaagcccttc aaatccagtg
       61 caaatggatg ctgctttgcc tgagaaaagc ggatgcggaa aaaaacgagc gtgaactcaa
      121 cgtttcctcc agcgtcctct ccatgctgga tgaagtggcc gccaacgcca aggacgtagg
      181 cgaacagctt cccatggtaa gcggagccag cgaagtcatc cgcacctatc tgaacattgg
      241 ggactaccgt tcggaaaccc tgccggacaa cctgatggac ctcaatacca tctttgaccg
      301 cgtgctgctg gttccctata aggagaaaaa agatgtggaa aatttccgga agctctggaa
      361 caaacgcatc gccctggaag cgactctgct tcaaaacaac tccgccagcg ataagaaaac
      421 ggcggaagcg gaaaaagcca cttttctgac caaaaggcag tgggatcggg aaaaagcctg
      481 ctttgaactc ggtgaccagg tagcggccct tgaaaaaatg aaaagcctga tttccagcat
      541 caaggagcct tctgaaaaac agcgcgccat ccgcgacctg gaatttctgc ttcttacccc
      601 ggcggaacag gcgaaactcc gcgaagagcg catgaacaga cgcatgccgg gcggcccgcg
      661 cttttaacaa tcgtcgccaa gccccaacct ccctcctttc atcccccgtt gcgtaacgcg
      721 catcggggga tgttacattg cggagagtca cacttccttg acggaggcag cttccttcct
      781 gccgggctgg aaccatgtct tgcgcaggcg gtaaacgcgc acaccctgga tattctcatc
      841 cagatgccag gctgtgcgaa cgccgtgctg ctcccagata tggcggctga gcatccccat
      901 gaacttgctg tccatatcgg aaacgtcccc ttgcggcgtg agacagacaa tgtacacatt
      961 atccctgcac aggttcaggg aggactgccc cgcccacccc atctccttca gtttccgctg
     1021 tacgggtttc aacggaaaat tccagccgcc cagcgtgaca acgttcttcc gggaacagct
     1081 ccaggccttg cggaaaacag actgccccgg ggccagatcg tcaatagtgc cgccggacat
     1141 ctggacgcaa aacaggaaat cgggctcccg gtccacccgc tcgcataaca ggcgcccggc
     1201 atccggggaa aaagactcca gattagcctc ccacacggcg gcgcacgcct gggacaaaga
     1261 aacggagaac gcgcagaaca tgggcacgcc gcccgcaaga gctcccttcc ccgtccagtt
     1321 gatgcggatg ggacgatatt tatccgacca cagcagacac agccctgcaa tcaggaacgt
     1381 tggataaact acgcggaaat acaggcggtc tatgctggac atatacagca gaatcagcac
     1441 cagcagggag cctacgcagt acaccagggc cggagaatcc ccgcgccgga aagaaacgcg
     1501 gcatgccaga ccgaacaaaa tccagcaagg gagaagatcc attacggaat cccatgaaaa
     1561 cagatgcaag gctttctctc cacgggccca cgccctgggc cagaattcac ctccgtcccg
     1621 cgccaacaga agcctttcca gataatccgt gttgaataca tccatatcgc aattggtaaa
     1681 catccggacc atcctgatat cgttggcgaa cacgccgtac ttttcacact ggtaggcgct
     1741 ctcctgcctg atgggacacg tgtccgagag aagggcgcgg gcctggttcc atgccgccac
     1801 gtcacatccc tccagcacat tccggtgggc gtatttatta tattcattca gactgaagga
     1861 cgaaatgagc acacaggcca gagccagcca gagacgactg aaggcaaaca gcttttccct
     1921 ggacggcctg agcccttcca ggctgcctgc caacaggatg gccccccaga aagcgtcgca
     1981 caccagggcg gcatccagcc ggaccatgct gcccagcacg cacaggaata ctcccgcgca
     2041 ccaccccctc caggaagagc cgtacttcaa acacgccagc acacctgtaa atcctatcag
     2101 aaaggcggca tggctgaatt gcagagcggc gtaaaagccc ggcatgatca gaagccacac
     2161 cagcagcaga accagcgtat tgaaatcata tctgcgcgcc ttgcgggcat gcgccgtcag
     2221 catcgtgaaa atggccgctc cgctggacaa cgtggcaagc gccagaaaca caggccaaac
     2281 ggacaccccg ccaaggacag gcgcaacttt caacagaaga ccggtcaaca ggggattgac
     2341 gaaaatgaca tgggcgtccg cataagaggg atccccccat ccgtcaagca cggacgcaat
     2401 caccagatcg tcattggact ggtaagccac gcccacaagg ctccaggcca gaaccagaag
     2461 caacaggttg aaccatacgg gatggagcag aggaaaaagc gatacccatc tccaaatctg
     2521 tttcaacata agcaaattcc ctgacgttac agactgtttt ccctgtccgg ggacgcatac
     2581 taggaacggg gaaaatgaaa gtcaagagcc gatccggatg aacaaaccgg aggggctaaa
     2641 aggctgcaaa ctcttcctta acggttcagg cagacgataa tgcccgcgat aatcatcgcg
     2701 ttgccgccca ctttgcgcat ggtgatcggt tttccaaaaa aaatccggtc aaacaaaagg
     2761 atatacacat atgcggagca ttccagcatg ttcaccgtaa tcatcggcag gtgggacagg
     2821 cagtaaaacg tcagcaggca gcagcccaaa aaaacggaat atgccgccat cgtggccgga
     2881 ttcacgaaat aggcaaggcc ggtcagccgg tcattcatgg cggatttttt caaaaccacc
     2941 tgtgtgcagg aggccagaaa aacggaaaac aggttcagcc caatataaaa ttcttcactc
     3001 atgcgcctcc tccgtggaaa ccaaaataat ccccccgatg accagagccg ctcccgccgc
     3061 cagcgtccag gaaagagcct cccccaggca aagccatgcc agcaggatac cccagatgac
     3121 aacggcggca cggttggcat aagccacacc cagattgaat tttttcagca tgacctgcca
     3181 tcccgccgca taaattccca atagggacag cacgccggcc agcagggcga accggcgcca
     3241 gtccgtccag ccgtaggggg cagccatgcg ccccagcacg gtattcagcg cgtaaacgat
     3301 aaacagcagg tgcagaatca ggtagttgat catgaaagaa ataaagagcc gggaacaatt
     3361 cactcatacc ggcgcgccac caccacggcg ggcctgtgct tggtttcctt gtaaatcttg
     3421 gaaatgtaaa tgccgataac gcccaggctc atcagaatca agcctccgac cagccagatg
     3481 gaggccacgg aagaggccca gccgggaggg gctacattat acaggagctg gcgcacaacc
     3541 acccaggcga catacagcat ggctaccagg gtgaccgcca ggccaaagta ggaaatatac
     3601 accagcggcc tggcgctgta ggaaaccacg gaattcaccg ccagcgccat ttttttgcgg
     3661 aaagtgtagg tagttggaga agaggatgct ttcttcaccc tgcacggcac ctgccggaaa
     3721 ccggcggaaa cgcacagccc aaagaaaaaa ggctcccggt ccgggaattt cagcatctcc
     3781 cgcaggaaac gcgcgctcat cagccgcgcc gtaaccatat tcctggtaat ctgctggtcg
     3841 gaaagcaaat taaacaatgt gtagaacaat tctccggaaa cggcttcaaa caagccgccc
     3901 ttcctcttct cctgcacccc gtacaccaca tccgccccgg tagaatccat ctcacgggaa
     3961 aaatcaacca gccactcggg agattcctcc aaatcactgt caatcaagaa tacgcgctct
     4021 ccccgggctt cttcgcaggc cacgtacatg gccagatgat gaccgaagtt gcgggataaa
     4081 tccaccacgc gcacccggtc gtccttttcc cgttccttca acgcctgttc cagaccgtcc
     4141 cttgggcagc cgtcattcac cagcaccacc tccgtgcggg aggggtcata acccgcctca
     4201 agcgcggcgg cgaaactgcg ggaacagaac tccgccagat aaccgcccgt acaatacacg
     4261 gtagccacaa tggacaattg aacctgtgga tcagtcataa cgcttttgca tacatgagaa
     4321 cgcgtttcct ttccccgctt ttcacgcaca cctcgtcctt ttccggaaca cggacaaaac
     4381 ccatgcgctc atgaaaacgc acggaagccg cattgtcctc cagaatccgg gcatataacc
     4441 gttccaggcc cagctcccct gcggccagct ccatcatccc ggcatatgcg gcggaagccg
     4501 ggcgcagttc cctgaatgcc ctgtcgtaaa gataaatgcc tatctctccg caacgcgtat
     4561 tccggtcaaa ccagggcagg taaaccagac ccagcggaac ctggtccgca taaatcacgc
     4621 aggcgcgggc atcctccccg cggacgttcc tttccagcca cgcccggtgc tgctcctcag
     4681 ttacctggtc caacaaaaaa aacgggctga ccttttcgga attgcgccac acgcgcaggc
     4741 ggctgcgcat ggcttcatcc acatcagaca cgttaaccag gcaaattttc atcatgattc
     4801 aactccctcc agcccgaagc cggcccgcaa cacctcccgg cagccttctt cccccaaatc
     4861 gtaatacaaa ggaagcctca gcaacgtatc gctggtacgg tccgtatgag gcatggcccc
     4921 ggcagaacgc ccgaatctca tcccggcagg ggaggaatgg aggggaatgt agtggaacac
     4981 cgctttaatc ccgtgctccg ccagcttgtt gatcaagcgc gtgcgctcct ccatggaacg
     5041 gagcagcacg taatacatgt gggcgttatg cgtgcaatgc tccggaacgc acggacgccg
     5101 cagcaccccc tgttcttcca atggtgccag agcgcggtgg tacatattcc accacttcaa
     5161 cctttccccg ttgatctcct gcccgtgttc cagctgggca tacaaataag cggcggtcat
     5221 ctctccgggc agataggagg aacctacgtc cacccaggtg tatttatcca cctgcccgcg
     5281 gaaaaacctg gcacggttgg tccctttttc ccggataatc tccgcgcgtt ccatcaggga
     5341 ggggtcattc accagcaggg ctcctccctc tccggaaatg acattcttgg tctcgtggaa
     5401 actgtaacag cccaaatggc cgatagagcc gagccgacgc cctctgtagc aggaaccgac
     5461 gccctgggcg gcgtcttcaa tcacatacag gcggtgcctc tcggcaattt ccatgattgc
     5521 atccatttca caagccacac ccgcgtaatg aaccacgcaa atggcgcggg tgcggtccgt
     5581 gatggccgct tctatcagcg tctcgtcaat attctgcgta tccggcctga tatccacgaa
     5641 tacgggaacg ccgccgcgca gcacaaacgc gttcgccgtg gagacgaacg tataggaagg
     5701 catgataatc tcgtcgcccg ggcggcagtc gatcaacagg gccgccatct ccagtgcggc
     5761 ggttccggaa tgaaccagca gaacctttcc ggcgccggtg cgttccgcaa gcagttcatg
     5821 gcattttttc gtgtaatagc cgtctccgca taaatgggcg cgctggatgg cgtcccggat
     5881 ataatccaat tcgggaccgt gcaggtgagg ttggttgaaa ggaatctcca tggcttcaga
     5941 aaagaccggc ccataacgga cgcccggcgc aggcatgccg atcccgtgcg ggaggcgttc
     6001 cccgcctccc gcacggggag accctactat gaacggaggg ctgaaaacaa gcctagacct
     6061 tgataaaggt ccggtttttg tacatgtaat acatcacgcc ccaaatcagg gcgtaattgc
     6121 agagataaaa gacaaacctg tccgcatccc cgaaacaccc gctaaacccg gtaaacaata
     6181 cccgggagaa attgctcgta ggggggcaca gagataccca tatgtacacg aaaatggcat
     6241 tgctccctat tacggagaaa aagaatgtca gccagttcaa tttcagcaca tccgtcagca
     6301 ggtagaacag ggccagcagc aaatagcacc atcccgccgc ccacaacacc atggaactgg
     6361 tgaacaaacg cttgattatc gggaaatcca ggctccagac ataccccagc gccaggcagc
     6421 ccgcgcccga acataacagc cacgccagct ttttcccatg cccctgcacc cgcttcagaa
     6481 tctggccgcc cagcagaccg agcatggtca tggcgccaaa accgaactgc gccagaatcc
     6541 atgcataatt cgttccatcc tgccagtttc ccatcaggta tttatccagc agcagggcca
     6601 gatttctccc cggttcaagc attcctgccg cacaggaacc caccgctggg tcagaaaagg
     6661 aaacaaactt catgaccagc cagtacacag ccagcagccc ccccgttgcc gctacctgcc
     6721 accggacgga cagatgaaga aggcaaatgg ccgcaatcag gtagccggaa gcgatggcct
     6781 ggagcgtatt gcagtacagg gacattttgg acggttcaaa actcagcaga tttccctgca
     6841 ccaccatgcc cagcaaaaaa agcaccacta cccgcctggc aactttcagg taaatcttcc
     6901 acaggggttc cgaaccgatg cgcctggaaa aggaaaacgg catggccgtt cccacaataa
     6961 ataaaaagag gggcatcacc agatcccagg cggcgaatcc ctcccaagcc acgtgcgtgc
     7021 tgtgcttcac cagccactcg gggctccggt cgtaaaaaag attgatgccg gccacaacca
     7081 gagccaggcc cccggtcagg aaaaacatgt caaatccccg cagggcgtca atggccgcaa
     7141 tcctctgcgg cctcgtatcg gaaagtgaac tcatggcaat atcccaacta cggcattatg
     7201 tacgccaatc tttccaaaga ctttcttttt ctcaggctcc gtaaataaac ggctgtttcc
     7261 ggaaaaaccc atcctctgcg gcaactccgt catgatgcct ttcttcatat tccctgcccc
     7321 gcatttcatg ctatgctttc cccgcaacat gaccatgggg aacgaccaat ccggcgaacg
     7381 cgacagcttc cggaatattg aaacttacgc ccgcctgaaa atggatgaac ttggtctggc
     7441 agactggcaa ttcggctggg accgggccaa aagaaggctg ggcgtatgcc gcctgctgga
     7501 aaaaagcatt acgatttcca tccacttcgc ccgcgccaac ctggagaccc ctcatgaaat
     7561 ccgtgacacg attctgcatg agatcgccca cgccctggca tggacccgcc acggagaacg
     7621 cacccacgga ccgcgatgga aacaaatttg cagggaaata ggggctgtcc cgtgtgcctc
     7681 cgccaaaccg gacgccatcc gcgtcaccac gtacaaatat ctcctgcgtc tgaaaacgac
     7741 gggagaaatc gtcggcaaat accaccgccg cccctctttc gccaaatacc tcaagcgcat
     7801 ggccctgaaa ggccgccccg atacgctggg ccagctggag ctggcgcctt atgaagaata
     7861 acgtccacaa aagctgaaag aaacgagccg tttccggtat tctccagcat caacgatcat
     7921 cccattccgg gcatttccat tttttacgtc tgctcattat ccggaacagc agaggaaaca
     7981 ctcaaggtgc gctggcgtac gtaatcatac accttcgggt cgctgtctcc ggctgcccag
     8041 actacccgca ggaagtcggc gggatgaatg tttccatggt cccggaagaa acggcggtca
     8101 ttctcacagc agtaaatgat gtcatccccc atggaaccga cgagcttggc ccgcgccttg
     8161 gcaatcaggc gcggaaggta ggggagaccg cagagttcgt cgccgaaccc cggcaaatca
     8221 tacccgatca caggtttccc ctggtaaaac tgcccgtgct ggacagtcag gaagaagtcc
     8281 cggcgcgccg ccgccatcag tagaaccgtg gtgggggaag gttctcccgt gcgggcatag
     8341 gtttctacaa aatcaaacaa ttcccgcgtg cggcagccga tggaaaacag aaagataatt
     8401 tcctgatccg taaagaagcc gtccacattc ctgtggcctt catggtagcg ccccaccgct
     8461 tcgcggaaaa ggtcgtaaaa tgtatcgttc caatcttcct ccatcatcgg cccggcgatt
     8521 tgtgtttgcg cttgaagtta agcatcatga tctgcttggt cagattggct tcaaaacggg
     8581 cctgttcctc gcgggacatt tccgaacgcg tcttgagcac ctcctgcgct tcctgaatgg
     8641 cccgctccac gctgccttca tcaatctggg aggaattgag cgcgaggtcg gttaccatga
     8701 ggacctggtt tccattgacc tgcacaaagc cggtgcctac caccatgctt tcctccgggc
     8761 cgtccatggg cttgtagcgg agctcccccg gttccaggga ggtaatcagg gccgtatgat
     8821 gttccagcac gcccatctcc ccgaggctgc cgggcagata aacgtactct acagtagcgg
     8881 taaccgccac ttcatccggg gtaatgactt tgaactccat gctcatggtt cggaacggcg
     8941 ggaaaagggt tatttggctt ccttgagaac gtcatcaatg ccgccgcgca tgaagaaggc
     9001 gtcctccgga accatatcca gcttgccttc caggatttcc ttgaaccccc ggacggtttc
     9061 cgccacgggc acataggcgc cgggaatgct ggaaaagact tccgccacat ggaacggctg
     9121 ggtcaggaag cgctgaatct tgcgagcgcg gctcacgatg agcttgtctt cttccgaaag
     9181 ttcgtccatg cccagaatgg caatcatatc ctgaaggtcc ttgtagcgct gcagcaccat
     9241 ctgcacgcca cgagccactt cataatgctc attccccaca atttccgggg ccagggcctt
     9301 ggatgtggaa gacagcggct ccaccgccgg gaagagcccc tgggccgcca gagagcgttc
     9361 cagcaccacc gtggagtcca ggtgggcgaa ggttgtggcc ggggccgggt ccgtcaagtc
     9421 gtccgcagga acatacacgg cctgcatgga tgtgatggaa ccgtgcttgg tggaagtgat
     9481 gcgttcctga agatccgcca tttcctccgc caggttcggc tggtaaccca cggcggacgg
     9541 cgtgcggccc agcagggcgg acacttccga ccccgcctgg gagaaacgga aaatattgtc
     9601 aatgaacagc agcacgtcct tatgctcctc gtcacggaag tattccgcca tcgtcagggc
     9661 ggacaaggcc acgcgcagac gggcgccggg aggttcattc atctgtccgt acaccagcgc
     9721 caccttggat tcttccggtt tttccaggtt gatgaccccg gactctatca tttcattgta
     9781 caggtcgttc ccttcacggg tgcgttcccc caccccggca aagacggaga ggccgctgcg
     9841 cgccttggca atgttgttaa tcaactccat gatgagcacg gttttgccta cccccgcacc
     9901 cccaaaggcg ccgattttgc cgcctttcag gaaggggcag ataaggtcaa tgaccttgat
     9961 gcccgtttcc agcacttccg tgctggtgga ttgttcgtcc agcggcggag cgccgcggtg
    10021 gatgctcttc at
//
LOCUS       DAOMSU010000058         9915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_429_length_9915_cov_2.886410, whole genome shotgun sequence.
ACCESSION   DAOMSU010000058 DAOMSU010000000
VERSION     DAOMSU010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_429_length_9915_cov_2.886410"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="PK077_03990"
     CDS             complement(<1..1563)
                     /locus_tag="PK077_03990"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRN23489.1"
                     /translation="MEEKNYLVSGMHCAGCAAKVERAVEGLEGVERVELNLLTGKMTV
                     LFEKPDSPAMERIAPVVEKAGFRLADWKEEPLAGTEREERLEQEETGGSRLVWSILLL
                     VPLMYLSMGPMWHWPVPEGSWGLWMNWWTQGILAGAVLWLNRHYLINGVRQLVALSPN
                     MDSLIAIGSGSAFLYGLYLLVAGMWQGFSVEGMELYFESAAMIVTLISLGKYLERRSY
                     RKTNAAVKGLVKLVPQEALVWHDGAERAVPLDEIRSGDLVVVKTGQRIPVDGMIEEGQ
                     AALDESDLTGESMPVDRAAGDRVISGTFNRAGYLKIRAERVGGDSTLARMIRLVEQAS
                     QSKAPIARLADRVCYFFVPAVIAVALVSLVGWMAAGEGVSFALARAIAVLVISCPCVL
                     GLATPIAIMVGTGRGARLGILCKSAGALEALSRVDTVVFDKTGTLTEGEPRVVAVLPE
                     EGMDADALVEMAAALEQGSEHPVGKAVYEHARLLNLPVRAVADLSVVPGRGIAGTVDG
                     VPYAVGNSGFMQD"
     gene            1743..2684
                     /locus_tag="PK077_03995"
     CDS             1743..2684
                     /locus_tag="PK077_03995"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HRN23490.1"
                     /translation="MNIPPLFNILASAALLSTPLHAQLYTLHGDGVKKTERVVEKADY
                     SDYELVWHDEFDKDGRPDPAKWNYEHGFVRNKEAQWYQPENAYCKDGMLIIEGRKEKH
                     ANPHYDPEGKNWQTTRKEAEYTSASLTTRSKFSWLYGRFEIRARFSPREGMWPAFWTL
                     GVKEKWPMCGEIDIMEYYQSTYLANLCWGSPKKHVGKWSTTYTPLKWLQEKHADWADS
                     FHVFRMDWDEKEVRLYADDILLNRTPLDNTVNASYKEVANPFRQPHFIILNLALGATG
                     GDLDKLPLPQKYEIDYVRIYQKKDSPHKGTIPYRAEK"
     gene            complement(2790..3356)
                     /gene="efp"
                     /locus_tag="PK077_04000"
     CDS             complement(2790..3356)
                     /gene="efp"
                     /locus_tag="PK077_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRN23491.1"
                     /translation="MAKVPVINLRKGHAVNYNNDVCVVVSMEHKCPPRMASYVQMSIR
                     SISTKKVYNLRLTSNESLEGVNLAREEYEFSYIDGMGYHFMNPDTYEDITVSPEIVEP
                     VKDYLMEGNIYILLFTDETVVSVELPAAITMEVAEAPEGVKGDSANNVYKSATMTTGL
                     VVQVPLFIKPGEKISVKTEDGSYLGRVN"
     gene            complement(3480..3938)
                     /locus_tag="PK077_04005"
     CDS             complement(3480..3938)
                     /locus_tag="PK077_04005"
                     /inference="COORDINATES: protein motif:HMM:NF040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23492.1"
                     /translation="MKEEAIKAPGSTIKQYSIMLQNRVGALTALLGLLDMHGIFCLGF
                     SMQDCHEATIARLIVSDPERTSEIFLEKGICYTESGVLVAVLRHGPADLKKCLDALYA
                     AEMNVNFLYPLLPCVGRGSLVALHVEDLNFGRTVLNSSGIKVLFQQDLSR"
     gene            complement(3966..5222)
                     /locus_tag="PK077_04010"
     CDS             complement(3966..5222)
                     /locus_tag="PK077_04010"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HRN23493.1"
                     /translation="MNTEEKLNQYVLHSYGRYPMAAVRGEGSRLWDSTGKCYLDFCAG
                     IATCVLGHCHPAVTQALQKQAATLVHCSNLYQIPQQADLAEFIVTKCVERPGKVFFAN
                     SGAESNDGLIKVARRYGHRKPQPDGKPRYEVLTFNKSFHGRTLGSMSATGQDKIKIGF
                     DPMLPGFRHLPFNDLETAREAIRPETVAFMLETVQGEGGVNCVTPEFLRGLAQLCREH
                     DLLLLMDEVQCGFGRCGDIMGWRAVAPDVEPDGISWAKALGGGFPIGGFWISDRAIDG
                     QGTELSSIMDPGSHGSTYGGNPLGTAVGLAVLREVVSQGLPERARRLGAEIRREIESW
                     NLPVVQGVRGLGLLLGIGLNPEMVAAPEGKTIASVVVEALRQEGLLSVPAGPETVRLL
                     PPLNVSEEEIQQALAIIRRVLKTYAK"
     gene            5411..5824
                     /gene="mscL"
                     /locus_tag="PK077_04015"
     CDS             5411..5824
                     /gene="mscL"
                     /locus_tag="PK077_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRN23494.1"
                     /translation="MAHFFNLKGFLEEFKAFALKGNVIDLAVAVVIGGAFNKIVSVFV
                     SSIITPIIGYLTSGVNLAYLVLKLGDVELKYGELLQATIDFLIIAFSVFVAIKLIGAL
                     RRKSEEAPAAPAPKPDDVVLLEQIRDILQKQADAK"
     gene            5914..7059
                     /locus_tag="PK077_04020"
     CDS             5914..7059
                     /locus_tag="PK077_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HRN23495.1"
                     /translation="MTSPLRLLAFALFLAPSLLPAAENQRFILERPYPVTEIPEPDRS
                     LPVTNVILMIGDGMGIHHLSAAWAANRGRLFIENCPVTGISKTWCADKLVTDSAAAGT
                     AMATGTKTLYHRVAVCPRGNKLDSLVDKAADMGKSTGVIATCELNDATPASFCANNEK
                     RSDAYGIIGDYPCSRADFIFGGGGRYFTQRPDGRDIFQEMQAQGYRVARTWEEAGALP
                     PGKTLAVTAPGNLPPPSKRGDVFRQAALKALDTLSRNPKGFFLMIEGSNIDKAAHSNN
                     LEEMMEELLDFDRAAGAVLDWASKHPGTLVVITADHNTGAFALTGGSRERGEITARFG
                     SGSHDGVAVPVYAFGAGSGAFTGIYENTDIFNKIMNALKKGADKPVR"
     gene            complement(7068..8258)
                     /locus_tag="PK077_04025"
     CDS             complement(7068..8258)
                     /locus_tag="PK077_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN23496.1"
                     /translation="MNEAASPRIRKTMVFVLLALFLGQFGSGVYDLIFSNFLRDAQHL
                     DVEMRGLIEFPRELPGILSLFAVSLLFMFNEVRVAGVACLLMFGGMYALAFCSAGTSL
                     WSLSAWILTVSLGQHILMGMIDTIVIHTARPENRSLRLGQMKALGTAAALLGALCVWI
                     KWKFNQSFAVDFFVTGGVCLLAFLFLSRVKTPVFPQRRGWRQCFVFKRRYAVYYGLEI
                     LHGIRKQLYLTFGFWLMVSTLDQSPGYIGKTLLMAGVIGLVTQPLIGWSIKRYGERKV
                     TIFDSVALSLLCLAYAFAPELLPLHWAVGVVTACFVLDNLLFALGMARSTYVARICEK
                     PEDITPSIYTGLAINHVASIAYGVLGGVIWMNTGGPQAVFLVGGVATAGAGLVARRMD
                     RPQL"
     gene            8388..>9915
                     /locus_tag="PK077_04030"
     CDS             8388..>9915
                     /locus_tag="PK077_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRN23497.1"
                     /translation="MISKCTFSAMVFSLFSVCWGAPSAPVLEAPHIIPLPAAMRVQTG
                     ESGFSLKNGVRLPEENPLSRQAERIFRDNGINTVLVKNKADIIFTEDTSLGREGYRLA
                     VTPDSISIASASVNGALYALQSLVQSVAADRNGDPALPRLDVEDRPRFSWRGLMMDSC
                     RHMMPVRDIKKILDLMERYKFNTLHWHLTDDQGWRLPVSKYPRLTTVGGTRAQSPVIG
                     NRNKADGTPYSGHYTADEIRDVVRYAKNRGITVIPEVEMPGHASAAIAAYPELGNTDI
                     PGYAPKVQETWGVLPYTFAPTEKTFLFLEDVIDEVCALFPDSPYIHIGGDEAPKNQWK
                     QSPAAQQVMKDNGLANEHELQSYFIRRVEKIINSRGKRLIGWDEIQEGGLSPTATMMV
                     WRSWMPNSARHALEQGNDVVMTPNSHLYFDYDQGPGKPAAPEYETINNNHLTWQHVYG
                     LEPVPQGTPREREKQVLGCQANIWTEYIPNLPKWEYQVFPRALALAEVAWTPQELKNE
                     K"
BASE COUNT         2280 a         3086 c         2623 g         1926 t
ORIGIN      
        1 atcctgcatg aatccggaat tgcccaccgc ataaggcacc ccatccaccg ttccggcaat
       61 gccgcggcca ggaaccacgg ataaatccgc cacggcgcgg acaggcaaat tcagaagcct
      121 ggcgtgttca taaacagcct ttcccacagg atgttccgaa ccctgttcca aagccgccgc
      181 catttccacc agcgcgtccg catccatgcc ttcctccggc agcacggcca ctacccgcgg
      241 ctccccttcc gtcagggtgc ccgtcttgtc aaaaacgacg gtatccacgc ggctcagggc
      301 ttccagagct ccggcggact tgcacaggat gcccagccgc gcgcccctgc ccgtccccac
      361 cataatggcg ataggcgtag cgagccccag gacacagggg caggaaatca ccagcaccgc
      421 aattgccctg gcgagggcga aggaaacccc ctccccggca gccatccacc caaccagcga
      481 taccagggca acggcaatga ccgcagggac aaagaaatag catacccggt ccgccagccg
      541 cgcaatgggc gccttggact ggctggcctg ttccaccagg cggatcatgc gggccagcgt
      601 ggaatccccc cccacgcgtt ccgcgcggat tttgagatac ccggcccggt tgaatgttcc
      661 gctgatcacc cggtcccccg ccgccctgtc caccggcatg ctttcccccg tcaggtccga
      721 ttcatccaga gccgcctgcc cctcttcaat catgccgtcc accggaatcc gctgcccggt
      781 tttcaccacc accaggtccc cggaacgaat ttcatccagc ggaacggcgc gctccgcgcc
      841 gtcatgccat accagcgcct cctgggggac cagcttcacc agccctttta cggcggcatt
      901 cgttttccga taagagcggc gttcaaggta tttccccagg gaaatcaggg tgacgatcat
      961 ggccgcagat tcaaaataca gctccattcc ctccacactg aaaccctgcc acatccccgc
     1021 aaccagcaaa tacagcccat acagaaaagc ggagccggaa ccgatggcga tcagggaatc
     1081 catattggga gacagcgcca caagctgccg cacgccgttt atcaaataat gccgattcag
     1141 ccacaggacg gcgcccgcca gaatgccttg tgtccaccaa ttcatccaca ggccccaact
     1201 cccttccggg acgggccagt gccacatggg gcccatggac aaatacatca aaggaaccag
     1261 cagcagaatg gaccaaacga ggcgggagcc gcccgtttcc tcctgttcca gcctttcctc
     1321 cctctccgtt ccggcaagcg gctcctcctt ccagtccgcc aggcggaaac cggctttctc
     1381 caccaccgga gctatccgct ccatggcagg ggaatcaggt ttttcaaaca agacggtcat
     1441 cttccccgtc agcaaattca gctctacgcg ctccaccccc tccaatcctt ccacggcgcg
     1501 ctccaccttg gcggcacacc cggcgcaatg cataccggaa acaagatagt tcttttcttc
     1561 cattgtcatt aaaacggaat gtttgatgtt ccgggtccag acaagacccc atactgttag
     1621 ccgaacctaa cacatttatt ccgttcctca agccgctttc gcggcttcag ccccctctga
     1681 taaaaaatga caaaaaaacg cccgcggccc cgtaccagta acaaacacct ttactttcct
     1741 ccatgaacat tcctcctcta ttcaacatcc tggccagcgc ggccttgttg tccacccccc
     1801 tccacgccca gctttacacc ctccatggtg acggcgtcaa aaaaacggag agagtcgtgg
     1861 aaaaagcgga ttattccgat tatgaactcg tctggcacga cgaattcgac aaagacggcc
     1921 gtccggaccc tgccaaatgg aattacgaac acggtttcgt ccgcaataag gaagcgcaat
     1981 ggtaccagcc ggaaaacgcc tattgcaaag acggcatgct gattattgaa ggcaggaagg
     2041 aaaaacatgc caacccccat tacgatccgg agggaaaaaa ctggcagacg acacggaaag
     2101 aggcggaata cacctctgcc tccctgacga cgcgcagcaa attttcctgg ctgtacgggc
     2161 gatttgaaat ccgggccaga ttcagcccgc gggaaggcat gtggcctgct ttctggacct
     2221 tgggcgtcaa ggaaaagtgg cccatgtgcg gggaaataga catcatggag tactaccagt
     2281 ccacctatct ggccaatctc tgctggggtt cccccaaaaa acacgttggc aaatggagca
     2341 ccacttacac gccgctcaaa tggctccagg aaaaacacgc ggactgggcg gacagcttcc
     2401 atgtcttccg tatggactgg gatgaaaagg aagtgcgcct gtatgcggac gatattctgc
     2461 tgaaccggac tcccctggat aataccgtca acgcctctta caaggaagtc gccaatccct
     2521 tccggcagcc tcacttcatc atcctgaacc tagccctggg cgccaccggc ggagatctgg
     2581 acaaactgcc gctgccccag aaatacgaaa tagactacgt tcgcatttac cagaaaaagg
     2641 attcccccca taaaggaacc attccatacc gcgcggaaaa gtaaccgccc tcttccggaa
     2701 acaggaaaaa gggcagggat tgcggcatga aaaaagcccc atccggacaa accggacggg
     2761 gctcttcaaa gaacagaaag aggggcaact cagtttacgc ggcccagata ggaaccgtct
     2821 tccgtcttta cggaaatctt ttcaccgggc ttgatgaaaa ggggcacctg caccaccagt
     2881 ccggtagtca tcgtggcgga tttgtaaaca ttgttcgcgc tgtcgccctt gacgccttca
     2941 ggagcttccg ccacttccat ggtgatggcg gcggggagtt ctacggaaac gaccgtttca
     3001 tccgtgaaaa gcaggatgta gatattgcct tccatcagat aatccttgac gggttccacg
     3061 atttccggag acacggtgat gtcttcgtat gtatccggat tcatgaagtg ataacccatg
     3121 ccgtcaatgt agctgaactc atattcctca cgggcgaggt tgacgccttc cagggattca
     3181 ttggaagtca ggcgaaggtt atacaccttt ttagtggaaa tgctgcggat gctcatttgc
     3241 acataggaag ccatgcgggg ggggcacttg tgctccatgc tgactacaac gcaaacgtca
     3301 ttgttgtaat tgaccgcgtg tcccttgcga agattgatta caggtacttt tgccatgcgg
     3361 cccgtaccat accatgtttt cccctcccgt caaggcgaaa aaaccgggca accccttgag
     3421 gcgcttggag aaaacgctcc tgaacagggc agcggggaag aaagggaaaa cggctgttcc
     3481 taccggctga ggtcctgctg gaacaggact ttgatgccgc tggaattcag cacggtgcgc
     3541 ccgaagttga gatcctccac gtgaagagcc accagagacc cccgccccac gcaaggcagc
     3601 aggggataga gaaagttgac gttcatttcc gcggcataca gcgcatccag gcattttttg
     3661 aggtctgcag ggccatggcg gagcaccgcc accagcaccc cggactccgt atagcaaata
     3721 cctttctcca gaaaaatttc cgacgtcctt tccggatcgc tgacaatcag gcgcgcaata
     3781 gtcgcctcat ggcagtcctg catgctgaat cccaaacaga aaatgccgtg catgtccagc
     3841 aggccgagga gggcggtcag ggcccccacc cggttctgga gcatgatgga atattgcttt
     3901 atcgtgctgc cgggggcttt aatggcttct tccttcataa caataaagca aataagggct
     3961 gtattctatt tggcgtacgt tttaagcacg cgcctgatga tggccagagc ctgctggatt
     4021 tcctcttcac tgacattcag cggcggcagc agacggacgg tttccggccc ggcgggaacg
     4081 gaaagcaacc cttcctgccg cagggcctcc accactacgg aggcgatcgt cttgccctcc
     4141 ggcgcggcca ccatttccgg gttcaggcca atgcccagca acaggcccag gccacgcaca
     4201 ccctgaacaa caggcaggtt ccaggattct atttccctgc gaatttccgc gcccaggcgc
     4261 cgcgcccgct ccgggagccc ctgggaaaca acttcccgca gcacggccag gcctaccgca
     4321 gtgccgagcg ggttgcctcc atacgtggag ccatgggagc cgggatccat gatggaggaa
     4381 agctccgtac cctggccgtc aatggcgcgg tcggaaatcc agaagccgcc gatggggaaa
     4441 ccgccgccca gagccttggc ccaggaaatg ccgtccggtt ccacatccgg agctacggcg
     4501 cgccatccca tgatgtcgcc gcagcggcca aagccgcact gcacttcatc catcagcaga
     4561 agaagatcgt gctctctgca taactgcgcc aggccccgca gaaattccgg agtcacgcag
     4621 ttgacccctc cttctccctg gacggtttcc agcatgaagg ccaccgtttc cggcctgatg
     4681 gcttcgcggg ccgtttccag atcgttgaaa ggcaaatggc ggaatcccgg cagcatgggg
     4741 tcaaagccga ttttgatttt atcctgcccc gtggcggaca tgctgcccag cgtcctcccg
     4801 tggaaggatt tgttgaacgt aagcacctca taacggggct tgccgtccgg ctggggcttg
     4861 cggtgcccgt aacggcgcgc tactttgatt aaaccgtcat tggactccgc accgctgttg
     4921 gcgaaaaaga cttttcccgg gcgctccacg catttggtca cgatgaattc cgccagatcc
     4981 gcctgctggg ggatctggta caggttggag cagtgcacca gggtggcggc ctgcttttgc
     5041 agggcctgcg tcaccgcagg atggcagtgc ccgagcacgc aggtcgcaat gccggcacag
     5101 aagtccagat agcactttcc cgtgctgtcc cacagtcggg agccttctcc gcggacggca
     5161 gccatgggat aacggccata ggaatggagt acgtactggt tgagcttttc ttctgtgttc
     5221 ataatttaag gtaatatggg cgtagtttgc tcttctggcc ccgtctgtaa atgatttttt
     5281 tgggaaccac gtcatgattg ccgcatttta ccccggaaga cagtcacatc tttgtccgcc
     5341 gctcattcat ttttcttttc ttttatcatt ttaacggcag aataaccacc gcaaccagca
     5401 ataatccact atggctcatt ttttcaacct caaaggcttt ctggaagaat tcaaggcatt
     5461 cgcccttaaa gggaacgtta ttgacctggc ggtggccgtc gtcatcggcg gcgcattcaa
     5521 taaaatcgtc tccgtcttcg tctccagcat tatcaccccc atcatcggtt accttacttc
     5581 cggggtcaac ctggcatacc tggtgctgaa actgggcgat gtggaattaa aatacggtga
     5641 attgctccag gcgaccattg acttcctcat catcgccttc tccgtctttg tagccatcaa
     5701 gctgatcgga gccctgaggc gcaagagcga agaagctccg gccgcccctg ctcccaagcc
     5761 ggatgacgtc gtccttctgg aacagatccg ggacatcctg caaaaacagg cggacgccaa
     5821 ataagttctt ttgaacggtt catgccggtt ccatgcggga ccggcatgtt ttttcctctt
     5881 tacttgttcc gccgcaaacg ggaaagtgat tgcatgactt ctccgctgcg ccttttagcc
     5941 tttgccctct ttcttgcccc gtccctgctt ccggcggcgg aaaaccagcg cttcattctg
     6001 gaacgcccct atccggtgac ggaaatcccg gaacctgacc gcagccttcc cgtcaccaac
     6061 gtgattctga tgattgggga cggaatgggc attcatcacc tttctgcggc atgggcggcc
     6121 aacagaggcc gcctgtttat tgaaaactgt cctgttacgg gcatttccaa aacatggtgc
     6181 gcggacaagc tggtcacgga ctccgccgct gcgggcaccg ccatggcgac aggaaccaag
     6241 acgctttacc atcgggtcgc cgtatgcccc aggggcaaca agctggattc cctggtggac
     6301 aaggctgcgg acatgggcaa atccaccggc gttatcgcca cctgcgagct gaatgacgcc
     6361 accccggcat ctttctgcgc caataatgaa aaaagatcgg atgcctacgg catcattggg
     6421 gattacccct gttcccgcgc cgatttcatc tttggcggcg gcggcagata cttcacgcag
     6481 aggccggacg gcagggacat cttccaggaa atgcaggcgc agggataccg cgtcgcccgc
     6541 acctgggaag aagccggtgc cctgcccccc ggaaaaacgc tggccgtcac cgctcctggg
     6601 aatcttcccc ctccctccaa gcggggcgac gttttccgcc aagccgcact gaaagccctg
     6661 gatacccttt cccggaatcc caaggggttt ttcctgatga ttgaagggtc caacattgac
     6721 aaggcagccc acagcaacaa cctggaagaa atgatggaag aactccttga ttttgaccgg
     6781 gctgcaggag ccgtgcttga ctgggcctcc aaacaccccg gcaccctggt cgtcatcacg
     6841 gcggaccata acacaggcgc gttcgcgctc acgggaggca gccgggagag gggggaaata
     6901 acggcccggt tcggttccgg cagtcatgac ggcgtggctg ttccggtgta cgccttcggc
     6961 gcgggaagcg gcgcttttac cggcatttat gaaaatacgg atatcttcaa caagattatg
     7021 aacgccctta aaaagggtgc ggacaaaccc gtgcgctagg cgcccggtca aagctgcggc
     7081 ctgtccatgc gccgcgccac cagcccggcc ccggcggtag ccacgccgcc taccaggaac
     7141 accgcttgcg ggccgcccgt attcatccag atcacgccgc ccagcacgcc gtaagcgata
     7201 gaagccacat gattgatagc cagcccggtg taaatgcttg gcgtaatgtc ttccggcttc
     7261 tcgcaaatgc gggccacgta ggtgctccgg gccatgccca gggcgaacag caggttgtcc
     7321 aggacaaagc aggcggtcac tacccccacg gcccaatgca ggggcagcag ctccggtgcg
     7381 aaggcgtatg ccaggcacag gagggacaac gccacgctgt cgaaaatcgt caccttccgt
     7441 tccccgtagc gcttgatgct ccagccaatc aggggttgag taaccagccc gatgacgccg
     7501 gccatgagca gggtttttcc gatatatccg ggagactggt ccagcgtgct gaccatcagc
     7561 cagaaaccaa aggtcagata aagctgcttg cggatgccgt gcagaatttc caatccataa
     7621 taaacggcat acctgcgctt gaagacgaaa cattgcctcc agccgcgccg ctgcgggaag
     7681 acgggcgtct tcacccggct cagaaacagg aacgccagca ggcacacgcc ccccgtgacg
     7741 aaaaagtcca cggcaaaaga ctggttgaat ttccacttga tccacacgca cagcgccccc
     7801 agcagcgcgg cagccgttcc cagagccttc atctggccaa gccgcaggct gcggttttcc
     7861 ggccttgccg tatgaatgac aatggtatca atcatgccca tcaggatatg ctggcccagg
     7921 ctcaccgtca gaatccatgc ggacaggctc cacaggctgg ttccggcgct gcagaaagcc
     7981 agcgcgtaca tgccgccgaa catcagcagg cacgccaccc cggccacgcg gacctcatta
     8041 aacatgaaaa gcaggctgac cgcaaaaaga gacaggatgc cgggcaattc ccgggggaat
     8101 tcaatcagtc cgcgcatctc cacgtcaaga tgttgggcat cacgcaggaa attgctgaaa
     8161 atgagatcat acacaccgct tccgaactgg cccagaaaaa gcgccagcaa cacaaatacc
     8221 atcgtcttcc tgatgcgggg ggaagcggct tcattcatag cgcgggaata ctctcacagg
     8281 cacccggatg gaacaaccgc tattttcatg tgccggtttt ctctgcattt gcaaaaataa
     8341 cctcgaaacg gaattttccc ggaaaatgcc gcgtatttac gtacgctatg atttccaagt
     8401 gtaccttttc tgccatggtt ttcagcctgt tttctgtttg ctggggcgct ccgtccgctc
     8461 cggttctcga agcgccccat atcattcccc tgcccgccgc catgcgcgtc caaaccggag
     8521 aaagcgggtt ttccctgaaa aacggcgtca gactcccgga agaaaatccg ctttccagac
     8581 aggcggaacg gattttccgc gataatggaa tcaatacggt tctggttaaa aacaaagcgg
     8641 acatcatctt tacggaagac acgtccctgg gcagggaagg ctaccgcctt gccgtaacgc
     8701 cggattccat ttccattgcc tccgcttctg tgaatggcgc tttgtatgcc cttcaatccc
     8761 ttgtgcaaag cgtcgctgcc gacaggaatg gagatccggc cctgcccagg ctggatgtgg
     8821 aggaccggcc ccgcttctca tggcgcggcc tgatgatgga cagttgccgc cacatgatgc
     8881 ccgtgcggga catcaaaaaa atcctggact tgatggaaag gtacaaattc aacaccctgc
     8941 actggcacct gacggacgac cagggatgga ggctcccggt ctccaagtac cccaggctga
     9001 caaccgtggg aggcactcgg gcgcaatccc ccgtcatcgg caaccgcaac aaggcagacg
     9061 gcacgcccta ctccggccat tacaccgcgg atgaaatccg ggatgtggtg cggtatgcca
     9121 aaaaccgggg cattaccgtc attccggaag tggaaatgcc gggccacgcc tccgccgcca
     9181 tcgccgcgta tccggaactg ggcaacacgg acattccggg ctatgcgccc aaggtgcagg
     9241 aaacctgggg agtgcttccc tacaccttcg ctcccacgga aaaaaccttc ctttttctgg
     9301 aagacgtcat tgacgaagtg tgcgccctgt tcccggacag tccctatatc cacatcggcg
     9361 gggatgaggc tccaaaaaac cagtggaagc agtcccccgc agcgcagcag gtcatgaagg
     9421 acaacggcct ggccaacgag catgaactcc agagctactt catccgccgc gtggaaaaaa
     9481 taatcaacag ccgcggaaaa aggctcatcg gctgggatga aatccaggaa gggggcctct
     9541 cccccactgc caccatgatg gtctggcgca gctggatgcc gaacagcgcg cggcacgccc
     9601 tggaacaggg caacgacgtg gtgatgacgc ccaacagcca cttgtacttt gactatgacc
     9661 aggggccggg aaaacccgct gcccccgaat acgagacgat taacaacaat catctgacct
     9721 ggcagcatgt ttacggactg gaaccggttc ctcaggggac gccccgggaa cgggaaaagc
     9781 aggtgctggg ctgccaggcg aacatctgga cggaatacat tccgaacctg ccgaaatggg
     9841 aataccaagt cttcccccgt gccctggcgc tggcggaagt tgcctggacc ccgcaggagc
     9901 taaaaaatga gaaag
//
LOCUS       DAOMSU010000059         9702 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_455_length_9702_cov_2.429564, whole genome shotgun sequence.
ACCESSION   DAOMSU010000059 DAOMSU010000000
VERSION     DAOMSU010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9702
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_455_length_9702_cov_2.429564"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="PK077_04035"
     CDS             <1..603
                     /locus_tag="PK077_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRN23498.1"
                     /translation="HMNVDVQELGCDLYAFSGHKLYAPTGIGALWGKRELLEKLPPWM
                     GGGEMIKEVTFEKTVYNDIPFKYEAGTPNIGGAVGLAAAIRYVSGLGLDNIAAHEQKL
                     TDMAVEGLKAMPRLTVLAPDVPHSAVVSVLAKGIHHYDLGTLLDQMGIAVRTGHHCCQ
                     PLMCALGTTGTTRASFALYNTKEEVQTFLKSMNRALDMLS"
     gene            641..2374
                     /locus_tag="PK077_04040"
     CDS             641..2374
                     /locus_tag="PK077_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23499.1"
                     /translation="MKTVSILLLGLAIGLSGCSLHQRQEMEYAHYQDDAAAIREAHAA
                     WLILSSPSRSREWPEARKKYNACIRELAAHLKEAKRKGGMDEEKRLSLPFVIEKSPYA
                     KDSNPWFYEAIFMSDEVDPTFRLRESVTVEGMGIPLAGLAPRGGSRPHANVLKDNGNV
                     HTLTAILDFDRMVDGKPTLRTIPRLMNEHIFIGKNKVRQPLAANFSVPIALFWKLSDA
                     DGTELLGAFRPKKAINTMGLYFSEPYDPRKIPVVFTHGLMSGPATFANLTNRLLVDPV
                     IRENYQFWFFGYPSGLAWTIPASRQRQALKELMQEYNPRGTSREMNNIVMVGHSMGGL
                     ITRFNNSTKPWTLMKGLFELPPETFAGMTMGNWKNGLAPLNCDEHTLEQLQNNFIFSP
                     SSSVTRIVYMATPHRGSTFADNWIGRLGQHLIDLPSDMLEEVTRIATLSRGMFLLNPL
                     QLKDELTSIRQLSPNSSLVKYMSELRGSPNVPVHSIIGDRGRNDTPNSSDGVVKYHSS
                     HLDWSASEKIVPSGHSVQDDPASAVELRRILREHLVKVKGRKTLEEADAKAATPVWQA
                     NPSPPIILKRP"
     gene            2849..>4432
                     /locus_tag="PK077_04045"
     CDS             2849..>4432
                     /locus_tag="PK077_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HRN23500.1"
                     /translation="MSADDSFPMDQISSLFPQLENIQLVGAKGPQQIFTATLKSNFAP
                     VMLRVVPTEEAGIFGWDPEFIVRSLTIVEQSHQGLLRVYEVGQAEPFTFIISEHPPYP
                     RMADMETLPQISPQAALNLVRNMAEGLLTLHRKNIFHGGITPKLICLREDGGDALLLP
                     INIYPAQPPVDMGDFASPEWVTGAETTFTPGMDIYALGLALYILLTRKTPMEAGFAMP
                     SSLIKCSDAVDSAVSRAINPDTRERYRDLGDFITDLDKAIANPSGRNAASIPPPSSSP
                     AIPTLNMQKGQSNIYYYLIPALIVGIVLTYTCILYKKDVVRMRNDYNEQVQKENNAKA
                     EAARQANRDARRHAPATAQNAAAAIPSPVPAARPAQAPAVPKVSAEPGKINWSLQPGV
                     KVRQSSNRNMLGAYGPEKAVDGNTSSRISDVSISATGVVEGKTAWFGIDFGRETNRTI
                     EKVVIYTPANLTLLGTMEKFKVILYGNDKNVLAEKTFSTTPSEKSTNVTSWKLDAPVK
                     ARALRVESANPSQPLALTEV"
     assembly_gap    4433..4497
                     /estimated_length=65
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4922..5269)
                     /gene="rplS"
                     /locus_tag="PK077_04050"
     CDS             complement(4922..5269)
                     /gene="rplS"
                     /locus_tag="PK077_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRN23501.1"
                     /translation="MNIINKIEQEQLKADVTPFNVGDTIKVHTRVIEGGKERIQIFQG
                     IVIAKRGSGINEAFTVRKISYGEGVERVFPLHTPRIAKIEVVNRGKVRRAKLHYLRGR
                     IGKDAMIVKSANR"
     gene            complement(5379..6917)
                     /locus_tag="PK077_04055"
     CDS             complement(5379..6917)
                     /locus_tag="PK077_04055"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HRN23502.1"
                     /translation="MFKSGLAVLFLLASWFSASCAYELLPAHVAVVYNGKSELSRRMA
                     REYARVRGVPEGNLVSLDCPMTGEISRKEYEETIRVPLLETARKQRWWVPSGIASSPL
                     MNRKIFVLVLMADLPMKIRHETPAPLPGKGVNQMQTDRAAVDSELALLAVGGYERKSW
                     QGNPYFNKREDFVGSGLPSFLVCRLDGLTPDTCMRLVAEPANVEKKGLWGWAVVDRGG
                     PYAQGDRWLDDVFERVREAGIPAYLDDWPQTLPEKFPLSRDTALYCGWYAGNANGPFS
                     DPSFRFRPGAIAMHLHSFSAADFKVPGRGWSSALLEKGAAVTVGNVYEPFLGACHRFD
                     IFVDRLLDGYTVAEASWMSMPVLSWQGVVFGDPLYRPYARMKDMDVEPTEEDRYFQGW
                     WASSVQFGDRWKDRSARLMESARKAPFSCLYEALALECLYRKEPARAGELLSSALDGA
                     ADARTRARLLLEILMAERARGGNKAFLQRADGIRGLMSSSAFLPALEEWLARVAPPPA
                     PPKK"
     gene            7084..7506
                     /gene="mce"
                     /locus_tag="PK077_04060"
     CDS             7084..7506
                     /gene="mce"
                     /locus_tag="PK077_04060"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRN23503.1"
                     /translation="MIQQIDHIGIAVKSLDATVPYYRDALGLGEPHIEEVPTQKVRVA
                     MFDVAGVHIELLEPTSPESAIAKAIEKKGEGIHHIAFKTNDIAGNISQAKEAGLTVLN
                     DPPVPGAHNTQVTFLHPKCTFGVLTEFCQHPGGCECGK"
     gene            7538..9103
                     /locus_tag="PK077_04065"
     CDS             7538..9103
                     /locus_tag="PK077_04065"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRN23504.1"
                     /translation="MAIDPKLIDDLNSRRKKVILSGGQEKIDKRHEKGEMTARDRMGY
                     LFEEGTFSEIGMHVRHNCHNFGMGKKEIPGDGVVSGFGLVDGRPVACAASDFLAQGGS
                     LGYMHAMKIADAQKYALKAGIPMVTVNDSGGARLQEGVAALSGYAQVFYNNVLASGVV
                     PQISMILGPCAGGAAYSPALTDFIIMRNSGNAGMYITGPKVIEQVTYEKCTMDDIGSA
                     AIHATVSGNVHFVADSDAHAMDILKRLLSFLPSNNTEEPPHKLDTPLDLSADEGMSDL
                     IPGDNRTPLDVQPIISRLVDNGDFLEVHKDFAKNVVVGFGRICGVVVGIIANQPNVKA
                     GCLDIDSSDKAARFIRFCNAFNIPLVNLVDVPGFLPGKNQERGGIIRHGAKLIFAYSQ
                     ATVPKVTLIMRKAYGGAYIAMCCKDLGADAVFAWPGAEIAVMGAEGAVPVLYGRELKA
                     VEDPAEKAKRQGELLEEYREAFYNPYVAAGMGQITEVINPEETRAKIAFALRTLLNKK
                     EVRPAKKHGNIPL"
     gene            9124..9570
                     /locus_tag="PK077_04070"
     CDS             9124..9570
                     /locus_tag="PK077_04070"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HRN23505.1"
                     /translation="MLLTTLAFAQGIANVMNNLNYQIVGIMVVMMCLGGLAVILTISG
                     SIAAAIQNRAKAKTAAPVAAPAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVA
                     VISAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
BASE COUNT         2149 a         3124 c         2523 g         1841 t
ORIGIN      
        1 cacatgaatg tggatgttca ggaactgggc tgcgacctgt atgccttttc cggccacaag
       61 ctgtacgcgc ccaccggcat cggcgcgctg tggggaaaaa gggagctgct ggaaaaactg
      121 ccgccgtgga tgggcggcgg ggagatgatc aaggaagtca cctttgaaaa aaccgtttac
      181 aacgacattc ctttcaaata tgaagcggga acgcccaaca taggcggggc tgtgggtctg
      241 gcggccgcca tccgctacgt ctccggactg ggcttggaca acatcgccgc ccatgaacag
      301 aaactgacgg atatggcggt ggaaggcctg aaagccatgc cgcgcctgac cgtactggcg
      361 ccggacgtgc cgcacagcgc cgtggtctcc gttctggcga agggcatcca ccactatgac
      421 ctgggtacgc tgctggatca gatggggatt gccgtaagaa ccgggcacca ttgctgccag
      481 ccgctcatgt gcgccctggg caccaccggg acaacccgcg cctcctttgc cctgtacaat
      541 acgaaagagg aagtgcagac cttcctcaaa tccatgaacc gggcgctgga catgctctcc
      601 tgaatcaccc tcccttccct gcttattgac accgcgcatc atgaaaacgg tttccatcct
      661 tctcttagga ctggccatag gcctttccgg ctgttccctg caccagaggc aggaaatgga
      721 gtacgcccat taccaggatg acgccgcggc tatccgggag gcccatgccg catggctcat
      781 tctttcctct cccagccgca gccgggaatg gccggaagcc cggaaaaaat acaatgcgtg
      841 cattcgggag ctggccgccc acctgaagga agccaaacgc aaaggcggca tggatgagga
      901 gaagcgcctg agccttccct tcgtcattga aaaatctccc tatgccaaag acagcaatcc
      961 gtggttctat gaggccattt tcatgtccga tgaagtggac cccaccttcc gcctgcgcga
     1021 aagcgtgacg gtggagggga tgggcattcc tctggcgggg ctggcgcccc ggggcggttc
     1081 ccgcccccat gccaacgtgc ttaaggacaa cggcaacgtg cacacgctga cggccattct
     1141 ggactttgac cgcatggtgg acgggaaacc taccctccgc accattccgc gcctgatgaa
     1201 tgagcacatc ttcattggca aaaacaaggt gcggcagcct ctggcggcca atttttccgt
     1261 ccccatcgct ctcttctgga agctttccga cgcagacgga acggaactgc tgggggcatt
     1321 ccgccccaaa aaggccatca atacgatggg gctttatttt tccgaaccct atgacccccg
     1381 gaaaataccc gtggttttca cccacggcct gatgtccggc cccgccacct tcgctaacct
     1441 gaccaaccgt ctcctagtgg atccggtcat ccgggaaaac taccaattct ggttcttcgg
     1501 ctatccgtcc gggctggcct ggaccattcc ggccagcagg cagcggcagg ccctgaagga
     1561 gctcatgcag gaatacaatc cccgcggaac atccagggaa atgaacaaca tcgtcatggt
     1621 ggggcactcc atgggcggcc tcatcacccg cttcaataac tccaccaaac cctggacgct
     1681 gatgaaggga ctctttgaac ttcctccgga aaccttcgca ggcatgacga tgggaaactg
     1741 gaaaaacgga ctggcgcccc tgaactgtga cgaacacacg cttgaacagc tccagaacaa
     1801 tttcatcttc tccccgtcca gcagcgtcac gcgcatcgtg tacatggcca ccccgcaccg
     1861 cggttccact ttcgcggaca actggatagg aaggctggga cagcacctga tcgacctccc
     1921 ctcggacatg ctggaagaag tcacccgcat cgccactctc agccgcggca tgttcctcct
     1981 caaccccctg caactgaaag acgagctgac cagcatccgc cagctttccc cgaattcatc
     2041 cctggtcaaa tacatgtcgg agctgcgcgg ctccccaaat gttcccgttc attccatcat
     2101 tggagacagg ggcaggaacg acacgcccaa ttcctccgac ggagtggtca agtaccactc
     2161 atcccacctg gactggagcg ccagtgaaaa aatcgttccg tccgggcaca gcgtgcagga
     2221 tgacccggcc tccgccgtgg aactgcggcg catcctccgg gaacacctgg tcaaggtcaa
     2281 aggccgtaaa acgctcgaag aagcggacgc gaaagccgcc actcccgtat ggcaggccaa
     2341 tccctcgccg cctatcatcc tgaaaaggcc ctgatgcagg ggactgtttc tccttgtcca
     2401 tgcaaggcct ccttcccttc cccccttctg ctgttcctgc tgataacagc agccatcttc
     2461 cagtcaaacg gccacgcctc ggcgatcctg tttccgggtt cctgtatgtc ccggctttaa
     2521 cggttggaac gccctcgttc atccaccctc cctgccgctc ccggaaaaaa acaggaaccg
     2581 aacaggattt accttgccgc gggcatctgc ggcaacatgc cgccgcggcg gttgaaacaa
     2641 ctgaaatttt ccccgctcat tacggcctgt tcctggctgc cctgatttga ctggccggtt
     2701 acggaaaagg gcgcgctcca tccgggcatg attggcgtcc gttcagcatc agggcgtccc
     2761 gccatcaaaa tatgcccgct tatagagttg cttccgtagg ggaatttaag gtagagtggg
     2821 aatcaatact attccacctc ccttttccat gtctgccgac gattcctttc ccatggacca
     2881 gatttcctct ctctttcccc aattggaaaa tattcagctt gtcggagcca aaggccccca
     2941 gcaaattttc acagctaccc tgaaatccaa ctttgctccc gtcatgctgc gggtggttcc
     3001 cacggaagaa gccggcatct tcggctggga tcctgaattc atcgtccgct ccctcaccat
     3061 cgtggaacaa tcccaccagg ggctcctgcg cgtttatgaa gtaggacagg cggagccgtt
     3121 tactttcatc atctccgaac acccccccta tccgcggatg gcggatatgg aaacccttcc
     3181 ccaaatatct ccccaggcgg cgctcaacct ggtgcgcaac atggcggaag gactgctgac
     3241 gctgcaccgg aaaaacattt ttcacggggg catcacgccc aagctgattt gcctgcgcga
     3301 agacggcgga gacgccctgc tgctgcccat caacatttat cccgcccaac ctccggtgga
     3361 catgggggac ttcgcctctc cggagtgggt caccggagcg gaaaccacgt tcacgccggg
     3421 catggatatt tacgcactgg ggctggcgct ctacattctg cttacccgca aaacgccgat
     3481 ggaagcgggc ttcgccatgc cctcctcact aatcaaatgc agcgatgccg tggattcggc
     3541 agtctcccgc gccatcaatc cggatacccg ggaacgctac cgggacctgg gggatttcat
     3601 cacggatctg gataaagcca ttgccaatcc ttcgggaaga aatgcggcct ccatccctcc
     3661 accgtcatct tccccggcca ttccgaccct gaacatgcaa aaagggcagt ccaacatcta
     3721 ttactatctc attccggccc tgatcgtggg cattgtgctg acctatacct gcatcctgta
     3781 taagaaggat gtcgttagaa tgcgcaatga ctataatgaa caggtgcaga aggaaaataa
     3841 cgcgaaagcg gaagcggcaa gacaagcgaa cagggacgcg cgccgccacg ctccggccac
     3901 cgcgcaaaac gccgctgcgg ccattccttc acctgttccg gccgcgcgtc cggcgcaggc
     3961 gccggcagtt cccaaagttt ccgcggaacc cggcaaaatc aattggagcc ttcagcccgg
     4021 cgtcaaggtt cgccagagtt ccaaccgcaa catgctggga gcttacggtc cggaaaaggc
     4081 cgttgacggc aataccagct ccaggatttc cgacgtttca atcagcgcca caggcgtggt
     4141 tgaagggaaa accgcctggt tcggcattga cttcggcagg gagaccaacc gcaccataga
     4201 aaaggtggtc atttacacgc cggccaacct gacgctgctg ggcaccatgg aaaaattcaa
     4261 ggtaatcctc tacggcaatg acaaaaacgt gctggcggaa aaaaccttca gcaccactcc
     4321 ttcggaaaaa tccaccaacg tcacttcctg gaaactggac gctccagtca aggcgcgcgc
     4381 cttgcgcgtg gaatccgcaa atccctccca gcccctggcg ttgacggaag tgnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat
     4501 tatccgcgca gccctgcacc cgctcgcccc ccctccgggg gccatgctga aaccccgttt
     4561 cctttccccc tcccggaaat caggttgggg cagggcctga tggaatgtca cgcgctaagt
     4621 tccgggtttg gattcgcgta tgaaattttc cggtttctgc tccattctcc ggaagccatc
     4681 caggcccgga cggaggaaaa tccgaaaaac gtatttgctc cggaccggaa acagccgccc
     4741 ctctccacct tccccacccc ggaaaaacgc tctcatcccc atatggctcc cggagaaata
     4801 tcttgaagtg gctgttgttc ctgtggacct ctttcccatt caggaaaaac aatactccgg
     4861 aaataaaaaa cccggcgccg cctcacggcg ggccgggcca gcaaaatagc aattcttccg
     4921 attaccggtt ggcagacttc acaatcatgg catccttgcc gatgcggccg cgcagatagt
     4981 gaagtttggc gcggcggact ttgccgcggt tcacgacttc aatcttggca atgcggggag
     5041 tatgcagggg gaatacgcgt tccacgcctt caccgtagga aattttgcgg acggtgaaag
     5101 cttcattgat gccggaaccg cgcttggcga tcacaatgcc ctggaaaatc tggatacgtt
     5161 ccttgccgcc ttcaataaca cgagtgtgaa ccttgatggt atcacctacg ttaaatgggg
     5221 tcacatccgc cttgagttgc tcttgctcga ttttgttgat aatgttcatc ttgtgttatc
     5281 tattgctggt gaatatcagt aaaaggaaaa cttacgggac gcagaatatg agccataatc
     5341 ccctaaaagg caatccaaat ttgccgtacg tccttaattt attttttcgg cggggccggc
     5401 ggcggagcca ccctggcaag ccattcctcc agcgccggaa gaaaagcgga ggaagacatg
     5461 aggccccgga tgccgtccgc acgctgcaga aaggccttgt taccgccccg ggcccgttcc
     5521 gccatcagga tttccagcag cagacgcgcg cgggtgcggg cgtcagccgc gccgtccaga
     5581 gcggaggaaa ggagctctcc ggcgcgggcc ggttccttcc ggtacaggca ttccagcgcc
     5641 agagcttcat aaaggcagga aaaaggggcc ttccgggcgg actccatcaa acgcgcggaa
     5701 cggtccttcc agcggtctcc gaactgcacg gaggaagccc accacccctg gaaataacgg
     5761 tcttcttccg tcggctccac gtccatatcc ttcatgcggg cgtatggacg gtacaggggg
     5821 tctccgaaca ccaccccctg ccaggacagc acaggcatgc tcatccagga agcctccgcc
     5881 acggtatagc catccaggag gcggtccaca aaaatatcaa agcggtggca ggcgcccagg
     5941 aacggctcat acacgtttcc caccgtcaca gcagccccct tctccagcag ggcggaactc
     6001 catcccctgc cggggacctt gaaatcggcg gcgctgaagg aatgcagatg catggctatc
     6061 gccccgggcc ggaagcggaa cgaaggatcc gaaaacggtc cgttcgcatt ccccgcatac
     6121 cacccgcagt aaagagccgt atcccggctc aaggggaatt tttccggcag ggtctggggc
     6181 cagtcatcca gataagcggg aatgcccgcc tcccttacgc gctcgaacac gtcgtccagc
     6241 caccggtccc cctgggcata ggggccgccc cggtccacaa cggcccaccc ccataaacct
     6301 tttttctcca cgttcgccgg ttccgccacc agccgcatgc aggtatcggg cgtcagtccg
     6361 tccaggcggc ataccagaaa cgaaggaagg ccggaaccca caaaatcctc ccttttatta
     6421 aaatagggat tcccctgcca ggacttcctt tcataaccgc ccaccgccag cagagccagt
     6481 tcggaatcca ccgcagcgcg atccgtctgc atctggttga cgcctttccc gggaagggga
     6541 gcgggcgttt catgccgtat cttcatcggc aggtccgcca tcagcaccag cacaaaaatc
     6601 ttccggttca tcaaggggga ggacgcaatg ccggaaggca cccaccaccg ttgtttccgc
     6661 gccgtctcca gcagcgggac gcggatcgtc tcctcatact ccttccgcga aatctcgccg
     6721 gtcatggggc aatccaggga aacgagattc ccttccggaa cgccgcgcac ccgcgcatac
     6781 tccctggcca tgcggcggct cagctcggat tttccgttat aaaccacggc cacatgagcg
     6841 ggcagcagct cataggcaca cgaggcggaa aaccacgacg ccagcagaaa taacacggct
     6901 aatccactct tgaacatgcc cctaccatac ctcatcttcc ggggcaatca agcaggtgaa
     6961 aggggcggcc acgaattcgc catttttccg cttttcgctt cacatcctaa aaaaatacgc
     7021 tttaataact cttgatgtat tgcaggcctc ccccctgcat tcccctataa ccatcacacg
     7081 catatgattc agcaaattga ccatatcggc atcgccgtca agagtcttga cgccaccgtt
     7141 ccctattacc gcgatgcgct cgggctcggc gagccccaca ttgaggaagt tcccactcaa
     7201 aaggtccgcg tcgccatgtt tgacgttgcc ggcgtccaca tcgaactgct cgaacccacc
     7261 tctccggaaa gcgctatcgc caaggctatt gagaaaaaag gggaaggcat ccaccacatc
     7321 gctttcaaga ccaatgacat cgccggaaat atctcccagg ccaaggaagc gggccttacc
     7381 gtgctgaacg acccgcccgt ccccggcgct cacaacacgc aggtcacctt cctgcatccc
     7441 aagtgcacct tcggcgtcct gaccgaattc tgccagcacc ccggcggctg cgaatgcggc
     7501 aagtaacatt ctccctattc accataccac tataccaatg gctattgatc ccaaattgat
     7561 tgacgatctg aacagccgcc gcaagaaggt aatcctcagc ggcggtcagg aaaagatcga
     7621 caagagacat gaaaagggcg aaatgacagc ccgcgaccgc atggggtacc tctttgagga
     7681 aggcaccttc tcggaaatcg gcatgcatgt gcgccacaac tgccacaact tcggcatggg
     7741 caagaaggaa atccccggcg acggcgtcgt ttccggcttc ggcctggtgg acggccgtcc
     7801 ggtggcctgc gcggcctccg acttcctggc ccagggcggg tccctcggct acatgcacgc
     7861 catgaaaatt gcggatgccc agaagtacgc cctgaaagcc ggcatcccga tggtgaccgt
     7921 gaacgactcc ggcggcgcgc gcctccagga aggcgtggcg gccctttccg gctatgccca
     7981 ggtgttctac aacaatgtgc ttgcctccgg cgtggttccg caaatctcca tgattctggg
     8041 tccctgcgcg ggcggcgcgg cctactcccc cgccctgacg gacttcatca tcatgcgcaa
     8101 ttccggcaac gccggcatgt acatcaccgg ccccaaggtg attgaacagg tcacgtatga
     8161 aaaatgcacg atggatgaca tcggctccgc cgccattcac gccaccgtat ccggcaacgt
     8221 ccatttcgta gcggacagcg acgcgcacgc catggacatc ctgaagaggc tgctttcctt
     8281 ccttccttcc aacaacacgg aagaaccgcc ccacaaactg gataccccgc ttgacctgag
     8341 cgcagacgaa ggcatgagcg acctgattcc cggcgacaac cgcacgccgc tggacgtcca
     8401 gcccatcatc agccgcctgg tggacaacgg ggacttcctg gaagtgcata aggacttcgc
     8461 caaaaatgtc gtcgtcggct tcggccgcat ctgcggcgta gtagtcggca tcatcgccaa
     8521 ccagcccaac gtgaaagccg gctgcctgga tatcgactcc tccgacaagg ccgcgcgctt
     8581 catccgcttc tgcaacgcgt tcaatattcc gttggtgaac ctggtggacg tgcccggctt
     8641 cctgcccggc aagaatcagg aacggggcgg cattatccgc cacggtgcca aactcatctt
     8701 cgcctattcc caggccacgg tgcccaaagt caccctgatc atgcggaaag cctacggcgg
     8761 cgcctacatc gccatgtgct gcaaggacct gggcgctgac gccgtcttcg cctggcccgg
     8821 cgcggaaatc gccgtcatgg gcgcggaagg cgccgttccg gtgctctacg gccgtgaatt
     8881 gaaggctgtg gaagacccgg cggaaaaagc caaacgccag ggagaactcc ttgaagaata
     8941 ccgggaagcc ttctacaacc cgtatgtggc ggccggcatg ggacagatca cggaagtcat
     9001 caatccggaa gaaacccgcg ccaaaatcgc cttcgccctg cgcaccctgc tgaacaagaa
     9061 ggaagtgcgc ccggccaaga aacacggcaa cattccgctc taacatccat ctcaacggat
     9121 catatgttac tcacaacact cgcctttgca cagggcatcg ccaacgtcat gaacaatctg
     9181 aactatcaga ttgtcggcat tatggtggtc atgatgtgcc tgggtggcct ggccgtcatt
     9241 ctgaccatca gcggctccat cgccgccgcc attcagaaca gggctaaagc caaaaccgcc
     9301 gctcccgtgg ccgcgcctgc cgccgccccc gctgcggcgg gcaaaccggg catgactccg
     9361 gaaatgctcg ccatcctctc cgcagcggtg gactccgcca tgacggagct cacgccggaa
     9421 atggtggccg tcatctccgc cgccgtggac gcgggcctgg acggcatgag ccaccgcatt
     9481 gtggaaatca agcaatcccc gggctccggc tatgcggccg caggcagggc ggaaatcttc
     9541 gcctcccacc gcattcggcc ttcccgttga actacagcat tattcaccct tacccccgcc
     9601 ccggcgggaa ccatttaact taatactcca taccatacat tatcatgaag aaacttcgca
     9661 tcaccgtaga cggcaaggta tttgacgtat ccgtagaact cc
//
LOCUS       DAOMSU010000060         9671 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_460_length_9671_cov_2.802205, whole genome shotgun sequence.
ACCESSION   DAOMSU010000060 DAOMSU010000000
VERSION     DAOMSU010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9671)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9671)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9671
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_460_length_9671_cov_2.802205"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1512
                     /locus_tag="PK077_04075"
     CDS             172..1512
                     /locus_tag="PK077_04075"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HRN23506.1"
                     /translation="MNRLDQLATLLRFPSISAQKEHARDVSDCADWLVDKLSGMGLEA
                     KACKTPLHPIVLARSPREEGKPTVLIYGHYDVQPVDPVELWESDPFEPEVRGGKIYAR
                     GATDNKGQLFAHILGVEELLRQNGGHLPVNVIFLLEGEEEVGSGSLSQFIKEHREELA
                     CDVIVVSDTGMAAPDTPTFSYGLRGLAGAEIIVKGPSADLHSGVFGGAVANPIAALAE
                     IIASFHDEEGRVAVRGFYDGVEPIATWERSMWATVPGMSNEELAQLTGVETVVTEAGF
                     TGAECIFARPTLEINGIGGGYQGEGSKTIIPSHAFAKISCRLVPGQQPERIETLLEEH
                     VKKHSPKGVTVEFRRGHGGNAYVCDPNSEFGLAAQQALEEAFGHKPVLVREGGSIPII
                     EEMKRVLGADALMLGMCLPDARIHSPNENFPVDLFSKGIDMSQILLRRLGQIKH"
     gene            1521..2399
                     /locus_tag="PK077_04080"
     CDS             1521..2399
                     /locus_tag="PK077_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN23507.1"
                     /translation="MDQPVAAEIVNLHKTFKTGLGKPVVHAVRGIDMNIRQGEVYGLI
                     GPNGSGKSTLMKALLGLVRPTEGSCSIFGRSSLAPDSKEEVGFLPENPYFYKFLTGAE
                     TVSFYGRLCGLSGKSLKAKVRELLELVGLADAADRRLGGYSKGMLQRIGMAQALVQSP
                     RLLVLDEPTAGVDPLGSRDIRNIIENLKGRGMTVFLCSHLLEQVQEVCDRVGVIFKGL
                     LIAEGSVNELTRDSDKQEILLEHASPELMERLKNMVKEDGRAVWLEDGHPRNSLESVF
                     LKSLLEWKEKHPNPES"
     gene            2366..2956
                     /locus_tag="PK077_04085"
     CDS             2366..2956
                     /locus_tag="PK077_04085"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HRN23508.1"
                     /translation="MEGKASQSGIVSGPAAAKREVRRMIRERVGGLDAAAREAMSRSI
                     CRRVSSLVREKNFRRVAVFAARPGEVDLLSLLDLLPDREWYFPLVHEGRRLSFHRVCH
                     HGELVAGSWGIREPGPDCPELHAGEMDLIVLPGAAFTRDGKRLGYGGGFYDTLLAGPA
                     AGVPLAGVCFPCQLLDDLPMEAHDRHVDMVIAPEGE"
     gene            complement(2981..4648)
                     /locus_tag="PK077_04090"
     CDS             complement(2981..4648)
                     /locus_tag="PK077_04090"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HRN23509.1"
                     /translation="MLTGGKNVIPHAVRDVADAAAAFSARIGPDRHFDSQEKVEEALA
                     ECCSPQNVTVIGMTGSGKSSTLDALAGERFCSRVSSEATLVRWQYRPHPAPHTHEWVL
                     DLFYPSDALLNLEFWDTIGLEQEDAPRKLKHIVPKSDAILVVISASDGNHSVLWDYLQ
                     TLEERLHSRMVLVITHAELLSFEKLQSLKEELRSTSRERLGVQLPLYPVTTAGEHAGE
                     GVEALTACVQEVLKRSPLTDKRVERLLLATDSLLEEQGKVLDELSRLSKMDSGFLTSI
                     DREIDRIQLQMEDEMPARLKAYAQYVQDCVPRLGKKSARQMGRFLSIRRTMILHKLPP
                     VIDEWFYELVKSGIEERHAYYNKEFLNICQTHWNHVRPRVKEQWDCDIGDFPAAKLQT
                     DLEVYKERLGRSIYMPLKDFGIKPCLSKLFLDQEDVMRTEIKLMLLLVILGALLGCFG
                     EHAAGFACVGAALAVWVGGNLGLAWMQFRLSRQIVAAAAEMHIAVECGLRTPLYEAMI
                     SGLSDYRKLYADIRGQVAGGVEYLQPLLNARYDLFYKLTVQKHRFRI"
     gene            complement(4851..5423)
                     /locus_tag="PK077_04095"
     CDS             complement(4851..5423)
                     /locus_tag="PK077_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HRN23510.1"
                     /translation="MSIRQNLEHILSRIRAAELRAGRPAGSVRLVAVSKTFPAEDVKA
                     CHDAGQRIFGENKVQEGLAKIPSLPPDTEWHLIGPLQRNKVRKALEYFTLIHAVDSLR
                     LARFMDTVAQETGKRPRILLEVNVGNEDSKFGFFPETLEKEWSELSSLNHLEIAGLMC
                     IPPPVENPEEARPYFRRLRELSCPAGEEPR"
     gene            complement(5434..5991)
                     /locus_tag="PK077_04100"
     CDS             complement(5434..5991)
                     /locus_tag="PK077_04100"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HRN23511.1"
                     /translation="MMEHEDISNENLAGHLLVAAPYLDGAGFHHSVIFLSRAEKEFVI
                     GHILNHPSGMNVGDVARHTEIPESLYAVPIFKGGPVERNQLIFAAFIRTEDKLRVQFH
                     LQEEQALEYLEDPRAILRAYVGHSGWTPPQLRRELNDRAWYVSPMVPDICLETDSSKV
                     WAMAMRRLSPLHHIMSHASAQPSLN"
     gene            complement(6106..6963)
                     /locus_tag="PK077_04105"
     CDS             complement(6106..6963)
                     /locus_tag="PK077_04105"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRN23512.1"
                     /translation="MPAPLSQAARLIQLAGAAFAVFGVLASCSRPDYEPTAIVKDGAV
                     VSVRDQKMAVMRNGRVVKTYPVSTSKFGLGDRKGSCRTPLGAHRIASKIGSGQPKGMV
                     FKSRKPTGECVAPNSPGRDPIVTRIMWLEGLEPRNRNAHSRLIYIHGTAEERTIGTPA
                     SYGCVRMKSNDVYEAFNLLRTGDPVVIERCSIDASLKALAQAEKMQDSSLLVMASAPD
                     RMENANTASVISHRRRTSRPPAVALSRKQTASTGKRSIPARRAVAHRTSGNSRTAVRS
                     SGGTRYTLG"
     gene            complement(6974..8491)
                     /locus_tag="PK077_04110"
     CDS             complement(6974..8491)
                     /locus_tag="PK077_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRN23513.1"
                     /translation="MSLNYNFIWLLCYLLVLVGLAGYGFHRLSIVYLYWKNRNNKPQP
                     KARFQELPVVTVQLPMFNEKFVVDRLLESVAALDYPQDKLEIQILDDSTDDTTEQCYR
                     KVEELKSRGFDAVCIHRTDRTGFKAGALEAATKVAKGEFLLILDADFVPEPDLLQKTI
                     HFFTDENVGLVQTRWGHINREYNLLTRIQGMYLDGHFAMEQTARNRSGRFFTFNGTAG
                     IWRKYVIGDAGGWSHDTLTEDMDLSYRVQLKGWRFIYLNDVVTPAELPVDMDGFKSQQ
                     HRWTKGSIQVCQKILLDIWRSNAPLKAKVEATTHLTCNYSYLLLALLCFLVYPICTQR
                     IPENETVFMWFVNVALFFMTSVAVCIFYMSAQIVVRPKSWWKELPYLPLLLTLSVGMA
                     VNNAKAVLEAIFGHQSAFVRTPKYGVDRKEDRRHVSQLKRNGYRAIKSVVIPVLEIAC
                     GTFFLNLIIIMIRQGHYLSPILMIPFLGFYYTGFCSLGRMISNLLPAKQTAKAKN"
     gene            8626..9516
                     /locus_tag="PK077_04115"
     CDS             8626..9516
                     /locus_tag="PK077_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23514.1"
                     /translation="MKRLYLLAAAVSVTGGMAFSAPQEDAPEKEAAVLDVQTPPSGKQ
                     DQPAIPERLLDDSHMNEELGINEFTAPSIRKIFEDLEGLPEIPEKVALRARPERPPMD
                     RCSLALQLGGMMADGFVIVQCGKMNEVKPIALDLSSYAKAIGAGERVNRHAASLLKNA
                     EDGDLSSFKNNLALIQSDVEQELASLRDSDMANLIGLGGWIRALDAATAALDNKFAED
                     KAKVIFQPDVPEYFHYILDGVEPEMKERRDIAKIMNLLTILQEQMTLAPDAKPAKEGV
                     EAIRKTTLELIKAALAPVES"
BASE COUNT         2035 a         2570 c         3041 g         2025 t
ORIGIN      
        1 tggatttgct gaacataatc ctatataaag gaacaaaaca cacgcgagag caatcaaata
       61 ttgttcccgg cgccatgtgc gggctccatt ttttgaaaaa aggcccgctc cggcatggaa
      121 tgccacactt taccggttga tttgaagccc cagacatggt ctgatgaagg catgaacaga
      181 ctggatcaat tagccacctt gttgagattt ccaagcattt ccgcacagaa ggaacacgcc
      241 cgggacgtga gtgattgcgc tgactggctg gtagacaaat tgtccggcat gggattggaa
      301 gccaaggcct gtaaaacgcc cctccatccc atcgtgctgg cccgcagccc gcgggaggaa
      361 ggaaagccta ccgtgctgat ttacggccat tacgacgtgc agcccgtaga tcctgtggaa
      421 ttatgggaat ccgacccgtt tgaaccggaa gtgaggggcg ggaagattta cgcccgcggc
      481 gctacggaca acaaggggca gctgttcgcc catattctgg gcgtggagga attgctccgc
      541 cagaacggag gccaccttcc cgtaaatgtg attttccttc tggaagggga ggaagaagtg
      601 ggcagcggca gtctttccca gtttatcaag gagcacaggg aagagctggc ctgcgacgtt
      661 attgtggttt ccgataccgg catggccgct ccggacaccc ctacgttttc ctacgggctg
      721 cgcggcctgg ccggggcgga aattatcgtc aaaggccctt ccgcggatct tcattccggc
      781 gtgttcggag gagcggtggc caatcccatc gccgctcttg cggaaataat cgcctccttc
      841 catgatgaag aggggcgtgt ggccgtgaga gggttttacg acggcgtgga acctatcgcc
      901 acatgggaac gcagcatgtg ggccacggtg cccggcatga gcaatgagga actggctcag
      961 ctgacgggcg tggaaaccgt ggtgacggag gccggtttca ccggagcgga atgtattttt
     1021 gcccgcccga cgctggaaat caacggtata ggaggaggtt accagggtga gggaagcaag
     1081 acgattatcc ccagccatgc ctttgccaaa atttcatgcc gcctggtgcc gggacagcag
     1141 cctgaaagga ttgaaaccct gctggaagaa catgtaaaga aacattcccc caagggcgtc
     1201 acggtggaat tcaggcgggg ccatggcggg aatgcttacg tgtgcgatcc gaattccgag
     1261 ttcggtcttg ccgcccagca ggcgctggaa gaggcttttg gccacaaacc cgtgctggta
     1321 agggaaggag gcagcatccc gatcattgag gaaatgaagc gcgtgctggg agcggatgcc
     1381 ctgatgctgg gcatgtgcct tccggatgcc cgcatccatt cccccaatga aaactttccc
     1441 gtggatttgt tctccaaggg cattgacatg agccagattc ttctgcgccg cctggggcag
     1501 atcaagcatt gacctttttt atggaccagc ctgtagccgc tgaaatagtc aacctgcaca
     1561 agacctttaa aacaggtctt ggcaaacccg tggtgcatgc cgtgcggggg atagacatga
     1621 atatccggca aggggaggta tatggcctca ttggcccgaa cggctccggc aagtccaccc
     1681 tgatgaaggc cctgctgggg ctggtgaggc ctacggaagg ttcctgttcc attttcggca
     1741 ggtccagcct ggctccggac agcaaggagg aagtgggctt tcttccggaa aacccctact
     1801 tttacaaatt cctgacgggg gcggaaactg tttcctttta cggcaggctg tgcggcctga
     1861 gcggaaaatc cctgaaagcg aaagtcaggg aactgctgga gctggtggga ttggctgatg
     1921 cggcggacag gcggctgggc ggctattcca aggggatgct ccagcgcatc ggcatggcgc
     1981 aggccctggt tcagtccccc cgcctgctgg ttctggacga accgaccgcc ggcgtggatc
     2041 ctctggggtc gagggatatc cggaacatta tcgaaaacct gaaagggcgt gggatgaccg
     2101 tattcctgtg ttcccacctg ctggaacagg tgcaggaggt ttgcgaccgg gtgggcgtca
     2161 tttttaaagg gctgctcatt gcggaagggt ctgtgaatga gctgacgcgg gattccgaca
     2221 agcaggaaat acttctggag catgcttccc cggaattgat ggaacggctg aagaatatgg
     2281 tgaaggaaga cggccgtgcc gtttggctgg aggatgggca tccgcgcaac tccctggaaa
     2341 gcgtatttct aaaaagtttg ttggaatgga aggaaaagca tcccaatccg gaatcgtaag
     2401 cggtccggcg gctgccaaac gggaagtgcg ccgcatgata cgtgaaaggg tgggcggatt
     2461 ggatgccgcc gcccgggagg ccatgtcccg ttccatctgc cgccgtgtgt cgtctctggt
     2521 gcgggagaag aatttccggc gcgtggctgt ttttgccgcc aggccgggag aggtggattt
     2581 gctttccctg ctggatttgc ttccggaccg ggaatggtac ttcccgctgg tgcatgaggg
     2641 gagaaggctg agttttcacc gtgtatgcca tcacggggaa ttggtggcgg gcagctgggg
     2701 catacgcgaa cccggtccgg actgcccgga actgcatgcg ggggaaatgg atttgatagt
     2761 gctgcctggg gccgcattta ccagggacgg caagcggctg gggtatggcg gcggatttta
     2821 tgatacgctg ctggccggcc cggctgctgg ggtgccgctg gcgggagtct gcttcccctg
     2881 ccagcttctg gacgacctgc ccatggaagc ccatgacagg catgtggaca tggtgattgc
     2941 gccagaaggg gagtgacctc tccggagaaa gggaaagagg ctagatacgg aaccggtgtt
     3001 tctgcacggt cagtttataa aacaggtcgt agcgggcgtt gagcaggggc tggaggtatt
     3061 ccacccctcc ggctacctgg ccgcggatgt ccgcatacag tttccggtag tcggaaagtc
     3121 cgctgatcat ggcttcatac aggggggtgc gcaacccgca ttccacggca atgtgcattt
     3181 ccgccgctgc ggccacaatc tggcgggaca ggcggaactg catccaggcc agccccaggt
     3241 tgccgcccac ccagacggcc agggccgcgc ccacgcaggc gaaccctgct gcgtgctctc
     3301 cgaaacagcc cagcagggct cccagaatca ccagaaggag catcagcttg atttccgtcc
     3361 tcatcacatc ctcctggtcc agaaacagct tgctgaggca cggcttgatg ccgaagtcct
     3421 tcaggggcat gtaaatggaa cgccccagcc tctctttgta cacttccaga tccgtctgga
     3481 gctttgcggc cggaaagtcc cctatgtcgc agtcccactg ttccttcacg cgtgggcgca
     3541 catggttcca gtgcgtctgg cagatgttca ggaattcctt gttgtaatac gcgtgccgtt
     3601 cctcaatgcc ggatttgacc agctcataaa accattcgtc aatgacggga ggaagcttgt
     3661 gcaggatcat cgtgcgccgg atggatagga aacgccccat ctggcgtgcc gactttttcc
     3721 caagacgcgg cacgcagtcc tgcacgtatt gcgcgtatgc cttgaggcgt gccggcattt
     3781 catcctccat ctggagctgg atgcggtcga tttcccggtc aatagacgtc aggaagccgg
     3841 aatccatctt ggaaaggcgg ctcagttcat caagcacctt cccctgttct tccagaaggc
     3901 tgtccgtagc cagcagaagg cgttccacgc gcttgtccgt cagcgggctg cgtttcagca
     3961 cttcctgaac gcacgccgtc agggcttcca ctccttcccc tgcatgctct ccggcggtcg
     4021 tgacagggta caggggcagc tgcactccca ggcgttcccg ggaggtggag cgaagctcct
     4081 ctttcaagga ttgaagcttc tcaaaggaga gcagctccgc atgggtgatg acgagaacca
     4141 tgcgggaatg gagacgttcc tccagcgtct ggagatagtc ccacaggacg gaatggttgc
     4201 cgtcactggc ggaaataacc acaaggatgg catccgattt gggaacgatg tgcttcagct
     4261 ttctgggggc atcctcctgt tccaggccga tggtatccca gaactccagg ttgagcaggg
     4321 cgtccgacgg gtaaaaaagg tccagaaccc attcatgggt gtgtggggcg ggatgaggcc
     4381 tgtactgcca gcggaccagt gtggcttcac tggacacgcg ggaacagaac cgttccccgg
     4441 ccagggcgtc cagcgtagag gatttgccgg accctgtcat gccgatgacg gtgacattct
     4501 gcggagagca gcattcggcc agggcttcct ccaccttttc ctgggaatcg aaatgacggt
     4561 cggggccgat gcgggcggaa aacgccgcgg cggcatccgc tacgtcccgc accgcatgcg
     4621 gaatcacgtt ttttcctcct gtcagcatgg ggtgatgaaa tcaggtctcc gtccgggctg
     4681 ttccggtttt ccagaacaaa cctacacgcc gcttttcctt ctgtccaact aaaatcacga
     4741 agcgggagcg tacgagcggc cgccgaaaat ggccgtccct acgcggacga gcgtggctcc
     4801 ttcctctatt gccgtttcaa agtcgtggct catgcccatg gacagttccg tcagcgtggc
     4861 tcctcccctg ccggacagga cagctcccgg aggcggcgga aatagggccg cgcctcctcc
     4921 ggattttcca ccgggggggg aatgcacata aggccggcga tttcaagatg gttcagggag
     4981 gacagctccg accattcctt ttccagggtt tccgggaaga atccgaactt gctgtcttca
     5041 ttgcctacgt tcacttccag caaaatgcgg ggccgttttc ccgtttcctg agccacggta
     5101 tccatgaacc gggccaggcg cagggagtcc acggcatgga tcagcgtgaa atattccagg
     5161 gccttgcgca ccttgttgcg ctggaggggg ccaatgagat gccattccgt atccggagga
     5221 agggaaggga tttttgccag tccctcctgg accttgtttt caccgaaaat gcgctggccc
     5281 gcatcatggc acgctttcac atcctctgcg ggaaaagttt tgctgacggc gacgaggcgg
     5341 acggaacccg ccggacgtcc ggcgcggagt tccgctgcgc gtatgcggga gagaatatgt
     5401 tccagatttt gtcggatgct catgatggat ggttcagttc agggatggct gtgcggacgc
     5461 gtggctcatg atatggtgca ggggagagag ccgccgcatg gccatggccc agaccttgga
     5521 agaatccgtt tccaggcaga tgtccggcac catgggggaa acataccagg cacggtcatt
     5581 cagttcccgg cgcagctggg gcggcgtcca tccgctatgg cctacgtagg cgcgcaggat
     5641 ggcgcgggga tcttccaggt attccagggc ctgttcctct tgcagatgaa actggacgcg
     5701 caatttatcc tccgtgcgga tgaaagcggc gaagatgagc tggttgcgtt ctacgggccc
     5761 tcccttgaag atgggcacgg cgtaaaggga ttccggtatt tccgtatggc gggctacgtc
     5821 ccccacattc atgccggacg gatgattcaa aatatggccg atgacaaatt ccttctccgc
     5881 ccggctcagg aagatgacgg aatgatggaa tccggcgcca tccaggtagg gggcggcgac
     5941 cagaaggtgc cccgccaggt tttcattgct gatatcttcg tgttccatca tggccataaa
     6001 tctacgttac caaatgtgac ggatgcaggc aaccatgcgt tcaaacggcc atgtcaggag
     6061 gcggcgaaaa cggaactccg gaaaaacagg agggaagggg agcttttacc cgagtgtata
     6121 gcgggtccct ccggaggaac ggacggcggt tctggaattt ccggacgtcc tgtgggcgac
     6181 cgccctcctg gcgggaatgg atctcttccc ggttgaggcc gtttgcttgc gggaaagggc
     6241 aacagcgggg gggcgggaag tcctgcggcg gtgggaaatg acggaggccg tattggcgtt
     6301 ctccatgcgg tctggagcgg aagccatgac cagcagggaa gaatcctgca ttttttccgc
     6361 ctgggccagg gctttcaggg aggcgtcaat ggaacagcgt tcgattacga ccgggtctcc
     6421 ggtacggagc aggttgaagg cctcatatac gtcattgctc ttcatccgga cgcagccgta
     6481 ggaggcgggg gtgccgatgg tgcgttcctc cgccgtaccg tggatataaa tcaggcgcga
     6541 gtgagcgttc cggttacggg gttccagacc ttccagccac atgatgcgcg tgacgatggg
     6601 gtcccgcccc ggagaattgg gcgcgacgca ttccccggtg ggcttgcggc ttttaaacac
     6661 catgcccttg ggctgtccgc tgccgatttt ggaagcgatg cgatgggcgc ccagcggagt
     6721 gcggcagctt ccctttctgt ctcccaggcc gaatttggaa gtgctgacag ggtaggtttt
     6781 caccacccgg ccattgcgca tgacggccat tttctggtcc ctgacgctga cgaccgcgcc
     6841 gtctttcacg atggcggtgg gctcataatc cggacgggaa caggatgcca ggactccgaa
     6901 cacagcgaaa gctgcgccgg cgagttgaat caggcgtgct gcttgcgaga gaggcgcagg
     6961 catgggaaag gcgtcagttc ttggctttgg cagtttgctt ggcgggcagc aggttggaaa
     7021 tcatgcggcc cagggaacag aatcccgtgt aatagaaacc caggaaagga atcatcagga
     7081 tgggggaaag gtaatggccc tggcggatca tgatgatgat caggttcagg aagaaagttc
     7141 cgcaggcgat ttccagaaca ggaatgacga cggatttaat agcccggtac ccgttgcgtt
     7201 tgagctggga gacgtgccgg cggtcttcct tccggtccac gccgtatttg ggcgtgcgga
     7261 cgaaggcgga ctgatggccg aagatggctt ccagaacagc cttggcgttg ttgaccgcca
     7321 tgcccacgct cagtgtgaga aggaggggga ggtacggcag ttccttccac caggatttgg
     7381 gacgcaccac gatttgggca ctcatgtaaa agatgcagac ggccacgctg gtcatgaaaa
     7441 acagggccac attcacaaac cacatgaaga ccgtttcatt ttccggaatg cgctgcgtgc
     7501 agatggggta caccaggaag cagagcaatg ccaggagcag ataggaataa ttacaggtga
     7561 ggtgggtagt ggcttctacc ttggctttaa ggggggcatt ggagcgccag atgtccagca
     7621 ggattttctg gcagacctga atggaaccct tggtccaccg gtgctgctgg gacttgaacc
     7681 cgtccatgtc cacgggaagt tccgccgggg taacgacgtc gttcaggtag atgaagcgcc
     7741 agcccttgag ctggacacgg taggaaaggt ccatgtcttc cgtcagggtg tcatgggacc
     7801 agccgcccgc gtcgccaatc acgtatttgc gccatatgcc ggcagtgccg ttgaaagtga
     7861 agaagcggcc ggaacggttg cgagccgtct gttccatggc aaaatggccg tccaggtaca
     7921 tcccctggat gcgggtgagg agattgtatt cacggttgat gtggccccag cgggtttgaa
     7981 cgaggcccac gttttcatcc gtgaagaaat ggatggtttt ttgcaggaga tccggttccg
     8041 gaacgaagtc agcatccaga atcagcagga attccccctt cgctactttg gtagccgctt
     8101 ccagcgcgcc tgccttgaag ccagtgcggt ccgtgcggtg gatgcagacg gcgtcaaagc
     8161 cgcgggactt taattcctcc accttgcggt agcattgttc cgtggtgtcg tctgtagaat
     8221 cgtccagaat ctggatttcc agcttatcct gcggataatc cagagcggct acggattcca
     8281 gaaggcggtc cacgacgaac ttctcattga acatggggag ctggaccgtc acgacgggca
     8341 gttcctggaa gcgtgctttg ggctggggct tgttattacg gtttttccag tacaggtaaa
     8401 cgatgctcag gcggtggaag ccatatcctg ccaggccgac gaggaccaga agatagcaca
     8461 aaagccagat aaaattgtaa tttaaagaca tgcaggggtt acaaaggggg ttggtatctt
     8521 tttgtgtcag ggaaggggga tttccttgac gtgacgcccg tataggacac catagggcga
     8581 gagtcaagac atattccgtt ttgcttaacc tttttgagtg ttgttatgaa gagattgtat
     8641 ctgctggccg cagctgtatc cgtcacgggg ggaatggcgt tttccgctcc acaagaagat
     8701 gccccggaga aggaggcggc agtactggat gtgcaaacgc cgccgtccgg caagcaggac
     8761 cagccggcta ttccggaacg cctgctggac gactcccaca tgaatgagga gctgggcatc
     8821 aacgaattta ccgcgccgtc catccgcaag atttttgaag atttggaggg gcttcctgaa
     8881 ataccggaaa aggtggccct gcgcgcacgc ccggagcggc ctcccatgga ccgctgttcc
     8941 ctggctctcc agttgggcgg catgatggcg gacggcttcg taatcgtgca gtgcggcaag
     9001 atgaacgagg tgaaacccat tgccctggat ctttccagct atgcaaaggc cattggagcc
     9061 ggagaaaggg tgaaccgcca cgccgccagc ctgctgaaaa atgcggaaga cggggatttg
     9121 agcagtttca aaaacaacct ggcccttatt caatccgatg tggaacagga gcttgcctcc
     9181 ctgcgggatt ccgatatggc gaacctgatc ggcctggggg ggtggatacg cgccctggat
     9241 gccgctaccg cggctctgga taacaaattt gcggaggaca aggcgaaggt catcttccag
     9301 ccggatgtcc cggaatattt tcactatatt ctggacgggg tagagccgga aatgaaagaa
     9361 cgccgcgaca ttgccaaaat catgaacctt ctgacgatct tgcaggagca gatgacgctg
     9421 gctccggacg ccaaacccgc caaggagggc gtggaagcca tccgcaaaac gacgctggag
     9481 ttgatcaagg cggctctggc ccctgtggaa tcataattcc tctccggagg catgccgcat
     9541 atggttcttc cccttttaac cagtctggaa gtgcgtctta ctccgcgcgg cctgtacctg
     9601 cgggatgaaa aactgaaacg gatggtccgg gatgatggcc gcgttcccgt gccttacaac
     9661 gtaagcattt t
//
LOCUS       DAOMSU010000061         9183 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_509_length_9183_cov_2.759860, whole genome shotgun sequence.
ACCESSION   DAOMSU010000061 DAOMSU010000000
VERSION     DAOMSU010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9183
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_509_length_9183_cov_2.759860"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="PK077_04120"
     CDS             complement(<1..565)
                     /locus_tag="PK077_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23515.1"
                     /translation="MTTTPQTPFERLRAVLLPRKRIAVALSGGLDSSVLLAAAVRILG
                     ADHCLALTARTPYVMEEEVRDSTALCRRLGVRQEKLAFPILPALANNPPLRCYLCKHA
                     LFSSLAARAAEMGFPLLADGSNLDDLDDYRPGRKALQELGIPSPFLEASMDKEDIRQL
                     ACRLGLPESVSGKPAYACLLTRLEHNRP"
     gene            complement(537..1757)
                     /gene="larC"
                     /locus_tag="PK077_04125"
     CDS             complement(537..1757)
                     /gene="larC"
                     /locus_tag="PK077_04125"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HRN23516.1"
                     /translation="MRALVYDCSAGISGDMNLAALIDLGADRENLERELSKLHVHGEW
                     KLECRRAQQHGIRGTRIDVLTEEEGHSGSSHAHHHRTMADIRKLIETSALSNTVKRTA
                     LSIFTLLAEAEARVHGTTPEEVHFHEVGAVDSIIDIAGAAICLEMLRVDAVFTGPVEL
                     GSGTVTCQHGTMPVPAPATALLAKHFRATLNGTTHEATTPTGAAYIAALAQPVPAPLA
                     GRITATGYGIGHRQGLPVPNILRVMLVETEEEETSPELLTELCANIDDMTPEQTAYLA
                     EKLMEAGALDTWQESVCMKKGRLAVKVCALCQPEQTDRVREAFFRHSSTPGIRQHGML
                     RHILRRESSPVHTPHGTVHVKTSFMHGRPHYRKAEFEDCRILAEKTGFPLGQCQLMGL
                     FPTPSHDNDATDSV"
     gene            2026..2970
                     /locus_tag="PK077_04130"
     CDS             2026..2970
                     /locus_tag="PK077_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRN23517.1"
                     /translation="MKQVSEFVEAQSSCFDPEVADYMETVCQSKGKMLRPALVLLVAG
                     ATGGIKPAHIRLGALLEMVHLASLVHDDVIDEADKRRDEATANALWGNSLAVLLGDVL
                     FSHAMVLGTEFGSTEFCRRLANTVRNVCQGEVAQSSRLYDLSMTREEYFEIIRKKTAS
                     LFSAATGGAGWISGVTPEVEESLYQLGDLLGVAYQIYDDCLDMVGDEDDAGKTLHTDA
                     TKGKLTLPIFNLLECGDRNVEATLRDAIENREVVDYSAFQDNPVFAEALDKAIQVALE
                     KNEAAREILWLLPQTEYREALAEMTFYMDELLNDCRIS"
     gene            complement(3149..3802)
                     /locus_tag="PK077_04135"
     CDS             complement(3149..3802)
                     /locus_tag="PK077_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23518.1"
                     /translation="MEDTRHILKILSIFAFIIPFWSIFEQTASSWVSQGSRMIPLSIP
                     LPGGSWSIGPAQIQAANPIFVMVFIPLITVFVYPRAATLARPLVRLGTGLALSSATFL
                     IVAFLQYRLEEGTSMSIAWQLIPYCVLTISEILVSTTGLEFAYTQAPAHLKSLITSFW
                     NLTIFAGNMLVAAITFFLSNGESANAISTHRFILYAVLAAVVAVAYSFRARRYGKTE"
     gene            complement(3778..4491)
                     /locus_tag="PK077_04140"
     CDS             complement(3778..4491)
                     /locus_tag="PK077_04140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN23519.1"
                     /translation="MSALPSQSRYIIGTEACERFSFYGMKSILMLYMTGHLLMSDNWA
                     TSTLHIFVGMVYLLPLAGAWLADKVWGRYKTILYISLLYCVGHGVLATADLFHTIEAR
                     RYILMAGLFIIALGAGGIKPCVSAFMGDQIPNKSPQLMTKAFNAFYWAINLGSFFSFL
                     VIPAMEQRYGYSWAFAVPGLFMGVATFVFWLGRKKYHKTPPGSLASGKSFSSFCSTEA
                     GKTQNSAAELLPWKTPGTS"
     gene            complement(4639..6414)
                     /locus_tag="PK077_04145"
     CDS             complement(4639..6414)
                     /locus_tag="PK077_04145"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRN23520.1"
                     /translation="MLDYLRKHTIVIMAAMALVFVGLMFVGGDVSGGSLTGMFKQTFV
                     SVDGKSYGEKDYNNMGRTGLSVAFSFPSTFGPLLQDNFSSEENLRELCHLLKTNNPNA
                     AFLAYRGIIRREAGRLGLTPSTAEIDDAICNIPEFQDDKGVFDPQKYDNFISMRGNMG
                     KKLQEELLRGLMEDTISLERIKDVLAGGISVEPGFAEAVAESNSQQITINTAFLEKSA
                     FRPKTDPGEEEIKTFWDKHKENYKNEEARFFTVYTFIPAGDAQAPKPGDISNATMETM
                     NLVENDIWEPLNATNGRNMDQAISEALAKAPGVCKMEKKSYTAVTRKNAPQEINQAIN
                     QSASDGRSANLLDVAFSLAGAPALNADADAKAIEEARAKTGAEQISTMQVLENGQVVL
                     IRLEGITPVKALPYETARNSARADLLESMTVESINKAATELNTELQKAETPVEQFNAI
                     AGKAGAKTASYGPFVNPNVLLSSVNLQNAKTQEELQSLLERAMNSRLAPPKELPAPEE
                     VFQAASVINPGQMAQPINTGDGILLTQLVKRELEDTPEFQIKSTQQIAPALTAQAKSM
                     IMLDWLKACITQYKVEIAPILNQER"
     gene            6746..7291
                     /gene="sufT"
                     /locus_tag="PK077_04150"
     CDS             6746..7291
                     /gene="sufT"
                     /locus_tag="PK077_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HRN23521.1"
                     /translation="MLNQVVELKREVTAVQIPSGDVLTLPEGERVYITQILGGSYTVA
                     TDHGLARISRENADALGAGAEEPSPEAAALDENATLEERVWDTLKCVYDPEIPVDIVN
                     LGLIYDVAVIELENGLHHVAVKMTLTAPGCGMGPHLVMEAKDRIEALEGVEAADVEMV
                     WDPPWNQDMVSEEGRMKLGLI"
     gene            7298..7771
                     /locus_tag="PK077_04155"
     CDS             7298..7771
                     /locus_tag="PK077_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23522.1"
                     /translation="MPVTLKLSDEEARDLAEMLSTAATVAASNQQDGAEARLAAWGNL
                     VSRLMKELSVTSKLKGRIAYADELGGYAFTREYEESAFFQDCLDEYRDNSFWADLVTR
                     MADKAISEHLGPEYFENMPEDERRRTAEALEKSLWQECARYGIDRLGFILPPSDG"
     gene            complement(8004..8156)
                     /locus_tag="PK077_04160"
     CDS             complement(8004..8156)
                     /locus_tag="PK077_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23523.1"
                     /translation="MSPTGWLTMIVSIGGVLAAAAWAYYKVLSTPKETEYLGSPTPPL
                     PENAEE"
     gene            complement(8159..8659)
                     /locus_tag="PK077_04165"
     CDS             complement(8159..8659)
                     /locus_tag="PK077_04165"
                     /inference="COORDINATES: protein motif:HMM:NF023384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HRN23524.1"
                     /translation="MKEQADKASWTIPGQSGRTCASSSASKKWGNSRKSAFNNKSRSW
                     DLISKWKTHSSEKELSFSVGFVEDFMRNFESAKKARGAFFGPSFAYKDGKWHPNTSVT
                     FTGDPTPSTNVMANIQPDGSVLLQTGGTTKMTDFKLFQNRPLPQNIKLVLPDKAVTSL
                     VQENIN"
     gene            complement(8547..9035)
                     /locus_tag="PK077_04170"
     CDS             complement(8547..9035)
                     /locus_tag="PK077_04170"
                     /inference="COORDINATES: protein motif:HMM:NF023384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3472 domain-containing protein"
                     /protein_id="HRN23525.1"
                     /translation="MRLLSSAAVICAALFSGTILHAQDPELLAKRQCRSVHINQQGHP
                     SQASALYNVVKAKTSVPGTYFCAMNFDDGYIGFQEQSNGKKVIIFSIWDPVAHGDNPN
                     DVPEEERTKLVKLGKDARSGRFGGEGTGGQSFVDYPWAIGENMRFLVCVKKMGKFKEI
                     SV"
BASE COUNT         1918 a         2360 c         2809 g         2096 t
ORIGIN      
        1 cggggcggtt gtgttccagg cgggtcagca gacacgcata ggccggtttg ccgctgactg
       61 attccggaag acccagccgg caggccagct ggcggatatc ctccttatcc atggaagctt
      121 ccagaaaggg actcgggatg ccgagctcct gcaaagcttt gcggcctggt cggtaatcgt
      181 ccaggtcgtc gagattggaa ccgtccgcca gcagggggaa ccccatctca gccgctctgg
      241 ctgccaggga ggaaaacagg gcatgcttgc acaggtagca gcgcagggga ggattattcg
      301 caagggcggg aaggatggga aacgccagtt tttcctgccg gacgcccagg cggcggcaca
      361 acgccgtgct gtcccgcact tcctcctcca tcacgtaggg cgttcgcgca gtcagtgcca
      421 ggcagtgatc cgctcccagg atacggacgg cggcggcaag caacacgctg ctgtccagcc
      481 ccccggacaa ggcgacggca atgcgtttcc tggggagaag gacggcgcga agccgttcaa
      541 acggagtctg tggcgtcgtt gtcatgggaa ggtgtgggaa aaagccccat cagctggcat
      601 tgtcccagcg gaaatccggt tttttccgcc aggattctgc aatcttcaaa ttccgctttc
      661 cggtaatggg ggcggccgtg catgaaagaa gtttttacat gaaccgttcc atggggagtg
      721 tggacggggg agctttcccg gcgcagaata tggcggagca tgccatgctg cctgatgccc
      781 ggcgtgctgc tgtgccggaa gaaggcttcc cgcacgcggt ccgtctgttc cggctggcac
      841 agggcgcaaa ccttgactgc cagacggcct ttcttcatgc agacggactc ctgccaggta
      901 tccagagcac ctgcctccat cagcttttcc gccaggtagg ctgtctgttc cggcgtcata
      961 tcgtctatat tggcgcacag ctccgtcagc agttccgggg aagtttcctc ttcttccgtt
     1021 tctaccagca tcacccgcag aatattcggt acgggaagtc cctggcggtg gccgatccca
     1081 tagccggttg cggtaatacg gcctgccagc ggagcgggga cgggctgcgc cagggcggca
     1141 atgtaggcgg ctccggtggg tgtagtggcc tcatgggtgg ttccattcaa ggtggcccgg
     1201 aaatgtttcg ccagcaaagc cgttgccggc gcaggaacgg gcattgttcc gtgctggcag
     1261 gttacggtgc cagaccccag ctccaccggt ccggtgaaga cggcgtccac ccggagcatt
     1321 tccaggcaga tggcagctcc cgcgatatcg atgatggaat ccaccgcgcc cacctcatgg
     1381 aaatgtactt cttcaggagt agtgccgtgt acgcgggcct ccgcctccgc cagcagggtg
     1441 aaaatagaaa gggccgtccg ctttacggta ttggaaaggg cgcttgtctc aataagcttt
     1501 cgtatatccg ccatagtccg gtgatggtgc gcatggctgc tgccggagtg cccttcttcc
     1561 tccgtcagta cgtcaatccg tgttccccgg atgccgtgct gctgcgcccg gcggcattcc
     1621 agcttccact ccccgtgcac atgcaatttg gacagttccc gttccaggtt ttcccggtcc
     1681 gctcccaaat caatcagggc ggccagattc atatctccgc tgatgcccgc gctgcaatca
     1741 tagaccaagg ctctcatgat ggctcagtgt agaaggaaaa actcctggct caaggtcaaa
     1801 atgagcctta tcccgggagg gggcccggtg cttggcggac cgggtttggc ggattgccgt
     1861 ccggttatga gaaagatgcc ctgtgaaata cattgctagg gaactttaaa tatgccacaa
     1921 tccctgcggc gctgaccagc gccgcccccg ttctatatca tgtccattcc gttcttttcg
     1981 aaattacgat cccagaagca ttatcttcaa ctggttaagc cggaattgaa gcaggtttcc
     2041 gagtttgttg aagcgcaatc atcatgtttt gacccggaag tggccgatta catggaaacg
     2101 gtatgccagt ccaagggtaa aatgctccgt ccggccctgg ttctgctggt tgccggtgct
     2161 acgggcggca tcaagcctgc ccatatccgc ctgggtgctt tgctggaaat ggtgcatctg
     2221 gcttcccttg tgcatgatga cgtgattgac gaggccgaca aacgccggga cgaggctacg
     2281 gcgaatgccc tctggggcaa cagcctcgcc gtactgctgg gggatgtcct cttttcccac
     2341 gccatggtgc tgggtacgga attcggcagc acggaattct gccgcaggct ggcgaatacc
     2401 gtcaggaacg tatgccaggg ggaggtggcc cagtccagcc gcctgtatga tctgagcatg
     2461 acccgcgagg aatattttga aatcatccgg aaaaaaaccg cttccctttt ctccgcagct
     2521 acgggggggg ctggctggat ttccggagta accccggaag tggaggaatc cctttaccag
     2581 ctgggggatc tgctgggggt agcctaccag atttacgacg actgcctgga catggtggga
     2641 gatgaagacg atgctggaaa gacgcttcat acggacgcca ccaagggcaa gctgaccctt
     2701 cccatcttca atttgctgga atgcggtgac aggaatgtgg aagccactct gcgggacgcc
     2761 attgaaaacc gtgaagtggt ggattactcc gcctttcagg acaatcccgt ttttgcagag
     2821 gctctggaca aggcgattca ggtagctctg gagaaaaacg aggcggcccg cgaaattctg
     2881 tggctccttc cccaaacgga atatcgtgaa gctttggcgg aaatgacctt ttacatggac
     2941 gagctgctta acgactgccg tatctcctga cctgcttcaa ggagtttttg tccggtcatg
     3001 tcctccttat gtaatttgtg tcttccggca ttttcccaaa aggaaacctg ccggagggaa
     3061 tgggaggagc gcatgctggt tacggtctga ttgttttttc aaaacatggt taacccggcc
     3121 acgcgcccgg ggaacggcag tgctttcttt attccgtttt tccgtacctg cgcgcccgga
     3181 aggagtaggc gaccgccacc acggcggcga gcacggcgta caggatgaag cggtgagtgg
     3241 aaatggcgtt ggctgattct ccgttggaca ggaaaaaagt aattgcggcc accagcatgt
     3301 tgcctgcaaa gatagtgagg ttccagaaac tggtgatgag gctttttaaa tgcgccgggg
     3361 cttgcgtata ggcgaattcc aggccggtgg tgctgaccag gatctcagag atggtgagta
     3421 cgcagtaagg aatcagctgc catgcgatgg acatggaggt tccttcctcc agccggtatt
     3481 gcaggaaagc gacaatcagg aatgtagcgg agctgagggc cagtcccgtt ccgaggcgca
     3541 ccaggggcct tgccagcgtt gccgccctgg gataaacaaa tacggtgatg agggggatga
     3601 acaccatgac gaaaatggga ttggccgcct ggatttgggc cggcccgatg gaccaggaac
     3661 cgcccgggag cgggatggaa agaggaatca tcctgctgcc ctgggatacc caggaagaag
     3721 ccgtctgttc gaaaatggac cagaatggga tgataaaggc gaagatggag aggattttca
     3781 ggatgtgccg ggtgtcttcc acggcagaag ttccgcagcg ctgttctgcg tttttccagc
     3841 ctccgtggaa cagaatgatg aaaaggactt tccagaagcc aggctgcccg gaggcgtttt
     3901 gtggtatttt ttgcggccca gccagaagac gaaggtggca actcccatga agaggcccgg
     3961 gacggcaaac gcccaactgt acccgtaacg ctgttccatg gccggaatga ccaggaagga
     4021 gaagaaggag ccaaggttga tagcccagta gaaggcattg aaagccttgg tcattaactg
     4081 cggggactta ttaggaatct ggtctcccat aaaggcggag acgcatggct tgatgcctcc
     4141 ggctcccagc gcgatgatga acaggcccgc catgagaatg taacggcgcg cttcaatggt
     4201 gtggaaaaga tccgccgtcg ccagaactcc gtgtcctacg cagtaaagca gggaaatata
     4261 aagaatggtt ttatatcggc cccagacctt gtccgccagc caggcccccg ccaggggaag
     4321 cagataaacc attcccacaa aaatatgaag ggtggaggtg gcccagttgt cgctcatcag
     4381 cagatggccc gtcatgtaca gcatgaggat ggacttcatg ccgtagaagc tgaaacgttc
     4441 acaggcttcc gttccgatga tgtatctgga ctgggagggc agggcgctca ttggatgatg
     4501 aaaaggaggg agatggagaa ggagtaataa aaatgatggc ctccgggcgg aggccatcat
     4561 ggtgaatgcg ttcagacggt gttcagggcc tgtgagaagg agacgggctc actgacggtt
     4621 gtcatgaaag ggaaaacgtt agcgttcctg gttcaggatg ggagcgattt ccaccttata
     4681 ttgggtgatg cacgctttca gccagtccag catgatcatg gattttgcct gggctgtcag
     4741 agccggagca atttgctggg tagatttgat ttggaattcc ggcgtatcct ccagttcgcg
     4801 cttgacgagc tgggtcagca ggatgccgtc ccccgtgttg atgggctgag ccatctggcc
     4861 ggggttgatg acggaagccg cctgaaatac ttcttcaggg gccgggagct ctttgggagg
     4921 ggccaggcgg gaattcatgg cccgttccag aagagactgg agctcttcct gtgttttggc
     4981 gttttgcagg ttgacgctgc tcaagagtac gtttggattg acaaagggac cgtaagaggc
     5041 agttttggca ccggcttttc cggcaatggc attgaactgt tccacggggg tttccgcctt
     5101 ctgcaattcc gtattgagtt ccgtggcggc cttatttatg gattcaacgg tcatgctttc
     5161 cagcaggtcg gcgcgggcgc tgttgcgggc ggtttcgtaa ggcagggctt tgaccggagt
     5221 aatgccttcc agacggatca ggacgacttg tccgttttcc agcacctgca tggtgctgat
     5281 ctgttcggct ccggtcttgg cgcgcgcttc ttcaatggcc ttggcatccg catccgcatt
     5341 cagggccggg gctcccgcca gggagaaagc cacatccagc agattggcgc tgcgtccgtc
     5401 ggaagcggat tggttgattg cctgattgat ttcctgaggc gcgtttttac gcgttacggc
     5461 cgtgtaggac tttttctcca ttttgcagac gccgggagcc ttggccaggg cttcgctgat
     5521 ggcctggtcc atgttcctgc cgttggtggc gttcagcggt tcccagatgt cattttccac
     5581 cagattcatg gtctccatgg tggcgttgga aatgtctccg ggcttgggtg cctgggcatc
     5641 gcctgcggga atgaaggtgt aaacggtgaa gaagcgagct tcctcatttt tgtaattttc
     5701 cttgtgcttg tcccagaagg tttttatttc ctcttctccg ggatccgtct tggggcggaa
     5761 ggcgcttttt tccaggaagg cggtgttgat tgtaatttgc tggctgttgg attccgctac
     5821 agcctcggca aaaccgggct ccacggagat acccccggcc agcacgtcct tgatgcgctc
     5881 caggctgatg gtgtcttcca tcagaccgcg cagcagttcc tcctgaagct ttttacccat
     5941 gttgccacgc atggaaatga aattatcgta tttttggggg tcgaaaacgc ctttatcgtc
     6001 ctggaactct ggaatattgc aaatggcatc gtcaatttcc gcggtgctgg gagtcaagcc
     6061 caggcgtccc gcctcccggc ggataatgcc gcggtaggcc aggaaagccg cattgggatt
     6121 atttgttttc agcagatggc agagttcgcg caggttttct tccgagctga aattgtcctg
     6181 aagcagcgga ccgaaggtgg aagggaagga gaaagccacg gacaggccgg tcctgcccat
     6241 attgttataa tccttttccc cgtaagattt cccgtccacg gaaacgaaag tctgcttgaa
     6301 catgccggtc agggagccgc cgcttacgtc cccgccgacg aacatgaggc ccacaaacac
     6361 cagggccatg gctgccatga tgacgattgt atgtttacgt aagtaatcta gcatggaatg
     6421 tcaggttgaa aatgtacgtg tagccgacca tgagctgacg gcagcccggg cagcccgaac
     6481 cttttacact ttttccctcc gctaggcaag aacgaagctc tccgcttccg gcggcttctc
     6541 cagaatatgg cggcatgctc tttcctgtca tgaagggctt ttccggccgg ggctgaacaa
     6601 tttttgacct cgacggtgat gcaaatacgc cttatgttgc tcctgcgcgg aaggttccgg
     6661 gaaccatggg ccggttctgc cccatggcat tcggaagcct tcggtgcatg tccttagccg
     6721 gatattcctt taattgcaag gatttatgct gaaccaggta gtagaactca aaagagaagt
     6781 gacggcggtc cagatcccca gcggggatgt gctcaccctg ccggaagggg aacgcgtgta
     6841 tatcacccag atactgggcg gctcctatac cgtggctacg gaccacggcc tggcccgtat
     6901 ctccagggag aacgcggacg cccttggcgc tggagcggag gaaccttctc cggaagcggc
     6961 tgcgctggat gagaacgcca cactggaaga aagggtctgg gatacgctga aatgtgtgta
     7021 tgacccggaa attcccgtgg acatcgtcaa cttgggtctg atttacgatg tggccgtcat
     7081 tgagctggag aacggccttc atcatgtggc ggtaaaaatg actctcacgg cgcccggctg
     7141 cggaatgggt ccccatctgg tcatggaagc caaggaccgc attgaagcgt tggaaggggt
     7201 ggaggctgcg gatgtggaaa tggtgtggga tcccccctgg aaccaggaca tggtcagcga
     7261 ggagggcaga atgaaactcg gattgatttg atttcccatg ccggtgacgt tgaaattgtc
     7321 cgacgaggaa gcgcgtgatt tggcggaaat gctttccact gccgccacgg tggctgcctc
     7381 caaccagcag gacggggcgg aggcgcgtct ggcggcgtgg gggaatttgg tttcccggct
     7441 gatgaaagag ctttccgtca cctccaaatt gaaagggcgc attgcctatg cggatgagct
     7501 gggcggatat gccttcaccc gggaatatga agaaagcgcc tttttccagg attgcctgga
     7561 tgaataccgg gacaactctt tttgggcgga tctcgtgacg cgtatggcgg acaaggccat
     7621 cagcgaacac ctggggccgg aatattttga aaacatgccg gaggatgaac gccgccgtac
     7681 tgcggaagcc ctggaaaaat ccctgtggca ggaatgcgcc agatacggca ttgaccggct
     7741 gggcttcatc ttgcctccgt cagacggata aggagaagaa ccgtttaaaa atcctgtgga
     7801 ccggtgcggc ccgaatgatt cgggcgcgat tcgcgtgcat atccccggag aataccggtc
     7861 tatgttgcct gtctgccggg aaatactccc ttatatccgc cctgtttcag gtatcgggac
     7921 gacatgcgct ctctctggat gcggcaagat gattttgggg gagttgttca gtccggagtg
     7981 cagcggttca ccgggtcggg gccttattcc tccgcatttt ccggcagtgg cggggtgggg
     8041 ctgcccagat attccgtttc cttgggggtg gagagtactt tgtaatacgc ccaagcggcg
     8101 gcggccagta cgccgccgat ggaaacaatc atggttagcc agccggtggg ggacataatc
     8161 agttaatgtt ctcctgaaca agactggtaa cggccttgtc aggcaggacc agcttgatat
     8221 tctggggcag gggacgattt tggaacagct tgaaatccgt cattttcgta gttccccccg
     8281 tctgaagcag cacggaacca tcaggctgga tattcgccat gacgttggtg gagggggttg
     8341 ggtcgccggt gaacgttacg ctggtattgg gatgccattt gccgtccttg tacgcgaagc
     8401 tggggccaaa gaaggctccg cgggccttct tggctgattc aaaattacgc atgaaatctt
     8461 ccacgaatcc gacggagaag gaaagttcct tttccgagga atgcgttttc catttggaaa
     8521 taagatccca ggacctgctt ttattattaa acgctgattt ccttgaattt ccccattttt
     8581 ttgacgcaga cgaggaagcg catgttctcc ccgattgccc agggatagtc cacgaagctt
     8641 tgtccgcctg ttccttcacc gccaaaacgt ccggagcgcg catctttgcc cagttttacc
     8701 agcttggtgc gttcttcttc cggcacatca ttggggttgt ctccgtgtgc caccggatcc
     8761 cagatggaga agatgattac ttttttaccg ttggattgtt cctgaaaacc gatatagccg
     8821 tcgtcaaaat tcatggcgca aaagtaggtg ccgggaacgg atgttttggc tttcactaca
     8881 ttgtacaggg cgcttgcctg ggagggatgc ccctgctggt tgatatggac ggaacggcac
     8941 tggcgcttgg ccagcagttc cgggtcctgc gcatgaagga tggttccgga gaacagggcg
     9001 gcgcaaatga cggcggcgga ggataaaaga cgcatggttt tgaatgattg atggttgtct
     9061 gatttgatgc ccggagggtg ggggacaggc aaataattag gacaggctaa ttacgtttgg
     9121 cctgggggaa atctcgcagg caggccgcac ttttttcaaa atttttccgg catgaatgtg
     9181 ata
//
LOCUS       DAOMSU010000062         9164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_511_length_9164_cov_3.179603, whole genome shotgun sequence.
ACCESSION   DAOMSU010000062 DAOMSU010000000
VERSION     DAOMSU010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_511_length_9164_cov_3.179603"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..172
                     /locus_tag="PK077_04175"
     CDS             <1..172
                     /locus_tag="PK077_04175"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRN23526.1"
                     /translation="AFGPGKVVYVSCNPATQIRDLAEFDKAGYSVTAVQPFDLFPQTK
                     HLECVVTLKKNT"
     gene            181..1557
                     /gene="miaB"
                     /locus_tag="PK077_04180"
     CDS             181..1557
                     /gene="miaB"
                     /locus_tag="PK077_04180"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRN23527.1"
                     /translation="MPKLYIKTYGCQMNERDSEQVARMFVQKGYTMTDREDEADVILF
                     NSCSIREQAEQKALGKMGLLAKQQRHRPHVVYGMMGCMAQSKKEELFKELPRLDLVVG
                     TQKYHRVFEHVDGILRARQERRMDELQTAFSGTHVCDVAEEEDSQNRIRDHLNPGVRS
                     TAYVSIMQGCEMKCAYCIVPYTRGAERSRPIRDVVDEVKMLADAGVKEVTLLGQIVNR
                     YGRQMETAGGKGGFVQLLEAVHEVEGIRRIRFVSPHPIGFRQDLVQAFTYLPKLCSHI
                     HFPMQSGSDRILKMMRRPYRNETYLDLCSQMKQARPDLSITTDIIVGFPGETEEDYLL
                     TRQAVEQVQFDNAFIFRYSPRRGTPAAVMENQIPEEVKEARNQDLLAVVNEIAIRKNR
                     DLVGTVQEVLLEGPSKTNAARLSGRTSQNKPVMVDAAPDLAGEILPIRIEESTGFTLY
                     GVPCPSRG"
     gene            complement(1520..3529)
                     /locus_tag="PK077_04185"
     CDS             complement(1520..3529)
                     /locus_tag="PK077_04185"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRN23528.1"
                     /translation="MDVSLWFWLPCFCLASLPCFLRAPSMGVLAAALAMWAAVYLFAY
                     EREYAGVPVEAGRAMVMEGSVEAVSGKSVLFRPDGSRWLYAVSSRDGACPLEAGEHYR
                     IRGEAYPLQAPCGPGIFDRERWGYLHGIIAGIRLDEYCRNGPGDWRSRFRAFSLRLRE
                     RAASLLREGAPPDDEARQVMVSAVLGDKTDARPETMAKFLESGCMHVFAVSGMHVGVA
                     AMLVLGMLRLLHVYPRAARLACILLLAAYVFVTGMSASALRAFIMATVWLLAFVLRRK
                     GHPANILALAFILLCLMNPLQVFQPGFQLSFCVFAVIVCLAEWLSREKPLWSPDPFIP
                     PRIYHARERFLVRLEKACRGALIVSVGAWLASIPLTAWHFGTWNLYAPLTNLCLSVLV
                     FPLMGISLSGLMFAWCPWMLHVCNTAASWLASAMLSVAGGVASLPYSYLSSAPPSGEN
                     EAVIVPLQKDAWATVISNPALVVGSGTEDAVRYTLLPVLKARSIRPCGMLSWGNGKAE
                     RAGEKELEKEYPGIRNWGAEFGECPVRQWRLRPGNALVLEDFPVPLRTGIRQDRNRVL
                     SWECRGRRFLMIGNTGFSSLARAEKTPRADVLLIGHHPRDPVDSAAWIRATGARAVIF
                     TTEHECPVPKGTAVYRLRETGTLYLRMEEKSVQIIPWRGMERRTE"
     gene            complement(3644..4699)
                     /locus_tag="PK077_04190"
     CDS             complement(3644..4699)
                     /locus_tag="PK077_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRN23529.1"
                     /translation="MEQVSLMELGKLAAEGQVEAEVFAQIAQCAQKMTKGNKPYLDVS
                     FADAEGTMGLKVWEDKPWFRTLASLPLRSFVSLRGQWAKGGFGMEVADLDVRLLDEQE
                     KENFLAGSGTLKKKQEADLKEICMFIKGMNDPRIRALCIEFIEQFGERLQRAAAARTY
                     HHARRGGLVEHVAGMMRTASAVCQANPELNRDLLLAGCLFHDCGKLWENCYPKEDFTM
                     PYSEAGELLGHIPLGIELVNNLWKRIMSLPEADSWKTLDPPSPDVRMHLLHLIASHHG
                     ELAFGSPVFPKTPEAVALHYIDNLDAKLEMFRGAYETSEALAPRVLQRKAPLPANVVL
                     PLPSVLPLEPDGEDALP"
     gene            complement(4825..5799)
                     /locus_tag="PK077_04195"
     CDS             complement(4825..5799)
                     /locus_tag="PK077_04195"
                     /inference="COORDINATES: protein motif:HMM:NF015715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HRN23530.1"
                     /translation="MIQDFANWLVYKILEFTPHTQLGEAVNFFVYDTVKILFLLYAIS
                     LIMGVVNAYFPVERIRVFLITRKLYGLQYFFAALFGAMTPFCSCSSIPLFIGFVKGGI
                     PLGVTFAFLITSPLVNEVAIAMFLGAFGIKITVIYAVSGILLGMVGGIVLEKFKLERY
                     LSPWIRDLLVQSNAESNDWERKKTPFMGKLPVIMRDAWGIVKGVTLYVVIGIAIGAAV
                     HGYVPAGFFEESMSRENWWSVPVAVICAVPMYANAAAIVPVIEVFVAKGVPIGTAIAF
                     MMATVGLSLPEAALLKKVMTWKLIGIFFGTVTAFIILSGYVFNLLISC"
     gene            complement(5828..6514)
                     /locus_tag="PK077_04200"
     CDS             complement(5828..6514)
                     /locus_tag="PK077_04200"
                     /inference="COORDINATES: protein motif:HMM:NF040495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic aminobenezylarsenical efflux permease
                     ArsG family transporter"
                     /protein_id="HRN23531.1"
                     /translation="MDWPLLSLLPIGVAAAVSPCPLATNIAAVGFISRGIGSRHQSAI
                     ASLLYTLGRVSAYVLIGWLVSRGLASVPMLSFWMQEELPVYLGPVMMVAGLILLGFIP
                     FFSLGKKPGEATAQSLMAKGGIWGAFALGFLFALALCPPSAALFFGTAVPLSIQAGST
                     GSLLGISLFGIGTALPVVAFALLLTFSAEKAAAAVRHMPKIQQIMKLVTGWFFLLLGG
                     YWLFSRILLV"
     gene            complement(6545..7378)
                     /locus_tag="PK077_04205"
     CDS             complement(6545..7378)
                     /locus_tag="PK077_04205"
                     /inference="COORDINATES: protein motif:HMM:NF040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH895/ArsE family thioredoxin-like protein"
                     /protein_id="HRN23532.1"
                     /translation="MNNIVVYGPGCAKCTALADVTKQAMQELHLDASLEKVTDAMQFA
                     VAGVLVTPALAVDGKVFVSGRVPSKDEIKKILQETIQADSSRQKSCCCSGVAESALPQ
                     ENDSHAKEVSCCGGSCCSSEASKGCSGWKKAVVWVVALLILLAIVKVINHQGAENREE
                     PAATTVTMKSGVEAVYYEYGARCPTCIRMETWTKEAIEKNFPIQLKEGKLAFRAIPAD
                     KDAVGKYELTTKSLLLKDWKDGKETRWVDLDRIWDLSGNEQEFKEYVVQSIRKALDDA
                     H"
     gene            7841..>8055
                     /locus_tag="PK077_04210"
     CDS             7841..>8055
                     /locus_tag="PK077_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23533.1"
                     /translation="MARRANTVSYGNSLENYPHAGQVNSLPICSLAYFPATAMLHIGC
                     HLSSAKGYEATGRVALSISANTFQFFTR"
     assembly_gap    8056..8065
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8066..8789
                     /locus_tag="PK077_04215"
     CDS             <8066..8789
                     /locus_tag="PK077_04215"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRN23534.1"
                     /translation="GSKAKAIDEQDIARFLELARNNGFGTLLAHAPYTLNPCSADPGV
                     ARFAAQVLKEDLELMEHLPGNLYNFHPGCHVGQGAEKGIELVAGQLNGVLSPEQTTIV
                     LLETMSGKGSEVGRTFEELATIMERVDLKDKLGVCLDTCHVYSAGYDIVNGLDSVLEH
                     FDAVLGLERLRAIHLNDSMTPFSSFKDRHETIGKGSLGEQAFINIINHPVLRELPFFL
                     ETPRDDAGHGEEITWLKEHYRN"
     gene            8953..>9164
                     /gene="eno"
                     /locus_tag="PK077_04220"
     CDS             8953..>9164
                     /gene="eno"
                     /locus_tag="PK077_04220"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420224.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRN23535.1"
                     /translation="MRIAKITGREILDSRGNPTIEADVYLEGGAVGTAGVPSGASTGE
                     HEAWELRDGDATRYQGKGVTKAVDNIR"
BASE COUNT         2264 a         2704 c         2409 g         1777 t
ORIGIN      
        1 tgccttcggc cccggaaaag tcgtttacgt ttcctgcaat cccgccaccc agatacggga
       61 tctggcggaa ttcgacaagg ccgggtattc cgtcaccgcc gtccagccct ttgacctgtt
      121 cccccagacc aagcatctgg aatgcgtggt caccctgaaa aaaaatacct gattttttcc
      181 atgcccaagc tttatatcaa aacctacggc tgccagatga acgaacggga ctccgaacag
      241 gtggcccgca tgttcgtgca gaagggctac accatgacgg accgcgagga tgaggcggac
      301 gtcatcctgt tcaactcctg ttccatccgg gaacaggcgg aacaaaaggc tctgggaaaa
      361 atggggcttc tggccaagca gcagcggcac cgtccgcatg tagtgtacgg catgatgggc
      421 tgcatggccc aaagtaaaaa agaggagctg ttcaaggaac tgccgcgcct ggacctcgtg
      481 gtcggcaccc agaaatacca ccgcgtattt gaacatgtgg acggcattct gcgcgcgcgc
      541 caggaacgcc gcatggatga gttgcaaacc gccttttccg gtacgcatgt gtgcgatgtg
      601 gcggaagagg aggattccca gaaccgaatc cgggatcacc tgaatcccgg cgtgcgctcc
      661 acggcgtatg tctctatcat gcaggggtgc gaaatgaagt gcgcctactg cattgttccc
      721 tacacccgcg gcgcagaacg cagccgcccc atccgggacg tggtggatga ggtgaagatg
      781 ctggcggacg ccggcgtgaa ggaagtcacg ctgctgggcc agatcgtcaa ccggtacggc
      841 aggcagatgg aaacggcagg cggcaagggc ggcttcgtcc agctgctgga agccgtgcat
      901 gaagtggaag gaatccggcg catccgcttc gtttccccgc atcccatcgg tttccgccag
      961 gatctggtgc aggcgttcac ctaccttccc aagttgtgca gccacattca tttcccgatg
     1021 cagagcggca gcgaccgcat cctgaaaatg atgcgcaggc cgtacaggaa tgaaacctat
     1081 ctggaccttt gctcccagat gaagcaggca cgcccggatt tgtccatcac cacggatatc
     1141 attgtgggct tcccaggaga aacggaggag gattacctgc tgaccaggca ggccgtggaa
     1201 caggtccagt ttgacaacgc attcattttc cgctattccc cgcgccgcgg cacacccgcc
     1261 gccgttatgg agaatcagat tccggaggaa gtgaaggaag cgcgcaacca ggatcttctg
     1321 gccgtagtca atgaaatagc tatccggaaa aaccgggatc tggtaggcac cgtgcaggaa
     1381 gttcttctgg aagggccgtc caaaacgaat gcggcgcgcc tctccggccg gacctcccag
     1441 aacaagcccg tcatggtgga tgccgcccct gacctggcgg gagagatact tcccatccgc
     1501 attgaggaaa gcacgggatt tactctgtac ggcgttccat gcccctccag gggataattt
     1561 gcacgctctt ttcttccatc cgcagataga gggttccggt ttcccggaga cggtatacgg
     1621 ctgttccctt cggtacgggg cattcatgct ccgtcgtgaa aatgaccgcg cgggcgcctg
     1681 tggccctgat ccacgccgcg ctgtccaccg ggtcgcgcgg atgatgcccg atgagcaata
     1741 cgtccgcccg cggtgttttc tccgcacgcg ccagggaaga aaacccggta ttccctatca
     1801 tcaggaaccg ccgcccgcgg cattcccatg acaacacccg gttccggtcc tgacggattc
     1861 ccgtgcgcaa cgggacagga aaatcttcca gcacaagtgc attgcccggc cgtaacctcc
     1921 attgccggac gggacattcc ccgaactccg cgccccagtt ccggattccg ggatattcct
     1981 tctccagttc tttctctccg gcccgttccg ctttgccgtt tccccaggac agcatgccgc
     2041 agggccggat actgcgcgcc ttcagcacgg gaaggagggt gtaccgcacc gcatcttccg
     2101 ttccgcttcc cacaaccaat gcgggattgg aaatgactgt ggcccaggca tccttctgca
     2161 agggaacgat cacagcttca ttttccccgg aaggcggtgc ggaagaaaga taactgtacg
     2221 gcagggaagc cactcccccg gctacggaca gcatggcgga cgccagccag gaagcggccg
     2281 tattacagac atgcagcatc cacgggcacc acgcgaacat cagcccggac agggaaatgc
     2341 ccataagggg aaagacaagt acgctcaggc acaaattggt caagggagca tacaggttcc
     2401 aggttccgaa gtgccatgcc gtcaggggaa tggaagccag ccacgccccc acggagacaa
     2461 tcagcgcgcc gcggcatgct ttttccagcc gcaccaggaa tctttcccgc gcatgataaa
     2521 tccggggagg aataaacggg tccggcgacc acaggggctt ttcccggctg agccattccg
     2581 ccaggcagac aatgacggca aaaacgcaaa aagaaagctg gaaccccggc tggaaaacct
     2641 ggagcgggtt cattaaacag aggaggatga aagcgagcgc cagaatattg gccggatgcc
     2701 cctttctgcg cagcacaaac gccagaagcc agactgtcgc catgatgaac gcccgtaggg
     2761 cggaagcgga catcccggtc acgaacacgt acgccgccag caaaaggatg caggccagcc
     2821 gggccgcacg agggtacaca tgcaaaagcc tcagcatacc cagaaccagc atggcggcca
     2881 cgcccacatg catgccgctg acagcgaata catgcatgca gccgctttcc agaaacttcg
     2941 ccatcgtttc cggcctcgcg tccgtcttgt ccccaagcac ggcggaaacc atcacctgcc
     3001 gggcctcatc atccggaggg gcgccctccc tgagcaggga ggcagccctt tcccggagcc
     3061 gcagggaaaa agcccggaaa cggctgcgcc agtctcccgg cccgttccgg cagtactcat
     3121 ccagccggat tccggcgatg atgccgtgca ggtaacccca ccgctcccgg tcaaagatgc
     3181 cgggaccgca cggcgcctga agcggatacg cctctcccct gatccggtaa tgttcccctg
     3241 cctccagagg gcaggccccg tcacgggagg aaaccgcgta cagccaccgg gaaccatccg
     3301 ggcgaaacaa tacggatttt ccggaaacag cctcaacgga gccttccatc accatggctc
     3361 tccccgcttc cacgggaacg cctgcgtatt cccgttcata cgcgaaaaga tatacggcgg
     3421 cccacatcgc cagggcagcg gcaagcaccc ccatggaggg cgcacgtaaa aaacacggca
     3481 gcgacgccag gcagaagcat ggaagccaga accaaaggga cacatccatg gccgcgcaag
     3541 cgcacagcag ggacaaagcc ggaatcagca agggagcaga tgccgtcatc cgttccggcc
     3601 acggacgaag aacacgccga acctcttcca tgcaggggga accttacggc agagcgtctt
     3661 cgccgtccgg ctccagaggg agaacggaag gcagcggaag aacaacgttg gcaggcaggg
     3721 gggccttgcg ctggagcact ctgggagcca gggcctcgct tgtctcataa gccccgcgga
     3781 acatttccag cttggcgtcc agattgtcga tgtaatgcag ggccaccgcc tccggcgtct
     3841 tgggaaacac gggagaaccg aaggcaagtt ccccgtgatg ggacgcaatc aggtgcagca
     3901 ggtgcatgcg cacgtccggt gaaggagggt ccagcgtctt ccaggaatcc gcctccggaa
     3961 gggacatgat gcgcttccac aaattgttga ccagttcaat gcccagcgga atatggccca
     4021 gcagttctcc ggcctccgaa taaggcatcg taaaatcctc cttcgggtag caattttccc
     4081 acaacttccc gcagtcgtgg aacagacacc cggccagcag aaggtcacgg ttcagctccg
     4141 gattcgcctg gcagacggca gaggccgtgc gcatcatccc cgccacgtgt tccaccagtc
     4201 cgccgcggcg ggcgtgatgg taggtgcggg cggcggcggc gcgctggagg cgttccccga
     4261 actgctcaat gaattcaatg cacagggcgc gtatgcgcgg atcattcatg cccttgatga
     4321 acatgcagat ttccttcaga tcagcttcct gctttttctt caaggttccg gagccggcca
     4381 gaaagttttc cttttcctgt tcgtccagca accgcacgtc caaatcggca acttccatgc
     4441 cgaaccctcc cttggcccat tggccgcgca ggctcacaaa actgcggagg ggcagcgaag
     4501 caagcgtccg gaaccagggt ttatcctccc acaccttcaa tcccatggtc ccttccgcat
     4561 cggcaaaaga cacatccaga tacggtttgt tgcccttggt catcttctgc gcgcattggg
     4621 caatctgggc aaaaacttcc gcttcaacct ggccttctgc ggccagcttg cccaattcca
     4681 tgagactgac ttgttccatg cgtgccatct tgcccctctt ccctccatcc gtcaagaaaa
     4741 cgcgggagga ctacgataat aaaaaaagga aaccgcttat tttcccaata ttcatatgga
     4801 ctaaataatc caatgacggt tttcttaaca agaaatcagt aaattaaata cataacctga
     4861 caaaatgata aacgccgtga ccgttccgaa gaaaataccg atcaacttcc aggtcatcac
     4921 ttttttgaga agggcggctt ccggaaggga aagtcctact gtcgccatca tgaatgcaat
     4981 tgccgttccg attggtactc cctttgcgac aaatacctcg atcaccggaa caatagccgc
     5041 agcatttgca tacatgggta ccgcacagat gacagcgaca ggcaccgacc accaattttc
     5101 ccggctcatg gattcttcga agaatccggc aggcacatag ccgtgcactg ccgccccaat
     5161 ggcaatgccg attaccacgt acagggtcac tcccttgacg attccccagg catcgcgcat
     5221 gataacgggc aatttgccca tgaagggtgt ctttttccgt tcccagtcat tgctctctgc
     5281 attcgactga accagcaagt cacgtatcca gggagacaaa taacgttcta gcttgaattt
     5341 ctccaggact atacccccga ccatgcccaa caggatgccg gaaacagcat agatcaccgt
     5401 aatcttgata ccgaatgctc cgaggaacat ggcgatcgcc acctcattaa ccaaaggaga
     5461 cgtaatcagg aaggcaaacg tcacccccaa gggaatgcca cccttcacaa atccgatgaa
     5521 caagggaatg gaggaacagg aacaaaatgg cgtcatggct ccgaacagag ccgcgaagaa
     5581 atattgaagc ccgtaaagct tcctcgtaat cagaaatacg cggatgcgtt ctacagggaa
     5641 ataggcgttt accaccccca tgatgaggct gatcgcgtat aacaggaaaa gaatcttgac
     5701 cgtatcgtac acgaaaaagt tgaccgcctc ccccagttgt gtgtggggag taaattccaa
     5761 tatcttgtaa accagccagt tggcaaaatc ttgaatcata taataagaaa gggtaatgta
     5821 gaaagaatca aacgagaaga atccggctga acagccagta cccgccaagc aacaggaaga
     5881 accatcccgt cacaagtttc atgatttgct ggattttagg catgtggcgc acggcagcag
     5941 cggctttctc cgcactgaat gtcagcagta aggcaaatgc gaccacgggc agggccgttc
     6001 ctatgccgaa cagggaaatt cctaataggg aaccagtgct cccagcctgt atggacaaag
     6061 ggacagccgt tccgaaaaac agcgccgccg aaggagggca aagagctagg gcgaacagaa
     6121 aacctagcgc aaacgccccc cagatgccgc ctttagccat aaggctttga gctgttgcct
     6181 ctcccggttt tttccctagg gaaaagaacg ggatgaaccc cagaaggatc agccctgcca
     6241 ccatcatcac cgggcccaga tacaccggca gttcctcctg catccagaat gacaacatgg
     6301 ggaccgatgc caagccccgg cttaccaacc acccgatcag cacataagcg ctcacacggc
     6361 ccaaagtata gagaagtgag gcgatggctg attgatgacg ggagccgatg ccacgggaaa
     6421 tgaatcccac agcggcaata ttcgttgcca ggggacaagg agaaacggct gccgctacgc
     6481 ctataggcag cagagataaa agaggccagt ccatcacgga aaatattaga aggaaggtaa
     6541 acgttcaatg agcgtcatcc agtgctttcc ggatgctctg aaccacatat tccttgaact
     6601 cctgttcatt accactcaag tcccagatgc gatccagatc cacccagcgc gtttccttgc
     6661 cgtccttcca atccttgaga agcaaagatt tggttgtcaa ttcatacttg ccaacggcgt
     6721 ccttgtcggc gggaatggcg cggaaagcca acttgccttc cttaagctga atcggaaaat
     6781 tcttttcgat cgcctccttc gtccatgtct ccatacggat gcaggttggg catctcgccc
     6841 catattcgta ataaaccgcc tctaccccgg atttcatcgt cacagttgtg gcggctggtt
     6901 cttcacggtt ttcagctccc tggtggttga taactttgac gatggcaagc aggatgagca
     6961 gggcaaccac ccagactaca gcctttttcc atccggagca tcctttggat gcctcactgc
     7021 tgcaacagct ccctccgcaa catgatacct ctttcgcatg agaatcattt tcttgaggta
     7081 acgcagattc tgctacgccg gaacaacagc aggatttctg ccgggaagaa tcagcctgaa
     7141 tggtctcttg aagaatcttc ttgatttcat ccttggatgg aactctcccc gaaacaaata
     7201 ccttgccatc cacagccaac gccggggtca cgagtacccc cgctacggca aattgcatgg
     7261 catccgtcac tttttccaga gaagcgtcca aatggagttc ctgcatggct tgcttcgtca
     7321 catccgccag tgcagtgcac ttggcgcatc ccggtccgta aacgacaatg ttgttcatgg
     7381 tattattgat ttagattttg aagaatatca aagtgtttgt gtagaaatca gaagaaacta
     7441 gagtatcatg tccttatctc cgcagaatgg gactttcctt caaacagtct aatacctggc
     7501 gaaggcacgg acaacacagg gaataataca cattcttccc ccgctgttca caagagagca
     7561 cacgattgtt cttcaacaca ttcaagtggc tggaaatcgt ggacatatcg cgcccggaat
     7621 ctttttgcag ttcgcagacg cagcgttccc catccagaag catctccacg atcatcaaac
     7681 ggacgggatg ccccaatgcc ttgaatacac cggcaagagg tatcagatcc ttgacggagg
     7741 aagccgggag cgaagaatgt gtacaggagc aatcaggcat aaacgatgga ttcattattt
     7801 tgaaaaatat aaaaatgtaa atctattttt cgttacgtcc atggcgcgcc gcgcaaacac
     7861 ggtatcctac ggcaacagcc ttgaaaacta tccccatgct ggacaggtca attcccttcc
     7921 gatatgctcg cttgcatatt ttcccgccac cgccatgctg cacatcggat gccatctttc
     7981 ctccgcaaaa ggctatgaag ccacgggccg ggttgccctc tccatcagcg ccaatacttt
     8041 tcagttcttc acccgnnnnn nnnnngggga gcaaagccaa agctattgac gaacaggata
     8101 ttgcccgttt tctggaatta gcgcggaaca acggattcgg aacgctgctg gcacacgccc
     8161 cctacacgct gaacccctgt tccgcagacc ccggcgtcgc ccgttttgcg gcccaggtgc
     8221 tgaaagagga tctggaactc atggaacatc tgccgggaaa tctttataac ttccatcccg
     8281 gctgccatgt aggtcaggga gcggaaaaag gcattgaact ggtggcgggc caattaaacg
     8341 gcgtgctttc tccggaacaa acgaccattg ttctgctgga aaccatgtcc ggcaagggaa
     8401 gcgaagtcgg ccggaccttt gaagaactgg ccaccattat ggagcgggtg gacctgaagg
     8461 acaagctggg cgtatgcctg gatacctgcc atgtttattc cgccggatat gatattgtca
     8521 acgggctgga ttccgtactg gaacatttcg acgccgtgct gggcctggaa cgcctgcgcg
     8581 ccatccacct gaacgacagc atgacgccct tctcctcctt caaggaccgc catgaaacca
     8641 tcggcaaggg ttccctggga gaacaggcct tcatcaacat catcaaccat cccgttctgc
     8701 gggaattgcc cttcttcctg gaaacgccca gggacgacgc cgggcacggc gaagaaataa
     8761 cgtggctgaa ggaacattac cgcaactgaa ccgccgccaa ggggcaccca tactgattga
     8821 actccggtct ttctcccgga gaaaaagctt gcatccgctt cggatacagg atacacaagc
     8881 ctcataagtt agtttgagct aatttcaata tctggtttcc aaggaagaac ccatccgatt
     8941 tttatgaaag aaatgcgcat cgcgaaaata acagggcgtg aaattctgga ttcacgcggc
     9001 aaccccacca ttgaggcgga cgtttatctg gaaggaggcg ccgtaggcac ggcgggcgtc
     9061 cccagcggag cttccaccgg ggaacacgaa gcctgggaac tcagggacgg cgacgcaacc
     9121 cgctaccagg gcaagggagt caccaaggcc gtagacaaca tccg
//
LOCUS       DAOMSU010000063         9164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_513_length_9164_cov_2.614777, whole genome shotgun sequence.
ACCESSION   DAOMSU010000063 DAOMSU010000000
VERSION     DAOMSU010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9164
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_513_length_9164_cov_2.614777"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PK077_04225"
     CDS             <1..185
                     /locus_tag="PK077_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23536.1"
                     /translation="NPRFMKGNRVVSELPKDRQDKNGREFTLRLSGSEKPDLVFDCQG
                     HGWFDADCDIIVTKES"
     gene            complement(417..1796)
                     /locus_tag="PK077_04230"
     CDS             complement(417..1796)
                     /locus_tag="PK077_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRN23537.1"
                     /translation="MDLFSLQEAEAREGFEPGADTSAMPLAARMRPRALDEVAGQKHL
                     LASGKLLRRAIETDRFTSLIFYGPPGCGKTTLAAVIARTTNAHFMMLNGVESNVADIR
                     EKIAQAQMRMSMHGRKTVLFVDELHRFNKAQQDVLLPHLEKGTVRFIGATTENPYFAI
                     NSPLLSRSQVFPLEPVPEEELAALLKRALADEVRGLGASRVDMEAEALNHLAAKADGD
                     ARKALTALEVAVLSTPAGKDGIIHVDISVAEESIQRKAIRYDRLGDSHYDTISAFIKS
                     MRGSDPDAALYWLGMMLEAGEDIRFIGRRLVIAASEDVGLADSNALRVALDAARAAEM
                     VGMPEARIPLAHAAVYLATAPKSNSAYVGINAAMDDVRNGKTLAVPEHLRTPTRKKLA
                     AAGGADAARLEYLYSHDYPEHYVPQAYLPEGRVYYTPTGNGLELRIRERMEYRRQLAE
                     NAGEGGKKG"
     gene            complement(1904..2299)
                     /locus_tag="PK077_04235"
     CDS             complement(1904..2299)
                     /locus_tag="PK077_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23538.1"
                     /translation="MRFFRDSSAKQWPAFKDPCFELALARRKEEARWGWIPFAVFVFF
                     LFVYRFFGLVMPSNNADGSQSWLFLLVLAGWGLLALVHMVVIPIWMLIASLHYWEVNR
                     RGLAVAGLLFSILAPVSMVFMLSCWSMFG"
     gene            complement(2664..>2859)
                     /locus_tag="PK077_04240"
     CDS             complement(2664..>2859)
                     /locus_tag="PK077_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRN23539.1"
                     /translation="ELAQNIIKGAGSSSKVLHAPERPGDVKHSRACADKLRNAGWQPR
                     HTLPEGLATTLEYFKGILGR"
     assembly_gap    2860..2890
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2891..3618)
                     /locus_tag="PK077_04245"
     CDS             complement(<2891..3618)
                     /locus_tag="PK077_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRN23540.1"
                     /translation="MKILVTGGAGFIGSHIVEHYQDKAEEIRVLDNLRTGYLRNLEGL
                     RHTFIEGSICDRELVRQAVQGVDYIFHMAALVSVPESMSKISECIDINVNGLLNVLEE
                     ASAAGVKKIVLASSAAIYGDNPTVPKLETMYPEPKSPYAITKLDGEYYLNMFRAEGKI
                     NTAAVRFFNVFGPRQDPKGAYAAAVPIFIEKAVKGEDITVYGDGSQTRDFIYVKDIVG
                     ALTFVAEHPEVIGVFNAGYGGQIT"
     gene            complement(3645..4739)
                     /locus_tag="PK077_04250"
     CDS             complement(3645..4739)
                     /locus_tag="PK077_04250"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRN23541.1"
                     /translation="MHDLKAVSTLFDLRGDFVHGYSYGSGHINDTYCIWVDQAGQRIR
                     YILQRVNHNVFKQPIPLMENVKRVTDHALKRLKEEQCPEAYRRTLTLVPSVDGKPYAL
                     DSDGNCWRVYPFIERARTYDQIETTRQCQEAARAFGEFQKLTADLPGEPLFETIPNFH
                     NTTSRLAALKEAIQSDPLGRVKEVQKEIDWYLSRESDCHLVVDYLKSGELPLRCTHND
                     TKLNNVMLDDVTGEGICVIDLDTTMPGSAIYDFGDMIRTATSPAAEDEKDVSKVTMRM
                     FMFEALVQGYLSSAKSFLTPLEKSLLPFSGKLLTMECGIRFLTDYLSGDVYFKIHRPE
                     HNLDRCRTQMALVESIEAQMDEMKAIVDRY"
     gene            complement(4752..5663)
                     /locus_tag="PK077_04255"
     CDS             complement(4752..5663)
                     /locus_tag="PK077_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009964936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRN23542.1"
                     /translation="MMKPTLLVLAAGMGSRYGGLKQLDPMGPNGEVVLDYSVYDAIRA
                     GFGKVVFIIRRDFEDIFKEKVGSRFAGKIEVDYAFQSLDDLPEGFSVPEGREKPWGTA
                     HALRAARHVVKEPFAVVNADDFYGADAFVQAAKFLTSTTDEPGKAHYGMIGYPLKNTL
                     SDNGDVNRGICSIKGGLLDGVEEFVKIQADEDGVVRGNNLKGERLPVDPAELVSMNFW
                     LFSPSFIELTEDHFIDFLKEHGQEPKSECFIPTVVDALIHADRADCAVLPTSSSWFGM
                     TYPGDKPMVVEGINKLIQQGVYPEKLN"
     gene            complement(5736..6455)
                     /locus_tag="PK077_04260"
     CDS             complement(5736..6455)
                     /locus_tag="PK077_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23543.1"
                     /translation="MRLFSIILCCAVQAVLGYDGPPPLTEVDGRSPIRMGGEMLGGYR
                     SYYVHRGEKMGADSTEGQISGGASLSDSWAVSGELFSIRNWQGRHFSQATLHGEVQYY
                     LADECTAGLFVNGQWYDSCPLKNGAEPGLSLKWNPTPSWSFRGSFLYDSGQEGAYSQW
                     GVTWQPLLCESVAMVNTVSLGFAHDYLGRNGLKELMVRTGALWAVSGGLRVEPFLGWY
                     CGYGRDDFKKVVLGLWCSYVF"
     gene            6559..7251
                     /locus_tag="PK077_04265"
     CDS             6559..7251
                     /locus_tag="PK077_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23544.1"
                     /translation="MVHFTLFGIPVYIRPSFWVVLAIFGGALSISSVEDLIYPALFVI
                     AGFVAILSHEMGHALVGRKLGGGQQTIVLELFGGLTSSHGMQLTRGGRALMILAGPMM
                     TLLLGIISLWLTWNIVAPVMTSYNLNFWDLAISPFTAALISPKLYILSCLIMIGEWWT
                     ILNLLPIYPLDGGQLIAQYIRSPRKVFMTGFITAILIGLLSFQLFHGYFIPIFMALFA
                     YSNYREYKNAPF"
     gene            7309..8829
                     /gene="proS"
                     /locus_tag="PK077_04270"
     CDS             7309..8829
                     /gene="proS"
                     /locus_tag="PK077_04270"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRN23545.1"
                     /translation="MSQQTAITPTRAQDFPEWYQQVIKAADMAENSEVRGCMVIKPWG
                     YAIWELIQKDLDQRFKDTGHTNAYFPLLIPISYLEKEAEHAEGFATECAVVTHHRLEA
                     QKDEATGKTRMIPTGELTEPFVIRPTSETVIGAAFARWTSSYRDLPLKVNQWCNVMRW
                     EMRPRIFLRTAEFLWQEGHTAHETREEAIEETLTMHKVYEEFQRDVLAIPTIPGEKTE
                     AERFPGAEQTYTVEAMVQDRKAIQAGTSHFLGQNFSKSQNICFAGRDNTQQFAWTSSW
                     GVSTRMIGALIMMHSDDDGLVCPPRVAPQQIVIIPVTPKEESRQAVLDHCEELARTLR
                     AKTFHGQPLRVLVDRRDLGGGAKKWEWVKKGVPVRLEIGPRDLEKGSVCLQRRDRPAN
                     EKSFVPETELLDTAADILQSIQDTLLQRAIAFRDSHIRPASTLRELEENFSGEGDADW
                     LQVPWDGSPEEEEELAKRLRISIRCIPLGELGRGEPAPCILTGRMTKRRVLWARSY"
BASE COUNT         2021 a         2817 c         2448 g         1847 t
ORIGIN      
        1 gcaacccccg cttcatgaag ggcaaccgcg tagtcagcga actgcccaag gaccgtcagg
       61 acaaaaacgg gcgcgaattc accctgcgcc tctccggttc ggaaaagccg gacctagttt
      121 tcgactgcca gggacatgga tggtttgatg cggactgcga catcatcgtg accaaggaat
      181 cctgattctt gccggcaaac aaacacgccg ccatatcaca accttcacag aggcaggata
      241 ttcccaaggg atttcctgcc ttttttccgg agggggaagc attcccaacc ggcgctcaag
      301 cggaagccgc ccctctctcc ctgccctttg ccggcccagg ccgaacaaca ttcccggcag
      361 cttcaaggga gcgataaggg aaacattctt ctcattcccg cgtcttcaag ggaagattac
      421 cccttcttcc cgccttcccc ggcgttctcc gccagttgac ggcggtattc catgcgttcc
      481 cggatcctca gttccagccc gtttccggtg ggggtgtaat acacgcggcc ttccgggagg
      541 taggcctggg gtacgtaatg ttccggatag tcgtgggaat acaggtattc cagcctggcg
      601 gcatccgcgc ctccggcggc ggccagcttc ttgcgcgtgg gcgtccggag gtgctccggc
      661 acggccagcg ttttcccatt gcgcacgtca tccatggcgg cattgatgcc cacgtaggcg
      721 gaattgctct tgggggccgt cgccaggtaa accgcagcat gcgccagcgg gatgcgggct
      781 tccggcatgc cgaccatttc cgcggcgcgc gccgcgtcca gagccacgcg cagggcgttg
      841 gagtccgcca gccctacatc ctccgacgcg gcgatgacca gccttcttcc gataaagcgg
      901 atatcctccc ctgcttccag catcatcccc agccaataca gggcggcgtc cgggtcagag
      961 ccgcgcatgg atttgatgaa agcggaaatc gtatcgtaat gggaatcccc caggcggtcg
     1021 tacctgatgg ccttgcgctg gatggattcc tccgcgacgg agatgtccac atgaatgatg
     1081 ccgtctttcc ccgcgggagt ggacaggaca gccacttcca gagccgtgag cgccttcctg
     1141 gcgtctccgt ccgctttggc ggccaaatgg ttcagggctt ccgcctccat atccacgcgg
     1201 gatgcgccca gcccgcgcac ctcatccgcc agggcgcgct tcagcagggc ggccaattcc
     1261 tcctccggca ccggttccag cggaaagacc tgggagcggg acagcagggg ggagttaatc
     1321 gcaaaatagg ggttttccgt ggtcgctcca atgaacctga ccgttccctt ttccagatga
     1381 ggaagcaaaa catcctgctg ggccttgttg aaccggtgca gttcatccac gaagaggacg
     1441 gttttccgcc cgtgcatgct catcctcatc tgggcctggg ctattttttc ccggatatcc
     1501 gctacattgg attccacgcc gttgagcatc atgaaatggg cattcgtcgt ccgggcgatg
     1561 acggcggcca gagtagtctt cccgcaaccg ggaggcccgt agaaaatcag ggacgtgaac
     1621 cggtccgtct caatggcccg tctcaacagc ttgcccgaag ccagcagatg cttctgcccg
     1681 gccacttcat ccagggcgcg cggacgcatg cgcgcggcca gcggcatggc ggaagtatcc
     1741 gctccgggct caaaaccttc ccgcgcttcc gcttcctgta aactgaacaa atccatcgga
     1801 aacactttac ccggttgaaa ggccggagaa aaggccaatc cccggaatcc tgaaagtttg
     1861 gcggaagcga caccgttccc ccctcaggac ggaaaatgca cctttatcca aacatggacc
     1921 agcaggacag catgaaaacc atgctgacag gagccaaaat gctgaacagc agccccgcaa
     1981 cagccaatcc cctgcggttc acctcccagt aatgaaggga ggcgatcagc atccagatgg
     2041 gaatcacaac catgtggacg agagccagaa gcccccagcc tgccagcacc agcaaaaaga
     2101 gccatgactg cgagccgtcc gcattattgc tcggcatgac aaggccaaag aagcggtaca
     2161 caaacaaaaa gaagacgaat acggcaaaag gtatccatcc ccagcgggct tcctccttcc
     2221 ggcgggccaa ggccaattcg aaacagggat ctttaaacgc aggccattgc ttggcggaag
     2281 aatctctgaa gaaacgcata tcgcccgaag tctctattat cgcgcctttc ttccttcaat
     2341 gatattcaat gccagtccgg ctttttcccg cagaaaattt tttcctctaa caaatacggg
     2401 acatcggctt cagccaatta ccttattgtc aaaccaaagg actgtgaaaa ttcatgaaaa
     2461 acttttgacc ataaatcatt ttttcattct tcctcccccc gctcttcttc ttttcccatg
     2521 atgggaaaag tctttttgca tgatccaaat ttcaacaccc gtccaatttg tcatacacat
     2581 ccccaatttc ccataaaaaa gactgcctcc gccacaggag cggagacagc ccggagtaat
     2641 ataaaaccgg aaattacgga aggctacctg cccagaattc ccttgaagta ttccagcgtc
     2701 gtcgccaggc cttccggcaa agtatgcctg ggctgccatc cggcattgcg gagcttgtcc
     2761 gcacaggcgc gggaatgctt gacgtctccc ggacgttccg gcgcatgaag caccttggag
     2821 gaagatcccg cacccttgat gatgttctgc gccagctctn nnnnnnnnnn nnnnnnnnnn
     2881 nnnnnnnnnn atggtgatct ggccgccgta accggcattg aacacgccga tgacttccgg
     2941 gtgttccgcc acaaaggtca gggctcctac aatgtctttc acatagatga aatcgcgcgt
     3001 ctgggagccg tccccataca cggtgatgtc ttcccccttg acagcttttt caatgaaaat
     3061 gggcacggcc gcggcatagg cgcccttggg gtcctgccgg gggccgaaga cattgaagaa
     3121 gcgcacggcc gccgtattga tttttccttc cgcccggaac atgttgaggt agtattcccc
     3181 gtccagcttg gtaatggcat aggggctctt gggttccggg tacatggttt ccagtttggg
     3241 caccgtagga ttatctccat aaatggcggc ggaagacgcc agcacgattt ttttgactcc
     3301 ggcggcggaa gcttcctcca gcacgttcag caaaccgttg acgttgatgt cgatgcattc
     3361 gctgatcttg ctcatggatt ccggcacgga gacgagcgcg gccatatgga aaatatagtc
     3421 cactccctgc accgcctggc gcaccagctc ccggtcgcag atggaacctt cgatgaacgt
     3481 gtgcctgagc ccttccaggt tcctgagata gcccgtgcgc aggttgtcca gcacacggat
     3541 ttcctccgcc ttatcctgat agtgttccac aatgtgggaa ccgatgaatc cggcgccgcc
     3601 ggttacgaga atcttcatgt ttttccgatg ttggtagata ttggttaata gcggtccaca
     3661 atggccttca tttcatccat ctgcgcctca atggattcta ccagggccat ctgcgtgcgg
     3721 cagcggtcca ggttgtgttc cggacggtgg attttaaaat acacgtcacc ggacaggtag
     3781 tccgtcagga agcggatgcc gcattccatc gtgagcaatt tgccggagaa cggcagcagg
     3841 ctcttttcca gaggcgtcag gaaactcttg gcggaactca ggtaaccctg aaccagagct
     3901 tcaaacatga acatgcgcat cgtgaccttg gagacgtcct tttcatcttc cgcggcggga
     3961 gacgtggccg tccggatcat gtcgccgaag tcgtaaatgg cggaaccggg catcgtggta
     4021 tccaggtcaa tcacgcaaat gccttctccg gttacatcgt ccagcatcac attgttcagc
     4081 ttggtatcat tgtgcgtaca gcgcaggggc aattccccgg atttcaggta atccaccacc
     4141 agatggcagt ccgactcacg ggaaaggtac cagtctattt ccttctgcac ttccttcacg
     4201 cgccccaggg ggtcggactg gatggcctct ttcagagccg ccaggcggga agtggtattg
     4261 tggaaattgg gaatggtttc aaacaggggc tctccgggca ggtctgccgt aagcttctgg
     4321 aattcgccga aggcgcgcgc cgcttcctgg cactgcctgg tagtttcaat ctggtcatac
     4381 gtgcgtgcgc gttcaatgaa cgggtacacg cgccagcagt tgccgtccga atccagggcg
     4441 tacggcttgc cgtccacgga gggaaccagc gtgagagtgc ggcggtaagc ttccgggcac
     4501 tgttcctcct tcaggcgctt cagggcgtgg tccgtcacgc gctttacgtt ctccatcaga
     4561 gggatgggct gcttgaacac gttatggttc acgcgctgga ggatgtagcg gatgcgctgt
     4621 cccgcctgat ccacccagat gcagtaggtg tcattgatgt ggccgctgcc gtaggaataa
     4681 ccgtggacaa aatcgccgcg gagatcgaac agggttgata cagccttgag atcgtgcata
     4741 gggaaaaagg cttagttgag cttttcaggg tatacgccct gctggataag tttgttgatg
     4801 ccttccacaa ccatgggctt gtcccccgga taggtcatgc cgaaccagga ggaagaagtg
     4861 ggaagaacgg cgcaatccgc ccggtccgca tgaatcaggg cgtccaccac ggtgggaata
     4921 aagcattcgc tcttgggttc ctggccgtgt tctttcagga agtcaatgaa atgatcctcc
     4981 gtcagttcaa tgaaggaggg ggagaacagc cagaaattca tggaaaccag ttctgcagga
     5041 tccaccggca ggcgttcgcc cttcaggttg ttgccgcgca ctacgccgtc ttcgtcagcc
     5101 tggattttca cgaattcctc cacgccatcc agaaggccgc ccttgatgga gcaaatgccg
     5161 cggttcacgt ccccgttgtc ggagagggtg ttcttcagcg gatagccgat catgccgtaa
     5221 tgggccttgc cgggttcatc cgtagtggag gtcagaaact tggcggcctg cacaaaagcg
     5281 tccgcaccgt aaaagtcatc cgcattcact acggcaaagg gttccttcac cacgtggcgg
     5341 gcggcacgca gggcgtgggc ggtgccccag ggcttttccc ggccttccgg cacggagaag
     5401 ccttccggca ggtcgtccag ggactggaaa gcgtaatcca cctcaatctt gccggcaaaa
     5461 cggctgccga ccttttcctt gaaaatatct tcaaagtcac ggcggataat gaataccact
     5521 tttccgaaac cggcccggat ggcatcataa acagagtagt caagaactac ttccccgttg
     5581 gggcccatcg ggtcaagttg cttgagaccg ccgtaacggc tgcccatgcc agctgctaaa
     5641 acaagaagtg taggcttcat catacgcagg ccattatgac caagcctccc cgtttggcaa
     5701 caggaaaatg ccggaaaacc ggagaaatcc gcccgttaaa aaacgtaaga gcaccaaagc
     5761 cccagcacta cttttttgaa atcgtcccgc ccgtagccgc aataccaccc caggaaaggc
     5821 tccacccgca gaccgccgga aacggcccac agtgcccccg tgcgcaccat cagctctttc
     5881 aagccgttcc tccccagata atcgtgggca aaacccaggg aaaccgtatt caccatggcg
     5941 acggattcgc acagcagggg ctgccaggta acgccccact gggaatatgc accctcctgt
     6001 ccggaatcat acaggaagga accgcggaaa gaccaggaag gggtgggatt ccatttaagg
     6061 gacaggcccg gctccgcccc gttcttcagg ggacaggaat cgtaccactg tccgttgaca
     6121 aaaagcccgg ccgtgcattc gtccgccaga taatactgga cttccccgtg taaagtggcc
     6181 tgactgaaat ggcggccctg ccagttgcgg atggagaaaa gttccccgct caccgcccag
     6241 gaatcagaca gggaggctcc gccggaaatc tggccctccg tggaatcagc ccccatcttt
     6301 tccccccggt ggacatagta tgaacggtag ccgccaagca tctccccgcc catgcggatg
     6361 ggggaacggc cgtccacctc cgtcaggggc ggcggcccgt cataccccag gacggcctgc
     6421 acggcacagc acaggatgat ggaaaacagc ctcatgaaag acttttaaca caattcgctg
     6481 cgccgctcca acgccaaaac ggtgcatgca cattctccgg gcttggcaaa acgggaatct
     6541 gcccttaccc tctcgcgcat ggttcatttc accctgttcg ggatacctgt ttacatccgc
     6601 cccagcttct gggtggtgct ggccattttc ggcggcgccc tcagcatcag cagtgtggaa
     6661 gatctcattt atccggccct gttcgtcatt gccggcttcg tcgccatcct gtcccatgaa
     6721 atggggcacg cgctggtggg ccgcaagctg ggcggcggcc agcagacgat tgttctggaa
     6781 ctcttcggcg gcctgacaag cagccatggc atgcaactga cgcgcggagg gcgggccctc
     6841 atgattctgg caggccccat gatgaccctg ctgctgggca tcatcagcct gtggctcacc
     6901 tggaacatcg tcgctcctgt catgacctct tacaacctga acttctggga cctggccatc
     6961 agccccttca cagcagcgct catctccccc aagctgtaca ttctctcctg cctcatcatg
     7021 attggagaat ggtggactat cctgaatctg ctgcccatct accccctgga cggcggccag
     7081 ctgatcgcac agtacatccg ctcccccagg aaagtattca tgaccggctt catcacggcc
     7141 atcctcatcg gactgctcag tttccagctc ttccacggct actttatccc catcttcatg
     7201 gccctcttcg cctacagcaa ttaccgggaa tacaaaaacg ccccctttta atattattcc
     7261 gagtttcctt cgcgggccct gcccgttcta ttcaacaaac catccaatat gtctcagcaa
     7321 accgcaatca cccctacccg cgcccaggat ttccccgagt ggtaccagca agtcatcaaa
     7381 gccgccgaca tggcggaaaa ttccgaggtt cgcggctgca tggtcatcaa gccatggggc
     7441 tacgccattt gggaactcat tcaaaaggac ctggaccagc gcttcaagga caccggccat
     7501 acgaacgcct atttccccct cctgattcct atctcctatc tggaaaagga agcggagcat
     7561 gccgaaggct tcgccacgga atgtgccgta gtcacccacc acaggctgga agcacaaaag
     7621 gatgaagcca ccggcaagac acgcatgatt cccaccgggg agcttacgga acccttcgtc
     7681 atccgtccca cctcggaaac cgtcatcggc gcggctttcg cccgctggac ctccagttac
     7741 cgggacctgc ccctcaaagt caaccagtgg tgcaacgtga tgcgctggga aatgaggccc
     7801 cgaatcttcc tgcgcacggc ggaattcctg tggcaggaag ggcatacggc ccatgaaacc
     7861 cgcgaggaag ccattgagga aaccctcacc atgcacaagg tttatgaaga attccagcgg
     7921 gacgtgctcg ccatccccac cattccaggg gaaaagacgg aggcggaacg cttccccggc
     7981 gcggaacaaa cctacacggt ggaagccatg gtgcaggacc gcaaggccat ccaggcaggg
     8041 acctcccact tcctggggca gaatttctcc aagtcccaga acatctgctt cgccggaagg
     8101 gacaacaccc agcaattcgc atggacaagc tcctggggcg tttccacccg catgatcgga
     8161 gcgctcatca tgatgcactc cgacgacgac ggactcgtct gcccgccccg cgtcgctccc
     8221 cagcaaatcg tcatcattcc cgtcacgccc aaggaagaaa gccgccaggc cgttctggac
     8281 cactgcgaag aactggcgcg caccctccgc gccaaaacct tccacggcca gccgctccgc
     8341 gtgctggtgg acaggcgcga cctgggcggc ggcgccaaaa aatgggaatg ggtgaaaaaa
     8401 ggcgtgcccg tgcgtctgga aataggcccc cgggacctgg aaaaaggctc cgtctgcctt
     8461 cagcggcgcg accggcccgc caatgaaaaa tccttcgtcc cggaaacgga actgctcgat
     8521 accgctgcgg atattctcca aagcatccag gacaccctgc ttcagcgggc cattgccttc
     8581 cgggactccc atatccgccc cgcctccact ctgcgggaat tggaagaaaa cttctccgga
     8641 gagggagatg cagactggct ccaggtgccg tgggacgggt ctccggaaga agaagaagaa
     8701 ctggctaaac ggctgcgcat ttccatccgc tgcatcccgc tcggcgagtt gggccgcggc
     8761 gagccggcac cctgtatcct caccggacgc atgacgaaac gccgtgttct ctgggccaga
     8821 agctactgaa ccctgacacc ccctttcaac gggattgttc caagacaggc aaaacgggcc
     8881 ttttcggaac aatcccgttt tttacgcctc tccactttcc ccaaaaacgg catgaacaag
     8941 aaacggagcg gcatacccca cagggcagaa ttctcccctc tcctgcccca tacgccggca
     9001 acaacgcaca catgtcggca aaacaggatt tttctctaaa aaaatcttgc caaggcagaa
     9061 aaaccttata tactcttggc acacggacgc cgtaaggctc caatcgttcc aagcggatgt
     9121 agctcagggg tagagcgcaa ccttgccaag gttgatgtcg tggg
//
LOCUS       DAOMSU010000064         9145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_517_length_9145_cov_3.253135, whole genome shotgun sequence.
ACCESSION   DAOMSU010000064 DAOMSU010000000
VERSION     DAOMSU010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_517_length_9145_cov_3.253135"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            373..1140
                     /gene="hisF"
                     /locus_tag="PK077_04275"
     CDS             373..1140
                     /gene="hisF"
                     /locus_tag="PK077_04275"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HRN23546.1"
                     /translation="MLAKRIIPCLDVTDGRVVKGTNFINLRDAGDPVECARAYDAQQA
                     DELVFLDITASSDGRATMADVVRRTAACCFMPLTVGGGIRSVKDMREMLLAGADKVSL
                     NTAAINRPELINEGAAAFGSQCIVVAIDAKRQASGRWGVSTHGGRKFVGLDAVEWAVE
                     AERRGAGEILLTSMDADGAKTGYDIELTRAVSSAVRIPVIASGGAGNLDHMVDVLVEG
                     KADAVLAASIFHFGEYTVPEAKAYFASRGIPVRPLAE"
     gene            1194..3326
                     /locus_tag="PK077_04280"
     CDS             1194..3326
                     /locus_tag="PK077_04280"
                     /inference="COORDINATES: protein motif:HMM:NF039643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6067 family protein"
                     /protein_id="HRN23547.1"
                     /translation="MRCLFFSLCSVIALTSFVQAQSLERHPLYEPALVSAGTPGDAGN
                     LFAGAKVTASGHYGSDRPELAVDGQTNNAGKYWGCEGIPVWLQIDMGKPRTLSALHVW
                     PYWEGGRIYKYKIEGSEDGKNWKMLADQSSNSIAATPEGVPFKFNPQTVRYVKITFLG
                     NSAGNEKGGHLVEIKGYGPDAALSLQAAAVKDYDRIPYNGAPGQEMLQDAVRLSGWRG
                     ERAGGQIAVWSSQAQPQLSASCAGVKNAAGQIIPVRTAMIRYTRGGNRLISDIIGSEN
                     SCDLQAGGVRPVWVEVNIPPSAKPGVYKGKVVVSAESGSPVSVPVILEVAPESLPAPA
                     HWQVHLDLWQHPQAVARWHDVEPWSPEHFALMKPVMKRLADAGQKAITCSLIDEAWNA
                     QTYDWFPPMIEWVKGKNGTMRWNYANFDKWVSFMMNEVGVKGQISCYTMIPWNMKIRY
                     LDEATGKYKFLALKPNDPSYEAIWGPFLTDFRKHVKSKGWLGKTCIGLDERPDAMVRA
                     AKNVLDKYAPEFKIVSAVNRPTAMTRDVYDVSPVIDHAGTVTGDLLAQRKKEGKKTTF
                     YVCVHPKKPNTFTISPLAEAEWLPLFAAANHLDGFLRWAYNSWNRNPFEKTDFGNWPA
                     GDCYLVYPGNLSSLRFEKLRDGLEEFEKVNILRARAAKNPKAKAAVARMDEELSKLFT
                     VEKSRGNSHEEDVRKAREIIRKTTEISR"
     gene            complement(3410..3730)
                     /locus_tag="PK077_04285"
     CDS             complement(3410..3730)
                     /locus_tag="PK077_04285"
                     /inference="COORDINATES: protein motif:HMM:NF013777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRN23548.1"
                     /translation="MTYYNSSCPVETTLQLISSRWKVLILRDLLEGTRRFGELRKSIG
                     PVTQKVLTANLRSMEKDGLLTRKVYAEVPPRVEYTLTELGRSLRPVLASMAEWGSKYQ
                     RENR"
     gene            3854..4291
                     /locus_tag="PK077_04290"
     CDS             3854..4291
                     /locus_tag="PK077_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRN23549.1"
                     /translation="MNKIIIANYRETEPQVISREGNSFAVKPVIREADAGKCAASFVE
                     VEPGRYAYGYHYHEVNEEVFYIIRGHAVVRTPEGEKNLKEGDAIAFPASPEGAHVIRN
                     GSATEKLVYLDVGTRLTPDVVHFPDTASGMVYSSSGVHHFRES"
     gene            complement(4758..>7618)
                     /gene="nifJ"
                     /locus_tag="PK077_04295"
     CDS             complement(4758..>7618)
                     /gene="nifJ"
                     /locus_tag="PK077_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRN23550.1"
                     /translation="EATPAIVQNAMDKFAELTGRSYHLVDYTGAPDAERVIILMGSGA
                     EAVEETVEAMMTRENAKVGVLKVRLFRPFPAAELVKALPATVRKIAVLDRTKEPGSQG
                     EPLHQDIIQALFDAQANGTLPFTNGMPKVVGGRYGLSSKEFTPAMVKGVYDNLALDTP
                     KNHFTIGINDDVLGTSLPYDEDYSTEADDVTRAMFFGLGSDGTVGANKDAIKIIGQHT
                     NLYVQGYFVYDSKKSGSSTISHLRFGPRPIKSTYLITKANFLALHQPSLLDLFDFLKN
                     AANGATFLMNSPHPADKLWDTLPARMQQQILDKNIKLYTIDAFAVARKTGMGGRINMI
                     MQTCFFKLASVIPADEAIGYIKKAIAKTYAKKGQEVVDKNIAAVDATLENLHQVDTTG
                     KTVNGHAIPPAMSPDAPDYVQNVLGKMMCGEGDSIPVSQMPVDGTFPNGTSQYEKRNL
                     ALDLPEWDPTLCIQCGKCTAVCPHAVIRSKFFSPDALANAPEGFECLDAKHPDWKGEK
                     FVIQISPNDCTGCTLCADVCPAKSKTDPTHKALTMVPAGKIHDKEEANWNFFLSLPDV
                     DRTKVRTDNIRSMQVLRPLFEFSGACAGCGETPYVKMLSQLFGNRLVVANATGCSSIY
                     GGNLPTTPWSHDSEGRGPAWANSLFEDNAEFGLGFRVSLDKQMEHAIELLHEAAGAVG
                     QELVDRILANPQKDEADIAQQRENIEELKKKIAGKPEYARLLTLADKLVRKSVWILGG
                     DGWAYDIGYGGLDHILASGRNVKVLVMDTEVYSNTGGQCSKSTPRAAVAKFATTGKPG
                     VKKDLGLMAMTYGNVYVASVALGAKDEHTLKAFVEAEAYDGPALIIAYSHCISHGINM
                     AKGLQQQKAWVDSGRVLLYRYNPDLARQGKNPLIVEGKGPKGDLRDVLLSENRFKLLA
                     KTNKEGFEKLLEEAQKDVWHRWNLYQSIAGMGSDKPAE"
     assembly_gap    7619..7628
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7629..8344)
                     /locus_tag="PK077_04300"
     CDS             complement(<7629..8344)
                     /locus_tag="PK077_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRN23551.1"
                     /translation="MSDQTTVTYSTIDANEAVASVAYRFSEVIAIYPITPSSPMGESA
                     ESWAAVNRKNLWGTVPSVVEMQSEGGAAGTVHGALQTGALATTFTASQGLLLMIPNMF
                     KIAGELTPAVFHVAARSLAYQALSIFGDHSDVMSARSCGWAMLCGSSTQEAADFAAIS
                     HAATLESRIPFINFFDGFRTSHEIGKIADITDDTLNGMIKQEWLDSFRERALTPDAPV
                     LRGTAQNPDVYFQGRETVNR"
     gene            8690..>9145
                     /locus_tag="PK077_04305"
     CDS             8690..>9145
                     /locus_tag="PK077_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HRN23552.1"
                     /translation="MSEQQQAHPTTTESELIAVRRDKLAKIRELGIDPYGARFDVTTT
                     PAGLKADFQEDKQVAVAGRLLAIRDMGKSQFFVIGDVRGKIQGFLHKNEVDETTWKLW
                     KLLDRGDWVGIRGTTFLTRTGEPTVKVSGLTILSKSLRPLPDKWHGLADK"
BASE COUNT         1954 a         2346 c         2752 g         2083 t
ORIGIN      
        1 caagccatct ttcatgcagt ttagcagaca ctagtcatac tgcaaatatt tttggctggc
       61 taaatatata gttcccctac ttttatttgt ctggttgaag cgcagcacga aactattgac
      121 gcaaaagccc attcaaggag attatcgctt tctccggcga taaggagtaa attggtcgtc
      181 aagtaaacaa agccgccgtt aacggcttga gcgacgttat accagaaaaa attttcttac
      241 tggcatatac gggcggcagt actgctgaaa agggcatcgg gtcaaaaaga agaggttttt
      301 tatcaaaagg catcccgcgg caagccgtat tttaggcttt tcctttggcg gcggtgatgg
      361 cttgataggg ctgtgctggc taaaagaatc attccgtgtc tggacgtaac ggacggcagg
      421 gtcgtcaagg ggaccaattt cattaatctg agagatgccg gggatcctgt agaatgtgcc
      481 agggcgtatg atgcccagca ggcggatgaa cttgtttttc tggatattac cgcttcttcc
      541 gatggccgtg ccaccatggc ggacgtggtg cgccgcacgg cggcatgctg cttcatgccg
      601 ctgacggtag gcggcggcat ccgttccgta aaggacatgc gcgaaatgct tctggcgggt
      661 gcggacaagg tttccctgaa tacggcggcc atcaataggc cggagctgat taatgaaggt
      721 gcggcggctt tcggcagcca gtgcatcgtc gtcgctattg acgccaagcg ccaggcttcc
      781 ggcaggtggg gcgtttccac ccatggaggc cggaagttcg tggggctgga tgccgtggaa
      841 tgggctgtgg aggcggaacg ccgcggagcc ggagaaatct tgttgaccag catggatgcg
      901 gacggagcca aaacgggtta tgatattgag ttgacccgcg ccgtaagcag tgcggtgcgc
      961 attccggtaa tcgccagcgg gggtgcgggt aatttggatc acatggtgga cgtgctggtg
     1021 gaagggaagg cggatgccgt gctggcggct tccattttcc atttcgggga atacacggtg
     1081 ccggaagcca aggcttactt tgcttccaga ggaatacccg tacgtccttt ggcggagtag
     1141 cggaaacagc ttttcttttg agagaaaatg aaattccggc gcgtaatatg cgtatgcgct
     1201 gtcttttttt ttctctatgt tccgttattg ccttaacgtc ttttgtccag gcccagtccc
     1261 tggaaaggca tcccctgtat gaacccgccc ttgtttccgc aggaactccg ggtgatgcag
     1321 gtaatttatt tgccggggcc aaagtaaccg cttccggtca ttacggcagt gaccgtccgg
     1381 aactggccgt ggacgggcag acgaataacg ccggcaaata ctggggttgc gagggcattc
     1441 ccgtctggct ccagatagat atgggaaagc cccggacgct ttccgcactg catgtatggc
     1501 cctattggga gggagggcgc atttacaaat ataaaataga aggttccgaa gacggaaaaa
     1561 actggaaaat gttggcggat cagtcttcca acagcattgc cgccactccg gaaggcgttc
     1621 ccttcaaatt caatccgcag acggtccgtt acgttaaaat cactttttta ggcaatagcg
     1681 ccggcaatga aaagggggga catctggtgg aaatcaaagg gtacggaccc gatgccgccc
     1741 tgagcctgca ggctgcagcg gtgaaggatt atgaccgtat tccttacaac ggcgctcccg
     1801 ggcaggaaat gcttcaggat gccgtgcgtc tgtccggctg gagaggcgaa cgcgccggcg
     1861 ggcagattgc cgtctggtct tcccaggcgc agccccagtt atcggcctcc tgcgccgggg
     1921 taaaaaacgc tgccggacag attattcctg tccggactgc catgatacgc tacaccaggg
     1981 ggggcaacag gctgatttca gatatcatcg gcagcgaaaa cagctgcgat ttgcaagccg
     2041 gaggcgtgcg tccggtatgg gtggaggtca atattccccc gtctgccaaa cccggcgtgt
     2101 acaaaggaaa agtggtagtt tccgcagaaa gcggttctcc cgtcagtgtg ccggtaattc
     2161 tggaggtggc gccggaatcc ctccctgctc ccgcgcattg gcaagttcat ctggacttgt
     2221 ggcagcatcc gcaggccgtg gcccgctggc atgatgtgga accgtggtct ccggagcatt
     2281 tcgcgctgat gaaaccggtg atgaaaaggc tggcggatgc cgggcaaaag gccattacat
     2341 gctccctgat tgatgaagcc tggaatgccc agacttatga ctggttcccg cccatgattg
     2401 aatgggtcaa gggcaaaaac ggaaccatgc gctggaatta cgccaatttt gacaagtggg
     2461 tttccttcat gatgaacgag gtgggcgtca aggggcaaat atcctgctat accatgattc
     2521 cctggaacat gaaaatccgt tatctggatg aggcgaccgg aaagtacaaa tttctggctc
     2581 ttaagccgaa cgatccctcc tatgaagcta tctgggggcc tttcctgacg gattttcgca
     2641 aacacgtcaa gagcaagggc tggctgggca aaacctgcat cgggctggat gaacggccgg
     2701 acgctatggt cagggcggcc aagaatgtgc tggacaagta tgccccggaa ttcaaaatcg
     2761 tttccgccgt caatcggcct acggccatga cccgggacgt ttatgacgtc tctcctgtaa
     2821 ttgaccatgc aggcacggtt acgggcgatc tgctggcgca gcgcaaaaag gagggaaaaa
     2881 agacgacgtt ctacgtctgt gtccatccca aaaagcccaa caccttcact atttccccgc
     2941 tggcggaggc ggaatggctt cccctctttg ccgccgccaa tcatttggac ggctttttga
     3001 gatgggctta taattcctgg aaccgcaatc cgtttgaaaa gacggatttc gggaactggc
     3061 ccgcgggaga ctgctacctt gtttatcccg gcaatctcag ttccctgcgg tttgaaaaac
     3121 tccgggacgg actggaggaa tttgaaaagg tcaatatcct gcgcgcccgc gccgcaaaaa
     3181 atcctaaggc gaaagctgcc gtagcccgca tggatgaaga gctttccaag ctctttaccg
     3241 tggaaaaaag ccgcgggaat tcccatgagg aagacgtgcg gaaagcccgt gaaattatcc
     3301 gtaaaacgac ggaaatttcc cgttgacctt tcttgcggga aaaagggaaa ccgctgtatt
     3361 tccatgtctt tccggaggtt cggttaagct ccggaaaggg aggggaggat cagcggtttt
     3421 ctctttggta tttcgatccc cattccgcca tggaggccag gacgggacgc agactgcgcc
     3481 ccagttccgt aagagtatat tccacgcgcg gaggtacttc cgcatacacc tttcgcgtaa
     3541 gcaggccgtc cttttccata gaacgcaggt tcgcggtcag aactttctgc gtcacaggcc
     3601 caatggattt gcggagttct ccaaaccgcc gggtgccttc gagcagatcg cggaggatga
     3661 gcactttcca gcgggaggaa atcagttgga gagtagtttc aacagggcat gatgaattgt
     3721 agtaagtcat ttagtttacg caatggtttc ttttaggaat tcaggttact attttctgcc
     3781 tactttacag aaagaatgtt gccgcctatt ttaataggta cttgccgtta cggcaagcag
     3841 aacagataat gccatgaaca agatcatcat tgccaattac agggagacgg agcctcaggt
     3901 tatcagccgg gagggcaatt cctttgcggt aaagcccgtc attcgggaag cggatgccgg
     3961 aaaatgtgcg gccagtttcg tggaagtgga gccggggcgg tacgcctatg gctaccatta
     4021 tcatgaagtg aatgaagagg tattttacat catcaggggc catgccgttg tgcgtacgcc
     4081 tgagggtgaa aagaatttaa aagaggggga tgcgattgca ttccccgcca gcccggaggg
     4141 agcgcatgtt atccgcaacg gatccgctac ggagaagctg gtctatctgg atgtgggcac
     4201 tcgcctgacg cctgatgtgg tgcacttccc ggatacggca tccggaatgg tatattcctc
     4261 ctccggtgtg caccattttc gggaatcttg aagcagcatc cctgttttaa agcctttttc
     4321 cggtttttcc accggcgcgg tccgcaggaa agcgggccgc gctcctatgg ggaggaaggt
     4381 gtgagaaaac agtattccct tggccgcatg agatgcccgt ctttaccttg gacatggctg
     4441 gcagcaaagg ctgcggttcc ttgaaggcct tcaaaacctc catgcggaaa ttaacccgta
     4501 taaggtgata tattaaaacc cggccttgca gcaaggtgcg cgcccttttt ttgtgctgcg
     4561 ggggaaggat gaattctccg gctttcagga agggaaagta ctggcgcggg aagcctgctt
     4621 gcgtggctgt atggcggggc ggaaaagcag tcccctcccc gaaatattca tggtttgttt
     4681 ttcatgaata tccggcagta aaaaacgccc ctgccggata agccggacag gggcgttgaa
     4741 attaagcggc ctgtacctta ttccgcaggt ttgtcggaac ccatgccggc aatgctttgg
     4801 tacaggttcc agcggtgcca gacatccttc tgggcttctt caaggagttt ttcgaatcct
     4861 tccttattgg tcttcgccag aagcttgaag cggttttcgc tgagcagcac atcgcgcaga
     4921 tcgcctttcg ggcctttgcc ttccacaatc agcggattct tgccctggcg ggccaggtcc
     4981 ggattatagc ggtaaagcag gactcggccg gaatccaccc atgccttctg ctgctgcagg
     5041 cccttggcca tattgatgcc gtgggagatg cagtgggagt aggcgatgat gagagccggg
     5101 ccgtcatagg cttccgcttc cacaaaggct ttcagcgtgt gttcgtcctt ggcgccgaga
     5161 gcgacggagg ccacgtacac gttgccgtaa gtcatggcca tgaggcccag atccttcttc
     5221 acgccgggct tgccggtcgt ggcgaatttg gctactgcgg cgcgcggggt ggatttggag
     5281 cactggccgc cggtgttgga gtatacttcc gtatccatga ccagcacctt cacgttccgg
     5341 ccgctggcca gaatgtggtc caggccgccg tagccgatgt cgtaggccca gccgtcgccg
     5401 cccagaatcc acacgctctt gcggacaagt ttatccgcaa gggtcaggag gcgcgcgtat
     5461 tccggcttgc cggcgatttt cttcttgagt tcttcaatgt tttcacgctg ctgggcgatg
     5521 tctgcttcat ccttctgcgg attagccagt atccgatcca ccagttcctg gcccacggca
     5581 ccggcagctt catgaagaag ctcaatggcg tgttccatct gcttgtccag ggagacgcgg
     5641 aagccaaggc cgaattcggc gttgtcttca aacagggagt tggcccaggc ggggccgcgc
     5701 ccttcagagt catgggacca cggggtggtg ggcaggttgc cgccgtaaat ggaggagcag
     5761 cccgtagcat tggcgaccac caggcggttg ccgaagagct gggagagcat ttttacatac
     5821 ggggtttcac cgcagccggc acaggcgccg gagaattcga acagcgggcg cagcacctgc
     5881 atggagcgga tgttgtccgt tcttaccttg gtgcggtcca cgtccggaag gctcaggaag
     5941 aaattccaat tggcttcttc cttgtcgtga atcttcccgg ccgggaccat ggtcagggcc
     6001 ttgtgcgtcg ggtccgtctt gctcttggcc gggcagacgt ccgcgcagag cgtacagccc
     6061 gtacagtcgt tcggggaaat ctggatgacg aatttctctc ccttccagtc cggatgcttg
     6121 gcatccaggc attcaaaccc ttccggagcg tttgcaagag cgtcaggaga gaagaatttg
     6181 ctgcggataa ccgcatgcgg gcagacggcg gtacacttgc cgcactggat gcagagggtc
     6241 ggatcccatt ccggcagatc cagggccaga ttgcgctttt cgtactggga cgttccgttc
     6301 gggaatgtgc cgtccacggg catttggctg accgggatgc tgtcgccttc accgcacatc
     6361 atcttgccaa ggacgttttg tacgtagtcc ggagcgtccg gggacatggc gggcgggatg
     6421 gcgtgtccgt ttacagtttt tccggttgtg tccacctggt gcaggttttc cagcgtggcg
     6481 tccacggcgg cgatgttttt gtccaccact tcctggccct tcttggcgta ggttttggca
     6541 atggccttct tgatgtagcc gatggcttca tccgcgggaa tcacgctggc cagcttgaag
     6601 aagcaggtct gcatgatcat attgatgcgg ccgcccatgc ccgttttgcg ggccacggca
     6661 aaagcgtcaa tcgtgtagag cttgatgttc ttgtccagaa tctgctgctg catgcgggcg
     6721 ggcagcgtgt cccagagctt gtccgccggg tgcgggctgt tcatcaggaa agtggcgccg
     6781 ttggcggcat tcttcaggaa gtcgaacaaa tccagcaggg aaggctggtg cagggccagg
     6841 aagtttgcct tggtgatcag gtatgtggat ttgatcggac ggggaccgaa gcgcaggtgg
     6901 gaaatggtgg aggaccccga tttcttggag tcatacacga agtagccctg cacatacagg
     6961 ttcgtatgct ggccgatgat cttgatggcg tccttattgg cgcctacggt gccgtcggaa
     7021 ccgaggccga agaacatggc ccgcgtcacg tcgtccgctt ccgtggaata atcttcatca
     7081 tacgggaggc tggtgcccag tacgtcgtcg ttgatgccga tggtgaagtg attcttggga
     7141 gtgtccagag ccaggttgtc atagacgccc ttgaccatgg cgggggtgaa ttccttggag
     7201 gagaggccgt agcgtccgcc taccaccttg ggcatgccgt tggtgaaggg cagcgtgccg
     7261 ttggcctggg cgtcgaagag ggcctggatg atgtcctggt ggagcggttc tccctgggag
     7321 ccgggttcct tggtgcggtc cagcacggcg atcttcctga ccgtagcggg gagggccttg
     7381 acgagttcgg cggcggggaa tgggcggaac aggcgcacct tcagcacgcc taccttggcg
     7441 ttttcccggg tcatcatggc ttctaccgtt tcttccacag cttcagcgcc ggatcccatc
     7501 aggataatga cgcgttcggc gtccggagcg ccggtgtaat ccaccagatg gtaggaacgg
     7561 ccggtaagtt ccgcgaactt gtccatggcg ttctggacaa tagccggagt ggcttcatnn
     7621 nnnnnnnnaa acggttgacc gtttcacgtc cctggaagta aacgtccggg ttctgggcag
     7681 tgccgcgcag aacgggagca tcaggagtca gggctcgttc ccggaaggaa tccagccatt
     7741 cctgcttaat catgccgttc agggtatcgt ccgtgatgtc tgcaatcttg ccgatttcgt
     7801 gggacgtgcg gaacccgtca aagaaattga tgaaaggaat gcgggattcc agtgtagcgg
     7861 cgtgggaaat agcggcgaag tccgcagctt cctgagtgga ggagccgcac agcatggccc
     7921 atccgcagga gcgggcgctc atcacgtcgc tgtggtcgcc aaaaatggaa agggcctggt
     7981 aagccaggga ccgggcggct acgtggaaca cggcgggagt cagttcccct gcgattttga
     8041 acatgttcgg gatcatcagc aggagcccct gggatgcggt gaacgtagtt gcaagcgctc
     8101 cggtctggag ggcgccgtga acggtgcctg ccgcgccgcc ttcactctgc atttccacta
     8161 cggagggaac ggtgccccac aggttcttcc tgttgacagc ggcccagctt tcagctgatt
     8221 cacccatcgg agaagatggt gtaatggggt aaatggcaat gacttcagaa aaacgatagg
     8281 ctacggaggc tacggcttcg ttagcgtcaa tcgtgctgta agttacggtt gtctgatcac
     8341 tcataagtct gcacggatag tatataattg ataaataagg tgcgtcttaa cgggaacatt
     8401 tataacatgt ctgttgccaa aggtaaaagc aattatgcgt atgtggggat ttgccggcca
     8461 ttttttaata ataagaccag ttttgtttca attcctttga atatcgtgct ttatgtggtt
     8521 ctgtccaggc tttttgcaaa acggccggaa tactggggca tgttccggcg tccggcttcc
     8581 gaaaaagggg gggaaggaac cttcgggtca aaaccggccg ccaaaaatgt cccctctgcc
     8641 tgacaatgcg ctagccaaaa cggaagcaag cctttagaat ccccgcacta tgtccgaaca
     8701 acagcaggca catccgacta cgacggaatc cgaacttatt gccgtgcgcc gcgacaagct
     8761 cgccaaaatc cgtgagctgg gaattgaccc ttacggcgca agatttgacg tgaccaccac
     8821 tcccgccgga ttgaaagccg atttccagga agacaaacag gtagccgtcg ccggccgcct
     8881 tctggctatt cgcgacatgg gcaaatccca gtttttcgtc attggggacg tacggggaaa
     8941 aatccagggg ttcctgcata aaaatgaggt ggatgaaacc acctggaagc tttggaagct
     9001 cctggaccgc ggggactggg tcggcatcag gggaaccacg ttcctgaccc gtaccggaga
     9061 acctaccgta aaggtatccg gactgaccat tctttccaaa agcctccgcc ccctgccgga
     9121 caagtggcac gggctggcgg acaag
//
LOCUS       DAOMSU010000065         9010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_535_length_9010_cov_2.562814, whole genome shotgun sequence.
ACCESSION   DAOMSU010000065 DAOMSU010000000
VERSION     DAOMSU010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9010
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_535_length_9010_cov_2.562814"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="PK077_04310"
     CDS             complement(<1..224)
                     /locus_tag="PK077_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRN23553.1"
                     /translation="MSSKLLEGKVGVVVGVANKRSIAFAIAKAWHEAGAKLIFNYQGE
                     RLKGGVLKLIHENFGEDVPVYDLDVSSDESV"
     gene            complement(292..1167)
                     /gene="folD"
                     /locus_tag="PK077_04315"
     CDS             complement(292..1167)
                     /gene="folD"
                     /locus_tag="PK077_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRN23554.1"
                     /translation="MQIIDGKQVASQVLEEVERDIARLREQGITPGLAVVLVGEDPAS
                     QVYVNSKVKKCAELGMNSRKIVLPSDASQEELLRIVRELNVDPAVHGILVQSPPPPHI
                     DEAAVVLEIDPAKDVDGFHPENVAKLVLEDETGFVPCTPLGCMRLLKAAGIETAGANA
                     VVIGRSMIVGKPMAHLLMSKQANATVTVAHSRTKNLPELCRSADIIVAAIGRPAFVTA
                     DFVKDGAVVVDVGINRVEDASAKRGYRIVGDVAYDEVAPKCRAITPVPGGVGPMTIAM
                     LMANTVKACRQQTGA"
     gene            complement(1140..1868)
                     /locus_tag="PK077_04320"
     CDS             complement(1140..1868)
                     /locus_tag="PK077_04320"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRN23555.1"
                     /translation="MLLLNPIVRKTSEKRSRWWTRLAGFGLWSVLQPICMSLNIRSIK
                     EEHDDVYTAPFLVALWHNRTMVPGYAWSLAQKPLKMSVLTSASKDGALVEAVCRHFGL
                     GAVRGSSGRRGALAYMEMMRKVQEGDVCFCFTPDGPRGPIYEVHHGIVKLASQSGLPI
                     VPVCIEYESCWRLNKTWDRYAIPKPCSNVNILWKKRLFIPPDATDEQVADYARLLAGL
                     LGEGLPDFPPLNQSLLCKSSMENK"
     gene            complement(1884..>2221)
                     /locus_tag="PK077_04325"
     CDS             complement(1884..>2221)
                     /locus_tag="PK077_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23556.1"
                     /translation="ISLFEQGFLGVTGGVYSNGTSLTGKLVFKIPSYMVDSRKLPSGV
                     TQEGHEIILPVKISGTPESPQDNSAGMFQEEAPRTGRPEMHTAPSRGIALPAPSNSSP
                     PRITVDGFM"
     assembly_gap    2222..2231
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2232..3462)
                     /locus_tag="PK077_04330"
     CDS             complement(<2232..3462)
                     /locus_tag="PK077_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23557.1"
                     /translation="MRQLHASDSQGVERKREVVVRPDGSKVIRVEKRRRTYSDDQRDA
                     KQAMLKNKRFLIGLCILVLLVAGALAGTYMYRLTSFNTEEFAGRLQADLSAAWGGEVK
                     ISGMAVDGLTMKASRIKVSFPEDSCLSFVAMEGISGEISAVPLFMGKIKGESLNVSRA
                     VVGLRPGFVKFAVPQTKRELPFVFRRYTAPRLEVGYAHGTEEFTLERNGAPFYMTAEA
                     YIRTSGNGPEPEYVLDLSQQKLKIKGWPLMGVDSGSVILNGSGVKQLTFSGFLDKGRL
                     LNKPTDLSPFMITGNFLLGTDFRHQAWTLTGKNFDLASLLGDDFSSLLKVQTGEQEGD
                     EKHELKLDFALPVTTEQKRPSLKGYSGSVIKATMIRLPVLRLLAAMAARNTEIRLPYS
                     SPVFTSGSFLLESDGTGR"
     gene            complement(3541..6042)
                     /locus_tag="PK077_04335"
     CDS             complement(3541..6042)
                     /locus_tag="PK077_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN23558.1"
                     /translation="MSAIQQIAMALAVAGIGAGQGLAQEGAGAARRAAARMEEQAQAS
                     MLLLGQARQQYSEGKYQEALDNYRRSLTALPKSPNMEKRRRFLETSIADASVAVAQEY
                     IKAGRYDEAVKLLEDALKSTPDHALARRTLEIARDPVRTNPALSPEHVKNVEEVNRLL
                     HLAFGYYELGQYDAALKEFTSVLKTDPYNTAARRGMELVNRARTAYFSAARDETRSSA
                     LADVSRQWEMPVPLTETPEEPASFSRPLDNPVVNVDQKLGMIRLPRVQLDGATVQEAV
                     DYLRSQARTHDATAMTAAERGVNISVDPGPADSVSARDAAAKRITLNLQNVPLREALE
                     YVARASGLILRTNAFGAELVSSSDGTSYMVTKSITLPPGFFSGLSEDSGAENSDPFSS
                     GDSGSSGMTLKRVDPQKALASMGVKFPEGSFIKYNSGNSTLLFHGTPRDLSMLEELVA
                     ARTAEQPLQVVVSATFLEVNQTDLEELGFNWIVNLNLDPTKWFMGGAGTDKNDYNNSV
                     LDSAANVAGAVAPAGVVGGLRSGNQVFTEDSIDSMIERGTSARSSDATYVPGGAPSIV
                     TLRGMWSHADITMIMRGLSQKKGTDIMQHPSVIVRPGEKATFFSGRELIYPTEYDPPE
                     VPNSTGNNNDWGDNDNNGGGIPVMPMTPAHPTAFETRQLGTIFNVEVTGISDDKSIVE
                     MTVVPEIVDFDGFINYGTSLFVPMVSQEKDAKEDVVMVKTSDNFILQPVFSTRRLTAP
                     VRIATGNTLVIGALKKSTSITYEDKIPVLGDIPWVGRLFRSKGSKEQRKAIIIMVKAE
                     VVDPGGKKLYTPDTSIPDEEVPAGDASLPALSNVQ"
     gene            complement(6178..7902)
                     /locus_tag="PK077_04340"
     CDS             complement(6178..7902)
                     /locus_tag="PK077_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRN23559.1"
                     /translation="METHGVDWGSRETNGCGTFNGVTDKALREIARMGFTHIWLTGVL
                     RHATQTSHPGLAAQPKSIVKGLAGSPYAVVDYFDVDPDLASSPEKRMDEFKALVKRCR
                     TVGLLPMMDFIPNHVSRAYLADWDGHDDFGEGDDPHTFFSPEQGYFYLTSNSPGDGPP
                     LRLPDGLFEGEMTFGRVTGNNAVTWNPGKYDWYETVKLNYGYNFMAGLPALRLLPGWT
                     SPKQHVPKTWRIMDDILSFWQSLGIGGFRCDMAQMIPMAFWKWAISRSRVRLPDVFFM
                     AEAYNDHMKTTPGDPCAALLDAGFNAVYDSDCYHLALHMYTENNWANDFDRLFRSNPI
                     YMTHGVRYVENHDETRVCSPLSWGGVGRVVLPAVMTLVYASGSGPVLVYNGQEVGERA
                     EGPGGFGGHDGRTSIFDYTCLPRLQPWVANGRFDSSLLPEESAELRNFHRKLLPLMQH
                     PALDRGDFYGLNWANMKNTTFGREPAEDTSGHWVYAMLRHDARARATVLVVGNLSPNI
                     NFYNLRISFPQHAFGWCGIQTDRVRVRNLMDAEGEDRIFERRELMDYGLSHPLKPGHV
                     GVLELSAV"
     gene            complement(7974..>9010)
                     /locus_tag="PK077_04345"
     CDS             complement(7974..>9010)
                     /locus_tag="PK077_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRN23560.1"
                     /translation="NGLFEDPFSSPPGRVVDYVGGVEDIRARVLRCIGEPDRRFEEDS
                     LRLMRAVRFAVTREVQVEKETCSSIFRNAPLLARISPERIREELDRILLSPGRKRGVE
                     MLVETGLMKHVIPEIYDMIGCTQPPQWHPEGDVYTHTLMMLDALGRDGSPVSLELALG
                     VLLHDIGKPPCRQVDETGRIRFSGHDKEGSSMARVILRRLKYSNAVVDAVCSMVERHM
                     RFINVRQMRKSTLRMFMNAPHFRDELELHRLDCLSSNGLMDNWQFVRDFMDSYRNAPL
                     VPPPLVTGRDLVNLGLSPGPDFGKWLSRLQELQLEGTLRTREEALLQLEQIAPVEQNA
                     LAEYLKKLAL"
BASE COUNT         2046 a         2643 c         2372 g         1939 t
ORIGIN      
        1 acagattcat cactgctgac atccagatca taaacgggaa catcctctcc gaagttctca
       61 tggatcagct tgagaactcc gcccttgaga cgttctccct gataattgaa gatgagcttt
      121 gcgcccgctt catgccatgc cttggcaatt gcgaacgcga tgctgcgctt gttcgctacg
      181 ccgactacga caccaacttt gccttcgagt aatttacttg acataatggt aactgaatac
      241 tgccttactt tccttcattt gacaagaaaa acggacgtca cggcattttc gtcaggctcc
      301 cgtctgctgg cggcacgctt tgaccgtatt agccatcaac atggcgatag tcatcggccc
      361 cacgccgcca ggcacgggag taatggcccg gcacttggga gccacctcgt cataggctac
      421 gtctcccaca atccggtagc cgcgcttggc ggaggcatct tccacacggt tgatgcccac
      481 atccaccact acggcgccgt ctttcacaaa atcagccgtc acaaaagcgg gccggcctat
      541 ggctgccacg atgatatcag cggaacggca aagttccggc aggtttttcg tacgggaatg
      601 agccacggtt acggtagcgt ttgcctgttt ggacatcaaa agatgcgcca tcggtttgcc
      661 cacaatcatg ctgcggccaa tgaccacggc attcgctccc gccgtttcaa tgccggcggc
      721 ctttaacagc ctcatacagc ccagaggggt acagggcaca aaaccggtct catcttccag
      781 caccagcttg gcgacattct ccggatggaa gccgtccaca tccttggcag ggtctatttc
      841 caggaccacg gccgcctcgt cgatatgggg aggaggcggg ctttgcacca gtatgccgtg
      901 gacggcagga tccacgttca attcacggac aatccggagc agttcttcct gggaggcgtc
      961 ggaagggaga actattttac gggaattcat ccccagctcc gcacactttt tcaccttgga
     1021 atttacatat acctgggaag cgggatcttc ccctaccaga accacggcca aaccgggcgt
     1081 tattccctgt tccctgagcc gggcaatatc cctttcaacc tcttccagca cctgggaagc
     1141 tacttgtttt ccatcgatga tttgcataat aatgattggt taagaggcgg gaagtccgga
     1201 agcccctcgc caagcagccc ggccagaaga cgggcataat ccgccacctg ttcatccgtc
     1261 gcgtccggag gaatgaacag gcgctttttc catagaatat ttacgttgga acacggtttg
     1321 ggaatggcat agcggtccca ggtcttgttc aaacgccagc agctttcata ttcgatgcat
     1381 acgggaacga tgggcagccc tgactgggag gccagtttga caataccgtg gtgcacttcg
     1441 taaatagggc ctcgcggacc atccggagta aaacagaagc agacgtcccc ctcctggacc
     1501 tttcgcatca tttccatgta ggccagagcc ccccgccgtc cggaagatcc ccggacggca
     1561 cccaggccaa aatgcctgca tacggcctcc accagggcgc cgtccttgct ggcgctggta
     1621 agcacactca tcttgagcgg cttctgggcc agggaccacg cataacccgg aaccatcgtg
     1681 cggttgtgcc aaagggctac cagaaaaggc gccgtataaa catcatcatg ctcctccttg
     1741 atggaacgga tattcaggct catgcaaatg ggctggagta cggaccacag gccaaatccg
     1801 gccagcctgg tccaccagcg ggaacgcttt tcagatgttt tccgaacaat aggattgagc
     1861 agcagcatgg agtcctggca atatcacatg aatccgtcta ccgtaatacg gggaggagag
     1921 gaatttgacg gagctggaag agctattcca cgggaaggag cggtatgcat ttccgggcgg
     1981 cccgtgcgcg gcgcctcttc ctggaacatt ccggcagagt tgtcctgtgg agactccgga
     2041 gtaccggaaa ttttcacagg aagaataatt tcatgccctt cctgcgtcac gccggaaggc
     2101 agtttccggc tgtccaccat gtaggaagga attttaaaaa ccagcttccc cgtcaaggaa
     2161 gtaccgttac tgtacacccc cccggtcact cccagaaagc cctgctcaaa cagggaaatt
     2221 tnnnnnnnnn naacgacccg ttccatcgct ttccagcagg aaagaaccgg acgtaaagac
     2281 gggagaagag tacggcagcc ttatttccgt attccgggca gccatggccg ccagcaggcg
     2341 caaaacaggc agcctgatca tggtcgcctt gataacggaa ccgctgtatc cttttaggga
     2401 gggcctcttc tgttcagttg ttacaggcaa agcaaaatcc agcttcaatt catgcttttc
     2461 atccccttcc tgttctcccg tctgcacctt gagaagggaa gagaaatcat ctcccagcag
     2521 ggaagcgaga tcgaaatttt ttcctgtcaa agtccacgcc tggtgacgga aatccgtacc
     2581 cagaagaaaa ttccccgtaa tcatgaaggg gctgagatcc gtaggcttat tgagcaggcg
     2641 ccctttgtcc agaaaaccgg aaaaagtgag ctgcttgacg ccgctcccgt ttagaatgac
     2701 ggaaccggaa tcaaccccca tcagcggcca gcccttgatt ttcaattttt gctgtgacag
     2761 atccagcacg tattccggtt caggtccgtt cccggacgtg cggatgtagg cttccgccgt
     2821 catataaaag ggagcgccat tcctctccag agtaaattct tccgtcccat gggcataccc
     2881 cacttccaga cgtggcgctg tgtatcgccg gaaaacgaac ggcaattccc gtttcgtctg
     2941 cggtacggca aatttaacga atccgggccg caaaccaact accgccctgg acacattaag
     3001 gctttccccc ttgattttcc ccatgaataa gggcacggcg gaaatttccc cggagatgcc
     3061 ttccatcgcc acaaaggaca ggcaggaatc ttccggaaag gataccttga tgcgtgaagc
     3121 cttcatggta aggccatcca ccgccattcc ggaaattttc acttctccgc cccaggcggc
     3181 ggaaagatca gcttgcagac ggccggcaaa ttcctccgta ttgaaagaag taaggcggta
     3241 catatacgtc cccgccagcg caccggcaac cagcagaacc agaatgcaaa ggcctattaa
     3301 aaaacgcttg tttttcaaca tggcctgttt ggcgtcccgc tgatcgtcac tgtaagtccg
     3361 ccggcgcttt tccacccgga tcaccttgct gccgtccggg cgcactacta cttccctctt
     3421 tcgctccacg ccctgggaat ccgacgcatg cagctgacgc atgatattat cagccccctg
     3481 ggaaggagaa gactcaaaat cggaaccgct gtttttcaat ggtcttgatc cggttaaagg
     3541 ttactggaca ttgctgaggg cggggagaga agcgtcccct gccgggacct cctcgtccgg
     3601 aatggaagta tccggcgtat aaagcttttt gccgcccgga tccaccactt ccgcctttac
     3661 catgatgatg atggccttgc gctgttcctt ggaccccttg gaacggaaaa ggcgccccac
     3721 ccagggaata tcccccagaa cgggaatttt atcctcatac gtaatggaag tggatttttt
     3781 caaggcgccg atgacgagcg tattgccggt ggctatgcgc accggagcgg tcaggcgccg
     3841 cgtggaaaac acgggctgaa ggatgaagtt gtctgaagtt ttcaccatga cgacatcttc
     3901 ctttgcgtcc ttttcctggg agaccatggg aacgaaaagc gacgtaccgt aattgatgaa
     3961 accgtcaaaa tccacgattt ccggcactac ggtcatttcc acgatggatt tgtcatcgct
     4021 gatgccggtc acttccacat tgaagatcgt gccgagctgc ctggtttcaa aggcagtggg
     4081 atgggcggga gtcatgggca tgaccggaat accgccgccg ttattgtcat tgtcgcccca
     4141 gtcattatta ttgcccgtgc tgttgggaac ttcaggcgga tcatattccg tagggtaaat
     4201 cagttccctg ccgctgaaaa aggtggcttt ttcaccgggg cgcacaatca cggaaggatg
     4261 ctgcataatg tctgtgcctt ttttctggct caggccgcgc ataatcatcg tgatatccgc
     4321 gtgactccac atgccgcgca aagtgacgat gctgggagcg ccgccgggaa cataggtcgc
     4381 atcagaactt ctggcggaag tgccgcgttc tatcatgctg tcaatgctat cttccgtaaa
     4441 aacctggttg ccggaacgca gcccgcccac cactcctgcg ggagcgacgg ccccggccac
     4501 attggcggca ctgtccagca cggaattgtt atagtcattt ttatcggttc ccgcgccgcc
     4561 catgaaccac ttggtagggt ccaggttcag attgacgatc cagttaaagc ccaattcctc
     4621 caagtccgtc tgattgactt ccagaaaggt cgcgctgacc accacctgga ggggctgttc
     4681 cgcagtcctg gccgctacca gctcctccag catgctcaaa tctctgggcg tgccatggaa
     4741 aagaagagtg gagttgcccg agttgtattt aatgaaactg ccttccggga atttcacccc
     4801 catggaagcc agcgcctttt gcggatccac ccgtttcaag gtcatgccgg aagaaccgga
     4861 atcaccggaa ctgaaggggt ctgaattttc ggcgccagag tcctccgaca atccggaaaa
     4921 gaagcccggc ggaagggtaa tggacttggt gaccatgtag gaagttccgt ctgaactgga
     4981 taccagttcc gcaccaaagg catttgtccg cagaataagg ccggatgcgc gcgccacgta
     5041 ttccagggcc tcacgaagag gtacattctg aagattaagc gtaattctct tggcggcggc
     5101 gtccctggcg gaaacgctgt ctgcaggacc gggatccacg gaaatattca cgccgcgttc
     5161 cgccgcagtc atggccgtgg catcatgcgt cctggcctgg ctgcgcagat agtccacggc
     5221 ttcctgcacc gtcgctccat ccagctgcac acggggcagg cggatcatgc ccagtttctg
     5281 gtccacattc accaccgggt tatccagcgg ccgggaaaat gaagcgggtt cctccggtgt
     5341 ttccgtcaaa ggaacgggca tctcccactg tctggaaaca tccgccaggg cactgctgcg
     5401 ggtttcatcc ctggcagcgc taaaataggc ggttctggcg cggttaacca gttccatgcc
     5461 gcgccgcgcc gccgtattgt aagggtccgt cttaaggacg gacgtaaatt ccttcagggc
     5521 cgcatcatac tggcccagtt cataatagcc gaaagccagg tgaagcagac ggttgacttc
     5581 ctctacattt tttacgtgtt ccggagacaa tgccggattc gtacgcaccg ggtcgcgggc
     5641 tatttccaac gtgcgcctgg ccaaggcatg gtccggagtg gatttcaggg catcttccag
     5701 caacttgacg gcctcatcgt aacgccccgc cttgatatat tcctgggcca cggccacgct
     5761 ggcatccgcg atgctggttt ccaggaaacg gcggcgcttc tccatattgg gggatttggg
     5821 cagcgcagtc aggcttctgc ggtaattgtc cagagcttcc tggtacttgc cctcggaata
     5881 ttgctgacgc gcctgcccta acagcagcat ggaagcctga gcctgctctt ccatccgggc
     5941 tgctgcccgg cgtgcggccc ctgccccctc ctgggccagc ccctggccgg ctccaatacc
     6001 ggcaacggca agcgccatgg caatttgctg aatggcagac atggagtgag ggaaacgtct
     6061 agtcatagga atttccggga ctataccgaa ttaaaaaccg cccgtcatca taaaaacgcc
     6121 cagcagagta aaaaatatcg gcaaaggctg cagcttccct ccggagggaa cgtttcttta
     6181 aacggcagac agttccagca cgcccacatg tcccggtttc aggggatggg acagaccgta
     6241 gtccatcaat tcccgccgct caaaaatcct gtcctcaccc tcggcgtcca tcaggtttct
     6301 gacgcgtacc cgatccgtct ggatgccgca ccagccgaaa gcgtgctggg gaaaggaaat
     6361 gcgcaggttg tagaaattga tatttggaga aaggttcccc accacaagca cggtggccct
     6421 ggcgcgggca tcatgccgga gcatggcata tacccagtgg ccggaagtgt cttccgccgg
     6481 ttccctgccg aaagtggtgt ttttcatatt agcccagttc aggccgtaaa aatctccccg
     6541 gtccagcgcc ggatgctgca tcagaggaag aagcttccga tgaaaatttc tcagttctgc
     6601 ggactcttcc ggcaacaggg aagagtcaaa ccggccattc gccacccagg gctggagccg
     6661 ggggaggcag gtatagtcaa aaatactggt acgtccgtca tgtccgccga aaccgccggg
     6721 cccctccgcc cgttccccca cttcctgccc gttgtacacc agcacgggac cgctcccgga
     6781 ggcatacacc agcgtcatga ccgccggcag aacaacgcgt cccacgccgc cccaggaaag
     6841 aggggagcac acgcgcgttt catcatggtt ttccacgtag cggacgccgt gggtcatgta
     6901 tatgggattg cttcggaaaa gccggtcgaa gtcattggcc cagttatttt ccgtgtacat
     6961 atggagcgcc agatgatagc agtcggaatc gtaaacggca ttaaatccgg catccagaag
     7021 ggcggcgcac ggatcgccgg gtgtggtttt catgtggtcg ttgtatgcct ccgccatgaa
     7081 aaacacgtcc ggcagacgca cccgggaacg ggaaatggcc catttccaga aagccatggg
     7141 gatcatttgg gccatgtcgc acctgaaacc gccgatgccc aggctttgcc agaaggaaag
     7201 gatatcgtcc atgatgcgcc atgtcttggg cacatgctgc ttggggctgg tccaccccgg
     7261 caacaggcgg agggcaggaa gaccggccat aaagttatag ccgtagttga gcttgaccgt
     7321 ttcataccag tcgtacttgc cgggattcca tgtaacggca ttgttgcccg tcacacgtcc
     7381 gaacgtcatt tccccttcaa acaggccgtc cggcagacgc aggggtggac cgtccccagg
     7441 gctgttggag gtcaggtaaa agtacccctg ctccggagaa aaaaacgtat ggggatcatc
     7501 tccttctcca aaatcgtcat gcccgtccca gtccgccagg taggctcggg aaacatggtt
     7561 gggaataaaa tccatcatcg gcagcagccc caccgtccgg caacgcttta ccagagcctt
     7621 gaactcatcc attctcttct ccggggagga ggccagatcc ggatccacgt caaaataatc
     7681 caccacggca tagggacttc ctgccaggcc ttttacgatg gatttcggct gggcggctag
     7741 cccgggatgg gacgtctgcg tagcgtgcct cagcaccccg gtcagccaga tgtgtgtgaa
     7801 acccatccgt gctatttcac gtaaggcttt atccgtcact ccattaaaag ttccgcaccc
     7861 gttcgtttcc cggctccccc agtcaactcc atgggtctcc atattggaaa aatgacgaac
     7921 aaatagttga taaatgacag gacgcatgag ttgaaataat caaattttga cgcttataat
     7981 gctaactttt tcaggtattc tgcaagcgca ttctgttcca ccggggcaat ttgttccagc
     8041 tgcagcaggg cttcctccct ggtccggagc gttccctcca actgaagctc ctgcagacgg
     8101 gacagccatt tcccgaaatc aggccccggg gacagcccca gattcaccag atcacgcccg
     8161 gtcaccagag gaggaggcac cagaggggca ttccggtagg aatccatgaa gtccctgaca
     8221 aattgccagt tgtccatcag tccgttggag gaaaggcaat ccaggcggtg gagttccagt
     8281 tcatcccgga aatgaggagc gttcatgaac attcgcagcg tagatttcct catctgcctc
     8341 acgttgatga agcgcatatg gcgctccacc atggaacaca ccgcatccac cacggcattg
     8401 gaatatttca gccttcttaa aatgaccctg gccatggaag agccttcctt gtcatgaccg
     8461 gaaaaacgga tgcgccccgt ttcatccacc tgacggcagg gaggtttccc gatgtcatgc
     8521 agcagaaccc ccagggccag ctccaaagac acgggggagc cgtcccttcc cagcgcatcc
     8581 agcatcatca gcgtatgggt gtatacgtct ccttccggat gccattgggg aggttgcgta
     8641 cagcctatca tatcgtatat ttccggaatg acatgcttca tcaaccccgt ttccaccagc
     8701 atttccacac cccgtttcct tccgggagac aataaaatcc tgtccagttc ctcccggatg
     8761 cgttccgggg aaatccgggc cagcagcgga gcgttcctga aaatggagga gcaagtctcc
     8821 ttctccacct gaacttccct ggtaacggca aaacgtacgg cgcgcatcag ccgcagggaa
     8881 tcttcttcaa aacgacggtc cggctccccg atgcacctca atacgcgggc ccggatatcc
     8941 tccactccgc ctacataatc caccacacgg cccggaggcg aagaaaacgg atcttcaaag
     9001 aggccgttca
//
LOCUS       DAOMSU010000066         8950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_544_length_8950_cov_2.691849, whole genome shotgun sequence.
ACCESSION   DAOMSU010000066 DAOMSU010000000
VERSION     DAOMSU010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_544_length_8950_cov_2.691849"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PK077_04350"
     CDS             <1..216
                     /locus_tag="PK077_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00268 family protein"
                     /protein_id="HRN23561.1"
                     /translation="ESFLRTLGLKGCRVRAHGDSLARIELPEKERRLFWDGQLASTAA
                     RRLRELGFRHITLDLEGYSRGSMNEPS"
     gene            213..968
                     /gene="larB"
                     /locus_tag="PK077_04355"
     CDS             213..968
                     /gene="larB"
                     /locus_tag="PK077_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRN23562.1"
                     /translation="MNDITSILHQFKKGLLSAEEAAEKIRAGTVPAPSHTDIDYSRLE
                     RTGCPEVIYGAGKTPDQIEEIARSLLAAGQNVLATRLNGEALDYLNRAFPAADMHPEA
                     RLMRIAPNPVPRTAGFVGIVSAGTSDQYVAEEAALTAEFLGSRVVRYRDCGVAGLHRL
                     VSHLDSIRQATALVAVAGMEGALPSVLAGLVKVPVIAVPTSVGYGANFRGVTTLLAMM
                     NSCANGVSVVNIDNGFGAGFNAHLINSLASQPS"
     gene            1066..2526
                     /locus_tag="PK077_04360"
     CDS             1066..2526
                     /locus_tag="PK077_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HRN23563.1"
                     /translation="MTRFPLFHAFSCSLLALASQALGWQTSGESVPAVPQEFRAAWIS
                     TVHNIDWPSRSGLSGAAQRAELLNILNTCAQLKLNAVFLQVRPNADALYRSSLEPWSQ
                     WLSGPGVNPGYDPLAFAIQEAHRRGIELHAWFNPFRAKANVKHAVGRNHISLTRPDLM
                     KRNGSVLLINPSASASRDHALKVIMDVVRRYDIDGVHLDDYFYPYPTPGRAWSPASFG
                     DGKSPSQRRGYIDDFVQDMYKSVKSSKPWVRVGISPFGIWRPGVPGGIEAGVDAYEHL
                     ACDARKWLSRGWVDYLAPQLYWRCSPAKQSFPALMQWWAAQNSRRPVWPGIATARIMS
                     SEDPGRPASEIAAQVNYSRSLARTAPGQCFWSIKSIMRNAGGIQKYLNRLYPSMAVPP
                     AMPWCGTGTPGQPQNFYVADNGSTVTLSWQPSGNPSRKWAVQARYGSQWATRILLPGS
                     QTRVTLPKSFLGDAGSVAVRGVSVYGAQGPAAAARR"
     gene            2743..3387
                     /locus_tag="PK077_04365"
     CDS             2743..3387
                     /locus_tag="PK077_04365"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRN23564.1"
                     /translation="MTKQQDPSTIGYADGKYVLPPLPYAYDALEPMLDEKTVRIHHDK
                     HHAAYVAGANAAAEKLREIADGKLDASATTNWVRNLSFNASGHVLHTIYWTNMTPDPK
                     KEPQGPLVDAIKEKFGSLEAMMKEFKAASQGVEGSGWGILGVDPISKTLVICGAEKHQ
                     NLEIPGLVPILVCDVWEHAYYLKHQNLRADYIEDFCRLINWDDVERRYQEAMAS"
     gene            3537..4628
                     /locus_tag="PK077_04370"
     CDS             3537..4628
                     /locus_tag="PK077_04370"
                     /inference="COORDINATES: protein motif:HMM:NF013621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HRN23565.1"
                     /translation="MKKVFVSGCYDIVHAGHIQFFEEARALGDYLIVSFASEPVLWHH
                     KQRKPSIPDEHKKVLLESLRMVDKVILGTGMKKGLDFEEEFLQEKPDILAVTEDDLYS
                     DIKKELCARVGANYVVLPKTPPKFTPVSTTMLVNRIKAPSAVPLRVDFAGGWLDVPRY
                     ARKGSYVVNCAITPMVSLCEWPYEKRSGLGGSGAWAMLEGRDPVASELALGVGWQDPA
                     VIAETGLCVWRSGSSPVLDVKGTGDFLEGRMAILYTGEEHDTPKMADEQRDYVRISQS
                     SLIARTGVLERNINTLAAGVALYYSVQLDEGMRPLPDIPNALAKKYLGGGYGGYALYL
                     FPCRDDRDQAVKDNPAMKRVEPYCRQLFK"
     gene            4642..6273
                     /gene="murJ"
                     /locus_tag="PK077_04375"
     CDS             4642..6273
                     /gene="murJ"
                     /locus_tag="PK077_04375"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRN23566.1"
                     /translation="MSLMRNSLVASGAIFACRLTGMAREIVYTSLFGATGALDAFYTA
                     FRIPNLLRDLFAEGALSQSYTSVASKTREAQGEAAAWELTNKVATQLSSLMIAIVTLG
                     ILFAGPVMEALYSGDHSLTEQLFATDLSRIMWPFIGFASLSALIMGALNMVGVFGLPM
                     LASAAFNVTSILLGLLIGYFIDPSFGPKALYGFACGVTIGGMAQIAVQLPKLSKTGFR
                     WKPNFQWDDPRVRKIWGLMLPSVLASGVTQFTIFINTGFALDLQKGSVTALTTAFRLW
                     QLPVGLFGVATGMVVLPAVSRMMVGDGRKEVAVHIAKGLRLVAFFAVPAFLILSILGT
                     EFVSAVYQWGRFNQEAVRYTGEVLGAYSLGLHLNGRRGGLCPAPGAGCRRDAGLGYAG
                     TKVVQPVFLALEKRWVPLIAAAVALAISIGLNYCFVYILHKNAAWLALTTSVVTTFNF
                     LFYFFYLRRQLGGVDGRTLLSGLVKIMAAGILLGAVCWAGKAWFLQGFLNWTFPARVL
                     GISLVCGCAGIVYLAAAFLLKTPELEAVRAKFLKR"
     gene            6449..7609
                     /locus_tag="PK077_04380"
     CDS             6449..7609
                     /locus_tag="PK077_04380"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HRN23567.1"
                     /translation="MISPSIAALTPSLTLKVTNRAKAMKASGEEVYGLAGGEPEMDTP
                     ENIKQAAIAALNNGATKYTPSAGLPALRQAIAEKLKRENNLEYDFTQITVGAGAKHAC
                     FNAIMATVSEGDEVIIPAPYWVSYPEMVRMCGGIPVIVETTAENGWKLTAEQFEEAMT
                     PRTKMVILTTPNNPTGSVYSEEELRALGEVALSEDILILADEIYEHLVYGDTKHVSIA
                     SLSPELYDLTITINGFSKGYAMTGWRLGYSAAPAAIAKAIQMIQDHTTSNVCTFAQYG
                     GIEALTGDQSFISDMRDEYDMRRQYVYSRLSAIPNISVVEPQGAFYFFVDTSRLGLTS
                     LNLCDKLLERYKVAAVPGIAFGNDKAIRISYCTTLDILKEALDRFEEFCKAH"
     gene            7766..8275
                     /locus_tag="PK077_04385"
     CDS             7766..8275
                     /locus_tag="PK077_04385"
                     /inference="COORDINATES: protein motif:HMM:NF017902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HRN23568.1"
                     /translation="MNTPVLNDNLRAATEALCNLLAKEDKVVASKAKIGLFFQNPEAT
                     KLFEEVNAYGEELRNKHLAGMPPTEEEISKFDALRENVIKNDAARGFLEARQTIDELL
                     NTINHYLGMSIDLGRAPTPEEIEEARQRAMSAQSSCSCGGQCDKENCDCDGKCDHDHK
                     DGGCGCGNH"
     gene            8538..>8950
                     /locus_tag="PK077_04390"
     CDS             8538..>8950
                     /locus_tag="PK077_04390"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HRN23569.1"
                     /translation="MTFSNRPAPLPFFKTDFPLFLAPMAGVTDPIFRTICKELGVDVM
                     VTEFVSAEGILQAWERNRRYTKIENAHRPIGIQLFGADGNHMGEAARIILDHEKPDFI
                     DINFGCPVPKVVGKNGGSSLLKDLPLLASVAGGVV"
BASE COUNT         1929 a         2774 c         2410 g         1837 t
ORIGIN      
        1 gaatctttcc tgagaaccct gggtctgaaa ggctgccgtg tccgtgctca cggagacagc
       61 ctggcccgca ttgaattgcc ggaaaaggaa cgcagactgt tctgggacgg acagcttgcc
      121 tccaccgcgg cacgccggct gcgggaactg ggcttccggc acatcaccct ggatctggaa
      181 ggatattcca gagggagcat gaacgaaccc tcatgaacga catcacctcc attctgcatc
      241 agtttaaaaa aggcctcctt tctgcggagg aagcggcgga aaaaatcaga gccggcaccg
      301 tacccgctcc ttcccatacg gacattgatt acagccgcct ggaacgcacc gggtgcccgg
      361 aagtcattta cggcgcagga aaaacgccgg accagattga agagatagcc cgcagcctgc
      421 ttgccgccgg acaaaatgtt ctggccacgc gtctgaacgg ggaagctctg gactacctga
      481 accgcgcctt tccggcggcg gacatgcacc cggaagcccg tctcatgcgc attgctccga
      541 atcccgttcc gcgaacggcg ggcttcgtcg gcatcgtcag cgcggggacc tcggaccaat
      601 acgtagcgga ggaggccgcc ctgacagcgg aatttctggg cagccgggta gtccggtacc
      661 gggactgcgg cgtggccggc ctgcaccgcc tggtttccca tctggactcc atccgccaag
      721 ctactgcgct ggtggccgtg gcgggcatgg aaggagctct ccccagcgtg ctggcaggcc
      781 tggtaaaggt tcctgtgata gccgtgccta ccagcgtggg ctatggagcc aatttccgcg
      841 gggtaaccac cctgctcgcc atgatgaatt cctgcgccaa cggtgtttcc gtcgtcaaca
      901 tagacaacgg cttcggggcc ggtttcaacg cccacctgat caacagcctg gcatcccagc
      961 ccagctgagg gccgcctgtc catgacgaaa cctccggcat ccgtcatgga cagatcctcc
     1021 gaataggcgt taaatattcc ggaaacatcc ctctccattc ccgtcatgac cagatttccc
     1081 ctgttccatg ccttctcctg ctccctgctg gccctggctt cccaggcgct tgggtggcag
     1141 acctccggag aatccgttcc cgctgtcccc caggaattcc gcgcggcatg gatatccacc
     1201 gttcataata ttgactggcc ctcccgttcc ggcctctccg gagcagccca acgcgcggag
     1261 ctgctgaaca tcctgaatac ctgcgcccag ttgaagctga acgccgtgtt tctccaagtg
     1321 cgcccgaatg ccgacgccct gtaccgctct tctctggaac cgtggagcca atggctttcc
     1381 ggcccgggcg tcaatcctgg gtatgatcct ctggctttcg ccattcagga ggctcaccgc
     1441 cgcggcattg aactgcacgc ctggttcaac ccattccggg caaaggccaa cgtcaagcat
     1501 gccgtaggcc gcaaccatat ttccctcacg cgccctgatt tgatgaagcg aaacggctcc
     1561 gtgctgctga taaaccccag cgcctccgcc tcccgcgacc acgcgctgaa ggttatcatg
     1621 gatgtcgtgc gccgctacga cattgacggg gtgcacctgg acgactattt ctacccctat
     1681 cccactcccg gccgcgcctg gtctcccgcc agctttggag acgggaaatc cccctcccag
     1741 cgccggggct acattgacga cttcgtgcaa gacatgtaca aatccgtcaa atcttccaaa
     1801 ccctgggtgc gggtgggcat cagcccgttc ggcatctgga ggccgggcgt tcccggcggc
     1861 attgaagcgg gagtggacgc ctacgagcat ctggcgtgcg acgcccgcaa gtggctttcc
     1921 cgcggatggg tggattacct ggccccccag ctttactggc gctgcagccc ggccaagcag
     1981 agctttcccg cattgatgca atggtgggcc gcccagaact ccagacgccc ggtgtggccc
     2041 ggcattgcca cggcacgtat catgagcagc gaagacccgg gccgccccgc ctctgaaata
     2101 gcggcgcagg tcaactactc ccgcagcctt gccagaaccg cccccgggca atgcttctgg
     2161 agcatcaaat ccatcatgcg gaatgccgga ggcatccaga aatacctgaa cagattgtac
     2221 ccctccatgg ccgttccgcc cgccatgccc tggtgcggaa ccggcacacc cggacaaccg
     2281 cagaattttt atgtggcgga caatggttcc accgtaaccc tctcctggca gccgtccggc
     2341 aatccctccc ggaaatgggc cgtccaggcg cgctacggca gccagtgggc tacgcgcatc
     2401 cttctgccgg gcagccagac ccgcgtaacc ctgcccaaat ccttcctggg ggatgcagga
     2461 tctgtcgccg tcaggggcgt cagcgtctat ggggcccagg gcccggcagc agcagccaga
     2521 aggtagaaaa gctttttccc agctgcctct acaagaagaa aaatagaacc ccgaggttcc
     2581 tgttttttga acaactcaaa aacaacccat gtccaaacac ccatgcattc ccatggcgcg
     2641 gacatgaaca gaaggcattt tattgctctt tcctcattgg cggcgctggg aacaatcagc
     2701 caggcccggg caacagggcc atccatacaa ccacagcaca agatgactaa acagcaagat
     2761 ccctccacga tcggctatgc cgacggcaaa tatgtactgc caccccttcc gtacgcctac
     2821 gacgcgcttg aacccatgct ggatgaaaag accgtgcgca tccaccacga caagcatcat
     2881 gcggcctatg tcgccggcgc caatgccgcg gctgaaaagc tccgtgaaat tgcggacggg
     2941 aaactggacg cttccgccac caccaactgg gtgcgtaatt tgtccttcaa tgcttccggc
     3001 catgttctcc ataccattta ctggaccaac atgacgcctg atcccaaaaa agagccgcaa
     3061 ggtcctctgg tagatgccat caaggaaaaa tttggttctc tggaagccat gatgaaggaa
     3121 ttcaaggcgg cttcccaggg cgtggaaggt tccggctggg gtattctggg cgtggacccc
     3181 atcagcaaga cgctggtaat ctgcggggcg gaaaaacatc agaatctgga aattcccggc
     3241 cttgtcccca ttctcgtctg tgacgtttgg gaacacgcct attatctgaa gcaccagaac
     3301 ctgcgggcgg actatattga agatttctgc cgcctgatca actgggacga tgtggaacgc
     3361 cgctaccagg aggctatggc ttcctgaccg tttttgcaga agttgatttt tcctatcccc
     3421 aaacggcacg ggctttctgt agtccgcgcc gttttttaac gtattttcat ctcgctcttg
     3481 catccggcac ggcaataggg catactgttg cagactaatt atctatcatt acacgcatga
     3541 aaaaagtttt cgtctccggc tgctatgata tcgtccacgc cggccatatc cagttctttg
     3601 aagaagcccg cgctctgggc gactatctaa tcgtctcttt cgcttcggaa cccgtgctgt
     3661 ggcaccacaa gcagcgcaag ccgtccattc cggacgaaca caaaaaagtt ctgctggaaa
     3721 gcctgcgcat ggttgacaag gttatcctgg gtaccggcat gaaaaagggc ctggattttg
     3781 aagaagagtt tcttcaggaa aaaccggata tcctggctgt gacggaagat gacctttaca
     3841 gcgatatcaa gaaggagctg tgcgcccgcg tcggagccaa ttacgtggtg ctccccaaga
     3901 cgccccccaa attcactccc gtctccacca ccatgctggt gaaccgcatc aaggctccgt
     3961 ccgccgtacc gcttcgcgtg gactttgccg ggggatggct ggacgtgccc cgctacgcca
     4021 gaaaaggctc ctatgtggtc aactgcgcca tcacccccat ggtttccctc tgcgaatggc
     4081 cgtatgaaaa acgctccggg ctgggaggca gcggcgcgtg ggccatgctg gaagggcgcg
     4141 atccggtcgc gtccgaactc gctctgggcg tcggttggca ggatcctgcc gtcattgcgg
     4201 aaacggggtt gtgcgtatgg cgctccggca gttctccggt gctggatgtc aaaggcacgg
     4261 gtgacttcct ggaaggaaga atggccattc tatatacggg tgaagaacac gataccccaa
     4321 agatggcgga tgaacaacgg gactacgtac gcatctccca atcctccctc attgcccgca
     4381 ccggagttct ggaacgcaac atcaatacac tggcggcagg cgtagccctg tattacagtg
     4441 tgcagcttga cgaaggaatg cgccccctgc cggatattcc caatgccctt gcaaaaaaat
     4501 atctgggagg cggctacggg ggatacgccc tgtacctttt cccctgccgg gatgatcgcg
     4561 accaggctgt caaggacaat ccggcaatga aacgggtgga gccgtattgc cgccagctgt
     4621 tcaagtaaat tccccccttt catgtccctg atgcggaaca gcctggttgc ctccggcgcc
     4681 atcttcgcct gccgcctgac aggcatggcc agggaaattg tgtacacctc cctgttcggg
     4741 gctacgggag ccctggacgc cttttacacg gctttccgta tccccaacct gttaagagat
     4801 ctgtttgcgg aaggggctct ttcccaatcc tataccagcg tagcctccaa aacgcgggaa
     4861 gcgcagggag aagcggccgc gtgggaactg accaacaagg tagctaccca gctttcctcc
     4921 ctgatgatcg ccattgtaac gctggggatt ctatttgcag ggcccgtcat ggaagccctc
     4981 tacagcggag atcactctct gacggagcaa ttgttcgcca cggacctgag ccgcatcatg
     5041 tggcctttca tcggattcgc ctccctgtcc gccctcatca tgggggcgct gaacatggtg
     5101 ggggttttcg gattgcccat gctggcctcc gccgctttca atgttacttc catcctgctt
     5161 ggcctgctga ttggatattt cattgatcca tccttcggcc ccaaagcttt gtacggtttt
     5221 gcctgcggcg tcaccatcgg gggcatggcc cagattgccg tgcagctccc caaactcagc
     5281 aaaaccggct tccgctggaa acccaatttc caatgggacg atccccgcgt gcgcaaaatc
     5341 tgggggctga tgcttccctc cgtgctggct tcaggcgtga cgcagttcac catcttcatc
     5401 aatacggggt tcgctctgga tctccaaaaa ggctccgtca ccgccctgac cacggcgttc
     5461 cgcctctggc agcttcccgt ggggctcttc ggagtcgcga cgggcatggt ggtgctgccc
     5521 gctgtttccc gcatgatggt gggagacggc agaaaagagg tggcggtcca catcgccaag
     5581 gggttgcggc tggtggcatt ctttgcggta cccgcttttc tcatcctctc catcctggga
     5641 acggaattcg tctccgccgt ataccagtgg ggccgcttca accaggaggc cgtgcgttat
     5701 acgggggaag tgctgggagc ctattccctg ggcctgcacc tgaacggccg caggggcgga
     5761 ctgtgccccg ctcccggagc tggatgccga agagacgctg ggctgggcta tgccgggacc
     5821 aaggtggtgc agcccgtctt cctggctctt gaaaaacgct gggttcccct gattgccgcc
     5881 gccgtggctc tggctatcag catcggcctc aactactgtt tcgtctacat cctgcataaa
     5941 aacgcggcgt ggctggcact gaccacctcc gtggtcacta ccttcaattt tctcttttac
     6001 ttcttttacc tgcgacgcca gctgggcggc gtagacggac ggacgctgct ttccggactg
     6061 gtcaagatca tggcggcggg aatattgctg ggggccgtgt gctgggcagg caaggcatgg
     6121 ttcctccagg gcttcctgaa ctggaccttc ccggccaggg tgctggggat atccctggtc
     6181 tgcggttgcg ccggcatcgt ctacctggcg gcggctttcc tgctgaagac gccggagctg
     6241 gaagcggtgc gcgccaaatt cctgaaacgc tgacgggaaa aatcccttcc atagagggag
     6301 gaacgccctc cgcaagaaaa agaatggccg ccgcaagcat tctccttgcc aggggtgcgc
     6361 tcaatgagta aagtttttcc gcctatgcgg agaacagcct gttttccgcc aaggcatcaa
     6421 aatctacgac tgcaccacaa caatggacat gatttccccc agcattgcgg cattgacccc
     6481 ttccctgacc ctcaaggtga ctaaccgggc caaggctatg aaagcttccg gtgaagaagt
     6541 ttacggtttg gcaggcggtg aacctgaaat ggacaccccg gaaaacatca agcaggcagc
     6601 catcgcggcc ctgaacaacg gagccaccaa atatacgcct tccgccggtc ttcccgcact
     6661 ccgccaggcc atcgcagaaa agctcaagag ggaaaacaac ctggaatacg atttcaccca
     6721 gatcaccgtg ggagccggcg ccaagcacgc ctgtttcaac gccatcatgg cgaccgtatc
     6781 cgagggggat gaagtcatca tccccgcacc ttactgggtc agctatccgg aaatggtccg
     6841 catgtgcgga ggcatccccg tcatcgtgga aaccacggcg gaaaacggat ggaagcttac
     6901 cgccgaacaa tttgaggaag ccatgacccc ccgcaccaag atggtcatcc tcactacccc
     6961 gaacaatccg accgggtccg tttattccga agaagaactg cgcgccctgg gagaagtggc
     7021 cctgagcgaa gatatcctca tcctggctga tgaaatttac gaacacctcg tatacgggga
     7081 caccaagcac gtcagcatcg cctccctcag tccggaactg tacgacctga ccatcaccat
     7141 caacggcttt tccaagggtt acgccatgac cggatggcgc ctgggctatt ccgcagcccc
     7201 ggctgccatt gccaaggcca tccagatgat tcaggaccac accacttcca acgtctgcac
     7261 gttcgcccag tatggaggca ttgaggccct tacgggagac cagagcttca tcagcgacat
     7321 gcgcgacgaa tatgacatgc gccgccagta cgtttactcc cgcctcagcg ccatcccgaa
     7381 tatcagtgtt gtggagccgc agggcgcttt ctacttcttt gtggacacaa gcaggctggg
     7441 actcacctcc ctgaatctgt gcgacaagct tctggaacgc tacaaggtcg cggccgtccc
     7501 cggcatcgcc ttcggcaatg acaaggccat ccgcatcagc tactgcacca cgctggacat
     7561 tctgaaggaa gctctggacc gctttgaaga attctgcaag gcgcattaaa aaacgttgat
     7621 caccataaaa aggcgttcca cgtcaagcgg aacgcctttt tacttccgga tttcccgcct
     7681 gcagcaaccc tgcggcaagg aaatgcgcgc catgcctcgc aaccggattg ccccgcacga
     7741 aaaggcatga ttaaataaat ctcatatgaa tacccccgtt ctcaacgaca acctgcgcgc
     7801 cgccacggaa gccctttgca atctgctcgc caaggaagac aaggttgtgg cctccaaggc
     7861 aaaaataggt ctctttttcc aaaatccgga agcaaccaag ctttttgaag aagtaaacgc
     7921 ttacggagaa gaactgcgca acaagcacct ggccggaatg cctcctaccg aagaggaaat
     7981 ttccaaattc gacgccctgc gtgaaaacgt catcaagaat gatgcggccc gcggcttttt
     8041 ggaagcgcgc cagaccattg acgaactcct gaacaccatc aatcattatc tgggcatgtc
     8101 cattgacctt ggccgcgctc ccactcctga agaaatagag gaagcccgcc agcgcgccat
     8161 gagcgcccag tcctcctgct cctgcggcgg acagtgcgac aaggaaaatt gtgattgcga
     8221 cggcaagtgc gaccacgacc ataaggacgg tggttgcggc tgcggcaacc attaagacaa
     8281 tctttcagct tttgccaaac ggcttcactc aaaaggtgaa gccgtttttt cttcccgaaa
     8341 acaaacgaca ctgtccaacg cgtctgcaat tccctccgga aaaccgggcg gaatactgca
     8401 cctctttatt gagcgccata tttttcaacg ctccgatgct tgctcagtac cggcattttt
     8461 caccttccct tctccgccag aacaggcaag atgcttgaat ggaacgggaa cccgtgctag
     8521 cctcatccta aggaatcatg actttttcca accgtcccgc tcccctcccg ttcttcaaaa
     8581 cggatttccc cctgttcctg gctccaatgg ctggagtgac ggatcccatt ttccgcacca
     8641 tctgcaagga acttggagtg gatgtgatgg tgacggaatt cgtctctgcg gaaggaattc
     8701 tccaggcatg ggagagaaac cgcaggtaca caaagataga aaatgcccac cgtcccatag
     8761 gtatccagct cttcggagcg gacgggaacc acatgggaga ggcggccaga attatcctgg
     8821 accatgagaa gccggatttt atcgatatca acttcggctg cccggttccc aaggtggttg
     8881 gcaaaaatgg aggttcctcc ctcctgaaag atcttcccct gctggcttcc gttgccggag
     8941 gcgtcgtgaa
//
LOCUS       DAOMSU010000067         8897 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_550_length_8897_cov_2.475006, whole genome shotgun sequence.
ACCESSION   DAOMSU010000067 DAOMSU010000000
VERSION     DAOMSU010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8897)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8897
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_550_length_8897_cov_2.475006"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..2339)
                     /locus_tag="PK077_04395"
     CDS             complement(48..2339)
                     /locus_tag="PK077_04395"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRN23570.1"
                     /translation="MCLADAGIVRVTYFPGAVPEDEPSYAVSPGYSAPGAEIREYDEA
                     GVHVVETSLLRIRIRTEEQKVDFYDVATDEPLLTDEGGFGRESKDWTGDARVWIRKNL
                     QETEHFFGLGDKPCALNLRGKYFSMWGADHYDFHEESDPLYKSIPFFLSLRERKAYGL
                     LFDNTCRSYFDFGATDEKVLSFGSFGGLMNYYFIYDNTPLDIISAYTRLTGTPELPPL
                     WALGYHQSKWSYYPDKAVYNLVERFRGLGIPCDAVHLDHHYMERKEGFTWDKQNFPDA
                     EGMVRALEKDGVKTVLIVNPGVKVNSANPVWKEGMERNYFCRRSEGNLLSEEVWPGLC
                     NFPDFTAPAVRGWWADLFSRDIGKIGVRGLWNDMNEPVVFPDRTFPMDTRHEYDGMPC
                     SHEKAHNIYGQCMAEASWLGMKRHAPDRRPFLLSRSGFAGLQRFAATWTGDNRSSWEH
                     LKLANFQCQRLAASGISFAGADAGGFMGHPTPELFCRWMQMASFHGFFRNHSSGEFGG
                     QEPWVFGQEVTSYVKAAIEGRYRLLPYIYTQFRRYAETGMPVLRSLALQCFTNKDTYW
                     RGAEYFFGDHLYVIPIHEPQEGGRFLYIPEGVWYSYHTDSLMEDTGKDVWVKCPLSFL
                     PVYVRGGAVIPHWPVQQYMGELPRPPLTLDVWWAPEGEVASHLYEDAGDGYAYRNGEC
                     AVHGFLYRGGTNSLELDWNCEGDPCAFHESAEVVLHGLPAGISVSACMDGVPCCGVMR
                     EGRVWKIPVKDKFDTLSVCWPEE"
     gene            2665..>3528
                     /locus_tag="PK077_04400"
     CDS             2665..>3528
                     /locus_tag="PK077_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN23571.1"
                     /translation="MSTIRVLTLGWEFPPLVNGGLGIACLGLSKALAKKVDLRVIVPK
                     ADPSVIFDGFQLTGLNNVSYREVEQVDRKYSYDSFALVERAPIELDPYTTVEGGSGVV
                     QFTKEGRITFSKTHEADLQLFRNKEDLYAGDLALKVIQFSKIAVKVALQQDFDIIHAH
                     DWMTYLAGVEVKKATGKPLVVHLHASQFDRAGADARGWIYDIEKFGMEQADAVIPVSK
                     YTGTIASGHYAIDPHKIFPIHNGADPVKVFKGKKKFPEKLVLFLGRLTAQKGPGFFLQ
                     IAAKVLEQTDDV"
     assembly_gap    3529..3543
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3544..3922
                     /locus_tag="PK077_04405"
     CDS             <3544..3922
                     /locus_tag="PK077_04405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRN23572.1"
                     /translation="FKGVGDKFHFTGFLNKDKVNELLSITDIYCMPSVSEPFGLSALE
                     AAQFNIPAVISKQSGVAEVMKGALKADFWDVNKMAEHIVHLCTDEELYRKVVEQSTED
                     IKASTWDAAADKVIRVYEHVLNR"
     gene            3973..5160
                     /locus_tag="PK077_04410"
     CDS             3973..5160
                     /locus_tag="PK077_04410"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HRN23573.1"
                     /translation="MSNISLTFVAHQPNRLIHYDFFKIGEHAFYEDDDLNARVLSTVA
                     ERCYFPATRLMKQLIELTGGKFRFGLALSGVILEQALYHRPDLIAAFKDLAETGCVDF
                     LAMPYYNSLASVYSPQEFAEQIEEHRELLKKLFHQETDVLMNTGMLYSNAIAAQAETL
                     GFKGIMADGNPAMLKGFTSNEVFLAPWVYNTTTIFRNRELSNDLAIMRTDPEWSEYPL
                     SPTTFADWLTHQQGSVTTLSMDYETLGERQSDATGVFEFWRTMILACIDAGNRFMTPS
                     EVAQEIKPVSVCECTQEMTCSTFGTMSHWNGNVMQDEALRKIYRLEKPVKAAHDKDLT
                     HVWRKLQSADHFHYMQKENSFTPYASAFDAYIYYMNALADLQIRVKRESGKPVQPDPE
                     KAS"
     gene            complement(5322..5864)
                     /gene="def"
                     /locus_tag="PK077_04415"
     CDS             complement(5322..5864)
                     /gene="def"
                     /locus_tag="PK077_04415"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRN23574.1"
                     /translation="MEIAQYGNPVLKEKCRPVEHFDDSLKTLAENMLETMYAAEGIGL
                     AAPQVSIPIQLVVIDIPKEEESVTWLKVNGEDKELSDIMPLMFANPVLEPYGPMHPFH
                     EGCLSVMKIRASVVRPDFVKATVLLIDGREITIDCNGLLARCLQHECDHLNGILFVER
                     VSSAQKITLRNKLKRLALGY"
     gene            complement(5902..6330)
                     /locus_tag="PK077_04420"
     CDS             complement(5902..6330)
                     /locus_tag="PK077_04420"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HRN23575.1"
                     /translation="MQFYRKKARSMGVPIVPMIDILTILLIFFIVHTQWKKPQSLLKI
                     DVPGAEFMEGTPSSEQRAVLAVTGTSAISLNGRLVEMNELAAALEALKKENPGVKLQL
                     DVDKKAEFGIIVGIWDALTSVGIDAGEVPARIEINKPGAR"
     gene            complement(6338..6970)
                     /locus_tag="PK077_04425"
     CDS             complement(6338..6970)
                     /locus_tag="PK077_04425"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRN23576.1"
                     /translation="MMMNLIKDCITFFQAGGVFMYPLAACSVLLIAAVIYRMFNMKKS
                     LICPAVLTRAVEQYTRGAVERTELERIAEDSDSVEGRLVLDALNSPLEDEASLKEMIQ
                     VKAREEFVTLQAGLPLLDMIVMIAPMFGILGTASGLVQIFSVFGMDESHGMIAQGIAQ
                     ALNTTIAGLAIATPAVIAHVYYSRKLERISASMEVLLTELVSFRCHHSQR"
     gene            complement(7009..8157)
                     /locus_tag="PK077_04430"
     CDS             complement(7009..8157)
                     /locus_tag="PK077_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23577.1"
                     /translation="MLAHLFLLAGSCLVPEEWLASPRPAEAARRKVAVSARMVKKVEP
                     QLESRRQEPAEYLPAVVKTNEEQESATRPEVPKFQSNRNTRAEGGRKDPDGDRDMPAQ
                     DGVKRETGEIVLFNQERQDGDLSHERDGNKNASPGASSNVPYQPLEPSMPPAGHPGSL
                     AHSPVKKGEPGKSEEEKTARQNIRHAQEKAMQLPHAVVLPSPGDSEKPDPLEELAKSI
                     AKGADNPPLNLPNLDLVPNTKPVQKQPLYDPMFNAESQPGFKTHERKTKQVGKFSFGR
                     RPTLDVEATPMGTYQAIVYRAIGEQWYRQCDLNRDLIVTGTVRIRILINKNGKISSMR
                     QTSRVGASEVQKSFTFLAIKLARLPAMPPEVRSMLVGDSLEMYFDFNF"
     gene            8419..>8897
                     /gene="ssb"
                     /locus_tag="PK077_04435"
     CDS             8419..>8897
                     /gene="ssb"
                     /locus_tag="PK077_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRN23578.1"
                     /translation="MANLNKVFLMGNLTADPELRYTPKGTAVTDIRLAINRYYAGDNS
                     ERQEETTFVDVTLWNRQAEVAGNYLSKGRGVFVEGRLQLDSWEDKASGQKRTKLRVIG
                     ENIQLFPRGGEGDMGGAPRQQYQNAPRSNNYGQSRPAQNYNPPPMPPTNQPSNDFGD"
BASE COUNT         2099 a         2590 c         2243 g         1950 t
ORIGIN      
        1 cttccatgac agttcctccg gacgcctcat cccttaatgg aagaacatta ttcctccggc
       61 cagcagacgg aaagcgtgtc aaatttatcc ttcaccggta ttttccacac ccttccttcc
      121 ctcatcacgc cgcagcacgg cacgccgtcc atgcatgcac tgacggaaat acccgcaggc
      181 aaaccgtgca aaaccacctc tgcggactca tggaacgcac agggatcccc ttcgcagttc
      241 caatccagct ccagggaatt ggtgccgccg cggtaaagga acccatgcac ggcgcattct
      301 ccgttccggt atgcatatcc gtcccctgca tcttcataca aatgggaagc cacctcccct
      361 tccggcgccc accatacatc cagcgtcagc ggcggccgag gcagttctcc catgtattgc
      421 tgtaccggcc aatggggaat taccgcccct ccgcggacat acacgggcag gaaagaaagg
      481 gggcacttca cccatacatc ctttcctgtg tcctccatca ggctgtccgt gtggtaggaa
      541 taccagacgc cctccggaat gtagaggaac cgcccgcctt cctgcggctc atgaatggga
      601 atgacgtaca gatggtctcc aaagaaatac tccgcccccc gccagtaggt gtccttgttg
      661 gtaaaacatt gcagggccag ggagcgcagc acgggcatgc ccgtctccgc gtaccggcgg
      721 aactgcgtgt agatataggg aagcaggcgg tagcggccct ctatggcggc tttcacgtaa
      781 gaggtgactt cctgcccaaa aacccatggc tcctgaccgc cgaactcccc ggaggaatga
      841 ttgcggaaaa aaccgtggaa agaagccatt tgcatccagc ggcaaaacaa ttccggcgtg
      901 ggatgcccca tgaatccccc agcatccgct cccgcaaagg aaatgccgga agccgcaagc
      961 cgctggcact ggaaattggc cagcttcaaa tgttcccagc tggaccggtt gtcccctgtc
     1021 catgtggcgg caaagcgctg caatccggca aaaccggatc gggacagcag gaaggggcgc
     1081 cggtccggag cgtgacgctt catgcccagc caggaggctt ccgccatgca ctgcccgtaa
     1141 atattatggg ccttttcatg ggaacagggc atgccgtcat attcatgccg cgtatccatc
     1201 gggaaagtgc ggtccggaaa aacgacaggt tcgttcatgt cgttccagag gccgcgcacg
     1261 ccaattttcc caatatccct gctgaacaga tccgcccacc agccgcgtac ggcgggagcc
     1321 gtaaaatccg ggaaattgca aagtcccggc catacttctt ccgacaataa attgccttcc
     1381 gaccggcggc agaaataatt gcgttccatc ccctctttcc agaccggatt ggcggaattg
     1441 accttcacgc cgggattgac aatcagaacc gtcttcacac cgtccttctc cagggcgcgg
     1501 accattcctt ccgcgtcggg aaaattctgt ttgtcccagg taaatccctc cttccgttcc
     1561 atgtaatgat ggtccagatg cacggcatcg cacggaatgc ccaggccccg gaaacgctcc
     1621 accagattgt atacggcctt gtccggataa tagctccact tggactggtg gtaccccagc
     1681 gcccacaaag gcggcaattc aggcgtaccg gtaaggcgcg tataagcgga gatgatgtcc
     1741 agaggagtgt tgtcgtaaat gaaataataa ttcatcaggc ccccgaagga cccgaaggaa
     1801 agaacctttt catccgtagc tccgaagtcg aaatacgaac ggcacgtgtt gtcaaacaat
     1861 aggccgtacg ccttcctttc ccgcaaactg aggaaaaagg gaatactttt gtaaagggga
     1921 tccgactcct catggaaatc ataatggtcc gctccccaca tggagaaata cttgcccctc
     1981 aggttcaagg cgcacggctt gtccccaagc ccgaaaaaat gctccgtttc ctgaagattt
     2041 ttccgtatcc atacccgggc gtctccagtc cagtccttgc tctcccggcc gaatccgccc
     2101 tcatccgtca gcaggggctc atccgtagca acatcgtaaa aatccacctt ttgctcctcc
     2161 gtgcgaatgc ggatgcggag cagggacgtc tcaacgacat gaaccccggc ctcatcatac
     2221 tcgcggattt ccgctcccgg cgcggaatat ccggggctta cggcataact cggctcgtct
     2281 tccggcaccg ctccggggaa atacgttacg cggacaatac cggcatcagc aagacacatg
     2341 ctgagagtta ttttggaaac cgtattcgtg tactcccatt tgacgagact cccccggaga
     2401 ggaggtgttt ttcgtctttc agggatgttg gaaacggagc acattgaagc cggacggaag
     2461 agaggatcaa aagcgttcag tccagactaa atcaatttca tgacgaacgc aagattgaac
     2521 taaagcagca cttttctttt cattattaga gtaaaattat tcatattaat ctagaccgaa
     2581 catgaaaaat ctggttttat tgcgttggtg tacaacacgg cacgagtccg tatccttccc
     2641 ggcaccgaca aaccacatca accgatgagc acaattcgag ttcttacatt aggatgggaa
     2701 ttcccgcccc tggtcaacgg ggggttgggc attgcctgcc tgggtctttc caaggcgctg
     2761 gcaaaaaaag tggatttaag ggtgatcgtt cccaaggccg acccttccgt catttttgac
     2821 ggattccagc tcaccggtct caataacgtc tcctatcggg aagtggagca agtggaccgg
     2881 aaatattcct atgacagctt tgccctggtg gagcgcgcgc ccattgaact ggacccctac
     2941 accaccgtgg aagggggatc cggcgtggtg cagttcacca aggaaggccg catcaccttc
     3001 tccaaaacgc atgaagccga ccttcagctg ttcaggaaca aggaagacct ttacgccgga
     3061 gacctggccc tgaaagtcat tcaattctca aaaatagcgg taaaagtcgc ccttcagcag
     3121 gattttgaca ttatccacgc ccatgactgg atgacctatc tggctggcgt ggaagtgaaa
     3181 aaagccacgg gcaagcccct ggtggtgcat ctgcacgctt cccagtttga ccgtgccgga
     3241 gcggatgccc gcggctggat ttacgacatt gaaaaattcg gcatggaaca ggcggatgcc
     3301 gttatcccgg tcagtaaata cacgggaacc atcgccagcg ggcactatgc catcgacccc
     3361 cataagatat ttcccattca caacggagcg gatccggtca aagtcttcaa agggaagaaa
     3421 aaatttccgg aaaaactcgt cctcttcctg ggccgcctga cggctcagaa aggcccggga
     3481 ttcttccttc agattgccgc caaggttctg gaacagacgg acgacgtann nnnnnnnnnn
     3541 nnncttcaag ggcgtgggcg acaaattcca cttcaccggt ttcctgaaca aggataaagt
     3601 caatgaactc ctctccatca cggacatcta ctgcatgcct tccgtatcgg agcccttcgg
     3661 cctttctgcg ctggaggcgg cccaattcaa cattcccgcc gtgatttcca agcagtccgg
     3721 cgtagcggaa gtcatgaagg gagccctgaa agctgacttc tgggacgtca acaaaatggc
     3781 ggagcatatc gttcatctct gcacggatga ggaactgtac cggaaagtag tggaacagag
     3841 cacggaggac atcaaggcct ccacttggga cgccgccgcg gacaaggtca tccgggttta
     3901 tgaacatgtg ctgaaccgct aaaaacgcct ttttacccta ctccataact ctcagccttc
     3961 ttcaccccgt ccatgagcaa catttccctc actttcgttg cgcatcagcc caatcggctg
     4021 attcattatg acttcttcaa aattggagaa catgcctttt atgaagatga tgatctgaat
     4081 gcccgcgtgc ttagcacggt ggcggaacgc tgttatttcc cggcaacccg gctgatgaaa
     4141 cagcttattg aactcaccgg gggcaaattc cgctttgggc tggcgctcag cggagtcatt
     4201 ctggaacagg ccctgtacca caggccggac ctgattgccg cattcaagga tctggcggaa
     4261 accggctgtg tggactttct ggcaatgcct tattacaatt ccctggcatc cgtttactcc
     4321 cctcaggaat ttgccgaaca gattgaagaa caccgggagc ttctcaaaaa actcttccat
     4381 caggaaacgg atgtgctgat gaataccggc atgctgtact ccaacgccat tgcggcgcag
     4441 gcggaaacgc tcggcttcaa gggcatcatg gcggacggca acccggccat gctcaaggga
     4501 tttaccagca atgaagtctt cctggctccg tgggtgtata acaccaccac tatcttccgc
     4561 aacagggaac tctccaatga cctggcaatc atgcggactg atccggaatg gtcggaatac
     4621 ccgctttccc ccaccacttt cgcggattgg ctgacgcacc agcagggaag cgtcactacc
     4681 ctgtccatgg actatgaaac gctgggggaa cgccagtctg acgctacagg cgtcttcgaa
     4741 ttctggcgta cgatgattct cgcctgcata gacgccggca accggttcat gactccgtcc
     4801 gaagtagcgc aggaaatcaa gcccgtttcc gtctgtgagt gcacccagga aatgacctgc
     4861 tccacttttg gaaccatgtc ccattggaat ggcaacgtca tgcaggatga agccctccgc
     4921 aaaatctacc gtctggaaaa accggtcaag gcagcccatg acaaggatct cacccacgtt
     4981 tggcgcaaac tgcagagcgc ggaccacttc cactacatgc agaaggaaaa ctccttcacg
     5041 ccgtatgcat ccgcctttga cgcgtacatt tactacatga acgctttggc ggatttgcaa
     5101 atacgcgtca aaagggaatc tggaaaaccg gttcagccgg atccggaaaa agcgtcctga
     5161 cctcttctct tccgaatact gcgaacccgg gtggattata gtctgccggg aacatcacgg
     5221 gacggttccc ggctgtaaaa actttgcggt tccggaatgc ggtcttcttt gcccccccct
     5281 cctttggctg ccataaaggg cctgagggat gtcacggaaa gctaatatcc caatgccaga
     5341 cgcttcaatt tgttgcgcag ggtaatcttc tgtgcggaag aaacgcgctc cacaaaaagg
     5401 atgccgttca aatggtcgca ttcatgttgc aggcatctgg ccagaaggcc gttgcagtca
     5461 atcgttattt ctcttccgtc aatcagaagc accgtagcct tgacaaaatc cggcctcacg
     5521 acggaagcac gtattttcat cacgctcagg cagccttcat gaaaaggatg catggggccg
     5581 taaggttcca gaacgggatt ggcaaacatc aagggcatga tgtcggaaag ctctttgtcc
     5641 tctccgttca ctttaagcca ggtgacggac tcttcctcct tcggaatatc aatcactacc
     5701 agttgaatgg gaatgctcac ctgcggagcc gccaggccaa tgccctctgc tgcgtacatg
     5761 gtttccagca tattttccgc cagggttttc aggctgtcgt caaaatgttc tacagggcgg
     5821 catttttcct tcaataccgg atttccatac tgtgctattt ccaataacat cgggaggggg
     5881 ataatatata tgttaaggcg cttaccgtgc gccgggcttg ttgatttcta ttctggcagg
     5941 aacctcacct gcgtcaatac ccacagacgt cagcgcatcc caaatgccga caatgatgcc
     6001 aaactccgct tttttatcca catcaagctg cagtttgacg cctggatttt cctttttcaa
     6061 ggcttccagg gcagcagcca gttcattcat ctccaccagc ctgccattca gggaaatggc
     6121 ggatgtcccc gtcacggcca gaacggcccg ctgttccgaa gacggagtcc cctccatgaa
     6181 ttccgctccg ggtacatcta tcttaagcag ggactgcggc ttcttccact gcgtatggac
     6241 aatgaaaaaa atcagcagaa tggtcaatat gtcaatcatg gggacgatgg gcacccccat
     6301 gctccttgct ttcttgcggt aaaactgcat ggccttgtca gcgttgggaa tgatggcagc
     6361 ggaaggaaac cagttctgtc agcaggactt ccatggaggc ggaaatacgc tccagcttgc
     6421 gggaataata aacgtgggca atcacggccg gagtggcgat ggcgagaccg gcaatcgtcg
     6481 tattcagagc ctgggcaatc ccctgagcaa tcatgccgtg actctcatcc attccgaaaa
     6541 cgctgaaaat ctgcaccagg ccgctggcgg ttcccagaat gccgaacatg ggtgcgatca
     6601 tcacaatcat gtccagcagg ggcagacccg cctgaagcgt tacaaactcc tcgcgggcct
     6661 tcacctggat catttccttc aaggaggctt catcctccag cggtgaattc aacgcatcca
     6721 gcaccaggcg gccctctacg gaatccgaat cctcagctat tctctccaat tcagtgcgct
     6781 ccacggcgcc gcgggtgtac tgctccacgg ccctggtcag cacggcgggg caaatgaggc
     6841 tcttcttcat attgaacatg cggtaaataa cggccgcaat aagaagaacg gaacaggctg
     6901 ccagcgggta catgaaaacg ccgcctgcct ggaagaatgt aatgcagtct ttgatcaagt
     6961 tcatcatcat atatctgtga atggtatttg ttgcaggatg gtaggttgtt aaaaattgaa
     7021 atcgaaatac atttccaaag aatcccccac aagcatactc cttacctccg ggggcatcgc
     7081 tggaagcctg gcgagtttga tggcgaggaa ggtaaaggac ttctgtacct cgctggcacc
     7141 cacgcgggaa gtctgcctca tagacgaaat tttgccattc ttgttaatca ggatgcggat
     7201 gcgcacagtt ccggtaacaa tcaggtcgcg gttcaaatcg cactggcggt accattgttc
     7261 cccgatagcc cggtaaacga tcgcctggta cgttcccatg ggcgtagctt ccacatccaa
     7321 tgtcggacgg cggccaaagc tgaatttgcc gacctgcttg gtcttccgct catgtgtctt
     7381 gaagccgggc tggctctccg cattgaacat gggatcatac aagggctgtt tctgaaccgg
     7441 ttttgtattg ggcaccaaat ccagattggg aagatttagg ggaggattgt ccgccccttt
     7501 cgcaatgctt ttggccaatt cctcaagcgg atccggcttc tccgaatctc cggggctggg
     7561 caacactacc gcatggggaa gctgcatggc cttctcctgt gcatgacgga tgttttgtcg
     7621 ggccgttttt tcctcttccg atttgcctgg ctctcccttc ttgacgggag aatgagccag
     7681 ggaaccggga tgaccggcag gcggcatgga tggttccagg ggctggtagg gaacattgga
     7741 agaagctccg ggggaggcgt ttttgttgcc gtcccgttca tggcttaaat ccccatcctg
     7801 cctttcctgg ttaaaaagga caatctctcc ggtttcccgt ttcacgccat cctgggcggg
     7861 catatccctg tcgccgtccg gatccttccg gccgccttcc gcccgcgtgt tcctgttgct
     7921 ctggaatttg ggcacttccg gcctggtggc gctttcctgc tcctcattgg tcttcacgac
     7981 ggcaggcaga tattccgcgg gctcttgtct ccggctctcc agttgaggtt ccactttctt
     8041 taccatgcgc gcgcttacag ccactttcct ccgcgccgct tcagccggac gtggggaagc
     8101 cagccattct tcaggaacca ggcagctccc cgccagcaaa aacaggtgtg ccagcacaga
     8161 cagcaaaagg catgtgcccc aggaaacccg gaacagtccg tttctccgcc gctgcctgaa
     8221 actcatggag gtaaagtttt cacggttcca tgacgaatgc aatgtagaaa ggagacatga
     8281 tgaaggacga ttccgtaatt tagtattgca caagaccatg tgcaggccca tacttgctag
     8341 cgaccaaaca catgccttcc tcctaaacgg caaggccctt ttcctatata ccatacacct
     8401 cgttaacacc catcctatat ggccaatctc aacaaagtct tcttaatggg caaccttacc
     8461 gctgatcccg aactgcgcta cactcccaaa ggaaccgccg ttacggacat ccgccttgcc
     8521 atcaaccgtt actacgcggg cgacaacagc gaacgccagg aagaaaccac tttcgtggac
     8581 gtcacccttt ggaaccgcca ggcggaagtt gccggcaact acctcagcaa gggccgcgga
     8641 gtctttgtgg aaggacgcct tcaactggat tcctgggaag ataaagcctc cgggcagaaa
     8701 cgcaccaagc tgcgcgtgat tggggaaaac atccagctct tcccccgcgg cggtgaaggc
     8761 gatatgggag gtgctccgcg ccagcagtac cagaatgctc cccgttccaa caactacgga
     8821 caatcccgcc cggcccagaa ctacaacccg ccccccatgc cccccaccaa tcagccgtcc
     8881 aacgacttcg gagacat
//
LOCUS       DAOMSU010000068         8850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_556_length_8850_cov_2.558272, whole genome shotgun sequence.
ACCESSION   DAOMSU010000068 DAOMSU010000000
VERSION     DAOMSU010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_556_length_8850_cov_2.558272"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            317..>1887
                     /locus_tag="PK077_04440"
     CDS             317..>1887
                     /locus_tag="PK077_04440"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HRN23579.1"
                     /translation="MPGMAGAITIYGSFKLVQTGDVLFENNVTDKNTAGAIYCGNNEG
                     KRFGGQWLLSADGGNIVFRGNLVKGSSGVFARALGIFAYAPENDYTGMSQPNGNLTMD
                     FRAQAGREIVFYDGIDIESITVAMPTLHINRIPSDWADYGGIPVEFGGTVRFSGALTE
                     SFLVRNDGESDGDYAERVEASRRVKLESNIIVEGGRLVLEYGMNLANESGEVWQGSSR
                     VEQDKPVFNLAGGVLEITSGSSISAQQVTVSGHGSGAILRVGAAPIGSDSWEGKTYEL
                     PSLSAVTIDMSHGFDWDLMPFSERGDSGININASGEFLLGGTIGIADTLDDYASAAWG
                     RDREFVIFNMDSSSKRPQGEMKGVISNTTQSSTVDSPYTYGGTWEYEWRDMDGDGEDD
                     QLLAIWKHEAGCRPDQVRPEQAGELALNSLWSSASNAASLGNTALAQLTPVRLNQRYA
                     RNVGGMGLGDFARQRSRGGVDGYDYDGGGYSVGMDSDFGGKSGIWGIAFGQMHGFSKS
                     QDFNGRITQDSLMGS"
     assembly_gap    1888..1897
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1898..2497
                     /locus_tag="PK077_04445"
     CDS             <1898..2497
                     /locus_tag="PK077_04445"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HRN23580.1"
                     /translation="RIFRSGERSLWTVKADLTWGMTDNCMESRFSNGMDAHGEWNNRT
                     WLVQTEVSRSIQYAGGWTVSPFLRMEFTHGTEASFLEDGSYARKFEGAVLRRLSIPAG
                     VSVERSGDWKGRPWTQVLRLSYVGDAIQDVPEASVYSIYSDIFWKARGVKPARHAVRV
                     EYDAALQWNDRWTVYAGYGMEARGSSVYHRVNAGVSMAF"
     gene            complement(2589..4460)
                     /locus_tag="PK077_04450"
     CDS             complement(2589..4460)
                     /locus_tag="PK077_04450"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRN23581.1"
                     /translation="MRSDMDEKRLAGVVLPLFSLRRNNDHGIGDLTALRQWIDWAADA
                     HVGFLQLLPVNALGRDECPSPYSAISSVALEPLYLSLEPWTIPGLEERVFNETGDTLP
                     WEQPSGPDLVDYPKVRFWKMWILRGAWNNFKTKPEYECIMPKFREWVKEQGSWLEDFV
                     CFQILCDLFGTEIWWHWPEQDPARAKAIASDYEEEKDFARWLQWLCEKQWEFIRIYAD
                     ERNVKLMGDIPIGVSLSSADVFFERHLFDTEWCGGAPAEGSYAEDPFTVKWGQNWGIP
                     LYRWDVMAQDNFAWWRRRVKYCTKIFSMYRIDHILGFYRIYSFPWKPTENGVFLPLST
                     DQAAQRTGGRLPGFKPRGDDNAADRNMNLADGDLYLRLLLSAAPGVSVVGEDLGCVPD
                     YVRPNMRQLDIPGFKIPHWEIKSDGTITSGKEYHECSFAAFGTHDFETIMQTWNDSYA
                     KIERAHKLGLWENGSPKTPSNPEQENIVRQAEDGARLLKWFADFSGMQPETCLSYWNQ
                     EIKTAMYNALFRSRSRYAAILWPALFDINKRLNIPGTTGGTNWRERMPFKAVEACGMP
                     QTAWLRTVIDESGRTPLQGEDAIRALKESSKRLFPKITVNNERFLKRMLTWPNGKTL"
     gene            4827..5438
                     /locus_tag="PK077_04455"
     CDS             4827..5438
                     /locus_tag="PK077_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23582.1"
                     /translation="MKLLFLPSALLVLLASCGPNKHVNAWDRQSSALDPVGITSKKMA
                     AKNKAEQAFYKPGEAVTFKKDRMMVFEQNPESNFSTSGTVRGGVKTGKVLVCGDLFAK
                     VEFEDGSQGYVSLSEIVNPQEMMVFYPVSASDLIGPDGALLPLPSNMGAVSPEAAAAS
                     RELDISRMNTSLVPVPNSVSPSGAASQEAPPENVPLPESSVKQ"
     gene            complement(5553..6911)
                     /gene="rimO"
                     /locus_tag="PK077_04460"
     CDS             complement(5553..6911)
                     /gene="rimO"
                     /locus_tag="PK077_04460"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRN23583.1"
                     /translation="MPLTVGLISLGCPKNLVDSEIMIGHLQKAGMTMTPEPELADVMV
                     VNTCAFIDQAKQEAIDAILDIVRARENGAYPENQKLIVAGCLSQRFRKELPALLPEVD
                     AFIGPDQITKLPEIITQVMDRTIQNRNFIEGKCRYVPDWNTPRYRLTPPHTAYIKIAE
                     GCNHGCAYCIIPMIRGRHRSRSQQDVVREAETLIRSGVKEICLIAQDITYYGMDKWTD
                     ARPNRRSAVDSSRGESLASLLRALNAIEGEFWIRLLYTHPAHWSDELTAAIAECPKVA
                     RYVDIPLQHISDNMLDAMQRVTDGDYIRTLLRNIRKAVPGIAIRTTFITGFPGETEDD
                     HQELMEFIEEFRFERAGIFTFSREEGTKAYKMPNQVHHRTKARRYNEATLLLARLASE
                     TGQEQIGRQIRVLVDAPGVARTEWDAPDIDGTVSVPLTLPVGQFATVTVTDAVAYELT
                     AE"
     gene            complement(6938..8374)
                     /locus_tag="PK077_04465"
     CDS             complement(6938..8374)
                     /locus_tag="PK077_04465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23584.1"
                     /translation="MKRIHLLLLALLALFAGAPFRAAAEETFRDRVVVIPVGEDSLTS
                     KQSFGFMNRILKRAQEEQARAVVFDLNTPGGLAWETSEMMMKSIQPLTIPTYAYVNPR
                     AMSAGALISAACDKIYMAPVSSIGAAGIITSSGEEMDPVMRKKAESAFWAFTRSVVTE
                     KGYRPEVVKAMMIPSDQDQQFGPVKLEKGALLTLTGKEAAARLEDGRPLLAQGTASSV
                     TDLLAQEKVNAPVVTAEPTAFERIGLWIAWASPILILLGIGGIYMEFKTPGFGIGGIV
                     AIAAFALFFFGNNIAGNLAGYETAALLVLGIVLLCVEFFVIPGTFIAAIAGGVCIFLA
                     LFGGMVSSWEWDHIIRDGQWDDVNTLAAVLGIPLLKLALGLTGGSLLIALLMKYLPDS
                     ILMRRVTNATVSGGSTGEAVNIARVQVGDRGNAVTELKPNGKAMIHGEICEVFSRQGI
                     LIKGTPVRVVDIRPFDLVVVKDDSPSGD"
     gene            8533..>8850
                     /locus_tag="PK077_04470"
     CDS             8533..>8850
                     /locus_tag="PK077_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHC2 zinc finger domain-containing protein"
                     /protein_id="HRN23585.1"
                     /translation="MIDWFRLMGRIDEDSIRRVLEATDIVDVVGSYLPLKRAGSRWKA
                     CCPFHNEKTPSFIVDQSRQNFKCFGCGEGGSAITFVMKMENLGFADTVRRLAEKAGIS
                     IVEE"
BASE COUNT         1849 a         2248 c         2683 g         2060 t
ORIGIN      
        1 taaccagggg ggggattctt ccctttactc caaacctgga gatgggaata tttcttttga
       61 ccatacggga aatattatct tcaggaatct tgttaaaact tcttattaca taagttctgc
      121 ggggattttt accaatgaag ggagtatttc atttaatgat acggaaaata tcctgtttga
      181 gaataacacg tccaccggag gacctgcggc gataggcatc ggatccatat tcttaccgga
      241 taaccagccg tctttgtcgt ttagcaacat ccggggagat attattttta gaaataataa
      301 agcttccgga gggagcatgc cggggatggc aggcgctatc actatttatg gatcttttaa
      361 gctggtgcaa acgggtgatg tcctgtttga aaataatgtg actgataaaa atacggccgg
      421 agccatttat tgcggcaata atgaagggaa aaggtttgga gggcaatggc ttttgtctgc
      481 ggatggcggg aatatcgttt tccgaggcaa cctggttaaa ggaagttcgg gggtctttgc
      541 ccgcgccctg ggtatttttg cttatgctcc cgaaaacgat tatacgggca tgtctcagcc
      601 caatgggaat ttaaccatgg atttccgcgc ccaggccgga cgagaaattg tattttatga
      661 tggtattgat attgaaagta ttactgtagc catgcctacc ctgcatatta acagaattcc
      721 gtccgattgg gcagattatg gaggtattcc tgtggagttt ggaggaaccg tgcgtttttc
      781 cggtgcattg acggagtcgt ttctagttcg caatgacggg gaaagtgatg gtgattacgc
      841 ggaacgggtg gaggcctccc gcagggtgaa acttgaatca aatattattg tggaaggggg
      901 gcggctggtt cttgaatacg gcatgaacct ggcgaatgag agtggggagg tatggcaggg
      961 aagttcaaga gtggagcagg acaaaccggt gtttaacctg gccggcggag tgctggaaat
     1021 aacgtccggt tcatccatca gcgcccagca ggtgactgtt tccggccatg gcagcggcgc
     1081 tatcctgcgg gtgggagccg ctcccatcgg ttccgacagt tgggagggga agacctatga
     1141 actgccttcc ctttccgctg tcaccattga catgtcccac gggtttgact gggatctcat
     1201 gcctttctcg gaacgggggg acagcggtat caacatcaac gcctccgggg aatttttact
     1261 ggggggaacc attggaattg cggatacgct ggatgattac gcctctgccg cctggggaag
     1321 ggacagggag tttgtcattt ttaatatgga ttcttccagc aaacgtccgc aaggagagat
     1381 gaaaggggtt atttccaata cgacccagtc atccacggtt gattcgccct atacctacgg
     1441 cggtacctgg gaatatgaat ggcgcgacat ggatggagat ggagaagatg accagctgtt
     1501 ggcaatttgg aaacatgaag ccggatgccg cccggatcag gtccgtccgg agcaggctgg
     1561 agaacttgcg ctcaattccc tttggagttc cgcgtcaaac gcggcttctc ttgggaatac
     1621 tgcgttggcg cagttaaccc cggtgcggtt gaatcagcgg tatgcacgca atgtaggggg
     1681 aatggggctg ggggatttcg cccgccagcg ttcccggggc ggcgtagatg gttatgacta
     1741 tgacgggggc ggatattccg ttggcatgga ttccgatttt ggaggaaaga gcgggatatg
     1801 ggggatagcg ttcggacaaa tgcacggttt cagcaagagc caggatttca atgggaggat
     1861 tacccaggat tccctaatgg ggagcatnnn nnnnnnncgg atattccgct ccggagagcg
     1921 ttccctgtgg acggtgaaag ccgatcttac ttggggaatg actgataact gcatggaaag
     1981 ccggttcagc aacggtatgg acgctcatgg ggaatggaac aacagaacgt ggctggtcca
     2041 gacggaagtt tccagaagca ttcagtatgc aggaggatgg actgtgtctc cgtttttgag
     2101 gatggagttt acacatggca cggaagcttc ttttcttgaa gacggcagct atgccagaaa
     2161 atttgaagga gcggttttgc gccggttgtc catcccggca ggtgtgagtg ttgagagaag
     2221 tggtgactgg aaaggcaggc cctggacgca ggtgctccgc ctttcctatg tgggagatgc
     2281 tattcaggat gtgccggagg cttccgttta cagcatatac agcgacattt tctggaaggc
     2341 ccgtggtgtc aaaccagccc gccatgccgt acgtgtggag tatgatgccg ccttgcagtg
     2401 gaatgaccgc tggacggttt atgccggtta tggcatggaa gcccggggaa gctccgtgta
     2461 ccaccgcgta aatgcaggcg tatccatggc gttttaggaa acgtcagcac ggatagaaaa
     2521 aagggcacgt ttccttcgaa agcgtgccct ttttgatact acgggggatg ttccggcaag
     2581 gggaacggct acaatgtttt gccgttcggc cacgtcagca tccgtttcag gaagcgttca
     2641 ttgttgacgg tgatcttcgg gaagagacgt ttgctggatt ccttgagcgc cctgatggcg
     2701 tcttcaccct gcagcggcgt gcggccggat tcgtcgatga cggtgcgcag ccatgcggtt
     2761 tgcggcatgc cgcatgcttc cacggccttg aagggcatgc gttccctcca gttggtccct
     2821 cccgtagtgc cgggaatatt gagccgtttg ttgatgtcga agagagcagg ccagagaatg
     2881 gccgcataac gggaacggga acggaacagg gcgttgtaca tggccgtctt gatttcctgg
     2941 ttccagtagg aaagacaggt ttcaggctgc atgccggaga aatccgcaaa ccatttcagc
     3001 aggcgcgctc cgtcctccgc ctggcgcacg atattttcct gttccgggtt ggacggcgtt
     3061 ttgggtgagc cgttctccca caggcccagc ttgtgggcgc gttcgatttt cgcgtagctg
     3121 tcgttccatg tctgcatgat ggtttcgaaa tcatgcgttc cgaacgccgc aaaggagcat
     3181 tcgtgatact cttttccgga cgtaatggtt ccgtccgact tgatttccca gtggggaatc
     3241 ttgaaaccgg ggatatccag ctggcgcatg ttcgggcgca cgtagtccgg aacgcagccc
     3301 aggtcttccc ccaccacgct gacgccggga gcggcggaaa gcagaaggcg caggtacagg
     3361 tccccatccg ccaggttcat gttgcggtcc gcagcattgt catccccgcg cggcttgaag
     3421 ccgggcagcc ttccgcccgt tctctgggcc gcctgatcgg tggaaagcgg caggaatacc
     3481 ccgttttccg tgggtttcca cgggaaggag taaatgcggt aaaagcccag gatgtggtcg
     3541 atcctgtaca tgctgaaaat cttggtgcag tattttaccc ggcggcgcca ccaggcgaag
     3601 ttgtcctgcg ccatgacatc ccatctatac agcggaatgc cccagttttg cccccatttg
     3661 accgtgaatg ggtcttccgc atagctgcct tccgccgggg ctcctccgca ccattccgtg
     3721 tcaaacaggt ggcgctcaaa aaagacgtcg gcgctggaaa gggaaacgcc aatggggata
     3781 tcccccatca gcttcacatt gcgttcgtcc gcataaatac ggatgaattc ccactgtttt
     3841 tcacagagcc actggagcca gcgggcaaaa tccttttcct cttcgtaatc ggaggcaatg
     3901 gccttggccc gcgccggatc ctgttccggc cagtgccacc agatttccgt accgaacagg
     3961 tcgcagagaa tctggaaaca gacaaagtct tccagccagc ttccctgttc tttcacccat
     4021 tccctgaatt tgggcataat gcactcatat tccggcttgg ttttgaaatt gttccaggct
     4081 ccccgcagaa tccacatttt ccagaatctg accttggggt agtccaccag atccgggccg
     4141 gagggctgtt cccacggcag cgtgtccccc gtttcattga atacgcgttc ttccaggccg
     4201 ggtatggtcc agggttccag ggaaaggtac aggggttcca aagctacgga gctgatggcg
     4261 gaataggggg aggggcattc gtccctgccc agggcattga cggggagcag ctggaggaac
     4321 cccacatggg catccgccgc ccagtcaatc cattggcgca aggccgtcag gtccccgatt
     4381 ccgtggtcat tgttacggcg cagggagaac aatggcagaa ctacgccggc taatcttttc
     4441 tcatccatat cagaacgcat tacttgagcg gttcccccgg atggacctgc gtcagccgtt
     4501 ggaacagtaa gctccagaat agcatttccg gcaggaaggc ggcaagctta atacgttgtc
     4561 tccgcgccat gctttacggt ttttcctggg aagtggagca gggggacggt aagacaggct
     4621 gttttttccc gccgcaggaa tagacgcctt accgggaatc cgattatttg tccgggatgc
     4681 agttcggaag cggcatggtt ttctctgcgg gaaacagcat gaaccggagg gaaaggcgga
     4741 atttttcttt tagaaacagt ctctggcgga aacaggcgca aattctgctt ccatccgtaa
     4801 tccccggcaa ttaagataat ctgcaaatga aattgctttt tctcccttcc gctttgctgg
     4861 tgctgctggc ctcctgcggt ccgaacaagc atgtgaatgc ctgggaccgt cagagttctg
     4921 cgttggatcc cgtgggaatc acttccaaga aaatggcggc taaaaacaag gcggagcagg
     4981 cattttacaa acctggggaa gccgttacgt tcaagaagga cagaatgatg gtgtttgaac
     5041 agaatccgga aagtaatttt tccacaagcg gaaccgtcag gggtggggtg aaaaccggga
     5101 aagtgctggt ctgcggggat ttgttcgcca aagtcgagtt tgaagacggc agccagggtt
     5161 acgtaagcct ctcggagatt gttaatccgc aggaaatgat ggttttttat cctgtttccg
     5221 cttctgattt aattggtccg gacggagctt tgcttccttt gccctcaaac atgggcgccg
     5281 tcagcccgga agccgccgcc gcttcccgtg agctggatat atcccggatg aatacctctc
     5341 tggttcctgt tccgaattcc gtttctccgt cgggagctgc gtctcaggaa gctcctccgg
     5401 aaaacgttcc cctgcctgaa tccagcgtga agcagtaggg cctttgcaga aaatccggaa
     5461 agtcgggcgg aaaacaggag gcaatatacg tcggcctgcg cttcgcctgt atttttgttc
     5521 ccgggttctt tccgacagga acggaagcaa ttttattccg ccgtcaattc atacgcaacg
     5581 gcatccgtta cggtgacagt agcgaattgc ccgacgggca gcgtcagagg aacggaaacg
     5641 gtcccgtcaa tgtccggagc atcccattcc gttctggcga ctccgggagc atccaccagc
     5701 acgcgtattt gcctgccgat ttgctcctgg cccgtttccg aagcgagacg cgccagcaac
     5761 agggtggctt cattgtagcg gcgagccttg gtgcggtggt gcacctgatt gggcatcttg
     5821 taggcttttg tcccttcctc ccgtgagaag gtgaaaatgc cggcgcgttc aaaacggaat
     5881 tcctcgataa actccatcag ttcctgatgg tcgtcttccg tttctccggg gaatccggtg
     5941 atgaaggtgg tgcggatggc gatgcccgga acggccttcc ggatattccg gagcaacgtg
     6001 cggatatagt ctccgtccgt tacgcgctgc atggcgtcca gcatattatc ggaaatatgc
     6061 tggaggggaa tgtctacata gcgggccact ttggggcatt ccgcgatggc tgcggtcagt
     6121 tcgtcgctcc agtgggcggg gtgggtgtag agcagcctga tccagaattc tccctcaatg
     6181 gcgttcagag cgcggagcag ggaagccagg gattctccgc gggaggaatc caccgcgctg
     6241 cggcggtttg ggcgcgcatc cgtccattta tccatgccgt aataggtgat gtcctgcgcg
     6301 atgaggcaga tttccttcac gccggaccgg ataagggttt ccgcttcacg cactacgtcc
     6361 tgctgggaac ggctgcggtg gcgtccgcgg atcatgggaa tgatgcagta ggcgcagccg
     6421 tggttgcagc cttccgctat cttgatgtag gccgtgtgcg gcggggtaag gcggtagcgc
     6481 ggtgtgttcc agtcaggaac gtacctgcat tttccttcaa tgaagttccg gttctgtatg
     6541 gtccggtcca tcacttgagt gataatttcc ggcagtttgg tgatttggtc cggaccgata
     6601 aaggcgtcca cctccggaag cagggcgggc agttccttcc ggaagcgctg ggacaggcag
     6661 cccgccacga tgagtttctg gttttccgga taagccccgt tttcccgggc acgcacgatg
     6721 tccagaatgg cgtcgatggc ttcctgcttg gcctggtcaa tgaaagcgca ggtattgacc
     6781 accattacgt ccgcgagttc cggttccgga gtcatggtca tgccggcttt ttgcaggtgg
     6841 ccgatcatga tttcagagtc aaccagattc ttgggacagc ccagggaaat gagtccaacg
     6901 gtgagaggca tggcagtaga taagaagcgt gatggcgtca atccccggag ggggagtcgt
     6961 ccttgacgac gacgaggtcg aatggccgga tgtccacgac gcgcacaggg gtacccttaa
     7021 tcagtatgcc ttgtcgagag aaaacctcgc atatttcgcc atgaatcatg gcttttccat
     7081 tgggttttag ctccgtgacg gcgttcccgc ggtctcctac ctgaacgcgc gcgatattga
     7141 ccgcttcccc ggtggaacct ccgctgaccg tggcgttggt aacgcggcgc atcaggatgg
     7201 aatccgggag gtatttcatc agcagggcaa tcagaaggga gcctccagtc aggcccaggg
     7261 ccagtttcag aagggggatg cccaggactg ccgccagcgt gttgacgtca tcccactgtc
     7321 cgtcccggat gatatggtcc cattcccagg agctgaccat gccgccgaac agggccagaa
     7381 agatgcagac gccgcccgca atggccgcaa tgaatgtgcc cgggataacg aagaattcca
     7441 cgcaaagaag gactatgccc agaacgagca gggccgccgt ctcatagccg gccagattcc
     7501 cggcaatgtt gttgccgaag aagaacaggg caaacgcggc aatggccaca atgccgccga
     7561 tgccgaatcc cggcgtttta aattccatgt aaatgccgcc gatgcccagc aggatcagga
     7621 tgggggaagc ccatgcaatc cagaggccga tgcgctcaaa ggccgtaggt tccgccgtga
     7681 ccacgggggc atttactttc tcctgggcca gcaggtccgt gacggaggag gctgtgccct
     7741 gggccagcag gggcctgccg tcttccagac gcgcggcagc ttccttgccc gtcagggtca
     7801 ggagcgcgcc tttttccagt ttgacggggc cgaactgctg gtcctggtcg gacgggatca
     7861 tcatggcttt caccacttcc gggcggtaac ccttttccgt cacgacggag cgggtaaagg
     7921 cccagaaggc gctttccgct tttttgcgca tgacggggtc catttcctct ccggatgagg
     7981 tgatgatgcc cgctgcgccg atggaggaga cgggagccat gtagattttg tcgcacgccg
     8041 cggagatgag cgccccggcg gacatggccc tggggtttac gtaggcgtag gtgggaatgg
     8101 tgagcggctg gatgcttttc atcatcattt cgctggtttc ccaggccagg cccccgggcg
     8161 tgttcaagtc gaatactact gcgcgagcct gctcctcttg cgctcttttc aggatgcggt
     8221 tcatgaagcc gaagctttgt ttgctggtca gggagtcctc ccctacgggg atgacgacta
     8281 ccctgtcgcg gaaggtttcc tccgcggctg cccggaaggg ggcgccggca aacagggcca
     8341 gcaaagccag caacagaagg tgaatgcgct tcatacgggc aggctagcat gttccgctcc
     8401 cgcgctcaat ggggaaatgt gttcctcagg ggcgggaagc cgccggtgct gcgccgtctt
     8461 ttcccagcct gtggaggcct tgtttcctcg catggcgttc cgattctgta ttcttgtgca
     8521 cccgtacccc ttgtgataga ctggttccgc cttatgggaa ggattgatga agacagtatc
     8581 cggcgcgttc tggaggccac tgacattgta gatgtggtgg gatcctatct tcccctgaaa
     8641 agggctggtt cccggtggaa ggcatgctgc ccgttccaca atgaaaagac gccttccttc
     8701 attgtggacc agagtcggca gaattttaaa tgctttggct gcggggaagg ggggtctgcc
     8761 atcacttttg tgatgaagat ggagaacctg gggtttgcgg ataccgtgcg aaggctggcg
     8821 gaaaaagccg gaatctccat tgtggaggag
//
LOCUS       DAOMSU010000069         8850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_557_length_8850_cov_2.210460, whole genome shotgun sequence.
ACCESSION   DAOMSU010000069 DAOMSU010000000
VERSION     DAOMSU010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8850
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_557_length_8850_cov_2.210460"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    354..363
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <364..765
                     /locus_tag="PK077_04475"
     CDS             <364..765
                     /locus_tag="PK077_04475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN23586.1"
                     /translation="YAYAGVFKDRAAYRWAVETTVYVREDMKKMGTGRELYAALEKIL
                     SMQNILNLNACIAYPCEAEDPYLTRDSVQFHKRLGYQFAGKFHRCGYKFGRWYHMIWM
                     EKFLGGHTENPPVVKSFGEVRSLVAEKLGIW"
     gene            1020..>2117
                     /locus_tag="PK077_04480"
     CDS             1020..>2117
                     /locus_tag="PK077_04480"
                     /inference="COORDINATES: protein motif:HMM:NF020932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HRN23587.1"
                     /translation="MNAYKRMLLAGFCAGSTILAFGSQAQAAKAASPKVDKTSAAIAA
                     YADELVIPTYKMMSDNALKFAKAAKELKAAPTDAKVAEAGKLLLETRVPWELSESFLF
                     GPAAFANLDPKLDSWPLDTTNLDAVAKNADSKSVTIDAAYVRNSLGAETRGFHAAEYL
                     LFRDGQPRKAADLTPGQLSYLAAVAEVIAEDSITLEAWWAGSDKISEEKAKILEEAEI
                     EPGKSYAGEFKKAGQAGSRYESNSEVLDEIIGGSKDIIDEIADSKVGKPYETADAADC
                     ESLYSYTSLVDSRHNVQSVEKSYNVISPLVAAKSAKVDQAVKGSIAKVFKSLDAIQGP
                     LVKNLDKKEQLKAIIDSCKELSENLDKVQELL"
     assembly_gap    2118..2127
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2491..3909
                     /locus_tag="PK077_04485"
     CDS             2491..3909
                     /locus_tag="PK077_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HRN23588.1"
                     /translation="MTASCVSCHVNRGRDFTPPALRTKDGDPGPAYYLGGEKGTVFNA
                     TSKAFEQESPAITEAGLTNRFKAGEIIFEGNFVPVKRNRFGGLGPTYIKSSCLACHPG
                     YGRGQRTGNFDRQYGNGYLAFVHNPDGTPVKGYTGMLQTKAVPPFVPYAKGVKIEWHD
                     FVDQYGNKYPDGTPYNAGKPTEGTLTYPTADVIEPLLPLPSDYRVSIESTIGIYGTGL
                     LDAIRDEDIIAEYRRQQSMTGPVKGVPGKWIDEPDGTRRLGKFTWDCSRATLENGPGA
                     NALWNVTNVTRKNRPNIYMTPEWLEKQKELGIDVSDLEGPQEEELSMQQYEDFMVWHR
                     GLAVPAARNLDKPDVRRGQELFNKLGCAGCHKPEWTTGEYKPLPGYANQTIRPYTDML
                     RHDMGEINRGRSRFWRTPPLWGRGLMHKTANHTDMFHDLRARDFEEAILWHFGESEFS
                     REMFRHLSAKERGQLIQFLKSL"
     gene            3926..5014
                     /locus_tag="PK077_04490"
     CDS             3926..5014
                     /locus_tag="PK077_04490"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRN23589.1"
                     /translation="MTCNLEKNKGVSVKSLPLGGEGASLQFVTLAGSEPGQLAAHAEL
                     LQKAEKNGLRWIGPEVYRSADLDISPVPGAGMTAFLGTKTGASFYRGMQICAVEGVNA
                     RAIEFNGRSAGVFYEDEYAEYAVLGGLSPRDRTAGRTEQTLDVFRQMEEALNRAGMDF
                     SHVVRTWFYNNRLLDWYEEFNRARDTFFESRGVFDRLVPASTGIGSGNRENAALSARV
                     FAIRPKGDRVTVKDIPSPLQCSAQDYRSSFSRAVEVDHPLFRQIIVSGTASISPGGES
                     VHLGDIDKQTELSLDVIEAILKSRGMGWKDVVRSVAYFKDKDYASHYEKIALRRGLAI
                     MPCIGIQADVCRDNLLFEMELDAAVLKA"
     gene            5019..5984
                     /locus_tag="PK077_04495"
     CDS             5019..5984
                     /locus_tag="PK077_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRN23590.1"
                     /translation="MAFRIIQWSFLLGLAFYSLFIGAAQVSLSDLWRMDGEQLQFWWE
                     IMLQSRIPRLCSILVVGSSLAISGLIMQRITNNRFVSPTTAGTMEWCRFGVMIAILYL
                     PGASPIWRLGAAFVIALIGTSCFLAVIARVRHQNVILVPLIGIMLGGVVNAGAVFYAY
                     QFDIVQNMASWLQGSFSLVIEGNYELLYASIPLMLLAYVYADLFTIAGMGRDAAVALG
                     LNHASIMHMGLVIVSIISSITIVEVGYLPFIGLIVPNIVSIFRGDAVKKILFDTAWLG
                     AMLVLVCDVAGRMLIAPFEIPIGVILSVVGGAVFLAMLFHRKTYA"
     gene            5977..>6329
                     /locus_tag="PK077_04500"
     CDS             5977..>6329
                     /locus_tag="PK077_04500"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRN23591.1"
                     /translation="MPDSIQHQKDDAFRNAAGRRRAFPAFLILGLLCVGLALVYVFQG
                     MTPETWDFNMARRIPIVIALVLVGTAVGLSSVVFQTITTNYILTPSVMGLDNLYVLLQ
                     TLVLYFVGSTQLTTM"
     assembly_gap    6330..6364
                     /estimated_length=35
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6365..6961
                     /locus_tag="PK077_04505"
     CDS             <6365..6961
                     /locus_tag="PK077_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HRN23592.1"
                     /translation="FYMFRGQNGGNIYFVVLVGMIFGITFGGLSNFMQVLIDPSEFAI
                     LEGRLFASFNRINEELLFTAGLVIAATVIWLVCDLRKLDVLTLGRSTAITLGVNYKWV
                     VLRSLMIVSILASASTVLVGPVTFLGILIVSIARFIFPTYRHIVLMPGTALVGVAALT
                     FGMLLTERWLNFSVPLSVIINFVGGVYFIYLIMKIKRI"
     gene            6958..7716
                     /locus_tag="PK077_04510"
     CDS             6958..7716
                     /locus_tag="PK077_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN23593.1"
                     /translation="MIEIRDISTKYRETFVLRHVSTRLPSDRMVAFIGSNGAGKSTML
                     NIISRLLEPTEGSVSIDGRELRKWDSRELAKTLTILGQTLHTPARLTVEELVRFGRFP
                     HSRGRLENQDFLQIEKALELTEIVDLRHCYLDELSGGQRQMAYIAMAIAQDTKYIFLD
                     EPLNNLDMHRAARIMKLLRSLVREQGKTICIVIHDINFVSFYADYVVAFRDGVLCHQG
                     PTEDIIRTDVLRDIYGMDIAIEPYGDKKICVYYE"
     gene            7759..8682
                     /locus_tag="PK077_04515"
     CDS             7759..8682
                     /locus_tag="PK077_04515"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HRN23594.1"
                     /translation="MRTTITAFLAALILPGLSMAAADEAKTITATHTLGTVELPVNPK
                     RVAVLDYGSLETISELGVTPALALPKKFLPPYLSKFSGEQYTDLGTVKEFNIETINAF
                     KPDLIIISGRQQDYYQKLSSIAPVYLVDILAKDQLGEAKKNIRLLGDVFGVPEKAAEA
                     VKNIDEAVTRTKDKAQASGKKALVLLTNDGKVSAYGSGSRFGLVHDALGVAQADKNIK
                     VGVHGQQVTYEYVAMLNPDIIFVVDRSVAIGRSAKNPNVLNNVLVNKTKAAKNGAIIL
                     LDPQVWYLSGGGIISLNKMISEVEAAFTDKK"
BASE COUNT         2035 a         2287 c         2411 g         2062 t
ORIGIN      
        1 gaaatgaata ttgaccccgt ttcttcccgt tcatgaacgg gaacggcgga aaacatgcgg
       61 cggatgatat ttcccggaaa tggcgggtgc ttgggcggcc tcaaattgac aatcgcttgt
      121 gaaatattag aatgatcggc agcgcttccg gctcaggggc tcagcacggt caaccgaaat
      181 gaaaagcaac agaatcagaa ttgcaacgag gcgggatgcg gccaagctgc tggaaattta
      241 cgctccgtat gtggaaaaga cagctgtcac gtttgaatat gaagtgccag cagtgacgga
      301 gtttgagaaa agaatagatc acgtactgga aaaatacccc tatttggttg cggnnnnnnn
      361 nnntatgcct atgcaggagt gttcaaggac cgcgcagcgt atcgctgggc tgtggaaacg
      421 acggtatacg tgcgggaaga catgaaaaaa atgggaacgg gaagggaact gtatgcagct
      481 ctggaaaaga ttctctccat gcagaatatt ttgaatttaa acgcgtgcat tgcttatccc
      541 tgtgaagctg aggaccccta tcttacacgg gacagcgtcc agttccataa acgtttgggg
      601 tatcaattcg cagggaaatt tcatcggtgc ggatacaaat tcggccgctg gtaccatatg
      661 atctggatgg agaagtttct tggaggccat acggaaaatc cgcccgtagt gaaatctttt
      721 ggcgaggtaa ggagccttgt cgcggagaaa ctcggaattt ggtgaaaggc ggctcctggg
      781 cgcattcgga aacagaaaat atttccgaat caataacatg ccgatgttcc ggcatgcggt
      841 cacaaggggg actttttgtt gcaaagttag tttggactaa tttttaattg acatcgtttg
      901 gccggattat tattttccgc tccggcggga aaaaatggcg gatacgccat gaaatcctgc
      961 cggattggaa gatgcctccc tgaggaggat acggatgttt tcattatcaa taaaaaacca
     1021 tgaacgcata caaacgcatg ttgttggccg gcttttgcgc cggaagtaca atcctggctt
     1081 tcggtagcca ggctcaggcg gccaaggccg cttcccccaa ggtggataaa acgagcgcgg
     1141 cgattgccgc ctacgcggat gagcttgtca ttcccaccta caagatgatg agtgataacg
     1201 ctctcaaatt cgccaaggcc gctaaagagc tgaaggctgc tcctaccgat gccaaggttg
     1261 ctgaagcagg gaagcttctt cttgaaacgc gcgtgccgtg ggaactttcg gaatccttcc
     1321 tctttggccc ggctgctttc gccaaccttg acccgaagct ggactcctgg cccctggata
     1381 ccacgaatct tgatgccgtc gccaaaaacg cggacagcaa gagcgtaacg attgacgccg
     1441 cctacgtacg caattccctc ggcgcggaaa cgcgcggttt ccatgctgcc gaatacctgc
     1501 tgttccgaga tgggcagccc cgcaaggccg ccgatctgac gcccggacag ctttcctacc
     1561 ttgccgctgt ggccgaggtg attgctgagg attccatcac gcttgaagcc tggtgggcgg
     1621 gttctgacaa gatcagcgaa gagaaggcca agattcttga agaagctgaa atagaacccg
     1681 gcaagtccta tgcgggggaa ttcaaaaaag ctggtcaggc aggcagccgc tacgagtcaa
     1741 actcggaagt gcttgatgaa atcatcggcg gcagcaagga cattattgac gaaatagccg
     1801 attcaaaggt gggcaagccc tacgaaacgg ctgacgccgc cgactgtgaa tccctttaca
     1861 gctacacttc tctggtggac tctcgccaca acgtacagag cgtagaaaaa tcctacaatg
     1921 tgatttcccc tctcgtagcc gccaagtccg cgaaggttga ccaggctgtg aaaggttcta
     1981 tcgccaaggt attcaagagc cttgacgcca tacaggggcc cctggtgaaa aatctcgaca
     2041 aaaaggagca gctcaaggca atcatcgaca gttgcaagga actttccgaa aacctcgaca
     2101 aggttcagga acttcttnnn nnnnnnngtg aagtaattta acccgcattg cccagggtcg
     2161 gcaaggccct gggctgcaat atcatattgt cggccggctg acggagacgt gtaccacggg
     2221 ccgtatttct ggaaagcctg ccaatcatca atgcacatgg agtatcgaca atttgcaaaa
     2281 cctgcggctg tcctggtgct cggagcggga gcggccgcct gggcaatagt gaattccgga
     2341 ggttctgggg atgcggctcc tgaacaatct tttatcccgg acacggcaag gaatgccggc
     2401 gttttgggac atccccgcat ttccggacgc gcactgatta atccggccca ggggagtgca
     2461 tcaaaatttg acaatatcaa agccgaatac atgacagctt catgcgtgag ctgccatgtg
     2521 aaccgcggcc gtgatttcac gccccccgcg ctcaggacga aagatggcga tcccggaccg
     2581 gcttactatc tgggcggtga aaagggaacg gtgtttaatg ccaccagtaa agcgtttgag
     2641 caggaatcgc cggcaattac tgaagccggc ctgacgaatc gtttcaaggc gggcgaaatt
     2701 atttttgaag ggaacttcgt acccgtgaag cgcaatcgct tcggcggtct cggccccact
     2761 tacattaaat catcctgtct ggcctgccac cccggttatg gccgcggaca gcgcaccggc
     2821 aattttgaca ggcagtacgg caacggttat ctggcctttg tacataatcc cgatggcacc
     2881 ccggttaagg ggtacacggg catgctgcag acgaaagcgg ttcctccttt tgtaccttat
     2941 gccaagggtg tgaaaataga atggcatgat tttgttgacc agtacggaaa caaatatccg
     3001 gacggaacgc cctacaatgc gggcaagccg acggagggca cgttgactta tcccacggca
     3061 gatgtgattg agccgttgct tccgcttccg tccgattacc gcgtgtcaat cgaatcaaca
     3121 atcggcattt acggaacggg gctgcttgat gccatccgtg acgaggatat tattgccgaa
     3181 tacaggcgcc agcagagcat gacaggcccg gtgaaggggg ttcctggcaa atggattgac
     3241 gagcccgacg gcacccggcg cctcggcaag ttcacgtggg actgctcgcg cgccacactg
     3301 gaaaacggtc ctggcgccaa tgcgctttgg aacgtgacga atgtaacgcg caaaaaccgt
     3361 ccgaacatct acatgacgcc cgaatggctc gaaaaacaga aggaacttgg cattgatgta
     3421 agcgatcttg aaggcccgca ggaagaggaa ctctcaatgc agcagtatga agatttcatg
     3481 gtctggcatc gcgggctggc cgtgcctgcc gcccgcaacc tggacaagcc tgacgtgcgc
     3541 cgcggacagg aactcttcaa taaactgggg tgtgccggtt gccacaagcc tgaatggaca
     3601 acgggggaat ataagccgct tcccggttat gcaaaccaga ccatccgccc ctatacggat
     3661 atgctgcgtc acgatatggg ggaaatcaac cgcggacgtt ctcgtttctg gcgtacgccg
     3721 cccctttggg gaagggggct gatgcacaaa accgccaacc ataccgatat gttccatgat
     3781 ctgcgcgccc gcgactttga agaggctatc ctgtggcatt tcggcgaaag cgaattctcc
     3841 cgtgaaatgt tccgccatct ctccgccaaa gagcgcggcc aactgattca attcctgaaa
     3901 tcactttaaa aaaactaata tactcatgac ctgcaacttg gaaaaaaaca aaggtgtttc
     3961 cgtcaagtcg ctcccgctgg gcggagaagg cgcatctctg caattcgtta cgctcgccgg
     4021 aagcgaacct gggcagttgg ccgcgcatgc cgaattactc caaaaagctg aaaaaaacgg
     4081 tctccggtgg attggcccgg aagtgtatcg cagcgctgat ctggatatct ccccggtgcc
     4141 cggggcgggg atgactgcct ttttgggtac gaaaacgggc gcatccttct atcgaggtat
     4201 gcaaatttgc gcggtggagg gggtaaatgc ccgggcgatt gagttcaatg gccgctccgc
     4261 cggcgttttt tatgaggatg aatatgccga gtatgccgtt ttgggcggtt tgtcgccccg
     4321 tgaccggaca gccggacgaa ccgaacagac attggatgta ttccggcaga tggaagaagc
     4381 gctgaaccgg gccggtatgg atttttccca tgtggtccgc acatggtttt acaataaccg
     4441 cctgctggac tggtatgaag agttcaaccg cgcccgcgac acattttttg aaagccgcgg
     4501 cgtgtttgat cgtctggttc cggccagtac ggggattggt tccggcaatc gggaaaatgc
     4561 ggccttgtcg gcccgcgtat ttgccattcg gcctaagggc gaccgtgtga cagtaaaaga
     4621 tatcccttcg cctctgcaat gttcggctca ggattaccgc agctcattca gccgtgcggt
     4681 agaagtggac caccctctct tccggcaaat catcgtctcc ggcacggcca gcatttcccc
     4741 tggcggagaa tccgtacatc tcggcgatat cgacaaacaa acagagcttt cgctggacgt
     4801 gattgaagcc atcctgaaat cacgcggcat gggctggaag gatgttgtcc gttccgtagc
     4861 ctatttcaaa gacaaggatt atgcctctca ctacgaaaaa atagctctgc gccgcggcct
     4921 cgccatcatg ccctgcatcg gcatacaggc cgatgtgtgc cgggacaatc tgttgtttga
     4981 aatggaactt gatgcagccg tgttgaaagc ctgagaaaat ggcattcaga atcattcagt
     5041 ggagctttct gcttggcctt gccttttatt cgctctttat cggggcggct caagtgtcct
     5101 tgtccgacct gtggcggatg gatggcgagc agctgcagtt ctggtgggag atcatgctgc
     5161 aaagccgcat tccgcgcctc tgcagcattc tcgtggtagg cagcagcctg gccatcagcg
     5221 gcttgatcat gcagcgcatc accaataacc gctttgtgtc gccgactacg gcaggcacga
     5281 tggagtggtg ccgctttggg gtaatgatcg ccattcttta tctgcccgga gcctctccca
     5341 tttggcgctt gggggccgcc tttgtgattg ctctgatagg cacctcatgc tttctggcgg
     5401 tgattgcgcg tgtgcggcac cagaatgtca ttctggtgcc cctgataggc atcatgctgg
     5461 gcggcgtggt gaatgccgga gccgtttttt atgcctacca gtttgatatt gtacagaata
     5521 tggcgtcatg gctgcagggg agtttctccc ttgtcattga gggtaattat gaattgctct
     5581 atgccagcat cccgttgatg ctgctggcct atgtttatgc cgatctcttt acgattgccg
     5641 gcatggggcg cgatgccgcg gtggcgttgg ggctgaacca cgcaagcatc atgcacatgg
     5701 gactggtgat tgtatccatc atcagctcaa tcacgattgt ggaggtaggc tacctcccct
     5761 tcatcggatt gatcgtgccc aatatcgtga gcattttccg gggggatgcc gtgaagaaaa
     5821 tcttgtttga tacggcttgg ctgggggcca tgcttgttct tgtttgcgac gtggcagggc
     5881 gcatgcttat cgcccccttc gaaatcccca tcggcgttat cctgagcgtg gtcggcggtg
     5941 ctgtatttct ggccatgctc ttccatagaa aaacgtatgc ctgattccat tcaacatcaa
     6001 aaagatgatg ccttccgcaa tgctgccggc cggcgtcgcg cgttcccggc cttcctgatt
     6061 ctgggtctgc tctgcgtggg gctggcgctc gtatatgtct ttcaggggat gacccccgaa
     6121 acgtgggact ttaacatggc ccgccgcatt cctattgtta ttgcgctggt gctggtagga
     6181 acggccgtgg ggctgtcttc agtcgtcttc cagacgatta caaccaacta tatcctcacg
     6241 cccagcgtaa tggggctgga taacctctac gtattactgc agacgctcgt gctctacttt
     6301 gtgggcagca cacagttgac gaccatgcan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6361 nnnnttctac atgttccgtg gacagaatgg cggcaatatt tattttgtgg tgttggtagg
     6421 catgatcttc ggcataacct tcggaggctt gtcgaacttc atgcaggtgc tgatagatcc
     6481 gagtgaattt gccatacttg aggggcgtct tttcgccagt ttcaatcgca ttaatgaaga
     6541 actgctgttt acggcggggc ttgtgatcgc cgcgacggtc atctggctgg tttgcgacct
     6601 caggaagctc gatgtgctta cgctgggccg ctccacggcc attacgttgg gcgtgaacta
     6661 caaatgggtg gtgctgcgct ccctgatgat cgtctctatt ctggcctcgg cctcgacggt
     6721 gctcgtaggg ccggtgactt tcctgggcat tctcatcgtg agcattgccc gcttcatatt
     6781 cccgacctac cgccacattg tcctcatgcc cggcacggct ctcgtgggcg tagctgcatt
     6841 gactttcggc atgctgctta ccgaacggtg gctcaacttc tccgtgcccc tgagcgtaat
     6901 catcaatttc gttggcgggg tttactttat ctacctgatc atgaaaatta aacgtatatg
     6961 attgagatac gagacatttc cacaaaatac cgtgaaacct tcgtgctcag gcacgtcagc
     7021 acgaggcttc cctctgatcg gatggtggcc tttatcggca gcaatggcgc cggaaagagc
     7081 acgatgctca acattatcag ccgccttctg gaacccacag aaggctctgt atccattgat
     7141 ggcagggaac tcaggaaatg ggattcccgg gagctagcca aaaccctcac catcctgggg
     7201 caaaccctcc atactccggc gcggctcacg gtcgaggaac tggttcgctt cggacgtttc
     7261 cctcattccc gcggccgcct ggaaaatcag gattttctgc aaatcgaaaa ggctttggaa
     7321 cttaccgaga ttgtcgattt gcgccattgc taccttgatg aactttcggg cggccagcgg
     7381 cagatggcct atattgccat ggccattgca caggacacga agtacatctt tctcgatgaa
     7441 cctttgaaca accttgatat gcatcgtgcg gcgcgcatta tgaaactgct gcgttccctg
     7501 gtgcgggagc agggcaagac gatctgcatt gtaattcatg atattaactt tgtctctttc
     7561 tatgcggatt acgtggtggc attcagggac ggagtcctgt gccatcaagg gccgacagaa
     7621 gatatcatcc ggacagatgt gctgcgtgat atctacggta tggatatcgc catagaaccc
     7681 tatggtgata aaaagatatg cgtatactat gaatagaatt tccttaaatt cccaaaacaa
     7741 caaacaataa caaataatat gagaacaaca atcaccgcat ttctggccgc attgattctt
     7801 cccggtcttt ccatggccgc ggcagatgaa gccaaaacga tcaccgccac gcacacgctg
     7861 ggcacggtcg agcttccggt caatccgaaa cgtgtcgccg tgctggacta cggttcgctt
     7921 gagaccatca gcgaactggg cgtcactccc gcgctggctt tacccaaaaa attccttcct
     7981 ccttacctct ccaaattctc cggagaacaa tatacggatc ttggaacggt caaggaattc
     8041 aatattgaaa ccatcaatgc gttcaagccg gacttgatta ttatttcagg acgtcagcag
     8101 gattattatc agaaactttc ctccatcgcc cccgtttacc tggtcgatat tctggccaag
     8161 gatcagctgg gggaagccaa gaaaaacatc aggcttctgg gtgatgtatt cggcgtgccc
     8221 gaaaaggcag ccgaagccgt caaaaacatt gatgaagccg taacccgtac gaaagacaag
     8281 gcccaggcca gcggcaagaa agctctcgtt ctcctcacga acgacggcaa ggtaagcgct
     8341 tacggttcgg gttcccgctt cgggttggtg cacgatgccc tgggggtggc ccaggccgat
     8401 aaaaacatca aggtgggggt tcatgggcag caggtgactt atgaatacgt tgccatgctc
     8461 aatcccgata tcatctttgt ggtggaccgt tcggttgcta tcggccgttc ggccaaaaat
     8521 ccgaatgtcc tcaacaatgt gcttgtgaac aaaacgaagg cggctaaaaa cggcgccatc
     8581 atcctgcttg acccgcaggt atggtatctt tccggcggcg gcatcatttc tctgaacaag
     8641 atgatctctg aagtggaagc tgccttcacg gacaaaaaat aattattccg tccttattca
     8701 tcacgaagca ggccgcacga ccctatgctt ccgtttcccg gctggagcgg cagaggcgtg
     8761 cggccctttt tcattcaggt cctgctgatg aagaaaattt tgcagtggat ggcatctccc
     8821 cgtcttgcgt gtgcgctgat ggcctacgcc
//
LOCUS       DAOMSU010000070         8845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_559_length_8845_cov_2.408419, whole genome shotgun sequence.
ACCESSION   DAOMSU010000070 DAOMSU010000000
VERSION     DAOMSU010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8845
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_559_length_8845_cov_2.408419"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..839
                     /locus_tag="PK077_04520"
     CDS             39..839
                     /locus_tag="PK077_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRN23595.1"
                     /translation="MNSPAPPCTRDHICWGAHASHPDRHRLEVDSLSVYYGSLLALNG
                     ISFSITCGHTLALMGPNGAGKSTLIKALAGLIRPDSGKILWNGRPLHDTPGEIAYLPQ
                     RSDVDWSFPITVRALVEMGRYPSLGLWKKFGRHDRDIVEKSLHALGMESLADRQISEL
                     SGGQQQRAFLARALAQEAHVLLLDEPFTGLDAPASQSLGRLLDSLAAEGRLVIASHHD
                     LNTAADIFDAILLMNRELAAFGPPKEVLSPARIREIYGMDPQPETQAS"
     gene            836..1699
                     /locus_tag="PK077_04525"
     CDS             836..1699
                     /locus_tag="PK077_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRN23596.1"
                     /translation="MMDDFLQFLREPIAQRSLLACVMIGFANGFVSGLVILKKSALQI
                     GTLSCALLPGIAVAVLLFGLTRWSLLTGAVVAALLVGLGSLFVSRTSRLNQDTALSIL
                     HTTAFAAGFIVLVRLGLQQKIDDWLFGSIMSLSDSDLWIAFAISSVSVLILLLFRRPI
                     LICLFDPDIATTLGIPSRLISYGIFTLIILVLVSSLQAVGAFLTLGLLVGPAATVYLL
                     TNKPSHLFWGGGIIGGLGSLLAFYLSFPLGWHLGATIILVLGVIFCTAYLYSSRCGRL
                     KQRGKNSSQVQ"
     gene            1812..2303
                     /locus_tag="PK077_04530"
     CDS             1812..2303
                     /locus_tag="PK077_04530"
                     /inference="COORDINATES: protein motif:HMM:NF014232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRN23597.1"
                     /translation="MFLTQIINGKSTKFCLCAKCAQERGLLDPDAFDLAEKLFPNLQG
                     QFGKEGAAAPSAPIPALQSLPLTSCPICSFTLQDYKNVGRLGCSECYNVFEEEILPLL
                     TQIQPDSHHHGKTPERAEKRETETHTISDLEQQLSLAVAREDYERAAKLRDQIKQLRT
                     PTN"
     gene            2309..3364
                     /locus_tag="PK077_04535"
     CDS             2309..3364
                     /locus_tag="PK077_04535"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: protein motif:HMM:NF002194.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HRN23598.1"
                     /translation="MLFDDLLNNPAKWMVESRDEHDIVLTSRIRLARNLTATPFPGWA
                     TRQQREETLKLTSGEARQIPVMKGGYYAELSGLTQQQKQLLVERHLISRELAARSEGC
                     AVLISRSQNASILFNEEDHLRLQYILPGIQLKKAWGAISKIDSELEARLPYAYNTRLG
                     YITACPTNLGTGMRASVMMHLPGLVISEQMQQVVQAAAQLNITVRGLYGEGTEATGNL
                     FQISNQSTLGDSEDQIVERMTRFTSDLAHQEWNARRRLLQSSSLQVKDRVSRAYGLLT
                     NATLLSTQEALALLSFLRMGASLDIFSHQALKNVNKTIMNIQPAHLARLSTTDQTTPE
                     HRDQIRADIIRKELSGN"
     gene            3393..>4655
                     /locus_tag="PK077_04540"
     CDS             3393..>4655
                     /locus_tag="PK077_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRN23599.1"
                     /translation="MNNFTPRAQQVLAHARREADRFNHHYIGTEHLLLGLLKLGKGVA
                     VTILENLGVELTAVRKQVEEQIGRGTEPQAEGNIPYTPRVRKVLAMANREAQELNHTY
                     VGTEHLLLGLIRDGDGVAGQILRHFGVDLEQARRELLDVLTPKYQMDADEDNIIPDDD
                     DDEEENESPAIPTDEPSSPSYSRQQKSKTPALQAFGRDMTQLARDGKLDPVIGRASEI
                     DRVIQILCRRNKNNPVLLGEAGVGKTAIVEGLAQEIAQGHVPELLRSKRVISLDLALM
                     VAGTKYRGQFEERLKAVMDEIRREGNVILFIDELHTIVGAGSAEGSMDASNIIKPALS
                     RGELQAIGATTLNEYRKHIEKDAALERRFQQVQVGEPSVEDTIQILAGIQPKYEEHHK
                     VHYTKEAVEAAARLSHRYLTGRFLPDKAI"
     assembly_gap    4656..4665
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4666..6126
                     /locus_tag="PK077_04545"
     CDS             <4666..6126
                     /locus_tag="PK077_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRN23600.1"
                     /translation="RQVAESLQINLHTVLRAYQQLRDEGLIELRRAINRYLTGRFLPD
                     KAIDILDEAGARKRVSQMTRPDHISNMEIRIAEIKERKDKAVETQLFEEAARLRDEEK
                     QAKAELQNMLETWRNSYETNYVPVTDEDVMSVLAKWTGIPLARMEEKETTKLLRMEEE
                     LKSKVIGQDEAASAIARALRRSRADIKDPRRPIGSFLFLGPTGVGKTYLARNLAEIMF
                     GTADALIQVDMSEYMEKHTTSRLIGSPPGYVGHDEGGQLTEAVRRRPYSVILFDEVEK
                     AHPDVMNLLLQILEEGSVTDSLGRKINFRNTIIILTSNVGAASAKRQSTMGFGAMAAD
                     NADYAAMKGKILEEARRQFRPEFLNRFDDISVFRMLERDDLERIVHLEADKLISRLKT
                     KNITLALSPEALNLIIKNGYDPQYGARPMRRAIERLLEDPLAESLLRGDVKPGDKIEA
                     VETDGTETLTFLHIQDTPPAKPKAPRKRTPRKPKVE"
     gene            6253..8799
                     /locus_tag="PK077_04550"
     CDS             6253..8799
                     /locus_tag="PK077_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-acetylglucosaminidase"
                     /protein_id="HRN23601.1"
                     /translation="MTSYFPKILLLFVLTSLFVFAEDIQKNREREAARSVIMRTVPSL
                     AKAPEKLQLEIIEKEDGHDVFETKASGGILNIRGSSGTALCRGFYDFLKTNRLGMVSW
                     DNKDIRWPAQLPDAAPRRIVSPFRNHYYFNVVTYGYTMPYWTWERWEKEIDWMALHGI
                     DMPLALVATEGIAVRVWKQLGLTEKEIEEFYTGPAHLPWQRMGNIVNHDGPLPASWHK
                     EQIALQHRILHRMKSLGMTPICPAFSGFVPRGILRLYPEAKLHRLGWGGWPQKNHAHF
                     LSPEEPLFLKIGRLYMQEWQKEFGKNTYFLADSFNEMELPANKGGIEARNSMLSSLGE
                     QIYRSISSDNPDAVWVMQGWMFGYQRNIWNADTLKALLSKVPDDKMLLLDLAADYNKT
                     FWRNGMNWDVFKGFFNKPWVYSVVPNMGGKCAMTGVMDFYANGHLEALNSSSRGRLSG
                     MGMAPEGIENNDVIYELVTDAAWRDRQEDVEQYLENYCRARYGNYPDSMKEAWNLFRR
                     TAYSNLKDHPRFNWQMKPGTRGCSVNTSEDFLKGLSLFVNTRGLEQSPLFRQDAVEMT
                     AHYLGIRMNEAILAALEALDEQEMENTKKCMEYFRKYALRADSLLEGHPTWRLSRWLS
                     FARSHGTSPEEKNKYEQNARRLVTRWGPPVDDYAAKIWSGLIRDYYLPRWEHFIQSRL
                     SEKNPDMGAWEEKWVRSTGVSAAHAPGDLVNACRQAIREAPPLPSSLKRKAAGSIIGN
                     WTSSTVSTEWSYLNWPVSSADLEKLRGVRFVFTSGSHALEIDGVELLENGKVIARDRH
                     AGLAGKTSHGNFYQLSLPKGIHANNGCSLRARVRSMGGKSSNGKLELVKE"
BASE COUNT         2039 a         2741 c         2368 g         1687 t
ORIGIN      
        1 ggcccttccc tgaacgacag ctactccttc tcatggaaat gaacagccca gcccctccct
       61 gcacccggga tcacatctgc tggggcgcgc acgccagcca cccggaccgc caccgcctgg
      121 aagtggacag cctcagcgtt tattatggaa gccttctcgc gctcaacggc atcagctttt
      181 ccatcacctg cggacacacg ctggctctga tgggtcccaa cggcgcagga aaatccacgc
      241 tgatcaaggc tctggccggg ctgatccgcc ccgattccgg aaaaatcctg tggaacggac
      301 gccccctgca cgacacgccg ggagaaatag cctacctgcc ccaacgttcc gacgttgact
      361 ggtccttccc catcaccgtc cgcgcgctgg tggaaatggg gcgttatcct tccctgggcc
      421 tgtggaaaaa attcgggagg catgaccggg atattgtgga aaagtccctt cacgctctcg
      481 gtatggaatc cctggcggac cgccagattt cagaactttc cggcggccag cagcaacggg
      541 cctttctggc gcgggctctg gcgcaggagg cccatgtcct cctgctggac gaacccttca
      601 cgggcctgga cgctcccgcc agccagtccc tgggaaggct gctggattcc ctggcggcgg
      661 aagggcgtct ggtcatcgcc tcccatcacg acttgaatac ggcggcggat atcttcgacg
      721 ccatcctgct gatgaaccgg gagctggccg ctttcggccc cccgaaggaa gtgctttccc
      781 ccgcacgcat ccgggagata tacggaatgg acccccaacc ggaaacccaa gcttcatgat
      841 ggatgatttt ctccaattcc tccgcgaacc catcgcgcag cggtccctgc tggcctgcgt
      901 catgatcggc ttcgccaacg gcttcgtcag cgggctggta atcctgaaaa aatccgccct
      961 ccaaatcggc acgctctcct gcgccctgct gccgggcatt gcggtggcgg tgctcctctt
     1021 cggccttacc cgctggagcc tgctgacggg ggctgtggtg gccgccctgc tggtgggtct
     1081 gggttccctc ttcgtctccc gtacatcccg gctgaaccag gacaccgccc tttccatcct
     1141 gcataccacg gctttcgctg cgggcttcat cgtgctggtc aggctgggcc tgcagcagaa
     1201 aattgacgac tggctgtttg gctccatcat gagcctgtcc gactccgacc tgtggatcgc
     1261 cttcgccatc agctccgtca gcgtcctgat cctgctgctc ttccgccgcc ccatcctgat
     1321 ctgcctcttt gacccggaca tcgccaccac gctgggaatc ccctcccgtc tcatcagcta
     1381 cggcattttc accctgatca ttctggtgct cgtttcctcc ctgcaggccg tgggagcgtt
     1441 cctgacgctg gggctgcttg tcggacccgc cgccacggtg tacctgctta ccaacaagcc
     1501 gtcccatctc ttctgggggg gaggaatcat cggagggctg ggctccctgc tggcttttta
     1561 cctgtccttc cccctgggct ggcatctggg cgccaccatc attctggttc tgggcgtcat
     1621 tttttgcaca gcctatttgt attcatcacg gtgcggacgt ttgaaacaac gcgggaagaa
     1681 ttcttctcaa gtacaataac aaagcccatt ttcatgacaa tccatgacac gcagactact
     1741 agtccgccac agggagcgcc attataatgc gggttgtgaa aaaatgtgag atatgctctg
     1801 caccggcaac ggtgttcctg acacagatca tcaacgggaa gtccaccaaa ttctgccttt
     1861 gcgccaaatg cgcccaggag cggggtctgc tggatccaga tgccttcgat ctggcagaga
     1921 aactgttccc gaaccttcag ggccaattcg gcaaggaagg cgcggcggcc ccctccgccc
     1981 ccattccggc cctgcaatcc ctgcccctca caagctgtcc aatctgctcc ttcacccttc
     2041 aggattacaa aaacgtagga cgcctgggct gcagtgaatg ctacaacgtc tttgaagaag
     2101 aaatcctccc gcttcttacc cagatccagc ctgattccca ccatcacggg aaaacgccgg
     2161 aacgggccga aaaacgcgaa acggaaaccc ataccatcag cgatctggaa caacagcttt
     2221 ccctggcagt ggcaagagag gattatgaac gcgccgccaa actccgcgat caaatcaaac
     2281 aattgcgcac gccgaccaat taacatacat gctctttgac gacttactca acaaccccgc
     2341 caaatggatg gtggaatcaa gggatgagca cgacatcgtc ctcacctccc gcatccgcct
     2401 ggcgcgcaac ctgaccgcca ccccctttcc cggctgggcc acacgccagc agagggagga
     2461 aacgctgaag ctcacttccg gagaagcgcg ccaaatcccc gtcatgaagg gaggctatta
     2521 tgcggagctc tccggactta cccagcagca gaaacagttg ctggtggaac gccacctcat
     2581 ttcccgggaa ctggcggcgc gttcggaagg ctgcgccgtc ctcatctcac gcagccagaa
     2641 cgccagcatc ctcttcaatg aggaggacca tctgcgcctg cagtatatcc tgcccggcat
     2701 ccagcttaaa aaagcatggg gggccatctc caaaatagac tccgaactgg aggccaggct
     2761 cccgtacgcc tacaatacgc ggctgggcta cattaccgcc tgtcccacca acctgggcac
     2821 gggcatgagg gcctccgtga tgatgcatct gccgggcctc gtcatctccg aacagatgca
     2881 gcaggtcgtt caggcggccg cgcagctcaa cattaccgtc cgcggccttt acggggaagg
     2941 gacggaagcc accggcaacc ttttccagat atccaaccag tccacgctgg gggacagtga
     3001 agaccagatt gtggaacgca tgacccgctt cacctccgac ctggcccacc aggaatggaa
     3061 cgcgcgcagg cggctgctcc agtcatcctc cctgcaggtg aaggaccgcg tctcccgcgc
     3121 ctacggcctt ctgaccaacg ccactctgct ttccacgcag gaggcgctgg ccctgctctc
     3181 cttcctccgg atgggagcgt ctctggacat cttttcccac caggcattaa aaaacgtcaa
     3241 taaaaccatc atgaacatcc agccggccca tctggcccgc ctgtccacca cggaccaaac
     3301 cactccggaa cacagggacc agatccgcgc tgatattatt cggaaagaac tttccggcaa
     3361 ctaatgcaac catacaaatt ttttgaccat ccatgaataa ttttacgcct agggcgcaac
     3421 aagtgctggc gcatgcgcgc cgggaagcgg accgcttcaa tcatcattac ataggaaccg
     3481 aacaccttct gctcggcctg ctcaaactgg gcaagggcgt ggccgtcacc atcctggaaa
     3541 atctgggcgt ggagcttacc gccgtgcgca aacaggtgga ggaacaaata ggccggggca
     3601 cggaacccca ggcggagggc aacatcccgt acacccccag ggtgcgcaaa gtcctggcaa
     3661 tggcaaacag ggaagcccag gaactcaacc acacgtatgt gggcacggaa cacctgctcc
     3721 tgggcctcat ccgtgacggc gacggcgtgg ccggacagat tctgcgccac ttcggcgtgg
     3781 atttggaaca ggcgcggcgc gaactgctgg acgtgctgac ccccaaatac cagatggatg
     3841 cggatgaaga caacatcatc ccggacgacg atgacgacga ggaggaaaat gaatcccccg
     3901 ccatcccgac ggacgagcct tcctccccct cctactccag gcagcagaaa tccaagaccc
     3961 ccgcgctcca ggcctttggc cgggacatga cccagctggc ccgggacggc aagctggatc
     4021 ccgtcatcgg ccgcgcctcg gaaattgatc gcgtcatcca gattctgtgc cggaggaaca
     4081 aaaacaaccc cgtcctgctc ggtgaagccg gggtgggcaa aaccgccatc gtggaagggc
     4141 tggcccagga aatagcccag ggccatgttc cggagctcct gcgcagcaag agggtcatct
     4201 ccctggacct ggcgctgatg gtggccggca ccaaataccg cggccagttt gaagaacggc
     4261 tcaaggccgt gatggatgaa atacgccggg aaggcaacgt catccttttc attgacgaac
     4321 tgcacaccat cgtgggcgcc ggctccgcgg aaggctccat ggacgcctcc aacatcatca
     4381 agcccgccct ctcccggggt gagctccagg ccataggcgc caccacgctc aacgaataca
     4441 ggaaacacat tgagaaggac gccgccctgg aacgccgctt ccagcaggtg caggtagggg
     4501 aaccctccgt ggaggacacc atccagattc ttgccgggat ccagcccaag tatgaggaac
     4561 atcacaaggt gcattatacg aaggaagccg ttgaagccgc agccaggctc tcccaccgct
     4621 atctgacggg ccggttcctg ccggacaagg ccatcnnnnn nnnnncgcca ggtggccgag
     4681 tcgctgcaga tcaacctgca cacggtgctg cgggcctatc agcagctgcg ggacgagggc
     4741 ctgatcgagt tgcgccgcgc cattaaccgc tatctgacgg gccgtttcct gccggacaag
     4801 gccatcgaca tcctggatga agcgggcgcg cgcaagcgcg tctcccagat gacccgcccg
     4861 gaccacatca gcaacatgga aatccgcatt gcggaaatca aggaacgcaa ggacaaggct
     4921 gtggaaaccc agctttttga agaagcagcc cgcctgcgcg acgaggaaaa acaagccaag
     4981 gcggaacttc agaacatgct ggaaacctgg cgcaactcct atgagaccaa ctacgttccc
     5041 gtgacggatg aagacgtgat gagcgtactc gccaaatgga cgggcatccc gctggcccgg
     5101 atggaggaaa aggaaaccac caagcttctg cggatggagg aagagttgaa aagcaaagtc
     5161 atcggccagg acgaggcggc ttccgccatc gcccgcgctc tgcggcgcag ccgtgcggat
     5221 atcaaggatc cgcgccgccc gatcggctca ttcctcttcc tgggccccac cggcgtgggc
     5281 aaaacctatc tggcccgcaa cctggcggaa atcatgttcg gcaccgcgga cgccctgatt
     5341 caggtggaca tgagcgaata catggaaaaa cacaccacgt cccgtctcat cggttctcct
     5401 cccggctacg taggccatga cgaaggcggc cagctcacgg aagccgtccg gcgcaggccc
     5461 tactccgtca tcctctttga tgaagtggaa aaggcccatc cggacgtcat gaacctgctt
     5521 ctccagattc tggaggaagg cagcgtgacg gattccctgg gccgcaagat caacttccgc
     5581 aacaccatca tcatcctgac ctccaatgtg ggggccgcct ccgccaagcg gcagagcacc
     5641 atgggctttg gcgccatggc cgccgacaac gcggactacg ccgccatgaa gggaaagatt
     5701 ctggaagaag cgcgcaggca attccgcccg gaattcctga accgctttga tgacatttcc
     5761 gtcttccgca tgctggaacg ggatgatctg gaacgtatcg tgcatctgga agcggacaag
     5821 ctcatctccc gtctcaagac caagaacatc acgctggccc tgagtccgga agccctgaac
     5881 ctgatcatca agaacggcta tgacccgcag tacggcgccc gcccgatgcg ccgcgccatc
     5941 gaacgcctgc tggaagaccc gctggcggaa tccctgctga ggggagacgt gaaacccggc
     6001 gataaaattg aagccgtgga aacggacggg acggagacgc tcaccttcct gcacatccag
     6061 gacacccccc cggcaaagcc caaagcgccg cgcaagagaa cgccgcggaa gcccaaggtg
     6121 gaataaaagc tttcagtctt ttcaaccagg gcccctgcat gacaacatgc aggggccttt
     6181 ttctttaacg gattacgcaa atatccgctt tttcctgaaa gaactattta cctgtcttgt
     6241 atatattaca atatgacctc ttatttccca aaaattctat tacttttcgt gctgacaagc
     6301 ctgtttgtct tcgcagaaga tatacagaaa aacagagagc gggaagcagc gcgttcagta
     6361 atcatgcgga ccgttccgtc cctggccaaa gccccggaaa aactgcaact ggaaattatt
     6421 gaaaaagaag acgggcacga tgtttttgaa acgaaagcct ccggcggaat actgaatatc
     6481 cggggaagca gcggcacagc cctctgccgg ggattctatg attttctgaa gacaaaccgt
     6541 ctgggaatgg tttcctggga taataaagat atccgctggc cggcgcaact gccggatgcc
     6601 gcgccgcgcc gcatcgtttc cccttttcgc aaccactatt acttcaatgt ggtcacctat
     6661 ggctatacga tgccttactg gacctgggaa cgctgggaaa aagaaataga ttggatggct
     6721 ctgcacggca tagatatgcc tctggccctg gttgccacgg agggaatcgc cgtccgggta
     6781 tggaaacagc tgggactgac ggagaaagaa atagaagaat tttatacggg accggcacac
     6841 cttccctggc agcggatggg gaacatcgtc aatcatgacg gccccctgcc cgccagctgg
     6901 cacaaagaac aaatcgccct gcagcaccgc atcctgcaca ggatgaaatc cctgggcatg
     6961 acccccattt gtcccgcttt ttccggtttc gttccccggg gaatcctccg cctttatccg
     7021 gaagcgaagc ttcaccggct ggggtgggga ggctggcctc aaaaaaacca cgcccatttt
     7081 ctttctccgg aagagcccct tttcctcaaa ataggacgcc tgtacatgca ggaatggcag
     7141 aaggaattcg ggaaaaacac ctattttctc gcagactcct tcaatgaaat ggaactccct
     7201 gcaaacaaag gaggcattga agcccgcaac agcatgctca gctcccttgg ggaacaaatc
     7261 taccgctcca tttcctctga caatccggac gcggtatggg tgatgcaggg atggatgttc
     7321 ggataccagc gcaatatctg gaacgcagat actctgaagg ctttgctcag caaggttccg
     7381 gacgataaga tgctccttct ggacctggcg gcggattaca acaagacttt ctggcgcaac
     7441 ggcatgaact gggacgtctt caagggtttt ttcaataaac cgtgggttta cagcgtggtt
     7501 cccaacatgg gcggcaaatg cgccatgacc ggggtaatgg atttttacgc caacggccat
     7561 ctggaagcct tgaactcttc atccaggggg cgtctctccg gaatgggcat ggccccggaa
     7621 gggatagaaa acaatgacgt tatctacgaa ctggttacgg acgccgcatg gagggaccgc
     7681 caggaggacg tggaacaata tctggaaaat tattgccgtg cccgatacgg aaattatccg
     7741 gacagcatga aggaagcctg gaatctgttc cgccgcacag cctattccaa cttaaaggac
     7801 catccccgtt tcaactggca aatgaaaccc ggcacccgcg gctgttccgt aaacacaagc
     7861 gaagattttc tgaaaggcct ctccctgttc gtcaacactc gcggcctgga acagtccccc
     7921 ctgttccgac aggatgccgt tgagatgacc gcccattacc ttggcatacg catgaatgaa
     7981 gccatcctgg ccgctctgga agccctggat gaacaagaaa tggagaatac gaaaaaatgc
     8041 atggaatatt tccgcaaata cgcccttcgg gcggattccc ttctggaagg acaccctacc
     8101 tggaggctgt cacgctggtt atcatttgcc cggtctcacg gaacttctcc ggaggaaaaa
     8161 aacaagtatg aacaaaacgc cagaaggctg gtgaccagat gggggcctcc cgtagacgac
     8221 tacgccgcca aaatatggag cggccttatc agggactact atctgccgcg ctgggaacac
     8281 tttattcaga gccgtctttc cgaaaaaaat ccggatatgg gagcatggga ggaaaaatgg
     8341 gtccgttcca ctggcgtaag cgcggcgcac gctcccggag atctggtcaa cgcctgccgc
     8401 caggctatcc gggaggcgcc gcccctgcct tcttccctga aaagaaaagc agccgggtca
     8461 attataggaa actggacatc ctcaacggta tccacggaat ggagttatct gaactggccc
     8521 gtttcttccg ctgatcttga aaaactccgg ggggtgcgct tcgtcttcac ttccggcagc
     8581 catgccctgg aaatagacgg cgtcgaactc ctggaaaacg gcaaagtaat cgcccgcgac
     8641 cggcacgccg gattggcagg caagacttcc catggaaatt tctatcagct ttcccttccc
     8701 aaggggattc atgccaacaa cggatgctcc ctcagagcca gggtccgctc catgggagga
     8761 aaatcttcca atgggaaatt ggaactggtt aaagaatgaa cagcttcaat ctcatttgct
     8821 tacggcttgt tcctcattga ttccg
//
LOCUS       DAOMSU010000071         8820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_562_length_8820_cov_3.393269, whole genome shotgun sequence.
ACCESSION   DAOMSU010000071 DAOMSU010000000
VERSION     DAOMSU010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8820
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_562_length_8820_cov_3.393269"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="PK077_04555"
     CDS             <1..264
                     /locus_tag="PK077_04555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HRN23602.1"
                     /translation="VDGLYPPGGRRKAIISRVEDVDAVLAFAEEDRGRFSMGGMSAKL
                     QAIREAVNAGTGVYMLHGRHPERIALLLEGKAEGAGTYFVPKG"
     gene            267..>1544
                     /locus_tag="PK077_04560"
     CDS             267..>1544
                     /locus_tag="PK077_04560"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HRN23603.1"
                     /translation="MENPLEPAGRTALRASREMLALEPAEKAQALRLMAAGVRSAAEK
                     IVRENGLDMEEARKGGRNAAFLDRLLLTPERVEHMARGMEQVAALPDPVGECIREWTR
                     PNGLHIRQVRVPLGVIGFIYESRGTVTCDAAALCLKSGNAVILRGGSESLRTNRALAE
                     VLRAALKESAVSADAVQLLDSGSRDEVRQLCGLDSFLNVIIPRGGKGLIRAVTEYARV
                     PVLKHLDGVCHVYVDAAADLEMAVNVLDDAKTQRPGVCNAAETLLVDAAAAERFLPMA
                     AERMRLRGVECRVCERSRPFFGPEAIPAEEEDWSTEYEDLILSVKVVDGVRDAVEHIN
                     HYGSHHSDSIITEDERARDYFMNRVDSACVYHNCSTRFSDGEEFGFGAEIGIGTDKFH
                     ARGPMALRELTSYKYVIEGDGQMKDPSRVPRKGR"
     assembly_gap    1545..1562
                     /estimated_length=18
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1639..7401)
                     /locus_tag="PK077_04565"
     CDS             complement(1639..7401)
                     /locus_tag="PK077_04565"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HRN23604.1"
                     /translation="MASASVSDGNLGNVMYIGDSITHGVNSGSWRWAMHKIFTDNGVS
                     YTEKGIMTGNFSGGVAAGTSYGGKIFQNIHSSAASARTYEIAGRANGSNYGRFDGSNI
                     KNWLGLSTEKTKGGSYAGQTFTGANKPDTFFLLSGTNDLLSDGDNNSLFYRLDGVTSL
                     LLGDMDSIVSSIRTANSQASILVMTIPCWTQHSNGNLAETHQAVASYNESLKEWGTNN
                     AANGVKVIDINAGIIDTASSTPFYGVRSMFNNPTADGLHPNAQGDLLMAGNLAKGMGY
                     AGRSAGQERKATHELEVNFGQNPSITQQTLEANGFTANNVTISNNTVNFNKSGSSSLT
                     YNWGAAQGQNKGYTVDFTLRLGNGATGGWDTTNNFSLNLGNGTLNINEAYIQWGSTIL
                     YSKDMSANADSIRVAYVAGDRANGLDAGYYIWLGDVLIGEAQTAAGGSSSGLTFSYSG
                     TLDAILSNLALDGSLSYAPSTTGITGPESLYLANGASPSTLKPGMIEWTAGTAATAVA
                     TAGTNATYNVRNTTNSNIASITGTGSTKFIYANNGAYGSAGNRQDLWVTLGAGTSSTN
                     GWIGGHTTGDLYGDIHLRLAEGAIGKSTVFGVVNAGTVHGDIYLEFSSSTGVYDTSFT
                     TGEADRTSVAGSYASAVNGDITMVFNDGVFSHRIFGGFYTGNKTISGSASLFINGGTF
                     MNEIYAGNKTDGTISRGTSLTVTGTDAILGKADGDNWTWTLLCAGNKTSGTINGGTTI
                     TLKDIAATTGAGTEHQFDKYAGIIDGKGAGTVNGEKKLIFDNYAANFLGTLQNFDKVQ
                     ITGNSDLSLDKSLGNTVTSLSVDAGSSLRFNRDQDATLDITNNGTIRTSHNLTLKSAD
                     TGTGTYQVEGGTLDLAGQAVSGKISLSAGALSNTAGLGAGSVSVSGITGNVNLGGLDG
                     SKLSSINMGSGTGIISGLTGHADLSGSDTQLRLSAANMGADGTGIIQFNDADNASLTL
                     GNLTLTLTADAVALLAAPGDYSFHVTNAALHADTNHITLSEYNGMAWDITRTEGGQVI
                     ISFGQLLDAMVIDKGASKNVTSANTLAATPSVTNNGTLNIDLSAATAPENKTTTIRYL
                     AGTEADAVVNTGTDSDVIITLLNETSADPDKPGNTAYAGSIEGAAQLVKDGEQRLTVD
                     GRVTASALDVQQGELALQNTKESSIISGALNVEQDAALTLNAASLAAGDLAGSGSITL
                     NGGALLSIARDTLSDLTLHASVTGSGTLKLDNCNLILGTDNNLGEDVLLHLGGGSLRL
                     QDGSAIALEGLHQEQENGALHLGADGRLELASNDGKTYSFKGDITGTGAITHRGEGTQ
                     IILSSGNAGVDVSHTRTGGHLVLGDKDLAANGLMTYRDIFIGSNTRDASDLDATADLS
                     LMNNTTSNSLSIASNGKLYLGGNPNQRAVQLVLTGNNGGVAATLASGASLDLTVNTET
                     LTNSADNAWAALKAENGSIRINGTDPSINVNIYGLGAAGDWAILPEFTVNAFYAENGL
                     VTADGETWTEDMVNLTYAGFANLVYDISKKLSDDGKLFQVHFTKQGDSPLKDFASTGN
                     QQGAADSLWAVTSSPENITPDMMEFLNAMGNAQAMGNAEAIRSALSSYAGSGITALHS
                     AQKGALRDQVLHLRNRLSQMGVSPQYIHDDLPGFNAWIEGEGGYRRLDDRTDESGYKL
                     STWGGTFGFDVTCSDSFVWGAAFSASYGDLDAYMANGDLDSYYGNLFFRIQSGRWAHN
                     IILTCGWNDASLDRTAGIPGAGMYTMHGSTSGSSYGAFYEGTYDLYLNENNTSALQPL
                     VNASVYRSRMDGFTESGGLGMNVDDMDSTFGTVGLGARLRGELSANLTGRASLGELRV
                     QVVQLLGDRDTAAVLAPAGIPGAGFRVNGAREGATGVQVGAGITIPVGYTGSVFADVN
                     ADFRSRANSFNGSIGYRLTF"
     gene            complement(7670..8497)
                     /gene="cysE"
                     /locus_tag="PK077_04570"
     CDS             complement(7670..8497)
                     /gene="cysE"
                     /locus_tag="PK077_04570"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HRN23605.1"
                     /translation="MTSHKRLKLRQCTNCECVINGVWEWVEECARGAAAEEPAMRGIL
                     DDVCLSRNSLATSISARLARKLSRRDMPRELIEPLIREALTEDQNILSQIAQDLIAIV
                     DRDPACRTPLEPLLFYKGFHAITTYRISHWLWQHDRTIMALQFQSLASEVFAVDIHPA
                     ATIGCGILLDHATSFVVGETAIIRDNVSILHEVTLGGTGKEEGDRHPIVESGVLIGAG
                     AKILGRVTIGACAKIAASSVVLENVPPHTTVAGVPAVIVNDSIPDNPALDMNQRLCN"
     gene            complement(8609..>8820)
                     /locus_tag="PK077_04575"
     CDS             complement(8609..>8820)
                     /locus_tag="PK077_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23606.1"
                     /translation="ALSVIILMAVAITKHVKNGYFSNWSGKKAGEGVEFHLLYAGSAF
                     ALFLTGPGPWSVDAWCMNIVHVIFG"
BASE COUNT         1619 a         2390 c         2842 g         1951 t
ORIGIN      
        1 gtggacggcc tgtatccccc aggaggaagg cggaaggcca tcatttcccg ggtggaggat
       61 gtggatgccg tgctggcgtt cgcggaggag gaccgggggc gcttttccat gggaggcatg
      121 agtgccaagc tccaggctat ccgggaggcc gtgaatgcgg ggacgggcgt ttacatgctg
      181 catggccgcc atccggaacg catcgcgctg ctgcttgagg ggaaggcgga aggagccgga
      241 acatattttg taccgaaagg atgaagatgg agaatcctct tgaaccggcg ggacggaccg
      301 cgctgagggc ctcacgggag atgcttgccc tggagccggc agagaaggcg caggccctgc
      361 gcctgatggc cgcaggcgtt cggagcgctg cggaaaagat tgtccgggag aacggccttg
      421 atatggaaga ggcccggaaa ggcggccgca atgccgcgtt tctggacagg ctgcttctga
      481 cgccggagcg tgtggagcac atggcccgcg gcatggaaca ggtggcggct ctccctgatc
      541 ccgtgggcga atgcatccgg gaatggacgc gccccaacgg gttgcatatc cgccaggtgc
      601 gcgttccctt gggggtcatc ggttttattt acgagagccg cggcaccgtc acctgcgatg
      661 ccgccgccct gtgtctgaag tccgggaatg ccgttattct gcgcggcgga agcgagtctc
      721 tgcggaccaa ccgggcgctg gcggaggttc tgcgcgccgc gctgaaggag tccgccgttt
      781 ccgcggacgc cgtgcagctt ctggattccg gcagccgtga tgaggtgcgg cagttgtgcg
      841 ggctggattc tttcctgaac gtgattattc ccaggggggg aaagggactg atccgcgccg
      901 tgacggagta cgccagggtt cccgtgctga agcatctgga cggcgtttgc catgtgtatg
      961 tggatgcggc ggcggattta gagatggccg tgaacgtgct ggatgacgcc aaaacgcaga
     1021 ggcccggcgt atgcaatgcg gcggaaaccc tgctggtgga cgctgccgcg gcagaacggt
     1081 tcctgccgat ggcggcggaa cgcatgcgcc tgcgcggcgt ggaatgccgc gtgtgtgagc
     1141 gaagccgccc gttttttggt ccggaagcca ttccggcgga agaagaggat tggtccacgg
     1201 aatatgaaga cctgattctt tccgtgaaag tggtggacgg cgtccgtgat gccgtggagc
     1261 acattaacca ttacggctcc caccacagtg attccatcat tacggaggat gaacgggcgc
     1321 gcgattattt tatgaaccgg gtggacagcg cgtgcgttta ccataattgt tccacgcggt
     1381 tcagcgacgg ggaggaattc ggcttcgggg cggaaatagg catcggtacg gacaaatttc
     1441 atgcccgggg gccaatggct ttgcgggaac ttacttccta caagtatgtt attgaaggag
     1501 acgggcagat gaaggacccg tcccgagttc cgcggaaagg ccgtnnnnnn nnnnnnnnnn
     1561 nngaggcccg ccgtaaacaa gaaagcaccg cggcatggcc gcgatgcttc cgttgatata
     1621 tactcctgaa gaagtcggtc agaaggtcag cctgtagccg atgcttccgt tgaagctgtt
     1681 ggcgcggctg cgaaagtccg cattgacgtc tgcaaagacg gagccggtat aacccaccgg
     1741 aatggtgatt ccggctccga cctgcacgcc ggtggctcct tccctggctc cgtttacgcg
     1801 gaaccctgcg ccgggtatgc cggcgggcgc caggacggct gcggtgtccc ggtcgcccag
     1861 aagctggaca acctgcaccc gcagttcacc cagggaggcg cggcccgtca ggttggcgga
     1921 cagttctccg cggagccttg cgcccagtcc cacggttccg aaggtggagt ccatgtcatc
     1981 cacgttcatg cccaggccgc cggattccgt aaagccgtcc atccggctcc tgtacacgga
     2041 ggcattcact agaggctgga gcgcggaagt gttgttctca ttcaggtaca ggtcatacgt
     2101 gccttcatag aaggcgccgt agctgctgcc gctggtgctg ccgtgcatgg tgtacatgcc
     2161 ggctccgggg attcccgcgg tgcggtccag ggaagcgtcg ttccatccgc aggtgaggat
     2221 gatgttgtgc gcccagcggc ccgactggat gcggaagaac agattgccgt agtaggagtc
     2281 caggtctccg ttcgccatgt aggcgtccag gtcgccgtag ctggcggaga aggccgcccc
     2341 ccatacgaag ctgtcgctgc acgttacgtc aaagccgaag gtgccgcccc aggtgctgag
     2401 cttgtagcct gattcgtccg tccggtcatc cagcctgcgg tagccgcctt ccccttcaat
     2461 ccaggcgttg aagccgggca ggtcatcgtg aatgtactgc ggggagactc ccatctggga
     2521 gaggcggttg cgcaggtgaa gcacctggtc ccggagggct cccttctggg cggaatgcag
     2581 ggcggtaatg ccgctgcccg cataggagga aagggctgac cggatagctt ccgcattccc
     2641 catggcctgg gcgttgccca tggcgttaag gaattccatc atgtccggag tgatgttttc
     2701 cggtgaggag gtgacggccc acagactgtc cgccgcgccc tgctggttgc ccgtggaggc
     2761 gaagtctttc agcgggctgt ctccctgctt ggtgaagtgg acctggaaca gcttgccgtc
     2821 gtcggagagt ttcttgctga tgtcatagac aaggttggcg aagccggcat aggtcaggtt
     2881 caccatgtcc tccgtccagg tttccccgtc cgccgtgacc agtccgtttt ccgcatagaa
     2941 ggcgttaacg gtgaattccg gcaggatggc ccagtctccg gcggcgccca ggccgtaaat
     3001 gttgacgtta atggatggat ccgttccgtt gatgcggatg gagccgtttt ccgccttcaa
     3061 ggcggcccag gcgttgtccg cgctattggt cagcgtttcc gtattgacgg tgagatccag
     3121 ggaagctccg gaggccagag tggccgccac gccgccgttg ttgccggtca acacgagctg
     3181 aacggccctc tggttgggat tgccgcccag gtacagtttg ccgttggagg cgatggaaag
     3241 tgagttggac gtggtattat tcatcaggga gaggtctgcc gtggcgtcca ggtcagaggc
     3301 gtcccgggtg ttgctgccga tgaaaatatc cctgtaggtc atgaggccgt ttgctgccag
     3361 atccttgtct cccagaacga gatgaccgcc ggtccgggta tggctgacgt ccacgcctgc
     3421 gttgccggag gaaaggatga tctgcgtgcc ttcgccccgg tgggtaatgg ctcccgtgcc
     3481 ggtgatatcc cctttgaagg agtaggtttt cccgtcgttg gaggccagtt ccagacgtcc
     3541 gtcagccccc aggtggagcg cgccgttctc ctgctcctgg tggagccctt ccagtgcgat
     3601 cgcggagccg tcctgcagac gcaggctgcc gccccccaga tggagcagca cgtcttcgcc
     3661 cagattattg tccgtaccca gaatcaggtt gcagttgtcc agtttcaggg tgccggagcc
     3721 ggtgacgctt gcgtggagcg tcaggtctga aagagtgtcc ctggcgatgg agagcagggc
     3781 gcccccgttc agggtgatgg agccggagcc cgccagatcc ccggcggcca gggaggcagc
     3841 gttcagcgtc agcgcggcat cctgttccac gttcagggcg ccggagatga tggaactttc
     3901 cttcgtgttc tggagcgcga gttctccctg ttgcacgtcc agagcggagg cggttaccct
     3961 gccgtcaacg gtcagcctct gttccccgtc cttgacgagc tgcgccgctc cttcaatgga
     4021 acccgcgtaa gcggtgttgc ccggtttgtc cggatctgcg gaggtttcat tcagaagcgt
     4081 gatgatcacg tcgctgtccg tccccgtatt cacgacggca tcggcttccg tgccggccag
     4141 gtagcggatg gtggttgttt tgttttccgg cgcggtggcc gcggacaggt caatgttcag
     4201 cgttccgttg ttggtgacgc tgggagttgc cgccagagtg ttggcggagg tgacgttttt
     4261 ggaagctccc ttgtcaatga ccatggcgtc cagcaactgg ccgaaggaga tgattacctg
     4321 gccgccttcc gtccgggtaa tgtcccacgc catgccgttg tattcgctca gcgtaatgtg
     4381 gttggtatcc gcgtgcaggg cggcgttggt gacgtggaaa gagtagtccc ccggagcggc
     4441 gaggagcgcc acggcatccg ccgtcagcgt cagggtcagg ttgcccagcg tcaagctggc
     4501 attgtccgcg tcattgaact ggatgattcc cgtgccgtcc gcgcccatgt tggcggcgga
     4561 aagcctcaat tgcgtgtcgg aaccggacag gtccgcatgg cccgtcagac cggaaatgat
     4621 tcctgtaccg ctgcccatgt taatggagct cagcttgctg ccgtccagtc cgcccaggtt
     4681 gacgttcccg gtgatgccgg agacggaaac gctcccggct cccagtcccg ccgtattaga
     4741 gagcgccccc gcggagaggg atattttccc ggagacggcc tgcccggcca ggtccagcgt
     4801 tcctccttcc acctgatagg tcccggttcc ggtgtctgcg gatttgaggg tcaggttgtg
     4861 cgaggtgcgg attgtgccgt tattagtgat gtccagcgtg gcgtcctggt cccggttgaa
     4921 ccggagggag gaacctgcgt ccacggacag ggaggtgacg gtatttccca gggatttgtc
     4981 cagcgagaga tccgaattcc cggtaatttg tactttgtca aaattctgga gcgttcccag
     5041 gaagttggcg gcgtagttgt caaagatgag cttcttctcc ccattcaccg tcccggctcc
     5101 cttaccgtcg atgatgccgg catatttgtc gaactgatgt tcggttccgg ctccggtagt
     5161 ggcggcgata tctttcaggg tgatcgtggt gcctccgttg atggtgccgc tggttttatt
     5221 gcctgcgcac agaagtgtcc atgtccagtt atccccgtca gccttgccca gaatggcgtc
     5281 cgtgcccgtg acggtcaggc tggttccccg ggaaatggtg ccgtccgtct tgttcccggc
     5341 gtagatttca ttcatgaacg ttccgccatt gatgaaaagg gaggcggagc cggagatggt
     5401 tttatttccc gtgtaaaagc cgccgaaaat gcggtgggag aatacgccgt cattgaacac
     5461 catggtgatg tccccgttca cggcggaggc ataggagccc gccacggagg tgcggtccgc
     5521 ttctccggtg gtgaaggaag tatcgtaaac cccggtggat gaggagaatt ccaggtaaat
     5581 gtccccgtga acggttccgg cgttcacaac gccgaatacc gtggatttgc cgatggcccc
     5641 ctccgccagg cgcaggtgga tgtcaccgta caggtctccc gttgtatggc cgcctatcca
     5701 gccgttggtg ctgctggttc ccgctcccag cgtcacccac aggtcctgac ggttacccgc
     5761 gctgccgtaa gcgccgttat tggcgtaaat gaatttggtg cttcccgtgc ccgtaatgct
     5821 ggcaatgttg gaattggttg tgttacggac attatacgtg gcattggttc cggcagtggc
     5881 tacggcggtg gcggcggttc ccgcagtcca ttcaatcatg cccggtttga gagtggaggg
     5941 agacgcgcca tttgccagat aaagagattc agggccagta atgccggtgg tagagggggc
     6001 gtaggaaagg gaaccgtcca aagccagatt ggacaggatg gcgtccagcg tgccggaata
     6061 gctgaacgtg aggccggaag aacttccccc cgctgccgtc tgcgcttccc cgatgagaac
     6121 atcccccagc cagatgtaat agcccgcgtc caggccgttg gcgcggtctc ccgccacgta
     6181 ggctacgcgg atggagtccg cattggcgga catgtctttg gaataaagaa tggtgctgcc
     6241 ccattggata tacgcttcat taatgttcag cgttccgttt cccaggttca gggagaaatt
     6301 gtttgtggtg tcccagccgc ccgtggcccc gttgcccagg cgcagagtga aatctaccgt
     6361 gtagcccttg ttctgtccct gggcggctcc ccagttatag gtcagggagc tgctgccgga
     6421 tttgttgaag ttgaccgtat tattggagat ggtaacgtta ttagcggtga agccgttggc
     6481 ctccagcgtc tgctgggtga tggacggatt ttgtccgaag ttaacctcca gctcatgggt
     6541 ggctttcctt tcttgcccgg ctgaccgccc ggcatagccc atgcctttgg ccaggttgcc
     6601 tgccatcagg agatcgcctt gggcattcgg gtgcaggccg tccgccgtag ggttgttgaa
     6661 catggagcga accccgtaga acggcgtgga ggaggcggta tcgatgatgc ccgcgttgat
     6721 gtcgatgacc ttgacgccat tggcggcgtt attcgtcccc cattccttca gggattcgtt
     6781 gtaggaggct acggcctggt gcgtttcagc caggttcccg ttggaatgtt gcgtccagca
     6841 gggaatggtc atcactaaaa tgcttgcttg gctgttggcg gtacggatgg aagagacgat
     6901 ggaatccatg tctcccagca aaagggaggt aactccatcc agacgataga acagtgaatt
     6961 attatcgccg tcggaaagga gatcgttggt cccggagagc aggaaaaacg tgtccggttt
     7021 attggctccg gtaaaagtct gtcctgcata ggaccctccc ttggtctttt ccgtgctcag
     7081 gcccagccag tttttgatgt tggatccgtc aaatcgtccg tagttgctgc cgttcgctct
     7141 cccggcgatt tcataagttc tcgcggaagc cgcagaggaa tggatattct ggaaaatctt
     7201 accaccgtaa gaggttccgg cggccacgcc tccgctgaaa ttaccggtca taatgccttt
     7261 ttcagtatag ctgacgccgt tatccgtgaa aattttatgc atggcccagc gccacgagcc
     7321 gctgttgacg ccatgggtga tggagtcccc tatgtacatg acgttgccga ggtttccgtc
     7381 tgaaacggag gcggatgcca cggttgtaaa caggacactg cacgcaagca aagcagacct
     7441 tagagagagg ggtaatctta aacgcatatc ccatatagta tgcggattgg gaattaatta
     7501 tcaactcaaa atgttcaaaa taagcgcatt gagaatttca atgcattgag gttgaaacta
     7561 ttatattatg tccggtcttt tcagaattta cctttcccgc tgatgtttgc ggagttgcgt
     7621 atttaattcg ttttctgctg taagtgacgg atttctaatc tgttatctat tagttgcaaa
     7681 ggcgctggtt catgtccagt gcgggattgt ccggaatgga atcgttgacg ataacggcgg
     7741 gtacgcccgc taccgtggta tgggggggga cattttccag aacgacggag ctggctgcga
     7801 ttttggcgca ggcgccaatg gtgacgcgcc ccagtatttt agcccccgcg ccgataagaa
     7861 ctccggattc cacgatggga tggcggtccc cttcttcctt gcccgtgccg cccagcgtga
     7921 cttcatgcag gatggagacg ttgtcccgga tgatggcggt ttcccctacc acgaaactgg
     7981 tggcgtggtc cagcaggatg ccgcagccga tggtggccgc cggatggatg tccaccgcaa
     8041 atacttcact ggccaggctc tggaactgaa gggccatgat tgtccggtca tgctgccaca
     8101 gccagtggga gatgcggtac gtggtgatgg cgtggaatcc cttgtaaaaa agcaggggtt
     8161 ccagcggcgt acggcaggcg ggatcccggt ccacgatggc aatcaggtcc tgggcgattt
     8221 ggctgaggat gttctggtct tccgtcaatg cttcccggat aaggggctca atcagctccc
     8281 gcggcatgtc acggcgggag agcttgcggg ccagccgtgc cgagatggaa gtagccaggg
     8341 aattgcggga caggcagacg tcatccagaa taccgcgcat ggcgggttct tccgccgctg
     8401 cgccgcgggc gcattcctcc acccattccc atactccgtt aatgacgcat tcgcagttgg
     8461 tgcactgccg gagttttaag cgtttatggc tggtcatggc ggaaagggtt aacgaggcaa
     8521 aagaaagcct ttccataaat accagaatac ggcgggtttg agaatcggaa agtatgttct
     8581 atgcccttcc gtgtgctgtc tgccgccgtc aaccgaagat gacatgcacg atgttcatgc
     8641 accaggcgtc cacagaccaa gggccggggc ctgtcaggaa cagggcgaaa gcggagcccg
     8701 cataaagcag gtggaattct acgccttccc cggctttttt gccggaccag ttggaaaagt
     8761 agccgttttt gacgtgtttg gttatggcta cggccatgag gatgataacg ctgagagccg
//
LOCUS       DAOMSU010000072         8817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_563_length_8817_cov_2.538576, whole genome shotgun sequence.
ACCESSION   DAOMSU010000072 DAOMSU010000000
VERSION     DAOMSU010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8817
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_563_length_8817_cov_2.538576"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="PK077_04580"
     CDS             complement(<1..962)
                     /locus_tag="PK077_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23607.1"
                     /translation="MTIPLPPALPPHFNIGGYKITALVESGPDYHLYQALSPEGHAVL
                     IREFCPGGLVTRDLASGELAVFPENESQFAQAREAFETQYAANAESKLRGFGTVLFLY
                     PLSPAQPQPAVAHAQALRPAKKPQQPQLRKPVVGATIPGTPLPRVKHSGGFPVITVIV
                     TGMLALFGFLGYQILKDKEEPVAKAVTVPVPAPPKPKSKPAPPKPEPVVVAPEPEPEP
                     EPEPPAPDLSPSPEVIAMEKALREEAIASKGKFSEKLLNKYPYYAEAYVRDYVKKRGG
                     SFSPDFEKWLKNTKNNREVFAMFFPPDPSVATNVAFMIDELGLET"
     gene            complement(1156..1551)
                     /locus_tag="PK077_04585"
     CDS             complement(1156..1551)
                     /locus_tag="PK077_04585"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRN23608.1"
                     /translation="MTSSSRKSSTTWEPRSWAFPPCLPARKVTALPYKIIQKDANYKS
                     GTYGVLRSKSTGAVVNGDYNARAGGAPAGTYFDPAPMPYWMRITGGYGMHVGFVTGYP
                     VSHGCVRLPEDMAKTFFEHTPIGTKVTIR"
     gene            1928..2497
                     /gene="pyrE"
                     /locus_tag="PK077_04590"
     CDS             1928..2497
                     /gene="pyrE"
                     /locus_tag="PK077_04590"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HRN23609.1"
                     /translation="MSEQYDLLKSILLEKSVRTGTFTLASGKVSDLYVDCRMTALDPV
                     GATLVGALGWKLVKEDILPRFPEVAAIGGMTMGADPISLAVGMTSALEGAEKKLQVFT
                     VRKEPKDHGRGRRIEGNFQAGDTVVVVDDVITTGGSTLKAIDAIEAEGGRVAAALVLL
                     DREEGGRQAIEERGIPVYPLYTRTSLLGR"
     gene            2511..3572
                     /gene="nagZ"
                     /locus_tag="PK077_04595"
     CDS             2511..3572
                     /gene="nagZ"
                     /locus_tag="PK077_04595"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HRN23610.1"
                     /translation="MLPALIGISGHEVGAEEEAAIRRWQPAGFILFSRNIDSVEQVRG
                     LTESLRKLCLHHPVIAVDQEGGRVVRTASLGLNLPSPASLARLGSVGGVVELGAVTAL
                     ALRYLGVNLNFAPVLDICHDPSAANALPGRCWGDNAQDVISRGGVYASNLRRGGVQSC
                     GKHFPGMGRALADPHFSLPVVDLDERELFKTDLLPFLALCPALSSIMSAHIMLPQIDP
                     DYPATLSERVIRGLLRDRLGFRGVVFTDDLCMGAITAQYSPDDAAFLSLRAGCDLPLI
                     CHDPFPWLDGLASRLEGLNAYDREDSFKRVEKLSDSLCFPFPEKASLWDSCLRRAEAL
                     CRLEEDGGEKLPSSPVQKY"
     assembly_gap    3596..3605
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3665..4561)
                     /locus_tag="PK077_04600"
     CDS             complement(3665..4561)
                     /locus_tag="PK077_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HRN23611.1"
                     /translation="MAATPWASDRNWELVFEDDFDGSSLNEHNWSRIDYVGYNAPDWR
                     KYQSRDESLVEFREKDGNSAMTLWGKYGDYTTQTNQTAPAKTYACGGVYSLKTFSFQY
                     GYVEVRARFDCVQGVWPAIWMMPKSDSIGWPVGGEIDIMEHLNYEGRVYQTIHWSQNG
                     VPNQDKSQGVTPGWNDGAEKANWHTYGMEWTEEGITFYVDGKATGSFKKPNNANWPFD
                     KDGNEFYLIIDQQIGGNWVENAGVNKGIDQNTLANSGAAFDIDYVKVYSSSIYNHLVP
                     EPAAASLGLFGAALLAARRKRS"
     gene            4655..>4847
                     /locus_tag="PK077_04605"
     CDS             4655..>4847
                     /locus_tag="PK077_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23612.1"
                     /translation="MSIDKKLAGGLGMEKEIPSVPKRDCSVFSAGALRLALLPGFSYH
                     KGFLSFPAAGGRSLRAESHI"
     assembly_gap    4848..4857
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4858..>7982)
                     /locus_tag="PK077_04610"
     CDS             complement(<4858..>7982)
                     /locus_tag="PK077_04610"
                     /inference="COORDINATES: protein motif:HMM:NF039401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6288 domain-containing protein"
                     /protein_id="HRN23613.1"
                     /translation="YTVTQVIEGSPAEKYVKEGDLILGIDGHLFKTSQSLDVLYGPYQ
                     HQNRRGLDMHAGLLVDKAEGAGKITLNLIPAESVEKIQGIQPLWKEVFREEQAKKPVS
                     LSIPVKGGQQVRLRVDDGGNGIGSDGFEWSDLRLEGPGGTVPLTKAQQYTVGYGEARY
                     DAKSKIWQAHAVSSLVFDIPKGDWNLKGTGTPRWSASVGVTVQVGGSSALPDAVKKYV
                     KNVTFKIPQLGSYALGFPKNCAKSKAVVHMMSEWLAAQQREDGSWERPGGYCGNHYDT
                     GWAGLALMATGNPKYDPAIKKAAQYIAFSGSQCWWAVPQASAGIFLCEYWLRYRDNSV
                     LPAIRNSVQRMKNEVLYGDFVTGHGIHPGYRGTGVSIGGSHMCLFLALASKTPARTED
                     GVLDKMMDHAQSICPTGMGPYGRMTETFTFEPDRECGGTYSGRHGPYYIASLICGGPE
                     LYTKNSRIMYGEGPIGGCDQGHSSETLSIMWALPAYWRTNPEAYYKNMEAFRWKLTLL
                     RPFDGGMMQNPNRLELMTADSVIGTYIRTSIWITALCAERQNLAITGKPEFQAKTFRK
                     VPPIIDTESRFLNTYVRNWSMVNAALGTKAPASLKSAIRELKNIPVEQGCRFKLINVV
                     NNRALPIAKAIMEIPGVDQLTRATCAEMILGMDVRIFFEPKLNDGNPQPGEYSLNVDV
                     QQPLGGRALGLHRDKELEGKANSAYKYDFAGTVQFSDTTTFSPMETISWTPNSKFGGQ
                     WNVYSYKKELSGPTQPGVQKMNAKIKWRVNDLEVEYDRPIAVGGFEVGCAEKALSVTN
                     CNHLWVPGILIRDHGNWGCSFHLPDGTYISAASQGNQIEVYDKTDKKKEKTWVSPNDS
                     CLTQGSRCLFRVSTDWHGLECRVRELKLLGSSTEEVEDYKLKASTGGRVDTAKLMDRD
                     ATTVEELDASPTEDEPLVLELTLKSPAALRAVDIKIEKGHNRLIIEAYKGGKWIPIHW
                     GSLGASTAGVSDAQKAMYANEPEVLRMLQLPGNGFIKCMRTFDPVTTNRLRVKLFQKG
                     GKVRLAELHVY"
     assembly_gap    7983..8009
                     /estimated_length=27
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8010..>8817)
                     /locus_tag="PK077_04615"
     CDS             complement(<8010..>8817)
                     /locus_tag="PK077_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23614.1"
                     /translation="RDQRTLTTPGTGATPVHAGSLPQLRKKKNSAGIWILTLLLLGGA
                     CGTYYFTRNPAEKPVSAQEPDVPHSNREKKAAPPAVRGEASPEQENAAGLSPSGLAEQ
                     PEKTPPATSSGERNEDAPGALEEKSAATHAQPDENSSAPADDAADGKQPEPQQPANRE
                     ESQEGQTAAKPAPVVRPRGKAARQTTNMGDVNAYVKKFAKPMPLNQWRKQYAELYTSW
                     FGNSEEVAKGWITTFGPLGFRARGLDASWPDTNFRGFFPKTMLDPNGEPVA"
BASE COUNT         1568 a         2373 c         2760 g         2069 t
ORIGIN      
        1 gtttccagcc ccagttcatc aatcatgaaa gccacattgg tggcgacgct ggggtccggc
       61 gggaaaaaca tggcgaagac ctcacgattg tttttcgtgt ttttcagcca tttttcaaaa
      121 tccggagaaa aactgcctcc gcgttttttt acgtaatccc gtacgtaggc ttccgcgtaa
      181 tagggatatt tgttcagcag tttttcagaa aatttcccct tggacgcgat agcttcctcc
      241 cgtaaagcct tttccatggc aatgacttcc ggggaggggg aaaggtcggg agcaggcggt
      301 tccggttcag gctccggttc cggttcggga gcgaccacca cgggttccgg ttttggcgga
      361 gcgggcttgg atttgggctt gggcggagcc ggtacgggca cggtaacggc tttagcgacg
      421 ggttcttctt tatccttgag tatctggtag cccagaaatc cgaagagggc gagcatcccg
      481 gtcacaatga ccgtgatgac cgggaaccct ccggagtgct ttacccgcgg cagcggcgtt
      541 ccggggatgg tggcgccgac tacgggtttc cgcagttgcg gttgctgcgg tttttttgcg
      601 gggcggagag cctgggcatg cgctacagcc ggctggggct gcgccggaga aagcgggtaa
      661 aggaagagca cggtgccgaa tcccctcagc ttgctttctg catttgctgc gtattgggtt
      721 tcaaaagctt cccgggcctg ggcgaattgg gattcatttt ccggaaagac agccagttct
      781 ccgctggcca ggtcacgcgt aacgaggccc ccggggcaga attcgcgtat caggacggca
      841 tggccttccg gggaaagagc ctggtacaga tgatagtccg gaccgctttc tacaagcgcc
      901 gtgattttat agccgcctat gttgaaatgg ggcggcaggg cagggggaag agggattgtc
      961 atgactaatc aagaggtcaa agatcccgta tattcaaaaa ggttcggagc cgtttgtcaa
     1021 tgccggagtg aggaagctgc gccatgaaac gggagcagtt atcagcacgg aacgcttccg
     1081 gacggcggga atacgaaaca cgccggtttc cggaaagtgt ggcatttctg cggcgcgatg
     1141 ccgcgcagga aacggctaac ggatggtgac cttggtgccg atgggagtgt gttcaaagaa
     1201 tgtcttggcc atgtcctccg gcaggcgcac gcagccgtgg ctgaccggat atccggtgac
     1261 aaaacccaca tgcatgccgt agcctcccgt aatccgcatc cagtagggca tgggggcggg
     1321 gtcgaagtag gttcctgccg gcgctccgcc agccctggcg ttgtaatccc cgttgacgac
     1381 ggctccggtg gatttgctgc gcagaacgcc gtatgtgccg gatttgtagt tggcgtcttt
     1441 ctggatgatt ttgtatggga gtgccgtaac cttccttgcc ggaagacatg ggggaaacgc
     1501 ccacgagcgt ggttcccacg tagtagaaga ctttctgctg cttgaggtca atgatgatgt
     1561 gcttttcccc ctgggtgccc gccggcaggt cccaatatcc ctggcccaga cgggcttccg
     1621 gaggcaggtc cgtgttgacg cctgaggtgg cgcccactcc ggacagataa tcgtcccgtc
     1681 cggacattcc ctgaattttt ccggtgattt ggtcaaacag cgggggcctc tcgtacgatg
     1741 tgcaggaaac aagagcggaa ctgatagaaa ctaacgccag tatgccggat ttcatgggaa
     1801 cgggaaaatg acaggcaggt tatggggcct gtcctccttt ttgtcaagat ttgcatgctg
     1861 ttatccgttg cttattccgg gggtatctgt tatatacggc gaaccatttc attcatccct
     1921 atttttcatg agcgaacaat acgatcttct taaaagcatt cttttggaaa aatccgtgcg
     1981 cacgggaact tttacactgg cttccggcaa ggtgtccgac ttgtatgtgg attgccgcat
     2041 gacagccctg gaccccgtag gagccaccct ggtgggcgct ctgggctgga agctggtgaa
     2101 agaggatatt ttgccccgtt tcccggaggt ggcggccatc ggcggcatga ccatgggggc
     2161 ggaccctatt tccctggccg tcggcatgac cagcgctctg gaaggagcgg aaaagaagct
     2221 tcaggtgttc accgtgcgca aggagccgaa ggaccatgga cgcggccgcc gcattgaagg
     2281 caatttccag gccggggata ccgtggttgt ggtggatgac gtcatcacga caggaggctc
     2341 cacgctgaag gctattgacg ccattgaggc ggaagggggc agggttgccg ccgccctggt
     2401 gctgctggac cgcgaggaag gcggccgcca ggcgattgag gaacgcggca ttcccgttta
     2461 cccgctgtac accaggactt ccctgttggg ccgttaatcc ttttccgttc atgctcccgg
     2521 ccctgatcgg cataagcggc catgaagtgg gcgcggagga agaggctgcc atccggcgtt
     2581 ggcagccggc gggtttcatt ctgttttcca ggaatattga ttccgtggag caggtgcgcg
     2641 ggctgacgga gtcgctgcgg aaactttgcc tccaccatcc tgtcattgcc gtggaccagg
     2701 aggggggccg tgtggtgcgc actgcttccc tgggcctgaa tttgccctcc ccggcttcgc
     2761 tggcccggct cggttcggtt ggcggcgtag tggaactggg cgcggtgacg gctctggctc
     2821 tccgctacct gggggtgaac ctgaattttg ccccggtgct ggatatctgc catgatccgt
     2881 ccgcggccaa cgcgcttccc ggccgctgct ggggagataa tgcccaggac gtcatttcac
     2941 gcggcggcgt ttatgcctcc aacctgcgcc ggggcggcgt gcagagctgc ggcaaacatt
     3001 ttcccggcat gggccgtgcc ctggcggatc cccatttcag ccttcccgtg gttgacctgg
     3061 atgaacggga gctgttcaag acggatttgc tgccttttct ggctctgtgc ccggcattgt
     3121 cctccatcat gtccgcccat atcatgctgc ctcagattga tcccgattat cccgccacct
     3181 tgtccgaacg ggtgatcagg gggcttctgc gcgaccgcct cggtttccgg ggcgtggtgt
     3241 ttacggatga tttgtgcatg ggggcgatta cggcgcagta ttcaccggat gacgccgctt
     3301 tcctgtccct gcgggccgga tgcgatcttc ccctgatttg ccatgaccct tttccctggc
     3361 tggatgggct ggcttcacgt ctggaaggtt tgaacgccta tgaccgggag gattctttca
     3421 aacgggtgga aaagctgagc gactccctgt gtttcccgtt tccggaaaag gcttccctgt
     3481 gggattcctg cctccgccgt gcggaggctc tgtgccgcct ggaagaggac ggaggagaaa
     3541 agctgccttc atccccagtc cagaaatatt gaggcaggtg ccaatgacgg tttttnnnnn
     3601 nnnnncggga atgtcccgga agatttattt ccgcggcagg gggaataaaa agagtgctgg
     3661 ctgatcagct tcttttgcgg cgagccgcca gcaaggctgc tccaaacagg cccagggaag
     3721 ccgcggcggg ttccggaacg aggtggttgt agatgcttga ggaatagact ttgacataat
     3781 cgatgtcgaa tgcggctccg gaattggcca gcgtattctg gtcgattccc ttattaactc
     3841 ctgcattttc cacccagttg cctccaatct gctggtcgat gatcaggtag aattcgtttc
     3901 cgtccttgtc aaagggccag tttgcgttat tgggcttttt gaatgaaccg gttgcttttc
     3961 catccacata aaaggtgatg ccttcttccg tccattccat cccgtaggta tgccagtttg
     4021 ctttttcagc accatcgttc caaccggggg tgaccccctg ggatttatcc tgattgggaa
     4081 cgccgttttg cgaccagtgg attgtctggt aaacacggcc ttcgtaattc aggtgttcca
     4141 tgatgtcaat ttcccctccg acaggccagc cgatgctgtc ggatttgggc atcatccaga
     4201 tggccggcca gacgccctgc acgcaatcga atctggcgcg gacttctacg tagccatatt
     4261 ggaaggagaa ggttttcagg gaatataccc ctccgcaggc gtatgttttg gccggtgcgg
     4321 tctggttggt ttgagtagtg tagtccccgt attttcccca caaggtcatg gcggagttgc
     4381 cgtccttttc ccggaattcc acaaggcttt cgtcccggga ttggtacttg cgccagtccg
     4441 gggcgttata gcctacgtaa tcaatgcggc tccagttatg ttcgttcaga gatgagccgt
     4501 caaagtcgtc ttcaaaaacg agttcccagt tcctgtcgga tgcccaggga gtcgccgcca
     4561 tgacggaaga tgctgaaagt gagagaaaga gaagaggaaa aaggagtgcc gttctgatga
     4621 acatcatgag aaaatataga gcgggcgccg ctgagtgtca atagacaaaa aattggcagg
     4681 cggcctcggc atggagaagg aaattccctc cgtccctaaa agggattgct ctgttttctc
     4741 ggcaggggcg ctgcgattgg cgctcctgcc gggattctct tatcacaaag gctttttgtc
     4801 atttccagct gcgggcggcc gaagcttaag agcggagagc catatttnnn nnnnnnntgt
     4861 acacgtgcag ttccgcaagg cggacttttc cgcccttctg gaagagtttg acgcgcagcc
     4921 tgttggtggt gacggggtca aatgttcgca tgcatttgat gaatccgttg cccggaagct
     4981 ggagcatgcg cagcacttcc ggttcattgg cgtacatggc tttctgcgca tcgctgacgc
     5041 cggcggtgga ggctcccagg gaaccccagt ggatggggat ccacttgccg cccttgtagg
     5101 cctcaatgat gaggcggttg tggccctttt caatcttgat gtccacggcg cgcagggcgg
     5161 caggggattt cagggtgagt tccagcacca ggggctcgtc ttccgtgggg gaagcgtcca
     5221 gttcctcaac ggtagtggcg tcccggtcca tcagtttggc cgtgtccaca cggcctccgg
     5281 tggaggcttt cagcttgtag tcctccactt cctccgtgga acttcccagc agtttcagtt
     5341 cacgcacgcg gcattccagg ccgtgccagt ccgtggaaac gcggaacagg cagcgggaac
     5401 cctgagtcag gcaggagtca ttcggggaaa cccaggtttt ttccttcttt ttatccgttt
     5461 tgtcgtacac ctctatctgg ttgccctgag aggccgcgga aatgtaggtt ccgtccggca
     5521 gatggaagga gcagccccag ttgccgtgat cccggatcag gatgcccggc acccacagat
     5581 ggttgcagtt ggtgactgag agggcctttt ctgcacatcc cacttcaaag ccgcccacgg
     5641 caatggggcg gtcgtattcc acctccaggt cattgacgcg ccatttgatt ttggcgttca
     5701 ttttttgcac gcccggctgg gttgggccgc tgagttcctt cttgtaggag tagacgttcc
     5761 attggccgcc gaatttggaa ttgggcgtcc aggaaatggt ttccatgggg gagaaggtgg
     5821 tcgtgtcgct gaattgcacc gtgccggcaa aatcatattt gtaggcggag ttggctttcc
     5881 cttccagctc cttgtcgcgg tgaagcccca gggcgcggcc gcccagaggc tgctggacgt
     5941 ccacgttcag agaatattcc ccgggctggg ggttaccgtc gttgagcttg ggttcaaaga
     6001 aaatgcgcac atccatgccc agaatcattt ccgcgcaggt agccctggtg agctggtcca
     6061 ccccgggaat ttccatgatg gccttggcga tggggagggc tcggttgttg acgacattga
     6121 taagcttaaa gcggcagccc tgttccaccg gaatattttt caattcccgg atggcggatt
     6181 tcagggaggc gggggccttg gttccaagcg cagcgtttac catggaccag ttgcgcacgt
     6241 aggtgttcag gaagcgggat tccgtgtcaa tgatgggggg caccttccgg aacgttttgg
     6301 cctggaattc aggcttgccc gtaattgcca gattctggcg ttccgcgcag agggccgtga
     6361 tccagatgct ggtgcggatg taggtgccga tgacggagtc cgccgtcatt agttccaggc
     6421 ggttggggtt ctgcatcatg ccgccgtcaa aggggcgcag cagcgtcagt ttccaccgga
     6481 aagcctccat gtttttgtaa tatgcttccg gattggttcg ccagtaggcg gggagagccc
     6541 acatgatgga gagcgtctcg gaggagtggc cctggtcaca gccgccgatg gggccttccc
     6601 catacatgat gcggctgttt tttgtgtaca gctccggtcc gccgcagatg agggaggcga
     6661 tgtagtacgg gccgtgacgg ccggagtagg ttcccccgca ttcacggtcg ggttcaaagg
     6721 tgaaggtttc cgtcatgcgg ccatagggcc ccatgccggt ggggcagatg gactgggcat
     6781 ggtccatcat tttgtccagc acgccgtctt ccgtgcgcgc cggagttttg ctggccagcg
     6841 ccaggaacag gcacatgtgg gaacctccga tgctgacacc tgttccccgg tagcccgggt
     6901 gaatgccgtg gccggtgacg aagtccccat aaagaacttc gtttttcatg cgctgcacgc
     6961 tgttgcggat ggccggaagc acggagttgt cccggtagcg cagccagtat tcgcacagga
     7021 agatgcccgc ggaagcctgg gggacggccc accagcattg ggagccggag aaggcgatgt
     7081 actgagccgc ttttttaatg gcggggtcat acttcgggtt gcccgtggcc atcagggcca
     7141 ggccggccca gcctgtatca taatggttgc cgcagtagcc gcccgggcgt tcccaggagc
     7201 cgtcctcgcg ctgctgggcc gccagccatt cgctcatcat gtggactacc gctttgctct
     7261 tggcgcaatt tttggggaag ccgagggcat aggaaccaag ctgggggatt ttgaaggtga
     7321 cgtttttcac atatttcttc actgcgtccg gcagggcgga ggagccgccc acctgtacgg
     7381 tcacgcccac ggaggcggac cacctggggg ttcccgttcc tttaaggttc cagtcaccct
     7441 tggggatatc aaagaccagg gaagagacgg cgtgcgcctg ccatattttg cttttggcgt
     7501 cgtacctggc ttctccgtaa cccacggtgt attgctgggc tttggtcagc ggaacggttc
     7561 cgccggggcc ttccagcctc aggtccgacc attcgaagcc gtcgctgccg atgccgttgc
     7621 cgccgtcatc cacgcgcagg cgcacctgct ggccgccctt taccggaatg gaaagggaaa
     7681 cgggcttttt agcctgttcc tcccggaaga cttccttcca gagaggctgg atgccctgga
     7741 ttttttccac gctttccgcg ggaatgaggt tcagggtgat ttttccggct ccttccgcct
     7801 tgtccaccag aaggcccgcg tgcatgtcca ggccgcgcct gttctgatgc tgataagggc
     7861 cgtaaagcac gtccaggctt tgcgacgttt tgaagaggtg tccgtcaatg cccaggatca
     7921 ggtcaccttc tttgacgtat ttttcggcag gggaaccctc aatcacctgg gtgaccgtgt
     7981 aannnnnnnn nnnnnnnnnn nnnnnnnnnt cgccacgggt tccccgttgg ggtccagcat
     8041 ggttttgggg aagaagccgc ggaagttcgt gtccggccag gaggcgtcca ggccgcgggc
     8101 gcggaagccc agagggccga aggtggtgat ccagcctttg gctacttcct cggaattgcc
     8161 gaaccagctc gtgtacagct cggcgtattg ttttctccac tggttcagcg gcatgggctt
     8221 ggcgaatttt ttcacatagg cgttcacatc ccccatattg gtcgtttgcc tggcggcttt
     8281 cccgcgcgga cgcactacgg gcgcaggttt ggcggcagtc tgtccctcct gtgattcctc
     8341 ccggttggcg ggctgttgcg gttccggctg ttttccgtcc gcagcgtcat ctgccggggc
     8401 ggaggagttt tcgtcaggct gtgcatgggt ggctgcggat ttttcctcca aagcgcccgg
     8461 ggcatcttca ttccgttccc cggaggaagt ggccggcggc gttttttccg gctgctccgc
     8521 cagtccggac ggggagaggc ccgcggcgtt ttcctgttcg ggggaggctt ctcccctcac
     8581 ggcgggcggg gcggctttct tttccctgtt ggagtgggga acgtccggct cctgagcaga
     8641 gacgggtttt tccgcggggt tccgggtgaa ataataggtt ccgcatgctc ctcccagcag
     8701 gagcagggtg agaatccata tgccggcgga gttcttcttt ttccgcagct ggggaaggga
     8761 gcccgcatga acgggcgtcg ccccggttcc gggagtggtc aaggtacgct gatcacg
//
LOCUS       DAOMSU010000073         8808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_565_length_8808_cov_3.032789, whole genome shotgun sequence.
ACCESSION   DAOMSU010000073 DAOMSU010000000
VERSION     DAOMSU010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8808
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_565_length_8808_cov_3.032789"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="PK077_04620"
     CDS             complement(<1..297)
                     /locus_tag="PK077_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN23615.1"
                     /translation="MLTIKNLTKSHAGRTLFRETEMTINWGERVALVGPNGAGKSTLF
                     RMILGEDAPDEGSISMDEYAIVGYLPQEASEPKDETVLEIALGITPEMEQAIHTI"
     gene            525..1127
                     /locus_tag="PK077_04625"
     CDS             525..1127
                     /locus_tag="PK077_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23616.1"
                     /translation="MQQQMSGLANAFLALLPPDQATLPAAGAEARWLADTAVVQSAAI
                     ARDNRTVLFGWLNNILVNSNLRDRGLCWQVQQDLYRDLRRRPVKYFRIGLTIRDRGTG
                     REHSCVYVNAAGKGLQGSIVLDAWKNCGHLVTLTQKDREGGKWEEDWREPFVSKAFPE
                     GHSYGMEHHLVWPGWARKRPILVQRIFQSDQQNEAKAAAE"
     gene            complement(1337..1465)
                     /locus_tag="PK077_04630"
     CDS             complement(1337..1465)
                     /locus_tag="PK077_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23617.1"
                     /translation="MKNIAILAIAGAACLVASCSQQQQQQQTTAPAPAPVVQAKGK"
     gene            complement(1679..2272)
                     /gene="raiA"
                     /locus_tag="PK077_04635"
     CDS             complement(1679..2272)
                     /gene="raiA"
                     /locus_tag="PK077_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRN23618.1"
                     /translation="MQKVNVNTPITITGRHVEVTDAIREFVTKKIEGIRLDFPRIMEV
                     KVLLDVQRDRKISQVILFCSDHITIDASTEGTDMYACIDETITKIMRRMRKHKTRLMK
                     NFRPHHQETIRKLDETVYDDSVLDYPGDSQEDPEPLLIHRESYNLKKLYKEEAIMELE
                     LSDKPFVLYRNARRDVLQIVYRRPDGDYSIIELGSSL"
     gene            2621..2697
                     /locus_tag="PK077_04640"
     tRNA            2621..2697
                     /locus_tag="PK077_04640"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2655..2657,aa:Met,seq:cat)
     gene            2906..3154
                     /locus_tag="PK077_04645"
     CDS             2906..3154
                     /locus_tag="PK077_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23619.1"
                     /translation="MGAFKSTVLVCNLLGVRNTTDRVTEEEIRLMIQESARDGDVQEV
                     ERIFNLGDRDLESIMTPRGGGHIAWQDAKMTEQPCGQG"
     gene            complement(3300..4169)
                     /locus_tag="PK077_04650"
     CDS             complement(3300..4169)
                     /locus_tag="PK077_04650"
                     /inference="COORDINATES: protein motif:HMM:NF005395.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRN23620.1"
                     /translation="MILTGAGQIGMAIARRIGSGMKIVIGDKSVENANAMARTMNQAG
                     FDATPLEMDLSSRNSILHLIAEAQGYGNISMLVNAAGVSPSQASVETILQVDLYGTAV
                     LLEEVGKVIMPGGTGVTISSQSGHRMPALTPEQDEQLAVTPAEELLALPLLHPEQIKD
                     TLHAYQMAKRCNVKRVMAEAVKWGARGARVNSISPGIVVTPLALDEFNGPRGEFYKNM
                     FAKCPAGRPGTADEIAHVAELLMGGKGSFITGADFLIDGGATASYFYGPLKPHDSKEK
                     TSQTSENGRQSTK"
     gene            complement(4375..5004)
                     /locus_tag="PK077_04655"
     CDS             complement(4375..5004)
                     /locus_tag="PK077_04655"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRN23621.1"
                     /translation="MKTWLFTSLLMSTLVLPGSCKDAKEEKNLPSGKPLVVYFSATGN
                     TKAVAEQIAKLTGADICRIEAAEAYNTDPYHDSDRVKKEAYEDLRPKVANLPEASLMA
                     KYDTIFVGSPIWWHQPAMVICTFLDAFDLKGKTIIPFFTYDATTYLNESMQKIYRLTP
                     HSRHIPATLPEDLDPGDITTPGRTDDEGIDMPGSAAGVKTWLKKINMLP"
     gene            complement(5110..5952)
                     /locus_tag="PK077_04660"
     CDS             complement(5110..5952)
                     /locus_tag="PK077_04660"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRN23622.1"
                     /translation="MKLSSVVLCCLMSSVSALTANSSPSSQELTFRQKHIVAIAAYTG
                     QGNLHQLRPALNAALDSGLTINEIREVLVHSYAYCGFPRSIRGLQAFISVLDKRKSRG
                     ITDTPGRDASPLKDKRSRYNRGCAILAEISGVPANAPKAAYAEFAPVMERFLKEHLFA
                     DIFERDVLTYDERELATVSILAAIGGVEPMARSHMGICLNLGITPGQLCQLLDIVSRN
                     IGPGEADAVRKELNTLLQERGLPGVSGTGQVAGKRETPANPPLRRRRENPAKHRSLAW
                     NRSR"
     gene            complement(5971..7104)
                     /locus_tag="PK077_04665"
     CDS             complement(5971..7104)
                     /locus_tag="PK077_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRN23623.1"
                     /translation="MKTKAIISIALSLASFNSPLNAQQMNTNDSPAVSTAHSAKSPFT
                     EVKGIKSHNGNPWGLVYRGAITENAEGRVNIHPITYMLNGLQIAANVYTPAGYDASKK
                     YPALVVAHPNGGVKEQVAGLYSQRMAEQGYICLAFDAAYQGASGGTPRQTDKPANRME
                     DIRRAADILLQYPGVDASRLGILGICGGGGYTLKAAQTDKRFKAVATLSMFNTGLVRR
                     NGFLDSQINSVQERLSDACAARQREAEGGQPEYVGDMKGVTPEQAAKLPFDLYRDGYG
                     YYLQSHAHPNSTFRYTKSSLIDLFAFDASEGMHLINQPLLMMAGSLADTFYMTEQCYS
                     RATGTRNKELYLIPGATHIKTYYVPEYVDQAVGKLAEFFGENL"
     gene            complement(7243..8358)
                     /locus_tag="PK077_04670"
     CDS             complement(7243..8358)
                     /locus_tag="PK077_04670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRN23624.1"
                     /translation="MMKIPQLILPAFFLSIFTPPAMIQAEDSTPVPAAEQAPLLIARQ
                     GSFAVGGSILQRPGTYDNSRFPGWGNAVEAGQSYHADHAAVDFQIPARARELPLVFIH
                     GYGQSARCWQTTPDGREGFNNIFLRKRYAICLVDLPGKGRAGRTTAETAVKPLADEQF
                     WFDIFRIGEWPTFNPGVQFPTDKDSLDQFFRQMTPDIGTHDMEKELDALSALFKRVGK
                     GILVTHSAGGFPGWLTAIRSPEVRGIISLEPGTYVFPEGEAPEDMPSLTGTMKGIAVP
                     PEEFNKLTQIPIILYFGDYIPENVTDKLGGENWRVRLQMGRKFVEAVNRHGGNAVLVE
                     LPKIGIRGNTHFLMSDKNNNIIAGHMAAWLRQNGLDR"
     gene            complement(8418..>8808)
                     /locus_tag="PK077_04675"
     CDS             complement(8418..>8808)
                     /locus_tag="PK077_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRN23625.1"
                     /translation="FSPLANGLLSGRYNLNSTFEPGTDYRSSMPQFRPESFDANKDLF
                     LLLRRLAEEKQAAPAQISLAWMLCKKPWIVPIPGTRKFSRLKENAEAAHVILSADEVR
                     QIDAALSFMNMSDVFGGSPIKEKSANR"
BASE COUNT         1787 a         2188 c         2604 g         2229 t
ORIGIN      
        1 gatggtgtgg atggcctgtt ccatttccgg cgtgatgccc agggcgattt ccagaacggt
       61 ttcatccttc ggttcgctgg cttcctgggg aagatagccc acgatggcgt attcatccat
      121 gctgatggag ccttcgtccg gcgcgtcttc tcccagaatc atgcggaaca gggtggattt
      181 gcctgctccg ttggggccaa ccaaagccac ccgttccccc cagttaatgg tcatttccgt
      241 ttccctgaac aaggtccgtc cggcgtggga ttttgtgaga tttttgatcg tcagcatggt
      301 gaagagagga tgtattctac cgggatggaa aggggaaggc aagagccgtc agggcggagg
      361 caggcatttt tgaaagcttt actggatttt tacgcgtagt ctgctagcct tggcgcgcca
      421 tgccgaccac gcagacattc aggcttttgc ttgctgccgc ccttttgtgc ggatggagtt
      481 cctgctgtac tcctccagcg gaggattata tacaacaacc ttacgtgcag cagcaaatgt
      541 ccgggctggc taatgccttc ctggcgctgc tgccgccgga tcaggccact cttcccgctg
      601 ccggagcgga ggccaggtgg ctggcggaca cggccgtggt ccagtccgcc gcgattgccc
      661 gggacaacag gacggtgctg tttggctggc tgaacaatat tctggtcaat tccaacctgc
      721 gcgacagggg gttgtgctgg caggtccagc aggatttgta ccgggacctg cgccgccggc
      781 ccgtgaagta tttccgcata gggctgacga ttcgagaccg tggaacgggc agggagcaca
      841 gttgcgtgta tgtcaatgcc gcagggaagg gattgcaggg ctccattgtt ctggatgcct
      901 ggaagaactg cgggcatctg gtgacgctga cccagaagga ccgggaaggc ggcaaatggg
      961 aggaggactg gcgggaacca tttgtttcca aggcctttcc ggagggccat tcctacggca
     1021 tggaacatca cctggtctgg cccgggtggg ccaggaaacg ccccattctg gttcagcgga
     1081 tattccagtc cgatcagcag aatgaggcga aggcggccgc tgaataaagg ggaagcctga
     1141 tccggcagga accggtacat ggtgttgcgt ggcggaagtt tgcggtatcc gccgttttta
     1201 aggaaaatcc cggcgaatcg gcaaatggcg gagggagccc ggattcaggg caaataaaaa
     1261 cgcactcagt gcgtacaccg agtgcggatt aattggatga tttgctagtg ttgcagggca
     1321 aaaccccgct gcaaacttac ttgcccttgg cctgcacaac cggagcggga gcaggagcgg
     1381 tggtttgctg ttgctgctgc tgctgggagc aggaagcaac caggcaagcg gcgccggcga
     1441 tggcgaggat agcgatgttc ttcataattc gaattaaata cagtgtttgt taactactgg
     1501 aacctccgca tccacggagg tttgctggca ccaatgtaaa tggtggcaag tagaaatcaa
     1561 gagattttct tgttccatgt cacggcatta cagaaaagag caggacctcc ggtttctgaa
     1621 gtcctgctct ttttgatgcg ttatggaaaa cggattgttt caaaaccgtt ttttgcgctt
     1681 acaggctgct cccaagctca atgatggagt agtccccgtc cggcctccgg tacacgatct
     1741 ggagcacgtc gcgccgcgca ttcctgtaga gtacgaatgg cttatcggaa agttccagtt
     1801 ccatgatggc ttcttccttg taaagctttt tgagattgta gctttctctg tgaatgagga
     1861 ggggttccgg gtcttcctgg gaatctccgg ggtaatccag cacggaatcg tcatataccg
     1921 tttcatccag cttgcggatg gtttcctggt gatggggacg gaaattcttc atcaggcgcg
     1981 ttttgtgctt gcgcatgcgg cgcatgattt tggtgattgt ctcatcaatg caggcgtaca
     2041 tgtccgtccc ttccgtggac gcgtcaatgg tgatatggtc cgagcagaac aggattacct
     2101 gggaaatctt gcggtcgcgt tgcacatcaa gcagtacttt aacctccata atgcgcggga
     2161 aatccaggcg gatcccctca atttttttcg ttacgaattc ccgaatcgcg tctgtgactt
     2221 ccacgtggcg ccccgtgatc gtaatgggtg tgtttacgtt taccttttgc attgtcgtac
     2281 ctctttctag ttgatgttaa tgcgcggacc ccgtccgcac ctttcaagat atacagattt
     2341 gacaatgttt gtcgagaaat cctctctttt tttcgtgtgc agggaaaggc gtttttccgt
     2401 ttccgttccc atttgccgga accgggcggc tctcctacct gttgtgagcc aatcaatcgg
     2461 ggagcggctg tttccgttta ttgccttcct gcatctccgg aatgcgtttc agggcctctg
     2521 tcatacaact gcaggcatga aaaaattatt tgaaagtact tgacatgctg cggaaatttc
     2581 ttataacagt tgcgcactcc tctacagatg ctctctaaat cgcgggatgg agcagccagg
     2641 tagctcgtca ggctcataac ctgaaggtcg caggttcaaa tcctgctccc gtaaccattt
     2701 aaaaaccctg caagcaatga gcttgcaggg ttttttcatt acggatgaaa aagcatccgg
     2761 agggtattgg gagtctgttg tttctgggag cttttgtatt cggggaactg gtgtccaaaa
     2821 gggttggaat gtgtgctccg gaagtaattt ccaaattggg ggctgtgccg atgaaagcct
     2881 tttcttgtgc cgcagcaccc ttcatatggg tgctttcaaa agcacagttc tcgtatgcaa
     2941 tttgctgggc gtcaggaata cgactgacag ggtgacggaa gaggaaatcc gcctgatgat
     3001 ccaggaaagc gcccgggacg gggatgtaca ggaggtggaa cgtatattta atctcggcga
     3061 ccgggatttg gagtccatca tgactccgcg ggggggggga catattgcat ggcaggatgc
     3121 caagatgacg gaacagccat gcggtcaggg gtagaggtcc gttatccgtt ttggtcctta
     3181 ttataaatgg gtaacatgag aaacatcgcc agcaacgtgc gctgatcgtt ttttcatgtt
     3241 gttttgttgg gatgatctac ggtgtttccc ttttgatgct gcgttgattt cagtaagctt
     3301 cattttgtgc tttgtctgcc gttttcgctt gtctgagagg ttttctcttt tgaatcgtgc
     3361 ggtttcaacg gaccgtagaa gtaggaggcg gtggcgcccc cgtcaatcag gaagtccgcg
     3421 ccggtgatga aagatccctt gccgcccatc agcagttctg ctacatgggc tatttcatcc
     3481 gctgtaccgg gccggcctgc cggacatttg gcgaacatat ttttgtaaaa ttcgccgcgc
     3541 gggccattga actcatcaag agccagaggg gtgactacaa tgcccgggga aatggagttg
     3601 acgcgcgcgc ccctggcacc ccatttaacg gcttccgcca tgacgcgctt gacgttgcag
     3661 cgtttcgcca tctgataggc atgcagggtg tccttaatct gctccgggtg gagcaggggg
     3721 agcgcaagca gttcttccgc aggggtgacg gccagctgtt cgtcctgttc cggggtgagc
     3781 gccggcatgc ggtgcccaga ctggctggaa atagttaccc cggttccacc gggcataatg
     3841 actttcccca cctcttccaa caatacggcg gttccgtaga gatccacttg aagaatggtt
     3901 tcaacggatg cctggctggg ggaaaccccc gccgcattga cgagcatgga aatgttgccg
     3961 tacccttgtg cttctgcaat caaatgcagg atggagtttc gggaggagag gtccatttct
     4021 aagggggtgg cgtcaaaacc ggcttgattc attgtccggg ccatggcatt cgcgttctcg
     4081 acgcttttat cgccaatgac gattttcatg ccgctgccta ttctgcgggc aatggccatg
     4141 ccgatttgcc cggctccggt taatatcatg acttctttgg ctggacgttg ttccatgggc
     4201 gtgtgaaatt agttgaaatg gccggttttt attcccggca gcatgttata tgttcctctg
     4261 accggacagt atggcccttc cgcaaaaagg acaattcaaa aacagccata ggggtggtat
     4321 tgcaacgttt tcatatggat ggaattgagg gggatgaaag aacccgggca aaaatcatgg
     4381 cagcatgttg atttttttca gccatgtttt gacgcctgcg gcgctgcccg gcatgtcgat
     4441 tccctcgtca tcggtgcgtc cgggagttgt aatatctcct ggatcaaggt cttctggaag
     4501 ggttgccgga atgtgacggg aatgaggcgt aagcctgtaa attttttgca tggattcgtt
     4561 taaataagta gtcgcgtcgt aggtaaagaa ggggatgatc gtttttcctt tcaggtcaaa
     4621 tgcgtccaaa aaggtgcaaa tgaccatagc gggctgatgc caccagattg gagagccaac
     4681 gaagatagta tcatatttcg ccatcaggga ggcttcaggc aagttggcga ccttggggcg
     4741 caggtcctca taagcttctt ttttgacgcg gtcgctgtcg tggtaagggt cggtattgta
     4801 ggcttcggcg gcttcaatcc ggcagatatc cgctccggtc agtttcgcga tttgttccgc
     4861 caccgctttg gtattgcccg ttgcggagaa atatacaacc agcggttttc ctgaaggaag
     4921 gtttttttct tcctttgcat ccttgcagga accgggtaag accagcgttg acattaataa
     4981 tgaagtgaac agccaggttt tcatctattt ttattgtggt aattgattat acttttttct
     5041 gttgccgcgc tgagccggac ggctccgctt tggctttgcc ggagaccgat ggcggtatgg
     5101 ataaattcgc tatcgggagc ggttccatgc caatgaacga tgtttggccg gattttcacg
     5161 acgtctcctg agcggaggat ttgcgggtgt ttcccttttt cccgcaactt gtccagttcc
     5221 ggaaacacca ggcagccctc tttcctgcaa cagcgtattc agttctttcc ggactgcgtc
     5281 tgcttcaccg gggccgatgt tccgggatac gatatcaagc aactggcaca gttgtccggg
     5341 tgttatgccc aggttcaggc agatgcccat gtgggagcgg gccatcggtt ccacaccgcc
     5401 tatggcggca aggatggaaa cggtggccaa ttcgcgttca tcataagtca gaacgtcccg
     5461 ttcaaaaata tctgcaaaca ggtgctcttt caggaaacgt tccatgaccg gggcaaattc
     5521 ggcataggcg gctttcggcg cattggcggg aacgccggag atttccgcaa gaatggcgca
     5581 gccgcgattg tagcggctgc gcttgtcttt caagggggag gcatctcggc ccggtgtgtc
     5641 ggttatgccg cgggactttc ttttatccag tacggagatg aatgcctgca acccccttat
     5701 gctccgcgga aatccgcaat aggcatagga atgcaccaag acctcccgga tttcatttat
     5761 cgtcaatcca ctgtccaagg cggcattcag agccggccgc agttgatgca aattgccttg
     5821 gcctgtatag gcggcaatgg cgacgatgtg tttctgcctg aaagtcaatt cctggctgga
     5881 aggggatgaa ttggccgtca gggccgacac ggacgacatc aggcagcaca ggacaacaga
     5941 cgagagtttc atgaacctgg gagaagggtt ttacaggttt tccccgaaga attccgccag
     6001 cttcccgaca gcctgatcca cgtattccgg cacgtaatag gtcttaatgt gcgtggctcc
     6061 ggggatcagg tacaattcct tgttccgggt tccggtggcg cggctgtagc attgttccgt
     6121 catgtagaag gtgtcggcaa ggctgcccgc catcatcagc aggggctggt tgatcaggtg
     6181 cattccttcg ctcgcgtcaa aggcaaagag gtcgatcagg ctgcttttag tgtaacggaa
     6241 agtggaattg gggtgggcgt ggctttgcag ataatagccg tagccgtccc ggtagaggtc
     6301 aaagggcagt ttggccgcct gttccggcgt tactcctttc atgtccccca cgtattccgg
     6361 ctgaccgcct tccgcttcgc gctggcgggc ggcgcaggcg tcagagaggc gttcctgcac
     6421 gctgttgatc tgcgagtcga ggaacccgtt gcggcggacc aggccggtgt tgaacatgct
     6481 gagcgtagcg acggccttga agcgtttatc cgtttgtgcg gctttcaggg tgtagcctcc
     6541 gccgccgcaa atgcccagaa tgccaagacg agaggcgtcc actccgggat attgcagcag
     6601 aatgtccgca gcgcggcgga tgtcttccat gcggttggcg ggtttatccg tctggcgggg
     6661 agtacctcca ctggcgccct gataagccgc gtcaaatgcc aggcagatgt agccctgttc
     6721 cgccatgcgc tggctgtaaa gcccggccac ctgctccttc acgcctccgt tgggatgcgc
     6781 cacgacaagg gcggggtatt tttttgaggc atcatatccg gcaggcgtat aaacattggc
     6841 ggcaatttgc agtccgttca gcatataggt gatcggatgg atgttcacgc ggccttccgc
     6901 gttttccgtg atggctcccc ggtaaaccag cccccaggga ttaccgttgt ggcttttaat
     6961 cccttttact tccgtaaatg gggatttggc ggaatgggct gttgagacgg ccggagaatc
     7021 atttgtattc atttgctgtg cgtttaatgg tgagttgaag gaagccaggg aaagggcgat
     7081 tgaaataatg gcttttgttt tcatggcatg tactttggtt tttcaagcag ttcccattct
     7141 atcatgaaag actctcttcc cgtcgataga cggattacag gttttcttac catgattacg
     7201 gatggcgacc gcagaaagta aatgtgcggg ggaggatgtc tgctacctgt caaggccgtt
     7261 ttgccggagc caggcggcca tatggccggc aatgatgtta ttgtttttgt ctgacatcag
     7321 aaaatgcgta tttccccgga tgccgatttt gggcagttcc accagaacag cattgccgcc
     7381 atgcctgttg acggcctcca caaatttgcg ccccatttgc aggcgcacgc gccagttttc
     7441 ccctccaagc ttatcggtga cgttttcggg aatatagtct ccgaagtaga ggatgatggg
     7501 gatttgggtg agcttgttga attcctccgg cggaacggcg attcccttca tcgtgccggt
     7561 caggctgggc atgtcttcgg gggcctcccc ttccggaaag acgtaagtgc ccggttccag
     7621 ggaaatgatg ccgcgcactt ccgggctgcg gatggctgtc agccagccgg ggaatccccc
     7681 tgcggagtgg gtaaccagaa ttcccttgcc cacgcgcttg aagagggcgc tcagggcgtc
     7741 gagttctttt tccatatcgt gcgtgccgat atcaggcgtc atttggcgga aaaactggtc
     7801 cagggaatcc ttgtccgtag ggaactgtac tcccgggttg aatgtcggcc attcgccgat
     7861 ccggaagatg tcaaaccaga actgctcgtc ggcaagcggc ttgacggcgg tttccgccgt
     7921 cgttctgccc gcccggccct tcccggggag gtcaaccaag cagatggcgt atcttttgcg
     7981 caggaaaatg ttattgaagc cctcccgtcc gtccggagtt gtctgccagc accgggcgga
     8041 ctgtccatag ccgtggataa ataccagagg cagttcccgg gccctggccg gtatctggaa
     8101 gtccacggcg gcgtggtcgg catggtagct ctgaccggct tccactgcgt tgccccagcc
     8161 gggaaaacgg ctgttgtcat acgttcccgg gcgttgcaga atgctcccgc ccacagcaaa
     8221 gcttccctgc ctcgcaataa gcaggggggc ttgttccgcg gcgggaacag gagtgctgtc
     8281 ttccgcctgg atcatggccg gaggcgtgaa tattgataag aaaaaggcag ggaggattaa
     8341 ttggggtatt ttcatcatgt catgaagggg attgagctgt catgtttccg caagggaaga
     8401 aaagcgtgat ttgctgatta acggtttgct gatttttctt tgatggggct tcctccaaac
     8461 acgtcggaca tgttcatgaa ggaaagcgcg gcgtcgatct gccggacttc atccgcggac
     8521 aggattacat gggctgcctc tgcgttttct ttcagtctgg agaactttcg tgttcccgga
     8581 atgggaacga tccagggctt tttgcagagc atccacgcca gggaaatttg tgccggagca
     8641 gcctgttttt cttccgccag cctgcggagc aggaggaaga gatccttgtt ggcgtcaaag
     8701 ctttccggtc tgaactgagg catggagctg cggtagtccg tccccggctc aaacgtggaa
     8761 tttaggttgt agcggccgga gagcaggccg ttggccaggg gggagaac
//
LOCUS       DAOMSU010000074         8600 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_586_length_8600_cov_3.190404, whole genome shotgun sequence.
ACCESSION   DAOMSU010000074 DAOMSU010000000
VERSION     DAOMSU010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8600
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_586_length_8600_cov_3.190404"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="PK077_04680"
     CDS             complement(<1..1362)
                     /locus_tag="PK077_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23626.1"
                     /translation="MNNYIPLNKAVGPKLICDGEQALIPQKLGVDAMTFNFDGSACEH
                     ATCAGAPEMFIKVEEDGLYYFGVEADDTGSLTIAGEQAIKKDGTPPNGKLNIETDSRY
                     LKAGYYKVALSWTNNAYTPVSNNAIAFNVTMDTKPITEGKYEGNSTMKREFSSSPKIK
                     LWTIEKESTITCEPSKEVELEFEKPEPVYLEGNGDCNVSSLRPKICVTVCKDESENVW
                     RCRVVSVSAGAKLTMYEGIYVNPYVDPPLNEEEATEAVNEMNGYQSRAEVGTWHTPQA
                     SLAHEEHHRRQWEDAYKFYWKDSKIQEMLEKQTISCDKEPNMDKAVKFMQALANEMAL
                     DLWKETSDYVLALPDDANDRPYCAGQEVLNEATAYVIRLADAMGWNNVPRFITKPGTI
                     EPPCFMPPVSEGETRSMAVAEEPAPLILSMADTSQFTEGKITVRFRNEGNEPVRIPDE
                     INDE"
     gene            complement(1774..2181)
                     /locus_tag="PK077_04685"
     CDS             complement(1774..2181)
                     /locus_tag="PK077_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23627.1"
                     /translation="MNEQDDTKLWQILGHASQPAPGDDFARKVMMRIEREECAPAVPV
                     ESIHHFKRHSFRIWGAAAAALVAAVIGIAALMEPSAPETVPLSIATLNIDDVLVEEAG
                     LALGQENLVDALCVLSSTETGVISSDNIQDLLL"
     gene            complement(2249..3214)
                     /locus_tag="PK077_04690"
     CDS             complement(2249..3214)
                     /locus_tag="PK077_04690"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRN23628.1"
                     /translation="MIESSPPPGIRASLNEAGELVLTAVNAPPEAVIRMDVNAGSPRM
                     LCTQGRYLAPVQAPPGARIRFRLFRGKRGITAPETFIMPGPPPARAVPSTLIPCTQDR
                     DFMIYDWASRHEAACRIVRETHPDLLFIGDSITHFWGGAPVDEPHRDILQKSPETWNL
                     CTAGMRAVNLGFGYDRVENALWRLRHGELDGAEDNAVCVVLLGTNNLAENTDGEILEG
                     IRAVCREITGKLAKAVIILQGFYPRNSAREGTAERIVGINLLLNRLATEENFIYTEPG
                     RVMANSDGYVPEELSSDGLHPSAAGYARIASVLAPVIRQAAEREK"
     gene            complement(3211..4284)
                     /locus_tag="PK077_04695"
     CDS             complement(3211..4284)
                     /locus_tag="PK077_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23629.1"
                     /translation="MNPLLFPCLLAAAIFPAPAQELPAQELPALKWTNHDQQSLMRGA
                     WWNANPPLLPPVDAAAAPSDPMPEPAANADSPAVDPIPEDFTDVNEYFLPDYIRTSTG
                     LIDPQKLLAEVESNDVIELIKLIKSRYNVNLFVSIFAAGQKVPPSVNAPTIARQIFRN
                     KERNLLLHFHMGDIKSAQIALDPELSSQLRDAGRRDLLYQVKQDASRFTNPQDELIEA
                     MISLARRTEADMASAPRPTLPALEPDNPASIPEANITIQEPEETQESSLHAMMKAWMT
                     FALANVAGIILHISALAAIVVLWLVWKNKRVVHLLPSEPDKRLGAPHGASQSRPVNYC
                     MQDSRGPNSIGRRQMRQHMRDVS"
     gene            complement(4281..4850)
                     /locus_tag="PK077_04700"
     CDS             complement(4281..4850)
                     /locus_tag="PK077_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23630.1"
                     /translation="MCPHCGFSLENLDKKYGKDAIPYRRVCDNAGALRQQDRMRLNAL
                     LEKLERRIPPVLLSVYFPNILEPFSLIPHSFWTMNHLTVDEAGFPNHQGPLDPQWLLV
                     LVLDVRTDTACFMWGYELDPYVEPDLINKSIMKARIPLRESMLLQAAGAIMKNAVQLV
                     ARKARSKVRHPMRYGLTPPTPEDRKGGGA"
     gene            complement(4968..5741)
                     /locus_tag="PK077_04705"
     CDS             complement(4968..5741)
                     /locus_tag="PK077_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HRN23631.1"
                     /translation="MSEELVEEFITVESIFVRGRNCLALRSKFSPLFTDYYLHLMQYG
                     LRNEELCDSLLKELMAYFTLYMVARPWAEQHAWTVNLRTPAVANLFVTGSSLTESVVG
                     RVFTQDIKEPEENTLYSTLLRKGFEPQYSVVPIPGTSPARWVEEFYRQSEQRNARCIE
                     LPDECYTMVTAQPDADEEWLASLTREKMAHLEEDENTRVLETRRFRFYCGCTLDRILP
                     TLKTLQDNKEDLFQGEEELEVTCPRCAAIYRVTKENLED"
     gene            5944..6447
                     /locus_tag="PK077_04710"
     CDS             5944..6447
                     /locus_tag="PK077_04710"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRN23632.1"
                     /translation="MATDKNITIHTSKGDIRLTVFASKTPVTAASFLNLASRGFYDGL
                     KFHRVIPDFMIQGGDPTGTGMGGPGYRFEDECRPDLRHDGPGVLSMANAGPGTNGSQF
                     FITHVPTDWLNGKHTVFGKVTEGQSVVDSIKQGDTISGITIEDNADDLFAEQADRIAA
                     WNKALGK"
     gene            6528..>7934
                     /locus_tag="PK077_04715"
     CDS             6528..>7934
                     /locus_tag="PK077_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRN23633.1"
                     /translation="MVLAVQNLRVQYGARVLFNDLSFTVEDGERIALAGHNGAGKSTL
                     MKCIAGLNEPDSGSIIKSRHSQVGYLPQEGIHIRGRRLIDEAMSAFADLMDLQERIDA
                     LTREMAGLDPRSAAYSEVLNEIGELELVLHAHNSARLRPRTESILRGLGFKDRDFDRD
                     CGEFSGGWQMRIALAKLLLREPEVLLLDEPTNHLDIQSQRWMEQYLRSYRGAIILISH
                     DVALLDALVSRTIAFYHGRAEEYAGNFSYFLKESVLRKEILLRQKKAQEREIAKTKEF
                     IDRFRYKATKASLVQSRIKQLEKVELIEVEEDDAVMNFHFPAPPAGAHSVVRLEKVSK
                     HYGSVSVFENVDFEIVKGDRIAIVGVNGAGKSTFSRLISGGEAPSSGSVTMGRHTQVA
                     FFSQTHADDLDPEKTILECVEAAATRESAPMVRNLLGCFLFRGDDVHKRVGVLSGGER
                     SRVALVCMLLHPANFLILD"
     assembly_gap    7935..7944
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7945..>8600
                     /locus_tag="PK077_04720"
     CDS             <7945..>8600
                     /locus_tag="PK077_04720"
                     /inference="COORDINATES: protein motif:HMM:NF027650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN23634.1"
                     /translation="FQSLSGGEKQRVVLARALTQSPKILILDEPTNHLDIQSQQVLQQ
                     ALSEYPGSYCIVSHNRSFLDPIVTKVLEFVPGEKPRVYLGNVSDYLEKVERNQALAAA
                     AALPPSGAAEPGAGLDRKARRRMEAEIRQKKTRLLRPLQEKLELLEEEIARLETEKTE
                     ITSQLERPDVAADTEAVMELTSRFQQTDRQLETCFTQWADLSEKIEETEARIQEEAER
                     "
BASE COUNT         1754 a         2274 c         2549 g         2013 t
ORIGIN      
        1 ttcgtcgttg atttcgtccg gaatccggac aggctcgttt ccttcgttgc ggaagcggac
       61 tgtgattttg ccttccgtga attgggaagt atccgccatg gagaggatca agggcgccgg
      121 ttcctccgca acagccatgc tgcgagtttc accttcgctg accggaggca tgaaacaggg
      181 aggctcgatc gttccaggtt tcgtgataaa cctgggcaca ttgttccacc ccatcgcgtc
      241 ggccagacga atgacatagg cggttgcctc gttcaggact tcctgtccgg cgcaataggg
      301 tctgtcattg gcatcatccg gcaacgccag tacatagtct gacgtttctt tccaaaggtc
      361 caatgccatt tcattggcaa gagcctgcat gaacttcacg gctttatcca tgttcggttc
      421 tttgtcgcag gagatagtct gcttttcaag catttcctgt attttggagt ccttccagta
      481 gaacttgtag gcatcctccc attgacggcg gtggtgttct tcgtgggcga gggaagcctg
      541 cggcgtgtgc catgttccaa cttctgcacg agattgataa ccgttcattt cgttgaccgc
      601 ttcggtggct tcttcttcat tgagaggggg atcaacatag ggattcacgt aaatgccttc
      661 atacatagtt agcttggctc cggcagaaac ggaaacaaca cgacacctcc atacgttttc
      721 cgattcatct ttgcaaaccg ttacgcaaat cttaggtctc aagctactta cattgcagtc
      781 accattccct tccaaataga cgggttccgg tttctcaaat tccagttcca cttccttgga
      841 tggctcacag gtgatagtgg attccttttc aatcgtccac aatttgattt tgggggacga
      901 cgagaattcg cgcttcatcg tcgagttgcc ttcatacttc ccttccgtaa taggtttggt
      961 atccatggta acgttgaatg caatggcatt gttgctgacg ggggtgtagg cgttgttggt
     1021 ccacgacagg gccaccttgt aatagcccgc tttcaggtac ctggaatcgg tttcaatatt
     1081 cagcttgccg ttgggtggtg tcccatcctt ttttatagct tgctcaccgg caatcgtcag
     1141 gctgcctgtg tcgtcggctt ccacgccgaa ataatacagg ccgtcttcct cgaccttgat
     1201 gaacatttcc ggcgctcccg cgcaagtggc gtgctcgcac gccgaaccgt caaagttgaa
     1261 cgtcatcgcg tcaactccga gtttctgagg gatgagagcc tgctccccgt cgcagataag
     1321 cttcgggccg accgccttgt tcagtggtat ataattattc atgttcctga attgtttctt
     1381 gttcacagaa ctaaccattt agttctgtga acaccttgca ggaattccaa aaaaacaaca
     1441 acacgccccg gacgtgatga aggaaaaatc ctcgattatc tccaataaaa atggcggatg
     1501 ccatccagcg cccgccatat atatgttaat agtttggaac gtatcgctac gcttgtgtat
     1561 gaattaagct agaccctaca ggggctggta gccccgttcc tcctgccgga tgagttgccc
     1621 ctccgggata cagactcttt acatttccgt tttcggaaaa agcggggttt ctgaaatacg
     1681 gattcaaggc agtcgggaaa aataatacgg aaacgcccgg cacggaggct tgtgacgccg
     1741 gacgggcatt gaatggtatt tccgttcccg gagttacaga agaaggtcct ggatgttgtc
     1801 cgaggagatg acacccgttt ccgtggagga cagtacgcag agggcgtcta ccaggttttc
     1861 ctgccccaga gccagcccgg cttcttccac cagcacatcg tcaatattca gggtagcgat
     1921 ggaaaggggc accgtttccg gggcagaggg ttccatcagg gctgcaatgc cgatgacggc
     1981 agccaccagg gccgccgccg cagcccccca aatgcggaag gaatgccgct tgaagtgatg
     2041 aatggattcc accggaacgg cgggagcgca ttcttccctt tcaatacgca tcatgacctt
     2101 gcgggcaaaa tcgtcccccg gcgcaggttg ggaggcatgg cccagaatct gccagagttt
     2161 tgtatcgtcc tgttcgttca tggttgtggt tccgctgagg gttctatgaa gaaaaacacc
     2221 ggaagatggg tccggtttct tgccggtgtc atttttcccg ttctgccgcc tgtctgatga
     2281 cgggggccag tacggaggcg atgcgtgcgt aaccggccgc agaagggtgc agtccgtcgc
     2341 tggaaagctc ttccgggacg tatccgtcgc tattagccat gacgcgtccc ggttccgtat
     2401 aaatgaaatt ttcttccgtg gccaggcggt tcaggaggag attgatgccg acaatgcgtt
     2461 ccgccgtacc ttcccgggcg ctgttgcgcg ggtaaaatcc ctggaggatg atgacggctt
     2521 ttgccagttt gccggtgatt tcccggcaga cggcacgaat gccttccagg atttccccgt
     2581 ccgtgttttc cgcaaggttg ttcgtgccca ggagcaccac gcaaacggca ttgtcttccg
     2641 caccgtccag ttctccgtgg cggagccgcc acagggcgtt ttccacccgg tcgtagccga
     2701 accccagatt gaccgcccgc attcccgccg tgcacaggtt ccatgtttcc ggggattttt
     2761 gcaaaatgtc ccggtgcggt tcatccacgg gcgcacctcc ccagaaatgc gtgatggagt
     2821 ctccgatgaa gaggaggtcc gggtgtgttt cgcgcacgat gcggcatgcg gcttcatgcc
     2881 gggacgccca gtcgtaaatc atgaagtccc ggtcctgggt gcagggaatc agcgtggagg
     2941 gtacggcccg ggcgggagga ggtccgggca tgatgaaggt ttccggggct gtaatgccgc
     3001 gctttccccg gaacaggcgg aagcggatgc gtgcgccggg cggggcctgc accggagcca
     3061 gatagcggcc ctgcgtgcag agcatgcggg gagagcccgc attcacgtcc attcggatga
     3121 cggcttccgg cggagcgttc accgcggtaa gtaccagttc tcccgcctca ttcagggagg
     3181 cgcggattcc ggggggtggg gatgattcta tcatgagacg tcgcgcatgt gctggcgcat
     3241 ttgcctgcgt ccgatggagt tcggcccgcg gctgtcctgc atgcagtagt tcacggggcg
     3301 gctctgggag gcgccgtggg gagcgcccag gcgcttgtcc ggctctgacg gcagcagatg
     3361 caccactctc ttgtttttcc agaccagcca gagcaccact atggccgcca gtgcggagat
     3421 gtgcaggatg atgccggcta cattggccag ggcgaaagtc atccaggctt tcatcatggc
     3481 atggagggag gattcttgcg tttcctccgg ttcctggatg gtgatgttgg cttccgggat
     3541 gctggcaggg ttgtcaggct ccagtgcggg aagggtgggc ctgggggcgg atgccatgtc
     3601 cgcctccgtt cttcttgcca gggaaatcat ggcctcaatg agctcgtcct gcggattggt
     3661 gaatcgggag gcgtcctgct tcacctggta caggagatcc ctccgtcccg catccctaag
     3721 ctgggagctc agctccggat ccagtgcgat ctgggcgctt ttaatatccc ccatgtggaa
     3781 atggagcagc aggttgcgct ccttgttcct gaatatctgg cgggcgatgg tgggagcatt
     3841 gacggaaggg gggactttct gtccggccgc gaagatgctg acgaacaggt tgacattgta
     3901 gcgggatttg atgagcttga taagttcgat gacgtcattg ctttccactt ccgcgagaag
     3961 cttctggggg tcaatcaggc cggtggaggt acggatgtag tccggcagga aatattcatt
     4021 gacgtccgta aagtcctccg gaatgggatc cacggcgggg gagtctgcat tggcggcggg
     4081 ttccggcatg gggtcggagg gagccgctgc cgcatccaca gggggcagca agggagggtt
     4141 ggcattccac cacgcgcctc tcatcaggga ttgctggtcg tggttggtcc atttcagggc
     4201 gggtaattcc tgggcgggta attcctgggc gggagccgga aagatggccg ccgccagcag
     4261 gcatgggaac aaaagggggt tcatgcgcct cctcctttcc ggtcctcggg ggtgggaggc
     4321 gttaatccgt agcgcatggg atgcctgact ttggagcgcg ctttcctggc tacgagctgg
     4381 acggcgtttt tcatgatggc ccccgcagcc tggagcagca tgctttcccg caggggaatg
     4441 cgggctttca tgatggattt gttgatcagg tccggctcta cataagggtc cagttcatag
     4501 ccccacatga agcaggccgt gtccgtgcgc acgtccagca ccagaaccag gagccactgg
     4561 ggatccagcg gtccctgatg gttagggaag ccggcctcat ccaccgtcag gtggttcatc
     4621 gtccagaaac tgtgcggaat aagggaaaag ggctccagga tgttggggaa atagacggac
     4681 aggagaacgg gggggatgcg gcgttccagt ttctccagca gggcattcag cctcatgcgg
     4741 tcctgctgcc ggagagcgcc ggcgttgtcg catacgcggc ggtagggtat ggcgtctttg
     4801 ccgtattttt tgtccaggtt ttccagggaa aaaccgcagt gagggcacaa tgattccgcc
     4861 ccgcggtgga aattatgtaa acacttcgga caagtcggca caaggaaagt ttaagcacga
     4921 agttcgttgc cgaaaagact aattgtcaga aagaaatcat gtatgtctca atcttccaga
     4981 ttttctttcg tgacacggta aatggcggcg catctggggc acgtgacttc cagctcctct
     5041 tctccctgga acaggtcttc cttgttgtcc tgaagggtct tgagcgtggg caggatgcgg
     5101 tccagcgtgc agccgcagta aaagcggaac cggcgcgttt ccagcactcg ggtattttca
     5161 tcttcttcca gatgggccat tttctcccgg gtgagggaag caagccattc ttcatccgcg
     5221 tcaggctggg ccgtcaccat ggtgtagcat tcatccggga gctcaatgca gcgggcgttt
     5281 ctctgttcgc tttgcctgta gaattcctcc acccaccggg ccggagacgt tcccggaatg
     5341 ggtacgacgg agtattgcgg ttcaaacccc ttgcggagca gggtggaata cagcgtgttt
     5401 tcttcaggtt ccttaatgtc ctgtgtgaag acgcgcccca ctacggattc cgtcagggaa
     5461 gaaccggtga caaacagatt ggcgacggca ggcgtccgga gattgactgt ccaggcatgc
     5521 tgctccgccc agggccgtgc gaccatatag agggtgaaat aggccatgag ttctttcagc
     5581 aggctgtcgc agagttcttc gttgcgcagg ccgtactgca tgaggtgaag gtagtagtcc
     5641 gtaaacaggg gagaaaattt tgacctgagc gccaggcaat tgcgtccgcg gacaaagatg
     5701 gattcaaccg taatgaattc ttctaccagt tcttcgctca tgtcattcaa attcggtctt
     5761 cctttgtgga aaatcaaccg ttcctcctgt cattaggaac catcatgaca ggcaggtgcg
     5821 gcagtattct ttttcccggc agggaaaggt gcggcggaga aacgggcggg aaataacgga
     5881 ggggcccgga gactgtacgc aataagcctt taccatgcct ttttgcgcgt ttaacatgca
     5941 cccatggcaa ctgacaagaa tatcaccatc catacttcca agggcgacat caggctgacg
     6001 gtgtttgctt ccaagacgcc cgtgacggcg gcttcctttc tgaatctggc gtctaggggg
     6061 ttttatgacg ggctcaagtt tcaccgcgtg attccggatt tcatgattca gggcggcgac
     6121 cccaccggta caggcatggg gggccctggc tacaggtttg aggacgagtg ccgcccggac
     6181 ctcaggcatg acggccccgg cgttctttcc atggcgaacg cgggccccgg caccaacggt
     6241 tcccaattct tcatcaccca tgtgcccacc gactggctga atggtaagca caccgtcttc
     6301 ggcaaggtga cggaaggcca gagcgtggtg gattccatta agcagggaga caccatctcc
     6361 ggcattacga ttgaagataa tgcggatgat ctgtttgcgg aacaggccga ccgcattgcc
     6421 gcgtggaaca aggcgcttgg taaataacct cattccggca ttcattcagc ggagcgcggc
     6481 acggggctgt tcgttccgct gttttcttct ctctgctttt tcagtacatg gttctggctg
     6541 ttcaaaacct gcgcgtgcag tatggcgcac gcgtcctgtt caatgacctt tcctttacag
     6601 tggaggatgg ggaaagaatt gccctggcgg gccataacgg cgcgggaaaa tccacgctga
     6661 tgaagtgtat tgccggtttg aatgaaccgg attccggctc cattatcaaa tccaggcatt
     6721 cccaggtggg ttacctgccc caggaaggaa ttcatatccg cgggcgcaga ttgattgatg
     6781 aagccatgtc cgcctttgcg gacctgatgg atcttcagga gcgcattgat gccctgacgc
     6841 gggaaatggc ggggctggat ccccgttccg ccgcctactc tgaagttctc aatgaaatcg
     6901 gggaattgga actggtgctg catgcccata attccgcccg gctgcgtccc cggacggaat
     6961 ccatcctgcg cggcctggga ttcaaagaca gggattttga ccgggattgc ggagaatttt
     7021 ccggcggctg gcaaatgcgc atcgcattag ccaaactgct gctccgggag cctgaagtgc
     7081 tgcttctgga cgaacctacc aaccatttgg acatccagtc ccaacggtgg atggaacaat
     7141 atctgcgttc ctaccggggt gctatcatcc ttatttccca tgatgtggcg cttctggatg
     7201 ccctggtttc ccgtacgatt gctttttacc acggccgggc ggaggagtac gcgggcaatt
     7261 tttcctattt cctgaaggaa agcgtgctgc ggaaggaaat tctgctgcgc cagaaaaagg
     7321 ctcaggaacg ggagattgcc aaaacgaagg aatttatcga ccgtttccgt tataaagcca
     7381 ccaaggcttc cctggtgcag tcccgcatca agcagctgga aaaagtggaa ctgattgagg
     7441 tggaagagga tgacgccgtc atgaactttc actttcctgc tcctcccgct ggcgcccatt
     7501 ctgtggtcag gctggaaaag gtttccaaac attatggttc cgtttccgtt tttgagaatg
     7561 tggattttga aattgtcaag ggagaccgca ttgccattgt aggagtaaac ggggcgggga
     7621 aatccacatt ttcccgcctt atttccggcg gagaggcccc cagttccggc agcgtgacca
     7681 tgggacgcca tacgcaggta gcttttttct cccagacgca tgctgatgac ctggatccgg
     7741 agaaaacgat tctggaatgc gtggaagctg ccgccacccg ggaatctgcg cccatggtcc
     7801 ggaatctgct tggctgtttt ctgttccggg gggatgacgt acacaagcgc gttggcgtgc
     7861 tttccggggg tgagcgctcc cgtgtggcgc tggtgtgcat gctgttgcat ccggccaatt
     7921 ttctgattct ggatnnnnnn nnnnactttc agtctctctc gggcggtgaa aagcagcggg
     7981 tggttctagc ccgcgcgctg acccagtcgc ccaagatcct gatcctagac gaaccgacca
     8041 atcatctgga tatccagtct cagcaggtgc tacaacaggc tctgtccgaa tatccgggtt
     8101 cctactgcat cgtttcccac aaccgcagtt tcctggatcc catcgtgacg aaagtactgg
     8161 aattcgtccc gggagaaaaa ccgcgcgttt acctgggcaa tgtttctgat tatttggaaa
     8221 aggtggagag gaaccaggcc ctggctgccg cagccgcctt acccccctcc ggagcggcgg
     8281 aaccgggcgc cgggctggac cgcaaggcac gcaggcgcat ggaggcggaa atccgccaga
     8341 agaagacgcg cctgctgcgt cctcttcagg agaaactgga gctgctggaa gaagaaatag
     8401 cacggctgga aacggaaaag acggaaatta cttctcaatt ggaacgtccg gacgtggcgg
     8461 ctgatacgga agccgtcatg gagcttacct cccgttttca gcagacggac cggcagctgg
     8521 aaacctgttt tacgcaatgg gcagatcttt cggaaaagat tgaagagacg gaagcccgaa
     8581 ttcaagaaga ggcggaaaga
//
LOCUS       DAOMSU010000075         8497 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_602_length_8497_cov_2.858090, whole genome shotgun sequence.
ACCESSION   DAOMSU010000075 DAOMSU010000000
VERSION     DAOMSU010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_602_length_8497_cov_2.858090"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="PK077_04725"
     CDS             complement(<1..799)
                     /locus_tag="PK077_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase"
                     /protein_id="HRN23635.1"
                     /translation="MTKEGYTSNERIKEESDFLRGNISDLLHDGSITHFPSEEEFLLK
                     FHGITQQDNRDERLIRKKNGQERNWLFMLRLRLPGGRMTPSQWLAAETLSDQFGNGTL
                     KLTTRQAIQIHGVVKQDLKQTMKDIHHAAITTIAASGDATRNVMVSLHPEDSGIHETV
                     FNQARELSRKLEPQTHAYHEIWLDAKRIYSGAEEEPLYGPGYLPRKFKIAFTLPPEND
                     VDVYAQDLSFVAIEKEGKLLGYDILAGGGMGYAYGNPGSFPRLADIIG"
     assembly_gap    858..886
                     /estimated_length=29
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<887..1598)
                     /locus_tag="PK077_04730"
     CDS             complement(<887..1598)
                     /locus_tag="PK077_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRN23636.1"
                     /translation="MHPSIKNILQSSVFILLALAIWEYGSLSGWWRPFLFPAPSAISG
                     YLEEAIRDNSLWSATVVTIRRLLLGYLAGISIGIPAGLLSARFQLIRNTIGVLALGFQ
                     GLPSVCWVPLAILWFGQEEAAILFVVVMGTVWSVILATENGLANVPPIYARAARTMGA
                     GSAYTLLFVTLPASAPFIVSGMKQGWAFSWRSLMSAEIFVPILTGLGLGQLLHFGREL
                     NSMEQVMGIMFVIILIGLL"
     gene            complement(1583..2380)
                     /locus_tag="PK077_04735"
     CDS             complement(1583..2380)
                     /locus_tag="PK077_04735"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN23637.1"
                     /translation="MSRLSIRNVSKSYFSRKKSLLALSSANLDIGEGEFICLVGPSGC
                     GKTTLLNMIAGLIKSDTGSISYDGEIISGPGPDRTVVFQDFALFPWLNVLHNVEFGLR
                     FTEIPRAKRLQIALENLKSVGLDNFAHARIHELSGGMKQRVAIARALALRPRVLLMDE
                     PFAALDAMTREQLYADLQAICAGHGITVVFVTHNVREAVCLGDRVILFSSHPGRICGE
                     FCIRLPRPRQINDPGVARLASNITRALKSISRPAEEKQDAQTSCTLP"
     gene            complement(2373..3398)
                     /locus_tag="PK077_04740"
     CDS             complement(2373..3398)
                     /locus_tag="PK077_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRN23638.1"
                     /translation="MKILPLAVNTLLCVGALLSPAFLASCREASERQDQIRFGLFPNV
                     THVQGLVARHFSRTGEGWFEKRIFERTGKNISILWYAYNAGPSAMEAMFANSLDFTYV
                     GPAPAINAYSKSNGTLLQIVAGAVQGGSGLVVPSHSGACTQKDFQGKIIATPQLGNTQ
                     DIACRTWLALGGVTVTQTGGDASILPTPTPEQIPLLRQGKLDGSWTVEPWISRLEKEA
                     GGKLLFLEKDAVTTVLTAQKDFLEKQPDVAQAMIEAHRELTLWIIGHPQEAQKIVVEE
                     LRELTRSSIDPSLILHAWPRLVFTNKISEEKLHELAKDTVRTGFYKKLPHVEGIIRND
                     KNNPTHE"
     gene            complement(3433..5160)
                     /gene="cysN"
                     /locus_tag="PK077_04745"
     CDS             complement(3433..5160)
                     /gene="cysN"
                     /locus_tag="PK077_04745"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HRN23639.1"
                     /translation="MDIDSYLNEHENKSLLRVLTCGSVDDGKSTLIGRLLYDSKLIFD
                     DQLAELRKASEKNGTAGAGKIDYALLLDGLKAEREQGITIDVAYRYFTTPRRKFIIAD
                     CPGHEQYTRNMATGASTADAAIILIDARHGVLTQTKRHAFIASLLKIRHLIVAVNKMD
                     LLNYSEEKFRKIEEEFGSFTQQLNIPDVRFVPISAIEGENVTQTTGKTPWYQGDHLLS
                     ILETLDASDSRNLRDFRFPVQTVIRPNLDFRGFAGSITSGSIRRGDPIVTLPSFQNSR
                     IRRIVTPDGDLEEAFSPQSVVLELEDEIDISSGDMIVKKGNLPHIEDRLEARVIWMSE
                     KPLLPGGKYIIRHAGRNIQGRIAELQYDIDVNTLESRHATQLPLNHVGRIVLETASPL
                     FYDYYRDNRSGGALILIDPLNNVTAGAGMLRPSHIDKVPEKEQEQLPTFVSSDERMET
                     FGHGGKQIYVAGEDSDLARSFAKQLERELHRLKAHTYGLDFKAEGVWGRSAREIVNAS
                     GLLAEAGLMSIAALPGLPVLPRKAKGAYCIWLGNVVSAPETADRILPPAEANENTASL
                     LARTLYVEF"
     gene            complement(5206..6117)
                     /gene="cysD"
                     /locus_tag="PK077_04750"
     CDS             complement(5206..6117)
                     /gene="cysD"
                     /locus_tag="PK077_04750"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HRN23640.1"
                     /translation="MNSIYRLSQLKQLEAESIHIFRDAISQFENPVLLYSIGKDSSVL
                     VHLAKKAFYPGRLPFKLLHIDSTFKFREMIEFRDRFVQENDLDLIVHSNQEGIAAGVN
                     PFTYGSRKYTDIMKTQALIQALTAGKYDAVFGGARRDEEKSRAKERIFSFRDKFNQWD
                     PKNQRPELWNIYNGRINPGESVRIFPLSNWTELDIWQYIRLEKIQVVDLYFSKPRPIV
                     ERDGSLIMADDNRLPLKPGETPQMRTVRFRTLGCYPLTGAIESTARTVEEIVAEMLET
                     RLSERSSRIIDHDGDASMEQKKREGYF"
     gene            complement(6136..6819)
                     /locus_tag="PK077_04755"
     CDS             complement(6136..6819)
                     /locus_tag="PK077_04755"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HRN23641.1"
                     /translation="MNAHSPTATDTLRQAAAQYEGNLVFATSFGAEDQVLTHMISHLG
                     LNIPLATLDTGRLFPETYELWARTEEIYGIRIIPYFPVAEEVENMILDKGVNLFRNSM
                     EYRHECCNIRKLRPLERLLAGKKAWICGLRSTQSITRKGLHITENDEANGIIKINPLA
                     NWSEEDVWNYIRAYGVPYNPLHDAGFPSIGCACCTRSVKRTEDVRDGRWWWEKKEHRE
                     CGLHRKPRH"
     gene            complement(6876..7805)
                     /gene="cysK"
                     /locus_tag="PK077_04760"
     CDS             complement(6876..7805)
                     /gene="cysK"
                     /locus_tag="PK077_04760"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRN23642.1"
                     /translation="MNIYTSISELVGKTPLLEPRNYAQAHRLNARLLLKLDHLNPAGS
                     MKDRIALAMINEAEASGQLKPGAVIIEPTSGNTGIGLAAIAASRGYRIILTMPESMSV
                     ERRNILKAYGADIVLTEDSQGMKGAIAKAQDLAAATPGSFIPSQFTNPTNPRAHYETT
                     GVEIWEDTDGEVDIVVAGVGTGGTITGIGRLLKERKPGGITVIAVEPADSPVLSAGQA
                     GSHQIQGIGAGFIPETLDTDIYDEVITVSNEDAFSTAKELGVREGILTGISSGAALWA
                     AVQVAKRKENTGKNVVVILPDSADRYYSTPLFI"
BASE COUNT         1852 a         2010 c         2302 g         2304 t
ORIGIN      
        1 atccgataat atccgcaagg cgggggaaag aaccgggatt gccgtaagca tagcccattc
       61 ctccgccggc caggatgtca tatcccagga gtttcccttc tttttcaata gccacgaagc
      121 tcaggtcctg ggcgtacaca tccacatcat tttccggggg aagcgtaaaa gcgattttaa
      181 atttgcgcgg caaatagccg ggaccgtaca aaggctcttc ttccgcgccc gagtagattc
      241 gtttggcatc cagccatatt tcatgatagg cgtgcgtctg cggttccagt ttccgggaga
      301 gttcccgggc ctgattgaag acagtttcat ggatgccgga atcttccggg tgcagagaaa
      361 ccatgacatt gcgcgtggcg tccccactgg cggcaatcgt ggtgatggcc gcgtggtgga
      421 tatctttcat ggtctgtttc aagtcctgtt tgacgactcc gtggatttga atggcctggc
      481 gtgtcgtaag tttaagggtg ccgttgccga attggtcgct tagcgtttcc gcggccagcc
      541 attggctggg cgtcatgcgg cccccgggga ggcgcagcct caacatgaat aaccagtttc
      601 tttcctgtcc gtttttcttc cggataagcc gttcatcccg gttatcctgc tgggtaatcc
      661 cgtgaaattt caaaagaaat tcttcttcgg acgggaaatg ggtaatgctg ccgtcatgga
      721 ggaggtcaga gatgtttcct ctgaggaaat cgctttcctc cttgattcgt tcgttggatg
      781 tgtatccttc tttagtcatg atattttctg ttggttaagc taaattagtc ccccagcgtc
      841 tgtagatggc ttgttccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnagag caggcctatg
      901 agaataataa cgaacatgat gcccatgacc tgctccatgg aattgagttc tcttccgaag
      961 tgcaggagct gtcccaatcc cagtcctgtc aggatgggaa caaaaatttc tgcggacatc
     1021 agggagcgcc aggaaaaggc ccagccctgc ttcatgccgc tgacgatgaa tggagcagag
     1081 gccggaagcg tgacgaagag cagcgtgtag gcagagccgg ctcccattgt ccgtgctgcg
     1141 cgggcataaa tcggagggac gttggcaagt ccattttctg tggccagtat gaccgaccag
     1201 acggtgccca tgacaacgac gaacaggatg gccgcttctt cctggccgaa ccacaggata
     1261 gccagaggga cccagcaaac gctgggaagc ccttgaaatc ccaatgccag aactccgatg
     1321 gtgtttctta ttagctggaa acgggcagat aaaagtcctg ccggaattcc gatggagatt
     1381 ccggccaggt accccaggag cagtctccgg atagtaacga ccgtagcgga ccagagagaa
     1441 ttgtccctga ttgcttcttc aagatagccg cttatggcgg aaggcgccgg gaataggaaa
     1501 ggcctccacc aaccggatag agaaccgtat tcccatatag ccaaggccag caagatgaat
     1561 actgaggatt ggagaatatt ttttatggaa gggtgcatga tgtttgtgcg tcttgttttt
     1621 cttctgcggg cctggaaatc gattttaatg cccgtgtgat gtttgaagct agccgggcca
     1681 ctcccggatc attgatttgg cgcgggcgcg gcaaacgaat gcagaattct ccgcaaatac
     1741 gtccagggtg ggatgaaaat aaaatgactc tgtctcccag gcatacggct tcccgtacat
     1801 tatgagtgac aaagacgacg gtaattccat gcccggcgca aatggcctgg aggtccgcat
     1861 aaagctgttc cctggtcatg gcatccaatg cggcgaaggg ctcatccatc aggagaaccc
     1921 tgggacgcaa tgcaagggcc cgggctatgg ccacgcgctg tttcattcct ccggagagtt
     1981 catggatacg agcatgcgca aagttgtcca agcctacaga tttcaggttt tccaaagcta
     2041 tttggaggcg ttttgcgcgg ggaatttcgg taaatcggag accgaattct acgttgtgca
     2101 gcacgtttaa ccaggggaac agggcgaaat cctgaaaaac gactgtcctg tccggtcccg
     2161 gccctgagat tatttcccca tcataggaga tgcttcccgt atccgatttg atcagaccgg
     2221 cgatcatatt cagcagagtt gttttgccgc aaccgcttgg acctaccagg cagatgaatt
     2281 ctccttcccc tatgtccaga ttggcggaag aaagagccag aagcgatttt tttctggaaa
     2341 aataactttt ggaaacgttt cgtatggata atctactcat gggtgggatt atttttatca
     2401 ttccggatga tgccttcgac gtgggggagt ttcttgtaaa atccggtccg taccgtatcc
     2461 ttggctaatt cgtggagctt ttcttcggat attttattcg tgaaaactaa cctgggccat
     2521 gcatggagaa ttaaagaggg atcaatgctg gaacgggtga gttccctcaa ttcttctacg
     2581 acgatttttt gagcttcctg gggatgtccg atgatccaga gagtgagttc tctgtgagct
     2641 tcaatcatgg cctgcgccac gtccggctgt ttttccagga agtccttttg ggctgtaaga
     2701 acagtggtta cggcatcctt ttcaagaaat aggagcttgc ctccggcttc tttttccagg
     2761 cggctaatcc atggttctac agtccaggag ccgtccagtt tgccttgacg caagaggggt
     2821 atctgttctg gggtgggggt aggaagtatg cttgcgtctc ctccggtttg agtgactgtt
     2881 acgcctccca gggccaacca tgtccggcag gctatatcct gagtattgcc caattggggg
     2941 gtagcgatga ttttcccttg aaagtctttt tgcgtgcatg ctccggaatg ggagggaacg
     3001 accagtccgg accctccctg tacggctccc gctacgattt gcaggagagt tccgttggat
     3061 ttggaatacg cattaatggc aggggccggc ccaacgtagg taaaatccag ggaattggcg
     3121 aacatggctt ccatggcact ggggccggcg ttgtaagcat accatagaat ggagatgttt
     3181 ttccccgtcc gttcaaagat gcgtttttca aaccatccct cccccgttcg gctgaaatgc
     3241 ctggcaacca acccctggac atgggttacg ttggggaaaa ggccaaagcg tatttgatcc
     3301 tgccgttcgg atgcttcccg acaggatgcc agaaaggcag gggacaggag ggcgccaacg
     3361 cagagcagcg tgtttactgc aagaggcaaa atcttcatga aatgagggag caggtgtttg
     3421 gatgggataa aattaaaatt ccacatagag agtgcgcgcc agaagagacg cagtattttc
     3481 attcgcttct gcggggggaa ggatgcggtc tgccgtttct ggcgcggaga cgacattccc
     3541 aagccagatg cagtaggctc cctttgcttt tctgggcagg acgggaaggc ccggcagcgc
     3601 tgcaatgctc atgagccccg cttcggccag caggcctgag gcattgacga tttctctggc
     3661 ggatctgccc catacgcctt ccgccttgaa atccagaccg taggtatgag ccttgagccg
     3721 gtggagttcc cgttccagct gtttggcgaa gctgcgtgct aggtcgctgt cttctcccgc
     3781 tacgtaaatt tgttttccgc catgcccgaa agtttccatg cgttcatcgc ttgagacgaa
     3841 tgtcgggagt tgttcctgtt ctttttcagg aaccttatct atgtgagagg gacggagcat
     3901 tcctgctccc gccgtgacgt tattcagcgg gtcaatcagg atgagagctc ctccggagcg
     3961 gttatcccgg taataatcat agaacagggg agaagcggtt tccagaacga tgcggccgac
     4021 atggttcaga ggaagctgcg ttgcatggcg gctttccagt gtattgacgt ctatgtcgta
     4081 ctggagttct gctatccgcc cctggatatt tctgcccgca tggcgtataa tgtatttgcc
     4141 tccgggaaga aggggttttt cagacatcca gatgacacgg gcttccagcc gatcctcgat
     4201 atgggggaga ttcccttttt tgacgatcat gtcaccgctg ctgatatcta tttcatcctc
     4261 aagttccaat acgacggact ggggagagaa tgcttcttcc aggtctccgt ccggagtaac
     4321 aatccttctg atccggctgt tttgaaacga aggcagcgtc acgatagggt ccccccggcg
     4381 gatggagccg gaagtaatgg agccggcgaa cccccggaaa tcgagattgg ggcgtatgac
     4441 tgtctgtacc ggaaagcgga aatcccggag attcctgctg tcgctggcat ccagcgtttc
     4501 caggatggaa agcagatgat cgccttggta ccagggcgtt tttcctgttg tttgcgtcac
     4561 attttcccct tcaatggcgg aaatgggaac gaaacgaaca tccgggatat tcaactgttg
     4621 cgtgaagctt ccgaattctt cttcaatctt ccggaatttt tcttcagagt aattcagaag
     4681 atccatcttg ttgacggcta cgatgaggtg ccgtattttc agaagggagg cgatgaacgc
     4741 atgccgtttc gtctgtgtga gtactccgtg gcgagcatca atcagaatga tggccgcgtc
     4801 cgccgtggaa gctccggtgg ccatgtttcg ggtgtattgt tcatgtccgg ggcagtcggc
     4861 aatgatgaat ttgcggcggg gggtggtgaa gtaccgatag gctacatcaa tggttattcc
     4921 ctgttcccgt tccgctttaa ggccgtccag caacagggcg taatcaattt tacctgcccc
     4981 agcggttcca tttttttcac tggctttgcg cagctctgcc agctggtcgt caaaaatcag
     5041 tttgctgtca taaaggaggc gtccgatgag tgtagatttc ccgtcgtcca cggaaccgca
     5101 ggtaagtacg cgcagcaggc ttttattttc gtgttcgttc aggtatgagt cgatgtccat
     5161 aatggtaaaa ataagaggtg tcgtcagaat ggttcataat aggggttaaa aatatccttc
     5221 tcgttttttc tgttccatgg aggcatctcc gtcgtggtca atgatacggg aagaacgctc
     5281 gctcagcctg gtttccagca tttccgccac aatctcttct actgtgcggg ctgtagattc
     5341 tatggctccg gttagagggt aacagcccag tgtgcggaaa cggacggttc tcatttgcgg
     5401 tgtttccccc ggcttcaaag gcaggcggtt gtcgtccgcc ataatcaggg agccgtcacg
     5461 ttccacaatg ggacggggtt tggaaaaata caggtctaca acctgtattt tttccaggcg
     5521 gatgtattgc cagatatcca gttccgtcca gttggacaga ggaaagatac ggacgctttc
     5581 tcccggattg atacgaccgt tataaatgtt ccataattcc ggccgttgat tttttgggtc
     5641 ccattggttg aatttatccc ggaaagagaa gattcgttcc ttggcccgtg atttttcttc
     5701 gtcgcggcgg gcgcctccga atacggcatc atattttcct gcggtcaacg cctgaatcaa
     5761 tgcctgtgtt ttcataatat ccgtgtattt gcggctgccg taagtaaaag gattaacgcc
     5821 ggcggcaatg ccctcctggt tactgtggac aatcagatcc agatcatttt cctgaacgaa
     5881 tctgtcccgg aattctatca tctcgcgaaa tttgaatgtg gagtctatat gaagaagctt
     5941 gaatgggagt ctgcccggat agaatgcctt tttggctaag tggaccagta cagaggaatc
     6001 tttcccgatg gaataaagaa gtacgggatt ttcaaactgg ctgatggcat cccggaaaat
     6061 gtgaatgctt tctgcctcca actgctttag ctggctgagt ctgtaaatgc tgttcatttt
     6121 atatgttttt ggttatcaat gtcttggttt gcgatgtaaa ccgcactccc tgtgttcttt
     6181 tttttcccac caccatcgtc cgtcgcgtac atcttccgtc ctcttgacag atcttgtgca
     6241 gcaggcgcac ccgatactgg ggaatcctgc atcgtgaaga ggattgtagg gaacgccgta
     6301 ggcgcggata tagttccaga catcttcttc tgaccagttg gccagtgggt tgattttgat
     6361 gatgccgttc gcctcatcgt tttctgtgat atgaagccct ttccgggtga tggactgagt
     6421 ggagcgtaag ccgcagatcc acgctttttt cccggcaagt agtctttcca gagggcgcag
     6481 tttacggata ttgcagcatt cgtgacggta ttccatactg ttgcggaaca gattgacgcc
     6541 tttgtccaga atcatgtttt ccacttcttc cgctaccgga aaataaggaa taatgcgaat
     6601 gccgtatatt tcctccgttc ttgcccagag ttcataggtt tccggaaaca agcgccccgt
     6661 gtccagagtc gccagaggaa tattcagacc aagatgggag atcatatggg tgagcacttg
     6721 gtcttctgcc ccgaatgatg tagcaaaaac aaggtttcct tcgtattgcg ctgcggcttg
     6781 tcgcaacgtg tctgtagcgg ttggggaatg agcgttcatg aatgttggag ctggttggag
     6841 gtggatagcc gtatccggta gaaaagaacg ggatgtcaaa tgaacagagg agttgagtag
     6901 taccgatccg ctgaatcggg gaggatcact actacatttt tccctgtgtt ctcttttctt
     6961 ttagcaacct ggacggccgc ccatagggca gctccgctgg aaatcccagt cagaatgcct
     7021 tctctgacac ccaattcttt agctgtggaa aaggcatctt cgttggaaac tgtgatgact
     7081 tcatcataga tatccgtatc cagggtttct gggataaagc cggctcctat tccctgaatt
     7141 tgatggcttc cggcctggcc ggcggataat acgggggaat ctgccggttc cacggcaatg
     7201 acggtgattc ctccgggttt gcgttccttg agcagtctgc ctatgccggt tattgttccc
     7261 ccggtgccta ctccggctac gacgatatct acttctccgt ccgtatcttc ccatatttca
     7321 acacctgttg tttcataatg ggctctggga ttggtcgggt tggtgaattg actgggaatg
     7381 aaggagcccg gagtggcggc cgccagatcc tgggctttgg cgatggctcc tttcatccct
     7441 tggctgtctt ctgtcaggac aatatcggct ccgtaggctt ttagaatatt tcgtcgttca
     7501 acgctcatgc tttccggcat ggtaagaatg atgcggtatc cccgggaagc ggcaatagcc
     7561 gccaggccaa tgcccgtgtt gccgctggtt ggttcaatga tgacggctcc tggcttgagc
     7621 tggcccgatg cttctgcctc gttgatcatg gcgagtgcga tacggtcttt catgcttcct
     7681 gctggattca ggtgatccag cttgagcagg agccgggcat tcagacgatg tgcctgggca
     7741 taattccttg gctccagaag aggtgttttc ccgaccagtt ccgatatgga ggtgtaaatg
     7801 ttcatcgcgt cgtactttac agggatggat ttagatttct ttttattccg ataggaataa
     7861 tagggattat ttactcttat ccttcggaag tgtcaatgtg aattaatgaa tatgagatat
     7921 tattttttta tttgtttttt tatgtaaata aaaatggtag aagagtattg aatgaacagt
     7981 cgcaaaatgg cagaaactga gagcaaaagc ccggttcccc tctgttttca agctattgac
     8041 cgggagggag gatgaatggt gtgagggaca gaggtcttga tccctggcct tcctagagca
     8101 tgtaattgga taggctttct ccggttgacg ttatgaattt tcctttttcc ggagagtgat
     8161 tgcggcgttt ggaaaactgt ctgttttgtc caaatccatc ctttatcgca tatccttaga
     8221 atgtctgttt tctatatgct gataatctat cagttgtgat tatctggagg atgatgtgca
     8281 agaaatgcca ctgctgaaaa actgatccgt taagccttat ggaggaagct tttgacacac
     8341 gaaaatcgga tcatgtcgtt tatcatgacc tctctttgcc ctgcatggcg gtttcttttt
     8401 ccccccggct atgatgatcg ccccggagat tctttcggct caggcaacag ggaatcgccg
     8461 tgcgggcagg aattgtttta tttttctttt ccgtggc
//
LOCUS       DAOMSU010000076         8486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_607_length_8486_cov_3.172459, whole genome shotgun sequence.
ACCESSION   DAOMSU010000076 DAOMSU010000000
VERSION     DAOMSU010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_607_length_8486_cov_3.172459"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1176)
                     /locus_tag="PK077_04765"
     CDS             complement(115..1176)
                     /locus_tag="PK077_04765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HRN23643.1"
                     /translation="MSAREAADKWGISQRRVAVLCSESRIDNAIMVGNMWIIPTTAKK
                     PIDARSIRYVQSNGKKVKPFLKWAGGKGQLLSEIEKYYPFADGKITKYAEPFVGGGAV
                     LFDILSKYDLDEVYISDINAELANTYLIIRDNIDKLIAMLTVMQNEFVPTDTELRKAY
                     YLTKRERFNDLKVNGDETINIEKAALMIFLNKTCFNGLFRVNKKGLFNVPMGSYKNPM
                     ICDEDNLCAVSEKLQNVTIVCGDYRKSADFIDENTFVYFDPPYRPITDTASFTAYTEN
                     LFNDEEQIELARFIDDMHQRGAKMVISNSDPKNSNTEDDFFDNIYSSHRIKRVEATRM
                     INCNSKARGKIKELLISNF"
     gene            complement(1637..3160)
                     /gene="guaA"
                     /locus_tag="PK077_04770"
     CDS             complement(1637..3160)
                     /gene="guaA"
                     /locus_tag="PK077_04770"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRN23644.1"
                     /translation="MDDKHLVAVIDFGSQYTQLIVRRVRELGYMAKLYALEDLDQIHE
                     PGAVILSGGPKSTTDADAPDIDFEWLQSLNVPVLGVCYGMQLLNIKHGGTVKASNKRE
                     YGPAALLPETCAGLYRDMSPSSQVWMSHSDTVDHLAEGCRVIARNAEGVPVSLQWGET
                     TFGIQFHPEVTHSHEGRTILRNFLSCAANLKKFDIGDFKRELIREIRERVGNRQVVCG
                     VSGGVDSTVLAVLLHEAGVNMRAIFVDNGLLRKNEAEEVRANFARMNVEIETVDASER
                     FLAALDGESDPEKKRRIIGGLFIDVFWDAVGDAEMLAQGTLYPDVIESASNAKSKASV
                     IKTHHNRVERVLELQAQGKVLEPLAELFKDEVRELGASMGIPHDILWRHPFPGPGLAV
                     RCPGVVTKERLDIIRECDAIFIGNLKKYGWYDKVWQAYAGLIPVKTVGVKGDERSYEW
                     ATNLRAIVSEDAMTADWVELPPALLRETSNRILNEVKGINRVLYDISTKPPASIEWE"
     gene            complement(3173..4624)
                     /gene="guaB"
                     /locus_tag="PK077_04775"
     CDS             complement(3173..4624)
                     /gene="guaB"
                     /locus_tag="PK077_04775"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HRN23645.1"
                     /translation="MADFPLGLSFDDVLLLPRLSAILPGDADISSQLVPGFDMKIPVL
                     SAAMDTVSESELAIALAREGGLAVIHRNNPIDIQAAMVSRVKRFENAVIPNPVTVNKD
                     MTLEEVHQIMMDQGYSGFPVVDANRKLEGIITGRDMRGVDDYQNVRVEDVMTPLPRLI
                     TAAPTTTIEEARHILYTHRIEKLPLVDEHGVLAGLITETDIQKRAMFADASKDEHGHL
                     RCGAAVGVGPDYLDRAKALISAGADALFIDAATGHTTRVMDVVSNLRKLTDRPIVAGN
                     VVTAEGAADLIKAGVQAIKVGVGPGSICTTRVISGVGMPQFTAIQEVASVARPAGVTV
                     IADGGIRYSGDIVKALAAGADLVMLGGLLAGTEESPGKVVHYQGRHFKQYRGMGSLGA
                     MRRGSGDRYGQNSSGKLVAEGVEARVPYKGMLADVVFQLMGGLRSGMGYLGAHNLEEL
                     RNKARFVQITSGGLKESHPHDITITEEPVNYSC"
     gene            4813..5307
                     /locus_tag="PK077_04780"
     CDS             4813..5307
                     /locus_tag="PK077_04780"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HRN23646.1"
                     /translation="MSAPEPYRVLFVCLGNICRSPAAEIIFKKMVAEQGLEHLIESDS
                     AGMIGYHRGCPPDSRMLTALKKYGYEDPGLKSRPVRKEDLEQFDLIVGMDRENLRDLK
                     RLDKKGQWAGKIAPMCFFTTRFPDEEVPDPYYGGQEGFEYVVKLLQDGCGNLLERLKE
                     QLSL"
     gene            5761..>8486
                     /locus_tag="PK077_04785"
     CDS             5761..>8486
                     /locus_tag="PK077_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRN23647.1"
                     /translation="MKEQSFDNDVNSLTGMSNSNSGAPSAIDKQAPTDAFERSWDWDF
                     EVRELGPDETAECKVTMGADDLANLTVDGEERLDIGPRGQYGGGSYEPQTASFIIEPG
                     MHRAHVDYSNISIPNANNNIAKFSFDLKVEITNRKTGSSSSYVPPEAETEPVDNNDEG
                     DDDPCGGSSSGSSSSPNSSSSNPCPNGDNGGDEDETDPDNPFSPDDCMDNRGGSPSAS
                     AVRSLVSSASAYGKFSSAGKRVTAQTRKTSMVWRTSFGSFRGMEGVPYGMLEIVAYHF
                     SSRLWTPAALQYLHPMASCILPPSGRELGADMAFQIRNGGTCANYYCYAGAASAGSIG
                     GSRKRTGSVSMAYAAAEGRAVSASASAAEMRVSNAGGNTVIYGGSSVSALGAASGYRT
                     RLGSSWTAQDFANYLDIVRSADDVICQVWNLWDGLANIENVTDTGYVIAFYLPEQVGA
                     KNVSTGLYAVTGTPFKTFTIEGNAETGKLTVTEQAEGRAPYVTRYWQGTGGAWCMSQG
                     EGEDAIYTIRERQEVSSGIWKLFTTVQRGENGAPISRVCETYEQGRSGNLCTSRIEAY
                     GTDYARETTYAYNAAGKLIRETAPDGSEKTWSYDAFGRETVRMEPWAGGGRKGTYTYY
                     RCSDHADPDIAHQYVVLTMNAARLTDTHYAYTEANHVRRVEKRTTALGAEGEQLEVTE
                     TWLPAAPNEYARGRLKMKQSASGVQTVYGYEAASQYGALYRETRETQIAGQAVPGHST
                     RKVTYVSAQGNNTRVEKYALLTDGTWTLTDTADYEYDKENRWIKRTRGNGRVTEREMM
                     CCGPLWEKDEDGIMTTYSYNTARQLVEVSRSEVTDGETIVTPETIVSYTRDAFGRILQ
                     TRRDVGPMTTTESRAYDLLGQLVQETDVLGRSSTRAYSADGLTETVTTPTGAT"
BASE COUNT         1906 a         2198 c         2425 g         1957 t
ORIGIN      
        1 accttgaaaa acataaatga tataatcgtc aaagacaaat tctattattg cctttttggt
       61 cccattatct atacgttgtg ttccttctcg aattttcata gtcgtccccc cactttaaaa
      121 attgctaata agcaattcct taattttccc tcttgctttg ctgttacagt taatcatacg
      181 agtcgcttca acacgcttaa ttcgatgaga ggaatagata ttgtcaaaga aatcatcttc
      241 tgtattggag ttcttgggat cagaattgct gataaccatc tttgcacctc tctgatgcat
      301 atcatcaata aatctcgcaa gctctatctg ttcttcatcg ttaaacaagt tttcagtata
      361 tgcggtaaaa ctcgcagtat cggtgatagg acgataagga gggtcgaaat aaacaaaggt
      421 gttttcatca atgaagtctg ctgactttct ataatcaccg catacaatgg ttacgttctg
      481 aagtttctct gataccgcac aaagattatc ttcatcacaa atcatcggat ttttataaga
      541 gcccattgga acattgaaaa gtcctttttt attcacacgg aacagaccgt tgaagcaggt
      601 tttattaagg aaaatcatca atgctgcttt ttcgatattg atggtttcgt ctccattgac
      661 cttcaaatca ttaaaacgct cacgctttgt gaggtagtat gcttttctga gctcggtgtc
      721 cgtcggaaca aattcattct gcatcactgt gagcatcgca atcaatttat caatattgtc
      781 tcgaataatg agataggtat tggcaagttc ggcgttgatg tcactgatat aaacttcatc
      841 caagtcgtat ttgcttaaaa tatcgaacaa gacggcaccg ccaccgacaa atggttcggc
      901 gtattttgtg atttttccat ctgcaaaggg ataatatttt tcaatctctg aaaggagctg
      961 acctttgccg cctgcccatt tcaaaaaagg tttcactttt ttgccattac tctgcacata
     1021 gcggatactt ctcgcatcaa tcggcttttt ggcagtagtc ggtataatcc acatattgcc
     1081 gaccattatg gcattgtcta ttcggctctc agagcaaaga accgcaactc gtctttggga
     1141 aatcccccat ttatcagcag cttctcttgc ggacataaat tccataattg cacctctatg
     1201 tgtaactaat aatattatat tctaaaactc gaataatagc aataccattt cggaagataa
     1261 tttgtatctt ttttgtgacg atgggtaacg cccgcaggaa ctgtgatgct tcctcgagcg
     1321 gcatggtgat tagttattag ttgttgtgtt aatccatcag ccgatgcttc ggcatacctg
     1381 tcagattcac caccgagaac aagctgggca tattcaatag gcttgttata aattagccaa
     1441 tcaagttctg gctgctaatt gctatttcgc aaatggatgt taatatcatg taagaacaaa
     1501 aaagagctaa ttgatttaat caaaatcagt tagctcttta attatgaatg atgaattctt
     1561 gttgccaaat cattggcaca acttcgcaaa aatcagactt ttacggctct acagccgaga
     1621 aagtgcctct gcgaaattat tcccactcaa tggaagccgg gggtttggtg gagatgtcgt
     1681 aaaggacgcg gttgatgccc tttacttcat tgaggatgcg gttgctggtt tccctcagca
     1741 gggccggggg gagttccacc cagtccgccg tcatggcgtc ttccgacacg atggcgcgta
     1801 ggttggtagc ccattcatag gagcgttcgt cccccttcac tcccaccgtt tttacgggga
     1861 tgaggccggc ataggcctgc cagactttgt cgtaccagcc gtattttttc agattgccga
     1921 tgaaaatggc atcgcattcc cggatgatgt ccaggcgttc tttggtgact acgccggggc
     1981 agcgcacggc caggccgggg ccggggaagg ggtggcgcca cagaatatca tgcgggatgc
     2041 ccatggaggc gcccagttcc cgcacctcgt ccttgaacag ttccgccaag ggttccagta
     2101 ctttcccctg cgcttggagt tccagcacgc gttccacgcg attgtggtgg gtcttgatga
     2161 cggaggcctt ggatttggcg ttggaggcgc tttcgatcac gtccgggtac agggtgccct
     2221 gagcgagcat ttccgcgtct cccacggcat cccagaaaac gtcgatgaac aggcctccga
     2281 taatgcggcg tttcttttcc ggatcgcttt ctccgtctag agccgccagg aagcgttcgg
     2341 aagcatccac cgtttcaatt tccacgttca tacgggcgaa attggcccgc acttcctctg
     2401 cctcgttttt gcggagcaga ccgttatcca caaagatggc gcgcatgttc acgcccgctt
     2461 catgcaacag aacggccaga acggtgctgt ccacgccgcc ggagacgccg cagaccacct
     2521 gcctgttgcc tacgcgttcc cggatttccc ggatgagttc ccttttaaag tctccgatgt
     2581 cgaacttttt aagattggcc gcacaggaaa ggaagttgcg caggatggtg cgcccttcat
     2641 gggaatgggt cacttccgga tgaaattgga tgccgaaggt ggtttctccc cattggaggg
     2701 agacggggac gccctccgca ttgcgggcga tgacgcggca gccttccgcc agatggtcca
     2761 ccgtgtccga atggctcatc catacctggg aggaggggga catgtcccgg tacaggcccg
     2821 cgcaggtttc cggcagcagg gcggcggggc cgtattcccg cttgttgctg gcttttacgg
     2881 tgccgccgtg cttgatgttc agcagctgca tgccgtagca cacgcccagg acggggacat
     2941 tcaggctttg gagccattca aagtcaatgt ccggagcgtc cgcatccgtg gtgcttttgg
     3001 ggccgccgga aaggatgacg gcgccgggtt cgtgaatctg gtccaggtct tccagcgcat
     3061 acagcttggc catgtagccc agttcgcgca cgcggcgtac gatgagctgg gtgtattggg
     3121 agccgaagtc aatgacggct acgaggtgct tgtcgtccat gtgagggagg agttaacagg
     3181 aataattgac gggttcttcc gtgatggtga tatcgtgggg atggctttcc ttcagcccgc
     3241 cggaggtgat ttgaacgaac cgggccttgt tccggagttc ctccaggttg tgcgcgccca
     3301 gatagcccat gccggagcgc aggccgccca tgagctggaa taccacgtcc gccagcatgc
     3361 ccttgtacgg aacgcgcgct tccacgcctt ccgcgacgag cttgccggag ctgttctgac
     3421 cgtagcggtc gccggagccg cggcgcatgg ctcccaggga gcccatgccg cgatactgct
     3481 tgaagtggcg gccctggtaa tgaaccacct tgccggggct ttcttccgta cctgccagca
     3541 gtccgcccag catcaccagg tcggcgccgg cggccagggc tttcacaatg tccccggaat
     3601 agcggatgcc gccgtccgca atgacggtga cgcccgcggg acgcgcaacg gaggctacct
     3661 cctgaatggc ggtgaattgg ggcatgccca cgccggagat gacgcgggtg gtgcaaatgg
     3721 agccggggcc cacgcccacc ttgatggcct gcacgcccgc cttgataagg tcggccgctc
     3781 cttccgccgt aaccacgttg ccggctacga tgggacggtc cgtcagcttg cggaggttgg
     3841 aaaccacgtc catcactcgg gtcgtgtgtc ccgtggcggc gtcaatgaac agggcgtccg
     3901 ctccggcgga aatcagggct ttggcgcggt ccagataatc agggcccaca cccacggcgg
     3961 cgccgcagcg caggtggccg tgttcgtcct tggaagcgtc tgcaaacatg gcgcgcttct
     4021 ggatgtccgt ttccgtgatg aggccggcca gcacaccgtg ttcatccacc aggggaagct
     4081 tttcaatgcg gtgggtgtaa aggatgtggc gcgcttcctc aatggtcgtg gtgggagccg
     4141 ccgtaatcag gcggggaagg ggagtcatga cgtcctcgac tcggacattt tggtaatcgt
     4201 ccacgccgcg catgtcgcgg ccggtgatga tgccttccag cttgcggttg gcatccacga
     4261 cggggaagcc ggaatagcct tggtccatca tgatctggtg gacttcttcc agcgtcatgt
     4321 ccttgttgac cgtgacgggg ttcgggatga ccgcgttttc aaaacgcttc acgcgggaaa
     4381 ccatggccgc ctggatgtca atggggttgt tgcggtggat gacggccaga ccgccttccc
     4441 gcgccagggc aatcgccagt tccgattcgg aaacggtatc catggccgcg gacaacacgg
     4501 ggattttcat gtcaaagccg ggaacaagct gggaactgat gtccgcgtcg ccgggaagaa
     4561 tcgcgctcag gcggggcagc aggagcacgt catcaaaact taatccaaga gggaaatctg
     4621 ccatgcgata atctacagcc ggacatcctt taacgcaagc cgaaacacag gagaatctta
     4681 tttttgaatg agcggcgcag tttctttctc tgccccatgc catattcgcg ggcgtttccg
     4741 tttcccgtgc agaaactctt ccctcttgaa atggggaggc cgcctgccta tcctgaaaaa
     4801 gaaatgacgt ccatgagtgc tcccgaacct taccgagtcc tgtttgtctg tctgggcaac
     4861 atctgccgct ctcctgccgc ggaaatcatt ttcaaaaaaa tggtcgcaga acaaggtctg
     4921 gaacatttga ttgagagcga ttccgccgga atgatcggtt accatcgggg ctgccctccg
     4981 gatagccgga tgctgacggc gctgaaaaaa tacggatatg aggatccggg gcttaagtcg
     5041 cgccccgtgc ggaaagagga tttggaacaa tttgacctga ttgtgggaat ggaccgtgaa
     5101 aatctgcggg atttgaaacg gttggataaa aagggacagt gggcaggcaa gatagcgccc
     5161 atgtgttttt tcacgactcg ttttccggat gaagaagtgc cggatcccta ttatgggggg
     5221 caggaaggct ttgaatatgt ggtaaaactg cttcaggacg gttgcggcaa tctgctggag
     5281 cgtttgaagg agcagctttc cttgtaaatt caggcgtgga ggctgggaaa gtggtggttt
     5341 atcctgcttt ctctccattc agacatgccg gggttgctga acatgcccgc aggcgataac
     5401 cgtttttatg gattttggat ttcttttcca attcagggag atcatgcttt ttccgttgaa
     5461 gagttcatga atccatgtta aatgaaaaat tgctgtgttt catggcgcgc agttaaaggc
     5521 catttggata tgttgcttcc ggaattttcc atatggacca gagttttcca ttcggaagtg
     5581 cagagaagaa atttttacca tttattgaac cacggctgag gccagggggc atcccgtgtt
     5641 ggacgtgttg aaacaggagg ttttgttgtt atggataagg aacggataaa aattattgca
     5701 gtttttccaa gattgggatg atgcaatgtt tgcgtttcaa tagctaatcc gattcaaacc
     5761 atgaaagaac aatcctttga taacgatgtc aattccctga cgggcatgag caattccaat
     5821 agcggagctc cctccgcgat cgacaaacaa gcgccaacgg acgcttttga aagaagctgg
     5881 gactgggatt ttgaagtcag ggagctgggg cctgatgaaa cggccgaatg caaggtgacc
     5941 atgggcgccg acgatctggc taacttgacc gtggatggag aagaacggct ggacatcggc
     6001 ccgcgcggac agtacggagg cggcagttac gagccgcaga cggcttcttt catcattgag
     6061 cccggaatgc atcgggcgca tgtggactat agcaatattt ccattcccaa tgccaataac
     6121 aacattgcca aattctcctt tgacctgaag gtggaaatta ccaaccggaa aacgggttct
     6181 tcttcttcct atgtgccccc ggaggcggaa acggagccag tggacaacaa cgacgagggc
     6241 gacgatgatc catgcggagg ctccagcagc ggaagcagca gttctcccaa cagcagttcc
     6301 agcaatccat gccctaatgg cgacaacggc ggggatgagg atgagactga tccggacaat
     6361 cctttttccc cggatgactg catggacaac cggggcggtt cccccagcgc gtctgccgtg
     6421 cgcagcctgg tttcctccgc ctccgcatat ggaaagttct cttccgcagg caaacgggta
     6481 actgcccaga cgcggaaaac cagcatggta tggcgcacca gcttcggttc cttccgcggt
     6541 atggaaggcg tgccgtacgg gatgctggaa attgtggctt atcacttctc ttccaggttg
     6601 tggacgcccg cagccctgca atacttgcat cccatggcca gctgtattct gcccccttcc
     6661 ggtcgggagt tgggggctga catggcattc cagatccgga acggaggcac ctgcgccaac
     6721 tattactgct atgccggcgc ggccagtgca ggctccatcg gcggctcgcg gaaaaggacg
     6781 ggttccgttt ccatggcgta tgccgcggca gaggggcgtg ctgtctcggc ttccgccagc
     6841 gcggcggaaa tgagggtcag caacgccggg ggcaatacag tcatctacgg cggttcttcc
     6901 gtttccgctt tgggtgcggc ctccggctac cggaccaggc tgggttcttc atggacggct
     6961 caggattttg ccaattacct ggacatcgtc cgaagcgcag atgatgtcat ctgccaggtc
     7021 tggaacctgt gggacggtct ggccaatatt gagaacgtga cggatacggg ctatgtgatc
     7081 gccttttatc tgcccgagca ggtgggggcc aaaaacgttt ccaccggtct ttacgccgtt
     7141 acggggacgc cttttaaaac cttcaccatt gagggcaatg cggagaccgg caagctcacc
     7201 gtcacggagc aggcggaagg gcgcgcgcct tacgtcaccc gctactggca gggaacggga
     7261 ggggcctggt gcatgtccca gggggaaggg gaagacgcga tttacacgat ccgtgaaagg
     7321 caggaggtgt cttcggggat ttggaaactc ttcactaccg tgcagcgcgg ggaaaacggc
     7381 gctcctattt cccgggtgtg cgaaacctat gaacagggcc gcagcggcaa cctgtgcacc
     7441 agccgtattg aggcttatgg aaccgactat gcccgtgaaa cgacttacgc ttataacgcc
     7501 gcgggcaaac tgatccggga gacggccccc gacggaagtg aaaagacctg gtcttacgac
     7561 gccttcgggc gtgaaaccgt ccggatggaa ccctgggcgg gcgggggaag gaagggcacc
     7621 tacacctatt accgctgctc cgaccatgcc gatccggata ttgcgcacca gtacgtggtg
     7681 ctcactatga acgctgcccg gctgacggat acgcattacg cctatacgga agccaaccat
     7741 gtgcgccgtg tggaaaaacg caccacggcg ttgggcgcgg agggagagca gctggaagtg
     7801 acggaaacgt ggctgccggc ggcgcctaac gaatacgccc gggggaggct gaagatgaag
     7861 cagtccgcca gcggcgtgca gacggtctat ggctatgagg cggccagcca gtacggcgcc
     7921 ctctacaggg agaccaggga aacgcagata gcggggcagg ccgtgccggg gcacagcacg
     7981 aggaaagtca cgtacgtttc cgctcaggga aataacacgc gcgttgagaa atacgccttg
     8041 ctgacggatg gaacctggac gctgacggat acggcggatt acgaatacga caaggaaaac
     8101 cgatggatta agcgtacgcg cggcaacggc agggtgacgg aacgggagat gatgtgctgc
     8161 ggccccttgt gggaaaagga tgaagacggc atcatgacta cttactccta caatacggcg
     8221 cgccagctgg tggaagtcag ccggtcggaa gtcacggacg gagaaacaat cgtcacgcct
     8281 gaaaccatcg tcagctacac gcgggacgcc ttcggcagaa tcctgcaaac gcgccgggac
     8341 gtcggcccca tgacgacgac ggaaagcagg gcctacgatt tgctggggca actggtgcag
     8401 gaaacggacg tattaggaag gagcagtacg cgtgcctaca gtgcggacgg cctgacggaa
     8461 accgtcacga cgccgacggg cgcgac
//
LOCUS       DAOMSU010000077         8392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_623_length_8392_cov_2.550558, whole genome shotgun sequence.
ACCESSION   DAOMSU010000077 DAOMSU010000000
VERSION     DAOMSU010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8392
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_623_length_8392_cov_2.550558"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..1195)
                     /locus_tag="PK077_04790"
     CDS             complement(233..1195)
                     /locus_tag="PK077_04790"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HRN23648.1"
                     /translation="MYDVVALGELLIDFTPCGINDRELPVYQANPGGAPCNVLSMLSR
                     LGRKTSFIGKVGHDMFGKMLRRTLQEEGIGDSGLVASREVNTTLAFVQIDEHGDREFS
                     FYRNPGADMKLTAGEVDLELVEHARVFHFGTISMTHDDVRRATRHAVSHARKKGELIS
                     FDPNLRPPLWPDMKLAREQMLYGCGACGIMKIEMEELFFLTGCATMEEGLRILQREFD
                     NLRLILVTGGRKGSWAAYESKLIHQPTYLNVKTIDTTGAGDAFLGCCLDRILETGLEN
                     LTEGQLADMLLFANAAASIVTTRKGAIRSMPSREEAVALMAEGF"
     gene            1608..2111
                     /locus_tag="PK077_04795"
     CDS             1608..2111
                     /locus_tag="PK077_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HRN23649.1"
                     /translation="MQEKSSGFQWPAQWTPDILATLMFVVQKGRILLIRKKRGIGAGK
                     VNGPGGKFEPGETALQCVLREVREELHIDIEDAREMGVLNFSFACGTIPEIRCHVFMA
                     TSFTGTPTETPEAEPFWCPVDGIPYDLMWQDDRFWLPAMLDGKRFEAFFTFEGDRMME
                     FSLKTGD"
     gene            2960..4156
                     /gene="kbl"
                     /locus_tag="PK077_04800"
     CDS             2960..4156
                     /gene="kbl"
                     /locus_tag="PK077_04800"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRN23650.1"
                     /translation="MYTPEFKNILTSTLDGLRAEGLYKEERFIASQQYSQVTLKDGRS
                     INMCANNYLGLANNPEVMEAAKKAIDQWGFGMASVRFICGTQTLHRQLEERLTQFLGT
                     EDTILFPSCFDANGGLFEGLLTADDAIISDSLNHASIIDGVRLCKAKRFRYANNDMAD
                     LEAKLQEADAAGARVKLISTDGVFSMDGIIAQLDKIHELAAKYNAIVHFDDCHATGFL
                     GEKGRGTHEYRGLFGHIDITTGTLGKALGGASGGYVSGPKEVVDVLRQKARPYLFSNS
                     VAPAIVAASIKVLDLLEQSTEARDRVEANTKYFRDAMTGAGFTIGGKDHPISPVMLGD
                     AVLSQKFSAQLLDEGVYAVGFFYPVVPKGQARIRTQISAAHTREQLDKAIEAFCKVGK
                     NLGVIS"
     gene            4171..6810
                     /locus_tag="PK077_04805"
     CDS             4171..6810
                     /locus_tag="PK077_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRN23651.1"
                     /translation="MASDASPMSGGNLPTLPVKRVVRPTPSIPDHEVVRQIGSGAYGE
                     VWLAKSLTGAWRAVKIVWREDFEDERSFNREFEGILQYEPIARNHPGLVHILHVGRHD
                     QDSPFYYYVMELGDDARTGVHITPDEYIPRTLQTDKKFSGNKPLPLDYCLEVGSQLAH
                     ALLYLHSKNLTHRDIKPANVIFVNGRPKLADIGLVAHLDQRSFVGTEGFIPPDGPGTR
                     RADVYALAKVLYEISTGKDRMDFPELPDDLPEGTVRKKWQAFNTIICQAAEPRIEECP
                     IDSAEELAEKIDALRGYEVPSRFRLQKKKKRRKLRRALQLLAAAAAGALTAYWGALWL
                     NNSQHPPEEPASDIHPDIHSSAPEEESRTGYVLVTSSPAGASVYDADGNYLDETPYGP
                     IELPSGSPVAYTIKKSGFADKEEAGTVKGGATLALGGVLKEYHPPTDSQPWKDTEGVT
                     YLPAETRHVAQGPLTAALFNKFLKEDRQKGNFQMKREQTEPEHPEKDVALLTQDGITA
                     YLAWLNKKCEREGLLGKEFSINADPVPQASGSTENHNAYVLNVTRVFQVPITVTTNPP
                     GASVFFNNRLIGRTPIEEYVNQVPYVIEIKLPGHATMRRRGLDPQDLYLSLQLEPDKS
                     VVFGSPWTNSLGIKLVPVGGNALVMTHEVRVKDFQEFLKATGRKAPARPGFPQGEDHP
                     VVNVSRQDARAFARWLTSKERALGLIDQHDSYRLPKDEEWSSWVRLTDEQGASPYEKT
                     LPHENSREAFPWGYSWPPPDKTGNFADQSALIYLPSSRVIVGYNDGQPYTAPVKTFPP
                     NHLGLYDLEGNVMEWVDDSYGGPESLPIRNYGVARGGSYLSFRPKQLTTSIRTPLPEN
                     TRDNALGFRLILSSERPAIPTAP"
     gene            6973..7743
                     /locus_tag="PK077_04810"
     CDS             6973..7743
                     /locus_tag="PK077_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRN23652.1"
                     /translation="MKIIAIANQKGGVGKTTTAINMAAALAQLKKKILVIDLDPQANA
                     TSGMGIDGKDMPSLYPALLGQLPAEECILPSGRKRLDIIPSTMDLAGVEIELARSGNH
                     LTRLRETLAPLRESDTYDYCIMDTPPSLGVLMTSALAACDEVLTPLQCEWFGLEGLAK
                     ILHVTAQICASGANPRIRHEGVLMTMYDGRTNLSRQVIDQVERHLPQMLYKTVIPRSI
                     RLGEAPSFGHTIFEHDPSGAAADAYLKAAREFIKRHKP"
     assembly_gap    7767..7805
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7985..>8392
                     /locus_tag="PK077_04815"
     CDS             7985..>8392
                     /locus_tag="PK077_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23653.1"
                     /translation="MPAEEGQPDDQPRVTGQVPPPAPTTGLEQILPGRPPLPHAEEKG
                     AFFVYQRELAPGTRLEQYEIIRVLGSGGFGITYLAKDLFLNRNVVIKENFPSRYSYRD
                     PLTGHIRPNNEHDLENYTWALKSFLSEAQTLAEL"
BASE COUNT         1894 a         2500 c         2218 g         1741 t
ORIGIN      
        1 cgagtgccct cccgagcacc atcctctttc ataccgcaag ttgctggttt tcagctcttg
       61 cggttttcgt ttgccggcgg cgggaaaata agccgcaaca cattttttat caacagggga
      121 gggcatgcgc tataaaaaga aaggcatttc cccacttccg gcccccccga actcttactt
      181 gccggaaaca atccggacgt aatcagtaaa ggattggccc ttacggcatc cgttaaaaac
      241 cctccgccat cagggccacg gcttcttcac ggctcggcat ggaacggatg gctcccttgc
      301 gtgtcgtcac aatggaagcg gcggcattgg cgaataaaag catgtccgcc aactggcctt
      361 ccgtcaggtt ttccagcccg gtttccaaaa tccggtccag gcagcatccc aaaaaggcgt
      421 ctccggctcc tgttgtgtca atagtcttga cattcaagta cgtaggctgg tgaataagtt
      481 tgctttcata ggcagcccag cttccttttc tgccccccgt caccagaata aggcgcaaat
      541 tgtcgaactc cctctgcaaa atcctcaatc cttcttccat ggtggcgcag ccggtgagaa
      601 agaaaagttc ctccatttct attttcatga tgccgcatgc gccgcatccg tagagcattt
      661 gttcccgggc cagtttcata tcaggccaca ggggcggacg gagattgggg tcaaaagaga
      721 tgagttctcc ttttttccgc gcatggctga cggcatgccg ggtagccctc cgaacatcat
      781 catgcgtcat ggaaatcgtc ccgaaatgga agacgcgcgc gtgctccacc aattcaaggt
      841 ccacttctcc cgcagtcagc ttcatatcgg cgccgggatt acggtaaaag gaaaattcac
      901 ggtctccgtg ttcgtcaatc tgaacgaaag ccaacgtcgt attgacttcc cgggatgcga
      961 cgagacctga atctccgatg ccttcttcct gaagcgtgcg gcgaagcatt ttcccgaaca
     1021 tgtcgtggcc caccttgcca ataaaggagg ttttgcgccc gagcctggac agcatggaca
     1081 ggacgttgca gggcgctccg cccggattgg cctgataaac gggcagttcc cggtcattta
     1141 ttccgcaggg agtgaagtct atcagcaatt ctccgagagc tacgacgtca tacatgcttt
     1201 tgcaaatgtt gaaagtaaac cttccggcga aatataaacc gggagagata aagaagcata
     1261 gaacgcgacg gccttgcggg aaaaggaaaa agagattcat tccagtcatg gcggaaaagg
     1321 aatttcccct tttatcccgc cctgcagaaa cctcgaaggt tgatggagca tccgaatgtg
     1381 aacaagtatg gcatatatcc ctaacgccct ccactcagga aatgccccct tacgggcatt
     1441 tatgggcgcg acttcttctg ctccctccca ttcctccgca acaaacacgc attttcaagg
     1501 catttcaagc ttctttaaga gaattcaacc cttttcaagc atgtgtgatg gtcacagtat
     1561 tccgcatttt tcttcatgat tcccttccca tcctgtaata tgcgcccatg caagaaaaaa
     1621 gcagcggctt tcaatggccc gcacagtgga cccccgatat tctcgccacg ttaatgttcg
     1681 tggtgcagaa aggaaggatt ctcctgatac gcaaaaagag gggaatcggc gcgggcaagg
     1741 tgaacggccc cggcggaaag tttgaaccgg gggaaacggc cctgcaatgc gttttgcggg
     1801 aagttcggga agagcttcac attgatattg aggatgcccg ggaaatgggc gtgctgaatt
     1861 tttcctttgc ctgcggcacc attccggaaa tccgctgcca tgtgtttatg gcgacgtctt
     1921 ttacaggcac ccccacggaa acccccgagg cggagccctt ctggtgtccg gtggacggga
     1981 ttccctatga cctcatgtgg caggatgacc ggttctggct gcccgccatg ctggacggga
     2041 aacggtttga ggcgtttttc acgttcgagg gggaccggat gatggaattt tccctgaaaa
     2101 ccggggattg aaccgccaag aaaccccagg ttgcggcctc ctccctttaa gccggatggg
     2161 gaacgctgaa ctttttttat tccgtccttc cccacggagg caacagattc agaaggaggg
     2221 ggaaacagta ctgcccggcg ctccagaaaa gattccctga aattcacctg aaggtggggg
     2281 gcaaagaggg ggcatttgcc cctgaagacc attctgaaca gcagctaacg ttgaagggcc
     2341 ggcttccgaa aaaaatggac ctttcccctt gtaccgggat acatcatatt catctccttc
     2401 cctccaggta tcctgaaacg gatcatagga attgctccgg acgccagcat cgcagagatt
     2461 tccccatggc gtccagaaaa aggaaagttc tccctgccgg aaccggtttc cggcttgagg
     2521 ttcatgcgaa gggctggaat tcagccaaat ccgcggcttc gtaagaaaac ggggggggat
     2581 ggttgcataa ccggaccggt aatggaggtt taagggagca aatcagctcc ggcctttatt
     2641 ttttccaagc gcatccgaat atccactggt gcatcaaatc ccttttccgt aaacgggcgt
     2701 gggcttttct ttttgcccgg tatccgggaa atgctgattt ttccgatgtt ttgccttctt
     2761 tttcaggtaa tgcgcggatg cctgcgccct tgccgctttt ttactttgca cggggaaagg
     2821 acggctcccg cctccgggca tcaagccgta ttctttaagc ttttcctccc gcttccgttg
     2881 tgataatgat gcgggagcaa ccgtgtgatc cttgatcgtt cacttgctga ctatttcaat
     2941 tcatattcca ttattcaaca tgtatacccc cgaattcaaa aacatcctca cttccaccct
     3001 ggacggactg cgtgcggaag gactttacaa ggaagaacgc tttatcgcct cccagcagta
     3061 ctcccaggtg acgctgaagg acggacgctc catcaacatg tgcgccaata attatctggg
     3121 ccttgctaat aatccggaag tgatggaagc cgccaagaaa gccatcgacc agtggggttt
     3181 cggcatggcg tccgtgcgtt ttatctgcgg cacgcagacc cttcaccgtc aattggaaga
     3241 acgtcttacc caatttctcg gcacggaaga cacgattctg tttccctcct gctttgacgc
     3301 caacggcggg ctgtttgaag gcctcctcac tgcagacgac gctattattt ccgattccct
     3361 gaaccacgct tccattattg acggcgtgcg cctctgcaaa gccaagcgct tccgttatgc
     3421 caacaacgac atggcagacc tggaagccaa gctccaggaa gcggacgccg ccggagcgcg
     3481 cgtgaagttg atttccacgg acggcgtctt ttccatggac ggcatcattg ctcagctgga
     3541 caagattcac gagctggcag ccaagtacaa tgccattgtc cactttgacg actgccacgc
     3601 cacgggcttc ctgggcgaaa agggccgcgg cacgcatgaa taccgtggac tgttcggcca
     3661 tatagacatc accaccggta ccctgggcaa ggcactggga ggcgcttccg gcggctacgt
     3721 ctccggcccg aaggaagtag tggatgtcct gcgccagaag gcgcgcccgt acctgttctc
     3781 caacagcgtg gccccggcta tcgtagccgc ttccatcaaa gtgctggacc tgttggaaca
     3841 gtccacggaa gcgcgcgacc gcgtagaagc caataccaag tatttccgtg acgccatgac
     3901 gggagccgga ttcaccatcg gcggcaagga ccaccccatt tccccggtca tgttggggga
     3961 tgccgtcctg tcccagaaat tctccgccca gcttttggac gaaggagtgt acgccgtcgg
     4021 cttcttctat cccgtcgttc ccaagggaca ggcccgcatc cgcacgcaga tttctgcggc
     4081 ccatacccgc gaacagctgg acaaggcgat tgaggccttc tgcaaggtag gtaaaaacct
     4141 gggcgttatt tcctgattcc cgcgctttcc atggcttcgg acgcatcacc catgtccggc
     4201 ggcaacctgc cgacgctgcc cgtcaagcgg gtggtgcgtc ccactccctc cattccggac
     4261 catgaagttg tgcgccagat agggagcggc gcgtacggag aagtatggct ggcaaaatcc
     4321 ctgaccgggg cgtggcgcgc cgtcaaaatc gtatggaggg aggattttga agacgagcgc
     4381 tctttcaacc gggaatttga gggcattctc cagtatgaac ccatcgcccg caaccatccg
     4441 gggctggtcc atatcctgca tgtagggagg catgaccagg attccccgtt ttattattac
     4501 gtcatggaac tgggcgacga cgcccgcacc ggagttcata ttacaccgga cgaatatatt
     4561 ccgcgcacgc tccagacgga caagaagttt tccggcaaca agcctctgcc cctggattac
     4621 tgcctggagg taggcagcca gctggcccac gccctgctgt acctgcacag caagaacctg
     4681 acgcaccggg acatcaagcc ggcgaacgtc atctttgtca acgggcgccc caaactggcg
     4741 gacataggcc tggtggccca tctggaccag cgcagctttg tagggacgga gggattcatc
     4801 ccccccgacg gccccggcac ccggcgggcg gacgtttacg cgctggccaa ggttctgtat
     4861 gaaatcagca cgggaaagga ccgcatggat tttcccgaac tcccggatga cttgccggaa
     4921 ggcaccgtgc gcaagaaatg gcaggccttc aacaccatca tttgccaggc ggcggaaccc
     4981 cgcattgaag aatgccccat tgattccgca gaggagctgg cggaaaaaat agacgccctc
     5041 cggggatatg aagtgccttc ccgtttccgt cttcagaaga aaaagaaacg ccgcaaactc
     5101 agacgggccc tccaacttct ggctgcagcc gccgccggag ccctgactgc ctactggggg
     5161 gctttatggc tcaacaacag ccagcatccg ccggaggaac ctgcctcgga tattcatccg
     5221 gatatccact catccgctcc ggaagaagaa tcccggaccg gatacgtgct ggtgaccagc
     5281 tccccggcgg gagcgtccgt gtatgatgcg gacggcaact atctggatga aactccctat
     5341 ggccccattg aactgccttc cggctccccg gtagcctata ccatcaagaa atcggggttt
     5401 gcggacaaag aagaggcggg aaccgtcaaa ggaggggcca cgctggccct gggcggcgta
     5461 ctgaaggagt accatccccc tacggacagc cagccctgga aggatacgga gggtgttacg
     5521 tacctgcccg cagaaacgcg ccatgtggcg caaggccccc ttaccgccgc cctgttcaac
     5581 aaatttctga aggaggaccg ccagaaaggg aacttccaga tgaaaaggga acagacggaa
     5641 ccggagcatc cggagaaaga tgtggccctg ctcacgcagg acggcatcac ggcctacctg
     5701 gcgtggctga ataagaaatg cgagcgggag gggctgctgg gcaaggaatt ttccatcaat
     5761 gcggatccgg ttccgcaggc ttccggcagc acggaaaacc acaatgccta cgtcctgaat
     5821 gtcacccgcg tgttccaggt gcccattacc gtcaccacta acccgccggg agccagcgtc
     5881 tttttcaata acaggctcat cggacgtacc cccattgaag aatacgtcaa ccaggttccc
     5941 tacgtcattg aaatcaaact gccggggcac gccaccatgc gtcgcagggg gctggatccg
     6001 caggacttat acctttccct gcagttggaa ccggacaagt ctgtcgtttt tggctcgccc
     6061 tggaccaaca gcctgggtat caaactggtc cctgtgggag gaaatgctct ggtcatgacc
     6121 cacgaagtgc gcgtaaagga ctttcaggaa ttcctgaaag ctaccggaag gaaagctccg
     6181 gcccggcccg gctttcccca gggagaagac cacccggtgg tgaatgtcag caggcaggat
     6241 gcgcgcgcct tcgccagatg gcttaccagc aaagagcggg ccctgggcct gattgatcag
     6301 cacgactcct accgtcttcc caaggatgag gaatggagtt cctgggtgcg tctgacggat
     6361 gaacagggag cctcccccta tgaaaagacg ctgcctcatg aaaactcccg ggaagccttc
     6421 ccgtgggggt attcatggcc gccgcctgat aaaacgggaa attttgcgga ccagtccgcc
     6481 ctgatttacc tgccgtcctc ccgcgttatc gtaggatata acgacggaca gccgtatacg
     6541 gcccccgtaa aaacctttcc gcccaaccac ctgggcctgt acgacctgga aggaaacgtc
     6601 atggaatggg tagacgattc ctacgggggg ccggaatccc tccccatccg caactacggc
     6661 gtcgcccgcg gaggcagcta cctgtccttc cgccccaaac agctcaccac ttccatccgc
     6721 actcccctgc cggaaaatac gagggataac gcgctgggct tccgtcttat cctgtcttcc
     6781 gaacgccccg ccattccaac agctccctga cgccgtatct gcggcagcgg ttttccccgc
     6841 tctttccccc tctgaaacac accgcccccc caagggggaa agccctgagg cttgagagag
     6901 cttccggctc tccgggaaaa tgattgaaag ggcctcccgt tatgctacca tttttccgca
     6961 tccttttcct gaatgaaaat catcgccatc gccaaccaga aggggggagt gggaaaaacc
     7021 actacggcca tcaacatggc tgccgccctg gctcagctga aaaagaaaat ccttgtcatt
     7081 gacctggacc cgcaggccaa tgccaccagc ggcatgggca ttgacggaaa agacatgccc
     7141 agcctgtatc cggccctgct cggccagctt ccggcggaag agtgcatcct gccttccggc
     7201 aggaaacggc tggatatcat cccctccacc atggacctgg ccggcgtgga aatagaattg
     7261 gcccgttccg gcaaccatct gacccgcctc cgggaaacgc tcgctcccct gcgggagtca
     7321 gacacctatg attactgcat catggacacg ccaccttccc tgggcgtttt aatgacctcc
     7381 gccctggccg cctgcgacga agtgctgact cccctccaat gcgaatggtt cggtttggaa
     7441 gggctggcca agattctgca cgtgacggcg caaatctgcg ccagcggcgc caatccccgc
     7501 attcggcatg aaggagtact gatgaccatg tatgacggcc gcaccaatct ttcccgccag
     7561 gtcatcgacc aggtggaacg gcatctgccc cagatgctgt acaaaacggt catcccccgt
     7621 tccatccggc tgggggaggc ccccagtttc ggccacacca tttttgaaca cgatccttcc
     7681 ggagctgcgg cggacgccta cctgaaagca gcccgggaat tcatcaaacg ccataaaccc
     7741 tgaacatttg aagaaacaag cttcccnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7801 nnnnnagggc accgcagaaa acttcctcct tcattttgcc ttccgggttt gttgcgtcca
     7861 tttaacgtca ggaaaacatc ttccataccg cccccccgga gacggaggaa aacaggcatt
     7921 ccccttgact tgacttccac ggcatataag aatgctccag ctccgtttca ggaactttcc
     7981 cctcatgcca gcagaagaag gccagccaga cgaccagccc cgggtgacag ggcaagtccc
     8041 gcctcccgct ccgacgacag gcctggaaca gattctgccc ggacgtccgc ccctgcccca
     8101 tgcggaagaa aaaggggcct ttttcgttta ccagcgggag ctggctcccg gcacgcgcct
     8161 ggaacaatat gaaatcatcc gcgtcctggg tagcggaggt tttggcatca catacctggc
     8221 gaaggatctg ttcctgaacc gcaacgtggt tatcaaggaa aacttccctt caagatactc
     8281 ctaccgggac cctctgaccg ggcatatccg gcccaataat gaacacgatc tggaaaatta
     8341 cacctgggct ctgaaaagct ttctgagcga ggctcagacg ctggcggagc tc
//
LOCUS       DAOMSU010000078         8350 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_629_length_8350_cov_2.587583, whole genome shotgun sequence.
ACCESSION   DAOMSU010000078 DAOMSU010000000
VERSION     DAOMSU010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8350
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_629_length_8350_cov_2.587583"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1342
                     /locus_tag="PK077_04820"
     CDS             <1..1342
                     /locus_tag="PK077_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23654.1"
                     /translation="GEKSFYDGNEDENGASAPKPAGADKPAPGLRGEKVLADGQPTQT
                     GLKGTFLFQARALKLSPQRVVIRGFTQDYKPGGKRVYSEPMIIFVLSKSEHAQMIRNE
                     LERITSDLEGMIRRMDAMADEAKRLKGMENGKLKDAESQERLHALADEEQANRRELGD
                     LLNRSESLFKEASRNSQIDPAGMKEFMKGISMLKPIPDGSMKMAQKLFRDSASESRSE
                     QESRKDLDDGENSHSEATQALKDAMNQLSKSAQDMEASTFVARLRQAASKEDSIAHAL
                     AGQINTIAGMTMEELDPSTRREMETIATLQNASTQDIGWILEDLSYYKSRTGERIYSD
                     LYNQMNAFSLREKLDLVHGNILNAITARSIDESRLYASTLRHWAKLIDDYKKSRRGGG
                     GGGGGGGGSQSSISDAEFEFMLKLIRMIQQEQDIRMRTRAAEQEHRKKLPPPAP"
     gene            1339..1992
                     /locus_tag="PK077_04825"
     CDS             1339..1992
                     /locus_tag="PK077_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23655.1"
                     /translation="MNTANFPAMILALSLPCAAAPAPAPEYSAKHRETASKLAVQQDE
                     LQADTSDLILGETNEEVVDLLKKCRHAMNDAVDLLEVYNTGGQTLAAQSDVIELIYQA
                     AKAKMTGADGRPKPGGRALMDMLRQLLGMDSDSQSAEQQEGNGKGQEGSQRGSGNNPG
                     RGANGKSNMASSQDKGVSNPDTAAETRSVPKSTGMSADDMPSEFRKALDAYNKTLQK"
     gene            1989..3047
                     /locus_tag="PK077_04830"
     CDS             1989..3047
                     /locus_tag="PK077_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRN23656.1"
                     /translation="MRRISPDSPFTHEQQLPVSMKNPRRLLTAFLTCCMTAAPQNATA
                     QSPMRTSSSVPPQVELMYVKGLRYLQNAQKTDGTYDGTYGREPGIIGFCLMSVLAHGD
                     DPNAGPYATMVRRCVDYILSRQNKVSGYIGDSMYNHGFATLALAEAYGMVRDDRIGPA
                     LRKAVTLTLTAQKKNKTGGWRYSPESTDADSTVTGCQIVSLFAARNAGIPVPDEAFER
                     GLKYMASCRDKKGAYGYTGPAGPRVTLTAIGSLTLSLARLKTDPSFKDSLAYLKKHLN
                     YRDSTYPFYFEYYMSQALFHADQEVWKEWNYKNMRYLGASQAPNGSWLSDHSAAYSTS
                     AALLSLALNYRFLPIYEQ"
     gene            3037..4479
                     /locus_tag="PK077_04835"
     CDS             3037..4479
                     /locus_tag="PK077_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23657.1"
                     /translation="MNNRIRLSLMLAPACLLTAWANSVIDVVTDNNYRLRGEIISYGK
                     QGIVIKHPAMRQNAVILPSGIRALYFTGTASPDLRARDKIFMAAGHRDIIPCNIISIT
                     QDKVQYRDMLGYERSVPRSQVAGFRLNTLQEKGFWQEPLIFDNSWVYSGNREEDYRTQ
                     AGRKLMNALKPKIQGDKYQYRLDNNDYPTWIPLYKNIGLDPSSFTFRTTITMNGNNDR
                     SGIVFCFGGNENIPFRSNSGSNLNRLMLSISPGKCTLLREQKSGILVLGEAAIPAPIL
                     TEGVDIQLTASRQGGNKQVYELLIGDLPPRLITDPSPPEQPLQGDAFGLQMEGHVSLT
                     ISKLTLSSITLSAKSVAKNGNAETDLVLTKEEDAIPGSVSGYDSKTGTLTLSTDKDYP
                     GIPRDLSIPSKYLDTVFFAEQNRKDDSRPPARHSLQMKDGSRLHGDILSMDSLNIILR
                     HPQLGQISLPLKNITRVEFLNPAQPSRSTP"
     gene            4491..5918
                     /locus_tag="PK077_04840"
     CDS             4491..5918
                     /locus_tag="PK077_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23658.1"
                     /translation="MKQAMTLFLCACALPTALLLPHARAEGVITLKSGEKLPGSMDTM
                     NRPYLKVRSSILSKPVDVHMGELDELVSSRPLEIPEQFSIIRLANGDEVYGTLKALDA
                     ENLQLQTAWGGLLQVDRKYVRHIGFDSQKAYLRNATESLQGWESSAKSTLPECRNGYW
                     LMRVSNNTELQTSFPMPPRLHVQFSLYHTNTFRINLALWQDSESGNSINLDLSLEKAE
                     LSKNSSGQYRTLGRVKRNTERNWYADKSVKRSDVHFYADPEKGNYYLYVNGEQVAWWE
                     EVKEMDTIFENESGDDEGEDGESAGKHEFKPGNTLALGGYDSLNMAVFHLNVFDWNGA
                     IPHLEEELDIISRYDPAAPRDKALLVNGDVLRGAISLQEEGSIRIKSDHYDVTIPTVR
                     VRALNQKGHEEKDLPEDSSDTRVFLTDQSVLSLALETIRNGFMEGRSSAVGKVRIPLQ
                     SVRKVQFNLQNPELRKQRETPFRYK"
     gene            complement(5984..6790)
                     /gene="proC"
                     /locus_tag="PK077_04845"
     CDS             complement(5984..6790)
                     /gene="proC"
                     /locus_tag="PK077_04845"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRN23659.1"
                     /translation="MKTGIIGLGKMGGALLRGMLKAGAAVPEEVWVYDHHHENMQALR
                     EEYPGVHEAASEAEAADAVEVLILAVKPHGILPLISSLSERGAELPLLISIAAAVSLD
                     DMEACASDGTRIIRAMPNTPCMVLAGVIAYSTGNGVKDEDEEAARMLLSGCGSVYKVA
                     ESGMNAVSAISGCGPAYMFTALDALADAGVAMGLSRKTALGLAAETMRGASLMLERTG
                     KHPMALRDEVTSPGGTTIAALNALDECGFRNAWIQAVKSAVRRAEEMEER"
     gene            complement(6861..7523)
                     /locus_tag="PK077_04850"
     CDS             complement(6861..7523)
                     /locus_tag="PK077_04850"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HRN23660.1"
                     /translation="MEDIINARCGWAGTDELYIKYHDEEWGRPVSDDKTLFEFLVLES
                     AQAGLSWLTILRKREGYRRAFHGFDVEKVARMTAKDVERLMRFDGIVKNRLKINSAVN
                     NAKLFMAVQEEFGSFYRYVLSFFPNHQPIVNHFRALAQIPAVSPESEAMSRDMKRRGF
                     RFFGPTICYAFFQATGFVNDHIEGCFCNAAQYAKSQPHVQPETLITGPEGTNAEIRHS
                     RP"
     gene            complement(7535..>8350)
                     /locus_tag="PK077_04855"
     CDS             complement(7535..>8350)
                     /locus_tag="PK077_04855"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRN23661.1"
                     /translation="LGLFAVALLALSSFRLSAVLGPETGGINELVLLKNSAYGAMRVF
                     GLFFCVAATALIIPRDAEDRILYTILCKPVPRIDYLMGKVLGVLALTLVAVLLMDAVM
                     TLVLWMRTDTVVAEQIASLKGRYTLEEMQPYLDRIRLQGATWNVQTGLGVMMCEFVVL
                     SSLTLLMSCVTNGTIISALLTFMIYLAGLFQTQALSLWVGSGTDGLSWWEVAGSRLFS
                     LIFPNFQIYSVTDSALNGQFIPLSLFGTLALITLGYFVFHMTLAAWLFRKKEF"
BASE COUNT         2016 a         2559 c         2191 g         1584 t
ORIGIN      
        1 gggtgaaaaa tccttttacg acggcaatga ggatgaaaac ggagcctcag ctccaaaacc
       61 ggccggtgcg gacaaaccgg cgcccggcct ccgcggggag aaagtgctgg cggacggaca
      121 gcccacgcag accgggctga aaggaacatt cctgttccag gccagggcgc tgaagctatc
      181 tccccagcgc gtcgtcatcc gcgggtttac gcaggattac aaaccgggag gcaaacgcgt
      241 ttattcggaa cccatgatta ttttcgtcct ttccaagtcc gagcacgccc agatgattcg
      301 caatgaactg gaacgcatca ccagcgatct ggaaggcatg atccgccgca tggacgccat
      361 ggcggacgaa gcaaaacggc tgaaaggaat ggaaaacggc aaattgaaag atgcggagtc
      421 ccaggaacgc ctgcacgccc tggccgatga agagcaggcc aacaggcgcg agttgggaga
      481 cttgctcaac cgctcggaaa gcctgtttaa ggaagcctcc cgcaattccc agatagaccc
      541 ggccggcatg aaggaattca tgaaaggaat ttccatgctc aaacccattc ccgacgggtc
      601 catgaagatg gcccagaagc tcttccggga ttccgcctcc gagagccgct ccgagcagga
      661 aagcaggaag gacctggacg acggggagaa ttcccattcc gaagccacac aggcgctgaa
      721 agacgccatg aaccagctct ccaagtccgc gcaggacatg gaggccagca cattcgtggc
      781 acggctcagg caggccgcat ccaaggagga ttccatcgcc catgcgctgg ccggccagat
      841 caacaccatt gcgggcatga ccatggagga gcttgaccct tccaccaggc gtgaaatgga
      901 aaccatcgcc acccttcaaa atgcctccac gcaggacata ggctggattc tggaagacct
      961 cagctattac aagtcacgca ccggggagcg catttacagc gacctgtaca accagatgaa
     1021 cgctttctcc ctccgggaga agctggacct ggtgcacggc aatatcctga acgcaatcac
     1081 agcccgctcc attgacgagt cccgcctgta tgcctccact ctgcgccatt gggccaaatt
     1141 gatagacgac tataagaaga gccgccgcgg cggcggggga ggaggaggcg gcgggggagg
     1201 cagccagtcc tccatttccg acgccgagtt tgaattcatg ctgaaactca tccgcatgat
     1261 ccaacaggaa caggatatcc gcatgcgcac ccgcgccgcc gaacaggaac accgaaaaaa
     1321 acttcccccc cctgccccat gaataccgcc aattttccag ccatgattct ggcgctttcc
     1381 ctgccctgcg ccgccgctcc ggctcccgct ccggaatatt ccgccaagca cagggaaacg
     1441 gcatccaaac tggcagtaca acaggacgag ctccaggcgg acacttccga ccttatcctg
     1501 ggagaaacca atgaggaggt agtggacctg ctgaaaaaat gccgccatgc catgaatgac
     1561 gccgtggacc tgctggaagt ttacaatacc ggaggccaga cgctggccgc gcagtcggat
     1621 gtgattgaat tgatttacca ggcagccaag gccaagatga ccggggccga cggccggccc
     1681 aagccgggag gaagagctct tatggacatg ctgcgccagc tgctcggcat ggatagcgac
     1741 tcccagtccg cggaacagca ggaagggaat gggaaagggc aggaaggttc ccagagaggc
     1801 agcggcaaca atcccggacg cggagcgaat ggaaaatcca atatggcctc ctcccaggat
     1861 aaaggcgttt ccaatccgga cacggctgcg gaaacgcgtt ccgttcccaa atccaccggc
     1921 atgtccgcag acgacatgcc ttccgaattc cgcaaggcgc tggacgccta caacaagact
     1981 ttgcagaagt gagacgcatc tctccggaca gccctttcac gcatgaacag caacttcccg
     2041 tttccatgaa aaatccccgc cgcctcctga ccgctttctt gacctgctgc atgacggccg
     2101 caccgcaaaa cgcaacggcc caatccccca tgagaacgtc ttcttccgtg ccgccccagg
     2161 tggaactgat gtatgtgaag gggctacgct atcttcagaa tgcccagaaa acggatggaa
     2221 cctatgatgg aacttacggg agggagcccg gcatcatcgg tttttgcctt atgtccgtgc
     2281 tggctcacgg agacgatccg aacgcggggc cgtacgccac catggtgcgc cgctgcgtag
     2341 attacatcct gtccaggcag aacaaggtat ccggctacat tggggattcc atgtacaacc
     2401 acggcttcgc cactctcgcc ctggcggaag cctacggcat ggtgcgggac gatcgcatag
     2461 gccccgctct ccgcaaggcc gtgaccctga cactgaccgc ccagaaaaaa aacaaaacgg
     2521 gaggctggcg ctattccccg gaatccacgg acgcggacag cacggtgacc ggctgccaga
     2581 tcgtctccct gttcgcggcg cgcaatgcgg gcattcccgt gccggatgag gcctttgaac
     2641 gcggcctcaa gtatatggct tcctgccgcg acaagaaagg cgcctacggc tacaccgggc
     2701 cggcgggtcc ccgcgtcacc ctcacggcca tcggttccct gacgctgtcc ctggcgcgcc
     2761 ttaaaacgga cccgtccttc aaggattccc tggcctacct gaaaaagcac ctgaactacc
     2821 gggattccac ttatccgttt tattttgaat attacatgtc acaggccctg ttccatgcgg
     2881 accaggaagt gtggaaggaa tggaattaca aaaatatgcg ctatctggga gcctcccagg
     2941 cgcccaacgg gtcctggctt tccgatcatt ccgcggcata ttccacttcc gccgccctgc
     3001 tctccctcgc gcttaattat agatttctac ccatctatga acaatagaat ccgcctttcc
     3061 ctgatgctgg ccccggcctg cctgctgacc gcctgggcca attccgtcat cgacgtggtt
     3121 acggataaca attaccgcct ccgcggggaa atcatttctt acggaaaaca gggcatcgtc
     3181 atcaagcacc cggccatgcg ccagaacgcc gtcattctcc cgtcgggaat ccgcgctctc
     3241 tactttacgg gaaccgcctc tccggatctt cgcgcccggg acaaaatttt catggctgcg
     3301 gggcacaggg acatcatccc gtgcaatatc atttccatca cacaggacaa agtgcagtac
     3361 cgggacatgc tgggctatga acgttccgtt ccccgcagcc aggtggccgg gttccggctg
     3421 aatacgctgc aggaaaaggg cttctggcag gaaccgctga tttttgacaa cagctgggtt
     3481 tattccggca accgggagga agactaccgc acccaggcgg ggagaaaact gatgaatgcc
     3541 ctgaagccga aaatccaggg agacaagtac cagtaccggc tggacaacaa tgattacccc
     3601 acctggatac ccctgtacaa gaatatcggc ctggatcctt cctcctttac cttccgcacc
     3661 accatcacca tgaacggcaa taatgaccgc tccggcattg tcttctgttt cggggggaat
     3721 gaaaatattc ctttccggtc caattccggc agcaacctga acaggctcat gctcagcatc
     3781 tcgcccggaa aatgcaccct gctccgggaa cagaagtccg gcatcctcgt gctgggggaa
     3841 gcggccattc cggcccccat tctgacggag ggcgtggata ttcaattgac agcctcccgc
     3901 cagggtggaa acaagcaggt ttacgagctg ttgataggag atctgccgcc cagactgatc
     3961 acagacccca gcccgccgga acagccatta cagggagacg ccttcggcct ccagatggaa
     4021 gggcacgtgt ccctgaccat cagcaaactg accctttcct ccatcaccct gagcgccaaa
     4081 tccgtggcca aaaacgggaa tgcagagaca gaccttgtcc tgacaaagga agaagacgcc
     4141 attccgggtt ccgtcagcgg ttacgatagc aaaaccggaa cgctgacgct ttccacggac
     4201 aaggattatc cgggcattcc ccgtgatctt tccatcccgt ccaagtacct ggataccgtc
     4261 ttttttgcgg aacagaacag gaaagacgac tcccgccccc cagcccgcca cagcctccag
     4321 atgaaggacg gttcccgcct gcacggagat attctgagca tggattccct gaatatcatc
     4381 ctgcggcatc cgcaactcgg acagatttcc ctgccgctga aaaacatcac ccgcgtggaa
     4441 ttcctgaatc ccgcccagcc atcccgttcc actccctgat tgaccccgcc atgaaacaag
     4501 caatgacgct tttcctgtgc gcctgcgccc tgccgaccgc cctccttctg ccccatgctc
     4561 gtgcggaagg ggtcattacg cttaaatccg gggaaaaact gccgggcagc atggatacga
     4621 tgaaccgccc ttacctaaag gtccgttcca gcattctttc caaacccgtg gatgtccata
     4681 tgggggagct tgacgagctg gtctcttccc gtcctctgga aataccggaa cagttttcca
     4741 tcatcaggct ggccaatggg gacgaggttt acggcaccct gaaggcgctg gatgcggaaa
     4801 atcttcaact ccaaaccgct tggggcggtc tgcttcaggt ggaccgcaaa tatgtgcgcc
     4861 acattgggtt cgactcacag aaagcctacc tgcgcaacgc tacggaatcc ctccagggct
     4921 gggagtcttc cgccaaaagc acgctgcccg aatgccgcaa cggatactgg ctcatgcgcg
     4981 tctccaataa tacggagctg caaacttcct tccccatgcc accccgcctc catgtgcagt
     5041 tctcccttta ccataccaat acgttccgga ttaatcttgc cctctggcag gattccgaat
     5101 ccggaaacag catcaacctg gatctctccc tggaaaaagc ggaactttcc aagaacagtt
     5161 ccggccagta ccgaacgctt ggcagggtga aaaggaacac ggagcggaac tggtatgccg
     5221 acaagagcgt caagcgttcc gacgttcatt tttacgccga tccggaaaag ggcaattatt
     5281 acctgtacgt caacggtgaa caggtagcct ggtgggagga agtcaaagag atggacacta
     5341 tctttgaaaa cgagagcgga gacgatgaag gggaagatgg agaaagcgcc gggaaacacg
     5401 aattcaaacc gggaaacact cttgccctgg gaggttatga cagcttgaac atggccgtgt
     5461 tccatttgaa tgtatttgac tggaatggag ccattcccca tctggaggag gagctggaca
     5521 tcatttcccg ttacgacccg gccgccccca gagacaaggc tctgctggta aacggagacg
     5581 tgctcagagg agccatttcc cttcaggagg aaggctccat ccgcatcaag tccgaccatt
     5641 atgacgtcac cattcccacc gtcagggtac gcgcactgaa ccaaaaaggc catgaagaga
     5701 aagaccttcc ggaagacagc tcagacaccc gcgttttcct gacggaccag agcgtccttt
     5761 ccctggccct ggaaactatc cggaacgggt tcatggaagg gcgctcctcc gcagtcggca
     5821 aggtccggat tccgctccaa tccgtcagaa aggtccagtt caacctgcaa aacccggagt
     5881 tgaggaagca gagggaaacg ccgttccggt acaaataaag gctctccacc aggccgtcct
     5941 ttccggagaa agccggctcc gggaaagcca gaagaagcat ccgttaccgc tcctccattt
     6001 cctccgcgcg ccgtacggcg cttttcaccg cctgaatcca ggcattgcgg aagccgcatt
     6061 catccagcgc attcagggcg gcaatcgtgg ttcctccggg ggaagtgacc tcatcccgga
     6121 gagccatggg atgcttcccg gtccgttcca gcatcaggga agccccccgc atggtttccg
     6181 cagccagtcc caaagcggtt ttcctggaca atcccatggc tacccctgcg tctgcaagcg
     6241 catccagagc cgtaaacatg taggcggggc cgcaaccgga aatggcggaa acggcattca
     6301 tcccggattc cgccaccttg taaacggagc cgcagccgga aagcagcatc cgggccgcct
     6361 cttcatcttc atcttttacg ccgtttcccg tactgtaggc gatgacgccg gccaaaacca
     6421 tgcacggggt gttgggcatg gcgcgtataa tgcgcgttcc gtcgctggcg caggcttcca
     6481 tatcatccag ggaaacggcg gcagcgatag aaatcagaag gggaagctcc gccccccgtt
     6541 cagaaagaga ggaaatgagc ggaaggatgc catgcggctt cacggccaga atcaggactt
     6601 ccacggcgtc cgccgcctcc gcctccgagg ccgcttcatg aactccgggg tattcctccc
     6661 tcagagcctg catgttctca tggtgatgat cgtacaccca aacttcctcc ggaaccgctg
     6721 ctccggcttt cagcatgccc cgcaacaggg cgcctcccat ttttccaagt ccgatgatac
     6781 ctgttttcat ggttgatgat tttttacagt caacatataa gcgatttata gaaaatggaa
     6841 atgccaatga aggcccatgg ctatggacga gaatgcctta tttccgcgtt tgttccctcc
     6901 ggccctgtta tcagcgtctc tggctggaca tgcggttggg attttgcgta ctgggccgcg
     6961 ttgcagaaac agccctcaat atggtcgttg acaaacccgg ttgcctggaa aaacgcataa
     7021 cagatcgtcg gaccgaaaaa cctgaatccc cgccttttca tgtccctgct catggcttcc
     7081 gactccggag aaacagcggg aatttgagcc agagccctga aatggttaac aatgggctga
     7141 tgattgggga aaaacgacaa gacgtaccgg taaaaactgc cgaattcttc ctgaacggcc
     7201 ataaacagtt tcgcattatt gacagcgctg ttgattttca aacggttttt gacaattcca
     7261 tcaaatcgca tcaaccgttc cacatctttt gccgtcatcc gcgccacttt ctccacatca
     7321 aagccatgga aggctctccg gtatccttcg cgcttccgga ggatagtgag ccaggataat
     7381 cccgcctggg cactctccag caccagaaat tcaaacaggg ttttgtcatc ggaaacaggc
     7441 ctgccccatt cttcatcatg gtattttatg taaagttcat cggttccggc ccagccgcaa
     7501 cgcgcattga tgatgtcttc catacctgtc cccgttaaaa ttcctttttc ctgaacagcc
     7561 acgccgccag cgtcatgtga aagacgaagt accccagcgt aatcagggcc agggttccga
     7621 acagggaaag gggaataaac tgaccgttca gggcggaatc cgtcacggaa taaatctgga
     7681 aattcggaaa aatcagggaa aagagcctgc tgcccgccac ttcccaccag gaaaggccat
     7741 ccgtgccgga tccaacccac agggaaagag cctgcgtctg gaacagcccc gccagataga
     7801 tcatgaacgt cagaagcgca ctgataatgg tgccattcgt cacgcaggac atcaacagcg
     7861 tgagggagga aagaacgaca aactcgcaca tcatcacgcc cagccccgtc tgcacgttcc
     7921 aggtagctcc ctgaaggcgg atcctgtcta ggtacggctg catttcctcc aacgtataac
     7981 ggcctttcag ggaggctatc tgctccgcaa ccaccgtatc cgtccgcatc cacagcacca
     8041 gcgtcattac ggcatccatc agcagcacgg caaccagggt cagagccagc acacccagca
     8101 ccttccccat cagatagtcg atgcgcggca ccggtttgca aaggatagtg tatagaatac
     8161 ggtcctccgc atccctggga ataatcagag ccgtagccgc cacgcagaaa aagaggccga
     8221 acacccgcat ggcgccgtag gcggagtttt tcagcagcac gagttcattg attcccccgg
     8281 tttccggccc cagcaccgcg ctcaggcgga aggaactcag agccagcaac gccacggcga
     8341 agagcccaag
//
LOCUS       DAOMSU010000079         8159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_650_length_8159_cov_2.857971, whole genome shotgun sequence.
ACCESSION   DAOMSU010000079 DAOMSU010000000
VERSION     DAOMSU010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_650_length_8159_cov_2.857971"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PK077_04860"
     CDS             complement(<1..246)
                     /locus_tag="PK077_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN23662.1"
                     /translation="MSSGMKERLCRSLEKVLHAETRELPAVAGGILLFFLLFLAYSML
                     RPVREMMGIAGGVQNLQWLFTATFAVSIGIQFLFGWIS"
     gene            433..1512
                     /gene="dinB"
                     /locus_tag="PK077_04865"
     CDS             433..1512
                     /gene="dinB"
                     /locus_tag="PK077_04865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRN23663.1"
                     /translation="MNQRKIIHVDMDAFYASIEQRDHPEYRGKPIAVGRPEMRGVVAA
                     ASYEARRFGVRSAMPSMKALKLCPHLIFTRNRMDVYKAVSAQIHAIFHRYTDLVEPLS
                     LDEAFLDVTENKPGIPLAVDIARRIKKEIRRELHLTASAGVSYNKFLAKIASDYRKPD
                     GLFTIHPSRAEKFIAALPIEAFWGVGHATAERMRALSITNGAQLRARDKEFLIRHFGK
                     TGAIFYNFARGVDDRPVEPSRMRKSVGCEETYRENVTRAEALEQRLPLLAEELAGRLA
                     RSGFRGNTLTLKVKFPDFVQKTRCATVPEILTEKEGILPLARTLMEELDSGDRTFRLL
                     GLSVSHPQEEQRQGIWEQLWLELEY"
     gene            1584..2135
                     /locus_tag="PK077_04870"
     CDS             1584..2135
                     /locus_tag="PK077_04870"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF013780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HRN23664.1"
                     /translation="MSKTRSNLVISHMNQEERRHWASWIGMIAEGVAGVMAVNGGIPA
                     SRDVPDPGPARTRRPLTETEKRIIEHQGTEPPFSGEFVSFFEPGIYSCRKCGAPLFRS
                     EDKFDAGCGWPCFDDSLPDAVTSVHAGGGRRTEITCARCGGHLGHIFNGERLTPKNLR
                     YCVNSLSLEFRAAPLPIAADAPR"
     gene            2409..3656
                     /gene="fabF"
                     /locus_tag="PK077_04875"
     CDS             2409..3656
                     /gene="fabF"
                     /locus_tag="PK077_04875"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HRN23665.1"
                     /translation="MTDRRIVITGIGVISPLGNDLASTWEAMKAGRSGIDTIKSMDVT
                     GYSTKIAGEVKDFDPAPYFKTPKDARRVDRFTHFAMGAAGMALKDSGLDLEAEDKTRI
                     GVMVGSGIGGLGTLESQHATLLSKGPARVSPFMIPYMISNIASGLISMEYGLGGPNMS
                     IVTACATSNHNIGEAWRIMKFGDADVMVCGGAEATILPTGVAGFSNMKALSSRNDEPQ
                     RASRPYDVDRDGFVMGEGAGVVILETLEHAQKRGAKIYGELVGYGISADAYHLTAPDP
                     EGRGAARCMKMALEHAGMKPEDIDYVNTHGTSTPLGDICEIKAIKAVFGDHAKNGLLI
                     SSTKSMTGHLLGAAGGVELAACLMAMQDNIIPPTINVDNQDPECDLDCVPNKARETRV
                     DAVLSNSFGFGGHNSSVIVKRFA"
     gene            3744..4193
                     /locus_tag="PK077_04880"
     CDS             3744..4193
                     /locus_tag="PK077_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23666.1"
                     /translation="MPAKTHQDILLMRWALFSVAGLSLLVALYYLGLTPVWARELKEA
                     MASAPFLLPPDAPALSPGTGFLLCMALTLALVYDLLLIRGTLRKTAVLAVFLLLLASF
                     TPVLGLWGLFFNAVPCLLSAGSAGVLAILCPQTPPVRRHSEPPSAHE"
     gene            4186..4794
                     /gene="recR"
                     /locus_tag="PK077_04885"
     CDS             4186..4794
                     /gene="recR"
                     /locus_tag="PK077_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRN23667.1"
                     /translation="MNNLDYPIPVLELVTALKQLPGIGTRGAERMALWMLQGHMGEAE
                     AIARTIGQAAEHVTPCPVCGFFSTEGEPCAACRDEERDSRLICVVEQATDVIPIERSS
                     AYRGLYHCLGGKLSPLDDVEPEDLNIQSLMERVSRQPDCEVILATGSDVEGEATATYL
                     HHLLKEMDCRISRPAQGLPAGSGLSHADTLTLMKALEGRTRL"
     gene            complement(4959..5183)
                     /locus_tag="PK077_04890"
     CDS             complement(4959..5183)
                     /locus_tag="PK077_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23668.1"
                     /translation="MKPYPCQMVQAVAVIVERIADTAFRATLPNGKTAVAFVEKKNAS
                     LRELLKPGDRVNVTICPADFDRARIDGMAE"
     gene            complement(5197..>5560)
                     /locus_tag="PK077_04895"
     CDS             complement(5197..>5560)
                     /locus_tag="PK077_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23669.1"
                     /translation="DVFSVSRQGAGGNLAWANVNIGGTLDNPSEDLSARLIKAAGNRL
                     TEIGMGKAAEVADVAARLLNRGTGENGAGDGAERNGRKFRIPAGDGVPVPVVPRLQDA
                     AEKGLKTGNDLMNELMNW"
     assembly_gap    5561..5570
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5571..6763)
                     /locus_tag="PK077_04900"
     CDS             complement(<5571..6763)
                     /locus_tag="PK077_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23670.1"
                     /translation="MSRIRRIRRKKPRTGSVWKKLLLWGVLGLLVLAAAAVAGSYLYV
                     RAYLKSDGFLAMLEQSAVDDMNVETARIDPLDWDGSGIRCGGVVMEGHEFLTSLQARN
                     IETEFSRWELLKRAFVITSVNIAELKLHLAPVSFRFREKPEGPRSWAEENILPDTFRL
                     EKGSIDSLSVSYGEAGRLYVLDGARVESAYDAGSSQYRFDVRGGKLLLPFQGCPEFSL
                     MSGTAQFNHSSRRVNIPSCRLTTDAGGYLDIKGDWDGSSSSWTANVVVNGVPVSSILE
                     DHWRKHVHGSVSGGIDLRGGRDGVTHVAGLARLQGGMLTGLPVLDRLALFCGSSRFRN
                     LPLHEASAQFVYQESAWHISNILVESENLVRVEGWLEIGKGGALTGRLQVGLRADGLW
                     KALPG"
     gene            complement(6866..>8159)
                     /locus_tag="PK077_04905"
     CDS             complement(6866..>8159)
                     /locus_tag="PK077_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23671.1"
                     /translation="AQTFFSFTGMYGDGSALLSDNGLLWIVGGVILLNLGIIYRGISK
                     GIEMFSRWFMPLLLLISLVLLVRILCIGTPDPSYPDRSIEQGLGYMWNPSKVLVEELD
                     RDSGDWKTVSMVSASHAGALDEAVRQVEMSGGTRRLTEVTLWDGLKNIELWIAAAGQV
                     FLSLSVGTGLILTYASYVRKKEDIALSGFSAAASNEVCEVGIAGMMTVPAAVAFLGVA
                     GAAGQGTFALGFMVLPQAFAKMGSSVVFGSLFFLLLTVAAVTSSISMMQVGLSFIEEF
                     MGLKRKMAVVVQGFFTATGTFIVAWYSGNLLAMDTYDFFLGTLCFFVSAMVMMILFSW
                     KLGVDRGLKDLEDSSVIRIPRIYRFIMKFVTPTLLLAIFLAWLAQNIWVKQAAPIEAL
                     GRGEHGAVIPMGFLAAYALFLLFITMASGRHKVYHGPR"
BASE COUNT         1766 a         2718 c         2122 g         1543 t
ORIGIN      
        1 ggaaatccat ccgaataaaa actggatccc tatggaaacg gcgaaagtag ccgtaaaaag
       61 ccactgcaga ttctgaacgc ctccggcaat gcccatcatc tcccggaccg gcctcaacat
      121 ggaataggcc aggaacaaca ggaaaaacag gaggattccc cccgctacgg caggcaattc
      181 gcgggtttcc gcatgcagca ccttttccag gctccggcac aggcgttcct tcattccgct
      241 ggacatgccc ggaatttacc atgcccggaa gattttttca cttaaatcgt cccgttcccc
      301 aaaaatccgc ctttctttca cctccccgcc ggaacttctc cttccaactg gacttgcagg
      361 ataagccgga ttatgatggg gcatcccatt ccctcatgtt tcccgcccca tcaggaatca
      421 tgaagcgccc ccatgaacca gagaaaaatc atccatgtgg atatggatgc cttttacgca
      481 tccatagaac agcgggacca tcccgaatac cgcggcaagc ccatcgccgt aggcaggccg
      541 gaaatgcgcg gcgtggtggc ggcggccagt tacgaggcgc gccgtttcgg agtgcgttcc
      601 gccatgcctt ccatgaaggc tctcaagctt tgcccccatc tgattttcac ccgcaaccgc
      661 atggatgtgt acaaggccgt ctccgcgcag atacacgcca ttttccaccg ttacacagat
      721 ctggtggaac ccctttccct ggatgaagcc tttctggacg tcacggaaaa caagccgggc
      781 attccgctcg ccgtcgacat tgcgaggagg attaagaagg aaatccgacg ggaacttcac
      841 ctgacggcct ccgccggcgt ttcctacaat aaattcctgg caaaaatcgc ttccgattac
      901 cgcaagccgg acgggctgtt cacaatccat ccatcccggg cggaaaaatt catcgcggca
      961 cttcccattg aagctttctg gggagtcggg cacgccaccg ccgaacgcat gcgcgccctt
     1021 tccatcacca acggggcgca gctccgggca cgggacaaag aattcctgat aaggcatttc
     1081 ggcaaaacag gagccatctt ctacaacttc gcccgcggtg tggatgaccg ccctgtggaa
     1141 ccttcccgca tgcgcaaatc cgtgggttgt gaagaaacct accgggaaaa cgtcaccagg
     1201 gcggaagcgc tggaacaacg cctccccctg ctggcggaag aactcgcggg gcggctggcc
     1261 cgttccggct tccggggaaa cacccttacc ctgaaggtta agttcccgga ctttgtccag
     1321 aagacccgct gcgcgaccgt tccggaaatc ctgacggaga aagaaggaat cctccccctg
     1381 gcccgcaccc tgatggaaga actggattcc ggggaccgta cattccgcct tctggggctg
     1441 tccgtctccc atccccagga agaacagcgg cagggcatct gggaacagct ctggctggag
     1501 ctggagtatt aactcttcca tagactgcca tattttcagg gctcccccct taaaaacggc
     1561 aatatcaaga tattgtttca ggaatgagca aaacccgctc caacctggtc ataagccata
     1621 tgaatcagga agaacggaga cactgggcgt catggatagg catgattgcg gaaggcgtgg
     1681 ctggagtcat ggccgtgaat ggagggattc ccgcctcccg ggacgtaccc gatcccggcc
     1741 ctgcccgcac gcgccgcccc ctcacggaaa cggaaaaacg cattatcgaa caccagggaa
     1801 cggaaccgcc cttttccggg gaattcgtca gcttctttga gccgggcatc tattcctgcc
     1861 gcaaatgcgg tgcgcctctg ttccggtcgg aagacaaatt cgatgccgga tgcggctggc
     1921 cctgttttga tgattccctg ccggacgccg tcacaagcgt ccatgcgggc ggcgggcggc
     1981 gcacggaaat cacatgcgcc cgctgcggag gccatctggg acacattttc aatggagaac
     2041 gcctgactcc gaagaatctg cgctactgcg tcaattccct ttccctggaa ttccgggcag
     2101 cccctcttcc gatagcggcg gatgcccccc gttaaaggcg cgcggcaggg aaaaatatcc
     2161 ggccgccctt ccgtgcgctg ccggatgcgg ccgccgcggc ggaatttacc ctgaaatctg
     2221 ttcacggccc cggagcctca gcaggcaaca gggaatatcc ttgacccgca aaatccttgc
     2281 cgtaagcagg atacgccttt ccgcccaacc tgtttccccc gtgccgggaa acagggggga
     2341 ttagcttgac tttcccgcaa tgtcgcctat tctggttcaa gcattatttg caaccactca
     2401 cacttattat gaccgaccgc agaattgtta tcaccggcat cggagtgatc agccccctgg
     2461 gcaatgatct cgcatccacc tgggaagcca tgaaggctgg ccgcagcggc attgacacca
     2521 tcaaatccat ggatgttacc ggctactcca cgaaaatcgc cggtgaagtc aaggatttcg
     2581 atcccgcccc gtacttcaag acgcccaagg acgcccgccg cgtggaccgc ttcacgcact
     2641 ttgccatggg cgctgccgga atggctctca aggattccgg gctggatctg gaagcggagg
     2701 acaaaacccg catcggcgtc atggtgggca gcggcatcgg cggcctcggc acgctggaaa
     2761 gccagcatgc cacgcttctt tccaaagggc ctgcccgcgt gtcccccttc atgattccgt
     2821 acatgatcag caacattgcc tccggcctca tctccatgga atacggcctc ggcggtccga
     2881 acatgtccat cgtcacggcc tgcgccacct ccaaccacaa catcggggaa gcctggcgca
     2941 tcatgaaatt cggggatgcc gacgttatgg tctgcggcgg agcggaagct accatcctgc
     3001 ccacgggcgt ggccgggttc agcaacatga aggccctcag ctcccgcaat gacgagcccc
     3061 agcgggcctc ccgcccgtac gacgtggacc gcgacggctt cgtcatgggc gaaggcgccg
     3121 gcgttgtcat tctggaaacg ctggaacacg cccaaaagcg cggcgcaaaa atttacgggg
     3181 aactcgtcgg ctacggcatc tccgcggacg cctaccacct caccgccccg gacccggaag
     3241 gccgcggcgc cgcccgctgc atgaagatgg ctctggaaca cgcgggaatg aagccggaag
     3301 acattgacta cgtcaacacg cacggcacct ccaccccgct gggcgacatc tgcgaaatca
     3361 aggccatcaa ggccgttttc ggcgaccacg ctaaaaacgg gctgcttatc agctccacca
     3421 aatccatgac cggccacctc ctgggcgccg cgggaggcgt ggaactggcc gcctgcctga
     3481 tggccatgca ggacaacatc atcccgccca ccatcaacgt ggacaaccag gatccggaat
     3541 gcgacctgga ctgcgtgccc aataaggccc gtgaaaccag ggtggacgcc gtcttgagca
     3601 attccttcgg cttcgggggg cataactcct ccgtcatcgt gaaacgattc gcctgaccat
     3661 ttcaggcctc tcctttccgg cggaagcgct gatgtttgcg gccaccgcag ccatcgcgct
     3721 tccgcttttt tcatttaaaa gccatgcccg ccaaaacgca ccaggacatt ctgctcatgc
     3781 gctgggccct gttctccgta gccgggctta gcctgctcgt ggcgctctat tacctgggcc
     3841 ttactcccgt ctgggcccgg gagctgaagg aagccatggc ctccgccccg tttctgcttc
     3901 ccccggacgc ccccgccctt tctcccggca cgggattcct gctgtgcatg gcgttgacgc
     3961 tggccctcgt ttacgatttg ctgctcatcc gcggcacgct ccgcaaaacg gccgttctgg
     4021 ccgttttcct ccttcttctg gccagcttta ccccggtgct ggggctgtgg ggtcttttct
     4081 tcaatgccgt cccatgcctg ctttccgcag ggagcgcggg cgtgctcgcc atcctctgtc
     4141 cccaaacgcc tcctgtccgg cgtcattccg aacctccttc cgctcatgaa taatctggac
     4201 tatcccatcc ccgtgcttga actggtaacc gccctgaaac agcttccggg catcggcacc
     4261 cgcggcgcgg aacgcatggc cctgtggatg ctccaggggc acatgggaga agcggaggcg
     4321 attgcccgca ccatcggcca ggcggcggaa cacgtcacgc cctgccccgt ctgcggcttc
     4381 ttcagcacgg aaggggaacc ctgcgccgcc tgccgggatg aagaacggga cagccgcctc
     4441 atctgcgtgg tggaacaggc cacggacgtc atccccattg aacgcagcag cgcctaccgc
     4501 ggattgtacc actgcctggg cggcaagctt tcccctctgg atgatgtgga gccggaagat
     4561 ctcaacattc aatccctgat ggaacgcgtc tcccggcagc ctgattgcga agtcattctg
     4621 gcaacaggct ctgacgtgga aggggaggca acggccacct acctccacca tctgctcaaa
     4681 gaaatggatt gcaggatatc ccgccctgcc caggggctgc ctgccggctc cggcctcagc
     4741 cacgcggaca cgctgacgct gatgaaggct ctggaaggac gcacccggct gtaaaggaaa
     4801 gcccttcccg catccatccc ggcacgggga aattcccgcc catgcccccg ttcttcaccg
     4861 tccggaatcc cgtcatgggg acagttccgt tttacagcgg ttttcctgcc tcgcgcgatg
     4921 cctctttccc ccaatgatga agggaaaaac tcaccgcctc attccgccat gccgtctatt
     4981 cgggcgcggt caaaatccgc cgggcaaatc gtcacgttca cccggtcgcc cggcttgagc
     5041 agctccctca gggaagcgtt ctttttttct acaaaagcca cagcggtttt gccattcgga
     5101 agggtggcgc ggaaagccgt atccgcgata cgctccacaa taacggcgac cgcctgcacc
     5161 atctgacagg gataaggttt catatgaaaa aacgggttac caattcatta attcgttcat
     5221 caggtcattc ccggttttaa gccctttttc cgcagcgtcc tgcagccgcg gcacaaccgg
     5281 gacaggtact ccgtccccgg cgggaatcct gaacttgcgg ccattacgct ccgctccatc
     5341 ccccgctccg ttttccccgg ttcccctgtt cagcagccgc gccgcgacat ccgccacctc
     5401 agccgccttg cccattccta tttccgtcag gcggtttccc gccgccttga tgagccgcgc
     5461 ggacaagtcc tccgacggat tgtccagcgt cccgccgata ttcacattgg cccacgccag
     5521 attgcctccc gctccctggc gggaaacgga aaacacatcg nnnnnnnnnn aaccccggaa
     5581 gagctttcca caggccgtcg gcccgcagcc ccacctgcag ccttccggtc agcgcccccc
     5641 ctttcccgat ttccagccag ccttccaccc tcaccaggtt ttcgctttcc accagaatat
     5701 tggaaatatg ccaggcggat tcctgataga cgaactgggc ggacgcctcg tgcaggggaa
     5761 gattccgaaa tctggaagaa ccgcagaaca gagccagcct gtccagaacg ggaaggccgg
     5821 tcagcatgcc gccctgcaac ctggcgagcc cggccacatg ggttacgccg tcccgtccgc
     5881 cgcgcaggtc tattccgccg ctcacgctgc catggacatg ctttctccaa tggtcttcca
     5941 ggatggaaga gacagggaca ccgttcacca ccacattcgc cgtccaggag gaggaagagc
     6001 cgtcccagtc ccccttaatg tccaaataac cgccggcatc cgtagtcagg cggcaggacg
     6061 gaatgttcac ccttctgctg gaatggttaa actgcgctgt accggacatc agggaaaact
     6121 ccgggcatcc ctggaaagga agaagcagct ttcccccgcg cacatcaaac ctgtactggc
     6181 tggaaccggc gtcataggcg ctctccacgc gggccccgtc caaaacatac agccggccgg
     6241 cctccccata ggaaacggac agggaatcaa tgctcccctt ttccagacgg aacgtatccg
     6301 gaagaatatt ctcttccgcc cagctcctgg gcccttccgg cttttccctg aaacggaaag
     6361 acacgggagc gagatgaagc ttcagctccg caatattgac ggaggtaatg acaaaagcgc
     6421 gcttcagcaa ttcccagcgg ctgaattccg tctcaatatt cctggcctga agggaagtca
     6481 aaaactcatg cccctccatt actacgccgc cgcaccggat gccggaaccg tcccagtcaa
     6541 ggggatctat cctggctgtc tccacattca tatcatcaac ggcggactgt tccagcatgg
     6601 ctaaaaaacc atcgctcttc aagtaggccc tgacatacag ataagacccc gccaccgcag
     6661 cagcagccag aacaagcagc cccagcacgc cccacaacag cagttttttc cagacgctgc
     6721 cggtacgggg ctttttcctg cgtatcctcc ttattctgga catgatttca gcctatatca
     6781 aatccggcac cggtcaagcc tccggattgc ctcccaactt gccgcagact gcgggaaacg
     6841 ggcatccgcg cagtcctttc cgcgcctatc gcggaccgtg gtacacctta tgccttccgg
     6901 acgccatggt gatgaaaagc agaaacaggg cataagccgc cagaaacccc atgggaatga
     6961 ccgccccatg ttccccccgc cccagcgctt caatgggcgc ggcctgcttc acccagatat
     7021 tctgggccag ccaggccagg aaaatagcca gcagcaacgt aggcgtcaca aacttcatga
     7081 tgaaacggta aattctggga atgcggatga cggaactgtc ctccagatcc ttcaatcccc
     7141 ggtccacccc cagtttccag gaaaaaagaa tcatcatcac catggcgctc acaaagaagc
     7201 acagcgtgcc cagaaaaaaa tcataagtat ccatcgccag cagattgccg ctgtaccagg
     7261 caacgataaa cgtgcccgta gccgtaaaaa agccctgcac caccacagcc attttgcgtt
     7321 tcagccccat gaattcctca ataaaggaca ggcccacctg catcatggaa atggagctgg
     7381 tcaccgccgc caccgtcaga agcaggaaaa acaggctgcc gaataccacg ctggacccca
     7441 ttttggcaaa agcctgcggg agcaccatga agcccagcgc gaaggtcccc tgcccggcgg
     7501 ctccggccac tcccaggaac gccacggcgg caggaaccgt catcatgccg gcgatgccca
     7561 cctcgcacac ttcattggag gcggcggccg aaaagccgct cagcgcgata tcctctttct
     7621 tcctgacata gctggcatac gtcagaatca gccccgttcc tacggaaagg ctcaggaaaa
     7681 cctgtccggc agcagcgatc caaagctcaa tatttttcag gccgtcccac aaggtcacct
     7741 cagtcagcct gcgggttccg ccggacatct ccacctgcct cacggcttca tccagggctc
     7801 ccgcatggga ggccgatacc atggaaaccg ttttccaatc cccgctgtcc cggtccagtt
     7861 cctccaccag cactttgctg ggattccaca tatatcccag tccctgttcg atgctgcggt
     7921 ccggataaga agggtccggc gtgccgatgc acaaaatgcg caccagcagc accagggaaa
     7981 tcaaaagcag caacggcatg aaccagcgcg aaaacatttc gatgcccttg ctgatgcccc
     8041 ggtaaataat ccccaggttc agcagaataa ccccgccgac gatccagagc agcccgttgt
     8101 cggacagcag ggcgctcccg tccccgtaca tccccgtgaa actgaaaaag gtctgcgcc
//
LOCUS       DAOMSU010000080         8141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_652_length_8141_cov_2.387831, whole genome shotgun sequence.
ACCESSION   DAOMSU010000080 DAOMSU010000000
VERSION     DAOMSU010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_652_length_8141_cov_2.387831"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            210..827
                     /locus_tag="PK077_04910"
     CDS             210..827
                     /locus_tag="PK077_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23672.1"
                     /translation="MKYSIFLTGCIFHFYLCSASYGDTCQINGLEPEVLKSLSYEPPN
                     KELLSTGQNQKAWQTFIENYEIPIRKVQDWYNSIVDKQSADAAADSFNKDVLPILKKL
                     TGRAADMKKQALAIPASSEMQKFIFLRISQLQGRLLIIFLPMQNEEKASPETCYRGST
                     KLFEIILGLTSPSMIDISKFIESQNESQESFETVFKESWPETPSA"
     gene            906..1520
                     /locus_tag="PK077_04915"
     CDS             906..1520
                     /locus_tag="PK077_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23673.1"
                     /translation="MKYSIILTFASSLLIYDSTCYGDACQINGLSQEELKNLSYDPPR
                     NELLSEAQDKKAWQTFVETYEIPVGKVQNWYNSIVDKNSADAAAKSFSTEVSPILKKL
                     TGPAADMKKQVLSIPASSEMQKFILLRILQLQSRLLVIFLPMQSEREMDDNTCYRGSD
                     LLFDTILEPTAQSALDVAKFIDSRNESQEHIEASFKEPWFTSAE"
     gene            1565..1708
                     /locus_tag="PK077_04920"
     CDS             1565..1708
                     /locus_tag="PK077_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23674.1"
                     /translation="MKSIFPESRPLLEQYRELGLADISPEQRLILKGRGRLLVDAIAA
                     ELC"
     gene            complement(2003..2782)
                     /locus_tag="PK077_04925"
     CDS             complement(2003..2782)
                     /locus_tag="PK077_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23675.1"
                     /translation="MPDEGPLEHYLTVSHEPEPDIPEPAPERSPGPWRERGIMLGLGF
                     LLGCIGAYALVYSEMSGGMRSLKQSLSEERDLNASLQKERAHLRETLEQARMDALLEE
                     RSPLSVTVGGIPKTSRRPVPQPPPLTSLSSGGLASPSSAPVPGSLDDRLVNPGPEDWK
                     ERYQYAALKYNQVVEDYNRLKKLYMNLVGRSGAAGGLVTGAQFYKALRKDTRAEARKL
                     DERYMKGGMRGSELRELERSRDEMRHRDWWLKKMARQFQID"
     gene            complement(2819..>3890)
                     /gene="serS"
                     /locus_tag="PK077_04930"
     CDS             complement(2819..>3890)
                     /gene="serS"
                     /locus_tag="PK077_04930"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRN23676.1"
                     /translation="SSIEARVREIGDRIRELDRVAEENAAQLTSLLMNIPNLPHLECP
                     VGADETANPEIKLWGEKPAIENPKDHVELAANLGMISFEDGARITGSGFVVYRGAGAR
                     LERALINFLLNTQTVENGYQEVGVPFVVKRECMEGTGQLPKFEEDMYGTEDQQMFLIP
                     TAEVPVTNLYRDTILQESDLPIKMAAYTPCFRREAGSAGRINRGMIRVHQFDKVELVQ
                     ILKPEDSFRELEVLRSHAESILQKLGLHYRVIELCTGDLGFSSAKTYDIEVWAPGQGQ
                     YLEVSSCSCFTDYQARRMRLRYKDADGRNQFCHTLNGSGTALPRLYVALLETYQQPDG
                     SIRIPEALVPYFGADCIRPEQA"
     assembly_gap    3891..3947
                     /estimated_length=57
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3948..4093)
                     /locus_tag="PK077_04935"
     CDS             complement(<3948..4093)
                     /locus_tag="PK077_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRN23677.1"
                     /translation="MLDIRVIRENPEEVKARLKPRSGDAWKLVDDVLACDEARRNAET
                     EKQA"
     gene            4454..5518
                     /locus_tag="PK077_04940"
     CDS             4454..5518
                     /locus_tag="PK077_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HRN23678.1"
                     /translation="MQQTARAAVLTAPKTFEIREYPIPAIGDDEMLIKVEACGVCGTD
                     GHEYNRDPFGLCPVVLGHEGTGEIVAMGRNITKDTAGNPVALGDKIVTCIIPCGTCDA
                     CLNTPARTNLCENVGVYGLMPDDDVHLNGYFGEYLVIRKGSTFFNVSGMTLDQRILVE
                     PAAVVFNSVVLVQGCGPIGLLQIATLRTLGIETIIAVDGNDSRLELAREMGASRTYNF
                     TRYADLNELLDAVKKDNGGRLADFVFQCTGVGKAGANAWKFVKRGGGLCEVGFFMDGG
                     ESVINHHYDLCNKEVTAVGSWVYSPQDYPTTFDFLKRAYGIGLPLTKLISHRFKLDEI
                     AEALETNVQMKGIKIAVICG"
     gene            complement(5617..6066)
                     /locus_tag="PK077_04945"
     CDS             complement(5617..6066)
                     /locus_tag="PK077_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23679.1"
                     /translation="MEEQCDDLVIDCLQGMVGELGCQVTVRRREHILTLLRELNHLYD
                     CGIPAREMICEEDEEYCFSWIYEFDFYLGDADYLLHFVVDAALNSNGIVNKYLTLERQ
                     VYRKGLCLKASEPMVLDPDALERLKEKIVSAMACPPNTGRFLDWDDD"
     gene            complement(6149..7006)
                     /locus_tag="PK077_04950"
     CDS             complement(6149..7006)
                     /locus_tag="PK077_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HRN23680.1"
                     /translation="MPKIALIQLSVDARPSVNKEKTESAIREAAANGAQIVCTQELFT
                     TEYFCRTEECDLFDLAEPVPGELTEAHRKLAAELGVVIVASGFEKRATGLYHNTAWVV
                     DADGSFLGMYRKMHIPQDPGFEEKFYFTPGDLGYKAFDTKFGRIGVLICWDQWYPEAA
                     RLTAMQGAEIIFYPTAIGWLPEEKPLLGEQQHCAWETVQRGHAVANGCYVCAVNRVGT
                     EGESEFWGQSFVADYYGQIVAKAPVSDEAILYADLDLDALEDHRRIWPFFRDRRIDSY
                     GGITERWGR"
     gene            7110..>8141
                     /locus_tag="PK077_04955"
     CDS             7110..>8141
                     /locus_tag="PK077_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRN23681.1"
                     /translation="MEPVTPTLPDDDVAAIDRLGAIYKELSSELSKMIIGQQRVVELL
                     SIALFSRGHALLMGVPGLAKTLLVSSVAKTLDLSFNRIQFTPDLMPADITGTDIIQDD
                     SAGGKRQFEFIKGPVFANIVLADEINRAPAKTQSALLEAMQERRVTVMGRTFTLLLPF
                     FVLATQNPIEQEGTYPLPEAQLDRFMFLIEVDYPTEEEEMRIARETTGTRSEELKHIL
                     SGEEIIFYQDLVRRVPVPEHIYEYAVKLVRATRPTLETSPEWVKQYVAWGAGPRAVQF
                     LILGAKTRAALQGSYIVRREDIDAIVEPVLMHRVVTNFAAESQGITPRKAVARLAAKI
                     LPPSRHLQSP"
BASE COUNT         1871 a         2375 c         2025 g         1813 t
ORIGIN      
        1 aacaacagcc atggatattt caaaatttat tgaaactcaa aatgaagacc aggaaagcat
       61 tgaagcagct ttcaaagaac catggacaga gacatcatct tcttaaaaag caggttacat
      121 agaaattgcg ttgatctcta ttgaatttcc atccttcttt tattcccctt cattcaggaa
      181 ccctgtcatg cttccataat ctgatgatct tgaaatactc aatcttcctc accggttgca
      241 tcttccattt ttatttatgt tcagcatcct atggggatac ctgccaaatc aatggactgg
      301 agccggaagt actgaaaagc ctttcttacg aaccgcccaa caaggaattg ctctccaccg
      361 gacaaaatca aaaagcatgg cagactttca ttgaaaacta tgaaatccct atccgtaagg
      421 ttcaagattg gtataattcc attgtcgata aacaatctgc cgatgccgca gccgattctt
      481 tcaacaagga tgttctgcct attcttaaaa aactaacggg acgagctgca gacatgaaaa
      541 aacaggctct tgctattcca gcttcttctg aaatgcagaa attcatattc ttacgcattt
      601 ctcagctgca gggacgactg ctaattatct tcctgcctat gcaaaatgaa gaaaaggcga
      661 gtccagaaac atgctaccgt ggaagcacaa aactttttga aattattctg ggattaacat
      721 ccccatcaat gattgacatt tcaaaattta ttgaatctca gaacgaatca caggaaagtt
      781 ttgaaaccgt tttcaaggaa tcatggcctg aaactccatc tgcttaaaaa gcagtttaca
      841 gcagaattgt gctgattacc atttcctccc ctttccgttc catcatgatt caatacctga
      901 taatcatgaa atattccatc atcctcacat ttgcatcttc cctccttatt tatgattcaa
      961 catgctatgg agacgcctgc caaattaacg gtctgagcca ggaagagttg aaaaatctct
     1021 cttatgaccc tccaaggaat gaattgcttt ctgaggcaca agataaaaag gcatggcaga
     1081 cattcgttga aacctatgaa atacctgtag gcaaggtaca gaattggtat aattccatcg
     1141 tggataaaaa ttccgcagac gcagcggcaa aatctttttc tacggaagtt tcacctatcc
     1201 ttaaaaaact tacaggaccc gctgccgata tgaaaaagca ggttttatct attcctgctt
     1261 catccgagat gcaaaaattt attcttttgc gcattcttca gcttcaaagc cgtctgctgg
     1321 tcattttcct acctatgcag agtgaaaggg aaatggacga caacacctgt taccgaggca
     1381 gtgatcttct tttcgatact attctggaac ctacagctca atcagccctg gacgttgcca
     1441 aattcattga ttcccggaat gaatcccaag agcatataga agcttcattt aaagagccct
     1501 ggttcacatc tgccgaataa attattaata gtgcaactat tctaaccttt atttggcgca
     1561 ggatgtgaaa agcatctttc cggaatcccg tcctctgctg gaacaatacc gggaactggg
     1621 cctggcggac atttccccgg aacagaggct catcctgaaa ggccgcggac gcctgctcgt
     1681 ggacgccatc gcagcggaac tgtgctgacg gatagttttt ccgcctcaga gatcagcagt
     1741 ctgcggaatc cgatgcgaac cgaacactga aataagcggg catggtctac tttgttccct
     1801 gattttcaat atccaaggag catcccgcaa aaaaagatac cggtatctgc gtattcggat
     1861 tttgtttttt tatcccggct ctttgggaaa gggatttctt tttgtgaccg ttcggctctc
     1921 tgcaaaaaga ttccatgcaa cgggaatttc agcaaggggc gtttcacaaa aacaatcctt
     1981 cgcgccggca acacccacgt atttaatcaa tctggaactg gcgtgccatt tttttcaacc
     2041 accagtcccg gtgcctcatc tcgtcccggc tgcgttccag ttcccgaagc tcgctgcccc
     2101 gcatccctcc cttcatatag cgttcatcca gcttgcgcgc ctccgcacgg gtatctttcc
     2161 gcagagcttt gtaaaactgg gcccccgtca ccagcccgcc ggctgcaccg ctccgaccca
     2221 ccaggttcat gtaaagtttc ttcagcctgt tgtaatcctc caccacctgg ttatatttca
     2281 aagcggcgta ctggtaacgc tccttccagt cctccggccc cggattgacc agacggtcat
     2341 ccaaagaacc gggaaccggc gcggaagacg gggatgccag ccctccggag gataatgacg
     2401 taagcggcgg aggctgggga acgggcctgc ggcttgtctt gggaataccg ccgaccgtca
     2461 cggaaagggg ggagcgttcc tccagcagag cgtccatgcg tgcctgttcc aacgtttccc
     2521 gcagatgggc acgttctttc tgcagggacg cgttcaagtc gcgttcttct gaaagcgact
     2581 gcttcagcga ccgcatgccg ccggacattt ccgagtaaac cagcgcataa gccccgatgc
     2641 accccagcag aaagcccagg cccagcatga ttcccctttc ccgccacggt ccaggactgc
     2701 gttccggagc cggttccgga atgtccggtt ccggctcatg ggaaacagtc aggtaatgtt
     2761 ccagagggcc ctcgtcaggc atgggctgat tcagggttgg gacgggagaa acgtcggttc
     2821 aggcctgttc cgggcggatg cagtccgcgc cgaaatacgg cacaagcgcc tccggaatcc
     2881 ggatggagcc gtccggctgc tggtaagttt ccagaagggc cacatacagc cgcggcagag
     2941 ccgtgccgga accgttcagg gtgtggcaga actggttcct gccgtcggca tccttgtaac
     3001 gcaggcgcat gcggcgcgcc tggtagtccg taaagcagga acaggaggaa acttccagat
     3061 actgcccctg tcccggagcc catacttcaa tatcatacgt cttggcggag gaaaaaccga
     3121 ggtctcccgt gcagagctca atcacccggt agtgcagccc cagtttttga aggatggatt
     3181 ccgcatggct gcggagcact tccagctccc ggaaggagtc ttccggcttt aaaatctgca
     3241 ccagttccac cttgtcaaac tggtggacgc ggatcatgcc gcggttgatg cggccggcgc
     3301 ttccggcttc ccggcggaag cagggcgtat acgcggccat cttgataggc aggtcgcttt
     3361 cctgtaaaat ggtatcccgg tacaggttgg tgaccggcac ttccgccgtg ggaatcagga
     3421 acatctgctg gtcttccgtg ccgtacatgt cttcctcaaa tttaggaagc tggccggtgc
     3481 cttccatgca ctcccttttc accacaaacg gaacgcccac ttcctgataa ccgttttcca
     3541 cggtctgcgt attcagcagg aagttgatca gggcgcgttc cagacgggcg cccgccccac
     3601 ggtacaccac aaaaccggag cccgtaatcc gcgctccgtc ttcaaaggaa atcatgccca
     3661 gattcgcggc cagttccaca tggtccttcg ggttttcaat ggcgggcttt tctccccaca
     3721 gcttgatttc cgggttggcc gtttcatccg cgcccacggg acattccagg tgcggcagat
     3781 tgggaatgtt catcagcagg gaggtgagct gggctgcatt ttcctccgcg acacggtcca
     3841 gttcccggat gcggtccccg atttcgcgca cgcgcgcttc aatagaggac nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag gcctgctttt
     3961 ccgtttcggc attgcggcgc gcctcatcgc aggccagaac gtcatcaaca agcttccacg
     4021 cgtcgccgct gcggggcttt aaccgcgctt tcacttcttc cggattttct cgtattacac
     4081 ggatgtcgag cataacgcgc acagcttgca ctccatcccc ccgccacgca agccaaatga
     4141 acggccttcc atggaaaaac gttacccttt cacggaaaaa cgctgcgcgc agaggcatgc
     4201 tttcccgaat gccgcccctt tccggaaact accgtttttc ccgtgtcaga caatgatttt
     4261 tttccttctc aggaaaaccg tacagcagga ggaccggact ctccatgttt ccgggaatca
     4321 tggacatccg gcgccagggt gtattgcagg aaggaccggg aaggaaattc ctcccgtttt
     4381 caacaggcgg agttcctttc atcactattt gttcttgaag aatccgggcg gctaaggata
     4441 gtgtaaggac atcatgcaac aaaccgcacg cgccgctgtc ctgacagcac caaagacatt
     4501 tgaaatccgt gaatatccca ttcccgccat cggagacgat gaaatgctga tcaaggtgga
     4561 agcctgcggc gtttgcggaa cggacggcca cgaatacaac cgggacccct tcggcctctg
     4621 ccccgtggtt ctgggccatg aaggcaccgg ggaaatcgtc gccatgggca ggaatatcac
     4681 taaagacacc gccggaaatc ccgtagcgct gggggacaag atcgtcacct gcatcattcc
     4741 ctgcggcacc tgtgacgcct gcctgaatac tccggcccgc accaacctgt gcgaaaatgt
     4801 gggcgtgtat ggcctgatgc ctgacgacga cgtgcatctg aacggctact tcggggagta
     4861 cctcgtcatc cgcaagggct ccacattttt caatgtttcc ggcatgacgc tggaccagcg
     4921 cattctggtg gaacccgccg cagtggtgtt caattccgtg gtgctcgtgc agggctgcgg
     4981 ccccatcggc cttcttcaaa tcgccacgct gcgcacgctg ggcattgaaa ccatcatcgc
     5041 tgtggacggc aatgactccc gcctggaact ggccagggaa atgggagcct cccgcacgta
     5101 taatttcacc cggtacgcgg atctgaacga actgctggat gccgtgaaaa aggacaacgg
     5161 cggccgcctg gcggacttcg tctttcagtg cacgggcgta ggcaaggccg gggccaacgc
     5221 ctggaagttc gtgaagcgcg gcggcggcct gtgcgaagtg ggctttttca tggatggagg
     5281 ggaaagcgtt atcaaccacc attacgacct ctgcaacaag gaggtgaccg ctgtaggctc
     5341 ctgggtgtac tccccgcagg actacccaac tacattcgac tttctgaagc gagcctacgg
     5401 catcggcctg ccgctgacca agctgatctc ccaccgcttc aagctggatg aaatcgcgga
     5461 agccctggaa accaacgtcc agatgaaagg catcaagatt gccgtcatct gcggctagcc
     5521 ggaaaactct tcatttcttc ccatgccgcc gtttttcaaa aacggcggct tttttacgcc
     5581 atctttcccc gtaaaaaaca ctcagcctcc caaagattaa tcatcatccc aatccagaaa
     5641 acggcccgta ttgggaggac aggccatagc ggaaacgatt ttttccttca accgctccag
     5701 tgcatcaggg tcaagaacca tgggttcgct cgctttaagg cacaaacctt tccggtacac
     5761 ctgcctttcc aacgtcagat atttattgac gatgccattg gaattcaggg cggcgtcaac
     5821 gacgaaatgc agcagataat cagcatctcc aaggtagaaa tcaaactcat aaatccagga
     5881 aaaacagtat tcttcgtcct cttcgcaaat catttccctg gcaggaattc cgcaatcata
     5941 cagatgattg agttccctca acagggtcag aatatgctca cgcctccgca cggttacctg
     6001 gcatcccagc tccccaacca tgccctgaag gcagtcgatg accaaatcat cacactgttc
     6061 ttccatgtca aggagaactt cccggacctg cgcccactgc cgctccaatg gttcctcgga
     6121 atacataaac cgggcaaagg gggaaggttc acctgcccca gcgttccgta atgccgccgt
     6181 aggagtcaat gcggcggtca cggaaaaagg gccagatgcg gcggtggtct tccaatgcgt
     6241 ccaggtcaag gtccgcgtac aggatggctt catcgctgac gggagccttc gccacaatct
     6301 ggccatagta gtccgccaca aaggactgcc cccagaattc gctttcccct tccgtcccca
     6361 cccggttgac ggcgcagaca tagcagccgt tggctacggc atggccgcgc tgtacggttt
     6421 cccacgcgca gtgctgctgt tcccccagca ggggcttttc ctccggcagc cagccgatgg
     6481 ccgtggggta aaaaatgatt tccgccccct gcatggccgt caggcgcgcg gcttccgggt
     6541 accactggtc ccagcagatg agcacgccga tgcggccgaa tttggtgtcg aacgccttat
     6601 agcccaggtc gccgggggtg aagtagaatt tttcttcaaa cccgggatcc tgcggaatat
     6661 gcatcttgcg gtacatgccc aggaacgaac cgtccgcatc caccacccag gcggtattgt
     6721 ggtaaagccc ggtggcgcgc ttttcaaagc cggaggcaac aatcaccacg cccagttccg
     6781 ccgccagttt cctgtgggct tccgtcaatt ccccgggaac aggttccgcc aggtcgaata
     6841 aatcgcattc ctccgtcctg caaaagtatt ccgtggtaaa aagttcctgg gtgcagacaa
     6901 tctgcgcgcc gttggcggcg gcttcacgga tggcggattc cgtcttttcc ttgttcacgg
     6961 aaggccgggc atctacggac agttgaatga gtgcaatttt cggcatgccc cttccttacc
     7021 ccgtttccct tcccattcca agcagggaaa atgaacgtgg cgcgattatt tggtggaaaa
     7081 cggcccggaa agtgctatga aattgcaata tggaacccgt cacccccact ctgcccgacg
     7141 acgacgtagc cgccattgac cggctcggcg ccatttacaa ggagctttcc tcggaattga
     7201 gcaaaatgat cattggccag cagcgcgtcg tggagctgct gagcatcgcc ctgttttccc
     7261 gcgggcatgc cctcctgatg ggagttccgg gcctggcgaa gacgctgctg gtcagttccg
     7321 tggcaaaaac gctggacctt agtttcaacc gcatccagtt cacgccggac ctgatgcccg
     7381 cggacattac cggcacggac atcatccagg atgattccgc cggcggcaag cgccagttcg
     7441 agttcatcaa ggggcccgtg ttcgccaaca tcgtgctggc ggacgaaatc aaccgcgccc
     7501 ccgccaaaac ccagtccgcc ctgctggagg ccatgcagga gcgccgcgtc accgtcatgg
     7561 gccgcacctt caccctgctc ctcccctttt tcgtgcttgc cacccaaaac cccattgaac
     7621 aggaaggcac ctaccccctg ccggaagccc agctggaccg cttcatgttc ctgattgaag
     7681 tggactaccc cacggaggag gaggaaatgc gcatcgcccg ggaaaccacc ggaacccgga
     7741 gcgaggaact caagcacatc ctctccgggg aggaaatcat cttttaccag gatctggtgc
     7801 gccgcgttcc cgttccggaa catatttacg agtatgccgt gaaactggtg cgcgccaccc
     7861 gccccaccct ggaaacttcc ccggaatggg taaagcagta tgtagcctgg ggcgcaggcc
     7921 cccgcgccgt ccagttcctg attcttgggg ccaagacccg cgccgctctc cagggcagct
     7981 acatcgtccg cagggaagac atagacgcca ttgtggaacc tgtgctgatg caccgcgtgg
     8041 tcaccaactt cgccgcggaa tcccagggca tcacgccccg taaggccgtg gcgcgcctgg
     8101 cggcgaaaat cttaccgccg tcccggcatc ttcaatcccc t
//
LOCUS       DAOMSU010000081         8069 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_667_length_8069_cov_2.458073, whole genome shotgun sequence.
ACCESSION   DAOMSU010000081 DAOMSU010000000
VERSION     DAOMSU010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8069
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_667_length_8069_cov_2.458073"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            675..1415
                     /locus_tag="PK077_04960"
     CDS             675..1415
                     /locus_tag="PK077_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRN23682.1"
                     /translation="MAFSAPSIPPSALPATLKPEPHQRDPYDWNARHEAVKKRNRTVK
                     PEYVMIGDSITHHWGGEPSGDGKSTGAGSWEKLFRPHTATNMGFGFDYVDNAYYRVQN
                     GELDGISPRVIIVLLGTNNLGHRKDTPQACADNIRAFVRLVRRKCPSSKILLLGVLPR
                     KEKNLAEPVKQTNRLLAELHNGKNIFFADPGKALLSADGASPQNEFMKDVVHPNARGY
                     EVLEKELAVLLKKLDAKYRGGKQAGKHS"
     gene            1500..3179
                     /locus_tag="PK077_04965"
     CDS             1500..3179
                     /locus_tag="PK077_04965"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRN23683.1"
                     /translation="MKNLLFALLTGSFCCCYAQQKAAPVPEPEVVATPPADAGRGLIR
                     VDSREIRHYSGTRKGPDYLVSRDNGKTWEMKAAPAGYPPNYGGIPKESPAIVRNPLTR
                     EFIRVQPIGGFVFLSKGGLDGKWFAVTNDGKLEEDWKDPEKRKNLKKLGGIMRTPVFV
                     NKGRRVIVPFHNMGSGTKFHISDDGGLTWHVSRNGVTSPKHEARPPHQGVRWFNNAVE
                     ATVLEMKDGSLWALVRTSLDQAWQAFSRDYGETWSKPEPSRFFGTLTMNTLGRLDDGT
                     IVSLWTNTMALPENATAGNGTWEDVFTNRDSHHIAMSGDEGKTWYGFREIILDEHRNH
                     PGYATLDGPEDRGKHQSEMVQLDKNRILISLGQHKNHRRLVIVDRRWVGAKTRATQTG
                     KDSDSQWTIHTYIPQKKGHCSYNRKPSAELVPDPSGSTKKVLQIKRLNDPELVNEKSN
                     VDYRNAGAAWNFPNGTTGLVKFRFRAADGDQADDSGLQVSLTDRLFNACDSTTKDYAL
                     FTFPIRLRPAPHLLLGMKKVPFTPGAWHEISLLWQGGGRLWCLWTERRPER"
     gene            complement(3300..4652)
                     /locus_tag="PK077_04970"
     CDS             complement(3300..4652)
                     /locus_tag="PK077_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23684.1"
                     /translation="MTLRKKTLWFLGALSFFASLAATFAVFWLNRNDSSPAEWNFNPI
                     WTKAEKEQILTVANFTAHRSPERFIYRGLSLNSRHIDWHMFSEVSMRQYMKDEIRERL
                     QMIRDTAESADGRCITPSHETLLHLAAGLGEVQLLNRLLQRHADPNVQIILPQQAFPS
                     IPDSEQGNTPLTYACQPGMDDSTPLPVHNRISCLNLLIQYGADVNKPGPLGWPPLVIT
                     CVAGIGGAPYEEAALFLLKCGADISLQPELKGKKTSLLTWATAAGYPLLVRKLCEAGY
                     DPNFRGEMVPPLLSLNLNTPNTALQIAHILLEYGADVNAAMPASTTLPETTGDTALLN
                     LCRQLAFIDVSQLPQIRELVSLFISEGADVNHQNAAGETPLMACCRGMLLGDDSLDRL
                     KLGIARLLLDHRADPSLRDKYGRTALQRIGNRSNEHLQMVLKYLPELSAPPLPKKENR
                     "
     gene            5199..6038
                     /locus_tag="PK077_04975"
     CDS             5199..6038
                     /locus_tag="PK077_04975"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HRN23685.1"
                     /translation="MDSVKLIASDMDGTLLNGKGELDPAFFPLFRELELRGIRFAAAS
                     GRQYDGLLRTFAPVADRMLFIAENGAYAAAGRKEWLLLDMDHETVAGLIAGIRCIEGT
                     CIVLAGRDSAYIEDKQPEFVREARKYYTRCQEVGDLLDVRGDKLLKIAVYDFLGAGEN
                     SYPRLKHLEHGFQVAVSGEKWMDISRKGANKGAALELIQRKLGISSEETMVFGDQMND
                     ASMIRQARFSYAMANAVPEIRSMAAFEAPSNEENGVMQVLGKMLDAHSPQGRAGNCRK
                     PRL"
     gene            6656..>6985
                     /locus_tag="PK077_04980"
     CDS             6656..>6985
                     /locus_tag="PK077_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRN23686.1"
                     /translation="MNQEFFSVLVQVAAALGLAGMILLLSILMGKRSSVRKPADIPYE
                     CGMIPMGEGAPMFSVKFYMVAMLFVIFDLEVVFLYPWAVNFRDYIMMNPESFTAMCIF
                     IGILLVAY"
     assembly_gap    6986..7001
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7002..7256)
                     /locus_tag="PK077_04985"
     CDS             complement(<7002..7256)
                     /locus_tag="PK077_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23687.1"
                     /translation="MMNFLIAQTLSLFHSSGKRNHLLYNMILHANRHPEKGQDLRIFL
                     PRHFPRQFFFHIKNAVFLMEHGVGKREQKRSTGIVVPVQGT"
     gene            7261..>8069
                     /locus_tag="PK077_04990"
     CDS             7261..>8069
                     /locus_tag="PK077_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN23688.1"
                     /translation="MPLPKTTQGVYRLSVSTFYFLQGLVFASWASRIPDIKSALGLND
                     ADLGSVLFAVPVGQMSAMALSGYLVGRCGSRKILMAASVFYPAVLVCLGMAGSFWELA
                     AGLFFFGVAANLTNISVNTQGVGVERLYRCSIMARFHGLWSLAGFFGALLGAAMVDWH
                     ISAETHFIAIFLICMIILAAFSPYLLPRDARRSSTQGGGMFRSMDAYVLVIGLIAFGS
                     MVSEGTMFDWSGVYFESVIKPGPGLVQMGYVAFMSTMALGRFTADRLVMRFG"
BASE COUNT         1786 a         1892 c         2372 g         2003 t
ORIGIN      
        1 agaggggagg caattgtatt cgtctttggt gagttatgtg cagtccagag attttaaccc
       61 gaagatgata tgttccccgg aagagttcaa ggcgttgttc tcaggcaata ccctttcgcc
      121 gaatatcaaa aatgtcgaca acgtaacgga ttacaaataa ttggatctct tttcctttta
      181 cttgccgaat ttctattgca gtatcttagc gattaccgga aacctcatgt atgcttttgt
      241 ttattgctgg cgggagtttt ttgtgtcgtc tttttctccg gtgcctgtgc tggagaactg
      301 cttcctatgc tgaagcccca ggagaaccag cgggccgtgc atgactggaa aaacgccatg
      361 aggcagtgat ggaacggacc cggaccgtca aacgggggta tgtcgttttt ggtgaaagca
      421 ttaccctccg ttggggagtc gagctttcct ggggcaacag ggctctcgga acaggaaagg
      481 ccggatgcgg tgttcgcctg gccgctgttg cggcgggatt tgccggacgg gcagtggtct
      541 tttttactct gccggcaaaa gtggcgcctg cgatcttgat gcacggcagg atttgcttaa
      601 actggaaacc ggtttcactt ttttatcatg aatttcttct cctacatgct cgccatattg
      661 ctgggggggc cagtgtggcg ttttccgcac cttccatccc tccctccgcg ttgcccgcaa
      721 ccctgaagcc ggagccccat cagcgggatc cgtatgattg gaatgcccgg catgaagccg
      781 ttaaaaaaag aaaccggacc gtcaagcctg aatatgtgat gataggggac agcattaccc
      841 accactgggg aggggagcct tccggggacg gaaaaagcac gggagcgggt tcctgggaga
      901 aattattccg tccgcatact gcgacgaata tgggctttgg atttgattat gtggataatg
      961 cctattaccg tgtgcaaaac ggggagctgg acggcatttc tccgcgggtc atcatcgtgc
     1021 tgctgggaac caataatctg gggcaccgca aggatacgcc gcaggcatgt gcggacaata
     1081 tcagggcgtt tgtccgcctg gtgcgccgga aatgcccctc ttccaaaatt ctgttgctgg
     1141 gcgttcttcc aaggaaggag aaaaacctgg cggaaccggt gaagcagacg aacaggctgc
     1201 tggcggagct gcataacgga aagaacattt tctttgccga tccgggaaag gctcttcttt
     1261 ccgcagacgg tgcttctcct cagaatgaat ttatgaagga tgtggtacat cccaatgcca
     1321 ggggatatga ggttttggaa aaggaactgg ccgtactttt gaaaaagctt gatgcaaaat
     1381 accggggagg gaagcaggcg ggaaagcatt cctgatgatc gtcttctgat ggaaggcagg
     1441 attttgcttg aagggaaagc cccggctgag gaaaatatac ggcatttgat atttagttta
     1501 tgaaaaacct gttgtttgca ttgttgaccg gttccttttg ttgctgttat gcccaacaga
     1561 aggcagcacc cgttccggag cctgaagttg tcgccactcc gccggctgat gcggggcgcg
     1621 gcctcatccg tgtggactcc cgtgaaatac gccattattc cggtacccgc aagggaccgg
     1681 attacctggt cagcagggat aacggaaaaa catgggaaat gaaggccgct ccggctggct
     1741 accctcccaa ctacggcggc attcccaagg aatctccggc tattgtgcgc aaccccctga
     1801 ccagggaatt cattcgtgtg cagcctatcg gaggctttgt atttctttcc aagggggggc
     1861 tggacggcaa gtggtttgcc gtcacgaatg acggcaagct ggaagaagac tggaaagatc
     1921 cggaaaagag gaaaaatctg aaaaaactgg gcggcatcat gcgcacccct gtttttgtga
     1981 acaagggccg cagggtgatt gttccgttcc acaacatggg cagcggtacc aagtttcata
     2041 tttccgatga cgggggactg acctggcatg tatccaggaa cggtgttact tcccccaaac
     2101 atgaagccag gcccccccac cagggtgtca gatggttcaa caatgccgtg gaagccacgg
     2161 ttttggagat gaaggacggt tcgttgtggg cgcttgtccg tacttccctg gaccaggcgt
     2221 ggcaggcttt ctccagggat tacggtgaaa catggagcaa accggaacct tcccgctttt
     2281 tcggcaccct gaccatgaat acgttgggcc gcctggatga cggaaccatc gtttccctgt
     2341 ggacgaatac gatggctctg cctgaaaacg ccacagccgg caacggaacg tgggaagacg
     2401 tgttcacgaa ccgtgattcc caccacattg ccatgtccgg ggacgagggt aaaacctggt
     2461 acggattccg ggagattatc ctggacgaac accgcaacca tcccggctat gctacgctgg
     2521 atggtccgga agaccgcggc aaacaccaga gcgaaatggt gcagctggac aaaaaccgta
     2581 tccttatttc cctggggcag cataaaaacc accgccgcct ggttattgtg gaccgccgct
     2641 gggtaggggc caagacgcgt gctacgcaga cggggaaaga ttcggattcc cagtggacca
     2701 ttcatactta tattccccag aaaaagggcc attgcagtta taaccgcaag ccttccgcag
     2761 agctggttcc ggatccgtcc ggaagtacga aaaaggtgct gcaaatcaag cgtctgaatg
     2821 atcccgaact ggtcaatgaa aaatccaatg tggattaccg gaacgctgga gctgcctgga
     2881 actttccgaa cgggaccaca gggctggtca aattccgctt ccgtgcagcg gacggggatc
     2941 aggcggatga ttccggcctt caggtctctc tgacggatcg gcttttcaat gcctgtgatt
     3001 ccactacgaa ggattatgcc ctgtttacct tcccgatcag gctgagacct gcgccccatc
     3061 tgctgctggg gatgaaaaaa gtgcctttca cgcccggcgc gtggcatgaa atttcccttc
     3121 tgtggcaggg ggggggcagg ctgtggtgtc tttggacgga aagaaggccg gaacgttgaa
     3181 aatggctaat aagtccccca atggagccag ttatattcat ttcatcagca ccggttccca
     3241 accggatgcc ggcattctgc tggatacggt gaatgcccgt gtgaaataaa cggcggtatt
     3301 caacggtttt ccttcttcgg aagcggcgga gcgctcaatt ccggcaggta tttcagcacc
     3361 atttggagat gttcgtttga ccggttgcct atccgctgga gggctgtgcg tccgtatttg
     3421 tctctcaggg aggggtctgc gcggtggtcc agcagaagac gcgcaatacc cagtttcagc
     3481 cggtccaggg aatcgtctcc cagcaacatt cctcggcagc acgccatgag gggagtctct
     3541 ccggcggcgt tttgatggtt gacgtccgct ccctcgctga tgaagagact gaccagctcc
     3601 ctgatttgag ggagctggga cacatcaatg aaagccagct ggcggcacag gttcagcagg
     3661 gccgtgtccc ccgtggtttc cggcagggtt gtggacgcgg gcatggccgc attcacgtcc
     3721 gcaccgtatt ccagcagaat atgtgcgatt tgcagtgcgg tgttcggagt gttcaggttc
     3781 agggaaagaa ggggcgggac catttccccg cggaaattgg gatcgtaccc ggcttcgcaa
     3841 agcttgcgga ccagcagggg gtatccggcg gccgtggccc atgtgagcag acttgttttt
     3901 ttacctttca attcaggctg gagggaaata tccgcaccgc atttcagaag aaacagggct
     3961 gcttcctcat agggtgctcc tccaataccg gccacacagg tgatgacgag gggcggccag
     4021 cccagcggcc ccggtttatt cacatccgca ccgtattgaa tcaggagatt aaggcaggag
     4081 atcctgttat gcaccggcag gggcgtggag tcgtccatgc ctggctggca ggcgtaagtc
     4141 aggggggtat ttccctgttc ggagtccgga atggagggaa aggcctgttg cggtaggatg
     4201 atctggacgt tcgggtccgc atggcgctgt aaaaggcggt tcagcaactg cacttctccg
     4261 agcccggcgg caagatgcag cagggtttca tgggatggag tgatgcagcg tccgtctgcg
     4321 ctttccgccg tgtcccgtat catctgaagc cgttccctga tttcgtcctt catatattgg
     4381 cgcatggaaa cttcggaaaa catgtgccag tcaatatggc gtgaattgag ggaaagcccg
     4441 cggtaaatga aacgttccgg gcttcgatgg gctgtgaaat tcgctacggt aagaatttgt
     4501 tctttttccg cttttgtcca gatagggttg aaattccatt ccgcgggaga agaatcattc
     4561 ctgttcagcc agaaaacggc gaaagtcgcg gcaagagagg caaaaaagct caaagctcca
     4621 agaaaccaga gagttttctt tcttaaagtc atgtgtatca taagcagggg tactgcgaaa
     4681 ggccatgatg caggaatcca taactccggg caatgaaaaa atagtatgag caaagaatgc
     4741 ccggggcaaa ccatgtggga attcgggtgg cggattgcaa attcggagga gaattttttc
     4801 ctcttcatct gggaaaaaag ctcgtagata aagagaatta cgaaggaaac ggaaaatatg
     4861 gtgtcatggg ccggacttca caaaaattat ggagtttatg gcgtggatca tttaactcca
     4921 attatttgaa ttgtatccga atttgcaatt cggtgtgata cgctaaatat tgcggatgtg
     4981 cggatggttt caaattattt gcatttgccg ctgctgcact cacggtagaa tgaaggaagc
     5041 tgaaacttat ggaagaaatg gagattctcc cgtaaaatga tggagagaac tgtatccttg
     5101 ctgctggcct cctggtcttt cctttcgctg tccgctccat gctgtctgaa gccgctcttg
     5161 ccctggaagt aaggataaac gatgattctg gcagcgttat ggattccgtc aaacttattg
     5221 cgtccgatat ggatgggacg ctgcttaatg gaaaaggtga attggaccct gcgttttttc
     5281 ccttgtttag ggaacttgag cttcgcggca ttcgttttgc ggcagcaagc ggccgccagt
     5341 atgacggatt gctccggacc tttgcccccg tggcggaccg gatgctgttt attgccgaga
     5401 atggcgctta tgcggcggcg ggtagaaaag aatggctgct tctggacatg gatcatgaaa
     5461 cggtagccgg tttgattgcc gggatcaggt gcattgaagg aacctgcatc gtcctcgcag
     5521 ggcgggattc cgcatatatt gaggacaaac agccggaatt cgtccgggag gcccgcaaat
     5581 attacacacg ctgtcaagaa gtgggggacc tgctggacgt gcggggagac aaacttttga
     5641 aaatagcagt atatgacttc ctgggagccg gggaaaacag ttatccccgg ctgaagcatt
     5701 tggaacatgg cttccaggtg gctgtctccg gagagaaatg gatggatatc agccggaaag
     5761 gggccaataa gggggccgct ctggaattga ttcagaggaa actgggaatt tcttcggagg
     5821 aaacgatggt tttcggagat cagatgaatg acgcttcaat gatacggcag gcgcgtttca
     5881 gttatgccat ggcgaatgcc gtgccggaaa tccgttccat ggccgcattt gaggcgccgt
     5941 ccaatgagga aaacggagtg atgcaggtgc ttggaaagat gttggacgcc cattctccgc
     6001 agggaagggc cgggaattgc aggaaacccc gtctctaatt gccgggtaat ggaataaaat
     6061 cagggttatg ttgatgttat tgtcagattt ttttaatatg ctggcgatgg ccgtgttcgc
     6121 ttgcattggc ggtgtttttc tgggaatggg ctttctgctg cttaaattgg cgggaatttt
     6181 tttcggaata ttggcggggt gtattattct gatggaagcg gccggaaggt ggatttccca
     6241 tttgatgggg gcgtgagtcc gttattcccg gcaatgaagg ggattcctgt acgctttccg
     6301 tgaggggaga gctttctgat ggctgggcca ttgccggctg ctgttgttgc cggttcattg
     6361 ccgaatgctg aagtggaagg ctgcatcctg cctggattct gcgtgttaaa accggatagc
     6421 ggagttttgt gataaacaga cgtttgccgt aagatgccct ggaagacaga gccccgcaga
     6481 aaggaagtcc caaggatatg aaatgccggg cgtccccatg gaagggactg ttgcctttgt
     6541 actatttgac agggcgggag acggaatttt accgtccctc ccgtcagcat gaaagctgtt
     6601 gtccttttta gttttgcttt tccgcaatgt ctgtggtaca cccgtgccag acgtcatgaa
     6661 tcaggaattt ttttcagtgc tcgtgcaggt ggcggccgcc ctggggctgg ccggcatgat
     6721 cttgttgctc agtatcttga tgggcaagcg ttcttccgtg cgtaaaccgg cggacattcc
     6781 ctacgaatgc gggatgattc ccatggggga aggtgccccc atgttctctg taaaattcta
     6841 tatggtggcc atgctctttg tcatttttga cttggaagtg gtattcctct acccctgggc
     6901 cgtcaatttc cgggactata ttatgatgaa tccggaatcg ttcacggcca tgtgcatttt
     6961 cattggcatt ttgctggtgg cctacnnnnn nnnnnnnnnn nggtgccctg aactggcacc
     7021 acgatccccg ttgaccgttt ttgttccctt ttaccaacgc cgtgttccat gaggaatacg
     7081 gcgtttttta tgtgaaagaa aaattgcctt ggaaaatggc gggggagaaa tatccgtaaa
     7141 tcctgtcctt tttccggatg cctgtttgcg tgtaaaatca tattgtacaa aagatgattt
     7201 ctcttgccag aagagtggaa tagggatagc gtttgcgcga tcaggaaatt catcatgaat
     7261 atgcctcttc ctaaaacgac gcaaggtgtt tatcgtctgt cggtcagtac cttttatttt
     7321 cttcagggtt tggtgttcgc cagttgggca tcccgcatcc cggacatcaa aagtgcattg
     7381 gggttgaacg atgccgactt ggggtccgtt ctctttgccg tcccggtggg gcagatgtcc
     7441 gccatggcgc tatccggtta cctggttggc cgttgcggca gccggaaaat actgatggcg
     7501 gcatccgtct tttatcctgc cgtgcttgta tgcctgggta tggcggggtc tttctgggaa
     7561 ctggcggccg ggttattttt ctttggggta gccgcaaatc tgacgaatat atccgtcaat
     7621 acgcagggag tgggagtgga acggctgtac cggtgcagca tcatggcccg ttttcacggt
     7681 ttatggagcc tggcgggttt tttcggagct ttgctgggag ctgccatggt ggactggcat
     7741 atttctgcgg aaacgcattt catcgccatt ttcctgatat gcatgattat tctggctgct
     7801 ttttcaccct atcttctgcc gagagatgcc cggcgttcct ccactcaggg cggcggcatg
     7861 ttccggagca tggatgccta tgtactggtc atcggactga tcgctttcgg aagcatggtg
     7921 agcgagggaa ccatgtttga ctggagcggc gtgtattttg aaagcgtgat aaaacccggc
     7981 ccggggctgg tgcagatggg gtatgtggca ttcatgagca ccatggccct ggggcgtttt
     8041 acggcagacc gcctggtgat gcgcttcgg
//
LOCUS       DAOMSU010000082         8029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_673_length_8029_cov_2.424128, whole genome shotgun sequence.
ACCESSION   DAOMSU010000082 DAOMSU010000000
VERSION     DAOMSU010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 8029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8029
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_673_length_8029_cov_2.424128"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="PK077_04995"
     CDS             <1..580
                     /locus_tag="PK077_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23689.1"
                     /translation="DGEDRGLALLSKYRIADNRSVADMPVSGEAKRKKTMLRGILDAT
                     VSMPDGRLFRLVGIHLKSRLSRDGSAEDTRRREAYALRDYLNEALASQDGMPLLLYGD
                     FNDGPSDSAVQVIQGPVKTEYRLNRLKPRDSRGETWTIYYEDGDTYHSFDHLFLNNTL
                     KKRLGRKPPMGILDSPPSLQASDHRGVWVELR"
     gene            888..1598
                     /locus_tag="PK077_05000"
     CDS             888..1598
                     /locus_tag="PK077_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRN23690.1"
                     /translation="MRYFSKHSGMAPRCSLSGSKGFTLVELIVVITIMVAMMALAASM
                     LRGGGRGQGLQAAVEMVDGMVQEARLDAMGKGTWSRLIIVSTPDDEARNMRTLGVMSK
                     NTRTGKWHLVNRLQTLPAGFYVSPTYSTLLEGAKKARGEKSTARDFASRDGQDTVNLP
                     GNRMTDIYFIEFDEEGRMSQPNAPTRLVVVAGSAGNGKEERPTPMVDGKPGLAGGIVI
                     YPKGNISRLRTTEQVIPN"
     gene            1646..2431
                     /locus_tag="PK077_05005"
     CDS             1646..2431
                     /locus_tag="PK077_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRN23691.1"
                     /translation="MKQSRAKGFTLTEVVLAIGVVAVLIVVFMAMFIPARRTVQSALT
                     IREADRIVHALTAELGELRNSERAASNARKSSSSKYVSAFDKAFYWMQFTSRPATTIL
                     VYNYRADLTKGARKDGTPQPWLEDGGSIPGKNTAVVTGVCLANNKERWDDFKALVGPV
                     FAVRMTQLVVERMDSSSYGYKLAPKYGTIYNPYNRGKVIKEPSLYVYTPEKGGGLNLP
                     WGAEVLYQAEFFQLLNTDPERLQHLTWENLKTPVFTRNLAFRR"
     gene            2462..3517
                     /locus_tag="PK077_05010"
     CDS             2462..3517
                     /locus_tag="PK077_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRN23692.1"
                     /translation="MKTFAKIKSGAFTLIELLAAMTITTVLVLVIVALTSRGVDIWRW
                     VLQDVRTTTLARTARDTMTKDFESMQFRPGNPFEWMLVQRDSDLVSRAEKTKVSRKKG
                     RSSSLNEARVNLMTMGPEGAKITNASQLVFFTTATDRNPAKSSMDMRNDRIIGDVNCV
                     GYKLLYRDQILDQDATAESDGFPVYSLYRNLISAEDTVRNLLGQQDLWSAYARYQQDE
                     TVPSNFLVENIVEMTLIFEVDYQKKIGNSGSNKSDKDASQRASVLVPVMTTGACTQMD
                     VYGNRLDIIGSSVNIDDLKSGRVTAVTISMTVVTDEGMALVDQIRQKKRPAPSPEEFF
                     ERYTRSFTQRIALPMNR"
     gene            3609..4349
                     /locus_tag="PK077_05015"
     CDS             3609..4349
                     /locus_tag="PK077_05015"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23693.1"
                     /translation="MLGFTNIVGNLSPLPELVLWRDPVARSGEEAMAVDEALLEWGRK
                     PVLRAYRWAEPTVTYGYFDEEATARAIFPGEELHFVRRWTGGGIVDHRQDIPFTLAMH
                     RKGEKERPSSAVLYRWIHGALARVLRDCGVECVMLDGDAPDGGRACFSSPVTSDLVLP
                     GGEKLAGGGQRRVRGGVLHQGSIQNCLLPSGWDELLAARLAEKHSLCVDAELFPGMNA
                     CVEELLASKYRTEAWRSGVRHGRPSQSR"
     gene            4365..5354
                     /locus_tag="PK077_05020"
     CDS             4365..5354
                     /locus_tag="PK077_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRN23694.1"
                     /translation="MNYLPDPGRYSHAAYRRCGRSGLLLPPVSLGLWHNFGVADDFEN
                     ARRLIHGAFDAGITYFDLANNYGPPPGSAEECFGRIFMQDLSRFRDELVIATKAGHLM
                     WSGPYGDWGSRKHMLASLDQSLSRMKLEYVDIFYAHRHDPETPLEETAGALITAVRSG
                     KALYAGISKFPAEQARQMCSMLGEAGVPCLVHQVRYNMFSREPETSVFQAVRETGIGC
                     VAFSPLAQGMLTDRYLEGIPEDSRAAGSSVFLTKERVLQHGDKIRRLGALARSRGQSL
                     AQMALAWVLKDPVVTTALIGASRPSQIRDCLKALDSAPFTPEELSFIDANADR"
     gene            5486..7714
                     /locus_tag="PK077_05025"
     CDS             5486..7714
                     /locus_tag="PK077_05025"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRN23695.1"
                     /translation="MITIQDLFWLIPSASVLALIFAGIFFYRMKAQDEGNEVMKMIAR
                     HVRSGAMAYLRQQYKIVAIFFVLITVVFAFLAYCLHIQNPWVPFAFISGGFFSGLAGY
                     IGMKTATYASGRVANACRHSLDAGLQIAFRSGAVLGLTVVGLAMLDIGAWYWVLNWFY
                     GDMELSMRLVVITTTMLTFGMGASLQALFARVGGGIFTKAADVGADLVGKVEAGIPED
                     DPRNPATIADNVGDNVGDVAGMGADLYESYCGSILASAALGAAAYMAVPEMAIKAVLT
                     PMLIAALGTILSLLGVYAVRVKRGASQTELMAALNRGINLSSFLIAIASAFLLQLIGL
                     DNWAGIWGAIVTGLAVGIVIGKSTEHYTSHDSYPCRKIAHSAKTGPATVIISGIGIGM
                     ISTCIPVITVVVGTVLAYGMASGDWHFTGAEMSKGLYGIGIAAVGMLSTLGLTLATDA
                     YGPISDNAGGNAEMSKLDPEVRRRTDALDALGNTTAATGKGFAIGSAALTALALLASY
                     IEELKIAILHWSEATGNTIYKINDAVSVEVSKIATASLSDFMGYFQVTLMNPKVLMGL
                     FVGAMMSFMFCGLTMNAVGRAAEKMVKEVRRQFKEIKGILTGEAEPDYVRCVEISTAG
                     AQHEMIWPSVLAVITPICMGIVFGVPGVFGLLAGGLASGFVLAVFMANSGGAWDNAKK
                     YIEQGHVGGKGSESHKAAVIGDTVGDPFKDTSGPSLNILIKLMSMVAIVTAGVNIAVT
                     LF"
BASE COUNT         1618 a         2138 c         2360 g         1913 t
ORIGIN      
        1 ggatggggaa gaccgtggtt tggcccttct ttccaaatac cgcatcgcgg ataaccgttc
       61 cgtagcggac atgcctgtat ccggagaggc gaaacggaaa aagacgatgc tgcggggcat
      121 tctggacgcc acggtcagca tgccggacgg acggctgttc cgcctggtgg gcattcattt
      181 gaaatcgcgc ctcagccgtg acggttccgc agaggatact cggagaaggg aagcctacgc
      241 cctgcgggac tacctgaatg aagctcttgc ctctcaggac ggcatgcctc tgcttctgta
      301 cggagatttt aatgatggcc cgtcagacag cgccgtgcag gtcatccagg gaccggttaa
      361 aacggaatac cgcctgaatc gtttgaagcc cagggattcc cgcggggaga cctggaccat
      421 ttactatgag gacggagaca cctaccattc ctttgaccat cttttcctga acaatacgct
      481 gaaaaagcgc ctcggccgca agcctcccat gggtatcctt gactctcccc cctcactcca
      541 ggccagcgac caccgcggcg tgtgggtgga attgagataa gcagggcctg atgttttttc
      601 agcaggggag atggaacgga aaaaatgaag gctttggaaa aggaggacaa aaaattcttt
      661 tcttgtcgtt ccggccttat ccttcttgaa atgcgggagt tggaccgacc gcctgttttt
      721 cttttccgga aggatggcgg aagcagggat ttccgcatgg cgattccttg ccggaatttt
      781 gttggacagg aacatatcag gcattcccgt tcctcaaaaa atgattgccg tgtccgtctg
      841 aaacgggtag catgccaaaa atcgttgttc aatcctgcat catctgtatg agatattttt
      901 ccaagcattc cggaatggct ccgcgctgtt ccttgtcagg gagcaaaggg tttaccctgg
      961 tggaactgat tgtcgttatc accattatgg tggccatgat ggccttggcc gccagcatgt
     1021 taagaggcgg aggcagaggg cagggccttc aggccgccgt tgaaatggtg gacggcatgg
     1081 tgcaggaggc gcggctggat gccatgggca aaggaacgtg gagccgcctg attattgtga
     1141 gcactcctga tgacgaagcc cgcaatatgc gcactttggg cgtgatgtcc aaaaataccc
     1201 gcaccgggaa atggcatttg gtgaaccgtt tgcagactct ccctgccggt ttttacgtca
     1261 gtccgaccta cagcaccctt ctggaaggcg cgaagaaagc caggggcgag aaatccacgg
     1321 cccgcgattt tgccagtcgt gacgggcagg ataccgtcaa ccttcccggc aacagaatga
     1381 cggatattta cttcattgaa tttgacgagg aaggccgcat gtcccagccg aacgccccca
     1441 cccgcctggt ggtggttgcc ggttccgccg ggaacggcaa ggaggagagg ccaaccccga
     1501 tggtggacgg caaaccgggc ctggcaggcg gcattgtgat ttatcccaaa ggcaatatca
     1561 gccgtctgag gacgacggag caggtgattc ccaattagtt tctgtgatat aataccggat
     1621 ttcccatttt atgttgacca agtgtatgaa acagtctcgt gccaaggggt tcaccctgac
     1681 ggaagtggtg cttgcgatcg gcgtggtggc cgtgctgatc gtcgtgttca tggccatgtt
     1741 tattcctgcc agaaggacgg ttcagtccgc cctgactatt cgtgaagcgg accgcatcgt
     1801 ccacgccctg acggcggaac tgggagaact ccgcaattcc gagcgtgccg cgtccaacgc
     1861 cagaaagtct tcctcctcca aatatgtttc tgcttttgac aaggcgtttt actggatgca
     1921 gttcacgtcc aggcccgcta cgaccattct tgtttacaat tacagggcgg atttgaccaa
     1981 gggggcccgc aaagacggaa ctccccagcc ctggctggag gatggcggaa gcattcccgg
     2041 caaaaatact gcggtggtga caggggtctg cctggcgaac aacaaagagc gttgggatga
     2101 tttcaaggcc cttgtcggtc ccgtcttcgc cgtgcgcatg acccagctgg tagtggaacg
     2161 tatggattca agctcctacg ggtataagct ggctcccaag tacggaacga tttataatcc
     2221 ttacaaccgc ggcaaggtga ttaaggaacc ttccctgtat gtttatacgc cggagaaagg
     2281 cggcggcctg aatttgccgt ggggggcgga agtcctgtac caggcggagt tcttccagct
     2341 gttgaatacg gaccctgagc gtttacaaca tctgacgtgg gaaaatttaa agactcccgt
     2401 gtttacgcgc aatctcgctt tccgccgtta gcgggcatta tctgaagacc tctgaaaaaa
     2461 catgaagaca tttgccaaaa tcaaatccgg cgcgttcacc ctgattgagc tcctggcagc
     2521 catgaccatc actaccgtgc tggttctggt cattgtggcc ctgacttccc gcggggtaga
     2581 tatctggaga tgggtgctgc aggatgtgcg gaccacgacg cttgcccgca cggccaggga
     2641 taccatgacc aaggattttg aaagcatgca gttccgtccc gggaatccct ttgagtggat
     2701 gctggtgcag cgggacagcg atttggtttc ccgtgcggaa aagaccaagg tttccaggaa
     2761 aaagggccgc tcttcctccc tgaatgaggc ccgcgtgaac ctgatgacca tggggccgga
     2821 aggcgccaaa atcacaaatg cctcccaatt ggttttcttt accacggcta cggaccgcaa
     2881 tccggccaag tccagcatgg acatgcggaa tgaccgcatc attggtgatg tgaattgcgt
     2941 gggatacaag cttctttacc gtgaccagat tctggaccag gacgctacgg cggagtctga
     3001 cggttttccc gtttattccc tgtaccgcaa cttaatcagc gcggaggata ccgtgcggaa
     3061 ccttctgggc cagcaggatc tatggtccgc ttatgcacgg taccagcagg atgaaaccgt
     3121 cccttccaac ttcctggtgg agaatattgt ggaaatgacg ctgatttttg aagtggatta
     3181 ccagaagaag atcggcaatt ccggcagcaa caaatcggac aaggatgcct cgcaaagagc
     3241 atccgtgctg gttccggtca tgactacggg cgcctgcacc cagatggatg tgtatggcaa
     3301 tcgcctggac atcataggca gcagcgtgaa tattgatgat ttgaaatccg gccgcgttac
     3361 tgccgtgaca atttccatga cggtggtgac ggatgaaggc atggctcttg tggaccagat
     3421 ccggcagaaa aagcgtcccg ctccctctcc tgaggaattt ttcgaacgtt atacccggtc
     3481 tttcacccag aggatcgcat tgccgatgaa ccgttaggct tgtatccggg aaaccgggga
     3541 gggcggaatc ggccgctgtt gctttttgtc tggagcgttc ctgccgttaa ttgttctgta
     3601 gttgccatat gttgggtttc acaaatatcg tcggcaattt atcccctctg ccggaattag
     3661 tgctgtggcg cgatcctgtc gccagaagcg gggaggaagc catggccgtg gacgaagctc
     3721 tgctggaatg gggcaggaag cctgtgctga gggcgtaccg ctgggcggaa ccgaccgtga
     3781 cttacggtta ttttgatgag gaggccacgg cgcgcgccat tttcccaggt gaggagcttc
     3841 attttgtccg ccgctggaca ggggggggga ttgtagacca tcggcaggat attcccttta
     3901 ctctggccat gcaccgcaag ggagaaaaag agcgtccttc cagcgcggtt ttgtaccgtt
     3961 ggattcatgg ggcgctggcc cgcgtcctga gggattgcgg ggtggagtgc gtgatgctgg
     4021 acggggatgc cccggacgga gggcgggcct gtttttccag ccccgtaacg agtgatcttg
     4081 tccttcccgg cggggaaaaa ctggctggcg gcggccagag aagagtccgc ggaggcgttc
     4141 ttcaccaggg gagcatccag aattgtcttc tcccttccgg atgggatgaa ttgctggctg
     4201 cccgcctggc ggaaaagcat tccctgtgcg tggacgcgga attgttcccc ggcatgaatg
     4261 cctgcgtgga ggaactgctg gcttccaaat accgtacgga agcatggcgt tccggcgtac
     4321 ggcatgggcg tccgtctcaa tcacggtaat ttcccttttt cccgatgaac tatcttccag
     4381 atcccggccg ctacagccac gcagcctacc gccgttgcgg gcgcagcggg ctgctgcttc
     4441 ctcccgtttc cctgggtctc tggcacaatt tcggcgtagc ggatgatttt gagaacgccc
     4501 gccggcttat tcacggcgcg tttgatgccg gtatcacgta ttttgacctg gcgaataatt
     4561 acggccctcc tcccggttcg gcggaagaat gctttggccg tattttcatg caggatttgt
     4621 cccgtttccg tgatgaactt gttattgcaa ccaaggcggg gcatttgatg tggtccggcc
     4681 cgtacgggga ctggggatcc cgcaaacaca tgcttgccag cctggaccag tccctttccc
     4741 gcatgaagct ggagtatgtg gatatttttt acgcgcaccg gcatgatccg gaaactcccc
     4801 ttgaagaaac ggcgggagct ctcattacgg cggttcgctc cggaaaggcc ctgtatgccg
     4861 gtatttccaa attcccggcg gaacaggcca ggcagatgtg ctccatgctc ggggaggccg
     4921 gcgttccgtg cctggtgcat caggttcgtt acaatatgtt tagtcgggaa ccggaaacat
     4981 ccgtttttca ggctgtccgg gaaacgggta tcgggtgcgt cgctttttct cccctggccc
     5041 aggggatgct gacggaccgt tatctggagg gtattcctga agattcccgc gcggcgggtt
     5101 cctccgtctt tttaacaaag gagcgggttc ttcagcatgg ggataagatt cgccgtctgg
     5161 gcgccctggc ccggagccgg ggccagagcc ttgcccagat ggccctggcc tgggtgctga
     5221 aagatcccgt agtgaccacg gctctgattg gagccagccg cccctcgcaa atcagggatt
     5281 gcctgaaagc tctggatagt gcgccgttta cgccggagga gctctctttt attgacgcaa
     5341 atgcggatag ataggcgctg cttttcccgt ggcgctggtc tttctgcagg aaggtgaatt
     5401 ttctcgttcc ggacacaggt tttttcttgg agtaaagtag agctggtgtt atgtttttac
     5461 cactttaatt taaagtgtac tgacaatgat aaccatccag gatttatttt ggctcatccc
     5521 atcggcttcc gtgctggccc tcatttttgc gggcatcttt ttttaccgca tgaaagcgca
     5581 ggatgagggg aacgaggtca tgaaaatgat cgcccggcat gtccgcagcg gcgccatggc
     5641 ctatctgcgg cagcagtata agattgtcgc cattttcttt gtgctcatta ctgttgtttt
     5701 cgcttttctg gcctactgcc tccacattca gaacccctgg gttcccttcg ctttcatttc
     5761 aggcggcttt ttctccggtc tggccggcta tatcggcatg aagacggcta cgtacgcttc
     5821 cggccgcgtg gcgaatgcct gccgccattc cctggatgcc gggctgcaaa tagctttccg
     5881 ctcgggcgcc gtgctcggcc tgaccgtcgt gggccttgcc atgcttgata tcggcgcctg
     5941 gtactgggtg ctgaattggt tttacgggga catggagctt tccatgcgcc tggtcgtcat
     6001 cactaccacc atgttgacgt tcggcatggg ggcttcccta caggcgctgt ttgcccgtgt
     6061 gggcggcggc attttcacca aggcggcgga tgtgggagcc gacctggtgg ggaaggtgga
     6121 ggccggcatt ccggaggacg atccccgcaa ccccgccacc attgcggata acgtgggcga
     6181 taacgttggg gacgtggccg gcatgggtgc ggacctttac gaatcttact gcggttccat
     6241 tctggcttcc gccgctttgg gcgcggctgc ttacatggcc gttcctgaaa tggcgatcaa
     6301 ggccgtcctt acgcccatgc tgatcgccgc cctcggcact attctttctc tgttgggcgt
     6361 gtacgcggtg cgcgtgaagc gcggcgcttc ccaaacggag ctgatggcag ccctgaaccg
     6421 cggcatcaac ttgagttcct tcctgatagc cattgcttcc gctttcctgc tccagctcat
     6481 cggcctggat aactgggccg gcatctgggg ggctatcgtc accggcctgg cggtgggcat
     6541 cgtcatcggc aagagcacgg agcattacac ctcccatgat tcctatccct gccgtaaaat
     6601 cgcccacagc gcgaagacgg ggccggccac cgtcattatt tccggtatcg gcatcggcat
     6661 gatttccacc tgcattcctg taattactgt cgtcgtgggc acagtgctgg cttatggcat
     6721 ggcttccggg gactggcatt tcaccggggc tgaaatgagc aaggggctgt atggcatcgg
     6781 cattgccgcc gtcggcatgc tttccacgct gggcctgaca ttggcgacgg acgcttacgg
     6841 ccccatttcc gacaacgccg ggggtaatgc ggagatgagc aaactggatc cggaagtgcg
     6901 ccgccgcacg gatgccctgg atgccctggg caacacgaca gccgccacgg gcaagggctt
     6961 cgccatcggt tccgccgctc tgacggcatt ggctcttctg gcctcctata ttgaagaatt
     7021 gaagattgcc atccttcact ggagtgaggc cacgggcaat accatttaca aaattaatga
     7081 cgccgtaagc gtggaggttt ccaaaatcgc cactgcttcc ctgtctgatt tcatgggcta
     7141 tttccaggta accctgatga acccgaaggt tctcatgggt ctcttcgtgg gggccatgat
     7201 gtccttcatg ttctgcgggc tgaccatgaa tgccgtcggc cgcgccgcgg aaaaaatggt
     7261 taaggaagtg cgccgtcagt ttaaggaaat caagggaatc ctgactgggg aggcagagcc
     7321 ggattacgtg cgctgcgtgg aaatttccac ggcgggagcg cagcatgaga tgatctggcc
     7381 gtccgtgctg gcggtcatca cccccatctg catgggcatc gtcttcggcg tccccggtgt
     7441 gttcggcctg ctggccggcg ggctggcttc cggctttgtg ctggccgtat tcatggcgaa
     7501 ctccggcggc gcgtgggaca atgccaagaa atacattgag cagggccatg tgggcggcaa
     7561 aggctcggaa agccataagg cagccgtcat cggcgatacg gtgggcgacc ccttcaagga
     7621 tacgtccggc ccgtccctga acatcctgat caagctcatg agcatggtgg ccatcgtcac
     7681 cgccggcgtg aatatagccg tgaccctgtt ctaaacagaa agattctttc acggaaaacg
     7741 gagggagtcc cgctgttcta gcgggactcc cttttttgaa tgaaatggat tttcatttag
     7801 agtctggtgt tccatagttg aattttccgc cgatgtctca aatgaatttc tgtaagactc
     7861 tgtcacgctt ggtaattcgg ggaaaaagag tatttttgac agatggaaaa ttgtttttcg
     7921 ttgacaagca agggtattat tctattctgt agtgaaaata taaaaagtca ggtaaagtat
     7981 gtttacttga tttttctctt ctctctcttt cttccggctt ctatcctgc
//
LOCUS       DAOMSU010000083         7970 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_687_length_7970_cov_2.570689, whole genome shotgun sequence.
ACCESSION   DAOMSU010000083 DAOMSU010000000
VERSION     DAOMSU010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7970)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7970
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_687_length_7970_cov_2.570689"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1091)
                     /locus_tag="PK077_05030"
     CDS             complement(36..1091)
                     /locus_tag="PK077_05030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN23696.1"
                     /translation="MTQSCPPQPQVSVIVPVYNQERYLEECISSIRRQTLAEWECLII
                     NDGSSDSSGEIARRFSEADSRIRYFEQKNGGVSSARNLGMRRASGRYLCFVDGDDFID
                     AAFLKHLLDASDRGASDLTVAGKLFCDRFPPDKIPALPTCGIFLRREFPLKNNLEFPE
                     GIHPCEDGLFSHFVLALTEKISFCPEAVYHYRQHEQGNHHQIRKRTADILPMIPRWLS
                     LIEEFYEQRHLWKRKAGHLVRFIEHEPFELRLLGMPFSPPEQEILYSIIRDFLTAHCT
                     AAECRRASLHLPFRLLLQSSGFSDFGRRLRRAGKNTGIRRKLLHFCPVPSWRRNGRAQ
                     LRQVREQLEEIRRNITF"
     gene            complement(1088..2296)
                     /locus_tag="PK077_05035"
     CDS             complement(1088..2296)
                     /locus_tag="PK077_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23697.1"
                     /translation="MKPMTHTQTVMLRLLTMCIPFRNLRKKWRTFLRDFLSDYDRDAR
                     RSMRAWRRHPVAGRTILILEPNYCHGEVIPGFCRHLLDMGYRVDVLMHHSLARQSPLC
                     CFKENDIHIFTAMCTSWKRLAKAPVLNRYEAILVSTSAFYDTPGWAGFLHMTGLDRFT
                     NLLAVEHELNDIPRFGEEGLERQGRLLTLGSFPKGTMVNPHFFGEIPPAPRNREPVFI
                     TVGSLSAQRKNHNLLIEALQTIYHEGHRDFSVIIVGEGELGEIPGHLRPFLHVAGKLD
                     FPAMYEAMRRADYFLPLLDPGNPAHDRYITTGITGSALLIYGFAKIPVIHEKFASFYG
                     FNDRNALLYGEETLGGAMLRAIRQTEEEYAEMQQALLQLGRDIDRESRSNLKRALAAC
                     SPSEPQQSRQ"
     gene            2694..3770
                     /locus_tag="PK077_05040"
     CDS             2694..3770
                     /locus_tag="PK077_05040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN23698.1"
                     /translation="MSVSVSIIVPCYNVAAYLDQCMESLAGQSMEDIEIICVNDGSSD
                     HTAEILREWRDRDGRVRVIDRKNSGVSAARNSGMEEAAGKYIGFVDPDDVVERNMFRR
                     LFDAAVEKDADVAVCGYHEFCDRGGMDMPESGWSPSAGFFPEEKAERFRRGTPWSRCA
                     GTVWNKLIRRKLLEENGVRFVPGLRQGEDLYFCLMLLTVAPRLLILPDRLYHYRRERP
                     GSASCGRDPRLGDFRMDLMGMESLARFWGERGLLDSPAAFGLVDYCMELMRRHFIMKE
                     GAFFKASPEDREVLLSGWKKWLELVGAENVLPSLDRWDRAFCRLLLDYPCRSNFFSAL
                     HSRLMSSRKGRRGAYYTLKRHLMK"
     gene            complement(3849..4412)
                     /locus_tag="PK077_05045"
     CDS             complement(3849..4412)
                     /locus_tag="PK077_05045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRN23699.1"
                     /translation="MKKVAILAAPGFEEIELMAPLDILRRLNMDVVLAGVQSDKVVST
                     HEVTVSTDTMLDKLHADKLDALILPGGAGSWVLRDTPEVIHLVKKMHEAGKLVAAICA
                     APIVLAKAGLVRDRNVTAYPAQDVYRELNEAGAHIVKDENVVLDGNMLTANGPGAAML
                     FGYSIGEYLGEDLQVAQLKEQMCYTGL"
     gene            complement(4476..5516)
                     /locus_tag="PK077_05050"
     CDS             complement(4476..5516)
                     /locus_tag="PK077_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HRN23700.1"
                     /translation="MAITIEMPRLSDSMHEGTVLRWFKKTGDFVKVGDHLADIETDKA
                     HVELQACEDGTLTEILVPEGGSAAAGAPIALLQPEFGAAVCGCPPRPSAICSPLAARL
                     AAEAGLNPATLRGTGPRGKIMAADVRAALRPADGPARRVQTPLAPRDTRHATRVDNFY
                     LYSLEANMAYLAAISTPIAVQCEKLIGGRYSLFDYVVRAAVKACASEPEWLAGDAAAE
                     LLMVLDKGEKYVFIENASGKTIYNIAMERLAAPAAGPLPAGSVAPNILLCDSGVNVAA
                     QRAVLPEMPHSIISIGGTTPKTGIEAGRPVSKLILPVTLYVNAGILPECKASNIAAEF
                     KTLLENPVLLLL"
     gene            complement(5597..6745)
                     /gene="nagA"
                     /locus_tag="PK077_05055"
     CDS             complement(5597..6745)
                     /gene="nagA"
                     /locus_tag="PK077_05055"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRN23701.1"
                     /translation="MKTLIKNARIISPGIDMPGAAVEMEGKTITAVYPAGSELPAADK
                     VVDAEGRMLLPGFIDIHSHGAGGCDTCDAKLESLRTIAECKMKEGVTSWLPTTLTLSP
                     KVLEDVCASVAEYMKNQEFAKTPGVHLEGPFINPKCCGAQNPAFVRQPDYDEVANLNS
                     IAKVLLVSLAPEMPGAIEFIEKATANGIRCSAGHSAATHEDFNRAKSAGLAHLTHYCN
                     QMSPLHHREIGLVGSGLLDREIKIEIICDTIHLCADMLKTVFKNKDSDQMLMITDSLA
                     CSWMPDGPGDLGGLPIIVKDGVARLQESGALAGSTLKYAHGLKNVHRLTGKPLSDLVK
                     ATSWNQAQSLGLEGLGKIAPGFTADMVLLDDDFNTCKVFIDGVEKYSA"
     gene            complement(6890..7483)
                     /locus_tag="PK077_05060"
     CDS             complement(6890..7483)
                     /locus_tag="PK077_05060"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRN23702.1"
                     /translation="MKKYLTLLLCSLCALACFSASAESTAGLKDVNVVISTTKGDIEL
                     ALYPSKAPVTVANFLNLASRGYYKGITFHRVIPNFMIQGGDPTGTGMGGPGYSFEDEF
                     SPDLRHDGPGVLSMANAGPGTNGSQFFITHVATPHLDGRHTVFGKVLKGQDVVNSIVR
                     GDKIKDIRILDKNAAAAVLKAEAARVARWNKALDAAR"
     gene            complement(7640..>7970)
                     /locus_tag="PK077_05065"
     CDS             complement(7640..>7970)
                     /locus_tag="PK077_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23703.1"
                     /translation="MAASCAALAAVSMASPVFNALLMAAGISAALFLVPLNAFFQDRA
                     DNDKRGDMIAAGNLVDCALGLLAVGFQYAMMLVIPPSWQFVVMGILSLFMAWAVFRFS
                     RAASGSR"
BASE COUNT         1515 a         2114 c         2583 g         1758 t
ORIGIN      
        1 tttttccttt tgccatgttc aagcagggga aggggttaaa acgtgatatt cctgcgtatt
       61 tcctccagct gttcgcgtac ttgccgcagc tgtgccctgc cattcctcct ccatgagggg
      121 acagggcaga aatggaggag cttccggcgg attccggtgt ttttgccggc cctccggagc
      181 cttcttccga agtctgaaaa gccggaggat tgcaacaaca ggcggaaagg aagatgcagg
      241 gaggccctcc ggcactcggc ggccgtgcaa tgggcggtca ggaagtcccg gatgatgctg
      301 taaagtattt cctgctccgg cggggagaaa ggcatgccaa gcagcctcag ttcaaatggt
      361 tcatgctcaa taaagcggac gagatggccg gcttttcttt tccagagatg gcgttgttca
      421 taaaactctt caatcaggga aagccaccgg gggatcatgg gcaggatgtc ggcggttctt
      481 ttccgtatct ggtggtggtt gccctgttca tgctggcggt aatggtaaac ggcctccgga
      541 cagaaggaaa ttttttctgt cagcgcgagc acgaaatgcg agaagaggcc gtcttcacag
      601 gggtgaatgc cttccgggaa ttccagattg tttttcaagg ggaactcccg gcgcagaaat
      661 atgccgcagg tgggcagggc ggggatttta tccggcggaa acctgtcgca gaacagcttt
      721 cccgctacgg tcaaatcgct tgctccgcgg tccgaggcgt ccagaagatg tttgagaaag
      781 gccgcgtcga tgaaatcgtc gccgtcaacg aaacacaaat agcgcccgga agcgcgccgc
      841 atgcccagat ttcgggcgga ggaaacgcct ccatttttct gttcaaaata acggattctt
      901 gagtccgcct ctgaaaaacg ccgggcgatt tcccccgacg agtcggagga accgtcgtta
      961 atgatgaggc attcccactc cgcaagggtt tgccggcgta tgctgctgat gcactcttca
     1021 agatatcgtt cctggttgta aacgggaaca atgacggaaa cctggggctg tggcgggcag
     1081 gattgggtca ttggcgggac tgttgaggtt cggaggggga gcatgcggca agagcccgct
     1141 tcaggttgga gcgggattcc ctgtcaatat ccctgcccaa ttggagaagc gcctgctgca
     1201 tttccgcgta ttcctcctcc gtttgcctga tggcgcggag catggcgccg ccaagcgttt
     1261 cttccccgta aagaagtgcg ttccggtcat tgaagccgta gaaggaagcg aatttctcat
     1321 ggatgaccgg aatcttcgcg aatccgtaaa tgagcagggc ggagccggtg atgccggtgg
     1381 taatgtagcg gtcgtgcgcc ggattgccgg gatcaaggag aggcaggaaa tagtccgccc
     1441 gcctcatggc ctcgtacatg gcggggaagt cgagttttcc ggccacatgc aggaaggggc
     1501 gcaggtggcc gggaatttct cccagttctc cttcccctac gatgatgacg gaaaagtccc
     1561 tgtggccttc atggtagatg gtttgcagcg cttcaatcag gaggttgtgg tttttccgct
     1621 gtgcggaaag ggaacctacg gtaatgaata cgggttcacg gttcctgggc gcaggcggga
     1681 tttccccgaa aaagtgcgga ttgaccatcg tccctttggg aaaggagccc agcgtcagca
     1741 gacgtccctg ccgttcaagc ccttcttccc cgaaccgtgg gatgtcattt aattcatgtt
     1801 cgacggccag aagattggtg aatctgtcaa gccccgtcat gtgaaggaaa cctgcccatc
     1861 ccggagtgtc ataaaaggcg gatgttgata ctaaaatggc ttcgtaccgg ttgaggacgg
     1921 gagctttggc caggcgcttc catgaagtgc acatggctgt gaaaatatgg atatcgtttt
     1981 ccttgaaaca acacaggggc gattgtctgg ccaggctgtg atgcatcagg acatcaacgc
     2041 ggtatcccat gtcgagcagg tggcggcaga aaccggggat gacttccccg tggcaatagt
     2101 tgggttccag aataaggatg gtacggccgg ccactggatg cctgcgccat gcgcgcatgc
     2161 ttcggcgggc gtcccggtca tagtcggaca ggaaatcgcg caggaaagtg cgccattttt
     2221 tacgcaggtt ccgaaacgga atacacatgg tcagcaatcg gagcatgact gtctgcgtat
     2281 gtgtcattgg tttcatggtg aaaaattgca ggaaatataa tgaatatcgc ctgacgcgga
     2341 tgatgaatgg cccttgttac cgttccgggc ggtttttccc ccgccgggta aaaaagaata
     2401 cgttgtgtga cgagggctga atacttaata aattcatact gaaaagcaat atcaaaaaaa
     2461 ctccttggcg catgatgccg gagtattcct gacggtttga attttccgcc gtattgcagc
     2521 cgtaccacag ccctccgttt ccgttattcc cgggaacttc cttcgggagg cagcctttgc
     2581 atttctttct ggaaacagtt tccctcttcc ggttgttccg tttttcaaca gatggcgttg
     2641 ctgttttttc ttgatgtgaa ttttcaatcc ggataaaaaa ggatgcttcc gccatgagcg
     2701 tatccgtttc catcattgtt ccctgttata atgttgccgc ctatttggac caatgtatgg
     2761 aaagcctggc cggtcagagc atggaagata tcgaaatcat ttgcgtcaat gacggttctt
     2821 ccgaccatac ggcggagata ttgcgcgaat ggcgggacag ggacggccgc gtccgggtga
     2881 ttgaccggaa aaacagcggt gtttccgcgg cgaggaacag cggcatggaa gaggctgcgg
     2941 gaaagtatat cggttttgtg gacccggacg acgtggtgga gcggaatatg ttccggcgcc
     3001 tgtttgatgc ggccgtggaa aaagatgcgg acgtagcggt gtgcggatac catgagttct
     3061 gcgaccgcgg aggcatggat atgcctgaaa gcggctggtc gccgtcagcc gggtttttcc
     3121 cggaagaaaa agcggagcgg ttccggcggg ggacgccctg gagccggtgc gcgggaacgg
     3181 tgtggaataa gctgatccgc aggaagctgc tggaagaaaa cggtgtgcgt ttcgttcccg
     3241 gattgcgcca gggggaggat ttgtatttct gtttgatgct gttgactgtt gctccgcggc
     3301 tcctgatcct tcccgaccgt ttgtaccatt accggaggga acgccccggt tccgcctcct
     3361 gcgggcggga tccccgcctg ggggatttcc gcatggattt gatgggcatg gaatctctgg
     3421 cgcgtttctg gggggagcgg gggctccttg attcacccgc cgccttcggt ctggtggact
     3481 actgcatgga gttgatgcgc aggcatttca tcatgaaaga gggagctttt ttcaaagcgt
     3541 ctccggaaga ccgggaagtt ctgctttccg gctggaaaaa gtggctggaa ctggtgggcg
     3601 cggagaacgt ccttcccagt ctggaccggt gggaccgggc tttttgccgc cttctgctgg
     3661 attacccctg ccggagcaat tttttctctg ccctgcattc ccgcctgatg tcgtcccgca
     3721 aggggcgccg gggggcttac tataccctga aaaggcacct gatgaagtga aaaggggaag
     3781 attccccccc tttccctgct gttccggcgc cgcatggggt tgcggaaagg cggaatcctg
     3841 ccggcatcct acaggcccgt gtagcacatc tgctctttga gctgggccac ctggaggtct
     3901 tcccccagat attccccgat ggagtagccg aagagcatgg cggcgccggg gccgttggcg
     3961 gtgagcatgt tgccgtccag caccacattt tcatctttca caatatgggc gccggcttcg
     4021 ttgagttccc ggtaaacgtc ctgggccggg taggccgtga cgttcctgtc cctgaccagg
     4081 cccgctttgg ccagcacgat gggggcggcg cagatggcgg ccaccagctt gcccgcttca
     4141 tgcatttttt tgaccagatg gatgacttcc ggcgtgtcgc ggagtaccca ggagcccgcg
     4201 ccgccgggca ggatcagggc gtccagcttg tcagcatgga gtttatccag catggtgtcc
     4261 gtgctgacgg ttacttcatg ggtgctgacc accttgtcgg actggacgcc ggcaagcact
     4321 acgtccatgt tcaggcgccg caggatgtcc agcggagcca tgagttcgat ttcttcaaat
     4381 ccgggggcgg caagtatggc gacttttttc atggttggta agacaggggg aggaagagga
     4441 acgttcaagg aaaagagcag catgttccgg tatccctaca ggaggagaag aaccgggttt
     4501 tccagcaggg ttttgaattc ggcggcaatg ttggaagcct tgcattccgg cagaatgccg
     4561 gcgttcacgt acagggtgac gggcaggatg agtttggaca cggggcgccc cgcttcaatg
     4621 cccgttttcg gcgtggtgcc tcctatgctg atgatgctgt ggggcatttc cggcagcacg
     4681 gcgcgctggg ctgccacgtt cacgccgctg tcgcaaagaa ggatgttggg cgcgacgctg
     4741 cccgcgggga gcgggcctgc cgcgggggcg gccaggcgtt ccatggcgat gttgtaaatc
     4801 gtcttgccgg aggcgttttc aatgaagacg tatttttccc ccttgtccag caccatgagc
     4861 aattccgcgg cggcgtctcc ggccagccat tccggctcgc tggcgcatgc tttcaccgcc
     4921 gcgcgcacca cgtagtcaaa gaggctgtag cggccgccga tgagtttttc gcactggacg
     4981 gcgatgggcg tgctgatggc ggccaggtaa gccatgttcg cttccaggga atacaggtaa
     5041 aaattgtcta cgcgcgtggc gtggcgcgtg tcccggggcg ccagcggggt ctgcacccgg
     5101 cgggcggggc cgtccgcggg gcgcagggcg gcccgtacat ccgcggccat gattttgccc
     5161 cgggggccgg tgccgcgcag cgtggcgggg ttcaggccgg cttccgccgc cagcctggcg
     5221 gccagcgggc tgcatattgc ggacgggcgc ggggggcagc cgcagacggc tgcgccgaat
     5281 tccggctgaa gcagcgcaat gggcgctccc gccgcggcgg aacctccctc cggcaccagg
     5341 atttccgtca gggtgccgtc ctcgcaggcc tggagctcca catgggcctt gtccgtctca
     5401 atatcggcca gatggtcgcc gacttttacg aagtcccctg tttttttgaa ccatctcagg
     5461 acggtgcctt cgtgcatgga gtcggacagg cggggcattt caatggtaat agccatggag
     5521 gtgaatgttg aaatatggta aaagggagga gcgccggaag gggcgggacg tctccttccg
     5581 gcgcgcggaa acgcggttac gcgctgtatt tttccacgcc gtcgatgaat accttgcagg
     5641 tattgaagtc atcgtccagc agaaccatgt ccgccgtgaa gccgggggcg attttgccca
     5701 ggccttccag gcccagggac tgcgcctggt tccaggaggt ggctttcacc aggtcgctga
     5761 ggggtttccc ggtcaggcgg tgcacgtttt tcaggccgtg ggcgtatttg agggtgctgc
     5821 cggccagggc gccggattcc tgcagacggg ccacgccgtc cttcacgatg atgggtaggc
     5881 cgcccaggtc tccggggccg tccggcatcc aggagcaggc cagggagtcc gtaatcatca
     5941 gcatctggtc agaatccttg tttttgaaca cggttttgag catgtccgcg cagaggtgga
     6001 tggtgtcgca gatgatttca atcttgattt ccctgtccag caggccggac cccaccaggc
     6061 cgatttcacg gtggtgcagg ggggacatct ggttgcagta gtgggtcagg tgcgccagcc
     6121 ccgcggactt cgccctgttg aagtcctcat gggtggcggc gctgtgcccg gcggagcagc
     6181 ggatgccgtt ggccgtggct ttctcaatga attcaatggc tccgggcatt tccggcgcca
     6241 gggagaccag cagcaccttg gcgatggagt tcaggttggc tacttcatcg taatccggct
     6301 ggcggacaaa ggcgggattc tgcgcgccgc agcatttggg gttgatgaac ggcccttcca
     6361 ggtggacgcc gggagtcttg gcgaattcct ggttcttcat gtattccgcc acggaggcgc
     6421 atacgtcttc cagcaccttg ggggacaggg tgagggtggt cggcagccag gaagtgacgc
     6481 cttccttcat tttgcattcc gcaatggtgc ggaggctttc cagcttggcg tcgcaggtgt
     6541 cgcagccgcc ggcgccgtgg ctgtggatgt caatgaagcc gggaaggagc atgcggcctt
     6601 ccgcatccac gaccttgtct gcggcgggaa gttcgctccc ggcgggatag acggcggtaa
     6661 tggttttgcc ttccatttcc acggcagccc cgggcatatc aatgccgggg gagatgatgc
     6721 gtgcgttttt aatgagtgtc ttcataaaaa tgaatggttg ttggatggat gtttatcgtc
     6781 gtttttctcc gttcgtccgc gtcttcccgc ctggcctccg cttcctgcgg aaaaccggga
     6841 atgggcggcg gcgcaccgga gatcgttcat gccgctggca gcttcctggt tatctggcgg
     6901 cgtccagggc cttgttccac cgggctacgc gggcggcttc cgctttcagc acggcagccg
     6961 cggcgttctt gtccagaatg cggatgtcct tgattttgtc cccccggaca atggagttga
     7021 cgacgtcctg ccctttcagc accttcccga agaccgtatg gcggccgtcc agatggggcg
     7081 tggccacatg ggtgatgaag aattgggaac cgttggtgcc ggggcccgcg ttcgccatgg
     7141 aaagcacgcc ggggccgtca tgcctgaggt ccggggaaaa ctcgtcttca aaactgtagc
     7201 cggggcctcc catgcccgtg ccggtgggat cgccgccctg gatcatgaaa ttcggaatca
     7261 cgcggtggaa ggtgatgccc ttgtagtacc ctctgctggc gaggttcagg aagttggcta
     7321 cggtgacggg cgccttggag gggtaaaggg ccagttcaat atcccctttg gtagtggaga
     7381 tgacgacatt gacgtccttg aggccggctg tggattccgc ggaggcgctg aagcacgcca
     7441 gggcgcacag ggaacacaac agaagggtga gatatttttt catggctttt tgttttcccc
     7501 cctatgttgc ccgaataaaa gcagggttgc aagtaaatcg cgcgccctga cgggagaatg
     7561 gcgcggggcg cgccaatgcg ggaaggtttt tctctgaaga gggattttta aggatgggcg
     7621 cccggttgcc ggcccaggac taccttgagc cggaagccgc gcgggaaaaa cggaagaccg
     7681 cccacgccat gaagaggctg aggattccca tgacgacgaa ttgccaggag ggggggatga
     7741 ccagcatcat ggcgtattgg aacccgacgg ccagcaaccc gagcgcgcag tccaccaggt
     7801 tgccggcggc aatcatatcc ccgcgtttgt cattgtccgc ccggtcctgg aaaaaggcat
     7861 tcagaggcac caggaacagg gcggcggaaa tgcccgccgc catcagcagg gcattaaaga
     7921 ccggggaagc catggagact gcggccaggg ccgcgcagct cgccgccatc
//
LOCUS       DAOMSU010000084         7821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_709_length_7821_cov_2.660057, whole genome shotgun sequence.
ACCESSION   DAOMSU010000084 DAOMSU010000000
VERSION     DAOMSU010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_709_length_7821_cov_2.660057"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1321)
                     /locus_tag="PK077_05070"
     CDS             complement(<1..1321)
                     /locus_tag="PK077_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23704.1"
                     /translation="MIETITEEQLTPQQAEFWVRARQAVDMNNYPYAVSLLKALVKQL
                     PGFLEGRKALRACEIKLNPEAKRGGLFSGMKISTSKLTSSKKDAATQLSALEDELEND
                     PYSIPVNEALYTAAMEVNFPDLAAFALETVRQGHPGNKKMLHMLASHYVSRDMPAQAA
                     EVYHDLVKLDPTDSVAVKSEKDCMARATMQQQKWEEAKSFRDVMKNSSETNTLDKSDK
                     KGLTRAELEERLGLLSARYAQNQQDLAVVRDIAGVYEQMEDWANAYSFYNYAFSLSNN
                     DISLENKASEMNERCRKAQVEEIRRRAAAEPDNKELQEQLARFSKEAAEQQVALCRQR
                     VENNPTDPQTRFELGQALFDCGNYTEAIPELQRARNNPHIRIRAMLLLGKCYDAKNMH
                     DMALRQLEEANKELIEMNDTKKEILYMIGLLYEKQGKKGESLAAFQQI"
     gene            1458..2150
                     /locus_tag="PK077_05075"
     CDS             1458..2150
                     /locus_tag="PK077_05075"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRN23705.1"
                     /translation="MRLDALLSRYGYCSRREAPGWIKRHSITFKGKPCSSPTDKVQAE
                     GILLDGEPVEFPDGLYAALYKPEGYTCSHDDGEGDLIYDLLPFQWRHRNPAVSSVGRL
                     DKETSGLLLLTDDGKFIHRMTSPRHHVAKVYEAVTEEDIPTEAVELFASGTLTLNGET
                     RPCAPALLEIREPRRALLTLTEGKYHQVRRMLAAVGAPVLSLCRVSIGSLHLDSLNLK
                     PGEWAPIRPDAL"
     gene            2165..2941
                     /locus_tag="PK077_05080"
     CDS             2165..2941
                     /locus_tag="PK077_05080"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRN23706.1"
                     /translation="MWIPISEAPIACFPHFEVAAESGDWIVVDKGAPLIVHPSNGKKE
                     PCLLEGVEALLSYEIANGAALSLVNRLDRETSGLTLVAKNKRAARELGRAMQRRLIHK
                     EYLAIVQEWPEWTETACTAPILRQGEVAESRIWVKQAVHPAGKACTTVFRKERTWNTR
                     RGPLALVRCIPETGRMHQIRVHLTHLGHPILGDKIYGPFEHCYLEYIQHGWSRELEEQ
                     LVLPRHALHACRLEFPFDEETFTAEAPLPEDMRLLLETGD"
     gene            complement(2954..3601)
                     /locus_tag="PK077_05085"
     CDS             complement(2954..3601)
                     /locus_tag="PK077_05085"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRN23707.1"
                     /translation="MNMNAQELIETLEWRYATKVFNPDRRIPAEDWEALLESLHLSPS
                     SLGLQMWKFLDVRDPAVRSRLREVSWGQPQVTDASHLVVFCARRDFKPEDVQRYLERI
                     VEVRGVAMDSLDQYRARIFELVGSKSPEVLKAWLERQVYIALGFMMSCAADLRVDTCA
                     LEGMDPAAYDRILYLENSPYYTLCALALGYRDEEADKYARLAKVRFDRDEVIPVI"
     gene            complement(3948..>4723)
                     /locus_tag="PK077_05090"
     CDS             complement(3948..>4723)
                     /locus_tag="PK077_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23708.1"
                     /translation="RRYSSVYSDSFGLNGMNHIVQPHFTLAYVKTNRLSELYPQIDGD
                     TPTTNPPSLSIGRYTEIDSLSTGLVFRYGLRNMLMTSRDANSHRWFSWDVFMDAYLHD
                     PVNQRDFSNLFSFMRWNPVPWMEYRSEMQAPVLGKDKISGCREYNNSLRFMPWRSTEL
                     VVGHRYLNQHSLLEDSSQLDLRILQRFSEAWAFSGKWRFSLLDGKLDIQEYNVYHNMG
                     SWYLGVGAFVRKNGNKNEFGLGISFTIQQTGDYMPVKFL"
     assembly_gap    4724..4764
                     /estimated_length=41
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4765..6376)
                     /locus_tag="PK077_05095"
     CDS             complement(<4765..6376)
                     /locus_tag="PK077_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23709.1"
                     /translation="MIGTIAKLWGAAALVAGASLFAETAAEQPPVPSSETEQENAGGK
                     MAGNLLSGTLMPQIPSNISITNDGAIRVEESRKVIFEGPHVHMVTDTGVEVFADYAQA
                     DMDQGKVFLKGNLAIYQGDGRTRTNSLVRADSAEFDWENEVLLTDAIRAKMEGLILRS
                     GKFESRKDAAGNTYLEALNASVTAEDISEPLTWISADRIRVYPEDRFSFKNLTLYYGG
                     VPFFYFPYLSHSLNPEVGYLPIPGMRSIWGPYLLNEYGFLMGKKWSDNGMPSADYLGT
                     LHADYRTRRGFAYGLDIRDVLLEKDCPDMTGLSFYKTHDKGVKINAGDDEYREHLDPD
                     RWRFALQQMWSHRVNLRTDWRLKANINMLSDEYMLRDFYPEIYQRNSSPDNTVLLSRT
                     DDTNDFSLLQRFVPNNFYIADQRTEFSYERIKSPVFRSPVMYESRTSFAFLKQYVPPF
                     MRTEIRNMLDGMDPGTSSYDYWARMLMTDSYSRFHSFHEVSVSKKIMGFLNLTPKVGG
                     GYTGYYGVEDYKPLNQGIFYAGTDADFKF"
     gene            complement(6456..>7821)
                     /locus_tag="PK077_05100"
     CDS             complement(6456..>7821)
                     /locus_tag="PK077_05100"
                     /inference="COORDINATES: protein motif:HMM:NF019908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NPCBM/NEW2 domain-containing protein"
                     /protein_id="HRN23710.1"
                     /translation="VVCQLPDGVGPYYGGGFSHQGTGWTCDQEGLDPASFLDTEMMQG
                     GRFKVTRGKNATIYIGGTAHELGHSFGLPHTGDGWNYPDAGASLMGHGNSTYGDELRH
                     EGKGAYLAPTDALKLASVPLFNGVETELPADASFGRMLGKYVPGSFERLEAIPVKDGL
                     RLKGRVHLTRPAYGIVAHLDPPGGSDYDSNAVGASLDEKGEFDLTICRPGYKGGFIEM
                     RVAVLNCDSTRSMITLPVWMDARGAKAPSLAQIVYFGDVQNLWIRGRTEEARKALAEV
                     ERRHGSRSEVKEWLPVWKRALGRQEPALEVVPAQIPAATASISLNDCKPSVAQVGWAV
                     PLWDVLFPSDLGPVPFFRTIGRPERFILAHAPAVFAYDLDGRWKEFRADVGLPFGSRG
                     SVKFSVYLDGRKLMESPVLKDGDSIPVKTAVEGGRKLEIRVDDAGDGNAADWGIIANG
                     MLTR"
BASE COUNT         1750 a         2302 c         2085 g         1643 t
ORIGIN      
        1 aaatctgctg gaatgcagcc agggattccc ccttcttgcc ctgcttttca taaagcaagc
       61 cgatcatgta aaggatttcc ttcttggtgt cattcatttc tatcaattcc ttattggctt
      121 cctccagctg gcgcagggcc atatcatgca tgttcttggc gtcatagcac ttgccgagca
      181 acagcatggc gcggatgcgg atatgggggt tgttgcgggc acgctggagt tccggaatgg
      241 cttccgtgta attgccgcag tcaaagaggg cctggcccag ctcaaaacgg gtttgcgggt
      301 ccgtggggtt gttttccaca cgctggcggc acaaagccac ctgctgctcc gcagcttcct
      361 tgctaaaccg ggcaagctgt tcctgaagtt ccttattatc cggctccgcg gcagcgcggc
      421 ggcggatttc ctccacctgg gccttgcggc agcgctcatt catttccgag gccttgtttt
      481 ccagggaaat atcattgttg ctgaggctga acgcgtaatt atagaaggaa taggcattag
      541 cccaatcttc catttgttca taaacgccgg caatgtcgcg cacgacggcc agatcctgct
      601 ggttctgggc gtaacgggcg gaaagaagcc ccaggcgctc ttccagttcc gcacgggtga
      661 gccctttctt gtcgctcttg tccagggtgt tcgtttccga tgagttcttc atcacgtccc
      721 ggaagctttt agcctcctcc cacttttgct gctgcatcgt ggcgcgcgcc atgcagtcct
      781 tttcgctctt tacggccaca ctgtccgtgg gatccagctt cacaaggtca tggtacactt
      841 ccgcagcctg ggccggcata tcccgggaaa cgtaatggga ggccagcatg tggagcattt
      901 tcttgttgcc gggatgcccc tggcgaacgg tctccagagc aaaagcggcc agatccggga
      961 aatttacttc catggcagcc gtgtacaggg cctcgtttac cggaatgctg taaggatcat
     1021 tttccagttc gtcttccagc gcggaaagct gggtagccgc gtcctttttg gaagaagtaa
     1081 gcttgctggt gctgattttc atgccgctga acagcccccc tctcttggcc tccggattca
     1141 atttgatttc acaggcgcgc aacgccttgc ggccttccag aaaaccggga agctgcttta
     1201 ccagggcctt gagcaggctg acggcatagg gataattgtt catgtccacc gcctggcggg
     1261 cgcgcaccca aaattcggcc tgctggggag tcaactgttc ttctgtaatc gtttcaatca
     1321 tggaactata taagcgtaaa tggtgaacaa cggcagcgcc ccaccagggc ggctccgcat
     1381 gtaatagttt aatgctatca gccggggagg catacttcaa ccttgttttt tgttcccgtt
     1441 aggaaaaaat atgccgcatg agactggatg cccttttatc ccgctacgga tattgttcca
     1501 ggagggaagc gccgggttgg atcaaacgcc actccattac attcaaggga aaaccatgct
     1561 cctcccccac ggacaaggtg caggcggaag gcatcctgct ggacggagaa cctgtggaat
     1621 tcccggacgg cctgtacgcc gccctttaca aacctgaagg gtacacctgc agccatgacg
     1681 acggggaggg ggatttgatt tacgacctgc tgcccttcca gtggcggcac aggaaccctg
     1741 cggtttcttc cgtaggacgg ctggacaagg aaacttccgg cctgcttctc ctcacggacg
     1801 acggaaaatt catccaccgc atgacctccc cccgccatca cgtcgccaaa gtttacgaag
     1861 ccgtcacgga ggaggacatt ccgacagaag ccgtggagtt gttcgcctcc ggaaccctca
     1921 cgctgaacgg ggaaacccgc ccctgcgccc ccgcccttct ggaaatccgg gaaccgcgac
     1981 gcgccctgct gactcttacg gaaggaaaat accaccaggt tcgccgcatg ctggctgccg
     2041 taggcgctcc cgtcttatct ctgtgccgtg tttccatcgg ctccctgcat ctggacagcc
     2101 tgaacctgaa accgggggaa tgggcgccta tccgtccgga tgcgctgtaa acctcccatc
     2161 cgccatgtgg attcccattt ccgaagcacc aattgcctgt tttccgcact ttgaggtggc
     2221 ggcggaatcc ggggactgga ttgtggtgga caagggagcg cccctcatcg tccacccgtc
     2281 caacgggaaa aaggaaccct gcctcctgga aggcgtggaa gccctgctaa gctatgaaat
     2341 agccaacgga gccgcccttt ccctggtcaa cagactggac agggaaacca gcggtctgac
     2401 gctggtcgcc aaaaacaaaa gggcggccag ggagctggga agagccatgc agcggcgcct
     2461 gatacacaag gaatatctgg caatcgtgca ggaatggcct gaatggacgg aaacagcatg
     2521 caccgccccc attttaagac agggggaagt cgcggaaagc cgcatatggg tcaaacaggc
     2581 cgtgcaccca gccggaaaag catgcactac cgttttccga aaagaacgga catggaatac
     2641 acgccggggc cccctggcgc tggtccggtg cattccggaa acaggacgca tgcaccagat
     2701 acgcgtacat ctgacgcact tgggacatcc catcctgggt gacaaaattt acggtccgtt
     2761 cgaacattgc tatctggaat acatccagca cggatggagt cgggaactgg aggagcagct
     2821 ggttctgccg cgccatgcgc tgcatgcctg ccggctggaa tttccgtttg atgaagaaac
     2881 cttcaccgcg gaagcccccc tgccggaaga catgcggcta ctgcttgaaa ccggggatta
     2941 gtaaaaaact ccgtcagatg accggaatga cttcatcccg gtcaaaacgt actttcgcca
     3001 gacgggcgta cttatccgcc tcttcatctc ggtaacccag agccagggcg caaagcgtgt
     3061 aataagggga attttccaga taaagaatgc ggtcataggc ggccggatcc atcccttcca
     3121 gcgcgcaggt gtccacgcgg agatccgcgg cgcagctcat catgaacccg agggcgatgt
     3181 acacctgccg ttccagccag gctttcagca cttccggaga cttggaaccc accagttcaa
     3241 aaatgcgggc gcggtactgg tccagggagt ccatggcaac accgcgcact tccacaatac
     3301 gttccagata gcgctgcacg tcttccggct tgaaatccct gcgggcacag aaaaccacca
     3361 gatgggaagc gtccgtaacc tggggctgcc cccatgaaac ttccctaagc cgggaacgca
     3421 cggcgggatc ccggacatca aggaacttcc acatctgaag ccccagggag gaagggctca
     3481 aatgcaggga ttccagaagg gcttcccagt cctccgccgg aatgcggcgg tccggattga
     3541 ataccttggt ggcgtaacgc cattccagcg tctcgatcaa ttcctgagcg ttcatattca
     3601 tgtatgatta ttcgtgcagg aagccggacg gttttccgcc gcagcttgct ccatgccttg
     3661 gcattttccc cgtttccctg ccggaatgca agccaaggtt ccggcacaag cgcggcagcg
     3721 gctccttacc tcttcttcct ttcttcctgt ccggcacgcc ggaaacaggt cgctctccat
     3781 ccttccaacc gcatggcttt ccgcctcttg ccgaccagct acatatcttt ccctttccgg
     3841 aacaacaccc cgcaccgggc cgaccccatt ctcgaaacgc gaatcctctg aaaaaggaaa
     3901 aagcttccgg gaaaaacctc gcaaaaaaac cgtgtccttt ccggacatca aaggaatttg
     3961 accggcatat aatccccggt ctgctggatg gtaaagctga tgcccagccc gaattcgttt
     4021 ttgttcccgt tcttgcgaac gaaagcgccc acgcccaaat accaggatcc catattgtgg
     4081 tacacattgt attcctgaat gtccagcttg ccgtccaaca gggaaaaacg ccacttgccg
     4141 ctgaaagccc aggcttccga aaagcgctgc aaaatacgca gatccagctg gctgctgtcc
     4201 tccagaaggg aatgctggtt caaataacgg tgccccacga cgagctccgt ggaacgccat
     4261 ggcatgaaac gcagggagtt gttgtattca cggcagccgg aaatcttgtc cttacccaaa
     4321 acgggagcct gcatttccga ccggtattcc atccagggaa cgggattcca gcgcatgaat
     4381 gaaaaaaggt tggaaaaatc ccgctggttg acgggatcat gcagataggc atccatgaac
     4441 acatcccagg aaaaccagcg gtgggaatta gcatcccggc tggtcatcag catgttgcgc
     4501 aatccatacc ggaagacaag ccccgtggac agggaatcaa tctccgtgta tctgcctatg
     4561 ctcaaggatg ggggattggt cgtgggggta tcgccgtcaa tctggggata aagctcgctc
     4621 aggcggttgg ttttgacata cgccagcgta aaatggggct gaacaatatg gttcatgccg
     4681 ttcagcccaa aggaatcact atatacggaa gaataacggc gggnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnagaatt tgaaatcggc atccgttccg gcgtaaaaaa
     4801 ttccctggtt gagcggtttg tagtcctcca cgccgtaata tcccgtatag cctccgccca
     4861 ctttgggggt caggttcagg aatcccatga ttttcttgga aacggaaacc tcatggaagg
     4921 agtggaacct gctgtagctg tccgtcatca gcattcgggc ccagtaatca taggaagaag
     4981 ttccggggtc catcccgtcc agcatgttcc ggatttccgt ccgcatgaac ggaggaacgt
     5041 actgcttgag gaaagcgaag ctggtgcggc tttcatacat gacgggggaa cggaatacgg
     5101 gggacttgat acgctcataa ctgaactccg tccgctggtc cgctatgtaa aaattattgg
     5161 ggacaaaacg ctgcaacagt gaaaaatcat tcgtatcgtc cgtacgggaa agaagcaccg
     5221 tgttgtccgg agaagaattg cgctgataaa tttccggata aaaatcgcgg agcatgtact
     5281 cgtcgctcaa catgttgatg ttggccttca accgccagtc cgtgcggaga ttcaccctgt
     5341 ggctccacat ctgctggagc gcgaacctcc accggtccgg atccagatgc tcacggtact
     5401 catcatcacc ggcgttgatt ttcacaccct tgtcatgggt tttgtaaaag gacagccccg
     5461 tcatgtccgg acaatccttt tccagcagca cgtcgcggat gtccaatccg taggcgaaac
     5521 cgcgccgggt gcggtaatct gcatgcaggg ttcccaggta atccgcggaa ggcataccgt
     5581 tgtccgacca ttttttcccc atcaggaacc cgtattcatt caagaggtaa ggcccccaga
     5641 tggagcgcat gccggggatg ggaaggtagc caacttccgg attaagggaa tggctgaggt
     5701 aggggaaata aaagaaggga acgccaccgt aataaagggt caggttcttg aaagaaaacc
     5761 tgtcttccgg atacacgcgt atcctgtccg cggaaatcca ggtgagaggc tcgctgatgt
     5821 cttccgccgt gacggaggca ttaagggctt ccagataagt attgccggcg gcatccttcc
     5881 gggattcaaa ctttccgctg cgcagaatca atccctccat cttcgcacgg atggcatccg
     5941 taagaagcac ttcgttctcc cagtcaaact ccgcgctgtc ggcgcgtacc aggctgttgg
     6001 tccttgtcct gccgtcaccc tggtaaatgg ccaggttccc tttcagaaac accttgccct
     6061 ggtccatatc cgcctgggca taatccgcaa atacctccac cccggtatcc gtcaccatgt
     6121 ggacatgcgg tccttcaaaa ataaccttcc tggattcctc caccctgatg gcgccgtcat
     6181 tggtaatgga aatattggag ggaatctgcg gcatgagagt gccggaaagc agattgcccg
     6241 ccattttccc tccggcattt tcctgctcgg tttcagagga agggacgggc ggctgctccg
     6301 cagccgtttc tgcaaacagg gaagccccgg caaccagcgc cgccgcaccc catagctttg
     6361 caatcgtccc aatcatggct agcccacaga acactatgag aaagggcaaa agacaagcct
     6421 aatgtcacat ttctccgcca gtgggggcag aaaatttacc gggtcagcat gccgttggct
     6481 ataatgcccc aatcggcggc attgccgtct ccggcgtcat ccacgcggat ttccagcttc
     6541 ctgccgccct ctaccgccgt cttcacggga atggagtccc cgtccttgag cacaggggat
     6601 tccataagct tcctgccgtc caaatacacg ctgaatttga cgctgccgcg ggagccgaag
     6661 ggcagcccca catcagcgcg gaactctttc cagcgcccat ccaggtcata ggcaaaaaca
     6721 gccggggcat gagccagaat gaaacgttcc ggccttccga ttgtccggaa gaaaggcacg
     6781 ggccccaaat ccgacgggaa caggacatcc cacaacggaa cggcccaccc cacctgggct
     6841 acggaaggct tgcagtcatt caggctaata ctggcggttg ccgcaggaat ctgcgccgga
     6901 acaacttcca gcgcgggctc ctggcgcccc agagcgcgct tccagacagg gagccattct
     6961 ttcacttcag aacgggaacc atgcctgcgt tccacttccg ccagcgcctt ccgggcttct
     7021 tccgtgcggc cccgtatcca cagattctga acatccccga aataaacgat ctgggccaga
     7081 gaaggggcct tggctccccg ggcatccatc cacacgggca gagtaatcat gctgcgcgtg
     7141 ctgtcgcaat tcaatacggc cacccgcatt tctataaaac cacccttata tcccggcctg
     7201 cagatggtga gatcaaactc tcccttttca tccagagaag cgcccacggc attggaatca
     7261 taatccgaac ctccgggcgg atccaaatgg gcaacaatgc catacgcggg gcgcgtcaga
     7321 tgaaccctcc ctttcagccg caatccgtct ttaacgggaa tggcctccag ccgttcaaaa
     7381 gaccccggga catacttgcc gagcatacgc ccgaaggagg cgtctgcggg aagttccgtc
     7441 tccaccccgt tgaacagggg aacactggcc agtttcaaag catccgtcgg cgccaaatag
     7501 gcacccttcc cttcatggcg cagctcgtcg ccgtaggtgg aattgccatg gcccatcagg
     7561 gaagctccgg cgtcggggta attccatccg tcgcccgtgt ggggaaggcc gaaggaatgc
     7621 cccaattcat gggccgtacc gcctatgtaa atggtggcgt tctttcccct ggtcaccttg
     7681 aaacggccgc cctgcatcat ttccgtatcc aggaagctgg cgggatccag cccttcctgg
     7741 tcacaggtcc accccgtgcc ctggtggctg aagccgccgc cgtaataagg acccacgccg
     7801 tccggaagct ggcaaacgac c
//
LOCUS       DAOMSU010000085         7758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_723_length_7758_cov_2.477217, whole genome shotgun sequence.
ACCESSION   DAOMSU010000085 DAOMSU010000000
VERSION     DAOMSU010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7758
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_723_length_7758_cov_2.477217"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="PK077_05105"
     CDS             complement(<1..805)
                     /locus_tag="PK077_05105"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRN23711.1"
                     /translation="MLDAGASSGKDKAMQTELFEVDPLSDNRKLYARAAEALGAGALV
                     GIPTETVYGLGADALNPEAVARIFEAKGRPSFDPLIIHVHHGCEVEKYTAVPEGLKDL
                     VHTLASRFWPGPLTLVLPKADIIPDIVTSGLPTVAVRVSAHPAMRGVAKALGRPIAAP
                     SANRFGHISPTSASAVQKELGGSIEMILDAGACSEGLESTIVRPVLDEKGKPSLELLR
                     EGPVTREQFRNLVKVVRRKPSCRPVSEESPADAPGQLTSHYAPRKPMLLL"
     gene            1004..1558
                     /locus_tag="PK077_05110"
     CDS             1004..1558
                     /locus_tag="PK077_05110"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HRN23712.1"
                     /translation="MNKAASTSLPNIILIGLMGCGKTTIGKELHRETNLGFTDTDQLI
                     ERQTGMHIPQIFKIHGESHFRDLETGILQQLQTAAAQSRIISTGGGIIIRPENRLLLK
                     KLGFVVWLHTDVNILYQRISRCTNRPLLQQPNPKAVLEHLMKERHDFYRETAHLTIDT
                     ANLHIHEIAFGILESARVFRSRRQ"
     gene            complement(1722..1964)
                     /gene="rpsP"
                     /locus_tag="PK077_05115"
     CDS             complement(1722..1964)
                     /gene="rpsP"
                     /locus_tag="PK077_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRN23713.1"
                     /translation="MAVAIRLNRQGSKDRPYYKIVVVDSRARRDGRYIEQVGSYDPMK
                     EGVNYTINLEKVDKWLANGAQPSETVNSMIRKARKA"
     gene            complement(2130..2756)
                     /locus_tag="PK077_05120"
     CDS             complement(2130..2756)
                     /locus_tag="PK077_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HRN23714.1"
                     /translation="MKEELIFAGILLLSQASPGPDQAFVTRSTLAYGFGAGVMAALGI
                     GTGIVAHAALACTVGASVFHSFLGLGLFCAASCWMLYLAWKIWPRGRNAFLAGCGDIP
                     PASCIYRDALVTNLMNPKATFFFVALSAPLLEKNHDPSYALLIGFLIVATGTVGWILW
                     ALVFRWQPIRSFYGRYAGILDGILSLVLAGFGFSMLYSFGRMAVENFS"
     gene            complement(2771..3673)
                     /locus_tag="PK077_05125"
     CDS             complement(2771..3673)
                     /locus_tag="PK077_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HRN23715.1"
                     /translation="MMGIPLEPEQGFLQYLEVEKQASPHTVEVYARALRQFRAWAADS
                     FPGWENCTPDQMRDWLFQELKDEAATSSIRLRFAALRSFYRFMMRRHGLETNPMTGVS
                     LPRRKKTLPVFLTLNQMLELLELPYKTAVPANAPAWLPYRDAAILELFYSCGMRLSEL
                     VGLDVGSVDHRFRGVKVMGKGRKERILPVGTPALAALETYVSMACLPKNSPLFVSRIG
                     TRLSARSVQMMLNKYVKLSSIPFTISPHKIRHTFATHILEAGADLRSVQELLGHASLS
                     TTQIYTHVTRARMAEVYRQAHPRA"
     gene            complement(3684..4547)
                     /locus_tag="PK077_05130"
     CDS             complement(3684..4547)
                     /locus_tag="PK077_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HRN23716.1"
                     /translation="MRLPHMFILSCSAALAFGLFPFAVGQSVAFGDRGKMETAPRPGL
                     ECLQVTLLIRQMVLDRYDTTGTGILSEQDKARLVEDARSARREARKAFLRKFDKDGDG
                     RLSPEEYRAFREHVEKRRGVRRPDAGPDKRDGIPFYPPAERKEIPPPPPPAEGGKVPM
                     VEIRTVGRKRFMVAPGLFLLTRNMLLQNYDANGNGRIDPEEHAVMKDAAALYQAKMKE
                     MMELYDLDQDGVLSPVEREQALADSHKDSPLYAMEEPDDIDLFIRANISEVLLKESPV
                     DAGDDKDKGTE"
     gene            complement(4657..4923)
                     /gene="yajC"
                     /locus_tag="PK077_05135"
     CDS             complement(4657..4923)
                     /gene="yajC"
                     /locus_tag="PK077_05135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HRN23717.1"
                     /translation="MFQQILASPMFMFVIIIVLFWVMLIRPQRKAQKEQQARIAALQR
                     GDKVITNAGLHGFVEKVNERTVSLKIAEGVVIELEKNAIVQVEK"
     gene            complement(4986..6119)
                     /gene="tgt"
                     /locus_tag="PK077_05140"
     CDS             complement(4986..6119)
                     /gene="tgt"
                     /locus_tag="PK077_05140"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRN23718.1"
                     /translation="MPFILRQKDSSTAARLGSLELPHGRVPTPIFMPVGTQGSVKTMH
                     PQDLEMLGAEIILGNTYHLSLRPGSALIRQMGGLHKFSSWNRPILTDSGGFQVWSLAK
                     LRKITEEGVRFQNHLDGAYMMLSPERSMEIQADLGSDIAMLFDECPPYPCDRKYAEAS
                     LGYTLRWARRCKAWVQEHRPRSGEGRQHHFGIVQGSVYADLRKKCAEELAAMDFDGYA
                     IGGVSVGEPEDEMLRAIDHSAPWLPEEKPRYAMGLGTPPQLLEMIARGVDMFDCVMPT
                     RLARHGVALTPDGPMHIKNQRWATDSRPIDPEGHPHVTQFSRAYVRHLFKAGEILALR
                     LLSFQNLEFYLRLMAQAREAIAAGTFGSFKDSFIARYKANDIL"
     gene            complement(6125..6721)
                     /gene="trmB"
                     /locus_tag="PK077_05145"
     CDS             complement(6125..6721)
                     /gene="trmB"
                     /locus_tag="PK077_05145"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HRN23719.1"
                     /translation="MINPAFIPDDFFKVLAPSDIFGADGPFEVDLGCGDGGFLLQMAA
                     HYPERRFLGIERLLGRVRGVCSRAAVRGLDNVKVFRVESRYFLEWMMQPGCISRLHYL
                     CPDPWPKERHHKNRLVQEDFLPVLHRSLSDGGEFLFKTDHEGYFLWVLDHVARSGLFS
                     RASWDEDEFFYPRTDFQLQWEAMGKPIYRARFIKLQKV"
     gene            complement(6850..7572)
                     /locus_tag="PK077_05150"
     CDS             complement(6850..7572)
                     /locus_tag="PK077_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRN23720.1"
                     /translation="MSRQAFYPHLSVDCVLIGFDEEGLKVLLVEKTQFEPGHTGAISK
                     LPGDLIYEEEELDAAARRVLFDMTGMSSPHLEQFHTFGAPSRIKNPEDRAWVEAVSGQ
                     KIGRLVTVAYMAMLRISTKLRKLMESHKTRWVPVDDLPELAFDHRDIIDLALERVRSS
                     VKKEPALIYDMLPAKFTALQLRRLNEEIHGKPMDVRNFHKKIASRPYIVPLDEKEEGV
                     PHRAARYYRFDRKIYNRLYCRS"
BASE COUNT         1971 a         2299 c         1909 g         1579 t
ORIGIN      
        1 ccagcagcag cataggtttg cgcggggcgt aatggctggt gagctgcccc ggcgcatccg
       61 ccggagattc ttccgaaaca ggacggcagg aaggcttgcg gcgcaccacc ttgaccaggt
      121 tgcgaaattg ttcccgggtc accggcccct cacgcagcaa ttccagagag ggcttgccct
      181 tctcgtcaag cacagggcgc acaatcgtgc tttccagccc ctcggaacag gctccggcat
      241 ccagaatcat ttcaatactg ccccccaatt ccttttgcac ggcagaggcg gaagtagggc
      301 tgatatggcc gaagcggttg gcgctgggcg cagctatagg acgccccagc gctttcgcca
      361 ccccccgcat ggcaggatga gcgcttacgc gaacggccac cgtaggaagg ccgctggtta
      421 caatatccgg aatgatatcc gcctttggca acaccaaggt cagcggcccc ggccagaatc
      481 tggaggcaag cgtatgcacc agatctttca gcccctccgg aacggcggta tatttttcca
      541 cctcacaccc atgatgcaca tgaataatca gcggatcaaa agaaggcctt cccttggctt
      601 caaaaatcct tgctaccgct tccgggttca aggcatctgc ccccaggccg tatacagtct
      661 ccgtgggaat ccctaccagt gctcccgcac ccagagcttc cgccgcacgg gcatacagtt
      721 tgcggttgtc tgacagggga tctacctcga acaattcggt ttgcatggct ttatccttgc
      781 ctgaagaagc tcctgcgtca agcaagacct ccttccctgg aagggagaaa acagacaaat
      841 gaataaaagc acatcctgcg gcctcatact tcccctctct ttttccaact gcctttactt
      901 cactccaccc ttacggactt ccccgagtga aagccggcgc tggaaaaggc cattccgcta
      961 aaaagttgtt ttctcacgcc gccggattga gtacaatcaa gccatgaata aggccgcatc
     1021 aacatcctta ccaaacatca tcctgatcgg gctgatggga tgcggcaaaa ctaccatcgg
     1081 aaaggaactg caccgggaaa cgaaccttgg ttttacggat acggaccagt taatcgaacg
     1141 ccagacggga atgcacattc cgcagatttt caaaattcat ggggaatcac attttcgaga
     1201 cctagaaacc ggaatcctgc aacagcttca gacggccgcc gcacaaagcc gcattatttc
     1261 cacaggagga ggcatcatta tcagaccgga gaaccggctg ctactgaaaa aactgggctt
     1321 tgtcgtctgg ctccatacag acgtaaacat cctgtaccag cgtatttcca gatgcaccaa
     1381 ccgccccctg ctccaacaac ccaatcccaa ggccgtgctg gaacatctca tgaaagaacg
     1441 gcatgatttt taccgggaaa cggcgcactt gaccattgat acggccaatc tccacatcca
     1501 tgaaatcgca ttcggcattc tggaatccgc cagagtattc cggtcccggc ggcaataatg
     1561 ccgacccgtt tttccgtgag cgggaaattt tctctttctg acccatccta acgcaagcac
     1621 ttccggggaa gccctttttc ccgctccatg cacaagaaac aacaaaaggc gatccccatt
     1681 aaaggaacgc cttcaggaaa ataataatga ccggaaaagg cttaagcctt acgggcctta
     1741 cggatcattg aattcaccgt ttcagagggc tgtgctccgt tggcaagcca tttgtcaacc
     1801 ttttccagat tgatggtata gttgacgcct tccttcatgg ggtcatagga gcccacttgc
     1861 tcaatgtaac ggccgtcacg acgcgcgcgg ctgtcgacaa cgacgatttt gtagtaagga
     1921 cggtccttgg aaccctgacg gttgagtctg attgctactg ccatatcaat tataaataaa
     1981 aggtgttaat ggaaaacttg cgcgccttgc cactcacatg cgtgcgcgtt cggctttcca
     2041 cagcgggtgc agatgtatgc gatataatgg agtcattgtc aataccagag ttatttttac
     2101 gggaaaaaag cctctcctcc atttctgcat caggaaaaat tttccaccgc catgcggcca
     2161 aaagaataca gcatgctgaa tccgaacccc gccagcacaa gggacaatat tccatccagt
     2221 attccggcat accgtccgta aaaggaacga atgggctgcc agcggaaaac aagagcccac
     2281 aaaatccacc ccaccgttcc cgtcgccacg attaaaaaac cgataagaag agcataggaa
     2341 ggatcatgat ttttttccaa cagcggagcg gaaagagcca caaagaaaaa cgtggccttg
     2401 ggattcatca ggttagtcac cagagcatca cggtaaatac aggaagccgg gggaatatcc
     2461 ccgcacccgg caagaaaagc attcctcccc ctcggccata ttttccatgc caaatacagc
     2521 atccagcagg aagcggcgca aaacagcccc aaccccagga aactgtgaaa aacggatgct
     2581 cctactgtac aagccagtgc agcatgggca actattccag taccaatgcc caaagcagcc
     2641 atcactcccg cgccaaagcc gtaagccagc gtgctccggg taacaaaagc ctggtcgggc
     2701 cccggagatg cctgggaaag aagcagaata cctgcgaata taagttcttc cttcatgaaa
     2761 caaaaaatcc ttatgccctg ggatgcgcct gtctgtaaac ttccgccatt ctggcacgcg
     2821 tcacatgcgt gtaaatctgg gtagtggata aggaagcatg acccagcagt tcctggacgg
     2881 aacggagatc cgccccagcc tccagaatgt gcgtggcgaa tgtatgcctg atcttatggg
     2941 gagaaatggt aaaaggaatg gaagacagct tcacatattt gttcagcatc atctgcacgg
     3001 agcgagcgct taaccgggtt cctatacggg agacaaacaa gggggaattt ttcggcagac
     3061 aggccatgga aacataagtt tccagagcag ccagagcagg agttcccaca ggcaggatgc
     3121 gctccttgcg ccccttcccc attaccttca cgccccggaa acggtggtcc acactgccga
     3181 catccagccc tactaattca ctcaggcgca tcccgcagga ataaaaaagt tccaatatgg
     3241 cggcatcacg gtagggaagc catgcgggag cattggcggg aacggccgtc ttgtagggga
     3301 gctccaacaa ttccaacatc tggttgagtg tcaggaaaac gggaagcgtt ttcttccttc
     3361 tgggaaggga aacgcccgtc atcggattcg tctccagccc gtgcctccgc atcatgaaac
     3421 ggtaaaaact gcgcagggcg gcaaacctca gccggatgga agacgtggcg gcttcatcct
     3481 tcagctcctg gaaaagccag tccctcatct ggtccggcgt acaattctcc cacccaggga
     3541 atgaatcagc cgcccaggcc ctgaactggc gcaaggcgcg ggcgtacacc tccaccgtat
     3601 gaggagaagc ctgcttttcc acctccagat actgaagaaa gccctgttcc ggttccagag
     3661 gaatccccat catgaagaat aaattactcc gtacctttat ccttgtcatc cccggcatcc
     3721 accggcgact ccttcagcag cacttcacta atattggccc ggatgaacaa atcaatatcg
     3781 tccggttctt ccatggcata cagagggctg tctttatggc tgtccgccag agcctgctct
     3841 ctttccaccg ggctgagcac accgtcctgg tccaaatcgt aaagctccat catttccttc
     3901 attttagcct ggtacagagc cgcggcatct ttcatgacgg catgttcctc cggatcaatg
     3961 cggccgttcc cgttggcatc ataattctgc agcaacatgt tacgagtcag cagaaacaat
     4021 ccgggagcaa ccatgaagcg cttccgtcct accgtgcgaa tttccaccat gggcactttc
     4081 cccccctctg cagggggggg gggcggagga atttctttcc tctcggcggg aggataaaac
     4141 ggaattccgt ctctcttgtc cggcccggca tccggcctac gcaccccgcg ccgcttctct
     4201 acatgttccc ggaaagccct gtactcttct ggggaaagcc ttccgtcccc atccttatca
     4261 aacttcctca ggaaggcttt tctggcctcc ctccgcgcag agcgggcatc ctccaccaac
     4321 cgggccttgt cctgttcgga caaaattccc gtacccgtgg tgtcatagcg gtccaaaacc
     4381 atttggcgga tgagcaaagt cacctgaagg cattccagtc cgggcctggg ggcagtttcc
     4441 attttcccgc ggtcgccgaa agccacggat tgccctaccg caaacgggaa cagaccgaac
     4501 gccagcgctg cggaacatga caagataaac atgtggggca atctcataac cacttttact
     4561 aaccccgccg gaaaaagacg gtttcaaaaa taaacatccg cccctgccga cgggcgacac
     4621 gggcggatgc aggaaacgta cggaaagcgg aaaaacttac ttctccactt gaacgatcgc
     4681 gttcttttcc aattcaatca ccacgccttc cgcaatcttg agagaaacgg tgcgctcatt
     4741 caccttctcc acaaaaccgt gcagccctgc gtttgtaatc accttgtccc cgcgttgcag
     4801 ggctgcgatg cgcgcctgct gctccttctg cgccttcctc tggggacgga tcagcatcac
     4861 ccagaacagg acgatgataa tcacaaacat gaacatgggg ctggcgagaa tctgctgaaa
     4921 catatttgcg ggttcctgcc cggcggcccc cgccgcatcc tgagcctgtg ctaacataaa
     4981 gatattcata aaatgtcgtt tgctttatac cgcgcgataa acgaatcctt gaaggaaccg
     5041 aacgtgccgg cggctatggc ctcgcgcgcc tgggccatca gccggagata gaattccaga
     5101 ttctgaaaag aaagcaatct taaagcgagt atttcaccgg ctttaaacaa atgccgcaca
     5161 taagcgcggg aaaattgcgt gacatgcgga tgtccctccg ggtcaattgg gcgggaatcc
     5221 gtcgcccagc gctgattctt gatatgcatg gggccgtccg gagtcagcgc cacgccgtgg
     5281 cgagccaggc gggtaggcat tacgcagtca aacatatcca cgccgcgggc aatcatttcc
     5341 agcaattggg ggggggtgcc cagccccatg gcgtaccggg gcttctcctc cggcagccag
     5401 ggagcggaat gatcaatggc gcggagcatt tcatcctccg gctctcccac ggaaacgccg
     5461 ccgatggcgt acccgtcaaa atccatggcg gccagttctt ccgcgcactt ctttcttaaa
     5521 tccgcataca cggaaccctg cacaatacca aaatggtgct gacgcccttc cccggaacgg
     5581 ggccgatgtt cctgcaccca tgccttgcag cggcgggccc aacgaagcgt gtaccccagg
     5641 gaggcttccg cgtacttcct gtcgcaggga taaggggggc actcgtcaaa cagcatggcg
     5701 atatcactgc ccaggtccgc ctggatctcc atggaccgct ccgggctcag catcatgtac
     5761 gccccgtcca aatggttctg gaaacgcacc ccttcttccg taattttacg gagcttggca
     5821 agggaccaga cctggaaccc gccggaatcc gtcaaaatag ggcggttcca ggaggaaaat
     5881 ttgtgtaatc cgcccatttg cctaattaaa gcggaaccgg ggcgcaggga caaatgatag
     5941 gtatttccca gaataatctc agctcccagc atctccaaat cctgcggatg catggttttc
     6001 acggaaccct gcgttcccac cggcatgaaa atgggggtgg gaacccgtcc atgcggcaac
     6061 tcaagcgaac ccaggcgcgc agccgtggag gaatctttct gacgaagtat aaaaggcatt
     6121 ggaatcagac tttctgcaat ttgataaaac gggcgcggta aatcggcttt cccatggcct
     6181 cccattgaag ctggaaatcc gtacggggat aaaaaaattc gtcctcatcc caactggcac
     6241 ggctaaacag accgctgcgc gccacatggt ccagcaccca cagaaaatat ccttcatgat
     6301 ccgtcttgaa caggaactcc ccgccatcgg acagggaacg gtgaagcacg ggaagaaaat
     6361 cctcctgcac cagccggttc ttatgatgcc tctccttggg ccaaggatcc ggacataaat
     6421 aatgcagccg ggaaatgcag ccgggctgca tcatccattc cagaaagtaa cggctttcca
     6481 cgcgaaacac cttcacgtta tccaacccgc ggacggccgc ccgggaacaa accccccgta
     6541 cgcggcccaa aagccgttct attcccaaaa aacggcgttc cggataatgg gccgccatct
     6601 ggagcaggaa ccctccgtcc ccacacccca aatcaacctc aaacggacca tccgctccaa
     6661 aaatatcaga aggcgccagc accttgaaaa aatcgtctgg aataaaggcc ggatttatca
     6721 tgggaatcaa cgtgcggata agatgcgtta caaaacggac tctgtcaaac gagaagaacc
     6781 cctccccgca aacatggcgg gaagaggccc ggaaaaacgg aaaaaataag cctgaacggc
     6841 ccggagccgt cagctccggc aataaaggcg attataaatt ttacggtcaa aacggtaata
     6901 gcgcgcggcg cggtggggaa ccccctcctc cttctcatcc agcggcacaa tgtaggggcg
     6961 ggaagcgatt tttttgtgga aattacgcac atccatgggc ttgccgtgaa tctcctcatt
     7021 cagccgccgg agctgaagag ccgtaaactt ggcaggcaac atgtcataaa tcagcgcagg
     7081 ctccttcttc acggaagaac gcactctctc cagcgccagg tcaataatat cccggtgatc
     7141 aaacgccagt tccggcaaat catctacagg aacccagcgg gtcttatggc tttccatcag
     7201 tttgcgcagc ttggtggaaa tcctgagcat ggccatgtag gccaccgtca ccaggcgtcc
     7261 tatcttctgg ccggaaacag cctccaccca tgccctgtcc tccggattct tgatgcgcga
     7321 tggagcgccg aaagtatgga actgttccaa atggggggaa gacatgccgg tcatgtcaaa
     7381 caaaacgcgg cgggcagcgg catccaattc ttcctcctca taaatcaaat cgccgggcaa
     7441 tttggaaatg gcgccggtat ggccgggttc aaattgcgtc ttctcaacca gaaggacttt
     7501 cagaccttct tcatcgaacc cgatcaacac acaatccact gagagatgcg gataaaatgc
     7561 ctgcctgctc attatgttat ctattattgt agtaagttcc cagaatcgca tcaacgctac
     7621 gcaagcgaac cagccaagtc aaccaaaatc aaaatagaaa cggcaatgaa aaaccgttcc
     7681 ccttgccccc gggaaacatc acgccacttc cggaatactg cttctcacgg gcggaaaaac
     7741 gcgcctgcgt cttgcatt
//
LOCUS       DAOMSU010000086         7707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_731_length_7707_cov_2.516858, whole genome shotgun sequence.
ACCESSION   DAOMSU010000086 DAOMSU010000000
VERSION     DAOMSU010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_731_length_7707_cov_2.516858"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="PK077_05155"
     CDS             <1..205
                     /locus_tag="PK077_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23721.1"
                     /translation="KSPFPGESQNAQPPPLRQTKKEACGRQKKSRKTGEIFLENMTNN
                     TIYSVYKKILYNISFSSGLFFSR"
     gene            286..3828
                     /locus_tag="PK077_05160"
     CDS             286..3828
                     /locus_tag="PK077_05160"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRN23722.1"
                     /translation="MTTDNSQTILKLRTGQEIILPQRKDLLGGLKFTRRFSHNEVHPY
                     DEVDWTRRDVRIMDWKTGKIIYERLGLEAPAHWDDNAVKITADKYLFGSEPGSLEYED
                     SFRNIYDRISNTYTVWGWEEGYFATLEDAEIFNEEIKAMLVQQIWAPNSPVWFNIGHW
                     EQWRWGRPDLRENYTGHGNKAYHTKGTKNNLKTFMVQSTYEYPQCSACFLTEVGDSME
                     DILDHLTTEGRIFASGSGVGINLSTLRSSKEPISGKGRSSGPISFDRGWDRMAGAIKS
                     GGKTRRAARMVLMFSDHPDIFEFINTKNRQEDIAKVILREHNVHVELKQIAETKLVAG
                     TPAEKAAARVILSLPLATRNSFDPHMDALLYGETLSHQNANHSVSLKGDFWQALANNG
                     NTYTRWVTNPAHIEQTFRAQELLEAMAQSIWDNGEPGVHNNDVINLWNPVKSIGTITT
                     SNPCSEYVFLNNTSCNLSSFNAYRFLIKGEDGKPVFDADALTHAARLAMVCADLNVER
                     GGFPIEEIAEGTYKYRTTGIGFANVGGSLMALGVPYDSDEGRWIASQLCSALTAACWT
                     ASAEMGAELGSYVEYPASKKDLLAVLRLHNAAQKLASALPSQKDSKTLDDLIENIIAN
                     AGGILPEAQGLTASYALHAYLKSFKAPTMMNRERIAPAAKLAEAASDMWAKVIKATAH
                     RNAFVSVMAPTGTISAPLGCYDEGTTSIEPDYTLVKWKQLAGGGSLKMFNRLALEGLR
                     ALGYPEDFVNEAALEVAGLDGLISAFQGNMDSVVNQLIVDPCNDQAGPVRLAWRRLLS
                     GTESRSEIQEKVAYISNPANMAVLTADELAVVNGAAHIESIPWLNKKDLPVFDCAATN
                     GNGIRAISPAGHVLMLGALQPFISGACSKTVNMPVSAGVQDIYDSLIMSHELGVKCIA
                     IFRAGSKANAVYVVDTPETRMFKANHVWEQLVNAGSDAIDEIIAEASKPRQRKLPGRR
                     LGQTVKFSVGGQLTGYLTVGVYADGTCGEVFGRLGQVGSFASGMFEAYCKLLSTALQF
                     GVPLKEVVKGFRNYSFEPSGFCRVGDDTEADTCTEIRSCASVVDLIAKILAWLFPESN
                     GYRLRDVFSIPSVSLPGQAPDTTVSVLPPRIITAPAHPATPALPEIPAGKVPEGTMLN
                     GASLCPQCHSLAYVQDGKCKSCRSCGYKDGGCGE"
     gene            4094..5026
                     /locus_tag="PK077_05165"
     CDS             4094..5026
                     /locus_tag="PK077_05165"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HRN23723.1"
                     /translation="MKPYYTLADLLDEGHPFDAGESVPAKLAVIGFPIAHSKSPAMQQ
                     AALDAAGIRARYIRIQASPEEFGEVVRLLRERGFTGANVTVPHKQAACSLCNDTDALS
                     RVTGSVNTLVFQKDGSVSGFNTDGPGFARAIREEFSVDLRDLKVALLGSCGGAGLALA
                     YTCAMQRCERLTLAGRSEEKLQELKNRLSSFFIDEHRLEGASDRLAAHQNNTPRFNAA
                     VEDADLIVNATSLGLKPTDPSPVPPALLSAHHLVYDLQTHDDAFQMEARFQGARVSNG
                     LSMLVHQGALSFERWFGVKPDISAMRRALEQKHD"
     gene            5019..6044
                     /locus_tag="PK077_05170"
     CDS             5019..6044
                     /locus_tag="PK077_05170"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000701 - purine-specific mismatch base
                     pair DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRN23724.1"
                     /translation="MTDAPPHFNVHAFRNALVEWFRREGKDYPWRRTTDPWHILVSEL
                     MLQQTTIPTVLGRYDRWMRQFPTPGHLAAVDEQTALRSWEGLGYYRRVRSLQAIAREI
                     VNKFGGRFPDNAEGLKRLPGIGPYTSGALLSFAFNKAAPIVDANVARVLARIDNYSVP
                     VDSTEGQKYLWSRAESLVDPEHAREFNSAIMELGQTCCSISSPDCLLCPVRPFCSAER
                     PETLPVKNPKPQVTRVEHHDILYIRGKSVLLAKCPEGKRHAGMYRFPQREDDHTLSLP
                     HVLKQTYSITRYRVTRYIHHVTDTPLLREGEEFVPLDKIHGLPMASPDRKALNSPALG
                     KLLNHIR"
     gene            6041..6619
                     /gene="aat"
                     /locus_tag="PK077_05175"
     CDS             6041..6619
                     /gene="aat"
                     /locus_tag="PK077_05175"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HRN23725.1"
                     /translation="MNDRLTPELVLSAYCQGCFPMADPETGEISFYEPDPRALIPLDD
                     RFHIPHGLKRALNKKPFELRMDTAFPEVVHACARTEQPEEQWIDGQIEEAYGKLHEMG
                     FAHSVECWDEEGLQGGLYGVALGKAFFGESMFHRKTDASKIALVALVQYLRAHQFLFL
                     DTQWTTPHLLKFGTYEVPAEEYRKMLKRALAG"
     gene            complement(6998..>7707)
                     /locus_tag="PK077_05180"
     CDS             complement(6998..>7707)
                     /locus_tag="PK077_05180"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421001.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRN23726.1"
                     /translation="QKIGMPVEVIMRRVSELHEFNPMLGHRGCRLGIAYPEITEMQAR
                     AIFEAAVEVSQEEGFAVVPEIMVPLVAFKKELDIQKAIIDKVAQQVFEEKGAKVDYLV
                     GTMIEIPRAAVTADEIAETAQFFSFGTNDLTQTGLGMSDDSGSFLPQYQELEIIKKNV
                     FASIDQNGVGKLMKIAVELGRSTRPDIKLGICGEHGGDPASVKFCNTLGLTYVSCSPY
                     RVPTARLAAAQAALEQE"
BASE COUNT         1685 a         2412 c         2131 g         1479 t
ORIGIN      
        1 gaaaagcccg tttccggggg agagccaaaa cgcccagccg cccccccttc gccaaacaaa
       61 aaaagaagca tgcggaagac agaaaaaatc acgcaagaca ggagaaattt ttctagaaaa
      121 catgacaaac aacacaatat atagcgtata taaaaaaata ctgtacaata tttccttttc
      181 atcaggatta tttttcagta gataatgcac acgctacgcg ccgccgcccc acgacgaagc
      241 gcaagcctac gggattattc ccaaaccttt ctacttcgca atccaatgac cacagacaac
      301 tcgcaaacca tactcaaact gcgcacaggc caggaaatca tcctccccca gaggaaagac
      361 ctgctgggcg gtttgaaatt caccagaaga ttttcacaca atgaagtcca cccctatgat
      421 gaagtggact ggacgcgccg cgacgtccgc attatggatt ggaaaaccgg caagatcatt
      481 tacgagcgcc tgggtctgga agctccggcc cactgggatg acaacgccgt caaaatcacg
      541 gcggacaagt accttttcgg cagcgaaccc ggctccctgg aatatgaaga cagcttccgc
      601 aatatctacg accgcatttc caatacctat accgtatggg gatgggagga aggctacttc
      661 gccacgctgg aggacgcgga aattttcaat gaggaaatca aggccatgct ggtgcagcag
      721 atatgggcgc ccaactcccc cgtctggttc aacatcggcc actgggagca gtggcgctgg
      781 ggacgcccgg acctgaggga aaactataca ggccacggca acaaggccta ccataccaaa
      841 gggaccaaga acaatctgaa aaccttcatg gtccagtcta cgtatgaata cccccagtgt
      901 tccgcctgct tcctgacgga agtgggagac agcatggagg atattctgga ccacctgacg
      961 acggaaggcc gtattttcgc gtccggttcc ggcgtgggca tcaacctgtc caccctccgg
     1021 tcctccaagg agcccatcag cggcaagggc cggtcctccg gccccatctc cttcgaccgc
     1081 ggctgggacc gcatggccgg cgccatcaaa tccggaggca agacgcgccg cgccgcacgc
     1141 atggtcctga tgttcagcga ccatccggat attttcgaat tcatcaatac gaagaaccgc
     1201 caggaagaca tcgccaaggt tatcctgcgc gaacacaacg tgcacgtgga gctcaagcag
     1261 attgcggaga ccaagctggt tgccggcact ccggcggaaa aggccgccgc ccgcgtcatt
     1321 ctttccctgc ccctggccac ccggaacagt tttgacccgc acatggatgc cctgctgtac
     1381 ggggaaacgc tctcccacca gaacgcgaac cattccgttt ccctgaaggg ggatttctgg
     1441 caggcgctcg ccaataacgg caatacctac acgcgctggg tcacgaatcc ggcccacatc
     1501 gaacagacat tccgcgccca ggaacttctg gaagccatgg cccagtccat ctgggacaac
     1561 ggggaacccg gcgtgcacaa taacgacgta atcaacctgt ggaaccccgt caagtccatc
     1621 ggcaccatca ctacgtccaa tccctgctcg gaatacgtct tcctgaacaa tacgagctgc
     1681 aacctttcct ccttcaacgc ctaccgcttc ctgatcaagg gagaggacgg caagcccgtt
     1741 tttgatgcgg acgccctgac ccacgccgcc cgcctggcca tggtctgcgc ggacctgaat
     1801 gtggaacgcg gcggcttccc gattgaagaa atcgcggagg gcacctacaa gtaccgcacg
     1861 acaggcatcg gcttcgccaa cgtgggcggc tctctgatgg ccctgggcgt gccttacgat
     1921 tccgacgaag gccgctggat tgcctcccag ctttgcagcg ccctgacggc ggcctgctgg
     1981 acggcctccg cggaaatggg cgcggaactg ggctcctacg tggaataccc cgccagcaag
     2041 aaagacctgc tcgccgtgct gcgcctgcac aacgcagccc agaaactggc aagcgccctg
     2101 ccctcccaga aggattccaa gacgctggac gacctgattg agaacatcat cgccaatgcc
     2161 ggagggatcc tgccggaagc gcaggggctc accgcctcct atgccctgca tgcctacctc
     2221 aagagcttca aggcccctac catgatgaac agggagcgca tcgcccccgc ggccaaactg
     2281 gcggaagccg cttccgacat gtgggcgaag gtgatcaagg ccacagcgca ccgcaacgcg
     2341 tttgtttccg taatggcccc cacgggcacc atttccgccc cgcttggctg ctatgatgaa
     2401 ggcaccactt ccattgaacc ggactacacg ctggtgaaat ggaagcagct ggcgggcgga
     2461 ggctccctga agatgttcaa ccgcctggcc ctggaaggcc tccgcgctct gggttacccg
     2521 gaagacttcg tcaatgaagc cgctctggaa gtggcgggtc tggacggcct gatttccgcg
     2581 ttccagggga atatggattc cgtcgtgaac cagctcattg tggacccctg caatgaccag
     2641 gccggccccg tgcgccttgc ttggcgccgc ctgctctccg gcacggaatc ccgcagtgaa
     2701 attcaggaaa aagtggccta tatcagcaac ccggccaaca tggccgtgct gacggcggac
     2761 gaactagccg tagtgaacgg ggccgcccat attgaatcca ttccgtggct gaataagaag
     2821 gatctgccgg tctttgactg cgccgccacc aacggcaacg gcatccgcgc catttccccc
     2881 gccggccatg tgctgatgct gggcgccctc cagcccttca tctccggcgc gtgctccaag
     2941 acggtcaata tgcccgtctc cgccggcgtg caggatattt acgactccct catcatgtcc
     3001 cacgaactgg gcgtgaagtg catcgccatc ttccgcgccg gctccaaggc gaatgccgtt
     3061 tacgtggtgg ataccccgga aacgcgcatg ttcaaggcca accacgtctg ggaacagctg
     3121 gtgaatgccg gctcagacgc cattgacgaa atcatcgcgg aggcatccaa gccgcgccag
     3181 cgcaagctgc ccggccgccg cctgggccag accgtcaaat tctccgtggg aggccagctg
     3241 acgggctacc tgacggtagg cgtctatgcg gacggcacct gcggggaagt tttcggacgc
     3301 ctgggacaag tcggctcctt cgcctccggc atgtttgaag cttactgcaa gctcctctcc
     3361 accgcccttc agttcggcgt gcctctcaag gaagtggtca agggtttccg caattactcc
     3421 ttcgagccct ccgggttctg ccgcgtgggg gatgatacgg aggcggacac ctgcacggaa
     3481 atccgctcct gcgcttccgt ggtggattta attgccaaga ttctggcgtg gcttttcccg
     3541 gaaagcaacg gctaccgcct gcgggacgtg ttctccatcc ccagcgtcag ccttccgggc
     3601 caggctccgg atacgacggt aagcgtgctg cccccccgca tcatcaccgc tccggcgcat
     3661 cccgctactc cggccctgcc ggaaattccc gccggaaagg ttccggaagg aacgatgctc
     3721 aacggcgcct ccctctgccc gcagtgccac tccctggcct atgtccagga cggcaaatgc
     3781 aaatcctgcc gctcctgcgg atacaaggac ggcggctgcg gggaataaac ggtaaccaga
     3841 tcattccaga cccgctcttt ccgggcggaa tccggctgct gccgggttcc gcccggtgaa
     3901 tttacggaaa aggtattttt tccgggagag acgattttcc ggtcttcccg gcccccgtcc
     3961 agcatgaact ttccggaaaa agcgaacgga aaccgccgcg cggggaaaag gaatgatagg
     4021 cgtttgcctt gaaatccggc agcatttcat tcatagtccg ccgcatacgc ctgaagccgc
     4081 acctttattt tccatgaaac cctactatac cctggctgac cttctggatg aaggacatcc
     4141 gtttgacgcc ggggaatccg ttcccgccaa gctggccgta atcggtttcc ccattgccca
     4201 ttccaaatct cccgccatgc agcaggccgc gctggacgcg gcgggcattc gcgcccgtta
     4261 tatccgtata caggcttctc cggaagaatt cggggaggtg gtgcggctcc tcagggaaag
     4321 gggattcacg ggggccaacg tgacggtgcc ccacaagcag gctgcctgtt ccctgtgcaa
     4381 cgacacggac gctctttccc gcgtcaccgg ttccgtcaat acgctcgtct ttcaaaagga
     4441 cggttccgtc tccggcttca acacggacgg cccgggtttc gcccgcgcca tccgggaaga
     4501 attttccgtg gatttgcgcg atttgaaagt ggcccttctg ggttcctgcg gcggagccgg
     4561 gctagccttg gcctacacct gcgccatgca gcgctgtgaa cggctgacgc tggccggaag
     4621 atcggaagaa aagcttcagg agcttaaaaa caggctgagt tcctttttca ttgacgaaca
     4681 caggctggaa ggagcctccg accgcctggc ggcccaccag aacaatactc cccgtttcaa
     4741 tgccgccgtg gaggatgcgg acctgattgt caacgccacc tccctcggcc tgaaacctac
     4801 ggacccttcc cccgtgcctc ccgcactgct ttccgcccac catctggttt acgatttaca
     4861 gacgcatgac gatgccttcc agatggaagc ccgctttcag ggcgccagag tttccaacgg
     4921 cctttccatg ctggttcacc agggggccct ctcctttgaa cgctggttcg gcgtcaaacc
     4981 ggatatttcc gccatgcgcc gggctcttga acaaaaacat gactgacgcc cccccccatt
     5041 ttaacgtcca tgctttccgg aacgcgctgg tagaatggtt caggcgcgaa gggaaagact
     5101 acccgtggag gcggacaacg gatccgtggc acatcctggt ttccgagctg atgctgcagc
     5161 agacgaccat tcccaccgtt ctgggaagat atgacagatg gatgcgccag ttccccaccc
     5221 cgggccatct ggccgccgtg gacgagcaga cggccctgcg ctcctgggaa ggactgggct
     5281 attaccgccg cgtgcgctcc ctccaggcca tcgccaggga aatcgtcaac aagttcggag
     5341 gacggttccc ggacaacgcg gaagggttga aacgcctgcc gggcatcggc ccctacacgt
     5401 cgggagcgct cctctccttc gccttcaaca aggcggctcc cattgtagac gccaatgtcg
     5461 cgcgcgtcct ggcccgcatt gacaattatt ccgttcccgt ggattccacg gaaggccaaa
     5521 aatacctgtg gagccgcgcg gaaagcctgg tggacccgga acatgcccgt gaattcaatt
     5581 cagccatcat ggaactgggg caaacctgct gcagcatcag ttctccggac tgcctgctgt
     5641 gccccgtgcg ccccttctgc tccgcggaac ggccggaaac gcttcccgtc aaaaatccca
     5701 agccgcaagt cacccgggtg gagcaccacg acattcttta catccgcggc aaatccgtcc
     5761 tgctggccaa atgcccggaa ggcaagcgtc atgccggcat gtaccgcttc ccccagaggg
     5821 aggacgacca caccctttcc ctgccccatg tcctgaaaca gacctacagc attacccgct
     5881 acagggtgac ccgctacatc caccatgtga cggatacgcc tctcctcagg gaaggagagg
     5941 aattcgtgcc gctggacaaa atccacgggc tgcccatggc atctccggac cgcaaggcgc
     6001 tgaactcccc cgccctgggc aaactgctga accatatcag atgaatgaca gattaacgcc
     6061 ggaacttgtc ctctccgcct attgccaggg gtgttttccc atggcggacc cggaaacggg
     6121 agaaatctcc ttttacgaac cggacccgcg cgccctgatt ccgctggacg accgcttcca
     6181 catccctcac ggcctcaaac gggctttgaa taaaaaacct tttgaactcc ggatggatac
     6241 cgcttttccg gaggtggtgc atgcctgcgc ccggacggag cagccggaag aacaatggat
     6301 agacgggcag attgaagaag catacggcaa gcttcacgag atgggcttcg cccacagtgt
     6361 ggaatgctgg gacgaggaag ggctgcaggg aggcctgtac ggagtagcct tggggaaggc
     6421 ttttttcggg gaaagcatgt tccaccggaa aacggacgcc agcaagattg ccctggtggc
     6481 cctggtgcaa tacctgcggg cgcaccagtt ccttttcctg gatacccagt ggactacgcc
     6541 gcatctgctg aaattcggaa cctatgaagt gccggcggaa gaataccgga aaatgctgaa
     6601 gcgagcgctg gcaggataat aaaccatgtt ttcattgtcc ggaataaaca gccggaatgg
     6661 attctatggc gtttattccc aactatggaa aatccggcag cgcaccggat tgcaagaact
     6721 gttggtcatt tttcttcagc ctttaccatc aatacttgaa ccatcaagcc gaaccctatg
     6781 ctcgaatatc cagaaagcat ggttccggca taaaaaacgc cggaattgaa attggcggcc
     6841 tccgggaaag gcgagaactg tgaaggtatg tccacccgcg cgcatggaac aatcggcccg
     6901 caggttcaag ccatatccgc caaggctatt cccatccgct aaaaagcccg tggttccgaa
     6961 gaaccacggg ctgtctatta acccttaaca aaaaggttta ttcctgttcc agagccgcct
     7021 gggccgcagc cagacgggcg gtcgggacgc ggtagggaga gcagctcacg taagtcaggc
     7081 ccagcgtgtt gcagaacttg acggaagcgg ggtcgccgcc gtgttcgccg cagatgccca
     7141 gcttgatgtc cgggcgggtg gagcggccga gttccacggc aatcttcatc agcttgccga
     7201 cgccgttctg gtcaatggaa gcgaagacgt tcttcttgat gatttccagt tcctgatact
     7261 gcggcaggaa ggaaccggag tcgtcgctca tgcccaggcc cgtctgggtc aggtcattgg
     7321 tgccgaagga gaagaactgg gcggtttccg cgatttcgtc agccgtgacg gcagcgcgcg
     7381 ggatttcaat catggtgccg acgaggtagt ccactttggc gcctttttcc tcaaatacct
     7441 gctgggccac cttgtcaatg atggctttct ggatgtccag ttccttcttg aaggccacca
     7501 gaggaaccat gatttcaggc accacggcaa atccttcttc ttgggagact tccacagcgg
     7561 cttcaaagat ggcgcgggcc tgcatttccg tgatttccgg ataggcgatg ccgaggcggc
     7621 agccgcggtg gccgagcatc gggttgaact catggagttc ggacacgcgg cgcatgatga
     7681 cttccacagg catgccgatt ttctggg
//
LOCUS       DAOMSU010000087         7683 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_734_length_7683_cov_2.913739, whole genome shotgun sequence.
ACCESSION   DAOMSU010000087 DAOMSU010000000
VERSION     DAOMSU010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7683)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_734_length_7683_cov_2.913739"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3298
                     /gene="hrpA"
                     /locus_tag="PK077_05185"
     CDS             <1..3298
                     /gene="hrpA"
                     /locus_tag="PK077_05185"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HRN23727.1"
                     /translation="SLNIDFLLGYLRLLLGRRRDLRLVISSATLDAGGFSEFFGGAPI
                     IQVEGRTYPVDLHYLPPLHDDEELPAHVARAVDWLDTLDDRGDVLVFLPGEREIREVA
                     EKLKGRNLRNTCILPLFARLGLADQQRIFHPEQGYRRIVLATNVAETSLTIPGIIYVI
                     DSGLARVSRYSPARQVQRLQIEPVSKASARQRAGRCGRVCEGVCIRLYSEKDWEDRPE
                     FTDPEIRRSALAGALLRMKDLGLPEMPEFPLPDPPSSKLVTEGYRTLREIGALDKARQ
                     LTPVGRKLARLPIDPRLARMLLEAQHEQALPEMLVIVAGLGIMDPRERPADAAQKADQ
                     AHAQWKEEDSDFLSLLKLWKAAWQFREGRRWRKNQLRKWCGKNFLNFNRMMEWFNLWE
                     DLSRLVRETLKCKISPLEEKTERQASFAMIHRSILAGVPRQFGLWDADNREYRGAGGR
                     SFGIFPGSGLFRRKKRSEWVMGVELVDTTRLWMRRASILEPEWVERVAPHLCESHYSG
                     ARWDREQGAVYAVERVVCGGLRIIDNRRVHYGRINPAHAREIMIREGLLGGGFRTNPA
                     CIRHLETLREDVRQIELKLRRPDQVWCEEGVFEFFNRLVPPDCCTARAFLRWAAGVEK
                     KNPEALHVPPQEAMYEFWGKDLLDGFPDEITCKGAEYAVYYQNDPGAEDDGVTLGVHI
                     DQLPDIPEWLPEWGVPGHLAQRAECLLRSLPKDLRVFLQPINQKAAYFAELRHGLEPD
                     GPLAGKLAEFVEVETGKFCAPSFFDMNRLPAELVTKIWVCDDEGEELAMGTDVAELNN
                     RLGKKLSRRFQETAEDIVSVTGMKEWSCGDLEYTVDVAGRPGYVALVDEGASVGVRVF
                     EDELSAAESHREGCLRFMRLRQTDQLNHLRRKFPLKLEGKLSLHMLGREPSTNAENLV
                     DVAAEIAMGRPLPRTAEQFAAAEMNLRQNLFDAAHNVADIWELVAGTEHAVRDFTALQ
                     KGVRHTERITADLQRQLDWLLRPRFLWAHGAERLPDLKRYMQGIAERIRRIGQQPLAR
                     ELERLDLFERVYFPWHQVLKEHAGDLRWMQYGYLLEEYRLAIFAPAISVKGRVSEKRL
                     VASFEALSSQ"
     gene            complement(3391..4098)
                     /locus_tag="PK077_05190"
     CDS             complement(3391..4098)
                     /locus_tag="PK077_05190"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRN23728.1"
                     /translation="MMRYTVIGLGQFGQELCTELSARNIEVVAVASTDEELEQIKDLV
                     TYAVVTDYTNPAALCELELDDQSAVIVAIGDSFEENLLVVTHLQKMGVRYIYARVMSP
                     VHEHILSQMNVYALINLSRVASKQLASQLESPEFLRVSPMDENHSIVEIAVPRIWVGK
                     RLRDVGLRTEHHLNLLTIRRGEAQTPQGRREILSIPRIPVIGTPSPDLVFEDHDILVL
                     FGKETNLIEFAQLSKGL"
     gene            complement(4106..5854)
                     /locus_tag="PK077_05195"
     CDS             complement(4106..5854)
                     /locus_tag="PK077_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRN23729.1"
                     /translation="MTTTRSGFTKLAAVCIAILLGAVVWELGWPLTRAEHEWSRLIAA
                     VATLGHLTGILGSYFRKEINRPSLRLLIFQIISCLMIFLLIAREQQEAGYMRFTELSR
                     LVITAGLIAIPTLMSLTRIFEWLVGKQKKGRPLMAPAMQFVASLGVVILAGTGLLLLP
                     NSTYPGITLSFTDALFTSTSAVCVTGLNAVDFAHTFTPLGEMFTLALIQIGGFGIMTF
                     AYFVAMVAGQGFSLRDRVLLTDLLDEDNLGAVVSFITTIVVSTLFIELCGAVLLYFSW
                     EGKDINLMGEPLWWHSLFHSVSAFCNAGFSTFPMNLMEPGIRLCYSGQAVIMALIVCG
                     GLGFGIYKEINSRLANRFFSKHRRLHMQWTPYFKLVMISTGILLAGGALAIFAVSSPH
                     AAEPLGEHLWICLFDSVTARTAGFNISDYSRYLPAASLIMCGLMMVGGSPGGTAGGMR
                     TTTCAIAGAEILRILRGRDHVEFFRRRIDQRTVARCVITVVVSCAWIGCFTVLICALE
                     PSMSSLDIFFENCSAFATVGVSRGITPDLSDPSKYLLIVNMLAGRVGLFAFLIALAGT
                     PTPRHYRYPSVKIPLT"
     gene            complement(6033..>7683)
                     /locus_tag="PK077_05200"
     CDS             complement(6033..>7683)
                     /locus_tag="PK077_05200"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF262 domain-containing HNH endonuclease family
                     protein"
                     /protein_id="HRN23730.1"
                     /translation="EFSIERTTSKSAKTYFLGSIVSFENDQGEKEIIDGQQRITSLFL
                     LLRAVYTKLESEENKTKEIKNFISKIQPALWLEDELTGEVDKTKTLLRSEVATDQGNE
                     ILRRILETGQTEKDAKDNYSHNFNKFVELYENKAVDNAMHMFHFINALLNYSILLPIS
                     ADNQDTALTIFSTLNNRGLPLSDADIFKSLIYKKLTAGEKKSFAHKWKELEAEASKHG
                     ETIQSLFNYHMFYLRACDGDVKTTTSGVRKYFTEKSKNRLKPEIIDTLADSLNLWKVI
                     RSGEILANESWSENKDILKALDILVAYNNEFWKYPTIIYYNQHKDDPEFEGLFLKFLR
                     KLCVLLVTRYLESPSISAVKGTILKLNAAIIKTPEPEFVAGFKADTSEEDAISLIVTP
                     PRTMVRMLLKILAYDNQNQSELLPDKWEIEHIFPQKWDTSFFTLDSDETNKTLEHIGN
                     KIPLEKPMNIRASNNYFDRKKEQYSKSAIAITQDFGKSNRVNWIPTDIKLRDKEITDQ
                     LTRLFAKWVDEYEPTPVPEVKPVPTPEQLEQIRQLRELGFI"
BASE COUNT         1785 a         1873 c         2207 g         1818 t
ORIGIN      
        1 cagcctcaac attgattttc tgcttggcta tctgcgtctt ttgctgggca ggcggagaga
       61 cttgcggctg gtcatcagtt ccgcgacgct ggatgccggt gggttttccg aattctttgg
      121 aggcgcgcct attattcaag tagaaggccg cacttatccg gtggatctcc actatctgcc
      181 gccgctgcat gacgatgagg aactcccggc tcatgtggcg cgtgccgtgg attggctgga
      241 tactctggac gatcgcgggg atgtgcttgt cttccttccc ggagaacgcg aaatacgtga
      301 ggtggcggaa aaactgaaag gccggaatct gagaaatacg tgcattctgc ctttgttcgc
      361 caggctgggc ctggcggacc agcaaaggat ttttcatccg gagcagggat accgccgcat
      421 cgtgctggct acaaatgtgg cggaaacgtc cctgacaatc cccggcatca tttatgttat
      481 tgactccggc ctggcgcgcg tcagccgcta tagcccggca cgtcaagtgc agcggcttca
      541 gatagaaccc gtctccaagg ccagtgcccg ccagcgcgct ggccgttgcg gccgtgtgtg
      601 cgaaggtgtg tgcatccgcc tgtacagtga gaaggactgg gaggacaggc cggaatttac
      661 ggatccggaa atcaggcgca gcgcactggc cggggcgctg ctccggatga aagacctagg
      721 attgccagaa atgccggagt tcccacttcc ggatcctcct tcatccaaac tggtgacgga
      781 aggttaccgc accttacggg agatcggcgc tctggacaaa gcccggcaac tcacgcccgt
      841 aggcagaaag ctggcgcggt tgcccattga cccgcggctg gcccgcatgt tgctggaggc
      901 tcaacatgaa caggccctgc cggaaatgct ggtgattgta gccggcctgg gcatcatgga
      961 tccccgtgaa cgccctgcgg atgccgctca gaaagcggac caggcccacg cgcagtggaa
     1021 ggaagaagac agtgacttcc tgtctctgct taaattatgg aaggctgcat ggcaattccg
     1081 ggagggaagg cgctggcgca aaaaccagct tcggaaatgg tgcgggaaaa atttcctcaa
     1141 tttcaaccgc atgatggaat ggttcaattt atgggaggat ctttcccggt tggtcaggga
     1201 aacactgaaa tgcaaaattt cccctttgga agaaaaaacg gagcggcagg cttcctttgc
     1261 catgattcac cggagcatcc tggcgggcgt tccgcgccag ttcggcctct gggatgcaga
     1321 caaccgggaa tatcgcggcg ccggaggacg ttccttcggc atttttcccg gttccggcct
     1381 gttccgccgt aaaaaaagga gtgaatgggt aatgggcgtg gagttggtgg acaccacgcg
     1441 cctgtggatg cgccgcgcct ccattttgga accggaatgg gtggagagag tagcgcccca
     1501 tctgtgcgaa tcacactatt ccggagcgcg gtgggacagg gagcaggggg ctgtttacgc
     1561 agtggaacgc gtcgtgtgcg gcggcttgcg catcattgat aacaggcgtg tgcactatgg
     1621 ccgtatcaat cccgcccatg cgcgggaaat aatgatacgt gaaggcctgt tgggcggtgg
     1681 tttccgcacg aaccccgcct gtatcaggca tctggaaacg ctccgggaag atgttcggca
     1741 gatagagctg aagttgcgcc gtccggacca ggtctggtgt gaggaaggag tttttgaatt
     1801 ctttaacagg cttgttcccc cggactgctg tacggccagg gcctttttgc ggtgggccgc
     1861 cggtgtggag aagaaaaatc cggaagctct gcatgttccg ccacaggaag ccatgtacga
     1921 attctggggg aaagacctgc tggatggttt tccggatgaa atcacctgta aaggagcgga
     1981 atatgccgtg tattaccaga atgatcccgg cgcggaagat gacggcgtga ccctgggcgt
     2041 ccacatcgac cagttgccgg atattcccga atggttgccg gagtgggggg ttcccggcca
     2101 tctggcccag cgggcggaat gcctgctgcg ttcccttccc aaggatttgc gcgtctttct
     2161 ccagcctatc aaccaaaagg ccgcttactt tgcggaactg aggcatggcc tggaaccgga
     2221 cgggccgctg gcggggaaac tggcggaatt tgtggaagtg gaaacgggaa aattttgcgc
     2281 gccctccttt tttgatatga accgtcttcc tgcggagctg gttacgaaaa tatgggtgtg
     2341 cgatgatgaa ggggaagagc ttgccatggg cacggatgtt gcggaactga ataaccgcct
     2401 gggtaaaaaa ctttcccgcc ggttccagga aacagcggag gatatcgtgt ccgtaaccgg
     2461 catgaaggaa tggagctgcg gagatttgga gtacacggtg gatgtggcgg gaaggccggg
     2521 ttatgtggct ctggtggatg aaggagcatc cgtgggcgtc cgggtctttg aggatgaact
     2581 gagcgctgcg gaatcccacc gggaagggtg cctgcgtttc atgcgcctgc gccagacgga
     2641 ccagcttaac catctccgca gaaaatttcc gttgaagctg gaaggaaagc tctctcttca
     2701 tatgctcgga cgggagccgt ctaccaatgc ggaaaacctg gtggacgttg ccgcggagat
     2761 agccatgggc cgtcctttgc cgcgcacggc ggagcaattc gctgctgcgg aaatgaatct
     2821 gcgccaaaat ctttttgatg cggcgcataa cgtggcggat atatgggaac tggtagcggg
     2881 tacggaacat gccgtcaggg actttacggc gctgcaaaag ggagtgcgcc atacggagcg
     2941 catcacggct gaccttcagc gccagcttga ctggttgtta cgcccccgtt tcctgtgggc
     3001 gcatggagcg gaacgcttgc cggatctgaa acggtacatg cagggaattg cggagcgtat
     3061 cagacgcatc ggccagcaac cccttgccag ggaattggaa cgcctggatc tctttgaacg
     3121 cgtgtatttt ccctggcatc aggttttgaa ggaacatgcc ggagacctgc gctggatgca
     3181 atacggttat ttgctggaag aataccgcct ggccattttc gctcccgcca tctccgtcaa
     3241 gggccgcgtc tccgaaaaac gtctggtggc ttcttttgag gcattgtcaa gccagtaaaa
     3301 ccaggacgga ggctgtttgc acaggcggct tatccgtcgc ctttttctga aatggagagg
     3361 gataccctca gcctgcttgc ggaggatggt ttacaatccc ttggataatt gggcgaattc
     3421 aatcaaattg gtttccttgc cgaacaggac cagaatgtca tggtcttcaa aaacgagatc
     3481 cggggaagga gtgccaatga cgggaatgcg cggtatggac aggatttccc ggcgcccctg
     3541 cggagtctgg gcctccccgc ggcggatggt gagcaggttg aggtggtgtt ccgtgcgcag
     3601 gcccacatcc cgcaggcgct tgcccaccca gatgcgcggc acggcaattt ccacgatgct
     3661 gtggttttcg tccatggggg agacgcgcag gaattccggg gattccaact ggctggcgag
     3721 ctgcttggaa gctacgcggg ataagttgat gagcgcatat acgttcatct ggcttaaaat
     3781 gtgttcgtgc accggactca tgacgcgggc gtagatgtag cggacgccca ttttctgaag
     3841 gtgcgtgacg acgagaaggt tttcttcaaa actgtctccg atggcgacaa tgacggcgga
     3901 ctggtcgtcc agttccaact cacacaaagc ggccggatta gtgtaatccg tcaccacggc
     3961 ataagtcacc agatccttga tttgctccaa ttcttcatct gtggaggcaa cggctactac
     4021 ttcaatatta cgggcggaaa gttctgtgca gagttcctgt ccaaactggc ccaggccgat
     4081 aacggtgtat ctcatcatgg tatattcagg ttaacgggat tttgacggat ggatagcggt
     4141 aatgtctcgg agtaggggtg ccggccagcg caatgagaaa agcgaaaagc cccacgcggc
     4201 cggcgagcat gttgacgata agaaggtatt tggaagggtc gcttaaatct ggagtaatgc
     4261 cccgcgacac gcctaccgta gcaaaagcgc tgcagttttc aaagaagata tccagagagc
     4321 tcatggaggg ctccagggcg caaatcagga cggtaaagca gccgatccag gcgcaggaga
     4381 ccaccacggt aatcacgcag cgcgccacgg tacgctggtc gatgcggcgg cggaaaaatt
     4441 ccacatgatc cctgccgcgg aggatgcgca ggatttccgc gcctgcaatg gcgcaggtag
     4501 tggttctcat gccgcctgcc gtgccgccgg ggctgcctcc caccatcatc agaccgcaca
     4561 taatgaggct ggctgcgggc agatagcggc tgtagtcgct gatattgaat cccgccgtgc
     4621 gtgccgtgac gctgtcgaaa aggcaaatcc agaggtgttc tcccaacggt tccgcggcat
     4681 ggggagagga aacggcaaag atggccaggg cgcctcccgc cagcaaaatc cccgtggaga
     4741 tcatcaccag tttgaaataa ggggtccact gcatgtgcag gcggcggtgt ttggaaaaga
     4801 agcggtttgc caggcgtgaa ttgatttcct tgtagatgcc gaatcccagg ccgccgcata
     4861 cgatcagcgc catgatgacc gcctgcccgc tgtagcacag gcggatgccc ggctccatca
     4921 aattcatcgg gaaagttgaa aaaccagcat tgcaaaaagc ggaaacggag tggaacaggg
     4981 aatgccacca caaaggctcc cccatcaggt tgatgtcctt cccttcccag gagaaataaa
     5041 gaaggacggc gccgcacagt tcgatgaaca gcgtactgac cacaatagtg gtgataaagg
     5101 agacgacggc ccccagattg tcttcatcaa gcagatccgt cagcagcacg cgatccctca
     5161 gggaaaaacc ctggcccgcc accatggcta cgaagtaggc gaacgtcatg atgccgaatc
     5221 cgccaatctg gataagggca agcgtgaaca tttctcccag cggggtgaaa gtatgggcaa
     5281 aatccacagc gttcagacca gtgacgcata ctgcgctggt gctggtgaac agggcatccg
     5341 taaaactgag tgtaataccc ggatatgtgg aattaggcag cagaagcaat ccggtgcctg
     5401 ccaggatgac tactcccaga gaagctacaa actgcatggc gggggccata agaggccggc
     5461 cttttttttg cttgcccacc agccattcaa aaatcctggt cagggacatc agcgtgggga
     5521 tggcaatcag cccggccgta atcaccaggc gggaaagctc cgtgaacctc atgtatcccg
     5581 cttcctgttg ttccctggca atcaggagaa aaatcatgag gcagctgatg atttggaaaa
     5641 tcaggagcct cagggaaggg cggttgattt cctttcggaa ataggaaccc agaatgcccg
     5701 tcaggtggcc cagcgtggca acggctgcaa tcagccgcga ccactcatgt tccgcccgcg
     5761 tcaggggcca gcccagctcc cagaccaccg cccccagaag aatggcaatg cagacggctg
     5821 caagtttggt gaatcccgag cgtgtagtgg tcatgaatgg aaatgcctta tgcggactct
     5881 accacctgga catggaaaga aaagctctta ggccgtttta tgcatgcggg cgggaaaggg
     5941 gcagatgtcg tactgcgttg cttcatgaca gggccgggcc agttgggaaa tggattaata
     6001 gtatttataa attttttaat tctggaaaaa ttttatataa atccaagttc gcgcaattgt
     6061 cgaatttgtt ccagctgctc gggtgttggg actggtttta cttcaggaac aggtgtgggt
     6121 tcgtattcgt caacccattt ggcgaaaagt cttgtcaact gatcggtaat ttccttgtcc
     6181 ctcaacttta tgtctgtcgg tatccaattg actcggttgg atttaccgaa atcttgagta
     6241 atcgcgatag ctgattttga atactgctcc ttctttcggt caaagtagtt attacttgca
     6301 cgaatattca ttggcttttc aagaggtatc ttgttaccaa tatgctcaag tgttttgttt
     6361 gtttcatctg aatcaagagt aaaaaacgat gtatcccact tctgggggaa aatgtgttca
     6421 atttcccatt tatctggaag caattcagac tggttttgat tatcgtaagc aagtatttta
     6481 agaagcattc tcaccattgt gcgtggtgga gttacgataa gggatattgc atcttcttcg
     6541 gaagtatctg ctttgaatcc tgccacaaat tctggttccg gagtcttgat aatggcggca
     6601 ttgagtttca atatggtgcc tttgactgcg gaaattgaag gcgattccaa atatcgcgtt
     6661 accaataata cacacaattt gcgtaagaat ttaagaaata atccctcgaa ttccgggtca
     6721 tctttgtgct gattgtagta tataattgta gggtatttcc aaaattcgtt attataagca
     6781 acaagtatat ccagggcttt gagaatatct ttattctcac tccatgactc atttgccaga
     6841 atttctccgc tgcggataac tttccataga ttgaggctat cggcgagtgt atcgatgatc
     6901 tcaggtttta gccggttctt actcttttct gtgaagtatt ttcttacacc ggacgtcgtg
     6961 gtcttgacat ctccgtcgca agcgcggaga taaaacatgt gatagttgaa taatgactga
     7021 atcgtttcac catgtttcga tgcttcagct tcaagttctt tccacttatg ggcaaaggat
     7081 ttcttttctc cagcagtgag cttcttgtat ataagtgact tgaaaatgtc ggcatccgaa
     7141 agtggcaatc ctctgttgtt aagtgtcgag aagatggtca aggctgtatc ctgattgtcg
     7201 gcagaaatag gtagaaggat tgaataattc agcaaagcat tgatgaagtg aaacatgtgc
     7261 atcgcgttat caactgcctt attctcataa agctcaacaa atttgttgaa gttatgagaa
     7321 taattatcct tggcatcctt ttcggtctga ccggtttcaa gaatgcggcg aagtatttcg
     7381 ttcccctggt ctgtggctac ctcagagcgg agtaaggttt tggttttgtc aacttcccca
     7441 gtcaattcat cttcaagcca taatgccggt tgaattttag agataaagtt ttttatttcc
     7501 tttgttttgt tttcctcgct ttcgagtttt gtatacacag cacgtagcaa caggaataac
     7561 gaggtaatgc gctgctgacc atcgataatt tctttctcgc cttgatcatt ttcaaaagaa
     7621 acgatgcttc cgagaaagta tgttttggca ctttttgacg tagtgcgttc gatagaaaac
     7681 tcc
//
LOCUS       DAOMSU010000088         7589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_749_length_7589_cov_2.502655, whole genome shotgun sequence.
ACCESSION   DAOMSU010000088 DAOMSU010000000
VERSION     DAOMSU010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_749_length_7589_cov_2.502655"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PK077_05205"
     CDS             complement(<1..419)
                     /locus_tag="PK077_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRN23731.1"
                     /translation="MKIPLFFNNTARSARAGRFRRWLDRHRDFFDVIEPESREDMLNH
                     LVSQANSGAPAVAVAGGDGTLGIAARALCNTETVLAPFPAGTMNVFSREIGIRPDFDH
                     ALHILNTGRSKNVDLFAFNGQVFLQMAGIGADARAVEL"
     gene            complement(416..1555)
                     /locus_tag="PK077_05210"
     CDS             complement(416..1555)
                     /locus_tag="PK077_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRN23732.1"
                     /translation="MNLLPFALLPGLTAAICAAHDAIQDGVEFLRAHMPAQDRGTVTQ
                     ERLVREVRLALAARSQFPWAAQVPWELYENNVLPYAVVNEPRDEWREQFHHLFAPLVS
                     PCKTGREAALAIASLIQKTLNVRYSTERRVPHQGVKESLQSGKVSCTGQSILLICALR
                     SVGIPARMAGVLTWNHVRGNHNWVEAWCDGEWKMLEYNEKDFNTPWVMSAISMLDPRK
                     PENAIYATSWKKEPSGAFFPMIWEARYDEKRHALAFPPESRTVPAVNITDRYMKLANE
                     WVVAQPEYVPGSRLMLDIREERKNGARRLPLHVVLKSEEGKVLAEGITPGPSDDMRKF
                     LEVLLPDNISRGMLEFKLPDGTVRHEPVAHTEAPVQILNFFVSAP"
     gene            complement(1568..2626)
                     /locus_tag="PK077_05215"
     CDS             complement(1568..2626)
                     /locus_tag="PK077_05215"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRN23733.1"
                     /translation="MHRPMLLALAPMKDVTDLAFLNTLKDLNSLPDYFITEYFRTVAH
                     HKKMSPYILRSIDENPTGRPIYGQLVGHEPEYLARDAQVLMEHACAGVDLNMGCPAPI
                     VCRRNAGGGMLRSLKAMDAALGALRDVLPPGAFTVKCRLGYETPDEFERILPVIASHS
                     PDRVCIHARTVREGYRSPVHPEWVKWAAGMLKCPVVANGNIVDAATAEAWVRLARPAG
                     LMIGRAALRNPWIFSQLHSRFQGHPAADLTFRNVLHYIRRLYERTREMQEHYVEEKHI
                     HRMKKYLVYTARGLPDTFDHYMKRAKTARDFMRICEDILDNDAPFAPTPPEDTHLFAH
                     FHTLLAQEEACLPPGIQV"
     gene            2697..3896
                     /locus_tag="PK077_05220"
     CDS             2697..3896
                     /locus_tag="PK077_05220"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HRN23734.1"
                     /translation="MNQDSTRKARVNVRRAEVMEQVEKEIQQHYQSELISHIRSAGNV
                     YNLGHTEFFLAREFGFCNGVRRAIDIAYAARRVFPDRRIFLIGDIIHNPEVNRQLEEM
                     GIRKLPWKQLDSSYDRVAPDDVVIIPAFGVPTPFMDALEEKGVQIVDTTCGDVMKVWK
                     RVKNYAAMGITSIIHGKATHEETSATASRALGERGRGKYLVVYDLEDARILCDYIMGR
                     GDREAFLKRFEGCCSPGFDPDRDLEEVGIANQTTMLKTETQTLQKMVKDAIVQRDGDD
                     DNFYVFDTICGATQDRQDALYELLKNPLDVMFVVGGYNSSNTTHLVDIAREHVPTYFI
                     ESAECIKSIQYVDAFDTKTREVRRMATEPVVQNLGKSLKVGITAGASCPANLIEATIL
                     RIADLRK"
     gene            complement(4030..4251)
                     /locus_tag="PK077_05225"
     CDS             complement(4030..4251)
                     /locus_tag="PK077_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23735.1"
                     /translation="MQQSRLDKAKLIIDRPGDFKVCEACGSIVSLTTAVCPNCNAYRF
                     DSNTEYVVEQAVLLASREQRSVTPEDLEI"
     gene            complement(4332..4595)
                     /gene="rpsT"
                     /locus_tag="PK077_05230"
     CDS             complement(4332..4595)
                     /gene="rpsT"
                     /locus_tag="PK077_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRN23736.1"
                     /translation="MANTKSALKRIRQTATRTARNRAVTSKLKTLRKKVAAAVETSDK
                     EAAAAAYNTFSSAVDKAAKVGIIPANRAANYKSKAAKAIAKIA"
     gene            4756..5364
                     /locus_tag="PK077_05235"
     CDS             4756..5364
                     /locus_tag="PK077_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HRN23737.1"
                     /translation="MKKRVDLPEKKYEGYIFDLDGTLVDSMPLHYRAWREALARAGAP
                     DHVFRADEFYSCGGKSANDVVRFLNERYGMHMDAASTAADKRRIYLEMLEKEGMQPIR
                     EVVEFVHSLGDAPKAIATGSAMPGASRTLAAAGLSGLFDVILTPDEVEHGKPAPDMFL
                     KAAELLGASPDRCVVFEDAEPGMKAAAAAGMDCVQVRRPSLS"
     gene            5514..7367
                     /gene="ilvB"
                     /locus_tag="PK077_05240"
     CDS             5514..7367
                     /gene="ilvB"
                     /locus_tag="PK077_05240"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HRN23738.1"
                     /translation="MSTNDKTSSAPTKTRMTGAEVLVECLVREGVDVVFAYPGGASMP
                     IHQALSHQPKIRTILPRHEQGGAFGAEGYGRVTGKVGVCISTSGPGATNMITSIADAY
                     LDSTPLVAITAQVMRSLIGRGAFQETDVFGMTAPIVKHSYLVTEPEELPRIIKEAFYI
                     ASTGRPGPVLIDMPKDVQEAVFTPDFDMEMDIPGYNPVLPVPADKLEELIPLIENARR
                     PVIYAGGGIISAEASGDLLEFAERSQIPVATTLMGIGAMPETHPLSLRWLGMHGAVFA
                     NNAVNEADLVIALGARFDDRVTGAVNMFCPESTIVHIDIDASEINKNKKVAYPIRANV
                     KDALRVLNSALAAKGWERKSSVYTRTPEWFGVIDGWKKQYPFSYEDRKGYIAPQAVIE
                     ELYRQTADKDPIICTGVGQHQMFAAQFFKFDKPRRLATSGGLGTMGYGLPASMGACLA
                     YPDRLVVNIDGDGSMLMNIQELATIHVERLPVKCIILNNQHLGMVVQWEDLKYDSNRA
                     QTFLADPHDNYDPTHKTEDVIYPNYPLICAGFGVKCERVLRIEELPAAITRMIESPEA
                     YVLDVMVPHDVHVLPMILGGMSYKDVILERIAGDGSAKKASELGKEIPTAL"
BASE COUNT         1662 a         1969 c         2237 g         1721 t
ORIGIN      
        1 agttctacgg ctcgggcatc cgcgccaatg cccgccattt ggagaaaaac ttgcccgttg
       61 aatgcgaaca ggtccacatt tttggaacgc cccgtgttca gaatgtgcag ggcgtggtcg
      121 aaatccgggc ggattcctat ttcccgtgaa aaaacattca tggtacccgc agggaagggg
      181 gccagcaccg tttccgtatt gcagagagcc cttgccgcga ttcccagcgt gccgtccccg
      241 cctgccacag caacggcagg cgctccggaa ttagcctgag aaaccaaatg gttcaacatg
      301 tcttccctgc tttccggttc aatgacgtca aaaaaatccc ggtgccgatc cagccagcgg
      361 cggaatcgtc cggctctggc gctgcgcgcc gtgttattga aaaacagggg aattttcatg
      421 gagcggacac gaagaagttc aaaatctgaa ccggggcttc cgtgtgtgcc acaggttcat
      481 ggcgcacggt tccgtcaggc agcttgaact ccagcattcc gcgggaaata ttgtccggca
      541 ggagtacctc aagaaacttc ctcatgtcgt cggacggtcc gggtgtaatg ccttccgcca
      601 gaactttccc ttcttccgat ttgaggacga cgtgcaaggg aagccttctg gcaccgtttt
      661 ttctctcttc cctgatgtcc agcatcagcc ggctgccggg cacatattcc ggttgggcca
      721 ccacccattc attcgccagt ttcatgtagc ggtccgtgat attgacggcg gggacggtac
      781 ggctttccgg agggaaagcc agcgcgtgcc gtttttcgtc gtagcgggct tcccatatca
      841 taggaaaaaa ggctcctgaa ggttcttttt tccaggaggt ggcataaatg gcgttctccg
      901 gtttgcgggg atccagcatg ctgatggcgg acatcaccca cggggtgttg aagtcctttt
      961 cattgtattc cagcattttc cattctccgt cacaccaggc ttccacccag ttgtggttgc
     1021 cgcgcacgtg gttccaggta agaacgcccg ccatgcgggc cggaatgccc acggagcgaa
     1081 gagcgcagat tagcaggatg ctttggcccg tacaggagac cttgccggat tgcagggatt
     1141 ccttgactcc ctggtgggga actcttctct ccgtggaata gcgtacattc agggttttct
     1201 ggatgaggga ggcgatggcg agggcggctt cccgccccgt cttgcatggg gaaacaagcg
     1261 gtgcgaagag gtgatggaac tgctccctcc attcgtctcg cggttcgttg accacggcat
     1321 aagggagaac attattttca taaagttccc acggaacctg ggcagcccag gggaattggc
     1381 tccgtgcagc cagggcgagg cggacttccc ggaccagcct ttcttgcgta accgttcccc
     1441 tgtcctgcgc gggcatgtgg gcacgcagaa actccacccc gtcctgaatg gcgtcatggg
     1501 cggcgcaaat agcggcggtc aggcccggga gaagggcgaa aggcagcaga ttcatgaacg
     1561 ttgaaattca tacctgaatt ccgggaggga gacaagcttc ctcttgcgca agaagggtat
     1621 ggaaatgggc aaagagatgc gtgtcttccg gcggagtggg ggcaaagggt gcgtcgttat
     1681 ccagaatatc ctcgcaaatg cgcatgaaat cgcgggcggt tttcgccctt ttcatgtaat
     1741 ggtcaaaagt gtcgggaagt ccccgagcgg tataaaccag atattttttc atgcggtgga
     1801 tgtgtttttc ctccacataa tgttcctgca tttcccgcgt gcgttcataa aggcggcgaa
     1861 tatagtgcag cacgttccgg aaagtaaggt ctgctgcagg atggccctga aagcgggaat
     1921 gaagctgtga aaatatccag ggattgcgca gggctgcccg tccaatcatc agcccagctg
     1981 gccgggccag ccgcacccat gcctccgctg tggctgcatc cacaatattt ccgttggcga
     2041 ctactgggca tttcagcatt cctgcggccc atttcaccca ttccgggtgt accggggagc
     2101 ggtagccctc acggacggtg cgggcgtgga tgcacaccct gtccggggaa tgggaggcaa
     2161 ttaccggaag aatccgttca aactcgtccg gcgtttcgta tcccaggcgg catttgacgg
     2221 tgaaggctcc ggggggcaat acgtcccgaa gcgctcccag ggccgcgtcc atggccttca
     2281 gggaccgcag catgcctccg cccgcatttc tccggcatac gatgggagcg gggcagccca
     2341 tgttcagatc cacgcctgca caggcgtgtt ccatcaggac ctgcgcatcc cttgccaggt
     2401 attccggttc atggcccacg agctgtccgt aaatggggcg gcctgtagga ttttcatcaa
     2461 tggagcgcag aatgtatggc gacatctttt tatgatgggc caccgtccgg aaatactccg
     2521 tgatgaaata atccggcagg gaattcaagt ccttcaatgt gttgaggaaa gccagatccg
     2581 tcacatcttt catgggggcc agggccagga gcatgggtct gtgcataccg tgaaaagctc
     2641 cggttggcaa gctgtgattg tcttgacgaa agaaggaaag tgtggaagct gggagcatga
     2701 atcaggactc aacccgcaaa gccagagtga atgtgagacg ggccgaagta atggagcagg
     2761 tggaaaagga aatccagcag cattaccaga gtgaattgat ttcccacatc cgttctgcgg
     2821 gcaatgtgta caatctgggc catacggaat tttttctcgc ccgggagttc gggttctgca
     2881 acggcgtgcg ccgtgctatt gatatagcat atgcggcacg cagagtgttt cctgaccggc
     2941 ggatttttct gattggggac attattcaca atccggaagt gaaccggcag ctggaggaaa
     3001 tgggcattcg aaaacttccc tggaagcagt tggattcctc ctatgaccgg gttgctccgg
     3061 acgatgtggt gattattccc gcgttcggtg ttcccactcc tttcatggat gcgctggagg
     3121 agaagggcgt gcagattgtg gatacgacat gcggagacgt gatgaaggtc tggaaaagag
     3181 tgaagaatta cgccgccatg gggattacct ccattatcca cggtaaggct acccatgagg
     3241 aaaccagcgc tacggcttcc cgggctcttg gggaacgggg aagggggaaa tacctggtgg
     3301 tttacgattt ggaggatgcc cgtatcctgt gcgactacat catgggccgc ggagaccgcg
     3361 aggcattcct gaaacggttt gaaggatgct gttccccggg attcgatccc gaccgggatc
     3421 tggaggaggt tggcatcgcc aaccagacca ccatgttgaa aacggagacg cagacgctcc
     3481 agaagatggt gaaggatgcc attgttcaga gggacgggga cgatgataat ttttatgtgt
     3541 ttgacaccat ttgcggtgct acccaggatc gccaagatgc cctgtatgaa ctgctgaaaa
     3601 atcctctgga cgtcatgttt gtggtgggtg gctacaacag ttccaacaca acgcatctgg
     3661 tggatattgc cagggagcat gtgcccacgt acttcattga gtccgcagaa tgcatcaagt
     3721 ccatccagta tgtggatgct tttgatacga agacgcggga agtgcgccgc atggctacgg
     3781 aaccggtagt gcagaacctg ggcaaatccc tgaaggtggg aattacggcg ggcgcctcat
     3841 gcccggccaa cctgattgag gccaccatcc tccgcattgc ggatctgcgc aagtagcaaa
     3901 acgcctccgg tttggtgaat gagaaaaaag agttctcttt caacggcggc acggccgttt
     3961 tttccgtaac acggcgcagt tgcctgggcc gcggaagaaa gcatagggaa aactgcgcgg
     4021 gagcgtttcc taaatttcca aatcttccgg tgtaacggaa cgttgttccc gggatgcaag
     4081 cagtacagct tgttccacga catattccgt attggagtca aaacggtagg cgttgcagtt
     4141 gggacacact gccgtagtca gggatacgat ggaaccgcag gcttcgcaaa ccttgaagtc
     4201 cccgggacgg tcaataatca gtttggcttt atccaggcgg gattgctgca tgggagtaga
     4261 aaataaaaca agccgctcag cagaacagag cggcttgcca taaagaatcg tcagaaaacc
     4321 gaagtgaaaa ctcaggcgat ttttgcgatg gccttggcgg ccttgctctt gtagttggct
     4381 gcacgattgg cggggatgat gccaaccttg gcggctttgt caacggctga ggagaaagtg
     4441 ttgtaggcag cagcagcggc ttccttgtcg gacgtttcca ccgcagcggc aactttcttg
     4501 cggagggtct tcaacttgga ggtcacggcg cggttgcggg cggtgcgggt tgctgtctgg
     4561 cggatgcgct tgagtgcgga tttggtattg gccatgttta aaaaaggttc ggtttccgtt
     4621 tcgaggtggc aaaaacatta gaataacagg acaaaatgtc aagctcttga tgtatttttt
     4681 aaggcattcc tttcctgcac gggctgactg tgggctttac cggaaaagga gtttgtggca
     4741 ctatattctc ccgttatgaa gaaacgcgtg gatttgcctg aaaaaaaata tgaaggatat
     4801 atttttgatt tggacggcac cctggtggac agtatgccgc tccattacag ggcatggcgt
     4861 gaagccctgg ctcgagcggg ggctccggac catgtgttcc gggcggatga attttattcc
     4921 tgcggcggaa aatccgccaa tgatgtggtg cgttttttaa atgaacggta cggcatgcac
     4981 atggatgcgg cgtccacagc tgcggacaag cgccgcattt atttggaaat gctggaaaag
     5041 gaaggcatgc agcctatccg ggaagtggtg gaattcgtgc attccctggg agacgccccc
     5101 aaggcgatag ctaccgggag cgccatgccg ggagcgtccc ggactctggc tgctgccgga
     5161 ctttcaggcc tttttgatgt gattttgacg ccggatgagg tggagcatgg caaacctgct
     5221 ccggacatgt ttttgaaggc ggcggaactc ctgggggcat ccccggaccg ttgcgtggtt
     5281 tttgaggatg ccgaacccgg catgaaagct gccgcggccg caggaatgga ctgcgtacag
     5341 gtccggcggc catccctgtc ctgattcctc tttccggatt tggtacggaa ggggattccg
     5401 ggattttcct cctttctcca tgcccttctt ccttttaagg aaaaggcttc acattgcgcc
     5461 cggaatgcgt tagaaccttt tttcctattg aacaaataaa atattatttg cacatgagca
     5521 caaacgacaa aacatcatcc gccccgacga aaacccgcat gacaggagct gaagtgctcg
     5581 tggaatgcct cgtgcgtgaa ggtgtagacg tcgtctttgc ctatcctggc ggcgccagca
     5641 tgccgattca tcaggccctg agccatcagc ccaagatacg gaccatcctg ccccgccatg
     5701 aacagggcgg ggcattcggc gcggaaggct atggccgcgt tacgggcaag gtgggcgttt
     5761 gcatttccac ttccggcccc ggcgccacca acatgattac ctctattgcg gatgcttatc
     5821 tggactccac gcctctggtg gcgattacgg ctcaggtcat gcgctccttg attggacgcg
     5881 gcgccttcca ggaaacagac gtattcggca tgaccgcccc tatcgtgaag cacagctatc
     5941 tggtgacgga accggaagaa ctgccccgta tcatcaagga agccttctac atcgcctcta
     6001 cgggccgtcc gggccctgtg ctgattgaca tgcccaagga cgtgcaggag gccgtgttta
     6061 caccggactt tgacatggag atggatattc ccggctacaa ccccgtgctg cccgtccctg
     6121 cggataagct ggaagaactc atcccgttga ttgaaaacgc caggcggcct gtcatttacg
     6181 ccggcggcgg catcatttct gcggaagctt ccggggatct tctggaattt gcggaacgtt
     6241 cccagattcc tgtggcgacc accctgatgg gcattggcgc catgccggaa acccaccctc
     6301 tttccctgcg ttggctgggc atgcacggag ccgtgtttgc gaataacgcc gtgaatgaag
     6361 cggatctggt tattgccctg ggcgcccggt ttgacgaccg cgtgacaggc gccgtcaata
     6421 tgttctgtcc ggaatccacg atcgttcaca ttgatatcga cgccagcgag atcaataaaa
     6481 acaagaaggt ggcgtatccc atccgcgcaa acgtgaagga cgccctccgg gtgctcaact
     6541 ccgctctggc cgcaaaaggc tgggagcgca aatcctccgt ctacacccgc actcctgaat
     6601 ggttcggcgt gattgacggc tggaaaaaac agtacccctt ctcctatgag gaccgcaaag
     6661 ggtatatcgc cccgcaggct gtcattgagg agctctaccg ccagacggcg gacaaggacc
     6721 ccatcatctg cacgggggtg ggccagcatc agatgtttgc cgctcaattc ttcaaatttg
     6781 acaagccccg ccgtctggcc acttccggcg gtttgggaac catgggctat ggacttcccg
     6841 cctccatggg agcgtgcctg gcttatccgg acagactggt tgtgaatatt gacggggacg
     6901 gttccatgct catgaatatt caggagctgg caaccattca tgtggagcgt ctgcccgtca
     6961 aatgcattat tctgaacaac cagcacctgg gcatggtggt ccaatgggaa gatttgaagt
     7021 atgattccaa ccgggcccaa accttcctgg cggacccgca cgacaattat gatcctaccc
     7081 ataaaacgga agatgtcatc tacccgaact atccacttat ttgtgccggg ttcggcgtca
     7141 agtgcgagcg tgtgcttcgc atagaggagc ttccggcggc tatcactcgg atgattgaat
     7201 cccctgaggc ctatgtcctg gacgtcatgg ttccccatga tgtgcatgtg ctgccgatga
     7261 ttctgggggg catgagctac aaggatgtga ttctggaacg tattgccggg gatggttccg
     7321 ccaaaaaggc gtctgaactg ggcaaggaaa tcccgactgc cctgtaagag cgaggcaagc
     7381 ttcttctatt gagccttaaa caggagccgt ccgtaagggc ggctcctgtt gcatggcggc
     7441 gcaagtggaa tgcaggtcag ttgactgcca ttagggtggc agcaaggcat gtatatagat
     7501 ccggatattc ctgttttccg gtaggtgaat tcctggtttt gtgaaggaat gaggaagatg
     7561 tttctggacg cagtaaaaaa gaacggtta
//
LOCUS       DAOMSU010000089         7581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_750_length_7581_cov_2.222828, whole genome shotgun sequence.
ACCESSION   DAOMSU010000089 DAOMSU010000000
VERSION     DAOMSU010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7581
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_750_length_7581_cov_2.222828"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>680
                     /locus_tag="PK077_05245"
     CDS             <1..>680
                     /locus_tag="PK077_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23739.1"
                     /translation="THMGQLEWRDIRGGVSPDKLDRLLLIARLASAAPLFKENENTPF
                     EQMTQDFTSARDISLKGKVMKNLLIISKINLRQQCSAVRKKEVGQLCSIYAATMLKMM
                     KEAYIPDFEGWKKEIMESKTAVWNKEFNARWESFLKERDERSERARKELSIPFKQRMR
                     MRQQKTEEERMRDREFGEKDAAFMQEAREKWNKRMNLDVSLSMMDGRNSKNKSFLKYK
                     GGSLLRIL"
     assembly_gap    681..758
                     /estimated_length=78
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            961..2241
                     /locus_tag="PK077_05250"
     CDS             961..2241
                     /locus_tag="PK077_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23740.1"
                     /translation="MNTFVSLCLVGSLSLGSLCYAQYMDNDAKKKVIKDLDNLLALNR
                     SAETKVINAAISKLNSAGRDPGAAYDLLIQSKKFLMETEKEKDKSQGKSAKIASNSMK
                     DWMDREAKKYQDKNARKGLMLQYQWGVLVLKARLKENLMNATSHSTGGTAEETLDVTE
                     FQKPAMDLLNAYLSACSDPDFIDPSSSKNNNGAAGRAGARIAEQSVLNTEVGEALGIS
                     SMSSKAFPDSMKNRDEIFDKLFFTGYRKTRNLPALRQAWNKRISMEVALGKISHAISS
                     GKKSGMQGRIANASEDEFDEQGAAVLDRLRWRCELDCFDLGDQANATRNMMTLIRTTV
                     DPVRREQGIRTLRDKLVQSMKSMMDSPEEGSMADSSALSPAPRPAVRAPKKKTPSTTI
                     TTSREMDDPGAIGTDAPSPPKPDPTRSDDFFEDV"
     gene            2367..3503
                     /locus_tag="PK077_05255"
     CDS             2367..3503
                     /locus_tag="PK077_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRN23741.1"
                     /translation="MPAQIFFGAGSLDNLGSAPLPGAKALIVIGGTSVKRLGYLDRVQ
                     ALLKKQGVESVVFDKVQPNPVVEHVMEASSLARETGCDFVIGLGGGSSMDSAKSIAVM
                     AANPGTYWDYIQGGSGKGLSIPCKPLPIVCITTTAGTGTEADPWTVITKEDTQEKIGF
                     GFRGTFPTMSIVDPELMLSVPPKLTAYQGFDALFHAVEGYMATIASPMGDMFALQAIE
                     YIAKYLPRAVNNGDDLEARAYVALANTYSGFVETISCCTSEHSIEHALSAFHPSLPHG
                     AGLIMISWAYHEAYAPSCPERYARVAAAMGQEASVDGFLDGLNSLKEACGVDKLKMSE
                     FGITPDLFDEYAKTAFSTMGNLFELDRCKLTPADVVSILEKSYS"
     gene            complement(3730..4098)
                     /gene="crcB"
                     /locus_tag="PK077_05260"
     CDS             complement(3730..4098)
                     /gene="crcB"
                     /locus_tag="PK077_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HRN23742.1"
                     /translation="MIKMLMMVGLGSFAGGILRFLLSRFVQVNSASSFPWGTAAVNLL
                     GCFILGALYGLFDRNLPHTETRLFLTAGLCGGFTTFSTLMNESFLLLKEGNFSAFFLY
                     TLISFAGGLLAIGLGYLAAR"
     gene            4378..5340
                     /locus_tag="PK077_05265"
     CDS             4378..5340
                     /locus_tag="PK077_05265"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRN23743.1"
                     /translation="MSQPLLVTGTIGIDTILTPGGRADGVLGGSVSFAAVAALLFADR
                     VDAVSIIGEDFPAHYEAALEAKGVCMNGVERKKGPSFSWTGEYFDNMNKRRTVCANDS
                     VMLEWDVHVPEPLRNHPVLVCCCMVPAQQLKCMEQCPDAALVLSDSMDKWLRRQPELL
                     DAVISRSHITMMNEDEAKEYAQASTVMEAGEFLLSKGAAYAVVKQGEYGATLLGRDAG
                     GNLRMFRCPAYPLKTLVDPTGAGDTFLGALAGYLSTLPPGLPPFEEVKKGIVYGTVAA
                     SFTCESFSADALLSMTKETFRRRLEEYAEVCRLPDIRLFSASDC"
     gene            complement(5521..6837)
                     /locus_tag="PK077_05270"
     CDS             complement(5521..6837)
                     /locus_tag="PK077_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HRN23744.1"
                     /translation="MACGNQRTVGSPASLAGTSLHTGQPVTLTLKPAPADFGIKFRRV
                     DIPDQPFISADVEKVQTVERATSLAEGSVKVHTVEHILSALTGMGIDNAVIEMDANEP
                     PIGDGSSAPYVELIKSAGIVELDVPRRYLEVREAVTIETKGGSILTILPSKQFRVSVT
                     CVGPENRITQYFDAVITPETYEKELAPARTFTFYEDIKPLLEKGLIKGGSLENAVVIR
                     GEELMSKEPMRFTNEFARHKAMDLIGDLTLCGKPILGHVIAIKPGHGPNTELTAKLKK
                     EHHRNQQMAPNPINVPYGDAVLDINEVMSLLPHRYPFLMVDRIIGFEGETKCRGLKNL
                     TMNELFFQGHFPGHPVMPGVLQVEAMAQVASIAMLRQPGNASKLGYFMSADKVKFRRV
                     VVPGDTLIIEAELTKMRGNIGQATARCLVNGQVVSEAELKFGLQDA"
     gene            6993..>7581
                     /locus_tag="PK077_05275"
     CDS             6993..>7581
                     /locus_tag="PK077_05275"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRN23745.1"
                     /translation="MHITAGIRISRAFILGAGLGTRLRPLTGVLPKPLIPFFHEPLIL
                     HSMRRCYDCGIREFIINTHHLAAAWDKVFPERSWNGCPVHFSHEPVLLDSGGGVKKIE
                     PLASAEEPLLVVNGDMAATFDLGRLLEEHLSRRPPVTLALRTSGDKKNVGFDFSSGLV
                     TDMRHALGRDSGSCQFTGAYCMEPEVFGRIPSGEAV"
BASE COUNT         1718 a         2017 c         2126 g         1642 t
ORIGIN      
        1 cacccatatg gggcagcttg agtggagaga catcagaggg ggagtttccc cggacaagct
       61 ggatcggtta ttgttgattg cccggctagc ttccgcagcc cccctgttta aagagaacga
      121 gaacacgccg ttcgaacaaa tgacgcagga tttcacttca gcaagagata tcagtctcaa
      181 ggggaaagtt atgaaaaacc tgctcatcat ttccaaaatc aatcttaggc aacaatgctc
      241 tgccgtgaga aagaaagaag tggggcagct ttgctccatt tacgcggcaa cgatgctgaa
      301 gatgatgaaa gaagcctaca ttcctgattt cgagggctgg aagaaggaaa ttatggagag
      361 caaaacggcg gtttggaaca aagagtttaa cgccaggtgg gagtccttcc tgaaagaaag
      421 agatgaaaga agtgagcgag ccaggaagga gctatccata cccttcaaac agagaatgag
      481 aatgagacag caaaaaacgg aagaggaacg aatgagagac agggaatttg gggagaaaga
      541 cgccgccttc atgcaggaag caagggaaaa atggaataaa cgaatgaatc ttgatgtcag
      601 cctttctatg atggatggtc gaaactccaa aaacaaatca ttcctcaaat ataagggagg
      661 atccttattg cgcattctgt nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnncc gggaaatggg aaaaaacaag
      781 gatacggagt tcctgtatca aagttccttg ggaagaaagt tccttaaaga gaagaaacaa
      841 aagaaataat tcctgccaga gcgcggaagc atgccatttt tagcctgcaa gggtatttta
      901 tctttttgag attccttctc tcttgccaga tcacctttca tccttcataa taaaaaaagc
      961 atgaatactt tcgtttctct ttgcctggtc gggagcctca gcctgggttc cctttgttat
     1021 gcgcagtaca tggataacga tgccaagaag aaggtaatca aggatttgga taatttgctc
     1081 gctctgaaca gatctgcaga aaccaaggta atcaatgccg ctatcagcaa actgaattcc
     1141 gccggcaggg atcccggcgc cgcatatgac ctgctgatcc agtccaagaa atttctgatg
     1201 gaaacagaaa aggagaagga caagagccag gggaagagcg ccaaaatcgc ctccaattcc
     1261 atgaaggact ggatggatcg tgaagcgaag aagtaccagg ataaaaatgc ccgtaaagga
     1321 ttaatgcttc aatatcaatg gggagttctc gtcctcaagg cccgtcttaa ggaaaattta
     1381 atgaacgcca cctcccattc caccggaggg acagcggagg aaaccctgga cgttacggag
     1441 ttccagaagc cggccatgga tttgctgaat gcctacctgt ccgcctgctc cgaccctgat
     1501 tttatcgacc cttcctcttc caaaaataat aatggagcgg ccggaagagc cggcgcccgt
     1561 attgcggaac aatccgtcct taatacggaa gtgggggaag ccctgggcat cagcagcatg
     1621 tcttccaagg ctttcccgga ttccatgaaa aaccgggatg aaatttttga caagctgttt
     1681 ttcaccggat accggaagac ccgcaacctt cccgccctca ggcaggcatg gaacaagaga
     1741 atttccatgg aagtggctct gggaaaaatc agccatgcca tttcctccgg caagaagtcc
     1801 ggcatgcagg gccgcatcgc caatgcgtcc gaagacgaat ttgatgagca gggagccgcc
     1861 gttctggatc ggctgcgctg gcgttgcgaa ctggattgct tcgacttggg agaccaggca
     1921 aacgccacga ggaatatgat gacgctcatc cgaacgacgg tggatcccgt gcgccgggaa
     1981 cagggtatcc gcaccctccg ggataaactg gttcagagca tgaagagcat gatggattct
     2041 ccggaagaag gaagcatggc tgacagttcc gctttatccc ctgctccgcg ccctgccgtg
     2101 cgcgcgccca aaaagaagac tccctccacc acgatcacga ccagccgtga aatggatgat
     2161 cccggtgcca ttggaacgga tgcgccgtca ccgcccaaac cggatccaac ccggagcgac
     2221 gatttttttg aagacgttta acgttcttca gacaaagatg ctgcccgaac ggcggtttcc
     2281 ctgttcatgg aatgcacaga gcatccttga cgtccggaac cgccgtttta tacagtaagg
     2341 gcatgtatca gccatttcaa tttttcatgc ccgcgcaaat cttcttcggc gcgggttctt
     2401 tggacaatct tggttccgcc cccctgcccg gcgccaaggc cctgatcgtc atcggcggga
     2461 cgtccgtcaa acgcctcggg tatctggacc gcgtacaggc tcttctgaaa aaacagggag
     2521 tggaaagcgt tgttttcgat aaagtgcagc ccaaccccgt ggtggagcac gtaatggaag
     2581 cctcctccct ggccagggaa acaggctgtg atttcgtcat cggcctgggc gggggcagca
     2641 gcatggattc cgccaagagc atcgccgtga tggcggccaa tcccggaacc tactgggatt
     2701 acattcaggg cggttccggc aaggggcttt ccattccctg caaacctctt cccatcgtct
     2761 gcatcaccac tacggcggga accggaacgg aggcggatcc gtggaccgtc atcacgaaag
     2821 aggacacgca ggagaagatc ggtttcggct tcaggggtac tttccccacc atgtctatcg
     2881 tagatccgga gttgatgctt tccgtacctc ccaaattaac ggcataccag gggtttgacg
     2941 ccctgttcca tgccgtggag ggatatatgg ccacaatcgc ctcccccatg ggggacatgt
     3001 tcgcgctcca ggccattgaa tacattgcca aatatcttcc gcgcgccgtg aataacgggg
     3061 atgacctgga ggcgcgcgcc tatgtggcgc tggccaatac ctattccggg tttgtggaaa
     3121 ccatttcctg ctgtacgtcg gaacattcca ttgaacatgc cctcagcgcc ttccatcctt
     3181 ccctgcccca tggcgcgggg ctaatcatga tttcctgggc ctaccatgaa gcctatgctc
     3241 cctcctgccc ggaacgttac gcaagagttg ccgcagccat gggacaggaa gcctccgtgg
     3301 acggtttcct ggacggcttg aacagcctga aggaagcctg cggcgtggac aagctgaaga
     3361 tgtccgaatt cggcattaca ccggatttat ttgacgaata cgccaaaacg gccttttcca
     3421 ctatgggcaa tctgtttgag ctggaccgtt gcaagttgac tccggcagac gtggtcagca
     3481 tcctggagaa atcctattcc taagaataag ccgccgggga tcccatgccg ggctggctac
     3541 cgcccgcccg gcagaatttt ttgctgttcc aatgcattgg gaatgccgtt aatttccatc
     3601 atgtttgggc aactcccgga attaaagggg gggggagcgt ggttctgctg gccgtccatt
     3661 tccatttcaa gagttccgct gtaaaacagc gcggcaggcc atgcgcagcg ccgggtttcc
     3721 atgtttttct taccgggcgg ccagatagcc caggccaatg gcaaggagcc ccccggcaaa
     3781 gctgatgaga gtgtaaagga agaaggcgga gaagttccct tccttcaaca acaggaagga
     3841 ttcattcatc agcgtggaaa aggtggtgaa accgccgcac aggcctgcgg taagaaagag
     3901 ccgcgtttcc gtatgcggca ggttccggtc gaacaaaccg tagagggcgc ccaggataaa
     3961 acatcccagc aggttgacag cagccgtacc ccaggggaac gaacttgccg aattgacctg
     4021 gacgaaacgg gacagcagga aacgcaatat gccgcccgca aagcttccca gacccaccat
     4081 catcagcatt tttatcatag cggcttttct acatttgaat agaaaataaa gttccggagc
     4141 aatcccgaaa tcttccggga aaaagaactg ccggctacgt tcctgaaaac tggtctggaa
     4201 acatccgtgc ctcctggtga agccctttaa ctggaaacag acttctgcgg aagctgtcgg
     4261 ccagtatgcc ggcagaaccc atttccttta acgcgtgcgg ataaaatacc ctgttttttc
     4321 catatcgcca gcctttttgt tcttgattgc agggtaaaac cgtatttccc tttttccatg
     4381 tcccagcccc ttctcgttac cggaaccatc ggaattgata ccatccttac tcccggcggc
     4441 agggcggacg gcgttctggg cggttccgtc tcctttgcgg ccgtggccgc ccttttgttt
     4501 gccgaccgcg tggatgccgt cagcatcatc ggggaggatt tccccgccca ttatgaagcg
     4561 gctcttgagg ccaaaggggt gtgcatgaac ggtgtagaac gcaagaaagg gccttctttt
     4621 tcctggaccg gggaatattt tgacaatatg aacaagcgga ggaccgtttg cgccaatgat
     4681 tccgtgatgc tggaatggga tgtgcacgtt ccggagcctt tgaggaacca tccagttctg
     4741 gtttgctgct gcatggttcc ggcccagcag ctcaagtgca tggaacaatg tccggacgcg
     4801 gcgctggtgc tttccgattc catggacaag tggctgcgcc gccagccgga attgctggat
     4861 gccgtgatca gccgttccca tatcaccatg atgaatgagg atgaggcgaa ggaatatgcc
     4921 caggcgtcca ccgtcatgga agccggggaa ttcctgcttt ccaaaggcgc cgcgtacgca
     4981 gtggtgaaac agggggaata cggagccacg cttctgggcc gggatgccgg cgggaatctc
     5041 cggatgttcc gttgtcccgc ctatcctctg aaaacgctgg tggaccccac cggcgcgggg
     5101 gatacgttcc tgggggctct ggccggctat ttgtccaccc tgcctcccgg ccttcctccg
     5161 tttgaggaag tgaagaaggg aattgtgtat ggaacggtgg cggcctcctt cacctgcgag
     5221 tccttttcgg cagatgctct tctttccatg accaaagaga cctttcgccg ccgtcttgaa
     5281 gagtacgcgg aggtgtgccg cctgcctgat atccggctct tttccgcaag tgactgttaa
     5341 gacgggatca gatctcctga aaaggaggaa ataccgccct tgatttcgtt ttccggtctc
     5401 cgtttttgcg ggatgtccgg aatgttcagg taaagaaagc ccggatgcgg aacatgaaaa
     5461 accccggcgt ggcatgcacg gccggggttt ccgtaaaaac aggggatgca gtcggcggga
     5521 tcaagcatcc tgaaggccga atttaagctc ggcttccgaa accacctgtc cgttcaccag
     5581 gcagcgtgcc gtcgcctggc caatgtttcc gcgcatcttg gtcagctccg cttcaatgat
     5641 gagcgtatcg ccgggaacca cgacgcggcg gaattttacc ttgtccgcac tcatgaagta
     5701 accgagcttg ctggcattgc cgggctggcg cagcatggcg atggaggcca cctgggccat
     5761 ggcttccacc tggagaacgc ccggcatgac cggatggccc gggaagtggc cctggaaaaa
     5821 gagttcgttc atggtcaggt tcttcagacc ccggcacttg gtttctcctt caaaaccgat
     5881 aatgcggtcc accatcagga agggatagcg gtgcggcaga aggctcatta cttcgttgat
     5941 gtccagcacg gcgtctccgt acggcacgtt gatgggattg ggagccattt gctggttgcg
     6001 gtggtgttcc ttcttgagct tggccgtgag ttcggtgttc gggccgtggc ccggcttgat
     6061 ggcgatgacg tggcccagaa tgggcttgcc gcacagggtc agatccccaa taaggtccat
     6121 ggccttgtgg cgtgcgaact cattggtgaa acgcatgggt tccttgctca tgagctcctc
     6181 gccgcggatg accacggcat tctccaggct gccgcccttg atgaggccct tttccagcag
     6241 gggcttgatg tcttcgtaga acgtaaaggt gcgggccggg gccagttcct tttcatacgt
     6301 ttccggcgtg atgaccgcgt caaagtactg ggtgatgcgg ttttccggtc ccacgcaggt
     6361 gacggaaacg cggaattgct tggaggggag aatggtcagg atggaaccgc ccttggtttc
     6421 aatggtgacg gcctcgcgca cttccagata gcggcgcggc acatccagtt ccacgatgcc
     6481 ggcgctcttg atcagttcta cgtagggggc tgaggacccg tccccgatgg gcggttcatt
     6541 ggcgtccatt tcaatgacgg cgttgtcaat gcccatgccc gtgagggcgg agaggatgtg
     6601 ttccacggta tgcaccttga cggaaccttc agccaggctg gtggcgcgtt ccacagtctg
     6661 caccttttcc acatccgcgc tgatgaaagg ctggtccgga atgtccaccc ggcggaattt
     6721 aatgccgaag tcggcgggag ccggcttcag ggtcagggta acgggctgcc ccgtatgcaa
     6781 agaggtaccg gccagggagg ccggagagcc gacagttctt tggtttccac atgccatgcg
     6841 gggatgctag tgaatgagct tccgtttgga aagcacgaaa tcacggattt tcctttaaat
     6901 tcagggaaga ggcggtattc cggcgtgtca tcccggaaag aagtggataa agggcgcttg
     6961 aaaaaacgcc gctcacccgg tagtgatgtt ccatgcacat cacggcaggc atccgcattt
     7021 cccgtgcgtt tattctcggc gccgggctgg gtacccgcct gcgccctctc accggcgttc
     7081 tgcccaagcc gctgattccc tttttccatg agccccttat tctgcattcc atgcgccgat
     7141 gctacgattg cggcatacgg gagttcatca tcaacaccca ccatctggcg gcagcctggg
     7201 acaaagtgtt ccctgaacgt tcatggaacg gctgccccgt ccatttcagc catgaacccg
     7261 tgctgctgga ttccggaggc ggggtgaaga agatcgaacc cctggcctcc gcggaagaac
     7321 cgctgctggt ggtgaacgga gacatggcgg ccacatttga tctgggccgc ctgctggagg
     7381 agcatttgag ccgccgccct cccgtgacgc tggccctccg cacctccgga gataagaaaa
     7441 acgtgggatt tgatttttct tccggcctgg tgacggatat gaggcacgcc ctgggccgcg
     7501 actccggctc ctgccaattt accggcgcgt actgcatgga gccggaagta ttcgggcgca
     7561 tcccttccgg ggaggccgtt t
//
LOCUS       DAOMSU010000090         7511 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_758_length_7511_cov_2.643374, whole genome shotgun sequence.
ACCESSION   DAOMSU010000090 DAOMSU010000000
VERSION     DAOMSU010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7511)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7511
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_758_length_7511_cov_2.643374"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="PK077_05280"
     CDS             <1..663
                     /locus_tag="PK077_05280"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HRN23746.1"
                     /translation="LKGVGEFRHFNDFFYRRLKPGARPLAGGEDTAVFPADARHMGWE
                     RADRIKNVFVKGQRFDLPSLLGSDTLAERYAAGAVVLSRLCPTDYHRFHFPVSGVPGP
                     WTKLGGPLASVSPYCLRGRLAWLWTNKRNLTLIRSELWGEVAMLEVGATGVGLIEETY
                     VPGVFSARGAEKGYFAFGGSTVMCFFEPGRVSLASDLLEKTEEGLELFARQGDMMGTA
                     AT"
     gene            complement(792..1445)
                     /gene="rpe"
                     /locus_tag="PK077_05285"
     CDS             complement(792..1445)
                     /gene="rpe"
                     /locus_tag="PK077_05285"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HRN23747.1"
                     /translation="MTIISPSLLAADFSRIGEEARRAFDSGADWLHLDIMDGHFVDNI
                     SFGPEICAAVRKAVGPDRFLDAHLMIERPDHYFDRFVRAGVNLICFHVELGDSYYIRN
                     TLGRIREAGVKNGLALNPATSFEAVEPYLAEIDLLLVMSVVPGFGGQSFMPSVLDKVE
                     KAAEWRTENRASFLIQMDGGIGQNNAAQCALAGADVLVAGSSTFKAPDMARAIAEMK"
     gene            complement(1470..4043)
                     /gene="leuS"
                     /locus_tag="PK077_05290"
     CDS             complement(1470..4043)
                     /gene="leuS"
                     /locus_tag="PK077_05290"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRN23748.1"
                     /translation="MSERKKPYPFDVFEPKWQQIWDERKTFKVNNPGEEGFDASKPKY
                     YVLDMFPYPSGAGLHVGHPEGYTATDIVARFKRMNGFNVLHPMGWDSFGLPAEQYAIK
                     TGQHPSVTTFRNIDNFRRQLKMLGFSYDWDREIATTDHEYVRWTQWIFLQLYNSYYNK
                     ELKKARPVSELEEQGLSREEIDQRRLAYVAEAAVNWSPDLGTVLANEEVEEWKSKGHR
                     VERRPLRQWMLRITDYAERLIDELEPLDWPESIKLLQRNWIGKSEGAEVDFTLDGETI
                     TVYTTRPDTLFGATYMVLSPEHPLVDTVTTPEQKHAVEQYRAQCASKSDLERTDLSKE
                     KTGVFTGAYAVNPVNGKQIPVWIADYVLMGYGTGAIMAVPAHDERDFAFAQVFGLPIL
                     QVVQPPSEDMDWRGFCGYEGSSVNSGFLTGLPTPEAKEKMILWLEENGKGRRKVNYKL
                     RDWLFSRQRYWGEPFPIVWENGHHRALPESELPVLQPDLDDFAPTGDPRGPLVKAAEW
                     IAYTPTAHRETNTMPQWAGSCWYYLRYLDPANTERFVSREAEQYWMGSAGSPGGVDLY
                     VGGTEHAVLHLLYARFWHKVLFDLGYLSTNEPFQKLVNQGLILGEDGQKMSKSRGNVV
                     NPDDIVREYGADSLRLYEMFMGPLKDVKPWATKGVEGISRFLARVWRVAFRENQEGEW
                     EINSKLVENAPEAGVLAVRKELHKTIKKVTEDINGMSFNTAIAKMMECTNAMTSADVV
                     DVQDYDAFLTLLNPFAPHLTEEIHSRLQTAFPALAQTQLCQKSWPEWEEALLEENTVP
                     MVIQVNGKLRDKLEVPKDISREELEKQALASAKVKTFLDGVTVRKVIVVPGRLVNIVA
                     N"
     gene            4213..5898
                     /locus_tag="PK077_05295"
     CDS             4213..5898
                     /locus_tag="PK077_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRN23749.1"
                     /translation="MKFTSCIMSAVCGALFLFAGASALGNGSNPVSGKKPNIIVFLVD
                     DMGWQDTSHPFWSDSQGNPKKTFLNRRYRTPNMEKLASQGMTFTDAYAHPLCTPSRVS
                     LMSGMNPARHRVTCWVREQNGTTDANSRSLLPPDWALNGLQPIGTPARGTTKRPISGE
                     DMRYHMTRPFATAATLPEMLKKCGYVTVHCGKAHFGTQGTPGSNPLNMGFDYNIAGTE
                     IGHPADYRGSRHYGKGFNHVRGLDENNYYQDDVFLTEALTREAIKRLEAIRTNPREAG
                     KPFYLYMAHYALHSPLDERAYDKRFADAYKNPEDGHKWSRTEKHYSGLIEGMDKSLGD
                     IMKYLREHHLEKNTVLVFMSDNGGLAISGRLGNEEANYPLSFGKGSCMEGGIREPMIV
                     SWPGVTKGGSRCAVPVVIDDFFPTLLDIAGCRNVEVPQKLDGLSLVPLLKGGRFPEDR
                     PLLFHQPNNWGEGSRQAPQYTSSTALRQGDWKLIYRHLTQSFELYHLRKDIGEKENLA
                     SREPRKTREMAVVMGRLLRERKAQMPTYKKDNELGAPAGSSVPWPDQVKGNGF"
     gene            complement(6214..6960)
                     /locus_tag="PK077_05300"
     CDS             complement(6214..6960)
                     /locus_tag="PK077_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23750.1"
                     /translation="MTNIHPFTTAAACILTAGAALFTSSCQPASPESYARQFATAQHV
                     SVHGYNGRETLTSRVYADWGSMPANARRAMEEYIKGAEWKTMDYIRPQYFIQVDRSFW
                     AICIDNDGNMTGIIPFRNGKDARKMSVNNHFKMLVNTTDRAPALGYAILKNLAYADGL
                     RVSTRKADGLETPVPAPPAMKVVVPPKGAAPSAAPAAKSAATSVSEPGATEETPADDT
                     AGTEEEPSADSAAESEDTTTTDDAAVTDDF"
     gene            7182..>7511
                     /locus_tag="PK077_05305"
     CDS             7182..>7511
                     /locus_tag="PK077_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRN23751.1"
                     /translation="MSAFIISLPALERNALILRDVADAAGCRIALALKGFSCWPCFEA
                     LSPWLDGCCASGLWEARLARRRFGKHVLTYSPGHAPEEIAELAEISDHLDFNSLTQWS
                     RFREEVMR"
BASE COUNT         1448 a         1934 c         2356 g         1773 t
ORIGIN      
        1 ctcaagggag tgggggagtt caggcatttc aacgattttt tctaccgccg cctgaaaccg
       61 ggagctcgcc ctttggccgg cggggaggat acggccgttt ttcccgcgga tgcccggcac
      121 atgggctggg aacgcgccga ccgaattaag aacgttttcg tgaaagggca gcgttttgac
      181 ctgccttccc ttctggggag cgacaccctg gctgagcggt atgcggccgg cgccgtcgtc
      241 ttgtcgcgcc tgtgccctac ggattaccac cgttttcatt ttccggtgtc cggggtgccc
      301 ggcccctgga cgaagctggg aggcccgctg gcaagcgtgt ctccgtattg cctgcgcggc
      361 cgtctggcgt ggttatggac taataaaagg aatttgaccc tgatccggtc agagttgtgg
      421 ggagaggtcg ccatgctgga ggtgggagct accggagttg ggttgattga agagacgtat
      481 gttccgggtg ttttctccgc aagaggggcg gagaaggggt attttgcctt tggaggctcc
      541 acggtaatgt gcttttttga accgggaaga gtcagtttgg cttccgattt gttggaaaag
      601 acggaagaag gcttggagct gtttgcccgg cagggagaca tgatgggcac ggcagcgaca
      661 tagcctccgg gctggcgtgc ctgtcccgca atgttttgag tagggctctt tcctgacgga
      721 atgccgggga tggattccgg cgtttcaggg cggattttcg gattttttga ttttccgaag
      781 tgccaggcgg gttatttcat ttccgcaatg gcgcgggcca tatccggagc cttgaaggta
      841 gaggagcctg ccaccagcac atccgctccg gccagagcgc attgagcggc attgttttgg
      901 ccaattcctc cgtccatttg aatgaggaag gaggcgcggt tttccgtgcg ccattcggcg
      961 gctttttcca ccttgtccaa tacggaaggc atgaaggact ggccgccgaa gccaggcact
     1021 acggacatga ccagcagcag gtctatttcc gccaggtaag gttctacggc ttcaaaggat
     1081 gtggccgggt tgagagccag gccgtttttt actccggctt cccggattcg cccgagtgtg
     1141 ttgcgaatgt agtaggagtc tcccagttcc acgtgaaagc agatgaggtt cacacctgcc
     1201 ctgacgaagc ggtcaaagta gtggtccggc cgttctatca tcaggtgggc atccagaaag
     1261 cggtccggcc ccaccgcttt tctgacggcg gcgcagattt ccgggccaaa ggagatgttg
     1321 tccacaaagt ggccgtccat gatgtccaga tgcagccagt cggcaccgga gtcaaaggct
     1381 cgccgcgctt cctccccaat gcgggagaaa tcagcggcca gcagggacgg ggagattatg
     1441 gtcatggctg agggaggctt ttcgggatgt cagttggcta caatgttgac gaggcggccg
     1501 ggtacgacaa ttaccttgcg cacagtgacg ccgtccagga aggttttcac cttggcggaa
     1561 gctagggcct gtttttccag ttcttcccgg gagatgtcct tgggcacttc cagtttgtcc
     1621 ctcagcttgc cgttcacctg gatgaccatg ggaacggtat tttcctccag cagggcttcc
     1681 tcccattcag gccagctttt ctggcaaagc tgcgtctggg ccagagccgg gaaggctgtt
     1741 tgcaggcggc tgtgtatttc ttccgtaagg tggggggcaa agggattcag cagagtcagg
     1801 aaagcatcat agtcctgaac gtccaccaca tccgcggagg tcatggcatt ggtgcattcc
     1861 atcattttcg cgatggcggt gttgaaggac atgccattga tgtcctccgt cactttttta
     1921 atggttttgt gcaattcctt gcgtacggca agcaccccgg cttccggagc gttctccacc
     1981 agtttggagt tgatttccca ttctccttcc tgattttccc ggaaggccac gcgccagacg
     2041 cgggccagga agcgggaaat gccttccacg cctttggttg cccagggttt cacgtccttg
     2101 agagggccca tgaacatttc atataaccgc agggagtccg cgccatattc gcggacgatg
     2161 tcgtccggat tgacgacgtt gccgcgggat ttggacattt tctggccgtc ttcccccagg
     2221 atgagcccct ggttgacgag tttctggaag ggttcattgg tgctgaggta gcccaggtca
     2281 aagagtacct tgtgccagaa gcgggcatag agaaggtgga gcacggcgtg ttccgtgccg
     2341 cccacgtaca ggtccacgcc tccgggggag ccggcggaac ccatccagta ttgttccgct
     2401 tcccggctga cgaagcgttc cgtgttggcc ggatccaggt aacgcaggta gtaccagcag
     2461 gagccggccc attggggcat ggtgtttgtt tcccggtggg ccgtgggcgt gtaggctatc
     2521 cattccgcag ccttgaccag cgggccgcgg gggtctcccg tgggggcgaa gtcgtccaga
     2581 tccggctgga gtacggggag ttcgctttcc ggcagggcgc ggtggtgacc gttttcccat
     2641 acgatgggga atggttcccc ccagtaacgc tggcgggaga agagccagtc gcgcagcttg
     2701 tagtttactt tgcggcgtcc cttgccgttt tcttccagcc agaggatcat tttttccttg
     2761 gcttccggag taggcagccc ggtcaggaag ccggagttca cgctggaacc ttcataaccg
     2821 cagaagccgc gccagtccat gtcttcactg ggcggctgaa ctacctggag aatgggcagg
     2881 ccgaagacct gggcaaaggc gaagtcgcgt tcgtcatggg cggggacggc catgatggct
     2941 ccggttccgt agcccatcag cacatagtcc gcgatccaga cgggaatttg ttttccattg
     3001 accggattga cggcataggc tccggtgaag acccctgttt tttccttgga gaggtccgtg
     3061 cgttccaggt cgcttttgga ggcgcactgg gcgcggtatt gttccacagc atgtttctgt
     3121 tccggcgtgg tgacggtatc taccagaggg tgctccgggg aaagaaccat gtaggtggct
     3181 ccgaagaggg tgtccggcct ggtggtgtaa acagtaatcg tttctccgtc cagggtgaag
     3241 tccacttccg caccttccga tttgcctatc cagttgcgct gaagcagttt aatggattcc
     3301 ggccagtcca ggggctccag ctcgtcaatg aggcgttccg cgtagtccgt gatgcgcagc
     3361 atccactggc gcagggggcg gcgttccacg cggtgtccct tggatttcca ttcctccact
     3421 tcctcattgg caaggacggt tcccaggtcc ggggaccagt tgacggcagc ttccgctacg
     3481 taggccagcc ggcgctggtc gatttcttcc cggctcaatc cctgttcctc cagttcggaa
     3541 acggggcgcg cctttttcag ttccttgttg tagtaggagt tgtaaagctg gaggaatatc
     3601 cactgggtcc aacggacgta ttcatggtcc gttgttgcta tttcccggtc ccagtcgtaa
     3661 gagaacccca gcattttgag ctggcggcgg aagttgtcta tattccggaa ggtagtgacg
     3721 gaggggtgct gtccggtttt aatggcgtat tgttccgccg gaaggccgaa ggagtcccac
     3781 cccatgggat ggagcacgtt gaagccgttc atgcgtttga aacgcgctac aatgtccgtg
     3841 gcggtgtacc cttccggatg gcccacatgc aggcccgccc cgctggggta ggggaacatg
     3901 tccagcacgt agtatttggg tttggacgca tcaaagccct cttctccggg gttgtttact
     3961 ttaaaggttt tcctctcgtc ccagatctgt tgccatttag gttcaaaaac gtcaaaggga
     4021 tatggtttct tgcgctcgga catgacggga gggaatgtat actccgcccg cgggtcaatc
     4081 aagcctaaac caggcggaat gcaggcgctt ccaatgctcc gggggcatga tgccgttttt
     4141 cggaaaaaag gggaaaggta agaagacgtt tgcgcgtatt tcttaactga aaggtttttg
     4201 ttgattgcga ccatgaagtt tacttcctgt attatgtctg ccgtctgcgg agcattgttc
     4261 ctgtttgccg gggcatccgc tctgggcaat ggttccaacc ctgtttccgg aaagaagccc
     4321 aatatcatcg ttttcctggt agatgacatg ggatggcagg atacttccca cccgttctgg
     4381 tccgacagcc agggcaaccc gaagaaaacc tttttgaaca ggcgctaccg gacgccgaat
     4441 atggagaagc tggcttccca gggcatgacg tttacggatg catatgctca tcccctttgc
     4501 actccttccc gggtgagcct gatgtccggc atgaatccgg cgcggcaccg cgtgacctgc
     4561 tgggtacggg aacagaacgg gacgacggac gccaacagca ggagcctcct gcctccggac
     4621 tgggcgttga acggccttca gcctataggc actcccgcca ggggaacgac aaaacgcccc
     4681 atttccgggg aggatatgcg ctatcacatg acgcgtcctt ttgccacggc ggctacactg
     4741 ccagagatgc tgaagaagtg cggttatgtt accgtccatt gcgggaaggc ccatttcggc
     4801 acgcagggaa ctccgggttc caatccgttg aatatgggat ttgattataa tatcgcaggt
     4861 acggagatcg gccatccggc ggattaccgc ggttccaggc attacggaaa ggggtttaac
     4921 catgtgcgcg gactggatga gaataattat taccaggacg atgtattttt gacggaggcc
     4981 ctgacgcggg aggccattaa acgcctggaa gccatcagga ccaatcccag ggaggctggc
     5041 aagccctttt atctgtacat ggcccattac gctttgcatt ctccgctgga tgagcgcgct
     5101 tacgacaaga ggtttgcgga tgcctacaaa aacccggagg acggccacaa gtggtcccgg
     5161 acggagaagc attattccgg gctgatcgag gggatggaca aaagcctggg ggatatcatg
     5221 aagtatctca gggaacatca tctggaaaaa aataccgtac tggtgtttat gtcggataac
     5281 ggaggcctgg ccatctccgg cagactgggc aatgaagagg ccaattaccc tctttccttc
     5341 ggcaaggggt cgtgcatgga aggcggtatc cgggagccta tgattgtttc ctggccgggc
     5401 gtgacgaagg gcggttcaag gtgtgccgtc ccggtggtta ttgacgattt tttcccaact
     5461 cttctggata tcgccggatg ccggaacgta gaagttccgc agaagcttga cggcttaagc
     5521 ctggttcctt tgctcaaagg cggccggttt cctgaagacc gccccctttt gttccaccag
     5581 ccgaataatt ggggggaagg cagtcggcag gcgcctcagt atacttcttc caccgcattg
     5641 cgccaggggg attggaaatt gatttaccgc cacctgaccc agagctttga gctgtaccat
     5701 ttaaggaaag atatcggcga gaaggaaaac ctggcttcca gggagccgcg gaaaaccagg
     5761 gaaatggctg ttgtcatggg caggttgctc cgggagagga aggcgcagat gccaacctat
     5821 aagaaggaca atgagctggg cgctcctgcc gggagttccg ttccgtggcc cgaccaggtg
     5881 aaggggaatg gtttttgatg atccgcaata acgtttttcc aaagtttttt cataaccccc
     5941 ggcaggattc acgtcccgcc gggcggaccg ggtcgaggct ggggtgccct cacttccttt
     6001 tccagtagtt ttttctgtcc ggtataggaa tggtgtgtgt tttcttttcc ggatgcaggg
     6061 tcatcgttct tcgtgaatga aggccgcagc ttgtttcgga gtaaggggag gaatgagcaa
     6121 gggaactggt tcgggaggga taaaaacgcc ccgacggata tggcgtcggg gcgtgacaga
     6181 ggttaaataa cgaagttccc tgaaagggag ggtttaaaaa tcatcggtga cagcggcatc
     6241 atccgttgtc gtggtatctt cgctttcagc agcgctgtcc gcagagggtt cctcttccgt
     6301 tccggcagta tcgtcggcag gagtttcttc cgtagcgcct ggttcagaaa cggacgttgc
     6361 tgcggatttg gcagcgggcg ctgcggacgg agcagctccc ttgggcggaa caacgacttt
     6421 catggccgga ggggcgggaa cgggagtttc cagaccgtca gccttgcggg tgctgacgcg
     6481 gagtccgtca gcataagcca ggtttttcag gatggcatag cccagggcgg gagcccggtc
     6541 cgtcgtgttg accagcatct tgaagtggtt gttgacggac atcttgcggg cgtccttgcc
     6601 attgcggaag ggaatgatgc ctgtcatgtt gccgtcattg tcaatgcaga tcgcccagaa
     6661 agagcggtct acctggatga agtactgcgg gcggatgtag tccatcgttt tccattcagc
     6721 gcccttgatg tattcctcca tggcgcggcg cgcattggcc ggcatgctgc cccagtcagc
     6781 gtaaacccgg gaggtcagcg tttcacggcc attgtagccg tgcacggata cgtgctgggc
     6841 agtagcaaat tgacgggcat agctctcggg agaggcaggt tggcatgagc tggtgaagag
     6901 cgctgctccg gcggtcagga tgcaggctgc tgcagtagtg aagggatgaa tgttagtcat
     6961 ggcaatcgca atggttacgt tataaaagag gaaaatacat cccatttcaa ggaaaagaat
     7021 gcaggaggta ccgttttgtg ccgttttttg acgtttccgt ttgatgcggg gggctggcgg
     7081 gagtggggaa ggcgttctca tgcccgtctc gcaggtatga tggtaaatta ctaaaatttt
     7141 tctcgcaaat cgttctgaat cgcttaggtt gtcgttgctt tatgtccgcc tttatcatca
     7201 gcctgccggc cctggaacgc aacgctctta tcctgaggga tgtggcggat gccgcagggt
     7261 gccgcatcgc tctggcgctg aaggggtttt cctgctggcc ctgttttgag gccctgtccc
     7321 cctggctgga cggctgctgc gcttccggcc tgtgggaggc gcggctggcc cggcgcaggt
     7381 tcgggaagca tgtgctgact tattctcccg gccatgcccc ggaggaaata gcggagctgg
     7441 cggagatttc cgaccacctt gattttaaca gcctgacgca gtggagccgt ttccgggagg
     7501 aagtgatgcg g
//
LOCUS       DAOMSU010000091         7453 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_766_length_7453_cov_3.491214, whole genome shotgun sequence.
ACCESSION   DAOMSU010000091 DAOMSU010000000
VERSION     DAOMSU010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_766_length_7453_cov_3.491214"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="PK077_05310"
     CDS             <1..1016
                     /locus_tag="PK077_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRN23752.1"
                     /translation="GIIMRTVAQGQRYRHFVRDLAMLLEQWYSVEEKKEANPAPVCVY
                     KEPDLIERTARDFLTDTVDNIICDNAETTEHMRAIAGKISRRAKRHIQHFPVRTPIFE
                     ELGIEKQINEAFQRQVLLPCGGYLVIDETEALIAIDVNTGRNKGTKDLDKTILDTNLE
                     AAYEIARQLRLRNIGGLVVVDFIDMRHRKDQQAVYKAMKDRLKRDKAKTQVLQITPIG
                     LMEMTRQRLNESLRESVHDPCPYCGGRGRVKSVMTMSVEIQRQIYACIQKYRDTIGDM
                     VIVVNSEVLSRFKNEDASLLMELERQHSGRLIFRGDHNQHRESFAIYDARYDNKPLYQ
                     SGQ"
     gene            1082..>1529
                     /locus_tag="PK077_05315"
     CDS             1082..>1529
                     /locus_tag="PK077_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRN23753.1"
                     /translation="MNNIPTSPNDPVNTDILLVAEDRIKGFRRRPFHAISEQCGHPVP
                     VVIQRLKAMMEAGVVRRVRQTLLATKLAQGALVAWSLPEEKLDAAFDWMKEQDPFTGH
                     VVLRNTDAANPGANYRLWTTVKVPVGCGTPEEHCGILKRHIGADGYV"
     assembly_gap    1530..1549
                     /estimated_length=20
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1550..2195
                     /locus_tag="PK077_05320"
     CDS             <1550..2195
                     /locus_tag="PK077_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRN23754.1"
                     /translation="GVGHMRRRELRPGEKTPEPAPMQETKTVSLSELEWKVLLSVKEQ
                     LRPEEMVEDPWSLRAAREGLGTEEYCRTAEELDRKQVIGRFATFLEHVRAKTDDGPVT
                     KYNGLFHWTVPSGMEIRAGAECGRHICMTHCYWRTGGDVFGGAQIMGVVHGLEKDAVL
                     EHKAAIDRHLEASGIPILHTAVFWGERSEIKPSEISPELYRKWMEEVKKAPVLQ"
     gene            complement(2665..3708)
                     /locus_tag="PK077_05325"
     CDS             complement(2665..3708)
                     /locus_tag="PK077_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23755.1"
                     /translation="MKALSVIMVLAGLSSLVPSMAQNAQNPQRLRARVAAPTIKNGRP
                     TDIYILSANGPTVQFVESRESQEVLQQMASAFKTLYIFETDDFVDAKVAMENRKYQEA
                     RNKFHALVNKYASTLSIKDSLSARAAVYELECAMRMMDWAGVKGLAAKFPVRGANLSP
                     SAQNDLEVAKIMALIPDKDWNGVKSRAGSFLATKKNATRLQQARMKYALGAAAMVAQD
                     WNKALDYFAEALVLLHGSDEELASACVARSLDAYLCMPDVVKFFENPVVSSAVESRKN
                     NPEAVIPDSRMKSRPAPVKEAAALYRLHELMFPDRKLPVKYDGFAVSYKHPAAVAPAK
                     APDQAPAQPQKKQ"
     gene            complement(3735..6968)
                     /locus_tag="PK077_05330"
     CDS             complement(3735..6968)
                     /locus_tag="PK077_05330"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN23756.1"
                     /translation="MIKSNYTTALAAGLVSVLSIGAVLPSGAQHGPRDYQRTALTAIK
                     EGKWQEALDAVDRCIRVYEPRIKMLGLDDGFGWFYYQKGVCLAQLKNYKEAVEAFKAC
                     YTKFPSAKNQLVKMALFREGENYCRLGDFAKGAELLEKFLKEYRSDPVARNVNAGEVQ
                     GLLAQCYFKMSPPAFEKGMENLTSCVTSRYKGRRITDAVITNGFLAMVDAAIKTGKCS
                     ETVKFVENYPSVMNISPTRVALYTPRLVSYVAEVLEKSRSLLQDGKQKESEDYASLAM
                     VLMGLLPDQSGVMADANYSLDRLGRANGAVPGVTDFSHTLDRAKVTALIDQFNKMKEE
                     GKVMDAFTFSFMGNQALVHGSQRVARAAYQLINESYPDAPGREDNLYYLAMTTWQLGE
                     ADKGGELVAQHLKEFPNSKYAPMLNTLSLEGLLKEKKFDLCVQQADKVMELHKDDPTH
                     KFYELALYCKGASLFNLGAADASRYKEAVPVLERFVKEYRDSTYLKTAMYLLGETYTN
                     LGNTDEAIRSFTNYIARFPDKGEANMAAVLYDRAFNYLNRKNPGDEELAAKDAKEIVD
                     NFKDHRLFPYANNLLANLYAGSKEHEQEAEGYFLAALESAKKLGDKRPAAEAVYNLFI
                     NATKKPLPVEPKEAVETARTARRDEVKKWYDEYWKDSDQPGSRYSLQLAAAAMEFFKD
                     DKEMFDPASVKMQEIIVREGKKDDPKMTVLLEEAVNSYTKTYMAGNQALGRNLDANAM
                     RNHFYRFPGVDNDKDKTLSAMLRMAVIAQTQERYEKAPVETDEQRAEKAALEGLVKQL
                     FVELKRDFKPSDLPPYTLVKLGMHLAGTSQPEESIAYFDEILDPSEPDPVRKKARING
                     MSKYRKNAVFGKAVALGRSKDNAKVDTAIKMMRDELSKEESSSNPDRKAMEDAQYNLV
                     KFTSARQDWPAVIAAADKYRENKTYKKNLPEVLYLQGEAYLKQNELDKALINFMNITG
                     TYKGLVKWSAPAVLAQMDTLWKRNTMSQGAGKQPSDRYVAWKAGSQYVQLLDTPANRK
                     KMTAEDSALVNEVKDKTAKFGSDPAVSQERADIAAYEAAVRAAKGQK"
BASE COUNT         1630 a         2158 c         1985 g         1660 t
ORIGIN      
        1 tgggcatcat catgcgcacg gtggcacagg gccagcgcta ccgccacttt gtccgcgacc
       61 tcgccatgct gctggaacaa tggtacagcg tggaagaaaa gaaggaggcc aatccggccc
      121 ccgtctgcgt gtacaaggaa ccggacctga ttgaacgcac ggcacgggat ttcctcacgg
      181 acaccgtgga caacatcatc tgcgacaacg cggaaaccac ggaacacatg cgcgccattg
      241 ccggtaaaat ctcccgacgc gccaagcgcc acatacagca cttccccgta cgcacgccta
      301 tctttgaaga actgggaatt gaaaaacaaa tcaatgaagc tttccagcgc caggtgctcc
      361 tgccctgcgg aggctatctg gtgattgacg aaacggaggc gctgatcgcc attgacgtca
      421 acaccggacg caacaaggga accaaggatc tggacaagac cattctggat accaatctgg
      481 aagccgccta tgaaatcgcg cgccagctcc gtctgcgcaa catcggcggt ctggtggtgg
      541 tggactttat cgacatgcgc cacaggaagg accagcaggc cgtttacaag gccatgaagg
      601 accgcctgaa acgggacaag gcaaaaaccc aggtactgca gatcaccccc atcggcctga
      661 tggaaatgac gcgccagcgg ctcaacgaga gcctccggga atccgttcat gatccgtgcc
      721 cgtactgcgg aggcagggga cgcgtcaaat ccgtaatgac catgagcgtg gaaatccagc
      781 ggcaaattta cgcctgcatc cagaaatacc gggacaccat cggagacatg gtcatcgtcg
      841 tcaactcgga agtgctctcc cgcttcaaga atgaagacgc cagcctgctc atggaactgg
      901 aacgccagca cagcggaagg ctgatcttcc ggggcgacca caaccagcat cgtgaatcct
      961 tcgccattta cgatgcccgg tacgacaaca aacccctcta tcaatccggg caatagcctg
     1021 aaaatcaaat ccggctttcc ggggccgggc gacactctcg cccggccttt tttacgccgc
     1081 catgaacaac atccccactt ctccgaacga tcccgtcaac acggacattc tgctggtggc
     1141 ggaagaccgc atcaaaggat tccgccgccg gcccttccac gccatttccg aacaatgcgg
     1201 acatcctgtt ccggtagtta tccagcggct gaaagccatg atggaggccg gagtagtgcg
     1261 ccgcgtgcgc cagacgctgc tggcgaccaa gctggcccag ggagccctgg tggcctggtc
     1321 cctgccggag gaaaaactgg atgccgcttt tgactggatg aaggagcagg acccttttac
     1381 gggccatgtg gtgctccgca atacggatgc ggccaacccc ggcgccaact acaggctctg
     1441 gaccacggtc aaggtgcccg tgggatgcgg tacgccggaa gaacactgcg gcatcctgaa
     1501 acgccatatc ggagcggacg gctatgttcn nnnnnnnnnn nnnnnnnnng ggggtggggc
     1561 acatgcgccg cagggaactc aggcccggag aaaaaacccc ggaaccggct cccatgcagg
     1621 aaacgaaaac cgtttctctc tccgaactgg aatggaaggt gctcctctcc gtcaaggaac
     1681 agctcaggcc tgaggaaatg gtggaagatc cctggtccct ccgtgccgca cgggaaggac
     1741 tgggaacgga agaatactgc cgcacggcag aagaactgga ccggaagcag gtcatcggcc
     1801 gtttcgccac ttttctggaa catgtgagag ccaaaacgga cgacggccct gttaccaaat
     1861 acaacggact gttccattgg acggttccct ccggcatgga aatccgggca ggagcggaat
     1921 gcgggcgcca catctgcatg acccattgtt attggagaac gggcggagat gtttttggag
     1981 gagcgcaaat catgggtgtg gtgcacggat tggaaaaaga tgccgttttg gaacacaagg
     2041 cggcgattga ccgccatctg gaggcctccg gcattcccat cctccacacg gctgttttct
     2101 ggggcgaacg ttcggaaatc aagccttccg aaatttctcc ggaactgtat aggaaatgga
     2161 tggaagaagt aaaaaaagct cccgttctgc agtaactccc ttctccacgc ccgcctccgg
     2221 cggtcttccc ccggtcgaac agcttttccc tccgaactcc cgcgaatgcc gtcacgaagc
     2281 cacaagcatt tttaatgccg caactgagat gccggctgtt tcccctccct ccgtcctgct
     2341 gtaccgctta gttccctgtt cccagcctac agcggcttaa taccgttttt ccggacgttc
     2401 caggatcttt gatacatctt cctgtcaatt caggaaacgg gactgaagaa atccatttca
     2461 agccttcagg gagacgcacg tcccaatctg atgagatatt catgttctcc tttgctacga
     2521 cgctgtttcc tttccggata caccgggaga ggcttctgcc tgcagaaaca agaagaaggc
     2581 aacataaggg acgcctcccc gtaaggggga ggcgtccctt attaaagaaa tatcggccgg
     2641 cgaacaacct gccgcgaagc tttatcactg ctttttctgc ggctgtgcgg gagcctggtc
     2701 aggagccttg gcgggagcaa cagcggccgg atgcttgtag gaaaccgcaa aaccgtcata
     2761 cttgaccggc agcttcctgt ccgggaacat cagttcatgc aaacggtaca gggcggccgc
     2821 ttccttaaca ggagcgggac gggacttcat ccgtgaatcc ggaatcacag cttcgggatt
     2881 attcttcctg gattccacgg cagatgagac gacgggattc tcaaagaatt tgacaacatc
     2941 cggcatgcac aggtaggcat ccagggagcg ggccacgcag gcagacgcca gttcttcatc
     3001 ggaaccatgc agcaaaacca gggcttccgc aaaataatcc agcgccttgt tccaatcctg
     3061 ggccaccatg gcggccgcac ccagggcata tttcatccgc gcctgctgga ggcgggtggc
     3121 attctttttg gttgccagga aagaaccggc acggcttttt acgccgttcc agtccttatc
     3181 cggaatcaag gccatgattt ttgccacctc caggtcattc tgggcggaag gggacagatt
     3241 ggcccctctg acgggaaact tggccgccaa ccctttgact ccggcccaat ccatcatgcg
     3301 catggcgcac tccagttcat agacggccgc ccgggcggac aggctgtcct tgatggaaag
     3361 cgttgaggca tacttattca ccagagcgtg gaatttgttg cgggcttcct ggtacttccg
     3421 gttttccata gccaccttgg catccacaaa gtcgtccgtt tcaaaaatat acagcgtctt
     3481 gaaggcgctg gccatctgct gaaggacttc ctgggattcc ctgctctcca caaactggac
     3541 cgtggggcca ttggcggaaa gaatgtagat atccgtcgga cggccattct tgatggtcgg
     3601 tgcggcaact ctggcgcgca ggcgctgggg attctgggca ttctgggcca tggacggaac
     3661 cagggaggac agtccggcca ataccattat tactgaaagt gctttcatat gattgattac
     3721 taaacctctt gttattattt ctggcccttg gcggcgcgta cggctgcttc ataggcggca
     3781 atgtccgccc gttcctggct gacggcagga tcggaaccga acttggccgt cttatccttc
     3841 acctcattga ccagggcgct gtcctccgcc gtcatcttct tgcggttggc gggagtatcc
     3901 agcaactgca cgtactggct gccggccttc caggcaacgt acctgtcgga aggctgcttg
     3961 cccgcaccct gggacatcgt attcctcttc cacagcgtat ccatctgcgc cagcacggcg
     4021 ggggcggacc acttcacgag ccccttgtac gtacccgtga tgttcatgaa gttaatcaac
     4081 gccttgtcca gttcattctg cttcaggtag gcttcaccct gcaaatagag gacttccggc
     4141 agattcttct tataggtctt gttttcgcgg tacttgtcgg cagcggcaat gacggccggc
     4201 cagtcctggc gggcggaagt gaactttacc agattgtact gggcgtcttc catggctttg
     4261 cggtccgggt tggagctgga ttcttccttg ctcagttcat ccctcatcat cttgatggcg
     4321 gtgtccacct tggcgttatc cttgctgcgc cccagagcta cggctttccc gaagaccgca
     4381 ttcttgcggt acttggacat gccgttgatg cgagccttct tacgcaccgg atcaggttcc
     4441 gacgggtcca ggatttcatc aaagtaagcg atgctttctt caggctggga agtgccggcc
     4501 aggtgcatgc caagcttcac aagcgtgtac gggggcagat cggaaggttt gaagtcgcgc
     4561 ttcagctcca cgaagagctg cttgaccaaa ccttccaagg cggctttctc ggcacgctgt
     4621 tcgtccgtct ccacaggagc cttttcatac cgttcctgag tttgggcaat aacggccatg
     4681 cgaagcatgg cgctcagcgt cttgtcttta tcattgtcca cgccggggaa ccggtagaag
     4741 tggttgcgca tggcattggc atccagattg cggcccaggg cctgattgcc ggccatgtac
     4801 gtcttggtat aggaattgac ggcttcttcc agaagaacgg tcatcttggg atcgtccttc
     4861 ttgccttccc tcacaataat ttcctgcatc ttgacggatg ccgggtcaaa catctccttg
     4921 tcatccttga agaattccat ggcggcggcg gccagctgga ggctgtagcg gctgccgggc
     4981 tggtcgctgt ctttccagta ctcgtcatac catttcttga cctcgtcccg gcgcgccgtc
     5041 ctggccgttt ccacggcttc cttcggttct accggaagag gcttcttggt agcgttaata
     5101 aacaggttgt acacggcctc cgcagcggga cgcttgtcgc ccagcttctt ggcggactcc
     5161 agagcggcca ggaaatagcc ttccgcttcc tgctcatgtt ccttgctgcc ggcatacaga
     5221 ttagccagca gattgttggc atacgggaac aggcggtggt ctttgaaatt gtccacaatt
     5281 tccttcgcat ctttcgcggc gagctcttcg tctccggggt tcttgcggtt taggtagttg
     5341 aaggcccggt catacaatac ggcggccata ttggcctcac ctttgtccgg gaaacgggca
     5401 atgtaattgg taaaggaccg gatggcttca tccgtattgc ccaggttcgt gtaggtttca
     5461 ccaagaaggt acatggccgt tttcagataa gtgctgtcac ggtattcctt cacgaagcgt
     5521 tccagcaccg gcaccgcttc cttataacgg gaggcgtcgg cagcccccag gttgaacagg
     5581 gaggctcctt tgcagtacag ggccagttca tagaacttat gggtggggtc atccttatgc
     5641 agctccatga ccttgtccgc ctgctgaacg cagagatcga atttcttttc cttcagaagc
     5701 ccttccaaag acagcgtatt aagcatggga gcatacttgg aattggggaa ttccttcagg
     5761 tgctgcgcca caagctcgcc tcccttgtcc gcttctccca gctgccaggt ggtcattgcc
     5821 agataataca ggttatcctc cctgcccgga gcatccgggt aggattcatt gataagctgg
     5881 taggcggcgc gggcaaccct ttgggaacca tgcaccaagg cctggttgcc catgaagctg
     5941 aacgtaaaag cgtccatgac ctttccttcc tccttcatct tgttgaactg gtcgatcagg
     6001 gctgtcacct tcgctctgtc cagcgtatgg gaaaaatccg tcacgccagg cacggccccg
     6061 ttggcacggc ccagacgatc cagggaataa ttggcatccg ccattactcc ggactgatcg
     6121 ggaagaaggc ccatcagcac catcgccagg gaagcataat cttcagactc tttctgcttt
     6181 ccatcctgaa gcagggaacg ggatttctcc agcacttccg caacgtagct caccagccgc
     6241 ggagtgtaca aagccacacg cgtggggctg atattcatca cggaagggta gttttccaca
     6301 aacttcaccg tctcactgca tttgccggtc ttaatagcgg catccaccat cgccaggaag
     6361 ccattggtaa taacggcatc cgtaatgcgg cggcccttgt agcgtgacgt aacgcaagag
     6421 gtaaggtttt ccattccttt ttcaaaagca ggcggggaca tcttgaaata gcattgggcc
     6481 agaagcccct gcacttcacc ggcattgacg tttctggcga caggatcgct gcggtattct
     6541 ttcaagaatt tttccagcag ctccgcgccc ttggcaaaat ccccaagacg gcagtagttt
     6601 tccccttccc ggaacagggc cattttcacg agctggtttt tagcgctcgg gaacttggtg
     6661 taacaagcct tgaacgcttc cacagcttcc ttgtaattct tcagctgggc cagacagacg
     6721 cccttctggt aatagaacca gccgaagccg tcatccagcc ccagcatctt gatacgaggt
     6781 tcataaacgc ggatgcagcg atccacggca tccagggcct cctgccactt cccttcctta
     6841 atggccgtca gggccgtgcg ctggtaatcg cgcgggccat gctgggcgcc ggatggaaga
     6901 accgccccga tactcagcac ggaaaccagg cctgcagcca gtgcagtagt atagtttgat
     6961 ttgatcatgt gtttaaaatt cgttgaaaca gaagagataa taaaacaggc gcaatatccg
     7021 tgcaaataca cggaatacac ctatctgtaa tggattgcgt cattttaaca agatgtgtca
     7081 aactctaaag aagaaagttt tatcttttta gtcatccttg caagaaagtt cttccatccc
     7141 gcgtttatcg ggagttggaa caggtcggcg ccgcccggcc ggaaaaatac tgctttttca
     7201 ataccctgct acacactctg cggccaaagg aatgcacgct ttccgccttc aaaagttttt
     7261 cggcggaaca cagcctactg ctccatcatg cccttaagga gcctcgaatc ccgtctgtca
     7321 agatgccaga ggcatattta atggcgcaaa catatcgtcc gcccaaccgg aattccattt
     7381 ttccggcatc cgcagccata ccggccttct ccccggcaac agctacccct cccctctcag
     7441 ttctcctttt tca
//
LOCUS       DAOMSU010000092         7440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_768_length_7440_cov_2.668653, whole genome shotgun sequence.
ACCESSION   DAOMSU010000092 DAOMSU010000000
VERSION     DAOMSU010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_768_length_7440_cov_2.668653"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..>563
                     /locus_tag="PK077_05335"
     CDS             140..>563
                     /locus_tag="PK077_05335"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HRN23757.1"
                     /translation="MAKKDYYEILGVSKDATDDEIKKAYRKLALKYHPDRNPDDPSAE
                     EKFKELGEAYEVLSDADKRAAYDRFGHAAFEQGGPAAGGGYAGGGFQDPMDIFAQMFS
                     GMGGFADMFGGAGRGGQKRSTKRPGSDLRYDLDITLEEA"
     assembly_gap    564..578
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <579..1286
                     /locus_tag="PK077_05340"
     CDS             <579..1286
                     /locus_tag="PK077_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HRN23758.1"
                     /translation="IERLVTCKTCHGTGARDGKEAFKSCPTCQGRGIITQQSGFFVQQ
                     STCPTCHGTGEIISDPCPVCRGEGRVREDSHITIRIPAGVATGSQLRIAGEGDAGVHG
                     GPTGDLHVFIDVKPHDIFQREGNDLSCTVPVPLSLAVSGGRLKVPTLEGAATIKLPEG
                     TQNGMIFRLRGKGVKALRGSDVGDMLVEVEVEIPSRLTKEQVDKLNAFASALDENRNQ
                     PACVEFADKAARYLKGK"
     gene            1309..2046
                     /locus_tag="PK077_05345"
     CDS             1309..2046
                     /locus_tag="PK077_05345"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008692.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HRN23759.1"
                     /translation="MARFFLPPSEWAAPAWELRGDEAHHAAKVLRLQQGDSCIVFDGC
                     GRAAHAVVAEPPRSSGVLLVPGEECPPSPPVAHLTLCQAVPKGTNMDLIIQKSVELGV
                     SAIVPLVTDRTIVRLNAREAEAKRQKWQRIALEACKQCGQNTLPKVALPVPFAEWLRG
                     GIPEGLNIIASLAPGVRPVREVLEAARSRSVRHASLLVGPEGDFTDRETAMALEAGFV
                     PVTLGPIVLRVETAAFFGLAAMRYALD"
     gene            2067..3059
                     /locus_tag="PK077_05350"
     CDS             2067..3059
                     /locus_tag="PK077_05350"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRN23760.1"
                     /translation="MFKNIIFDWSGTLVDDLALTLDASNYVFSQYGKPCMNRDEFRAE
                     FQLPYPDYYARVLPHADLDELEDHFRYAFRVSNAPVEVLPNAREFLEFCRARGVRCFI
                     LTSVDAKEFDTQCRELGMMEYFEAIHAGIRHKDAHIHTLLAQHGLHAHETAFIGDMQH
                     DVETAHHAGITSIAVLTGYNDAAQLSKAKPDMIVPDLLVLRTLMRRYALPSDTQDSIN
                     INGLELDTFIGVPDEERASMQTLKADITFYPEEALSGLNDDFSRTVCYDSMARALRAE
                     AMARPRKLVETLAEDMGKVCLKEFGARHVVVTLRKFILPRTDSVSVTVHVSRHR"
     gene            complement(3072..4823)
                     /locus_tag="PK077_05355"
     CDS             complement(3072..4823)
                     /locus_tag="PK077_05355"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRN23761.1"
                     /translation="MLTGPCPAADYPERTERTQSAGNHVWHIDPDKGNDGNPGTTPST
                     AWKSMAPANRLIMARGDTLVIHPGEHAVSLALMGEGSKQAPVTIRFMPGRHIFKHGAL
                     MTGKPQISNTNDAPNEPKAMAVRLMEAKNIRLEGKPGATDILLEGKAIFVCMEHVENV
                     SLNGLGFDYLHPTMGEFLVTEVEGDTMKATIPDGILYTVKDGNLTWHGPGWEFRMGGY
                     SKVFDSASGTFQGRFDPGKTVIRELSPGKISITFKEGSPTMKPGQSYQNRNTRRDCCG
                     FFQYRSKNILWNNCHIYYMHGMGVVSQFCENIMFSRLNIAPRPRSLRTSSSWADNLHF
                     SGCRGKIIVKDCVLGASHDDAINVHGTHLRIVGRPAPNKITVRFMHPQTFGFDAFAPG
                     DRIDYVSSNTLVPYASNTVSGVNQLNEKEIELTLQHPNPENIQPDDVVENVTWTPSVH
                     ISNTVCRHIPTRGFLLTTRKPVLVERCRFEKTGMPAILVEDDASGWYESGVVRNMTIS
                     RNTFIQCGEAVIQIVPHAPRPEGEVHRNITITGNTFDLKNGTAIRTRHTGSVKAEKNT
                     FTKDGKKIPEEKAVDIR"
     gene            complement(4947..5882)
                     /locus_tag="PK077_05360"
     CDS             complement(4947..5882)
                     /locus_tag="PK077_05360"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HRN23762.1"
                     /translation="MTQAQTITSKAKSNLAFALIDLPEEERRHMAEFYAFCRTVDDIV
                     DEPGMTPRERHDALNRWVEVINGGEGLELTELEEDIIALIQDLELDTTPMLHLIEGCR
                     SDICQQQPRSRDELLDYTYCVACCVGLTSARIMGAGEASYPYAIALGHALQMVNIIRD
                     VAEDCGKSNRIYLPKEDMDRFGYTLEDLRCRVYSPRLQELLKYEADLAETFFAEAERE
                     YNLLSPEDKAALIPAQAMALIYHTILDKMKASGFRIFDIRYRVNTFHKLWLLFRTKLD
                     IDPQSYYDKSKAYYDKLVGFLSRPGRKDEKQGPPR"
     gene            complement(5922..7205)
                     /gene="lepB"
                     /locus_tag="PK077_05365"
     CDS             complement(5922..7205)
                     /gene="lepB"
                     /locus_tag="PK077_05365"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRN23763.1"
                     /translation="MNSPHKSILAALYDCFPAANAVLVFLLDHPLAWFTPKWRRKGRM
                     ALKAVRRYINYNRDLLPTDRLAEFEESRDLLKTALYRGDRQQAETITAKLESTLESIP
                     GAIPSALAENVEVLFVILAIFLGLRCYVVQPFRIPTGSMQPSLNGIRALPQEGEPTLM
                     QKIGDMILHGGSYVHETASKEKKIVRFEPATKYLLLTVTNVIFDDGSKLEIPAAEAET
                     RRYFLNQEPRFEDERHTPFRSYLPGDTIVNARFDAGDLIVVNKMAYHFRKPERGEVFV
                     FDTRGIEGIANKGSSTGQEGGTHYVKRLCGIPGDTLSIQDSQLIVNGKPATERTIQRV
                     ASGKPPYQPCGYVALPAPLSLLDGRAYITEGGTVHLSNDSKRPYLREYVALGDNSTRE
                     NSFDSRYWGPVRQYNIVGPASFCLWPFTSHWGLIP"
     gene            complement(7261..>7440)
                     /locus_tag="PK077_05370"
     CDS             complement(7261..>7440)
                     /locus_tag="PK077_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HRN23764.1"
                     /translation="LFHWVLAHGGAITGEHGIGLAKAKWFRKAVGEGAYHLHELVKSA
                     LDPKNLLNPGKMGLP"
BASE COUNT         1436 a         2012 c         2277 g         1700 t
ORIGIN      
        1 gccggaaggc accgttctgc gcgttatccg caaggggtat atgctgaaag acaggcttct
       61 gcgcccggcg aacgtggttg tcgcccatac tccggacccc gaaccgcaag tctgaccgtt
      121 tttttaacat tcacgccaca tggctaaaaa agattattac gaaattctcg gcgtttccaa
      181 ggacgctact gacgatgaga tcaagaaggc ctatcgcaag ctggccttaa agtaccatcc
      241 ggaccgcaac ccggacgacc cgtccgcaga agaaaagttc aaggagctgg gagaggccta
      301 tgaggtgctt tccgatgcgg acaagagggc tgcctacgac cgcttcggcc atgccgcctt
      361 tgaacagggc ggtcccgctg cgggcggcgg ttatgccggg ggagggttcc aggatccgat
      421 ggatattttc gcccagatgt tttccggaat gggcggtttt gcggatatgt tcggaggcgc
      481 cggccgcggc ggccagaaaa ggtccaccaa gagacccggt tccgatttgc gctatgattt
      541 ggacatcacg ctggaagagg ctgnnnnnnn nnnnnnnnat tgagcgtctg gtgacttgca
      601 agacctgcca cggtaccggg gccagggatg gaaaggaagc gttcaagtcc tgtcctacct
      661 gccaggggcg cggcatcatt acccagcaga gcggcttttt cgtccagcag tccacctgcc
      721 ccacctgcca cggcaccggg gaaatcatct cggacccatg ccccgtctgc cgcggggaag
      781 ggcgcgtcag ggaggattcc cacatcacca tccgtattcc tgcgggcgtt gccacgggca
      841 gccagctccg catcgccggg gaaggggatg ccggggtgca tggcggccct accggggatc
      901 tgcacgtgtt tattgacgtc aaaccgcacg atattttcca gagggagggc aacgacctga
      961 gctgcacggt tccggttccg ctttccctgg ctgtttccgg cggcaggctg aaagtgccca
     1021 cgctggaagg cgcggccacc atcaagctgc cggaaggaac gcagaacggc atgatcttcc
     1081 gtctgcgcgg caagggggta aaggcccttc gcggttcgga cgtgggggat atgctggtgg
     1141 aggtggaggt ggaaatcccc tcccggctta ccaaggagca ggtggataag ctgaacgcgt
     1201 ttgcctctgc cctggatgaa aatcggaacc agccggcttg cgtggagttt gccgacaagg
     1261 cggcgcgtta cctgaagggc aaataaggtc cgtttccctt tttcagacat ggcccgcttt
     1321 ttcctcccgc cttccgaatg ggctgctccc gcctgggagc tgcgagggga tgaagcgcac
     1381 catgccgcca aggttctgcg cctgcagcag ggtgactcct gcatcgtgtt tgacggttgc
     1441 ggccgtgcgg cccacgctgt ggtggcggaa cctccgcgca gctccggcgt gctgctggtt
     1501 ccgggggagg aatgccctcc ttctcccccc gtggcccatt tgacgttgtg ccaggctgtt
     1561 cccaagggga ccaacatgga tctcattatc caaaagtctg tggaactggg ggtttctgct
     1621 attgttccgc tcgtgacgga ccgcacgatc gtgcgcctga atgcccggga ggctgaggcc
     1681 aaaaggcaga aatggcagcg catcgccttg gaggcctgca agcagtgcgg ccagaacacg
     1741 ctgccgaaag tggcgcttcc cgttcccttt gcggaatggc tgcgcggagg aattccggaa
     1801 ggactgaata ttatcgcctc tctggcgccc ggagtgcggc cggtcaggga agtgctggag
     1861 gccgctcgtt cccgttccgt ccgtcacgct tctctgctgg tggggccgga gggggatttt
     1921 acggaccggg agacggccat ggcgttggaa gcggggtttg ttcccgtcac gcttggccct
     1981 atcgttctcc gggtggagac ggccgccttt ttcggcctgg ccgccatgcg gtacgccctg
     2041 gactgagttt tttcatacgc attcccatgt ttaaaaatat cattttcgac tggtccggca
     2101 ccctggtgga cgacttggcc ctgacgttgg acgcctccaa ctacgtcttt tcccaatacg
     2161 ggaaaccctg catgaacagg gatgaatttc gcgcggaatt ccagcttccc tatccggact
     2221 attatgcccg ggtgctgccc cacgcggatc tggatgaact ggaagaccat ttccggtacg
     2281 ccttccgcgt ttccaatgct ccggtggaag tgctgccgaa tgcccgggaa ttcctggagt
     2341 tctgccgcgc gcgcggcgtg cgctgcttta ttcttaccag cgtggacgcc aaggagtttg
     2401 atacccaatg cagggagctg ggcatgatgg aatattttga agccattcat gcgggcatcc
     2461 gccataagga tgcccacatt cataccctgc tggcccagca tgggctgcat gcccatgaaa
     2521 ccgcatttat cggggacatg cagcatgatg tggaaacggc ccatcacgca ggcatcacat
     2581 ccattgccgt cctgaccggc tataatgacg ctgcgcagct ttccaaggcc aaaccggaca
     2641 tgattgttcc ggacctgctt gttctgcgca cgctgatgcg ccgttacgcg ctcccttccg
     2701 atacgcagga ttccattaac atcaacggtc ttgaactgga taccttcatc ggcgttccgg
     2761 acgaggagcg cgcttccatg cagaccctga aagcggacat cactttttat ccggaggagg
     2821 ccttgtccgg actgaacgat gatttttcca gaaccgtctg ttatgattcc atggcccgtg
     2881 cgctccgggc ggaggccatg gcgcgcccgc gcaagctggt ggaaacgctg gcggaggata
     2941 tgggaaaggt ttgcctgaag gagttcggcg cgcggcacgt ggttgttact ctgcgcaagt
     3001 tcatcctgcc gcggacggac agcgtttccg taacggtgca tgtttcccgt caccggtagg
     3061 aaagaggttt tctaccggat atccaccgcc ttttcttcag ggatcttttt tccgtctttg
     3121 gtgaaagtgt ttttctccgc cttgacgctg ccggtatggc gagtgcggat ggcggttccg
     3181 ttcttgaggt caaatgtgtt tccggtaatg gtgatgttcc ggtgaacttc cccttccggc
     3241 cgtggggcgt gcggcacgat ctggatgacg gcttctccgc attgaataaa ggtattgcgg
     3301 gaaatggtca tattcctgac cacgccggat tcataccagc cggaggcgtc atcttccacc
     3361 aggatggcgg gcatgcccgt tttctcaaac cggcaccgtt caaccagcac cggcttcctg
     3421 gtggtaagca ggaagccccg ggtgggaatg tggcggcata ccgtgttgct gatatggacg
     3481 gatggcgtcc atgtgacgtt ttccacaaca tcatcaggct ggatgttttc gggattggga
     3541 tgttgcagcg taagttctat ttccttttca ttgagttggt tgacgccgga aacggtattg
     3601 gatgcataag gcaccagcgt gttgctggaa acataatcga tccggtctcc cggggcaaag
     3661 gcgtcaaagc cgaaggtctg gggatgcatg aaccggacgg tgattttgtt gggggccggc
     3721 ctgcctacaa tgcgcaagtg ggtgccatga acattgatgg cgtcgtcatg ggaggctccc
     3781 agcacgcaat ccttgacgat gatttttccc ctgcatccgg aaaagtgcag attatccgcc
     3841 caggaggagc tggtgcggag ggagcggggg cgcggagcta tgttcaagcg gctgaacatg
     3901 atgttctcgc agaactggga aacgacgccc atgccgtgca tatagtaaat atggcagtta
     3961 ttccagagga tgtttttgct tctgtattga aagaagccgc agcagtccct ccgggtattg
     4021 cggttctggt aggattggcc cggtttcatg gttggggagc cttccttgaa cgtgatgctg
     4081 atttttccgg gagacaactc tctgatgact gttttcccgg ggtcgaaacg gccctggaaa
     4141 gtgccggagg cggaatcaaa aactttggaa taccctccca tgcggaattc ccatccgggt
     4201 ccgtgccagg tcaggttccc atccttcacc gtgtacagga ttccgtccgg aatagttgct
     4261 ttcatggtat ccccttcaac ttccgtgacc agaaattccc ccatggtcgg atgcaggtaa
     4321 tcaaacccca ggccgttcag ggaaacgttt tccacatgtt ccatgcagac aaagatggct
     4381 tttccttcca gcaggatgtc ggtggctccc ggctttccct ccaggcgtat gtttttggct
     4441 tccatcaggc ggacagccat ggccttgggc tcgttgggcg catcgttggt gttggaaatc
     4501 tgcggttttc cggtcatcag ggcaccgtgt ttgaagatat gcctgccggg catgaaccgg
     4561 atggtgaccg gggcctgttt ggaaccttcc cccatcagtg ccagggagac ggcatgttcc
     4621 cccggatgga tgacgagcgt atctccccgc gccatgatga gacggttggc cggcgccatg
     4681 cttttccacg ccgtggatgg ggttgttccc ggattgccgt catttccctt gtccgggtct
     4741 atatgccaga catggtttcc tgcggattgc gtgcgctccg tccgttccgg ataatccgct
     4801 gccgggcatg gccctgtcaa cattgcgaga ataaaaagca gggagaccat ggggttttgc
     4861 atgctgtaac tcctacagga acacggtttt cgggacaaga ataattcttg cagggccggg
     4921 agaaaaagcc gcagcttttc catagctcac ctgggaggcc cctgtttttc atcctttctg
     4981 ccggggcggg aaagaaagcc taccagcttg tcataatacg ctttggattt gtcgtagtag
     5041 gattgggggt caatatccaa tttggtgcgg aacaggagcc agagcttgtg gaaggtgttg
     5101 acgcggtagc ggatgtcgaa aatacggaag ccgctcgcct tcattttgtc caaaatggta
     5161 tggtaaatga gcgccatggc ctgcgccgga ataagggcgg ctttgtcttc cgggctgagg
     5221 aggttatatt ccctctccgc ttccgcaaag aaagtttccg ccagatccgc ttcatatttc
     5281 agcagctcct gaagccgggg ggagtacacc ctgcagcgca ggtcttccag ggtgtatcca
     5341 aagcggtcca tatcctcctt gggcaaataa atgcggttgg acttgccgca gtcttccgcc
     5401 acgtcccgga tgatattcac catttgaagg gcatgcccca gggcaatggc gtacggatag
     5461 gaggcttctc ccgcccccat gatgcgggcg gaggtgaggc ccacgcagca ggctacgcag
     5521 taggtgtagt ccagcagttc atcgcggctg cggggctgct gctggcagat atccgaacgg
     5581 cagccttcaa tcaggtgcag catgggagtg gtgtccagct ccaaatcctg gatgagggct
     5641 atgatgtcct cctccagttc cgtcagttcc aggccttctc cgccgttgat gacttctacc
     5701 cagcggttca atgcgtcgtg gcgttcgcgg ggagtcatgc ccggttcgtc cacgatatcg
     5761 tccaccgtgc ggcaaaaagc gtaaaattcc gccatatggc ggcgttcttc ttcaggaaga
     5821 tcaatgagag caaaggccag gttggacttg gccttgctgg tgatggtttg ggcttgagtc
     5881 atgtcttgac ggaaaaaact tgcctgcgaa agggataaaa atcatggaat caggccccag
     5941 tgggaagtaa agggccacag gcagaagctg gcggggccta cgatgttgta ctggcggacg
     6001 gggccccagt accgggaatc aaaggaattt tcgcgggtgg aattgtcccc cagcgccacg
     6061 tattcacgca gataggggcg tttgctgtcg ttggacagat gcacggtccc cccttccgtg
     6121 atgtaagctc tgccgtccag caggctcagg ggggcgggaa gggccacgta gccgcagggc
     6181 tggtaggggg gcttgccgga agcgaccctc tggatagtcc gttccgtggc tggtttccca
     6241 ttgacgatta gctgggagtc ctgaatggag agggtatctc ccggaatgcc gcagaggcgt
     6301 ttgacgtagt gggttcctcc ttcctggccg gtgctgcttc ccttattggc gatgccttcg
     6361 atgccgcgtg tatcaaagac gaagacttcc ccgcgttccg gcttgcggaa gtgataggcc
     6421 attttattca ccacgattag gtccccggcg tcaaaacggg cattgacgat tgtgtctccg
     6481 ggcaggtagc ttctgaacgg ggtgtgccgt tcgtcttcaa agcgcggttc ctgattgagg
     6541 aagtagcggc gcgtttccgc ttccgctgcc ggaatttcca gcttggagcc gtcatcgaaa
     6601 atcacgttgg tcaccgtgag cagaagatac ttggtggcag gttcgaaacg gacgattttc
     6661 ttttccttgg aggccgtttc gtgcacatag gagccgccgt gcaggatcat gtccccgatt
     6721 ttctgcatca gggtgggctc cccttcctgg ggaagggcgc ggattccgtt cagggagggc
     6781 tgcatggaac cggtcgggat gcggaagggc tggactacat aacagcgcaa acccaggaaa
     6841 atggccagaa tcacgaacag aacttccacg ttttccgcca gggcggaagg aatcgctccg
     6901 gggatggatt ccagcgtgga ttccagtttg gcggtaatgg tttccgcctg ctgcctgtca
     6961 cctcggtaaa gggccgtttt cagcaggtcg cggctttctt caaactccgc caggcggtca
     7021 gtaggcagaa gatcgcgatt gtagttgatg tagcggcgca ctgctttcag ggccatacgt
     7081 cctttccggc gccatttggg cgtgaaccag gccagaggat gatccagaag aaacaccaga
     7141 acggcgtttg cagccggaaa gcaatcgtaa agggcagcta ggatggactt gtgcgggcta
     7201 ttcatgaagt acggcattca tgatgccccg tccggaggcg tcatggaagt ttattttacg
     7261 ttacggaagc cccatttttc caggattcag caaatttttt gggtccaggg cggatttgac
     7321 cagttcgtgc aggtggtagg cgccttcccc cacggctttc cggaaccatt tggccttcgc
     7381 caggccgatg ccgtgttccc cggtaatggc gcctccgtgt gccagcaccc agtggaacag
//
LOCUS       DAOMSU010000093         7411 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_773_length_7411_cov_2.507749, whole genome shotgun sequence.
ACCESSION   DAOMSU010000093 DAOMSU010000000
VERSION     DAOMSU010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7411
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_773_length_7411_cov_2.507749"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..1677)
                     /locus_tag="PK077_05375"
     CDS             complement(64..1677)
                     /locus_tag="PK077_05375"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN23765.1"
                     /translation="MDASFLHVLLAAAVAGCSSAAADGAHGYWMNSPSLNGWREAVFR
                     AGVPAQPAVLPPGVEPMMVGSSSPAAQMLVLEGMTHLLTFGDMRAFLKFDAALRLDPD
                     CLMAHWGRCMSLMGAGPAFQNQRVHSMKHMKELALRPDCPERERAYADALAVLLMDGP
                     VKAREAWKTICSTWKRDPYAPLFYAMLLRDGFDGQGNPGEGQKEAVRVVEAVLKERPG
                     SQAALFMRALLEEVAPSISPETVEIARRAVAANPFSASAHHLLGHCLFRTGDYEGASA
                     AFKESENLCLAWEKAENVPPALDDAYFRSILYRAVSEFCAGHYKKAEAIASRAASVPL
                     DKKHPLAPGTLLQLWEARTLPARLMLARPVIPRQALVLKAFPGPLPKGFPDLSNGMTA
                     VATQYMGACYAARQGRTDAVASAFDKMSGILRLLMDGADAARRQMSVSYWARCLQTGS
                     LYSSEIRSLMFPDSANVWMSEAIRSQRFSSLLLPPVMPYPAEWVLARAYLKAGKFREC
                     ADMCEQALKRFPNHAGVLETLDKARSSGK"
     gene            complement(1691..3487)
                     /gene="argS"
                     /locus_tag="PK077_05380"
     CDS             complement(1691..3487)
                     /gene="argS"
                     /locus_tag="PK077_05380"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRN23766.1"
                     /translation="MTTAVECPFIWADMTIPGILEEKLSSALKAVLGEELPADFRASV
                     TPSADLRFGDYQSNAAMVLAKRCRTNPRALAQQVADRIGQDAVCSLEIAGPGFINFRI
                     RPEFYAARLLAMLADDRLGVEKVQAPKTIVIDFSAPNVAKPMHVGHIRSTIIGDALSR
                     VARFVGHHVITDNHIGDWGTQFGMILWGWKNILDEQALAANPIDELLRVYKDVNLMCK
                     EKPELLDTCKAELVKLQAGDGENLAIWKRCVEVSKSGLSKIYDQLDIHFDYWLGESFY
                     NDALAPLVDGMIASGMARESDGAICVFSDGSVPPNEDPFLVQDKGEWRANPCIIRKAD
                     GGFLYATTDLATLDHRIRTWGADSIWYVVGAPQALHFRQIFSTQRRRGMDGDYRHIAF
                     GSILGDDRKPFKTRSGDTVSLQDVLDEAIERAARVVEEKSPDMPDEEKKRVAEVVGIG
                     AVKFAELSQNRMTDYVFNWDKMLALQGDTAPYLQNSYVRVRSIFRKLDGGPLDWSAPI
                     QLSEDAEIHLARLLARYGEVVPQVLDDCRPNVLAAYLFDLARAFHSFYEACPVLKSEG
                     PVRHSRLALCELTARTLKHGLGLLGIQLPDRM"
     gene            complement(3614..3751)
                     /locus_tag="PK077_05385"
     CDS             complement(3614..3751)
                     /locus_tag="PK077_05385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23767.1"
                     /translation="MKRKLASVLAKAFSGKSVPEKKREREARASKKLEAWLKASRMRS
                     F"
     gene            complement(4124..>4337)
                     /locus_tag="PK077_05390"
     CDS             complement(4124..>4337)
                     /locus_tag="PK077_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23768.1"
                     /translation="YRRTLINPYSLYMTVYRESFLMVVLFLCRHLFRLLPFIGIIGMP
                     VLCVTEYHLYRRMFLWKAEKYDFRGE"
     assembly_gap    4338..4347
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4348..4987)
                     /locus_tag="PK077_05395"
     CDS             complement(<4348..4987)
                     /locus_tag="PK077_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23769.1"
                     /translation="MKEPIPIQQWLPAGPLRDMGEKYVSQLPDVAQNPIGPESLMHQS
                     DHSWSEYLVAYSLLYPGVVVILALLGGLGLGAFFIFCRRREYSHRIFCSKCGSMMYPC
                     GLHCPECGTPNPSPRALNWIGYSRLRTVVPPSGWKRHEEVLRSYRRCFYCGQPLREPS
                     LDQRCPACGKAVLQGEQSVDRYDAYIGRRRGWTFAAVVVLGVVPILGPLLASS"
     gene            5173..5673
                     /locus_tag="PK077_05400"
     CDS             5173..5673
                     /locus_tag="PK077_05400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HRN23770.1"
                     /translation="MPTLRQAAKDDITLIHELACQAFPATYRDLLSREQIDFMMDWMY
                     SPANLEKQMEEGHVYFIASHEGKDCGYLSVQPEGPGVFHLQKIYVLPGFQGLHIGSFL
                     FRHAISYIRSIHPEPCLMRLNVNRDNTRAVEFYQRMGMRTLERGDFHIGHGYYMTDYI
                     MGLDIA"
     gene            5800..>6765
                     /locus_tag="PK077_05405"
     CDS             5800..>6765
                     /locus_tag="PK077_05405"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRN23771.1"
                     /translation="MTLNVEFFIIRSMNAFPAFILSGIIAAMAVPASAQAAESKVTYP
                     AFNDGSHIHGPKLKASDLKGKVVFFEYWGINCPPCIASMPHLQELQEKFQSKGFTVIG
                     SHSQLPSPRVKQFLEEKKITFPIYQSLSIPEAPCPGGLPHAVLIGANGKVVAKGYPPQ
                     LYDLVKKEVMKMERGLPILEGVELNKYKSLAKTVVSTGSNIESKITPLRKKTNDEEAQ
                     AVCEAFDAWLENTKEIVQARIQSDPLEAVTAIMRLKTAVPSVKEFDEPLAALKANRDL
                     SKLADLNKKISALEQRKAKGRKISESDLKSLTQAVDKFTESDNEAT"
     assembly_gap    6766..6776
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6883..7257)
                     /locus_tag="PK077_05410"
     CDS             complement(6883..7257)
                     /locus_tag="PK077_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HRN23772.1"
                     /translation="MREFVMASSPDVVEYICFQLRHAGDISFRKMFGDYGLYCGRTFF
                     GLVCDNQLYVKITESGLKMFPQQEQGCPYPGARPHFLVTDLDDDRTLSSLVRETCSIL
                     AVPSVLEGKKKKASGEKRSRHP"
BASE COUNT         1655 a         2295 c         2059 g         1381 t
ORIGIN      
        1 tccaccgctt ccaccgcttc caccgcttcc accgcttcca ccgcttccac cgcttattcc
       61 gaattatttt ccggaagaac gggccttgtc cagggtctcc agcactccgg catggttcgg
      121 gaagcgtttc agcgcctgtt cgcacatatc cgcgcattcc cggaatttcc cggctttcag
      181 gtaggcgcgg gccagaaccc attccgcggg gtatggcatt acgggaggca ggagcaggga
      241 ggaaaaacgc tggctccgta tggcctcgga catccagaca ttggcggaat ccggaaacat
      301 gagggagcgg atttcagaag aatacaggct gcccgtctgg aggcagcgcg cccagtagga
      361 aacgctcatc tgcctccggg cggcgtccgc gccgtccatc agcagccgca gaatgccgga
      421 catcttgtca aaggcggacg ccacggcatc cgttctgccc tgccttgcgg cgtaacatgc
      481 gcccatgtat tgggtggcca cggcggtcat gccgttgctc aaatccggaa atcccttggg
      541 aagaggcccc ggaaaagcct tgagcacaag ggcctgccgg ggaatgacag gccgggccag
      601 catcaggcgc gcaggcagcg tcctggcctc ccatagctga agcagggttc cgggagccag
      661 cggatgcttt ttgtccaagg gaacggaagc ggctctggag gcaatggcct ccgccttttt
      721 gtaatggccg gcgcagaatt cggaaacggc acggtaaagg atggagcgga agtaggcgtc
      781 atccagcgcg ggaggcacgt tttccgcctt ttcccaggcc aggcacagat tttcagactc
      841 cttgaaagcg gcggaagccc cttcataatc acctgtgcgg aacaggcaat ggcccagcaa
      901 atgatgagcg gacgcggaaa aggggttggc ggccacggcc ctgcgggcga tttccaccgt
      961 ttccggagaa atggatggag ccacttcctc cagcagggcg cgcatgaaca gggcggcctg
     1021 ggaaccgggg cgttccttca ggacggcttc caccacgcgg acggcctcct tctgcccttc
     1081 tccggggttg ccctgcccgt caaagccgtc ccggaggagc atggcgtaaa agaggggggc
     1141 gtatgggtcg cgcttccagg tggaacagat ggttttccac gcttcccgcg ccttcacggg
     1201 gccatccatc agcagcacgg ccagggcatc cgcataagcg cgttcccgtt ccgggcagtc
     1261 cgggcgcagc gccagctctt tcatgtgctt catggaatgg acgcgctggt tctggaaggc
     1321 gggccccgcg cccatcaggc tcatgcacct gccccagtgg gccatgaggc agtccgggtc
     1381 cagacggaga gcggcgtcaa atttaaggaa agccctcatg tccccgaacg tgagcagatg
     1441 cgtcattccc tccagcacca gcatttgcgc ggcgggagat gaggaaccca ccatcatcgg
     1501 ctccactccg ggcggcagca cggcaggctg cgccggaacg ccggcgcgga agacggcttc
     1561 ccgccagccg ttcagagacg ggctgttcat ccaatacccg tgtgccccgt ccgcggcggc
     1621 ggaagaacac cccgccacgg cggcggccag caggacatgg aggaaagagg cgtccatacg
     1681 gaagaaacgg ttacatcctg tccggaagct ggatgcccag caggcccagc ccgtgcttca
     1741 gcgtgcgtgc ggtcagctcg cacagggcca ggcgggagtg ccgcaccggc ccctccgact
     1801 tcaggaccgg gcacgcctca tagaaggaat ggaaagccct ggccaggtca aacaggtagg
     1861 cggccagcac gttcggacgg cagtcgtcca gcacctgcgg caccacttcc ccgtagcggg
     1921 ccagaagccg ggccagatgg atttccgcgt cttccgaaag ctgaatgggg gcggaccagt
     1981 ccaaaggccc gccgtccagc ttgcggaaga tggagcggac gcgcacgtag gagttctgga
     2041 ggtacggcgc cgtatctccc tgaagagcca gcatcttgtc ccagttgaaa acgtaatccg
     2101 tcatgcggtt ctgggacagc tccgcaaact tcacggcgcc tatgcccaca acttccgcca
     2161 cgcgcttctt ttcctcatcc ggcatgtccg ggctcttctc ctccaccacg cgggccgcgc
     2221 gttcaatggc ttcatccagc acatcctgga gggaaaccgt atccccggaa cgcgtcttaa
     2281 acggcttgcg gtcgtcaccc agaatggagc cgaacgcaat gtgccggtag tccccgtcca
     2341 tgccgcggcg gcgctgcgtg gagaaaatct gcctgaaatg cagggcctga ggggcgccca
     2401 ccacgtacca gatggaatcc gcgccccacg tcctgatgcg gtggtccagc gtcgcgaggt
     2461 ccgtggtggc gtacaggaag cctccgtccg ctttcctgat gatgcagggg ttggcgcgcc
     2521 attccccctt gtcctgcacg aggaaggggt cttcattagg cggcacggaa ccgtcggaaa
     2581 acacgcagat ggccccgtcg ctctcccggg ccattccgga ggcgatcatg ccgtccacca
     2641 gcggggccag ggcatcattg taaaagcttt cccccagcca gtaatcaaaa tggatgtcaa
     2701 gctggtcgta aattttggaa aggccggatt tggaaacttc cacgcagcgt ttccagatgg
     2761 ccaggttttc cccgtccccg gcctggagct tcaccagttc cgccttgcag gtatccagca
     2821 gttccggctt ttccttgcac atcaggttca catccttgta cacccgcagc agctcgtcaa
     2881 tagggttggc ggccagggcc tgctcatcca gaatattctt ccagccccac aggatcatgc
     2941 cgaactgggt accccagtcc ccgatgtggt tgtccgtaat cacatggtgc cccacaaaac
     3001 gggccacgcg ggaaagggcg tcaccaataa tggtggagcg gatgtggccc acatgcatcg
     3061 gcttggcgac gttgggagcg gaaaagtcaa tgacaatggt tttgggggcc tgcacttttt
     3121 ccacccccag ccggtcatcc gccagcatgg ccagcaggcg cgctgcgtaa aattccggcc
     3181 tgatgcggaa attgatgaat ccgggcccgg caatctccag ggagcacacg gcatcctgcc
     3241 ctatccggtc cgccacctgc tgggcgagcg cgcggggatt cgtgcggcac cgcttggcaa
     3301 gcaccatggc ggcgttgctc tggtaatccc cgaaccgcaa atccgcggag ggcgtcaccg
     3361 aggcgcgaaa atcagcggga agctcctccc ccaacaccgc ctttaatgcg gaggacaact
     3421 tttcttcaag aattccgggt atggtcatgt ctgcccagat gaacggacat tccaccgccg
     3481 tagtcaaggc gcaactgtca tcaagacata ccttttccgg taaaaacaac aggacggaga
     3541 taacatcccc gtcccagccg gcgaaccgaa cgcccggcgc ccgaatccac ccttcccttc
     3601 aacgggcgct tccttaaaag gagcgcattc tggaagcctt cagccacgct tccagttttt
     3661 tggaggccct ggcctccctt tcccgtttct tttccggcac ggactttccg gagaaagcct
     3721 tcgccagaac cgatgccagt tttcttttca ttttcatttt atttatatta cagtttttgt
     3781 tcctccgcat gcgggggttt gggaatcatc ccgccccccc tccggggaac actgcctaca
     3841 tactgtacca ggtaagttta gtccataaga aaaaaacaat tccttgtcat acttgataca
     3901 ataagacgta aaatgttcat taaaaacatc tctccatcaa ctccgcatcc cctgctgcgc
     3961 gtcaatcaag gagccggcca tcctttatcc ccatttcctg ccgccgcgcg ccataaagcg
     4021 ggctgccgga aacgatacgc tgcccggaag ccccaacagc cccgcacccc atgattttcc
     4081 gcgcaggcgc cggacaggca ttaccggaag agggtctcct gcttcattcc cccctgaaat
     4141 catatttctc cgccttccac aggaacatgc gccggtacag gtggtattcc gtcacgcaaa
     4201 ggacgggcat gccgatgatg ccgatgaacg gcagcaggcg aaacaaatga cggcacagaa
     4261 aaagaaccac catcaggaag ctttcccggt acacggtcat gtacagggaa tacgggttga
     4321 tgagcgttct cctgtacnnn nnnnnnnggg aggaagccag cagcggcccc aaaatgggaa
     4381 ccacccccag cacgaccacg gcagcgaagg tccaccccct gcggcggcca atatacgcat
     4441 cataccggtc cacggactgc tcaccctgga gcaccgcctt gccgcaggcg gggcaacgtt
     4501 ggtcaagaga gggctcacgg agaggctgcc cgcagtaaaa acaccgccgg tagctgcgga
     4561 gaacttcctc atgccttttc catccggagg gaggaaccac ggtgcgcagc ctggaatatc
     4621 ctatccagtt cagggcacgg ggggacggat tgggcgtgcc gcactccggg cagtgcagcc
     4681 cgcacgggta catcatggag ccgcatttgg aacagaaaat acggtgggaa tattcccggc
     4741 ggcggcaaaa aatgaagaaa gccccaagcc ccagcccccc cagcagggcc aggatgacga
     4801 ctacccccgg gtacaggagg gaataggcta ccaggtattc cgaccaggaa tggtccgact
     4861 ggtgcatgag ggattccgga ccgatgggat tttgcgccac gtccggaagc tggcttacgt
     4921 atttttcacc catgtccctc agaggccccg cgggaagcca ttgttgtatg ggaatgggtt
     4981 ctttcatgac tggacatgca gggttcgcca taaaaacagc atttcccgga aggctgtcag
     5041 aaaaacggcg gcattcagca taggatgtct ccgccggaat aggtagggga aaataggcca
     5101 tgccggaaaa ttccgccgga aatggggaag aggccctttt catccggcgc ttttccgtgt
     5161 aatatttctg ccatgccgac tctccgccaa gccgccaagg acgacatcac gctgatccat
     5221 gaactggcct gccaggcttt tccggccaca taccgggacc tcctttccag agagcaaata
     5281 gatttcatga tggactggat gtattccccc gccaacctgg aaaaacagat ggaggagggg
     5341 cacgtgtact tcatcgcctc ccatgaggga aaggactgcg gctacctgtc cgtccagccg
     5401 gaaggccccg gcgtcttcca tttgcagaaa atctacgttc tgcccggctt ccagggcctg
     5461 catataggaa gctttctgtt ccgccacgcc atcagctaca tcagaagcat ccacccggaa
     5521 ccgtgcctga tgcgcctgaa cgtgaaccgc gataacaccc gggcggtgga attctaccag
     5581 cgcatgggca tgcggaccct ggaacgcggg gatttccaca tcggccacgg ttactacatg
     5641 acggactaca tcatgggact ggatatagcc tgaaacgcct ctccctcagg gaaaaaacag
     5701 ccaaatcttc ccagggcagg gagtgccccg ccccccatcc ccggcgcatg cgggccgttg
     5761 aacccttagg aaactccagt tgaaaattcc gcttcctcct tgacactcaa tgttgaattc
     5821 ttcatcatcc gttccatgaa cgcctttcct gcctttattt tatccggtat catcgccgcc
     5881 atggcggttc ccgcctccgc ccaggcggcg gaaagcaaag tcacctatcc agccttcaac
     5941 gacggcagcc atatccacgg gccgaaactc aaagcttccg acctgaaagg gaaagtggtg
     6001 ttttttgaat actggggcat caactgcccg ccctgcatcg ccagcatgcc gcatctgcag
     6061 gaattgcagg aaaaattcca gtccaagggc tttactgtca taggcagcca cagccagctc
     6121 ccgtccccca gggtcaaaca gtttctggaa gaaaagaaga tcactttccc catctatcag
     6181 agtctgagca ttccggaggc tccctgcccg ggcggattgc ctcatgccgt tctgattgga
     6241 gccaacggaa aagtcgtggc caagggctat cctccccagc tctatgacct ggtgaaaaag
     6301 gaagtgatga agatggaacg cggccttcct attctggaag gagtggaact gaacaaatac
     6361 aaatcgctgg ccaaaacggt cgtttccacc ggcagcaaca tcgaatccaa aatcacacct
     6421 ctgaggaaaa aaacgaatga cgaggaagcg caggccgtat gtgaagcttt tgacgcatgg
     6481 ttggaaaata ccaaggaaat cgtgcaggcc cggatccagt ccgaccctct ggaagcggta
     6541 acggccatca tgcgcctcaa aacggcggtt ccctccgtca aggaatttga cgaacctctg
     6601 gcggccctga aagcgaacag ggatttatca aaactggccg acctcaataa aaaaatctcc
     6661 gctctggaac agcgcaaggc aaaagggcgc aaaatatcgg aatccgacct taaatccctg
     6721 acgcaggccg tggacaaatt cacggagtcc gacaacgaag ccacgnnnnn nnnnnncaaa
     6781 ccgccgcctc cagcctgaag aagaacctct cctctctggc cgcttcggaa actcccggga
     6841 aataaccttc ccctccagca gccatttcct gccggcaggc gtctaagggt gcctgcttct
     6901 tttttcaccg gacgcctttt tctttttccc ctccagaaca gacggaacag ccagaatgga
     6961 gcatgtctcc ctgacaaggg aggaaagggt ccggtcatcg tccagatccg tcaccaggaa
     7021 atgcggcctg gcgccgggat aggggcagcc ctgctcctgt tgcggaaaca tcttcaaacc
     7081 cgactccgtt atcttgacat acaactggtt gtcacacacc aaaccgaaga atgtccggcc
     7141 gcagtacaat ccgtagtccc caaacatttt tctgaaggaa atatccccgg catgccggag
     7201 ctggaaacaa atatattcta ctacatccgg ggaagaggcc atgacgaact ctctcatgcc
     7261 tcccccgtcc gctgcaagaa gcaaatgacg gccgggaagg cttccggcag gaatgaggca
     7321 tccccgttat tgacgggaaa aggcgcagca tgccccacgg agtcatgacg aacggcatcc
     7381 atgccgccgc agccgctccg caagaaaatc c
//
LOCUS       DAOMSU010000094         7321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_791_length_7321_cov_3.105010, whole genome shotgun sequence.
ACCESSION   DAOMSU010000094 DAOMSU010000000
VERSION     DAOMSU010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7321
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_791_length_7321_cov_3.105010"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4260)
                     /locus_tag="PK077_05415"
     CDS             complement(<1..4260)
                     /locus_tag="PK077_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRN23773.1"
                     /translation="MPVPFNKEAGAFSGSWDWGIVVDPLGPGETAECRLMLGVDDNGS
                     LTVDGEGIFIPGEGKYHGGSYREKSLSFPIEPGPHRVHMTYENVAVPPEWTNLAILNY
                     SIEVMVSDGTSSSSYQPDEGYPEPVSTEDEGEDVPCECGEEAAGGNGSQSSSSSPCPE
                     EDNGEGDNGEDEDPCTCENNEGGSSSSARGARSSSTLYGSSAAGRRVVARTLKTEMIW
                     RTNFGSFRGMTGLPQGLLEIVGYTFSAELWSPRALHYWHPMTREIISAPSSGIGANTA
                     FQIRSGGTAINYYCYADGDGSRGDSVAPIAGSAKRGGSAWFGRRSASPARAGEFCLSI
                     RSNAGNTVNYTAGSASSLKYATGYTAKNGAAYTREEFDGKLDIVRASDGSIRQIWNLW
                     DGLANIENVTESGYRIALYLPEQVGGKNSSTGLYSVTGDPFKTFTITGDAAAGRLSVT
                     EQTAGRAPYTTRYWQGADGAWNMAQGEGEDAIFTLKEKQIVSPGTWKLITTIQRGENG
                     APISRVCETYAVTRNGNLCTSRIEGYGTDYARETTYEYTGMGKIAKETAPDGSVKTWA
                     YDRFGREIVSSVPWAEAGDKVTYTTYRDQTQADPDILTQWVTLTATAAELWRADYTYV
                     EENHVRRVEKRTTALGEENVRLEVKESWLGTAPNVYARGRLKMKQGMDGIQTHYFYEE
                     TDQYGALYKVTAETRIAGESVPGLSSRKVTYVSGQGNNMRYEQYARLADGAWSMTDAS
                     SYEYDVENRWVKRTRANGRVYERMMTCCGPLWERDENGVTTSYSYNTARQLVETIRSA
                     VADGETMITPETITSYTRDALGKITSLRRDAGPMTTVESREYDLLGRLIRETDILGRT
                     TVRSYSGDGLVETVTTPAGATLITRKSPSGAVVRRYGTGQQDILYTVEATAEGIRTTE
                     AVPGGEGGAPRVVTGSSTVNGFGELVRIAAANTLNGENVRTLAYDDKGRLIREQLADM
                     APTLYAYDAFGNRTRAIVALEDEPTFLNSRITDYAYVAESREDGIYRLVSITSYTSSG
                     TPVLQKRAALLSALSSVLAGKTVMTDPRGQDTVEWTEYGGGSVRLQKKTVPGVGSVAL
                     TRVVDGFTTAVTGFDGATARRQRTYTETGITYADTDVRGNVFTAVCDIAGRIISGTDA
                     AGNTTTYAYAQPFDLPTCVTNALGKTACSVYDIRGRKEAEWGTAIQPAVYAYDAAGHI
                     VSLSTFRDPGDVITTDPRLRTDGDITTWTYDVATGLVVRKTYANATHVDTAYDALNRV
                     SATTDARGTVASRSYAPLTGELTSITFNDDGFTPSISSRYNHLGQLTQIEDASGTRTF
                     TYNQYNEQETETTAGLAASVLTLRRDGVGRPAGYCLDYAGAAALQTAWGHDASGRLSS
                     VSLNAVAKPFTYGYNAENGLLDTLDYPNTLKRWRTLEEKRDL"
     gene            4745..6313
                     /locus_tag="PK077_05420"
     CDS             4745..6313
                     /locus_tag="PK077_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HRN23774.1"
                     /translation="MNNTTQPGAAPTGPATTNNAPAVKSVLRMGVFTLAMINVSAIVS
                     LRGMSAESTYGLSSVFYYIFAAVFFLVPVSLVAAELTTGWPQKGGVYRWVGEAFGKKW
                     GFLAIWLQWIESTIWFPTVLTFAAVSLAFMGPGQRWDEALAANKWYVLIVVLCVYWAA
                     TLLNLRGMKTSAGVTKWGTIIGTIIPGAILILLGLGYWAGGNPILLDMSWDKLVPDMS
                     NFNNLVLAASIFLFYAGMEMSAVHVKDVNNPGRNYPLAILISAIITVLIFVLGTLAIG
                     FIIPNSQINLVQSLLITYDSYFSFFGLGWMNWILALALAVGVLAQVTAWVGGPSKGLY
                     QVGLAGNLPPVMQKRNKNNVQMGILFIQGGIVTLLSIMFVIMPSVQSAYQIISQLTII
                     LYLIMYMLMFASGIYLRYREPNTPRTFRIPGGRTFGMWIVGGLGFLASLAAFLVSFIP
                     PNQITVGSSTMYILLLVVGTFIFAGIPFIIHAMAKPSWKRPVDPEDAFEPFGWEKNND
                     SHSAATPSHSISHE"
     gene            6306..7247
                     /gene="glsA"
                     /locus_tag="PK077_05425"
     CDS             6306..7247
                     /gene="glsA"
                     /locus_tag="PK077_05425"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HRN23775.1"
                     /translation="MSNIPSNEQIRTALDNAYAYAKTVQGGKNASYIPALAQVPSDLL
                     AIAVVTVNGDLLTAGSADAPFAIESISKAFNLAYVMDLIGMKQLRAKIGADPTGEPFN
                     SVMAIELHGGKPLNPLVNAGAMATVSLVNGSDSDEIWGNMIHNFNNFANTTLTVNQEI
                     YKSESATNQHNRGIAWLLDSYGYFYNTPPMIVDLYTRMCSLNITASQLALMGACYANG
                     GVNPVSKKRVVKEENVPPILAEMCMSGLYDSTGDWMYKAGLPAKSGVGGGLVAVAPGK
                     LAMAAFSPPLDPAGNTVKGQAALQSIIRELNLNLFRS"
BASE COUNT         1492 a         2171 c         2007 g         1651 t
ORIGIN      
        1 gagatcgcgc ttttcttcca aagtgcgcca ccgtttcagg gtgttggggt aatcaagagt
       61 gtccagcagg ccgttttccg cattgtagcc gtaggtgaag ggcttcgcga cggcgttcag
      121 cgagacggag gaaagccttc cggaggcgtc atgcccccat gccgtctgca aggcggcggc
      181 gcccgcatag tccagacagt agcccgcggg ccgccccact ccgtcacggc ggagggtcaa
      241 gacgcttgcc gcaaggccag ccgtcgtttc cgtttcctgt tcgttatact gattgtacgt
      301 gaacgtgcgc gttccggaag cgtcttcaat ttgcgtcagc tgccccagat gattgtagcg
      361 gctgctgatg gagggggtaa aaccgtcgtc attaaaggta atggaggtaa gctccccggt
      421 aaggggggcg taggagcggg aggcaacggt tccccgcgcg tccgtcgtag cggaaacgcg
      481 gttgagcgca tcataagcgg tatccacatg ggtggcattg gcataggtct tgcggacaac
      541 cagccccgta gcgacatcgt acgtccaggt ggtgatgtcc ccatccgtcc ggaggcgggg
      601 atcagtagtg atgacatccc cgggatcacg gaaggtggaa aggctcacta tatggccggc
      661 ggcgtcataa gcgtatacgg cgggctgtat ggccgtcccc cattcggctt ccttgcggcc
      721 ccggatatca taaacggaac aggctgtttt accaagggca ttggtaacgc aggtaggcag
      781 atcaaagggc tgggcataag cgtaggtggt cgtgttgccc gcggcatccg tgccggaaat
      841 gatgcggccg gcgatgtcgc agacggcggt aaagacgtta ccgcgcacat ctgtgtccgc
      901 ataggtgatg cccgtttccg tataagtgcg ctgccggcgg gcggtcgcgc cgtcaaagcc
      961 cgtaaccgcc gtcgtgaatc cgtccacgac gcgcgtgagg gcaacgctcc cgacgccggg
     1021 cacggttttc ttctgcaggc ggacagaacc gccgccatac tccgtccact ccacggtatc
     1081 ctggccgcgg ggatccgtca tgacggtttt gccggcaaga acagaactca gagcagagag
     1141 cagggccgcc cgcttttgaa gaacgggagt acctgaagaa gtataacttg ttatggaaac
     1201 aagtctgtag atgccgtctt cacggctctc ggccacataa gcgtagtcag taatgcggga
     1261 gttcaggaag gtcggttcat cctccagggc gacgattgcc cttgtcctgt tcccaaaggc
     1321 atcataggca tagagtgtcg gcgccatgtc cgccagctgt tccctgataa ggcgcccctt
     1381 gtcatcgtat gcaagagtgc gcacgttttc cccattcagg gtattggcgg cagcaatccg
     1441 tacaagctcg ccaaagccgt tgacggtgga gcttcccgtc acgacgcggg gcgctccccc
     1501 ctccccgccc ggcacagcct ccgtcgtgcg gatcccttcc gccgtggcct caacggtata
     1561 aaggatatcc tgctgcccgg tgccgtaacg ccgcactact gctccggaag ggcttttccg
     1621 ggtgatgagc gtagcgccgg cgggcgtggt aacagtttct accaggccat ccccgctata
     1681 agagcggacg gtggtgcgcc ccagaatatc ggtttccctt atcaggcgcc ccagcaaatc
     1741 gtattcccgg ctttccaccg tcgtcatggg gcctgcatcc ctgcgcaggg aggtaatttt
     1801 accgagggca tcacgggtat agctcgtgat ggtttccgga gtgatcatgg tttccccgtc
     1861 cgcaacggct gaacggatgg tttccaccag ctggcgggcc gtattgtagg aataggaagt
     1921 ggtcactcca ttctcatccc tctcccacaa cgggccgcag caggtcatca tgcgctcgta
     1981 aacgcgccca ttggcgcggg tgcgctttac ccaacggttc tcaacgtcat attcataaga
     2041 agacgcgtcc gtcatggacc aggcgccatc ggccagccgg gcgtattgct cataacgcat
     2101 gttattgccc tggccggaga cgtaagtaac cttccgcgag ctcaggccgg ggacgctctc
     2161 tcccgctatc cgcgtttccg cggtcacttt gtaaagggct ccgtactggt ccgtctcttc
     2221 ataaaaataa tgcgtctgaa tgccgtccat gccctgtttc atcttcaggc gtccgcgggc
     2281 ataaacattg ggagccgtgc cgagccagga ttccttcact tccagacgca cgttttcttc
     2341 ccccaatgcg gtcgtgcgtt tttccacgcg ccgcacatga ttttcctcaa cgtaggtgta
     2401 atccgcgcgc caaagttccg ccgcagtggc cgtcaacgtc acccattggg tgaggatatc
     2461 cggatccgcc tgcgtctggt cccgatacgt ggtgtaagtg accttatcgc cggcttccgc
     2521 ccaggggacg ctggacacga tttcacggcc gaaacggtcg tatgcccacg ttttgacact
     2581 tccgtcagga gcagtttcct tcgcgatttt tcccatgccg gtgtactcgt aagtcgtttc
     2641 ccgggcgtag tccgttccgt agccttcaat acggctggtg cacaaattgc cattgcgggt
     2701 gacggcataa gtctcacaga cgcgggagat gggagcgccg ttttcccccc gctggatggt
     2761 ggtgatgagt ttccacgtgc cgggcgaaac gatctgtttt tctttcagag tgaatatggc
     2821 gtcttctcct tccccctgcg ccatgttcca tgctccgtcg gctccctgcc agtagcgggt
     2881 ggtgtagggc gcgcgcccgg cggtctgttc ggtaacggag agtctgcccg cagccgcgtc
     2941 cccggtaatt gtgaaggttt taaaggggtc gcccgtgacg gaataaaggc cggtggaact
     3001 gtttttcccg cccacctgct cggggagata gagggcgatg cggtaaccgg actccgtgac
     3061 attttcgatg ttggcgagcc cgtcccacag gttccatatc tgccggatgc ttccatccga
     3121 agcccgcacg atatccagct ttccgtcaaa ttcttctctt gtgtaagccg cgccgttttt
     3181 agcggtgtag ccggtggcgt atttcaagga agaggccgaa cctgcggtat agttaacggt
     3241 attgcccgcg tttgaacgga tactgaggca gaattctccc gcccttgccg gagaagcgga
     3301 tctcctcccg aaccacgccg agcccccgcg cttggcggat cctgcaatgg gggccacgct
     3361 gtcacccctg ctgccgtctc catccgcgta acagtagtag ttaatggcag ttcctccgct
     3421 cctgatttgg aaagcggtat tcgccccgat gcccgatgat ggcgcggaga tgatttcacg
     3481 agtcatcgga tgccagtaat gaagcgcacg tggggaccag agctcagccg agaatgtgta
     3541 tcctactatt tccagcaatc cctgaggcag acccgtcatg ccccggaaag agccgaaatt
     3601 ggtacgccag atcatttccg tcttgagcgt ccgggcaacc acgcgcctgc cggcggccga
     3661 cgaaccgtaa agggtggagg aagagcgcgc gccgcgcgcc gaagagctgc tgccgccctc
     3721 attgttttca caggtgcagg gatcctcatc ttcaccgtta tccccttctc cgttgtcttc
     3781 ctccggacag ggagaactgg aactgctctg ggagccattg ccgccagcag cctcctcccc
     3841 gcattcacac ggcacgtctt ccccctcatc ttccgtactt acgggttccg gataaccttc
     3901 atccggctga taggaggatg aggaggttcc atccgaaacc atgacttcaa tggaataatt
     3961 aaggatggcg agattagtcc attcaggagg aacagccacg ttctcataag tcatgtgcac
     4021 gcggtgcggt cccggttcaa tggggaagct caagcttttt tccctataag accctccatg
     4081 gtatttgccc tccccaggaa tgaaaattcc ctcgccgtca acagtaagag agccgttatc
     4141 atccaccccc agcatcagac ggcactccgc cgtttccccc gggccgagcg gatcaacgac
     4201 aatcccccag tcccagctgc cgctgaacgc cccggcctct ttgttaaacg ggaccggcac
     4261 acctccggac gctgtgaggg aattatcagg cagagaattc gcatgacgct gaacctgttg
     4321 atctttcata aaatccagat aattgacgat tgagggacga acatcaaaga agacaacgct
     4381 tctctatatt cactattctt atgtatctcg gaaaacggaa cagatcaatg attcattttt
     4441 gaataattgt tactaattac aataaattat taccaatcaa tattctttta aaatatatat
     4501 ttattcccaa caaccggagg gaataaccat gtttttccgc gctgcctgac atcccccgca
     4561 tctactgccg gccaaatggc catggggacg ggctgaacaa cttccccggc ctccgttaag
     4621 acaaactccg gcagatctat ctgcatcatc ccttcaacga aaggttctcc cgtctggtcg
     4681 tccagataag gccggtgtgg gcactttctt tacgccgtcc caattctcaa cccggaataa
     4741 accaatgaat aatacgactc aaccaggggc tgctccaaca ggccccgcaa caaccaataa
     4801 cgctccggcg gtaaaatccg tcctccgcat gggagtattc actctcgcca tgatcaacgt
     4861 ttccgccatt gtcagccttc gcggcatgtc tgcggaaagc acttacggac tgagttccgt
     4921 tttttattac attttcgccg cagtattctt tctggtacct gtttccctgg tggccgcgga
     4981 gcttactacc ggatggcccc aaaagggcgg cgtttaccgc tgggtaggcg aggctttcgg
     5041 gaaaaaatgg gggttcctgg ccatctggct gcaatggatt gagagtacca tctggttccc
     5101 taccgttctg acattcgccg ccgtttccct ggctttcatg gggcccgggc aaagatggga
     5161 tgaagcgctt gccgccaata aatggtatgt tctcatcgtg gtgctgtgcg tgtactgggc
     5221 ggccaccctg cttaatctgc gcggcatgaa gacttccgca ggcgtcacca aatggggaac
     5281 catcatcgga accattattc ccggagccat cctgatcctg ctgggcctgg gctattgggc
     5341 cggcggcaac ccgatcctac tggatatgag ctgggacaag ctggtgccgg acatgagcaa
     5401 tttcaacaac ctcgttctgg cagccagcat cttcctgttt tacgcgggga tggaaatgtc
     5461 cgccgtgcat gtgaaggatg tgaataatcc cggacgcaat tatccgctgg ccattctgat
     5521 ttccgccatc attacggtgc ttattttcgt tctgggcacg ctggccatcg gcttcatcat
     5581 tcccaattcc cagattaatc tggtgcagag cctgctgatt acttatgaca gctatttcag
     5641 cttcttcggc ctcggctgga tgaactggat tctggcgctt gcgctggccg tcggcgttct
     5701 ggcccaggta accgcatggg tgggaggccc ctcaaaaggc ctgtaccagg tgggcctggc
     5761 cggtaacctt ccgcctgtca tgcagaagcg gaacaagaac aacgtccaga tgggcatcct
     5821 ttttattcag gggggaatcg tcaccctgct ttccatcatg tttgtgatca tgccttccgt
     5881 gcaatccgcc taccagatta tttcccagct gaccatcatt ctgtacctca tcatgtacat
     5941 gctgatgttc gcgtcaggca tttacctgcg ctaccgggaa ccgaatacgc cccgcacctt
     6001 ccgcattccc ggcggcagaa ccttcggcat gtggattgtc ggagggctcg gcttcctggc
     6061 cagcctggcg gctttcctgg tgagcttcat cccgcctaat caaattaccg taggcagcag
     6121 taccatgtac attctccttc tggtggtggg cactttcatt ttcgcgggta tccccttcat
     6181 catccatgcc atggccaaac cctcctggaa acggccggtg gatcccgaag acgcctttga
     6241 acccttcgga tgggagaaaa acaatgattc ccattccgca gcaaccccta gccattccat
     6301 atcccatgag taacattcct tccaatgaac aaatccggac ggccctggat aatgcgtacg
     6361 cctacgccaa aaccgtccag ggaggcaaaa acgcctcgta cattcccgcc ctggcccagg
     6421 tgccttccga cctgctggcg attgccgtgg tcaccgtcaa cggagacctc ctgaccgcag
     6481 gctccgcgga cgcgcccttt gccattgaat ccatttccaa ggcattcaac ctggcttatg
     6541 tcatggatct gatcggcatg aagcagctgc gcgcgaaaat cggagcggac cccaccgggg
     6601 aacccttcaa ttccgtaatg gccattgaac tgcatggcgg caagcccctc aatcccctgg
     6661 tcaacgcagg ggccatggcg acggtcagcc tggtcaacgg ttcggattcc gatgaaatat
     6721 ggggaaacat gatccataat ttcaataact tcgccaacac gaccctgact gtgaaccagg
     6781 agatttacaa gtcggaaagc gctaccaacc agcacaaccg cggcatcgcc tggctgctgg
     6841 acagctacgg ctatttttac aatacgccgc ccatgattgt ggatttgtac acccgcatgt
     6901 gttccctgaa catcaccgcg tcccagctgg ccctgatggg cgcctgctac gccaacggcg
     6961 gggtcaatcc cgtcagcaag aagcgtgtgg tgaaagagga gaacgttccc cccattttgg
     7021 cggaaatgtg catgtcaggc ctttatgatt ccacagggga ctggatgtac aaggccggcc
     7081 ttccggccaa gtccggggta ggaggcggcc tggtggccgt agctcccggc aagctggcca
     7141 tggcggcttt ttcgcctccc ctggatcccg cagggaatac cgtcaagggg caggcggcgc
     7201 ttcaatccat catcagggaa ctgaatctaa accttttccg gagctgatcc gggatgagaa
     7261 ggtaaacccg cggagccaaa atccgaaagg attcccgcgg gtttactgta tcctgtccag
     7321 c
//
LOCUS       DAOMSU010000095         7276 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_796_length_7276_cov_2.759590, whole genome shotgun sequence.
ACCESSION   DAOMSU010000095 DAOMSU010000000
VERSION     DAOMSU010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7276
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_796_length_7276_cov_2.759590"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PK077_05430"
     CDS             <1..594
                     /locus_tag="PK077_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRN23776.1"
                     /translation="KAVGERIPVTAKIRIGWDFQSLCAMDACRLLENEGISMITIHGR
                     TRSQQYSGTANWDIIDECARAMSVPVIGNGDISTPQAVAYVKENTAVSGVMIGRAAME
                     NPWIFGDAKYYLLHGAMPPARDPQEKAALILRHARMALESRHYGDELHTMRHMRSRIL
                     AYTKGFPGAKEMRSRLVRVSSMPELEDLLSKLPRQKP"
     gene            806..1108
                     /locus_tag="PK077_05435"
     CDS             806..1108
                     /locus_tag="PK077_05435"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23777.1"
                     /translation="MYSNESYLIELLTQAGYLNEEILQYARSQKSPTQDLVDFLIQAN
                     YLTEDVIAQVAASNSLLPVVDLGSMHIPQEVRELITPELARRFRAIPISDDGFSIN"
     gene            1131..2438
                     /locus_tag="PK077_05440"
     CDS             1131..2438
                     /locus_tag="PK077_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRN23778.1"
                     /translation="MDSLPQLMGRDVIFSVATRSAVESRLNEFYRDLSVPEETDGLEG
                     EDAPIIRLVQQMLTDAFKMRASDIHIEPMENRLRIRYRVDGKLVEVATHPKKLLSPII
                     ARLKVMSTTMSIAEKRMPQDGRIQMSIGGKQIDLRVSSVPSNHGESIVMRILDKSALV
                     LGLPQLGFFSDDEAVFDRLITLPDGIILVTGPTGSGKTTTLYACLNHINRPDKKIITV
                     EDPVEYELSGINQVMVKADIGMTFAAALRAMLRQAPNIIMIGEIRDMETASIAINASL
                     TGHLVFSTLHTNDAPSAVARLADIGIKRFLIASSVRAIMAQRLVRKLCDRCKVDGTLT
                     EKQAHTLNIDMSRLAQGQIKAPHGCDFCRGGGFKGRMGLFEIFEIDDEVRRMINENLT
                     SPQLRQRARELGMRTLREDGVRKVLAGLTSPEEVLNVTMGDAN"
     gene            2467..4125
                     /locus_tag="PK077_05445"
     CDS             2467..4125
                     /locus_tag="PK077_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRN23779.1"
                     /translation="MDTNLTLELFIGRGMIDKSLAKDIKEEMIASGKELPEVLADFGI
                     IGSKDDIWQMIASDLGTEFITLDNFQPDPNVQNMMPATLVRLHGALPVRHGPEGLYVC
                     LVDPLNPQTVEDLRFALGQDIHVLVAPDYQISERINELYGGESAAMSDLMQELNNMQV
                     NNETEDSAAAPVIRFVDLVITQAIKEKASDIHFEPFEKEFKIRYRVDGALYEMQPPPV
                     HLSVPVISRVKVMANMNIAERRIPQDGRIVKQIGNRSVDMRVSSLPTQYGESVVLRVL
                     DRSSVNLNMDNLGLPAHIHEYILDTVHKPNGIFIVTGPTGAGKTTTLYAALREINTID
                     SKVLTAEDPVEYDIDGIIQIPINEAIGLDFPMVLRAFLRQDPDRILVGEMRDMATAQI
                     AIQASLTGHLVLSTLHTNDSAGAITRLVDMGCEPFLVAASLEGVLAQRLVRTICPDCR
                     TPYEPSSTILSQLGVSPYELGDKHFFTGRGCDKCSSSGYRGRKGIYELLDINDTLRDM
                     ITDRAPSVVLKQKAIEMGMSTLREDGLRNIYDGNTTIEEVLKYT"
     gene            4197..5465
                     /locus_tag="PK077_05450"
     CDS             4197..5465
                     /locus_tag="PK077_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRN23780.1"
                     /translation="MPKYQYTALDHKGDQKTGSLEANSEAEAMEAIRAHGLYPTQIVE
                     AGKGKIQQTPAAKKKAKGAKKQKGKLGGKIKAKALMIFTRQLATLIDAGLPLLQSLNV
                     LAKQEANPNLRVTIEALGDSVQGGSTFSEALAQHPRIFDRLFVNMVKAGELGGVLEVV
                     LNRLAEYQEKAQKLKSKVISAMVYPSIVLFIAVGIVIFLMLVIVPKFKAMFAEQNQEL
                     PGISEFVFGISDWFMAAPFFVPNAVILAAVVAILYAVFTAMSKTPNGRRKIDSALLTM
                     PVIGNVQSKSAIARFARTFGTLVTSGVPILQALTITKDTAGNMIVGDAIGLIHDSVKE
                     GESVVTPMSSSKLFPPMVISMVDVGEETGQLPDMLLKIADVYDDEVDNAVGAMTSMLE
                     PIMIVFLAVVVGGIVFAMFLPLLQVIEKMG"
     gene            5484..6152
                     /locus_tag="PK077_05455"
     CDS             5484..6152
                     /locus_tag="PK077_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRN23781.1"
                     /translation="MKNHSAYRRLRETKGFTLMEILVVMVIIVVLATLTISIYTWLET
                     RKNEQATESIVRKIEMGLESYHSDHDRYPYGTEAPFNNHGVAVANGEEYSSNVVYMAL
                     FGDHKNEGVPSRDTTIYNDELNPSTQPKSNPTVREVHVKSKDGRPVTLYILADPWGSP
                     YRYRLGSEQSIPTRTDRAKNLKMGNGLNPDYDFWSFGKDGDSDLKDPHASENEDDIGN
                     LPKF"
     gene            6287..7231
                     /locus_tag="PK077_05460"
     CDS             6287..7231
                     /locus_tag="PK077_05460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRN23782.1"
                     /translation="MKRISLIVPCLNEEEAIPAFKQEIDRVFKECLEGYAPELIFVDD
                     GSTDRSLEMIKKMAEHSPEVKFISFSRNFGKEAAIYAGLEAATGDYAAVMDVDLQDPP
                     SLLPDMLKAIEKEGYDCAGTRRVTRQGEPLLRSFFARAFYKTIHQISDTRIVDGARDY
                     KLMTRPVLEALLSLKEYNRFSKGLYEWVGFRTKWFEYENVERVAGETKWSFFKLFLYS
                     IEGIVAFSTAPLALASIMGVLLSFISFLSIIILTVRELIWPHSAYGWTSLVCVFCMMG
                     GIVLLCLGILGQYLAKTYTEVKKRPIYIARERSIKPFN"
BASE COUNT         1699 a         2136 c         1919 g         1522 t
ORIGIN      
        1 aaagccgtag gagagagaat ccccgtcacg gccaaaatac gtattggctg ggattttcag
       61 agcctgtgcg ccatggacgc gtgccgcctg ctggagaatg agggaatttc catgatcacc
      121 attcacgggc gcacccgctc ccagcagtat tccggaacgg ccaactggga cattattgac
      181 gaatgcgcgc gggccatgtc cgtgccggtc attgggaacg gggatatttc caccccgcag
      241 gctgtagctt atgtaaaaga aaatacggcc gtcagcggcg tcatgattgg ccgtgcggcc
      301 atggaaaacc catggatttt cggagacgcc aagtattatc tgctgcacgg agccatgccg
      361 ccggccagag acccgcagga aaaggcggcc ctgattttgc ggcatgcccg gatggccctg
      421 gagtcccgcc attatgggga tgaacttcac acgatgcgcc atatgaggtc acgaatcctg
      481 gcttatacga agggatttcc cggcgccaag gaaatgcgtt cccgcctggt gcgcgtatcc
      541 agcatgccgg agctggaaga cctgctttcc aagctccccc gccaaaagcc ctgagaacag
      601 gaaaaatccc gctttccggg agatttcgca ggtttggaaa gcggcggaaa gaggaggagg
      661 ttcttcccgc ctccggacac gcctgaaaaa aaatgccatt ttgagggaag ctaagttgac
      721 tttacgttcc gaacctgtca gaaatagggg agtatcctgc cgctccattt cggcagactt
      781 ccaacaatag catccagaga tcgacgtgta ctccaacgaa tcatatctca tcgaacttct
      841 tacgcaggcc ggatatctga atgaagaaat tcttcagtac gcgcgcagtc aaaaatctcc
      901 cacccaggac ctggtcgatt ttctgattca ggccaattat cttacggaag acgtcatcgc
      961 tcaggtggcg gcgtcaaact ccctgctccc cgtcgtggac ctgggctcca tgcacatccc
     1021 ccaggaggtg cgcgaactca ttacaccgga acttgccagg cgcttccggg ccatccccat
     1081 ttccgacgac ggtttttcca tcaattgacg accccctgaa tctggaaacc atggacagcc
     1141 tgccccagct gatggggcgg gacgtgattt tcagcgtcgc cacccgcagc gcggtggaaa
     1201 gccggttgaa tgaattttac cgggatctga gcgttccgga agaaaccgac gggctggaag
     1261 gggaggatgc tcccattatc cgtctggtgc agcagatgct gacggacgct ttcaaaatga
     1321 gggcttccga catccacatt gagcccatgg aaaacaggct ccgcatccgc taccgcgtgg
     1381 acggcaagct ggtggaggtg gccacccatc caaaaaaact gctcagcccc atcatcgctc
     1441 gcctcaaggt aatgagtacc accatgagca ttgcggaaaa acgcatgccc caggacgggc
     1501 gaatccagat gagcatcggc ggcaagcaaa tcgacctccg tgtttcatcc gtccccagca
     1561 accacgggga aagcatcgtc atgcgtatcc tggacaaatc cgccctggtg ctgggccttc
     1621 cccagctcgg atttttctca gatgatgaag ctgtgttcga ccgcctcatc acgctgccgg
     1681 acggcattat cctggtgacg ggtcctaccg gttccggtaa aaccacgacc ctttacgcgt
     1741 gcctgaacca catcaaccgc ccggataaaa aaatcatcac ggtggaagac cctgtggaat
     1801 acgaactctc cggcatcaac caggtcatgg taaaggcgga tatcggcatg accttcgccg
     1861 ccgccctgcg cgccatgctc cgccaggctc ccaacatcat catgatcggg gaaattcgag
     1921 acatggaaac agccagcatc gccatcaacg cctccctgac ggggcacctc gtattctcca
     1981 ccctccacac caatgacgct cccagcgccg tggctcgtct ggcggacatc ggcatcaaac
     2041 gcttcctgat cgcctcctcc gtccgcgcca tcatggctca gcgtcttgtc cgcaagctgt
     2101 gtgaccgctg caaggtggac ggcactctga cggaaaagca ggcgcatacg ctgaacattg
     2161 acatgtcccg ccttgcccag ggccaaatca aggcacccca cggctgcgac ttttgccgcg
     2221 gcggcggatt caagggccga atggggctgt ttgagatttt cgaaatcgac gacgaggtgc
     2281 gccgcatgat taacgaaaac ctgacttccc cccagctgcg ccagcgcgcc cgggaactgg
     2341 gcatgagaac cttgagggaa gacggcgtac gcaaagtgct ggccgggctt acttctccgg
     2401 aagaagtgct gaatgtcacc atgggagacg ccaactgatt tttccccgct attcattctg
     2461 cttattatgg acaccaacct cacactggaa cttttcatcg gccggggaat gattgacaaa
     2521 tccctggcaa aggacatcaa ggaggaaatg atcgcctccg gcaaggaact gccggaagtg
     2581 cttgcagact tcggcatcat cggcagcaag gatgatatct ggcagatgat tgccagcgac
     2641 ctgggtacgg aattcattac actggacaac ttccagccgg acccgaacgt gcagaacatg
     2701 atgccggcca cgctcgtgcg cctgcacggg gcgctccctg tgcggcacgg tccggaaggc
     2761 ctgtacgtct gcctggtgga tcccctgaat cctcagacgg tggaagacct gcgcttcgcc
     2821 ctcggccagg acatccatgt tctggtagcg ccggattacc agatttccga acgcatcaat
     2881 gagctctatg gaggcgaatc cgccgccatg tccgacctga tgcaggaact gaacaacatg
     2941 caggtcaaca atgagacgga ggattctgcc gccgctcccg tcatccgctt tgtagacctc
     3001 gtcattacac aggccatcaa ggaaaaggcc tccgacattc acttcgaacc ttttgagaag
     3061 gaattcaaaa tccgctaccg tgtggacggc gccctgtatg aaatgcagcc tccccccgtc
     3121 cacctgtccg tgccggtcat ttcccgcgtc aaggtcatgg caaacatgaa catcgcggaa
     3181 cgccgcattc cgcaggacgg ccgcatcgtc aagcagatag gaaaccgttc cgtggacatg
     3241 cgcgtttcct cccttcccac tcagtacgga gaatccgtgg tgctccgcgt tctggaccgc
     3301 tcttccgtca acttgaacat ggacaacctg gggcttcccg cgcatatcca cgaatatatt
     3361 ctggatacgg tccacaagcc caacggcatt ttcatcgtta ccggccccac cggcgccggc
     3421 aagacaacta cgctgtatgc cgccctgcgt gaaatcaata ccattgattc caaggtgctg
     3481 acggcggaag accctgttga atacgatatt gacggcatca tccagattcc tatcaatgaa
     3541 gccatcggcc tggatttccc aatggttctc cgcgccttcc tgcggcagga cccggaccgt
     3601 attctggtag gggaaatgcg agacatggca acagcacaga tcgccatcca ggcatccctg
     3661 acgggccacc tggttctctc caccctgcac acgaacgact ctgccggagc cattacgcga
     3721 ctggtggaca tggggtgcga acctttcctg gtagcggcct ccctggaagg ggtgcttgca
     3781 cagcgcctgg tgcgcaccat ctgtccggac tgccgcaccc cgtatgaacc ctcatccacc
     3841 atcctctccc agcttggcgt atctccctat gaattggggg acaagcactt tttcactggc
     3901 cgcggctgtg ataaatgctc cagttccggc tacaggggcc gcaaggggat ttatgagctt
     3961 ttggatatta acgacaccct gcgtgacatg attacggatc gcgctccctc cgtggtgctg
     4021 aaacagaagg ctattgaaat gggaatgtcc acgctgagag aagacgggct gagaaatatt
     4081 tatgacggca acactactat tgaagaagtg ctgaaatata cttaatggac agccggacgc
     4141 cattgcttcc ccacttattt caatcaccaa gaaaaaacac ttaagaaaca acatatatgc
     4201 ctaaatatca atacacagca cttgaccata aaggcgacca gaaaacaggc tccctggagg
     4261 ccaactccga agcggaggcc atggaagcca tccgggcgca tggcctgtac cccacccaga
     4321 tcgtagaagc gggcaagggc aagattcaac agacgcctgc cgccaagaaa aaggccaagg
     4381 gggccaagaa gcaaaaaggc aaactgggag gcaaaatcaa ggccaaggct ctgatgattt
     4441 tcacccgcca gcttgctact ctgattgacg cagggcttcc cctgctccag agtctgaacg
     4501 tgctggccaa acaggaggca aaccccaacc tgcgcgtaac tatcgaagct ctgggagatt
     4561 ccgttcaggg cggctccacc ttctcggaag ccctggccca acaccccaga atctttgacc
     4621 gcctgtttgt caacatggta aaggccgggg aactgggcgg tgtgctggaa gtcgtgctga
     4681 accgtctggc ggaataccag gaaaaggctc aaaagctgaa aagcaaggta atctccgcca
     4741 tggtgtatcc ctccatcgtc ctgtttatcg ccgtaggcat cgtgatcttc ctgatgctgg
     4801 tcatcgtgcc caaattcaag gcgatgttcg cagagcagaa tcaggaactt cccggtattt
     4861 ccgagtttgt gttcggcatc agcgactggt tcatggccgc ccccttcttt gtgcccaatg
     4921 ccgtcattct ggccgctgtg gtcgccatcc tgtacgctgt tttcacggcc atgagcaaga
     4981 cgcccaacgg acgccgcaag attgactccg ctctgctgac catgccggtc atcggcaatg
     5041 tgcagagcaa aagcgccatt gcccgcttcg cccgaacctt cggcacgctg gtgacttccg
     5101 gcgttcccat cctccaggcg cttaccatca cgaaggatac cgccggcaac atgatcgtgg
     5161 gagacgccat cggcctcatc catgactccg tcaaggaggg cgaatccgta gttacgccca
     5221 tgtcctcctc caagcttttc ccgcccatgg taatctccat ggtggacgtg ggggaagaaa
     5281 ccggccagct gccggacatg ctcctgaaaa tcgcggacgt gtatgatgat gaagtggaca
     5341 atgccgtggg agctatgacc tccatgctgg aacccatcat gatcgtattc ttggccgtgg
     5401 tcgtgggcgg catcgtgttc gccatgttcc tgcccctcct gcaggttatt gaaaagatgg
     5461 ggtaacccct caagcagaat cagatgaaaa atcattccgc ataccgtcgg ctccgggaaa
     5521 caaaggggtt caccctcatg gaaatcctgg tagtgatggt gatcatcgtg gtgctggcca
     5581 cgctgaccat ctccatttac acctggcttg aaaccagaaa gaacgaacag gctacggaat
     5641 ccattgtccg caaaattgaa atggggttgg agagctacca cagcgaccat gaccgctacc
     5701 cctacggcac ggaagctccc ttcaataatc acggcgtagc cgtggccaac ggggaggaat
     5761 acagttccaa cgtggtttac atggctctgt tcggcgacca taagaatgag ggtgttcctt
     5821 ccagggatac aaccatttac aatgatgagt tgaacccctc tacgcagccc aaaagcaacc
     5881 cgactgtccg ggaagtccat gtgaaaagca aggacggcag gccggttacc ctttatattc
     5941 ttgcggatcc atggggttcc ccctaccgct accgcctggg cagcgagcag tccatcccca
     6001 ccagaaccga cagggccaaa aacctgaaaa tgggcaacgg cctgaatccg gattatgatt
     6061 tctggtcttt cggaaaagac ggggatagtg atttgaaaga tccgcacgcc tctgaaaacg
     6121 aagatgacat cggcaacctg ccgaagttct gaccttaccg gtatttttcc gcgcacggct
     6181 tcttttccgg cagccttcct ccccttccag tggaggaagg ctttgtgcat ggagggacta
     6241 ttttctggca tccgcacggc ttctgaagta tgatttgggc actgacatga aacgcatttc
     6301 cctgatcgtt ccctgcctga atgaagaaga agccattccg gcgttcaagc aagaaataga
     6361 ccgggtcttc aaggaatgcc tggagggcta cgccccggaa cttatcttcg tggacgacgg
     6421 ttccacggac cgctccctgg aaatgattaa gaaaatggcg gaacactccc ctgaagtaaa
     6481 attcatttcc ttctcccgca atttcgggaa ggaagccgcc atctatgcgg gcctggaagc
     6541 ggccacaggg gattatgccg ccgtcatgga tgtggacctt caggatcctc cttccctgct
     6601 cccggatatg ctgaaagcca ttgagaagga aggatatgac tgtgcgggga cgcgccgcgt
     6661 cacccggcag ggggaaccgc tgctgcggtc ctttttcgcc cgcgcctttt ataagacgat
     6721 tcatcaaata tcggacaccc gcatcgtgga cggagcccgg gactacaagc tgatgacccg
     6781 tcccgtactg gaagccctgc tttccctgaa ggagtacaac cgattttcca aggggctgta
     6841 cgaatgggtg gggttccgga ccaaatggtt tgaatatgaa aatgtggaac gggtggcagg
     6901 agaaaccaag tggtctttct tcaagctgtt cctgtactcc attgaaggta tcgtggcctt
     6961 ttccaccgcc cccctggcgc tggcctccat catgggggta cttctttcct tcatctcatt
     7021 cctttccatc attatcttga cggtccgcga attgatctgg ccccattccg cttacggatg
     7081 gacttccctg gtatgcgtct tctgcatgat gggtggcatc gtgctgctgt gcctgggcat
     7141 tttgggccaa tatctggcca aaacctatac ggaagttaaa aagcgcccta tttacatcgc
     7201 cagggaacgt tccatcaagc ctttcaacta accttgccta tggacacaga accggatttc
     7261 agcattattg cgccca
//
LOCUS       DAOMSU010000096         7118 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_826_length_7118_cov_2.436500, whole genome shotgun sequence.
ACCESSION   DAOMSU010000096 DAOMSU010000000
VERSION     DAOMSU010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7118)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7118
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_826_length_7118_cov_2.436500"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3016
                     /locus_tag="PK077_05465"
     CDS             <1..3016
                     /locus_tag="PK077_05465"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRN23783.1"
                     /translation="WEGRNGSGRVQTDNRGRLDSGTLEVPPIPSGGEVQISLNRKFPG
                     VKPWTAETPNLYKVTYSLNGKDTRSVNIGFRSVEIADNGAVLINGRAVKFKGVNRHEA
                     HPDYGRAIPREVMEKDVQIIKAHNINTVRCSHYPNHPYFYELCDRYGLYVMDEANCEA
                     HGIRNSSMDISRKPSWKKAHVERNMSMVHRSKNHPSIVFWSLGNESGNGPNFEAAAAA
                     IRAYDTTRPLHYCEFPHGHKAVDMDSAMYPPVDRVENWGKQKTSRPFFVCEYAHSMGN
                     ALGNFKEYMDAFESSPRMVGGAIWDFVDQSLRANPAGNGIYKPAPFKGVTQAYGGMFG
                     DRPNQANFCDNGIILGNRNTTAKTKEVKKVYQYMAFERKDGSLSVRNKYFHKPLKGYT
                     LYLVSLVPGGGHAVERMVLPEVPPGKSATVNLPSAAMRTGSLMVLADARFKLPEDVKE
                     LSAKQLEHVVNECEAYEWFPASAEKEVSVPPPAALPAVSVLQGDSVVVQGKGFSASFK
                     NGMLSSLRYGGRDLILPGYPVELQAYRSPVDNDAWIRSKVEGKMKMQNMKAEYSDVKA
                     AVISPGVARITAQFRTKGSELAFSGEVAWTVFGNGIINASVRMYPSAKGEELLRLGVT
                     FGMPAAYDRVEYLGLGPWDNYRDRRTSCWKDIFRTSVDDMFFAYSRPQDMGNRMETDW
                     IALSKPQEDQALWVGASSPRAPLEVSVLRYTPKELNDARSLDRLPEKNKVIVNLDAFQ
                     MGLGGSSCGPRPLAKYQTLSGATALGFVLAPSSALLNLARTGLVVPHSPVIERDGDGM
                     VSLTSSTPEAEIKYSVNKGPEKTYRNPFKLPEGEVRAWAASVKSGKVPSTSPGERKFP
                     LVKGQGEWKILSASSEEPDTGFAHFAIDGNPDTCWHTSYTNGLPGFPHSIAVDMGTRM
                     KFTGFIYTPRMDKDKGLISQYTFSVSDDGQNWREVKKGQFSYHYIRKDPAVQRIDFGR
                     PVEARYFKLDARSPVRSGEQNATVAELNIITQ"
     gene            3170..5746
                     /locus_tag="PK077_05470"
     CDS             3170..5746
                     /locus_tag="PK077_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRN23784.1"
                     /translation="MRKMMACMLGAALCFSMAGAETGVSSAEKASSPFGRMIWNRGWE
                     FRLEDEPGKSGWQTVHLPHTFSLPYFMSDSFYTGLGSYRKKLVMPEEWQGKKVFLDCG
                     AAFQVAEVKVNGKTAGRHEGGYTAFRSDLTPFLKPGENWVEVRVDNRWSPRIAPRAGE
                     HTFSGGLYRNVHLHVCSPVYLPAHGVWVQTPEVTAARGKVRILTEVKNDTGIVRKVTV
                     RHTVREEQSGRVVLRGEKDAELRAGEESRVQADLPPLASPRLWSPAAPNMYRVTTELL
                     DGKGQVLDRAENPLGFRTLELTADRGKLINGKPVYLQGANVHQDHAGWGDAVTDAGAR
                     RDVRLMKNAGFNFIRGSHYPHSQAFLDACDREGMCMLNEGVFWGMGGFKEHDRYWNCD
                     AYPAEKKDRIAFEESCMRQVREMVLQFRNHPSIVIWSISNEPFFTRHAPEARALCNRL
                     ITLVKELDPTRPVCAGGGQRGNFDKLGDMAAFNGDGSHVKTPGRPSMVTEYGSVSCRR
                     PGMYAPGWGDMKKDKEAGIRYPWRVGEAVWCGFDHGSIWPSGGRMGIVDYFRIPKRAW
                     YWYRNALRNIPPPEWPVEGTPAQVKLSADKKVINPADGTDDVYVTVKVADAAGRQISN
                     AVPVTLTVESGPGEFPTGKSITFTPGTDIDLIDGCAAIEFRSYYAGKTVIRASSPGLK
                     GDSLQIVCRNAPAYVAGRSAETRERPYKRFSAKERDIQLARYGRPESGEKANLAVLRP
                     CSASSGFQEAMKASDGDDVSAWHPSEEDKAPWWQLDMEFEFRLDRVEVKAAGSWKGPA
                     PAVQVSRDGNSWKDIKAVLRKDGTELTAMCPEGTGARYVRIRLSPGQGIAEVAVWPAD
                     AS"
     gene            complement(5741..>7118)
                     /locus_tag="PK077_05475"
     CDS             complement(5741..>7118)
                     /locus_tag="PK077_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRN23785.1"
                     /translation="KKDDTPGLFSAFADRPLADRLRPSALEDIMGQDHLLGEGGPLRL
                     MADSGKMTSFILWGPPGCGKTTLARILATRTSMHFAALSAVFSGMADLRKAFDEAARR
                     REYGHGTLLFVDEIHRFNRAQQDGFLPYVENGTVTLVGATTENPSFELNSALLSRCKV
                     FVMHSLDAPALESIIERAETLLQRELPLAPEARATLIELADGDGRYLINLMESVFDLC
                     KPDEMLGTDALLKIVQQRAPVYDKDREGHYNLISALHKSLRGSDTDAALYWAARMLEG
                     GEDPLYLLRRLTRFAMEDISLADPAALQMAIAAWDTYERLGSPEGELAIAELIIYLGC
                     APKSNSAYTAWGAARKAAREYGSLMPPAHILNAPTKLMKELGYGKNYAYDHDAPDAFS
                     GQNYFPDNMPRRRFYRPPERGFEREINKRLAYWDKLRRQRTEEDAGNSGTRSNRKKPP
                     VPEKREPS"
BASE COUNT         1433 a         1876 c         2293 g         1516 t
ORIGIN      
        1 ctgggaggga aggaacggtt ccggccgtgt gcagacggat aacaggggaa ggctggattc
       61 cggaacgctg gaggttcccc ccattccctc cggaggcgag gtgcagattt ccctgaacag
      121 gaaatttccc ggtgtgaagc catggacggc ggaaacgccc aatctgtaca aggtgactta
      181 ttctctgaat ggaaaggata cccgttccgt caatatcggg ttccgttccg tggagattgc
      241 ggataacggc gccgtgctga ttaacggcag agccgtcaag ttcaaggggg tcaaccgcca
      301 tgaagcccat cctgattacg ggcgcgccat tccgcgggaa gtgatggaaa aggacgtgca
      361 gattatcaag gcgcataata tcaataccgt ccgttgttcc cactatccca atcatcctta
      421 tttttatgag ttgtgcgacc gctacggcct gtacgtgatg gacgaagcca actgtgaagc
      481 ccacggcatc cgcaatagca gcatggatat ttcccggaag ccttcctgga agaaagccca
      541 tgtggagcgc aatatgagca tggtgcatcg ttccaagaac cacccttcca ttgttttctg
      601 gtccctgggc aatgagtccg gcaacggtcc caattttgag gcggcagcgg cggctatcag
      661 ggcttatgac actacgcggc ccctccatta ctgcgaattt ccgcacggcc acaaggccgt
      721 ggatatggat tccgccatgt atccgcccgt ggaccgggtg gaaaactggg ggaagcagaa
      781 aacttcccgc ccctttttcg tgtgcgaata tgcccactcc atggggaatg ccctgggtaa
      841 tttcaaggag tacatggatg ccttcgagtc ttccccgcgc atggtgggcg gagccatttg
      901 ggatttcgtg gaccagtccc tccgcgccaa tcctgccgga aacggcattt acaagcccgc
      961 ccctttcaag ggagtaacac aggcttacgg cggcatgttc ggagacaggc ccaaccaggc
     1021 caatttctgc gacaacggca ttattcttgg caaccggaac accacggcca agacgaagga
     1081 ggtgaagaaa gtataccagt acatggcttt tgagcgcaag gacggttccc tgtccgttcg
     1141 caacaaatat ttccataagc ctttgaaagg ttatacgctt tatctggtat cccttgttcc
     1201 cggaggcggc catgccgtgg agcgcatggt tctgccggag gtcccccccg gcaaatccgc
     1261 aacggttaat ctgccttcag ccgctatgcg gaccggttcc ctgatggttc tggcggatgc
     1321 ccggttcaag ctcccggaag acgtgaagga actttccgcc aagcaactgg aacatgtggt
     1381 gaatgagtgc gaagcttacg agtggtttcc ggcatctgca gagaaggagg tttccgtgcc
     1441 tcccccggcg gcgctgcctg ctgtttccgt gcttcagggg gattccgtgg tcgtgcaggg
     1501 aaaaggtttt tccgcctcct tcaaaaatgg gatgctttcc tccctccggt atggcggccg
     1561 ggatttgatt ttgcccggct atccggtgga gctccaggcg taccgctctc cggtggataa
     1621 cgatgcctgg atcaggagca aggttgaagg gaaaatgaag atgcagaata tgaaagcgga
     1681 atattccgac gttaaagctg ccgtcatttc ccccggcgtg gcccgaatca cggcacagtt
     1741 ccggacgaaa ggttccgagc ttgccttttc cggggaagtc gcgtggacgg ttttcggcaa
     1801 tggcattatc aatgcttccg tccgaatgta tccctccgcc aagggagaag aactcctccg
     1861 gctgggcgtc actttcggga tgcctgccgc gtatgaccgg gtagagtacc tggggctggg
     1921 accgtgggat aattaccggg accgccgcac gagctgctgg aaggacattt tccgcacttc
     1981 cgtggacgat atgttttttg cttattcccg gccccaggat atgggcaatc gcatggaaac
     2041 ggactggata gccttgtcca aaccccaaga ggaccaggct ctgtgggtgg gagcctcctc
     2101 tcccagggct ccgctggagg tttccgtcct gcgttatacg ccgaaggagc ttaacgatgc
     2161 caggagtctg gacaggctgc cggagaaaaa taaagtgatt gtgaatctgg atgctttcca
     2221 gatggggctg ggtggctctt cctgcgggcc ccgcccgctg gctaagtacc agacattgag
     2281 cggggccaca gccctggggt ttgtgctggc tccctcgtcc gccctgctga atctggcgcg
     2341 cacggggctg gtcgttcccc attcccctgt cattgaacgg gatggggacg gcatggtcag
     2401 tctgacttcc tctacgcccg aggcggagat aaagtattcc gtcaacaagg ggccggaaaa
     2461 aacgtaccgg aatcccttca agcttcccga aggggaagtg agggcctggg ctgcctccgt
     2521 gaagtccggc aaggttcctt ccacgtctcc cggagagagg aagttccccc tcgtcaaggg
     2581 gcagggcgaa tggaaaattc taagcgcttc ttcggaagag ccggataccg gtttcgccca
     2641 ttttgccatt gacgggaatc cggatacctg ttggcataca tcctatacga acggcctgcc
     2701 ggggttcccc cactccattg ccgtggatat gggaaccaga atgaagttta ccggatttat
     2761 ctacacgccc agaatggaca aggacaaggg cctcatcagc cagtacacgt tttccgtttc
     2821 cgatgacggt cagaattgga gggaagtgaa aaaaggccag ttctcctacc attatatccg
     2881 gaaggatccg gctgttcagc gcatcgactt cggcaggcct gtggaggcac gctacttcaa
     2941 gcttgacgcc cggtctccgg tgaggagcgg ggaacagaac gccactgttg cggagctgaa
     3001 tatcattacc cagtaacgga gcggcaggcg ttttgcactg cttcccggat ggagaagttt
     3061 tccagccgtg agccttttcc ggggtgcacg gcttgttgtt gcgggctttt cagggtgcgg
     3121 aaatgttttt taaatgaaag aaagagcatg gcggaatggt aacatacgca tgcgcaagat
     3181 gatggcctgc atgctgggcg ctgccctgtg tttttccatg gccggagcgg aaacaggcgt
     3241 ttcctctgcg gaaaaagctt catccccgtt cgggaggatg atctggaatc gcggctggga
     3301 gttccggctt gaggacgagc ccggaaagag cggctggcag acggttcatc tgccccatac
     3361 gttcagtttg ccctacttta tgtccgattc cttttacacg gggctcggct cctaccgcaa
     3421 gaagcttgtc atgccggagg aatggcaggg gaagaaggtg tttctggatt gcggcgcagc
     3481 tttccaggtg gcggaggtga aggtgaacgg gaagactgcg ggaaggcatg aagggggata
     3541 tacggccttc cggtcggatt tgacgccttt cctgaaaccc ggcgagaatt gggtggaggt
     3601 acgggtggat aaccgctgga gcccccgcat tgcgccgcgg gcgggggagc atactttttc
     3661 cggaggactg taccggaacg tgcatctgca cgtgtgttct cccgtgtatc tgcccgcgca
     3721 cggagtatgg gttcagacgc cggaggtgac ggcggcccgc gggaaggttc ggattttgac
     3781 ggaagtgaag aatgataccg ggatcgtgag gaaagtgacg gtgcggcata ctgtgcggga
     3841 ggagcaaagc ggccgcgtgg tgctgcgggg agagaaggac gcggaactga gggccgggga
     3901 agagtctcgc gtacaggctg acctgcctcc tctggcttcc cccagattgt ggagccccgc
     3961 ggcccccaat atgtaccgtg tgacgacgga attgttggac gggaaggggc aggtgctgga
     4021 ccgggctgaa aatcccctgg gtttccgtac tctggaactg acggcggacc ggggcaagct
     4081 gattaacggg aaacctgttt atctgcaggg ggccaatgtg caccaggatc acgccgggtg
     4141 gggggatgcc gtgacggacg ctggggcccg ccgtgatgta cggctgatga agaacgccgg
     4201 gtttaatttc atccgcggct cccattaccc tcattcccag gcttttctgg atgcttgcga
     4261 ccgggagggc atgtgcatgc tgaatgaagg cgttttctgg ggaatgggtg ggttcaagga
     4321 gcatgacagg tactggaatt gcgatgccta tccagcggaa aagaaggacc gtattgcgtt
     4381 tgaggaaagc tgcatgcgcc aggtgaggga gatggtgctg caattccgca accatccttc
     4441 catcgtcatc tggagcatca gcaatgaacc gttttttacc aggcatgcgc cggaggccag
     4501 ggctttgtgc aataggctca ttaccttggt gaaggagttg gacccgacac gccccgtgtg
     4561 cgccgggggc gggcagcgcg gaaattttga caagctgggg gacatggccg cctttaacgg
     4621 ggacggctcc catgtgaaga cgcccggcag gcccagcatg gtgacggagt atggttccgt
     4681 gagctgccgg aggccgggga tgtatgctcc tggctggggg gatatgaaaa aggataagga
     4741 agcggggatt cgctatccct ggcgtgtggg agaggcggtc tggtgcggct ttgaccacgg
     4801 cagcatctgg ccttccggag ggcgcatggg cattgtggat tacttccgca ttcccaagcg
     4861 ggcctggtac tggtacagga acgcattgag gaatattcct cctccggagt ggcctgtgga
     4921 ggggactccg gcacaggtga agctgtccgc ggacaagaaa gttatcaacc cggcggacgg
     4981 cacggatgac gtgtatgtga ccgtgaaagt ggcggacgcc gccgggaggc agatatccaa
     5041 tgccgtgccg gtaacgctta cggtggaatc gggacccggg gagttcccca ccggcaaaag
     5101 cattacgttc acgcccggaa cggatattga tttgattgac ggctgtgcgg cgattgagtt
     5161 ccggtcttat tatgccggga agacagtgat cagggcgtct tctcccggat tgaaggggga
     5221 tagcctccaa atcgtttgcc ggaatgctcc ggcttacgta gcgggcagga gcgcggaaac
     5281 gcgggaaagg ccctacaagc gttttagcgc aaaggagagg gatatccagc tggcgcggta
     5341 cggacggccc gaatccggag agaaagccaa tttggctgta ttgaggccct gttccgcttc
     5401 ctccggattt caggaggcga tgaaggcttc cgacggtgac gatgtttccg catggcatcc
     5461 gtccgaggaa gacaaagcgc cgtggtggca gctggacatg gagtttgaat tccgcctgga
     5521 ccgggtggag gtgaaggccg ccggaagctg gaaggggccg gctcccgcgg ttcaggtcag
     5581 cagggacgga aatagctgga aggacattaa agccgttttg cgcaaggatg ggacggagct
     5641 gaccgcaatg tgtccggaag ggaccggcgc caggtacgta cgcatccgtc tgtctccggg
     5701 gcagggaatt gcggaagtgg ccgtatggcc ggcggatgcg tcatgagggt tcccgttttt
     5761 ccggaacagg aggctttttc ctgttactgc gcgtaccgga gtttccggcg tcttcttccg
     5821 tccgttggcg gcgcagtttg tcccaatagg ccaggcgttt gttgatttcc cgttcaaaac
     5881 cccgttccgg cggcctgtag aaccggcggc ggggcatgtt gtccggaaag tagttttgcc
     5941 cggagaaagc gtccggcgcg tcatgatcgt atgcgtaatt ctttccatag cccagttcct
     6001 tcattagttt ggtgggcgcg ttgaggatgt gtgccggggg catcagggac ccgtattccc
     6061 gtgccgcttt ccgtgctgct ccccacgcgg tgtaggcgct gttggatttg ggggcgcagc
     6121 ccaggtagat aatcagttca gcaatcgcca gctctccttc cggagagccc aggcgttcat
     6181 aggtgtccca cgcggcaatg gccatttgaa gtgcggcagg gtccgccaga ctgatatcct
     6241 ccatggcgaa acgggtcagg cggcgcagga ggtagagagg gtcctccccg ccctcgagca
     6301 tgcgtgcggc ccagtacagg gctgcgtccg tatccgaccc ccgcagggat ttatgcaggg
     6361 cgctgatgag gttgtaatgg ccttcccggt ccttgtcgta aaccggggcg cgctgctgga
     6421 caattttcag cagtgcgtcc gtgcccagca tttcatcggg tttgcagagg tcaaagacgc
     6481 tttccatcag gttaatgagg tagcgtccgt ctccgtctgc cagttctatc agggtggcgc
     6541 gcgcttccgg cgcgagcggc agttctcgct gaagaagggt ttccgcccgt tcaatgatgc
     6601 tttccagagc cggggcgtcc agggagtgca tgacaaagac tttgcagcgg gagaggaggg
     6661 cgctgttcag ttcaaaggag gggttttccg tagtggcccc caccagcgta acggttccgt
     6721 tttccacata tggcagaaaa ccgtcctgct gggcgcggtt gaaccgatgt atttcatcca
     6781 cgaacaggag ggtcccatgg ccgtattccc tgcgcctggc cgcttcgtca aaggcttttc
     6841 tcaaatccgc catgccggag aagacggcgg agagagctgc gaaatgcatg gacgtgcgcg
     6901 tcgccagaat gcgcgccagc gtggtttttc cgcagccggg cggcccccac agaataaagc
     6961 tggtcatctt gccggagtcc gccatgagcc ggagaggtcc tccttccccc agcagatgat
     7021 cctggcccat gatgtcttcc agtgcggacg gcctcaggcg gtccgccaga gggcggtccg
     7081 cgaaggcgga aaacaggccc ggggtgtcgt cttttttc
//
LOCUS       DAOMSU010000097         7025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_842_length_7025_cov_2.949928, whole genome shotgun sequence.
ACCESSION   DAOMSU010000097 DAOMSU010000000
VERSION     DAOMSU010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_842_length_7025_cov_2.949928"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..1126)
                     /locus_tag="PK077_05480"
     CDS             complement(305..1126)
                     /locus_tag="PK077_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRN23786.1"
                     /translation="MIQELDLASGERVRFISDIHFGHAKALTREPEELGFLLEGCSHL
                     VVCGDLSETRESPCREEGLEKRARFLRMCRDAGVQPVLLAGNHDPDEKAGLLKLQGGR
                     ICALHGHALFREVAPWGWEYLKNKQISRELIAAFPEAEADLLRRLELARAMSVLVPPV
                     YTRSGTHQNKLVRFLAHSAWPPERPVRILLAWLTMMWRMGKFADRFFPEAEVVIFGHL
                     HRRAVSGKKGRRLYVNLGACFRHARCWAADVTPERGVSIRSYTPGGYDGPEAILR"
     gene            complement(1160..1735)
                     /gene="rlmB"
                     /locus_tag="PK077_05485"
     CDS             complement(1160..1735)
                     /gene="rlmB"
                     /locus_tag="PK077_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRN23787.1"
                     /translation="MRPQGNEQEARRNKHVRSAPSKIAVKALGEEVLDGILDKSPNPL
                     LLILDCVQDPHNLGAILRTADGAGVDAVIAPRDKSVGITETVLRVSVGAAEKVPFIQV
                     TNLARTMKQLQSRGIWIFGTSDKGDRGLYETDFTGPAALVMGAEGEGMRRLTEENCDF
                     LLRIPMKGSIPCLNVSVATGVCLYEMVRQRS"
     gene            1803..3452
                     /gene="nadB"
                     /locus_tag="PK077_05490"
     CDS             1803..3452
                     /gene="nadB"
                     /locus_tag="PK077_05490"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HRN23788.1"
                     /translation="MTWQPASWYNSVPFKMKTSDFLVIGAGVAGLSFALRAAKHGKVA
                     VITKGKFLDCSSAKAQGGIAAVWDRNDSFEDHVADTLDAGAGLCNEQAVRTIVEEGPD
                     AIRELLEWGVNFDPGNQGEDYELAREGGHTKRRILHAKDATGLEITSKLLEAAKLHPN
                     IELLEDHFAIDLITTTKLGLVTEDRVLGAYVLDRVSGEVEIFRSDRVLLATGGCGRVY
                     LYTTNNDSATGDGVAMAWRAGATIANMEFVQFHPTCFFNTRAEGAEARSFLISEAVRG
                     EGGVLIGADGKEFMHKYDPRKSLAPRDITARAVDSEMKRTGSMCVYLDISHKPEGFVQ
                     ERFPLIYETCNRYGVNPAREPIPVVPAAHFQCGGVLTDVNGQTSLDGLYAAGETGCTG
                     LHGANRLASNSLLECVVVAKRALNSMLSLHPLRKDSPTPYDIPAWHHGDTALPDELSV
                     IYHNWDEIRRLMWDYVSIVRTNKRLDRAATRLRMLHREVKDFYWGYRITPDILELRNL
                     VAVASLIVDCAMRRNESRGLHFNTDYPGKLDHRFVTQLHRW"
     gene            3446..4471
                     /locus_tag="PK077_05495"
     CDS             3446..4471
                     /locus_tag="PK077_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23789.1"
                     /translation="MVNYRQRQQALRKGFFGSPLLSACLLTLPVLVSAETARADIQTG
                     RSATRALANSSGRPKGPVIVTPTQRDPLPRVIVKSPVAAEAGKRPAPRILPRTSRQSP
                     GTYPWRLNITATVFWVGEEPTERNPVANHKSSWDSAWRENFGGFDNPDPVCRTRDFRP
                     KTFIPRQNPFYVALPYNDVSRGEHKAEASRVIPWFRREYAGKGQSVCKGRWVQIVYNK
                     RSCFAQWEDCGPFTTEDWPYVFGDKPPVNTQNKGAGIDISPAVRDYLGITGGTAIVHW
                     RFVEFYRIPRGPWSKYGDNNPFVNAGLGAGKKSLQSREDRLRRQQEAIQRELLKDPAK
                     LRRELQG"
     gene            4492..5700
                     /locus_tag="PK077_05500"
     CDS             4492..5700
                     /locus_tag="PK077_05500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRN23790.1"
                     /translation="MQRPRILHIFSRYGEVGGEEICFHAITEALGAIADVTPFVYSTE
                     ELFHSPHGALTKMGYLLHNRDVEQKLRECLRENHYDAWIIHNTFPAMSPCVYELALNQ
                     PAPVIHYMHNYRSGCLNGVFYRDGAPCFSCQGGNYLPGIMRACWRKNAAYSSLAAAVL
                     HQTRRMGTWNRFSSYIAISQRQRELLIQTGIPENKIRVIPHFIRQHPSPAPGAPRRDV
                     LYAGRLTQEKGVLQLVQAWELLSPPGRILYLMGDGPLRGELERYISSRHLESIRLTGF
                     IPHEEQGAVRAACGLSVAPSLWEETFGMVVLESWLHGTPVIVTPNGGLPELITHGRNG
                     WIAQEPSAESLAETLHTALKQEERWPAMGAHGQQLLSSTYSPAAWLRSMEALLGELRV
                     FHSSSTPPTS"
     gene            5916..6902
                     /locus_tag="PK077_05505"
     CDS             5916..6902
                     /locus_tag="PK077_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23791.1"
                     /translation="MILYAWGVGKYLPIARAIHDAGIFLVVYMDTSGLFYPWRYWKTL
                     TLQIWNREKNKRGNLKGPLFACLHIIKNHTFGALDWGRRKHLKMANLISFPSPAALLS
                     ILDRQWLYGKQICKKLALIPNPISSEHTLNPVQPKHNLIVAVGHWDDCFQKNTNLLMQ
                     ALEIFVNKSPSPWIVEIYGRIPPFLHEWHQQLPPQHQNYIHLNGIIPNQKLIKVYAKS
                     KICLCSSRYEGSHIASAEALCAGASVVGPRLTPQLNCLQWYVSHDSGTLSPDDSPESL
                     SGALLEEIRAWDSGKRNPEEISRYWCSLLHAENSCKRIIAAYEAAQGGKTGL"
BASE COUNT         1562 a         2230 c         1818 g         1415 t
ORIGIN      
        1 actttgcttc atggtcagtc ccaattcgga aagaaacggt gaaagaaagt gctccggcgg
       61 cgcacccgtc agaatggagt ccttcttctc caagtttttt tcctcctcac gcccctgctg
      121 ctgccggggc tgcgccctga aaagctccgg gaaaaactgt cccgccggct accagggaaa
      181 acaggcacgg ccaaattcta tggggaacgc gtccacaaac tgtatggact agtcatccgg
      241 tgctctaaca cattatcgcc taaacacgga cgcgttcccc tcactttttt cccgtgggca
      301 ttaatcagcg gagaattgcc tccgggccat catatcctcc gggcgtataa ctgcggatgg
      361 agactcctct ttccggcgtt acgtccgccg cccagcatct ggcatggcgg aaacaggcgc
      421 ccaggttgac gtacagccgc cgcccctttt tcccgctcac cgcccgccgg tgaagatgtc
      481 caaaaatgac gacttccgct tccgggaaaa aacggtctgc aaatttcccc atgcgccaca
      541 tcatcgtcag ccatgccaga agaatccgca cgggccgttc cggcggccag gcagaatgag
      601 ctaaaaaacg caccagttta ttttgatgtg ttccggaacg cgtataaaca ggaggcacca
      661 gcacactcat ggcacgggcc agctccagcc gccgcagcaa atctgcctcc gcctccggga
      721 acgcagcgat cagttcgcgg gaaatctgct tgtttttcaa atattcccat ccccacggag
      781 ccacctccct gaacagcgca tggccgtgca aagcgcagat gcggccaccc tgcaatttca
      841 gcagtccggc tttctcgtcc ggatcatggt tgccggccag cagaacgggc tgcaccccgg
      901 catcccggca catccgcaga aaacgggccc gtttttccaa tccttcctcc cggcaggggc
      961 tttcacgggt ttcactgagg tccccgcaca ccaccagatg ggagcacccc tccagaagaa
     1021 accccagctc ttccggctcc cgggtcagcg ccttggcatg gccgaaatgg atgtctgaaa
     1081 taaacctgac gcgttccccc gaagcaagat caagttcctg aatcataaag acgagggaag
     1141 gagaaaaaca gtcatggaat caggaacgct gccgcaccat ttcatacagg catacgcccg
     1201 tagccacaga tacgttcagg cacggaatgc ttcccttcat gggaatcctc agcaggaaat
     1261 cgcagttctc ttccgtcaat cttctcatcc cttccccttc cgcccccatc accagtgcgg
     1321 ccggtcccgt gaagtccgtt tcatacaggc cgcggtctcc tttgtccgac gtgccgaata
     1381 tccagatgcc gcggctctgg agctgcttca tggtgcgcgc cagattggtc acctgaataa
     1441 aaggcacctt ttccgcagcg ccgacagaaa cgcgcagcac cgtctccgta atgcccacgg
     1501 atttgtcccg gggggcgatg acggcatcca ctcccgcacc gtccgccgtc cgcaggatgg
     1561 ctcccaggtt gtggggatcc tgcacgcaat ccagaatcag cagcagaggg ttgggggatt
     1621 tgtccagaat cccgtccagc acctcctctc ccagcgcctt cacggcaatt ttggacggcg
     1681 cgctgcgcac atgcttgttc cggcgcgctt cctgttcgtt cccttgaggt ctcatgccca
     1741 tgaattagcg tgtttcacac ttcttttcca gacaaatatg cccttccgcc ccatcattgg
     1801 gcgtgacttg gcagcccgcg tcatggtaca attccgtacc attcaagatg aagacgagcg
     1861 actttttagt cattggggca ggtgttgccg gattgagttt cgccttgaga gcggcgaaac
     1921 acggcaaggt tgcagtcatt acaaaaggca aatttctgga ttgcagttcc gccaaggcac
     1981 agggcggcat cgccgccgta tgggaccgca acgattcctt tgaagaccat gttgcggata
     2041 cgctggacgc cggagcgggc ctctgcaacg aacaggccgt gcgcactatc gtggaggaag
     2101 gcccggacgc tatccgggaa ctgctggaat ggggagtgaa ctttgacccc ggcaaccagg
     2161 gagaggatta tgaactggcc agggagggcg gccacaccaa acggcgcatc ctccacgcca
     2221 aagacgcaac cggactggaa attacctcca aacttctgga ggcggccaag cttcacccca
     2281 acattgaact gctggaagac catttcgcca ttgacctgat tacgaccacc aagctgggcc
     2341 tcgtcacgga agaccgcgtt ctgggagcct acgtgctgga ccgcgtctcc ggggaagtgg
     2401 aaatcttccg gtcagaccgc gtgctgctcg ccaccggcgg ctgcgggcgc gtatatctgt
     2461 acactaccaa taatgattcc gccacggggg acggcgtagc catggcctgg cgggcggggg
     2521 ccaccatcgc caacatggaa ttcgtgcagt tccatcccac ctgtttcttc aacacccgcg
     2581 cggaaggcgc ggaagcgcgc tccttcctca tctccgaagc cgtgcgcggg gaaggcggcg
     2641 tgctgatagg cgcggacggg aaggaattca tgcacaaata cgatccccgc aaatctctgg
     2701 ccccgcgcga catcaccgcc cgcgccgtag attcggaaat gaagaggacg ggcagcatgt
     2761 gcgtttatct ggatatctcc cacaagccgg aagggttcgt ccaggaacgt tttcccctga
     2821 tttacgagac atgcaaccgc tacggcgtca atcccgccag ggaacctatt cccgttgttc
     2881 ccgcagcgca tttccagtgc ggcggcgtgc tgacggacgt caacggccag accagcctgg
     2941 acggccttta cgccgcaggg gaaaccggct gcaccggtct tcacggcgcc aaccggctgg
     3001 cttccaactc ccttctggaa tgcgtggtgg tcgccaagag ggcgctgaac tccatgctct
     3061 ccctgcatcc cctgagaaag gattccccca ctccttacga catccccgcc tggcaccacg
     3121 gagatacagc cctgcctgac gaactttccg tcatctatca taattgggat gaaatccgcc
     3181 gcctgatgtg ggattacgtc tctattgtgc ggaccaataa aaggctggac cgcgcagcca
     3241 cgcggctccg gatgctgcac cgggaggtaa aggacttcta ctggggatac cgcattacgc
     3301 cggacattct ggaattgcgc aacctggtgg cggtggcctc cctgatcgta gactgcgcca
     3361 tgcgccgcaa tgaaagccgc ggccttcatt tcaatacgga ttatcccggc aagctggacc
     3421 accgcttcgt cacccaactg caccgatggt aaactaccgg cagcgtcaac aggcattacg
     3481 gaaggggttc ttcgggagcc ccctcctgtc cgcatgcctg ctcacgctgc cggttctggt
     3541 ttccgcagaa accgccaggg cggacatcca gacaggccgg agcgccaccc gcgccctggc
     3601 gaacagttcc ggacggccca aaggccccgt catcgtcacc ccaacgcaac gggaccccct
     3661 gccccgggtc atcgtcaaat cacctgttgc tgcagaggca gggaaacggc ccgcgccccg
     3721 catccttccc aggacgtcca ggcaaagccc cggcacctat ccctggcgtc tcaacattac
     3781 cgccacggtc ttctgggtgg gggaagaacc cacggaacgc aatccggtag ccaaccacaa
     3841 aagttcgtgg gattccgcat ggcgggaaaa cttcggcggc ttcgacaacc cggatcccgt
     3901 ttgccgcacg agggacttcc gcccgaaaac attcatcccc cggcaaaacc ccttttacgt
     3961 ggcgctccct tataatgacg tttccagagg ggagcataag gcggaggcgt cacgggtcat
     4021 cccgtggttc cgccgcgaat atgcgggaaa ggggcaatcc gtctgcaagg gcagatgggt
     4081 gcaaatcgtc tacaacaagc gttcctgttt tgcccaatgg gaagactgcg gccccttcac
     4141 cacggaagac tggccctacg tctttggaga caagcctccc gtcaataccc agaacaaagg
     4201 ggcgggcatt gacatctccc ctgccgtacg cgactatctg ggcattacgg gaggaacagc
     4261 cattgtccac tggcgctttg tggaatttta ccgcattccc cgcggcccgt ggtccaaata
     4321 cggggacaac aatccctttg tcaatgccgg gctgggagcc gggaaaaaat cccttcagtc
     4381 acgggaagac cgtctgcgcc ggcagcagga agccattcag cgggagctat tgaaggatcc
     4441 cgcgaaactc agaagggaac tccagggata accccctgtg ccgccgcagc catgcaacgt
     4501 ccacgtattc tacatatttt cagccgttac ggcgaggttg ggggggaaga aatctgcttc
     4561 catgccatta cagaggcttt gggtgccata gcggatgtca cgccctttgt ctattcaacg
     4621 gaggagctgt tccatagccc ccacggcgcc ctgacgaaaa tgggttattt gctccacaac
     4681 agggacgtgg agcaaaaact gcgcgaatgc ctgcgtgaaa accactatga cgcatggatc
     4741 atccacaata cgttcccggc catgtccccc tgcgtctatg aactggccct gaatcagccc
     4801 gctcccgtca tccactacat gcacaattac cgctccggct gcctcaacgg ggttttttac
     4861 cgggacggag cgccctgctt ttcctgtcag ggcggcaact atctccccgg catcatgcgc
     4921 gcctgttgga gaaaaaacgc cgcgtactcc tctctggcag ccgccgtcct gcatcaaacg
     4981 cgccgcatgg gaacctggaa ccgcttttcc tcctacattg ccatcagcca acgccagcgg
     5041 gagcttctca tccagaccgg aataccggag aataaaatca gggttatccc gcatttcatc
     5101 cggcaacatc cttcccccgc ccccggagca ccccgccggg acgtccttta cgccggacgc
     5161 ctgacgcagg aaaaaggagt cctgcaactg gttcaggcgt gggaactcct atcccccccc
     5221 ggccgcattc tctacctgat gggagacggc cccctgcgcg gggaactgga gcgttatatc
     5281 tcttcccgcc atcttgaatc catccgcctg accgggttca ttccccatga ggaacaagga
     5341 gccgtccgcg ccgcctgcgg cctctccgta gcgccctccc tctgggagga aaccttcggt
     5401 atggttgtcc tggaatcatg gctccacggc acgcccgtca ttgttacccc gaacggcggc
     5461 ctgccggagc tcatcaccca cggcaggaat ggctggattg cacaggaacc ttccgcggaa
     5521 tccctggcgg agacgctgca caccgccctg aagcaagaag aacgctggcc ggccatgggc
     5581 gcgcacgggc aacaactttt gtcctccaca tactcccccg ccgcatggct ccggtccatg
     5641 gaagcccttc ttggcgagct ccgcgttttc cattcatctt ccaccccacc aacatcatga
     5701 aatggtacac atgcactccg gtgtccttca agggagacca tactttcttt tcccgcgatt
     5761 caggcgcttt ctgcaaggcc tttcagagaa tcggggtgga aagcagggcc atcatgccca
     5821 cacctgccca ggaaggagat gatcccgacc tcatccgcac ggaatacgcc aacctgaaag
     5881 atgcagcctg gtggaagtcc ctgggtattg acggagtgat cctgtacgcc tggggagtgg
     5941 gcaaatacct tcccatcgcc agagccatcc atgacgcggg aatctttctg gtagtgtata
     6001 tggatacttc cgggctattt tacccttggc gctattggaa aacacttaca ctccaaattt
     6061 ggaataggga gaaaaataaa agaggaaatt taaagggccc tctttttgcc tgtctccaca
     6121 tcatcaaaaa tcataccttc ggagctttgg actgggggag aaggaaacac ttgaaaatgg
     6181 caaatcttat ttcctttcca tctcctgctg ccttactttc catcctagac cgtcaatggc
     6241 tttatgggaa acagatatgt aaaaaacttg ctcttattcc caacccgatc tcttctgaac
     6301 atactctgaa tcctgtacag ccaaaacaca atttaattgt tgctgtgggt cattgggacg
     6361 attgtttcca aaaaaacaca aatcttctga tgcaagcact ggaaatattc gtgaacaaaa
     6421 gtccctcccc gtggattgtt gaaatttatg ggagaatacc acccttcctg cacgaatggc
     6481 accaacaatt acccccccaa catcaaaatt acatccattt aaatggaata atacccaatc
     6541 agaaacttat caaagtttat gcaaaatcta aaatttgtct ttgttcctcc cgttatgaag
     6601 gttctcacat tgcctccgca gaagcgctgt gcgccggggc ctctgtcgta gggcctcgcc
     6661 tcacacccca gctgaactgc ctgcaatggt acgtttccca tgattcaggc acactcagcc
     6721 cggacgattc tccggaaagc ctctccgggg cgcttttgga agaaatccgc gcctgggact
     6781 ccggaaaacg caacccggag gaaatatccc gctattggtg ttccctgctg catgcggaaa
     6841 attcctgcaa acgcattatt gccgcctatg aagccgccca aggcggaaaa accggcctct
     6901 aactacccga cagggcatgc gcctcaatcc acacatcctc ccatttcctg tccgccagcg
     6961 ccccatagag aggatggctc cacaaccggg cgcgcatggc cgcgggggaa gccagcccca
     7021 gcaaa
//
LOCUS       DAOMSU010000098         7023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_843_length_7023_cov_2.506889, whole genome shotgun sequence.
ACCESSION   DAOMSU010000098 DAOMSU010000000
VERSION     DAOMSU010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_843_length_7023_cov_2.506889"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1143
                     /locus_tag="PK077_05510"
     CDS             172..1143
                     /locus_tag="PK077_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HRN23792.1"
                     /translation="MLEYRAEGLCRNASHLRREELDHCIAAGEVLQSTALAFDTNRRL
                     RFELCGHHAYMPFEECLDTAPGETVKEIAVLTRVGRPTCFVVQGTTQDESGEAVYLLS
                     RAAAQRACREQYLDQLENGSVIPCTVTHIENFGAFCDVGCGISALLPIDCLSVSRIAS
                     PADRVTVGQQLLCAIKNRDEQDRIVLTLRELLGTWSENAACFAAGETVVMSFTPHLIP
                     VNTGICSTITAKVKKGADPECVGRLLEEAYAAAPFVRLLGRNQPADTKNVTRTNCVDI
                     GWAYDPRTNRVILMSAEDNVVKGAGGQAVQSFNIMCGFDETEGLWVL"
     gene            1153..2430
                     /gene="argJ"
                     /locus_tag="PK077_05515"
     CDS             1153..2430
                     /gene="argJ"
                     /locus_tag="PK077_05515"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HRN23793.1"
                     /translation="MLKDTFKTMNDSSYIPVDGGVCAPRGFLGSAVSCGIKKPTATRL
                     DLALIYSTEPCVSAGTFTTNRVQAACVKVSREHLRKGNIRAIVANSGNANACTGTRGV
                     EDARGECRSVAELLGLKPAEVAVCSTGVIGLPMPMMRIYPKFPELAEGLSRDKGHEVA
                     QAVMTSDTKEKIIAIEFVVQGKPVRIGACCKGAGMINPCMATMLCFITTDAGVSRDVL
                     ELCVQSGVKSSFNCITIDGDMSTNDTVLVMANGASGVKLETPEDIYAFQQALAHVMLE
                     LAKHIVQDGERVTKFVTVHVTGGRTEDEAKKAAEAVAKSSLVKSSWNGNDPNWGRIIH
                     AVGYCGAKVDEEKIDIDIAGLAACRGGVQADTPSDDLRQAVQVPAFQVDINLNRGEFS
                     HTVYTTDLSPEYVDFNRSEYAYWNQAKADGLTC"
     gene            2481..2942
                     /locus_tag="PK077_05520"
     CDS             2481..2942
                     /locus_tag="PK077_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRN23794.1"
                     /translation="MAPSAGMLMEVRRLLDEGAFRASRAQRFCTGAADCCRFRLTGET
                     PHVTLGEAWVAWKAWRASGRTRLELPPDGSCPFLSGQGRCMIYEGRPLACRTHFCVPA
                     GGALPRREVIDLIHALEDIDAALGGDGAARLPEAVERLSRKGPADGRKRRR"
     gene            complement(3080..4249)
                     /locus_tag="PK077_05525"
     CDS             complement(3080..4249)
                     /locus_tag="PK077_05525"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN23795.1"
                     /translation="MFPSAVTCEMNDASHNHGEQENTVSTYSVDVETLQQIALKGDAQ
                     ALFQLAINYEQGRGVAENQQEAFYCYQQAAELGHVTAQLNLGWAYSNGIGAPQDNDKA
                     FYWYRKAAEQGHPTAQFDLGFCYVNGLGVEKDEHQAIGWYKKAAEQGHAVAQLNLGWI
                     YANSPSRKNWEQAVYWYKQAAEQGDPRAQYNLAWCYGNGSGTPKNPRKAAYWYEEAAM
                     QNHATAQYNLGWCYENGFGVEPDLDKALVWYHKSALQGQITAQYTLGWCYGNGRGMEV
                     DMAKAVHWYTKAAEQGHTTAQLNLGWCHLNGKGTPVNREEALKWYLKAAEQGNATAMF
                     NVGNCYAHGYGIEQDDKQAEEWYLKAVRHGNKKAASALRHLASKQEKEKQTDSEA"
     gene            complement(4353..5378)
                     /locus_tag="PK077_05530"
     CDS             complement(4353..5378)
                     /locus_tag="PK077_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23796.1"
                     /translation="MTLRFLSSALAALLLPGTLHAAPVFLENVSESGGWYDCNKKTKW
                     TWGTKPHPTNPLNPPVSARPTEWLYLPYDQNICWAAAASNVLQWWQDTRSDLKSTTPN
                     GKSATYDVMPQVAQLAIYQTISTNWTNSGGSVEQAYNWWVNGGMLDTTAYPSLSTTTA
                     PGGYWKELGLTVPQSSSGGAVDNPLFSAYSFWEGDTRSGVYQKLTNSINNNWGTTLTV
                     GEEGRGHAITMWGYDTDKDGNLIIYLTDSDDYAVEMFRQKVVVNDYTDEYGSSHCDIY
                     LTSLDGENDVYSYNYEEAGLSGIRLGEIQSFTAPLGDLRIPEPGAGILFLCGSFLALS
                     RRRRAGR"
     gene            complement(5550..>7023)
                     /locus_tag="PK077_05535"
     CDS             complement(5550..>7023)
                     /locus_tag="PK077_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRN23797.1"
                     /translation="SLAAALTPLMEEINKRVRFLNELGLSYLSLDRQANTLSGGELQR
                     ARLASQLGGGLSGVLYILDEPTAGLHPADTDRLLRALRTLRNQGNTVLVVEHDEQILT
                     AADYLVDMGPGSGTNGGRILAQGSLAEILENAESPTGEWLSGKRNMPASGRKTAPAER
                     LVLTGADKHNLNNVTLNIPVGTLTCISGPSGSGKSTLVRDCLIPAVRQALSGKKDIPR
                     RVQGTEHFNRLVVIDQSPIGKTPRSTPATATGLLQVLRPLYAQLPLSKQRGYTAARFS
                     PNIRGGRCERCQGTGMIEVDMNFLGNVAMPCDACHGQCYNRETLEITWKGKSIAQALA
                     LTVDEAAEFFSSLPRAAAILKSMQDVGLGYLNLNRRADTLSGGESQRIKIAAELAKAP
                     SWKLAEDGKRALFILDEPTSGLHFNEVALLLAALFRLRDAGHTILCVEHHKDLLNAAD
                     YLVDMGPGAGRHGGNIVAEGSPADVASNPESPTSPWLVPR"
BASE COUNT         1331 a         1875 c         2222 g         1595 t
ORIGIN      
        1 agaattatgc ggtattgcca tagatattat tatataccat cccggcagtt ttgaaaatag
       61 ccctgccggg aaaagctgcc cccgccagga gaatcttttg tgaacttttg gcggtagcgg
      121 cagctatgat cgttgttctt ttattttata tataagaaaa gaggaaaaca aatgcttgaa
      181 taccgtgctg aaggactttg ccgcaacgct tcccacctgc ggcgggaaga gctggatcat
      241 tgcattgccg ccggggaggt gctacaaagc acagcccttg cctttgatac caaccgccgc
      301 ctgcgctttg agctgtgcgg gcaccatgcg tatatgccgt ttgaagaatg tctggacact
      361 gcccccggtg agacagtcaa agagatcgct gtgctgaccc gcgtggggcg gccgacctgt
      421 tttgtggttc agggtacgac gcaggatgaa agcggcgagg ccgtttatct gctttcccgc
      481 gcggcggcgc agcgggcctg ccgggagcag tacctggacc agctggaaaa cggcagcgtg
      541 attccctgca cggtaacaca cattgaaaac tttggcgcgt tctgcgatgt cgggtgcggc
      601 atttcagcac tgctgccaat cgactgcctt tcagtttcgc ggattgcttc cccggcagac
      661 cgtgtaacgg tgggccagca gctgctgtgc gccatcaaga accgggatga gcaggaccgc
      721 attgtgctga ccctgcggga gctacttgga acctggagtg agaacgccgc ctgctttgcc
      781 gccggggaaa cggtggtgat gtcctttacc ccccatctga ttcccgttaa tacgggcatc
      841 tgctccacga ttacggccaa agtgaaaaag ggggcggacc ccgaatgcgt cggccgcctg
      901 ctggaagaag cttatgccgc ggccccgttc gtccgccttc tggggcgcaa ccagcctgcg
      961 gataccaaga atgtaacgcg gacgaactgc gtggacattg gctgggctta tgatccccgc
     1021 acgaaccgcg tgattctgat gagcgcggag gataatgtgg tgaagggggc ggggggccag
     1081 gccgttcagt ccttcaatat catgtgcgga tttgatgaaa cggaaggctt gtgggttctg
     1141 tagctttttg atatgttgaa agacactttc aagacgatga atgattcatc ctatattccg
     1201 gttgacgggg gcgtttgcgc gccccgggga tttttgggga gcgccgtaag ctgcggcatc
     1261 aagaagccta cagccacgcg tctggacctg gccctgattt attccacgga accctgcgtg
     1321 tccgcaggaa cgtttacgac caaccgcgtg caggccgcct gcgtgaaggt gagccgggag
     1381 catctccgga aaggaaatat ccgcgccatc gtagcgaaca gcgggaacgc gaacgcctgc
     1441 accggaaccc gtggggtgga ggacgccagg ggcgaatgcc gcagcgttgc ggagcttctg
     1501 gggctgaagc ctgcagaagt ggccgtatgt tccaccggcg tgattggcct gccaatgcct
     1561 atgatgcgca tttacccgaa gtttccggaa ttggcggagg ggctttcccg tgacaagggg
     1621 catgaggtgg cccaggccgt gatgaccagc gacacgaagg agaaaatcat tgccattgag
     1681 tttgtagtgc agggtaaacc cgtgcgcatc ggcgcctgct gcaaaggggc gggcatgatc
     1741 aatccctgca tggcgaccat gttgtgtttt atcacgacgg atgcgggcgt ttcccgcgat
     1801 gtgctggagc tgtgcgtcca gtccggagtg aagagttctt ttaactgcat caccattgat
     1861 ggagacatga gcacgaatga cacggttctg gtgatggcga atggggcatc cggcgtgaag
     1921 ctggaaactc cggaagatat ttacgcattc cagcaggctt tggcccatgt gatgctggaa
     1981 ctggccaagc acatcgtgca ggacggagag cgggtgacta agttcgtcac cgtgcatgtg
     2041 acgggaggcc gcacggagga tgaagctaaa aaagcggcgg aagccgtagc caagtcttcc
     2101 ctggtgaaga gttcctggaa cgggaacgat cccaactggg ggcgcattat tcatgctgtg
     2161 ggctactgcg gagcaaaggt ggacgaagag aagatagaca ttgacatcgc cggtctggcc
     2221 gcctgccgcg gtggcgtgca ggcggatact ccgtccgacg atttgcggca ggccgtgcag
     2281 gttcccgcgt tccaggtgga tatcaatttg aatcggggcg agttttccca tacggtgtat
     2341 accacggatt tgtctccgga atacgtggat ttcaaccgtt ccgagtatgc atactggaac
     2401 caggcgaaag cggacggcct gacctgttaa ccgtttctgt catggcgcgt ggcaaatgcc
     2461 gccagggaaa ggcgggggga atggctccct ccgccgggat gctgatggag gtgcggcgcc
     2521 ttctggatga aggcgctttc cgcgcttccc gcgcccagcg tttctgtacc ggtgcggcgg
     2581 attgctgccg gttccgcctg accggggaga cgccgcatgt aacgctgggt gaggcatggg
     2641 tggcctggaa ggcgtggcgc gcttctggac gcacccggct ggagctgcct ccggacggga
     2701 gctgcccttt tttaagcggg cagggaagat gcatgattta tgaaggcagg ccgttagcgt
     2761 gccgcactca tttctgcgta ccggccgggg gcgccttgcc ccgccgggag gtcattgacc
     2821 tgattcatgc gctggaagat attgacgcag cgctgggcgg cgatggcgca gcccggctgc
     2881 cggaggccgt ggaacggctg tcccgaaaag gcccagcgga tggaaggaag aggcggcgat
     2941 gatgatccgg gggtgacggc agtcaggcgt ctatttctaa gcggaagcac attttttcag
     3001 gccatgggaa catgttttcc cttcgtgtgt ccatggctgg acctggttag aggatagaag
     3061 taagcaggag aatgcctgtt caggcctccg agtccgtttg tttctctttt tcctgtttgg
     3121 aggccagatg gcgcagggcg ctggcggcct ttttgttgcc gtgccggacg gctttcagat
     3181 accattcttc cgcctgttta tcgtcctgct caatgccgta gccgtgggcg tagcagttgc
     3241 ccacattaaa catggctgtg gcgttgccct gttctgccgc cttaaggtac catttcaggg
     3301 cttcttcccg gttgacgggc gttccttttc cgttcagatg gcaccacccc aggttgagct
     3361 gggctgttgt atgcccctgt tctgcggctt tggtgtacca gtggacggct ttggccatgt
     3421 ccacttccat gccccggccg ttgccgtagc accatcccag cgtgtattgg gccgtaatct
     3481 ggccctgaag ggctgatttg tgataccaca ccagcgcttt gtccagatcc ggctccacgc
     3541 cgaaaccgtt ttcatagcac cagcccagat tgtattgggc cgtagcgtgg ttctgcatgg
     3601 cagcttcttc ataccagtaa gcggctttcc gcgggttttt gggggtcccg cttccgttgc
     3661 catagcacca ggccaggtta tactgggcgc gggggtctcc ctgttccgca gcctgcttgt
     3721 accagtatac ggcctgttcc cagtttttcc ggctggggct gttggcataa atccagccga
     3781 ggttgagctg ggctaccgca tggccctgtt ccgccgcttt tttgtaccac ccgatggcct
     3841 ggtgttcgtc tttttccact cccaggccat tgacatagca gaaaccaagg tcaaattgcg
     3901 cggtgggatg cccctgttcg gcggctttcc ggtaccagta aaaggccttg tcgttgtcct
     3961 gaggggcgcc gatgccgttg gaataggccc atcccagatt gagctgggcg gtaacgtggc
     4021 ccagttccgc cgcctgctgg tagcagtaga aagcttcctg ctggttttcc gccaccccgc
     4081 gcccctgttc gtagttgatt gccagctgga acagtgcctg ggcgtctcct ttcagagcta
     4141 tttgctgaag ggtttctacg tcgacggaat aggtggagac ggtgttttcc tgctcgccgt
     4201 ggttgtggga agcgtcattc atttcacagg taaccgcact ggggaacact gggacaattt
     4261 ttgctctcct gaacttgtgc gctcgccgat tctgcacatt tcccccataa gaatcaagac
     4321 ccgtttttat tggggccgga gttcggaaag cgttaccgtc cagcgcgcct gcggcgggac
     4381 agggcaagga atgaaccgca gaggaagagg attccggcgc cgggttccgg gattctcaga
     4441 tctcccagcg gagcggtgaa gctctgtatt tcccccaggc ggatgcccga cagtcccgct
     4501 tcctcgtaat tataactgta cacgtcgttt tctccatcca ggctggtcag ataaatatcg
     4561 cagtgggagg agccgtattc gtccgtatag tcgttcacga ctactttctg gcggaacatt
     4621 tccacagcgt agtcgtccga atccgtcagg tagataatca gattgccgtc tttgtccgtg
     4681 tcgtagcccc acatggtgat ggcgtggcct ctcccttctt ccccgacggt cagcgtggtt
     4741 ccccagttgt tgttgatgct gtttgtcagt ttctggtaaa cgccgcttct ggtgtctcct
     4801 tcccagaagc tgtatgcgga aaataagggg ttgtcaacag ctcctccgct tgaggactgc
     4861 gggactgtca gtcccagctc tttccagtat cctccgggag ctgtcgtcgt agaaaggctc
     4921 ggataggcgg tggtatccag catcccgccg ttgacccacc agttgtaggc ctgttccacg
     4981 gagcctccgc tgtttgtcca gttcgtggag atggtttggt aaatggccag ctgggcgacc
     5041 tgcggcatga catcgtaagt ggcggatttc ccgttgggcg tggtggattt caggtcgctt
     5101 ctggtatcct gccaccattg gagaacgttg gatgccgccg ccgcccagca gatattctgg
     5161 tcataaggaa ggtacagcca ttctgtgggc cgtgcggaaa ccggaggatt taacgggttg
     5221 gtggggtggg gcttggtccc ccatgtccat ttggtttttt tattgcaatc ataccatccg
     5281 ccggattcgg agacgttttc aaggaatacg ggcgcggcat gaagcgttcc gggaagtaac
     5341 aacgccgcca gagccgaaga gaggaaacgg agagtcatag gaaaaaggaa tacgtaattc
     5401 cttttttgtt aaaaaacagg gaggtcgtca agataataaa taggggaagg agacgaataa
     5461 actccgggag gcagattttt tcccgaagac gccgggaacg ggatgtccgg aggacaggac
     5521 agatgaatga ttttcagggg ctgccgttgt taacggggga cgagccaggg agaagtgggc
     5581 gattccggat tggacgctac atctgcgggg gagccctcgg ccacgatatt gccgccgtgc
     5641 ctgccagcac cggggcccat gtccaccagg tagtccgcgg cattgagcag gtccttgtgg
     5701 tgttccacgc agaggatggt gtgcccggca tccctcagac ggaaaagggc tgccaggaga
     5761 agggccactt cattgaagtg aaggccgctg gtgggttcgt ccagaatgaa cagggccctt
     5821 ttcccgtctt ccgccagttt ccaggacggg gctttggcca gttccgcagc tatttttatg
     5881 cgctgggatt ctccgccgga aagggtgtcc gccctgcggt tgagattgag gtatcccagc
     5941 cctacgtcct gcatgctttt caggatggcg gcggctctgg gcagggagga aaagaattcc
     6001 gccgcttcgt ccacggtcag ggccagcgcc tgggcaatgg atttcccttt ccaggtgatt
     6061 tccagcgttt ccctgttgta gcattgcccg tggcaggcgt cgcagggcat ggccacattt
     6121 cccaggaagt tcatatccac ttcaatcatg cccgttccct ggcaccgttc acaacggcct
     6181 ccgcgaatat tgggggaaaa gcgcgccgcc gtgtatcccc tctgcttgga aagggggagc
     6241 tgtgcgtaaa gggggcgcag cacctggagc aggccggtag cggtggccgg cgtggaacgc
     6301 ggcgttttgc cgatgggcga ctggtcgatg acgacaaggc ggttgaagtg ctccgttccc
     6361 tgcacgcggc gcggaatatc ctttttcccg gaaagggctt gcctgaccgc ggggatgaga
     6421 cagtcccgga caagggtgga tttccctgaa ccggaagggc cggagatgca ggtcaatgta
     6481 ccaaccggga tattcagagt gacattgttg aggttgtgtt tgtccgctcc ggtcaggacc
     6541 agacgctccg caggagccgt cttgcgtccg gaggcgggca tatttcgctt gcctgaaagc
     6601 cattctccgg tggggctttc ggcattttcc agtatctccg caagggagcc ctgtgccaga
     6661 atacggcctc cgttggttcc ggagccgggg cccatgtcca ccaggtaatc cgccgcggtt
     6721 agaatttgct catcatgctc tacgaccagg actgtgttgc cctggttccg tagcgtccgg
     6781 agagcgcgga gcaggcggtc cgtatcggcg gggtgcagtc cggccgtggg ttcgtccagg
     6841 atgtaaagga ctccggaaag gccgcctccc agctgggaag ccaggcgcgc cctctgcagt
     6901 tcgcctccgg aaagggtgtt tgcctggcgg tccagagaca ggtaggagag ccccagctca
     6961 ttcaggaagc gcacgcgttt gttgatttcc tccatgagcg gggtcaatgc cgccgccagg
     7021 gag
//
LOCUS       DAOMSU010000099         6939 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_870_length_6939_cov_2.467170, whole genome shotgun sequence.
ACCESSION   DAOMSU010000099 DAOMSU010000000
VERSION     DAOMSU010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6939
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_870_length_6939_cov_2.467170"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PK077_05540"
     CDS             <1..356
                     /locus_tag="PK077_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23798.1"
                     /translation="FSVCKMAQAMNCASDEKREKALNIPLWRRCACISGISAAAVVGA
                     VMLVQHALVAPVGTVTAGNAGEVPSEKAETPVIPGEKRELLRTPTLFELQGGDERVPV
                     IAPAVIRKILPEKEY"
     gene            548..1501
                     /locus_tag="PK077_05545"
     CDS             548..1501
                     /locus_tag="PK077_05545"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRN23799.1"
                     /translation="MKTCIFLTLGLLVGSGSGYSIDRPAGSTDNLQPPPLTPYAHQVK
                     PLPSSKPARLGIVPGTVPQALVAQLELSGFPGVLVTKVMPDSPAAKAGLQENDVMVKL
                     GDVSLSGPQSVTEALSEKVPGDRITAVFYRKGKRETVEITLDGGTLSAEEILAAQGDP
                     RTQPRAVPSVRRQTAPFSGMAARPNLPQRILDMQQMMDEFLKDSAMDDYRMDDIIGRM
                     NLTPGAAQMLRSLQGLHQMPMPPMGKVSGGGQSMSSVRMSDANGTIVVSSNSRTGTTV
                     HVTDSAGKVLYSGPYNTQEEKSAVPEAVRERLKNIETNFCF"
     gene            1771..>3127
                     /locus_tag="PK077_05550"
     CDS             1771..>3127
                     /locus_tag="PK077_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRN23800.1"
                     /translation="MNGFIFILWMLCCASWSLGADIFVSPDGDDAHAGTEEFPMRSVE
                     AAVRKAGQGHYGKGCQIILADGIYHLEEPLLLRPVHSGLTIRAKNPHKAVLSGAQPFV
                     ADWKPFRDGIMRTSVPSGLEMDVLLVNGKPMHMARYPNATPGERIFQGTAPDADAPER
                     VARWKNPAGAYLHALHHRMWGDMHFRITGKNGNTLVKQGGWQNNRPSPPHEKYKFVEN
                     VFEELDAPGEWYLDRKEHILYLYPEQGTDLAAARLEAGGLEELVKIQGEGSSPVRNVT
                     LRDLVLTGTRRTFMENREPLLRSDWTIFRSGAVLLRGTEGCRILSCEFARLGGTAVFV
                     DGNNENLLVRSCYIHDIGSNGVAFVGDRSCYRGPKNYREAKGMSLEGIDRVPGPRNNE
                     FPRNCMVDDCLITRTGLVEKQTAGVQISLAREITVRNCSIYELPRAGINIGEGAFGGH
                     VI"
     assembly_gap    3128..3137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3138..4130
                     /locus_tag="PK077_05555"
     CDS             <3138..4130
                     /locus_tag="PK077_05555"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRN23801.1"
                     /translation="DVFDTVRETSDHGSFNSWGRDRFWVRDQMALSQDKDVVLLDIRQ
                     PNIIRNNRWRCDHGWDVDLDDGSSNYIIYNNLMLSSGLKLREGFYRKVYNNIMVNKTL
                     YPHVWFRNSGDEFYNNIIFEDRYRPAGNMDFSPWGKLMDRNFVHVKGMKGVEPASELA
                     RQSGNDRHSLKGDALFSAPGLGDFSVRASSPALKLGFRNFPMDRFGVRSRHLKALART
                     PDIPEVAGNRLEKRETVLVKKLGAEVRIAEGEGDLSVFGLMPEDLGRALVIVKVQKDG
                     PCSSAGILPGDVLLMAGGNKVDGVEKLERLLPSSGKLTVTVRRNQENRKVDLQF"
     gene            complement(4168..5487)
                     /gene="dgt"
                     /locus_tag="PK077_05560"
     CDS             complement(4168..5487)
                     /gene="dgt"
                     /locus_tag="PK077_05560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HRN23802.1"
                     /translation="MMNWQQLLSDARWGSRHRITASPQKNRSNYDRDYGRILFSSAFR
                     RLQDKTQVFPLGRNDYVRTRLTHSLEVAHIGSSLGMLAGEWLAKKGSLPSPIMPSDVA
                     TIVSTACLAHDIGNPPFGHSGEDAIETALKRHGLELPFEGNAQGFRILTRTGDPMEGN
                     GLKLTAAVLGAFMKYPCTQSYSSAVKKGSIVAANKLECKKFGIGEQEREAASFMAGHL
                     GLIPRSTPDAAHLCWSRHPLAYLMEAADDICYRIADIEDGYFSGLLDFASTKDLFSPF
                     LTESQLCYVRELEAKEEKDSCIHYMRALAIGKSIQSAVDSFVNHEEDLLQGRLEQSLI
                     DSSELAAPLNGLYQYAIKNVYQAREVIEVEAMGYKVLGELIDFFMEWVNHPSSGQSQK
                     IAIMLQGTGVPRNNGGKAARLAHMLDYISGMTDSFALETYRKLTGIL"
     gene            complement(5556..>6325)
                     /locus_tag="PK077_05565"
     CDS             complement(5556..>6325)
                     /locus_tag="PK077_05565"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRN23803.1"
                     /translation="LVNRHGDFSPLKFTADIQPMDTQVIEFNAAEKTRGNWFFHCHIL
                     YHMMSGMGRIFTYEDSPPNPQLPHPMQALQHVYDMDRKWYLSVNNDFATNGNIGNLEF
                     GGTRWSMQGEWKIGYKDTRGYEAEGRLGRYIGEKQWLYPYIGADWTCRKGEAGERNMF
                     RQTTKKDREMDGTLGARYTLPLLLIADARIDTDGKVRLQLERDDIPLTSRLRLSFSLN
                     TDRDYSVGLHYILTSHLSVSTNYDKNLHWGVGLMLTY"
     assembly_gap    6326..6335
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6336..>6939)
                     /locus_tag="PK077_05570"
     CDS             complement(<6336..>6939)
                     /locus_tag="PK077_05570"
                     /inference="COORDINATES: protein motif:HMM:NF019351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HRN23804.1"
                     /translation="GKAFEFQATAEDTSGSSSLWLGHGERQPLRPFPKLNYFKKMKQM
                     NGMMAMGGNMKMHHHGMPTAHSGNMEIMDMKSGSSHGGGHGNMQEDGEETTLTYDMLK
                     SPSRTTLPSGVPVKELHFMLTGNMNRYVWSINGKTLSETDRIMIREGQNVRIILTNNT
                     MMRHPMHLHGHFFRLCLRRARPCCDPGCRTGQRQPGRLAD"
BASE COUNT         1558 a         1778 c         2009 g         1574 t
ORIGIN      
        1 aattttccgt ctgtaaaatg gcgcaagcca tgaattgcgc cagtgatgaa aaacgggaaa
       61 aagcgctgaa catccccctg tggcggcgtt gtgcctgtat ttccggcatt tccgcggctg
      121 ccgttgtggg ggccgtcatg ctggttcaac atgctctggt ggcgcccgta gggacggtga
      181 cagccggaaa tgccggggaa gttccctcgg aaaaggctga aactcctgtg attcccggtg
      241 aaaagcggga gttgctccgg acgcccaccc tttttgaatt gcagggaggg gatgagcgcg
      301 tgcctgtgat tgctcccgcc gttatccgca aaattcttcc tgaaaaggaa tattaaaaat
      361 tgcggtgcat gaatatcacc ggaattgtgc gatctgctgc ggcattgcgg ctttccttgg
      421 caggcaggaa agatgcctag cgttttccgg tgttctgcgg cgaaggatgc cgtcccggaa
      481 gcggcgaaaa aaatgcacaa attttgaaca ttgtatggac agatgctgta tattatctca
      541 catggatatg aaaacctgta tctttctgac attgggattg ctggtaggtt cgggctccgg
      601 atattccatt gaccggcccg caggaagtac ggacaacctt cagccgcctc ctctgacgcc
      661 gtacgcgcat caggtgaagc cgctcccctc ttccaaaccg gccagactgg gaattgttcc
      721 gggcacggtg ccccaagcgc tcgttgcgca gctggagctt agcggattcc ccggagtgct
      781 ggtgaccaaa gtgatgccgg acagccccgc cgccaaggcc gggctccagg aaaatgacgt
      841 catggtcaag ctgggggatg tctccctttc cggtccgcag tctgtgacgg aagccctgtc
      901 tgaaaaagtg cctggagaca ggattacggc tgtattttac cggaaaggaa agagggagac
      961 tgttgaaatt accctggatg ggggaacgct ttccgctgaa gaaatactgg cggcccaggg
     1021 ggatccccgc acgcagcccc gcgcagttcc ttccgtccgg cgtcagacgg cgcctttttc
     1081 cggaatggct gcacggccta atctccccca gcgtattctg gatatgcagc agatgatgga
     1141 tgagtttttg aaggattccg ccatggatga ttaccggatg gacgacatca tcggccggat
     1201 gaacctgact cccggcgcgg cgcagatgct ccggagcttg cagggacttc atcaaatgcc
     1261 catgcctccc atgggcaagg tttccggagg gggccagagc atgtcttccg tccggatgtc
     1321 ggatgccaac gggactatcg tggtttcttc caattcccgg acgggaacca cagttcatgt
     1381 gacggactct gcgggaaagg ttctgtattc cggcccctac aatacgcagg aggaaaaatc
     1441 cgccgtgccg gaagccgtca gggaacgctt gaaaaacata gaaaccaatt tctgctttta
     1501 acggcttcat gaaatagcgg agaaattcgt attcatgaag ttgacgacag ctgccccgtg
     1561 cggggcagct tttttattac gtggaagaag aggggaaagc tgtgttgatg cggctgatga
     1621 aaataatgac gggcagaatg aaaggaaaat catggaaatg cgtatccttt aacaaaatag
     1681 accgcttcat ggttaagtgc aggtgttggg aaaacggcgg ttttttcagg ctgcccgttt
     1741 tctggcggcg gtcttttcac aaagaacgac atgaatggtt ttatttttat tttgtggatg
     1801 ctttgctgcg cttcttggag cctgggagcg gatatatttg tttctcctga cggagatgac
     1861 gctcatgcgg ggacggaaga atttccgatg cgttccgtgg aggccgctgt gcggaaggcc
     1921 gggcaagggc attacggaaa aggctgccag attatcctgg ccgacggtat ttatcacctg
     1981 gaggaaccgc tgctcctgcg tccggttcat tccggtctga ctattcgtgc caagaatccg
     2041 cataaggcgg tattgagcgg tgctcagccc tttgttgcgg actggaagcc cttccgtgat
     2101 gggatcatga gaacttccgt gccctccgga ctggaaatgg acgtattatt ggtcaacggc
     2161 aaacccatgc acatggccag gtatcccaac gcaactcccg gagaacgcat tttccaggga
     2221 acggccccgg acgcggacgc tccggaacgg gtagcccgct ggaaaaatcc ggcgggtgcg
     2281 tatctgcatg cccttcatca tcgcatgtgg ggagacatgc acttccgcat tacggggaag
     2341 aatggaaata cactggtcaa gcaggggggc tggcagaata accgtccctc tccgccgcac
     2401 gaaaaatata aatttgtaga gaatgttttt gaggaactgg atgctcccgg agaatggtat
     2461 ctggacagaa aggaacatat cctttacctg tatccggaac agggaacgga cctggctgca
     2521 gccagactag aagccggcgg cctggaagaa ctggtcaaaa tccaggggga aggctcatcc
     2581 cccgtcagga acgtgacact ccgggatctg gtgctgactg gaacgcggcg aaccttcatg
     2641 gaaaaccggg agcccctgct gagaagtgac tggaccattt tcagaagcgg agccgtactc
     2701 ttgcggggaa cggaggggtg ccgcattctc tcctgcgaat ttgccaggtt gggtggaaca
     2761 gccgtttttg tggacgggaa taatgaaaat cttctggtgc ggtcctgtta tatccatgac
     2821 ataggttcca acggcgtagc gtttgtgggg gaccgttcct gctaccgggg accgaagaat
     2881 tatcgggaag ccaaaggaat gtccttggag ggaatcgaca gggttccggg acctcgaaac
     2941 aatgaatttc ccaggaactg catggtggat gattgcctga tcacccggac ggggctggtg
     3001 gaaaagcaga ctgccggagt gcagatatcc ctggcccggg aaattaccgt cagaaattgt
     3061 tccatctacg aacttccccg ggcgggtatc aacatagggg agggggcttt cggcggccat
     3121 gtgattgnnn nnnnnnngac gtttttgata ccgttaggga aaccagcgac catggttcgt
     3181 tcaactcctg gggacgggac agattctggg tccgggacca gatggcgctg agccaggaca
     3241 aggacgtcgt cctgctggat atccggcagc ctaatatcat caggaacaac cgctggcgct
     3301 gcgatcacgg atgggatgtg gacctggacg acggttcttc caactatatt atttacaaca
     3361 acctcatgct ttcctccggc ctgaagctcc gggaggggtt ttaccgcaag gtttacaaca
     3421 atattatggt taacaaaacc ctgtacccgc atgtatggtt caggaacagc ggggacgaat
     3481 tttacaataa cattattttt gaagaccgct accgtcctgc cggcaacatg gatttttccc
     3541 cgtggggaaa actgatggac aggaactttg tgcatgtgaa gggaatgaaa ggggtggaac
     3601 ccgcctccga actggccagg caatccggca atgacaggca ctccctgaag ggggatgccc
     3661 tgttctctgc tcccggtctg ggggattttt ccgtccgggc ttcttccccc gcccttaaac
     3721 tgggattccg caattttccc atggaccgtt tcggcgtgcg ttcccggcat ctgaaagccc
     3781 tggcgcgaac tccggatatt cctgaagttg ccgggaacag gttggagaaa cgggaaacgg
     3841 tgctggtgaa gaaactgggg gcggaagtga ggatagcgga gggcgaaggg gatttgtctg
     3901 tctttgggct gatgccggag gatctgggcc gggcgctggt tattgtcaag gtgcagaagg
     3961 acggcccttg ttcctctgcc ggcattctgc ccggcgacgt tcttctgatg gcgggcggga
     4021 ataaggtgga tggagtggaa aagctcgaac gcctgctgcc gtcttccggt aaattaaccg
     4081 taaccgtcag gagaaaccag gaaaatagaa aggtggattt gcagttttga ggatgcttct
     4141 ggcctccgga aaaagaggaa gcggaaatta cagaattccg gtcaacttcc ggtaagtttc
     4201 cagggcaaag gaatccgtca ttccggaaat ataatcaagc atgtgcgcca ggcgtgcagc
     4261 cttcccgccg ttgttccggg gtacgccggt accctgaagc atgatggcga ttttttggga
     4321 ttggccggag gatggatggt tcacccattc cataaagaag tcgatcaatt ctcccagtac
     4381 cttataaccc atggcttcca cttcaataac ctccctggcc tggtacacat tcttgatggc
     4441 gtactggtac aagccgttca gcggggcggc cagttccgag ctgtctatca gggattgttc
     4501 caaccttcct tgcagcaggt cttcctcgtg gttcacgaaa ctgtccacgg cggactgaat
     4561 gcttttgccg atggcgagcg cgcgcatata atggatgcag gaatcttttt cctcttttgc
     4621 ttccagttcc cggacataac agagctggga ttccgtcagg aaagggctga acaaatcctt
     4681 ggtggaagca aaatccagaa gtccggaaaa atacccgtcc tcaatatccg caatgcggta
     4741 acagatatca tccgcagctt ccatcaaata cgccaaggga tggcggctcc agcaaagatg
     4801 tgctgcatca ggtgtggaac gcggaatcaa ccccagatgt ccagccatga aagaagcagc
     4861 ttccctttcc tgttccccga tgccgaattt cttgcattca agcttgttgg ccgcaactat
     4921 gctgcctttc ttgacggcgg aggaataaga ttgcgtacag ggatatttca taaaggcgcc
     4981 caaaacagcc gctgtcagtt tcaggccgtt gccctccatc ggatctcctg tacgtgtcag
     5041 aatgcggaat ccctgggcat tcccttcaaa aggcagttcc aggccgtgcc gtttcagggc
     5101 ggtttcaatg gcgtcttcac cggaatggcc gaagggcgga ttgccgatgt catgggccag
     5161 gcaggcggtg gaaacaatgg tggcgacatc ggagggcatg ataggagagg gcagggaccc
     5221 tttcttcgcc agccactccc ctgccagcat gccgagggaa gaaccgatgt gggctacctc
     5281 caggctatgc gtcaggcgtg tgcgcacgta atcgttgcgg cccaggggaa aaacctgtgt
     5341 tttgtcctga aggcggcgga aagcactgga aaacagaatg cggccgtaat cccggtcata
     5401 attgctgcgg tttttctgtg gagacgccgt aatccgatgc ctggatcccc agcgcgcgtc
     5461 tgacaaaagc tgctgccaat tcatcatccc tgacatggta aggaaatgct acaggggaat
     5521 gcccggatgt aacaggagaa aaatagagag ggagttcaat aggtcagcat cagccccacg
     5581 ccccagtgca ggttcttgtc gtaattggtg gaaacgctga ggtgtgaggt caggatgtag
     5641 tgcaggccca cggaataatc ccggtccgta ttcagggaaa aggaaaggcg caggcgggag
     5701 gtgagcggga tatcgtcccg ctccaattgc aggcgcacct ttccgtccgt gtcaatgcgc
     5761 gcatccgcaa tcaggagcag gggcagcgtg taccgggcgc ccagggtgcc gtccatttcc
     5821 cggtcctttt tggtggtctg tcggaacatg ttgcgttctc cggcctcccc cttgcggcaa
     5881 gtccagtccg ccccgatgta ggggtaaagc cattgcttct ccccgatata cctgcccagc
     5941 ctcccttccg cctcataacc ccgcgtatcc ttgtatccta ttttccactc tccctgcatg
     6001 ctccaccggg tgcccccgaa ttccaggttc cctatgttcc cgttggtggc gaaatcattg
     6061 ttcacgctca ggtaccattt ccggtccatg tcgtaaacat gctgaagtgc ctgcatgggg
     6121 tggggaagct gaggattggg gggagaatcc tcatacgtaa aaatccggcc catgccgctc
     6181 atcatgtggt acaggatatg gcagtggaaa aaccagttgc cgcgcgtttt ctccgcagcg
     6241 ttaaactcaa tcacctgggt gtccatgggc tggatatccg ctgtaaactt gagcggagaa
     6301 aaatccccat gcctgttcac cagccnnnnn nnnnnctatc agccaggcgc ccaggctgac
     6361 gttgacctgt ccggcaacca ggatcacagc aaggcctggc acgacgaaga catagccgga
     6421 aaaaatgccc gtgcagatgc atgggatgcc tcatcatggt attgttggtc aggattatgc
     6481 gcacattctg cccttccctg atcatgatcc ggtccgtctc ggaaagagtt ttcccgttaa
     6541 tgctccatac gtaacggttc atattaccgg tcagcatgaa atgcaattcc ttaacgggca
     6601 caccggaagg aagagtggtg cgggaagggg acttcagcat gtcatacgtc agggtggttt
     6661 cttctccgtc ctcctgcatg ttgccgtgtc ctcccccatg ggaggaaccg gacttcatgt
     6721 ccattatctc catatttccg gaatgggcgg tcggcatgcc gtgatgatgc atcttcatat
     6781 ttcctcccat ggccatcatc ccgttcattt gcttcatctt tttgaagtaa ttcagcttgg
     6841 gaaaggggcg caaaggctgc ctttccccat ggcccagcca cagggaggaa gacccggaag
     6901 tatcctctgc cgttgcttgg aattcaaagg ccttgcctg
//
LOCUS       DAOMSU010000100         6922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_873_length_6922_cov_2.822484, whole genome shotgun sequence.
ACCESSION   DAOMSU010000100 DAOMSU010000000
VERSION     DAOMSU010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6922
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_873_length_6922_cov_2.822484"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PK077_05575"
     CDS             <1..502
                     /locus_tag="PK077_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23805.1"
                     /translation="AFHTEFRDTVSMRSAFFLLPAILPALSACGRGGATLETDNVQAE
                     WFPNGSRTAIVLEGESEPVSEREIRHPHSGMTLFYHSLGDGRGTLEIAGGVPETATVT
                     YSQGAASAELHFKTASREILLNDVRRLGANEKTAFLNGWSCQERGTALLRSGTAGAMT
                     TEKNVP"
     gene            499..>723
                     /locus_tag="PK077_05580"
     CDS             499..>723
                     /locus_tag="PK077_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23806.1"
                     /translation="MNLLRILKTGTWKIPCALLLAGVLALACLQCSVRSLVYLPPQPR
                     DKTAYLEKRAAWEPHRRTFHHNGAKLSGWLI"
     assembly_gap    724..733
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <734..1330
                     /locus_tag="PK077_05585"
     CDS             <734..1330
                     /locus_tag="PK077_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23807.1"
                     /translation="NAVGGFSSGRLYGGNAMDISMMLPYLDRFPHAKLLVNYRGYGLS
                     TGTPTEQDIMGDSLAILDSVLKETGRTPDDVILVGQSLGSGVATQVASVRHVKKLVLL
                     VPFDSLLETARGLFPYLPVRLLLPDHFRSDLAAPKVACPVSILAAGADEVIPPHHAKR
                     LRDCFSVPVSYQEFPGAMHNTIWLSPGFDKAFSQSIGY"
     gene            complement(1339..1749)
                     /gene="tsaE"
                     /locus_tag="PK077_05590"
     CDS             complement(1339..1749)
                     /gene="tsaE"
                     /locus_tag="PK077_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRN23808.1"
                     /translation="MTHSPEEMRELGRQIGKILMPGEILGVVGELGAGKTHLTQGIME
                     GLGSSEAAASPTFSLVHEHADGRLPACHFDFYRLKDESELTGIGWEEYLDGETVLIVE
                     WANLFPEALPEETSWLLLEHEGSCLRRVSLAPAE"
     gene            1877..2194
                     /gene="rplU"
                     /locus_tag="PK077_05595"
     CDS             1877..2194
                     /gene="rplU"
                     /locus_tag="PK077_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRN23809.1"
                     /translation="MAYAIFKTGGKQYRVQTGDVIDVECINTLEEGAEASFDQVLMVE
                     NDGKVTVGTPMVEGATVSAKVVSQFRGKKIIAFKFKRRKGFHKKKGSRRAMTKLEITA
                     INA"
     assembly_gap    2223..2232
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2233..2427
                     /locus_tag="PK077_05600"
     CDS             <2233..2427
                     /locus_tag="PK077_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRN23810.1"
                     /translation="KKFGGQEVIAGNIIIRQRGTKWHPGKGVGMGRDYTIFSLVDGRV
                     FFDREGRRVNVAPEGSEAAN"
     gene            2583..3023
                     /locus_tag="PK077_05605"
     CDS             2583..3023
                     /locus_tag="PK077_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HRN23811.1"
                     /translation="MNPKTDSKQAVRTASTLPVISGLRLTKQRQEVYQVLLEQRDHPT
                     ANEVYQRVRKKLPSISLATVYNCLEALVNHGLVNQVNFERSSSRFCPNLVEHGHFQDT
                     RTGRIYDITIKEGVDLKEFINLPDDVCVEEAQITLRGSITTPQG"
     gene            3039..3797
                     /gene="sufC"
                     /locus_tag="PK077_05610"
     CDS             3039..3797
                     /gene="sufC"
                     /locus_tag="PK077_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HRN23812.1"
                     /translation="MSLVIKNLHANVQGTEILKGINLEIPKGEVHAIMGPNGSGKSTL
                     SKVLCGHPDYEVTEGEAWLDGQNLLDMSIDERSRAGLFLAFQYPMEVPGVSNANFLRA
                     AMQARMPHGEELDAVTFYKTLYAKMDQLGMARKFTSRAVNEGFSGGEKKRNEVLQMLL
                     LDPLYAILDETDSGLDIDALRIVSEGVNSMRSPFRSFLVITHYKRLLDYIRPDVVHVL
                     ADGRIVRTGGGELVDELESRGYEFLKEAETAEKA"
     gene            3820..5256
                     /gene="sufB"
                     /locus_tag="PK077_05615"
     CDS             3820..5256
                     /gene="sufB"
                     /locus_tag="PK077_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HRN23813.1"
                     /translation="MSDTPDMPENGNTQNLFDFDRSKGNFSFPERHKFDAGYGLTEAT
                     IDYICDVKDDPEWVRQFRKNALAVFESKPMPTHWASPDIKKIDFSQIRYYLSDGERPK
                     RSWDDVPEDVKRTFERLGVPEQERQFLAGVEAQYDSESAYSNMKEELRRQGVIFVNST
                     EGLKYHEDVFRPWFGKVIPTGDNKFSALNSAVFSGGSFIYVPKGVKLKHPLQAYFRIN
                     SENFGQFERTLIIADEGAELMYMEGCTAPQFETSTLHSAVVELVALKGAKIQYVTVQN
                     WSSNVFNMVTKRGLAMEDAEIRWIDCNIGSDLTMKYPAVVLKGRRARGEVISIALANT
                     GQHQDTGAKMIHAADDTTSNIVSKSISIGEGRASYRGQVIMGKGLKGCKNNTECDALL
                     LAANSRTDTYPAITVKGDGGTVQHEASVSQVSEEMLFYMQQRGIPKAAAMSLAVNGFI
                     NDLVQEFPMEYSVELRRLIDLEMEDSIG"
     gene            5268..6521
                     /locus_tag="PK077_05620"
     CDS             5268..6521
                     /locus_tag="PK077_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HRN23814.1"
                     /translation="MNHLLNEEQFPAGFLPAWFEARRRHALEQAEQLPEPSRHMESWR
                     FGAPGNESLEHVEAAPPAAPETLAPIIRERASMPEALRIVYANGLPVSIPEELPEGLS
                     VMDMEDFVLQCPDTARKYLETETETLGSEKLSALATAFQHNGLVIMADREISLPLEIL
                     HFISGDNVAVFPSTLLIAETGSRLRILERHISADHGRQFCGALQQYHLSSASSVKYAL
                     VQELNSRSRAVELSHMMADDSAEMEHLVSHPGAAWARQETVCILNGDGAGVRLLSANH
                     LKENKELDQRTYQKHLYRGASSNLLYANVLDDEASSIFSGMILVAEGAHDTSAYQSNR
                     NLILSPRAEANSIPGLEILADRVQCSHGSATSSISPEEIFYLLSRGIPEQTARRMIAQ
                     GFLKHVLDQFGDETIRTAVEGIILP"
     gene            complement(6563..>6922)
                     /locus_tag="PK077_05625"
     CDS             complement(6563..>6922)
                     /locus_tag="PK077_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23815.1"
                     /translation="FVRTQGEESHFSPVVPDWRTNNKIHVTVPPGTKELSVLESYSRG
                     WSARTGKGEELPLSGTERYSILVSVPDSEQQAEILLQYHTPYRKWYYSMMGGTALFLL
                     VTALLQRRVNIRTEKEG"
BASE COUNT         1666 a         2073 c         1773 g         1390 t
ORIGIN      
        1 tgccttccat accgaattca gggacactgt ctccatgcga tcagcattct tcctactgcc
       61 cgccattctg ccggctcttt ccgcctgcgg gcgcggaggc gccacgcttg aaacggataa
      121 cgtccaggcg gaatggttcc ccaacggcag caggacggcg attgtcctgg aaggggaaag
      181 cgaacctgtt tccgaacggg aaatccggca tccccattcc ggcatgactc ttttttacca
      241 ttcgctgggg gacggcagag gaactttgga aatcgcagga ggggttccgg aaacagctac
      301 agtaacatac agccagggag cggcttccgc ggagctgcat ttcaaaacgg cctcccggga
      361 aatacttctg aatgacgtac gccgcctggg cgcgaacgaa aaaacggcct tcctgaatgg
      421 ctggagctgc caggaacggg gcacagccct gctccgttcc gggacagccg gagccatgac
      481 cacggaaaaa aacgtcccat gaatcttctg aggatactga aaacggggac atggaaaatc
      541 ccgtgcgcgc ttctgcttgc cggggttctg gctctggcat gcctccagtg ctccgtccgc
      601 tccctggtgt acctgccgcc ccagcccagg gacaaaaccg cgtatctgga aaaacgggcc
      661 gcctgggaac cgcatcggcg caccttccac cataatgggg ccaaactcag cggctggctt
      721 attnnnnnnn nnnaatgcag tgggaggatt ctcatcaggc cggctttacg gagggaacgc
      781 catggatatt tccatgatgc ttccctacct ggatcgtttt ccccatgcca agctcctggt
      841 caattaccgc ggatacgggc tcagcaccgg cacccccacg gaacaggata tcatgggaga
      901 ctccctggct attcttgact ccgtgctgaa ggaaaccgga agaacgccgg acgacgtcat
      961 cctggtcggg caaagcctcg gttccggagt cgctacccag gtagcctccg tcagacacgt
     1021 gaaaaaactg gtgctgctgg tcccttttga cagccttctg gaaactgccc gcggtctctt
     1081 cccatacctg cccgtcaggc ttctccttcc ggaccacttc cggtcagacc tggccgcgcc
     1141 caaagtcgcc tgccccgtca gcatcctggc cgcgggagcg gatgaagtca ttcctccgca
     1201 ccacgccaaa cggctccggg actgtttctc cgtaccggtc agttaccagg aattccccgg
     1261 agccatgcat aataccatct ggctcagccc cggctttgat aaagcgtttt ctcaaagcat
     1321 cgggtactga tctccgtttt attccgcagg agccagagac acgcggcgca ggcagcttcc
     1381 ttcatgttcc agcagcagcc atgaggtttc ttccggcagc gcttccggaa acaggttggc
     1441 ccattcgaca atgagcacag tctctccgtc cagatactct tcccacccta tgccggtcag
     1501 ctcggactca tccttcagcc ggtaaaaatc aaaatggcac gccggcaggc gtccgtcagc
     1561 atgctcatgc accagggaaa atgtcgggct ggcagccgcc tcggaactcc ccagcccctc
     1621 cataatgccc tgggtcaggt gcgtctttcc ggcccccagc tcacccacca cgcccaaaat
     1681 ttcgccgggc attaaaattt tacctatttg cctccccagc tccctcattt cctccgggga
     1741 atgagtcaaa acggggccgt ttctaaaaat tttatgccat tcgctcattt ttccatgaaa
     1801 ttttgcttgt ctaaaagaaa gtcatatggt ttattgcgac tcccgcatct gcgggcgaac
     1861 cttaacagtg atagcaatgg catacgcaat tttcaaaaca ggtggcaagc agtaccgcgt
     1921 ccagacgggc gacgtgatcg acgttgaatg catcaatacc ctcgaagagg gggccgaagc
     1981 aagcttcgat caggtgctta tggtggaaaa tgacggaaaa gtgaccgtcg gcacccccat
     2041 ggtggaagga gctacggtct ccgccaaggt ggtcagccag ttccgcggca aaaagatcat
     2101 cgctttcaaa tttaagcgcc gcaagggctt ccacaagaaa aaaggttccc gccgcgccat
     2161 gacgaagctg gaaatcactg ctatcaacgc ctaatttcaa cccttaattt ttattatctt
     2221 atnnnnnnnn nnaaaaaatt cggcggacag gaagtgatcg cgggcaacat catcatccgc
     2281 cagcgcggca ccaagtggca ccccggcaag ggcgtaggca tgggccgtga ctacaccatc
     2341 ttctccctgg tggatggccg cgtgttcttt gaccgcgaag gccgccgcgt caatgtggct
     2401 ccggaaggat ctgaagccgc caactaaaat acggattact ctatctccct gcaggggcgg
     2461 ccatccacag ggatgaccgc ccctttttct gtccttctcc gcgccccaat aaataacagg
     2521 aattgttctt gcaaaacaat tgcccctccc ccactatcca cgcagagcat gaaaaataag
     2581 atatgaatcc caaaacagat tccaagcagg ccgtccgcac cgcctctacc ctgccggtca
     2641 tttccgggtt gagacttacc aagcaaaggc aggaagtcta tcaggttctt ctggaacaaa
     2701 gggatcatcc aactgccaat gaagtttacc agcgggtacg caaaaaactc cccagcatct
     2761 ccctggccac cgtttataac tgcctggagg cgctggtgaa ccacggcctg gtcaatcagg
     2821 tcaactttga acgcagctct tcccgcttct gtcccaatct cgttgagcac ggtcacttcc
     2881 aggacacccg gaccggacgc atttacgaca tcaccatcaa ggaaggcgtg gacctgaagg
     2941 aattcatcaa cctgcccgac gacgtgtgcg tggaagaagc ccaaatcacc ctgaggggtt
     3001 ccatcaccac gccgcagggc taactctctt tcatcatcat gagtctggtc attaaaaatt
     3061 tgcacgccaa cgtgcaaggc acggaaatac ttaaaggcat caatctggaa attcccaagg
     3121 gagaggtgca tgcgatcatg gggcccaacg gctccggaaa aagcacttta tccaaagttc
     3181 tttgcggaca tccggactat gaggtaacgg agggggaagc ctggctggac ggacagaacc
     3241 tgctggacat gagcattgac gaacgcagca gggcgggact cttcctggca ttccagtacc
     3301 ccatggaagt tcccggcgtt tccaacgcca acttccttcg tgcggccatg caggcccgca
     3361 tgccccatgg ggaggaactg gatgcggtca ccttctacaa aacgctctat gcaaaaatgg
     3421 accagctcgg catggcccgc aaattcacgt cacgcgccgt taacgaaggt ttctccgggg
     3481 gagagaaaaa gcgcaatgaa gtgctgcaaa tgcttctcct ggatcccctc tatgccattc
     3541 tggacgaaac ggattccggc ctggacatag atgccctccg cattgtctcg gaaggagtca
     3601 actccatgcg ttcccctttc cgcagcttcc tggttatcac ccactacaaa cgcctgctgg
     3661 actacatcag gccggatgtg gtccacgtgc tggctgacgg gcggatcgtc cggaccggag
     3721 gcggagaact ggtggacgaa ctggaaagca ggggatacga gttcctgaaa gaagcggaaa
     3781 cagcggaaaa ggcgtaatac cgccatccgg ccatcaatca tgagcgatac acctgacatg
     3841 ccagagaacg ggaacacaca gaacctgttt gacttcgacc ggagcaaggg gaacttctcc
     3901 ttcccggagc gccataaatt tgatgccgga tacggcctga cggaggccac cattgactac
     3961 atctgcgacg tcaaggacga cccggaatgg gtgcgccaat tccgcaaaaa cgcgcttgcc
     4021 gtttttgaaa gcaagcccat gcccacccac tgggcttctc cggatattaa aaaaatagac
     4081 ttctcccaaa tccgctacta cctctccgac ggagaacggc ccaaacgaag ctgggacgac
     4141 gtgccggagg atgtaaagcg caccttcgaa cgcctgggcg ttccggaaca ggaacgccag
     4201 tttctggcgg gcgtggaagc ccagtacgac tccgaatccg cctactccaa catgaaggag
     4261 gaactccgca gacagggcgt catcttcgtc aactccacgg aaggcctgaa ataccatgaa
     4321 gatgtcttcc gtccctggtt tggaaaagtc attcccacgg gagacaacaa attctctgct
     4381 ctgaacagcg cagtattttc tggaggttcc ttcatttacg tacccaaagg ggttaaactc
     4441 aaacatcctc tccaggcgta tttccgaatc aactcggaaa atttcgggca gttcgaacga
     4501 actctcatca ttgcggatga aggtgcggaa ctcatgtaca tggaaggatg cacggcaccc
     4561 cagtttgaaa cttccaccct ccactccgcc gttgtggaac tggtcgcgct caagggcgcg
     4621 aaaattcagt acgtcaccgt gcaaaactgg tcctccaatg tcttcaacat ggtcaccaag
     4681 cgcggccttg ccatggaaga tgcggaaatc cgctggatag actgcaacat tggctccgac
     4741 ctcaccatga aatacccggc tgtggtgctc aaaggcagac gcgcgcgcgg ggaagtcatc
     4801 tccattgccc tggcaaacac gggccagcac caggatacgg gagccaaaat gatccatgcg
     4861 gcggacgata ctacgtccaa catcgtttcc aaatccatca gcatcggcga agggcgcgcc
     4921 agctaccggg gccaggtaat catgggcaaa ggactcaagg gttgcaaaaa caacacggaa
     4981 tgcgacgccc ttcttctggc ggccaacagc cgtacggaca cctaccccgc catcacggta
     5041 aaaggggatg gaggcacggt gcagcatgag gcatccgtct cccaggtctc cgaagaaatg
     5101 cttttttaca tgcagcagcg gggcattccg aaagctgcgg cgatgtccct ggccgtcaat
     5161 ggattcatta acgacctcgt tcaggaattc cccatggaat actctgtgga gctgcgcagg
     5221 ctcattgatc tggaaatgga agacagcatc ggataaggag ggaaacaatg aaccatttgc
     5281 ttaacgaaga acaattcccc gcaggctttc ttcccgcatg gtttgaagcc cggcggcgcc
     5341 atgccctgga gcaggcggaa cagcttccgg aaccttcccg ccatatggaa tcctggcgtt
     5401 tcggagcccc cggcaacgaa tccctggaac atgttgaagc ggctcctcct gcagctccgg
     5461 aaaccttggc tcccatcatc agggaacgag cctccatgcc ggaggccctc cgcattgttt
     5521 acgccaacgg gctccccgtt tccatcccgg aagaactgcc ggaagggctt tccgtaatgg
     5581 atatggaaga ctttgtgctg caatgcccgg acaccgcccg gaaatacctg gaaacagaga
     5641 cggaaacgct cggttccgaa aaactgtcgg cattggctac ggccttccag cacaacgggc
     5701 tggttattat ggcggacagg gaaatctccc tccctctgga aatactccac ttcatctccg
     5761 gagataacgt ggccgttttt ccctccacgc tgctcattgc ggaaaccgga agccgcctgc
     5821 gcattctgga acgccacatt agcgcagacc atggccgcca attctgcgga gcgctccagc
     5881 agtaccatct ttcctccgcg tcatctgtca aatacgcact ggttcaggaa cttaacagcc
     5941 gttcccgtgc cgtggagctt tcccatatga tggcggatga ttccgcggaa atggaacatc
     6001 tcgtcagcca tcccggcgca gcatgggcca ggcaggaaac ggtctgcatc ctgaacggag
     6061 acggcgcagg agtccgcctg ctttccgcca accatctgaa ggaaaacaag gagctggacc
     6121 agagaaccta ccagaagcac ctttaccgcg gagcctccag caacctgctt tacgccaacg
     6181 tacttgacga cgaagccagc agcattttca gcggcatgat cctggtggcg gaaggcgcgc
     6241 atgacaccag cgcctatcaa agcaaccgca atctcattct cagccccaga gcggaagcca
     6301 actccattcc cggcctggaa atcctggcgg acagagtgca gtgttcgcac ggttccgcca
     6361 cttcctccat ttccccggaa gaaattttct acctgctttc acggggcatt ccggaacaga
     6421 cggcgcgcag aatgattgcc cagggctttc tgaaacatgt cttggaccaa ttcggcgatg
     6481 aaaccatacg taccgcggta gaaggcatca tcctgccctg accgctattt catgaaaccc
     6541 aaaaagacgg agagacggtt ccctatccct ccttttccgt cctgatatta acgcgcctct
     6601 gcaagagagc cgtcacaagc agaaacaggg ccgttccccc catcatgctg taataccatt
     6661 tacggtaagg cgtatggtac tgaaggagga tttccgcctg ctgttccgaa tccggaacgg
     6721 aaacaagaat agaatagcgc tcagtgccgg ataaaggaag ctcttccccc ttcccggtac
     6781 gggcgctcca tcccctggaa tagctctcaa gaacagacaa ttcctttgtt cctggcggca
     6841 cggtgacatg aatcttatta ttcgttctcc aatccggaac aacaggagaa aaatgactct
     6901 cttccccctg ggttcggacg aa
//
LOCUS       DAOMSU010000101         6862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_884_length_6862_cov_2.748494, whole genome shotgun sequence.
ACCESSION   DAOMSU010000101 DAOMSU010000000
VERSION     DAOMSU010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6862
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_884_length_6862_cov_2.748494"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="PK077_05630"
     CDS             <1..804
                     /locus_tag="PK077_05630"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRN23816.1"
                     /translation="CVFDSLAACIFGPRQGAGNLSFFFHLPGRGLRQLAAALEKRHPG
                     LHASVLPCDLADETSLNTLISRLDSLPPGRTLLINNAGAGDYGDFADGRWEKIRSLLR
                     LNVESLTRLCHALIPVMKRNGGDIINLSSLGALLPIPDFAVYAATKAYVSSLSEALRL
                     ELREHGIRVMAVCPGPVSTGFGKAARRPGFTGNMMPGRNAFDTSVETVVKDSLRALAR
                     GRARVFPGMKIRLAAMLLAAAPLGLIRLFMGRRPRKVLPASNDSPSSAS"
     gene            801..2372
                     /locus_tag="PK077_05635"
     CDS             801..2372
                     /locus_tag="PK077_05635"
                     /inference="COORDINATES: protein motif:HMM:NF018983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 75 protein"
                     /protein_id="HRN23817.1"
                     /translation="MKAHRRGIPRLTWFKLAVCLCLAAMAVSLFTPYPAQIIRALKGE
                     PEQAPPDSGTCPPQESPPAEQPGQEEQPITAVPSASGKEPGDERLYTPWTPERTIALP
                     PVQYPPFPPVLPTRPAEGAMTHIYELARGLNLKTQVNFQPGTLASRDRETKSNYQMTL
                     SLNVKQPKALTKKEDILKLNPKLEPMLPGLSTLFRHARVSPYYGQVYVRKQTEIRKNL
                     ASLLKLLDRHNYYDTETILETAYPDTGRKLLWLQSEMDVVSDGSDGDRLAAMPDKILK
                     SSFYQPSTSYRWKKRTDKPNPLLNPWQQRLASYKKTLEKAPAAEKTALRRKIDHAERV
                     IEELKRYSFLISEYDPFIVVPLGVVNQSSPFSPQFGDYAVVIVGDKLYPALVGDAGPR
                     YKTGEGSLRLSREINPKAGPYSRPVSDLKVSYLIFPGSAEPEAGPPDYEKLTDRCREL
                     LNEIGGMGKGFKLHQWEDLLAPKPPPAPKPESGGTAETSADKRKKEEAPGNKPAPAPA
                     SPQTAPASSAAKSAA"
     gene            complement(2374..3273)
                     /locus_tag="PK077_05640"
     CDS             complement(2374..3273)
                     /locus_tag="PK077_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HRN23818.1"
                     /translation="MKGLDHLLSAVSRSFYLSMRFLPREMRSGVAVGYLLARATDTVA
                     DAVDMDCRERLALLRMMGETVAGSASREVAATCFGRLGVLSTIQTHRGEAELLARFPE
                     CLALLENLSPHERMLVRQVLASIVEGQAWDLMYFESHAAVSCPEQLEHYTYMVAGCVG
                     EFWTRLGLLAQGPGFSTQPEEQLLRWGRHYGMGLQLVNILRDREEDLARGRSYLPAPD
                     DRPWLDRAERWLKEGVFYAESLRNGRLRFSTVLPAWLGLDTLELLQRADRRHPSSGKV
                     KIARGDVYFRMWKAFLFSWKEAL"
     gene            complement(3270..4763)
                     /locus_tag="PK077_05645"
     CDS             complement(3270..4763)
                     /locus_tag="PK077_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRN23819.1"
                     /translation="MVVLKEISSAIVNERNGTALLRHILDVLYRRMKMLRGTFTIRRG
                     NDLMIEASHGLDEQEMKRGHYHVGEGITGHVAETGRPHVIEDISRDSRFLNRTRTRKS
                     GEKVAFICVPIIHLQQVIGTLSIDRAVDRDTDLERDQKLLEIVGNIVGDALAASLQAH
                     EEHAALVAENEKLRELLTTNPGELIGNCSTMLQVYEQIRQVAPSDATVLIRGSSGTGK
                     ELVARAVVNLSGRKDKPLVTLNCAAMPENLLESELFGHEKGSFTGATSRRIGRAEAAD
                     GGTLFLDEIGDLSLQMQVKLLRFLQEKTFSRVGSNRELHADVRFIAATSRNLEELMAA
                     NKFREDLYYRLNIFPIVMPDLSKRKGDVMLLAEHFLSKFNLKYGKDIKRLSTPAINML
                     MAYHWPGNVRELENCMERAVITAQDDCIYGYNLPASLQMPSHDVPYSRDGEAPADLPT
                     MVDSFERELIVAALKRSPGNMSAAARELGISPRVLHYKMHRLGLQKS"
     gene            4732..4968
                     /locus_tag="PK077_05650"
     CDS             4732..4968
                     /locus_tag="PK077_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23820.1"
                     /translation="MAELISFSTTISKSGDLFISFMQSGAGRILTARPRHTNNFFFHR
                     HAAGQKNGTGNMDNIPAINVDDITNGEQKIFTKM"
     gene            complement(4994..6781)
                     /locus_tag="PK077_05655"
     CDS             complement(4994..6781)
                     /locus_tag="PK077_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRN23821.1"
                     /translation="MNSPSPQSQPPDSVWYGWILKLFHHWKVGERQVTYVWAVLVGMV
                     GSCMSLFFEWMVEVIQWMLTGIWSDSRVGTFSLVEPEWRMFIPVAGGLVAGCILLFLK
                     KRMPGQATEYMEAMSIGNGLIKFRASALKVFSAAWSIASGAAIGKEGPIIQSSALIAS
                     TVGQRLHVSIPRLRLLVGCGAAAGFTTAFHAPFSGCLFVSEIIIGTVSMDILAPLLIA
                     SCTGFVMLHLLGDPTPLYSSPFESFTVFSQSLWCIALGAAAAVAARGWVCLLDAAARY
                     LNGRQSLLPLRLAAAGLVVGILAVFYPEVVGNGQALITGLVHEDYGTREALVLLLVKV
                     SAVAVVFGCGTVGGAMTPTLYVGSMVGFIFSTVLTSLGMEGNHTVAYAMVGMASFFAV
                     AAQAPLTALVMVVEFSMSGQMIYPLLIGVVSAYGTGKLIRARSMYAASLAGSPASVVS
                     LPMAQVHVHDLARHVVPQVAPDASLDDVSSLMLRNPGDLVFVVNKDGTYEGAIYPEDF
                     LAVRRQMEERKGAAPADAGSLVRTGAPVLDAGTHLTDALKLFEQTHLPAFPVVWKNTG
                     RLDGVLYRNALFQVITEMMKRESGGDVGM"
BASE COUNT         1563 a         2236 c         1820 g         1243 t
ORIGIN      
        1 tgtgtttttg acagccttgc cgcctgcatc tttggccccc gacagggtgc gggaaatctc
       61 tcttttttct ttcaccttcc gggccgtggc ctgcgccagc ttgccgccgc cctggaaaaa
      121 cgccatcccg gccttcacgc ctccgtcctt ccctgcgacc tggcggacga aacctccctg
      181 aatacgctca tttcacgcct ggacagcctt cctccgggaa gaacccttct gattaacaac
      241 gccggagccg gtgattacgg agattttgcg gacggccgct gggaaaaaat acgttccttg
      301 ctgcgtctga acgtggaaag cctcacccgc ctgtgccatg cgctgatccc cgtcatgaag
      361 aggaacggag gggatatcat caacctcagt tccctgggag ccctcctgcc cattccggat
      421 tttgccgttt atgcggccac caaggcttat gtttccagcc tgtcggaagc gctgcggctg
      481 gaactgcgcg aacacggcat ccgcgtgatg gccgtctgcc ccggcccggt ttccacaggc
      541 ttcgggaaag cggcgcgccg ccccggcttc accggaaaca tgatgccggg gcgcaatgct
      601 tttgacacgt ccgttgaaac cgtcgtgaaa gacagcctcc gcgccctggc ccgcggacga
      661 gcccgggtct ttcccggcat gaaaatacgg ctggccgcca tgctgctggc tgccgccccg
      721 ctggggctga tacgcctttt catgggcaga cgtccccgga aagtcctgcc ggcatccaat
      781 gattctcctt cttcagcatc atgaaagcgc accgccgcgg aataccccgc ctcacctggt
      841 tcaagctcgc agtctgcctc tgcctggcag ccatggctgt ctccctgttc acgccgtacc
      901 ccgcccagat catacgcgcc ctgaaagggg agccggaaca ggcgccgccg gattccggaa
      961 cctgtccgcc ccaggaatcc ccccccgcgg aacagcccgg gcaggaagaa cagccaataa
     1021 ccgctgtgcc atctgcctcc ggaaaggaac ccggggatga acggctgtac actccatgga
     1081 cgccggagcg cacaatcgcc cttcccccgg tgcagtaccc cccatttcct cccgtgcttc
     1141 ccacaaggcc cgcggaaggg gccatgaccc atatttatga actggcccgc ggcctgaacc
     1201 tgaaaacgca ggttaatttc caacccggca ccctggcctc cagggacagg gaaaccaaaa
     1261 gcaattacca gatgaccctt tccctgaacg tcaaacagcc caaagcattg acgaaaaagg
     1321 aagacattct gaagctcaat ccgaaactgg aacccatgct tcccggcctg tccaccctgt
     1381 tcaggcatgc gcgcgtctcc ccttattacg ggcaggttta cgtccgcaaa cagacggaaa
     1441 tccgtaaaaa cctggcctcc ctgctcaaac ttctggaccg ccataattat tacgatacgg
     1501 agaccattct ggaaaccgcc tatccggaca cgggaaggaa gcttctctgg ctgcaaagtg
     1561 aaatggatgt tgtttccgac ggctcggacg gggaccggct ggccgccatg ccggataaaa
     1621 tcctgaaaag ctccttctat cagcccagca cttcctaccg ctggaaaaag cgcacagaca
     1681 agcccaatcc cctgctcaac ccctggcagc agaggctggc ctcctacaag aagacgctgg
     1741 aaaaagcccc cgccgcggaa aaaacggctc tgcgccgcaa aatagaccat gcggaacggg
     1801 tcattgagga actgaagcgg tacagcttcc tgatctcgga atacgatccg ttcatcgtgg
     1861 ttccgctggg agtcgtcaac cagtcctctc ccttctcccc gcagttcggg gactatgccg
     1921 tcgtcatcgt gggagacaag ttgtacccgg ccctggtggg agacgccggg ccgcgctaca
     1981 aaactgggga ggggtccctg cgcctgagcc gtgaaatcaa ccccaaggcg ggcccttaca
     2041 gccgccccgt ctccgacctg aaggtaagtt acctgatttt tcccggttcc gcagagcctg
     2101 aggccggccc acccgactat gaaaaactta cggacagatg ccgggaactt ctcaacgaaa
     2161 taggcggcat ggggaaagga ttcaaactcc accaatggga agacctgctg gcgcccaagc
     2221 cgccgcccgc cccaaaaccg gaaagcggcg gcacagcgga aacatccgcg gataagcgga
     2281 aaaaggagga agcccccggg aacaagcccg cgccggcccc ggcttccccg caaactgcac
     2341 cggcttcatc agccgcaaaa agcgccgcat aagctacaga gcctccttcc aggaaaaaag
     2401 aaaggctttc cacatccgga aataaacgtc tccgcgcgca atcttcacct tcccggaaga
     2461 cggatgccgc cgatccgccc gctggagcaa ttccagcgtg tccagcccca gccaggcggg
     2521 caggacagtg gaaaaacgca gccgcccgtt ccgcagagat tccgcgtaaa acaccccttc
     2581 cttcagccag cgttccgcac ggtccagcca tggacggtca tctggggcgg gcaagtagct
     2641 tcttccgcgg gcgaggtctt cctcccggtc acgcagaata ttcaccaact gcagccccat
     2701 gccgtagtgc ctgccccagc gcaaaagctg ctcctccggc tgcgtgctga agcccggccc
     2761 ctgagccagc aaacccagcc tggtccagaa ttcccctacg cagccggcca ccatgtaagt
     2821 ataatgttcc agctgttccg gacatgagac ggcagcatgg ctttcaaaat acatcaaatc
     2881 ccacgcctgt ccctccacga tggaggccag cacctgccgc accagcatcc gttcatgcgg
     2941 ggaaaggttt tccagaagcg ccaggcattc gggaaaacgg gccagcaatt ccgcctcccc
     3001 ccggtgcgtc tggattgtgg aaagaacgcc cagacggcca aaacaggtgg cggcaacctc
     3061 ccgggaggca gagccggcca ccgtctcccc catcatcctc aaaagcgcca ggcgctcccg
     3121 gcaatccata tccacggcat ccgccaccgt atccgtggca cgcgccagca gatagcccac
     3181 ggccacgccg gagcgcattt cccggggcag gaagcgcatg ctcagataaa aggaccggga
     3241 gacggccgat aaaagatggt caaggccttt catgatttct gcaggcccag cctgtgcatc
     3301 ttataatgga gtactcgcgg ggaaatgccc agttcccggg ccgcggcgga catattgccc
     3361 ggagaacgtt tcaatgcggc tacaatcaat tcccgttcaa aggaatctac catggtcggc
     3421 aaatcggcag gagcctcccc gtcacgggaa taagggacgt catggctggg catctgaagg
     3481 gaggcgggaa ggttgtaacc gtaaatgcag tcgtcctgcg ccgtaatgac ggcacgttcc
     3541 atgcagtttt ccagctcccg gacgttgccg ggccagtggt aagccatgag catgttgatg
     3601 gcgggggtgg acagccgttt gatgtccttt ccgtatttca ggttgaactt ggaaagaaaa
     3661 tgttccgcca gcagcatgac gtcccccttg cgtttggaga ggtccggcat aacgatgggg
     3721 aaaatgttca accggtaata caagtcttcc cggaacttgt tcgccgccat caattcctcc
     3781 agattgcggc tggtggcggc gatgaagcgc acgtctgcat gaagctccct gttgcttccc
     3841 acgcgggaaa aggtcttttc ctgaagaaag cgcagcagct tcacctgcat ctgaaggctt
     3901 aaatccccga tttcatccag aaacagggtg ccgccgtccg ccgcctccgc ccggccgatg
     3961 cggcggctgg tggccccggt aaaagaaccc ttttcatgac cgaacagctc gctttccagc
     4021 aggttctccg gcatggccgc acaattcagc gtgaccagcg gcttatcctt tctgccgctc
     4081 agattcacca cggcgcgcgc caccagttcc ttccccgttc cggaacttcc ccgtatcagc
     4141 accgtggcat cgctgggcgc cacctggcgg atctgttcat acacctggag catagtgctg
     4201 caattgccaa tcaattcgcc cggattagtc gtcaaaagct cccgcagttt ttcattttcc
     4261 gcaaccaggg cggcatgttc ctcatgcgcc tggaggctgg cagccagagc atccccaaca
     4321 atattgccta caatctccaa cagtttctga tcccgttcca ggtcggtgtc ccggtccacg
     4381 gcacggtcaa tgctcaacgt gccgatgacc tgctgcaaat gaatgatggg aacgcagatg
     4441 aaggctactt tttcaccgct tttgcgcgtg cgagtacggt tcaggaacct ggaatcccgg
     4501 gaaatatcct caatcacatg gggcctgccc gtttccgcca catgccccgt aatgccttcc
     4561 cccacatggt aatggccgcg tttcatttcc tgctcgtcca ggccgtggga ggcttcaatc
     4621 atcaaatcgt tcccgcggcg gatggtgaac gtgccgcgca gcattttcat gcggcggtaa
     4681 agcacgtcca gaatatgccg gagcaaagcc gtgccgttgc gttcattgac aatggcggag
     4741 ctgatttctt tcagcacgac catctccaaa tccggtgacc tgtttatttc attcatgcaa
     4801 tccggggctg ggcgaatatt aaccgcacgc ccccgccata ccaacaactt tttcttccac
     4861 cgccatgcag ccgggcaaaa aaacggtacg gggaatatgg ataacatccc agcaattaat
     4921 gttgatgata ttacgaacgg ggaacaaaag atttttacaa aaatgtaata tacatccctt
     4981 gtttcgttcc gttttacatg cccacgtctc cgccggactc ccgtttcatc atctccgtga
     5041 taacctggaa caacgcgttg cggtacagca cgccgtccag ccgaccggta ttcttccaga
     5101 ccacgggaaa ggctggcaga tgggtttgtt caaacagctt caaggcatcc gtcagatggg
     5161 tgccggcgtc cagcacggga gcgcccgtac ggaccaggga cccggcatcg gccggggccg
     5221 cccccttccg ctcctccatc tgccggcgaa ccgccagaaa gtcctccgga tagatggccc
     5281 cttcataagt tccatcctta ttcaccacaa aaaccagatc tccgggattc cgcagcatca
     5341 gggaagagac atcatccagg gaagcatccg gggccacctg gggcaccacg tgccgcgcca
     5401 ggtcatgcac atggacttgc gccatgggca gactgacgac ggacgccgga ctgccggcca
     5461 ggctggcggc atacatggaa cgcgcgcgaa tcagttttcc cgtgccataa gcgcttacga
     5521 caccgatcag caagggataa atcatctgtc cgctcatgga aaactccacc accatcacca
     5581 gagccgtcag gggggcctgg gcagctacgg cgaaaaagga cgccataccc accatcgcat
     5641 acgccaccgt atggttgccc tccattccca gggacgtcag aaccgtactg aagataaaac
     5701 ccaccatgct ccccacgtac agggtgggcg tcatggctcc ccccaccgtg ccgcagccga
     5761 acaccaccgc cacggcagac accttcacca gaagcaggac gagcgcctcc cgcgtgccgt
     5821 aatcctcatg gaccagcccg gtaatgagag cctgaccgtt gcccaccact tccggataaa
     5881 aaacggccag aatccccacg accagcccgg cggccgccaa tcgcagcggc agaagggact
     5941 ggcggccatt caaatatctg gcggccgcat ccagcaaaca gacccatccc ctggccgcca
     6001 cggccgccgc cgcccccagg gcgatgcacc agagagactg ggaaaagaca gtaaagcttt
     6061 caaacgggga actgtacaaa ggggtgggat cccccagcag atgcagcatc acgaacccgg
     6121 tacaggaggc gataagcagg ggggccagaa tatccatgct cacggtgccg atgataattt
     6181 cactcacgaa caggcagccg gaaaaagggg catggaacgc cgtcgtgaat ccggctgccg
     6241 ctccgcagcc gaccagcaac cggagccgcg gaatggatac atgcagacgc tgccccacgg
     6301 tagacgcaat cagggcggaa gactggatga taggcccttc cttgccgatg gccgcgccgg
     6361 aagcgatgct ccatgccgcg gaaaaaactt tcagggcgga ggcccggaac ttgatcagcc
     6421 cgttcccgat ggacatggcc tccatatatt ccgtagcctg ccccggcatc ctcttcttca
     6481 ggaatagcag aatgcagccg gccaccaggc cgcccgccac cggaataaac atgcgccatt
     6541 ccggctccac cagggaaaag gttcctacgc gggaatccga ccagatgccg gtgagcatcc
     6601 actggatcac ttccaccatc cactcgaaaa acagagacat gcaggagccc accatgccga
     6661 cgagaacggc ccatacataa gtaacctgcc tttcccccac cttccaatga tggaacagtt
     6721 ttaaaatcca tccgtaccag acggaatccg gcggctggga ctggggggac ggagaattca
     6781 tggaaaagaa ggcaatacta ttccattgtg acaaaaaaat acctccggct gttcgatacg
     6841 ggagaaaaaa ctgcccagcc cc
//
LOCUS       DAOMSU010000102         6782 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_904_length_6782_cov_2.893861, whole genome shotgun sequence.
ACCESSION   DAOMSU010000102 DAOMSU010000000
VERSION     DAOMSU010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6782)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_904_length_6782_cov_2.893861"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PK077_05660"
     CDS             <1..844
                     /locus_tag="PK077_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23822.1"
                     /translation="EYIYETPHFRFICDAKLGTGMIKRLGLLFEATHLANKTLPIGNS
                     PAHDDSAKFPAYLYEKFSTYLENGGREGTAGIFLGTTRPGDRGRILVPFDSLGVKTMG
                     STYVIDRDKDASTLIHELTHQLMSSQAKQASWFCEGSAEYMGMTPYAGGRFNFGANRS
                     HIVSRVTEYGKKNTGGRALGDDFEAPGLEAYMNMPYSQFTGENANLNYGLAALMAYYF
                     YHMDGKGDARRIKNYMKAIQSGTSEKEAQKLLLDGRSYEELAKEIEQKWRKAGVKIRF
                     RSSS"
     gene            972..>1658
                     /locus_tag="PK077_05665"
     CDS             972..>1658
                     /locus_tag="PK077_05665"
                     /inference="COORDINATES: protein motif:HMM:NF037626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23823.1"
                     /translation="MAEEASISALMDRVARTAPFHRELAKLGTEEQAVFDHTSLPGVP
                     FVAALCIRTLPDTARAWVVAPHLRAQEALAAQLETWGVRHLLFIPEKEVALGEEMGDP
                     ELAAERLNILHRISSSRTGGQTIILTEGSLNDEVPSPDGMQNQGITLKTGETHAPDDL
                     IRQFEEAGFEGVPQVISRGQWSRRGGILDVFPLQSSHPVRLEFFDDEIESIREFDVDS
                     QISFRKAEHVN"
     assembly_gap    1659..1713
                     /estimated_length=55
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1714..4164
                     /gene="mfd"
                     /locus_tag="PK077_05670"
     CDS             <1714..4164
                     /gene="mfd"
                     /locus_tag="PK077_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRN23824.1"
                     /translation="DWIKPDDLVITAPFCKERGNVCILTSPPENAAGEEDFSLAIHDN
                     PLGSFEAGDFVMQEMRRELAERQIREWLDRKWNVSMFFPNEGEEERFRDICAGTPALL
                     SITPLRGELPAGFSIPEAKTAVLSSSELFGRYQSATARRRASREDKARKARAQASLKD
                     VNPGDLVVHTSYGIGKFINISASPDSGDEEMNILYRDNTILHVPLSQAHLVSRYIGLG
                     SKTPELNKLGDSKWQRAKKSAERSVADYAAQLLNVQAERQTGKGYSHPPDSKWMWEFE
                     SSFPFRETQDQLRAIAQTKADMESTRPMDRLICGDVGFGKTEVAIRAAFKCVTGGRQA
                     AVLVPTTVLAEQHFRTFKERMSEYPVRIEMLSRFSSAADIRSTLEGLRTGAVDIVIGT
                     HRLISDDVSMKNLGLVVIDEEQRFGVRHKEKFKERFKGIDVLTLSATPIPRTLYIALM
                     GARDMSSIETPPVNRQPVQTSVCPYDERIMKKAMERELERGGQIFLLHNRVKTIELFR
                     DRIQALVPKAHIVIGHGQMPKEELEKVMRTFVQGKADILLATTIIESGIDIPNANTII
                     IDRADRFGLADLYQLRGRVGRAGHRAYAYLMLPRSAATTGDARKRVSAIKQYTELGSG
                     FKIAMRDLEIRGAGNLLGTQQSGHIAAIGFDLYCQLLQQSIAQMQGKRTAPRPDAALR
                     TDFIINSETQFAAKSRKDYLGAFLPREYISDAALRISAYKDLAAVRTLKEADNLLRAW
                     EDRFGPAPETVRHLLDSHKIKILASHANISMVEISGQRLMLTRNGDFILLSNKFPRLA
                     SAAPADKLRETLDMLKNI"
     gene            complement(4312..4509)
                     /locus_tag="PK077_05675"
     CDS             complement(4312..4509)
                     /locus_tag="PK077_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23825.1"
                     /translation="MSRSFRSALTQESPVLEVSMNMDDYAENVENFEWSIEVIMAPAN
                     TCGIMEMMRKKELSNGKEKQI"
     gene            complement(4564..4809)
                     /locus_tag="PK077_05680"
     CDS             complement(4564..4809)
                     /locus_tag="PK077_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23826.1"
                     /translation="MLGILYSQGWGVDYSAEMVMRLSKLLAGSGYDLSWLTLSAPILA
                     HWELLGQQEGTYTRMCCSIPDRKMNQFSGVRLVRTSK"
     gene            complement(5050..5769)
                     /locus_tag="PK077_05685"
     CDS             complement(5050..5769)
                     /locus_tag="PK077_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRN23827.1"
                     /translation="MFSRSPFLCGDSLSLMECMDNDCYHAVVTDPPYASGGLTTAERK
                     ASPARKYLSSSRYLSFDNDTRDQSTHFLWSVMWMQEAIRITRHGGWLMVFSDWRQLPL
                     TSDALQVAGWTWRAVVTWDKTEACRPHQGMFRNQSEFILIATRGSIGKEQDRPRVFPA
                     GVFRHYLKPSDKMHLTGKPVPLMRHLMTVLPPGSRVLDPFMGSGTTLVAARDLGHVAH
                     GIDLSPDNVRIAADLLGVPVP"
     gene            complement(5834..6199)
                     /locus_tag="PK077_05690"
     CDS             complement(5834..6199)
                     /locus_tag="PK077_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23828.1"
                     /translation="MTKAKQLILPISYPDSGRWYAPVRKIKIDERTYALRMGEPVQLG
                     TVKEIEKEFRVGRRVLARLADIGVIERVRPSPFQSMYYFADIAAFLERTRTAPAFWSS
                     VRREAYLNGHSPQDASLRL"
     gene            complement(6202..>6506)
                     /locus_tag="PK077_05695"
     CDS             complement(6202..>6506)
                     /locus_tag="PK077_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23829.1"
                     /translation="VIMILTRSELAKMTELFDAQELPDTFPDDLEFSFLEVLAPTMTL
                     QDYLAMPEQERRKWWINAVPDSVFPLDVPDQETGEPLFMTGFYFEPVCPGILDRVY"
     assembly_gap    6507..6516
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6517..>6782
                     /locus_tag="PK077_05700"
     CDS             <6517..>6782
                     /locus_tag="PK077_05700"
                     /inference="COORDINATES: protein motif:HMM:NF022039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 97 catalytic
                     domain-containing protein"
                     /protein_id="HRN23830.1"
                     /translation="APWIKPGRSTWTWWDRGNVLENDQYAFVDMTAEFGWEYHLVDEG
                     WKKWGRSLPESMDKLAKLASYAAGKNVGIWVWVRWSDVNNPAND"
BASE COUNT         1613 a         2015 c         1867 g         1222 t
ORIGIN      
        1 agaatatatt tatgaaacac cccatttccg cttcatctgc gacgccaagc tgggcaccgg
       61 catgatcaag cgcctgggcc tcctctttga ggccacccac ttggccaaca aaacccttcc
      121 tataggaaac tcccctgccc atgacgattc cgccaaattc cccgcctacc tgtatgaaaa
      181 attcagcacc tatctggaaa acggcggacg cgaaggcacg gcgggcatct tcctgggaac
      241 aacgcgtccc ggggaccgcg gaagaattct ggttccgttc gattccctgg gagtcaaaac
      301 catgggaagc acatacgtca ttgaccgtga caaggacgct tccaccctca tccatgaact
      361 gacgcaccag ctcatgtctt cgcaggccaa gcaggccagc tggttttgtg aaggctccgc
      421 ggaatacatg ggcatgacgc cctatgccgg tggccgcttc aactttggag ccaaccgatc
      481 ccacattgtc tcccgcgtga cggaatacgg caaaaaaaat acggggggac gggcccttgg
      541 ggatgacttt gaggcgcccg gcctggaagc ttacatgaac atgccctatt cccagttcac
      601 gggagaaaac gccaacctga actacggcct ggccgccctg atggcctact atttttacca
      661 catggacggc aagggcgatg cccggcgcat caagaattac atgaaagcca ttcaatccgg
      721 aaccagtgaa aaggaagctc agaaactcct ccttgacgga cggagctatg aagaactggc
      781 caaagaaatt gaacagaaat ggcgcaaggc cggcgttaaa atccgcttcc gttcctcttc
      841 ctgaactatg ccgcggcgtg ccgtggaaac agtcccccga cggaaaacga agggcggttt
      901 gtgccctgaa aaaccgttga cacgggaggg aaaggcggca cacttccctc aacagggatt
      961 cacagacacg catggcagaa gaagcttcca tatccgccct gatggacagg gtagcccgta
     1021 ccgccccctt tcaccgggaa ctggcaaaac tgggcacaga ggaacaggcg gttttcgacc
     1081 atacttccct gcccggggtg ccgtttgtag cggccctgtg tatcagaacc cttccggata
     1141 ccgcacgggc ctgggtggtc gcgccccatt tgcgggctca ggaagccctt gcagcccaac
     1201 tggaaacctg gggagtgcgc cacctcctct tcattccgga aaaagaggtg gccctggggg
     1261 aggaaatggg ggacccggag ctcgccgcgg aacgtctgaa tatccttcac cgcatctctt
     1321 ccagccgcac gggcgggcaa accatcattt taacggaagg cagcctgaac gatgaggttc
     1381 cgtcccccga cggcatgcaa aaccagggga tcaccctgaa aacgggggag acgcacgcac
     1441 cggacgactt gatacggcag tttgaggaag cgggatttga aggtgttcca caggtcattt
     1501 cccgcgggca atggtcccga cgcggcggca tcctggatgt ctttcctctc cagtcctccc
     1561 accccgtgcg cctggagttt tttgacgatg aaattgaatc catccgggaa tttgatgtgg
     1621 attcccagat ttccttccgc aaggcggaac acgtcaacnn nnnnnnnnnn nnnnnnnnnn
     1681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngactgga tcaaaccgga cgacctggtt
     1741 attaccgccc ctttctgcaa ggaacggggg aacgtatgca tcctcacctc ccccccggaa
     1801 aatgcggcgg gggaagaaga cttctccctt gccatccatg acaatcccct gggcagcttt
     1861 gaggcggggg acttcgtgat gcaggaaatg cgccgtgaac tggcggaacg ccaaatccgg
     1921 gaatggctgg accggaaatg gaatgtcagc atgttcttcc ccaatgaagg ggaggaagaa
     1981 cgcttccggg acatctgcgc cggaacgccc gctctgctgt ccatcacccc tcttcgcgga
     2041 gaacttccgg cagggttcag catcccggaa gcgaaaacgg ccgtcctctc ttcctcggaa
     2101 ctgttcggca ggtaccagtc cgctacggcg cgccgccgcg ccagccggga agacaaggcg
     2161 cgcaaggcac gcgcgcaggc atccctgaaa gacgtcaatc ccggtgatct ggtggtgcat
     2221 accagctacg gcatcggcaa attcatcaac atttccgcct cccctgactc cggagacgag
     2281 gaaatgaaca tcctctaccg ggacaacacg atcctgcacg tccccctcag ccaggctcac
     2341 ttggtttcac gatacatagg cctgggaagc aaaacgccgg aactcaacaa actgggggac
     2401 tccaaatggc agcgcgccaa aaaatccgca gaacgatccg tggcggatta cgccgcccag
     2461 ctcctcaatg tccaggcgga acgccagaca ggaaaaggct acagccaccc cccggacagc
     2521 aaatggatgt gggaatttga aagctccttt cccttccggg aaacccagga ccagctccgc
     2581 gccatcgccc agacgaaagc ggacatggaa tccacccgcc ccatggaccg cctgatctgc
     2641 ggagacgtgg gcttcggaaa aacggaagtt gccatccgcg ccgccttcaa atgcgtgacg
     2701 ggaggccgcc aggcggccgt gctggtcccc accaccgtat tggcggaaca gcatttccgc
     2761 acgttcaagg aacgcatgag cgaatacccc gtacgtattg aaatgctcag ccgcttcagc
     2821 agcgcggcgg acatccgctc cacgctggaa gggctcagaa cgggagctgt ggacatcgtc
     2881 ataggcacgc accggctcat ttccgatgac gtatccatga aaaatctggg cctggtagtc
     2941 attgacgagg aacagcgctt cggcgtcagg cacaaggaaa aattcaagga acgcttcaag
     3001 ggaattgacg tgctgacgct gtccgccacg cccattcccc gcaccctgta cattgccctg
     3061 atgggagcac gtgacatgag ctccattgaa accccgcccg tcaaccggca gcccgtccag
     3121 acaagcgtgt gcccttacga cgaacgcatc atgaaaaagg ccatggaacg cgagctggag
     3181 cgcggaggac agattttcct gctgcacaac cgcgtgaaaa ccatcgaact gttccgggac
     3241 cgcatccagg ccctggtgcc caaggcccac atcgtcatcg gccacggcca aatgcctaaa
     3301 gaagaactgg aaaaggtcat gcgcaccttc gtgcagggga aagcggacat cctgctggcc
     3361 accaccatca tcgaatccgg catcgacatt ccgaacgcca acaccatcat cattgaccgc
     3421 gcggaccgct tcggactggc ggacctttac cagctccgcg gccgcgtggg ccgtgcgggc
     3481 caccgggcct acgcctacct catgctcccc agatccgccg ccaccacggg agacgcccgc
     3541 aaacgcgtct ccgccatcaa acagtatacg gaactgggtt ccggcttcaa aattgccatg
     3601 agggacctgg aaatacgcgg tgcgggcaac ctgctgggaa cccagcagag cggccacatc
     3661 gccgccatag gctttgacct gtattgccag cttctccagc aatccatcgc ccagatgcag
     3721 ggcaaacgca cggccccccg tccggatgcg gctctaagaa cggactttat cattaacagc
     3781 gaaacgcagt tcgccgcaaa atcccgcaag gactatctgg gagccttcct tcccagggaa
     3841 tacattagtg acgcggccct gcgcatctcc gcctacaagg atctggccgc cgtccggacc
     3901 cttaaggaag cggacaatct gctccgcgcc tgggaagacc gcttcggccc cgcgccggaa
     3961 acggtgcgcc acctgctgga ctcccacaaa atcaaaatcc tggcatctca tgccaacatc
     4021 tccatggtgg aaatcagcgg gcagcgcctg atgctcacca gaaacgggga ttttatcctg
     4081 ttgtccaaca aattcccgcg gctggcaagc gccgctcccg cggacaaact ccgggaaacg
     4141 ctggacatgc tgaaaaacat ttaaatccgc tgcggtcatc tcgtggctga attcacccat
     4201 gggaaaacat gaaaacccgg aaaacagttc ttgccccttt ccatgccggg aacggggttg
     4261 cggcaggaat acgaaaccgc gtatccgcca gaccgctccg gcagctttcc atcaaatctg
     4321 cttctcttta ccgttggaaa gctccttttt ccgcatcatt tccatgatgc cgcatgtgtt
     4381 ggccggagcc atgatcactt ctattgacca ctcgaaattc tctacattct ctgcgtaatc
     4441 atccatattc atggacacct ccagaacagg agattcctgg gtaagagccg aacggaaaga
     4501 acgggacata acttcctgcc catccctgag caggatcacc ttgtcaataa agagacttcc
     4561 cccctatttg gaagtccgca ccagacgcac gcctgaaaat tggttcatct tcctgtccgg
     4621 aatggaacag cacatccgcg tataagttcc ctcctgctga cccagaagct cccaatgggc
     4681 taaaatgggg gcggacaaag tgagccacga caaatcataa ccggaaccgg ccaacagttt
     4741 ggacagcctc atgaccatct ctgcggaata atccaccccc cacccctggg aatacaaaat
     4801 gcccagcatt gtgaccaacc ataaatgctt gaaaagggtt gaattcgctt gaagaagctt
     4861 gaaaacggtt gaaaatacgt gcgttccggg gagaaatgga agggtggcgc aagaagttac
     4921 gcccataaat gcccgtaagg gtcaattttg aaaaatcgta agggtcattt gtcccatgtg
     4981 agacactccc gccagcggtc cgcgcgtgtc tcgcggaagc ccggcgaact ctccgcgggc
     5041 tggccgcggt cagggaacag gaacgcccag gaggtccgcg gcaatgcgaa cattatccgg
     5101 ggaaaggtcg atgccgtgag cgacgtgtcc caggtcgcgg gcggcaacaa gagtcgtccc
     5161 gctccccatg aaagggtcga ggacgcggga accgggagga agaaccgtca tcaggtggcg
     5221 catgagcgga acaggcttgc ctgtgaggtg catcttgtcg gaaggcttga gataatgccg
     5281 gaaaacgcca gccggaaaca cgcgcgggcg gtcctgctcc ttgccgatgc tgccgcgcgt
     5341 ggcaatgagg ataaactccg attggttgcg gaacatgccc tggtgtgggc ggcaagcctc
     5401 cgtcttgtcc caggtgacga cggcgcgcca tgtccacccg gccacctgca aggcgtccga
     5461 ggtcaaagga agctgcctcc agtcggaaaa gaccatcagc cagccgccgt gccgggtgat
     5521 ccttattgcc tcctgcatcc acatgacgga ccacaaaaaa tgggtgctct ggtccctggt
     5581 gtcgttatca aaagaaagat accttgagga agacaggtat ttccgggcgg gggaagcctt
     5641 gcgttccgcc gtcgtcaggc cgccggaagc gtacggcggg tccgtaacga cggcgtgata
     5701 acaatcgttg tccatgcact ccatcaggga aagggagtcg ccgcacagga aaggggaacg
     5761 tgaaaacatg ctcgcatgat ggcacaaaac aggccgcggc tttcacgccg cggccaggat
     5821 aaaaaatgtt agattacaac cggagggagg catcctgcgg agaatggccg ttcagataag
     5881 cctcccggcg gacggaggac cagaacgccg gggccgtccg cgtccgctca aggaatgcgg
     5941 caatgtccgc aaagtagtac atagattgaa aaggagacgg gcgcacccgc tcaatgacgc
     6001 caatgtccgc caggcgggcc agcacgcggc ggcccacccg gaattctttt tcaatctctt
     6061 tgacggtacc cagctggaca ggttccccca tccttagggc gtacgtccgt tcgtcgattt
     6121 tgattttccg gacgggagca taccagcgtc cgctgtccgg gtaggaaata ggaaggatga
     6181 gttgctttgc tttggtcatg gttaatagac gcggtcgagg attccggggc atacaggttc
     6241 aaagtaaaac ccggtcataa agaggggttc ccccgtttcc tggtccggga cgtccagggg
     6301 aaaaacgctg tcagggacgg cattgatcca ccatttccgc cgctcctgtt ccggcatggc
     6361 gagatagtcc tgcagggtca ttgtcggagc caggacttcc aggaaggaaa attccaggtc
     6421 gtccgggaac gtgtcaggca attcctgcgc gtcaaaaagt tcagtcattt tggcaagttc
     6481 ggaacgggtt aaaatcatga taactgnnnn nnnnnnagcc ccctggatca agccgggccg
     6541 ttccacctgg acctggtggg accgcgggaa tgtcctggaa aacgaccaat atgcctttgt
     6601 cgatatgact gccgaattcg gatgggaata tcacctcgtt gacgaagggt ggaaaaaatg
     6661 gggccgatca cttccggaaa gcatggacaa gctggccaaa ctggccagct acgccgccgg
     6721 caaaaacgta ggcatctggg tatgggtgcg atggtcagac gtcaacaacc ccgccaatga
     6781 ct
//
LOCUS       DAOMSU010000103         6759 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_911_length_6759_cov_2.768496, whole genome shotgun sequence.
ACCESSION   DAOMSU010000103 DAOMSU010000000
VERSION     DAOMSU010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6759
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_911_length_6759_cov_2.768496"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            377..712
                     /locus_tag="PK077_05705"
     CDS             377..712
                     /locus_tag="PK077_05705"
                     /inference="COORDINATES: protein motif:HMM:NF013089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HRN23831.1"
                     /translation="MSWIYLVLAGLCEIGWPLGFKLSQTPGTGPGKFAACIACSVFSM
                     VLSGFLLWLAQRNIPIGTAYAVWTGIGALGTFLVGILWFGDSSNIWRMLAASFLLIGI
                     IGLKLAPGH"
     gene            complement(780..2714)
                     /locus_tag="PK077_05710"
     CDS             complement(780..2714)
                     /locus_tag="PK077_05710"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HRN23832.1"
                     /translation="MFGYYRLASSVPQLRVAEVDYNVDQLTEEFRKAAEQQAAAVVFP
                     ELCITGYSCGDLFFQPNLRKAALNGLLRFTEATEGSGTIAVVGLPFLHEDALYNTAAV
                     VQSGRILALVPKTVLPNYREFYEKRQFTSGRELGTGVKEVTVNGMHIPFGTEIVFHEE
                     SSPFSFGVEICEDLWSVIPPSSKLALLGARAILNPSAGTELTGKAAYRRELVRQQSGR
                     CLCAYVLSSAGVHESTTDTVFGGHSLIADNGRPAAEGERFCRESTLIFADVDFERLEA
                     ARLSESSFNDSKSLFPAGNALHLALPEQVPGAPGLEYAFNPARPFLPSPSRRRERCEE
                     IISIQTAGLAKRMEHTRAQRLVIGISGGLDSTLALLICSRACRALKRPASDILAVTMP
                     GFGTTDRTHDNAVTMCRLLGVELREIPISECCLRHFADIGHDPAERTTTYENVQARER
                     TQILMDLANKTGGLVVGTGDLSEIALGWSTYNGDHMSMYAVNCSIPKTLIRCLIEHIA
                     EESSPELAATLTDINNTPVSPELLPPSDDGTIEQKTEDVLGPYDLHDFFLFHFIKYGA
                     EPDKILHLAEHAFRGEFQPDFIRRCLGIFIRRFFRQQFKRSCMPDGPKVGTISLSPRG
                     DWRMPSDACGTVWEKAISHY"
     gene            3033..3782
                     /locus_tag="PK077_05715"
     CDS             3033..3782
                     /locus_tag="PK077_05715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRN23833.1"
                     /translation="MNNTDRFTGKARAYAQGRPDYPASVVGLLTRESRRENPRLADIG
                     SGTGILSRAMLERGWTVYGVEPNDDMRKEAEKRLSAFPRFHSVAGTAERTGLPGASVD
                     LVTAAQSFHWFDTAAFKRECRRILFGGGKVALIWNSRVEDSPVVREEGNIHRLYCPCF
                     YGFSGGLEKLTDSIGAFFNHRFQVFRFPNDLSYTREQYLRRMMSTSYALTEGGEERSS
                     WLGALEKLFDRFETEGRVTVPNETVVYLGKC"
     gene            complement(3833..4228)
                     /locus_tag="PK077_05720"
     CDS             complement(3833..4228)
                     /locus_tag="PK077_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23834.1"
                     /translation="MSLHSAPSIVWELSGEPCETRDQALSGIKNNTEAFIICSRGDEF
                     LQAICNSPDNYHYEISFAGGKDRELYMTPKDLDYSRLHDLFSRYAAGEDLSHLKKEWT
                     LDVHKPGYITAIVVVLAIIAVLAVIIFSS"
     gene            4570..5349
                     /locus_tag="PK077_05725"
     CDS             4570..5349
                     /locus_tag="PK077_05725"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HRN23835.1"
                     /translation="MMHQTFSQEPFLVSGLHGDQILGARSNQQDSFSILAGEDILVLV
                     LADGMGGYTGGELASRAAVEGCLRAFERKSGLPGERMKAAVTAANEQVWSARKKRGED
                     DEMGTTLVAAWIGEDGLRWVSVGDSLLLLIREEKMFLLNELHQYSAELERMADEGIIS
                     REEALSHPARHSLTSALMGKEVPLVDLREECFPLFPGDRVLVASDGVGPYLDSLGPAG
                     MRYLSMLSAEELVRSVLDGISRDGDPNQDNATLICVEIASA"
     gene            complement(5494..>6759)
                     /locus_tag="PK077_05730"
     CDS             complement(5494..>6759)
                     /locus_tag="PK077_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRN23836.1"
                     /translation="AQALKDAGVKIMGTQPEGIRLAEDREYFRERMIALNIRQPESGT
                     ARSLEEAVELGRRIGYPVMVRPSFVLGGRGMEVIYDEENLKRYGVEAIQVSPEYPMLI
                     DRFLDNAIEAEVDALADGTDTFVATVMEHIELAGIHSGDSACAIPPVTIAPKHIRTIE
                     EHAAKIAQDFQVKGILNVQFAICNDEVYIIEANPRASRTVPIVSKVTGISLARYATEI
                     MLGKKLKELGLKSRACRFIGVKEAVFPFNMFPEVDPVLGPEMRATGEVMGIADNFGMA
                     YYKAQEAAGCILPTAGKVLVTVSDRDKKFIEPIARDLISLGFTIVSTGGTAEYLRGQG
                     VETEVVNKLHEGRPNLGDMITNKQIDLIINTPVDRTSMIDDSFIRMQSIQKKIPYMTT
                     IAAARATVEGIRSAQHVKVSPRSLQEYHS"
BASE COUNT         1381 a         1710 c         2088 g         1580 t
ORIGIN      
        1 gattcccgtg gaagccatgc ttccggtcag aatgatggcg gcaagtgaat aatgaatagc
       61 cataacggaa ccaatatcac agggcggagg ctcccggctc aaggatattc aagaaggctt
      121 gaaataagac cgttttttaa ttaaaaagaa caggtatatg gcttatggta aatatgtttt
      181 gtgtagactc catgtttgaa agaaccattt tcccaaagcc ggtgaaacag ggtgtatccc
      241 atttcatacg ggttcataaa aaattattgc cttgatcgga aacgccgggc agatattctg
      301 cgaagcatcc gttgcccttg gagcttttcc catttgggaa ggccggtgag cggaaggtga
      361 ttccccgttt caatatatgt cctggattta tctggtgttg gccggtctct gcgagatcgg
      421 atggcctctg ggtttcaaac tttctcaaac gcccggcaca gggcccggca aattcgccgc
      481 ctgcatcgcc tgctccgttt tcagcatggt tctgagcggt tttcttttgt ggcttgccca
      541 gcggaacatc cccatcggca ccgcttatgc ggtgtggacc ggcattggcg cgttgggcac
      601 gttcctggtt ggtattctgt ggttcgggga ttcttccaat atctggcgca tgctggcggc
      661 atccttcctg ctgataggca ttatcgggct gaagttagcc cccgggcatt gatgtggtct
      721 ttggaatcct tgccgggttc cgggaaaagc cgtctccgca gctttccgga taggaggtct
      781 cagtagtgtg agatggcttt ttcccacacg gtcccgcagg cgtcggaagg catgcgccag
      841 tccccgcgcg gggaaaggga aatggtgccc accttggggc cgtccggcat gcagctgcgc
      901 ttgaattgct gacggaagaa acggcggatg aagatgccga ggcagcggcg gatgaagtcc
      961 ggctgaaatt cgccgcggaa ggcatgttcc gccaggtgca ggattttatc cggttccgca
     1021 ccgtatttaa tgaagtggaa gaggaaaaag tcgtgcaggt cgtaaggccc aagcacatct
     1081 tccgttttct gctcaatggt gccgtcgtcc gacggcggca ggagttccgg gctgacggga
     1141 gtgttgttga tgtccgtgag ggtggctgcc agttccgggg atgattcttc cgcgatgtgt
     1201 tcaatgaggc agcgtatcag cgttttcgga atggagcagt tgaccgcgta catggacatg
     1261 tggtccccat tgtaggtgct ccatcccaag gcaatttccg acaagtcccc tgttcccacg
     1321 accagccccc cggttttatt ggccaaatcc atcagaattt gcgtgcgttc gcgggcctgg
     1381 acgttttcat aagtggtggt tcgttcggca ggatcgtgcc cgatgtcggc gaagtgccgg
     1441 aggcagcatt ccgagatggg gatttcccga agttccacgc ccagaaggcg gcacatggtt
     1501 acggcattgt catgggttct gtccgtggtg ccgaaaccgg gcattgtcac ggccagaatg
     1561 tccgaggccg ggcgcttcag cgcacggcag gcgcgggagc agatgagcag agccagcgtg
     1621 gagtccagcc cgccggagat gccgatgacg agccgttgcg cgcgggtatg ttccattctt
     1681 ttggccagac ctgccgtttg gatggagatg atttcctcac accgttcccg gcggcgggac
     1741 ggagaaggca gaaagggccg tgccgggttg aaagcgtatt ccaggccggg cgctccggga
     1801 acctgttccg gcagggccag atgcagggca ttcccggcag ggaaaaggga tttgctgtca
     1861 ttgaaggagc tttcagagag gcgcgccgcc tccagccgtt caaaatccac gtccgcaaag
     1921 atgagggtgc tttccctgca gaaccgttcc ccctccgctg ctggcctgcc gttgtccgca
     1981 atgagggaat ggccgccgaa tacggtgtcc gtggtggatt catgcacacc tgcggaggaa
     2041 aggacatagg cacagaggca tctgccgctc tgctgtctga ccagttccct cctgtatgcg
     2101 gccttaccgg tgagttccgt tcctgcggaa gggttgagaa tggctctggc tcccagaaga
     2161 gccagtttgg aactgggagg aatgacgctc cacaggtctt cgcatatttc cacgccaaaa
     2221 ctgaaggggg aagattcttc atgaaaaacg atttctgtcc cgaacgggat atgcatccca
     2281 ttgactgtga cttccttcac ccctgtaccc aattccctgc cggaggtgaa ttgacgtttt
     2341 tcatagaatt cccggtagtt ggggagcacg gttttgggca ccagagccag aatgcggccg
     2401 gactgcacta ccgccgccgt attatacaga gcgtcttcat gaaggaaggg caggcccacc
     2461 acggcaatcg tgccggagcc ttccgtggct tccgtaaagc ggagcaggcc gttcagggct
     2521 gctttcctca ggttcggctg gaagaaaagg tctccgcagg aatagcccgt aatgcacagt
     2581 tccgggaaaa cgacggctgc cgcctgctgt tctgcggctt tccggaattc ctccgtaagt
     2641 tggtcgacat tgtagtccac ttcagccacg cgaagctggg ggacggagga ggcaagtctg
     2701 taatatccga acatgggcgg agcagtctga aaaatggaac ccgcagatgt tcctttttcc
     2761 ggtagtcagg acgcaatccg ggaaaggcgg gcctgagccg gatactaaaa gttcatgaaa
     2821 gacttgtaaa gccatctgta gggactgctc cgcccatgtt gccggggaag gggcaaggaa
     2881 cgcaattttt cccgcatgcc tttctgcatg acggtcaccg ggcaaatcag ttttttcccg
     2941 ggatcccgca gcatggcggc ttttcttgag ccgggtacgg cattgaggct ggaatagcgg
     3001 gagaaaagga gtaaaccata ccctgaaaac taatgaataa tactgaccga tttactggaa
     3061 aagcccgggc ttatgcccag ggcaggcccg attatcccgc ctccgttgtt gggcttctga
     3121 cgcgggagag ccgccgggag aatccccgtc tggcggatat aggttccgga acgggcattc
     3181 tttcccgcgc catgctggag cgcggctgga ccgtgtatgg cgtggagccc aatgatgaca
     3241 tgcggaagga agcggagaag aggctgtccg cttttccccg ttttcattcc gtggcgggaa
     3301 cggcggagcg gaccgggctt cccggagctt cggtggatct ggtgacggcg gcgcagtcat
     3361 tccactggtt tgacaccgcc gcgttcaaga gggagtgccg ccgtattctg ttcggaggag
     3421 gcaaggtagc cctgatttgg aactccaggg tggaagacag ccccgttgtc cgggaggaag
     3481 gaaatatcca ccgtctttac tgtccgtgtt tttatggatt cagcggaggg ctggagaagt
     3541 tgacggacag cattggagcg tttttcaacc accgtttcca ggtattccgc tttccaaatg
     3601 atttgtccta cacgcgggag cagtatctcc gccgcatgat gtccacctcc tatgccttga
     3661 cggagggcgg ggaagagcgg tcctcctggc tgggcgccct ggaaaagctg tttgaccggt
     3721 ttgaaacgga ggggcgcgtt acggttccga atgaaactgt ggtgtatctg gggaagtgct
     3781 gaggatgcgg cgttcatgca tggactccgg aaaaggaaaa gcctctttcg gttcaggaac
     3841 tgaagatgat gacggcaagg acggcaatga ttgccagaac gacaacgatg gcggtaatat
     3901 agccgggttt gtggacatca agagtccact cctttttgag atgggaaaga tcctcccctg
     3961 ccgcatagcg tgaaaaaagg tcatggagcc gggagtagtc caggtctttg ggcgtcatgt
     4021 aaagctccct gtcctttccg ccggcaaacg aaatttcgta atggtaatta tccggactat
     4081 tgcagatggc ctggaggaat tcgtcaccac ggctgcagat aatgaaagct tccgtgttat
     4141 ttttaatgcc ggacagggct tgatcccgcg tttcacaggg ctctccggaa agttcccaaa
     4201 cgatggaggg ggcggaatga aggctcatgg aataaaaaaa tcggaaaata tggcggagaa
     4261 ttatttttcc tgccgccagt aaaaactatc ctttcttctt cctgggcgga aggtgtttcc
     4321 tggctcaata ctacggagaa tactgggagc gcttgcgggt tggggcattc aatggctgtt
     4381 tttccgctta tcattccggt tttgcatggg gtccttccgt cggggtgttt ccttcgcatt
     4441 ttcccggcca tgcaggctga cgttttcgtt cccctggaat aatcattcca cggcttcagg
     4501 catggctatt ttctctcgct attcttgctt tattaaggca ggccatccat gttacgatta
     4561 tttactgcca tgatgcatca gactttttcc caagagcctt ttctggtttc cggattgcat
     4621 ggggaccaga ttctgggcgc ccgcagtaac caacaggatt ctttctccat tctggctgga
     4681 gaggacatat tggtactggt gctggcggac ggcatgggtg ggtataccgg cggagaattg
     4741 gccagccgcg cggcggtgga aggttgtttg cgggcttttg agcggaaatc cggtttgccc
     4801 ggagagagaa tgaaagctgc agtgaccgcc gcgaatgaac aggtatggtc cgcccggaag
     4861 aaaagagggg aggatgacga gatggggaca acgctggtag cggcctggat aggggaggat
     4921 gggctgaggt gggtcagcgt gggggattcc cttctgctgc ttatccggga agaaaaaatg
     4981 tttctgctta atgagctgca ccagtattcc gcggaattgg agcgcatggc ggatgaaggg
     5041 atcatcagcc gggaggaggc tttgagccat cctgcccgcc attccctgac ttctgccctg
     5101 atggggaagg aagttccttt ggtggatttg cgggaggaat gctttccctt atttccgggg
     5161 gaccgtgttt tggtcgccag tgacggagtg gggccttatc tggattccct ggggccggca
     5221 gggatgcggt atttgtccat gctttctgcg gaggaactgg tgcgttccgt actggacggc
     5281 atcagccgtg acggggaccc caaccaggat aatgccacac tgatttgtgt ggagatagca
     5341 agtgcttgat tcttaagtag tacggcaagg agatgatctg cggacagtcg attttttgag
     5401 attcaaatcc tcccgcctgc cggatgatga acaaaacccc gcatgcgcct caggcacgcg
     5461 gggttttgtt taaacagcag gagagtttcc cgttcaggaa tggtattcct ggagggaacg
     5521 gggtgatacc ttcacgtgct gggcggagcg gatgccctcc accgtggctc tggcggctgc
     5581 gatggtagtc atgtacggaa ttttcttctg aatggactgc atgcggatga aggagtcgtc
     5641 aatcatactg gtgcggtcca ctggagtgtt gataatcagg tcgatttgct tgttggtgat
     5701 catgtcgccc agattcgggc ggccttcatg cagcttgttg actacttccg tttccacgcc
     5761 ctggccgcgc aggtattctg cggtgccgcc ggtggagaca atggtaaagc ccagggaaat
     5821 caggtcccgg gcgatgggtt cgatgaactt cttatcccgg tcggaaaccg tgacgagcac
     5881 cttgccggcg gtaggcagaa tgcagcccgc agcttcctgg gccttgtaat aggccatgcc
     5941 gaagttgtcc gcaatgccca tgacttcacc cgtggcgcgc atttccggcc ccagtacggg
     6001 gtccacttcc gggaacatgt tgaaggggaa gacggcttcc ttcacgccga tgaaacggca
     6061 ggcgcgcgat ttcaggccca gctccttgag tttcttgccc agcatgattt ccgtggcgta
     6121 gcgcgccagg gagattcccg ttaccttgga gacgataggc accgtgcggg aagcgcgggg
     6181 attggcttca atgatgtaaa cctcatcgtt gcagatggcg aactgcacgt tcagaatacc
     6241 ctttacctga aagtcctggg caattttggc ggcatgttcc tcaatggtgc ggatgtgctt
     6301 gggtgcgatt gtcacggggg ggatggcgca ggcggagtcg ccggaatgaa tgccggccag
     6361 ctcaatgtgt tccatgaccg tggctacaaa agtatccgtg ccatcggcca gcgcatccac
     6421 ttccgcctca atggcattgt ccagaaaacg atcgatcagc atgggatatt ccgggctgac
     6481 ctgaatggcc tccacgccgt agcgcttcag gttttcttca tcgtaaatga cttccatgcc
     6541 gcggccgccc agcacgaagg aggggcgcac catgaccggg tagccgatgc ggcgccccag
     6601 ttccacggct tcctccaggg aacgggccgt gccgctttcc ggctggcgga tattcagggc
     6661 gatcatacgt tcacggaaat attcgcggtc ttccgccaga cggatgcctt ccggctgggt
     6721 tcccatgatt ttcacgccgg cgtccttgag ggcctgggc
//
LOCUS       DAOMSU010000104         6757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_913_length_6757_cov_2.880334, whole genome shotgun sequence.
ACCESSION   DAOMSU010000104 DAOMSU010000000
VERSION     DAOMSU010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_913_length_6757_cov_2.880334"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PK077_05735"
     CDS             complement(<1..417)
                     /locus_tag="PK077_05735"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRN23837.1"
                     /translation="MTRTATPPGKPSFNKEARGRVTPSMDLTRLTGSISGPAVFLCLL
                     SLFFAGCKKTEQVPHETPTIIYEHPVTMDIPVTGSWVGHLDGVDNASIVPQITGYLQT
                     QNYANGAIVKKGEVLFRIDDSTFRDQVSKAKATLAQA"
     gene            complement(447..3203)
                     /locus_tag="PK077_05740"
     CDS             complement(447..3203)
                     /locus_tag="PK077_05740"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HRN23838.1"
                     /translation="MKNAAHHQGLSEQQVQENRTRYGVNILTPPEKEPLWKQFLEKFS
                     DPIIKILLVALLLSVGVACYQFLTGAESASVFLEPAGILAAILLATCVGFAFEVSANR
                     KFEILNQVNDDTMVQVIRGGNICELPRRDVVVGDIVILNTGEEVPADGVLLEAVSLQV
                     NESTLTGEPLIGKTTDEEEFKKDATYPSNHVLKGTTVADGHGVMEVTSVGDRTEYGKV
                     YEGSQIDNKVQTPLNRQLSRLGDLITWASYAIAAFIVIGRLTLYFSHLPGPVEWLSAG
                     TYILNTVMIAVTVIVVTVPEGLPMSVTLSLALSMKSMLANNNLVRKMHACETMGAATV
                     ICTDKTGTLTRNQMNVYKADFYGLGDAAPAENELGNLIREGIAVNSTAFLDYADPEHI
                     RALGNPTEGALLLWLHGLGVNYLDLRENARVLEQLTFSTERKYMATVAESPLLGKKVL
                     YVKGAPEIVLGLCSTVLTPEGQVPAADMRPAIEEQLLAYQNQAMRTLGFAYRVLEDDA
                     PVFEDGRIIRKDLVYLGIAAISDPVRDDVPQAVKDCMDAGIRIKIVTGDTPGTAREIG
                     RQIGLWTEEDTPDRLMTGVEFEQTPDAELLDRVMDLKIMCRARPMDKERLVKLLQKKD
                     QVVAVTGDGTNDAPALNAAQVGLSMGDGTTVAKEASAITILDNSFMSISRAVMWGRSL
                     YRNIQRFIVFQMTINVAACLIVLIGAFLGTESPLTVTQMLWVNLIMDTFAALALASLP
                     PDERVMQDPPRKTTDHIITRPMGRNILGVGILFVAFLFGLLQYFKHADITSLTQFSLS
                     GYFRSYLDFSTPEHELTGYELSLFFTIFVLLQFWNMFNAKAFNTHQSAFARMGASIGG
                     FGLVALLILAGQYLIVTFGGKMFNVVPLSWTDWGLIFAGTSLVLWFGELARAFTPDKS
                     GARS"
     gene            3370..4023
                     /locus_tag="PK077_05745"
     CDS             3370..4023
                     /locus_tag="PK077_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23839.1"
                     /translation="MWNDLSHGFSVLADALAFLSRSWGFWVCAGSVAGLAAVYAWWRK
                     RLALWAFVLAFLSLLAVVGLACRQAVCQKTFLWIHEPDLQALSGKYEVESSPRERRFF
                     RPPELKRFFQVSSSTLLLNEDGTCEISSFPRPEAWGRWISRPEEREDLSVLEEDLVSS
                     CRGTWSLVKKGGYYGVSIAYSPGRSLMLYLGGEGAECLVMPVNPADPLEAVTFEKTF"
     gene            complement(4134..6671)
                     /locus_tag="PK077_05750"
     CDS             complement(4134..6671)
                     /locus_tag="PK077_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23840.1"
                     /translation="MKFFKTTIAMTTLLGCLPTSPAEVAFPGPAPGTASAEQSASGYT
                     LSNNILTAKFILRNGSLSFGGMTSKLGPVAKAGDELFIINLQDGRSLKSSELKAGNVK
                     FVSLQPNPKAFRLADRFPGKAVSATFQALNGTFRISWRAVLRDHSHYLRQELRLASTK
                     PVQMKNIVAMQYDLVEGKAGAPSVSGNARGALIVNDLAFTALETPMGINTVGGAGNME
                     GGETAIWSADKWSTASWTGNFNVPQELKKQYGEQVSSAEGPVSIDGKGACTVTFQYKG
                     GDKGNNKLNLAGVQLLSSKGAVLDSDFHNGSTGDSNAGNTYTVQVPSRGNYILRYWAQ
                     TKSEPIASKGEITFSLPVRTLAEKQEPQADESRLAQGLWSRKTTLQPGEIWDVSSVLG
                     IFAPGQKRRSFLAYMERERVMPYRPFIHYNSWYELNINRNNDSDPAKRMTEEQCLSVL
                     KDWQEQFYQKRGISIDAFVWDDGWDEFNSLWDFHKMFPQGFKRIDAAAGRQKAGIGTW
                     LGPVGGYGASKGKRLAYWNVKHPDNKIGNFQLSNKEYFDAFVGRCSQMVKDYNMKYFK
                     FDGISTHFHAKGPGNEEDAEGIIRVLNALRKKKGDLYINCTVGTWASPFWFRYADSVW
                     RQENDFGTIGAGDNRDKWITYRDRLVHEVFVQGSPLMPINSMMTHGLMVTKFGPPACM
                     PRDPENVKKELRCATACGTSLQELYVDRDLMNANGGVLWDELAKGIKWIRRNADVLDD
                     VHWVGGNPWNKETNEGAVYGWAAWNKNKATLALRNSSDQEKSLTGTLRSILDIPANVK
                     GSITFKDSYDDQRTLDGFSGSSVDIDKEISFTLKPFEVLVYEGGKVK"
BASE COUNT         1259 a         1846 c         2097 g         1555 t
ORIGIN      
        1 cgcctgggcc agggtggctt tggccttgga tacctggtcc ctgaacgtgg aatcgtcaat
       61 gcggaagaga acttcccctt tcttcacaat agcgccattg gcgtaattct gcgtttgaag
      121 gtagcccgta atctggggga cgatggaggc gttgtccacg ccgtccaggt ggcccaccca
      181 gctgcccgtc accggaatat ccatggtgac gggatgttcg taaataattg tgggcgtttc
      241 atgcggcacc tgttccgtct ttttacaccc ggcaaaaaag agggaaagca ggcagaggaa
      301 aacggcgggg ccggagatag agcccgtcag gcgtgtcaag tccatgctgg gagtgacacg
      361 cccgcgggct tctttgttga aagaaggttt tccgggcggc gtcgctgtcc gcgtcatgcg
      421 cggggggagg gattctcccg gacggttcag gagcgggcgc cggatttatc cggagtgaat
      481 gcccgcgcca gttccccgaa ccacaagaca agggaggtgc ctgcgaagat gaggccccag
      541 tccgtccagc tcaggggcac cacgttgaac atctttccgc cgaaggtgac gatgaggtac
      601 tgcccggcca ggataagcag ggctaccagg ccgaagccgc caatgctggc gcccatgcgt
      661 gcaaaggcgc tctggtgcgt gttgaatgcc ttggcgttga acatgttcca gaattggagc
      721 aggacgaaaa tggtgaaaaa cagggacagt tcataaccag tcagttcatg ttccggggtg
      781 gagaagtcca ggtagctgcg gaagtagccg ctgaggctga actgcgtgag actggtaatg
      841 tccgcatgct tgaaatattg cagcaggccg aacaggaaag cgacgaacag gatgcccacg
      901 cccaggatgt tccggcccat ggggcgggtg atgatgtggt ccgtggtctt gcgcggcggg
      961 tcctgcatca cgcgttcgtc cggcggcagg gaggccagcg ccagcgcggc gaaggtgtcc
     1021 atgatgaggt tcacccagag catctgcgtg acggtgaggg gggattccgt gcccaggaat
     1081 gcgccgatga gcacaatgag gcaggccgcc acgttaatgg tcatctggaa gacgatgaag
     1141 cgctggatat tcctgtacag ggaacgcccc cacatgacgg cgcgggaaat gctcatgaag
     1201 gaattgtcca ggatggtgat ggcgctggct tccttggcta cggtggtgcc gtctcccatg
     1261 gaaagcccca cctgcgcggc gttcagggcg ggggcgtcgt tggtgccgtc cccggtgacg
     1321 gccactacct ggtccttctt ctggagcagt ttgaccagcc gttccttatc catgggcctg
     1381 gcgcggcaca tgattttcag gtccatcacg cggtccagca gttccgcgtc cggagtctgc
     1441 tcaaactcca cacccgtcat cagacggtcc ggcgtgtcct cctccgtcca cagaccgatc
     1501 tggcgcccta tttcccgtgc ggtgccgggg gtatcccctg tgacgatctt gatgcggatg
     1561 cccgcgtcca tgcagtcctt cacggcttgg ggcacatcgt cccggacggg gtcggaaatg
     1621 gcggcgatgc ccagataaac cagatccttg cggatgatgc ggccgtcctc gaataccggg
     1681 gcgtcatcct ccagaacgcg gtacgcgaag cccagcgtgc gcatggcctg gttctggtag
     1741 gccaggagct gttcctcaat ggcggggcgc atgtctgcgg cgggaacctg gccttccgga
     1801 gtcaggacag tggagcaaag gcccagcacg atttccggcg cgcctttcac gtaaaggact
     1861 tttttgccca gcagggggga ttccgccacg gtggccatgt acttgcgttc cgtggagaaa
     1921 gtaagctgtt ccagcacgcg ggcgttttcc cgcaggtcca ggtaattcac gcccagcccg
     1981 tgcagccaca ggaggagcgc tccttccgtg gggttgccca gggccctaat atgttccggg
     2041 tccgcgtaat ccagaaaggc ggtggagttg acggcgattc cctcccggat caggttcccc
     2101 agctcgtttt ccgccggggc ggcgtccccc aacccgtaaa aatccgcctt gtacacgttc
     2161 atctggttgc gcgtcagggt gcccgtcttg tctgtgcaga tgacggtggc ggcccccatg
     2221 gtttcgcagg cgtgcatctt gcgcaccagg ttgttgttgg cgagcatgct cttcatgctc
     2281 agcgccaggc tcagggtgac gctcatgggc agcccctccg gcaccgtgac gacgatgacg
     2341 gttacggcga tcatgacggt attcagaatg taggttcccg cgctcagcca ttccacgggg
     2401 cccggcagat gggaaaaata cagggtaagc cgcccgatga cgatgaaggc ggcgatggcg
     2461 tagctggccc aggtgatgag gtcgcccagc ctggaaagct ggcggttgag gggcgtctgc
     2521 accttgttgt ctatctggga accttcataa accttgccgt attccgtcct gtcccccacg
     2581 gaagtcactt ccatgacgcc gtggccgtcc gccacggtgg ttcctttcag aacgtggttg
     2641 gaggggtagg tggcatcctt cttgaattcc tcttcatccg tggttttgcc gatgaggggt
     2701 tcccccgtca gcgtggattc gttgacctgg agggaaacgg cttccagcag gacgccgtcc
     2761 gccggtacct cctcccctgt gttcaggatg acgatatcgc ccaccaccac gtcccggcgg
     2821 ggcaattcgc aaatattgcc gccgcggatg acctggacca tggtgtcgtc attgacctgg
     2881 ttcaggattt caaacttcct gttggcgctt acctcgaagg cgaagcccac gcaggtggcc
     2941 agcaggatgg ccgccaggat gcccgcgggc tccagaaaga cgctggccga ttccgctccg
     3001 gtaaggaact gatagcaggc cacgcctacg gacaggagca gggccaccag cagaattttg
     3061 atgatggggt ctgaaaattt ctccagaaat tgcttccaca ggggttcctt ttccggggga
     3121 gtcaggatat tgaccccgta tctggtccgg ttttcctgaa cctgctgttc cgaaagcccc
     3181 tgatgatgtg ctgcattttt catggttcag gtgcttctta cctgattgga gcctgtgaga
     3241 aaagcgcgga ggaatatttt caggagtgat gacgggagtt ttttagcggg acgcagcctg
     3301 cggcccgtgc cttgaaaggc ggcattgttg tcttgacttt gactatgttc cggagattct
     3361 ggagcaccta tgtggaatga tctttctcat ggattttccg tgctggcgga tgccctggct
     3421 ttcctttccc gttcctgggg gttctgggtt tgcgcgggca gtgtggcggg gctggccgcc
     3481 gtttatgcgt ggtggagaaa gcggctggcc ctgtgggcgt ttgtcctggc gtttctttcc
     3541 ctgctggccg tggtggggct ggcgtgccgc caggcggtgt gtcaaaaaac tttcctgtgg
     3601 attcatgagc cggacctgca ggccctttcc ggaaagtatg aggtggaatc ctcccccagg
     3661 gagaggcgtt ttttccgccc tccggagttg aagcgttttt ttcaggtcag ttcttccacg
     3721 ctcctcctga atgaagacgg gacgtgcgag atttcttcct ttccccgtcc ggaggcatgg
     3781 ggcaggtgga tttcccgtcc ggaagagagg gaagaccttt ccgtgctgga ggaagatctc
     3841 gtttcttcct gccgcggaac ctggtccctg gtgaaaaaag gaggatatta cggtgtctcc
     3901 atcgcctatt cccccggcag gagcctgatg ttgtacctgg gcggggaagg tgcggaatgc
     3961 ctggtgatgc cggtcaaccc tgccgatccg ctggaagcgg tgacgtttga aaaaacattc
     4021 tgacccggat tctgtccgca cgggaacaag gaagggaggc gggaatcccg cctcccccaa
     4081 tatgtttttt ccaacgccgt tccggctttg accgggcgtg tgggccggct gatttatttg
     4141 acctttcccc cttcgtacac cagcacctca aagggcttca acgtgaagga gatttccttg
     4201 tcaatgtcta cggaactgcc ggagaagccg tccagcgtcc gctggtcgtc ataggaatcc
     4261 ttgaaggtga tggagccctt gacgttcgca gggatatcca gaatgctgcg cagggttccg
     4321 gtcaggctct tttcctggtc ggaggaattg cggagagcca gggtggcctt gtttttattc
     4381 catgcggccc agccgtacac ggctccttcg ttagtttcct tgttccacgg attgccgccc
     4441 acccagtgca cgtcgtccaa aacgtccgca ttgcggcgta tccacttgat ccccttggcc
     4501 agttcgtccc acaggactcc tccgttggcg ttcatcaggt cccgatccac gtatagctcc
     4561 tggagggaag tgccgcaagc cgtggcgcaa cggagttctt tcttgacgtt ttccggatcg
     4621 cggggcatgc aggccggagg gccgaattta gtgaccatca ggccgtgagt catcatggaa
     4681 ttgatgggca ttagcgggga gccctgcaca aaaacttcat gcaccaggcg gtcgcggtag
     4741 gtgatccatt tgtcgcggtt gtcgcccgct ccgatggtgc cgaagtcatt ctcctgcctc
     4801 catacggaat ccgcataacg gaaccagaag ggggatgccc aggttcctac agtgcagttg
     4861 atgtaaagat cccccttttt cttgcgcagg gcgttcagca cgcggatgat gccttccgca
     4921 tcctcttcgt tgccgggccc cttggcatgg aagtgggtgc tgatgccgtc gaatttgaag
     4981 tacttcatat tgtagtcctt caccatctgg gagcagcggc ccacaaaggc gtcaaaatat
     5041 tccttgttgg aaagctggaa attgccgatc ttgttgtccg gatgcttcac gttccagtaa
     5101 gcgagacgct tgcccttgga ggctccgtag ccccccaccg gacccagcca cgtgccgatg
     5161 ccggcctttt gcctgccggc ggcggcgtca atgcgcttga acccctgcgg gaacattttg
     5221 tggaaatccc acaggctgtt gaattcatcc cagccgtcgt cccagacgaa ggcgtcaatg
     5281 gatatgccgc gtttctggta aaactgttcc tgccagtctt tcaggacgga caggcattgt
     5341 tcttccgtca tgcgcttggc ggggtccgaa tcattgttgc ggttgatgtt cagttcatac
     5401 caggaattat aatggatgaa ggggcggtag ggcatcacgc gttcccgttc catataggcc
     5461 aggaaggagc ggcgtttctg gccgggggca aaaattccga gaacggagga tacgtcccat
     5521 atttccccgg gctgcaaggt ggttttacgg ctccagagtc cctgggccag ccgggattcg
     5581 tccgcctggg gttcctgctt ttccgccagc gtgcgcacgg gcagggaaaa ggtgatttcc
     5641 cccttgctgg caatcggttc gctcttggtc tgggcccagt agcgcagaat gtaatttccc
     5701 ctggagggaa cctgtaccgt gtaagtgttg ccggcattgg aatctcctgt gctgccgttg
     5761 tggaaatcgg agtcaagaac ggcccccttg gaagagagga gctgcacgcc ggcaaggttc
     5821 agcttattgt tgcccttgtc ccctccctta tactggaaag tgacggtgca tgctcctttg
     5881 ccgtcaatgg aaaccgggcc ttccgcactg gaaacctgtt ctccgtactg ctttttcagt
     5941 tcctggggaa cattgaaatt tccggtccat gaggctgtgg accacttgtc tgcgctccag
     6001 atggcggttt ctcctccttc catatttccg gcgcctccca cggtattgat gcccatggga
     6061 gtttccaggg ctgtgaacgc caggtcattg acgataagtg cgcctctggc gtttccggaa
     6121 acggagggag ccccggcttt cccttctacc aggtcatatt gcatcgctac gatgtttttc
     6181 atctggacgg gcttcgtact tgccaggcgg agttcctgcc gcaggtagtg ggaatgatcc
     6241 ctgagcacgg cgcgccagga aatgcgaaag gttccgttca gagcctggaa tgttgcgctg
     6301 acggcttttc ccgggaagcg gtcagctagc ctgaatgcct tggggttggg ctgaagggag
     6361 acgaatttga cgtttcccgc tttcagctcg gaggatttaa ggctccgtcc gtcctgaaga
     6421 ttgatgatga ataattcatc ccctgccttg gcgacggggc ccagtttgga agtcattcct
     6481 ccgaaggaaa gacttccgtt ccgaaggata aatttggccg tcaggatgtt attggaaaga
     6541 gtatagccgg aagcggattg ttccgcgctg gctgttccgg gtgcaggccc cggaaaggcg
     6601 acttctgcgg gggatgtggg caggcagccc agcagggttg tcatggcaat ggttgtcttg
     6661 aaaaatttca tggtgaaata atattacagc tagcgggagg ataatttggg gaaggatctt
     6721 tttcaagaaa aaggtcctgg aaatggagtg aaagaaa
//
LOCUS       DAOMSU010000105         6743 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_915_length_6743_cov_2.470096, whole genome shotgun sequence.
ACCESSION   DAOMSU010000105 DAOMSU010000000
VERSION     DAOMSU010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_915_length_6743_cov_2.470096"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..1350
                     /locus_tag="PK077_05755"
     CDS             208..1350
                     /locus_tag="PK077_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HRN23841.1"
                     /translation="MSNDTFSERLAYPKGLKGIIANESALSDVRGEEGRLLYLGYDID
                     DLVEMCCFEEVVYLLLNKRLPNREELEGIKKRLRSDRDLPQPILDFFKTATKSARPMS
                     ALRTAVSMLGMYDDRTKDAGQLKEIGLSLIAKVPVMVAYYYRLCQGLELPPVREDLSE
                     AEHFLWLLKGEEPDQYEAHILEVAYILHADHGMNASTFAARVCIATLSDMYSAITAAI
                     GTLKGPLHGGANEGVIEMLKEIGTEDRVDSYIEDKLARKEKIMGMGHRVYRVLDPRAP
                     HLRRMAIRLSSRIGEPKWIRMSERIAKLVRERKGLNANVDFYSATVYYSIGIPTRLFT
                     AIFSIARCSGWVAQVLEQMEDNTLYRPLTLYTGPKDRIPVPTIDMR"
     gene            1402..1608
                     /locus_tag="PK077_05760"
     CDS             1402..1608
                     /locus_tag="PK077_05760"
                     /inference="COORDINATES: protein motif:HMM:NF021511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HRN23842.1"
                     /translation="MCLPLFFFVKISFLSAGGIQPYCSLSWAFSIMAVAAAYEIIEWQ
                     YAVIVGGDEADDFFSSLGGYTGFP"
     gene            complement(1657..1812)
                     /locus_tag="PK077_05765"
     CDS             complement(1657..1812)
                     /locus_tag="PK077_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23843.1"
                     /translation="MKDCARPAFDESPSLPLHIGMDVLTAGSTTKKYRAMKRRFLRLS
                     SVRGISR"
     gene            complement(1784..2248)
                     /locus_tag="PK077_05770"
     CDS             complement(1784..2248)
                     /locus_tag="PK077_05770"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HRN23844.1"
                     /translation="MQPRFHIVMFHPEIPHNTAAAGRLALATGSRLHLIKPLGFSLDE
                     KHVRRTGLDYWAKVDLHVWDSLEELKQAAAPGAQFWYLSTKAPRSHWDAVFHAGDYLV
                     FGPESRGLPESMLKEHADTALTIPMPGEGARSLNLSTAIAVVLYEGLRQTGI"
     gene            2302..2892
                     /locus_tag="PK077_05775"
     CDS             2302..2892
                     /locus_tag="PK077_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRN23845.1"
                     /translation="MANNVRFHPMRDIVYFDLETRHSAAEVGGWHNTAEMRLSVGVTY
                     STASGKYTIYSEEMVDDLIRQLSQADLVVGYNHEHFDYGVLQRYTMWNMIDITNNLDL
                     CKDIEQRGGVRVKLDSVAAASIGSSKTAVGTQALKWWAEYVQTGNTELLMDIARYCCF
                     DVKVTRDVHWYGAEHGFIRYDDKKGGQVELPVDWKL"
     gene            complement(3069..3527)
                     /gene="smpB"
                     /locus_tag="PK077_05780"
     CDS             complement(3069..3527)
                     /gene="smpB"
                     /locus_tag="PK077_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRN23846.1"
                     /translation="MSSDISTNKKARRDYEILDTYECGMELKGTEVKSIRAGKVNIAD
                     SFARVENGQMLLYGCDIQPWETAGEFFQHQARRPRRLLLHKREIFKLEQQTSQKGCSL
                     VALKLYWKNGKVKLALGLGKGKTHRDQRYDLKARVEMREAQREVARINRR"
     gene            complement(3642..3863)
                     /locus_tag="PK077_05785"
     CDS             complement(3642..3863)
                     /locus_tag="PK077_05785"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HRN23847.1"
                     /translation="MKAELVEQASKIIPEPQMLINVVSRRVAQLNNGRAPLVPTTPHM
                     GNANIALTEIIEGKLVYHTESGSLQEDNQ"
     gene            complement(3938..4546)
                     /locus_tag="PK077_05790"
     CDS             complement(3938..4546)
                     /locus_tag="PK077_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRN23848.1"
                     /translation="MPKPAVHATLAVHGMILAFNKPYGILSQFTKEAPHHRTLAEFGF
                     PPGVYPVGRLDADSEGLLLLSDEKGLADRLLNPSGRHPRTYWSQVEGTPSETDLRPLE
                     LGGLRIRGHCTLPCRARLMRREPDVPPRIPPVRYRASIPTSWLELTLVEGKNRQVRRM
                     TAAIGFPTLRLLRARIGNFSLAGLKPGAWKELDSRERRQLMP"
     gene            4628..5386
                     /locus_tag="PK077_05795"
     CDS             4628..5386
                     /locus_tag="PK077_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23849.1"
                     /translation="MLACCVPVWVQAAPDTGEVKAKIARKIWQNECAGTIRGLVSWNR
                     GEAFPSLGIGHFIWFPAGVTERFEESFPAFIQFCRRKGIRVPEWFSGVAPWRTRKEFE
                     AADVRGGLPERMRRWLSSPAALQMQADFIIARSVAALERIKGQSRRPEEMAARYYAVA
                     SVPNGMYALIDYVNFKGEGTNPAEQYRGIGWGLRQVLEEMRPVSPGQPAAVEFAEAAK
                     RVLQRRVDNSPPGRGEARWLAGWRNRCDSYKRNL"
     gene            5490..5945
                     /locus_tag="PK077_05800"
     CDS             5490..5945
                     /locus_tag="PK077_05800"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRN23850.1"
                     /translation="MPNTQEETTLILLKPDCVRKNLSGTILKRFLSEGFRLRGIKMIQ
                     LDEPVLREHYAHILDLVINGEPLFPKLLDFMTSSPVIAIALKGPGVIVRVRELLGPTN
                     SQKAEKGTIRGDFGTDSMMNICHASDSRESAAVELARFFREEELFDGLN"
     gene            complement(6092..>6743)
                     /locus_tag="PK077_05805"
     CDS             complement(6092..>6743)
                     /locus_tag="PK077_05805"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN23851.1"
                     /translation="LKLHVEKDSGMSEGINRGFRKARGKWVMWLNTDDRLLPGALAAV
                     KAFADSRPDAGIVYGAWNFIGPDGEIQRAMKALPYSLNMHIWYGTYLASTALFLRRST
                     TIEEGFLLDERFHYDMDGEYYARLGRAGKKFVHYNRLLADFRWHGDNLSAPNIERRDM
                     DAELKRQKQHGEDAAIKRIYGHSFSKHSCNNIMDGFMREAYRMKKAFLYLTTPWEK"
BASE COUNT         1409 a         1745 c         2027 g         1562 t
ORIGIN      
        1 gcgctcgggg ccgcgtggct gtccctggcg gaggcccgca gagaagaact tctggaaagt
       61 ccggcggaac cgctgtatgt gagggctccg cacatcaccc gcgccaaacg tcccgcatgg
      121 gccgtgaagg cgtaattaag gtttgacttt ttggaagatt gttagacttt taaccggtaa
      181 tattcaccac cagtagcatt ttttaacatg agcaatgaca ctttttccga gcgcctcgca
      241 tatcccaaag gtttaaaagg aatcatcgcc aatgaatccg ctttgagtga cgtgcgcggc
      301 gaggaagggc gtctgttgta tctgggctac gatatcgatg atctggtgga gatgtgctgt
      361 tttgaagaag tggtttatct gcttctcaac aagcgtcttc ccaaccggga ggaattggag
      421 ggcatcaaga agcgcctccg ttccgaccgc gacctgcccc agcccattct ggatttcttt
      481 aaaacggcca ccaagtccgc ccggcccatg tccgccctcc ggacggctgt ttccatgctc
      541 ggcatgtatg acgaccggac gaaggatgcc gggcagttga aggaaatagg cctcagcctc
      601 atagccaagg ttcccgtgat ggtggcgtat tactaccgtt tgtgccaggg tctggagctt
      661 ccccccgttc gggaggacct gagcgaggcg gaacatttcc tgtggttgct gaagggtgag
      721 gaaccggatc agtatgaagc ccatattctg gaggtagcct acattttgca cgcagaccac
      781 ggcatgaatg cctccacctt tgcggcgcgt gtctgcatcg ccacgctttc ggacatgtat
      841 tccgccatca cagcggccat cggcacgctg aaagggccgc ttcatggggg cgccaatgag
      901 ggcgtgattg aaatgctcaa ggaaataggc acagaggacc gggtggactc ctacattgag
      961 gacaagctgg cgcggaagga aaaaatcatg ggtatgggcc accgcgtgta ccgcgtgttg
     1021 gatccccgcg ccccgcattt gcgacgcatg gccatccgcc tttcctcccg aatcggggaa
     1081 cccaagtgga tccgcatgtc ggaacgcatc gcgaaacttg tccgggagcg caagggcttg
     1141 aacgccaatg tggattttta ttccgccacg gtttattaca gcatcgggat ccccaccagg
     1201 ctgtttaccg ccattttctc cattgcccgc tgtagcggct gggtggccca ggttctggaa
     1261 caaatggagg ataacacgtt gtacaggccg ctgactcttt acacaggacc caaggaccgc
     1321 attccggttc ctacgatcga catgcggtga caggttttaa tcaggaaagg cgccggatat
     1381 tacttccggc gcctttcctg catctgcctt ccgcttttct ttttcgtgaa aatatccttt
     1441 ctatccgccg gaggtattca accgtactgt tcattgtcct gggctttttc cattatggcc
     1501 gtagcggcag cttatgaaat tattgaatgg caatatgccg ttatcgtggg tggggatgaa
     1561 gcagatgatt ttttcagttc cctgggaggc tatacgggat tcccataagg gtatgttctg
     1621 ggatatctgc tgagccgtct ctttcttgat tctcttttat ctggatatgc ccctgacgga
     1681 ggaaagacgc agaaaccgcc tcttcatggc cctgtatttt ttggtggtgg agcccgctgt
     1741 aagaacatcc atgccgatgt ggagaggcag ggagggggac tcgtcaaatg ccggtctggc
     1801 gcagtccttc ataaaggacg acagctatgg ctgtggatag attcaggctg cgggcgcctt
     1861 ctccgggcat cggaatagtc agggcggtat ctgcgtgttc tttcagcatg ctttccggca
     1921 atccacggga ttccggtcca aaaaccagat agtcccctgc gtgaaaaacc gcatcccaat
     1981 gggaacgtgg cgccttggtg ctcaggtacc agaattgcgc tccgggggct gccgcctgct
     2041 tcaattcctc caggctgtcc cagacatgca gatccacttt ggcccaatag tccagccccg
     2101 tacggcgcac atgcttttca tccaggctga accccaaagg tttgatcaga tggagcctgg
     2161 aacccgttgc caaagccaac cgtccggcag ctgccgtatt atgcggaatt tccggatgaa
     2221 acatgacgat gtggaaacgg ggctgcatgt gcttattctc atcacaaaca tgcctcccgt
     2281 tcaagtggga cttgtccaaa agtggcaaat aatgtacgtt ttcacccgat gagagacatc
     2341 gtgtattttg atttggaaac ccgtcattcc gcggcggagg tgggcggctg gcacaatacg
     2401 gccgagatgc gcctttccgt gggcgtgact tattccacgg cttccggcaa atatacgatt
     2461 tattccgagg aaatggtgga tgatctcatc cggcagcttt cccaggccga cctggtggtg
     2521 ggttacaacc acgagcattt tgactatggc gtgctccagc gttatacgat gtggaacatg
     2581 attgatatca cgaacaacct tgatttgtgc aaggacatcg aacagcgcgg gggcgtgcgc
     2641 gtcaagctgg attccgtggc ggctgcctcc atcggttctt caaaaacggc cgtgggcacc
     2701 caggctctca aatggtgggc ggaatacgtg cagacgggca atacggagct tttaatggat
     2761 atagcccgtt attgctgttt tgacgtgaag gtgacgcggg atgtgcactg gtacggagcg
     2821 gaacacggtt tcatccgtta tgatgataaa aagggcggcc aggtggaatt gcccgtagat
     2881 tggaaattgt aactttttct gcgggcatgc ctggccggtt cttttcccgg gcgctggagt
     2941 gaggctggtt tccctccttt ggcggcgttg ccgtttatcc cgggcagata ccggttatca
     3001 gggaatggct gggacagaga aaggcttccg ggaagaagcg gacggcaact gcgcggttgc
     3061 cgcctgactt accggcggtt gatgcgggcc acttcccgct gggcttcgcg catttccacg
     3121 cgggctttca aatcgtagcg ctggtctcgg tgggttttgc ccttgcccag gcctaaagcc
     3181 agtttcacct tgccgttttt ccaatacagc ttgagagcta ccagggagca gcctttctgg
     3241 gaagtctgct gttccagttt gaaaatttcc cgtttatgga gaagaaggcg gcggggccgg
     3301 cgcgcctggt gctggaagaa ttctcccgct gtctcccagg gctggatgtc gcagccgtac
     3361 aggagcatct ggccgttttc caccctggcg aaagaatcgg caatattcac cttgccggcg
     3421 cggatggatt tgacttccgt tcccttgagt tccatgccgc attcgtaggt gtccagaatt
     3481 tcgtaatccc ggcgggcttt tttgttggtg gaaatgtcgg agctcatggc acgaagggga
     3541 gaaggatgaa aaggccccgg gctgggagac cggggcctgg ctggatagag aggattccgt
     3601 acggaattcc ggtccggaac ggcaataaag ggaatgtgtt gttactggtt gtcttcctgg
     3661 aggctgccgg attccgtatg atagacgagc ttgccttcaa tgatttccgt cagggcaatg
     3721 ttcgcgttgc ccatatgggg tgtggtaggc accagcgggg cgcggccatt attaagctgg
     3781 gctacgcggc gggaaaccac gttgatcagc atttggggct cgggaatgat tttggaggct
     3841 tgttcgacga gttcggcttt catgtaaaaa ggtctaggta gatatcctga tgcggagaga
     3901 gatataaaaa cggttcctgt ccggaatcaa gcttatatta cggcatgagc tgccttcgct
     3961 cgcgggaatc cagttctttc catgcgccgg gtttcagtcc cgccagggag aaatttccga
     4021 ttctggcgcg aagaagccgg agtgtgggaa aaccgatggc ggcggtcatg cgccgcacct
     4081 ggcggttttt cccttccacc agcgtcaatt ccagccatga ggtgggaatg gaggcgcggt
     4141 agcggacggg agggatgcgg ggcggcacgt caggctccct gcgcatgagg cgtgcccggc
     4201 aaggcagcgt gcagtgcccg cggatgcgga ggcccccgag ttccagaggg cgaaggtccg
     4261 tttcggaagg agtgccttcc acctggctcc aataagttct gggatgcctg ccggaggggt
     4321 tcaggagacg gtccgccagg cccttctcat cggataaaag aagcagccct tccgaatccg
     4381 catccagcct tcctacagga tacacgccgg gaggaaaccc gaattcagcc agtgtccggt
     4441 ggtggggagc ctccttcgta aattgggaga gaataccgta aggtttattg aacgccagaa
     4501 tcatgccgtg cacggcaagg gtagcatgaa ccgctggctt tggcacggga aattgaagcg
     4561 gcttgacttg tctgtgttgg ggaaggaaag tatctgcatg catggaaaaa cggcaggttg
     4621 tgtcctgatg cttgcgtgtt gcgtgcccgt ctgggttcag gcggcgccgg atacggggga
     4681 agtgaaggca aaaattgcgc ggaaaatctg gcaaaacgaa tgtgccggga caataagggg
     4741 gctggtttcc tggaaccggg gagaggcgtt tccatccctg ggaatcggac atttcatctg
     4801 gtttcctgcc ggagtcacgg aaaggtttga ggaaagtttc cctgctttca ttcagttctg
     4861 ccgcaggaag ggaattcggg ttccggaatg gttttccggt gtggctccgt ggcggaccag
     4921 aaaggaattt gaagcggcgg acgttcgcgg cggtcttcct gaacggatgc gccggtggtt
     4981 gtccagcccg gcggccctgc aaatgcaggc ggatttcatt attgccaggt ctgtggccgc
     5041 tctggagcgt atcaagggcc agtcccgccg tccggaagag atggcggccc gctattacgc
     5101 cgtggcgtcg gttcccaacg gcatgtatgc cctgattgac tacgtgaatt tcaagggaga
     5161 aggaaccaat ccggcggaac agtaccgggg gattggctgg gggctcaggc aggtgctgga
     5221 ggaaatgcgc cccgtttctc ccggacagcc tgcggcggtt gaatttgcgg aagcggccaa
     5281 aagggtgctt cagcgccgcg tggacaacag tccgccgggc cgcggagaag cgcgctggct
     5341 tgccggatgg cggaaccggt gcgattccta taagaggaat ttgtgacgaa gtcctttccc
     5401 aacagaaaag accagttgtg caattatcct tgacctgacg gggattaggg tgtaattccc
     5461 tcactccatc ctgaaacttc gctgatttta tgccaaacac tcaagaagaa acaacgctga
     5521 ttttgctgaa gcccgactgt gtgcggaaaa acctttccgg caccattttg aaacgttttt
     5581 tgtccgaagg gtttcgcctg cgcggcataa agatgatcca gcttgatgaa ccggtgctcc
     5641 gggaacatta cgcccatatt ctggacctgg tcatcaatgg ggaaccgctt ttcccgaagc
     5701 tgcttgattt catgaccagt tctccggtca ttgccattgc cctgaaaggg cccggcgtta
     5761 ttgtgcgcgt gcgcgaactg ctggggccca ccaattccca gaaggcagag aaaggcacaa
     5821 tccgcggtga tttcggaaca gacagcatga tgaacatctg ccatgcgtcc gacagccgtg
     5881 aatccgccgc cgtggagctg gcaagatttt tccgggaaga agaactgttt gacggcctta
     5941 attaagggaa ggccgccttt ttcccatcag gttaattgag ggccgcagct gtaataacgg
     6001 ctgtggccct tgccgttgcg aggctgtacc tcccatggga ggatgaaggg gggaggcagc
     6061 ggcttttccg ggagaagcag gaaagaacgg gttacttttc ccagggagtg gttaaataaa
     6121 ggaaggcctt tttcatacgg taggcctcac gcatgaagcc gtccatgata ttgttgcagc
     6181 tgtgtttgga aaaggaatgg ccgtaaatgc gcttgatggc ggcatcctct ccgtgctgct
     6241 tctggcgctt taactcagcg tccatgtccc ggcgttcgat attgggggcg ctcagattgt
     6301 cgccatgcca gcgaaaatcc gccagcagcc tgttgtaatg cacgaacttc ttccccgccc
     6361 ggcccaggcg cgcgtaatat tccccgtcca tgtcgtagtg gaaacgttcg tccagcagga
     6421 acccctcttc aatagtagtg ctccggcgca ggaacaatgc ggtggaagcc agataggtac
     6481 cgtaccagat gtgcatgttc agactgtagg gcagggcctt catggcgcgt tggatctctc
     6541 catccggtcc gatgaaattc catgctccat acacaatgcc cgcatctggg cgggaatccg
     6601 caaaggcctt gaccgccgcc agagcccccg gcaacagccg gtcgtccgta ttcagccaca
     6661 taacccattt cccgcgcgcc ttgcggaagc cgcggttaat gccttccgac atgccggaat
     6721 ccttttccac atgcagtttc agg
//
LOCUS       DAOMSU010000106         6738 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_916_length_6738_cov_2.601227, whole genome shotgun sequence.
ACCESSION   DAOMSU010000106 DAOMSU010000000
VERSION     DAOMSU010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6738
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_916_length_6738_cov_2.601227"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            46..564
                     /locus_tag="PK077_05810"
     CDS             46..564
                     /locus_tag="PK077_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HRN23852.1"
                     /translation="MQTGILRGMSVLAGLLWLASCSSSPKSRDYPGYMTRPYTIRGHR
                     YHPMNVEQALTYEQTGIASHYNECALWGLVSGKTAIGENVRPWHLHAAHPTLPLPCEV
                     LVQSLRTGKTVKVRVNDRGPFIKNRIIDLSEEAAERLDMKHHGLDKVRITVLSVGDGK
                     WKKEASPCATPA"
     gene            complement(569..>1148)
                     /locus_tag="PK077_05815"
     CDS             complement(569..>1148)
                     /locus_tag="PK077_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HRN23853.1"
                     /translation="AGVYVDGGIGAALRKLGVCDSKLVGSSSRIRCLAEGIRRVPGIR
                     FHLVSIGPERYNQLYPAFGNLNRFLAWGHATVIEGLAAKVPDCPMALSDQFANPFVLK
                     RALAAKKVSVRLEQRVRAESDVAVAAASILARERFVNWMDAAGEGAGMKLPLGASGQV
                     VKAARQLVALHGEEMLPKVAKMHFKTTRNVLK"
     assembly_gap    1149..1194
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1195..1471)
                     /locus_tag="PK077_05820"
     CDS             complement(<1195..1471)
                     /locus_tag="PK077_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HRN23854.1"
                     /translation="MLTDAQAGRLESILEEKGFERREVPYARFSFAGPSLLATVYEKK
                     NKLLLQGKGTDDFIEFTLEPQVTGVLSQPEEMEEGVDFVPHFGIDESG"
     gene            1660..1992
                     /locus_tag="PK077_05825"
     CDS             1660..1992
                     /locus_tag="PK077_05825"
                     /inference="COORDINATES: protein motif:HMM:NF037081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRN23855.1"
                     /translation="MHSYHSALHMAKTLTKRDLVNKISAETNFTQIEVFDIVQRAVDI
                     ISSTLAEGDRVVIRNFGTFQVKEVKPKVGRNPKNPDQDVPIPARSVVKFKVGKELKDQ
                     VAKLTASK"
     gene            2099..2731
                     /locus_tag="PK077_05830"
     CDS             2099..2731
                     /locus_tag="PK077_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23856.1"
                     /translation="MFRIQYLLLIAAAALCSCATERTVATNFQPRQRPDSEKTDAELM
                     DALEAKFAGGFGQQTDKEGNTRMVSQKRSSFEGLRYSGNTSQIEKKAFETASFEKKKF
                     DGASTRFETKKWDGIKSFTDDKLETPDFIARAKGVNTHSWQDASKQYATQQSDFHGRS
                     WKEADKRFDRTVNRDIEAKRQSFGQPTIMSARQAQAHTIQETREMMGRTD"
     gene            2811..3074
                     /locus_tag="PK077_05835"
     CDS             2811..3074
                     /locus_tag="PK077_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23857.1"
                     /translation="MSAKDVPPSITLPTSDYHTIVKMSNHAVVGVFRQHVFARRSTRR
                     YAPPIPEEHDSYCVKRTPERVMVQIFHDGNEVYRCIFVPPADY"
     gene            3088..3531
                     /locus_tag="PK077_05840"
     CDS             3088..3531
                     /locus_tag="PK077_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23858.1"
                     /translation="MKAPFFSPAFCLITSLITACSSPSGPNAWTPVTSGPPRAPIPQN
                     EVLVIDEYPMGEYTNVGHFNGPEGRIVHVSMEDKELIDYFTKEAAAMGGNTVVIREPR
                     IRYRSGEGKSSRVDVIYVREEMGTHGAEDLGMDSLPIDEESLRIY"
     gene            complement(3795..4559)
                     /locus_tag="PK077_05845"
     CDS             complement(3795..4559)
                     /locus_tag="PK077_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23859.1"
                     /translation="MKMNKKSRDKIFDLHLGLTFDWRETGLLRWRIFLWMLFFGVCAF
                     AAGLFVHVEVPEIRSRGDQPGAVVLLPADNPRLHQYVLRNTPLPVRGPVWADPVTAGP
                     TGEMGVTLPSPRSADTPLAPLPPVSVPTVADQVWRMWQHPDFHIRAMMNPSLEISGTC
                     IPYLSFCSPGLDGRIVTAGSLSGFSRSEDHTGLRSDFFVVVDVWGVPSQVLLLESSGS
                     NGADESAMRFARSLRWVPSREPRSGTMSIEWKEEEP"
     gene            complement(4579..5010)
                     /locus_tag="PK077_05850"
     CDS             complement(4579..5010)
                     /locus_tag="PK077_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23860.1"
                     /translation="MQIKTRSTLPAGAFFIQVVVLLDLVVLVCVLTIMTSRVGMAYGY
                     EVKAPSSHFLMNIVGDVEFISVTAGDQPAFYIGNRLIEGGLPGVSSELDRISREHGEN
                     GSRMGIALRLDDSVSRALEQQLVDMVLAKGMTCYIAAEPAD"
     gene            complement(5035..5469)
                     /locus_tag="PK077_05855"
     CDS             complement(5035..5469)
                     /locus_tag="PK077_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HRN23861.1"
                     /translation="MVSSVLAHAGLRREELRTVAEDSVQMEVFQIEKNIRGLLVVATV
                     SPLVGVLGTIQGLVGFYSQPGVLEGKAPSLAMSDAVYQALLSSALGLSIAIPAYLFYS
                     YLASRSRQIVHSLERAGTEAVCLVCDARQRREAGESMNGRGV"
     gene            complement(5737..6444)
                     /locus_tag="PK077_05860"
     CDS             complement(5737..6444)
                     /locus_tag="PK077_05860"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HRN23862.1"
                     /translation="MRGVLRSSRPANIPTLFTNAAAAWASVRGAELPPLALYGGVVLM
                     GLCFYLYGMWENDRVDARWDAARYPDRPVPCGAVSVSVLRLLSLAAGLSGLVLNMALG
                     GEFAVGALLLIVISLYNVFHKLWSGSIVLMGLCRGVWVLAAGLAFARSGGESVPPPAL
                     LWYAFGLFLFTCVISAVARREAGRPQVQRAVTVLLSGMCLFDAVWLLSFGSLLWLGSV
                     LLWAGTRLLQKLGCRAT"
BASE COUNT         1765 a         2105 c         1585 g         1237 t
ORIGIN      
        1 cctgcgtctt gcatttcatc atctccccat ccatataata aaatcatgca gacaggtatc
       61 ctcaggggaa tgtccgtgct ggccgggctg ctatggctgg catcctgctc gtcctctccc
      121 aaaagccggg attatccggg ctacatgacc cgcccctata ccatccgggg gcaccgctac
      181 catcccatga acgtggaaca ggcgctgacg tatgaacaaa cgggaattgc ctcccattac
      241 aatgaatgcg ccctttgggg gctcgtcagc ggaaaaacgg cgatcggaga aaacgtgcgc
      301 ccctggcacc tgcacgcagc gcatcccacc cttcccctcc cgtgcgaagt gctcgttcaa
      361 tcccttcgaa caggcaaaac cgtaaaagtc cgcgtaaacg accggggccc cttcatcaaa
      421 aacagaatca tagatcttag tgaagaagcc gcagaacggc tggacatgaa gcaccacgga
      481 ctggacaaag tcagaatcac cgtcctttcc gtaggagacg ggaaatggaa aaaagaagcg
      541 tccccttgcg ccactccggc ctgattccct acttcaacac attccgtgtg gtcttaaaat
      601 gcatcttggc caccttgggc agcatttctt ccccatgcag ggccaccaac tggcgcgcag
      661 ctttcaccac ttggccggaa gctcccagcg gcaacttcat gccggcgccc tctcccgccg
      721 catccatcca attcacaaaa cgttcgcgcg ccagaataga tgccgcagcc acagcaacat
      781 cgctctccgc ccggacacgc tgttccagcc ggacagacac ctttttggcg gcaagcgccc
      841 tcttcagaac aaacggattg gcgaactggt cgctcaatgc catgggacaa tccggaacct
      901 tggccgccag cccctcaatc accgtagcat gcccccaggc cagaaaacgg ttcaaattcc
      961 cgaacgccgg ataaagctgg ttataccgtt ccgggccaat gcttaccaga tgaaaccgga
     1021 tgcccggcac cctgcggatc ccctccgcca ggcagcggat tctggatgaa gaccctacca
     1081 gcttgctgtc acaaacaccc agcttgcgca atgcggcgcc gatgcccccg tccacataaa
     1141 caccagccnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttccgc
     1201 tttcgtctat cccgaagtga ggaacaaaat cgactccttc ttccatctct tccggctggg
     1261 acaaaactcc ggtcacctgc ggttccagcg taaactctat aaaatcatcc gttccctttc
     1321 cctgcagaag aagcttgttc ttcttctcgt aaaccgtcgc cagcagggaa ggcccggcaa
     1381 aggaaaaacg cgcatagggc acctctcttc tttcaaaccc cttttcctca agaatggact
     1441 caagccttcc ggcttgggcg tcagtcagca tggttgtaaa cgtggttcgc atatatcctt
     1501 cccggagccg gaacactccg gggcaagcaa tggaagcatc ccaaccattc cggtcaagca
     1561 aaattcctgc atcacgcagt tgagaacgta aaaatcaagc atgccacgga ttatgtgatt
     1621 ttttaggaga caaatcatgc acgctgaact ataaaactca tacattcata tcattctgca
     1681 ctccatatgg ctaaaacatt aacaaaacga gacctcgtta acaaaatcag cgccgaaacc
     1741 aacttcactc agattgaagt gttcgacatc gtacagcgtg ccgtggacat tatctccagc
     1801 acccttgctg aaggcgaccg tgtggtgatt cgcaatttcg gaaccttcca ggtgaaggaa
     1861 gtgaagccca aagtaggccg caaccccaaa aaccctgacc aggatgtgcc gattccggcc
     1921 cgctccgttg tcaaattcaa ggtcggcaaa gaattgaagg atcaggtcgc caagctgacc
     1981 gcctccaaat aatccccaca gggatcagac agattactcc gggcgcctcc atcaagcaat
     2041 ggaggcgcct ttttgtttgc aatttgcgcg gcattcggga atataaggga acgtgagcat
     2101 gttccggata cagtaccttc ttctgattgc cgcagcagca ctttgctcct gtgctacaga
     2161 acgcacggta gctacaaact ttcagccccg gcaacgccct gattcggaaa aaacggacgc
     2221 agagctgatg gatgccttgg aagcgaaatt cgccggcggc ttcggccagc agacggacaa
     2281 ggaaggaaac accaggatgg tgtcccaaaa acggagttcc tttgaaggac tccggtacag
     2341 cggcaacacg tctcaaatcg aaaaaaaggc ttttgaaacc gcatcctttg aaaaaaagaa
     2401 atttgacggc gcctctaccc ggtttgaaac gaagaaatgg gacggcatca aatccttcac
     2461 ggatgacaag ctggaaacgc cggacttcat cgcccgcgcc aagggagtaa atacccactc
     2521 ctggcaggat gcctccaaac aatacgccac gcagcaatcc gactttcatg gacgctcctg
     2581 gaaagaggcg gacaaacgct tcgaccgcac tgtcaaccgg gacattgagg ccaagagaca
     2641 aagctttgga cagccaacta tcatgagtgc gcggcaggcc caggcgcata ctattcagga
     2701 aacgcgggaa atgatgggac gcacggactg atgtgccgat gtttcggacg tgatcccatg
     2761 ccacatttca gattcgacat tgttttcagc cgccatctat aattctgccc gtgagtgcca
     2821 aagacgttcc tccttccatc accttgccta ccagcgacta ccataccatt gtcaaaatga
     2881 gcaatcatgc cgtcgtggga gtattccgcc agcatgtctt tgcgcgccgc agcacgcgcc
     2941 gttatgcgcc ccccatccct gaagaacacg actcctattg cgttaaaaga actccggagc
     3001 gtgtcatggt ccaaatcttt catgacggca acgaagttta ccgttgcatt tttgtgccgc
     3061 ctgccgatta ttgacggcag gacaaccatg aaagctcctt ttttcagtcc ggccttctgc
     3121 ctcattacaa gcctgattac agcctgctct tctccctccg gccccaatgc gtggacgccg
     3181 gtcacttccg gtcctccgcg tgcgcccatc ccccagaacg aagttctggt cattgacgaa
     3241 taccccatgg gggaatacac caacgtgggg cattttaacg ggccggaagg gcgcattgtc
     3301 catgtctcta tggaagacaa ggaactaatc gactacttca ccaaggaagc tgcggccatg
     3361 gggggcaata ccgtcgtcat cagggaaccg cgcatccgct accgttccgg cgaaggcaag
     3421 agcagccggg tggacgtcat ctatgttcgg gaagaaatgg gaacacacgg tgcggaagac
     3481 ctgggcatgg actcgctccc cattgatgaa gaatctctgc ggatttatta gagccgccgg
     3541 aaacgtccca ggtgccattt cctgcccgga gttttttcag ctctttttat tggccgcacc
     3601 gcaatgcggg cactccgcac gggaattccc cttctccata taaaaccgga atccgcatat
     3661 ccggcactgg aacttgcgcc tccgctccag gaaagatcgt atccatccca cagccaggca
     3721 aacagatacc acagcgcaaa aaccgccggc gcccagtaaa aacaggcgca tcaaaaaatc
     3781 ctggagggtc atattcacgg ctcttcctcc ttccattcga tggacatggt gccgctccgc
     3841 ggctcccggg acggaaccca gcgcaaagac cgggcgaacc tcattgcgga ctcatccgct
     3901 ccattacttc cggaagactc cagcaacaaa acttgagaag gaacgcccca tacatccacc
     3961 accacaaaaa aatcactcct cagccccgtg tgatcttcac ttctggaaaa accggacagg
     4021 gagccggctg taacaatccg tccatccagc cccggggaac aaaaagacaa atagggaatg
     4081 catgttccgg aaatctccaa ggaaggattc atcatggcgc gtatatgaaa atccggatgc
     4141 tgccacatac gccagacctg atccgccaca gtgggcacgg acacgggggg aagaggggcc
     4201 agaggcgtat ctgcggaacg cggagaaggc aaagtcacac ccatttcccc ggtcgggccg
     4261 gccgtcaccg gatcagccca aacggggcct ctcacaggca gaggagtatt gcgcagcaca
     4321 tactgatgca ggcgcggatt atctgcaggc agcagcacca cggcacccgg ctgatctccc
     4381 ctgctgcgga tttccggcac ctccacatgg acaaaaaggc cggcggcaaa agcgcaaacg
     4441 ccaaaaaaaa gcatccacag aaaaatacgc cagcgcaaca ggccggtttc cctccagtca
     4501 aacgtcaatc ccaaatgaag gtcgaaaatt ttatcccggc tctttttatt catcttcatg
     4561 aggtaacaag ggaaaaaatc aatccgcggg ctcggcggca atataacacg tcatcccctt
     4621 ggcaagcacc atatccacca gctgctgttc cagggcccgt gaaacggaat catccaaacg
     4681 cagggcaatc cccatacggc tcccattctc cccatgctcc cgggaaatcc ggtccaattc
     4741 tgaggaaacg ccgggcagcc cgccttcaat caggcggttt ccgatataaa aagcgggctg
     4801 gtcgccagcc gtaactgaaa taaactcaac atcccccaca atattcatca gaaaatggga
     4861 agaaggagcc tttacctcat acccgtacgc cattcccaca cgggaagtca tgatcgtcag
     4921 cacgcagaca agaacaacca aatccagcag gacgaccacc tgaatgaaaa acgccccggc
     4981 gggcaatgtt gaacgggtct tgatctgcat gcctttcctc cttccctgtt gcactcacac
     5041 gccccggcca ttcatgctct ctccggcctc gcgcctctgc cgggcatcac acaccaggca
     5101 aacggcctcc gtgccggccc gttccaggga atgtacaatc tggcgggagc gggaggccag
     5161 gtaggaataa aacaaataag cgggaatagc aatgctcagc cccagggcgg aactaagcag
     5221 ggcttggtaa acggcatccg acatggcaag agagggagcc ttcccttcca gaacccccgg
     5281 ctgggaataa aatcccacca gcccctggat cgtccccagc acgccgacta gcgggctcac
     5341 cgtagccact accagcaatc cgcggatatt cttttcaatc tgaaacactt ccatctgaac
     5401 ggaatcctct gccacagtcc gtagttcttc acgcctcaat ccggcatgcg ccagcacgga
     5461 agaaaccacg cgggccgcag gtcccggtaa aatggaagcc tcatgccgag cttcatcttt
     5521 ccggcattta ccagcttgga aatacctctt aaaaaatctc cagtattaat ttgaatcctg
     5581 tgaaaataca aaatccgctc cgcgacaacc gccagcgcat aaacggccag aacgctcaaa
     5641 acccagaaca acggacctat cttgatgatc aattcattca taaccctctt tctcgcgtta
     5701 gatcatgcac acttcccatg taaattcaac tcaaacctat gtggcccggc agccgagctt
     5761 ctgcagcagc cgcgttcccg cccataataa gacggaaccg agccacagca gagaaccgaa
     5821 actcaggagc cataccgcat caaacaggca catgccggaa agcagcaccg tcacagccct
     5881 ttgaacctgg ggcctgcccg cctcacgccg tgctacggcg gaaatcacgc aggtaaacaa
     5941 aaacaggccg aaagcatacc acagcaaggc cggaggagga acggactctc cacccgaacg
     6001 ggcaaatgcc aagccggcgg ccagcaccca caccccacgg cacaacccca tcaaaacaat
     6061 ggagccgctc cacagtttat ggaacacatt atacagggaa atcacaatca gcagcaaagc
     6121 ccccacggca aattccccgc ccagcgccat gttcagcacc aggccggaca acccggcagc
     6181 caaagacaac agcctcagta cggaaacgct caccgcgccg cacggcacgg ggcggtccgg
     6241 ataacgggcg gcatcccacc tggcgtccac acggtcattc tcccacatgc catacaaata
     6301 aaagcacagc cccatcagca ctacgccccc atacagggcg agaggcggta gctccgcccc
     6361 acggacggaa gcccacgccg ccgccgcgtt ggtaaacagc gtgggaatat tggccggacg
     6421 ggaggaacgg agtactcctc tcaataaacg gcaatcatgc atcgccgcat actgtacagg
     6481 gaaaaacggc gatgaccaag cacatccaca gtcagcaaaa gtgcgggaac cggcatcccg
     6541 cccatgccgg gcagcatcca tacataccca gaaatagatt gtctttgcag gactgtcccc
     6601 tatcatgccg gaggcattaa aaatactctt ctctcccccc atgaatatca ggacccacat
     6661 ccaggcgctt ctcatctgta ccatgcccct gtccgcacag gggcaaagca tcgtggattc
     6721 acgggcctcc cttccacg
//
LOCUS       DAOMSU010000107         6724 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_920_length_6724_cov_3.025191, whole genome shotgun sequence.
ACCESSION   DAOMSU010000107 DAOMSU010000000
VERSION     DAOMSU010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_920_length_6724_cov_3.025191"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="PK077_05865"
     CDS             <1..1679
                     /locus_tag="PK077_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HRN23863.1"
                     /translation="SLQPAERRDFIRDMISDDLASGKHQAPVTRFPPEPNGYLHIGHA
                     KSICLNFGIAQEFPGARCHLRFDDTNPSKEDQEYVDSIQEDIRWLGFDWGDNLFFASN
                     MFDFFYECAVSLIRKGLAYVDEQTVEEIRAQRGNVNVPGQESPYRNRSVEENLARFQA
                     MKNGEIPEGRAILRARIDMASSNMNMRDPVLYRIMFAEHHNTGSSWCIYPMYDFAHPL
                     EDAYEHITHSLCTLEFENHRPLYDWVIENCPVPARPRQTEFARLNLTYTVMSKRKLLQ
                     LVQEGHVSGWDDPRMPTVSGMRRRGYTPAAIRNFCHTIGITKFNGFTDVALLEYSVRE
                     DLNANAPRRMAVLNPLKVTITTLPENAEEMAEVLNNPEKPEEGVRRLPITREVWIERD
                     DFMLDPPKKYFRLAPGRTVRLRGGYCITCTDYKQDAEGTITEIFCEHIPGTIGSNPPE
                     GIQCRAAIHWVSTKYAVDGEIRIYDRLFTEEIPDAAEEGFLSVMNPSSLTVITNAKLE
                     PSLAGAEPEFRCQFERLGYFVADRRDHVPGRRPVFNRTVALKDSWAKKQK"
     gene            1699..2901
                     /locus_tag="PK077_05870"
     CDS             1699..2901
                     /locus_tag="PK077_05870"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HRN23864.1"
                     /translation="MDNQLQFCTVEEAVEEIRQGRMIIVTDDPGRENEADLIIAAEFA
                     TPEAINFMVTHARGLVCAPLSPERADALQLPLMTSVNRENMSTAFTVSVDAAHDITTG
                     ISAAERSLTIRTLADPKATVNDFVQPGHTFPLRAVPGGVLRRAGHTEATIDLVRMAGL
                     QPAGVCCEIMKEDGTMARIGDLGGFQKKHGLKACTVAQLIEYRRAKEKQIRLVETVNM
                     PTDYGDFTCRLYESQLDGALHLALVHGEISPDKPTLVRVHSECLTGDVFGSRRCDCGS
                     QLHTAMRRIAQEGGVLLYLRQEGRGIGLAAKLKAYKLQEQGLDTVEANLKLGYPDDLR
                     DYGIGAQILHDLGANQLRLLTNNPRKIVGLEGFGIKITEQVPLIIPPNEQNSKYLATK
                     KCKLGHIL"
     gene            2921..3409
                     /gene="ribH"
                     /locus_tag="PK077_05875"
     CDS             2921..3409
                     /gene="ribH"
                     /locus_tag="PK077_05875"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HRN23865.1"
                     /translation="MSTELPRRQRATGTRAKICIVASEYNEQYTQALVENCSEELKTV
                     LPSVRLEIIRVPGAFEIPVSIKSVLSRSPEKRPDAVVALGVILRGSTDHADLIGSTIT
                     QALMQLALEFTTPVIHEVLLLNDEKQAFARCIASQLNRGREAARTAARMAELFLNSLL
                     RQ"
     gene            3438..4322
                     /gene="nusB"
                     /locus_tag="PK077_05880"
     CDS             3438..4322
                     /gene="nusB"
                     /locus_tag="PK077_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRN23866.1"
                     /translation="MLSRNQIRQAALQYLYAASQRPETEQEGIWDILMEPFRGDYCKL
                     KAKAVSGHLTRDYPDKLRLFITRARETADKLQQDPLTLPVRDQLQDLLTKEGEFNAAL
                     LRLKKALHEDPANDKGSLSAACDAAQELNTALMQMRRRLLDTLKDFPAYNAIWPSLIS
                     SCRKLQEINDRINCLIHPDDRPSLAEIKKVVEAGRDADELYREAKALGEDILRRRDGL
                     DAAIDSTLENYSPERVSAIDRAILRLGAYELLHRKDLPAPIVISEAIRLSERFSSAES
                     PRFVNGVLAGISKTEHPA"
     gene            4337..5185
                     /gene="ftsY"
                     /locus_tag="PK077_05885"
     CDS             4337..5185
                     /gene="ftsY"
                     /locus_tag="PK077_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRN23867.1"
                     /translation="MAGFFKKLFTKFSRSSRIDWEELEAELVAADIGIRRAMGIADQL
                     KESKGLDAENLVESTREVLRRAFPSTAPSLPVPPEGKPLVILVVGVNGTGKTTSAAKL
                     AHLLQKQGSRVLLAAADTFRAAAVEQLQSWADKLNIPICKGAPGQDPASVCYEAHTQT
                     IREGFQYLICDTAGRLHTRHNLMEELSKIRRTLAKQDASAPHSTLLVVDATTGANALA
                     QAREFHKATPLDSVIITKMDGSGKGGVAVAIMDEMHISPSFLGTGEGAEDFEPFNRDR
                     YVDSLL"
     gene            5204..6283
                     /gene="rlmN"
                     /locus_tag="PK077_05890"
     CDS             5204..6283
                     /gene="rlmN"
                     /locus_tag="PK077_05890"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRN23868.1"
                     /translation="MPPLPLITAQTEEKLLAFLTEHGHTKFRTQQVLDWVWRKRVTTF
                     DAMSNLPPALKNLLAENFRFHTPEIVEIHGSADTTRKFLTRMEDGSLVESVIIPAAAA
                     ENGEKSERVTLCVSSQVGCAFGCKFCASGLLGLKRHLTTGEIIGQILSAEAIAGKRVN
                     NIVFMGMGEPLSNFDNVADALEIITSHRGLEIGARHITISTSGFVPGLKKLAAYPRQI
                     RLAVSLHGATDEVRDQIMPVNKKWPLSQLIPALEEWSRGRNQMPTLEYILIRDINDSP
                     KDASHLVRIAKRLHAKVNLIPYNTVEGLPWKRPSEERCRSFRDAVHKARIPVTMRYEK
                     GHDINAACGQLRLRKEQEKNGWTPR"
     gene            6384..>6724
                     /locus_tag="PK077_05895"
     CDS             6384..>6724
                     /locus_tag="PK077_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HRN23869.1"
                     /translation="METTTTTTAVPPLELTRAPRKTPSLDLRDIPQAPGPEKGVLALM
                     AMDPATYVGQCVTIGMTEDYFYLPAHKLLWRLFQARYNKNEPIDIVSITQALEDMHQL
                     EAVGGSAGLAE"
BASE COUNT         1564 a         2240 c         1719 g         1201 t
ORIGIN      
        1 tgtcattgca gcctgccgaa cgccgagatt tcatccgcga tatgatttct gatgaccttg
       61 cctccggcaa gcatcaggcc ccggtaacac gctttccccc ggaacccaat gggtatcttc
      121 atatcggaca tgccaaatca atctgcctca acttcggcat cgcccaggaa tttccgggtg
      181 cgcgctgcca cctgcgtttt gacgacacca accccagcaa ggaagaccag gaatatgtgg
      241 acagcattca ggaagacatc cgctggctgg gctttgactg gggcgataac cttttctttg
      301 ccagcaacat gttcgacttc ttttacgaat gcgccgtttc cctcatccgc aagggattgg
      361 cctacgtaga cgaacaaacg gtggaggaaa tacgcgccca gcggggcaac gtgaacgtgc
      421 ccggacagga atcaccctac cgcaaccgct ccgtggaaga aaacctggca cgcttccagg
      481 ccatgaaaaa cggagaaatc ccggaaggca gagccatcct gcgagccagg atagacatgg
      541 cctccagcaa catgaacatg cgggaccccg tgttgtaccg catcatgttc gcggaacacc
      601 acaacacggg cagttcctgg tgcatttacc ccatgtatga cttcgcccac ccgctggaag
      661 atgcctacga acacatcacc cattccctct gcacgctgga atttgaaaac caccgccccc
      721 tctatgactg ggtcattgaa aactgccccg tcccggcacg cccgcgccag acggaattcg
      781 cccgcctgaa ccttacctac accgtcatga gcaagcgcaa gctgctgcaa ctggtgcagg
      841 aaggacacgt ctccggctgg gacgatccac ggatgcccac cgtctccggc atgcgccgcc
      901 ggggctacac tcctgccgcc atccgcaact tctgccacac cataggcatc actaaattca
      961 acggcttcac agatgtggcc ctgctggaat acagcgtaag ggaagacctg aatgccaacg
     1021 cgccgcgccg catggccgtg ctcaatcccc tcaaggtcac tatcaccacc ctgccggaaa
     1081 acgcggaaga aatggctgaa gtgctgaaca atccggaaaa accggaagag ggcgtccgcc
     1141 gcctccccat cacaagggaa gtctggattg aacgggacga cttcatgctg gaccctccta
     1201 aaaaatactt ccgtctggcc cccggccgca ccgtacgcct ccgggggggc tactgcatca
     1261 cctgcacgga ctacaagcag gatgcggagg gaactatcac ggaaatcttc tgcgaacaca
     1321 ttcccggcac catcggctcc aaccctccgg aaggaatcca gtgtcgcgcc gccattcact
     1381 gggtaagcac caaatacgcg gtggacggag aaatccgcat ctatgaccgc ctttttacgg
     1441 aagaaatccc ggacgctgct gaagaagggt tcctgtccgt catgaatccc tcatcgctga
     1501 ccgtcatcac aaacgccaag ctggaaccct ccctggccgg agccgaacca gaattccgct
     1561 gccagttcga acgtctcggc tactttgtgg cggaccgcag ggaccatgta ccgggccgtc
     1621 gtcctgtttt caaccgcacg gtggctctca aggactcctg ggcaaaaaaa caaaaataac
     1681 ctctcttcat ccccttttat ggacaatcaa cttcaattct gcaccgttga agaagcagtg
     1741 gaagaaatac ggcagggacg gatgatcatc gttacggacg accctgggcg tgaaaatgag
     1801 gcagacttga tcattgccgc cgaattcgcc acgccggaag ccatcaactt catggttacc
     1861 cacgccaggg gcctcgtctg cgcacctctt tcaccggaac gggcggacgc cctccaactc
     1921 ccgctcatga cctccgtcaa ccgggaaaac atgtccacag cgttcaccgt ctccgtggat
     1981 gcggcccatg acatcactac gggcatcagc gccgcggaac gctccctcac cattcgcacg
     2041 ctggcggacc ccaaagccac cgtcaacgac tttgttcagc cgggtcacac attccctctc
     2101 cgcgccgttc ccggcggagt gctgcgccgc gccggccata cggaagccac cattgacctc
     2161 gtgcgcatgg caggcctcca gcccgccggc gtctgctgtg aaatcatgaa ggaagacggg
     2221 accatggccc gcatcgggga cttgggagga ttccagaaaa aacatggtct gaaagcctgc
     2281 accgtagccc agctcattga ataccgccgg gccaaggaaa agcaaatccg gctggtggaa
     2341 accgtcaaca tgcccacaga ctacggtgac tttacctgcc gcctctatga atcccagctg
     2401 gacggtgctc tccacctggc cctggttcac ggggaaatct ccccggacaa acccacgctg
     2461 gtgcgcgtgc atagtgaatg cctgacgggt gatgtcttcg gctcccgccg ctgcgactgc
     2521 ggcagccagt tgcacaccgc catgcgccgc atcgcccagg aaggaggcgt cctgctctac
     2581 ctgcggcagg aaggccgagg catcggcctg gcggcgaaac tcaaggctta caaacttcag
     2641 gaacagggtc tggataccgt ggaagccaac ctcaagctcg gctatccgga cgacctgcgc
     2701 gactatggca tcggcgcgca aatcctgcat gacctgggag caaaccagtt gcgtctgctt
     2761 accaacaacc cgcggaaaat cgtggggctg gagggctttg gcataaaaat cacggaacag
     2821 gttcctctca tcatcccacc caacgaacaa aacagcaaat acctggctac caaaaaatgc
     2881 aagctgggcc acatcctgta actttccact ccatcccttc atgtccacgg aacttccccg
     2941 ccgccagcgc gccacgggca cgcgcgccaa aatctgcatc gtcgcctcgg aatacaacga
     3001 acaatacacc caggccctgg tagaaaactg ctcggaagaa ctgaaaaccg tgcttccctc
     3061 ggtccgcctg gaaatcatcc gcgtaccggg agcctttgaa attccggtgt ccattaaatc
     3121 cgttctctcc cgctctccgg aaaaacgtcc tgatgccgtg gtggctctgg gagtcatcct
     3181 gcgcggcagc acagaccacg cggacctgat cggctccacc attacccagg ccctgatgca
     3241 gctcgccctg gaattcacca cccccgtcat tcatgaagtc ctgctgctga atgatgaaaa
     3301 gcaggccttc gcccgctgta tcgcctctca gctcaaccgc ggccgtgaag cggcccgcac
     3361 cgctgcccgc atggcggaac tcttcctcaa ctccctgctc cggcaataat acctattact
     3421 ctctcttctc tatcgccatg ctctcacgca accaaatcag acaagccgcc ctccaatacc
     3481 tttacgccgc ctcccagagg ccggaaacgg aacaggaggg aatctgggat atcctgatgg
     3541 aacctttccg gggagactac tgcaaactca aggccaaggc cgtctccggc cacctgacaa
     3601 gggactatcc ggacaagctg cgcctcttca tcacccgtgc gcgagaaacg gcggacaaac
     3661 tgcagcaaga ccctcttacc ctgccggtcc gcgaccagct tcaggacctg ctgaccaagg
     3721 aaggagaatt caacgccgcc ctgctccggc tgaaaaaagc cctgcacgaa gaccccgcca
     3781 atgataaggg ttcgctttcc gccgcctgcg acgccgccca ggaactcaat acagcgctga
     3841 tgcagatgcg ccgccgcctg ctggacacgc tcaaggactt cccagcctac aacgcaatct
     3901 ggccctccct catttcctcc tgcaggaaac tccaggaaat caatgaccgc atcaactgcc
     3961 tcatccaccc ggacgaccgc ccctccctgg cggaaataaa aaaagtggtg gaggcaggac
     4021 gggatgcgga tgaactctac cgggaagcca aagccctggg ggaagacatc ctgcgccgcc
     4081 gggacgggtt ggacgccgcc atcgactcca cgttggaaaa ctactccccg gaacgcgtca
     4141 gcgccattga ccgcgccatc ctgcgcctgg gcgcctatga actccttcat cgcaaagacc
     4201 tcccggctcc catcgtcatc tcggaggcca tccgcctgtc ggaacgcttc tcctcagcgg
     4261 aatccccccg cttcgtcaac ggcgtcctgg ccggaatctc aaaaacggaa cacccggcat
     4321 aactccgctt cgcgccatgg caggattctt taaaaaacta ttcaccaaat tctcccgaag
     4381 ctcccggata gactgggaag aactggaagc tgagctggta gccgcggaca tcggcatcag
     4441 gcgcgccatg ggtattgcgg accagctgaa ggaaagcaaa ggcctggatg cggaaaatct
     4501 ggtggaatcc acccgtgaag tgctccgccg ggcctttcct tccaccgctc cgtccctgcc
     4561 cgtccctccg gaaggcaaac cgctggtcat tctggtcgtg ggcgtcaacg gcaccgggaa
     4621 aactacttct gcggccaaat tggcccattt gctgcaaaaa cagggctccc gcgtccttct
     4681 ggctgcggcg gacacttttc gcgcggccgc cgtggaacag cttcaaagct gggcggacaa
     4741 gctgaatatc cccatctgca agggagcacc cggccaggat ccggcctccg tctgctatga
     4801 ggcccatacg cagaccatca gggaaggctt ccaatacctc atttgcgata ccgcgggacg
     4861 cctccatacg cgccacaacc tcatggagga actctccaaa attcgccgga ctctcgcaaa
     4921 gcaggatgca tccgcccccc acagcaccct gctggtagtg gatgccacta caggcgctaa
     4981 cgccctggca caagctaggg aatttcataa agcaacgccc ctggactccg tcattatcac
     5041 caaaatggac ggttccggaa agggaggcgt ggccgtagcc atcatggatg aaatgcacat
     5101 ttccccctcc tttctgggaa cgggggaagg agccgaagac tttgaaccct tcaaccggga
     5161 ccgctacgtg gattccctgc tgtaacggga ccgcctcaaa tccatgccac cgcttcccct
     5221 catcaccgcc cagacggagg aaaagctgct cgccttcctg acggaacatg ggcacaccaa
     5281 attccgcacc cagcaggttc tggactgggt gtggcgcaag cgcgtcacca cctttgacgc
     5341 gatgagcaac ctcccccccg cgctcaaaaa tctgctggcg gaaaatttcc gtttccacac
     5401 gccggaaatc gtggaaatcc acggctctgc ggacaccaca cggaaattcc tcaccagaat
     5461 ggaagacggc agcctggtag aatccgtcat catccctgcc gcagcagcgg aaaatggaga
     5521 aaaatcggag cgcgtcaccc tttgcgtttc ttcccaggta ggctgtgcct tcggctgcaa
     5581 attctgcgct tccgggctgc tgggccttaa acgccacctc accacggggg aaatcatcgg
     5641 gcagattctc tccgcggaag ccatcgcggg aaaacgggtc aacaacatcg tcttcatggg
     5701 catgggggaa cccctttcca attttgacaa cgtggcggat gccctggaaa tcatcacttc
     5761 ccaccgcggt ttggaaatag gggcgcgcca catcaccatc tccacgtcgg gattcgtgcc
     5821 cggcctgaaa aaactggcgg cctatcccag gcaaatccgt ctggccgttt ccctgcacgg
     5881 agccacggac gaagttcgcg accaaatcat gccggtcaac aaaaaatggc cgctctccca
     5941 gctcattccg gcccttgaag aatggagcag aggaagaaac cagatgccca cgctggaata
     6001 catcctcatc cgggacatca acgactctcc gaaagacgcg tcccatctcg tccggatcgc
     6061 caaacgcctg cacgccaaag tcaatctcat cccgtacaac accgtggagg ggcttccgtg
     6121 gaaacgccct tcggaagaac gctgccgttc cttccgggat gcagttcaca aggcgcgcat
     6181 ccccgttacc atgcggtatg aaaaaggtca tgacatcaac gccgcctgcg ggcagctgag
     6241 gctgagaaaa gaacaggaaa agaacggatg gacaccccgt tgacggaacg gggctttttc
     6301 gaatttgcaa cgttgcctat tgcccggctc cctccgttgt gctatccttc ccggccggat
     6361 gccacccatc cagtcgcctc accatggaaa ctacgacaac caccactgcc gttcccccgc
     6421 tggaactgac cagggccccc cgcaaaaccc cgtccctgga cctcagggac attccgcagg
     6481 ctcccggccc ggaaaaagga gtgctggccc tcatggccat ggacccggcc acttacgtgg
     6541 ggcagtgcgt gaccatcggc atgacggagg actacttcta cctccccgcg cacaagctgc
     6601 tctggcgcct tttccaggcc cgctataaca aaaacgaacc cattgacatc gtttccatca
     6661 cccaggcttt ggaagacatg caccagctgg aagccgtagg cggaagcgcg ggcctggcgg
     6721 aaat
//
LOCUS       DAOMSU010000108         6658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_935_length_6658_cov_2.483417, whole genome shotgun sequence.
ACCESSION   DAOMSU010000108 DAOMSU010000000
VERSION     DAOMSU010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6658
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_935_length_6658_cov_2.483417"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..109
                     /locus_tag="PK077_05900"
     CDS             17..109
                     /locus_tag="PK077_05900"
                     /inference="COORDINATES: protein motif:HMM:NF015446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5 family C-terminal domain-containing
                     protein"
                     /protein_id="HRN23870.1"
                     /translation="MDVIVAESVPESGLGRAIMDRLRRASAVRE"
     gene            165..509
                     /locus_tag="PK077_05905"
     CDS             165..509
                     /locus_tag="PK077_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRN23871.1"
                     /translation="MAATLFEKICSGEIPADIVYQDDLCVCFRDISPQAPEHLLLVPR
                     KPIPRLSEAGEEDAALLGHMMLAVGRIARTLHLDEGGFRLVINNGHDAGEAVPHLHMH
                     LLAGRKLEWPPG"
     gene            620..1111
                     /locus_tag="PK077_05910"
     CDS             620..1111
                     /locus_tag="PK077_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23872.1"
                     /translation="MKRLLVELENLPEAGKKYSGELDSEIFGIDDEEVTSIGPLAFDL
                     HVQRFENELFVRGNISAPFKFRCVRCLGYFDYVVEVCDFATSFQIDSQNVVDVSDSLR
                     EEIVLDFPAYPKCADGGMENDCMAKTPYFGVDKEGGTGVNSSAPIRNSGVWDALNELG
                     DHH"
     gene            1135..1326
                     /gene="rpmF"
                     /locus_tag="PK077_05915"
     CDS             1135..1326
                     /gene="rpmF"
                     /locus_tag="PK077_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRN23873.1"
                     /translation="MAAPKRRTSKMKQRTRLAAQAWRAPKLRKCQNCGSSTPGHTACP
                     NCGTYKTRSGKEIVVKAEA"
     gene            1475..2524
                     /gene="plsX"
                     /locus_tag="PK077_05920"
     CDS             1475..2524
                     /gene="plsX"
                     /locus_tag="PK077_05920"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HRN23874.1"
                     /translation="MKIALDVMGGDNAPDINVDGAKRALQDFPLIEKIYLVGREETVR
                     SSCDRWGLSGPRVEIVPAAEVVEMNESGLLAVRKKKNSSMSISVDLVKSGDADAVVSA
                     GNTGAAVAAATVKLRLLDGVERAGIVTQLPNEFGVCNVTDTGANPDAKPRHLVGYAVM
                     ASILARSVYGKQMPKVGVMSNGSEDEKGTDFTKGTFCLLKHLEERGALPFKFVGNVEG
                     HDLFEHEIDVALTDGFTGNVLLKTCEATAKAFSKWLKEELKANPFRMVGAACASGAFR
                     AMKARLSADSVGGSPLLGVKGVTIIAHGSSTPVAIRNALRVSMEMVQQGVNPLIEEEM
                     ARLGTIPEVAEVYPK"
     gene            2602..4290
                     /locus_tag="PK077_05925"
     CDS             2602..4290
                     /locus_tag="PK077_05925"
                     /inference="COORDINATES: protein motif:HMM:NF019318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRN23875.1"
                     /translation="MSRSAGDRLDSSLAVSVGLCVLVFIVLQWMGSYSPHWGKSIWHH
                     VSEAFLLFSVTLALMPMYWLCAGSLRRKNKTFVVTWGAVLVQLLFFGLIRHVTADITS
                     DIGNELLYLPYMMAPLVVTVLLGPLLGMFATISICMLGGFFILPEQYTPEKQVQFWIL
                     SSLSGMLTVLLTHNLRNRAQLLRAGFFVGLLVMVLCCIMGVINLQAWDYNLTAVLVCL
                     AVAFGVSMLTSVLISGVLPIIEGAFKIITPISWLEMADMNRPLMKRLQMEAPGTFHHC
                     LMVAQLAEAAAEAIGANPIECRVEAYYHDIGKMQNPLYFIENIMDGPNPHDELTPSMS
                     ARIIIDHVQDGVALARENNLPRPLVDVIEQHHGTSLAYFFYRKALQYRDEILSRVESG
                     LASPDDVPEVVESNFRYKGPNPQSKETGIVSLADIVESATRSMGKISCEEMQKRVDEL
                     LKQRVVDGHLDDCGLTFGDLKKIRNSFIKTLKSIHHNRIAYPSHNPEAKIEKNAFSDV
                     SVREKEEKAVKKEGEGKAVPEIMELPDAGTLQEGKSTEAEMENGAVTEKNETES"
     gene            4375..4770
                     /gene="ybeY"
                     /locus_tag="PK077_05930"
     CDS             4375..4770
                     /gene="ybeY"
                     /locus_tag="PK077_05930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRN23876.1"
                     /translation="MLALPEGPVPVLSGLDEVEISVVSDDVIRDVHARFLNDPTATDV
                     ITFPHGDRIGEIIVSFDTAIRQASEFGEPVFRELFRYMVHGLLHLHGYIDTEPEERER
                     MFSCQEPLVRKFGATLVDIPFTHDRCSGA"
     gene            4838..4914
                     /locus_tag="PK077_05935"
     tRNA            4838..4914
                     /locus_tag="PK077_05935"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4872..4874,aa:Arg,seq:tct)
     gene            5045..6109
                     /locus_tag="PK077_05940"
     CDS             5045..6109
                     /locus_tag="PK077_05940"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRN23877.1"
                     /translation="MKNTLVATDYPNLYRSKESEIFYARIDTGRKTVKKSLKTRVLTE
                     ALSRLAGFLAEQGRDELPVESVSWYLAVDMYVQRQEMRPHLKPAAVESIKFFSGRAKK
                     LVARDIAAEAITEQMCRAWWKKDALSVSARTANGTLAVVKNVFSMLQEAGSIKSNPAA
                     KLERMTLRSSNLNVPEKEDFRRIVEEVKKAPILRKWQKKGLYSEAADMIAFLAYSGLR
                     IEEVRRLVWGDIGKESISVPDIKHATSRRTLYINASLAEVIESLRRERRGNSPDDPVF
                     AIESPRKALTNACIRLGLPHVRIHDLRHFFATSCIEAGIDIPTVAKWLGHRDGGALAM
                     KVYGHLRDEHSKEAARKLTF"
     gene            complement(6364..>6658)
                     /locus_tag="PK077_05945"
     CDS             complement(6364..>6658)
                     /locus_tag="PK077_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23878.1"
                     /translation="TLLISLNSNKIRQLENVKSYYNHLIGYMNANLKGALIFVIYSFI
                     LLHMIDSNQIFNLAEHWWKPFAFALWLGCGTYMCTAFWRISSIFTVLLKKKSV"
BASE COUNT         1613 a         1527 c         1826 g         1692 t
ORIGIN      
        1 ggatgaaaat gaagctattg acgtcattgt ggcggaatcc gttccggaat ccggcctggg
       61 ccgcgctatt atggatcgcc tgcgacgcgc ttcagccgtc agggaataac ttgcccacgg
      121 tttgcaaaac atgttaaagt cattgcagat tcctttttga ttttatggcc gctacgttat
      181 ttgaaaaaat ctgttcaggg gaaatccccg cggacatcgt atatcaggat gacctgtgtg
      241 tttgcttccg ggacattagt ccacaagcac cggaacattt gctgcttgtt ccgcgcaagc
      301 ccattccccg cctttccgag gccggggagg aagatgccgc ccttctggga cacatgatgc
      361 tggcagtcgg gcggattgcc cggactcttc atctggatga aggtggcttt cgcctggtca
      421 tcaacaatgg gcatgacgcc ggggaagctg tcccccattt gcacatgcat ctgttggcag
      481 gccgcaagct ggaatggcct cccggttagg ccggagcgaa ttcgggcatt gcctgaaaga
      541 gggacggatg tgtcattttc catgaatggt gccgccctgc ctgcttgtca ggcattaagt
      601 atgcctgtaa gatatactca tgaaaagatt gctggtagaa ctggagaact tgccggaggc
      661 agggaaaaaa tacagcggtg aactggattc tgaaattttc ggcattgatg atgaagaggt
      721 tacttccatt gggcctcttg cttttgatct gcatgtgcag agatttgaaa atgaattgtt
      781 tgtaagaggc aatatttccg ccccctttaa attccgctgt gtgcgttgcc tgggctactt
      841 tgactacgtg gtggaagttt gtgatttcgc gacttctttc cagattgatt cacagaatgt
      901 tgtggatgtt tcagattcct tgagggaaga aatagttctg gattttccgg catacccgaa
      961 gtgtgctgac ggaggcatgg aaaacgactg tatggcgaaa actccgtatt ttggagtgga
     1021 caaagagggg ggaacgggtg taaacagttc tgccccgatc aggaatagcg gcgtgtggga
     1081 cgccctcaat gaactgggag atcatcatta atcacctttt taccaactaa agttatggca
     1141 gcacctaagc gcagaacgtc caagatgaag cagcgtaccc gccttgccgc tcaggcatgg
     1201 cgcgcgccga agctccgcaa gtgccagaat tgcggcagca gcacccccgg ccataccgcc
     1261 tgccccaatt gcggtacgta caagacccgt tccggcaagg aaatcgtggt gaaggcggaa
     1321 gcgtaagttc cgcgggcgcc gcatttgcct tgaaaattat cgccgtattg cggcgctatc
     1381 atatagactt tccagccgct tttgcttcgc aaagcggctg aacttgcttg aagcggactg
     1441 ttttttctgc taacacattt cttcaacaga taatatgaag attgcactgg atgtgatggg
     1501 cggcgacaac gcgcccgata ttaacgtgga cggggcgaag cgggccctgc aggattttcc
     1561 tctcattgaa aaaatttatc ttgtgggaag ggaagaaacg gtgcgcagct cttgtgaccg
     1621 ctgggggctt tccggcccgc gcgtggagat cgttccggct gcggaagttg tggaaatgaa
     1681 tgaatccgga ttgctggcgg tgagaaagaa aaaaaattca tccatgtcca tttccgtgga
     1741 tctggtcaag tccggagatg cggatgccgt ggtcagcgcg ggcaatacgg gggcggctgt
     1801 agcggctgcc accgtcaaac tgaggttgct ggatggtgtg gaacgtgcgg gcattgtgac
     1861 ccagcttccg aatgaattcg gagtctgcaa tgtgactgat accggagcca acccggatgc
     1921 caagccccgg catttggtag ggtatgccgt tatggccagc attctggcgc gttctgttta
     1981 cggcaaacaa atgcctaagg tgggagttat gagcaatggc tctgaagatg aaaagggaac
     2041 agattttacc aagggaactt tttgtctgtt gaagcatttg gaggagcgcg gtgcgctccc
     2101 ttttaaattc gtaggaaatg tggaaggcca cgatcttttt gaacatgaaa tagatgtggc
     2161 tctgacagac ggttttaccg gaaacgtcct gttgaaaaca tgcgaggcta ccgccaaggc
     2221 tttcagcaaa tggctgaaag aggaacttaa ggcaaacccg ttccgtatgg ttggagctgc
     2281 ctgcgcctcc ggagcattcc gtgccatgaa agcgcgcctt tctgcggact ccgtaggggg
     2341 aagccctctt ttgggcgtta agggcgtgac catcatagcg catggcagtt ctacccccgt
     2401 agccatccgc aatgccttga gagtttccat ggaaatggtc cagcagggcg tcaatcccct
     2461 catagaagag gagatggccc ggctgggcac tattccggag gttgccgaag tgtaccccaa
     2521 ataattatca tgtttcattt tctgaaaaag catgccgccg cagcggcaaa agaaagtgcc
     2581 tccccggaag gggctgcctc cgtgtcccgt tcagccggtg accgtttgga ttccagcctg
     2641 gcggtatccg tcgggttgtg cgtgctggtt ttcatcgtgc ttcaatggat gggcagttac
     2701 agtccccact ggggaaaatc catatggcat catgtgtcgg aagcttttct gcttttttcc
     2761 gtgacgctgg cgctgatgcc tatgtactgg ttatgcgccg gttccttgcg caggaaaaac
     2821 aaaacctttg tcgtcacgtg gggggcggtt ctggtccagc tcctgttttt tggtctcatc
     2881 cggcatgtaa cggctgacat tacttccgac ataggcaatg aacttttgta ccttccctat
     2941 atgatggctc cgttggttgt cacggtgctg ctgggacctc ttctgggcat gtttgccact
     3001 atttccatct gcatgcttgg cggatttttc attctgccgg agcaatatac tccggaaaag
     3061 caggtccaat tctggattct gagctctctc tccggcatgc ttaccgtgtt gcttacccat
     3121 aacctgcgga atcgtgctca gttgctgcga gcgggcttct ttgtggggct gcttgtcatg
     3181 gtgctgtgtt gcatcatggg tgtcatcaat ctccaggcat gggactacaa ccttactgca
     3241 gttctggttt gcctggccgt ggcttttggc gtaagcatgc tgaccagcgt gctgatcagc
     3301 ggtgtcctgc ctatcataga aggagctttt aaaatcatta cgcccatttc ctggctggaa
     3361 atggcggata tgaaccgccc tctcatgaaa aggctgcaaa tggaagctcc gggaactttt
     3421 catcattgtc tgatggttgc ccagctggcg gaagctgcgg cggaagccat tggggccaat
     3481 cccattgaat gccgggtgga ggcctactac catgacattg gcaagatgca aaaccccctt
     3541 tatttcattg aaaatatcat ggatggcccc aatccccatg atgagctgac gcccagcatg
     3601 agcgcccgta tcatcatcga ccacgttcag gacggggtgg cattggccag ggagaacaac
     3661 cttccgcgtc ctttggtgga cgttatagag cagcatcacg gcacgtcact ggcttacttc
     3721 ttttaccgga aagcccttca ataccgtgat gagattctga gccgcgtgga aagcggccta
     3781 gcgtctccgg atgacgttcc ggaggtggtg gaatccaact tccgttacaa ggggcccaat
     3841 ccgcagagca aggaaacggg aatcgtcagc ctggcggaca tcgtggaaag cgccacgcgc
     3901 tccatgggta aaatttcctg tgaggaaatg cagaaaaggg tggatgaact tttgaagcaa
     3961 cgggtggtgg acggccattt ggacgattgc ggccttacct tcggagatct caaaaagatt
     4021 cgcaacagtt tcattaagac gctgaaaagc atccaccaca accgcattgc ctatccttcc
     4081 cacaatccgg aggcaaaaat tgaaaaaaat gctttctctg atgtgtccgt cagggaaaaa
     4141 gaagagaaag cggtgaaaaa ggaaggtgaa ggcaaagccg ttcctgaaat catggagttg
     4201 cccgatgccg gaacccttca agagggaaaa tccacggaag cggagatgga gaacggtgcg
     4261 gttacggaga aaaatgaaac cgaatcttga actctacaat catctgggga ggggaggctc
     4321 agttcatccg ttacggatgc cctgattgga gccttggaga gttgtcttcc gtccgtgttg
     4381 gcgttgcctg aaggtcctgt ccccgtactt tccggattgg atgaagtaga aatcagcgtg
     4441 gtttccgatg acgtcatccg ggatgtgcat gcccgttttt tgaatgatcc gacggcgacg
     4501 gatgtcatta cttttccgca tggagacaga ataggagaga tcatcgtcag ctttgatacc
     4561 gctatccggc aggcttcgga attcggagaa cctgttttca gagaactttt ccggtacatg
     4621 gtgcatggtc ttctgcatct gcacggatat atagatacgg aaccggagga acgggaacgc
     4681 atgttttcct gtcaggagcc tctggtgcgg aagtttggag cgaccctggt ggacatacct
     4741 tttacgcatg accgctgctc cggcgcttaa agcgcgcttt tcaaagatcc gggcttgcca
     4801 agttgagatg aactctgtta ctattttgcc gcatcatgcg tccatagctc agttggatag
     4861 agcaactgcc ttctaagcag tgggtcactg gttcgaatcc agttggacgt gccattataa
     4921 accccgtaaa ctgaaaggtt tgcggggttt tcttttcttt tgtctttgtg ttggttgtcc
     4981 gtatggttgt ccgtaaaaag agattattct tgcggtttaa gggccgcaag gcatgcttgg
     5041 aagtatgaaa aacacccttg ttgcgacgga ttatccgaac ttgtatcggt caaaagagtc
     5101 ggaaattttc tatgcccgaa ttgataccgg aagaaaaacg gtgaaaaaat ctcttaaaac
     5161 gcgcgtgttg acggaagccc tttccaggct ggccgggttc ctggcggagc aagggaggga
     5221 tgaattaccc gtggaatccg tgtcttggta tttggcggtt gatatgtacg tccagcggca
     5281 ggaaatgcgc ccccatttga agccggccgc tgtagaatcc atcaagttct tttccggccg
     5341 cgctaaaaag cttgttgctc gtgatattgc agcagaggcc atcacggaac aaatgtgccg
     5401 ggcttggtgg aaaaaagatg cgttgtctgt gtctgcacga acagcaaacg gaacactcgc
     5461 tgttgtgaaa aatgtttttt ccatgcttca ggaagcagga agtatcaaga gcaatccagc
     5521 agccaagctt gaacggatga ctttgaggag ttcaaatctt aacgttccgg aaaaagaaga
     5581 tttccgaaga atcgttgagg aagtgaaaaa agcccctata ttaaggaagt ggcaaaagaa
     5641 ggggctatat tccgaagcgg cggatatgat cgctttcctg gcttattcag ggttacgtat
     5701 tgaggaagtt cggcgcttgg tgtggggaga tatcgggaaa gagtccattt ccgtgcctga
     5761 tatcaaacat gccacctcac gccggaccct gtacattaac gcatctctgg cggaggtgat
     5821 agaaagcctc cgaagagaaa ggcgggggaa tagcccagat gacccggtat ttgccataga
     5881 aagcccccga aaggccctta caaacgcgtg tatcaggctt ggactgcctc acgtccggat
     5941 tcacgattta cggcatttct tcgccacgtc ctgcattgag gcaggcattg atattcctac
     6001 ggtggctaaa tggctagggc atcgtgacgg cggagcattg gctatgaagg tatatggaca
     6061 cctccgagac gaacacagta aggaagcggc taggaaactc actttttagt tatttgcggc
     6121 ttgtaacccg gaagatgtac ccgcctactg gcaaattttc cctgaagacg gagacttcct
     6181 agtttctgcc gggtgattgc agggttatga ttccgaagcc atagctaaag ctatgaaacg
     6241 ccttgctctg taaaaagaga tttttaggag aattattatg aaatcggtac gctaacatat
     6301 tagggcttat caaagataag gacgtttatt tttaagagtt tctgtctggt gtttcaaatg
     6361 aggttaaaca gattttttct ttagaagaac tgtaaaaatg gaactaattc tccaaaatgc
     6421 tgtacacata tacgtaccgc accctaacca tagggcaaag gcaaaaggct tccaccaatg
     6481 ctctgccaaa ttaaatatct ggttactgtc tatcatgtgg agcaatatga aggaataaat
     6541 aacaaaaata agagctcctt ttaaattggc attcatgtaa ccaataagat gattataata
     6601 gctttttacg ttttctagtt gtctaatttt attcgagtta agactaatta ataatgtt
//
LOCUS       DAOMSU010000109         6651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_939_length_6651_cov_3.130685, whole genome shotgun sequence.
ACCESSION   DAOMSU010000109 DAOMSU010000000
VERSION     DAOMSU010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6651
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_939_length_6651_cov_3.130685"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /gene="ppk1"
                     /locus_tag="PK077_05950"
     CDS             complement(<1..1416)
                     /gene="ppk1"
                     /locus_tag="PK077_05950"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRN23879.1"
                     /translation="MSDEYINRELSWLEFNQRVLDQAVRTDLPLLERLKFLAITASNL
                     DEFFMVRVGGLQMLRHSGSRVKDISGLTPTRQLKAIRSRVGRMIEDQYRLFHEEILPW
                     MEINGINPLAMEDLSTSQKLTLEQYFNNQIFPLLTPLDMDAPEAPALPSLKLLMGVEL
                     RDNETGEVRSAVVALPDGLPRRVPVPSAEAERYVMLEDLVRECIGTVFPGETVLSAAV
                     FRVTRNGDIAVQEEDALDLADEMEEVLVARKFSECVRLEVESSAPAPLHDKIMRIVGA
                     GKEETYDLKGPLMLSDFMEMAFAPGNDQLKVEQWSPQPSPSVNPAVSIFENIAGGDIL
                     LNHPYESFEPVLRLVEEAAEDPDVIAIKQVLYRTARNSRIVAALKKAAESGKQVTALV
                     ELKARFDEARNLEKAEELQRSGVQVVYGVKGLKTHAKICLVVRREQGMLRRYCHFGTG
                     NYNETTAKIYTDISYLTCDDQL"
     gene            complement(1452..3014)
                     /locus_tag="PK077_05955"
     CDS             complement(1452..3014)
                     /locus_tag="PK077_05955"
                     /inference="COORDINATES: protein motif:HMM:NF014587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRN23880.1"
                     /translation="MAMIQGEQVQDSELSTQAVIYLGPSSMSLMVAEAVRDRIRLLDF
                     LQQPVPMARDIFRFHRISRHTMDRCVQIIGDYLEILKEYGAGSKLSVRFMISNIISEA
                     DNVDVFVNRMHVAHGLRGRRIDDGKMTRLIYVKVQEALAQYPGFSKKKVLVVHTGPGN
                     TRVLLFQKGRIVRYSCYRLGTHRTGEAVGEIEYGDDVAELSILREHMRGQVDQICLDY
                     GGVKGLAGLIVIGQEMQQLRDRLSPTPEGKVACSSLAAEAERMSRTTLEQRMNVYGAD
                     FAGVDSLLPAVLMTEMIARSLNLDDVIIPASGYDEEFSSSLIRAEQHPGDLEAEVLHF
                     AGILADRYKADKGHREHVARLCMEMFDQLQDLHRLSEHDRLLLEVASILHEVGSFINQ
                     QNHQLHSQYIILNSEIFGLSRDDVETIALLARYHRHEVPANSDPMYGELELRDRMRVA
                     KMAAILRVADALERGHAQRVNGVRAHIRGRMLELELQGVRETAVEDLALRLKGDLFAD
                     IFGYDVVLAPQR"
     gene            complement(3174..4241)
                     /locus_tag="PK077_05960"
     CDS             complement(3174..4241)
                     /locus_tag="PK077_05960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRN23881.1"
                     /translation="MTPPVSSVPVILEKTAARRMVWVDVARVVACILIIANHMAFYSA
                     ELYRWIWAEFLAARTPFFLLAAGYFVGRGSFGPLDGGSLFLWRRSWFLLRPYLVWGLV
                     AAVLIGWSPLHEYYASYRPMYEWLEGEEGAGFWPALAAFGRCLGIAGHPVDAPMWFLR
                     DIIIYTAAAPLLYRLGDYLLWAGMVMLSLNYFALDLADHDYPIANSLGFFLLGMYVSR
                     YSLPFLTEAVRRYAVPFLAATLALTWWLAGHRDQHNSMLIALGMLGLMSLAILFTEWF
                     PRTARWVAGLAPACFFIFGTHYIVIILLRESGWITWKGEAWDMLWLCLVPVIFAVLAG
                     LFFCMKRFAPRLVPLLGAYGK"
     gene            complement(4261..5058)
                     /gene="map"
                     /locus_tag="PK077_05965"
     CDS             complement(4261..5058)
                     /gene="map"
                     /locus_tag="PK077_05965"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRN23882.1"
                     /translation="MAERIHIKSPGEVAKMQKAGAVTAEILMEIGDEVQVGRTTREID
                     DIAREIFKKHKVGNSFYQYRGFPGQLCISINEEVVHGSAGPRRIQNGDIVSLDVGAVV
                     DGWHGDNAMTVPVGMVDPEKLRLLAVTEESLFRAIELVRPGALLADVCAAVEGFVRPR
                     GFTVVRDFVGHGIGRHLHEEPQIPNYRPHYKLPRLKKGMALAIEPMVNAGRPSVKILD
                     DGWTVVTADGKPSAHFEHTVIVTDGAPLIVTDRPRIALPEQLGIAPL"
     gene            complement(5072..5722)
                     /locus_tag="PK077_05970"
     CDS             complement(5072..5722)
                     /locus_tag="PK077_05970"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRN23883.1"
                     /translation="MKELKHFILVGAPASGKGTQGRFLADTFGLHSLSTGSLLRREVE
                     SCTELGRKALSYMDRAMLVPDEIVNDMVRGWLSEMDHGAWLLDGYPRTVAQAETLDHF
                     LNQRGTSVDVVVWMDVSRELIEQRIMRRRECSTCGYVVQTPEEKCSKCGGKMVSRKDD
                     NMEAFARRWKDFEAMTLPVARYYEARGLVVKMTVSEEREPAEVSRDLARKLEEYRQ"
     gene            complement(5751..>6651)
                     /locus_tag="PK077_05975"
     CDS             complement(5751..>6651)
                     /locus_tag="PK077_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HRN23884.1"
                     /translation="SVNIIHALPGAVTLAWKTLVYKDGAVVPMGAMLLVALIAFLIVV
                     VALVVTVTQAQRRIPVQYAKRVMGNKMFNGATQYLPLKLNYSGVMPVIFATAILSLPQ
                     VLFSQIAHYNTTLQWIATKMNDWLNPASSGYYIISGLMIFFFSYFWVATMFQPSQIAE
                     DLKRNGGYIPGIRPGPPTALFLDQTMQRLTFAGSAFLTLIYFLPSLLNIGGNIPYLVT
                     QFFGGTSLLILVGVLLDMMRQVETTLLSRNYDGFLKKGKLKGKYGHIQGTGAAAGSRT
                     VTVMWIVIALLVLAGAIAYNAMQ"
BASE COUNT         1474 a         2043 c         1889 g         1245 t
ORIGIN      
        1 cagctggtcg tcgcaggtga ggtaggaaat atccgtgtaa atcttggcgg tggtttcatt
       61 gtaattgccc gtgccgaaat ggcagtaacg ccgcagcatg ccctgttccc ggcgcaccac
      121 caggcaaatc ttggcatggg tcttcaaccc cttgacgccg taaacaacct gcacgccgga
      181 acgctgaagt tcctccgcct tctccagatt gcgggcttcg tcaaaacggg ctttcagctc
      241 caccagcgcc gtcacctgct tgccgctctc cgccgctttt ttcagggcgg ccacgatgcg
      301 ggagttcctg gcggtacggt acagcacctg cttgatggcg atgacgtccg gatcttccgc
      361 ggcctcctcc accaggcgca ggacgggttc aaaactttca taaggatggt tgagcagaat
      421 atccccgccg gcaatgtttt caaaaatgct gacggccgga ttcacggaag gggacggctg
      481 cggagaccac tgctccacct tcaactggtc attgccgggt gcaaaggcca tttccatgaa
      541 gtcggaaagc atcaggggcc ccttgaggtc ataagtctcc tccttcccgg cgcctacaat
      601 ccgcatgatt ttgtcatgca ggggggcggg cgcggaggac tctacttcca gcctcacgca
      661 ttcggaaaat ttccgggcta cgagcacttc ctccatctca tccgccaaat ccagggcgtc
      721 ttcctcctgc acggcaatat ccccattgcg cgtgacgcgg aacaccgctg cggaaagaac
      781 ggtttcaccg ggaaaaacgg tgccgatgca ctcccggacc aggtcctcca gcatcacgta
      841 gcgctccgcc tccgcggaag ggacgggaac gcgccgcggc aggccgtccg gaagggccac
      901 cacggcggag cgcacctccc cggtctcatt gtcgcgcagt tccacgccca tcagcagctt
      961 gagggacggc agcgcgggag cctccggagc atccatgtcc agcggcgtca gaagcgggaa
     1021 tatctgattg ttgaaatact gctccagcgt cagtttctgg gaagtgctga gatcctccat
     1081 cgccaggggg ttgatcccgt tgatttccat ccacggcaga atttcctcat ggaacaggcg
     1141 gtattggtcc tcaatcatgc ggcccacgcg ggagcggatg gctttcagct ggcgggtggg
     1201 cgtcagcccg gaaatgtcct tcacgcggga accggaatgc cgcagcatct gaagaccgcc
     1261 cacgcgcacc atgaaaaact catccaggtt ggaggcggta atagccagaa acttcaggcg
     1321 ttccaggagg ggaagatccg ttcttacggc ctggtccagc acgcgctggt tgaattccag
     1381 ccaggaaagt tctctgttga tatattcgtc agacatggga aaaatggaaa atggaagttt
     1441 tttaaatcag cctaccgctg gggcgccagc acgacgtcat acccgaaaat atccgcaaac
     1501 aggtcgccct tcagccgcag ggccaggtct tccacggcgg tttcccgcac gccctgaagc
     1561 tccagctcca gcatgcgccc gcggatgtgc gcccggacgc cgttcacgcg ctgggcatgg
     1621 ccgcgttcca gggcgtccgc cacgcgcagg atggcggcca tcttggctac gcgcatgcgg
     1681 tccctcagct ccagctcccc gtacatggga tcggaattgg cgggaacctc atgccggtgg
     1741 tagcgggcca gcagggcgat cgtttccaca tcatcccggg aaaggccgaa aatttcactg
     1801 ttgagaatga tatactggga atggagctgg tggttctgct ggttgatgaa agagccaacc
     1861 tcatgcagga tggaggccac ttccagcagc agccggtcat gctcggaaag gcggtgcagg
     1921 tcctgaagct ggtcaaacat ttccatgcac aggcgcgcca catgctcgcg gtgccctttg
     1981 tccgccttgt acctgtccgc cagaatcccg gcgaaatgaa ggacctccgc ctccagatcc
     2041 cccggatgct gttccgcgcg tatcaggctg cttgaaaact cctcatcata accgcttgcg
     2101 ggaataatga cgtcatccag gttcaggctg cgggcaatca tttccgtcat caagacggcg
     2161 ggcagcaggg aatccacccc ggcgaaatcc gccccgtaaa cgttcatgcg ctgttccaga
     2221 gtggtgcggg acatccgctc cgcctccgcc gccagggagg agcacgccac cttgccttcc
     2281 ggcgtagggg aaaggcggtc ccggagctgc tgcatttcct ggccgatgac gataaggccc
     2341 gccaggccct tcacgccccc gtaatccagg caaatctggt ccacctgtcc gcgcatgtgc
     2401 tcccgcagaa tggaaagctc cgccacatcg tctccgtact cgatttcccc gacggcctcc
     2461 cccgtgcggt gcgttcccag cctgtagcag gaataacgca cgatgcgccc cttctggaac
     2521 agaagcacgc gggtattgcc cggccccgta tggaccacaa gcaccttttt cttgctgaat
     2581 cccggatact gggccagagc ctcctgcacc ttcacgtaga tgaggcgcgt catcttgccg
     2641 tcgtcaatgc ggcgcccccg caggccgtgt gccacgtgca tgcggttcac gaacacgtcc
     2701 acattatccg cctcggaaat gatgttggaa atcatgaacc ggacggaaag cttgctgccg
     2761 gctccgtatt ccttgagaat ttccagatag tcgccgataa tttgcacgca gcggtccatg
     2821 gtatgccggg aaatgcggtg gaaccggaaa atatcacgcg ccatcgggac gggctgctga
     2881 aggaaatcca gcaggcgaat cctgtcccgg acagcctcag ccaccatcag gctcatggag
     2941 ctcgggccca gatagatcac cgcctgagtt gataattccg aatcctgaac ctgctcgcct
     3001 tgaatcattg ccataccatg tattgtttcc cggccgtgga gcaagaacgg caacgtgaca
     3061 aaaaagccgc ttccacccct tccagcaaag gaaagaataa gggatttgcc ttttgacggc
     3121 aagagataag cgcggctccc ttcccgtccc ggcggaacgg ctcttcaaga cggctatttc
     3181 ccataggccc ccagcagggg aaccagccgc ggggcaaatc ttttcatgca gaaaaaaagc
     3241 cccgccagca cggcgaaaat gaccggaacc agacaaagcc agagcatgtc ccacgcttcc
     3301 cccttccatg taatccagcc gctttcacgc aacaggatga tgacgatgta atgcgtcccg
     3361 aaaatgaaaa aacacgccgg agccaacccc gccacccatc tggccgtgcg cggaaaccat
     3421 tccgtgaaca gaatggccag ggacatcagc cccagcatgc ccagggcgat gagcatcgag
     3481 ttgtgctggt cccggtggcc tgccagccac caggtaagcg caagcgtggc agccagaaag
     3541 ggcacagcgt accggcgcac ggcctccgtc aggaacggga gggaataccg ggaaacatac
     3601 attcccaaca ggaaaaatcc caggctgttg gcgatgggat aatcatgatc cgccagatcc
     3661 agcgcaaaat agttcaggga cagcatgacc atgcccgccc agagcagata gtcccccaag
     3721 cggtaaagaa ggggagccgc agcagtataa ataataatat cgcgcaggaa ccacataggc
     3781 gcgtccacgg gatgcccggc aatgcccagg cagcgtccga acgccgcgag agcgggccag
     3841 aacccggcgc cctcctcccc ttccagccat tcatacatag gccggtagga cgcgtaatat
     3901 tcatgcaggg gagaccagcc gatcaggacg gccgcaacca gcccccacac cagatacgga
     3961 cggagaagaa accagctccg cctccagagg aaaaggcttc ctccatccag aggaccgaac
     4021 gagcctctcc ccacaaagta gcccgccgcc agcagaaaaa agggcgtgcg cgccgccaga
     4081 aactccgccc atatccaccg gtacagctcc gccgaataaa aggccatgtg attcgcaata
     4141 atcaggatgc aggcgaccac gcgggccaca tccacccaca ccatccggcg ggcggcggtc
     4201 ttctccagaa tcacgggaac ggaagatacc ggcggcgtca ttccctggag ggggctgaag
     4261 ttacaaagga gcaatgccga gctgttccgg caacgcgatg cgggggcggt ccgtcacaat
     4321 caggggcgcg ccgtccgtta cgataaccgt gtgctcaaag tgggcggaag gtttgccgtc
     4381 cgccgtgacg accgtccagc cgtcatccag aattttaacg gaaggcctgc ccgcgttcac
     4441 catcggctca atagccaggg ccatcccctt tttcaggcgc ggaagcttgt aatgagggcg
     4501 gtaattggga atctggggtt cttcatgcag gtggcggcct atgccgtgcc ccacaaaatc
     4561 ccgaaccacg gtaaaaccgc ggggacgcac gaaaccttcc accgccgcgc agacgtccgc
     4621 cagcaaagcg cccggcctta ccagttcaat ggcgcggaac agggattcct ccgtcacggc
     4681 cagcagccgc agtttttccg gatccaccat acccaccgga acggtcatgg cgttgtctcc
     4741 atgccagccg tccacaacag cgcccacgtc caggctcaca atgtccccgt tctgaatgcg
     4801 gcggggacca gcgctgccgt gcaccacctc ctcattgatg gaaatgcaga gctggccggg
     4861 aaacccgcgg tattggtaaa aggaattgcc taccttgtgc tttttaaaga tttcacgcgc
     4921 gatatcatct atttcccgcg tagtgcgtcc cacctggact tcgtccccga tctccatcaa
     4981 gatttccgcc gttacagccc cggccttctg catcttggct acctcgccgg gagacttgat
     5041 atggattcgt tctgccatgg gattattctt tctattgccg gtactcttcc agcttgcggg
     5101 ccagatcccg ggaaacctcg gcaggttccc gttcttcgga caccgtcatt ttaacgacca
     5161 ggccgcgggc ctcgtagtac ctggctacgg gcagcgtcat ggcttcaaaa tccttccagc
     5221 ggcgggcgaa agcttccata ttgtcatcct tgcgggaaac cattttcccg ccgcacttgg
     5281 agcatttctc ttccggcgtc tgcaccacgt aaccgcatgt ggaacattcc cggcggcgca
     5341 tgatgcgctg ttcaatgagc tccctggaca catccatcca gacgaccacg tccacagagg
     5401 ttccccgctg gttcagaaaa tggtccagcg tttccgcctg cgcaaccgtg cggggatagc
     5461 cgtccagcaa ccacgcgcca tggtccatct ctgacaacca gccgcgcacc atgtcattga
     5521 caatttcgtc cggaaccagc atggccctgt ccatatagct caaggctttc cggcccagtt
     5581 ccgtgcagga ttccacttcc ctccgcaaca gggagccggt gctcagggag tgaaggccga
     5641 aggtatcagc cagaaaccgg ccctgggtcc ctttgccgga tgcgggagcc ccaaccagaa
     5701 tgaaatgctt gagttccttc atatctttac tttcagtaaa aaaggggggc ttactgcatg
     5761 gcgttgtagg cgatggctcc ggccagcaca agcagggcga taacaatcca catgacggta
     5821 acggtgcggc tgcccgcagc ggcgccggta ccctggatgt gcccgtattt gcccttaagt
     5881 ttgccttttt tcaggaaacc gtcgtaattg cgggaaagaa gagtcgtttc cacctggcgc
     5941 atcatgtcaa gcagtacgcc caccagaatg agaaggctgg tgcctccgaa gaactgggtg
     6001 accaggtagg ggatattgcc cccgatattc agcagggaag gcaggaagta aatgagggtc
     6061 aggaatgcgg aaccggcaaa ggtcagcctt tgcatggtct ggtccaggaa aagggcagtg
     6121 ggggggccgg gacggatgcc gggaatatag ccgccgttgc gcttcaggtc ttccgcgatc
     6181 tgggagggct ggaacatggt cgccacccag aagtaggaga agaagaaaat catgagtccg
     6241 gaaataatgt aatagccgga agaagcggga ttcagccagt cattcatctt ggtcgcgatc
     6301 cactgcagcg tagtgttgta gtgggcgatc tgggagaaga ggacctgcgg aagggaaagg
     6361 atggccgtag cgaaaatgac gggcatcacg ccggagtagt taagcttgag aggcaggtac
     6421 tgcgtggcgc cgttgaacat tttattgccc atgacccgtt tggcgtactg cacgggaatg
     6481 cgccgctggg cctgcgtcac cgtcacgacg agcgccacca cgacaatcag gaaggcaatc
     6541 agggccacga gcagcatggc tcccatggga acaaccgcgc cgtccttgta caccaaggtt
     6601 ttccaggcca gggtgactgc ccccggaagg gcgtgaataa tgttgacgga a
//
LOCUS       DAOMSU010000110         6536 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_970_length_6536_cov_2.486808, whole genome shotgun sequence.
ACCESSION   DAOMSU010000110 DAOMSU010000000
VERSION     DAOMSU010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6536
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_970_length_6536_cov_2.486808"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1576)
                     /locus_tag="PK077_05980"
     CDS             complement(<1..1576)
                     /locus_tag="PK077_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRN23885.1"
                     /translation="MVRKLAYLLVILAVLTAVGELCGLLLPTLSWPFTVSREMSMLNI
                     VCTDQNNGGFLTRQGKFLWFGWVCVLVMGGLGFWLMLKGPRRFHPTPITRRRIQRFKS
                     ISRGYVSLLILLVLTLLACMDQCLVGKRALLVVQDGSWYFPAMMRKVYKGSTFGQTGD
                     FADAEANYRELKKQAGQPGKPSLVIMPLVPYDPTGDSTNPGSEALMVNEDGLVCEPGG
                     KPYSGLASRLHKDEEALPHISYKFRKGKKVDRATGWLEDRTEVYSATYENNNIVAEHY
                     SGPGTKEEFLKQTDEHKISRIFYHPSPPLKGGHLLGTNTQGADILAYLYGGLQVNMKA
                     ALFYLPIVYFIGITFGMMMGYFGGMFDLGMQRLIEIFSQVPFLFIIMIISDMVPLHMK
                     GMFLIISLLIMFGWMSMTYQLRTSTMKEKARDYVAAARVLGASTSRILFVHILPNLVA
                     ILVTLVPFSVSALILALASLDYLGFGLPDTYASWGRLLNDGLADLSASWVVTSAFSAL
                     VITLLLVTFIGEAVREA"
     gene            complement(1582..2859)
                     /locus_tag="PK077_05985"
     CDS             complement(1582..2859)
                     /locus_tag="PK077_05985"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN23886.1"
                     /translation="MMQQQSMSALSGQKAGSQRGDNNLSEADMERLEEQYSLQEPIIT
                     AYLQWLGVLPKKIFISREEFGEIGGAGEGDKEDEYSGISDTHTRINLNETGEQVVVVR
                     QDKDSGSVKDAFFSGTPSRKAASEGWTVDIESPMDRAERWARRMRQTDNTEAVRDKAR
                     GYAWRAVMYKTSFDGLLQGTMGNSFKYNEPVWDMIKERIPVSLYFGILSAIITYSVCI
                     PLGVVKAIRHKSLVDNISSVLIFLGYSVPGFALGAVLVVYLGARLEWFPLCGLTSPDF
                     ADMGFWQQAGDLLHHTVLPLICYVVSSFAITTMMMKNNLMDNLSADYIRTAMAKGVSF
                     RGAVIKHAFRNSFIPIASTLGSLISLIVAGSMLVEKVFDIQGFGMLSYQALMDKDYAL
                     IMGTLLLTSFLMVLGNLLSDIIVAAVDPRIKFE"
     gene            complement(3140..3922)
                     /locus_tag="PK077_05990"
     CDS             complement(3140..3922)
                     /locus_tag="PK077_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRN23887.1"
                     /translation="MAKPPQSAMLFAYNTMSRIALISDIHANLPALEAVMKDIEQLQC
                     NAVYCLGDVVGYNANPSECLEFIRSRQIPTVRGNHDEEAIGEDNPAGMNPVAYNALMW
                     TRQQLSEEQKKWLRRAPFQRILPNEIVLVHATQDKPNSWAYVTNVDTATHSINMLRDN
                     QFLCFNGHTHVPRTFIRHEGSIHEYESGDIQLLKTNKYLINVGSVGQPRDGDPRAAYG
                     VLDEDSMVYYQRRVEYDVAEAQRRILAAGLPDMLARRLGEGM"
     gene            complement(4001..4876)
                     /locus_tag="PK077_05995"
     CDS             complement(4001..4876)
                     /locus_tag="PK077_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23888.1"
                     /translation="MLKTIVIFIIALLGFGMIRLPLQNHILEQEKAEGLLDNPVELSS
                     SDYLEQQLAMVSLGGLRSLVAAVLSMEAFDCFLISDWANLERRYNQVTALAPHSDFYW
                     DNGSWHLGNNASSSYLDNKRLSPMERREGFRKYIQKGRRFLEKGINANPDSWYLQSLL
                     GNMYSDTYRQPDFEKAAEAYRKARDLGAPPLTARKEFYALVRVPSKSREALELGRELF
                     KDPRNRTPSLVSNIFVLENRLNIPEKERIPFRELFPTDEIARDLMTSHLANSLKYPVD
                     GLREALESLPPAKDE"
     gene            complement(4896..5813)
                     /locus_tag="PK077_06000"
     CDS             complement(4896..5813)
                     /locus_tag="PK077_06000"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HRN23889.1"
                     /translation="MKIAILGAGALGCYYGARLQESGQDVSFIVRSEYGYLKEHGLQV
                     KSLHGNISLPRINVYRDTEEVGPVDLVVVAWKSTANAGFSRALPPLMGPDTTVVTLQN
                     GMGNAEEIARIIPADRIYVGLCFICAMREKPGHVNHLEGGNIQFAPFIPTPEGTEKAR
                     ELSELFANAGIKTRAFDAAEQIQWYKLVWNIPFNGLCLALGGISIAELYQNPENVVRA
                     RRIMEEVVQAARARGYALPEDLVEFHLSRTETMGAFIPSSAVDYNEGRPVEYTAIWGD
                     PLSKARQAGASVPEWELLDKAIRKRLNMN"
     gene            complement(5841..>6536)
                     /locus_tag="PK077_06005"
     CDS             complement(5841..>6536)
                     /locus_tag="PK077_06005"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRN23890.1"
                     /translation="IVIVATARALKMHGGTALADLKNTDVDALKKGLANLDAHLDAAA
                     HYKRPVVVAVNKFFDDSREELDAIVKHCAERGIPCAIADIFSQGGEGGKDLAQMVVEA
                     ADRSSAPFKPLYESALPVEEKLNIIARNIYGADGVELTAAAKKKLAQFEASRLTDLPI
                     CMAKTQNSLSDNGRLRGRPTGFTITVRDFEIANGAGFLVALCGEIMRMPALPVSPNAM
                     HIYLDDKGNVQGL"
BASE COUNT         1415 a         1726 c         1899 g         1496 t
ORIGIN      
        1 aggcttcgcg gacagcctcc ccgataaagg tgacgagcag gagggtaatc accagggcgg
       61 aaaaggcgga ggtcaccacc cagctggcgg aaagatccgc cagtccgtca ttgagcaggc
      121 gcccccagct ggcgtatgtg tcaggaaggc cgaagcccag atagtccagg gaagccagag
      181 ccagaatgag ggcggaaacg ctgaacggaa ccagagtgac cagaatggcg accaggttgg
      241 gcaggatatg aacgaagaga atacggctgg tggaagcccc aagcacgcgg gcggccgcca
      301 cgtagtcgcg cgctttttct ttcatggtgg aggttctgag ctggtaggtc atggacatcc
      361 acccgaacat gattagaagg ctgataatca ggaacatgcc cttcatatga aggggaacca
      421 tatctgaaat aatcatgatg atgaaaagga agggaacctg ggagaagatt tcaatgaggc
      481 gctgcatgcc gaggtcaaac atgccgccga agtatcccat catcattcca aaggtgatgc
      541 cgatgaaata tacgataggc aggtagaaga gtgcggcttt catatttacc tggaggccgc
      601 cgtacagata agccagaatg tccgccccct gggtattggt gccaagcagg tggcccccct
      661 tgaggggcgg ggaggggtgg tagaagatac ggctgatttt atgttcatcc gtctgtttca
      721 ggaattcctc tttggtgccg gggccgctgt aatgttccgc cacaatgttg ttgttttcat
      781 acgtggcgct gtacacctcc gttctgtctt ccagccagcc ggtggcccgg tccacttttt
      841 tccccttgcg gaatttgtag ctgatgtggg gaagggcttc ttcatcctta tgcaaacggg
      901 aggccaaacc ggaatacggc ttgccgccgg gctcgcagac gaggccgtct tcattcacca
      961 tcaaagcttc cgaaccgggg ttggtggaat ctcccgtggg atcgtatgga accagaggca
     1021 tgatgacaag ggagggtttt cccggctggc ctgcctgttt tttcagttct cggtagttgg
     1081 cttccgcatc cgcaaaatct ccggtctggc caaatgtaga acccttgtat actttgcgca
     1141 tcatggccgg aaagtaccag gagccgtcct gaacgacaag gagggcccgc ttgcccacca
     1201 ggcactggtc catacaggcc aatagcgtca atacaagcaa aatgaggagg gatacgtagc
     1261 cgcgggagat gcttttaaaa cgctggatgc ggcgcctggt gatcggcgtt ggatggaacc
     1321 tccgggggcc tttcagcatg agccagaaac cgaggcctcc catgaccagc acgcaaaccc
     1381 agccgaacca caggaatttc ccctgacgcg tcagaaagcc gccgttgttc tggtccgtac
     1441 agacgatgtt gagcatgctc atttcccgtg agacggtgaa gggccagctt aacgtgggca
     1501 ggagcagccc gcacagctct cccacggcag taagaaccgc cagaatgacc aaaaggtatg
     1561 ccagttttct aaccatgtgt attattcaaa tttgatgcgc ggatccacag cggccacgat
     1621 gatatcggag agaaggttgc ccagcaccat caggaaggag gtaagcagca gcgttcccat
     1681 gatcagggcg taatccttgt ccatcagagc ctggtaactc agcatcccga acccctggat
     1741 atcgaaaacc ttttccacca gcatggagcc ggccacaatc aggctgatga ggctgcccag
     1801 agtggaggca atgggtataa aggagttgcg gaaggcatgc ttgatgacgg cccctctgaa
     1861 actcacgccc ttggccatgg ctgtcctgat gtagtccgcg gaaagattgt ccatcaggtt
     1921 gtttttcatc atcatggtgg tgatggcaaa ggaacttact acataacaaa tcagggggag
     1981 caccgtatgg tgcagcaggt ctccggcctg ttgccagaac cccatgtccg caaagtccgg
     2041 agacgtaagc ccgcagagag ggaaccattc cagccgcgcg cccagataca ccaccagtac
     2101 ggcgcccagg gcgaacccag gcacggagta ccccaggaaa atcaggacgg aagagatatt
     2161 gtccaccagg cttttatggc ggattgcctt tactacccca aggggaatgc agacggaata
     2221 ggtaataatg gcgctcagga tgccgaagta cagggaaacg ggaatgcgct ccttgatcat
     2281 gtcccatacg ggttcattgt acttaaacga attgcccata gttccctgga gcagcccgtc
     2341 aaagctggtc ttgtacatta cggcacgcca ggcatagccc cgagccttgt cgcggacggc
     2401 ctccgtatta tccgtctgcc tcatgcggcg cgcccagcgt tccgcgcggt ccatggggga
     2461 ttctatatcc acggtccagc cttctgaagc ggctttccgg gaaggcgtgc cggaaaagaa
     2521 agcatctttc acgctgccgg aatccttgtc ctggcgaact acgacgactt gttctcctgt
     2581 ttcattcaga ttgatccgtg tatgcgtatc ggaaatgccg gaatattcat cttccttgtc
     2641 tccctctccc gctccgccga tttccccgaa ttcctcacgg gaaatgaaaa tctttttggg
     2701 cagcacgccc agccattgta gataagccgt gatgatgggc tcctgcaggc tgtactgctc
     2761 ttccagccgc tccatatccg cttcactcag gttattgtcg ccgcgctggg agccggcttt
     2821 ctgtccggac agggcgctca tgctttgctg ctgcatcatc tgctcaatgg gacctcccgg
     2881 aacaaaacgg gttatgcaaa agacgagaaa cgtaaccccc aggagcgtca agggcatgag
     2941 aaggagtcgt ctgatgatat aggtcttcat tcgtcgcgct gaactctacc gatttgtgga
     3001 aggtgaggca agtagcaatg caaatttgta agcctttgct aacataaggc gctgcccggt
     3061 tcttcttaag atgaaaggga aacaatgcaa tacggcaggt ttcctttctt ttgacaggcc
     3121 tttcgggagg ggtggactat tacattcctt ctcccaaccg gcgcgccagc atgtccggaa
     3181 gaccggcggc cagaatgcgt ctttgggcct ccgctacatc gtattccacg cggcgctggt
     3241 agtacaccat gctgtcttcg tcaagcacgc cgtaggcggc gcggggatcg ccgtctctgg
     3301 gctggcctac ggagcccaca ttaatcagat atttgttggt tttgagtagc tggatatccc
     3361 cggattcata ttcatggatg cttccttcat ggcggatgaa tgtacggggg acgtgagtgt
     3421 gtccgttaaa gcacaggaat tgattgtcac gcagcatatt gatgctgtgg gtggccgtat
     3481 ccacattcgt gacgtaggcc caggagttgg gcttgtcctg tgtagcgtgc accagaacga
     3541 tttcattggg gagaatgcgc tggaaaggag cgcggcgaag ccactttttc tgttcttcgg
     3601 aaagttgctg tcgcgtccac atcagggcat tgtaagctac tgggttcatc cctgccggat
     3661 tgtcttctcc aatggcttcc tcatcatggt tgccgcgcac ggtgggaatc tggcggctgc
     3721 ggatgaattc aaggcattcg gacggattgg cgttatagcc cacaacatcg cccaggcagt
     3781 aaacggcatt gcattgaagc tgttctatat ccttcatcac cgcttccaga gcgggcagat
     3841 tggcatggat gtcactaatc agggctattc tactcatggt attgtaagca aagagcatag
     3901 cactttgggg aggttttgcc aagcgcgttt gctcacagga aatcatggtt ggggacaggg
     3961 tgccggcctt cccatgaccc cttatttccg tctgtttgat ttactcgtct tttgccggag
     4021 gcaaagattc cagggcttct ctcagcccgt ctaccggata tttcagggaa ttggccagat
     4081 ggctggtcat caggtcacgg gcgatctcat ccgtgggaaa cagctcccgg aaggggatgc
     4141 gttccttttc cggtatgttc agcctgtttt ccagaacaaa aatattactc accaggctgg
     4201 gtgtcctgtt gcggggatcc ttgaataact cccggcccag ttccagagct tctctggatt
     4261 tggaaggaac acggacgagc gcataaaatt ctttgcgggc cgtaagggga ggcgctccca
     4321 aatcccgtgc cttgcggtag gcttccgccg ccttttcaaa gtccggttgc cggtaggtat
     4381 cgctatacat gttgcccagc agggattgaa ggtaccagct gtcaggattg gcgttgatgc
     4441 ctttttccag gaagcggcgc cccttttgaa tgtatttgcg gaagccttcc cggcgttcca
     4501 tgggagaaag acgtttattg tccagataac tggaggaagc gttattgccc aaatgccacg
     4561 atccgttgtc ccagtaaaaa tcggagtgag gagcgagagc ggtaacctgg ttgtagcggc
     4621 gttccaggtt ggcccagtcg gaaatcagga aacagtcgaa agcttccatg ctcagcacgg
     4681 ccgccaccag ggagcggagc cctcccaggg agaccatggc aagttgttgt tccagataat
     4741 cggaagagga aagctctacc ggattatcca gcagcccctc cgctttctcc tgttccagaa
     4801 tatgattctg caaaggcagg cgaatcatgc cgaagcccag cagggcaatg atgaaaatga
     4861 cgatggtctt cagcatgatt cgcaattggt tcgggttaat tcatattcag gcgcttgcgg
     4921 atggccttgt ccagaagttc ccattccggc acggaggctc cggcttgccg ggccttggag
     4981 agagggtctc cccagatagc cgtgtattcg acggggcggc cttcgttgta gtccacggcg
     5041 ctggatggga tgaaagcccc catggtttcc gtacgggaaa ggtggaattc aaccaaatct
     5101 tccggcaggg cgtagccgcg ggccctggct gcctgtacta cttcttccat gatgcggcgc
     5161 gcgcgtacga cgttttccgg attttggtaa agttccgcaa tgctgatgcc gcccagagcc
     5221 aggcaaagcc cgttaaacgg gatgttccag acgagtttgt accactggat ttgctctgcg
     5281 gcgtcaaagg cgcgcgtttt gatgccggca ttggcaaaca gctcggagag ttccctggct
     5341 ttttcagtcc cttccggcgt gggaatgaag ggggcgaact ggatgttgcc cccttccaga
     5401 tggttgacat ggcccggttt ttcccgcatg gcgcagatga agcacaggcc cacatagata
     5461 cggtccgccg ggatgatgcg ggcaatttcc tccgcatttc ccatgccgtt ctggagtgta
     5521 accacagttg tgtccggccc catcaacggg ggaagggctc tagaaaatcc ggcgtttgcc
     5581 gtgcttttcc atgcaacgac aacgaggtct accgggccga cttcctccgt gtcccggtaa
     5641 acgttgatgc ggggcaggga gatattccca tgcaggcttt tcacctgaag gccgtgttct
     5701 ttcagatagc cgtattccga acgtacgatg aaggatacgt cctgccccga ttcctgaagt
     5761 ctggctccgt aatagcaacc cagggctccg gctcccagaa tggcgatttt catggcgtgc
     5821 ggcgcagttg gtgatatttt tcagagtccc tggacgttgc ccttgtcatc cagatagatg
     5881 tgcatggcgt tcggtgagac gggaagggcg ggcatgcgca tgatttcccc gcagagggcc
     5941 accaggaagc cggccccgtt ggcgatttca aagtcgcgca cggtgatggt gaagccggtg
     6001 gggcgtcctc ggaggcgtcc gttgtcggaa agggagttct gggtttttgc catgcagatg
     6061 ggaaggtccg tcaggcggct ggcttcaaac tgggccagct tctttttggc ggcggccgtc
     6121 aattccacac cgtccgcccc gtaaatgttg cgggcaatga tgttgagttt ttcttccacc
     6181 ggcagggcgg attcatagag gggcttgaaa ggggcggaac tcctgtccgc agcttccaca
     6241 accatttggg ccaggtcttt gcctccttct cctccctggg agaagatgtc cgcaatggcg
     6301 cagggaatac cgcgttccgc gcagtgtttc acgatagcgt ccagttcttc ccgggagtcg
     6361 tcaaagaatt tgttgacggc gaccacaacg gggcgtttgt agtgggcggc agcgtccaga
     6421 tgcgcatcca ggttggcaag gccttttttc aacgcgtcca catccgtgtt tttcagatcc
     6481 gcaagggcag ttcccccgtg catttttagc gcgcgtgccg tagctacgat gacaat
//
LOCUS       DAOMSU010000111         6519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_978_length_6519_cov_2.894493, whole genome shotgun sequence.
ACCESSION   DAOMSU010000111 DAOMSU010000000
VERSION     DAOMSU010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6519
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_978_length_6519_cov_2.894493"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..939
                     /locus_tag="PK077_06010"
     CDS             253..939
                     /locus_tag="PK077_06010"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN23891.1"
                     /translation="MRKTLCLLAALALSSLSNAASFSLELSPTGGGAQNTVSLDGITP
                     QDIFGYETDVWHGTITKSALSGQVSLNDGSLGLQLGGVADVGKGNFAGLKFDMPSGVE
                     SAKMSFTITYTSAWGAANFNCDYTCNVYGFTAEGTSTVIGTWSLQNAKASLTQNEPVD
                     VSFDLQLDPDTDYSSFGLIFNNTRVANAGGMGCEISNILISGEAVPEPATASLGLLGL
                     GALLLRRRRN"
     gene            1090..4791
                     /locus_tag="PK077_06015"
     CDS             1090..4791
                     /locus_tag="PK077_06015"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRN23892.1"
                     /translation="MVKVFFKRAAHLLTAFFAGGVCVLQATETSGFGIDLGNGSGTAQ
                     EGWTNVTMPATASGGANTFVAVPLIRNGTASSSSSVAVGSLFGRLVSLTVSARCSSGG
                     SYTLAEPNTRYTWYEGGKHRHNGTNADAPSSFEFPNGCEAFNTSMRLSANGMPSASAA
                     ARLSFSGFQAGKEYTVSFFCGHNAPAYESMTLVSGTLVDVRGLQSSMGSLSGNQFSGL
                     SNSSEQDAYLAVEWRACADEQGRLVFDVTKEADSSASGRMELNAVTVSTDDSPAPEVP
                     DPRPVSVLHNKALIFLPSSLALPVPSSTWAAEGQLTCEFWVRPNDDAVTGKIISLGNA
                     SVSLEHGCLALSVPDGDSVASVKAAGKELAAGEWHHVAVSVGEEVISLYVDGGLAVSS
                     PRSPYLQAMKSGWKGIVLEAGFKGALDELRFWNAALGGEEDDFFLNGPLPLSHPRYGR
                     LVGCWRLDGDFRDAKWTEFAEKTGSAFVRPYQAVAPEGTEFSIVTDNETFRYMLVTAY
                     VRNVHVIYDWPARAHLINNSDLIYINSVTPAADGSLNFQYPDNDVTESSGVVLLPSDG
                     ECSNVLDFSGEGAYMNVGGGLLGGGSSGAFTVEVRMALDGDGQEAVLFENDNVSMRLE
                     WKEDHYRVRTQAGPDKAWEADLPPVEAGRYFWLAFVRNSSADTASFYVDGEAVSTAAA
                     DAGEMEGTANAVIGRNLDGRIEEMRVWHEARAAARLGAAVQHSWGDRLLVGRWGTSDQ
                     FGHDTASWVEHVRILRRLTEGVSGMRIRLGVSGGNWSAMLSDANAREAFAENVAEVVR
                     KHQLDGLDLDFEWIDQNDTAAWNNYGELARAIRAASPDMFFTISLHTYYYKFPAACMR
                     YVDYFTFQNYGPQIDVNGYSSMVSACRTYRSWGYPDSKIMLSAPFQGTPGAGQGADIR
                     AYRDIVSACAGVREDPSLDSASFNYGGGKVKTLHFNGVDTVRKKARYISEQKVAGFMY
                     WDLGMDVADSSGKNNYFDECCLLRAANRYVSSTAYPDTPAPFALSSAGETVPAGGGAV
                     AVEVQSEEKALGWVVADCPDWISASAVSGIGRTTVILTAAENKSADGRFGTVIFRSSD
                     KQECSVIITQDGAELTGYDKWVQDSFPPDAAADRTAADAVPAGDGIPNLMKYATGQDP
                     LKPCGSVTKVTLEEGEDGCMHLVLRWPVNPQATDVKHEVEASTDLVDWISLGEVETAG
                     KTAAEFWDAEPVRESGMERRFLRLKVTRE"
     gene            complement(5089..>6519)
                     /locus_tag="PK077_06020"
     CDS             complement(5089..>6519)
                     /locus_tag="PK077_06020"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23893.1"
                     /translation="QMENPDLMDKPSAEPARTLLNDCLAKAAAGDFITLKGISEYVTP
                     EQWEQLTATLRKKAQGGNARSQADLAYLMLFSYRSEESGYPEALAWARLSAGQGDPCG
                     MYVLGRALIYEFGLEKRMAEGDSWMFRGALKGHVDAADMWSSNNENLQPYSAMMHGLA
                     ARGHIPSLLFLANQAAYPQNTGEAAALDNGFTSACQQVKLTLNGEDANTPPWLRKPEQ
                     KGKGEDIPADAGTVPPWMTPSGRERQAPAPKTINPEAVKFWEQAVELGSLTALDELAN
                     YYETVAGNVQEPAERQQLLASAMTAATALVKKEDVRGFKRLARYYEQGIGVQPNRELY
                     KDYVLKAAETRDPEALVEKARLLIKGEDLEPDPKMALDILTDLEENQTHAVPGMHFLL
                     GYLHEEGLGTPQDMALAYQFYIKGAEQDDAKCMNNLGSMYERGTGVAKNLAEAQKWYE
                     RAAELGNEDALANVERVREKLNTKKK"
BASE COUNT         1286 a         1698 c         1927 g         1608 t
ORIGIN      
        1 aatctgttac catgtttttt cctccagttc cgaacgggaa agaaaacgaa ttgtcccctt
       61 cgcccattat ggaaacacag aatggaagaa aaacggctct tctctccata aaaataattt
      121 ttagattaat tattaacgca ttacatacat gatgaactga aatgccaatg accggattat
      181 tttttgtacc gaaaattttt catcctttgc attcgcaatg cggattctaa atccggtacg
      241 gatttttttg tcatgagaaa aacactttgt cttttagccg ccttggcatt gtcttctcta
      301 tcaaatgctg cctcattttc tttggagctt tctccaaccg ggggcggggc tcagaacacc
      361 gtatcccttg acggaataac accccaggat atctttggtt atgaaaccga tgtctggcat
      421 ggaactataa cgaaaagcgc tttgtcgggc caggtgtcgt taaacgatgg ttcccttggt
      481 cttcaactcg gcggagtagc cgatgtcggc aagggaaatt ttgccggtct caaatttgac
      541 atgccttcag gagtggagtc tgccaagatg tcttttacga ttacttacac ctctgcttgg
      601 ggagccgcca atttcaactg cgattatacc tgcaatgtgt atggattcac ggccgaggga
      661 acatccacgg tcataggaac atggtctttg caaaatgcca aagcttctct tacccagaat
      721 gagcctgttg atgtctcttt cgacctgcag ttggaccctg atacggatta ttcttctttt
      781 ggcttgatat tcaataatac ccgtgtggcc aatgccggcg gcatggggtg tgagatcagc
      841 aacattctta tttccgggga agcagtaccg gaaccggcta cggcctctct gggacttctt
      901 ggcctgggcg cccttcttct gcgccgccgc agaaactgat gacggtcata caggtgattg
      961 accggacagg ctgtgtccaa gccggttttg tgcgaagagg ggatggttca tcccctcttt
     1021 tttatccgtt ttccacgcag ttttttctgg tttgatggac agggacggat aaaatggcgg
     1081 acggtattta tggttaaggt gtttttcaag agggcagccc atttgctgac agcgtttttt
     1141 gcgggaggcg tctgtgtcct gcaggcgacg gagacgtccg gcttcggcat agacctgggt
     1201 aatggttccg gaacggcgca ggagggatgg accaatgtaa ccatgccagc aacggcatcg
     1261 ggaggcgcca atacatttgt tgccgttccg ttaatcagga atggtacggc ttcttcttcg
     1321 tcgtccgtgg cagtgggaag cctgttcgga aggctggttt ccctgaccgt ctccgccaga
     1381 tgcagttctg gaggttctta tacgctggcg gaacccaata cccggtatac ctggtatgaa
     1441 ggggggaagc acaggcacaa cggtacgaac gctgacgcgc cttcttcttt tgaattcccc
     1501 aatggctgcg aagctttcaa taccagcatg cgtctgagcg ccaacgggat gccgtccgcc
     1561 tccgccgcgg ccaggctttc cttctccgga ttccaggcgg gcaaggaata tacggtatct
     1621 ttcttttgcg ggcacaatgc gcctgcttat gaatccatga cgctggtcag cggaactctg
     1681 gtggatgtgc gggggcttca aagttccatg gggagccttt ccgggaatca attttccggt
     1741 ctttccaatt catcggaaca ggacgcttat ctggctgtgg agtggagggc ctgtgcggat
     1801 gaacagggca ggctggtgtt tgatgtgacc aaggaggcgg acagcagcgc ttccggccgc
     1861 atggaactga atgccgttac cgtcagtacg gacgattccc cggctccgga agttccggat
     1921 ccccggcctg tcagcgtctt gcacaataag gcgctcattt ttcttccttc ttctcttgcg
     1981 ctccccgttc cttcttccac atgggcggct gaaggccagt taacctgtga attctgggtc
     2041 aggcccaatg atgatgccgt cacgggaaaa atcatttctt tgggaaatgc gtctgtttcc
     2101 ctggaacacg gttgtctggc tctgtctgtt ccggatgggg attccgtcgc ttccgtgaaa
     2161 gctgccggga aggaattggc tgcgggggaa tggcaccatg tagccgtctc cgtcggggag
     2221 gaggttatca gtctgtatgt ggacggaggc ctggccgttt cctcgccgcg gtcgccttac
     2281 ctgcaggcca tgaaatcagg ctggaaaggg attgtgctgg aggcggggtt caagggtgct
     2341 ctggatgaac tgcgtttctg gaatgcggct ctgggcgggg aggaagacga ctttttcttg
     2401 aacggaccgt tgcccctgtc ccatcccagg tatgggaggc tggtgggctg ctggcgtctg
     2461 gatggggatt tccgtgatgc caagtggacg gaatttgcgg aaaagacagg gagcgccttt
     2521 gtgcgccctt accaggcggt tgcccctgaa gggactgaat tttccattgt cacggataat
     2581 gaaacgttcc gttacatgct ggtgacggcg tatgtgagga atgtgcatgt tatttatgac
     2641 tggccggcgc gcgcccatct catcaataat tccgatttga tctacatcaa ttccgttacg
     2701 ccggccgcgg acggttctct caattttcaa tatccggaca atgacgtgac ggaaagctcc
     2761 ggtgtggttc tgctgccgtc tgacggggaa tgttccaatg tcctggactt ctccggggaa
     2821 ggggcttaca tgaacgtggg cggaggtttg ctgggcggcg gcagttccgg agcgtttacc
     2881 gtggaagtcc gcatggcttt ggacggagac ggacaggagg cggtgctgtt tgaaaatgat
     2941 aacgtttcca tgaggctgga atggaaggaa gaccattaca gggtccgtac ccaggcaggc
     3001 ccggataaag cctgggaggc ggatttgccg cctgtggaag ccggcaggta tttctggctg
     3061 gccttcgtca ggaattcttc tgcggatacg gcgtcctttt atgtggacgg ggaggccgta
     3121 agcacggccg ccgcggatgc gggggaaatg gagggaactg ccaatgccgt cataggaagg
     3181 aatttggatg gcagaattga ggaaatgcgc gtgtggcatg aggcccgtgc ggcggcccgc
     3241 ctgggggcgg ctgtccagca cagctgggga gacaggttgt tggtgggccg ctgggggaca
     3301 agtgaccaat ttggccatga tacagcttcc tgggtggaac atgtccgtat cctccgcagg
     3361 ctgactgaag gcgtttccgg aatgcggata cggctgggtg tctccggcgg gaactggtcc
     3421 gccatgctgt ctgatgcaaa tgcccgtgag gcttttgccg aaaatgtggc ggaagtggta
     3481 cgcaagcatc aattggacgg actggatctg gacttcgaat ggattgacca gaacgatacg
     3541 gccgcctgga ataattacgg agaattggcc agggcaatcc gggcggcttc cccggatatg
     3601 ttttttacca tttccctgca tacctactat tacaaattcc cggcggcctg catgcgttat
     3661 gtggattatt tcacgttcca gaattatggc ccccagattg atgtaaacgg atatagcagc
     3721 atggtttccg catgcagaac ataccgttca tggggctatc cggactccaa gatcatgctc
     3781 agcgctccgt tccaggggac tccgggcgcg gggcagggcg ccgatattcg ggcttaccgg
     3841 gacattgtct cagcctgcgc cggagttcgg gaggatcctt ctctggattc cgccagcttc
     3901 aattatggcg gaggcaaggt taagacgctg cacttcaatg gtgtggatac ggtcaggaaa
     3961 aaggcccgct acatcagtga acagaaagtg gctgggttca tgtactggga tttgggaatg
     4021 gatgtggcgg attcctctgg gaagaacaac tactttgacg aatgctgcct gcttcgggcc
     4081 gccaaccgtt atgtttcctc caccgcttat ccggatactc cggccccctt tgccctttct
     4141 tctgcggggg aaaccgtccc ggccgggggc ggtgccgtgg cggtggaagt gcagtcggaa
     4201 gagaaggctc tgggctgggt ggtcgcagat tgtccggact ggatttccgc ctccgccgtt
     4261 tcgggaatcg gccggactac agtcattttg acggcggcgg aaaacaaatc cgctgacgga
     4321 cggttcggga cggtaatctt ccgttcttcc gacaaacagg aatgctctgt catcataacg
     4381 caggatggcg ccgaattgac gggctacgac aagtgggtgc aggactcctt ccctccggat
     4441 gctgccgcgg accggacggc tgcggatgcc gttcctgccg gggacggtat ccccaacctg
     4501 atgaaatacg ccacaggaca ggatccgttg aaaccctgcg ggagcgttac gaaagtaacg
     4561 ctggaagagg gggaggacgg atgcatgcat ctggtgctgc gctggcctgt aaatccgcag
     4621 gcaacggatg tgaagcatga agtggaagcc tccacggacc tggtcgactg gatttccctg
     4681 ggagaagtgg aaaccgccgg aaagacggct gccgaatttt gggatgcgga acccgtacgg
     4741 gaaagcggga tggaacgccg gtttctgcgg ttgaaagtga ctcgggaata acttaggtac
     4801 gtcctttctg aaaaaaattc cgttcctgct tccgtttttt gagaaatcta tacgatccgg
     4861 cggggcatga ccggctgggg agcgccgcgt gcccataccg caaccggacg ggcggctgtt
     4921 tgtacaagat actcgaatta caaaagcatt gcttcctttg aaacaggcag gggcgcttta
     4981 aaaaatcgcg tccgcttcgc acgcattatt ttctgccgtc tgcagaaacc tgcgctaaaa
     5041 tttgtgttcc accgccattc cgtggcattt ccggaacggg aaaggagcct atttcttctt
     5101 cgtgttgagt ttttccctta cccgttccac attggcaaga gcgtcttcat tccccagctc
     5161 cgccgcccgt tcataccact tttgggcttc ggccaggttc ttggctacgc cagttccgcg
     5221 ttcatacatg gaacccaggt tgttcatgca cttggcgtcg tcctgttccg cccccttgat
     5281 gtaaaactga taggccaggg ccatatcctg tggggtgccg aggccttctt catgcaagta
     5341 gccgagcagg aaatgcatgc cgggtaccgc atgcgtctgg ttctcttcca gatcggtcag
     5401 aatatccagc gccattttcg ggtccggctc cagatcctct cccttgatga gcaggcgcgc
     5461 cttctccacc agagcttccg gatccctggt ttccgcggct ttcagtacat aatccttgta
     5521 cagttccctg ttcggctgca cgccgatgcc ctgctcgtaa tagcgggcca gcctcttgaa
     5581 accgcgtacg tcctcctttt tcaccagggc ggtggctgcc gtcatggcag aggccagaag
     5641 ctgctgccgt tccgcaggct cctgcacatt ccctgccacg gtttcataat aattggccag
     5701 ctcgtccagt gccgtcaggc tgccgagttc cacagcctgt tcccagaact tcacggcttc
     5761 cggattgatg gttttcgggg ccggggcctg gcgttccctg ccggacgggg tcatccaggg
     5821 aggaacggtt ccggcatccg ccggaatatc ttccccctta cccttctgtt ccggtttccg
     5881 gagccacggc ggagtgttgg cgtcctctcc gttcagggtt agcttgacct gctggcaggc
     5941 ggaagtgaat ccgttgtcca gggccgctgc ttcccccgta ttctggggat aggccgcctg
     6001 gttggccagg aaaagcaggg aggggatatg tccccgtgcc gccagaccgt gcatcatggc
     6061 gctgtaaggc tgcaggttct cattgttgga actccacatg tccgccgcgt ccacatgtcc
     6121 tttcaaggct ccccggaaca tccaggaatc cccctcggcc atgcgttttt ccaggccgaa
     6181 ttcataaatg agggcgcggc cgagcacgta catgccgcag ggatctccct gtccggcgga
     6241 aagccgagcc caggccaggg cttccgggta gccggattct tcgctcctgt aactgaataa
     6301 catcaggtat gccagatccg cctggctgcg ggcgttcccc ccctgagctt ttttacggag
     6361 ggtggcggtc agctgttccc attgttccgg cgtgacgtat tcggaaatgc ctttcagcgt
     6421 gatgaaatct cctgcggcgg cttttgccag gcaatcgttc agcagcgttc tggcaggttc
     6481 tgcggacggt ttgtccatta agtccgggtt ttccatttg
//
LOCUS       DAOMSU010000112         6460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_999_length_6460_cov_3.108977, whole genome shotgun sequence.
ACCESSION   DAOMSU010000112 DAOMSU010000000
VERSION     DAOMSU010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6460
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_999_length_6460_cov_3.108977"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..966)
                     /locus_tag="PK077_06025"
     CDS             complement(<1..966)
                     /locus_tag="PK077_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23894.1"
                     /translation="MHNIFRILLLGALPLAAFPVIQASAQEPDIQTLLSAERSSFISG
                     KARDILCRKLGTANLDTDKIRAMAHAPQVALLCHLYQFFSTAENGEPFTQHELKDESF
                     RKWLSTHPEVFRMLALSGAAGKQTLSIFYRIWNANNKTLRPVETSMALGAGLASNVIP
                     PEECLSKFNFYRESYFQSACHPQADTMQPWEWAIVFRGRESLEDLSWAQQFIEKKQIP
                     PEQAGNKFMGFIPYRRKNLQGVSVHAGAAFYDHKPVTLKLYTEYGGVCGAVSKGAAGF
                     LRAKGVPAWAIGQPGHCAFIWKHPGGHWKIGNNISGWNWSTGKSQI"
     gene            complement(1148..1819)
                     /locus_tag="PK077_06030"
     CDS             complement(1148..1819)
                     /locus_tag="PK077_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRN23895.1"
                     /translation="MNLPDAHTHSSTAASGTAPRFICGTSPADWKQVALLAERDEHVT
                     PFFGIHPWFLNPAEWKEEMPRLESLLQKIPHAGVGETGLDKCRRGIPGLPLQKEALER
                     HLELAVRLDRPASLHCCRAWGTLAEMLKKYPRLNIVLHGWTGTLHPGTEFPSGNWLLS
                     VGLREMERPGLLASIPLHRLALESDGHPETLPELYRRAAQELGMTVNNLVDLVQSNME
                     NLIRP"
     gene            complement(1826..3550)
                     /locus_tag="PK077_06035"
     CDS             complement(1826..3550)
                     /locus_tag="PK077_06035"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRN23896.1"
                     /translation="MVLSLLASGGPNDAPPFFILLTVVFMGIIAIALILAHFRQSLLA
                     GYFICGVILANCGILDHIPNSDVSIQALSEVGIILLMFTLGLEFSVDELKHLRKTALV
                     GGGIQMFICTAAFGLGLHYATGMDMSHSLTVGAIMGLSSTAVALKSFQEFGLSGTSGA
                     KMAVGIALFQDIAAIMLVAIMPQIFTATPDSWETALNIGMAVLRGLVFLGAAWLIGRY
                     LLPRIMLAVARTKSRELFTLMVFAICSGIAMLASLLGLSIALGAFTAGLFVSSSYYSH
                     RVLSEVLPFKDLFLTIFFVSAGLLIDIDGLWEHIGQVATFASFVLAIKLACLTAASFL
                     KIPGRMGIMASTALTNVGEFSLVLIPFMQEISPIPALVTTNVYAIAAVSMGMTPLLMK
                     AARKLTPLLVRIPGLKTKRERLNVETLITRMEGIRDHAIICGYGPVGRRLHESLSQYG
                     IPCIIIDLNADTVKSLLNGGHLAFLGDIQHQITMDLAGVRTARLIAFTFPDPSPALST
                     YMQIKGANADITVIARAKFRSEVASLHHAGIANVIHDEMETGAAAVRLAKQSFDIIEP
                     SDAPSLSH"
     gene            complement(3569..4420)
                     /gene="yaeI"
                     /locus_tag="PK077_06040"
     CDS             complement(3569..4420)
                     /gene="yaeI"
                     /locus_tag="PK077_06040"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008460.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HRN23897.1"
                     /translation="MTTHPFSKKRSQLRKFLYAAAAVILLGSLLASYMHWEANHLEFN
                     ENTAPAGCVPGLSGLRILVLSDIHTNLPLLEKAATMAEQARPDMIVFLGDLYTDFLRV
                     THAGDYIAQMKRLSSVAPAYACLGNHDMALADNVERVLKEGGFTLLRNSAAFVSIPRL
                     GNVEFKLVGLGDLREGDFFPDRCMSPRELEENSPMPTIVLSHNPKGRELLGNYRWDLM
                     LSGHTHGGQIKLPFFSTPLLASEGETMHSGFHPYEDKQVFVTRGIGYIGPGRFNCPPE
                     INLITIP"
     gene            complement(4417..5526)
                     /locus_tag="PK077_06045"
     CDS             complement(4417..5526)
                     /locus_tag="PK077_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRN23898.1"
                     /translation="MKDPRFQALAAQLAGYSTALRPGDRVLLELTDIPEEMGIALIRE
                     ARNAGAMPFLRLNQSRLNREMLSGATEEQYGIIGRHRLAEMEDMDAYIEIRGGGNAFE
                     LSSVPQENMAAAMKALNPVSQRRIRHTRWCGLRWPSGGMAQQASMSTEEFEDFYFRTC
                     LMDYAALRPAMRKLAAMMEKAEYVKITGPGTDISFSIKGLPAIPCAGECNLPDGEVFT
                     APVIDSANGHISFNTPSLFQGIPFDNIRLTLKNGLVVHAEAGDKTGELNTILDTDPGA
                     RRLGEFAFGVNPAITRPMRNILLDEKISGSFHLTPGQAYHVADNGNQSRIHWDMVCIQ
                     TKAAGGGDIYLDGKLVRRNGLFTLPELAILNPSLS"
     gene            5608..6195
                     /gene="purN"
                     /locus_tag="PK077_06050"
     CDS             5608..6195
                     /gene="purN"
                     /locus_tag="PK077_06050"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HRN23899.1"
                     /translation="MSRLPKLGILGSGSGSNCQSIYDAIQSGSLRAEIAVVMSDNPDA
                     YILERARSWSIPAEVIDCGGFKTRFPEESQASVAARLKQYGVDCVCLAGFMRLVKLPL
                     LKEFPSRILNIHPSLLPAFPGLHAWEQAVNAGAAESGCTVHYVDDGMDTGPILGQARV
                     PVLPGDTPESLHARIQEQEHTLYPAMIARVLETLA"
BASE COUNT         1335 a         1729 c         2001 g         1395 t
ORIGIN      
        1 tatctggctt tttcccgttg accagttcca tccgctgatg ttgttgccga ttttccagtg
       61 tccgccgggg tgtttccaga taaaggcgca gtgtcccggc tgtccgatgg cccacgcggg
      121 gacgcctttg gctctcagaa agcctgccgc tcctttggag acggctccgc acacgcctcc
      181 gtattccgta taaagcttta atgtgaccgg tttgtggtcg taaaaagcgg ctccggcatg
      241 aacgctgact ccctgaaggt ttttcctgcg ataggggatg aaccccatga atttatttcc
      301 agcctgttcg ggagggatct gttttttctc tatgaactgc tgggcccagg acaggtcttc
      361 caggctctct ctgccacgga aaacgatggc ccattcccac ggctgcatcg tgtccgcctg
      421 gggatggcat gcggattgga agtaggattc ccggtaaaaa ttgaatttgg acaggcattc
      481 ttcaggaggg atgacatttg aggcaagccc tgcccccaga gccatgctgg tttcaacggg
      541 gcggagcgtt ttattgttcg cgttccagat gcggtaaaat atggataggg tttgtttgcc
      601 ggctgccccg gatagggcaa gcattctgaa gacttccggg tgggtggaga gccatttcct
      661 gaatgactcg tccttgagtt catgctgcgt gaacggttct ccgttttccg cggtgctgaa
      721 aaattggtac aggtggcata acagggcgac ttgcggggcg tgcgccatgg cccggatttt
      781 atccgtgtca aggtttgcgg ttcccagttt cctgcacaga atatcccggg cttttccgga
      841 gatgaacgag gaacgttcgg ctgaaagtag tgtctggata tccggttcct gcgctgacgc
      901 ctggataacg ggaaaggctg ccagggggag cgctcctaaa aggaggattc ggaagatatt
      961 atgcatggga gatgggcgga attgtatcca tacctaatat aatccatcat atggcaaaaa
     1021 gatgatgaat tgtcaacgga aatgtttttg ttttctggga aaatgattga acgaagagtc
     1081 aatggaaata gaaacccagt gaatgaaaag gcgccgcgaa accatgtccg cccgttgaat
     1141 ggaggcgtca agggcggatc aggttttcca tattgctttg gaccaggtca accaggttgt
     1201 tgacggtcat gcccagttcc tgcgccgcgc gtcggtaaag ttccggaagg gtttccggat
     1261 gcccgtccga ttccagcgcc agccgatgga gcggtatgga ggcaaggagt cccggacgtt
     1321 ccatttcccg cagccctacg gaaagaagcc agtttcccga aggaaattcc gtgccgggat
     1381 gcaatgtccc cgtccagccg tgcaggacaa tgttcaaacg gggatatttt ttcagcattt
     1441 ccgccagcgt gccccatgcg cggcagcagt gcagggaagc ggggcggtcc agacgcacgg
     1501 ccagttccag atgccgttcc agcgcttcct tctggagagg cagccccggg atgccgcgcc
     1561 ggcatttgtc cagccccgtt tccccaacgc ccgcatgggg gattttttgc agaagggatt
     1621 ccagcctggg catttcctct ttccattcag ccgggttcag gaaccaggga tgaatgccga
     1681 aaaagggggt aacatgttca tcccgttccg ccagaagggc cacctgtttc cagtccgccg
     1741 gggaggtgcc gcagatgaac cgcggcgccg tgccggaagc ggctgtagag gaatgggtgt
     1801 gggcgtccgg cagattcatc gttgcttaat gggacagaga tggcgcgtcc gacggttcaa
     1861 taatatcaaa actctgcttt gccaggcgca cggcggcggc gcccgtttcc atttcatcat
     1921 ggatgacgtt ggcgatgccc gcgtgatgca gggaagccac ttccgaacgg aatttggcgc
     1981 gggcgatcac cgtgatgtcc gcattggcgc ccttgatctg catgtaggtg gacagggccg
     2041 gggaagggtc cgggaatgta aaggcaatca gccgcgcggt acggacgccc gccaggtcca
     2101 tggtgatttg gtgctggatg tctcccagaa aggccagatg gccgccgttg agcagggatt
     2161 tgacggtgtc cgcattcagg tcgataatga tgcaggggat gccgtactgg gacaggcttt
     2221 catgaagcct cctgcctacg gggccgtagc cgcaaatgat ggcatggtcc ctgatgcctt
     2281 ccatgcgtgt gatcagggtt tccacgttga ggcgttcccg ttttgttttc aacccgggaa
     2341 tgcgcaccag cagcggggtg agtttccgtg ccgccttcat cagcagcgga gtcataccca
     2401 tggagacggc ggcaatggcg taaacgttgg tggtcaccag cgccgggatg ggggatattt
     2461 cctgcatgaa agggatgagc accagggaaa attcccccac attggtgagt gcggtggagg
     2521 ccatgatgcc catgcgcccg ggaattttca ggaagcttgc ggctgtaagg caggctaatt
     2581 tgatcgcgag gacaaaggag gcgaaggtcg ccacctggcc gatgtgttcc catagtccat
     2641 caatgtcaat caacagcccg gcggaaacga agaagatggt caggaaaagg tccttgaagg
     2701 ggagcacttc gctaagaacc cggtggctgt agtaggagct gctgacgaag aggccggcgg
     2761 tgaaagcgcc cagggcgatg ctcaggccca gcagactggc gagcatggca attcccgagc
     2821 agatggcgaa caccatcagg gtgaagagtt cccggctttt ggtccgggcg acggccagca
     2881 tgatgcgcgg aagcaggtag cggccgatca gccaggcggc tcccagaaag acaagccccc
     2941 gcagtacggc catgccgatg ttgagcgctg tttcccatga gtcgggcgtg gcggtgaaga
     3001 tctgcggcat gattgccacc agcatgatgg cggcgatgtc ctggaacagg gcaattccca
     3061 ccgccatttt tgccccggag gttccgctca ggccgaattc ctggaaggat ttcagcgcca
     3121 ccgccgtgga ggatagtccc ataatggccc cgaccgtcag ggagtggctc atgtccatgc
     3181 ctgtggcgta atgaagcccc aggccgaaag ctgcggtaca gatgaacatc tgaatgcctc
     3241 cgccgaccag agcggttttc cgcaggtgct tgagttcatc gacggaaaat tcaagcccca
     3301 gcgtgaacat gagcaggata attcctacct cggaaagagc ctggatgctg acgtcggaat
     3361 tggggatgtg gtccagaatg ccgcagttgg ccagaattac cccgcagatg aagtagcccg
     3421 ccagcaggga ctgccggaag tgggccagaa tgagggcaat cgcgatgatg cccatgaaga
     3481 cgacagtaag cagaatgaag aatggaggcg cgtcattagg gcctccggag gccagcagag
     3541 agagaaccat ggcggtcagg tcgggaagtt agggaatggt aatgaggttg atttccggcg
     3601 ggcagttgaa gcggcccggc cctatatagc ctatgccgcg ggtgacgaat acctgcttgt
     3661 cctcataagg atggaacccg gagtgcatgg tttccccttc cgacgccaat agcggggtgg
     3721 aaaagaaagg cagtttgatt tggcctccat gcgtatggcc ggagagcatc aggtcccagc
     3781 ggtaatttcc cagcaattcc cttcctttgg ggttgtggct caggacgatg gttggcatgg
     3841 gcgagttttc ctccaattcg cgcgggctca tgcaccggtc cgggaaaaag tccccctccc
     3901 gcaggtctcc cagccccacc agtttgaatt ccacgtttcc cagcctgggg atggagacga
     3961 aggcggcgga attcctgagc agcgtgaagc ccccttcctt caggacgcgt tccacattgt
     4021 ccgcaagggc catgtcatgg ttgcccagac atgcgtaggc cggggcgacg gaggagaggc
     4081 gtttcatctg ggcgatgtag tcgcctgcat gagtgacccg cagaaaatcc gtgtacagat
     4141 ctcccagaaa cacgatcatg tccggtctgg cctgttccgc cattgttgcc gctttttcca
     4201 gcaggggcag attggtgtgg atatccgaca ataccaggat acgaaggccg gaaaggccgg
     4261 gtacgcatcc ggcaggggcc gtgttttcat taaattccag atgattggct tcccagtgca
     4321 tgtaggaggc cagcagggat cccagcagaa tgacagccgc ggccgcatac aggaacttgc
     4381 ggagctggga acgctttttg gagaaagggt gggtggtcat gatagagagg ggttgagaat
     4441 ggccagttcc ggcagggtga ataatccgtt gcggcgcacc agcttaccgt ccaggtaaat
     4501 atctccgccc ccggccgctt tcgtctggat gcataccatg tcccagtgga tgcgggactg
     4561 gttgccgttg tccgccacgt ggtaggcctg ccccggcgtc aagtggaagg agccggaaat
     4621 tttttcgtca agcaggatgt tgcgcatggg gcgtgtgatg gccggattga cgccgaaggc
     4681 gaattccccc agcctccgcg cgccggggtc tgtgtcaaga atggtgttga gctcccctgt
     4741 tttatctccc gcttccgcat gcacgaccag tccgtttttc agcgtcaggc ggatgttgtc
     4801 aaaggggatg ccctggaaca ggctgggcgt attgaaggaa atgtggccgt tggcgctgtc
     4861 gatgacgggg gcggtgaaca cttctccgtc gggcagattg cattcccccg cgcacgggat
     4921 ggcaggcagc cccttgatgg aaaaggaaat gtccgttccc ggtccggtaa ttttcacata
     4981 ttcagccttt tccatcatgg cggccagctt ccgcatggcg ggacgcaggg cggcgtaatc
     5041 catcaggcag gtgcggaaat agaaatcctc aaactcttcc gtgctcatgg atgcctgctg
     5101 ggccattccc ccggacggcc agcgcagccc gcaccagcgc gtatggcgga tgcgccgctg
     5161 ggagacgggg ttcagggctt tcatggcggc ggccatattt tcctgtggaa cggaggacag
     5221 ttcaaaggcg ttccccccgc cgcgtatctc aatgtaggcg tccatgtcct ccatttccgc
     5281 caggcggtgg cggccgatga tgccgtattg ttcttccgtt gctcctgaaa gcatttcccg
     5341 gttcaggcgg gactggttca ggcgcagaaa gggcattgct cccgcattcc tcgcctcccg
     5401 gatgagggct attcccattt cctccggaat atccgtcagt tccagcagaa ccctgtctcc
     5461 ggggcgcagg gctgtggaat accctgcaag ctgggctgca agggcctgaa aacgggggtc
     5521 tttcatgtct ttattaggca aatacggcag cctttttgtc aagccggaga cttgccacgg
     5581 gcaaaaaact gtggttttat cccccggatg tccagattgc ccaagttagg aatactcggt
     5641 tccggttccg gttccaattg ccagtccatt tatgatgcca tccagtccgg ttctctccgg
     5701 gcggaaatcg ccgtggtgat gtctgacaat ccggacgcct acattctgga acgcgcccgt
     5761 tcctggagca ttccggcgga ggttattgac tgcgggggat ttaaaaccag atttccggag
     5821 gaatcccagg catccgttgc cgcgcgcctg aagcaatacg gcgtggactg cgtgtgcctg
     5881 gcgggattca tgcgcctggt gaaacttccg ctgctgaagg aatttccttc ccgcatcctg
     5941 aatatccacc cttcccttct tccagccttc ccggggcttc atgcctggga gcaggctgtg
     6001 aacgccggag ctgcggagag cggctgcacc gtccattatg tggatgacgg aatggatacc
     6061 ggccccatcc tgggccaggc tcgcgtgccc gtgctgccgg gggatacgcc ggaaagcctt
     6121 cacgcccgca ttcaggagca ggagcatacc ctttatcccg ccatgatcgc ccgcgttctg
     6181 gaaacgctgg catgacctga ttctccttct ggccggaaag caatccggcc tcctgccggg
     6241 agaggatgac aggatgaccg attctcttgt cttgtgatat gaacggaatc cgttaaaaag
     6301 gagtcctctt ttcaaagaaa ggataaaaat accatgaaga tgaacccttc catcgccctg
     6361 tttgccgctt ccgctcttct ggcgggtgtg gcaggcgcgc aagaaaaggc tgccgaagct
     6421 ccggcggccg accaacccaa acaagagaaa gaaattactg
//
LOCUS       DAOMSU010000113         6338 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1025_length_6338_cov_2.883973, whole genome shotgun sequence.
ACCESSION   DAOMSU010000113 DAOMSU010000000
VERSION     DAOMSU010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6338)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6338
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1025_length_6338_cov_2.883973"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="PK077_06055"
     CDS             complement(<1..832)
                     /locus_tag="PK077_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HRN23900.1"
                     /translation="MLNNLTTKFQEALMQAQQTAGRLGKPEISSLDVLVALLEQEGGI
                     LSPILRKASCDPGLLLQAAQREVSHEPTQSGATTHPQMGRDLATVMQAAEEERKKLKD
                     DYLSVEHFMLGALDTQNKVHQLTDTFGLTKDNYLAAMKEVRGNQRVTDDNPEGKYQTL
                     EKYGTDLTARARAGKIDPVIGRDTEIRRVLQILSRRTKNNPVLIGEPGVGKTAIAEGL
                     ARRIVNGDVPESMRNKRIVSLNIGSMLAGAKYRGEFEERLKSFLKEVTDSNGEIILFI
                     D"
     gene            1384..2289
                     /locus_tag="PK077_06060"
     CDS             1384..2289
                     /locus_tag="PK077_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HRN23901.1"
                     /translation="MLTDAVTSDASQIYELYGIDIKPKWFPLLFVLAGGKEKTITGIA
                     KEIGHSHPSVSNIVGEMTARGLVKGLEDQKDKRRTVIALSPHGKRVAAMLTELCKDVE
                     VAVEDISKETRHDLWRAIGEWEDRLAEKSLLQRVKEARKMREREHIRVIPYEPCYKNT
                     FKSLNVQWITQHWKIEPHDLDYLDHPQEYIINKGGFIFVALYRGKPVGVCALCKMDDP
                     VYDYELAKLAVHPHAQGKGIGEILCHAAIDKAKDMKAKILFLESNTLLKPAIHTYKKL
                     GFKELAENHPAYERGNIQMELKITY"
     gene            2291..2644
                     /locus_tag="PK077_06065"
     CDS             2291..2644
                     /locus_tag="PK077_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRN23902.1"
                     /translation="MEIIKNNKVIVRKEDASKKVFKGVSLDSLAVGEKSMAAKMYYVK
                     GNFASTHQHPHEQCGYVISGKYRLNMEMPEKNIEVLLHAGDSYAIPDNTPRSFEVMGS
                     GEVVDVFTPPREDYL"
     gene            complement(2918..3493)
                     /gene="kdpC"
                     /locus_tag="PK077_06070"
     CDS             complement(2918..3493)
                     /gene="kdpC"
                     /locus_tag="PK077_06070"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF001454.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HRN23903.1"
                     /translation="MKWTFPNLRLFLSLAVITGGIYPLVVTLVSLIFFPHQAHGSLIR
                     NERGEIIASELVAQPFISAKYFWPRPSAGEYATMPSGASNLSWTSGKLVRTVAERRAA
                     LLKAHSLPEKTPVPADMLFASGSGLEPFISPEAAEFQLNRVASTRGVPPEKIRKLVSE
                     HFVRGGILGENVVNVMTLNAALDTLFPMSGM"
     gene            complement(3522..5630)
                     /gene="kdpB"
                     /locus_tag="PK077_06075"
     CDS             complement(3522..5630)
                     /gene="kdpB"
                     /locus_tag="PK077_06075"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRN23904.1"
                     /translation="MKEKKSQSWYDGAMLKTAFLNALRKFDPRVLSKNFVMFITAAGA
                     LMATIALIRDLAAGQPFGFTLQIALWLWFTVLFANFAEALAEGRGKAYADALKEMRVY
                     VNARLLTDKGTEEIVSISELRKGDKVLCEAGDIIPLDGEVIEGIASVDESAITGESAP
                     VIRESGGDRSAVTGGTRVISDGIVIRITVEPGHTFLDRMISMVEGASRQKTPNETALG
                     ILLVALTIVFLAVVLSLPAMAGYIEDSARTLGFRAEGRISLPILVSLLVCLIPTTIGG
                     LLSAVGISGIDRLVRRNVLAVSGRAVEVAGDIDVLMLDKTGTITFGNRMANEFLPAPG
                     VDDLQLAEAAQLASLSDETPEGRSIVVLAKKQFGIRGRHMDADHIAFIPFSASTRMSG
                     VDIAEHDGHPAVSIRKGAAESVAQWVKIRGGTFPQEVEMMVQNVARVGGTPLVVARGK
                     DVLGVIYLKDIVKGGIRERFARLRAMGIRTVMVTGDNALTAASIAAEAGVDDFMAEAT
                     PEMKLARIREEQAAGRLVAMTGDGTNDAPALAQADVGVAMNTGTQAAREAGNMVDLDS
                     NPTKLIEIVEIGKQMLITRGALTTFSFANDVAKYFAIIPAMFAGLFVADGMASGPLSA
                     LNIMGLNSPQSAILSTVIFNALVIVALIPLALKGVSYRPAGAASLLKRNILIYGVGGL
                     IAPFMGIKLIDMVINAWRLV"
     gene            complement(5665..>6338)
                     /locus_tag="PK077_06080"
     CDS             complement(5665..>6338)
                     /locus_tag="PK077_06080"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRN23905.1"
                     /translation="SNGSVNCMHSSMSPLGGGIALFNMLLGEVIFGGLGCGLYGMLMF
                     AMITVFLCGLMVGRTPEFLGKKMGAREVCCSMVGILLPGMAVLVMSGLAAATETGRAS
                     ICNAGPHGLTEILYCFGSQAGNNGSAFAGLAAGDTPFYSLLGGLAMLLARFGTIISVM
                     MIAGSMASRKTAPPAQGTMGTDNLMFMVLLVAVVLVVGALTFFPALALGPILEHLLLY
                     SGTML"
BASE COUNT         1594 a         1745 c         1670 g         1329 t
ORIGIN      
        1 catcgataaa cagaatgatt tcaccgttgg aatccgttac ttccttaagg aaggatttca
       61 agcgttcttc aaattcaccg cgatatttgg ctccggcaag catggagccg atgttgaggg
      121 atacaatgcg cttgttgcgc atgctttccg gcacgtcgcc gttgacaatg cgccgcgcca
      181 gaccttccgc aatggcggtt ttgccaacgc cgggctcccc gatcaggacg ggattgtttt
      241 tcgtcctgcg ggaaagaatc tgcaaaacgc ggcggatttc cgtatcacgg ccgatgacgg
      301 ggtcaatttt accggcgcgg gcgcgggccg tcaggtccgt cccatatttt tccagcgtct
      361 ggtatttgcc ttccgggttg tcgtccgtca cgcgctggtt gccgcgcact tccttcatgg
      421 cggccagata gttatccttg gtcagcccaa aggtgtccgt caactggtgc actttgtttt
      481 gcgtgtccaa cgccccaagc atgaagtgtt ccacgctgag gtagtcgtct ttgagctttt
      541 tgcgttcctc ttccgcagcc tgcatgacgg tagccaggtc acgccccatc tgcggatggg
      601 tggtggcgcc gctttgagta ggttcgtggg aaacctccct ttgggctgcc tggagaagca
      661 gccccggatc gcaagaggct ttgcggagaa taggggaaag aattccgcct tcctgctcca
      721 gtaacgccac cagaacgtcc agagaagaaa tttccggttt tccaagccgg cctgcagttt
      781 gctgcgcctg catcagcgct tcctgaaatt ttgttgttag attgttaagc atggtatgtt
      841 ggtattttag acataagaat aactccaacc gttgaatgga aaaacaggat ttttcctgtt
      901 atctaaaaat tttttgcccc tccggattcc ccgcaatatc acttgcacgc tcaacggatt
      961 attggagagt ccgccctgac aaggaaatcc tgtcccgctt tctcatggag ctggcgcatc
     1021 cggtatcccg gcaatagtgt aaatcctgaa cgccaaggaa aatatttccg gaaagaacgg
     1081 cagccccgta ccggatagaa acgcaggaac agcccttgcc catgctccaa aataatcgcg
     1141 tgggcaaggg cggcaatgcc aaataccacg tccgcggcct acccgactga cagaagacgg
     1201 atttttccat tccattgatg gatacagcga tattccccac ctgcaagaag actaaaatgt
     1261 gaaaaaatgc ttgctcgcat gataatggca tattagttac gtaatcagtt acgtatttaa
     1321 taacggaaag atatggattt tttcaatagg acaggtaaaa tagccattgg aagcaggctg
     1381 cgcatgctga cggatgctgt gacgtctgat gcaagtcaaa tttatgaatt atacggcatt
     1441 gacataaagc caaagtggtt tcccctgctt ttcgttttag ccggcgggaa agagaaaaca
     1501 ataacgggca tagctaagga aatagggcac agtcatccat ccgtgagcaa tatcgttgga
     1561 gaaatgacgg caagaggatt ggtgaaaggc cttgaagacc agaaggacaa gagacgcacc
     1621 gtcatcgccc tgtctccaca tgggaaaagg gttgccgcca tgctgacaga gctctgcaaa
     1681 gacgtggagg tcgccgtaga ggatatttcc aaggaaacgc gccatgattt gtggagagct
     1741 atcggcgaat gggaagaccg gctcgctgag aagagcctgc tgcaacgggt caaagaagcc
     1801 cggaaaatga gggaaaggga gcatatcagg gtaattcctt acgaaccgtg ttacaagaat
     1861 acgtttaaat cacttaacgt acagtggatt acgcaacatt ggaagataga accgcacgat
     1921 ctggactatc ttgatcatcc gcaggaatat attatcaaca aaggaggctt tatctttgta
     1981 gccctttaca ggggaaagcc cgtcggagtt tgtgccctct gcaaaatgga tgacccggta
     2041 tatgattatg aactggcgaa acttgccgtt caccctcatg cgcagggcaa aggcatcggg
     2101 gaaatacttt gccatgcagc tatagacaaa gcaaaggaca tgaaggcgaa aattcttttt
     2161 cttgaaagca acaccctgct caaacccgcc atacacacat ataaaaagct gggattcaag
     2221 gaacttgcgg aaaatcaccc ggcatacgaa cgcggaaaca tccagatgga actgaagata
     2281 acgtattgat atggaaatca taaaaaataa taaagtcatc gttcgcaagg aagacgcatc
     2341 gaagaaagtg ttcaagggcg tttcactgga ttcgctcgct gtcggagaga aatcaatggc
     2401 agcaaagatg tactatgtga aaggcaactt cgcctctacc caccaacatc cgcatgaaca
     2461 atgcgggtac gtcatttcag gcaaataccg cctgaacatg gaaatgccgg agaagaatat
     2521 tgaagtgttg cttcatgcag gggacagcta cgccataccg gacaatacgc ctcgttcctt
     2581 tgaggtgatg ggaagcggag aggttgtcga tgtgttcaca ccgccaaggg aggactattt
     2641 gtaaatagcc gcatttgggg ctgaagaaac ttcatatcag cctaataaaa ctaacgagag
     2701 gatgtcgccc cccctattgc tgcattctga aaggattatc cggcagcata ccgcacgccg
     2761 gccaatttat tgtctctgtt tttcaacaaa ttaggccaaa ctgacaaaaa tccaataaga
     2821 aaaccgcccc ggtaaccgga gaaagacgcg gaaccaaggc accggctgat gccggacgat
     2881 ttttccgcca ggatattttc cgaaccgggg cggtcaatta catcccgctc atcgggaaca
     2941 atgtatccag cgcagcgttc agggtcatca cgttgactac gttttccccc aaaattccgc
     3001 cccggacgaa gtgttcagac accagtttcc ggattttttc cggaggcact ccgcgcgtgg
     3061 aggcaacacg gttcaattga aattccgccg cttccggaga gataaaaggc tccaaaccgc
     3121 tgccggaagc aaaaagcatg tccgcaggga caggcgtttt ctccggcaga ctgtgggctt
     3181 tcagcagagc cgccctgcgc tccgccaccg tcctgaccag cttcccggaa gtccaggaga
     3241 ggttgctggc tccggaaggc atggtagcgt attctccggc agagggccgg ggccagaaat
     3301 atttggcgga gataaaaggc tgtgcgacaa gctccgaagc gataatttcc ccccgttcat
     3361 tccggattag ggaaccatga gcctgatgag ggaaaaaaat caggctgacc agcgtaacga
     3421 ccaaagggta aatgcctccg gtaatgacgg ccagagacag aaacaggcgc agattgggaa
     3481 atgtccactt catgataatt cagcaggtta ggattggatt ttcaaacaag gcgccatgca
     3541 ttgatgacca tatcaataag cttgatgccc atgaaaggag caatcaggcc gcccaccccg
     3601 taaatgagga tattgcgctt cagcagggag gccgctcccg ccggacggta ggaaacccct
     3661 ttcaaggcca gaggaatcag cgctacgatg accagggcat taaaaatcac agtgctgaga
     3721 atggcgcttt gcggggaatt caggcccatt atgttcaggg cggaaagagg tccactggcc
     3781 atgccgtcag ccacaaagag gccggcaaac atggcgggaa tgatggcaaa gtatttggct
     3841 acgtcattgg cgaaggaaaa ggtggtcagc gcgccgcggg taatcagcat ttgcttgccg
     3901 atttctacga tttcaatcag cttggtcgga ttgctgtcca ggtccaccat gttgcccgcc
     3961 tcccgggcgg cctgcgttcc ggtgttcatc gccacaccca catcggcctg ggccagggcg
     4021 ggagcgtcat tagttccatc cccggtcatg gccaccaggc ggccggcagc ctgttcctca
     4081 cggatgcgcg ccagcttcat ctcaggcgtc gcttccgcca tgaagtcatc cacgccggct
     4141 tccgccgcaa tggatgcggc agtcagggca ttgtcccctg tcaccatgac agtccggatg
     4201 cccatggccc ggagccgggc aaagcgttcc ctgatgccgc ccttgacgat gtctttcaga
     4261 taaatgaccc ccagcacgtc ttttccccga gccactacca gcggcgtacc gccgacacgc
     4321 gcgacattct gcaccatcat ttccacctct tgcgggaaag ttccgccccg gattttcacc
     4381 cactgcgcca cgctttccgc cgctcctttc cggatggaaa cggccggatg tccgtcatgc
     4441 tccgcaatat ccacgccact catgcgggtg gaagcggaaa acgggataaa agcaatatgg
     4501 tccgcgtcca tatggcggcc ccggattcca aactgttttt tagccagcac cacgatgctg
     4561 cggccttccg gcgtttcatc ggacagggaa gcaagctggg cagcttccgc cagctgcagg
     4621 tcatccacac cgggtgcagg caggaattcg ttcgccattc gattgccgaa ggtgatggtt
     4681 ccggttttat ccagcatcag cacatcaatg tccccggcta cttccacggc gcgcccggac
     4741 acagccagca cattccggcg cacaagccgg tcaatgccgc tgataccaac agcacttaac
     4801 aatccgccga tcgtcgtcgg aatcaggcat accagaagag agaccaggat gggcagggaa
     4861 atacggccct ccgccctgaa gcctagcgtc cgggcagaat cctcaatgta tccggccatg
     4921 gcgggaaggg aaagaaccac ggcaagaaaa acaatggtca gcgccaccag caagataccc
     4981 agagcggttt cattgggcgt tttctgccgg ctggccccct ccaccatgga aatcatgcgg
     5041 tccaggaacg tatggcccgg ttccacggta atacggataa caatgccgtc actgattaca
     5101 cgggttcctc ccgttacagc gctgcggtcc cccccgcttt cacggatgac cggagcggat
     5161 tccccggtaa tggcggattc atccacgcta gcgatgcctt ctatcacctc tccatccagg
     5221 gggatgatat ccccggcctc gcacaacact ttgtcccctt tgcgcaattc ggaaatggag
     5281 acgatttcct ccgttccctt gtccgtcagc aaacgggcgt tcacataaac gcgcatttct
     5341 ttcagggcgt ccgcatacgc ctttccccgg ccttccgcca gcgcttccgc aaagttggcg
     5401 aagagcacgg tgaaccagag ccagagcgcg atttgcaaag taaagccgaa gggttgcccg
     5461 gcagccaaat ccctgataag ggcaatcgtt gccatcaggg cgccggcagc cgtgataaac
     5521 atcacgaagt ttttagacag gacgcgcggg tcaaatttgc gcagcgcatt caggaaagct
     5581 gttttcaaca tggctccgtc ataccaggat tgactctttt tctccttcat gatcataaac
     5641 atccggatta tcggcgaata cgccttaaag catggttccc gaatacaaga gcaaatgctc
     5701 cagaataggt cccaaagcca gggccgggaa gaaggtgagg gctcctacaa ccagcacgac
     5761 ggctaccagc agaaccatga acatcaggtt atccgtcccc atggtgccct gggccggcgg
     5821 agcggttttc ctggatgcca tgctgccagc aatcatcatc accgaaataa tggttccgaa
     5881 gcgggccagc agcatcgcca acccgcccag cagggaataa aaaggcgtat cccctgcagc
     5941 cagaccagca aaggcgcttc cattattccc ggcctgggaa ccgaagcagt acaggatttc
     6001 cgtcagcccg tgggggccgg cattgcagat ggatgcccgc ccggtttccg tagcggcggc
     6061 caggccgctc atgaccagga cggccatgcc gggcagaagg atgcccacca tggagcagca
     6121 cacttcccgg gctcccattt ttttacccag gaattccggg gttcgcccca ccattaaacc
     6181 gcacaggaac acggtaatca tggcaaacat cagcatgccg tacaagccgc agcccagacc
     6241 tccgaaaatt acctcaccca gcaacatatt gaacagggca atgccgccgc ccagcgggga
     6301 catgctggaa tgcatgcagt tcacggaccc gttggaag
//
LOCUS       DAOMSU010000114         6294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1036_length_6294_cov_2.822087, whole genome shotgun sequence.
ACCESSION   DAOMSU010000114 DAOMSU010000000
VERSION     DAOMSU010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1036_length_6294_cov_2.822087"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="PK077_06085"
     CDS             <1..931
                     /locus_tag="PK077_06085"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRN23906.1"
                     /translation="EGKAFHSEWISLGLKAADLKEKQASAGHITDVGEAQVLLHFPAG
                     WKPQDKRTALCIFPGGGYSIQAIEKEGCRIAGWAAEHGMVGVVVKYRVSGTNNAVGRF
                     PGPLLDARQALRTVRENASSLGIDPLRIGVMGFSAGGHLAAMASTLWNRTLPEEAENP
                     LKNISSRPDFSMLIYPVISMAPHTTHGGTRNKILGPHPSPALEEMCSAERQVTKQTPP
                     VFLVHALDDGVSCANSRLMEQACRNKGVPVSLNLYPTGGHGYGMEKRGHPTDQWPDAA
                     EQWLREQGFLPSPASVHGGAPSGAVPSSGTRRE"
     gene            1004..3733
                     /gene="ileS"
                     /locus_tag="PK077_06090"
     CDS             1004..3733
                     /gene="ileS"
                     /locus_tag="PK077_06090"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRN23907.1"
                     /translation="MSAPEKNYKDTILLPETGFPMRGDLTKNEPVRLKKWEDTGLYER
                     ILSRRMAQGAPRFLLHDGPPFANGDVHMGTALNKILKDLVLKSKTMAGYAAPYVPGWD
                     CHGLPIEFKVVQKARDLDAAEIRRRCAEFAKGFIDIQRTSFRRLGVFGDWENPYLTMK
                     PAYEANILRVFAKLVEDGAVYQSRKPVQWSYGAYTALAEAEIDYKEKVSTSVFVRFPL
                     LDNPLGLKASMVIWTTTPWTLPANVGLALHPRFTYVAGRFMKNGQTENLVIVKELLDA
                     FAQKTGWALAETIREFKGAELENCEARHPFLSRTSTIILADFVTTDTGTGIVHIAPGH
                     GADDYNVGRQYGLPVLSPVDDDGKYTEEVGVPSLVGKHVFDANKDIISMLEQGNNLFG
                     VEEYRHQYPHCWRSKTPIIFRAVEQFFISMDKLRPQALEEIDKVQWLPSWGRNRIYGT
                     VEARPDWCISRQRTWGVPLPVFFDENGKAVLDAALVRKIADMVEKHGSNIWFELSDED
                     LCERLGLPKTWKKGKDTLDVWIDSGSSHMAVMDNRPGLDAPADLYLEATDQHRGWFQS
                     SLMLSVAWRGKAPYKAVMTHGFVVDKDTGKKTSKSDAKSGKPIDAAYYYDKYGADIVR
                     LWAASVDWQNEVPFGEDLFKQVTEPYRRLRNTLRILLGNISGFDFAAQAVAPEHMPLL
                     DRWILERLNAVIRETLKAYEAYDFRKAFSAINQFCTSDLSALYVDTTKDRLYCDSSSS
                     VRRRATQTAMTIIFQALCRLLAPILAFTADEAWEYAGYEGSVHEQDFPAPMPEYDTQE
                     ATSVISRLQEIKSVIQVAIEEQVKAKVFSKNNEASITLSVPVNESEDVVALLEDRAFA
                     TEFFIIADLDVKTGPELAASDAKTEHAMCPRCRRYEPAAEGRDVCERCAEVLS"
     gene            3764..4537
                     /locus_tag="PK077_06095"
     CDS             3764..4537
                     /locus_tag="PK077_06095"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRN23908.1"
                     /translation="MDPQTEKEPAPHKKTPPGWLWWSLLGAGLYALDQASKLWIIRRF
                     PLNYLNEKLHKYAYLPDFPGFQHINADGTLDFATNGRMPEAELARLPDLNPNTVDALN
                     KLRELEPISFLDGTMNITRVHNTGVAFGLGNGTVWSSYLFLAVPVLAIVVLVVLYRKN
                     FFHTAWLKLAYVLLLAGVAGNLTDRLIQGFLIPYEQQHGFFTKLMNGYVVDFIDVTIP
                     LFNYRWPAFNVADSCIFVAAIIFFIASIFSARNKEEKPS"
     gene            4545..5207
                     /locus_tag="PK077_06100"
     CDS             4545..5207
                     /locus_tag="PK077_06100"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRN23909.1"
                     /translation="MTIRLAILSGLLLCGSLASGKNELYFKETVVPVKVAPDQDSITA
                     SFPFTNSSGTPVTISKIHVSCDCTTAGAKDNKLTYAPGESGVISAVMKTGNFSGTVDK
                     DMTVHANGSAYKLVIRAQIPDIIRMEPRKLEWARGEAAAPKTIKITISKELPVNLTTV
                     DLTGDAFDYEPVTVKKGREYKIIVTPKSTARPAFNTIWVRTDSAVPRYKRQMGFLAIK
                     EN"
     gene            5225..5707
                     /locus_tag="PK077_06105"
     CDS             5225..5707
                     /locus_tag="PK077_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRN23910.1"
                     /translation="MRDAFSTALKLLLAVFLLAVGVAALDLRVIQPFRTPPCNPETLE
                     EGHVCLSQILKEWPGKILWIDARKQDDFERHTVTQAPVYPIRPADANYQELLANAMEA
                     LMTAEDRGFCIVIFCSRDCTSSAAVANELKKPEYGIRAPIFILEGGWDELRKEPSLVP
                     "
     gene            5710..>6294
                     /locus_tag="PK077_06110"
     CDS             5710..>6294
                     /locus_tag="PK077_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRN23911.1"
                     /translation="MLDTATIRPQFPILETSVHGKPLIYLDNAATTQKPLAVLDAIRH
                     YYDTENANVHRGSHYLSQLATEAHEESRKTVARFINAPETAEVLFTSGCTMGINLAAD
                     TIAGSGMVKPGDEVIVTASEHHSNIVPWQMLCERTGAVLKAVPLTPGQTLDMEAYRNM
                     LSPRTRIVAVGHVSNTLGTVNPVREMTALAKANRQ"
BASE COUNT         1400 a         1997 c         1733 g         1164 t
ORIGIN      
        1 agagggaaaa gcctttcact ccgaatggat ttccctgggg ctcaaagcgg cggacctgaa
       61 agaaaaacaa gcctccgccg gacacattac ggacgtgggg gaagcgcagg ttctgcttca
      121 ctttcccgcc ggatggaagc cgcaggacaa acgcacggcc ctgtgtatct ttccgggagg
      181 ggggtattcc atccaggcca ttgaaaagga agggtgccgc attgccggct gggcggcgga
      241 acacggcatg gtgggggttg tcgtgaaata ccgcgtttca ggaaccaaca atgccgtggg
      301 gaggtttccc ggtcccctgc tggacgcccg ccaggccctg aggaccgtgc gggaaaacgc
      361 ctcctccctg ggaattgacc ctctccgcat cggcgtcatg ggcttttccg caggcggcca
      421 tctggcggcc atggcttcta cgctgtggaa ccgcaccctt ccggaagaag cggaaaatcc
      481 gctgaaaaac atttcctccc gtccggattt ctccatgctc atctatcccg tcatttccat
      541 ggcgccccat acaacccacg ggggaacgcg caacaaaata ttgggccccc atccctcccc
      601 tgccctggag gagatgtgtt ccgcggagcg gcaagtgacg aaacagacgc ctccggtatt
      661 cctcgtccac gctctggatg acggcgtttc ctgcgccaac agcaggttga tggaacaggc
      721 ctgccggaat aagggggttc ccgtcagcct taatctctat ccaacgggag gccacgggta
      781 tggcatggaa aaacgcgggc atcccacgga tcaatggccg gatgccgccg aacaatggct
      841 cagggaacag gggtttcttc cttccccggc ttctgtccac gggggcgctc cttccggcgc
      901 cgtcccctcc tccggaacac ggcgggaatg aatgcgctag gaacccaggt gtgccgcacc
      961 gcagggttga ccggaaccgg gtccagtggt actttttccg ggcatgagtg cgcccgaaaa
     1021 gaattataaa gacaccatcc tgttaccgga aaccggcttc cccatgagag gggatctgac
     1081 gaaaaacgaa cccgtacgcc tgaaaaagtg ggaagacacc gggctgtacg aacgcatttt
     1141 gtcccgcagg atggcccagg gcgcaccccg gttcctcctt catgacggcc cccccttcgc
     1201 caacggggat gtgcacatgg gcaccgccct gaacaaaatt ctcaaggatc tggtcctgaa
     1261 atccaaaacc atggcaggct acgccgcccc ctatgttccg ggctgggact gccacggcct
     1321 tcccattgaa ttcaaggtgg ttcagaaggc ccgtgacctg gatgcggctg aaatccgccg
     1381 gcgctgtgcg gaattcgcca aaggattcat tgacattcag cgcacttcct tccgccgcct
     1441 gggcgtgttc ggcgactggg agaatcccta cctgaccatg aagccggcgt atgaggccaa
     1501 catcctgcgc gtgttcgcca aactggtgga agacggagcg gtgtaccaga gccgcaaacc
     1561 cgtccagtgg tcctatgggg cctatacggc gctggcggaa gcggaaatag actacaagga
     1621 aaaggtcagc acctccgttt tcgtgcgctt cccgcttctg gacaatcccc tggggctgaa
     1681 agcctccatg gtcatctgga ccactactcc gtggacgctg cccgccaacg tagggctcgc
     1741 ccttcatccg cgtttcacct acgtagcagg cagattcatg aagaacggcc agacggaaaa
     1801 cctcgtcatc gtcaaggagt tgctggatgc cttcgctcag aaaacgggct gggctcttgc
     1861 ggaaaccatc cgtgaattca agggagccga actggaaaac tgcgaagccc ggcatccctt
     1921 cctttcccgc acgtcaacaa tcatcctggc ggatttcgtc accacggaca ccggaaccgg
     1981 catcgtccac atcgcgcccg gccacggcgc ggacgactat aacgtgggac gccaatacgg
     2041 attgcccgtc ctctcccccg tggacgacga cggcaaatac acggaggaag tgggcgtgcc
     2101 ctccctggtg ggcaaacatg tctttgacgc caacaaggac atcatttcca tgctggaaca
     2161 gggcaacaac ctcttcggcg tggaagaata ccgccaccag tacccgcact gctggcgttc
     2221 caagacgcct ataattttcc gagccgtgga acagttcttc atctccatgg acaagctccg
     2281 cccgcaggcc ctggaagaaa tagacaaggt gcagtggctg ccctcctggg gccgcaaccg
     2341 catttacggc acggtggaag ccaggccgga ctggtgcatc tcccgccagc gcacctgggg
     2401 cgtgccgctg cccgtcttct ttgacgaaaa cggcaaagcc gtcctggacg ccgctctggt
     2461 tcgcaaaatc gcggatatgg tggagaaaca cggttccaac atctggtttg aactctccga
     2521 tgaagacctg tgcgaacgcc tcggcctgcc gaagacctgg aaaaagggca aggatacgct
     2581 ggacgtctgg attgattccg gcagttccca tatggccgtc atggacaacc gccccgggct
     2641 ggacgcgccg gcggaccttt acctggaagc tacggaccag caccgaggct ggttccagag
     2701 ttccctgatg ctttccgtcg cctggcgcgg caaggctccg tacaaggccg tgatgaccca
     2761 cggcttcgtc gtggacaaag ataccgggaa aaaaacctcc aaatccgacg ccaagagcgg
     2821 caaacccatt gacgcggcct attactacga caaatacggt gcagacatcg tgcgcctgtg
     2881 ggccgcttcc gtggactggc aaaatgaagt ccccttcggg gaagacctct tcaagcaggt
     2941 gacggaaccc taccgccgcc tccgcaacac cctgcgcatc ctgctgggca acatcagcgg
     3001 attcgatttc gccgcgcagg cagtcgcccc ggaacacatg cccctcctgg accgctggat
     3061 tctggaacgc ctgaacgccg tcatcaggga aacgctgaag gcctatgaag cctatgactt
     3121 ccgcaaggcg ttcagcgcca tcaaccagtt ctgcaccagc gacctgtccg ccctgtacgt
     3181 ggacaccacc aaggaccgcc tgtactgcga ctcatcttcc tccgtgcgcc gccgcgccac
     3241 acaaacggcc atgaccatca tcttccaggc tctgtgccgt ctgctggctc ccatcctggc
     3301 cttcacggcg gatgaagcct gggaatacgc gggatatgaa ggcagcgtgc atgagcagga
     3361 cttccccgcc cccatgccgg aatacgatac ccaggaggcg acctccgtca tttcccgtct
     3421 ccaggaaatc aagtccgtca ttcaggtagc cattgaagaa caggtgaagg ccaaggtatt
     3481 ttccaaaaat aatgaggcct ccatcactct gagcgttccc gtgaatgaga gcgaagacgt
     3541 cgtggcgctt ctggaagacc gggcgtttgc cacggagttc ttcatcatcg cggaccttga
     3601 cgtgaaaacc ggtccggaac tggcagcctc cgacgcaaaa acggaacatg ccatgtgccc
     3661 acgctgccgc cggtatgaac cggcggcgga gggcagggac gtatgcgaac gctgcgcgga
     3721 agttctttcc tgaaccgcat ccactccatt tcccgacctt tatatggacc cccagacgga
     3781 aaaagaaccc gccccccata aaaagacgcc tcccggctgg ctctggtgga gcttgctggg
     3841 cgcagggctg tatgcgctgg accaggccag caagctgtgg attatccgcc gcttcccgct
     3901 gaactacctg aacgaaaagc tccataagta cgcctatctg ccggactttc cgggcttcca
     3961 gcacatcaat gcggacggca cgctggactt cgccacgaac ggacgcatgc cggaagcgga
     4021 actggcgcgg ctcccggacc tgaatccaaa cacggtggac gccctgaaca aactgaggga
     4081 actggaaccc atttccttcc tggacggaac catgaacatt acccgcgtgc acaacacggg
     4141 cgttgctttc gggctgggga acggcaccgt ctggtccagc tatctttttc tggcggttcc
     4201 cgtgctcgcc atcgtggtcc tggtcgtgct ttaccggaaa aacttcttcc acacggcatg
     4261 gctcaagctg gcctatgttc tgctgctggc gggcgtagcc ggcaacctga cggaccgcct
     4321 gattcaaggc ttcctgattc cgtatgagca gcagcacggc ttcttcacca agctgatgaa
     4381 cggttacgtg gtggacttca ttgacgtcac catccccctc ttcaactacc gctggcccgc
     4441 cttcaacgtg gcggactcct gcatcttcgt agcggcaatt attttcttca tcgccagtat
     4501 tttctccgcc aggaataaag aagaaaaacc atcctgaaac gcccatgacc atccggctcg
     4561 ccattctgtc aggactgctt ttgtgcggga gcctcgcctc cggcaaaaat gaattgtact
     4621 tcaaggaaac ggtggttccg gtaaaggtgg ctccggacca ggacagcatc acggcatcct
     4681 tccccttcac caacagttcc ggcaccccgg tcaccatcag taaaatccac gtttcctgcg
     4741 actgcaccac cgccggagcg aaggataaca agctgacgta tgcccctggg gaatccggcg
     4801 tcatcagcgc cgtgatgaaa accggcaatt tttccggtac cgtggacaag gacatgacgg
     4861 tgcatgccaa cggctccgcc tacaagctgg tgatacgcgc acaaatcccg gacatcatcc
     4921 ggatggagcc ccgcaagctg gaatgggcca ggggagaagc cgccgcgccc aaaaccatta
     4981 aaatcaccat ctccaaagaa ctgcccgtca acctgaccac ggtggatttg acgggagacg
     5041 cctttgacta cgagcctgtg accgtgaaaa aaggcaggga atataaaatc atcgtcactc
     5101 ccaaatccac ggccaggccg gccttcaaca ccatctgggt gcgcacggat tccgccgtgc
     5161 cgcgctacaa gcgccaaatg gggttcctgg ccatcaagga aaactgacat ccgcccttcc
     5221 ttccatgaga gacgcttttt ccaccgctct gaaactcctg ctggccgtat ttctgctggc
     5281 cgtaggcgtc gccgcgctgg acctgcgcgt catccagccg ttccgcacgc cgccctgcaa
     5341 cccggaaacg ctggaggaag ggcacgtgtg cctctcccag atattaaagg aatggccggg
     5401 gaaaatcctc tggatagacg ccagaaaaca ggacgacttt gaacgccata ccgtcacaca
     5461 ggctcccgtt taccccatcc gcccggcgga tgccaattac caggaactcc tggcgaatgc
     5521 catggaagcc ctgatgacgg cagaggacag gggattctgc atcgtcatct tttgcagcag
     5581 ggactgcacc tccagcgcgg ccgtggccaa tgaactgaaa aagccggaat acggcatccg
     5641 agcgcccatt ttcattctgg aagggggctg ggatgaattg cgcaaagaac cctcactcgt
     5701 accatagcca tgcttgacac agcaaccatt cgcccgcagt tccccattct ggaaaccagc
     5761 gtgcacggca agcctctcat ttacctggac aatgcggcca ccacgcaaaa gcccctggcc
     5821 gtactggacg ccatccgcca ttactacgat acggaaaacg ccaatgtgca ccgcggttcc
     5881 cactacttga gccagctcgc gacggaagcg catgaagaat cgcggaaaac ggtggcgcgg
     5941 ttcatcaacg cgccggaaac ggcggaagtc ctgttcacct ccggctgcac gatgggtatc
     6001 aacctggcgg cggataccat cgccgggtcc ggcatggtca aaccgggaga cgaagtcatt
     6061 gttaccgctt ccgaacacca ttccaacatc gttccctggc aaatgctgtg cgaacgcacg
     6121 ggcgccgtcc tgaaagcggt tcccctgacg ccgggccaga ccctggacat ggaagcttac
     6181 cggaacatgc tttccccccg cacccgcatc gtagccgtgg gacacgtttc caacacgctg
     6241 gggacagtca atcccgtgcg ggaaatgacc gcgctcgcca aagcgaacag gcag
//
LOCUS       DAOMSU010000115         6226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1051_length_6226_cov_2.764544, whole genome shotgun sequence.
ACCESSION   DAOMSU010000115 DAOMSU010000000
VERSION     DAOMSU010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1051_length_6226_cov_2.764544"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="PK077_06115"
     CDS             <1..650
                     /locus_tag="PK077_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23912.1"
                     /translation="PEQENVFPAISEQMGDYMTISADPSKQEDSEANRTDNSGASAYL
                     VILGDDFLHSIWGNKKTLSMSPVQEETVARNTEDGESGTMRAYTSRLEGWTGIAVESP
                     FSVARIKNISAQHPLTDKLVAKAKSLFPAALRGMISYVVMNGNVKLLLQESRTLTPAT
                     GNGGTGMIAPEPDSVMGIKILEVDSLLDDESLSSVRAAFAEDFFRARRNSLALKN"
     gene            683..1078
                     /locus_tag="PK077_06120"
     CDS             683..1078
                     /locus_tag="PK077_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23913.1"
                     /translation="MMKNMYRNDEALTIRLKMPGTGKTVTSAPIHIGQKGGIDSAVIS
                     LKHEELPALAAGKTMTLTVESSEDGDTWTELDSPKLVATGGESNGSGSGEVFMRVPLE
                     AGPWLRLKIAAETSAGDSTAQEAVLAVKV"
     gene            1092..1505
                     /locus_tag="PK077_06125"
     CDS             1092..1505
                     /locus_tag="PK077_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23914.1"
                     /translation="MALVRITPEAVARYCQDKEITSIARDKISDIIREVCNEVAAAVN
                     SCPRNARIAMDSSSVPAELVFTTCILVRDAVTSSVPGSSESLQGTARAAQYQDARAKL
                     RAVAACEVEFAPYDGHQPSDVIYGGPKHQDWSNPI"
     gene            1502..1972
                     /locus_tag="PK077_06130"
     CDS             1502..1972
                     /locus_tag="PK077_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23915.1"
                     /translation="MKKTLKKSPVIAFAEVLCQRAVEICSAANNGEDPEIIIKAWDGS
                     FEEEIKRVTGSLETVIVMERPEIVPDKLSRSGKSTARWHVTVESNPLLDGDGWDADDL
                     ADIIQEGFHKWRRNHARLMMTEVIVTSSKPALAKILKKSIVLTMETTLIIKHGN"
     gene            1962..2168
                     /locus_tag="PK077_06135"
     CDS             1962..2168
                     /locus_tag="PK077_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23916.1"
                     /translation="MATKPTTAAAQEAATAPAPRIVKCRVAVNKLELPHGIAARGKIV
                     HIPEDVYKVHADAGKVTFIDYVRS"
     gene            2174..2755
                     /locus_tag="PK077_06140"
     CDS             2174..2755
                     /locus_tag="PK077_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23917.1"
                     /translation="MSELYNKEMLVGTFLDLCPFGTTVTAGSGTDTVDEQFKPAKDSD
                     AWMMANEVIDYKITPTTEDDARTVFSRDTTSYVTRKNTKVTGNTIEINSTEVNPVCWQ
                     VIYQCDRLEAGKEVQPFSRNIYGQKVWARLTKYQEDKKEMMVLEVAALLKVEIPTENN
                     KLITPKLTLEVIPSSLNSLTPTEEIAFPASAGA"
     gene            2846..3253
                     /locus_tag="PK077_06145"
     CDS             2846..3253
                     /locus_tag="PK077_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23918.1"
                     /translation="MDTTVSPFSITFDGRPVVRAGEFLLDSLPEHAFPVQFGTSATPI
                     INSPFPRLDAFGNLSLSFTISTVRECASHMDAWGAFYEWLNEWKTAGKGEWAWTDACG
                     REQRFEAVIADAEPKVQGLRLIVSYNFTLGRPL"
     gene            3250..5433
                     /locus_tag="PK077_06150"
     CDS             3250..5433
                     /locus_tag="PK077_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23919.1"
                     /translation="MKTLDVSSSDFLDMAESPSYNRLSFGGASVSFRAPVSRFASCPF
                     EEGEIVKVVWRGKTLLIGPAIDLEHSLEGTSESWDIRICDYWWNLSNIQYFANGRANG
                     IFAEYRQGTGGSGQEKQATANIRDALSGVLDHAVSTALVPIKYDLRIDKDAEIIPFAY
                     ASETYASLLSQIQQWRPNMAAWFEYGADDSATLVIADHAALPDVVLDLSAVDVSALSL
                     KARPDLVPPAVGLTCNASVVSRVQRALAVYPSGASLSQPYVVTAEVDVPGGVKVSDTA
                     GQYSPAETGSLGYDAPRMIVRGDKFPTGTAQWAARVKRWAPALEDCAGLEVAASPKIT
                     SITPADAEHRGYSSAAVTHELTSGQINGKSARIKWGRVRVDLRVRATEPPDTVKQYFP
                     EYGGKSGTGDRWIGTLTFEVTTTNVGYASYRVDRAGTVESVSDDGGSPGDDETSGSYD
                     TSVLYKNFLKSYYEATRALPYDGSATVHDDFDQVCGGRLSITGGLKEWETMRSVILKT
                     GVSDVTVGAPEQISLQDSIDRSRQLAEALRRTAWADSSTSSGGGSSGGGSGSGGSSGA
                     DDEVPELPSVGPSVKLLQAQEPPAWGTSAVEVGFQCRLSYGSDGKVSDAYIRQGKAIY
                     AGNYIGGLLPEGDGSGGWVKSPVTSGEIWLKIQLDKDAKYLGSSLSAAGGVSDPVRLA
                     EENRETPYEYYFHLATIDGNKVVQHQAGTVYLLIHPGTFGPTGMS"
     gene            5430..6167
                     /locus_tag="PK077_06155"
     CDS             5430..6167
                     /locus_tag="PK077_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23920.1"
                     /translation="MIRIYTFTYAGDAQEAVACVRCARTALPEAVVTVVDDSAAPVPP
                     EARRALVAYGARYRRSSFPRCGNLRGPECVRGIIATLAKGAADGDTVVKIDSDTALLS
                     GGWVREMKHNGLALHAAGYRVPRNPSERSAYGNCYALSGRAARMAAEALECAAIPPLA
                     PEDLTICRAVMDVCGRERVRLDEPWTPRNRAGRWSWWNWDSRTANPEDYARSYDVVSV
                     GNPRPPHVPKSARREVMRALCDARLNP"
BASE COUNT         1338 a         1931 c         1868 g         1089 t
ORIGIN      
        1 ttccggaaca ggaaaatgtg ttcccggcta tttctgaaca gatgggggat tatatgacca
       61 tttccgcaga tccttccaag caggaagact cggaagccaa ccgcaccgac aactccggag
      121 cttccgctta cctggtcatt ttgggtgacg acttcctgca ctccatatgg gggaacaaga
      181 agacgctttc catgtctccg gtgcaggaag agaccgtggc caggaatacg gaagacgggg
      241 aatcaggaac aatgagggcc tatacttccc gtttggaagg ctggacgggc attgccgtgg
      301 aatctccgtt ttccgtggcc cgcatcaaga acatcagcgc ccagcatccc ttgacggaca
      361 aacttgtcgc caaggcgaag agcctgtttc ctgcggcctt gcgcggcatg atttcctatg
      421 tggttatgaa cggcaatgtg aaattgctgt tgcaggaatc cagaaccctt acgcctgcca
      481 ccggaaacgg cggaacgggc atgatcgccc ctgaacccga ttccgtgatg ggaatcaaga
      541 ttctggaagt ggattccctg cttgatgacg aatcactgtc cagtgtccgc gccgcatttg
      601 cggaagactt tttccgcgcc cgtcgcaact cccttgccct caaaaattaa ccttttatcc
      661 gcagaaagga gaaacacacc acatgatgaa gaatatgtac cgcaatgacg aagcgcttac
      721 gatccgtctg aagatgccgg gaaccggaaa gacggtaacg tctgccccga ttcatatcgg
      781 acagaaagga ggcatcgaca gcgctgtcat ttcattgaag cacgaagagc ttcccgcgct
      841 ggccgccggc aagacgatga ccctcaccgt cgaatcgtcc gaggacggtg atacctggac
      901 ggaactggat tccccgaagc tggttgcgac ggggggtgag agcaatggtt ccggctccgg
      961 agaagtgttc atgcgcgttc cgttggaggc cggcccctgg ctgcgcctga aaatcgcagc
     1021 tgaaacgtcc gcaggcgaca gcacggcaca ggaagccgtc cttgccgtca aggtataacc
     1081 ttattgaaac aatggccctc gtaaggatta ctccggaagc ggttgcccgc tattgccagg
     1141 acaaggaaat tacttccatt gcccgggaca aaatcagcga catcatccgc gaggtctgca
     1201 acgaggtggc ggctgcagtc aactcctgcc ccagaaatgc caggattgcg atggattcca
     1261 gttccgttcc cgcggagttg gtattcacca cctgcattct ggtgcgggat gccgtcacca
     1321 gctccgtgcc aggttcaagc gaatccctgc aggggacggc gcgggcggct caatatcagg
     1381 atgcccgcgc gaaactccgc gccgtggctg cctgtgaagt cgagtttgcc ccctacgatg
     1441 ggcaccagcc cagcgacgtc atttacggag ggccgaaaca ccaggattgg agcaatccga
     1501 tatgaagaaa accctgaaga agtcgcctgt cattgcattt gcggaagtcc tctgtcagcg
     1561 ggccgtggaa atttgctccg cggccaacaa cggggaagac ccggaaatca ttattaaggc
     1621 atgggacggt tcctttgagg aagaaatcaa gagggtgacc ggttccctgg aaaccgtcat
     1681 cgtcatggag cgtccggaaa ttgttccgga caagttgagc aggagcggca aaagcacggc
     1741 cagatggcac gtcaccgtgg agagcaaccc gcttctggac ggtgacggct gggacgccga
     1801 cgaccttgcc gacatcatcc aggagggctt tcacaagtgg cgccgcaacc atgcccggct
     1861 gatgatgacg gaggtaatcg ttaccagctc caagccggct ctcgccaaaa tcctgaaaaa
     1921 gtccatcgtc ctgacgatgg aaacaaccct gattatcaaa catggcaact aaacccacca
     1981 ccgccgcggc ccaggaggcc gctactgctc cggcgccccg catcgtcaaa tgccgggtgg
     2041 ccgtcaacaa gctggaactc cctcacggca tcgccgcgcg gggaaaaatc gtccacatcc
     2101 cggaagacgt gtacaaagtc cacgccgacg ccgggaaagt gacctttatt gactacgtaa
     2161 gaagctaaca accatgtcag aactctacaa caaggaaatg ctggtcggca cctttctcga
     2221 cctgtgcccg ttcggaacga cagtcacggc cggaagcggc acggacacgg tggacgagca
     2281 attcaagccg gcaaaggact ccgacgcctg gatgatggcc aacgaagtca tcgactacaa
     2341 aatcacgccg accacggaag acgacgcccg cacggtattt tcccgcgaca cgacctccta
     2401 tgtgacgcgg aagaacacca aagtgacggg caacaccatc gaaatcaact ccacggaggt
     2461 taatccggtc tgctggcagg tgatttacca gtgcgacagg ctggaagccg ggaaggaagt
     2521 gcagcccttt tcccggaaca tctacgggca aaaggtatgg gcgcgcctca ccaaatacca
     2581 ggaagacaaa aaagaaatga tggtcctgga agtcgcggcg ctgctcaagg tggaaatccc
     2641 cacggaaaac aacaagctga tcacgccgaa attgacgctt gaagtgatcc cgtcctccct
     2701 gaattccctg acgcccacgg aagaaatcgc cttcccggcc tccgccgggg catgacagcc
     2761 ggggccgccc ttctgtttgc atggggggcg gccagtcgcg ctccgcaagg tgtgcgtgga
     2821 ttgaaacccc caccattatt gaggcatgga cacgaccgta tctccctttt ccattacctt
     2881 tgacgggcgc cccgtcgtgc gcgccgggga attcctgctc gactccctgc cggaacacgc
     2941 tttcccggtg cagttcggca cgtccgccac gccgatcatc aacagcccgt tccccaggct
     3001 ggacgcattc ggcaacctgt ccctgtcctt caccatctcc accgtgcggg aatgcgcctc
     3061 ccacatggac gcgtggggcg ccttttacga atggctcaac gaatggaaaa cggcggggaa
     3121 gggggaatgg gcctggaccg acgcctgcgg ccgtgaacag cgctttgaag ccgtcatcgc
     3181 cgacgccgag ccgaaggttc agggcctgcg ccttattgtc tcctacaact tcaccctggg
     3241 ccgccccctg tgaaaaccct tgacgtatct tcctccgact tcctggacat ggccgaaagc
     3301 ccgtcctaca accggctctc cttcggggga gcctccgtct ccttccgcgc gccggtctcc
     3361 cggtttgcct cctgcccgtt tgaagaaggg gaaatagtga aagttgtctg gcgcgggaaa
     3421 accctgctca tcggcccggc catcgacctg gaacactccc ttgaaggaac ctccgagagc
     3481 tgggacatca ggatttgcga ttactggtgg aacctgagca acatccagta cttcgcgaat
     3541 ggccgcgcca acggcatctt tgccgaatac cgccagggca caggcggaag cggtcaggaa
     3601 aaacaggcga ccgcgaacat ccgggacgcc ctctccggag tcctggacca cgccgtcagc
     3661 acggccctgg tccctatcaa atacgacctc cggatcgaca aggatgccga aatcattccg
     3721 tttgcctacg cgtcggaaac gtatgcctcc ctgctttccc agatccagca atggcgcccc
     3781 aacatggccg cgtggtttga atacggcgcg gacgactccg ccacgctggt cattgccgac
     3841 catgccgccc tgccggatgt cgtgctcgac ctgtccgctg tggacgtaag cgccctgtcc
     3901 ctcaaggcgc gccccgatct ggtgcctccg gccgtggggc tgacctgcaa cgcttccgtg
     3961 gtctcccggg ttcagcgcgc gctggccgtc tatccctcag gcgcctccct gtcccagccc
     4021 tatgtggtga cggcggaagt ggacgttccg ggcggcgtca aggtctccga cactgccggg
     4081 caatacagcc ctgcggaaac gggctcgctg ggttacgacg ccccgcggat gattgtccgg
     4141 ggagacaaat tcccgaccgg cacggcccag tgggcggccc gcgtcaaacg ctgggctccg
     4201 gctctggagg attgcgccgg cctggaagtg gcggccagtc cgaaaatcac gtccatcacg
     4261 ccggctgacg cggaacaccg gggatacagc agcgcggccg tcacccacga actgacctcc
     4321 ggccagatca acggaaagag cgcgagaatc aaatggggca gggtccgggt ggatttgcgg
     4381 gtgcgggcga cggagccccc cgacacggtg aagcaatatt ttccggaata cggcggaaaa
     4441 tccggaaccg gggaccgctg gatcggaaca ttgacgtttg aagtgaccac gacgaatgtc
     4501 ggctacgcgt cctaccgggt ggacagggca gggacggtgg aaagcgtatc cgacgacggc
     4561 ggaagccccg gagacgacga aacatcgggc agctacgaca cctccgtgtt gtataaaaat
     4621 ttcctgaaat cctactacga agccacccgc gcgctgccct atgacggatc cgcgaccgtc
     4681 cacgacgact ttgaccaggt ctgcgggggg cgcctctcca tcacgggagg attgaaagaa
     4741 tgggaaacca tgcggtccgt catccttaaa acgggagttt ccgacgtgac ggtgggggcc
     4801 ccggaacaga tctccctgca ggactccatc gaccggagcc ggcagcttgc cgaggcgctg
     4861 cgccggacgg cctgggcgga ctcgtccacg tcttccgggg gaggttcttc gggcggagga
     4921 tccggcagcg gaggctcttc cggagcggac gatgaagtcc cggagcttcc cagcgtcggg
     4981 ccgtccgtaa aactgctgca ggcccaggag cctcccgcgt ggggaaccag cgccgtcgag
     5041 gtgggattcc aatgccgcct gtcttacggg agcgacggca aggtgtccga cgcctacatc
     5101 cgccagggga aggctatcta tgccggcaac tatatcgggg ggctgcttcc ggagggggac
     5161 ggttccgggg gctgggtgaa aagccccgtc acctccgggg aaatctggct caagatccag
     5221 ctggacaagg acgcgaaata cctcggatcc tctctgtccg ccgcgggcgg cgtctccgac
     5281 cccgtcaggc tcgcggagga aaaccgggaa accccttatg aatattattt ccatctggcc
     5341 accatcgacg gcaacaaggt ggtgcagcac caggcgggca cggtttatct cctaatccac
     5401 ccgggaacct tcggccccac cggaatgtca tgatcaggat atacaccttc acctatgccg
     5461 gagacgcgca ggaagccgtg gcctgcgtcc ggtgcgccag gacggctcta ccggaggcgg
     5521 tagttacggt ggtggacgac agcgcagccc cggtgccccc ggaggccagg agggctcttg
     5581 tagcgtatgg ggcgcggtat cgccggagct ctttcccccg ctgcggcaac ctgcgcggcc
     5641 cggagtgcgt ccggggaatc attgccacgc tggccaaggg ggcggcggat ggcgataccg
     5701 tcgtcaagat tgactccgac acggcgcttc tgtcgggcgg atgggtcagg gagatgaaac
     5761 acaacgggct tgcgctgcac gccgccggat accgggtccc ccggaacccg tccgaacggt
     5821 ccgcctacgg aaattgctac gccctgagcg gccgggcggc caggatggcc gcagaagctc
     5881 tggaatgcgc cgccatcccc ccgctcgccc cggaagacct aaccatctgc cgggccgtca
     5941 tggatgtctg cggccgggag cgtgtccggc ttgacgagcc gtggacgccc cggaaccggg
     6001 ccgggcggtg gtcctggtgg aactgggaca gccggacggc gaatccggag gactatgccc
     6061 gcagctatga cgtggtgagc gtcggcaatc cccggcctcc ccacgttccc aaaagcgccc
     6121 gtagggaagt catgcgcgcc ctgtgcgacg cccgtttgaa tccatgaatg ctccggcaac
     6181 cacggatatg tcccccttca actacccgct gaaacaacaa cagcca
//
LOCUS       DAOMSU010000116         6204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1054_length_6204_cov_2.691657, whole genome shotgun sequence.
ACCESSION   DAOMSU010000116 DAOMSU010000000
VERSION     DAOMSU010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6204
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1054_length_6204_cov_2.691657"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            704..2206
                     /locus_tag="PK077_06160"
     CDS             704..2206
                     /locus_tag="PK077_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HRN23921.1"
                     /translation="MVTFYNITLSTAFSLLMLSVAEAARPNVVLINADDLGWAEVGCY
                     GQQKIKTPNIDKLASEGQRWVYFYSGAPVCSPSRNVLMTGKHTGSCDVQDLKRVDAGE
                     NWRDLKGDWPIRTETYTLPEAMKKAGYATAVFGKWGIGDFGSTGAPDKHGVDRFYGYT
                     DQKACHTYYPPYLWNDGKKEVLNTSLTAATIGHGSQPKGEVLADTYRAEQHSSDLIAD
                     KMLEFVKEKAHGKQPFFLYYAPLEPHVAMQPLQEWIDRYPREWDKSPYRGNRGYLPHP
                     RPRAAYAGMISQMDHNVGRLLDTLKACGLDKNTIVIFTSDNGTTHDAGGVDHRFFNSV
                     ADLKGLKGQLYEGGIRVPGIIRWPGKIAPGKTITQPAFHADVMPTLCALTGADAGSPL
                     GMDLSPVLLGKKSALHDRKPLVWAGGGYGGQVAVRFDSKKVIRRNLFPGKKPDNWEVY
                     DIVKDPAEKNNIAAENRDLINRAIAILDREYQPAPGFQALRYKAPEQVAE"
     gene            2291..3256
                     /gene="bioB"
                     /locus_tag="PK077_06165"
     CDS             2291..3256
                     /gene="bioB"
                     /locus_tag="PK077_06165"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HRN23922.1"
                     /translation="MSLTSSLLSRVLGGGSCSKEELIALSREPLEELCQAANAIREHF
                     CGNVFDLCTIINGRSGKCSENCKYCAQSAHYSTAVEEYPLLNDEALLAGARYNDERGI
                     LRYSIVTSGKRLTDEDVDRLCASYRHIAEHCGISLCASHGLISKKHCEQLKAAGVSRY
                     HNNLETSRRNFPNVCTTHTYDDKLQTIKWALEAGLEVCSGGIMGLGETMEDRIDMYMD
                     IAALGIKSMPVNFLTPIPGTPYADMTPLGEEEQLRIVALVRFIMPDGFVRIAAGRNTM
                     KDHGRKIFMSGANAAISGDMLTTAGVTIREDLAMLAELGYEVRMK"
     gene            3285..>4527
                     /gene="bioA"
                     /locus_tag="PK077_06170"
     CDS             3285..>4527
                     /gene="bioA"
                     /locus_tag="PK077_06170"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HRN23923.1"
                     /translation="MNHSDWSKKDLEYIWHPCSQMKDYETLPPIVVDHGKGVNLYDVD
                     GKRYLDVVSSWWCNLLGHSHPKINRAIKEQLDSLEHVIFANFSHRPAITLCEELMKKI
                     PRGLCKFNFSDNGSASIESAMKMSFQYHYQTGNRQKVRFMSLSDAYHGETLGALSAGG
                     LDLYSELYKPLLLDIVRIPAPDCYRCPKGNKRGCCQAECIDAAQEAFARHGDECAALL
                     VEPLLQGSAGMRMYPPAYLAKLRSLCDAYNVHLIADEIATGFGRTGSMFACDQAGVSP
                     DIMCVSKGLTGGYMPMSITITTQKIYDAFYADYREGKAFMHSHTYSGNPLGCSAALAV
                     LKVLDEEQIIPRAQEKAPLFRRMIVEALGNHPHVGEIRSLGLVNAIELVENRETKKSF
                     PSEQRMGYRIYREALKRGLLLR"
     assembly_gap    4528..4537
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4538..4729
                     /locus_tag="PK077_06175"
     CDS             <4538..4729
                     /locus_tag="PK077_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23924.1"
                     /translation="GDALRELLHHLRPDVLVRKHRREAQLLRPLGDVLYFNPPLVISP
                     EEMEEAVSICASCIRKVLG"
     gene            5056..5712
                     /locus_tag="PK077_06180"
     CDS             5056..5712
                     /locus_tag="PK077_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HRN23925.1"
                     /translation="MNFEVWYQQELQIQYFRNEVDNKGEMLMGPVNLLKAKNRDELRQ
                     WLEQNHKTEKECWVVVKRGKPKGNNTFWYIDAVEEAMCFGWIDSTTKKLDNGITAQKL
                     APRRTGSLWSELNKERCRRMEKLGKMTEVGRSALPDMSSAGFIIDKEILKALQTDNEV
                     WENFLKFPPLYQRVRIDTIQIKKKQPQLFQSRLQKLIKNTKKGIMYGEWNDNGRLSDY
                     "
     gene            5780..>6204
                     /locus_tag="PK077_06185"
     CDS             5780..>6204
                     /locus_tag="PK077_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23926.1"
                     /translation="MQSNTAMIRLTPLRKRPALSALVLLLMGSGFCQAQEMLTDSARK
                     EASEWLKEVKAGYLNTSSSRIQKAVAALTAATSTENAAMKLYVDAMKDRFLNPTSMMS
                     RMLNRGGGGGFRMMRMPGSGGKGGNSSKPDSPSTAFSNWR"
BASE COUNT         1645 a         1707 c         1534 g         1308 t
ORIGIN      
        1 gaatcaaacc aacgcatcgg aacgcctttt tctagcggat ttacaatccg ttacggacag
       61 taaaagaaaa aaactctcgt tcccagagaa agtgccgctc tttatacggt tattgaaacg
      121 tgactccgct tcaacatgca acgaactcta taaaagaggg tttgccaaaa ttctacttca
      181 ggcccggata tcgtagcaat ttcccgtatt cccgtcacca gggtggaccc aggcagattt
      241 ctgttctaca taagagaccc tgatacgtaa agaaacgacg ttgcaagttc ttttctctta
      301 gagttacatc ttgactccgg attgtaaatc cgcccatctt ctcaagaaag cactatttca
      361 ttgaaaaatg cttcacccag aactattttc attctgaatt tccggttagc atcttaagaa
      421 aagtatcaaa aaatctgctc gggatgatat gaaatagcct tgccgccggt tgatgacctg
      481 tccgacaagc aaattgctgc cacaaatatt tacgattcat cattccatat aaaaatataa
      541 ataaaaagga accggacaaa caggaacggc gcggataaat aagtagtccg aaacaaaagg
      601 aaaccgaaag gctcaccatg tgagcatctc atccggcatc cggtaaggaa aacgaaacta
      661 ttttttgaaa caaggaagag tgtttctccg ttattattac cgtatggtca cattttacaa
      721 catcacactc tctaccgcct tttccctgct gatgctgtct gtggcggaag cggcacggcc
      781 caatgtggtt ctcatcaatg cggatgacct tggctgggcg gaagtgggct gctacggtca
      841 gcaaaaaatt aaaaccccga acattgacaa gctggcctcc gaaggacagc gatgggttta
      901 tttctattcc ggagctccgg tttgttcccc ttcccgcaat gtgctgatga cgggcaagca
      961 tacgggcagc tgcgacgtac aggatttgaa acgcgtggac gcgggcgaaa actggcgcga
     1021 cctcaaagga gactggccca tcagaacgga aacctacacc ctgccggaag ccatgaaaaa
     1081 agccggttac gccacagcgg tgttcggtaa atggggtatt ggggatttcg gttccaccgg
     1141 agcgccggac aaacacggcg tggacaggtt ctatggctac acggaccaga aagcctgcca
     1201 cacctactat cctccatacc tctggaatga cggaaagaag gaagttctca acacttccct
     1261 gacagccgcc accatcggac acggttccca gcccaaaggg gaagttctgg cggacaccta
     1321 ccgcgcggaa caacacagtt ccgatcttat tgcagataaa atgctggaat ttgtgaagga
     1381 aaaggcccat ggcaaacaac cgtttttcct gtattacgcc ccgctggaac cccatgtggc
     1441 catgcagcct cttcaggaat ggattgaccg ctatccccgc gaatgggaca aatcccccta
     1501 ccgcggcaac cggggatatc tgccccatcc ccgcccccgg gccgcctatg caggcatgat
     1561 ttcccagatg gaccacaacg taggacgcct gctggacacg ctgaaagcct gtggcctgga
     1621 caaaaatacc atcgtcattt ttaccagcga caacggcacc acgcatgatg caggaggggt
     1681 agaccaccgg ttcttcaact ccgtagccga tctcaaaggt ttgaaagggc agctttatga
     1741 aggcggtata cgtgtccccg gcattatccg ctggcctggg aaaatagccc cgggaaaaac
     1801 catcacccag ccggccttcc atgcggacgt gatgcctaca ctgtgcgctc tgacaggagc
     1861 ggatgcaggt tctccgctgg gaatggacct ctcccctgtc cttctgggca aaaaatccgc
     1921 tctgcatgac aggaagcccc tggtctgggc agggggaggc tacggcggcc aggtagccgt
     1981 gcgtttcgac tccaagaaag tcatccgccg caacctgttt cccggtaaaa aaccggacaa
     2041 ctgggaagtg tacgatatcg tgaaagaccc cgcagagaaa aataatatcg ccgcagaaaa
     2101 ccgtgacctt atcaacagag ccatcgccat tctggacagg gaatatcaac ccgcgcccgg
     2161 cttccaggcc ctgcgttaca aggccccgga acaggtagcc gaataagaga acgggaagga
     2221 agactccacg atccgcctcc ttcaccattc tgaccttgac gtgaacggcg ccggggttag
     2281 tctttcacgc atgagtttga catcatccct cctttccagg gtgctcggag gcggttcctg
     2341 ctccaaagaa gaattgatag ccctcagccg ggaaccgctg gaagaactgt gccaggcggc
     2401 caacgccatc cgggaacatt tttgcgggaa tgtcttcgac ctctgcacca tcatcaacgg
     2461 ccgcagcggc aaatgttcgg aaaactgcaa gtactgcgcc cagtccgccc attactccac
     2521 ggcagtggag gaatatcccc ttttaaatga tgaagccctc ctggcggggg ccaggtacaa
     2581 tgacgaacgc ggcattctgc gctactccat cgtcacctcc ggcaagaggc tgacggatga
     2641 agacgtggac cggctctgcg ccagttaccg gcacatcgcc gaacattgcg gcatctccct
     2701 ctgcgcctcc cacggcctta tttccaagaa acactgcgag cagttgaaag ccgcaggcgt
     2761 ttcacgctac cacaataatc tggaaacttc ccgccgcaat ttccctaatg tctgcaccac
     2821 ccatacgtac gacgacaaac tgcaaaccat caaatgggct ctggaagccg gtctggaagt
     2881 ttgcagcggc ggcattatgg ggcttggaga gactatggaa gaccggatag acatgtacat
     2941 ggacatcgcg gcgctgggaa tcaaatccat gcccgtcaac tttctgaccc ccatccccgg
     3001 aacgccgtac gcggacatga cccccctggg agaggaagaa caactgcgca tcgtggccct
     3061 ggtgcggttc atcatgccgg acggcttcgt ccgcatcgcc gccggaagga acaccatgaa
     3121 ggaccacggc aggaaaatct tcatgtccgg agcgaatgcc gccatctccg gagatatgct
     3181 caccaccgcc ggcgtcacca tccgggaaga cctggccatg ctggcggaac tgggatatga
     3241 agtccgcatg aaataaggtt cgttccattt tcgcgccctt tgccatgaat cactccgact
     3301 ggtcaaaaaa agatctggaa tacatctggc atccctgttc ccagatgaag gattacgaaa
     3361 cgctgcctcc tatcgtggtc gaccacggaa aaggcgtcaa cctgtatgat gtggacggga
     3421 aacgctatct ggacgtggtc agctcctggt ggtgcaacct gctgggccat tcccacccca
     3481 aaatcaaccg tgccatcaag gaacagcttg attccctgga gcacgtcatc ttcgccaact
     3541 tctcacacag accggctatc accctgtgtg aagaactaat gaagaaaatt ccgcgggggc
     3601 tctgcaaatt caatttctcg gataacggtt cggcatccat cgaatccgcg atgaaaatga
     3661 gttttcaata ccactaccag accggaaacc gccagaaagt acgtttcatg tccctgagcg
     3721 acgcctacca cggagaaacc ctgggagccc tctccgcagg aggcctggac ctttattcgg
     3781 aactctacaa gcctcttctg ctggacatcg tgcgcattcc cgctccggat tgctaccgct
     3841 gccccaaggg gaacaaacgc ggctgctgcc aggcggaatg cattgacgcg gcacaggagg
     3901 ccttcgcgcg ccatggggac gaatgcgccg ccctgctggt ggaacccctg ctccaaggct
     3961 ccgcaggcat gcggatgtat cctcccgctt atctggcgaa actgcgttcc ctgtgcgacg
     4021 cctacaacgt gcacctcatt gcggatgaaa tcgccaccgg ttttggccgc acggggagca
     4081 tgttcgcctg cgaccaggcc ggcgtttccc cagacatcat gtgcgtctcc aaagggctca
     4141 cgggcggcta tatgcccatg tccatcacca tcaccacgca gaaaatttac gatgcctttt
     4201 atgcggacta cagggaagga aaagcattca tgcacagcca tacctattcc ggcaatccgc
     4261 tggggtgctc cgccgctctc gccgtgctga aggtcctgga cgaggaacaa atcattcccc
     4321 gggcgcagga aaaggcccct cttttccgcc ggatgatcgt ggaggccctg gggaatcatc
     4381 cccacgtggg ggaaatacgc agcctgggac tggtgaacgc cattgaactg gtggagaaca
     4441 gggaaaccaa aaaaagcttt ccgtccgaac agaggatggg gtaccggatt tacagggaag
     4501 cccttaaacg gggactgctg ctccgccnnn nnnnnnnggc gatgccctcc gcgaacttct
     4561 gcatcatctg cgcccggatg tccttgtccg aaaacaccgc cgagaagcgc agctgctccg
     4621 ccccctgggc gacgtcctgt atttcaatcc ccccctcgtc atttctccag aggaaatgga
     4681 ggaagccgta tccatctgcg cttcctgcat ccgcaaagtg ctgggataaa tgttcatccg
     4741 gcacaacaag acgaactgtt ctttcttgaa gaagattgtg aaccaccaat atagtgcaat
     4801 tataatttga caaaagccgg gatgcggcag ctacaataca gccgccgcgc cccggttttc
     4861 ttatgaatgc agataccaaa atgttacgca atagaacggc attttcggga gtaaagtata
     4921 aaccttattg tcttatttcc ccgcccggaa agccgcataa atcaaagctt ttttatcccc
     4981 tatcgcgtca cattcgcccg ctttttcctc atgtgctcgg cgttatgtct gcgggtgatc
     5041 ccgttgtaag cactcttgaa ttttgaagta tggtatcaac aggagctgca aatacaatat
     5101 ttccgcaatg aagttgacaa taaaggagaa atgcttatgg gaccagtcaa tttgttgaaa
     5161 gctaaaaatc gtgatgagtt aaggcaatgg cttgaacaaa atcataagac tgaaaaagaa
     5221 tgttgggttg ttgtaaaacg tggtaaacca aaagggaata acacattttg gtatattgac
     5281 gcagtagaag aagctatgtg tttcggctgg attgatagta caacaaaaaa actcgataat
     5341 ggtatcactg cacaaaaact ggctccccgc agaacaggaa gcttatggtc tgaactaaat
     5401 aaggaacgct gccgcaggat ggaaaaatta ggaaaaatga cggaagtcgg acgttctgct
     5461 ttacccgata tgtcatctgc aggttttatc atagataaag aaatcctaaa agcattacaa
     5521 actgataacg aagtgtggga aaattttctt aaatttccac ctctttatca gagggtacgc
     5581 atagatacta tacagataaa aaagaaacaa ccacaacttt ttcaaagcag attacaaaag
     5641 ttaattaaaa atacaaaaaa aggaataatg tatggcgaat ggaatgataa tgggcgatta
     5701 tcagattatt agctccggca accacgtttt aaaacaaaag atgcaacttt tcttcgggac
     5761 aggggtttgt aaggatacct tgcaatctaa taccgccatg atacgcctca ccccgctccg
     5821 caaacgtccg gcgctttccg cgctggtgct tcttcttatg ggctccggct tctgccaggc
     5881 tcaggaaatg cttaccgatt ccgcaagaaa ggaagcttcc gaatggctga aggaagtcaa
     5941 agccggctac ctgaacacca gctcttcccg catccagaag gccgtggccg cccttaccgc
     6001 agccaccagc acagaaaacg ccgccatgaa actttacgtg gacgccatga aggaccggtt
     6061 tctgaacccc accagcatga tgtcccgcat gctgaaccgg ggaggcgggg gaggcttccg
     6121 gatgatgcgc atgccgggtt ccggagggaa gggaggcaac agcagcaaac ctgacagccc
     6181 cagcaccgcc ttctccaact ggcg
//
LOCUS       DAOMSU010000117         6109 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1084_length_6109_cov_2.324248, whole genome shotgun sequence.
ACCESSION   DAOMSU010000117 DAOMSU010000000
VERSION     DAOMSU010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1084_length_6109_cov_2.324248"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>570
                     /locus_tag="PK077_06190"
     CDS             <1..>570
                     /locus_tag="PK077_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRN23927.1"
                     /translation="KPEAKPSDLIIHVRLNDPDHDRQMKDLGILGVNILHAVFFKRDR
                     LEEFIGSLVDNLDPRLVEIDVVRFEGHGFSMVDNRLFALQLVQSGLTPATLFLANGQV
                     AQAADVLYKRPIVLMRGSFNPVCNLHLEVMAQVKKTFLSHINETQADRCMEICEISMN
                     NLLRGDDVDHLEFLDRADSLKALGKNVLIT"
     assembly_gap    571..580
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <581..1065
                     /locus_tag="PK077_06195"
     CDS             <581..1065
                     /locus_tag="PK077_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRN23928.1"
                     /translation="FARQGFPSGEIGQLLALMHEVDHNNLSGLLARYTREPIAIALSI
                     GLLNELFKEKWTEDIPGGILESFGRTFQNKTRLYVSPWLNRKSGEFVTARTFRAPEQY
                     VHLYQHFLANGLVVDVPFFNEFLLRHTPRDIQRMIAADEDIWKTLVPEEAHRSALHFR
                     "
     gene            1277..2548
                     /locus_tag="PK077_06200"
     CDS             1277..2548
                     /locus_tag="PK077_06200"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HRN23929.1"
                     /translation="MNTIIIGSQWGDEGKGKMIDFLTESADVVARGQGGNNAGHTVIA
                     NGKKYILHLVPSGILWPDKLCVIGNGVVLDPIGLVEEINELRGQGVTITKDNLLISDR
                     AHVVLPFHKEMDAAQESALGKKAIGTTKRGIGPTYADKARRIGVRMADMRDPAIFDEV
                     LRRRIRMANAEMERMGLPAMDEEKMVAEVSAAADVLRPHITNTIPVMHEAVASGKSIL
                     FEGAQGAYLDVDFGTYPFVTSSNTSSAGACTGTGVPPHKIDRIIGVCKAYTTRVGAGP
                     FVTEDEDISNHLHSMGREFGATTGRPRRCGWADGVLLRFSAMFNGFDEMAMTNLDGYD
                     KCPEIKICTGYDLDGEILAYPPATVDEWERCKPVYETVPGWMQDISSCRSWEEIPEQA
                     KKFVKRMSELIGCPVTTVGVGPDREQTIAVQ"
     gene            complement(2623..2808)
                     /locus_tag="PK077_06205"
     CDS             complement(2623..2808)
                     /locus_tag="PK077_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23930.1"
                     /translation="MDDIYLLFPDLEAAKLPFSCVLEEAFPRFMQRCRVENKFFPCRC
                     RGETVAEKALKRVFGLY"
     gene            complement(2966..3673)
                     /locus_tag="PK077_06210"
     CDS             complement(2966..3673)
                     /locus_tag="PK077_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN23931.1"
                     /translation="MLSCVVASSLMVQGATVLTLDSSELQAAGGSYTLTEGINSGYGN
                     FSVTMALDAQAFQNAILAGNVKANIFSTNNNMIGLGLNYVSSGTPTAGIYGSWQGENY
                     NRTITSVNGSELNLNTGLKELFTENSYESAAITYQITSTGTWTYLTLVDSEGTVTTYG
                     GQDSGLKASGFGALTSFTYGTDYVKYLVVDNTTLQAGASQEANLNAIAAAAVPEPATA
                     SLSLIGLAALMMRRRRA"
     gene            4258..5742
                     /locus_tag="PK077_06215"
     CDS             4258..5742
                     /locus_tag="PK077_06215"
                     /inference="COORDINATES: protein motif:HMM:NF016830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HRN23932.1"
                     /translation="MRHAILPSMCAVAVAASCSPALAQQAAPAAAASPAYLNTERGLR
                     VKTLEGRAISPSLMKGMRPEEQITKRTPGVPTRPSVLLQHEGGTSAPGLEWFVARPLK
                     MMPYLDSSYVFGNTCAEPNAWIREDMISTGAQKLKTALSRYGITYSAQFGFNYTGLTG
                     GGLNGKTDFPSYNGSLLTNITIFRNHSTGSGLYLASEFDFGNGFDFNEGSKGPKTTLG
                     SLQNPTSSFQGPDPHLSNLSLAWVGAGGKLLLMAGQLDTTNYMDHNAYANSHNNNLTN
                     SVFGNNPVLPLTDNSLGFHFAWQPTTSFYVMGATAANNMAQNHNPFRNLNSDNWTNVL
                     EFGYVAEDFCSMGPGVYRLQPFFTTSNGENGGGVAVNFQQQLGRSSHLGWFFRGGWAS
                     DDAATLTGVRCAATTGLVLLSPFRGKGASNDGYLGLGLLWQQGAKKNGPYVNRNEYGI
                     ELTYVAQVTPTMTLQPDIQLVKNPVNGRRGQTAVVFQIQNVWSW"
     gene            5828..>6109
                     /locus_tag="PK077_06220"
     CDS             5828..>6109
                     /locus_tag="PK077_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase"
                     /protein_id="HRN23933.1"
                     /translation="MKRIQQPSMQEAEQASLNHEILNTWRPGETLGEHMERRGVSRRE
                     FNKWCLKMISLMGVATVTGGISNAQEIADKMAALKRPVVIWLQLQECTGC"
BASE COUNT         1445 a         1788 c         1570 g         1296 t
ORIGIN      
        1 gaaaccggag gcaaaaccgt ctgacctgat tatccacgtc cgcctgaacg acccggacca
       61 cgaccgccag atgaaggacc tgggcatcct gggcgtcaac atcctgcacg ccgtcttttt
      121 caaaagggac cgtctggagg aattcattgg atccctggtg gacaatctgg atccccggct
      181 ggtggaaata gatgtcgtcc gttttgaagg ccacggtttt tccatggtgg acaaccgtct
      241 tttcgcgctc cagctcgtcc agtccggcct gacgccggcc accctgttcc tggcgaacgg
      301 ccaggtggcc caggcggcgg atgtgctgta caagcgcccc atcgtgttga tgaggggcag
      361 tttcaacccc gtctgcaatc tgcatctgga agtcatggcc caggtgaaaa aaaccttcct
      421 ctcccacatc aatgagacgc aggcggaccg ctgcatggaa atttgtgaaa tatccatgaa
      481 taacctgctc cgcggcgatg atgtggacca tctggaattc ctggacaggg cggacagcct
      541 caaggctctc ggcaaaaacg ttctgattac nnnnnnnnnn ggttcgcacg ccagggtttt
      601 ccgtccggtg aaatcggcca gttgctggcg ctgatgcacg aggtcgatca taacaacctg
      661 tccgggctgc tggctcgcta cacgagagaa cccattgcca tcgccctctc catcggcctg
      721 ctgaatgagc tgttcaagga aaaatggacg gaggatattc ccggaggtat tctggaatcc
      781 ttcggccgca ccttccagaa caaaacacgt ctgtacgtct ccccgtggct caaccgcaaa
      841 tccggggaat tcgtcacggc acgcactttc cgcgccccgg aacaatacgt gcacctgtac
      901 cagcacttcc tggcgaacgg cctggtggtg gacgtgccct ttttcaatga attcctgctg
      961 cgccacacgc cgcgggacat ccagcgcatg atcgctgcgg atgaagacat ctggaaaacg
     1021 ctggttccgg aggaagcgca ccgttccgcc ctccacttcc gctgagttcc ttttcagcag
     1081 gccgcccctt cctccccgcg gcacggccct cccaatgccg cggtctctcc gttcctgaaa
     1141 ccggataatt cggcacggcc cctcccgcct gccctgccgg gcttcccgcc actggtcagg
     1201 aaaacttaac aattccgtaa gattgtcttg ccataaggca ggaatctcat taaggtctcc
     1261 gagttccatt tttatcatga ataccattat tatcggctcc caatggggcg acgaaggcaa
     1321 gggcaaaatg atcgatttcc tgacggaatc cgccgacgtg gtggcacgcg gccagggcgg
     1381 caacaacgcc ggccatactg taattgccaa tggaaagaaa tatattctcc acctggttcc
     1441 ctccggcatc ctgtggccgg acaagctttg cgtcatcggt aatggtgtgg tgctggaccc
     1501 catcggcctg gtggaggaaa tcaatgaact ccgcggccaa ggcgtgacca ttacaaaaga
     1561 caaccttctc atctccgacc gcgcccacgt ggtccttccc ttccacaagg aaatggacgc
     1621 cgcccaggaa tccgccctcg gcaaaaaagc catcggcacc accaagcgcg gcatcggccc
     1681 cacttacgcg gacaaggcac gccgcatcgg cgtgcgcatg gcggatatga gggatcctgc
     1741 gatttttgac gaagtgctgc gccgccgcat caggatggcg aatgcggaaa tggaacgcat
     1801 gggccttccc gctatggatg aagaaaaaat ggtggcggaa gtaagcgccg ccgcggatgt
     1861 gctgcgcccc cacatcacca acaccattcc ggttatgcac gaagccgtgg cctccggaaa
     1921 aagcatcctc tttgaagggg cccagggagc ttacctggat gtggacttcg gaacctatcc
     1981 gttcgtcacc tcctccaaca cctcttccgc cggcgcctgc accggcacgg gtgttcctcc
     2041 gcacaaaatt gaccgcatca tcggcgtctg caaggcctac accacccgcg tgggcgcagg
     2101 ccctttcgtc acggaggatg aagacatctc caaccatctg cacagcatgg gccgcgaatt
     2161 cggcgccacc acgggccgcc cccgccgttg cggttgggct gacggcgtgc tgctgcgctt
     2221 ctccgccatg ttcaacggtt ttgatgaaat ggccatgacc aacctggacg gctacgacaa
     2281 gtgcccggaa attaaaatct gcacgggtta cgatctggac ggggaaatcc tggcttatcc
     2341 gcccgccaca gtggatgaat gggaacgctg caagcccgtg tatgaaacgg tgcccggctg
     2401 gatgcaggac atttcctcct gccgttcctg ggaagagatt ccggaacagg ccaagaaatt
     2461 cgtcaaacgc atgagcgagc tcatcggctg ccccgtcaca accgtgggcg taggccccga
     2521 ccgcgaacag acgattgccg tgcaataaac ctcggcagtt ctcgttatta tttccttcaa
     2581 agcagttccg catgcatggc ggaactgctt ttttattgtc agctaataaa ggccgaacac
     2641 acgtttcaac gctttctccg ccaccgtttc tccacggcag cggcatggga aaaacttatt
     2701 ttccaccctg catctttgca tgaagcgcgg aaatgcctcc tctaaaacac atgaaaacgg
     2761 caattttgcc gcttccaggt caggaaaaag caaataaata tcatccaaaa cagcccggcg
     2821 gaactggtga agctgccgtt ggaaagccca tgcctgggag ttcccggaat gatttgacgg
     2881 atacggatac ttccctcctg atgataataa aagaggaggc gggatctttc ccgcctcctg
     2941 taaaacagca attggactcc ccggctcagg cacgtctgcg gcgcatcatg agggcagcca
     3001 gaccaatcag gctgagggat gccgtggccg gttcgggaac ggcagccgcc gcaatggcat
     3061 taagattggc ttcctgggaa gcaccagcct gcaacgtggt attatccacg acaaggtatt
     3121 tgacgtaatc cgtgccatag gtaaatgaag tcagggctcc gaacccgctc gctttcaacc
     3181 ctgaatcctg accaccataa gtggtgacgg ttccttcgga atcaaccaga gtcaggtaag
     3241 tccaggttcc ggtgctggta atctgataag tgatggcggc tgattcatag ctgttttcgg
     3301 tgaacaattc cttcaatccc gtattcaaat tgagttcaga accgttgaca gaagtaatcg
     3361 tgcggttgta gttctctcct tgccaggaac cataaatgcc cgccgtggga gttcctgaag
     3421 aaacgtagtt gaggcccagg ccgatcatat tattattggt ggaaaaaata ttcgccttga
     3481 cgttacctgc cagaatggcg ttttggaaag cctgagcgtc cagggccatg gtcacggaga
     3541 aattgccgta accggagttt ataccctccg tcagggtata tgatccgcct gctgcctgca
     3601 attcactgga atccaaagta aggacggttg ccccctgaac catgagggaa gaagctacta
     3661 cacaagaaag caaaaataat gttttcttca tttgactgtt ctaggttgaa aagaaaagag
     3721 cgacgcgctc tcccagtgcg gtacatatag gggatttaga atccgctgcg cacttaaata
     3781 ttttctcctt ctcataacat atttacctgt attcggataa gatgaaataa aaaaagacca
     3841 gaaattatat atgatattaa aaaacaactg attaagatga attagacgtt gattttcaat
     3901 atttgttatt gttcccggat tctaaattcg caataaaaat tacagagaaa atctgatgct
     3961 tttttcccgt tttacactcc ttttggctcc ctcacccgac gtacattcag acagccccct
     4021 gcaaaaaaca ataatctccc taagctacca tgttccaact ggttccactt cttttgcgga
     4081 aggaatacgg cggtgggagc tacccctccg cttgccctat catgcctgtc ttaggaaaag
     4141 gccctgaagg acagcccgcc tttccttttt ccggaaagac ggtgaaaaac gaatattcgt
     4201 gtcagcattt aaacggagtt atgatacttc cgggaaaagc cttgtctgac tgtctgtatg
     4261 agacatgcca ttcttccctc catgtgcgct gttgccgtcg cagcctcctg cagtcccgcc
     4321 cttgcccaac aggcggctcc cgctgcggcg gcttcccctg cttacctgaa cacggaacgc
     4381 ggtctgcgcg tgaaaactct ggaaggcagg gcaataagcc ccagcctgat gaaaggaatg
     4441 cgcccggaag aacaaatcac caaaaggacg cccggcgttc ccaccaggcc cagcgtactg
     4501 cttcagcatg aaggcggaac ttccgctccc ggcctggaat ggtttgtggc caggcccctt
     4561 aaaatgatgc cgtacctgga cagttcctac gttttcggca atacctgtgc ggaaccgaac
     4621 gcctggatac gcgaagacat gatttccacc ggagcgcaaa agctcaagac cgctctctcc
     4681 agatacggca ttacttattc cgcccagttc ggattcaact acacggggct gacgggcggc
     4741 ggcctgaacg gaaaaacgga tttcccctcc tacaatggtt ccctgctgac caatatcacc
     4801 atcttccgca atcattccac gggcagcggc ctgtacctgg cctccgagtt cgacttcggc
     4861 aacggctttg acttcaatga aggcagtaaa ggccccaaaa ccacgctggg ctcactccag
     4921 aaccccacca gctccttcca gggcccggac ccccacctga gcaacctgag cctggcatgg
     4981 gtgggagcgg gaggaaagct tctgctgatg gcgggccagc tggacaccac caactacatg
     5041 gaccacaacg cttacgccaa ttcccataac aataacctca ccaacagcgt tttcggcaat
     5101 aatcccgtac tccccctgac ggataattcc ctgggattcc atttcgcgtg gcagccaacc
     5161 acctcctttt acgtgatggg agccactgcc gccaataaca tggctcaaaa ccacaatcct
     5221 ttccggaacc tgaattccga caattggacg aacgtgcttg aattcggcta cgtggcggaa
     5281 gatttttgca gcatgggccc cggcgtgtac cgcctccagc cctttttcac caccagcaac
     5341 ggtgaaaacg ggggcggcgt ggccgtaaac ttccagcagc agctgggccg cagcagccac
     5401 ctggggtggt tcttccgcgg cggctgggca agcgatgacg cagccacgct gaccggcgta
     5461 cgctgcgcgg ccaccacagg gctggtgctt ctttccccct tccggggaaa gggcgcatcc
     5521 aatgacggat acctgggtct cggtctcctc tggcagcagg gggctaaaaa gaacggcccg
     5581 tacgtgaaca ggaatgaata cggcattgaa ttgacgtacg tggcccaggt gacgcccacc
     5641 atgactctcc aacctgatat tcagcttgtc aagaatcccg tcaacggccg cagagggcaa
     5701 acggcggtgg ttttccagat ccagaacgtc tggtcctggt aaaagccctc ccagaaaact
     5761 ggaaaatatt gatcgcattt cccggaaatc ctgtctaaat ggaaatgttc ttatttggac
     5821 cacgcacatg aagcgcattc agcaaccttc catgcaggag gcggagcagg cctccctgaa
     5881 tcatgagatt ttaaatacat ggcgtcccgg ggaaactctg ggggaacaca tggagcgccg
     5941 cggcgtcagc cggcgcgaat tcaacaaatg gtgcctgaaa atgatctccc tgatgggcgt
     6001 cgccaccgta acgggcggca tttccaacgc ccaggaaatt gcggacaaaa tggctgcatt
     6061 gaaacgtccc gtagtcatct ggctccagct tcaggaatgc accggatgc
//
LOCUS       DAOMSU010000118         6080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1093_length_6080_cov_2.888963, whole genome shotgun sequence.
ACCESSION   DAOMSU010000118 DAOMSU010000000
VERSION     DAOMSU010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6080
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1093_length_6080_cov_2.888963"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PK077_06225"
     CDS             complement(<1..371)
                     /locus_tag="PK077_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23934.1"
                     /translation="MNRRIRIILLAISLLLVIGTLGWLTHVLLVQAEETGHSEQRIRV
                     EALAHEAKLEMDRLLTSFITFEQARGYFEYQPVYAYKRPYDLRMGAPAPGEATRGSNL
                     AAYLPDYVTAFIQISPAGRVTG"
     gene            complement(375..1079)
                     /locus_tag="PK077_06230"
     CDS             complement(375..1079)
                     /locus_tag="PK077_06230"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRN23935.1"
                     /translation="MSDSAYTILIAEDDDSIRHALTDVLTASGYEVLALEEGLAAIHA
                     VRERNFDLALLDVAMPGADGFQVLQVMSEERPGTPVIMLTARGEEEDRVQGLKLGADD
                     YIVKPFSIRELLARIEAVLRRSPERPRQLREISIPGATLDSANRLITFKDGRETSLTA
                     REFEFLTYMATHPNRVITRDELLRRVWDVDPRLTDTRSVEMTVMRLRDKLGAASASPL
                     ETLRSQGYRWNSSFVS"
     gene            complement(1180..1626)
                     /locus_tag="PK077_06235"
     CDS             complement(1180..1626)
                     /locus_tag="PK077_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23936.1"
                     /translation="MASTTLTPHGHRTGGGRTGQNTALRMSTSTKIIVGIVIVVVMAL
                     TSFWGVYAPSFSEAYILPVQMTAADGTPLGDFQCRPVPAENGKTLLVFRDADFLRQTT
                     GGMVHSKIILSYKGTPFFEGIVPGPMEAIEIPSGSLPPPFRELRHN"
     gene            complement(1608..2603)
                     /gene="hemB"
                     /locus_tag="PK077_06240"
     CDS             complement(1608..2603)
                     /gene="hemB"
                     /locus_tag="PK077_06240"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009961939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HRN23937.1"
                     /translation="MMNLPIRPRRNRKSANIRGLIRETSLSPEHLIYPVFVHEGTGNQ
                     PIPSLPGCTRWSVKGLVEEAKRLMDLGIRTLDLFPAIPDEKKTPDACEACNPDGLIPR
                     TIYALKSEVPGITVMTDVALDPYNSDGHDGLVEFRSDGTMEILNDDSVEVLCRQALCH
                     ADAGADIVSPSDMMDGRVAAIRATLDSEALDDVSIMAYTAKYASALYGPFRGALESAP
                     KEGDKKTYQMDPGNIREALREAQLDEAEGADILMVKPATLYLDVMAAMRKQVTLPIAA
                     YHVSGEYLMIKSAAASGWLDERETVLETLISIRRAGADMILTYYAPQAAEWLQQR"
     gene            complement(2658..4280)
                     /locus_tag="PK077_06245"
     CDS             complement(2658..4280)
                     /locus_tag="PK077_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23938.1"
                     /translation="MNILPLSLFCLCLCCGFSRGGQPPEGAPVRFRINMDQAAGNKAP
                     STGPVPDQSTPLNAAKLLLRALQEKTDWKHEPYKSLPATSPFVSQLRLKSILSRMEAV
                     QGYVTGAAPERPPFVDEDSIIAEGDLAMAYILLPDKANPYVYAATAVALVKKEGRWQA
                     SLTPGSFDNTFLPFDDRIRGEAEKLSSEAKKKIFGLAHQYSVAAVKAALEHIKRFRQE
                     NVKGTTDDELKNLLIRAVKGNNPALIAALLVSPYYTESAITQSARLIPVVKAMRLQDR
                     REENRYIQPTHLSFMTFPNTILVPLSPHPDDQMAPNSREEDQQEEANKNNAKKNGKDI
                     HSIGAMTILPPRMSMPDIDKPALIYRYALEKTKDPTDGLPVFKMNLTDAFASWNLKPD
                     ESTTARIMADFHRTYPPLSFPTPEEAVTAAGRALANRDSLAMMRMLAPSNFASVKEFE
                     DALSQLKTVMGRIPTPHTRVRGKDVPEVKAAYIPAARDGCAGIKATIGVRTAVGNEFS
                     VNNLHFIQTDGGWMLAGADAEQPLELEAPKAP"
     gene            4541..>6080
                     /gene="thrS"
                     /locus_tag="PK077_06250"
     CDS             4541..>6080
                     /gene="thrS"
                     /locus_tag="PK077_06250"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRN23939.1"
                     /translation="MSEHKERKTLEERQQMSDLERLRHSCAHVLATAICRLWPDAQLA
                     GGPAVDNGFYYDVELDHRISTEDFERIEEEMKKVVKENQVFQKEVISRADAMKMAESG
                     ELGALGPRSEPSRFKIDLLNDIPEDEEISLYRNGDFTDLCAGPHVGRTGNCKAFKIMS
                     VASAFYKGDKNRPMLQRIYGTCFPNRTQLDEHLERLEEARRRDHRKLGRELGLFCIDE
                     SVGQGLILWKPKGALIRRSLQDFITEELDKLGYSQVYTPNIGKLDLYRTSGHFPYYQE
                     SQYAPIPERDAMEKLSQEGASCAELFNGLATGTIEGYMLKPMNCPHHIKIYANDAHSY
                     RDLPVRLAEFGTVYRWEQSGELGGMTRVRGFTQDDAHIFCTPDQLAGEIRQCLGIVKT
                     IFGTLGMTDYRVRLSMRDPESDKYVGSPENWDKAEQALREAAEWLGADYSEEAGEAAF
                     YGPKIDFIVRDAIGREWQLGTVQVDYNLPERFDLHYTGADNKPHRPVMVHRAQRPGIG
                     VGGHI"
BASE COUNT         1190 a         1686 c         1960 g         1244 t
ORIGIN      
        1 ccggttacgc gcccggcagg ggaaatctga atgaaggctg taacgtagtc cggcaggtag
       61 gcggcgagat tggaaccacg ggttgcttct ccgggggccg gggcgcccat cctcaggtca
      121 taggggcgtt tgtaggcgta aacgggctgg tattcaaaat agccgcgggc ctgttcgaat
      181 gtgatgaagg aggtcagaag gcggtccatt tccagcttgg cttcatgggc cagggcttcc
      241 accctgatac gctgttcgga atggcctgtt tcttccgcct gcaccagcag aacatgggtg
      301 agccatccga gcgtgccgat gaccagcagc aggctgattg ccagcagaat gatgcgtatg
      361 cgccggttca cggatcagga aacgaaggat gaattccagc ggtagccctg gcttctaagc
      421 gtctccaggg gggaggcgga ggcggccccg agcttgtccc ggagacgcat gacggtcatt
      481 tccacggagc gcgtatccgt caggcgcggg tccacgtccc atacgcgccg gagcagttcg
      541 tccctcgtga taacgcggtt ggggtgggtg gccatatagg tgaggaattc gaattcccgc
      601 gcggtcaggg aggtttccct cccgtcttta aaggtgatca ggcggttggc gctgtccagg
      661 gtggcgccgg ggatactgat ttcccggagc tggcgcggcc gttccggaga cctgcgcagc
      721 acggcctcga tgcgggcgag cagttcccgg atgctgaacg gcttgacaat atagtcgtcc
      781 gcccccagct tgagtccctg cacgcggtct tcctcctctc cacgggccgt gagcatgatg
      841 acaggcgttc cggggcgttc ctcagacatg acctgcagca cctggaatcc gtccgcgccc
      901 ggcatggcca catccagcag ggcgaggtcg aaattgcgtt cccgcacggc atggatggcg
      961 gccaggcctt cttccagagc cagcacttcg tagccggatg cggtcagaac gtccgtcagg
     1021 gcatgcctga tgctgtcgtc gtcctccgca atcagaatgg tataggctga atcactcatc
     1081 gtttgattct ataaaaccac aatgtttcac ctgttgtata gagaaacctg cgccctttca
     1141 gtaacaatgc cataacaggc cggcttcccg ggggcatcct cagttatgcc ggagttcccg
     1201 gaagggggga ggaaggctgc cggagggaat ttcaatggcc tccatgggac cggggacaat
     1261 tccttcaaag aacggcgtac ctttgtagga aaggatgatt ttggaatgca ccatgccgcc
     1321 cgtagtctgg cgcaggaaat ccgcatcccg gaagaccagg agcgtctttc cgttttccgc
     1381 gggtacagga cggcactgga aatcgccaag cggtgttccg tcagccgccg tcatctggac
     1441 gggcagtata taggcttccg aaaaagaagg agcgtaaaca ccccagaagg aagtgagcgc
     1501 cataacgacg acgatcacga tgccgacaat gattttcgtg gaggtggaca tgcggagggc
     1561 ggtgttctga cccgtacgtc cacctccggt acggtggccg tgcggcgtca gcgttgttga
     1621 agccattcgg cggcctgggg ggcgtaatac gtaaggatca tatcggcccc ggcgcgccgg
     1681 atggatatta gagtctccag aactgtttcc cgttcatcca gccagccaga agctgcggcg
     1741 gacttgatca tcaggtactc cccgctgaca tggtaggcgg ctatgggaag ggtcacctgc
     1801 ttccgcatgg cggccataac gtccagatac aatgtggcgg gtttcaccat caggatgtcc
     1861 gctccctcag cttcatccag ctgcgcttcc ctaagggctt cccggatgtt gccgggatcc
     1921 atctggtagg tttttttatc cccttccttg ggcgcgctct ccagagctcc gcggaacggc
     1981 ccgtacagag cgctggcgta cttggcggta taggccatga tggagacatc gtccagggct
     2041 tcggaatcca gggtggcgcg gatggcggcc acgcggccgt ccatcatgtc gctgggggag
     2101 acgatatccg ccccggcatc cgcgtggcac aatgcctggc ggcagagaac ctccacggaa
     2161 tcgtcgttaa ggatttccat cgttccgtcg gagcggaatt ccaccaggcc gtcatggccg
     2221 tcggaattgt agggatccag ggctacgtcc gtcatgacgg tgatgcccgg cacttcgctt
     2281 ttgagcgcgt aaatggtgcg cgggatgagg ccgtcagggt tgcaggcttc gcaggcgtcc
     2341 ggcgtttttt tctcatccgg aatggctgga aaaagatcca gcgtccggat gcccagatcc
     2401 atcaggcgct tagcttcctc caccagtccc ttgacgctcc agcgcgtgca gcccggcagg
     2461 gaggggatgg gctggttccc ggtcccttca tggacgaaga cgggatagat gaggtgttcc
     2521 ggggaaaggg aggtttcccg gataagaccc cggatgttgg cggattttct gttgcggcga
     2581 ggccgtatcg gtagattcat catggtgtgg taatgaaaag gtttttagga aggcatggcg
     2641 ttccgggacg gatatgatca gggagccttg ggggcctcca gttccagcgg ctgttccgca
     2701 tcggccccgg cgagcatcca gccgccgtct gtttggatga agtgcaggtt gttgacggag
     2761 aattcatttc caactgctgt acgcacgccg atggtggcct tgattccggc gcagccgtct
     2821 ctggcggcgg gaatatacgc cgctttgact tcgggaacgt cttttcccct taccctggtg
     2881 tgcggggtgg gaatccttcc catgactgtt ttcaattggg agagcgcgtc ttcaaattcc
     2941 ttcacggagg cgaagttgga cggggccagc atgcgcatca tggccaggct gtcccggttc
     3001 gccagggcgc ggccggcggc ggttacggcc tcttccggcg tgggaaagga cagcggagga
     3061 tacgtccgat ggaaatccgc catgatgcgg gcggtggtgg attcgtccgg tttcagattc
     3121 caggaagcga aagcgtccgt caggttcatc ttgaagacgg gaaggccgtc tgtgggatcc
     3181 ttggtttttt ccagcgcata gcggtaaatg agagcgggtt tgtctatgtc cggcatgctc
     3241 atgcgcggcg gcaggatggt catggctccg atgctgtgga tgtccttgcc gttcttcttc
     3301 gcgttgtttt tattggcttc ctcctgctgg tcttcttcac ggctgttcgg ggccatctgg
     3361 tcgtccggat gggggctcag gggaaccagg atggtattcg gaaaggtcat gaagctgagg
     3421 tgggtaggct ggatatagcg gttttcctcc cttctgtcct ggaggcgcat ggccttgacc
     3481 acgggaatga ggcgtgcgct ctgggtaatg gcggattccg tataatacgg ggaaacgagc
     3541 agggcggcga tgagggccgg gttattcccc ttcacggccc ggatgagcag gttcttgagt
     3601 tcgtcgtccg ttgttccctt gacgttttcc tggcggaaac gcttgatatg ttccagggcg
     3661 gctttaacgg cggctacgga gtactggtgg gcgaggccga aaattttctt cttggcctcc
     3721 gaggacagtt tttctgcttc tccccggatg cggtcgtcaa agggaaggaa tgtgttgtca
     3781 aagcttccgg gagtcaggga cgcctgccac cggccttctt ttttcaccag ggccacggcg
     3841 gtggccgcat aaacgtacgg gttggccttg tccggcagca ggatgtaggc cattgccagg
     3901 tcgccttcgg caatgatgga gtcttcatcc acaaagggcg gacgttccgg ggccgctccg
     3961 gtgacatacc cctggaccgc ctccatgcgg gaaaggatgc ttttcagcct gagttggctt
     4021 acaaaggggg aagtggcggg caatgacttg tacggctcgt gtttccagtc cgttttttcc
     4081 tgaagggcgc ggagcagcag tttggccgcg ttcaacggcg tgctctggtc cggaacgggt
     4141 cccgtggacg gagccttgtt tcccgcagcc tgatccatat tgatccggaa acggacagga
     4201 gcaccttccg gcggctggcc gccccgggaa aacccgcagc agaggcagag gcagaaaagg
     4261 gagaggggga ggatgttcat gaccgctggc cgccactatg gcataaacgg ctgcgggtgt
     4321 aaatcattcg ttgggaatgt tgacgcggcc gttgccgcag ggcggacaac cctggactcc
     4381 ggggaaaagc gaacaaaaag gggggaaagg atgcgttccg gaagaagaat ccgaaagccg
     4441 gaggaaaggg ctttcccgcg gcgttcatgc cttgaatttt cgggaagaag gtctttttgc
     4501 atttgccaac agcgtttaag agaggtttac tgatgcgcgt atgtccgaac acaaggaaag
     4561 aaagactctt gaagaacgcc aacagatgtc tgatttggaa cggctgcgcc actcctgcgc
     4621 gcacgttctg gctacagcca tttgccgcct ctggccggat gcccagctgg ccggcgggcc
     4681 tgccgtggat aacggttttt attacgacgt ggagctggac caccgcatca gcacagagga
     4741 ttttgaacgc attgaggagg agatgaaaaa agtggtgaag gaaaaccagg ttttccagaa
     4801 ggaagtcatt tcccgcgcgg atgccatgaa gatggcggaa tccggggagc tgggcgccct
     4861 gggcccccgc agcgagcctt cccgcttcaa gattgacctg ctgaacgaca tcccggaaga
     4921 cgaggagatt tccctctacc gcaacgggga ttttacggac ttgtgcgccg gtccccacgt
     4981 gggccgcacg ggcaactgca aggcattcaa aatcatgagc gtggcgagcg ccttctacaa
     5041 gggggacaaa aaccgcccca tgctccagcg catttacggc acctgtttcc cgaaccgcac
     5101 ccagcttgac gaacacctgg aacggctgga agaggcgcgg cgccgcgacc accgcaaact
     5161 gggccgcgaa ctgggcctgt tctgcattga cgaatccgtg gggcaggggc tcatcctctg
     5221 gaagcccaag ggggctctca tccgccgttc cctgcaggac ttcatcacgg aagagctgga
     5281 caagctgggc tactcccagg tgtacacgcc caacatcggc aagctggacc tgtaccgcac
     5341 gtccggacac ttcccgtatt accaggaaag ccagtacgcg cccatcccgg aacgggacgc
     5401 catggaaaaa ctcagccagg agggggcttc ctgcgcggaa ctgttcaacg gcctggccac
     5461 cggcaccatt gagggctata tgctcaagcc gatgaattgc ccccaccaca tcaagattta
     5521 cgcgaatgac gcccactcct accgggacct tcccgtgcgg ctggcggaat tcggcacggt
     5581 gtaccgttgg gaacagagcg gagagctggg cggcatgacg cgcgtgcgcg gtttcacgca
     5641 ggatgacgcc catatcttct gcacgccgga ccagcttgcc ggggaaatcc gccagtgcct
     5701 gggcatcgtg aaaaccattt tcggtacgct gggcatgacg gactaccgcg tgcgcctttc
     5761 catgcgcgac ccggaaagcg acaaatacgt gggttctccg gaaaactggg acaaagcgga
     5821 acaggctctg cgggaagctg cggaatggct gggggcggac tacagcgagg aagcagggga
     5881 agccgccttc tacggcccca agatcgactt catcgtgcgc gatgccatcg gccgcgaatg
     5941 gcagctggga accgtgcagg tggactacaa cctgccggaa cgctttgacc tccattacac
     6001 cggagcggac aacaagccgc accggcccgt gatggtgcac cgcgcccagc gccccggcat
     6061 cggtgtcggc ggccacatct
//
LOCUS       DAOMSU010000119         6060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1099_length_6060_cov_2.961865, whole genome shotgun sequence.
ACCESSION   DAOMSU010000119 DAOMSU010000000
VERSION     DAOMSU010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1099_length_6060_cov_2.961865"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..>1119
                     /locus_tag="PK077_06255"
     CDS             138..>1119
                     /locus_tag="PK077_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HRN23940.1"
                     /translation="MSANQDNNRELRENRRRRSRFILPAPRHLAAVAAGALLAAVLVH
                     YLGFIVLSWFDLGIHVHKAPTVQEDRKPLPEKIVLKADDRPAPDVAEAVEKPDIEQLE
                     EIPPELPDLEDMLPEQVVIAPGETSFSADPVSPSPPESLSPKMPQVDMNAVAKGLPEP
                     SPPEMLKSSASPVTIKTAEPDMVNPDEWYNNKLKGAGGQDDSHLPDGSKSLSQLMAQS
                     SLGKDSGYSRLGADLLFEYNKAVMKNSARLSMLQLAGLMMKNPDTIFIVEGHTDSFGS
                     EEYNAVLSLMRANAVRQWLKDNGIALTRPNGECRLYIRACGASRPVVSIRG"
     assembly_gap    1120..1129
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1130..1541
                     /locus_tag="PK077_06260"
     CDS             <1130..1541
                     /locus_tag="PK077_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23941.1"
                     /translation="QAANRRVEIHMRKPGEEIPAGSLPLTYAVDMSTPVARQVRAGVG
                     ARAGIPSSAAERKAPPAGRSSSASPERRENPALRQEVIPEAEPIPETIPSAEPVEERI
                     PSAEPVEDDIPLAEPVVCAGSRAVVKGGAGLRWN"
     gene            1532..2407
                     /locus_tag="PK077_06265"
     CDS             1532..2407
                     /locus_tag="PK077_06265"
                     /inference="COORDINATES: protein motif:HMM:NF019887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HRN23942.1"
                     /translation="MELMRVLPVRALPKPERLRYLFSFDFDDTLFTLGGPTEERSIFF
                     RTMRMLRSQYGVLWGVNTGRDPVYLREGLADMFRDDAEAFAPDFTVTMERNVHLADAE
                     GRLMPGAAWNDDCAVAHDSLFTRYGGMLESLIGQLESRFSGLGLRRQDNDAFSLVVDD
                     ACGLDEVSCVIQDTVGPYEEIVTQRAGPYLRFSHRDYNKGTSLSFVASRFGIPSSHVA
                     IFGDGHNDLDAMRHLPEAFRCCPSNAAQEVKDMVARGHGYISPEPRTRGVLDGLAHGV
                     FPYFGMKAEVLKEDI"
     gene            2550..4493
                     /locus_tag="PK077_06270"
     CDS             2550..4493
                     /locus_tag="PK077_06270"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HRN23943.1"
                     /translation="MNIPSFSRSVSRGSAVGWFLVLLLVCGAGAGYYLYQDNLAKRKA
                     AQELTAERKLKEKKAREAAEKQRIKREREIREKKEKERLAAQKAYEEAQEEKARQAAE
                     AARKLQEQAEREEREKRRREELERREREEEARRQEEDIPVKEEPEPEGRFPQPVKNRM
                     PELSVYSISCRDDIQTEKDKPLETWSWDKAEKMEGMEEFPTGSSPWKKGKDAGRMQAL
                     LEKCREWKDAKLASLKACPAAKDFPGVPENGAQTVRRTVEIDSNIGGWHSTGLYAPPG
                     AEISCSLSGAPKDGSISVRIGCHTDSLHKLDEWKRVPEITMQVPAGRGRVKMVNPMGG
                     LVYVNVGQRPRRGKVFKVQISGAVPSPLFVMGKTTPEQWAEQLENTKAPWGEIRMPRL
                     IVTMPVEQLKQCPDVQKTAEFLQKNMALQDWIMGWDTKPDRLHHPMRFVVDRQISAGA
                     GHSGYPAMATKDWTNSIATGSIIHSGSWGLWHELGHNHQSPPFTMEGQTEVSVNIFSM
                     VCEVMGTGKDFESCWGGGMGPYGMSAEMKKYFSGTQTYNEAPNKVQLFFWVELMYYLG
                     FDAFRQVALQFHDKPYDNGELSDEKKWEWVMNAFSKVTGKNMGPFFKIWRTPVSERAA
                     GRMKDLPAWLPSKDYPACYTAEE"
     gene            4578..5828
                     /locus_tag="PK077_06275"
     CDS             4578..5828
                     /locus_tag="PK077_06275"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN23944.1"
                     /translation="MKWLNDWLGFFKPLQVRNLQIFWSGQASALIGMWLQVTAMGILV
                     YDISGGSATAVGVLAALNALPFFLGGMLLAGLGDRFDRRKLLIAVQCVQWLVAVALFL
                     LTVLDMLQLWHLYAAGLVMGVNQTVGFPTQQAFVGDLIPRRQLQEAVGMYSLVFNTCR
                     AIGPALAGYIIAEWGAGTAFGGNVAASLPLVGCLVALKGRVADTSAPRKQRGAGRKAS
                     GLKAVLATRSLLFIMISALIQNICGQSLYQIVPALMHGNPRNTGLILGAVGAGAMVSI
                     LFVLPFARKSDRVGAKLSSGTLWMGCALCVAGAIPVVEVQALCFFFAGLATSSLFVTS
                     SSAVQLLSPPERKSAILGLFSIVTIGVQPLAAMGWGAVVDAWGVQMTIVVAGGVEALF
                     SIWMLGVPFWRHFKFSSDDCPETT"
BASE COUNT         1273 a         1538 c         1901 g         1338 t
ORIGIN      
        1 ggcattgcgg aatggattca tgagaatacg cgcatgccca tgcccagggt gttgcctcat
       61 gagtcttaca gtgcgcccgt tacgggtacc gctccttccg gttcatgaat tgttcctttc
      121 cttttgctga tgatcccgtg agtgcgaatc aggacaacaa ccgagaactc cgcgaaaacc
      181 gccgcaggcg cagccgtttt attctgcccg cgcctcgcca tctggcagct gtggcggcag
      241 gggctctcct tgccgccgtg ctggtgcatt acctggggtt tatcgttctg agctggtttg
      301 atctgggcat tcatgtgcac aaagccccca ctgtccagga ggacagaaag cctctgcctg
      361 aaaaaatagt tctgaaggcg gacgaccgtc ctgctccgga tgtagcggaa gccgttgaaa
      421 aaccggatat tgagcaactt gaagagattc ccccggaatt gccggattta gaggatatgc
      481 ttccggagca ggtagtgatt gctccggggg aaaccagctt ttccgcagat ccggtaagtc
      541 cttccccccc ggaatccctt tccccgaaaa tgccgcaggt ggacatgaat gccgtcgcga
      601 aggggttgcc cgaaccctct ccgcctgaga tgctgaaatc ttccgccagt ccggttacaa
      661 tcaagacggc tgaaccggat atggtgaatc cggatgaatg gtataacaat aagctcaaag
      721 gggcgggcgg tcaggatgat tcccatttgc cggatgggag caaatctctt tcccagctca
      781 tggcccagtc ctccttgggc aaggacagcg ggtacagcag gctgggagcg gatcttcttt
      841 ttgaatacaa taaggcggtg atgaaaaatt ccgcccggct gagcatgctt cagcttgcgg
      901 ggctgatgat gaaaaatccg gatacgattt tcattgtgga aggtcatacg gacagctttg
      961 gttccgagga atataatgcc gtgctttccc tgatgcgtgc taatgccgtc cgccagtggc
     1021 tgaaggataa cggcatagct ctgacgcgcc cgaatgggga atgcagattg tacatacgcg
     1081 cttgcggcgc ttcccgtccc gtcgtttcca tcaggggggn nnnnnnnnnc caggctgcca
     1141 accgcagggt ggaaatccac atgcgcaagc cgggagagga aatacccgcc gggagcctgc
     1201 cccttacgta tgcggtggat atgagcaccc ctgtcgcccg ccaggtgcgt gccggagtgg
     1261 gcgcccgcgc cggaattccc tcttccgcgg cagaaaggaa agcgcccccc gcagggcgtt
     1321 catcttctgc ctccccggaa cgccgggaaa atcctgcgct gcgtcaggaa gtaattcctg
     1381 aggcggaacc cattccggaa acgattcctt cggcagagcc tgtggaggaa cgcatccctt
     1441 cggcagaacc ggtagaggat gacatcccgc tggctgaacc cgtcgtgtgt gcggggagcc
     1501 gcgccgtagt gaagggaggt gccggattga gatggaattg atgcgtgtcc ttccggtccg
     1561 ggcgctgccg aaacccgaac ggctccggta tcttttctcc tttgattttg acgatactct
     1621 gttcactctt ggaggcccta cggaagaaag gagcattttt ttcagaacca tgcgcatgct
     1681 ccggtcgcag tatggtgttc tctggggcgt taatacgggg cgcgaccctg tttacctgag
     1741 ggaaggtctg gcggatatgt tccgggatga tgcggaagcg tttgccccgg attttacggt
     1801 gacgatggaa cggaatgttc atctggcgga tgcggagggc aggctgatgc ccggcgccgc
     1861 gtggaacgat gactgcgctg ttgcccatga cagcttgttc acccgttacg gcggcatgct
     1921 ggaatcattg attggccagc tggaaagccg gttttccggg ctgggactcc gcaggcagga
     1981 caatgacgct ttttccctgg tggtggatga tgcctgcggc ctggatgaag tttcctgcgt
     2041 cattcaggat acggttggtc cgtatgagga aattgtcacg caaagggcag gtccctacct
     2101 gcgtttcagc caccgtgatt acaataaggg aacatccctt tcctttgttg cctcccggtt
     2161 cggcattccg tcttcccatg tcgccatttt cggcgacggg cacaatgatc tggacgccat
     2221 gcggcacttg ccggaggcat tccggtgctg cccttccaat gcggcgcagg aagtgaaaga
     2281 catggttgcc cgcggccatg gatacattag cccggaaccg cgcacccggg gcgttctgga
     2341 cggcctggcg catggagtgt tcccttattt cgggatgaag gcggaggttt tgaaggagga
     2401 tatttgaaaa cgggagggcc tgatgctgac gggagatcgg ctttcaacat gtttctttca
     2461 tcaaggcgct cccggaaggg gaagaatcgt attgacaagg aatgcctgca atgcagaaat
     2521 gggaaagatt ctttcccaat cccgattaca tgaacattcc ctctttttcc cgtagcgttt
     2581 ccagaggttc cgccgtgggc tggtttttag tgttgctgct ggtttgcggt gccggagccg
     2641 gatattatct ttaccaggat aacctggcaa aaagaaaagc ggcccaggaa ttgaccgccg
     2701 aacgtaaatt gaaggaaaag aaggccaggg aagctgcgga gaaacagcga ataaaacggg
     2761 aacgggaaat cagggagaag aaggaaaagg aacggctggc ggcccagaag gcttatgagg
     2821 aagcgcagga agagaaggcg cggcaggcgg cggaagcggc ccgtaagctt caggagcagg
     2881 ccgaacgcga agaacgggaa aaaaggagaa gggaggagct ggaacggcgc gaacgtgagg
     2941 aagaggcccg caggcaggag gaagatattc ctgtgaaaga agagcctgaa ccggaagggc
     3001 gttttcccca acccgtgaaa aaccggatgc cggagctttc cgtttattct atttcttgca
     3061 gggatgatat ccagacggaa aaagataagc cgctggaaac atggtcctgg gacaaggcgg
     3121 aaaaaatgga gggtatggag gaattcccca cgggttcctc tccgtggaaa aaagggaaag
     3181 acgccggacg catgcaggcg cttctggaaa aatgccggga atggaaggat gccaaactcg
     3241 cctcgctgaa ggcgtgcccg gcggcgaagg attttcccgg cgttccggag aatggggccc
     3301 agacggtcag gcggacggtg gagatagatt ccaatattgg aggctggcat agtacgggac
     3361 tgtatgcgcc gcccggggcg gaaatttcgt gttccctgtc cggcgctccc aaagacggtt
     3421 cgatcagcgt ccgcatagga tgccatacgg acagccttca taagctggat gaatggaaaa
     3481 gagtgccgga aataaccatg caagttccgg ctggccgtgg gcgcgtgaaa atggtgaatc
     3541 cgatgggcgg ccttgtttat gtgaatgtag gccagcgtcc cagacgggga aaggtcttca
     3601 aggttcagat ttccggagcc gtgccttcac ctctgttcgt aatggggaag accactccgg
     3661 aacaatgggc cgaacaattg gaaaatacca aggccccgtg gggggaaatc cgcatgcccc
     3721 ggcttattgt cacgatgccc gtggaacagc tgaaacagtg tccggatgtt cagaagacgg
     3781 cggaatttct gcaaaaaaac atggctcttc aggactggat tatgggatgg gataccaagc
     3841 cggaccgcct gcatcatccg atgcgctttg tcgtggacag gcagatatcc gccggggccg
     3901 ggcattccgg ttatcccgcc atggccacga aggactggac gaattccatt gccaccggtt
     3961 ccatcatcca ttccggaagc tggggtttgt ggcatgaact ggggcataac caccaatccc
     4021 ctccctttac gatggaaggc cagacggagg tatccgtcaa catattctcc atggtgtgtg
     4081 aagtgatggg gactggaaaa gactttgaat cctgctgggg cggcggcatg gggccgtacg
     4141 gcatgagcgc ggaaatgaaa aaatattttt caggcaccca gacttacaat gaggctccca
     4201 acaaggtgca gctcttcttc tgggtggagc tgatgtacta tctggggttt gacgccttcc
     4261 gccaggtggc tcttcaattc catgacaagc cttatgacaa cggcgaactg agtgatgaaa
     4321 agaaatggga atgggtcatg aacgcttttt ccaaggtcac gggaaagaac atggggcctt
     4381 tctttaagat ttggcgtacg ccggtttccg aacgcgctgc gggcagaatg aaagaccttc
     4441 ccgcctggct tccttccaag gattatccgg cctgttatac cgcagaggaa taagaaggtt
     4501 ccggaacagg gggggaggag gcgttgccgc actcgcttct ggacggttcc gccggctggg
     4561 gctaggatgg cggagctgtg aaatggctga atgactggct gggctttttc aagccccttc
     4621 aggtgcggaa cctgcaaata ttctggtcag ggcaggcttc ggccctgatt ggaatgtggt
     4681 tgcaggtgac ggccatgggt attcttgtgt acgatatttc cggaggctcc gccacggcag
     4741 tgggggtgtt ggctgccttg aacgctcttc ctttctttct gggcggcatg ctgcttgccg
     4801 ggctggggga ccgttttgac cggaggaaac tcctcatagc cgtgcagtgc gtccagtggc
     4861 tggtggctgt agccctgttt ctgctgactg tgctggatat gctccagctg tggcacttat
     4921 acgccgccgg gctggtgatg ggcgtcaatc agacggtggg ttttcccaca cagcaggctt
     4981 ttgtgggaga cctgataccg cgccggcaat tacaggaggc cgtgggcatg tattctctgg
     5041 tatttaatac gtgccgggct atagggccgg cgcttgccgg ctacattatt gcggaatggg
     5101 gcgccggaac cgcctttggc ggcaatgtgg cggcaagcct tccgctggtt ggctgcctgg
     5161 ttgccttaaa gggacgcgtt gcggatacct ccgctcccag aaaacagcgc ggcgcaggaa
     5221 ggaaagcatc cggcctcaag gcggtgcttg ccacgcgcag cctgcttttc atcatgatca
     5281 gcgctctgat tcagaatatc tgcgggcaat ctctctatca gattgttccg gcattgatgc
     5341 acggaaatcc caggaatacc ggcctcattc tgggcgcggt cggagccgga gccatggtga
     5401 gcatcctctt tgtcctgccg tttgcacgca aaagcgacag ggtgggagcc aagctttcct
     5461 caggcaccct gtggatggga tgcgcactgt gcgtggcagg cgccatcccg gtggtggaag
     5521 tgcaggccct ctgtttcttt tttgccggtt tggccacttc ttccctcttt gttacttctt
     5581 catctgccgt gcagcttctg tcgcccccgg aacgcaagtc ggccattctg gggctgttca
     5641 gcattgtcac catcggtgtg cagccgctgg cggccatggg gtggggagct gtggtggatg
     5701 cctggggtgt ccagatgacg attgtcgtgg cggggggggt ggaagccttg ttttccattt
     5761 ggatgctggg cgttccattt tggcgtcatt tcaaattttc ttcggatgat tgtccggaga
     5821 cgacatagcg ccttcccttc catttgcggc aggggaagac tgtcggtcct catgctgcgg
     5881 ggaaaagggg tgcatgtagg gtttcccccc cgcggataat tccgggatgc agccggccag
     5941 ggaagaggaa gcaggttttg gaaacgtcag gagcaggaac cggctgttag ccgtccgttt
     6001 ctgcctcgtg ttcggaatag ctgatgccca gcatgcagat atcgtcggcc tgattggtgc
//
LOCUS       DAOMSU010000120         5973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1124_length_5973_cov_2.100203, whole genome shotgun sequence.
ACCESSION   DAOMSU010000120 DAOMSU010000000
VERSION     DAOMSU010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1124_length_5973_cov_2.100203"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            386..1048
                     /locus_tag="PK077_06280"
     CDS             386..1048
                     /locus_tag="PK077_06280"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRN23945.1"
                     /translation="MKQITSLRGLAIILIILFHLNPEHFPYGYFGVEVFLVISGYLLM
                     KSLQKQQMQIRLGEFARKKVLRLFTPVAVIVPLTLLAGMFFLDYTDLATASSTGGYTL
                     IGAANRFLAVIQSDYFAQDSSGNPFLHMWYLAVTAQIYLLFAGGCVLYRYLPKRAAAS
                     LFWIAGIGAFLWAYSYPIHNLLQEIGLPVWEQVRPTSHYMTLPRLWEVCAGILLAIYP
                     PL"
     gene            1102..2442
                     /locus_tag="PK077_06285"
     CDS             1102..2442
                     /locus_tag="PK077_06285"
                     /inference="COORDINATES: protein motif:HMM:NF039218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH hydrolase domain-containing protein"
                     /protein_id="HRN23946.1"
                     /translation="MLCILVPALSRGKDVSLCSAAVVLGTCLVIRYAESGRLAHCFNN
                     KLLLWIGGISFSLYLVHMPLIAFYHSWFLHAPGWPGMLAIGAASAVLGGLLYLAVEKK
                     RMKMKWFIPLYLFALLLCVAGRQTEGFRNYINKEINSISSPQLHGASEIKSPEAARGF
                     DKEAIKFNNNIFAFVSRADFKGSAEPGLLQLGSPAEAPSIVLLGDSHASAAFCGLDAA
                     CRELDISGIYVSSVISPFWNRELPALPFDASYYCDEKKIKALMAWLNVHPEISHVVIA
                     QHWSARYDSKNVMDWKLKPLPSGEPAYSDSLRAFISELRAMGKQVILLAPTPLIKQND
                     VAKYIRNLIRRGKTGKGQESLTCTRQKYREDHAAVLPILDRLEQEGLCTVLRILPYIP
                     EDRPFNAYEHGEILYKDSDHMSSKGSIKLFQHLKPQLKEALQKESTASSGLQKP"
     gene            2708..3586
                     /locus_tag="PK077_06290"
     CDS             2708..3586
                     /locus_tag="PK077_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23947.1"
                     /translation="MAAFSGMATADIQLPLDVLNANNGGKTFSAQLGAGYATKYISRG
                     LAFQNSASDHVIPLEAVGAYQLNNQYSLIGGLKYQWLTDNGLEHNDSGICDEGSAILG
                     VSRKFGKSTIAALSYQFVHGGIPGSFNIHNTGNNSGFPAFSHSRPEEHSFVLDIHHDF
                     GKGLENFFWDCRVQYTFRWMTGWWFTNTLGYKYDVNKSTSVIVAGTWNATAGYFDSNS
                     LNANGTQGISLTVSAPVKATKDLTVTPFVGTVWLGNGGLAANHRGASARFPYREPSKV
                     YRNFTFVAGVGVAYTF"
     gene            3666..4814
                     /locus_tag="PK077_06295"
     CDS             3666..4814
                     /locus_tag="PK077_06295"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRN23948.1"
                     /translation="MPLSAVFHTSSIQKQVAALSDAFAIAGEFLHCDVINSGHINMTF
                     RATYRKPDDTTRRYIFQRVNDAVFPCPKDVMHNVEKVTNHIRWKMLRVLKTPFRQTLN
                     LYSARGGRKYLEIPGSGFWRCYNCIENTHTFDVADHPRQAYEAARAFGAFQQLLCDMN
                     PEDIHETIPFFHHTRRRFDHLEKAAAADSHGRLNTCRKELDFIRRRERYVDVLLDLQE
                     RGELPVRIVHNDTKINNVMLDRETDKAVCVIDLDTVMPGSVLYDFGDMVRTMTSPAAE
                     DEENLDKTFLRMPMFEAVVKGYLESARDFITPQEVSKLAFSGLLITLETGIRFLTDYL
                     EGDVYFKTKKERHNLHRARTQLRLVESMEEQMPEMEECVRKCFQTVNG"
     gene            4885..5304
                     /gene="fucU"
                     /locus_tag="PK077_06300"
     CDS             4885..5304
                     /gene="fucU"
                     /locus_tag="PK077_06300"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HRN23949.1"
                     /translation="MLKNISPLISPSLLKILAEMGHGDEIVFSDAHFPGHTFNPTVLR
                     ADGLGADALLAAVIPLFELDAYATPVIMMAPVPGDSLDPAVEAKYRKALNYDGEIEQM
                     ERYAFYERSKKAYAVVITGETAKYGNIILKKGVTPLS"
     gene            5330..>5973
                     /locus_tag="PK077_06305"
     CDS             5330..>5973
                     /locus_tag="PK077_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRN23950.1"
                     /translation="MQYDFDEVIERRGTGALKYEALLPRWGRDDLIPLWVADMDFRTP
                     PFIMEAIRRRCEHEILGYTVKPRPFFEAIRDWVDRRHGWKVNASEIGFAPGIVPGISS
                     AIQCFTEPGDKIMIQPPVYHPFAMIIRENGREIVNNPLILENGRYRMDFNHMKEAVRG
                     CRMFILCNPHNPGGRVWSPEELRRVAEICTANGTLVVSDEIHADLTLPGRRHTV"
BASE COUNT         1424 a         1736 c         1437 g         1376 t
ORIGIN      
        1 ctagaactat cgctgacgaa aaaatttatt cctttttaaa aaaacagaaa taaatatcgc
       61 atcataattt ttcggcatta tttgctttag tcttgtttcg ttgatctttc cgatttttga
      121 tcggagcaat ttgctaaata tcaatatcta aaacattttg aaaatcttgc gccaagacta
      181 ttcttgcata attccgcatt tttttatcaa tcttttgatt agaaatcagg ccgcccatct
      241 gccggatgaa gtactaacgt tccatcctgc ttccggctcg gcatgagagt tattttatag
      301 ttatgccgtc agtggaaaaa acaatggaca gcattcagca tcccttcaca aatatatttc
      361 aaatttaaag tacatcttaa ttaacatgaa gcaaatcact tcactcagag gcctcgcgat
      421 tattctgatc atccttttcc acctcaatcc ggaacatttc ccctacggct acttcggcgt
      481 tgaagtgttt ctggtgattt ccggctacct gctcatgaaa tccctccaga aacagcagat
      541 gcaaatccgt ctcggggaat ttgcccgtaa aaaagtctta agacttttta ccccggttgc
      601 cgtcatcgtc ccgctcactc ttctggctgg catgttcttt ctggactata cggatctggc
      661 gacagccagc tccacaggcg gatatacgct gataggcgcc gcaaacaggt tcctggccgt
      721 catccagtcc gactatttcg cgcaggattc ctccggcaat ccgttccttc acatgtggta
      781 cctggcggtg acggcacaga tttaccttct gttcgcgggg ggatgcgtcc tgtaccgtta
      841 ccttcccaaa cgggcggccg cctctctttt ctggatcgcc ggcatcgggg cctttctctg
      901 ggcctattcc tatcccattc acaacctgtt gcaggaaatc ggcctgcccg tctgggagca
      961 ggtccgcccc acctcccatt acatgacgct gccccgtttg tgggaggttt gcgcagggat
     1021 tttgttagca atataccccc ccctttaagt tcaacacgtt gcgaaaacag caaaaaaaac
     1081 gctctcctct tcatccttgg aatgttatgc atcctcgtac ctgccctttc ccgcggaaag
     1141 gatgtctccc tctgctcggc ggccgtagtt ttggggacat gcctggtaat ccggtatgcg
     1201 gaatccggca ggctggccca ctgcttcaac aataaacttc tgctgtggat tggaggaatc
     1261 tcgttctccc tttacctggt gcatatgccc ctcatcgcgt tctatcattc gtggtttctc
     1321 catgcccccg gatggcccgg aatgcttgcc atcggagcgg cgtccgcagt cctgggaggt
     1381 ctcctgtacc ttgcagtaga gaaaaagcgc atgaagatga aatggtttat ccccctgtac
     1441 cttttcgcgt tgcttctatg cgttgcagga aggcagacgg aaggattccg gaactacatc
     1501 aacaaggaaa tcaactccat ttccagcccc cagctgcatg gcgcatcgga aatcaaatct
     1561 cctgaagccg cccggggatt tgataaggaa gccatcaaat tcaataacaa catctttgcc
     1621 ttcgtcagca gagccgattt caagggaagt gcggaacccg gcctgctgca gctgggttcc
     1681 cctgccgaag ctccatccat agtcctgctg ggggacagcc acgcctctgc ggccttttgc
     1741 ggattggacg cagcatgcag ggaattggat atctccggga tttacgtttc ttccgtcatt
     1801 tccccattct ggaacaggga attgcccgcg ctgccgtttg atgcaagtta ctattgcgac
     1861 gagaaaaaaa taaaggccct catggcatgg ctgaacgtcc atccggaaat tagccatgtg
     1921 gtgatagccc agcactggag cgcccgttac gacagtaaaa atgtaatgga ctggaagctg
     1981 aagccgcttc cctccggaga acctgcctat tcagactctc tccgtgcgtt tatctccgaa
     2041 ctgcgggcga tgggcaagca agtcatcctg ctggccccca ctccgctcat caagcaaaat
     2101 gatgtggcca agtacatcag gaacctcata cgcagaggga aaacgggaaa aggccaggaa
     2161 agccttacct gcacgcggca aaaatacagg gaagatcacg ccgccgtact tcccatcctt
     2221 gaccgtcttg aacaggaagg gctctgcacc gtcctgcgca tacttcccta tataccggaa
     2281 gacaggccgt tcaacgccta tgaacacggg gaaattctgt acaaggatag cgaccacatg
     2341 tccagcaaag gctccatcaa actcttccag cacctgaaac cccagttaaa ggaagcgcta
     2401 caaaaagaat ccacggcatc atcaggttta caaaagccat agccgtgaac acgtctgccg
     2461 cagctccggc ggggaaacat ttaaataaaa ccggcttttg tggaagctat agagttctct
     2521 ttgggaaaaa atgcccttgc atgatgaata atgcgtcaaa tgtttaacac tttttgtcat
     2581 acattgaaca tattttcata tttcttttct ggtattgaag atacacttgc taaaaataaa
     2641 aatattgtta ttatattttt cccctctaat tatgcaacat aaaaccatta ttgctacatg
     2701 tgcggccatg gccgcttttt ccggcatggc tactgccgat atccagcttc ccctggatgt
     2761 tttaaacgcc aataacggcg gcaagacatt ctccgcccag ctgggagcag gctacgctac
     2821 caaatacatc agccgcggtc tggctttcca gaattccgca agtgatcatg tcattcccct
     2881 ggaagccgtc ggcgcctatc aactgaacaa tcaatattcc ctgataggcg gattaaaata
     2941 tcagtggctg acggataatg gtctggaaca caatgattcc ggcatttgcg acgaaggctc
     3001 ggccatctta ggcgtcagcc gcaaattcgg aaaatccact atcgcggctc tgagttacca
     3061 gtttgtccac ggcggtatcc ccggaagttt caacatccac aatacaggca acaactccgg
     3121 cttccctgct ttcagccaca gcaggccgga agaacacagc ttcgttctgg acattcacca
     3181 tgacttcggc aaagggctgg aaaacttctt ctgggactgc cgcgtacaat atacgttccg
     3241 ctggatgact ggctggtggt tcaccaatac tctgggatac aagtatgacg tcaacaaatc
     3301 cacctccgtc atcgtggcgg gcacatggaa cgccactgcc ggctattttg acagcaattc
     3361 cctgaacgcc aatggaaccc agggcatttc cctgacggtt tccgcccctg tcaaggccac
     3421 gaaagacctg actgtgactc cgttcgtggg tacggtctgg ctcggcaacg gcggcctggc
     3481 ggccaaccac cgcggcgctt ccgcccgttt cccttacagg gagccctcca aggtttaccg
     3541 caatttcacc ttcgtcgccg gggtaggagt tgcctacacc ttctaacaat ctggctgtca
     3601 ttcactcaac caacgcaagc tccggcatgt gccatcaatc atgccggagc ttttcttaac
     3661 tccccatgcc cctgtccgcc gttttccaca cttcctccat tcagaagcaa gtggccgccc
     3721 tgagcgacgc attcgccatc gcaggagaat tcctccattg cgacgtcatt aacagcggcc
     3781 atatcaacat gaccttccgg gccacctaca ggaagccgga cgacaccacc cgccgctaca
     3841 tcttccagcg cgtgaacgat gccgtgttcc catgccccaa ggatgtcatg cacaacgtgg
     3901 aaaaggtgac caaccacatc cgctggaaaa tgctccgggt gctgaaaacg cccttccgcc
     3961 agacgctgaa cctgtactcc gcgcggggcg gccgcaaata tctggaaatt cccggttccg
     4021 gcttctggcg ctgctacaac tgcatagaaa acacccacac gttcgacgtg gcggaccatc
     4081 cccgccaggc ttacgaagca gcccgcgctt tcggcgcttt ccaacagctc ctgtgtgaca
     4141 tgaatccgga ggacatccat gaaaccattc cgttcttcca ccatactcgc aggcgctttg
     4201 accatttgga aaaagccgcg gcagcagact cccacggacg gctgaatacc tgccgcaagg
     4261 agctggactt catccgccgc cgtgaacgtt atgtggacgt gctgctggat ctccaggaac
     4321 ggggggaact ccccgtcaga atcgtccaca acgacacgaa aatcaacaac gtgatgctgg
     4381 acagggagac ggacaaggct gtctgcgtca ttgacctgga caccgtcatg cccgggagcg
     4441 tcctgtacga cttcggagac atggtacgca ccatgacctc ccctgcggcg gaagatgaag
     4501 aaaatctgga taaaaccttc ctgcgcatgc ccatgttcga ggccgtcgtc aagggatacc
     4561 tggagtctgc cagggatttc atcacgccgc aggaagtctc caaacttgct ttttccggtc
     4621 tgcttatcac gctggaaacg ggaatccgct tcctgacgga ctacctggaa ggggacgttt
     4681 atttcaaaac gaaaaaagaa cggcacaacc tgcaccgggc ccgcacccag ctcaggctgg
     4741 tggaaagcat ggaagagcaa atgcctgaaa tggaagaatg cgtccggaaa tgcttccaga
     4801 ctgttaacgg ctgagagggc atgaccggca ttaccccaat tttatgatag taccggaacg
     4861 cgggctggtg ctatactgcg gggcatgtta aaaaatattt ctcccctcat cagcccctcc
     4921 ctgctgaaaa ttcttgcgga aatggggcat ggcgacgaaa tcgtcttttc agacgcccac
     4981 ttccccggcc ataccttcaa ccctacggtg ctgagggcgg acggcctggg ggcggatgcc
     5041 ctgctggcag ccgtcattcc cctcttcgaa ctggatgcct acgcaacgcc cgtcatcatg
     5101 atggccccgg ttccggggga ctctctggac cctgcggtgg aagccaaata ccgcaaggcg
     5161 ctgaactatg acggggagat tgagcaaatg gagcgctacg ctttttatga acggtccaaa
     5221 aaggcctatg ctgtcgtcat taccggggaa acggccaaat acggcaacat catcctgaaa
     5281 aaaggcgtta cccctctttc ctgaccattg gccgccagac agaccttcca tgcagtacga
     5341 ttttgacgaa gtaattgagc gccgcggcac cggagccctg aaatatgaag cgctgcttcc
     5401 ccgctggggc cgggacgacc tgattcccct gtgggtggca gacatggact tcaggacgcc
     5461 ccccttcatc atggaagcta tccgccgccg ctgcgagcac gaaatcctgg gatataccgt
     5521 caagccccgc cctttctttg aagccatcag ggactgggtg gaccgacgcc acgggtggaa
     5581 ggtgaacgcc tcggaaatag gcttcgctcc gggcattgtt cccggcattt ccagcgccat
     5641 ccaatgcttt acggaaccgg gggacaaaat catgatccag cctccggtgt accacccctt
     5701 tgccatgatc atccgggaaa acggccggga aatcgtcaac aatcccctca ttctggaaaa
     5761 cggccgctac cgcatggact tcaaccacat gaaggaagcc gtgcgcggct gccgcatgtt
     5821 catcctgtgc aatccccaca accccggagg ccgcgtatgg agtccggagg aattgcgccg
     5881 cgtggctgaa atctgtacgg caaacggcac gctggtcgtt tctgatgaaa tccatgcgga
     5941 tctgaccctt cccggccgca ggcacaccgt att
//
LOCUS       DAOMSU010000121         5944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1135_length_5944_cov_2.676006, whole genome shotgun sequence.
ACCESSION   DAOMSU010000121 DAOMSU010000000
VERSION     DAOMSU010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1135_length_5944_cov_2.676006"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            29..1432
                     /locus_tag="PK077_06310"
     CDS             29..1432
                     /locus_tag="PK077_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HRN23951.1"
                     /translation="MSEEQHGSEENGGIRINKFLASCGIDSRRVADRLIGEGRVEVNG
                     KVIDTPGMRVTEKDFVKVDGRHMTPMEEVVVLLNKPRGYVCSREAQGAIGTVYDLLPP
                     RLRHLNYVGRLDADSEGLLIMTNKGELTQVLSHPTGGIEKEYWVTVDQNFDNSVLMQF
                     LRGVRIPEGNAKAKYVCRASARRACIVLEQGLKRQVRQMFQCLGLRVRKLVRVRIGSL
                     WGGDLEPGGWKFLDDADVALSLKNPPRQRKYLGASQLVAGGGAKGEQGGRGVDSDEDY
                     VFNPEDFEAGDSYEPTPEMKTRFVETEDEREVKEDGYFRGDSGFRSRDRRGDFHRRGD
                     GFRGRRGDGALRREAGFKEERRKGGFQRREGGFGRDRREGGFQHQEGGFRENRREGGF
                     QRREGGFGRDRREGGFQHQERGFRENRREGGFQRREGGFGRDRCGGGFQRREGGFRKP
                     GFGGRSSSSFSRGNRGH"
     gene            1451..2878
                     /locus_tag="PK077_06315"
     CDS             1451..2878
                     /locus_tag="PK077_06315"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN23952.1"
                     /translation="MKKHCRWWLFAGLWGLSLVQHPLLASQNISSPEPAGEGNFEAYL
                     SGLRERALNGDPAAARELAVHYDVEGNAAETSKWMSEYVSLAEKRANNGDVDSMLDLG
                     KLFYTGSRLYPKNLERARYWFTRAADSGNAAAQYQVAVMASQGAGGPKDEATAALYYK
                     KSLQTWKKEADDGDSKAALWAALVYERKLVPDSSPEKSVPYLLQAAESGNLTAQGLLA
                     FKYRDGLGVPQDAAKAVEWFEKAASRKDLGAVMELGIMFRDGKYLPPDREKAFHWFEK
                     GAEWKDPYSMAALADMLLEGTPSAEQAARALALYREAAAAGYFPAALKAAELLQNGKG
                     GELDADEAYRLLRRVADAAGDPKAMYMLAQVYYTRGDEAQGDSLMKASAQAAYLPAMN
                     RMARLHLLPDSSLPWNPVLSYYYWNQAGEMGDEKAASAAFWLLWGGSGIFLLAIFIIV
                     WRFQRFAARRLAEQQKQEREASDDA"
     gene            2936..3235
                     /locus_tag="PK077_06320"
     CDS             2936..3235
                     /locus_tag="PK077_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRN23953.1"
                     /translation="MMNMLAIFGLGTPEIIAILVIVFLLFGAKKLPEFARGLGKSLGE
                     FKKAKSEFEEELLKTEKETMSDASASKTEVRPSSELSQPIDVEVEKTVDSKPEDK"
     gene            3459..3740
                     /locus_tag="PK077_06325"
     CDS             3459..3740
                     /locus_tag="PK077_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23954.1"
                     /translation="MMEHVPCEGEVFDGCIFTETATRAGKKCNFMFTGILYRRRNGAV
                     YWFFSFYGLEKRKGMDFAADVSRLSKGILNERLQVLISCVARWLVVHMY"
     gene            3740..5146
                     /locus_tag="PK077_06330"
     CDS             3740..5146
                     /locus_tag="PK077_06330"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRN23955.1"
                     /translation="MKIHFCGAAGTTTGSQHLLEVNGCRILLDCGMYQGRREEAWHIN
                     KDFPYFSPAEVDAVVLSHAHIDHSGNLPNLVKQGFQGNIYSTFATRDLCQLMLADAAR
                     IQEHDCAFINKMNKRRGIHQPDVYPTYSEQDAERCMRQFVNIGYDRPIPVVPGVTLTF
                     YDAGHILGAAQVCLDIEDREDGRKKRFLFSGDVGRGDNELLRDPVPVPDVDILLMEST
                     YGGRFHEAPSRDDETFCRAIKEALELGGCVYIPAFAVERTQQLLYLLNRAYHEGTLPL
                     LPVYVDSPMAVGATEIFRIHPECFNKEVYDFLFREKDPFCFEQLRLIRSVGESQALNK
                     MNGQAIIISASGMCEAGRILHHLANNIGDPKNTVLFVGYCAEHTLGRKIMDGWKEVPI
                     LGKQYPVRARIREMDSFSGHADHGELLEYFDRTGGPKRNIILVHGEEKATQALAGALR
                     ERQANPVSIAQLGSAMVL"
     gene            5314..5673
                     /locus_tag="PK077_06335"
     CDS             5314..5673
                     /locus_tag="PK077_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HRN23956.1"
                     /translation="MSSLFIEYPKCTTCQKAKKWLDEQGISYEDRHIVEQNPTAEELR
                     SWIEGSGLPAKKFFNTSGLLYKSMKLKDKLADMSLDEQIALLASNGMLVKRPIFITNG
                     KICVGFKADEWKAAHRS"
     gene            5682..>5944
                     /locus_tag="PK077_06340"
     CDS             5682..>5944
                     /locus_tag="PK077_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23957.1"
                     /translation="MPQITRTKLGAANFGPWENIVRVLILVWFMLYTLNAIPDIHPLV
                     GRIAAKVNYWISYFFMFEYILRVLCAKPRKAYIFSGMGILDFIV"
BASE COUNT         1328 a         1338 c         1835 g         1443 t
ORIGIN      
        1 ccggtgagat agcggacatt ataccaatat gagtgaagaa caacatggct ccgaagaaaa
       61 cggaggtatc cgcattaata aattcctggc ctcctgcggc attgattccc gccgggtggc
      121 cgacaggctg attggagaag gacgtgtgga agtgaacggg aaggttatcg atacgcctgg
      181 gatgagggtg acggagaagg atttcgtgaa ggtggacggc cgccatatga cccctatgga
      241 agaggttgtg gtgctgttga acaagccccg cgggtatgtg tgcagccgcg aagcgcaggg
      301 agccataggt actgtttacg acctgctgcc accgcgcctg cgccatctga attatgtggg
      361 acggctggat gccgattccg aaggtcttct gattatgacg aataaggggg aattgaccca
      421 ggttctttcc catcctaccg gcggcataga aaaggagtac tgggtgacgg tagatcagaa
      481 ttttgataat tccgtactga tgcagttttt gcgcggagta cgcattccgg agggaaatgc
      541 caaggccaaa tatgtttgcc gcgcttccgc gcgccgtgcc tgcattgttc tggaacaggg
      601 gttgaagcgt caggtgcgtc aaatgttcca gtgcctgggc cttcgtgtcc gcaagttggt
      661 gcgcgtgcgc atcggctccc tgtggggagg ggacttggaa cccgggggct ggaaattcct
      721 ggatgatgcg gacgtggctc tgtcactgaa aaatcctccc cgccagcgta aatacctagg
      781 tgcttcccag cttgtcgccg gggggggcgc caagggtgag cagggcggaa gaggcgttga
      841 ttccgatgag gattatgtgt ttaatccgga agattttgaa gctggggatt cttatgaacc
      901 tactccggaa atgaagactc gttttgtgga gacggaggat gaacgtgaag tcaaggaaga
      961 tggatatttc cggggagatt ccggcttccg ttcccgtgac cgccgcggag attttcatcg
     1021 tcgtggagat ggcttccgcg gccgccgcgg ggatggggcc ctgcgccgcg aggccggttt
     1081 caaagaagag cgacgtaaag gagggttcca acgccgtgaa ggcggctttg gaagagaccg
     1141 ccgcgaagga ggttttcagc atcaggaggg aggtttcagg gaaaaccgtc gtgaaggagg
     1201 attccaacgc cgtgaaggcg gttttgggag agaccgccgc gaaggaggtt ttcagcatca
     1261 ggagagaggt ttcagggaaa accgtcgtga aggagggttc caacgccgtg aaggcggttt
     1321 tgggagagac cgctgcggag gaggttttca gaggcgtgaa ggcgggtttc gtaagccggg
     1381 ctttggaggc aggtcctcca gcagtttttc ccgcggaaac aggggccatt aatgaatgac
     1441 ttgttccggt atgaagaaac attgcagatg gtggcttttt gccggattgt ggggactgtc
     1501 cctcgttcag catccacttc ttgcttctca gaatatttcc tctccggagc ctgccgggga
     1561 gggaaatttt gaggcttact tgtccggtct ccgcgaaaga gctctgaatg gggatccagc
     1621 tgccgcccgg gagctggctg tgcattatga cgtggagggt aatgctgcgg aaacgtccaa
     1681 atggatgtcc gagtatgttt ccttggcaga gaaaagggcc aataacggag atgtggattc
     1741 catgctggat ttgggcaagc tgttttatac aggcagccgc ctgtacccaa agaacctgga
     1801 aagggcccgg tactggttta cccgggctgc tgacagcggt aatgccgcgg cacagtacca
     1861 ggtggctgta atggcttccc agggagcagg aggaccgaag gatgaagcaa cggcagccct
     1921 ttattataaa aaatctctcc agacgtggaa gaaagaggcg gatgacggtg attccaaggc
     1981 ggcgttatgg gctgcccttg tttatgaacg aaagctggtt ccggacagtt ctccggaaaa
     2041 gtcagtccca tatcttcttc aggcagcgga aagcggcaac ctgacagcac aaggccttct
     2101 ggcatttaag taccgggatg ggctgggagt gccgcaggat gcggccaagg ccgtagaatg
     2161 gtttgagaaa gcggcctccc gtaaagattt gggagccgtg atggaactgg gcataatgtt
     2221 ccgggacggc aagtatttgc cccctgaccg ggaaaaggcc ttccattggt ttgaaaaagg
     2281 ggcggaatgg aaggatccgt acagcatggc tgccctggcg gatatgctgc tggagggaac
     2341 tccttccgca gaacaggcgg cccgggccct ggctctgtat cgtgaggctg ccgccgccgg
     2401 ttatttccct gcggcactaa aggccgcgga gctgctccag aacgggaagg gcggggaact
     2461 ggatgcggat gaggcctaca ggctgctgcg gcgtgtggcg gatgctgcag gggatcccaa
     2521 ggccatgtac atgctggccc aggtatatta tacacggggt gatgaggctc agggagattc
     2581 cctgatgaaa gcatccgccc aggctgccta tttgccggcc atgaaccgca tggcgcgtct
     2641 ccatcttctg ccggacagtt cactgccctg gaatccggtt ttatcctatt attattggaa
     2701 ccaggctgga gaaatggggg atgaaaaggc ggcttccgcc gctttttggc tgttgtgggg
     2761 cggctcaggc atctttttgc tggcaatatt tattattgtc tggcgttttc agcgttttgc
     2821 cgccagaagg cttgcggaac agcagaaaca ggaacgggag gcctctgatg acgcatgaag
     2881 cgtattatga ggtatccggc atgaaaaaaa tctgttaaaa atactgtagt actccatgat
     2941 gaacatgtta gctatttttg gactgggaac accggagatt atcgccattc tggtgattgt
     3001 tttccttttg ttcggcgcaa agaaacttcc ggaatttgcc cgcggactgg gcaagagcct
     3061 gggagaattc aagaaggcca aatctgaatt tgaagaagaa ctgctgaaga ccgagaagga
     3121 gaccatgagt gatgcttccg cctccaaaac ggaggtccgg ccttcttccg agctttccca
     3181 gcccattgac gtggaggtgg agaagacagt ggactccaaa ccggaggaca agtaattttt
     3241 tgtaattgaa cggcattatg aacagggcgg gaatttcctg tcctgttcat tttagctgaa
     3301 aggcttcatt aagattaggc gggactcttt ttgccggccg cttttttcag gaggcgggtt
     3361 gcaatcagaa cggaatgttt tcagaataac ctgcaggttt tatttttggc agtgataccg
     3421 gagggctctc ctggatgcgg gtattccggc gtgcattcgt gatggagcat gtgccttgcg
     3481 aaggggaagt ttttgacgga tgcattttta cggaaaccgc aacccgggcg ggaaagaagt
     3541 gcaatttcat gttcacagga atactgtaca gaaggaggaa tggtgcagtt tattggtttt
     3601 tctcttttta tggcctggaa aaaagaaaag gcatggattt tgcggctgat gtttcccgtc
     3661 tttcaaaggg tatattgaat gaaaggcttc aggtattgat ttcttgcgtt gcccgctggc
     3721 ttgttgtaca catgtattaa tgaaaattca tttttgcggc gcagcgggaa cgacgacggg
     3781 ctcccagcat ttgctggagg tgaacgggtg ccggattctt ctggattgcg gcatgtatca
     3841 ggggcggcgt gaggaggcat ggcatatcaa taaggatttc ccctattttt ccccggcgga
     3901 ggtggatgcg gtagttctgt cccacgctca tattgatcat tccggcaatc ttcccaattt
     3961 agtgaaacag gggtttcagg gaaatattta cagtacattt gcaaccagag atctttgcca
     4021 gctcatgctg gcggacgccg cccgcattca ggagcacgac tgcgcattca tcaataagat
     4081 gaacaagcgc cgcggtattc atcagccgga tgtttatcct acttattccg aacaggatgc
     4141 ggagcggtgc atgcgccagt tcgtgaatat cgggtatgac cggcccattc ccgttgttcc
     4201 gggggtgaca ctcacgttct atgatgcggg ccatattctg ggcgctgcgc aagtgtgcct
     4261 ggatattgag gacagggaag acgggcggaa gaagcgtttc ctgttttccg gggatgtggg
     4321 gcggggagat aacgaattac tgcgcgatcc cgtccctgtt ccggatgtgg atattttgct
     4381 gatggagagc acttacggcg gccgtttcca tgaggccccc tcccgtgatg acgagacgtt
     4441 ctgccgggct atcaaggagg ctctggaatt aggcggctgc gtttatattc cggcttttgc
     4501 ggtggaacgc acccagcagc tgctttatct gctgaacagg gcctaccatg aaggcactct
     4561 gcccttgttg cccgtatatg tggacagtcc catggcagtg ggtgcgacgg agattttccg
     4621 cattcatccg gagtgtttca ataaggaggt ttatgatttc ctgttccggg agaaggatcc
     4681 gttttgtttt gaacagctgc gtttgatccg ttccgtgggc gagtcccagg ctttgaataa
     4741 aatgaacggg caggctatta ttatttccgc ctcaggaatg tgtgaagcag ggcgcattct
     4801 tcaccacctg gcgaataaca tcggcgatcc gaaaaatacg gttctttttg taggctattg
     4861 tgcggaacat acacttggcc gcaagattat ggatgggtgg aaagaggttc ccatcctggg
     4921 taaacaatat cctgtccggg ccagaatacg ggagatggat tccttttccg gccatgcgga
     4981 ccatggagaa ctgctggagt attttgacag gaccgggggg cccaagagga atatcattct
     5041 ggtgcatggg gaggagaagg ctacccaggc tctagcgggg gcgctccggg agcgtcaggc
     5101 caatcccgtt tccatcgcgc agttgggaag cgccatggtg ctttaatgtt ccggaatata
     5161 gaatttctgc ggtatccgca cgcattccgg gctttcgttc tcaggttttg tgttcaacgg
     5221 cggatacatc ggcagccttc caatcaagtc atgacgtcgg gatggctgcg ctggccgctt
     5281 gacccggtgt gtttacgggg ctagcgtgcg gatatgtctt cattatttat tgaatatccc
     5341 aaatgcacga cctgccagaa agcaaagaag tggcttgatg agcagggaat ttcttatgag
     5401 gatcgccata ttgtggaaca gaatcccacg gcagaggaac tgcggagctg gattgaaggg
     5461 agcggattgc cggccaagaa gttttttaat accagtggat tgctttacaa gagcatgaag
     5521 ttgaaagata agcttgccga catgagcctg gatgagcaga tagccctgct ggcttccaac
     5581 ggcatgctgg ttaaacgccc catttttatt accaatggaa agatatgcgt cggcttcaag
     5641 gctgacgagt ggaaggccgc ccatcgttcc tgattcccgc catgccccaa attacccgca
     5701 ccaagctggg cgctgccaat ttcggtccct gggagaacat tgtcagggta ctgattctgg
     5761 tatggttcat gctgtacacg ttgaatgcca ttccggatat tcatcctctg gtaggccgta
     5821 tcgcggccaa ggtaaattac tggatcagct atttcttcat gtttgagtat atcctgcggg
     5881 tgctgtgcgc caaaccgcgg aaggcttata ttttcagcgg catgggtatt ctggatttta
     5941 ttgt
//
LOCUS       DAOMSU010000122         5942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1137_length_5942_cov_3.261593, whole genome shotgun sequence.
ACCESSION   DAOMSU010000122 DAOMSU010000000
VERSION     DAOMSU010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1137_length_5942_cov_3.261593"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..389
                     /locus_tag="PK077_06345"
     CDS             192..389
                     /locus_tag="PK077_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23958.1"
                     /translation="MFKYILNRLTERSTWLGLLALATACGATIEQALADQIIAAGMAV
                     AGLIGIVTKDRKKEQENHDNG"
     gene            382..969
                     /locus_tag="PK077_06350"
     CDS             382..969
                     /locus_tag="PK077_06350"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRN23959.1"
                     /translation="MADSISDLQKDTFYWQRLLRLAGYYHGAIDGIPGNGTRNGTERW
                     STDADRYKMETGCFDERTERNISTLLPEAQKAARQWFKLARNEAVNQGYEAKIICGTR
                     TYAEQNDLYRQRPKVTNARGGQSWHNFGLAWDFGIFQDRNYLPNHPLYTTLGKLYAKI
                     DGLEWGGTWKSFTDPPHLQLHQFGSISEARRSFET"
     gene            983..1321
                     /locus_tag="PK077_06355"
     CDS             983..1321
                     /locus_tag="PK077_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23960.1"
                     /translation="MTQTAVTTSVVLANTLAVVCMATSELNPLLEYVQNGASVAAVMG
                     IFLWREMKRAERYEKLYDQERRQRITAERKCSSCMFMKLAHEQFAGIHSQKGNAPSPE
                     NHRRSLTTEQ"
     gene            complement(1431..1517)
                     /locus_tag="PK077_06360"
     tRNA            complement(1431..1517)
                     /locus_tag="PK077_06360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1481..1483),aa:Leu,seq:cag)
     gene            1718..3517
                     /locus_tag="PK077_06365"
     CDS             1718..3517
                     /locus_tag="PK077_06365"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HRN23961.1"
                     /translation="MNETNEKPLLNRFEVMEMGFGLCTLAALMWLLWWNGFGEIHEDQ
                     LSLRYFREILSFLVLGQLTGVIGILREKWLARAIRNPRWVELGLGLVILVMLAGDFFH
                     STFISKIELQNIFRALCLCYGAAPLLNLKEYFFRIARRNRGFHLPRIRPALLFLLTLI
                     AFIALGGTLLMSPGATKDGIVLSATDAYFISTSAVCVTGLVPLNVHEHFTNYGQTILL
                     VLFQVGAFGIMTFTYFVSLMVGQGLSLRSKVTISNLLDEEGIAQVDRFIKNIIAVTFG
                     VEALGAIGLYFSWRHVPGLEGDTLWWYAVFHSVSGFCNAGFSLFADNLATPGVAYNMG
                     GQITIMVMVLCGSLGFAMYLEIVRRARVALGWDARNTVSRHWSTYSWLVVRMTAALVL
                     GGGLILFLIKIIDGSLFTSADPWYWILWESLFNATARTAGFNISDMALNSAPYALFLG
                     ALMFIGGNPGSTTGGVHTTAFAVSCGEVARILQGKKDVVMHKRRIARNVVERAVITVI
                     LAGAWVGMMTTVMCFAEPRLSLERLFFEVVSSFATVGFSWNVTPELSDAGKWIIVFNM
                     IVGRVGMVAFVLAFMKQPTKSPLRYPETRLPLS"
     gene            3697..4992
                     /locus_tag="PK077_06370"
     CDS             3697..4992
                     /locus_tag="PK077_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRN23962.1"
                     /translation="MSYPLMISLIIEHMIGLTDAVFLGRVGDVALGACALGGVYYMAM
                     FMLAFGFGFGAQIIIARRNGERRYNRIAPTMVQGCYFMLVLAVALFAASQYFSPIILR
                     DIIESPQVYGATADYLHWRTYGIFFSFLCVMFRAYYVGITQTKILTVNSIVMLLSNVA
                     LNYCLIFGKGGFPAAGIAGAAIASSVSEAVSLLFFIIYTSMTADKRKYGFRRAFTLRP
                     HLLKGMLSISGWTMVQSFISVSIWFIFFLAIEHLGERPLAVTNIVRNISALLIMVISA
                     FATTAGALISNQIGAGEQRCLFKTCGMTLKLTYAILIPLLIILCLFPESVLRAFTDSP
                     SLIAASVNSVYVMASSTLIMAPSFILFNAVSGTGNTRAGFIMEMISLAVYTAVIYYGI
                     IRLKPDVALCWFSEHVYGVTLIFLAWWYLKSGKWRGKKV"
     gene            complement(5248..>5942)
                     /locus_tag="PK077_06375"
     CDS             complement(5248..>5942)
                     /locus_tag="PK077_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23963.1"
                     /translation="VADAASVEAPKTKIKTVKSAPKTAVKTKTAKTAKKAADEKAPVS
                     ETVVAPSEAPAPSAPVEKEMMPEAPQKPQAAAPVKVKRESFRPNRNAEQQERHEQHGG
                     DVFAQPETVGGASEGPFNKRKRRRRNKKGGGSEDRQPMQDAASLKQLDCKKVASRAWK
                     MFLAEVSEEGLALMDDQTAREAARRAFRCAEFFMMEEARRKHAQKAALQQQEKTSSPE
                     EERDSEEDSGEE"
BASE COUNT         1272 a         1731 c         1544 g         1395 t
ORIGIN      
        1 aaaagtatcc gcggaatctt tgcaaatcgc ggcagtaaac tcagaacaac ttgtgcggag
       61 aactgatagc cccactttcc ccattggtgg agaggattcg gaaaaacctt ttccccatcc
      121 tttggcatcc ccggaaaggc aggttccccc ttctcccaca gagggacttt tcaataccaa
      181 ccgaccatca catgttcaaa tacattctca atcgactgac ggaacgttcc acatggctgg
      241 gcctccttgc cctggccaca gcctgcggag ccactatcga acaagctctt gccgaccaga
      301 ttatcgccgc aggcatggcg gtggcaggcc tcatcggcat tgtcaccaaa gaccggaaaa
      361 aggaacagga aaaccacgac aatggctgac agcatttccg acctgcagaa agataccttc
      421 tactggcaaa ggctcttgcg cctggccggg tactatcacg gagccattga cggcattccc
      481 ggcaacggca cgcggaacgg aacggaacgc tggagcacgg acgccgaccg gtacaaaatg
      541 gagaccggat gctttgacga acgcaccgaa cggaacatct ccacccttct tccggaagcg
      601 caaaaagccg cccggcagtg gttcaaactg gcccggaatg aagccgtcaa ccagggatat
      661 gaagcaaaaa ttatctgcgg aacgcgcacg tatgccgaac agaatgacct ctacaggcaa
      721 cgccctaaag taacgaatgc ccgcggcggc cagagctggc acaacttcgg cctggcctgg
      781 gattttggaa ttttccagga ccgcaactac ctgcccaatc accctcttta caccactctt
      841 ggcaaacttt acgcaaaaat tgacggcctg gagtggggag gaacatggaa atcctttaca
      901 gatccgccgc atttgcagct ccatcaattc ggaagcattt ccgaagcgcg cagaagtttc
      961 gaaacatgac ctttccagcc ccatgaccca gactgccgtt actacaagtg tcgttttagc
     1021 caacaccctt gcggttgttt gcatggcgac ttcagaactc aatcccctgc ttgaatacgt
     1081 tcaaaacggc gcctccgtgg cagccgtgat gggcatcttc ctctggaggg aaatgaaacg
     1141 cgccgaacgc tacgagaaac tttacgacca ggagcgcaga caacgcatta ccgctgaaag
     1201 gaaatgttcc tcctgcatgt tcatgaagct ggcgcatgaa caattcgcag gcatccactc
     1261 ccaaaaaggc aacgccccct cccctgaaaa ccacaggcgt tccttaacaa cggagcaata
     1321 atccttatcc agccccttca ccaatcttcc ggcgttgcgt caaagccaat gcacgaatac
     1381 atcataaaac cagtaaggcc ctgcaagcag ttaacttgca gggccttgtt tggtgcgccc
     1441 gaaaggactc gaaccttcac gggggaaccc ccactagaac ctgaatctag cgcgtctacc
     1501 aattccgcca cgagcgcaca tggtttggta agaacggggc ttattatgca cggcggcgcc
     1561 ggattggcaa ctattttcct tcacatgaaa aaaatttttt cccaaggcac cttcctgcct
     1621 tcagccgggg ccgcctggaa acgatataag ggcttctcaa atctttccgt atgcagtagt
     1681 ctccccccag catcagaggc atcaccattg caggcttgtg aatgagacga atgaaaaacc
     1741 gctattgaac cgttttgagg tcatggaaat ggggttcggc ctgtgcacac tggccgcatt
     1801 gatgtggctg ctgtggtgga acggcttcgg ggaaattcat gaagaccagc tttccctgcg
     1861 ctatttccgc gaaattctct ccttcctggt gctgggccag ctcacgggcg tcatcggcat
     1921 tttgcgggaa aaatggcttg ccagggccat ccgcaacccc cggtgggtag aactggggct
     1981 ggggctggtg attctggtca tgctggcagg cgactttttc cattccacct ttatctccaa
     2041 aatagagctt cagaacattt tccgcgcgct ctgcctctgc tacggagccg cccctctgct
     2101 gaacctcaag gaatattttt tccgcatcgc ccgccggaac aggggcttcc acctcccgcg
     2161 cattcgtccc gcccttcttt tcctgcttac cctgattgcc ttcattgcgc tgggaggaac
     2221 tcttctcatg tcgccgggag ctaccaagga cggcatcgtc ctttccgcca cggacgccta
     2281 tttcatcagc accagcgccg tctgcgtcac ggggctggtc cccctgaatg ttcatgaaca
     2341 cttcaccaat tacggacaga ccattttgct ggtgcttttc caagtgggag ctttcggcat
     2401 catgacgttc acttatttcg tctccctgat ggttggccag gggttgagcc tgcgttccaa
     2461 ggtaaccatc agcaacctgc tggatgaaga aggcattgcc caggtagacc ggttcatcaa
     2521 gaatattatt gccgtcacgt tcggagtgga agcgctggga gccataggcc tctatttttc
     2581 ctggagacat gttccggggc tggagggaga cacgctctgg tggtacgccg tcttccattc
     2641 cgtttccggt ttctgcaatg cgggattcag cctgtttgcg gataacctgg ccacaccggg
     2701 cgtagcctac aatatgggcg gccagattac cattatggtc atggtgcttt gcggcagcct
     2761 gggattcgcc atgtatctgg aaatcgtccg ccgggccaga gtcgcactgg gctgggatgc
     2821 ccggaacacc gtcagccgcc actggtccac atacagctgg ctggtcgtac gcatgaccgc
     2881 cgctctggtg cttggcggcg ggctgatcct gttcctgatt aaaataattg acggcagcct
     2941 gttcacgtct gccgatccgt ggtactggat tttgtgggag agcctgttca atgcaacggc
     3001 ccgcacagca ggattcaaca tttcggacat ggcgctcaac agcgctccat acgccctgtt
     3061 tctgggagct ctcatgttca tcggaggcaa tcccggttcc accacgggag gagtccatac
     3121 caccgcattt gccgtttcct gcggggaggt ggcgcgcatt ttacagggca agaaagacgt
     3181 ggtcatgcac aagcgccgga ttgcccgcaa tgtggtggaa cgggccgtca tcaccgtcat
     3241 tctggccgga gcctgggtag gtatgatgac tacggtcatg tgttttgcag aaccgcggtt
     3301 gagtctggaa cgcctgtttt tcgaggtagt cagctctttc gccacggtag gcttttcatg
     3361 gaacgtcact ccggaactca gtgatgccgg caaatggatc atcgtgttca acatgattgt
     3421 gggccgtgtg ggcatggtgg ctttcgtgct ggccttcatg aaacaaccaa ccaaatcccc
     3481 cctgcggtat ccggaaacca gactgccgct cagctgaccc ggggcattac ccccattttc
     3541 atgacatgtg cggaagcaaa cgcatttctt tcttgcaaga aaacatttta attcaagaat
     3601 atttggcctt tagcgcctgc ccttggcgga tcatgcccgc aggctgcccc cgctatgaat
     3661 aatccttctt ttacttccta tcaaaagata tggctcatgt cataccccct catgatcagc
     3721 ctgatcattg agcatatgat cggcctgacg gatgccgtct tcctggggcg cgtgggagac
     3781 gtggcgctgg gagcatgcgc tctgggcggc gtgtattaca tggccatgtt catgctggca
     3841 ttcggcttcg gctttggcgc acaaatcatc attgcgcgcc gcaacgggga acgccgttac
     3901 aacaggattg cccccaccat ggttcagggc tgttatttca tgctggtcct ggcggtagcc
     3961 ctttttgccg catcccaata tttttccccc atcatcctgc gggacatcat tgaatcccca
     4021 caggtgtacg gggccacggc ggattacctg cactggcgca cctacggtat ctttttctct
     4081 ttcctctgcg tcatgttccg agcctattac gtggggatta cccagacgaa gattctgacg
     4141 gtcaattcca ttgtcatgct cctgagcaat gtcgcgttaa attactgcct cattttcggg
     4201 aaaggcggct ttcccgccgc aggcatagca ggggcggcca ttgcctccag cgtatcggaa
     4261 gcagtctccc ttctcttttt catcatctac acaagcatga cggcggataa acggaagtac
     4321 ggcttccgcc gggcattcac actccgcccc catctgctga agggaatgct gtccatttcc
     4381 ggatggacca tggtgcaatc ttttatttcc gtctccatct ggtttatttt tttcctggcg
     4441 attgaacatt tgggggaacg cccgctggcc gttaccaaca tcgtccgcaa tatttccgcc
     4501 ctgctgatca tggtgatcag cgcttttgcc accacggcag gagctctcat cagcaaccag
     4561 attggagcgg gagaacagcg ctgcctgttc aaaacctgcg gcatgactct caaactgaca
     4621 tacgccatcc tgatccccct gctcatcata ctgtgcctgt ttccggagtc cgtcctccgt
     4681 gccttcacgg atagtccgtc cctgattgcc gccagcgtca attccgtgta tgtgatggct
     4741 tcttccacac tgatcatggc tccgtcattc atcctgttca atgccgtatc cggcacaggc
     4801 aataccaggg cgggattcat catggaaatg atttccctgg ccgtttacac ggccgtcatt
     4861 tattacggca tcatcaggct caagccggat gtggctctct gctggttttc cgaacacgtt
     4921 tacggcgtta ccctgatctt cctggcatgg tggtacctca agagcgggaa atggagaggc
     4981 aaaaaggttt aggaggaaca agaacgggga acggcacgaa ataaagtccg ctttcatcca
     5041 agcggctcca acgctctccc attctcaaaa agtgccctgg ttggagaaaa ggctgctttt
     5101 tgcccccagg tggcaaaggg cggcgcatgc tgcttcccaa tgcaccggat aaaacacggc
     5161 cattcaaact ccggaaaata agcccggagg atacaaaaac gccctgcggc atccgccgca
     5221 gggcaaaaac atcaacactg acaggaatta ttcttcgccg gaatcctctt cggaatcccg
     5281 ttcctcttcc ggagaagaag ttttctcctg ctgctggaga gctgctttct gggcatgctt
     5341 gcggcgcgct tcctccatca tgaagaattc cgcacagcgg aaagcgcggc gggccgcctc
     5401 acgcgccgtc tggtcgtcca tcagggcaag gccttcttca gacacttccg ccaggaacat
     5461 tttccaggct cgggaagcca ccttcttaca atccaactgc ttgagagaag cggcatcctg
     5521 catgggctgg cggtcttcgg aaccgccgcc tttcttattg cggcggcggc gcttgcgctt
     5581 gttaaagggc ccttcggagg cgccgcccac cgtttccggc tgggcaaaga catctccgcc
     5641 atgctgttca tggcgctcct gctgttccgc attgcgattg gggcggaacg attcccgctt
     5701 gaccttgacc ggagcggcag cctggggctt ctggggagct tcgggcatca tctccttttc
     5761 cacgggagcg gaaggagccg gggcctcgga aggggcaaca accgtttcag agacaggggc
     5821 cttttcatca gccgcctttt tggctgtttt cgcggttttt gtcttcaccg cggttttcgg
     5881 ggcggacttg accgttttga ttttggtttt gggtgcttct acggaagcgg cgtcagcaac
     5941 ag
//
LOCUS       DAOMSU010000123         5854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1165_length_5854_cov_2.923780, whole genome shotgun sequence.
ACCESSION   DAOMSU010000123 DAOMSU010000000
VERSION     DAOMSU010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1165_length_5854_cov_2.923780"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="PK077_06380"
     CDS             <1..1080
                     /locus_tag="PK077_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HRN23964.1"
                     /translation="AGVREGRRVLKPLSELWGEGNPWGDVLSGWIPDRELLCSRRNGP
                     ASQLALLLARQAVEDAGWGRKELRDAALIVGSSRGNASGWLSPWPGRRPMKILAVPNS
                     LHSELASCVSIELGICGPYHVLASGCAAGLDAVGMAAMLMRQGVVKRALAIGLDLPLC
                     RELLGTYWASGMLSRNGLNDPYGPLADGMCISEGGAAIALELSSAPGIYVKDYLVNSD
                     AYSPLGMPEDGKSIAALLEAALKSRDGAVPLTVCPHASGTAGNSVSERAALKRVFKDG
                     LPDLRMMKPWTGHAIGGSGILELALILVFAREGVLPPNPPWATFPEGGACSAEELPLA
                     GGRMLVKSAASMGGHNVVLSLAVDG"
     gene            1104..2267
                     /locus_tag="PK077_06385"
     CDS             1104..2267
                     /locus_tag="PK077_06385"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HRN23965.1"
                     /translation="MKNPEQFLSELKSAGLLRVLRDVECLPGGMARLADGRKVVNLAS
                     NDYLGLAHHPSLAEAFSRAVRDEGAGAMASRLVTGTRREHSCLEEALAALKGTEAAVS
                     FSSGYATSLGVIASIADREDTVLMDKLSHASLIDGARLSGARLSTFLHNDMESLRKKL
                     QHLRNANPSGGILVVTESVFSMDGDRAPLREIVRLKDEFGALLLVDEAHGFGVLGEHG
                     AGLAEELGVSSRIDFQMGTLSKAAGVSGGYVACSRAWADVMVNSARSLIYSTAPPPAL
                     AAAALAAVEVIRSGEGKELRRRVSVLANSLSAALGMPGRPVSSIFPVVVGENDAALAA
                     ADALLEQGFLAPAIRYPTVPRGTARLRITVTAAHESGQVTRLGTALKELLHGK"
     gene            2520..2975
                     /locus_tag="PK077_06390"
     CDS             2520..2975
                     /locus_tag="PK077_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23966.1"
                     /translation="MMNTSDSSSPSSCCSGSPSCTAALVLGIIALVLALIGFFPCLGW
                     ITLIPAGLLAIIALIMSIVAVSKGSPKGALVVAIIALVLSAVAGIVQAWMASATVTYM
                     EQHQDYVEKKALEIQKELRQMELENREPLRQSGEADGMSPTDGTGSQDL"
     gene            3893..4444
                     /locus_tag="PK077_06395"
     CDS             3893..4444
                     /locus_tag="PK077_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23967.1"
                     /translation="MSIYRSIELRKNTSGSYKVRLTIDRGSKFCGARKSLGLGTHRED
                     CALRRAYLMRCLLFHMGWFPGGKIAVRNSAECGIKDVVWDSMECSEDSGMFKLAGRGC
                     RMAADETMAIWFQTNGDGRVQCLRLLISGWRKDDRAGDLRVLIQGMRHCASEQRQHPC
                     RILNIEIGAGSFTAPRDECVLKG"
     gene            complement(4725..>5854)
                     /gene="aspS"
                     /locus_tag="PK077_06400"
     CDS             complement(4725..>5854)
                     /gene="aspS"
                     /locus_tag="PK077_06400"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRN23968.1"
                     /translation="RCFRDEDLRADRQPEFTQVDIEASFITPEDIYNLVEGLLKRVYK
                     ESLGVDIPTPFPRMTWKEAMDQYGSDKPERRFGMKLTDVSSIFENSGFKVFASAVSNG
                     GVVKAINAKGFGSASVGQIDALTKTAVEAGAKGLAYIKVREEDWRSPISKFLSDEEKQ
                     KLTEALDIETGDLVLFAAGPWEPSCDILGRVRLQCAEFMELLKDNKERDFLWVIEFPL
                     VGWDEEEQRWVAIHHPFTRPVKEDEQKLLSGELSADLRAQAYDVVLNGTELGGGSIRI
                     HERDLQSAMFKALGITEEQAREQFGHILDAFSFGAPPHGGLALGLDRLVMMICNAESI
                     REVIAFPKNNRGADLMSDSPAAAEDRQLRDIHIQVKLPAKK"
BASE COUNT         1089 a         1389 c         1921 g         1455 t
ORIGIN      
        1 gccggggtaa gggaagggcg cagagtgttg aagccgttga gcgaattgtg gggcgaaggt
       61 aatccctggg gcgatgtatt gagcggatgg attccagaca gggaattgtt atgcagccgc
      121 aggaacggcc ctgcttccca gctggcgctg ctgttggcgc gccaggccgt ggaggacgcc
      181 ggatggggaa ggaaggaatt gagggatgcc gccctgattg tgggcagttc ccgcgggaat
      241 gcttccggat ggctgagccc gtggccggga cggcggccca tgaaaatttt ggccgttccc
      301 aattccctgc atagcgaact tgcttcctgc gtcagcatcg aattgggaat ttgcgggcct
      361 tatcacgtgc tggcgagcgg ctgtgccgcc ggcctggatg ccgtaggcat ggccgccatg
      421 ctgatgcggc agggagttgt taagagagcg ctggctatcg gtctggattt gcctttgtgc
      481 cgggagctgc tggggacgta ctgggcctcc ggcatgcttt cccgcaatgg tttgaatgat
      541 ccctacggcc ctctggccga cggcatgtgc atttccgagg gaggggcggc catagccctg
      601 gaattgtctt ctgctccagg tatttatgtg aaggattatc tggtgaattc cgatgcctat
      661 agcccgctgg gcatgccgga ggacggaaag agcatagccg ccctgctgga ggctgctttg
      721 aaaagtcggg atggagcggt tcctctgacc gtttgtcccc atgccagcgg cacggcgggg
      781 aatagcgtgt ccgaacgggc tgctttgaaa agggttttta aagacggact gccggatttg
      841 aggatgatga agccgtggac ggggcatgcc attggaggca gcggaatttt ggaactggcc
      901 ctgattctgg tctttgccag ggagggggta ttgcctccca atcctccctg ggccaccttc
      961 ccggaaggcg gcgcctgttc tgcggaggag ctgccgttgg ccggggggcg gatgctggtc
     1021 aaatccgccg cttccatggg cggccataat gtggtgctga gtctggctgt ggacggttga
     1081 atattgttga atagtgaacg cttatgaaga atcctgaaca gtttttgtca gagttgaagt
     1141 ctgcggggct gctgcgcgtc ctgcgggatg tggaatgcct gcccggagga atggcccgtc
     1201 tggcggatgg ccggaaagtg gtcaatctgg caagcaacga ttatctgggg ctggcccacc
     1261 atccgtccct ggcggaggct ttttcccgtg cggtgcggga tgaaggggcc ggagccatgg
     1321 catcccgcct ggtgacgggg acgcggcggg aacattcctg tctggaagag gccctggccg
     1381 ctttgaaggg gactgaagcg gcggtcagtt tttcctccgg ttatgctacc tccctgggag
     1441 tgattgcttc tattgcggac cgggaggata cggtgctgat ggataagttg tcccatgcca
     1501 gcctgattga cggggcgcgt ttgtccgggg cgcgtttgtc tacgtttctg cataacgata
     1561 tggagtcatt aagaaaaaaa ctgcagcatt tgcggaacgc gaatccttcc ggcgggattc
     1621 tggtagtgac cgaatccgtt ttcagcatgg atggagaccg tgcccccctt cgggaaattg
     1681 tgcgtctgaa ggatgaattc ggcgcgttgc tgctggtgga tgaagctcac ggattcggcg
     1741 tgctgggaga acacggggcc ggactggcgg aagaactggg cgtttcctcc cggatagatt
     1801 ttcaaatggg gactttgagc aaggctgccg gggtaagtgg tggatatgtg gcctgttccc
     1861 gcgcctgggc ggatgtcatg gttaattccg cccggtccct gatttattcc acggctcctc
     1921 ctcccgcttt ggccgctgcc gccctggccg cggtggaagt gatccgcagc ggggagggaa
     1981 aggaattgcg ccgccgtgtt tccgtgctgg caaatagttt gtccgccgca ctggggatgc
     2041 cgggaaggcc tgtttcttcc atcttccctg ttgtggtggg agagaacgat gctgccctgg
     2101 ctgcggcgga tgccctgctg gaacagggct ttctggctcc tgccatccgt tatcctacgg
     2161 tcccccgcgg aacggcccgg ttgcgcatta cggtaacggc tgcgcatgaa tccggacagg
     2221 tcacgcggct gggaacggcg ttgaaggagc tgctgcatgg gaaataacgc aggagttgtt
     2281 tttggttctc cgcattagag ccggttcctg tttttatgcg gggtctgccg gaaatggggt
     2341 tatcctcatt gatgctgcca taaaagggga agaaggatat ctgatgcctg gcggaacgga
     2401 atgacatccg gatgtcctga atggcagagc ttgcatcttg acaggaaagt tttcttccag
     2461 taaggggaaa gaatgaaggt tcccaaagga attttcactg aaccctataa aataacgtta
     2521 tgatgaatac ttctgattct tcttctcctt cgtcctgctg cagcggttct ccttcctgca
     2581 cggccgctct tgttctcggc attatcgccc tggtattggc attgatcggt tttttcccgt
     2641 gcctgggctg gattactctg attcctgcgg ggctgcttgc cattattgct ctgatcatga
     2701 gcattgtggc tgtcagtaaa ggttcaccca aaggggctct tgttgttgcc attatcgcgt
     2761 tggtcctgtc cgccgttgcg ggaattgtcc aggcatggat ggccagcgct accgtgactt
     2821 acatggaaca gcaccaggat tatgtggaaa agaaggctct ggaaattcaa aaggagcttc
     2881 ggcagatgga gctggaaaac agggagccat tgaggcagtc cggggaggcg gatggaatgt
     2941 cccccacgga tggaacaggc tcccaggatt tgtagttcct gaaccttttt aggaagatcg
     3001 gcgatgaatg gcgggcgctc ttcatggcgt tcatgccgtg atagctgggt ggtcaggaaa
     3061 gtatggctgc ctgtatgggc tgttctgtcc tgcctgttgc tggcgccctc cctcatggcg
     3121 ctggcaatta gccctgacag gatgaattct ctggcggctt ctgttgccgt gccgcatcgt
     3181 gaatgcccgt tgtgcggcat gacgcgaggg tatgcggaaa tggcccgcgg ggatatacga
     3241 gctgcctggg aatggaaccg cggagctccg gtcttgttta tggcaggact gctcaatgga
     3301 ggggctgccg cgttttattt ggcatggtgc tggcggagaa gggcgggaag ccgggaaaag
     3361 gaaaacagtt ctctcgcgta gcatgccgca aggggaggaa ccggctcccc ttgtccctgg
     3421 taaatgacac agcggaagtt ttgaagcggg gcctgggaag tttttctgcc tcctgtttat
     3481 tccctgtaac ggggctggcg cttggtggtg gggggacgga atgatgttga gtttttattg
     3541 aatgaattcc atgccaaatt cctcgtagtg ccgaagatga agttccactg cccaggttga
     3601 gcgttttgcc ggagggagct gcgttgttcc tgaaaaggag aggggaatgc ggcgctggcg
     3661 tttcatgcct taggattatt ttttgtcaga aaggggaata gcttttctgg gaacggccgg
     3721 tattgccgtt catggaagga gtatcctggg tgtgaaatat tttgattttt ctttatttag
     3781 gtattttgtt gatgatgttg ttttatatta agttgtgtaa ttttctaaca tttttattct
     3841 aggcccgttt gattttatat atatagtttt tggatattgt atgaatattt gcatgtccat
     3901 ctatagatca atcgaattgc ggaagaatac atccggaagt tataaggtcc ggctgactat
     3961 cgaccgggga agcaagttct gcggcgccag aaaatctctt ggtctcggca cccatcggga
     4021 ggattgcgcc ttaagacgtg cctatctgat gcgatgcctg ctgtttcata tgggatggtt
     4081 tcccggcgga aagattgccg tcaggaattc agccgaatgc ggtatcaagg atgtggtttg
     4141 ggacagcatg gagtgttcgg aggactccgg aatgttcaag cttgccggaa ggggctgccg
     4201 catggctgcg gatgaaacca tggctatctg gtttcagacg aatggagatg ggagggttca
     4261 atgccttcgt ttgctcattt ccgggtggag gaaagatgac cgggcaggtg atttgagggt
     4321 gttgattcag gggatgcggc attgcgcctc ggaacagcgc cagcatccct gccggatttt
     4381 gaatattgaa atcggtgccg gctctttcac ggcgccccgg gatgaatgtg tgctgaaagg
     4441 gtaggaaaac ggattgtttc agataccggt ttattctggt tttattcgga ggccgccaca
     4501 ttccggttgg gaaattgggc cgaatgtccg gcatgcccgg tccggagttt atttggccgt
     4561 tatggacgac gggaaggttg ccgtatcccg ggcgagttga aggcgcggga agaaggccgg
     4621 gaggaatggt gtgctgcctg ccgggaaagg gagccctgtt ttttctgcat gaaaaaaccc
     4681 cggatggaat gattgccatc cggggttttg tttgaagatt ccggctattt tttagcgggc
     4741 agtttcacct gaatgtgaat gtcgcgcagt tgccggtctt ccgcggcggc gggggagtcg
     4801 ctcatcagat ccgctccccg gttgtttttc gggaatgcga tgacttcgcg aatggattcc
     4861 gcgttgcaaa tcatcatgac caggcggtcc agtcccagcg caaggccgcc gtggggcgga
     4921 gcgccgaagc tgaaagcgtc cagaatgtgg ccgaattgtt cgcgggcctg ttcttccgtg
     4981 atgccgagtg ctttgaacat ggcggactgc aggtcgcgtt catggatgcg gatggagccg
     5041 cctcccagct ccgtgccgtt gagcaccacg tcgtaggcct gcgcgcgaag gtctgcggag
     5101 agttccccgc tgagcagttt ttgttcgtct tccttgacgg ggcgggtaaa ggggtggtgg
     5161 atggcgaccc agcgctgctc ttcctcatcc cagcctacca gcgggaattc aataacccac
     5221 agaaagtcgc gttccttgtt gtccttgagc agttccatga attcggcgca ttgcaggcgc
     5281 acgcggccca ggatatcgca ggagggttcc cacgggccgg cggcaaagag aacgaggtct
     5341 cctgtttcga tgtccagggc ttccgtgagt ttttgctttt cttcgtcgga aaggaatttg
     5401 gagatggggc ttctccagtc ttcttcgcgc accttgatgt aggccaggcc tttggcgccg
     5461 gcttccacgg ccgttttcgt cagggcgtca atctggccaa cggaggcgga accgaatccc
     5521 ttggcgttga tggccttgac tacgccgccg ttgctcacgg ccgaggcgaa taccttgaag
     5581 ccgctgtttt caaagatgga ggagacgtcc gtcagcttca tgccgaagcg gcgttccggc
     5641 ttgtcggaac cgtactgatc catcgcttcc ttccaggtca tgcgggggaa gggggtggga
     5701 atgtccacgc ccagggattc cttgtacacg cgtttgagca ggccttccac caggttgtag
     5761 atgtcttccg gcgtgatgaa ggaagcttca atgtccacct gggtgaattc cggctggcgg
     5821 tcggcgcgca ggtcttcgtc acggaagcag cggg
//
LOCUS       DAOMSU010000124         5822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1178_length_5822_cov_3.081325, whole genome shotgun sequence.
ACCESSION   DAOMSU010000124 DAOMSU010000000
VERSION     DAOMSU010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5822
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1178_length_5822_cov_3.081325"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="PK077_06405"
     CDS             complement(<1..1379)
                     /locus_tag="PK077_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23969.1"
                     /translation="MPPPAADAPCPEQAFNATALKWHTCAWLLLPVLVFLAGWMHWYA
                     ALPLTLLTAAGLAPRKRKEPQTRDSLPAFPLFTRSSFLALAAFAALMALSGWGEWVNQ
                     HPDHIVRNACLRELVASPWPVIFPDGNVLIYNTGFWLVPALAGKLAGLDAARMLVVLW
                     GAWGLFLSWLWLCVFSGRRSLLLALLMAAFGSLLNLQCWLGLNLFRLHYFGTAEQIMC
                     SANASIPVLLFFIFLASGRMPLYWMPILFAATAFYSPLAALGGLPLAACQWRRQWKGV
                     LPLRDVLLHPSFSAAVLLGTCFLLYYGAVNAPSSHMGMRPFYTRPGDFLLMATSFLLY
                     ALFCWRDFQRNPLFICTLATGLILPFFYVNGDVNDLLCKGSVPAMACLLTFLASSWTR
                     RPGFRVWIWLLLAFGMAPRFNIPYYCCSSSAFQAFLAPPASETEPSSFVSREWKQLFY
                     APQARRQDE"
     gene            1534..1806
                     /locus_tag="PK077_06410"
     CDS             1534..1806
                     /locus_tag="PK077_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRN23970.1"
                     /translation="MNKTQFIRELQRELGKDVTSRQAEHALNAVLETIARSVRRRSLV
                     KFRGFGTFRVKRRRARIVRHPENGGRLLVHESKTMKFRPSSLLWKE"
     gene            1936..4089
                     /locus_tag="PK077_06415"
     CDS             1936..4089
                     /locus_tag="PK077_06415"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRN23971.1"
                     /translation="MTPDSVHLLTRSSQMTLCKDEQGRVRRAYYGPRLHQPEDALENA
                     ADAPLLYSSLADSVTGLPNASGEYCICVTQTDGALSLSLECQGWEVLELDDDREEAVF
                     YGKDPSYPVRVEIHVRSHRESDTFLQWAVVRNEGKEGIRLHRAASAQLGLRAERYFVT
                     SFRGTWGGESLMSEEEVARGHELALVSGTGTRTAQEGSPGFIISLDGPAREDSGEVVL
                     GALAWSGNYRIWFRHSPYHYLFAGAGLDTAPAPYLLDGGGVFETPPLILTHSKNGKGE
                     ASRRIHRWARRYGLRGGGTERPTLLNSWEGVYFTFTEKVLHGMMKRAADLGIELFVLD
                     DGWFGNRFPRNDARAGLGDWQVNRAKLPHGLEGLIRHAEELGIRFGIWVEPEMVNPHS
                     ELYQNHPEWVIGLPHRENRQERSQYLLDLSNPHVYRYILDAMRKLLGEHPGISYVKWD
                     CNRKISDPGSPWLDACRQGNLPIDYVRGYEHVLDTLVSEFPDVTFQACSSGGGRADYG
                     TMRRHHEFWTSDNTDAYERVFMQWGIGHLFPAISMAAHVTASPNHQTGRSAPLKFRFD
                     VAMSGRLGFELQPCDMTEEDMAFSKRALSEYKRIRPVVQFGDLYRLSSPYESRVASLM
                     YVCGKRAVVFAWLMDKWLADSPPPLRLKGLNPAARYLVREINMNEDGSLTNVHEQVLG
                     GDFLMDAGIRIRWKKAFQSVCLEVAETNPDAPASS"
     gene            complement(4246..4689)
                     /locus_tag="PK077_06420"
     CDS             complement(4246..4689)
                     /locus_tag="PK077_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23972.1"
                     /translation="MNHPLKFIATTGIVLFFLMLKTGSLAAAREEQPRQPDAQQQEEE
                     KDTPVITNPAVFNEKDLDKLLEKQREYIKKHYPDYRIRGKGEEHDVMDGRHYSEFYLQ
                     NEDEEWVLTIRFDIEEAYQAYMKQHQKEIEREAARLIREGLIKIE"
     gene            complement(4788..5219)
                     /locus_tag="PK077_06425"
     CDS             complement(4788..5219)
                     /locus_tag="PK077_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23973.1"
                     /translation="MKSISNIFAIFFSCMLGLISQIEAQEEQPRQLNEQQQEEEKDIP
                     VIMNPTIFDEKDFDKLLEKQREYVKKHYPDYQIWAEGEYTDYSDGHRYSFFNLQNEDE
                     EWVLTIRFDIEEAYQAYMKQHQKEIEREAARLIREGLIKIE"
     gene            complement(5233..5721)
                     /locus_tag="PK077_06430"
     CDS             complement(5233..5721)
                     /locus_tag="PK077_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23974.1"
                     /translation="MKKKPSIRIWPYIAAMILCSFSFLPASAECKAELGDFISYTTAS
                     GSKPAALGAAEKGLGKISKKVLSEIAVQAIKKASGLLTLAENLKTDKAFRHVHIVYIY
                     RFVHHGKVESEWKIGHMTNISRSGVDYHPPKDIPYVVKEGINKISNLLKKQLKEDCKN
                     HQ"
BASE COUNT         1263 a         1554 c         1687 g         1318 t
ORIGIN      
        1 cattcatcct gacggcgcgc ctggggcgca tagaagagct gcttccactc ccggctgacg
       61 aaggaagaag gctctgtttc cgaagccgga ggcgccagaa acgcctggaa ggcgctggaa
      121 gaacagcaat aatagggaat gttgaaccgg ggcgccattc cgaacgccag cagaagccat
      181 atccagacgc ggaaaccggg acgccgcgtc caggaggaag ccagaaaggt aaggaggcag
      241 gccatggccg gaacgcttcc tttgcaaagc aggtcattga cgtctccgtt cacgtaaaaa
      301 aaaggcagaa tcaggccggt tgccagagta cagataaaga ggggattcct ctggaaatcc
      361 cgccagcaga acagggcgta cagcaggaat gaggtcgcca taagcaggaa atcccccgga
      421 cgcgtataaa aaggccgcat gcccatgtgg gaggatggcg cgtttaccgc cccataataa
      481 agaagaaaac acgttcccag cagaacggcg gccgaaaagg agggatggag caggacatcc
      541 cggagaggga ggacgccttt ccattggcgg cgccactggc aggccgccag gggcagccct
      601 ccaagggccg cgagggggga ataaaaggcc gtggctgcga acagaatagg catccagtat
      661 aggggcatgc gtcctgaagc caggaagatg aaaaagagca gaacaggaat ggatgcgttt
      721 gcgctgcaca tgatttgttc cgccgtgccg aagtaatgca ggcggaacag gttcagcccc
      781 agccagcact ggagattcag cagagagccg aatgccgcca tcagcagggc gagcagcagg
      841 gagcggcgtc cggagaaaac gcagagccac agccaggaaa ggaacaggcc ccaggctccc
      901 cacagaacga caagcatgcg cgcggcgtcc agccccgcca gttttcccgc cagggctggg
      961 accagccaga accccgtgtt gtaaatcagc acgttcccgt ccggaaagat gacgggccac
     1021 gggctggcga ccaattcacg cagacaggca tttctgacga tatggtcagg gtgctggttg
     1081 acccactccc cccatccgga aagcgccatc agggcggcaa acgccgccag ggcgagaaat
     1141 gaagagcggg tgaacagggg gaaggcgggg agagaatccc ttgtttgcgg ttccttgcgt
     1201 tttcggggcg ccagtcccgc agccgtcagg agcgtgagag ggagagcggc gtaccagtgc
     1261 atccacccgg ccaggaatac cagcacgggc agcagaagcc aggcgcaggt gtgccacttc
     1321 agcgcggttg cgttgaacgc ttgttccggg cacggggcat cagcggcagg gggaggcatt
     1381 tccccggaac cttaatgggg ccggcctgcg ggggcaagga ggaaactcgc agaccggaac
     1441 ggaagggggc atttgtatgg gtatggtggc aggaatgagc gcgcgccgtt gcctctttac
     1501 cggaaaaacg gggcaggtca tgctgggagg cctatgaata aaacacaatt tatcagggag
     1561 ctccagcgtg agctggggaa ggacgttact tcccggcagg cggaacacgc gttgaacgcg
     1621 gtgctggaga cgattgcgcg gtcggtccgg aggcggtcgc tggtgaaatt ccggggcttt
     1681 ggcactttcc gcgtcaagcg ccgccgcgcc cgcatcgtac gccatccgga aaacggcggc
     1741 aggctgctgg tgcatgaatc gaaaaccatg aaattccgcc cctcttccct tctgtggaag
     1801 gagtagggaa gccgttcggc agctcctgtc attttgccat tgacttcccc gtccgcggag
     1861 gcccttaatg tggataaggc aggaacattt ttgcctgcct gcataagtaa ctggcccctt
     1921 ccacgtttgc cgatcatgac tccggattcc gttcatctgc tgacccgttc ctcccagatg
     1981 accctgtgca aagatgaaca gggccgtgtg cgccgcgcct attacggccc gaggctgcat
     2041 cagccggagg atgccctgga aaacgccgcg gatgctcctc tcctgtattc ctccctggcg
     2101 gattccgtaa cagggcttcc caatgcgtcc ggcgagtact gcatttgcgt gacccagacg
     2161 gacggggccc tttccctgag tctggaatgc cagggttggg aagtgctgga actggatgac
     2221 gaccgggaag aagccgtctt ctatgggaag gacccttcct accctgtgcg ggtggaaatc
     2281 cacgtgcgct ctcacaggga atcggacacc tttttgcagt gggcggttgt tcggaacgaa
     2341 gggaaagaag gcatccgcct gcaccgcgct gcgtccgcac aactgggttt gcgcgcagaa
     2401 cggtacttcg tcaccagctt ccgcggcacc tggggcggcg agtccctgat gagtgaggaa
     2461 gaagtggccc gggggcatga acttgccctg gtatccggaa ccggcacccg caccgcccag
     2521 gagggaagcc ccggattcat catctctctg gacggtccgg cgcgggagga ttccggggag
     2581 gttgttctgg gggcgctggc atggtccggc aattaccgga tatggttccg gcacagcccg
     2641 tatcattacc tttttgccgg agcgggactg gatacggctc ccgcgcccta tctgctggac
     2701 ggcggcggcg tgtttgaaac gccgcccctc attctgacgc acagtaaaaa cggcaaggga
     2761 gaggcctccc ggcgcatcca ccgctgggcg cgccgctacg gcctgcgcgg cggaggaaca
     2821 gagaggccaa ccttgctgaa ttcctgggag ggggtgtatt ttacctttac ggaaaaagtg
     2881 ctgcacggca tgatgaaacg tgcggcggac ttggggatag agcttttcgt gctggatgac
     2941 ggctggttcg gcaaccggtt cccccgcaat gacgcccgcg cgggattggg ggactggcag
     3001 gtgaaccgcg ctaaattgcc ccatgggctg gaaggcctta tccgccatgc ggaagaactg
     3061 gggatacgct tcggcatctg ggtggagccg gaaatggtga accctcattc ggagctttat
     3121 cagaaccatc cggagtgggt catcggtctc ccgcatcggg aaaacaggca ggaacgcagc
     3181 cagtatttgc tggacctgag caatcctcat gtatacaggt acattcttga cgccatgcgg
     3241 aagctgcttg gagaacatcc gggcatttcc tacgtcaaat gggattgcaa ccgcaaaatt
     3301 tccgaccccg gctctccctg gctggacgcc tgccgccagg ggaatctgcc cattgactat
     3361 gtgcggggct atgaacacgt tcttgatacg ctggtatcgg aattcccgga tgtgacattc
     3421 caggcctgtt cttccggcgg aggccgcgcc gactatggaa ccatgcggag gcatcatgaa
     3481 ttctggacgt ccgacaatac ggacgcttat gaacgcgtgt tcatgcagtg gggcataggc
     3541 cacctgttcc cggctatttc catggccgcc catgtgacag cctcccccaa ccaccagacg
     3601 ggccgctccg ctccgctcaa attccggttt gacgtggcca tgtccggccg gcttggtttt
     3661 gaacttcagc catgcgacat gacggaggag gatatggcct tttccaaacg tgctctgtcg
     3721 gaatacaagc gcatccgccc cgtagtccag ttcggtgact tgtaccggct ttcctccccg
     3781 tatgaaagcc gggtggcctc cctgatgtat gtatgcggaa aacgggctgt cgttttcgcc
     3841 tggctgatgg ataaatggct ggcggattcc cctccgcccc tgcgcctgaa aggcctgaat
     3901 cctgccgcca ggtacctggt gcgggaaatc aacatgaatg aagacggctc tctcacgaac
     3961 gttcacgaac aggtgctggg cggcgatttc ctgatggacg ccggcatccg catccgctgg
     4021 aaaaaggcct tccagtccgt ctgcctggag gtggcggaaa caaaccctga tgctccggct
     4081 tcatcatgaa atcggcgttt ggcgtgggag ccatagccgc cttactgatg tgatgagtta
     4141 ggagttctct tagaggtgag ggtatacagc caatgcggca gaagacaggc gcattccaat
     4201 gccggtttgc cgatattcct ctctttttct acaggaaaag gccgatcatt caatcttgat
     4261 gaggccctcc ctgatgagcc gggccgcttc ccgttctatt tccttttgat gttgtttcat
     4321 gtaggcctgg tacgcttcct caatatcgaa acggatggtc aggacccatt cctcatcttc
     4381 gttttgcaaa taaaactctg aatagtggcg gccatccatg acatcatgtt cctcgccctt
     4441 ccctctgatg cggtaatctg gataatgttt ttttatatat tcccgctgtt tttccaggag
     4501 cttgtccaaa tccttctcat taaacacagc ggggttggtg atgacagggg tatctttttc
     4561 ttcttcctgc tgctgtgcgt ccggctgccg gggctgttct tcccgcgctg ccgcgagact
     4621 tcctgttttc agcatgagga aaaacagaac aatgcccgtg gttgcgatga atttcagagg
     4681 atgattcata tggaattctg ttatggtttt ttccttggga aaacaagaaa ggtcatgaac
     4741 gttccaatgc tggttcgtgt ttcctcttcg ttacaggaaa aggccgatca ttcaatcttg
     4801 atgaggccct ccctgatgag ccgggccgct tcccgttcta tttccttttg atgttgtttc
     4861 atgtaggcct ggtacgcttc ctcaatatcg aaacggatgg tcaggaccca ttcctcatct
     4921 tcgttttgca aattaaaaaa tgaatatcga tgaccatcag aataatctgt atactctcct
     4981 tcagcccaga tttgataatc tgggtaatgt tttttcacat attcccgctg tttttccaga
     5041 agcttatcaa aatctttctc gtcaaaaatg gtaggattca taatgacagg aatatctttt
     5101 tcctcttcct gctgctgttc gttcagctgc cggggctgtt cttcctgtgc ctcaatctgg
     5161 ctgatgagcc ccaacatgca ggagaaaaat atggcgaata tgtttgatat ggatttcata
     5221 tttctgttca ggttattggt ggtttttgca atcttccttt aattgttttt ttaagagatt
     5281 actaatttta tttattcctt cctttactac ataaggaata tcttttggag gatggtagtc
     5341 cactccggac cgggatatat tggtcatgtg gcctattttc cattctgatt ctacctttcc
     5401 gtgatggaca aaccgataga tataaacgat atgcacgtgg cggaaagcct tatccgtttt
     5461 cagattttca gccagggtaa ggaggccgct cgcttttttg atggcctgga cggctatttc
     5521 gctcaatact tttttggaga tttttcccaa tcctttttct gccgcgccca acgcagccgg
     5581 tttggaaccg ctggcggtag tataggagat aaaatcacct agttcggcct tgcactctgc
     5641 tgaagcgggc aggaagctga aagagcaaag gatcattgcc gcgatatatg gccatattcg
     5701 tatggatggt ttcttcttca tatcaattta tttccgtgga gccgggtttc attcccgcgt
     5761 tcttttccgt tgaggcggaa attttttcaa taaactcttt tccctctctg tttaacttcc
     5821 ca
//
LOCUS       DAOMSU010000125         5813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1184_length_5813_cov_2.453456, whole genome shotgun sequence.
ACCESSION   DAOMSU010000125 DAOMSU010000000
VERSION     DAOMSU010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5813
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1184_length_5813_cov_2.453456"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK077_06435"
     CDS             <1..332
                     /locus_tag="PK077_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23975.1"
                     /translation="EKMAGAQQDSMVGAVEEKVLPSPTADDRIACRPEEEEEEPPPAP
                     NVRIRKGTLLTHREYGTGIVVKLDESSGRMTVKFGEEKKLFPYPRSLEQGKLIPAEKA
                     IPEKEGN"
     gene            complement(363..1712)
                     /locus_tag="PK077_06440"
     CDS             complement(363..1712)
                     /locus_tag="PK077_06440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRN23976.1"
                     /translation="MKKLIPLALPVLVVNLSIVGMGAVDAIVAGRAGVTDMAAVALGS
                     SVYLPVALFACGVLMIIGPVIANMRGKSHESRVGYMTNHGLWLALMLSLVSMPVIYVL
                     RNVFGWISDDAAMCRMASAYMFAIMWGLPANLGFVALKSLNEGSNMTRPAMYVGLCGL
                     LLNIPLNYMFVFGMYGFPRMGGAGCGAATAVIFYIEFLLMFLLVYFNPKHRPYRRHII
                     SWRRPTPSVITHLVRLGVPIGVSQLCEVMLFCAAALVLAPLGETQVASHQIAGNVGGL
                     VFMLPLSVGLAASIRVAYHHGRNDLAGTRSAILSSYVLVLTICLCTFGGITLFREQIV
                     HLYNDSELIVSTASVLLVLAAAYQLPDCLQVLSVGVLRGFRDTASITFITFFSYWMVG
                     FPACYILARTDWIVPAMGARGIWTGFIIGLSVAAVLLLWRVVRTVKREFKMMRLHEQ"
     gene            complement(1882..2958)
                     /locus_tag="PK077_06445"
     CDS             complement(1882..2958)
                     /locus_tag="PK077_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HRN23977.1"
                     /translation="MTTQQELTPELIRAALTTVKFPGFSRDIVSFGLVKKIDIDAENN
                     VTIDLVIESKNADIPRYIFEGVHGVMKHLPGVKHCDVNIEHKAPEAKKGINDDPSTWK
                     SSVPGAKHVIAVASGKGGVGKSTVSANLAVALSKLGYSVGLVDLDIYGPSMSLMFGTK
                     ERPGANENDEFIPVTAHGVKLLSMGLLINESDPVAVRGPLATRYVQQFLRNVAWGDVD
                     FLILDLPPGTGDIQLTIVQTAELDGVVVVTTPQEVALIDARKAIGLFERVETPILGII
                     ENMSYFQCPSDGKIYHIFGEGGGEREAAKLGVPLLGKIPLDISTRSGGDEGRPVALED
                     PGQNPVSAAFRQVAEQCARVVLDR"
     gene            complement(3212..3424)
                     /locus_tag="PK077_06450"
     CDS             complement(3212..3424)
                     /locus_tag="PK077_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRN23978.1"
                     /translation="MEDDIICTEKIIAERKTFFLDLKQNARGKVVRITEKVSSNRDRI
                     MVPAEILDDFIAALQDIRETLKQQGE"
     gene            complement(3858..>4869)
                     /locus_tag="PK077_06455"
     CDS             complement(3858..>4869)
                     /locus_tag="PK077_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HRN23979.1"
                     /translation="HNDCELAVANSLAAVNSGAIQVQGTVNGIGERCGNANLCSVIPN
                     LQVKMKGFSCLSGASLTRLKSTAAFVSEVSNLAPFRRQPFVGNAAFAHKGGVHVSAIM
                     KEAALYEHIDPSLVGNAQRVLMTEQGGRSNILSLSRTLGFELEKGDPLLDVLSAAVKK
                     NAALGYDYVAAPASAELLFLRHMPDNALKPYFNILRTVVLTSRHEMDPDMMVEASLKL
                     DVHGNVEHTAAGGFGPVHALDRALRRALTRWYPELEQMHLIDYKVRVLSPTRTNIPEA
                     EDENGTGSNVRVLIESSDGVATWTTVGVSYNIIEASLEALADAVTYKLYKTEQARWRA
                     EC"
     assembly_gap    4870..4879
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4880..5573)
                     /locus_tag="PK077_06460"
     CDS             complement(<4880..5573)
                     /locus_tag="PK077_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HRN23980.1"
                     /translation="MSTKIFLYDTTLRDGAQSEDVNLSATDKVRIARQLDYLGMDYIE
                     GGWPGANPVETEFFNAMRGVGLRNAKLAAFGSTHHPSHTPETDPTLTALISSGARVAA
                     VFGKSCPRHVEVALGISRERNLEIIGNSISFLKKNMEEAFFDAEHFFDGFKREPEYAL
                     AVLRTAWEHGADCLVLCDTNGGTMPEEIGSIIRTVRERLPHALLGIHAHNDIGCAVAS
                     SVLAVENGAVQVQ"
BASE COUNT         1312 a         1734 c         1617 g         1140 t
ORIGIN      
        1 atgaaaagat ggccggagcc cagcaggatt ccatggtcgg cgctgtagag gaaaaagtcc
       61 ttccctcccc aacggcggat gaccgcatcg cctgccgtcc ggaagaggaa gaagaggaac
      121 ctccgcccgc tccgaacgtg cgtattcgta aagggacgct ccttacccac cgggaatacg
      181 gcacgggcat tgtagtaaag ttggacgaat cctccggccg aatgaccgta aaatttggcg
      241 aagagaaaaa actcttcccc tacccgcgaa gcctggaaca aggaaaactc atccctgctg
      301 agaaggcaat cccggaaaaa gaggggaatt aacctctcca taaaaacagg agggggcgtc
      361 ccttactgtt catggagccg catcatctta aactcccgtt taacggtacg gacgacgcgc
      421 cagagcagca gcacggccgc tacggacagg ccgatgatga atcccgtcca gatgccgcgc
      481 gctcccatgg ccgggacaat ccagtccgta cgggcgagga tgtagcacgc cggaaatcct
      541 accatccaat aagagaagaa cgtaatgaag gtaatggacg ccgtgtcccg gaatcctctc
      601 agaaccccga cggaaagcac ctgcaaacag tccggaagct ggtaggccgc cgcgagaacc
      661 agcaggacgg aagccgtgct gacaatcagc tccgagtcat tgtacaggtg cacaatctgc
      721 tcgcggaaca gggtgatgcc tccaaaggtg cacagacaga tggtgagcac cagcacatag
      781 gaagacagaa tggcggatct ggtgcctgcc aggtcattcc tgccgtgatg gtacgccacg
      841 cggatggaag ccgccagccc tacggagagc gggagcatga acaccaggcc tcccacgttc
      901 ccggcaatct ggtggctcgc cacctgcgtc tctcccagcg gagccagcac cagagccgcc
      961 gcgcagaaga gcatcacctc gcacagctgg gaaacgccta tcggcacgcc caaacgaacc
     1021 aggtgcgtga tgacggaagg cgtaggccgc cgccaggaaa tgatgtgcct gcggtacggc
     1081 ctgtgcttgg gattgaagta aaccagcagg aacatcagca ggaattcaat gtagaaaatg
     1141 actgccgtgg ccgcgccgca ccccgctccg cccatgcggg gaaaaccgta catgccgaag
     1201 acgaacatgt agttcagggg aatgttgagc agcaggccgc acaatcccac gtacatggcg
     1261 ggacgcgtca tgttggagcc ttcgttcagg ctcttcaggg ccacgaaccc caggttggcg
     1321 ggaaggcccc acataatggc gaacatgtag gcggaagcca tccggcacat ggcggcgtca
     1381 tcggaaatcc agccgaatac atttctcaac acataaatga ccggcatgga aaccaggctg
     1441 agcatcaacg ccagccacag gccgtggttg gtcatgtagc ccacgcggct ttcatggctt
     1501 ttcccccgca tgttggcaat cacggggccg atgatcatca gcacgccgca ggcgaagaga
     1561 gccacgggca ggtacacgga agaccccagg gccacggcgg ccatgtccgt cacgccggcg
     1621 cgcccggcca caatagcgtc caccgccccc atgcccacaa tggagaggtt gaccaccagc
     1681 accggaaggg ccagcggtat caactttttc atttccttca aattccacgt cttattcatt
     1741 ttttatttcc ttccatgcat tcctcattca aaggagccgg aacatgccgg caaacaagcc
     1801 ggaccggagc ggcaggctcc aatccggctt caaatttaag ggttaccaac cgtaccgggc
     1861 cttccgcccc gtccgggaca ttcagcggtc aagcaccaca cgggcacatt gttcagccac
     1921 ctggcggaac gcggcggaaa ccggattttg cccgggatct tccagggcca cggggcggcc
     1981 ttcgtccccg ccggagcggg tggaaatatc cagcggaatc ttgccgagca ggggcactcc
     2041 cagtttggcc gcttcccgtt cgcctcctcc ttcgccaaag atatggtaaa tcttgccgtc
     2101 agaggggcac tggaaataac tcatgttttc aattatgccc agaatggggg tttccacccg
     2161 ttcaaagagg ccgatcgcct tgcgggcgtc aatcagcgcc acttcctgcg gggtggtcac
     2221 taccaccacg ccgtccagtt cggccgtctg gacaatggtc agctgaatgt ctccggtgcc
     2281 gggaggcagg tccagaatca gaaaatccac atccccccat gccacgttgc gcaggaactg
     2341 ctggacgtag cgtgtcgcca gaggaccgcg caccgctaca ggatcggatt cattaatcag
     2401 cagccccatg gaaagaagtt tcacgccatg ggccgttacc gggataaatt cgtcattctc
     2461 attggctccg gggcgttcct tggtgccgaa catcagggac atggaagggc cgtaaatatc
     2521 caggtccacc agaccgacgg aataccccag cttgctcagg gccaccgcca ggttcgcgga
     2581 aacggtggat ttgcccaccc cgcccttgcc ggaagccacg gcaatgacat gcttggcgcc
     2641 gggaacggag gatttccagg tagagggatc gtcattgatt ccttttttgg cttccggagc
     2701 cttatgctcg atattgacgt cgcagtgctt cacgccgggc aaatgcttca tcacgccgtg
     2761 aacgccttca aaaatgtaac ggggaatatc cgcatttttg ctctcaatca ccaaatcaat
     2821 cgtgacgttg ttttcagcgt caatgtcaat ctttttcacc agcccgaaag atacgatgtc
     2881 gcggctgaac cccggaaatt ttaccgtggt cagcgcagcc cgtatcaatt ccggagtcaa
     2941 ttcctgttgt gtcgtcatat ctctgaaata ttcatggttg catcaacatt ccggccccgc
     3001 gcctccggaa tgcagaatgc tccggaatat gaaaaaaggg gacggcaaaa ccgggaagta
     3061 aaaatactcc cttttttatt tttgacaacg cctaattcag acaaaacggc tttttgcccg
     3121 ccggatttgc aatgccccgg atactaagaa atggcgggac ggccgcagcc ccgctattgg
     3181 tgaaataaaa aacaggaggg agggggaaaa tttattcccc ctgctgcttc agggtctccc
     3241 ggatatcctg gagagcggcg atgaaatcat ccaaaatttc cgcaggcacc ataatccggt
     3301 cccggttgga gctcaccttt tccgtaatac gcaccacttt gccgcgcgca ttctgtttga
     3361 gatcaagaaa gaacgtcttg cgttccgcaa tgattttttc tgtacaaata atatcgtcct
     3421 ccacggctgt aatctacctc tggttgaatg aggatttcag gataaagctt ccccgctggc
     3481 gggtcaatgt tttatttacc ccggcagatg cggccgggaa gcaaaaaccg gaaaaataac
     3541 ggtattttcc cggcagggca tccgttctcc gggcaaactg gcagcaatcc ggcgtcccgg
     3601 cgttaactgc ggcaacgtga ttgaccaaca tgaatggaga acaggaaaga acacaggagc
     3661 cgtctcaaaa aagaacactg tcgcccgggc ctgttcacga agctcattgc tcctcttcct
     3721 gttcattttg agccattcaa ccggcaggag gaaccttttc cgcccttcag cttccggccc
     3781 gccccacgca agggaacggg aagaaaatgg aaaagccatg agaaccgccc acgtcccccg
     3841 gaaaggagcg ccgcccatca gcattccgca cgccatctgg cctgctccgt cttgtagagc
     3901 ttgtacgtga cggcgtccgc cagggcttcc aggctggcct caataatgtt gtaggaaacg
     3961 cccacagtgg tccaggtggc gacgccgtcc gaggactcaa taagcacgcg gacattggag
     4021 ccggttccgt tttcatcctc cgcctctgga atgttcgtcc gggtggggga aagcacgcgc
     4081 accttgtagt cgatgaggtg catctgttcc aattccggat accagcgcgt cagggcgcgg
     4141 cgcagagccc tgtccagcgc atgcacgggg ccaaagcccc cggcggcggt gtgctccaca
     4201 ttgccgtgga catccagctt gagggaggct tccaccatca tgtccgggtc catttcatgg
     4261 cgtgaggtca gcaccacagt gcgcaggatg ttgaaatacg gtttcaaggc attgtccggc
     4321 atgtgccgca ggaagagcag ctccgcgctg gccggggcgg ccacgtaatc ataccccagc
     4381 gcggcatttt tcttcacggc ggcggaaagc acgtccagaa gagggtctcc cttttccagt
     4441 tcaaaaccca gggtgcggga cagggaaagg atgttgctcc tgccgccctg ctccgtcatc
     4501 agcacgcgct gggcgttccc caccagggag gggtcgatgt gctcgtacaa agcggcttcc
     4561 ttcataatcg cgctgacatg cactccgccc ttgtgggcga aagcggcgtt ccctacgaag
     4621 ggctgccgcc ggaaaggcgc cagattggat acttccgaga caaaggcggc agtggatttg
     4681 agccgcgtca gggaggcgcc gctgaggcag gaaaagcctt tcatcttcac ctggaggttg
     4741 gggatgacgg aacaaagatt ggcatttccg caacgctccc cgatgccgtt cacggtcccc
     4801 tgcacctgga tggctccgct gtttaccgcg gccaggctgt tggcgacggc cagttcacag
     4861 tcattgtggn nnnnnnnnnc ctgcacctgg accgcgccgt tttctaccgc cagcacagag
     4921 gaggccaccg cgcagccgat gtcattgtgg gcgtggatgc ccagaagggc atgcggcagg
     4981 cgttccctga ccgtcctgat aatggagccg atttcctccg gcatggtgcc tccgttggta
     5041 tcgcacagca ccaggcagtc cgctccatgt tcccaggctg tccggagcac agccagggcg
     5101 tattccggct cccgcttgaa gccgtcaaaa aagtgttctg cgtcaaaaaa ggcttcttcc
     5161 atatttttct taaggaagga aatggaattg ccgatgattt ccagattgcg ttcccgggaa
     5221 atgcccaggg ccacttccac atggcggggg caggatttcc caaaaaccgc ggccacccgc
     5281 gcgccgctgg atatcagggc ggtcagcgtg gggtccgtct ccggagtatg ggaaggatgg
     5341 tgcgtgcttc caaaggcggc cagctttgca ttccttagtc cgacacctct catggcgttg
     5401 aaaaattccg tttctaccgg attggcgccg ggccagccgc cctcaatgta atccatgccc
     5461 agataatcca gctggcgggc gatgcggacc ttgtccgtcg cgctcaaatt gacgtcttca
     5521 ctctgcgcgc cgtcccgcaa cgtagtatca taaagaaaga ttttggtgga catccccttg
     5581 attcatacat gcaaacggga agccgttcaa gaccgggccc cggcttttcc cctgatgcag
     5641 acacgaaaag cgcaaggctt gaaggagaaa cctgcttccg gtaggatggc cgccatgagt
     5701 ttactcaggc agtctcttca tctctttttc ctcttcttct ccgtggccct tccctccccg
     5761 gcggccactt ccgccccccc tttcctggac agggagcgtc ccatccgctg gag
//
LOCUS       DAOMSU010000126         5807 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1186_length_5807_cov_3.301982, whole genome shotgun sequence.
ACCESSION   DAOMSU010000126 DAOMSU010000000
VERSION     DAOMSU010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1186_length_5807_cov_3.301982"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..1740)
                     /locus_tag="PK077_06465"
     CDS             complement(217..1740)
                     /locus_tag="PK077_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRN23981.1"
                     /translation="MSLHIESTENALAELKKQRTKTLTAAISVSFLGIALMGLLLYLV
                     HIIVAVPEDPPMVAYATQEGDNPDIDTPEVTQTTQRPSSSSTSAQVKVIAAVATADVA
                     VVNPDIEVDVPSEELSMGIDIGDGFGTGTGDGDGGGIPGIISKRCDLNDRMKRITENG
                     GTPQCEEAVVKSLRWLKKQQNKDGSWGGAPHNYKCAMTGMALLSYLAHCETPLSEEFG
                     ETVLKGISFLVDQGMKNPVLSLVPQRNEVSYDHAVATYALCEAYTFCKQMDIPSISNL
                     EKVVIKAVDKIVDNQNTDGGWAYNYNTKSGAHTDLSVTGWNVQALKAAEHGGIRPSKG
                     EIRTALRKAAAYCQKCGKPDGLFTYMQEGREDAAARPSLVGVGVLSLQMCGNGSDSAA
                     RKGLDWMLKNTNQPFNWKANNTSSNLYQHYYGVQAAMNRGGDVWKAYNKAFRDSTLGA
                     QATDGSFTPNGFPGPGGLVNSNGGSINDKIYRQCLATLMLEVYYRFLPGTGEGTRNS"
     gene            complement(2142..>3104)
                     /gene="galE"
                     /locus_tag="PK077_06470"
     CDS             complement(2142..>3104)
                     /gene="galE"
                     /locus_tag="PK077_06470"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRN23982.1"
                     /translation="GYIGSHTVRQLVKTGADVTVLDNMVYGHAGALVDPEVKLVKGDL
                     GDASVVYPLLMKGNFDAVIHFAAFINVGESVQTPLKYYMNNIARPLVLLGAMQAAGVK
                     RFVFSSTCATYGVPTQIPIPETEKQDPINPYGSSKYMLEKVCRDCDRAWGLKSVFLRY
                     FNASGCSEDGLIGEDHEPETHLIPNILLTLTGEKEYIEVFGTDYDTPDGTCIRDYIHV
                     NDLADAHLKAVDYLMKGGATECFNLGTGLGLSVREILETAEKVTGKKIPVRYGPRREG
                     DPPRLIANPKKAKEILGWEAKCKDAGAIVETAWKWMTGPRRGHY"
     assembly_gap    3105..3114
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3466..>3645
                     /locus_tag="PK077_06475"
     CDS             3466..>3645
                     /locus_tag="PK077_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HRN23983.1"
                     /translation="MKPSEIRNVLEDHEVRPSKSLGQNFLTDENVARWIVEQLEIRPQ
                     DCVVEVGPGTGSLTEH"
     assembly_gap    3646..3655
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3656..4852
                     /locus_tag="PK077_06480"
     CDS             <3656..4852
                     /locus_tag="PK077_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRN23984.1"
                     /translation="APLCRKLVLVEFDSRLAEYQKERWAGDPHVEVHHADGASWDPRG
                     LFAEAPVKFLGNLPYSAGGAILQNFLSRPSAVERAVVMLQKEFIDRILATPDDDAFGL
                     LSLRIQKNWIPRALKTIPPEAFHPRPRIDSTVMLLTPRPARELPPYDDRLMDELMRKA
                     FSQRRKQLKKQLPASPPWEGVAASLGLSPSARAEELNLSQWVELARVYDTNPLKDVAQ
                     SGDELFDIVDELNQVTGQGTRREIHEGSLRHRAVHMFLVNKHGAVLLQKRSLWKDRQP
                     GKWDSSAAGHLDAGESYEEAAVRELKEELGVSGCGLQKIADFDAGENNGWEFISLYEG
                     RYSGKVRFPAAEVDSVQWFTPEQIQAWVERRPQDFSPAFIPCWNAWLVANRKNHSNSN
                     TYHDKP"
     gene            4839..5300
                     /locus_tag="PK077_06485"
     CDS             4839..5300
                     /locus_tag="PK077_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23985.1"
                     /translation="MINPDQLSPEQIQSIRTWAEEGLDLNAIQKRLHEDLNIKLTFMD
                     TRFLLQDLDIRISQPEPEPEAEQPGELPGTMPPPASLLGKTQVTVDEITPPHALMAGK
                     VLFKSGAQGAWDIDRTGRINWDATLGEPTADDLREFETELQSVIRSRMGAM"
     gene            5492..5755
                     /locus_tag="PK077_06490"
     CDS             5492..5755
                     /locus_tag="PK077_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23986.1"
                     /translation="MEIPEEMIETLVPAVREQLESPDTAYVKNCLERLTGREKLGEQE
                     ALELMAQALAITINAMVVGGRSFDSGKYKALLKDLPALPDEAP"
BASE COUNT         1220 a         1478 c         1734 g         1355 t
ORIGIN      
        1 gtgtcttggt ccgctgcttc ttcaattccg caagggcatt ttcagtactt tcaatgtgga
       61 gactcataga gggaggtgtc tggttggcat caaggcaaaa actcccgcat ttctgaaaaa
      121 tggggagaat tgccctgtac aggtatgtat tgctggcctg cctgtttaca aggctggcag
      181 cgtactaaag ccagacacgc ctgtctgtca atcagattag gaattcctgg tgccttcccc
      241 cgtgccggga aggaagcggt aatacacctc cagcatcagc gtagcgaggc actggcggta
      301 aattttgtcg ttgatggaac ctccattgct gttcacgaga ccccccggtc ccgggaatcc
      361 gttcggggtg aagcttccat cggtggcctg ggctccgagg gtggaatcac ggaaggcctt
      421 gttgtacgct ttccatacat cccctccgcg gttcatggcg gcctgtaccc cgtaatagtg
      481 ctggtagagg ttggaggaag tattgttcgc cttccagttg aacggctggt tggtgttctt
      541 aagcatccag tccagtccct tgcgggcggc gctgtccgaa ccgttgccgc acatttgaag
      601 ggaaagaacg cctacgccca ccagggaggg acgtgctgcg gcgtcttccc tgccttcctg
      661 catgtaggtg aagaggccgt cgggcttgcc gcatttctgg caataggccg cggctttgcg
      721 cagggccgtg cgtatttcgc ctttggaggg gcggatgccg ccgtgttcgg cggccttgag
      781 ggcctgtacg ttccatccgg tgacggaaag gtccgtatga gcgcccgact tcgtgttgta
      841 attgtaggcc cagccgccgt cggtattttg attgtccaca atcttatcaa cggcctttat
      901 gacgactttt tccagattgg agatggaggg gatgtccatc tgcttgcaga aagtgtaggc
      961 ttcgcacagg gcataggtgg ctaccgcgtg gtcatagctg acttcgttcc tctggggaac
     1021 cagggagaga acgggatttt tcatgccctg atccaccagg aaagagatgc ccttcaggac
     1081 agtttccccg aattcttcgg aaagaggcgt ttcgcagtgg gccaggtagg agaggagagc
     1141 catgcccgtc atggcgcatt tgtaattatg gggagccccg ccccaggaac cgtctttatt
     1201 ctgctgtttt ttcagccagc ggagggattt gactacggct tcctcgcact gcggggtgcc
     1261 cccgttttcc gtgatgcgct tcatgcggtc gttcaaatcg cagcgcttgg agatgatgcc
     1321 gggaatgcct cccccgtctc cgtcgcccgt gccggtgccg aagccgtcgc cgatgtcgat
     1381 gcccatggaa agttcttccg agggaacgtc cacctcaata tccgggttga ccaccgcgac
     1441 gtccgccgtg gcgacggcgg cgatgacctt cacctgcgcg gaggtggagg aactgctggg
     1501 acgctgggtg gtctgggtaa cttccggtgt gtcgatgtcc gggttgtcgc cttcctgggt
     1561 ggcgtaggcg accatggggg gatcttccgg aacggccacg atgatgtgca ccaggtagag
     1621 gaggaggccc atgagggcga tgccaaggaa ggagacggaa atggcggcgg tcagtgtctt
     1681 ggtccgctgc ttcttcaatt ccgcaagggc attttcagta ctttcaatat gcaggctcat
     1741 acagtatgtg aagcttcctg aaaataatag aatgtttttc cggttgaggc gagttcaaaa
     1801 attttgatgg tctatagggt ggtaaatgac cagatatgaa caaaatggaa ttttataatg
     1861 aacggcttat attggttgca tcatataccc gacgggggcg tctgccggga catgggctgt
     1921 gtttctggtt tgccggagag gaatggaaaa ggctttttga agccgggaag gagaagcagc
     1981 acgccgggaa ccgtttgcct ttgaatgttt ttagctgtgg ccggcagagg aagaaagagc
     2041 ggttccggga gaggagcgta cgaagaaatt tgaaaaaaca agccctgcca tggagggcat
     2101 ggcaggacgg aaaagagggt ggccttttgc gggcagaggc attaatagtg gccgcggcgc
     2161 gggccggtca tccacttcca tgcggtttcc acaatggccc cggcatcctt gcacttggct
     2221 tcccagccga ggatttcctt ggctttcttc ggattggcaa tcaggcgggg cgggtcgcct
     2281 tcgcggcgcg ggccgtagcg gacgggaatt tttttgccgg tgactttttc agccgtttcc
     2341 aggatttccc ggacggaaag tcccaggccg gttccgaggt tgaagcattc tgtggccccg
     2401 cctttcatca ggtagtccac cgctttcaag tgggcgtccg ccaggtcgtt gacgtggatg
     2461 tagtcgcgga tgcaggtgcc gtccggcgtg tcgtaatccg ttccgaagac ttcgatgtat
     2521 tccttttccc cggtgagcgt gagcaggatg ttggggataa ggtgggtttc cggttcgtgg
     2581 tcttcaccga tcaagccgtc ctcgctgcag cctgaggcat taaaatagcg cagaaaaacg
     2641 cttttgagac cccaggcgcg gtcgcagtcc ctgcaaactt tttccagcat gtacttggaa
     2701 ctcccgtaag gattgatagg atcctgcttt tccgtttccg ggatagggat ctgggtggga
     2761 acgccatacg tggcgcaggt ggaggagaaa acaaagcgct tgacgccggc ggcttgcatg
     2821 gcgcccagaa ggacgagggg cctggcgatg ttgttcatgt agtacttgag gggggtctgg
     2881 acggattccc ccacattgat gaaggcggca aagtgaatca cggcgtcaaa gttgcccttc
     2941 atcaggagag gatataccac ggaggcatct cccaaatccc ctttgaccag tttcacttcc
     3001 gggtccacga gggctcccgc gtggccgtac accatgttgt ccagcacggt cacgtctgcg
     3061 ccggttttga cgagctggcg taccgtgtgg gaaccaatat atccnnnnnn nnnnagtatt
     3121 taacatatgg aaaataaagt tgcttccgat aaatccggct ccgccggtta caagtacttt
     3181 catgatttta tcaaatttgc tgttgagccg gtttaacttt ttctccggca aaggcgtccg
     3241 gacattttgg agtctgcgga accttccggt tcactgcagg ctgaatccct gttccagacg
     3301 cctgtaaaag tgacatgttt tccccttttt gtcaatttgc gatatatctt gccgccgtct
     3361 ctccgttctt tgcctgcggc ggttggagct tctccgtttt catgccggaa actgccccgc
     3421 tttttactgg ctcctgacag gcatttttgc gaacatcact tcattatgaa accgtccgag
     3481 atcaggaatg ttctggaaga ccatgaagtg cgccccagca agtccctggg gcagaatttc
     3541 ctgacggatg aaaatgtggc ccgctggatt gtggagcagc tcgaaatccg gccgcaggat
     3601 tgcgtggtgg aggtagggcc tggaaccggc tccctgacgg aacatnnnnn nnnnngcacc
     3661 cctttgccgc aaactggttc tggtggaatt tgattcccgt ctggcggagt accagaaaga
     3721 gcgctgggcc ggtgatcccc atgtggaggt tcaccatgca gacggcgctt cctgggatcc
     3781 gcgggggctg tttgcggagg cccctgtcaa attccttggc aatcttccct attccgcggg
     3841 gggagccatc ctccagaatt tcctgtccag gcccagtgct gtggaacgtg ccgtggtgat
     3901 gctccagaag gagtttatcg atcgcattct ggcgacgccg gatgatgatg ccttcgggct
     3961 gttgtccctg cgcatccaga aaaactggat tccccgtgcc cttaaaacca ttccgccgga
     4021 agcgtttcat ccccgtccgc gtattgattc caccgtgatg ctgcttactc cccgccctgc
     4081 ccgcgagctg cctccatatg acgaccgcct gatggatgaa ctgatgcgca aggctttttc
     4141 ccagcggcgc aaacaattga aaaaacagct ccccgcttct cctccgtggg agggcgtggc
     4201 tgcttccctg ggactttccc cttccgccag ggctgaggaa ttgaatttgt cccagtgggt
     4261 ggaattggcg cgcgtttatg ataccaatcc tctcaaggat gtggcgcaga gcggagatga
     4321 attgtttgac atcgtggacg aactcaatca ggtgacgggg cagggcacgc gccgggaaat
     4381 acatgaaggc agccttcgtc atcgcgccgt acatatgttc ctggtcaata agcatggagc
     4441 tgtgcttctc cagaaaaggt ccctgtggaa agacaggcag ccgggcaaat gggattcctc
     4501 cgcagccggg catctggatg ccggggaaag ttatgaggaa gctgcggtgc gggagctgaa
     4561 ggaagaactg ggggtgtccg gctgcgggct gcagaaaatc gcggattttg acgccggaga
     4621 aaataacggc tgggaattca tctccctcta tgaagggcgg tattccggga aggttcgttt
     4681 tccggcggcg gaggtggaca gcgtgcagtg gttcacgccg gagcaaatcc aggcgtgggt
     4741 ggaacgccgt ccgcaggatt tctcgcctgc gttcattccc tgctggaatg catggctggt
     4801 cgcgaataga aaaaatcatt ctaactcaaa tacatatcat gataaaccct gatcaattat
     4861 ctccggaaca gatccagtcc atccggacct gggcggaaga aggactggat ttaaacgcca
     4921 tccagaaaag gctgcatgag gacttgaaca tcaagctgac gttcatggat acaagattcc
     4981 ttcttcagga tctggatatc cgcatcagcc agccggagcc ggaacccgaa gccgagcagc
     5041 ccggagagct tcccggaaca atgcctcccc ctgcctccct gctgggaaaa acccaggtaa
     5101 ctgtggatga aataacgcct ccccacgccc tgatggccgg caaggtttta ttcaagagcg
     5161 gcgcccaggg cgcatgggat attgaccgta ccggccgtat caactgggac gctacgcttg
     5221 gagaacctac ggcagacgac ctgagggaat ttgaaacgga actgcaatcc gtcattcgca
     5281 gccgcatggg agccatgtga gggaaacgga aaatattccg cttgaaatgg actggaagca
     5341 ggaacttgcc gggtggcggt tatttccaat aaaagccttg gctggaatgg aaaatgccgt
     5401 gtccgcatgc cattcgggag agaatgtctg cccggaaaaa atgcgcgttc atttggttcc
     5461 atagtaaaat aaaagcaaca aaaggactat catggaaata ccggaggaaa tgattgaaac
     5521 gctggtgcct gccgttcggg agcagctgga gtcgccggac accgcttatg tcaaaaactg
     5581 tctggaacgc ctgacggggc gggaaaaact gggagaacag gaagctctgg aactgatggc
     5641 gcaggctctg gccattacta ttaatgcgat ggtggtgggg ggccgctcct ttgattcggg
     5701 aaagtacaag gcgctgttga aagatctccc ggctcttccg gatgaggctc cgtgataaat
     5761 ctcgacaatc tccggaattg attcataatc ctttctttca cttgaaa
//
LOCUS       DAOMSU010000127         5804 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1187_length_5804_cov_2.318316, whole genome shotgun sequence.
ACCESSION   DAOMSU010000127 DAOMSU010000000
VERSION     DAOMSU010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1187_length_5804_cov_2.318316"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /gene="pyrH"
                     /locus_tag="PK077_06495"
     CDS             <1..701
                     /gene="pyrH"
                     /locus_tag="PK077_06495"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRN23987.1"
                     /translation="RRILLKLSGEALRSPGSMDNISPEIVARIAEEIAQAHRAGVQIG
                     LVVGGGNFWRGAKASVRGMDRATADYVGMLATVMNALALQSALERAGVPCVVQSAIEM
                     KNVAESFIRLKARSYLDNNKIVIFAAGTGNPFFSTDTTAALRASEINAEVVMKATSVD
                     GVYDADPKKVSGAKRFDTISYQECLERQLKVMDSTAFTLCMDNKKPIIVFDMHTPGNI
                     TRALLGEPIGTTIS"
     gene            753..1319
                     /gene="frr"
                     /locus_tag="PK077_06500"
     CDS             753..1319
                     /gene="frr"
                     /locus_tag="PK077_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRN23988.1"
                     /translation="MDAETLLLETEESMLKAVDFAKQEFSGVRTGKASPGLVENLDVH
                     VSSYGSVMKLKGLAVISTPEPRLILIQPFDPSTVHDIGRAINESKLNLNPLVEGRSIR
                     LPIPALTEERRKDLVKLVKSQAEEARVRVRGARKAAMDSAKKLKAENIVTEDGQRDLE
                     TQIQKLTDKYVKEIDELVAVKEKDIMTI"
     gene            1345..1665
                     /locus_tag="PK077_06505"
     CDS             1345..1665
                     /locus_tag="PK077_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRN23989.1"
                     /translation="MTDNVTNFLASGIEIKGTIRFQNDMHIDGKIEGEIISDKGKVTI
                     GETAQIKGDITAGDVRIYGNVEGKVTSDRCELKQQARVVGDMKTRSLAMEEGAHLLGR
                     TEIG"
     gene            2401..>2824
                     /locus_tag="PK077_06510"
     CDS             2401..>2824
                     /locus_tag="PK077_06510"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HRN23990.1"
                     /translation="MSKESVNIHVNNYCQLRDKQYAVYTEYARKHGLTTKELFILDII
                     YFAQSGCTQAGICERLSATKQTVSAAIKKFWKLGYLSFEESKTDRRIRLVRFTAAGKE
                     YVERIIPPAAQAENDAMAELSGEQQAELVRLTDLFSEQM"
     assembly_gap    2825..2834
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2869..3783
                     /locus_tag="PK077_06515"
     CDS             2869..3783
                     /locus_tag="PK077_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HRN23991.1"
                     /translation="MIINTGGRTDTVQYYTRWLLKRFEEGYVLSRNPLFQHKVTRYEL
                     TPDQVDCVVFCSKNYAPILPHINSITERFHTYFHYTITAYGKEIEPGVPGIDESMATL
                     AKLSEIVGANRIAWRYDPVLLTSEYTVRRHLETFERMASFLSGHIDRCIFSFVEMYRK
                     LQTNMPELIPLTEKDKEELAAGLGAIAAAYNIPIQTCGTNGDYTRYGISSSGCMTLNI
                     LGQANGVQFRNLKHQGMRQGCHCIESRDIGAYDTCLNGCKYCYANTNRQKARENYKLH
                     DPDSPLLLGHLLPSDTVQQGMQRSFLKP"
     gene            3780..4730
                     /locus_tag="PK077_06520"
     CDS             3780..4730
                     /locus_tag="PK077_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM mobile pair protein B"
                     /protein_id="HRN23992.1"
                     /translation="MNADEIKVREIQVKSILSTSNLPVADYSVNPYLGCPHACKYCYA
                     SFMKRFTGHPEPWGTFLDVKYWPEIRNPGKYAGKQLFIGSVTDPYGPQEEIYKRTRAL
                     LEQLQGSGAEISIATKSDLVLRDLDLIKTFPHARVSWSVNTLDERFQADMDMAVSISR
                     RLAAMKAFHEAGIRTTCFISPIFPGITDIPAIVEQAEDKCNLVWLENLNLRGSYKSVI
                     LEYIRKRYPHLVPLYREIYQKGSRGYWEELDAAIRQLAEKRGLPYLRNDDSMHRPFNE
                     PPVIVNYFYHEQIKRSARKEGRFRIRRLPRRPPAVDVKNA"
     assembly_gap    4931..4940
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4941..5189)
                     /locus_tag="PK077_06525"
     CDS             complement(<4941..5189)
                     /locus_tag="PK077_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HRN23993.1"
                     /translation="MIQDINTSIGTFDVSEEDFLFMKNFVANAVYDDYDLLVQLCDSL
                     ALPTGFCLLEKRFVDVTIRYGAHPFTVDRWKKILQIKAF"
     gene            complement(5244..5471)
                     /locus_tag="PK077_06530"
                     /pseudo
     CDS             complement(5244..5471)
                     /locus_tag="PK077_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
     gene            complement(5458..>5804)
                     /locus_tag="PK077_06535"
     CDS             complement(5458..>5804)
                     /locus_tag="PK077_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN23994.1"
                     /translation="ERLYQEGAGSRPAAFLVRCAPVCRGMITRKIIPSFPDKGKSKKR
                     DDEIKQMMAYARKHDFPGKNSVPERCRPLFLAKENIFASHHLNTGGRGGIVMFILVMV
                     LYSDSHDRDGAG"
BASE COUNT         1501 a         1698 c         1394 g         1191 t
ORIGIN      
        1 atcgaagaat cctcctgaaa ctcagtgggg aagccttgcg cagccccggc agcatggaca
       61 acatttcccc ggaaatcgtc gcccgcattg ctgaagaaat cgcccaggcg caccgcgccg
      121 gcgtccaaat aggcctggtc gtgggcggcg gcaatttctg gcgcggggcg aaagccagcg
      181 tgcgcggcat ggaccgcgcc acggcggatt acgtgggcat gctcgccacc gtaatgaacg
      241 ccctggccct tcaaagcgcg ctggaacgcg cgggggtccc ctgcgtggtg cagtccgcca
      301 ttgaaatgaa aaatgtcgcg gaatccttca tccgcctgaa ggcccgctcg tatctggaca
      361 acaataaaat cgtcatcttc gccgccggca ccggcaaccc gttcttttcc acggacacca
      421 cggctgccct gcgcgccagc gaaatcaatg cggaagtggt catgaaggct acctccgtgg
      481 acggggtgta tgatgcggac cccaaaaaag tctccggagc caagcgattc gacaccatct
      541 cctatcagga atgcctggaa cgccagctca aagtgatgga ctccacggcc ttcaccctgt
      601 gcatggacaa caagaagccc atcatcgtat tcgacatgca tactccgggc aacatcacgc
      661 gcgcccttct cggggaaccg atcggcacca ccatcagctg aaccccgcat tccttttcaa
      721 cccgccaaaa acacttagca cccaccatca tcatggacgc agaaacatta ttattggaaa
      781 cggaggaatc catgctcaag gccgtggact tcgccaaaca ggaattctcc ggcgtacgca
      841 cgggcaaagc ctcccccggc ctcgtggaaa acctggacgt acatgtctcc agctacggct
      901 ccgtcatgaa actgaagggc ctggccgtca tcagcacgcc ggaaccccgt ctcatcctca
      961 tccagccctt tgatccgagc accgttcacg acatcggccg cgccatcaat gaaagcaagc
     1021 tgaacctcaa tcccctggtg gaaggccgtt ccatccgcct gcccattccc gccctcacgg
     1081 aagaacgccg caaagacctt gtgaagcttg tcaaatccca ggcggaagaa gcccgcgtgc
     1141 gcgtgcgcgg cgcccggaag gccgccatgg attccgccaa aaagctcaag gcggaaaaca
     1201 ttgtcacgga agacggccag cgcgacctgg aaacgcagat ccagaagctg acggacaaat
     1261 acgtgaagga aattgacgag ctggtggccg tcaaggaaaa ggatatcatg accatttaac
     1321 cccatcccca accattttac gacaatgaca gacaacgtaa ccaacttcct cgcctccggc
     1381 atcgaaatca agggaaccat ccgtttccag aatgacatgc acatcgacgg caagatcgag
     1441 ggtgaaatca tctccgacaa aggaaaagtc accatcgggg aaaccgccca aatcaagggt
     1501 gacattactg ccggagacgt ccgcatctac ggcaacgtgg aaggcaaggt cacctctgac
     1561 cgctgcgaac tcaagcaaca ggcccgcgtc gtcggagaca tgaaaacgcg cagcctcgcc
     1621 atggaggaag gcgcccacct gctgggccgc acggaaatcg gctaaaaaca caaccgccct
     1681 gttcttccct ggcgtgcctt ttgatacggg ccgcttctcc acaaaagcgc aaaagcgcgc
     1741 cggggtttct cagggggcgg cgaaaatgaa gaacggaaaa aggtaccgcc cttttttcca
     1801 aatcccgttc ccgctccgga agcggaattg ctgggagcgc ccggatcatc tcccgttctt
     1861 caggaagcgg acggatgccg ttttacacaa ataccctacg gcagaaaaac cgcttctttc
     1921 ttctgtccga accatcgccg gataccgcgc aggcgcatcg ccgcctgcgt tttttattga
     1981 gtctccacgc tctctccccg cctccggcga aacatcagtc accggagcag gcggaacttg
     2041 ctctggtgca gaaaagcccc atcgggcaag ctctccaact cccgtaaaca tggaaaaacg
     2101 tttccccggt agccacaggc gcctgtcttc cgaagccatg cggcaacaaa actcttctgc
     2161 cgcgaaacag aagatacggg aataaaaacg gaaaatgtac cggattcttg aaaggtcggc
     2221 atccattgcc gctccagtac tgtttccgga aatttgcctg gtcacgcaaa aagccgtccc
     2281 tatcccccgc ctctttatct ggagctttat atttggggct ttacaaaaaa cgcgcgaata
     2341 gctacattct ttttgtcaaa tatcagacta tcaaatatca tactttatcc atctccgcct
     2401 atgagcaaag agagcgtcaa tatccacgta aacaactact gccagctgcg ggacaaacaa
     2461 tatgccgtct atacggaata cgccagaaaa cacgggttaa ccaccaaaga gctgtttatt
     2521 ctggatatca tctatttcgc tcaaagcggc tgcacgcagg ctgggatttg cgaacgcctc
     2581 tccgccacga agcaaaccgt cagcgccgcc atcaaaaaat tctggaagct ggggtacctt
     2641 tcttttgaag aatccaaaac tgaccggaga atcaggctgg tacgcttcac cgcagccgga
     2701 aaggaatacg tggaacgcat catcccccct gccgcgcagg cggaaaacga cgccatggcg
     2761 gaattgagcg gagaacagca agcggaactg gtccgtttaa ccgacctttt ttcagaacaa
     2821 atgcnnnnnn nnnngtctga aatttcaatc catcaacaaa aacacgctat gattatcaat
     2881 acaggaggac ggacagacac cgtccagtat tacacccgct ggctgctgaa gaggtttgaa
     2941 gaaggctatg tcctttcccg caatcccctg ttccagcata aggtgacccg ttatgaactg
     3001 acgccggacc aggtggactg tgttgtgttc tgctctaaaa attatgcccc cattcttccg
     3061 catatcaaca gcatcaccga gcgcttccat acctacttcc actatacgat tacggcctac
     3121 ggaaaggaaa ttgaaccggg tgttcccggc attgatgaga gcatggccac gctggcaaaa
     3181 ctttcggaaa ttgtcggagc aaaccgcatc gcatggcggt atgacccggt attgctgaca
     3241 agtgaatata ccgtcagaag gcatctggag acctttgaac gcatggcgtc cttcttatcc
     3301 ggccacatcg accgctgcat tttcagcttt gtggaaatgt acaggaagct gcaaaccaac
     3361 atgccggaac taatccccct cacggaaaaa gacaaggagg agctggcggc agggctggga
     3421 gccatcgcgg ccgcatacaa catccctatt caaacctgcg gaaccaatgg agattacacg
     3481 cgttacggca tttcatcctc cggctgcatg accctgaaca ttctgggaca ggccaacggc
     3541 gttcaattcc gcaacctgaa acatcaagga atgcggcagg gctgccattg cattgagagc
     3601 cgggacatag gcgcgtatga cacctgcctt aacggctgca aatactgcta tgcaaatact
     3661 aatcggcaaa aggcccgtga aaattacaaa ctccatgacc cggactctcc gctgctgctg
     3721 gggcatctgc tcccttccga taccgtgcag caggggatgc aaaggagctt tttaaaacca
     3781 tgaatgctga tgaaatcaaa gtaagggaaa ttcaggtaaa gagcatcctg tcaacgtcaa
     3841 atctgcctgt ggccgattac tcggtcaatc cttacttggg ctgcccccac gcctgtaaat
     3901 actgctacgc ctcgttcatg aaacggttca ccgggcatcc ggaaccatgg ggaacctttc
     3961 tggacgtcaa atactggccg gaaatacgca accccgggaa atacgcggga aaacagctgt
     4021 ttatcggttc cgtgaccgac ccttacggac ctcaggaaga aatctacaaa agaacaaggg
     4081 cgctcctgga acagcttcaa ggcagcggcg cggaaatcag catagctaca aaatccgatt
     4141 tggtgctgcg ggacctggat ttgataaaaa catttcccca tgcccgcgtt tcctggtccg
     4201 tcaacacgct tgacgagcgc tttcaggcag atatggacat ggcggttagc atctcgcgga
     4261 ggctggccgc catgaaggcc ttccatgagg caggaatccg caccacctgc ttcatctccc
     4321 ccatttttcc aggcattacg gatattccgg cgattgtgga gcaggctgaa gacaaatgca
     4381 acctggtttg gctggaaaac cttaatctgc ggggcagcta caaatccgta attctggagt
     4441 acatcagaaa acgctatccg cacttggtgc ccctctatcg ggaaatttac cagaaaggca
     4501 gccgcggcta ctgggaagaa ctggacgcag caatacggca gctggcggaa aaacggggtt
     4561 tgccgtacct gcgcaatgac gattccatgc accggccgtt caacgaaccg ccggttattg
     4621 tgaattactt ttaccatgag cagatcaaac gttccgccag aaaagagggg cgcttccgaa
     4681 tccgccgcct cccgcggcgg cctccagccg ttgatgtcaa aaacgcctga taaccctgca
     4741 tggaccccca acagcaacgc agtcggccgg cacagaaatt ccccctcccg ggcgatttac
     4801 cctagcgtat cgggatgtgc cgtttctaaa aaatcttccg catcatcacg cataagcccc
     4861 ctcctgacgg agactgtttt caacgacgcc cggcagcaga tcataaatgg aacagcctgc
     4921 tttctcttca nnnnnnnnnn gaacgccttg atttgcagaa tttttttcca ccggtccact
     4981 gtaaacggat gcgccccata ccggattgtt acatccacaa accgtttttc caaaaggcag
     5041 aagcctgtag gcaaggccaa agaatcacat aactgaacca ggaggtcata gtcgtcatag
     5101 actgcgttcg ccacaaaatt tttcatgaac aggaaatcct cttcactcac gtcaaacgta
     5161 ccgatagaag tattgatatc ctgtatcata aacgcatggg aaatacaaat ctgcgcagcc
     5221 ttgctccagc ctcttgccga acaatagcgg tacccgtcta ttaaatgctt ttcggaactt
     5281 actccggcat atcgtccaat atcatgcaat aaccccagga cataagcttt ttcaccggac
     5341 agccctttac aatgtgacgc tatattttca caggcctgcg ctacatagcg ggaatgttcc
     5401 gcccagcctc ccgggttgga ttcatatgct tccctgattg cctgttccgc agtttttcta
     5461 tccggctcca tctctgtcat ggctatcact atagagcacc attactaaaa taaacatcac
     5521 gattcctcca cgtcccccgg tattcagatg atgtgacgca aaaatatttt cttttgctaa
     5581 aaaaagcggc cggcaccgtt ccggaacgga attttttccg gggaaatcat gttttcgggc
     5641 atatgccatc atttgcttta tttcatcatc ccgtttcttg ctttttccct tgtccgggaa
     5701 agagggaatg attttccgtg taatcattcc ccggcagacg ggcgcacaac ggaccaggaa
     5761 cgcggctggg cgggatccag caccttcctg ataaaggcgt tcat
//
LOCUS       DAOMSU010000128         5775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1199_length_5775_cov_2.713462, whole genome shotgun sequence.
ACCESSION   DAOMSU010000128 DAOMSU010000000
VERSION     DAOMSU010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1199_length_5775_cov_2.713462"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(274..1836)
                     /locus_tag="PK077_06540"
     CDS             complement(274..1836)
                     /locus_tag="PK077_06540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HRN23995.1"
                     /translation="MLTDLFVIVIYFLAIFCIGIYAGRKQNSLTDYALGNRSLPWWAI
                     LASILAAEISAATFLGAPGEGYHTRNFTYAQLCIGTILGRIIVGRLFLKPYYDYKVVS
                     IYEYLEKRFGLLTRRTASMVFLVSRVLASGTRLYFAGILLVIAYQFLTGATADASQIV
                     LLYIAALVAISVATTIYTAIGGLKAVVWTDVLQAVVLGVSMLSALWVLFSHIPGGWSS
                     ISAAMNGGDDWKFFSWGTGEGLDFLQQCARVLSQEYTVWAAFLGATFITMATHGTDQD
                     MVQRMLAAKNSKAGTRAVIVSGLLDFPIVIIFLFTGILLYVFYQYNPANLPADTPQLH
                     VFPYFIIHELPNGIRGLLIAGLLATAMGSLSTALNSLATTATRDWYQGIFKPEATERQ
                     LLRCVRWGTAGFSLLLILVGSITAWYVVHHPEVRIIQIALGIFGYTYGSLLGIFLLGM
                     LTRTRGSDSGNVIAMAAGFLVIALLTGLIPLPSGWQQYIPEIAFPWRVTIGTLVTFIV
                     GFCFRNRRLSVR"
     gene            complement(1903..3510)
                     /locus_tag="PK077_06545"
     CDS             complement(1903..3510)
                     /locus_tag="PK077_06545"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRN23996.1"
                     /translation="MKYIFVTGGVVSSLGKGLAAASIGTLLERCGLKVTLQKFDPYLN
                     VDPGTMSPFQHGEVYVLNDGAETDLDLGHYERFVHCSLSRLNNLTSGQVFESVLRKER
                     RGDYLGKTVQYIPHVTDEIKNRLYEVTEKSDVDIIITEIGGTVGDMEGHIFLEALRQF
                     ALEVGRDNVCFIHVTLLPYIKAAGEMKTKPTQQSVAKLREIGIQPDVIICRTEYDMSE
                     DERRKIAMFCNVEAKNVIAFRDVKNTIYECPLDLSQDKIDRIVTRRLGLDVPAPNLAD
                     WQRYVGRVISPSHSIRIAVVGKYIALQDAYKSIYESFTHAGAENDARVEILRIDAEEI
                     EEKGAEAMIGSVDGILVPGGFGDRGIEGKIQTVEYARTKGIPFLGICLGMQVAVIEYA
                     RHICGMDDANSTEFDQKTTHPVISLQEEQKGIQAMGATMRLGAYKALIRPGTLAHKLY
                     GKDSVTERHRHRYEFNPAYRDELENAGLVISAVNDEHGLVEVVELPEHPFFIACQYHP
                     EFQSAPNRAHPLFSGLVSAALEHKSHS"
     assembly_gap    3662..3680
                     /estimated_length=19
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3681..4287)
                     /gene="kdsB"
                     /locus_tag="PK077_06550"
     CDS             complement(<3681..4287)
                     /gene="kdsB"
                     /locus_tag="PK077_06550"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HRN23997.1"
                     /translation="MAADTSHHIIGLIPSRWGSSRFPGKPLHPIAGKPLVQHVWERVC
                     RCSRLDAIAIATDDQRIFDAAVSFGARAIMTSPDHPSGSDRLAEAVRSFPAATHVVNI
                     QGDEPLIDPALIDRLAEALVSDEALSMATVACPISAREDLDNPNIVKVALARNGDALY
                     FSRSVIPYARHPRVAPPLRHLGIYAYRRDFLENYVRWAPTPL"
     gene            complement(4380..>5775)
                     /locus_tag="PK077_06555"
     CDS             complement(4380..>5775)
                     /locus_tag="PK077_06555"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRN23998.1"
                     /translation="KVSYLWILIPILFLPIPSLRDYAPLLAYGITLPCYLWNAMLRRS
                     SLTWNSAPLLDAVVLVLFMHVGYIFLSHPFALGLDVLEDYYGGKGYILFLQALLAYLC
                     LSSLKTTSHELGKVLQWAVFLTIVFTLILTARSLLSPDSAGADLAASAGSAGTLSENS
                     RNSSFLRISILVMQLLIINYSVWQMIKRPWWGALLLLGTVGILLSGFRSAMAQVLFLF
                     FTISLIYRRWFFCLLAPVLGVLLLLLLSSAGMLHSLPFGIQRTLSAVPFLDVSAQAKA
                     NAEDSINWRFEMWSWALDDREHFIQDKIFGDGFSRDISIVKANVYEEAYNLSKDQSAF
                     AWNGQWHSGPISTIQTLGYIGIFLYLILSVVGMTYAWIVSRIYRNHQYKLGILYVSAV
                     YFTKPIFFFLIFGDSITIAMDIISLAIIKVLYSCARREGLYVSLHVRKEYMPLMIRKT
                     QEKRQAAATASISG"
BASE COUNT         1255 a         1463 c         1810 g         1228 t
ORIGIN      
        1 gcgtttccat tccgtttcac gccgcttttc ctttcccggg aaagcggcgc ttttgcgtcc
       61 ggagagaggg ggaagatatc agggcagaaa ggacgaaaga cgattttccc ctgttccggg
      121 gaaaaggaga tactcccgtt taacaaagat gcctccttag ggatttttat ctcctacttc
      181 cctgtttttc gcttgctcct tcacttcctt tcacaggagg tttgaaggga aactgcggct
      241 tatgaatcag gcggggtgac ggcagtcccc cgttcagcgc acggagaggc ggcgattcct
      301 gaaacagaac ccgacgataa aagtaaccag cgttccgatg gtcacgcgcc acggaaaggc
      361 tatttccggg atatattgtt gccagcctga gggcaggggg atgagccccg tcagtaaagc
      421 gatgacaaga aatcctgccg ccatggcaat gacgtttcct gagtcgctgc ctctggtgcg
      481 ggtcagcatt cccaacagga agatgccaag cagggagcca taggtgtagc cgaaaatgcc
      541 cagggctatt tggatgatgc ggacttccgg gtgatgcacc acataccacg ctgtgatgga
      601 acccacgagg atgagcagca gggagaaacc ggccgttccc cagcgcacgc accggagcag
      661 ctgccgttcc gtggcttccg gtttgaatat cccctggtac cagtccctgg tggcggtagt
      721 cgccagggag tttagagccg tggagaggga gcccatggcc gtcgccagaa gtccggcgat
      781 cagcagcccg cggatgccgt tgggaagttc atggatgatg aagtagggaa acacatgcag
      841 ctgcggcgtg tctgcgggga ggtttgccgg gttgtattgg tagaagacat agagaagaat
      901 gcccgtgaag aggaaaatga tgacgatggg gaagtccagc aagccggata cgatgactgc
      961 acgcgttccc gccttgctgt tttttgccgc cagcatgcgc tgcaccatgt cctggtccgt
     1021 gccgtgcgtg gccatggtga tgaaggttgc tcccaggaag gctgcccaca ccgtgtattc
     1081 ctggctcagc acgcgagcgc attgttggag gaagtccagg ccttctccgg ttccccagga
     1141 gaagaatttc caatcgtctc caccattcat ggcggcggag atggagctcc accctccggg
     1201 gatatgggag aacagtaccc acagggcgga aagcatggag actcccagaa cgacggcctg
     1261 gaggacgtcc gtccagacga cggctttcaa tcctccgatc gcggtgtaaa tggtcgtggc
     1321 gacggagatg gcaaccaggg cggcaatgta gagcaggaca atctggctgg cgtccgccgt
     1381 ggcgcctgtc aggaactggt aggcaatgac cagcagaatg cccgcgaagt agagcctggt
     1441 tccgctggcg agcacccggc tgaccagaaa gaccatggag gccgtccgcc gcgtcaggag
     1501 cccgaatctt ttttccaggt attcatagat ggaaacgacc ttgtagtcat agtagggctt
     1561 gagaaagagc ctgccgacga tgatgcggcc caggatggtg ccgatgcaga gctgggcgta
     1621 cgtgaagttg cgggtatggt atccttcccc gggagctccc aggaaggtgg ccgcgctgat
     1681 ttccgccgcc aggatagagg ccagaatagc ccaccagggc agggaacggt tgcccagggc
     1741 atagtctgtc agggagtttt gctttcgtcc cgcatagatg cctatgcaga agatagccag
     1801 aaagtagatg acgatgacga aaaggtccgt gagcatgcct ggaaagagaa ggccttttcc
     1861 ggaatggaaa ttccggaaaa ggccccgggc ttgatgaagg gatcaagagt ggctcttgtg
     1921 ttccagcgcg gctgaaacca gaccggaaaa gagcgggtgg gcccggttgg gagcggactg
     1981 gaattccgga tggtactggc aggcgatgaa gaagggatgc tcgggaagtt ccacgacttc
     2041 caccagtccg tgttcgtcat tgacggcgct gatgaccagc cctgcgtttt ccagttcgtc
     2101 gcggtaggcc gggttgaatt cgtaccggtg gcggtggcgt tccgtgacgc tgtccttgcc
     2161 gtaaagcttg tgcgccagcg ttccgggccg gatcagggcc ttgtaggcgc ccaggcgcat
     2221 ggtggctccc atggcttgga tgcctttttg ttcttcctgg aggctgatga ctgggtgggt
     2281 ggttttctgg tcgaattccg tggagttggc gtcatccatg ccgcagatgt ggcgtgcgta
     2341 ctcaatgacg gccacctgca tgcccaggca aatgcccagg aacgggattc ctttggtgcg
     2401 cgcgtattcc accgtctgga tttttccttc aatgccgcgg tctccaaagc cgcccggcac
     2461 cagaatgccg tccacggagc caatcatggc ctccgcgccc ttttcctcaa tttcttccgc
     2521 gtctatccgg agaatttcca cgcgggcgtc attttccgca ccggcgtgag tgaaggattc
     2581 atagatggat ttgtaggcgt cctggagagc gatgtattta cctactacgg caatcctgat
     2641 ggagtggctg gggctgatga ccctgccgac gtaacgctgc cagtccgcca gattgggcgc
     2701 cggaacgtcc agtcccaggc gcctggtgac aatgcggtct attttatcct ggctgagatc
     2761 cagggggcat tcgtagatgg tgtttttgac gtcgcggaag gcaatgacgt ttttggcttc
     2821 cacgttgcag aacatggcga ttttgcggcg ttcgtcttca ctcatgtcgt attccgtccg
     2881 gcagatgatc acgtccggct ggatgccgat ttcccggagc ttggcgacgg attgctgggt
     2941 gggcttcgtt ttcatttccc cggcggcctt gatatagggg agcagggtga cgtgaatgaa
     3001 gcagacgttg tcacggccca cttccagggc gaattggcgc agggcttcca ggaaaatgtg
     3061 gccttccatg tccccgaccg tgccgccgat ttccgtgatg atgatgtcca cgtctgattt
     3121 ttccgtgact tcatagaggc ggtttttgat ttcatccgtg acatgcggaa tgtattgaac
     3181 cgtttttccc agatagtctc cccggcgttc cttccgcagc acgctttcaa agacctgtcc
     3241 ggaagtgagg ttgtttagcc gggagaggct gcaatggacg aagcgttcgt aatggcccag
     3301 gtccaggtcc gtttccgctc cgtcgttcaa gacgtacact tccccgtgct ggaacgggct
     3361 catcgttccg gggtccacat tcaggtaggg gtcgaatttt tgaagggtta ccttgagtcc
     3421 gcagcgttcc agcagcgtgc cgatggatgc cgccgccagt cctttgccga gagaggagac
     3481 aacaccgcct gtgacgaaga tatatttcat gatttgcttc ctagtgtatg tttaataaga
     3541 tttgttccac ctgttccgcc tgttccggcg tatccacgcc cacactgacg tggtccgtca
     3601 ggatgacccg gattctggcc ccgttttcca gtgcgcgcag ttgttccagg gattccgtct
     3661 gnnnnnnnnn nnnnnnnnnn ccaggggagt cggagcccag cgcacatagt tttcaagaaa
     3721 gtcgcggcgg taggcgtaaa tacccagatg gcgcaagggt ggcgctacgc gtggatggcg
     3781 ggcgtaggga atgacggaac gggagaagta cagggcgtct ccgttacggg caagggccac
     3841 tttgacaatg ttcgggttgt ccagatcctc ccgggcgctg atggggcagg cgacggtggc
     3901 catggagagg gcttcgtccg agaccagggc ttccgccagc ctgtcaatca gggccgggtc
     3961 aatgagaggt tcgtccccct gaatattgac gacatgggtg gcggcaggga aggaacgtac
     4021 cgcttccgcc aggcggtcac tgccgctggg atggtccgga gaggtcatga tggccctggc
     4081 tccaaaggat acggcggcgt caaaaatgcg ctgatcatcc gtggcgatgg cgatggcgtc
     4141 caggcgggaa cagcggcaga cgcgttccca tacgtgctgg acaagaggtt ttcctgcaat
     4201 gggatgaagg ggcttgcccg gaaatctgga ggaaccccag cgtgacggaa tgaggccgat
     4261 gatatgatgg gaagtatctg ctgccatggg aaaaggaacc gccccgaaag gtagcaactc
     4321 cctttgaatt agtccagcct caagcatagg ggccgcctgt ttttcccggg aaggaacagt
     4381 cagccggaaa tggatgccgt tgcggcggct tgccgttttt cctgcgtttt ccggatcatg
     4441 agcggcatgt attccttgcg gacatgaagg gatacataga ggccctccct cctggcgcag
     4501 gagtagagta ctttgatgat ggccagggat atgatgtcca tcgcaatggt gatgctgtcc
     4561 ccaaagatca ggaaaaagaa gattggtttg gtgaaataga cggcggaaac atacaggatg
     4621 ccgagcttgt attgatggtt gcggtaaatt ctactgacga tccatgcgta ggtcatgcca
     4681 acgacggaca ggataaggta caggaaaatc cctatgtagc cgagcgtttg aatcgtggaa
     4741 atggggccgc tgtgccattg gccgttccac gcaaaggcgc tttggtcttt gctcaggttg
     4801 taggcttctt catacacatt ggctttcacg atgctgatat cccgtgaaaa gccgtctccg
     4861 aatattttgt cctgaataaa gtgttcacgg tcatccagag cccagctcca catttcaaag
     4921 cgccagttga tggagtcttc ggcgtttgcc ttggcttgcg cgctgacgtc caggaaagga
     4981 acggctgaga gggtgcgctg gatgccgaaa ggcagagaat ggagcatgcc cgcagaggag
     5041 agcagcaaga gcaatagcac tccgaggacg ggagccagaa ggcagaagaa ccatcttctg
     5101 taaattaggg atatggtgaa gaacaggaac agaacctggg ccatggcgga gcgaaatccg
     5161 cttaacagga tgccaacggt tccaagaaga agcaacgccc cccaccaggg acgcttgatc
     5221 atctgccaca cggagtagtt gatgatgagc agctgcatca ccaagataga aattctgagg
     5281 aaggaagagt tccgggagtt ttcggagagt gtgcctgcgg agcccgcgct cgcggcaaga
     5341 tccgccccag cgctatccgg agaaaggagg ctccgggcgg ttaggataag ggtaaaaacg
     5401 atggtcagga aaacagccca ttgaagcacc ttgcccagtt catggctggt cgtttttaac
     5461 gaggacaggc agagataggc caggagggcc tggaggaaaa ggatgtatcc cttgcctccg
     5521 tagtagtctt ccagcacatc cagccccaga gcgaaagggt ggctcaggaa gatgtagccc
     5581 acatgcatga acagcacgag gacgacagcg tccagaaggg gggcggaatt ccacgtcaag
     5641 cttgaacgcc tgagcatggc gttccacaag taacaaggaa gggttattcc gtaagcaagg
     5701 aggggagcgt aatcccttaa ggaggggatg ggcaggaaaa gaatgggtat cagtatccac
     5761 agatacgaca ctttt
//
LOCUS       DAOMSU010000129         5761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1202_length_5761_cov_2.847354, whole genome shotgun sequence.
ACCESSION   DAOMSU010000129 DAOMSU010000000
VERSION     DAOMSU010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5761
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1202_length_5761_cov_2.847354"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            24..707
                     /locus_tag="PK077_06560"
     CDS             24..707
                     /locus_tag="PK077_06560"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HRN23999.1"
                     /translation="MLTLAQIHGQPEIFHSIQGEGVSQGTPCVFLRLAGCNLACSWCD
                     TAYSWNGTVPGVRLAPEKAAELVLHYPCRRLVLTGGEPLIQQKALPALLRLLPDHAVE
                     METNGTIMPDTELLKRVTQFNVSPKLPHSGNNDVKTWKPDTLRCLAGTEKAWFKFVVA
                     CEDDVRAVLRRASEADIPPERILIMPLASTQDELNAMRAQTVEWCLRYGLRFSDRLHI
                     AIWDSKKGV"
     gene            complement(801..2771)
                     /gene="rho"
                     /locus_tag="PK077_06565"
     CDS             complement(801..2771)
                     /gene="rho"
                     /locus_tag="PK077_06565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRN24000.1"
                     /translation="MSDTPPDLTPEQVSPEPAPKKRIIRKTKSAAEAPVKPQTAAEVS
                     GTEQDSSPAPRKRTIRKKAVELADDGMESVDVPGKKTSRKSGAEIPVEEAQEVPARPR
                     RGRPRKKPVEEVPETDGSAVVSGTEAAVKPVRRRAAKAVAPENGDSAGPEAEIAVHAA
                     PAEPASDSVPEQPAEGRPKVIRQRFVRKPREQEPEADASAPSIKVVQEGAADFSEDAA
                     REPRRTNEPQRQRFDRQNRRNNNDRFNKNRNNRQDGRNGRNGNDRVKNRWNNNQEGGT
                     PQNSAPRELAPPEPVDGLLEITNKGFGFLRKPDNDFDAFAEAVYVPQDMIRRFGLRPA
                     VWVHGQACRHDRGILLTEIASVNGVAPEKARKNPHFEELKAVNPNKRISFETRPERYT
                     TRTLDLIAPIGRGQRGLIVSPPRAGKTTLLQHMAEAILENYRDSIHLMVLLVDERPEE
                     VTEFKRSLPGAEVYASSNDGRVRDHCRMAELCIERAKRLVEAGQHVFLLMDSITRLAR
                     AYNNADKGSGRTMSGGIDARALEMPRRLFAAARNTRQAGSLTIIATALVETNSRMDDL
                     IFQEFKGTGNMELVLNRRIAEQYIFPAVDILKSGTRREELIMPEAWLYKMNLIRRALA
                     GHKPVEAMERFLFFLNKYPSNAQMLLDLKQKA"
     gene            complement(2823..3578)
                     /locus_tag="PK077_06570"
     CDS             complement(2823..3578)
                     /locus_tag="PK077_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRN24001.1"
                     /translation="MSGHNKWSKIKYVKAKEDAKKGKVFARFAHEIMLAAKSGGGDPD
                     LNPRLRAAIDGAKAVSTPKENIERAIKKGTGELGGATIQEITYEGYGPSGTAFLIEVA
                     TDNTNRSASELRTLFTKNGGSIGTPGSVAYQFERKGEARIMAEGLTEDSAMDLALECG
                     ADDVEQGDSDNEWVFVTSPTELNNVCAALREAGHTVISMKLISVAQNASVINDLETAK
                     AALRLYEALDDYDDALNVFSNFDVAEEILEQLG"
     gene            4115..5533
                     /locus_tag="PK077_06575"
     CDS             4115..5533
                     /locus_tag="PK077_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRN24002.1"
                     /translation="MEQAAHQEKSHAALSSVLWSAFLTLIKLWAGIVTGSLGIISEAL
                     HSGLDLMAAAMTFYAVKVASRPADESHPYGHEKVENLSALAETALLLVTCAWIVWEAV
                     DRLFYNEAEITLTWWAFAVVAVSLLVDVNRSAMLRRIAKKHKSQALEADALHFTTDIW
                     SSAVVLLGLFCIWLAHLVPAESAWHGLLEKADAIAALFVAALVCSVAFGLAKRSIHAL
                     MDGGSSSLTRQVLDAMKKNAPDYPVKRIRLRDGGARIFVELDVEAPAELHVDDAHDVA
                     ESIEGIVKHELPEADVIVHIEPARENLSHMEPDMIIHRLALRHHVRIHGFYAGHGKQA
                     PCYFMDVEIPADWPLERGYHVVEAFRDSILHELKPEKVICRIEPDCRDMETSAIAEQV
                     SPEEVRLKVNLILQQHRNIRKILKLDLTRENNFPTLTCVCSVDASLTIRECHQIASQL
                     ENQIENALHHLGRVTVILKPSK"
BASE COUNT         1219 a         1707 c         1535 g         1300 t
ORIGIN      
        1 gatagtctca ggccagcccg atcatgctca cactggccca gattcacgga caaccggaaa
       61 ttttccattc cattcaggga gaaggagtct cccagggaac accctgcgtc ttcctgcgtc
      121 tggcaggctg caatctggcc tgttcctggt gtgatacggc gtattcctgg aacggaacgg
      181 ttcccggagt gcgcctcgcg cctgaaaagg cggctgaact ggtgctccat tatccatgcc
      241 gccgcctggt cctgaccgga ggggagccgc tcattcagca aaaggcgctt cccgccctgc
      301 tgcgcctgtt gccggatcat gccgtagaaa tggaaaccaa cggcaccatc atgccggata
      361 cggaactgct gaaacgcgtc acgcaattca acgtatctcc caaactcccc cattcaggca
      421 ataacgacgt taagacctgg aaaccggata ccctgcgctg tctggcgggt acggagaaag
      481 cctggttcaa attcgtcgtg gcgtgcgagg acgatgtcag ggccgttctg cggcgggctt
      541 ccgaagcgga catccctccg gaacgaattc tgatcatgcc cctggcctcc acacaggatg
      601 agttgaacgc catgcgtgcg cagacggtag aatggtgtct ccgctacggt cttcgttttt
      661 cagaccgtct gcacatcgcc atctgggaca gcaaaaaggg cgtttgaaca cgcgcctgat
      721 tcaatcagga aagaccatgc tttccgcttt tccgaacgga attccgcaag cctcccaaag
      781 ctctgcgtca tttgggaagc ttacgctttc tgtttcaaat ccagcagcat ctgggcattg
      841 ctggggtact tgttcaggaa gaacaggaaa cgctccatgg cttctacagg tttatgcccg
      901 gctaaagcgc ggcggatcag attcatcttg tacagccatg cttccggcat aatgagctct
      961 tccctccgcg tccccgattt cagaatgtcc acggccggga agatatattg ctccgcaatg
     1021 cggcggttca gcaccagttc catattgccc gtccccttga attcctggaa aatcaggtca
     1081 tccatgcggc tgttggtttc caccagggcg gtggcaatga tagtcaggga accggcctgg
     1141 cgcgtattgc gggcggcggc aaaaaggcgc cggggcattt ccagagcgcg ggcatcaatg
     1201 ccgccggaca tcgtgcggcc gcttcccttg tcagcgttat tgtaggcgcg ggccaggcga
     1261 gtaatggaat ccatcagcag gaaaacatgc tgccccgcct ctacaagccg tttggcgcgt
     1321 tcaatgcaaa gttccgccat gcggcagtga tcgcgcaccc ttccatcatt ggaagaggcg
     1381 tatacttcag ctccggggag ggaacgttta aactccgtca cttcttccgg acgctcgtct
     1441 accagaagca ccatcaggtg aatggaatcc ctgtaattct ccagaatggc ttccgccata
     1501 tgctgaagca gcgtcgtctt ccctgcgcgg ggcggagaga caatcaggcc gcgctggccg
     1561 cgcccgattg gagcaatcag atccaatgtg cgggtggtgt aacgctccgg ccgggtttca
     1621 aaggaaatgc gcttattagg attgaccgcc ttgagctctt caaagtgcgg gttcttgcgc
     1681 gctttctccg gagcaacccc gttaacggag gcaatctccg tcagaagaat gccgcggtca
     1741 tgacggcagg cctgcccatg cacccacacg gcaggacgca aaccgaacct gcggatcata
     1801 tcctgcggca cataaacggc ttccgcaaaa gcgtcaaaat cattatccgg cttccttaaa
     1861 aagccgaacc ccttattcgt aatttccagc aacccgtcca ccggttccgg cggcgccagc
     1921 tcacgggggg cgctattctg aggggttccg ccctcctgat tattattcca gcggttttta
     1981 acccggtcat tgccgtttct gccattgcgg ccatcctggc ggttattccg gtttttgttg
     2041 aaacggtcgt tgttattccg gcgattctgc ctgtcaaaac gctgcctttg cggctcattc
     2101 gtccgacgcg gctcccgggc ggcatcttcg gaaaaatccg ccgcaccttc ctgcaccacc
     2161 ttgatggacg gagcggacgc atccgcctcc ggctcctgtt ccctcggttt cctcacaaat
     2221 ctctggcgaa taaccttcgg cctgccttct gcgggctgtt ccggaacaga atcggaagcc
     2281 ggctcggcag gcgccgcatg aacggcaatt tccgcctcgg ggccggctga atcaccgttc
     2341 tccggagcaa cagccttggc agcacgcctg cgcaccggct tgacggcggc ctccgttccg
     2401 gagacgaccg cagagccgtc agtttccgga acctcttcca caggcttctt gcgcgggcgt
     2461 ccgcgccggg gcctggcagg aacctcctgc gcttcttcca ccgggatttc agcaccgctt
     2521 ttacgggaag tcttcttccc cggcacatca acactttcca tcccatcatc cgccaattca
     2581 acagccttct tccttatcgt ccgcttgcgg ggagccgggg aagaatcctg ttccgtcccg
     2641 gatacttccg cagcagtctg gggctttacc ggagcttccg ccgcagactt ggttttgcgg
     2701 ataatacgtt ttttgggagc cggctccgga gaaacctgtt ccggagtcaa atctgggggg
     2761 gtatcagaca tggaaaagaa aattgaactg caacaacagg cgtctcctga aggacaacag
     2821 cgttaaccaa gctgctcaag gatttcttcc gccacatcga aattggagaa gacgttcagc
     2881 gcgtcatcat agtcgtccag cgcttcatac aggcgcaggg cggccttcgc cgtttccaga
     2941 tcattaatca cggaagcatt ctgcgccacg gaaatcagct tcatggaaat taccgtatgc
     3001 ccggcttcac gcagggcggc gcacacgttg ttcaactccg ttgggctcgt aacaaacacc
     3061 cattcattat cggaatcccc ctgttccaca tcgtccgcgc cgcattccag cgccagatcc
     3121 atggcggaat cctccgtaag accttccgcc atgatgcggg cctctccctt gcgttcaaac
     3181 tggtaagcca cggaaccggg cgtaccgatg ctgcctccgt ttttggtgaa cagggtacgc
     3241 agctcggaag cggaacggtt tgtgttgtcc gtcgccactt caatcaggaa agccgtgcct
     3301 gaggggccgt agccttcata ggtaatctcc tggatggtag ctcctcccag ttccccggtg
     3361 ccttttttga tggcgcgttc aatattttcc ttgggggtgg acacggcctt ggctccgtca
     3421 atggcggcgc gcaggcgcgg attcagatca ggatctccgc cgccgctttt ggcggccagc
     3481 atgatttcat gggcaaagcg ggcaaaaacc ttacccttct tggcatcttc cttagcctta
     3541 acgtacttga tcttagacca tttattatgt cctgacatag aattgggaat aatagtaagt
     3601 gtaggtgaaa agagacaaac taacaagtgg aactgttcta caacccgggg aacacattgt
     3661 catttgcaaa taccacgcag gaagggaatc agggtgcggc catccgtaaa tacgttacag
     3721 gtaccggata ctctgccggg gaacacgccg cgcaccttca ggagctccgg ctacctcttg
     3781 gcagtcactc tcaacacacg caaaataacg tattccccag cattggcaat acttttttag
     3841 gtcacggcaa ccggctgctt tcagagttat cttctaccgc cttcaattaa tttttaatat
     3901 attttgatta tttaaaataa taactactgt ttctttaata gcgaaaataa aaaaatatat
     3961 ccgcaccgca ggctcctgtt acgcatccag ccctgatggt gcagggggtg attccgtcat
     4021 gggccaaagc ctgagttcta acgccgcctc tgttatcatt gactaagaaa gggaggcggg
     4081 cttagatatg cccttctttc tttttccctg tcttatggaa caagcagcgc accaggaaaa
     4141 atcacatgcg gccctttcct ccgtcctgtg gtccgccttt ttaacgctca tcaagctctg
     4201 ggccggcatt gtaaccggca gcctcggcat catttccgaa gccctgcaca gcgggctgga
     4261 cctgatggct gcggccatga ctttttacgc cgtaaaagtg gcgtcccgcc ctgcggacga
     4321 aagccacccc tacgggcacg aaaaggtgga gaatctttcc gccctggcgg aaacggccct
     4381 ccttttagtt acctgcgcct ggattgtctg ggaggctgtg gaccgtctgt tctacaatga
     4441 ggcggagatt accctcacct ggtgggcttt tgccgtagtg gccgtctccc tgctggtaga
     4501 tgtgaaccgt tccgccatgc tccgccggat agccaaaaag cacaaaagcc aggccctgga
     4561 agcggatgca cttcacttta ccacggatat ctggtcttcc gcagtcgtgc tcctgggtct
     4621 tttctgcatc tggctggccc atctggtccc cgcggaatca gcctggcacg gcctgctgga
     4681 gaaggcggac gccattgcgg ccctgtttgt ggcggctctg gtttgttccg tggctttcgg
     4741 ccttgccaaa cgctccatcc acgccctgat ggacggcggt tcttcctccc tgacgcggca
     4801 ggtgctggac gccatgaaaa agaatgcccc ggattatccg gtcaaacgca tccgtctgcg
     4861 cgacggcggc gcgcgcatct tcgtagagct ggatgtggaa gcgcctgcgg aactgcatgt
     4921 ggatgacgcc catgacgtgg cggaatccat tgaaggcatt gtaaagcatg aattgccgga
     4981 agcggacgtc atcgtccata tcgaaccggc gcgggagaat ctttcccata tggagccgga
     5041 tatgattatc caccggctgg ccttgcgtca ccatgtccgc attcacggct tttatgcagg
     5101 ccacggcaaa caggccccct gttatttcat ggatgtggaa attcctgcgg attggcccct
     5161 ggaacgcggc taccatgtgg tggaggcttt cagggattcc attctgcatg agctcaagcc
     5221 ggaaaaggta atttgccgca ttgaaccgga ttgccgcgac atggaaacaa gcgccattgc
     5281 ggaacaggtt tctccggaag aagtgcgcct gaaggtaaat ctcattctcc agcagcacag
     5341 aaacatccgc aagatcctca agctggacct gaccagggag aacaatttcc caaccttgac
     5401 ctgcgtgtgt tccgtggacg cctccctgac tatccgggaa tgccaccaga tcgcctcgca
     5461 gctggaaaac cagatagaaa acgcactgca ccacctgggg cgcgttaccg tcattcttaa
     5521 accgtccaag tagaacagaa gttgaatgac tgttgccgga ttcccgtaaa aacatgttcc
     5581 gaatccggga aggggaagca gtttccattc cgggcatggg aacctccgcc cgaacaaagc
     5641 ggagccaacg attatgaatg gcccgcaacg gaaactccgg gcatttccct gtccagctcc
     5701 gcgcgaatgc gctgacgctc ggacacaatc tgttcgtcgt ccgcgccgcc ttcccgcagg
     5761 a
//
LOCUS       DAOMSU010000130         5688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1226_length_5688_cov_2.969466, whole genome shotgun sequence.
ACCESSION   DAOMSU010000130 DAOMSU010000000
VERSION     DAOMSU010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1226_length_5688_cov_2.969466"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            379..1392
                     /locus_tag="PK077_06580"
     CDS             379..1392
                     /locus_tag="PK077_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRN24003.1"
                     /translation="MSGPMMGHRSKAASALQRRISNNLRRILLTEQEILLSTSSGTGL
                     MEGAVRSCTAKRAAIFSVGAFGDKWYKIATGNGVPSDIFKSELGQPTTPEMVDAALST
                     GKYDTICITHNETSTGIQNPVEEIAEVLKKYPDVVWCMDAVSSAAGSRIETDKLGVDV
                     LVTSTQKALALPPGMAVCTLSQKAYERTASVPNRGCYFDLRSIYDTIQKKDYQYTNTP
                     CVSIMYAMDLQLQRIMQEGVENRFARHEAMAEFVRSWADEYFSVFANRDHLSRTLTVI
                     SNTRDIDIAALNNSLIERGMQLGNGYGDLKNKTFRIAHMGELTMDDMRSITSSIVDIL
                     KLK"
     gene            1722..2975
                     /locus_tag="PK077_06585"
     CDS             1722..2975
                     /locus_tag="PK077_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HRN24004.1"
                     /translation="MSTLHPFPALRPPRELAARVSSLPYDVMNHREAKEMAAGNDASF
                     LHICRSDIDTSEAAIHAPETYAKARENLERFVREGYLFRDERPSFYIYRQIMWGRVQT
                     GIVGCASVDEYASGAIKKHELTRREKELDRIEHFDACSAQTEPVFLAYRKHEGLSAII
                     REWIKFHKPEYDFTTEDGVTHILWPVSEPGTVEDIRKSFEEVDALYIADGHHRTASSA
                     AVSARRRQRHPDYTGQEEFNYLMAVVFCDEDLFIMDYNRVIRDLNGLTEEEFMEKLRT
                     SFDINPVDTIPGEGYAPRAKHEFGMYLNGRWYSATAKPGTFPAGHPIESLDCAILQAN
                     VLAPILGIDDPRTSDRIDFVGGIRGLGELERRCSGEMALAFSLYPVTMADLFRVADAG
                     EIMPPKSTWFEPKLRSGLFIHTIEQ"
     gene            2995..4566
                     /locus_tag="PK077_06590"
     CDS             2995..4566
                     /locus_tag="PK077_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase family protein"
                     /protein_id="HRN24005.1"
                     /translation="MNKVLIPTKLSDVAANTLKTAGYEVIQDADTPLEKQVAAHPDAV
                     ALIVRSEKVTPEIMDALPSLKLVVRAGAGYDNIDIVYARKKNVDVMNTPGANSNAVAE
                     EVMAMILAYYRHIVQADATTREGLWEKKKYMGSELTKKTVGIIGLGNIGRNLVKRLQG
                     FEPILLGYDHFLARQRALNIGVTPTSIEDIFSQCDIITLHVPGGPSTHHMVNAELIGK
                     MKDGAVLINCSRYGVVDEEALAAAKAAGKNIGYLTDVHPKDAPGEKPSAPIADLILPH
                     LGANTREANTKAAKRAAEQMIAYFSDGDTSCVVNGESPSSLNPAHLQLAFLLASLARK
                     AGGNKPIRRVECTFYGNLRIFRKWFTAPILEGLLPHAEKGLMPAAAEESLREHGIVFK
                     AREPKDDKPYEDSITLDVAMEEEEEYFHTSVRGVVTEGIPMVSRLNNFNGLYADLRGT
                     TLCLRYKDRPGIIALIGSALSSNGINIDNIAAPADHATREALTVIKTNQPVSDELLDK
                     IAKEIDAISAFSLNL"
     gene            4711..>5688
                     /locus_tag="PK077_06595"
     CDS             4711..>5688
                     /locus_tag="PK077_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24006.1"
                     /translation="MRYVLLLLTLLTAGFSVWYYQNRISPEGKELAAQKTTLEQEAAI
                     LEKAVIQSRKKLDDAQDATRAAEEDIERFKQAYLQQKRQQHQEIQDAAYQKSVADQQE
                     EVKEHDQKIDRLRRRMKRQRESTKSAREELLRKKEGMRTQLDRLTDQQRSIQAELEQA
                     ERELAQKREEAKSRKKLSGIAPQKGNISELQTQMNLIRQKTAAAKEKMKAVDAALETL
                     EEKSGKQERLMQKAEEKLTEQKNKVLADDDRDESAVPEPGDEDLLAAAEYRELSRDVR
                     KTLEQARKKEEQASRAYDEARAAMRKASREEVKKLAKKEKDHASFQKLFT"
BASE COUNT         1416 a         1731 c         1460 g         1081 t
ORIGIN      
        1 gaaaaaagcc gtttcaccgg cagaacgtga gaaccgtttc agcacaggtt caccgcctcg
       61 ttccatcctg atttcctgcc agaaaatccg gttgaaaagg atcaccggag gaaccatgcc
      121 ggcatgggat gacggaacat gcggagcgcc gcatcctgcc cgcattactc ccaactggcc
      181 acctgtgccg cccaatacag acatcctctc tttgctttct ccgctgctgc ggcaggtttt
      241 cccggtaagg ctcaacaaaa attcatagtc aaaccgtttg gagacgctat agtatgcgta
      301 cccagttctt aaaaacaaat ggcaaccaaa ttattcattc ccggacccac tgaagtagca
      361 cctgaagtac ttgccgccat gagcggccca atgatgggac accgatccaa agcggcctcc
      421 gccctgcagc gccgcatttc caacaatctg cgccgcatcc tgctgacgga acaggaaatt
      481 ctgctttcca cttcctccgg tacgggcctg atggaaggcg ccgtgcgttc ctgcaccgcc
      541 aaacgcgccg ccattttttc cgtgggcgcc ttcggcgaca aatggtacaa gatcgctacg
      601 ggcaacggcg tgccctccga tattttcaag agcgaactgg ggcagcccac taccccggaa
      661 atggtggacg ccgccctctc caccggcaaa tacgacacca tttgcatcac gcacaatgag
      721 acgtccaccg gcatccagaa cccggtggag gaaattgcgg aagtgcttaa aaaatacccg
      781 gatgtcgtgt ggtgcatgga cgccgtgagt tccgccgccg gctcccggat tgaaacggac
      841 aagctgggcg tggacgtact ggtgacctcc acccagaagg cgcttgccct tccccccggc
      901 atggctgtct gcacgctatc ccaaaaagcg tacgaacgca ccgcctccgt tcccaaccgc
      961 ggctgctact ttgacctgcg ttccatttac gacactattc agaagaagga ctaccagtac
     1021 acgaacactc cctgtgtctc catcatgtac gccatggacc tccagctcca gcgcatcatg
     1081 caggaaggtg tggaaaaccg ctttgcgcgt catgaagcca tggccgaatt cgtgcgttcc
     1141 tgggcggatg aatatttcag cgtctttgcc aaccgggatc acctttcccg caccctgacg
     1201 gtgatcagca acacgaggga cattgacatc gccgccctga acaactccct gattgaacgc
     1261 ggcatgcagc tcggcaacgg ttacggggac ttgaagaaca aaacgttccg cattgcccac
     1321 atgggcgagc tgacaatgga tgacatgcgc tccatcacgt ccagcatcgt ggatatcctg
     1381 aagctcaaat aagccaggcc cctgccctct tttccggctc cgtccggaaa agagggcgga
     1441 acgcctccgc cggaacatga acggaacaat cgccgggaac aggatacatc gcggatatcc
     1501 cggaacttgt tcatctttga tcttttccgc atcccggcgg cattcgtcat cgtccaaact
     1561 ttcaatcagg cacggtcttt ccgggacaac ccgttctttc aggactccat ttcacgttat
     1621 ttgctgctgt tcattgcggc atgcattttg gccgggcttg tttccagcat tcgtgcgcta
     1681 tatcatcctt cccattactg actttccgaa ctcttccttc catgtccact ctccatccct
     1741 tccccgccct gcgccctcct cgggaactgg ccgcgcgggt ctcctccctc ccctatgatg
     1801 tcatgaacca ccgggaagcc aaggaaatgg ccgccggaaa tgacgcctct ttcctccata
     1861 tctgccgttc cgacatcgac acgagcgaag ccgccatcca tgccccggaa acctacgcca
     1921 aggcccggga aaacctggaa aggtttgtcc gcgaaggtta cctcttccgg gatgaacggc
     1981 cgtctttcta catctaccga caaatcatgt gggggcgcgt acagacgggc atcgtcggct
     2041 gcgcctccgt ggatgaatac gccagcggcg ccattaaaaa acacgaactg acccgcaggg
     2101 aaaaggaact tgaccggatt gagcattttg acgcctgctc cgctcagacg gaaccggtat
     2161 tcctggccta ccgcaaacat gaaggtcttt ccgccatcat ccgggaatgg attaaattcc
     2221 acaaacccga atatgatttt accacggagg acggcgtcac ccatatcctg tggcccgttt
     2281 ccgagcccgg cacggtggaa gacatccgca aaagctttga ggaagtggac gccctttaca
     2341 ttgcggacgg gcaccaccgc acggcttcca gcgccgccgt ttccgccagg cgccgccaga
     2401 gacacccgga ctacacggga caggaagaat tcaactacct gatggctgtc gtcttttgtg
     2461 atgaagacct tttcatcatg gactacaacc gcgtcatacg cgacctgaac ggcctgactg
     2521 aagaggaatt catggaaaaa ctccgtacct cctttgacat aaatcctgtg gataccattc
     2581 ccggagaagg ctacgccccc cgcgccaagc atgagttcgg catgtatctg aatggacgct
     2641 ggtattccgc cacggccaaa ccgggaacct tcccggccgg ccatcccatc gaaagcctgg
     2701 actgcgccat cctccaggcg aatgtgctgg ctcccattct gggcattgac gatccacgca
     2761 cgagcgaccg catcgacttc gtaggaggaa tccgcggtct gggagaactg gaacgccgct
     2821 gttccgggga aatggcgctg gctttctccc tgtatcccgt caccatggcc gaccttttcc
     2881 gcgtagcgga cgccggagaa atcatgcctc ccaaatccac ctggtttgaa cccaagctgc
     2941 gcagcgggct ttttatccat accattgaac aataatcttt tccaccgtac caacatgaac
     3001 aaggtcctta ttcctaccaa actctccgac gtagcggcca acacgcttaa aacggccggc
     3061 tatgaagtca tccaggacgc tgatacgcct ctggaaaaac aggtagccgc ccatccggat
     3121 gccgtggctc tgatcgtccg cagcgaaaaa gtcactccgg aaatcatgga cgcccttcct
     3181 tccctgaagc tggtcgtccg cgccggagcc ggttacgaca acattgacat tgtgtacgcc
     3241 cgcaaaaaaa acgtggacgt catgaatacc cccggggcca actccaatgc tgtggcggaa
     3301 gaagtcatgg ccatgattct ggcctactac cgccacatcg tccaggcgga cgccaccacc
     3361 cgggaaggcc tgtgggaaaa gaaaaaatac atgggcagcg agctgaccaa aaagacggtg
     3421 ggcatcatcg gcctaggcaa catcggccgc aacctcgtca agcgtctgca gggatttgaa
     3481 cccattctgc tgggctacga tcactttctg gcacgccagc gcgctctgaa catcggcgtc
     3541 acccctacca gcattgaaga tattttctcc cagtgcgaca ttattaccct gcacgttccg
     3601 ggcggcccct ccacccacca catggtgaac gcggagctca ttggaaaaat gaaagacgga
     3661 gcagtactca tcaactgctc ccgctacggc gtggtggatg aagaagcgct ggccgccgcc
     3721 aaggccgcag gcaaaaacat cggctacctg acggacgtac atcccaagga cgcgcccgga
     3781 gagaagcctt ccgcccccat tgcggatttg attcttcccc acctgggagc caacacccgg
     3841 gaggccaata cgaaagcggc caaacgcgcc gcagaacaga tgatcgccta cttttccgac
     3901 ggcgacacct cctgcgtggt gaacggggaa tcccccagca gcctcaatcc ggcccatctt
     3961 caactggcgt ttctgctcgc ctccctggcg cgcaaggccg gcggcaacaa gcccatccgc
     4021 cgcgtggaat gcacttttta cggcaacctg cgcatttttc gcaaatggtt tacggccccc
     4081 attctggaag gccttctgcc gcatgcggaa aaagggctca tgcctgctgc cgcggaagaa
     4141 tccctgcggg agcacggcat cgttttcaaa gcgagggaac ccaaagacga caaaccttat
     4201 gaagattcca tcacgctgga cgtggccatg gaagaggaag aggaatattt ccacaccagc
     4261 gtgcgcggcg tggtgacgga aggcatcccc atggtctccc gcctgaacaa tttcaacgga
     4321 ttgtacgcgg acctgcgcgg cacgacatta tgcctgcgct acaaggaccg cccgggtatt
     4381 atcgccctca tcggctccgc cctgtcttcc aatggaatca acattgacaa catcgccgct
     4441 ccggccgacc acgccaccag ggaggccctg accgtgatta agaccaacca gccggtttcc
     4501 gatgagctgc tggataaaat cgcgaaagag atagatgcca tctctgcttt ttccctgaat
     4561 ctttaacagg gaaagacgtg tttccggaaa cctgcttttc aggccgcccg cttccttcat
     4621 ctccggaggg aagcgggcgg ttcttatttc tgccatgttg acaaggctta cccattttta
     4681 taccattgac cgaatcgtcg gaacatatcc atgcgttacg tactgcttct cctgaccctg
     4741 ttaaccgccg gattttccgt ctggtactat caaaaccgca tttccccgga aggaaaggaa
     4801 ctggccgccc aaaaaacaac attggaacag gaggccgcaa tattggaaaa agccgttatc
     4861 caatcccgaa agaagctgga tgatgcccag gatgccaccc gggcggcgga agaagacata
     4921 gagagattca agcaagctta tctccaacaa aaaaggcaac agcatcagga aatccaggat
     4981 gccgcctacc aaaaaagcgt ggcggaccag caggaagaag tgaaggaaca cgaccaaaag
     5041 attgaccggc tgcgccgccg catgaaaagg caaagggaaa gcacaaaatc cgccagggag
     5101 gaactgctcc ggaaaaagga gggaatgaga acccagcttg acaggctgac cgatcagcag
     5161 aggagtattc aggcggaact ggaacaggcg gaacgcgaac tggcccaaaa acgggaagag
     5221 gccaaaagca ggaagaaatt gtccgggatt gcccctcaga agggaaatat ttccgaactt
     5281 cagacacaga tgaaccttat ccgccagaaa accgcagctg ctaaggaaaa aatgaaagcc
     5341 gtagatgcgg cgctggaaac tctggaagaa aagtctggca aacaggaacg cctgatgcag
     5401 aaggcggagg agaaactcac cgaacagaaa aacaaggttc tggccgacga tgaccgggac
     5461 gaatccgctg tcccggaacc gggcgatgaa gatttgctgg cggcggccga atacagggag
     5521 ctgtccagag atgtacggaa aactctggaa caggcccgta aaaaagaaga acaggcatcc
     5581 cgggcctatg acgaagcccg cgcggccatg cggaaagctt ccagggaaga agtgaaaaag
     5641 ctggcaaaga aggaaaaaga ccatgcctcc tttcagaagc tgttcacg
//
LOCUS       DAOMSU010000131         5621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1254_length_5621_cov_2.688825, whole genome shotgun sequence.
ACCESSION   DAOMSU010000131 DAOMSU010000000
VERSION     DAOMSU010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5621
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1254_length_5621_cov_2.688825"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(238..987)
                     /locus_tag="PK077_06600"
     CDS             complement(238..987)
                     /locus_tag="PK077_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRN24007.1"
                     /translation="MNEHPGNTPMFQISHLVKEFNSVPAVNDVTTNIDRGEVVFIVGP
                     SGSGKSTFLRCLNLLEEPTSGEIRFKGKLVTSPGTDVNQFRQHVGMVFQHFNLFPHLT
                     ILENITIAPVKTGRSSRKEAADQAETLLQRIGLYGKRHSYPLQLSGGQKQRIAIVRAL
                     VMNPDVILFDEPTSALDPEMVGEVLSLMKELAQGGLTMVVVTHEIGFAREVASRVLFM
                     DKGKIVEEGPPDRVIDRPANPRLQEFFSKVL"
     gene            complement(984..1319)
                     /locus_tag="PK077_06605"
     CDS             complement(984..1319)
                     /locus_tag="PK077_06605"
                     /inference="COORDINATES: protein motif:HMM:NF022379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HRN24008.1"
                     /translation="MTDTVEDVRKGLPSASAFGRLALCPGSFTMEKSCPDESSEAAAE
                     GTLLHRYMEYLLLKDDAAEEGMGFSWHDFLNSREYESAELNHEQVELCGRALRLLDAR
                     QTGKKTEKQ"
     gene            complement(1335..2213)
                     /locus_tag="PK077_06610"
     CDS             complement(1335..2213)
                     /locus_tag="PK077_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24009.1"
                     /translation="MNTPTTESLTLQEQGQQLSVLGAFANSEQFQMAKQAAEMLASSS
                     MVPTTYQNNPGSCFIALNTALRLRMDPLMIMQNLYVVQNRPSWSGQFAIALVNICPKF
                     SATWFEYRNEEDFQKGVRMCAQLKTGQNVYGTWITPEMVKAEGWGKKWQTMPEQMYKY
                     RAAAFFARTNCPEALLGLSVEGEAEDMAGKSQPDIKPPLFKSREISGGDVVDAEKVAD
                     SPRQLGDAEVPGKGDVEVPPPHIRLMEELSCTEDQLNKAIAKASGDKVKGWHELNDKQ
                     KEKLAANPDKLQPFIG"
     gene            complement(2285..3109)
                     /locus_tag="PK077_06615"
     CDS             complement(2285..3109)
                     /locus_tag="PK077_06615"
                     /inference="COORDINATES: protein motif:HMM:NF021574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HRN24010.1"
                     /translation="MDNLNEETLAAVRVQEVANGRAAVVPDGYTLYHLDCLGNTPPRK
                     AGSVQLLDLETLADFVKAEDAENGVRSVIYVSDREVCAVLNYYSPDGNGWGDHQATMQ
                     LNKTVEWENWTKYDGQAMSQKDFVEFLEENSKDVMEPTPSAMLTLASKFDMHRKVEFK
                     SAYRASDGETKLTYNETVDSKSGELNVPTEFTIAIPVIRGAEGDTTYQIKVRLRVRLA
                     DGKLYFVYQLVRADIPERNAIKDIADKLAKDLPENRIHRGAVCMCTKSSFTGEIDR"
     gene            complement(3160..3555)
                     /locus_tag="PK077_06620"
     CDS             complement(3160..3555)
                     /locus_tag="PK077_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24011.1"
                     /translation="MSEVTKRQVPGDVFFEGLSEINEGALLEALDTKMTSLVSAVLAT
                     GNNGSLTLKLNVKRKGGVNQVVIEPKVTASIPDPTIAPRIMFADTSGALHTDDPAQGK
                     LDLDAPVKVTFPAAADVDAADTPRKVKQA"
     gene            complement(3564..3779)
                     /locus_tag="PK077_06625"
     CDS             complement(3564..3779)
                     /locus_tag="PK077_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24012.1"
                     /translation="MNTFFKFLGACSFGLSAACLFWLSVELDNAELQAGKSPHSGFCP
                     ESPTPMKAFDGLEKPSRPTRIVESNNQ"
     gene            complement(3776..4072)
                     /locus_tag="PK077_06630"
     CDS             complement(3776..4072)
                     /locus_tag="PK077_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24013.1"
                     /translation="MPDSDEAQAIAAFRGKVVVTLDDVRFMTGWGRERILALVQDGSI
                     QALPGTGSAGCPYEFPALSVWRYIHQQDHAQKPQVNGVDMNILPPRRRRKGAAA"
     gene            complement(4065..>5621)
                     /locus_tag="PK077_06635"
     CDS             complement(4065..>5621)
                     /locus_tag="PK077_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24014.1"
                     /translation="ILYNDSRKLARTKFNQVKFNGDESSSGFLESSIARGKRAFESGL
                     RNLEHLGEAEAASNVAARMPDKIAAGLSMEEARKEIEKDRAFLAIKRSWEKDLVQTME
                     EAEDDYLNSPERSVLQRVSSKLGGIVGDTSTFLVPYGGLFKAASLGRMAGAARLGGAA
                     TKFGVAGGSLMQRLREEGTMMGLDMLENEQRAGLFGAADFMEEMIAFGAMGRMVPGFG
                     RLTKWAGRGKPASWRARFASSPKAQLAAHTLMGVAEEALLEPTAGFIMRSAEGIFLND
                     ERGKATFSGYIQDMQAMLEPEQFAALALFSIGMSSLNYGQLSRDSARFRDMLTAYKDS
                     GGTEAGLLDAMKQPDMKSVLRRATENLHRELLENPQAARERAREAGREMLSGERIESL
                     RELDAWRAAEDAGMVPRVEPAEQEGMFRVYAPARSTKAPREDASVSREGQEENAPSYT
                     LMDGEQMTAYLQAFVSEQVESDILYTQHLLAGDVTVNQALAQGRFDAAEVITRTVTDE
                     KTGAERVVIA"
BASE COUNT         1195 a         1702 c         1388 g         1336 t
ORIGIN      
        1 cgggaagagt tgctgcgtga aaacaggggc aatcttgaaa aatgcaggtt ctatgactgt
       61 gactatctgt tgattgacga gtcttataac gtggagatca tgttgtagag aacagagact
      121 ttccattgtc cggggaagga aagagaaggt tatgccaaat tgctttgttt ataggcgcag
      181 gcggtaagag agaggttctt ccccccttcc cctgtatttt ttccagaact gcccgtgtca
      241 gagcaccttg gaaaaaaact cctgcagcct gggattggcg gggcggtcga taacgcggtc
      301 cggaggccct tcttccacaa ttttcccttt atccatgaac aggacgcggg aagcaacctc
      361 ccgcgcgaac cctatttcat gggtaaccac taccatcgtc agtcctccct gcgccagctc
      421 cttcatcagg gagagcactt ctcccaccat ttccgggtcc agggctgatg tcggctcgtc
      481 aaacaggatg acatccggat tcatgaccag ggcgcgcacg atggcgatgc gctgcttttg
      541 gcctccggaa agctggaggg gataggagtg cctcttgccg tacagcccaa tgcgctggag
      601 aagggtttcc gcctggtctg ctgcctcctt tctgctggag cgtcccgttt taaccggggc
      661 gatggtgata ttttctagaa tggtcaggtg cgggaaaagg ttgaaatgct ggaaaaccat
      721 gcctacgtgc tggcggaatt gattgacgtc cgttccggga gaggtaacca gctttccctt
      781 gaaacggatt tctccggagg tcggttcctc cagcaggttc aggcagcgca ggaaggtgct
      841 tttaccggaa ccggacgggc ctacgatgaa gaccacttca ccccggtcaa tgttcgtggt
      901 gacatcgttg actgccggca cggagttgaa ttccttaacc aaatggctga tttggaacat
      961 gggagtgttt ccgggatgtt cattcattgt ttttcagttt tctttccagt ttgccgagca
     1021 tccagcagac gcagagcacg tccacaaagc tccacctgct catgattcag ctcggcagac
     1081 tcatactccc ggctattcag aaaatcatgc caggaaaacc ccattccttc ttcggctgca
     1141 tcatccttca aaagaagata ctccatatag cggtgcaaca gcgtgccttc tgcggcagcc
     1201 tcggaactct catcgggaca ggacttctcc atcgtaaacg agccggggca taaagccagc
     1261 cgtccaaatg cggacgcaga gggcagccct ttacgtacat cttcaacagt atcggtcatg
     1321 ttgtcgtctc cttcctatcc gataaagggt tgcagcttgt caggattggc cgccagcttt
     1381 tccttctgct tgtcattcag ttcgtgccat cccttcacct tgtcgccgct ggccttcgca
     1441 atagccttgt tcagttgatc ttccgtacag gaaagctctt ccatcagccg gatgtggggc
     1501 ggcggaactt ctacgtcgcc tttgccggga acctccgcgt ctcccagctg ccgcggggag
     1561 tcagcaacct tctcggcatc cacaacgtcg cccccggaaa tctccctgga cttgaacagg
     1621 ggcggcttaa tatccggctg gctcttgccg gccatgtcct ccgcttcccc ctccacactc
     1681 aagcccagca acgcttccgg gcaattcgtc cgggcgaaaa aagccgcagc acggtacttg
     1741 tacatctgtt ctggcatcgt ctgccacttc ttcccccatc cttcggcctt caccatctcc
     1801 ggggtaatcc aagttccgta aacattctgc cccgttttca gctgggcaca cattctcacc
     1861 cccttctgaa aatcctcttc attacggtac tcgaaccaag tcgccgaaaa cttcgggcag
     1921 atattcacaa gagcaatggc aaactgtccg gaccaggacg ggcggttctg aaccacgtaa
     1981 agattctgca tgatcatcaa aggatccatc cgcagccgca gggctgtatt cagggcgatg
     2041 aagcaggaac cggggttatt ctggtaggtg gtcggcacca tgctggaaga tgcaagcatc
     2101 tcggcggcct gcttcgccat ctggaactgt tcactgttgg caaacgctcc cagtacggac
     2161 agttgctgtc cttgctcttg taaagtcagg gattctgttg taggggtatt catgttattg
     2221 gtattcacat tcatgttatt gataacaggc cggggaccag ttggcgctgg ccccggccaa
     2281 ctcactatcg gtcgatttct ccggtgaagg aggattttgt gcacatgcac acggcgccgc
     2341 ggtgaatccg gttctccggc aaatcctttg ccagcttgtc ggcaatatcc ttaatcgcat
     2401 tgcgttccgg gatgtccgcg cggacaagct ggtacacaaa atacagcttc ccgtcagcca
     2461 ggcgcacacg caggcgcacc ttgatttgat acgtggtatc tccttcggcg ccccggataa
     2521 ccggaatcgc aattgtgaac tcggtaggaa cattcagttc gccgctcttg gaatccaccg
     2581 tttcgttata agtcagcttc gtttcgccgt cggatgcccg gtaggcagac ttaaactcca
     2641 ccttgcggtg catgtcgaac ttgctcgcca acgtgagcat tgcagacggg gtgggctcca
     2701 tcacgtcctt gctgttctct tcaaggaatt caacaaaatc cttttgagac atagcttgtc
     2761 cgtcgtattt ggtccaattc tcccactcca ccgtcttgtt gagctgcatg gtggcttggt
     2821 ggtcccccca tccattacca tcgggggaat aataattgag cacggcgcat acttctctgt
     2881 cgctcacgta aatcacgctc ctgacgccat tttcggcatc ttccgccttc acgaaatctg
     2941 ccagcgtttc caggtccagc agctgaacac tgccggcctt gcgagggggc gtattgccca
     3001 ggcaatccag atgatacagg gtatatccat ccggcacgac ggcggcacgg ccattcgcca
     3061 cttcctgcac gcggacggct gccagagttt cttcgttcaa gttatccata taatttaatt
     3121 ctataatgtt tatgttgtta tacagtgttg ttggaaaact taagcctgct tcaccttcct
     3181 gggagtatcg gcggcatcaa catcggcagc agccgggaat gtcaccttca caggagcatc
     3241 caggtccagt ttcccctggg cggggtcgtc cgtatgcagg gcgccggagg tatcggcaaa
     3301 cataatgcgc ggggcaatcg tcggatccgg gatgctggcc gtaaccttgg gttcaatgac
     3361 tacttgattc acgccgcctt tgcgcttcac attcagctta agagtcaggg aaccattatt
     3421 cccggttgcc agcacagcgg aaacaaggct ggtcatcttg gtgtccaagg cttccaaaag
     3481 agccccttcg ttaatctcgg aaagtccttc gaaaaagaca tctccgggta cttgtcgttt
     3541 agttacttca ctcatgatgc tatctattgg ttattgcttt ccacgatccg cgtggggcgg
     3601 gacggttttt ccaaaccgtc aaaagctttc atgggagtgg gagactccgg gcaaaacccg
     3661 gaatgcgggc tcttgccggc ctgcagctcg gcgttgtcca gctccaccga cagccagaac
     3721 aggcacgcag cggaaagacc aaaggagcag gcccccaaga acttgaaaaa ggtattcatg
     3781 ccgcagcccc ctttcttctt ctgcgcgggg gaagaatatt catatccact ccattcactt
     3841 gaggcttctg tgcatgatcc tgctggtgga tatagcgcca tacagacagg gcagggaact
     3901 cataggggca tccggcgctt cctgttccgg gcaacgcctg aatgctgcca tcctggacaa
     3961 gggcaagaat gcgctctctt ccccagcccg tcatgaacct tacgtcatcc aaagtgacaa
     4021 ctaccttgcc gcggaaggcg gcaatcgcct gcgcctcgtc ggaatcaggc aatcaccacc
     4081 cgttcggctc cggtcttctc atccgtcact gtgcgcgtga tcacctccgc cgcgtcaaaa
     4141 cgcccctggg ccagggcctg gttcaccgtc acgtccccgg ccagcaaatg ctgcgtgtag
     4201 aggatatcac tctccacctg ctcgctcaca aacgcctgta aataagccgt catctgctcg
     4261 ccgtccatca acgtgtaaga aggggcgttc tcttcctgcc cctctctgga gacggaagca
     4321 tcctcccgcg gcgctttcgt gctgcgcgct ggagcataca cccggaacat cccctcctgt
     4381 tccgccggct ccacccgcgg caccatgccg gcatcctcgg cagccctcca cgcgtccagc
     4441 tccctcaaag actcaatgcg ttcgccgctc aacatctccc gtccggcttc cctggcgcgc
     4501 tccctggcag cttgcgggtt ctccaacaac tccctgtgta aattttccgt cgccctccgc
     4561 agcacgcttt tcatgtcagg ctgcttcatg gcatccagca atccggcctc cgtcccgccg
     4621 gaatccttgt aagccgtcag catatcccgg aaccgtgcgc tatccctgct taactgtcca
     4681 taattcagag aggacatgcc aatggaaaat aaggcaagag ccgcgaactg ctcaggctcc
     4741 agcatggctt gcatatcctg aatgtatcct gaaaaagtgg ccttccctcg ctcgtcattc
     4801 aggaatatgc cttccgcgct ccgcatgatg aaccctgccg tgggctcaag aagggcttcc
     4861 tccgccacgc ccatcagcgt atgagccgcc agttgcgctt tgggagaaga cgcaaacctt
     4921 gcccgccatg aagcaggttt cccgcgtcca gcccattttg tcaatctccc aaaacccgga
     4981 accatcctgc ccatggcgcc gaaagcaatc atctcctcca tgaaatccgc tgcaccaaac
     5041 aagccggcgc gctgttcgtt ttccagcata tccaacccca tcatggtccc ctcttcccgc
     5101 agcctttgca tcaaggagcc tccagcgacg ccgaacttgg ttgcagctcc acccaaccgg
     5161 gcggctccag ccatcctgcc aagacttgca gccttaaaaa ggccgccata aggaacaaga
     5221 aaggtggacg tatcccccac aattcctcca agcttggaag atacacgctg aagaacgctc
     5281 ctttccggac tgttgagata atcatcctcc gcctcctcca tcgtttgaac aagatccttc
     5341 tcccagctgc gcttaatcgc cagaaaagcc ctgtcctttt caatttcctt tcgcgcctcc
     5401 tccatactca atcctgccgc aatcttatcg ggcatcctgg cggcaacatt tgaggcggct
     5461 tctgcttcac ccaaatgttc cagattgcgg aggccactct caaaagctcg tttcccacgc
     5521 gctatggaag actccagaaa accggaagaa ctttcatctc cattgaactt tacctgatta
     5581 aacttggtac gcgccagttt tcgggaatca ttatacaaaa t
//
LOCUS       DAOMSU010000132         5599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1262_length_5599_cov_2.754149, whole genome shotgun sequence.
ACCESSION   DAOMSU010000132 DAOMSU010000000
VERSION     DAOMSU010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1262_length_5599_cov_2.754149"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(635..1762)
                     /gene="dprA"
                     /locus_tag="PK077_06640"
     CDS             complement(635..1762)
                     /gene="dprA"
                     /locus_tag="PK077_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRN24015.1"
                     /translation="MTPREAAIALNLIPGLGPVRIMRLLQVFASPELILESPSSLLME
                     IPGVGAQLACRISSWRSIVNPYRELELADNAGAAVTTVFDDSYPSSLRALPDPPIVLY
                     SWGNWTGTDAERSIAVVGSRMATHYGRLCARNISHDLAEAGVTVISGLARGVDTEAHT
                     GAMDAEGRTIAVIGAGLNKLYPRENRNLAQRIADGHGAVVSEFPMDLPPSRTTFPMRN
                     RIVSGWSRATLVVEASGRSGALITARTAAEQGRDVFCIPGPVDRHSSDGCHALIRDGA
                     ILATGASDILQDMNWAVPEQGLPLFSPCPPSGASTPPLPTLEEKEIFHAIRLGFNTID
                     TLCTSLGKAAHTITPLLAKMQIAGQITPDAGGYFSINSREL"
     gene            complement(1759..2871)
                     /locus_tag="PK077_06645"
     CDS             complement(1759..2871)
                     /locus_tag="PK077_06645"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRN24016.1"
                     /translation="MKIVHLVPSMESGGVEQVVMELGSGLSSRGVENIVVSGGGRLVP
                     RLEKEGSRHILMPIGKKSISTLFRIGALRALLQAVRPDILHLHSRVPAWAGYLAWKKL
                     PPEDRPGLVTSVHGFYSVNRYSAIMSRGERVIAVSNCIRDYILDHYPSTPPDHIRIIP
                     NAISPDQYHPAYSPSREWLTGWFMSYPELKGKFTLCLPGRITRLKGHLDLIPVVRQLL
                     EQGIPAHAVIVGEAKKGKEEYKNEVLRAIERSGVSQSFTWTGHRQDLREILSTCSVTL
                     SLTKSPEAFGKSTLEALALGKPVAGYAHGGVKEQLDAFLPEGNVAVGDTAAMADLLAR
                     WHAQPPPLPRQIPSPYNMQDMIQAHLDVYQELTPYS"
     gene            complement(2868..3791)
                     /locus_tag="PK077_06650"
     CDS             complement(2868..3791)
                     /locus_tag="PK077_06650"
                     /inference="COORDINATES: protein motif:HMM:NF018017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HRN24017.1"
                     /translation="MNIRILSDGKQGHLNQSLGLAQALISKAGGTVETVNLQGLSLLG
                     KIRKVVTGSDIPRPDLFISAGHATHIPLICARHHFKTRAILCMKPTLPCSFFDLCLIP
                     RHDLDSGRDYTDTDIFPTQGALHPMRPDTSVPKDTTLILIGGPSKDFDWDDESMLNQL
                     ASISIHTPGDLVLTTSRRTPDGFAEKIRTAVPELTVVPVEETRPGWVARHLAHASAAW
                     VSQDSVSMVYEALGSGAPVGILSVPRRHGSRKSRILSGLETLEKEGMVTGYSDWKKQN
                     FRLTAPGSPLLEADRAADYILSRFFPQLRAL"
     gene            3939..4955
                     /gene="queG"
                     /locus_tag="PK077_06655"
     CDS             3939..4955
                     /gene="queG"
                     /locus_tag="PK077_06655"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRN24018.1"
                     /translation="MSRQLGFSGCRVARAEKSPHAEKLFQWLERGWHAGMEWMARSPE
                     RRTDPAEVLPGCRSVICLSYDYDSPGRRPEGEGSICLYAHGKDYHGILEEKLADLQEL
                     LSIYGGKQRGYVDSGPVMERDHAEACGLGWRGKSGLIVRRKGGSRFFIATLLTTLELE
                     PDAPVSNGCGSCRRCAALCPTGAIMENGQVDAGRCLSYWTIEHRGAIPEEIRPLIGTR
                     LYGCDTCVTVCPWNGKPLPDVDERFRMSRLLASIPLRGLLSLDAGGFAALFRNSPLKR
                     LKREGLLRNGCVVLGNAGTPDDIDFLKALYGESPLVEEHASWAVERILRRHGRHASSS
                     GARD"
     gene            5074..5451
                     /gene="rpsM"
                     /locus_tag="PK077_06660"
     CDS             5074..5451
                     /gene="rpsM"
                     /locus_tag="PK077_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRN24019.1"
                     /translation="MARLFGTEIPNEKRIEASLPYIYGIGRSTSKRILEQAGINPDIR
                     TGQLTDEQLTKIVQVITTDGILIEGDLRREKQSILKRLTSINCYRGQRHRRGLPVRGQ
                     RTRTNARTRKGKKKTVGAQAKKK"
BASE COUNT         1165 a         1463 c         1748 g         1223 t
ORIGIN      
        1 gaatccccgt acgattggca gcttgtggag ctcaggcaaa gggagaatcc tgcaagaagg
       61 gcgaataaac gcatgtcttg ttgctacagt aagaagtttc gttcatcaag cccaaactcc
      121 gcatttgaaa gagagccgga cgaaaaaggc cgcggatcgg ataatccgcg gccttttttc
      181 tctattaact acctatggaa ccaaaaacca ccctgaacgg attttgaatt gctttttcca
      241 tccgtcggac agatcttgtg aaggatgaga ctccagacag gagagatgcg ttcaaaatat
      301 gttcgtcaac agttcttttt aagaggtgtt gccgtatgcg cccgagcctg tgtcccgtaa
      361 atatagcaga gattttttcc gaacatattt tgtttttcgc gggcatgtaa attttgccgc
      421 agacatttaa aatgccgggg atgtcctgac gatatattta atttttggaa taaaattatt
      481 gaaggagagg atttggcgac tgttttaaga cgtttttttg catccggatg gaaaggtttt
      541 tatacgggag atggacggcg ctcccttccg gttccatggt cagggagagg aggcccggac
      601 ctggcgtttt ccccggcgga aaagcggaat cttcctaaag ttccctgctg ttaatggaga
      661 aataccctcc agcgtccgga gtaatttgcc ctgcaatttg cattttggcc aaaagcgggg
      721 tgatagtatg cgccgctttt cccagagagg tgcagagggt gtcaatagta ttgaaaccca
      781 gtctgatggc gtggaaaatc tccttttctt ccaaagtggg aagcggtggg gttgaggctc
      841 cggaaggggg acatggcgag aagagaggca gtccctgttc cggaacggcc cagttcatgt
      901 cctgcagaat atcggaggct ccggtagcca ggatggctcc gtcccggatg agggcatggc
      961 atccgtcgga agaatgccgg tcaaccggtc cgggaatgca gaaaacgtct cggccctgtt
     1021 cggccgccgt tcgggccgtg atcagggccc cgctgcgtcc ggacgcctcc accaccagcg
     1081 tagcgcggct ccagccgctc acgatgcggt tgcgcatggg aaaagtggtg cgggaggggg
     1141 gcaggtccat cgggaactcg gagactaccg ctccatgccc gtccgcaatg cgctgcgcca
     1201 agtttctgtt ttctcgagga tacagtttgt tgagtcctgc cccgataacg gcgatggtgc
     1261 gcccctccgc gtccatggcc ccggtatggg cttccgtgtc cacgccgcgc gccagtccgg
     1321 aaatgacggt gactccggct tccgccaggt catgggagat gtttctggcg cagagcctgc
     1381 cgtaatgggt ggccatccgt gagccgacga cggcaatgga gcgttcggca tccgttccgg
     1441 tccagtttcc ccaggagtag aggacaatgg gcgggtctgg gagagcacgc agggaggatg
     1501 ggtaggagtc gtcaaaaacc gtggttaccg cggcccccgc gttatccgcc agttccagtt
     1561 ccctgtacgg gttgacgatg ctccgccaag aggaaatgcg gcaagccagc tgcgccccca
     1621 cgccgggaat ttccatcaac agggaggaag gggattccag aatcagttcc ggagaggcga
     1681 atacctgcag gagacgcatg attctgacag ggcccaggcc cggtatcagg ttcagggcta
     1741 tggctgcttc ccggggtgtc atgaataagg ggtcagttcc tggtaaacgt ccagatgggc
     1801 ttgaatcata tcctgcatat tgtaagggga aggaatttgc cggggcaggg gggggggctg
     1861 ggcatgccag cgggccagca agtccgccat ggcggcggta tctcctacgg cgacgttccc
     1921 ttcaggaagg aaggcgtcca gctgttcctt gactccgccg tgggcgtatc cggccacggg
     1981 cttgcccagg gcgagcgcct ccagggttga tttgccgaag gcttccgggc ttttggtcag
     2041 ggagagggtg acggaacatg tggaaaggat ttccctcaga tcctggcgat gtcctgtcca
     2101 ggtgaaggac tgggagacgc cggaacgttc tattgcccgc aggacctcgt ttttatattc
     2161 ttcttttccc ttctttgctt ctcctacaat gacggcgtgg gccgggattc cctgttccag
     2221 aagctgcctg acgaccggaa tcagatccag atgccctttc aggcgcgtga tgcggcccgg
     2281 caggcacagg gtgaatttcc ccttcagttc aggataggac atgaaccagc ccgtgagcca
     2341 ttcccgggag ggggagtagg ccgggtgata ttggtccggg gaaatagcat tgggtatgat
     2401 tctgatatgg tccggagggg tggacggata atggtcaagg atgtagtccc tgatgcagtt
     2461 ggagacggcg atcaccctct ctccccggct catgatggcg gagtaccggt tgacggagta
     2521 gaagccgtga acgctggtga cgaggccggg acggtcttcg ggcgggagct ttttccatgc
     2581 caggtagcct gcccacgcag gaacgcggga atggagatgc aggatatccg gcctgacggc
     2641 ctgaagcagg gcgcggaggg ccccgatgcg gaagagagtg gagatgcttt ttttgcctat
     2701 cggcatcagg atgtggcggg agccttcctt ttccagacgg ggcaccaggc gtccgcctcc
     2761 ggaaacgacg atattttcca cgccccggga agaaaggccg ctgcccagtt ccataacgac
     2821 ttgttccacg cctccggatt ccatggaggg gacgagatgt actattttca taaggcgcgg
     2881 agctgaggga aaaatctgga gaggatgtag tctgctgcac ggtccgcttc cagaagcggg
     2941 gaaccgggag ccgtcaggcg gaagttctgt tttttccagt cactgtatcc agtaaccatg
     3001 ccttcctttt ccagtgtctc caggccggac aggatgcggg atttgcggct gccgtgtcgg
     3061 cgcggtacgg aaaggatgcc tacaggcgcg ccggagccaa gagcctcata taccatggag
     3121 acgctgtcct ggctgaccca ggcggcggag gcgtgcgcca gatgccgggc tacccacccc
     3181 ggccgggttt cctccacggg aacgacggtt agttccggaa cggccgtccg gattttttcc
     3241 gcaaagccgt ccggcgtccg gcgcgaggtg gtcagaacga ggtctccggg ggtgtggatg
     3301 ctgatggacg cgagctggtt gagcatgctt tcgtcatccc agtcaaaatc tttgctgggc
     3361 cctcctatga gaatcagtgt ggtatccttt ggtacggagg tatccggcct catggggtgg
     3421 agagctccct gggtggggaa gatatccgta tccgtgtaat cgcggccgga atccaggtca
     3481 tggcggggaa taaggcagag gtcgaaaaag gagcagggaa gcgtgggctt catgcagagg
     3541 atggcccggg ttttgaaatg gtgccgcgcg cagatgagcg ggatatgcgt tgcatgtccg
     3601 gcggaaatga acagatccgg ccgtggaatg tcgctgcctg tgacgacctt ccggattttc
     3661 cccaaaaggg aaagcccctg caggttcacc gtttccacag ttccccccgc tttggagatc
     3721 agagcttgcg ccagccccag ggactggttg aggtggccct gtttcccatc gctcagaatc
     3781 cggatgttca tatatgcggc attatgaagc ggcggcgggg tgcaggcaat ccaatttcca
     3841 ccggacagaa gaaaattctt ggtgggaggc acgctcttgt tcatgattcc ggcgtgctga
     3901 ctccggattc ctctgttatt aaagattcct tgtgcgccgt gtcccggcag cttggttttt
     3961 ccggctgccg ggtggcccgt gcggaaaaaa gcccgcatgc ggaaaaattg tttcaatggc
     4021 tggagcgcgg atggcacgcc gggatggagt ggatggcgcg ttccccggag cggcgcacgg
     4081 atcctgcgga agtgcttccc ggatgccgtt ccgtcatttg cctgtcctat gattatgaca
     4141 gccccggtag gcgcccggag ggggagggta gcatttgcct ttacgcccat ggaaaggatt
     4201 accacggaat tctggaggaa aaactggcgg atttgcagga gctgctttcc atttacggcg
     4261 ggaagcagag ggggtatgtg gactccggcc ctgttatgga acgggaccat gcggaggcct
     4321 gcggcctggg ctggcgcggg aaaagcggtt tgatcgtgcg caggaaaggg ggctcccgct
     4381 tttttatcgc taccctgctg actacgctgg aactggaacc ggacgctccg gtttcaaacg
     4441 gatgcggcag ttgccgccgt tgtgcggccc tgtgtccgac gggcgcaatt atggaaaacg
     4501 gtcaggttga tgccggacgc tgcctttcct actggacgat tgaacaccgg ggggctattc
     4561 cggaagaaat ccgtcccctg ataggcacgc gcctgtacgg gtgcgatacc tgcgtgacgg
     4621 tctgtccctg gaatggtaag cctttgccgg atgttgacga acggttccgc atgtcccgtc
     4681 tgctggcttc cattcccctg cgcggtcttc tttccctgga tgccggcggt tttgcggccc
     4741 tgttccggaa ttccccgttg aaaaggctca agagggaagg gctgctgcgt aacggctgcg
     4801 ttgtgctggg gaatgcgggg actccggatg acattgattt tctgaaggcg ctttacgggg
     4861 agtctcctct ggtggaggaa cacgcttcct gggctgtgga gcgcatcctc cgccgccacg
     4921 ggcggcatgc atcttcaagc ggtgcaagag attaaaaata gaaaaaatga tgttttggtc
     4981 ttgtcaaaac ctcaatttat ggtatccatc ttgccccaag caatagctct gcgcgaggca
     5041 gagaaaaata acagctagca aatccaatcc attatggcac gccttttcgg tacagaaata
     5101 cccaacgaga agcgcatcga ggcttccctt ccgtatattt acggaattgg tcgctcgact
     5161 tctaagagaa tcctggaaca agcaggcatc aatcccgaca tccgcacggg acagcttacc
     5221 gacgaacagc tcacgaagat tgttcaagta atcaccaccg acggcatttt gattgaaggt
     5281 gaccttcgtc gtgaaaagca atccattctc aagcgtctga cctccatcaa ctgctatcgt
     5341 ggtcagcgcc accgccgcgg ccttccggta cgcggtcagc gtacccgcac gaatgcccgc
     5401 acccgtaaag gcaagaagaa gaccgttggc gcgcaggcca agaagaagta atccgccaac
     5461 ctgaattttt aatatagctt tactatggct agcgaagaaa tcaccaacga aaccgccgaa
     5521 cagcctgtgg aagcggcagc ttccgctccc gtcgctgaaa ctccggccgc cgctgtttcc
     5581 gctcctgaag aaatcaaga
//
LOCUS       DAOMSU010000133         5551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1279_length_5551_cov_2.677220, whole genome shotgun sequence.
ACCESSION   DAOMSU010000133 DAOMSU010000000
VERSION     DAOMSU010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5551
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1279_length_5551_cov_2.677220"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..866)
                     /locus_tag="PK077_06665"
     CDS             complement(309..866)
                     /locus_tag="PK077_06665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRN24020.1"
                     /translation="MKNILIISGHTNLAASVANKTILETLNERLPEAEIVKLDELYPD
                     FKIDVEAEQQKLLRADIIVLQFPLFWYSAPSILERWMEETFRHGFSHGSTGDKLKDKK
                     LILSFTTGAPETLYSREGAMGYAIDEFLPCYKAVCRLTQMEYCGSVYTCGISYGNRTT
                     PELIEQQKGASVEHAERLIRLLEAL"
     gene            complement(1063..1881)
                     /locus_tag="PK077_06670"
     CDS             complement(1063..1881)
                     /locus_tag="PK077_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HRN24021.1"
                     /translation="MNKIILFLSISFFITNAMAQEKIVQTAGRTQLGEFAPKFAELND
                     DVLFGEVWSRTDKLGLRDRSLVTITSLISQGITDNSLVFHLQSAKKNGVTRTEIAEII
                     THIGFYAGWPKAWAAFNLAKDVWAEDAAGEDAKAAFQREMIFPLGEPNTAYAKYFTGN
                     SYLAPVSREQVNISNVTFEPRCRNNWHIHKATKGGGQMLIGVAGRGWYQEEGKPAVEI
                     LPGTVIHITANVKHWHGAASDRWFSHLAIEVPGENASNEWLEPVTDEEYAKAVK"
     gene            complement(1889..2848)
                     /locus_tag="PK077_06675"
     CDS             complement(1889..2848)
                     /locus_tag="PK077_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HRN24022.1"
                     /translation="MKTISMICTLAAALMTGAGCLGQEQPKVSAEPSRVLIAYYSWGG
                     NTKYAAAQIQRATGGTLFEIKPVKPYPSEYRECTVQARKEIQEGVRPELAAKVEDMGK
                     YDVIFIGSPNWWSTIAPPVASFLASYDLSGKTVIPFVTHGGGGMARCADEVRKLCPKS
                     TVLKGGAFAGEGIRTARPALVKWVNDTITINHDKVEMENGKNTGMCDKLPMKPDGIVR
                     LSKIEVHSEYLEEYVKCAIEVGEISLRTEPGVLTMYAVAEKDNACRITILETYASQKA
                     YKSHIASAHFQKYKQETLHMVKALVLSDQTPLNPLNSITNIIK"
     gene            complement(3035..4171)
                     /locus_tag="PK077_06680"
     CDS             complement(3035..4171)
                     /locus_tag="PK077_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRN24023.1"
                     /translation="MILIFGALLAVYVFCRAILPLKLKWGWKLLLAALLAVAAFKFHL
                     LHLFGGPMFFSPVLPENVLLAAAWLFSVLFLFFFLLLAADVVRALYLLVLFCLRRNRT
                     ERFRIIGNRVNVALLVFSAVLATVGMIGGTRVPQVKEETVAVNRLPEEADGLTVAVLA
                     DLHVDGITREERIRKIVERTNALNPDIVVIAGDFVDGTVPVHGGDLRPLADLKARYGV
                     FGVPGNHEYYSGYEEWMEFLPTLGIRMLLNEHAPAGGEAVVLAGVTDPVAGIMGKEEP
                     DISKALKDAPEKGVRILVSHQPRLAREAAAHGVDLQVSGHTHGGMIAGVDRLVARFNE
                     GFVSGLYTVGNMKLYVSNGAGIWNGFPIRIGVPSEIVLIRLRKE"
     gene            complement(4262..5362)
                     /locus_tag="PK077_06685"
     CDS             complement(4262..5362)
                     /locus_tag="PK077_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRN24024.1"
                     /translation="MKITSGLALSLVSRGWAGEGSSLLPDGPGSSSGVPKKGFLGESR
                     RLGGLEVSPIGLGCLPMVGYYGGKYDKQEMIALIRRAFDKGVTFFDTAEVYGPYTSEE
                     WVGEALAPVRNQVRIGTKFGFGVEEGRPSSLNSRPDHIRRAVEGSLRRLRTDHIDLLY
                     QHRVDPDVPMEEVAGTVKELMQEGKVLHFGLSEAGARSIRRAHAECPVSAVQSEYAIW
                     WREPETKIFPTLEELGIGFVPYCPLGRAFLAGAVREDSRFQKRDRRATLPRFTPEALR
                     FNMPLAVLVREWAERRGMTPAQFALSWMLSRKPWIAPVPGTTNPAHLDDFLGGASVRL
                     SEPELKEFDLACSRIPLMGHRADPFTESQIDK"
BASE COUNT         1346 a         1611 c         1304 g         1290 t
ORIGIN      
        1 agctccgaat cctgaaaaaa caaatggaag tgttgaaata caagaaaaaa ttttatcagg
       61 atcttttaga caaccgcagg ccggacagct gcaatccgct gaactacatc agcgccagcg
      121 agccgaacat cgcgcctgag gaataaaccc tcccttaaat cctcaacaga cgtcggccgg
      181 gatttccaat gaggaatccc ggccgctctg cgttcttatt tgcttcctgc cgcctccgca
      241 tacatgaaat tcatgcagtc atcggataga gggcaattga aggccatagc gtcgaaatta
      301 ttcaaacatc agagcgcttc aagtaaccgg ataagacgtt cagcgtgttc caccgatgct
      361 cccttttgct gctcgataag ttccggtgtc gtacgattgc cgtaacttat gccgcaggta
      421 taaacactgc cgcagtattc catttgagtc agtcggcaga cagctttgta gcatggcaga
      481 aattcatcaa tcgcgtagcc catggcccct tcacggctat acagcgtttc gggtgctccc
      541 gttgtgaaag agagaatgag tttcttgtcc ttaagcttgt ctccggtact gccgtgtgaa
      601 aagccgtggc ggaaggtttc ttccatccag cgttccaata tggagggtgc ggagtaccag
      661 aagagaggga attgcaatac aatgatgtct gcccgcagca atttctgttg ttcggcttcc
      721 acgtcgatct taaaatcggg ataaagttcg tcaagcttta cgatttcagc ttccggcagc
      781 cgttcgttca aggtttcaag gattgtctta ttggctacag atgcggcaag gtttgtatgg
      841 ccgctgataa tcaatatatt tttcatatta tttcgtcgaa tacgggtctt aacctgtgct
      901 atccgagact attcctgaat acatgggcgc ctccggcaaa tcctattata ttgcgaatga
      961 aagcaacggt gccgcggaat aatcaaaata attttgtatc aaacaattac agcgccagca
     1021 cgacagcttg cgctgtaatt gtttcacatg gaagtatcca cattatttca cggccttggc
     1081 atactcctca tccgtcaccg gctcgagcca ttcattggat gcattctcac cgggaacctc
     1141 aatagccaag tgggagaacc atctgtcgga ggcagccccg tgccagtgct tcacgttggc
     1201 ggtaatatgg atgactgttc caggaagaat ttccacagca ggctttcctt cttcctgata
     1261 ccagccgcgt ccggccacac cgataagcat ctgcccgccg cctttcgtcg ctttgtgaat
     1321 atgccagtta ttgcggcaac ggggttcaaa agttacattg gagatattca cttgttcgcg
     1381 tgaaacgggt gcaagatagc tgttgcctgt gaaatacttg gcatatgccg tattcggctc
     1441 gcctaaaggg aaaatcattt cgcgttggaa agcggctttg gcgtcttcgc cagccgcatc
     1501 ttccgcccat acgtctttgg ccagattaaa tgccgcccat gctttcggcc aaccggcata
     1561 aaaaccgatg tgggtgatga tttctgcaat ctcggtacgg gtaactccat tcttctttgc
     1621 cgactgaaga tggaaaacga gcgaattgtc ggtaatgccc tgactaatga gggaggtgat
     1681 ggttaccaaa ctgcgatcac gcagaccgag cttgtcagtc cggctccata cttcaccgaa
     1741 aaggacatca tcgttaagtt ccgcaaattt gggagcaaat tctcctaact gagtgcgtcc
     1801 cgccgtctgt actatctttt cctgcgccat agcatttgtt atgaaaaaac taattgaaag
     1861 aaataaaata atcttattca ttattgtatt atttaataat attagtaata ctgtttagag
     1921 gattcagcgg cgtctggtcg gataaaacca atgccttaac catgtgtaac gtttcctgct
     1981 tgtatttctg aaaatgggcc gatgcgatgt gcgacttata tgccttttgg ctggcgtaag
     2041 tttcaagaat cgtaattctg caagcattgt ccttttcagc aacagcgtac atggtcaaca
     2101 cgcccggttc cgtgcgcagg gagatttccc ccacttcgat ggcgcatttc acatattcct
     2161 ccaaatattc agaatgcact tctatttttg agagacggac gataccatca ggcttcatcg
     2221 gtaacttgtc gcacatgccc gtatttttgc cattctccat ttccacttta tcgtgattta
     2281 tggtgatcgt gtcattgacc cacttgacca gcgcgggcct ggccgtccgg attccttccc
     2341 cggcaaaagc gcctcccttg agaacggtgg acttgggaca cagcttccga acctcgtccg
     2401 cacagcgcgc catgccgccg cccccatgcg tcacaaatgg aatcaccgtc ttgccggaaa
     2461 ggtcgtaact tgccaggaaa gacgcgaccg gaggcgcaat ggtgctccac cagttggggc
     2521 tgccgatgaa aatcacatca tacttgccca tatcctctac tttggcggcc agttccggcc
     2581 gaaccccttc ctgaatctcc ttcctggcct gcacggtgca ttcccggtat tcggagggat
     2641 aaggcttgac cggcttgatt tcaaacagcg ttcctcccgt cgccctctga atttgcgcag
     2701 cggcgtactt cgtattgcct ccccatgaat aataggcaat gaggaccctg gaaggttccg
     2761 cggaaacctt gggttgttcc tgtcccaaac atccggctcc tgtcatcagg gccgcggcca
     2821 gcgtgcatat cattgatatt gttttcatgt atttttatcc ggttaaaagg agaggcaaaa
     2881 ggcgtccaga aaaaacggtt tcatccggca tttcccttgc ctgaccttgt tccttatttc
     2941 tgtctaaaca ttagagtaac tataagtcaa tattttttca acaattccgg caatcctgcc
     3001 cgcatcactt cctaaactgc tttatcaata tatgttattc tttccgaagc cgaatcagaa
     3061 caatttccga tggaacgccg atgcggatgg gaaagccgtt ccaaatcccc gccccattgg
     3121 aaacgtacag cttcatatta cccaccgtgt acagcccgga gacgaatccc tcgttaaaac
     3181 gcgccaccag ccggtccacg ccggcaatca tgcctccgtg cgtatggccg gacacttgaa
     3241 gatccacgcc gtgcgcagcc gcttcccggg ccagccgcgg ctggtgggaa acaaggatgc
     3301 gcactccctt ttccggggca tccttcaacg ccttgcttat atccggctcc tccttcccca
     3361 tgatgccggc cacaggatcc gtcacaccgg ccaataccac ggcctcccct cccgccggcg
     3421 cgtgttcatt aagaagcata cgaatgccca gagtgggcag gaactccatc cactcctcat
     3481 aaccggagta atactcatga ttgcccggca cgccgaacac cccgtatctg gccttcaaat
     3541 ccgcaagcgg cctcaaatcg ccgccatgca cgggcaccgt cccgtccacg aaatctccgg
     3601 caatcacgac gatgtccgga ttcagggcgt tcgtgcgctc cacaatcttc cgaatgcgct
     3661 cctcccttgt aatgccgtcc acatgcagat ccgccagcac ggcgaccgtc aatccgtccg
     3721 cctcctccgg caggcggttc acggcaaccg tttcctcctt cacctggggc actctggtgc
     3781 ctccaatcat gcccacagtt gccagcacgg cggaaaaaac cagcaaagca acattaaccc
     3841 ggttgccaat gatgcgaaac ctttccgtcc tgttcctccg caagcagaac aaaaccagca
     3901 gatacagagc ccgcaccaca tccgccgcca gcagcaggaa aaagaacagg aaaagaacgg
     3961 agaacagcca cgcggccgcc agcagaacgt tttccggcaa aaccggggag aaaaacatgg
     4021 ggcctccaaa caaatgcagc aagtggaatt tgaatgccgc caccgccagc agggcggcaa
     4081 gcagaagctt ccagccccat ttcagcttca gcggtaaaat agcgcggcaa aaaacgtaaa
     4141 ccgccagcaa tgctccgaaa atcagaatca tatgtctctt tcttaaaaat agcttaacat
     4201 ccttccgccg caaacagtcc cgcggcattt ttccggagcg gaaggaaacg cgccggcaaa
     4261 actacttgtc aatctggctc tccgtaaacg gatccgcccg gtgccccatc aggggaattc
     4321 tggaacaggc aaggtcgaat tccttgagtt ccggttcgga caagcggacg gaagcccctc
     4381 ccagaaaatc atccagatgg gctggattgg ttgttccggg aacaggcgca atccacggtt
     4441 tccgggaaag catccaggac agggcgaact gggccggagt catgcccctg cgttccgccc
     4501 attcccggac aagaacagcc agcggcatgt tgaatctgag ggcttccgga gtaaaccggg
     4561 gcaaagtggc gcggcggtcc cgcttttgaa aacggctgtc ctcccggacg gctcctgcca
     4621 gaaaggcgcg ccccagcgga caatacggaa caaaaccgat gcctaactct tccaacgtgg
     4681 gaaaaatctt cgtctccggt tccctccacc agatagcgta ttcgctctgg acggcgctca
     4741 ccggacactc ggcatgagcc ctcctgatgg aacgggcgcc ggcttcggac aggccgaaat
     4801 gcagcacttt tccctcctgc atcagttcct tcaccgtacc tgccacctcc tccatcggaa
     4861 catccgggtc cacccggtgc tggtaaagca ggtcaatgtg gtcggtacgc aaacgcctga
     4921 gggaaccttc taccgcacgc cggatatggt cgggcctgct gttcagggaa gaaggacggc
     4981 cttcctccac gccaaaaccg aatttggttc ctatcctgac ctggttgcgg acaggggcga
     5041 gagcctcccc cacccattcc tcactggtat aaggcccgta cacttccgcc gtatcaaaaa
     5101 aagtaacccc tttgtcaaaa gcccggcgta tcagggcaat catctcctgt ttatcatact
     5161 tgccgccgta ataacccacc atcggcagac atcccagccc gataggggaa acttccaggc
     5221 ctccaaggcg ccgggattcc ccaagaaaac ccttcttcgg caccccggaa gaagaccccg
     5281 gaccgtcagg cagcaaagaa gaaccctccc cggcccatcc ccgggaaacc agagataagg
     5341 ccaaaccgga tgttatcttc aaaaaatcct tgcgtttcat tacgttccgt ctttctttcc
     5401 caaattcaga tgcggggcct ccttatttca gatgctccag aaagaactgt ccgattctgt
     5461 cgaaaggaat gacaccggct tgattgtcat aaagatccac atggcttgca ccgggaataa
     5521 tcatcagctc cttgttgtcc cccttcaact t
//
LOCUS       DAOMSU010000134         5515 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1293_length_5515_cov_2.821795, whole genome shotgun sequence.
ACCESSION   DAOMSU010000134 DAOMSU010000000
VERSION     DAOMSU010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1293_length_5515_cov_2.821795"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK077_06690"
     CDS             complement(<1..506)
                     /locus_tag="PK077_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24025.1"
                     /translation="MLLLRLPAVFLPLFISFLFGACSKKESALLPGEIIVNASELPGP
                     IQEWKTDLACERCLTINLDNPSLPWFRIAEGISEAYAHGVNNLRLKRGDAIASAFGNG
                     ICWDLPVSTDMAPESDPESEKGMNPDIPCLPDDAVLEHPFREDDFLVFNKTPSRKPVY
                     PIVKFIRSP"
     gene            653..1210
                     /locus_tag="PK077_06695"
     CDS             653..1210
                     /locus_tag="PK077_06695"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRN24026.1"
                     /translation="MSVITKRGDSGETDLMFGRRSPKTAPRIEAYGTVDELNSLIGVV
                     RHSGVSSRTVEMLDGVQARLVGAMGELATMEEDLPKYDAKGYARITAEDVAWLEEQAH
                     LLEKECDIRFKGWARPGKEGSLGSAYLDLARSVCRRAERRVVALRLDHALSNVNTALF
                     LNRLSDLCWVLARFEALAAGEKIQA"
     gene            complement(1370..1708)
                     /locus_tag="PK077_06700"
     CDS             complement(1370..1708)
                     /locus_tag="PK077_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRN24027.1"
                     /translation="MTAIQRLPERTPFNYADLTDYREGQITSLALIRSKGVNMTILAF
                     DNEQLLPTHQTPGPALIQILDGTAYFTVGNEEVILPQGKSLLIPAGVPHSVIARERFK
                     MLVTSILPLD"
     gene            complement(2013..4283)
                     /locus_tag="PK077_06705"
     CDS             complement(2013..4283)
                     /locus_tag="PK077_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HRN24028.1"
                     /translation="MNHSLKDRLIRHMEDGRYEPQSKSELARALNVDSRQKLDFRALV
                     DQMEEEGKLVRLQKGRYALKRERRNLVHGMIRILRSGKILFLPRKGDPAAAALGWDTE
                     AIPELELKPNRLGTALDGDRVAVRVERKAARGRRNIRRDRFSSPDAGMKARVEEVTER
                     ARSRWLGVFRTGKNKPGRVLGDGVSSPSSIELAEKPAMEVLPGQLVSVEPVTCGEEKK
                     APRGKIVEVLGYPDEPHVDMEAVIRKYGLSAEFPASVLRELETLPQNPSPGELARREN
                     WTDRTVITIDPASARDFDDAISITATSSGWTLAVHIADVSHFVRPGGALDGEALRRGN
                     STYLPDRVLPMLPPCLSDDLCSLRPDVVRLTKVCEMKFDQKGKMLRARFADAFIRSKA
                     RLTYQEAFAMLKGNDKGEVPSTVREAWNLASILRRNRYAKGALDLDFPEVRAVMDKDG
                     RVTGIITEEYDESHQLIEECMLAANEAVALALKNGNRPTIYRVHEEPDSSKLFEFGQL
                     CKLYGYPVHDIGQRQYLNELMKSIKGSPDEQLLKLALLKSLMRARYDTEPLGHYGLAT
                     PNYCHFTSPIRRYADLVVHRSLNPLLANPPKGAKGAGSAGRLEEDAEHISETERISAS
                     AEKDANRMKLFEWLEGQCYTEHPEVHEALVTETRHFGVLLEIPRLQIKGLVKPDKLPG
                     GRWVYEAFASRWKNDHGSVLCAGLRVPVIPVKVDREQQWADFAIVSREKPRQTGKTAF
                     PAKQEKGTSGHGRRNR"
     gene            4519..5469
                     /locus_tag="PK077_06710"
     CDS             4519..5469
                     /locus_tag="PK077_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24029.1"
                     /translation="MKTFIFPFLACCFLAFNALASKGVEIRHKTQFCAAEVYFRYPDR
                     NPPQEEIDKSNRERTTVTLREYITLTATLDGKELPEDTRCDWSAVEGGEYFKLEHSNE
                     EFGTVTVNINTLAKARQTLKVKAEVDGVEQVVEFTLIPPQGLMYAGAESPFRQAEEEA
                     AKVGCTVGLSALRILRVYPLNVSFTNLYSLEQHAGYEPDPLPDHPLVSEHAPLPEPAR
                     VSVTNEVYDSTNCLYPPALIDAFSGSIGWTWKCRWQIFGCTDKPQGGRYTKEKVLWDF
                     NTDQVFSIRRDPDTGTVHYRIQKFKGLNKNDPGIVTATSN"
BASE COUNT         1116 a         1497 c         1655 g         1247 t
ORIGIN      
        1 ggggaccgga tgaatttcac gatgggataa acgggtttcc gggaaggtgt tttgttgaaa
       61 acaagaaagt catcttcacg gaaaggatgt tccagaacgg catcgtcggg aaggcaggga
      121 atgtccgggt tcatgccttt ttcagattct ggatcagatt ccggggccat atccgtggag
      181 acgggaagat cccagcaaat tccatttcca aaggcggacg cgatggcatc ccctcttttc
      241 aaacggagat tattcacgcc atgcgcataa gcctccgata tgccctcagc gatgcggaac
      301 cagggaaggg acggattgtc cagattgatg gtcaggcatc tttcacaggc caaatccgtc
      361 ttccattcct gtatggggcc gggcagttcc gatgcgttca cgattatttc tccgggcagc
      421 aaggcgcttt cctttttaga gcatgccccg aagaggaagc tgatgaagag agggaggaaa
      481 acggcaggaa gacgaagcag aagcatttat tcattcttct tatatagttt ttattgcatg
      541 tcaaaagaat agagagtgcg gtacatgccg ggaaagatgg cgctggctcc cgctccggaa
      601 tatgccttct tttttgcgtg caggacggcg cgggcatggt agggtacatg gtatgagcgt
      661 aatcaccaag cgtggagaca gcggagaaac ggacctgatg ttcggcaggc ggagtcccaa
      721 gacagcgccg cgcattgaag cttacggaac ggtggatgaa ttaaattccc tgattggcgt
      781 ggtgcgccat tccggggtaa gctcccggac ggtggagatg ctggacggcg tccaggctcg
      841 cctggtgggt gccatggggg aactggccac gatggaggag gatttgccca aatatgacgc
      901 caaggggtac gcccgcatta cggcggaaga tgtggcatgg ctggaggaac aggcccattt
      961 gctggaaaag gaatgcgaca tccgtttcaa aggctgggcc cgcccgggca aggaaggatc
     1021 gctgggctcc gcgtatttgg acctggcccg ttccgtctgc cgccgtgcgg aacgccgtgt
     1081 ggtggcattg aggctggatc atgccctgag caacgtgaac acggccctgt tcctgaaccg
     1141 cctttccgat ctctgctggg tgctggcccg ttttgaggcg ctggccgcag gggaaaagat
     1201 ccaggcctga gcaggccgtt tccgcccgtc ctcctgttcc ggcatgttgc gtatctgcgc
     1261 gattcccgtg tcaggagaag cctggaaaga atggttttgc cgcagatcca tgtccaggtg
     1321 gcaattcccc ggaatggttc ttcgttattt tcaggggagg gcttcccgat cagtccagcg
     1381 gcaggatgga agtaaccagc attttgaacc gttcccgggc aatgacggaa tgaggcactc
     1441 cggcgggaat caggaggctc tttccctggg gcagaataac ttcttcattg cccacggtga
     1501 agtacgccgt cccgtccaga atctgtatca gtgcaggacc gggcgtctgg tgagtgggaa
     1561 ggagctgttc gttgtcaaag gccagaatgg tcatgttgac tcctttggag cgaataaggg
     1621 ccagactggt gatttgccct tcgcggtaat cggtcagatc ggcgtaatta aagggagtgc
     1681 gttcggggag gcgctgaatg gctgtcatgt aaattggaca cccttgttgg cccttcccgt
     1741 ttcttcatga caggcttccg ggaaaaaggt ccgctccaga cccgggagcg gaccttggca
     1801 ttcagcattc atatggtgct tacttacgga gtcagcgtcc tgaattcata ggcgtgaggt
     1861 cggattcaca agtaaacaac agttattcta tacatgaaaa atacaattta cttcaagaaa
     1921 aattgtgtag gataatgcag atttgattct gctgcaggga gaatcggatt tgactcccgt
     1981 atgggattgt ttccatatac gggcgtatgc tgtcagcggt tccgccgccc gtggccggat
     2041 gtgcccttct cctgtttggc ggggaaggcc gtttttcccg tttgccgcgg tttctcccgt
     2101 gagacgatgg cgaaatccgc ccactgctgc tccctgtcca ctttcacggg aatgacggga
     2161 acgcgcagcc cagcgcacag cacggaacca tggtcgttct tccagcggct ggcgaacgcc
     2221 tcatacaccc agcggccgcc gggaagcttg tccggcttga ccagcccctt aatctggagg
     2281 cgcggtattt ccagcagcac gccgaagtgg cgcgtttccg taaccagagc ttcgtgcact
     2341 tccggatgct ccgtgtagca ctggccttcc agccattcga agagtttcat ccggttcgcg
     2401 tccttttccg cgctggcgga aatgcgttcc gtctctgaaa tgtgttccgc gtcttcctcc
     2461 agcctgcctg cactgcctgc tcctttggct cctttgggag gattagccag caggggattc
     2521 agggagcggt gaaccaccag gtccgcgtaa cggcggatgg ggctggtaaa atggcagtaa
     2581 ttgggagtgg ccaggccgta gtggcccagg ggctccgtgt cgtacctggc cctcatcagg
     2641 cttttcagga gcgccagctt aagcaattgc tcgtccgggg aacccttgat ggacttcatc
     2701 agttcattca ggtactggcg ttgtccgata tcgtggacgg gatacccgta caatttgcac
     2761 aactggccga attcaaacag tttggaggaa tccggctctt cgtggacacg gtagatggtg
     2821 ggacggttcc cgtttttcag ggccagggcc acggcttcgt tggcggccag catgcactcc
     2881 tcaataagct ggtggctttc atcgtactct tccgtgatga tgcccgtcac gcggccgtcc
     2941 ttgtccataa cggctctcac ttccgggaag tccagatcca gggctccctt ggcgtagcgg
     3001 ttgcggcgca ggatggaggc cagattccag gcttcccgca ccgtggaggg aacttccccc
     3061 ttgtcgtttc ccttcagcat ggcgaaggcc tcctgatagg tcaggcgcgc cttgctgcgg
     3121 atgaaagcgt ccgcaaagcg tgcacggagc atcttcccct tttgatcaaa tttcatttcg
     3181 catacttttg tcaggcgcac gacatccggc cgcaggctgc acaaatcgtc ggaaaggcag
     3241 ggcgggagca ttggaaggac gcggtccggc aggtacgtgg aattgccgcg gcgcagggct
     3301 tccccatcca acgcgccgcc gggcctgacg aaatgggaga catccgcaat gtgaacggcc
     3361 agcgtccagc cggacgaagt ggccgtgatg gaaatggcgt cgtcaaaatc ccgggcgctt
     3421 gccgggtcaa tggtgatgac ggtacggtcc gtccagtttt cccggcgggc gagttccccg
     3481 ggggaagggt tttgaggcag cgtttccagt tcccggagca cggaggcggg aaattccgcg
     3541 gaaaggccat attttctgat aacggcttcc atgtccacgt ggggctcgtc cggatagccg
     3601 agcacctcca cgattttccc gcgcggggct ttcttttcct caccgcaggt tacgggttcc
     3661 acggaaacca attggccggg aagcacctcc atggcgggtt tttccgccag ttcaatggat
     3721 gacggagagc ttacgccgtc tcccagcacg cggcccggct tgttctttcc ggtgcggaag
     3781 acgccaagcc agcgggagcg cgcgcgttcc gtcacttcct ccacgcgggc tttcatgccg
     3841 gcatccgggg aagaaaagcg gtccctgcgg atattcctcc ggcccctggc cgccttgcgt
     3901 tccacgcgca cggccacccg gtccccgtcc agggcagtac ccagacggtt gggtttaagt
     3961 tccagctccg gaatggcttc cgtgtcccat cccagggccg cagcggcggg atcccctttt
     4021 ctggggagaa aaagaatctt gccggagcgc aggatgcgga tcatgccatg caccaggttc
     4081 cggcgttccc gctttaaagc gtatctgccc ttttgtagcc gcacgagttt tccttcctcc
     4141 tccatctggt ccaccagggc gcggaaatcc agcttctgcc gggaatccac gttcagggcg
     4201 cgggccagtt cggatttgct ctggggttcg taacgtccgt cttccatgtg acggatcagg
     4261 cggtctttaa gggaatgatt cattttgcgg aagttccggg aaaaggtgtc tcccattccc
     4321 atggaaacca tcatagggcg gtttgcgcgc cgcttcaagg cgcgggcggt gcatgccgcg
     4381 ttcgtcagcg gtttttggcc tgctgccggg catgccgttg tacgggaccg cagtccgtct
     4441 aggaattttt ttaattatgt ggcatatgga ttgacaatga tctgtttttt ggatttaacg
     4501 atgtttggtc gtttttttat gaaaacattt attttcccgt ttttggcctg ctgttttctg
     4561 gcgttcaacg cgctggcgtc caagggcgtt gaaatacggc ataaaaccca gttctgcgcg
     4621 gcggaagtat atttcaggta tcccgatcgc aaccctcccc aggaggagat agacaaaagc
     4681 aacagagaaa gaacgaccgt caccctccgg gaatacatca ccctgaccgc cacgctggac
     4741 ggcaaagagc ttcccgaaga cacccggtgt gactggtccg ccgtggaagg aggggaatat
     4801 ttcaagcttg agcatagtaa tgaagaattt ggtacagtaa ccgtcaacat caataccctg
     4861 gccaaagcgc ggcagacgct caaggtgaaa gctgaggtgg acggggtgga gcaggtggtg
     4921 gaattcaccc tcatcccgcc ccaggggctg atgtacgcgg gggcggagag ccctttccgg
     4981 caggcggagg aagaagcagc caaggtggga tgcaccgtgg ggctgagcgc cctccgcatc
     5041 ctgcgcgtgt atcccttgaa cgtcagcttc accaatcttt actccctgga gcagcatgcc
     5101 ggttatgagc ccgaccctct tccggaccat cccctggtca gcgagcatgc gcctttgccg
     5161 gagcccgcac gggtttccgt caccaatgaa gtctatgaca gcaccaactg cctgtatccg
     5221 ccggcgctca ttgacgcatt ctcggggtcc atcggctgga cgtggaagtg ccgctggcag
     5281 atattcggat gcacggataa accccaaggc ggcaggtaca cgaaagaaaa agtgctctgg
     5341 gatttcaaca cggatcaggt tttctccatc aggcgcgatc ccgatacggg aacggtgcac
     5401 taccggatcc agaagttcaa gggattgaac aaaaatgacc cgggcatcgt caccgcaaca
     5461 agcaactgat tttctttatg aactattacc tttccctctg tctttccgtg tttct
//
LOCUS       DAOMSU010000135         5487 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1304_length_5487_cov_2.832842, whole genome shotgun sequence.
ACCESSION   DAOMSU010000135 DAOMSU010000000
VERSION     DAOMSU010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1304_length_5487_cov_2.832842"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            67..699
                     /locus_tag="PK077_06715"
     CDS             67..699
                     /locus_tag="PK077_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN24030.1"
                     /translation="MKLLCESVCFSYSGKQWIFEDYNRVFTSGITILKGYSGCGKTTL
                     LKILAGYLKPQSGRVVTPRRKVPADSLYRRKEVSYMFQGLNLLPLATVERNLQLCAEM
                     AMLPKKQWTRRADDLITRLGLESLRHKKAGSLSGGQAQRAALARTMMKDSDVLLLDEP
                     TSGLDDGNTEIIKNLIREYPADKICILSTHDSRLFDLTHEIMDFNLSVSL"
     gene            662..1807
                     /locus_tag="PK077_06720"
     CDS             662..1807
                     /locus_tag="PK077_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN24031.1"
                     /translation="MKSWILTCLYLFKDTLHRWKERPASPLSRILVAFFLSLCALSFL
                     ANYVLSAKMLHDEIRKNGADLIIVFDTVRDGEPSKKNLLQHELPSLYGCDVLVLNDAR
                     AGVARVGKTVFPILEYNMESCSFLSALGLEDVATVLLYNPSRSPLQAGPCTVTIADFD
                     FSLNASPLPEGHLLGRLYPSGVILAPEGTFSSGQEVPVENCRYIIRVHEMTAARIRDI
                     EEVLNNMVRYDGSMTMVQTHVGLLDRLEVLMGNQTECRTGFSLGIAVIVGILLTALAS
                     MEFRQNEYVYTLMKSFGVRPVLLIMAFIVENAFLIGVSFAGAVWAFMKTQRIVLGEFF
                     KLGNSVLTYADIRQDLWLLGISLAGCVLLSSIPVAFSACRPIGKVLK"
     gene            complement(1979..2455)
                     /locus_tag="PK077_06725"
     CDS             complement(1979..2455)
                     /locus_tag="PK077_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24032.1"
                     /translation="MNSRLYIPALITLGLGILSGCGGSSSSEQAYLPTGLQRTLDITL
                     QDGKPYVFSFIIASESTATIYTGTSPAASTASLHVVNYKPSSDSAEQIGFYWSSNDGE
                     TTNNLDALVVLYNPQNISKGIGSATCSVRDAEYTETETQQDNCQAVIRDFRVSSSS"
     gene            complement(2484..2999)
                     /locus_tag="PK077_06730"
     CDS             complement(2484..2999)
                     /locus_tag="PK077_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24033.1"
                     /translation="MMRPFFTSLSAACCSLGILTLAQCGGSSSDAEGLAPSTIGSKVL
                     NMQVTGITDAAITLPAIFDMTTGDNGAFIIQTQGEDMNMWNGAGIYSYTKTGKNTAEL
                     ILSYNRNSGSSSEPNTLMSTIRIPQLTFENATHASSGEGGASISYMQAQDWTPIPIDG
                     ITISVNIISQS"
     gene            3199..4488
                     /locus_tag="PK077_06735"
     CDS             3199..4488
                     /locus_tag="PK077_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24034.1"
                     /translation="MFPFPPVVRSFLFSLFERAWLRTALCFCLCATVCASCSVDRHME
                     KKAGELMARMDAVPDWRQLPRKEISWHQALAMMMERNIDLKKSEQSLKTTKRSVINVF
                     TQIIPGVNLDWMLTKELSDLAKVTASDVEYNTNILFNMPSLTQIPFDYYSAKAAVYTA
                     EKTLEMKKRELVARLYQQVLSYRNAQISYNNQLSSLPYDDDGVQKKKLDLERERNLNE
                     ISQGFAVLLGNMDARWLVKPETMPRLDWGRYRAASRQLDLLVVTMVAMELEASRLQVL
                     NAKLKFFPSVDINFYSPTLFSSTGGTYGGFFAGAGDMKVNMSLREELDTRLTSWFQYK
                     SAKESHELLQREVVMELQRRRIKIAALLESRRRFELWQGVLMKEIAFKESRLSVSGDE
                     YLEQRKDIRKMYADLDNEASKNAEVEAALIMEYGWLK"
     gene            4599..>5487
                     /locus_tag="PK077_06740"
     CDS             4599..>5487
                     /locus_tag="PK077_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24035.1"
                     /translation="MALMILAFPAAIYGAFPWGIALGAFLFLLGVVVAASRFSAIVWS
                     WCLFSLGMYLAAPFIWMWNPDYFFSRSFDILLISGCLVGSLVLLAIRFAVIPVLWPRI
                     FRHFVEGSPTLEDLAPEKGKTVAELRGGIERRQVNFLFGHADRELVEKWGKIAQGIAA
                     LSRSRKRLAIWAAGIFLAWPAAVFFLSGGMGGALDRDIDRMWGTRPFRDGMVVSSPAA
                     MNAARRVFRDARALRGLTMEQARAWLRTDRRSAAFKLGKPRYDFEKDETVLVLSDGRD
                     SIRLVAVFNDAGQITHSWPE"
BASE COUNT         1156 a         1280 c         1703 g         1348 t
ORIGIN      
        1 tgaaaaagtg catccggacc tggagctggt ggtgaatgga gaaacggaat tgattgttcg
       61 aaaaaaatga aattgctgtg cgaatcagtc tgtttcagtt attcaggcaa gcagtggatt
      121 tttgaagatt ataacagggt attcacttcc ggaatcacca ttttgaaagg gtattccgga
      181 tgcgggaaaa cgactctgtt gaaaattctg gcggggtatc tgaagccgca gagcgggcgc
      241 gtggttactc cccgccggaa ggtcccggcg gattccctct accggcgcaa ggaggtgagc
      301 tacatgttcc aggggcttaa tctccttccc ctggctacgg tggagagaaa ccttcaattg
      361 tgcgcggaaa tggccatgct gcctaaaaag cagtggacgc ggagggcgga tgatttgatt
      421 acccgcctgg gcctggaatc tctgaggcat aaaaaggcgg ggagcttgtc cggcggccag
      481 gcccagcggg cggctcttgc ccgcacgatg atgaaggatt ccgatgtgct gctgctggac
      541 gaacctactt ccggcctgga tgacggcaat acggaaatca ttaaaaattt gatcagggag
      601 taccctgctg acaaaatttg cattttgagc acgcatgatt cccggctgtt tgacctgact
      661 catgaaatca tggattttaa cctgtctgta tctctttaaa gatactctcc accgttggaa
      721 ggaacgcccg gccagccccc tttcacggat tctggtggcc ttcttccttt ccctgtgcgc
      781 gctctctttt cttgccaatt atgtcctctc cgccaaaatg ctgcatgatg aaatcaggaa
      841 gaacggggcg gacctgatta ttgtttttga taccgtgcgc gacggtgagc cttccaagaa
      901 aaatttgctg cagcatgaac tgccttccct gtatggctgc gacgtcctgg tgctgaatga
      961 tgcccgtgcg ggcgtggcgc gcgtgggcaa gacagtattc cccatcctgg aatacaacat
     1021 ggagtcctgt tcctttcttt ccgcgcttgg gttggaggac gtggctaccg tcctcctgta
     1081 caatccctcc cggagcccgt tgcaggcggg gccttgtacg gttaccatag cggattttga
     1141 tttttccctg aatgcctccc ctctgccgga ggggcatttg ctgggcaggc tgtatccttc
     1201 cggagtcatt ttggccccgg aaggaacctt cagttccgga caggaggttc ccgtggaaaa
     1261 ttgccgctac atcatccggg tgcatgaaat gacggcggcc cgcattcggg acatagaaga
     1321 agttctgaac aacatggtgc gttatgacgg cagcatgacc atggttcaga ctcacgtagg
     1381 gctgcttgac aggctggaag tgctgatggg caaccagacg gagtgcagga cgggattttc
     1441 cctcggtatc gcggtgatcg tcggcattct gcttacagcc ttggcatcca tggaattccg
     1501 tcagaatgaa tatgtctata cgctgatgaa gagcttcggg gtgcgtcctg tactgctgat
     1561 tatggcgttt atcgtggaaa acgcatttct gattggcgtt tcctttgcgg gagccgtctg
     1621 ggcgtttatg aaaacccaga gaatcgtttt gggggaattt tttaagctgg gaaactccgt
     1681 cctgacttat gccgatatac ggcaggattt gtggctgctg ggaatttccc tggcgggatg
     1741 tgttctgctt tcctccattc cggttgcctt ttccgcctgc cgccccatag ggaaagtgct
     1801 caagtaagtg atggagcttc gggggctgct cccggttttg tctgcgttcc ggcatgtccg
     1861 cctttgccgg cggtctggga gggaaagtcc gtctctgttc cggcaggaga gatcagggta
     1921 aaaaaatccc ttcccgccat gggagcagga agggaggtat gaagaaaggg cttattgatc
     1981 aagagctgga ggatactctg aaatccctga tgaccgcctg gcagttatcc tgctgggttt
     2041 ctgtttcagt atattcggcg tctctgacgc tgcaggtggc agaaccgatg cctttggaga
     2101 tattctgggg attatacagg acgacgaggg catccagatt gttggttgtt tccccgtcat
     2161 tggacgacca gtagaagcct atctgttccg cagagtcgga ggaaggcttg tagttgacga
     2221 catgaaggga tgccgttgag gcggccgggc tggttccggt gtaaatggtg gcggtgcttt
     2281 ctgaggcgat gatgaaggag aagacgtaag gcttgccgtc ctgaagcgta atgtccaacg
     2341 tgcgctgaag gccggtggga agataggcct gttcgctgga actggatccg ccgcagccgg
     2401 agaggatccc aagcccgaga gtaattaaag cgggaatgta aaggcgggaa ttcatgattt
     2461 gatggataaa ggaaagaatt tatttatgac tgggatataa tgttgacgct gatggtgatg
     2521 ccgtcgatgg ggatgggcgt ccagtcttgc gcttgcatgt aggaaatgct ggcgccgcct
     2581 tccccggaag aagcatgggt ggcgttctca aaagttaact ggggaatcct gatggtgctc
     2641 atgagggtgt tgggttccga ggatgaaccg ctgtttctgt tgtaggacag gatcagctcc
     2701 gcggtgtttt tcccggtttt ggtgtaggaa taaatgcctg ccccgttcca catgttcatg
     2761 tcttcccctt gcgtctggat gatgaacgcc ccgttgtcac cggtggtcat gtcaaaaatg
     2821 gcaggaagcg ttatggcggc atccgtaatg cctgttacct gcatgtttaa cactttgctg
     2881 ccgatggtgg agggcgccag gccttcggca tcggaactgc ttccgccgca ttgggccagc
     2941 gtaagaatgc cgagggagca gcaggccgcg gacaaagagg taaaaaaagg acgcatcata
     3001 aaaggataat aattttccga taaactgtga tatcacacat gatttgtcaa gataggaatc
     3061 aggagggagg tattttttac acggggaaag gaaaaaccga attccctgcg ctccaggact
     3121 tcttttttcc ttctttctcc gcctgccgtg ttgatgcttg acttttcagg ggcagaagct
     3181 atgtaacaag gggcctgtat gttcccgttt cctcctgttg tccgttcttt cctgttttcc
     3241 ctgtttgaaa gggcatggct gaggactgcg ttgtgcttct gcctctgtgc gacggtatgc
     3301 gcctcctgct ccgtggacag gcacatggaa aaaaaggcgg gggaattgat ggccaggatg
     3361 gatgccgttc ccgattggcg gcagctgccg cggaaggaaa tttcatggca ccaggccctg
     3421 gcgatgatga tggagcggaa catcgacttg aaaaaatcgg agcagtccct caagacgacg
     3481 aaacgttccg tcatcaatgt ttttacccag ataatccccg gcgtcaatct ggattggatg
     3541 ctgaccaagg aattgagcga cctggccaag gtgacggcca gcgatgtgga atacaacacg
     3601 aacatcctgt tcaacatgcc gtccctcacc cagatcccgt tcgattatta ttccgccaag
     3661 gcggctgttt atacggcgga aaagacgctg gagatgaaaa aaagggagct ggtggccaga
     3721 ttgtaccagc aggtgctttc ctaccggaat gcgcagatca gctacaataa ccagctgagc
     3781 tccctgcctt atgacgatga tggcgtccag aaaaagaagc tggacctgga acgggaacgg
     3841 aatttgaatg agatttccca gggttttgcc gtgctgctgg ggaatatgga cgcccggtgg
     3901 ctggtgaaac ctgaaacgat gcccaggctg gactggggca ggtacagggc ggcgtcccgc
     3961 cagttggatc tgctggtggt gacgatggtg gccatggagc tggaagcttc ccgcctccag
     4021 gtgttgaatg ccaaattgaa gttcttcccg tccgtggata ttaattttta cagcccaacc
     4081 ctgttttcca gcacgggcgg gacgtacggc ggtttttttg cgggagcggg cgatatgaag
     4141 gtgaacatga gcctgcggga agagctggat acgcgcctga catcctggtt ccagtataag
     4201 tcggccaaag aaagccacga actgctgcag cgcgaagtgg tgatggaact gcagcggcgg
     4261 cgcatcaaga tagccgcgtt gctggagagc cgcaggaggt ttgagctctg gcagggggtg
     4321 ctgatgaagg aaattgcgtt caaggagtcc agattatccg tttccggaga tgaatatctg
     4381 gaacagagga aggatatcag gaaaatgtat gctgacctgg ataatgaggc ttccaagaat
     4441 gcggaggtgg aagcggccct catcatggaa tatggctggc tgaaataagg cgggggagga
     4501 tgaacaggcc ctgtgtgact gcgggggctt cctttcttga tgctttcaag ggcttgtgct
     4561 atgttccgga tatgcaaaaa gttcggcagc ccgcgggtat ggcattgatg attctggctt
     4621 ttccggccgc catttacggg gcgtttccgt ggggaattgc cctgggggcc tttttgttcc
     4681 tgctgggggt ggttgtggcg gccagccgtt tttccgccat agtctggtca tggtgcctgt
     4741 tttccctggg gatgtatctg gcggctcctt ttatctggat gtggaatccg gactattttt
     4801 tcagccgttc gtttgacatt ctgttgataa gcggctgcct ggtgggcagc ctggttttgc
     4861 ttgcaatccg gtttgcggtt attcccgtgc tgtggcccag gattttccgc cacttcgtgg
     4921 aaggaagccc cacgctggag gatctggcgc ccgaaaaggg aaagacggtg gctgaactgc
     4981 ggggcggcat agaacgcagg caggtgaatt tcctgttcgg gcatgcggac cgggaactgg
     5041 tggaaaaatg ggggaaaata gcccagggaa tagcggctct ttccagaagc cgcaaacggc
     5101 tggcaatatg ggctgcgggt attttcctgg cttggcccgc agccgtgttt ttcctttccg
     5161 gggggatggg cggcgcgctg gaccgggata ttgaccgcat gtggggaacc cgcccgttcc
     5221 gggacggcat ggtggtttcc agccctgcgg cgatgaatgc ggcgcggcgc gttttcaggg
     5281 atgcccgggc gctgcgtggc ctgacgatgg agcaggcgcg cgcgtggctg aggacggacc
     5341 gcaggagtgc ggcattcaaa ttgggcaagc cccgttatga ttttgaaaag gatgaaaccg
     5401 tgctggtgct ttcagatggg agggattcca tccgcctggt ggcggttttc aatgatgcgg
     5461 gtcagattac ccattcctgg cctgagg
//
LOCUS       DAOMSU010000136         5460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1316_length_5460_cov_2.936170, whole genome shotgun sequence.
ACCESSION   DAOMSU010000136 DAOMSU010000000
VERSION     DAOMSU010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1316_length_5460_cov_2.936170"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="PK077_06745"
     CDS             complement(<1..1202)
                     /locus_tag="PK077_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRN24036.1"
                     /translation="MDTPPTAKNWRLNAAAIIMDAEGCVLLGKDSGRNPYWHFPQGGV
                     IKHESIEQTLAREVWEEVGLRPTEYTIVSRLPGLRYKYPSNNRKVTRWIGQEQTYFLV
                     RCKTRRPKTDLHRSPEFSKTKWIPLQNLKLEMFPKFKRKVIKNALQQFFGPGFPSKHA
                     AVKTSSPSSPSSSTLTSRTMNRYLVPPGKKLRLKDYSPDDKSLFSGTKEESLIEFDKL
                     REELQELQKKLFAQHKHKILVILQAMDAGGKDGCVKHVFSRVDPQGLHVVPFKKPTTE
                     ELDHDFLWRVHKEVPAKGQIAIFNRSHYEDIIAVRVKKIFPDPVWKRRYKHVLDFESM
                     LAEEGTVIIKLFLNISKEEQKKRLESRLQDPDKLWKFCMDDLDDRNRWDEFQTAYQDL
                     IEKTSTPEA"
     gene            complement(1221..3881)
                     /locus_tag="PK077_06750"
     CDS             complement(1221..3881)
                     /locus_tag="PK077_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN24037.1"
                     /translation="MMQTFSRFRFPHAVLTSCAAVLLSLGGASPAAAAPSAGDTFPQD
                     RQDLLKNKKYQQGLKALENRLPLEASKHFQECLSSQNLAESQKAIIRPFLAEALIRAK
                     KTEEGLNAWEQLPDSPMKSYWTAVGLFNKGSFTKALEKLTAIPETDPLSLYGFQLKAQ
                     LARQLQDRQLLLETLSRLGQAESPAVSRPARLLLADVLVNQECYQDAATILEQLKTET
                     EAGTDSNRLIRSYTELIEGKLASKQGNLDQAIKTFSAVMDNKSYPEKIRDLGRLALAK
                     AEIAREKSNPEQAEEETRSQPQEEEAPHTAGTAEERLLAFIGGKAESALLMDAFNILL
                     EEDIFRTNPQALEKLNGWVKARDASRQPAAMYAMGSLLLEKDDLSGAVKMAEEGLSKY
                     PQSLPVQILSLNTITVLLDKNRLQDAERLISKYPGSSPDLVFQQGALAFLKKDYSLAQ
                     KLFREAARRASETTAENALFNENLAALNANDASGAAALIKEAAGSSRLRESILFEQAH
                     YAAKRMAPQAAELLANFITLAETPTLKTQARLDQVEVALNLNPPDLETVQAILPLLEK
                     EQLSPEQTMQLARLNILEEESRQEWPSAIQSCRRAIALDSEGKQADMLYLKLGELLYK
                     NGDFHEAQLVLQPFPSKYPDSPLQAAALFLAGKAAQQSNTGSTLNSALNIFKSLGAGD
                     TPFAQPARIEEASVLLRMGKADQCIAALENLLSSPLPRYMRLLALSIQADAWVAKEDA
                     HSDTLRKAINLCTEILDTPNLGLAWKFKALTQRAQFYERMNEQKKALDDYASILAHTP
                     SGSSANKRRDWHWFYNAGFASIRLLGQLQDWDGALALAKKLAQTSGPRAREAAAYARR
                     IQLEHFIWQDEPEASAPENGKPATPDQTAD"
     gene            complement(3889..4878)
                     /locus_tag="PK077_06755"
     CDS             complement(3889..4878)
                     /locus_tag="PK077_06755"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRN24038.1"
                     /translation="MPFLTADIVVRFPTENTALLDNRPQDFYMYVDRNFEGKKSQPWE
                     AGAYGFTRTLVRTQAGPVAVKFHEGIDIKPLRRDASGTPLDDVHPVAGGMVVHASANP
                     THSNYGRYVVIEHQLKDGPLYSLYAHLASVSCRKGDRVGTGNVIGKLGYSGVGLNKTR
                     AHVHLELCLKLQDDFENWYSSLKLGTPNRHGSYNGLNLAGFDPAPVLLQCKDGAEFSL
                     SRHISSLPVQYVVRAPSSGEPPSLVKRYPFLLKPGPADPKSWEISFTGEGVPVSVTPS
                     SQPCTEPVVIRAVPHPFSQLYRTCNRVSGSSKDPKLTAAGKRYIRLIFMGPES"
     gene            complement(4954..>5460)
                     /locus_tag="PK077_06760"
     CDS             complement(4954..>5460)
                     /locus_tag="PK077_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24039.1"
                     /translation="SFHMETGATDSKKFAFERDGHYYSKAPFISHKDIESYYSFPAPD
                     NTYGIVVNVRKGLGSRVEGITAANLGRQILPIVNGHAMQPLRLYSKPVTSGKLAVMGG
                     FSPADLAAMAEFIPPADPKREEPINKLAAVTKPLLPSKDPAQEKKEAKDPEEHKDRTV
                     ISTRRGRI"
BASE COUNT         1045 a         1334 c         1709 g         1372 t
ORIGIN      
        1 gcttccggag tggatgtttt ttcaatgagg tcctggtagg cggtttggaa ttcatcccag
       61 cggttgcggt catcaaggtc atccatgcag aatttccaaa gtttatccgg gtcctgaagt
      121 ctggattcca gccgtttttt ctgttcttcc ttggagatat ttagaaatag cttgatgatg
      181 acggttcctt cttccgcaag catggattca aagtcgagga catgcttgta gcggcgtttc
      241 cagactgggt ccgggaagat ttttttcacg cggacggcaa tgatatcttc gtaatgggaa
      301 cggttgaaga tggcgatctg ccctttggcg gggacttctt tgtgaacgcg ccacaggaaa
      361 tcgtggtcca gttcctcagt agtgggtttt ttgaagggta ctacgtgcag cccctgcgga
      421 tccacccggg agaagacatg cttgacgcag ccgtccttgc cccctgcatc catagcctga
      481 agaataacca gaattttgtg cttgtgctgg gcaaaaagtt ttttttgcag ttcctgcagt
      541 tcttccctca gtttgtcgaa ttcaatcagg gattcttcct tggttccgga gaagagagat
      601 ttgtcatccg gagaataatc ctttaaacgc agttttttgc ccggaggcac caggtaacgg
      661 ttcatcgtac gcgaagttag tgttgaagat gagggtgagg agggggaaga tgttttcaca
      721 gcggcatgtt tggaaggaaa gccgggcccg aagaattgct gaagggcgtt tttgatgact
      781 ttccttttga atttggggaa catttccagt ttgagattct ggaggggaat ccatttcgtt
      841 tttgaaaatt cggggctgcg gtgcaaatcc gttttagggc gtctggtttt gcagcgcaca
      901 agaaagtagg tttgttcctg gcctatccag cgcgtaactt tacggttgtt ggaagggtat
      961 ttgtaacgca ggccgggcag gcggctgaca atggtgtatt ccgtggggcg caggcccact
     1021 tcctcccata cctcccgcgc aagagtctgt tcaatgcttt cgtgtttaat gacgcctccc
     1081 tgcggaaagt gccagtacgg gttgcggccg ctgtccttgc ccagcaatac acagccttca
     1141 gcgtccatga tgatggctgc tgcgttgagt ctccagtttt ttgctgtggg gggggtatcc
     1201 atgacaggag gaaaggcttc ttaatccgca gtttgatctg gtgttgccgg ctttccgttt
     1261 tccggcgcgg aggcttccgg ttcatcctgc caaatgaaat gttccagctg gatgcggcgc
     1321 gcgtaagccg ctgcttccct ggctctgggg ccggaagtct gcgccagttt ttttgccagt
     1381 gccagggctc cgtcccagtc ctgaagctgg cccagcaggc ggatggaggc gaagccggcg
     1441 ttgtagaacc agtgccagtc cctgcgtttg ttggcggaac ttccgctggg cgtatgggcc
     1501 agaatggagg catagtcatc cagtgccttt ttttgttcgt tcatccgttc atagaactgg
     1561 gcccgctggg tcagagcctt gaatttccag gccagtccca ggttgggtgt gtccaggatt
     1621 tccgtacaga ggttgatggc cttgcgaaga gtgtccgaat gggcatcttc cttagctacc
     1681 caggcatccg cctggatgga gagggccagc aggcgcatgt agcgcggcaa tggggaggaa
     1741 agcaggtttt ccagggcggc gatgcattgg tccgctttcc ccatgcgcag cagaacggag
     1801 gcttcttcta ttctggcagg ctgggcaaag ggggtgtccc ccgcgcccag ggatttgaag
     1861 atgttcaggg cggagttcag cgtgctgccg gtattgcttt gctgggcggc ttttcctgcc
     1921 agaaagaggg ctgccgcctg aagagggctg tcaggatatt tggagggaaa gggctggagg
     1981 acaagctgcg cttcatggaa gtctccgttt ttgtacagaa gctctcccag tttgaggtaa
     2041 agcatatccg cctgtttgcc ttccgaatcc agggcaatgg ctcgtcggca ggactgaatg
     2101 gcgctgggcc attcctgccg gctttcctcc tccagaatat tcagccgcgc cagctgcatg
     2161 gtctgttccg gagacaactg ctctttttcc aacaggggaa ggatagcctg cacggtttcc
     2221 aggtccggag ggttgagatt cagggctact tccacctggt ccagccgcgc ctgggttttc
     2281 agggtcggag tttccgccag agttatgaaa tttgccagca attcagcagc ctgaggggcc
     2341 atccttttag cggcgtaatg ggcctgttca aagaggatgc tctcccggag ccgggaagag
     2401 cctgcggctt ccttgatgag agcggcagct ccggaggcgt cattggcgtt cagggcggcc
     2461 aggttttcat tgaacagggc gttttccgcc gttgtttccg aagcgcggcg ggccgcttcc
     2521 cggaaaagtt tctgcgccag ggagtagtct tttttgagga aagccagggc tccctgttga
     2581 aagacgaggt caggggatga accgggatat ttggaaataa gacgttccgc atcctgcaga
     2641 cggtttttat ccagcagaac agtaatggta ttcagagaaa gaatttgaac gggaaggctc
     2701 tgtggatatt tggagagccc ttcttccgcc attttgacgg cgcctgacag gtcatctttt
     2761 tccagaagaa ggcttcccat ggcatacatg gctgccggct ggcgggatgc atccctggct
     2821 tttacccagc cgttgagttt ttccagagcc tggggattgg ttcgaaagat gtcttcctcc
     2881 agcagaatgt tgaaggcgtc catcagcaag gcggattccg ctttgccgcc aatgaacgcc
     2941 aacaggcgtt cctccgccgt tccggcggtg tgcggagctt cctcttcctg aggctgggac
     3001 cgcgtttcct cttccgcctg ttccggattg cttttttcac gggctatttc cgccttggca
     3061 agagccaggc ggcccagatc acggattttt tccggatagg acttgttgtc cataacggcg
     3121 gagaaggttt tgatggcctg gtccagattt ccctgtttgc tggccagctt gccttctatg
     3181 agttcggtgt atgaacggat gagccggttg ctgtccgtcc cggcttccgt ttccgttttg
     3241 agttgttcaa gaatagtggc ggcgtcctgg taacattcct gattcaccag tacatccgcc
     3301 agaagaaggc gtgcggggcg ggagacggcg ggggattccg cctgccccag cctgctgagg
     3361 gtttccagta aaagctgccg gtcctgaagc tggcgcgcaa gctgggcttt caattgaaac
     3421 ccgtagaggg aaagcggatc cgtttccggg atagcggtaa gtttttccag ggctttggta
     3481 aaggagcctt tattgaagag gcccactgcc gtccagtagg atttcatggg agagtccggc
     3541 agttgctccc aggcgttcag tccttcttcc gtttttttgg cgcggatcag ggcttctgcc
     3601 agaaaggggc ggattatggc tttttgtgat tccgcaaggt tttgggaaga caggcattcc
     3661 tggaaatgtt tgctggcttc cagcggaagg cggttttcca gggctttcag tccctgctgg
     3721 tattttttgt ttttcaggag gtcctgcctg tcctgcggaa aggtgtctcc cgcagagggg
     3781 gcggcagctg caggggaagc gccgcccagg gaaagcagga cagccgcgca ggatgtgaga
     3841 acggcatggg gaaaacggaa tctggaaaag gtttgcatca tcgtaggatt atgattcagg
     3901 ccccatgaag atgagccgga tgtagcgttt gccggcggcg gtaagcttag ggtccttgct
     3961 ggagccagaa acgcggttgc aggtcctgta cagttgggag aaaggatgcg gaacggcccg
     4021 gatgacgacg ggttccgtgc acggttggct ggaaggagtt acggaaacgg gaaccccttc
     4081 tcccgtgaaa ctgatttccc aggatttggg gtcggcgggc cccggcttca gcaggaaggg
     4141 gtagcgtttg acaaggctgg gaggttcgcc ggaagaggga gcccgcacga cgtattggac
     4201 cggcagggag gagatatggc gcgagaggga aaattccgct ccgtccttgc attgcaggag
     4261 gacgggtgcc gggtcaaaac cggccagatt gagtccgtta taggaaccgt ggcggttggg
     4321 agtgcccagt ttcagactgg aataccagtt ttcaaagtca tcctgcagtt tgaggcagag
     4381 ttccagatgc acatgagcac gcgttttgtt caaacccacc ccggagtatc ccagctttcc
     4441 aatgacgttt ccggttccta cccggtcgcc tttcctgcag gagacggagg ccagatgggc
     4501 atacaggctg taaagcgggc cgtccttcag ctggtgctca atgaccacat agcggccgta
     4561 attgctatgg gtggggttgg cggaggcatg gactaccatg cccccggcta cggggtgcac
     4621 gtcgtccagc ggcgtgccgg aagcatccct cctgagaggt ttaatgtcga taccctcatg
     4681 aaatttgacg gctacggggc cagcctgggt tctgacgagg gtccgggtaa agccgtaggc
     4741 tccggcttcc cacggctggg atttcttccc ttcaaaattg cggtctacat acatgtaaaa
     4801 gtcctgcggg cggttgtcca gcagggctgt attttccgtg gggaagcgca ctacaatatc
     4861 cgccgtcagg aatggcatgc cccaaaggaa caggaggatg ccggtcagga gaggaaagga
     4921 tttgccgtgc atgctgatac gaggggtgct ggctcagatc cgcccccggc gggtgctgat
     4981 cacggtacgg tctttgtgtt cttcagggtc tttggcctcc ttcttttcct gagcagggtc
     5041 cttgctggga agaagaggct tggtaacggc ggccagtttg ttgatgggtt cttctctctt
     5101 gggatccgcc ggtggaataa attccgccat ggcggcaaga tccgccgggg agaaccctcc
     5161 catgactgcc agttttccag aggtgacagg tttgctgtaa aggcgcaggg gctgcatggc
     5221 atggccgttg acgatgggca gtatttgcct gcccaggttg gcggctgtta ttccttccac
     5281 ccggctgccc aggcctttcc ggacgttgac gacgatgccg taggtgttgt ccggagcggg
     5341 gaaggagtaa taggattcaa tgtccttatg ggaaataaag ggggccttgc tatagtaatg
     5401 gccgtccctt tcaaaggcga attttttgct gtccgtcgct cccgtttcca tatgaaagct
//
LOCUS       DAOMSU010000137         5446 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1320_length_5446_cov_2.496012, whole genome shotgun sequence.
ACCESSION   DAOMSU010000137 DAOMSU010000000
VERSION     DAOMSU010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1320_length_5446_cov_2.496012"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="PK077_06765"
     CDS             <1..266
                     /locus_tag="PK077_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRN24040.1"
                     /translation="SGSRNTGKILQRALNVHGAGLVVDGIPGKQTRQMLKTVLAQTGE
                     SEFLSTLNERRKSFYHSCKQFPVFGKGWLNRSNEAYRFAQSLI"
     gene            complement(406..789)
                     /locus_tag="PK077_06770"
     CDS             complement(406..789)
                     /locus_tag="PK077_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24041.1"
                     /translation="MNALSAAFSYYLAAGRRVEEMEEDGRLQDVYIRTPELVLMARRV
                     DSAAPFGKVVDVRFRFEPVRCDAWHLHFLAGDVRELLAYKREILSLPWILTQHGKRGD
                     GRLVKLTASRFCRLLGAFVGDEAFS"
     gene            complement(908..1222)
                     /locus_tag="PK077_06775"
     CDS             complement(908..1222)
                     /locus_tag="PK077_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24042.1"
                     /translation="MTEEQKKAALEAGKQGMKNAYEKSGTRPGLTWWERLLWVVLAGV
                     AYAASSLLSGCGHTVDVTADRAEICRDDSCLVLEPGHLSYSQKQPKTEMPPVIRKIRQ
                     GK"
     gene            1574..2458
                     /locus_tag="PK077_06780"
     CDS             1574..2458
                     /locus_tag="PK077_06780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRN24043.1"
                     /translation="MAKPALGKGFDALINQNLSRESLAAPQAGDVVHQLSHAAIIPSS
                     LQPRAIFTPEQLAELVDSIKEHGIIQPLIVRETQNGKYELIAGERRWRASGILGLSTV
                     PAIIREASDKDVLELALIENLQRENLSPLEEAAGYMRLKTEFRMKQGDIAKRVGKSRA
                     AVANSMRLLDLPQPVQDMLGNTFISVGHAKVLLSLKNKDQQIQLGRDIVNKGYTVRQT
                     EKAIQKMLNPPDPAPAKKPSSPQYKKISGILAKQFGTPVNISGQGSRGSIEITFSSKA
                     EFIRILELLGQDEWPDSK"
     gene            2478..3422
                     /locus_tag="PK077_06785"
     CDS             2478..3422
                     /locus_tag="PK077_06785"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRN24044.1"
                     /translation="MTPRIGLLLFTALLMQCENPVETPLPGVGTVPAQIRQTDCFNGG
                     NALFHAARITEMGNRASGTPGYRRQMDYLKEELTKYGWTCREQDFEKETPQGPVRFVN
                     LRARFGKAPNFLDPVRGLLTCHIDTKRGIDEFTGANDGASGAAAILETARILSGDPAR
                     ARNLELVFFDGEESFAEHMDSDDGLYGSRHYASSMQQPLPKWIINLDMVGRQGKKIRI
                     PAMTPQSMYRVYSRAIRELGYSPEEWGVSGYAILDDHVPFMERGVDTLNLIDDFQDGN
                     WWHTSKDNIGILGEKSFQQTGEMTLHILRQLLPGPPST"
     gene            3461..5443
                     /locus_tag="PK077_06790"
     CDS             3461..5443
                     /locus_tag="PK077_06790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HRN24045.1"
                     /translation="MSFNSPWLLWALFAASVPIIIHLVNRWRHRSVPWAAMEFLLRAA
                     RETRGTKKLLHYLILALRVMAVAALVTAFARPLLGSFFGWGSSGLNEVLLVLDRSASM
                     DARPDKTSSLRDAIPPLVESTFAQLGHCRLSLLDSATGTVTQIPAPEALADLTVSKTT
                     DGGADIPALLQKAIPYLEESGAGKTEIWIASDMQASSWKPDSPLWPGVRQRLAALPLP
                     PSIRIMALRDRPESNRGIRVRQAQVNNGKLVLDLEVLRQGFNPNQPEDVPVQLSVDNA
                     STAATLQLAGENTSIRKEIPLPQGKESGFGFVALPHDDFIRDDISFFTFAPRPLANIL
                     IFGPSGEVGKTLSLMSAPPGLASRKATMPGNTREAQANLASQSMVVWYGPPPRAEMGK
                     KLQTYIEEGGIALFLPDDTEHGTRHSFLGVSWGATETAPSEQYYRLETWDRQRGFLRD
                     GSDQTPIPANRLRAVRRKPLLGKYRTLASWDDGSCALAQVRAGTGSALFLGTLPRYSW
                     SNLADGHLLLPLLQRMADRGAERFSTSISLRVNDASLPQSATDTPVRLDNAQGSHPSG
                     SPADTAGVYRLGGQTYAVNRPWPEDMPDQITDEKLHLLLPEASISSMQSAEEAPTLVQ
                     EAWKLFLFFALACLLMEALLCLPGQTDKLPSPTTRP"
BASE COUNT         1192 a         1744 c         1441 g         1069 t
ORIGIN      
        1 attccgggag caggaatacc gggaagattt tgcagcgcgc gctgaatgtc cacggcgccg
       61 ggcttgtggt ggacggcatc ccgggaaaac aaacccggca gatgttgaaa accgttctcg
      121 cccaaacggg agagtccgaa tttctctcca ctttgaacga acggcgcaaa tcattttacc
      181 attcttgcaa acagttcccc gtgttcggca aaggatggct gaaccgcagc aatgaggcat
      241 accggttcgc acaatccctg atttagggaa gttcctgcaa aaaaactccc ggaaaaggag
      301 tgcaggagaa ttcaaccatt gttgcagcaa cctgcttcca tgcgccggat gccatgaaag
      361 agccaccgtg cagacatccg cacggaggct ctttcattaa cacgatcagg aaaacgcttc
      421 gtcccccaca aacgctccca gcagacggca aaaccgggaa gcagttaatt ttaccaatct
      481 cccgtccccc cgcttgccat gctgggtcag aatccagggc agggaaagaa tctcccgttt
      541 ataagccagc aattcccgta catctcccgc cagaaaatgg agatgccagg catcacaccg
      601 gaccggctca aaccggaagc ggacatcgac aacctttccg aaaggtgcgg cggaatccac
      661 cctgcgcgcc atcaggacaa gctccggagt acggatatag acatcctgga ggcggccgtc
      721 ttcctccatt tcttccaccc tccggcctgc agccagataa taggaaaagg cggcagataa
      781 agcgttcata cccagttttt tcttatgccg ccccaactgc tggctcaccg gacaattccc
      841 atcgggacgg ctcctgaaga gccgtttttc ctgaaaccgc cggggcaaaa gatcggacgg
      901 cacccagtca tttgccctgc cggattttcc ggataaccgg aggcatctcc gttttcggct
      961 gtttctggga gtaggacaaa tgccccggtt ccagcaccag gcaggaatcg tccctgcata
     1021 tctccgcccg atctgcagtc acgtctacgg tatgcccgca gccggagagg agggaggacg
     1081 ccgcataagc cactccggcc agaaccaccc acaagagacg ttcccaccag gtcaatccgg
     1141 gtcgtgttcc gcttttttca taagcgtttt tcatgccctg ttttcctgcc tccagagccg
     1201 ctttcttttg ttcttctgtc aaattgaagt ccatttgaga gagggggtga gtggtttcag
     1261 gaattctcgc gcatgttgtg ccgcaacggt tccgcgggga aaaatggcgc tgcggcagga
     1321 ctgtttattg ctattcattt tgttcttttt ttgttcattg cccggaaacg gcctttgccg
     1381 gcatggaacc ggcggtgaca tcagaccgct cccggacaca gacatccttg ccctgccctt
     1441 ggggcgggag caacacgcct gatacgtccg ggaacaagga atattccgcc agagacaaac
     1501 gccggaattt ccgccgcgga taacaaatac cctgttgaaa acggaaccgg ttcatgctag
     1561 tctcttccct gctatggcaa aaccggctct ggggaaaggc tttgacgccc ttatcaatca
     1621 aaatttgtct cgtgaatcct tggcggctcc ccaggcggga gacgttgtgc atcagctatc
     1681 ccatgccgcc attattccca gctccctgca gccgcgcgcc attttcacgc cggaacaact
     1741 ggcggaactt gtagattcca tcaaggaaca cggcatcatc cagcctttaa tcgtccgtga
     1801 aacgcaaaac ggcaaatatg aactgattgc cggggaaagg cgctggagag cctccggcat
     1861 cctggggctg tccaccgtgc ccgccatcat ccgggaagct tccgacaagg acgtgctgga
     1921 attggccctg attgaaaacc tccagcggga aaacctcagc ccgctggaag aagccgccgg
     1981 ctacatgcgc ctgaaaacgg agttccgcat gaaacaggga gacatcgcca agcgcgtagg
     2041 caaatcccgc gccgccgtag ccaacagcat gcggctgctc gacctgccgc agccggttca
     2101 ggacatgctg ggcaatacct ttatcagcgt agggcatgcc aaggtacttc tctccctcaa
     2161 aaacaaggac cagcaaatcc agctcgggcg cgacatcgtc aacaagggct acaccgtccg
     2221 ccagacggaa aaggccattc agaagatgct caacccgccg gatccggctc ccgctaaaaa
     2281 gccgtcctcc cctcagtaca agaaaatttc cggcatcctc gccaaacaat tcggaactcc
     2341 ggtgaacatc tccggtcagg gaagccgggg ctccatcgaa atcaccttct ccagcaaagc
     2401 ggaattcatc cgcatccttg agcttctggg ccaggatgaa tggcccgatt ccaagtaaac
     2461 ttcctccccc cttttccatg acgccccgca tcggacttct cctgttcacg gccctgctga
     2521 tgcagtgcga aaaccccgtg gaaacgcccc tgcccggtgt gggaaccgtt cccgcgcaaa
     2581 tccggcagac ggactgtttc aatggaggaa acgctctgtt ccacgccgcg cggataacgg
     2641 aaatggggaa ccgcgcttca ggcactcccg gttaccgcag gcagatggac tacctgaagg
     2701 aggaactgac caaatacggc tggacctgcc gggaacagga ttttgaaaag gaaacgcccc
     2761 agggccccgt ccgtttcgtc aacctgaggg cccgttttgg caaagctcca aatttcctgg
     2821 atccggttcg aggcctgctg acctgccata ttgacaccaa gcggggcatt gacgaattca
     2881 ccggagccaa tgacggagca tcgggagctg cggccattct ggagacagcc cgcattctgt
     2941 ccggagaccc cgcacgcgcc agaaatttgg agcttgtgtt ttttgacggg gaagaaagtt
     3001 ttgcggaaca tatggacagc gacgacggcc tgtacggctc caggcattac gcatcctcca
     3061 tgcaacaacc gctccccaaa tggataatca atctggacat ggtggggcgg cagggtaaaa
     3121 agatacgcat cccggccatg acgccccaat ccatgtaccg ggtttattcc cgcgctatcc
     3181 gtgaactggg ttattccccg gaagaatggg gagtatccgg ctatgccatc ctggatgacc
     3241 acgttccttt catggagcgc ggcgtggaca cgctgaacct gattgacgac ttccaggacg
     3301 gcaactggtg gcacacctcc aaggataaca tcggcattct gggcgaaaaa tccttccagc
     3361 aaaccggaga aatgaccctt catatcctcc ggcaactgct gcccggaccg ccctccacct
     3421 gaccaggcag ctctgaccct ccaattatcc ctgtcccgcc atgtccttca actctccctg
     3481 gctgctgtgg gctctctttg cagcctccgt tcccatcatc atccacctgg tcaaccggtg
     3541 gcggcaccgt tccgtcccct gggcggccat ggaattcctt ctccgtgcgg ccagggaaac
     3601 acgtggaacg aaaaaactgc tgcactatct gattctggcg ctgcgcgtga tggccgtggc
     3661 ggcactggta acggcctttg cgcggcccct gctcggaagc ttcttcggct ggggaagctc
     3721 cggattgaat gaagtcctgc tggttctgga tcgttccgcc tccatggatg cccgtccgga
     3781 caaaaccagc tcccttcggg atgccatccc ccctctggtg gaatccactt tcgcgcagct
     3841 cggccactgc cgcctttccc tgcttgattc cgccactgga accgttaccc agattcccgc
     3901 tccggaagct ctggcggacc tgactgtaag caagacaacg gatggaggcg cagatattcc
     3961 ggcacttctg caaaaagcca tcccgtacct ggaagaatcc ggcgcaggaa agacggaaat
     4021 ctggattgcc tcggacatgc aggcctcttc ctggaagcca gactcccctc tctggcccgg
     4081 tgtgcgccag aggctggccg cccttcccct gcccccttcc atccgcatca tggccctccg
     4141 cgacaggccg gaaagcaacc gcggcatcag agtccggcag gctcaggtaa ataacgggaa
     4201 actggtgctg gatctggaag tgctgcggca ggggttcaat cccaaccagc cggaggacgt
     4261 gccggtgcag ctttccgtgg ataacgcctc caccgctgcc acgctgcaac tggcagggga
     4321 aaacacctcc atccggaaag aaatccccct gccgcagggc aaggaatccg gcttcggctt
     4381 cgttgcgctt cctcatgacg acttcatcag ggatgacatt tccttcttca ccttcgcccc
     4441 caggcctctc gccaacatcc tgattttcgg tccttcggga gaagtgggga aaaccctttc
     4501 cctgatgtcc gcccctcccg gcctggccag caggaaagcc acgatgcccg gcaatacgcg
     4561 ggaagctcag gctaatctgg cctcccagtc catggtcgtt tggtacgggc cgccaccgcg
     4621 cgcggaaatg gggaaaaaac tccagacata cattgaagaa ggaggaatag cccttttcct
     4681 gcctgacgac acggaacacg gaacacggca ttccttcctg ggcgtttcct ggggcgcaac
     4741 ggagacggct ccgtccgaac aatactaccg tctggaaacg tgggaccggc aaaggggatt
     4801 cctccgcgac ggttccgatc agacacccat ccccgccaac cgtctccgcg ccgtgcgcag
     4861 gaaacccctg ctgggcaaat accgcactct ggcctcctgg gatgacggtt cctgtgccct
     4921 ggcccaggtc agggccggaa cggggtccgc tctgttcctg ggaacgctgc ccagatattc
     4981 ctggtccaac ctggcggacg gccacctgct gctccccctg ctccagcgta tggcggaccg
     5041 gggggcggaa cgcttttcca cctccatttc cctacgggtg aatgacgcct ccctgccgca
     5101 atccgccacg gatacccccg tgcgcctgga taatgcccag ggaagccacc cctccggttc
     5161 tccggctgat acggcgggcg tgtaccgact gggagggcag acctacgccg tgaaccgtcc
     5221 ctggccggaa gacatgcctg accagattac ggatgaaaaa ctccacctcc ttctgccgga
     5281 agcttccatc agttccatgc agagtgcaga ggaagccccc accctggtgc aggaagcatg
     5341 gaagctcttc ctgttcttcg ccctcgcctg cctgctgatg gaagccctcc tgtgcctgcc
     5401 cggccagacg gacaaactcc cctcccccac cacccgtcca tgaacc
//
LOCUS       DAOMSU010000138         5433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1325_length_5433_cov_2.905541, whole genome shotgun sequence.
ACCESSION   DAOMSU010000138 DAOMSU010000000
VERSION     DAOMSU010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1325_length_5433_cov_2.905541"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    214..290
                     /estimated_length=77
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <291..969
                     /locus_tag="PK077_06795"
     CDS             <291..969
                     /locus_tag="PK077_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN24046.1"
                     /translation="IQEFNNLPETTRKSYIAQFRKAEQLFAQKRIMECLFSLMELEKL
                     YAGNPGLYNLRGACYIEIRNIEKALENFEKAQKLDPSNLTIQFNLAEAHYVNHDYSRA
                     LKAFTELLLPFKDNPGMTPLLQFKRYICARKLDNQPLAAELEKLYGPMDDTPYYYCTQ
                     AILKLMEGDKEAAQEQLLSAIRIYGGTSAIQAFTDAMTEAGILPSPYGQTVPTEQPVK
                     TGLEKRR"
     gene            969..1505
                     /locus_tag="PK077_06800"
     CDS             969..1505
                     /locus_tag="PK077_06800"
                     /inference="COORDINATES: protein motif:HMM:NF023393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein O N-terminal
                     domain-containing protein"
                     /protein_id="HRN24047.1"
                     /translation="MNAEATVLKLYPLGENGLIAVWCTEEGLIRTAAKSTRKPGSPFA
                     GRLDIFYQCRMQWTQAKKGDLHTLTSADLLSPRLALRKSYLRLSAAGYFARLFLQMLE
                     PDTPIPEFYDLLQRAYTYLENNDPTLRAVLHFEQELARLHGIAHPGIPAHVILKSHFG
                     KLPPQREKLLGSLEQKES"
     gene            1657..2748
                     /locus_tag="PK077_06805"
     CDS             1657..2748
                     /locus_tag="PK077_06805"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HRN24048.1"
                     /translation="MKILDRYIARQLLGVTALGVMALSALLLLGNLFKELRPLLVENK
                     APISIVLEFIFQVIPFSLMFSIPWGFLTAVLLVYGRLASDNELTSMRMSGMSLWRLSA
                     PAIAIGIALSGLCYWINIDIAPRAKQSISELLIKAASINPKGLLNEGQAITKFDNLEI
                     YIDKRVDDVIHGMHIYQKADEKSPSVAMHSERVTMDFSPEKKILTLNLINPLITTQEE
                     GSISQSVTMDEMPLSINLDKSRSRRIKANRFTNREIREALDTPGYLDKKQTTEFATEL
                     PRRASFSLACIVFALIGVPLAINTRRKDTSTGFALGILIASLYFLALIFADLSRKNDT
                     MLPYILLWLPNIITVAVALHLHKRARHKG"
     gene            2797..4218
                     /gene="der"
                     /locus_tag="PK077_06810"
     CDS             2797..4218
                     /gene="der"
                     /locus_tag="PK077_06810"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRN24049.1"
                     /translation="MGASGNMMCMQHVPTIAIVGRPNVGKSAIFNRMAGRRIAIVHDE
                     PGVTRDRLSAPCKITDRACKIMDTGGIGARLGDGFAEQVEAEADIAIKTADLILFVLD
                     CRDHLTPIDQNIADHLRKSDIPVILLLNKADHEKQDLNLGEFTGLGFDDHIFLSAAHG
                     RGFSELASRLDGFLKQKGAPLKEELEEEPENGEETALPIKVAVVGRPNAGKSSLVNAI
                     LRDRRTIVSNVAGTTRDAIDIPYLHDGQPYVLIDTAGMRPRSRRDTSVEVFSAMRSEK
                     AIRRADICLLVIDIAAGITQQDRRIAGIIAEEGKPCIIIVNKFDLFHPNASRKDRMAE
                     VEEQVRRELFFINYAPFIATSAKKAEGVEIIFKVITRIRRESHNLPTTGQLNRLIQLA
                     QQMNPPGAASGSARRLKIYYATTAVDPKYNTIPVPRYVLFVNDKSLLTDSYSQYLRNK
                     IREAYPAPGIPVIFSARSRVRND"
     gene            4306..5205
                     /locus_tag="PK077_06815"
     CDS             4306..5205
                     /locus_tag="PK077_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN24050.1"
                     /translation="MSFDVAPGETLGIVGESGSGKSVTCYSIMGLIPMPPGRIESGSA
                     MLDGTDLLRCPEKELRAIRGKRISMIFQDPMTSLNPYLTIGEQVAEPLVIHEGAGKKE
                     ARTRALEQLALAGIPDAEQRMDAYPHQFSGGMRQRVMIAMALITRPEILIADEPTTAL
                     DVTVQKQVLDLIKKLQQDMGTSVILITHDLGVVRQYADRINVMYAGRIVESAPAKELL
                     EHPRHAYTRALMKSIPGLHAKGTPLYTIPGLPPNMTQEPCGCSFRPRNTLGNPALCLT
                     DREPELVEISPGHSVQNCPGCLT"
BASE COUNT         1251 a         1771 c         1287 g         1047 t
ORIGIN      
        1 tcgcatggcc ggaccaattc atcgaacacg gttcggaatc catcctcaga aaaaaatatg
       61 gactcacggc ggaagccgcg ctacaaaaaa tcattcccca cctgaactcc tgatctgttt
      121 atgaaaacac gcacctttct gctggcagcc cttgcgctcg cccttcttcc cgtctccgcg
      181 cgacaatccc tgccggcctc cccttcccct gccnnnnnnn nnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gattcaggaa
      301 ttcaacaacc tgccggaaac aaccagaaaa agctatatag ctcaattccg caaagcggaa
      361 cagctttttg cccagaaaag aatcatggaa tgtctttttt ccctcatgga gctggaaaaa
      421 ctttatgccg ggaatccggg cttatacaac ctccgcggcg catgctacat tgaaatccgc
      481 aatatcgaaa aggccctgga aaattttgaa aaagcccaga aactggatcc ctccaacctc
      541 accatccagt tcaacctggc ggaagcgcat tacgtcaatc acgactattc cagggctctg
      601 aaagctttca cggaactcct tctccccttc aaggacaatc ccggcatgac gcccctgctg
      661 caattcaagc gttatatctg cgcccgcaaa ctggataacc agcctcttgc cgcggaattg
      721 gaaaagctgt acggcccgat ggatgatacg ccgtactact actgcaccca ggccatcctc
      781 aaactgatgg aaggagacaa ggaagccgcc caggaacagc ttctttccgc catccgcatc
      841 tacggaggca ccagcgccat ccaggccttt acggacgcca tgacggaggc aggcatcctt
      901 ccctccccct atgggcaaac cgtcccaacc gaacagccgg taaaaaccgg actggaaaaa
      961 cgtcgttaat gaatgcggaa gcgacggtcc tgaagcttta tcccctggga gaaaacggcc
     1021 tgattgccgt ctggtgcacg gaagaaggcc ttatcaggac ggcggcaaaa agcaccagaa
     1081 aacccggcag cccctttgcc gggcgtctgg acattttcta tcagtgtcgc atgcagtgga
     1141 cgcaggcaaa gaagggggat ttgcacaccc tgacctccgc agacctgctt tcgccgcgcc
     1201 tggccctccg gaaaagctac ctccggctca gtgcggcagg ctatttcgcg cgcctttttc
     1261 tccaaatgct ggaaccggac acgcccatcc cggaatttta cgacttgctg cagagggcct
     1321 acacctacct ggaaaacaat gatcccacgc tacgcgccgt cctgcacttc gaacaggaac
     1381 tcgcccggct gcatggaata gcccacccag gcatcccggc gcacgtcatt ctgaaatccc
     1441 acttcggcaa gcttcccccg cagcgggaaa aactgctcgg aagcctggaa caaaaagaaa
     1501 gctgagcaac atgcctatcc cgctttcatc ctcagcaggg gatgttcccc gccccgctca
     1561 acacgtcctt cctccgggac gggatgcgca ttgttccgtg cgcgagattg acttgtccgc
     1621 agccttcggg cacaatatcc ggcacggttt tactgcatga agatccttga cagatacatt
     1681 gcccgccagc tgctgggtgt cacggccctg ggggtaatgg ccctgagcgc cctgctcctt
     1741 ctgggcaacc tgttcaagga actgcgtccc ctcctggtgg aaaacaaggc tcccatcagc
     1801 atcgttctgg aattcatctt ccaggtcatc cccttttcgc tgatgttctc cattccgtgg
     1861 ggattcctga cggccgttct tctggtgtac ggccgtctgg cctcggacaa cgaactcacc
     1921 tccatgcgca tgtccggcat gagcctgtgg cgtctgagcg cccccgccat cgccatcggc
     1981 atcgcccttt ccggcctttg ctactggatt aacatagaca tcgcaccgcg tgccaagcag
     2041 tccatttccg agctgctcat caaagccgct tccatcaacc cgaaggggct tctcaatgaa
     2101 ggccaggcca ttaccaaatt cgacaacctg gaaatctaca tcgacaaacg cgtggacgac
     2161 gtcatccacg gcatgcatat ctaccagaaa gcggacgaaa aatccccgtc tgtggccatg
     2221 cactcggaac gcgtgaccat ggatttctct cccgagaaaa aaatcctcac cctgaatctc
     2281 atcaatcccc tcatcaccac gcaggaagaa ggctccatct cccaaagcgt gaccatggac
     2341 gagatgcctc tgagcatcaa cctggacaaa tcccgctccc ggcgcatcaa ggccaaccgc
     2401 ttcaccaacc gggaaatccg ggaagctctg gacacgcccg gctatctgga taaaaagcag
     2461 accacggaat tcgccacgga acttcccagg cgcgcctcct tctccctggc ctgcatcgtc
     2521 tttgccctca tcggcgtgcc actggccatc aacacccgca gaaaagacac ttccacagga
     2581 tttgccctgg gcatcctgat cgcctcactc tactttctgg ccctgatctt tgcagacttg
     2641 tcccggaaaa acgacacgat gctcccctat attctgctgt ggcttcccaa catcatcacg
     2701 gttgccgtgg cgttgcacct tcacaaacgg gccaggcaca agggataagc cccctgcgga
     2761 agcccttccc gctggcgtgg aattagcttt cacagtatgg gagcttcggg taacatgatg
     2821 tgcatgcagc acgtccccac catcgccatc gtaggcaggc ccaatgtcgg gaaatccgcc
     2881 attttcaaca gaatggcagg caggcgcatc gccatcgtgc atgatgaacc cggcgtcacc
     2941 cgcgaccgcc tttccgcccc ctgcaagatt acggaccgcg cctgtaaaat catggatacg
     3001 ggcggtatcg gcgcgcgcct gggcgacggc tttgcagaac aggtagaggc agaggcggac
     3061 attgccataa aaacggcgga tctcatcctc ttcgtgctgg actgccggga tcaccttacc
     3121 cctatcgacc agaacatcgc ggaccacctc cgtaaatcgg acattcccgt tatcctcctt
     3181 cttaacaaag ccgaccacga aaaacaggac cttaatctgg gcgaatttac cggactgggt
     3241 tttgacgacc atatctttct ttccgccgcc cacggcagag gcttttccga attagcctcc
     3301 cggctggacg gttttctcaa gcagaaaggg gctccgctta aagaagaact ggaggaagaa
     3361 ccggaaaacg gagaggaaac ggcccttccc atcaaggtag ccgtagtggg acgccccaac
     3421 gcaggcaaat cctccctggt caacgccatc ctccgggaca ggcgcaccat cgtctccaat
     3481 gtggcgggca ctacccgtga cgccattgac atcccctatc tgcacgacgg ccagccctac
     3541 gtgctgattg acaccgccgg catgcgtccc cgctcccgtc gggacacctc cgtagaagtt
     3601 ttctccgcca tgcgcagtga aaaagccatc cgccgggcgg atatctgcct gctggtcatt
     3661 gacattgcgg cgggcatcac gcagcaggac cgccgcattg ccggcatcat tgcggaagaa
     3721 ggaaaaccct gtatcatcat cgtcaacaaa ttcgacctct tccatccgaa tgcctcccgc
     3781 aaggaccgca tggcggaagt ggaagaacag gtgcgcaggg aactattctt catcaactac
     3841 gcgcctttca tcgccacctc cgccaaaaaa gcggaaggcg tggaaatcat cttcaaagtc
     3901 atcacgcgca tccgccggga atcccataac ctgcccacta ccggccagct caaccgcctg
     3961 atccagcttg cccagcagat gaatcctccc ggcgccgcca gcggctctgc cagaaggttg
     4021 aaaatatatt acgccaccac agcggtggac cccaagtaca atactattcc cgtcccgcgc
     4081 tacgttcttt tcgtcaatga caaaagcctg ctcacggaca gctattccca gtacctgcgc
     4141 aacaaaataa gggaagccta ccccgctccc ggcattccgg tcatcttctc cgcccgttca
     4201 cgcgtgcgca acgactgatc atccctcctt ctccgcatgc tctccgtcag gaacctcagc
     4261 gcctccttcc acaccagggc cggcattgtc cgggcggtaa ggaacgtgtc ctttgatgta
     4321 gccccggggg aaacgcttgg catcgtgggt gaatccggat caggaaaatc cgtcacctgc
     4381 tactccataa tggggctcat tccgatgcct ccgggccgca ttgaaagcgg ttcagccatg
     4441 ctggacggca cagatctgct ccgttgcccg gaaaaggaac tccgggccat ccgcggcaaa
     4501 cgcatctcca tgatcttcca ggaccccatg acctccctca acccgtacct gaccatcggg
     4561 gagcaggtgg cggaacccct tgtcattcac gaaggggccg gcaaaaagga agcgcggaca
     4621 cgcgcgctgg aacagcttgc cctggcaggc ataccggatg cggaacaacg catggacgca
     4681 tacccccacc aattctccgg cggcatgcgc cagcgcgtga tgattgccat ggccctcatc
     4741 accagaccgg aaatactcat cgcggatgaa cccaccaccg cccttgacgt cactgtccag
     4801 aagcaggtgc tggatctcat caaaaaactt cagcaggaca tgggcacctc cgtcatcctc
     4861 atcacgcatg acctcggcgt ggtgcgccaa tacgcggacc gcatcaacgt catgtatgca
     4921 ggccgcatcg tggaaagcgc cccggcgaag gagcttctgg aacatccccg gcatgcctac
     4981 accagagccc tgatgaaatc cattcccggg ctgcatgcaa aaggcacccc cctctatacc
     5041 attcccggac tgcctcccaa catgactcag gagccatgcg gatgcagctt ccgccccagg
     5101 aacaccctcg gaaacccggc gctctgcctc acggaccgcg aaccggagct ggtggaaata
     5161 tctccggggc actcagtcca gaactgtccg ggctgtctga cttagtttcc tttcctgttt
     5221 gctcgcagat aatgctgcgt acaggaaaat ccttttaact gaattgattt ccgcacaatt
     5281 cttccagaat tccgtaaaaa cggcccacgt gcactccatc cactagcaca tggttcgcct
     5341 gaatggctac aggcatcacc atcctgccgg aagcatccgc ctccaccttc ccccaatgga
     5401 taaggggaaa gtattggtcc cggaacaccc tgt
//
LOCUS       DAOMSU010000139         5414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1335_length_5414_cov_2.874790, whole genome shotgun sequence.
ACCESSION   DAOMSU010000139 DAOMSU010000000
VERSION     DAOMSU010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1335_length_5414_cov_2.874790"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2085)
                     /locus_tag="PK077_06820"
     CDS             complement(<1..2085)
                     /locus_tag="PK077_06820"
                     /inference="COORDINATES: protein motif:HMM:NF019334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRN24051.1"
                     /translation="MKGKISLEDLVEQFLDQCRSGNVPDIQDFAGAHPEFASELLVLL
                     PLVMEMEDWSVEKKQGIMPPVHDFPDLEGDDYRLIRRLGSGGMGVVFEALQLSLNRKV
                     AVKLLSPLLLQDAAQRSLFEHEAHVIARLHHPNIVRIFSAACRPGCCYYAMELIEGKS
                     LDGCEFSNPYEIARIGLQAARAIAYAHRCGILHRDIKPANLLLDEAGEVHVSDFGLAF
                     LLQGRNEVIEKEGAQSGTLRYMSPERLVHGVNTFSSDQYAFGMTLYELVAKSPPFPGL
                     APEELMERICREPVPPLKCSEPDLAAIINKCVSFRPESRYRSMDELSEDLLHFLNHEP
                     VGASSPSPARRLQLWMKRKPAVAVLSLAAALCAAASVVALGAGYLQTASALKLAENNA
                     AVADAALSQVFTRIAEQPPSQKNTQLLSALLPYYRMISRGKNLPESKVCEANAVIGEC
                     ALRTGSYALAEEAFRNMMKFRKDAFPINQLATALKKQGKDKEATELFRQVADRFAMSE
                     RAEDRFETVRALLALSGSPESVERSRAFGMLETLLEDDPDHPEYRFQYAQLLAGNPRL
                     FRERRIPGIEPNAAVLLLQLADAHPERPEYGLALVELMLKKLRYARNFREHNQRELAD
                     TVNLSERLLGRWPNDPQIISGMVRLHARYIGALRREGKDAWARRESDRLQGILEVLFY
                     NPEISDAVKESLI"
     gene            complement(2082..2678)
                     /locus_tag="PK077_06825"
     CDS             complement(2082..2678)
                     /locus_tag="PK077_06825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRN24052.1"
                     /translation="MTQAETACPDAWGSHFLEYRGRLLALARRNLNPVLARRVSAEDV
                     VQEALLAACGKMAFFENNPEIPVYFKLRMILFQTLTAMERKHLQCLKRDVCREVNVTD
                     DGVGSAARLDWNRFADTVTGPLTRAVRADRYTLLRKAVEALPENDRQILELRHFDDMS
                     NADCAEVLHIGPKAASIRYVRALQRLRKQLMELTEFQP"
     gene            complement(2704..3543)
                     /locus_tag="PK077_06830"
     CDS             complement(2704..3543)
                     /locus_tag="PK077_06830"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRN24053.1"
                     /translation="MVKWLLCGAAMISGIVIAAETENRSFQFGNFEVIAVQDALSAMR
                     FELFPGMPEEEFLRLTGGGRVPSSVNVFLLKRGDGKIILVDAGNGGKRGGMLRKLEQS
                     GVFPESVDFILLTHMHGDHIGGLLGKHGEAVFSRALVYVSMPEWEFWQNGTAGPQGKQ
                     VRKVLHSYGSRVRTFRFGEEVLPGIKAFDASGHTPGHTVFETDSLLIVGDLLHAAALQ
                     IPRPEVCTIYDMNPSGAVQARRRFYEFAASSPKPVAGMHLPYPGIGRIGRSAAGYVYL
                     PGK"
     gene            complement(3709..3921)
                     /locus_tag="PK077_06835"
     CDS             complement(3709..3921)
                     /locus_tag="PK077_06835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24054.1"
                     /translation="MNKLIIPAIIGIFGLAPGTVTANAAESTTTSFSVQILKKKPKAL
                     KYGLKHGLKKGLKHGLKKGRINGGKK"
     gene            complement(4081..5067)
                     /locus_tag="PK077_06840"
     CDS             complement(4081..5067)
                     /locus_tag="PK077_06840"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRN24055.1"
                     /translation="MSINLQKVIIAVCAAATVSAGFAARTEGAKLHRFSTTIEKERPQ
                     LNEETKRLIASYRRDPSEANRSALRKQVGINYDKVLDRKKAKLEELKRTARHASKIQE
                     MQEIVDEMVQNREARIDQSMRRFSDPRFRPGARNADGGYLPVLGASWNVSIAYTPVTN
                     EDYAQFLKATGRKAPKDWDNGAMPAGKGRHPVVNVSYDDASAYCQWLSLQDGKAVYRL
                     PTEEEWEFAAGHMPKDADFNCGERNGTSPVDAHAATSGACGAIDMWGNCWEWTSSSVE
                     VSKAVAHGKTVMAVKGGSWRSPRTSCRTERKGEGRESSFAFSDVGFRVVREG"
BASE COUNT         1278 a         1593 c         1296 g         1247 t
ORIGIN      
        1 gatcaggctt tctttcacgg catcggaaat ctcgggattg taaaaaagaa cttctaggat
       61 gccctgcagg cggtcgcttt cccttcgggc ccaggcatct tttccttcgc gtcgcagggc
      121 cccaatatac cgtgcatgca gccggaccat gccggaaata atctgcggat cattcggcca
      181 acgcccgaga agcctttcgg aaagattcac cgtatcggca agttccctct ggttatgttc
      241 ccggaaattc ctggcatacc ggagtttctt cagcatcagc tctaccagcg ccaggccgta
      301 ttccggccgt tccggatggg cgtcggcaag ctggaggagc agcacggctg cattcggttc
      361 aatgccggga atgcgtctct cccggaaaag gcggggattt ccggccaaaa gctgtgcgta
      421 ttgaaaacgg tattcaggat ggtcaggatc atcctccaac aatgtttcca acattccgaa
      481 cgcccgggaa cgctcaacac tctccggaga accggacaaa gccagcaacg cgcggaccgt
      541 ttcaaaccga tcctccgccc gctccgacat cgcaaagcgg tcggccacct gccggaaaag
      601 ttccgttgcc tctttatctt taccctgttt tttcaaagcc gtcgccaact ggttgatggg
      661 aaaagcgtct ttcctgaatt tcatcatgtt gcggaacgct tcttccgcaa gagcataact
      721 cccggtgcgc agtgcgcatt ccccgatgac ggcattggcc tcgcaaacct ttgactccgg
      781 cagatttttt cctctggaga tcatcctgta atagggcagc aaagctgaaa gcagctgcgt
      841 atttttctgt gaagggggct gctccgcaat acgggtgaac acttgtgaca aagccgcatc
      901 ggcaactgcg gcattgtttt ccgccagttt cagcgcagac gccgtctgca gataaccggc
      961 gcccaacgcg acaacggaag cggccgcgca cagggccgca gccaaagaca atacggcaac
     1021 agccggttta cgtttcatcc acagctgcaa ccgcctggcc ggagacgggg aggaggcccc
     1081 gaccggttca tgattcaaaa aatgcagcaa atcttcagaa agttcgtcca tgcttcgata
     1141 gcgcgactcc gggcggaaac tgacgcactt gttaataatc gcggccaagt cgggttcgct
     1201 gcacttcaac ggcggaaccg gttccctgca aatccgttcc atcaattctt caggagctag
     1261 ccccggaaac ggcggggatt ttgcaacaag ctcataaagc gtcatcccaa acgcatactg
     1321 atcgctggaa aaggtattga ctccatgcac cagccgctcc ggggacatat accgcagcgt
     1381 tccgctctga gctccttcct tctcaataac ttcatttctc ccctgaagca gaaaagcaag
     1441 tccaaaatcg ctgacgtgta cttcccccgc ttcatccagc aacaggttcg ccggtttgat
     1501 atcgcggtgc agaatgccgc agcggtgggc atacgcaatc gccctggcgg cctggaggcc
     1561 aatacgggct atctcatacg gattactgaa ttcacatccg tccagactct tgccttctat
     1621 cagctccatc gcataataac aacatcccgg acggcacgcg gcactgaaaa ttctgacaat
     1681 attgggatga tgaagcctgg caatcacatg cgcctcatgc tcaaacaggc tgcgttgggc
     1741 ggcatcctgc agcagcaacg gagacaacag cttgacggcc actttccggt tgagggacaa
     1801 ttgcagcgct tcaaatacca cccccatacc gccgctgcct agtctccgaa tcagccggta
     1861 atcgtccccc tccaaatcgg gaaaatcgtg aaccggaggc ataattccct gttttttttc
     1921 aacgctccaa tcttccatct ccatcacaag cggaagaaga accagcaact cggaagcaaa
     1981 ctccggatgc gccccggcaa aatcctgaat atcgggaaca ttgccggaac ggcactgatc
     2041 caggaattgt tcaaccagat cttccagaga aatcttcccc ttcatggctg aaactcggtt
     2101 aactccatca gctgcttccg aagccgctgc agcgcccgca catagcggat gcttgcggct
     2161 ttcggcccaa tatgcaggac ttcggcacaa tccgcattgg acatgtcgtc aaaatggcgg
     2221 agttcaagaa tctgccgatc gttttcgggc agcgcctcga ccgctttccg gagcaatgta
     2281 taacgatccg ccctgacggc acgggtaaga ggcccggtca cggtatctgc aaaccggttc
     2341 caatccaacc gggcagcgga ccccacgccg tcatccgtca cattcacttc cctgcagacg
     2401 tctctcttga ggcattgcag atgcttcctt tccatagccg taagggtctg gaacagaatc
     2461 atccgcagtt tgaaatagac aggaatctcc ggattgtttt caaaaaaagc cattttcccg
     2521 caggctgcca gcagagcctc ctgaaccaca tcttccgccg aaacgcgccg ggcaagaacc
     2581 ggattcaaat tcctccgggc cagggcaaga agccttcccc ggtattcaag gaaatggctt
     2641 ccccaggcat ccgggcatgc tgtttctgct tgcgtcattg taccgtgtct tttttatcct
     2701 ttctcatttt ccgggcagat agacatatcc cgccgcactc cttcctatcc ggccgattcc
     2761 gggatacggc aggtgcattc cggcgaccgg cttcggggaa gaagccgcaa actcataaaa
     2821 gcgccgccgg gcctgaaccg ctccggacgg attcatatcg taaatcgtgc aaacctccgg
     2881 acgcggaatc tggagcgcgg cagcatgaag caaatctccg acaatcagca gggaatcagt
     2941 ctcaaaaacg gtatgcccgg gtgtatgccc ggaagcgtca aaagccttga tgccgggcag
     3001 aacttcttct ccaaaccgga atgttctcac ccggctgccg taagaatgca acaccttccg
     3061 gacctgtttt ccctgtggtc cggccgtacc attttgccaa aattcccatt ccggcatgga
     3121 aacatagacg agggctctgg aaaaaacggc ttcgccatgt ttcccaagca atcctccgat
     3181 gtggtcgccg tgcatatggg tgagtaaaat gaaatcgacg ctctccggaa aaacgccgct
     3241 ctgttcaagt ttacggagca tcccgccccg ttttcctccg ttccctgcgt ccaccagaat
     3301 gatttttccg tcgccgcgtt tcaacaggaa aacattcacg gaagaaggaa ccctcccgcc
     3361 tccggttaag cggaggaact cctcttccgg catacccggg aataattcga accgcatcgc
     3421 tgaaagagca tcctgaacag caatcacctc aaaatttcca aactggaatg aacgattttc
     3481 cgtctcagcc gcgatcacga tgccggaaat catggcggct ccgcacaaca accattttac
     3541 cataaaacgc ctttcttctc aatatgttga ctttttaaga catgatgggc aatgtatcaa
     3601 agctgtacgg aagtcagata tgtttccgca ctacaggtga taaaacatcc ttattgatgg
     3661 gaaaacgctg ccgggctgca ggcccggcag cagataagat ggcggcgact attttttacc
     3721 gccgtttatc ctgccttttt tcagaccgtg tttcaaacct ttcttgaggc cgtgtttcag
     3781 gccgtacttc aacgccttcg gtttcttttt cagtatttga acagaaaagg aggttgttgt
     3841 tgattccgcc gcgttggcag taaccgtacc gggagccaga ccgaatattc caataattgc
     3901 aggaatgatg agcttgttca tgataatcaa tgagggttga tattttttct gaaacggtat
     3961 cgtttcactt ttcaagaatg cagttcccta gggaaatcta ccaattcggc cggaatttcc
     4021 atcaggtctg ccagccagat tctattgcct gtaagaaact gaaacgcatg gaatcccccc
     4081 tcaaccttcg cggacgaccc ggaaaccgac atcactgaaa gcgaaagaag attcccttcc
     4141 ttctcctttc cgctcagtcc tgcagctcgt ccgcggcgaa cgccaggaac cgcccttcac
     4201 cgccatgacc gttttaccgt gtgccacggc cttggaaact tcaactgaag aagaagtcca
     4261 ttcccagcaa ttgccccaca tatcaatcgc tccgcaggcg ccggatgtcg cggcatgggc
     4321 gtccacggga gatgtcccgt ttctctcgcc gcaattaaaa tccgcatcct tgggcatatg
     4381 cccggcggca aactcccact cctcttcagt cggcaggcgg taaacggctt tcccatcctg
     4441 gagcgacaac cattggcaat aggcagaagc gtcgtcatag gaaacattca ccacgggatg
     4501 cctccctttg ccggcaggca tggcgccatt atcccaatcc ttaggagcct tccttcccgt
     4561 tgccttcagg aattgggcat aatcctcatt ggtaaccgga gtatatgcaa ttgatacatt
     4621 ccaggaagct ccgagcaccg gcaaataccc gccatctgca ttgcgggcac ctggtctgaa
     4681 tcgcggatca ctgaaacgcc gcatgctttg gtcgatgcgc gcttccctgt tttgaaccat
     4741 ttcatccacg atctcctgca tttcctgtat cttggacgca tgccgggccg ttcgtttcaa
     4801 ttcttcaagc ttcgcctttt ttcggtcaag gaccttgtcg taattaatcc cgacctgttt
     4861 cctcaaagcc gaccgattag cctcggacgg gtcccggcga taggaagcaa ttaaccgttt
     4921 cgtttcttca ttcaactgcg gcctctcttt ttcgatagta gtgctgaatc ggtgcagttt
     4981 ggccccctcg gtgcgggctg cgaatcccgc actgaccgtt gcggccgcac atacggcgat
     5041 aatgactttt tgcaaattga tgctcataac tgttccttct gtttatgata accgccgctc
     5101 ctctatggaa gacagaacgt tatcgtttca cttttaagaa tgctgctcct ccgggaattc
     5161 tacattcttt ttcaacaaag gtgctgccga agatgcggcg gaccggaaca ttggactgtt
     5221 tttcaaacaa ccagacggaa aaaaataaaa ggggccgcac cgcccgggaa tggtaacgga
     5281 aaacgccccc ggggaggcta taatggaaac tgccggcaac aatccgttcc gctcagggaa
     5341 gacaagcatg ataatctcat gcctgcttcc tgtgaaaaga gccggctccg gccttatttt
     5401 caaatttacc gctg
//
LOCUS       DAOMSU010000140         5405 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1338_length_5405_cov_2.557383, whole genome shotgun sequence.
ACCESSION   DAOMSU010000140 DAOMSU010000000
VERSION     DAOMSU010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1338_length_5405_cov_2.557383"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PK077_06845"
     CDS             <1..235
                     /locus_tag="PK077_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN24056.1"
                     /translation="DVTARGFLGPMGGGGEGRRSGLGTVLLISALKGLRELGYAYGVI
                     GGVGPADFYARAVGAMPIEGSSPGIYMDILPEP"
     gene            complement(313..894)
                     /locus_tag="PK077_06850"
     CDS             complement(313..894)
                     /locus_tag="PK077_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRN24057.1"
                     /translation="MKKNLLYLLAAGLSGTAFLHAADTVKLDQPDLERGAPIMKALSD
                     RQSIRSFSEKALSQKDLSDLLWAANGINRQESGKRTAPSAMNRQDVKIYICTKNASYL
                     YDHKAHAMIPVNDGDVRPADAPICLILVTDTAEPWAAMDAGIVSQNISLFCSGTGLAT
                     YPRASMNKDALAKALKLTSLQTPMLCHPVGYKK"
     gene            1198..2886
                     /locus_tag="PK077_06855"
     CDS             1198..2886
                     /locus_tag="PK077_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HRN24058.1"
                     /translation="MSTTELAELIDSKFRELREGSIVTGTIQEIRPQVVLVDIGYKSE
                     GAISISEFEDEEIEVGDQIEVLLERLENDEGIVVLSKEKAAHKQNWDKIVGVYRDGGL
                     VKGKVKSVVKGGLMVNVGVEAFLPGSQVDIIPPRDLNEYVGKVYEFKIVKVNDDRKNI
                     VLSRREVIEAERADQRQRFLETVKEGDKVEGIVKNITDFGAFVDLRGMDGLLHITDMS
                     WGRVNHPSEMLHIGQSLEVVILEVDREKERVSLGLKQMTDNPWADIERKYPINSHVKG
                     RVTKLLPYGAFVELEKGVEGLVHVSELSWVKRITRPSDVLKLDQEIEAVVLSISVKEQ
                     KISLGVRQLEDNPWADIESRFPIGTVIKGQVRNLTPYGAFVGLEEGIDGMIHVSDMSW
                     TRKINHPSEVLKKGDEVEAIVLEIKKEDQRVSLGIKQLESDPWESINDRFKVGDMVTG
                     QVAKIASFGAFVNLDGDIDGLIHISQLSEDHVERVKDVIKVGDEITARVIKVDSIERR
                     IGLSIKAVNYDTEQLRRETASFEALRPSSDMVGLEHAFNLATRENEEWSPSEEK"
     gene            3024..3425
                     /locus_tag="PK077_06860"
     CDS             3024..3425
                     /locus_tag="PK077_06860"
                     /inference="COORDINATES: protein motif:HMM:NF013490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HRN24059.1"
                     /translation="MLELTKSNEDYLEAIGLLSEKNGTAQVRDIAEMLKVKMPSVTSA
                     VKQLADMGLVEYTQYAPVKLTPQGRRIAGKIIVSHGILFDFLREELALPEERANEVAC
                     QIEHIMTFEEIEKLRSCRIRPFEGGEENSAK"
     gene            3771..4890
                     /gene="prfB"
                     /locus_tag="PK077_06865"
     CDS             join(3771..3854,3856..4890)
                     /gene="prfB"
                     /locus_tag="PK077_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_093286132.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRN24060.1"
                     /translation="MDPHIAADLSALDTAVLHSRLSELGSYLDLDGIQQRVAELDERM
                     SAPDFWDDQNAARALMAEVNPLKHRMEAFSALKSRLEDIDAAIELAQEADDDDLGREA
                     VEEFARWQKSLADFELLTLLNGPQDQASCYVTIHAGAGGTEACDWASMLLRMYIRWCE
                     RRGFSVTYLESTDGDDAGIRSVTLKVDGEYAYGYLKNERGIHRLVRISPFDSAGKRHT
                     SFASLDATPEVSDSINIEILDKDLKVDTYRSGGKGGQNVNKVETAVRITHIPSGVIVA
                     CQNERSQLRNKEEAMNMLRAKLYQIEEDKKQAEADRQYSEKGDIGWGNQIRSYVFQPY
                     QMVKDLRTGVESGNIQDVMDGNLDPFIEAMLRGHKRER"
     gene            4903..>5405
                     /gene="trmD"
                     /locus_tag="PK077_06870"
     CDS             4903..>5405
                     /gene="trmD"
                     /locus_tag="PK077_06870"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRN24061.1"
                     /translation="MAAKLTIDVLSLFPDMVEAPLGGSILGKARDRGLLEIRCHNIRD
                     WTTDKHRKTDDYLCGGGQGMLLKPEPIFAAVEELRRRETRVVLMTPQGRTFNQSLAAE
                     LAASGGHLIILCGHYEGVDHRVVEELVDMELSIGDYILTNGAIASVVVIDAVARLIPG
                     VLGDERSS"
BASE COUNT         1228 a         1271 c         1629 g         1277 t
ORIGIN      
        1 cgatgtaacg gctcgcgggt ttttgggccc catggggggg gggggagagg ggcgcagaag
       61 cggattgggg accgtgctat tgatttccgc cctgaaaggt ttgcgggagc tggggtatgc
      121 atacggtgtc atcggcgggg ttggtccggc tgatttttat gcccgggctg tgggcgcgat
      181 gcctattgaa gggagctctc ccggcatcta catggatatt ctgccggaac cataattccg
      241 cctgttgtgg aattcatttc catttctgat ggtttctttt ccggactgtc cggagaatgt
      301 agccggaagt agttattttt tatatcctac cggatgacag agcatgggtg tttggagaga
      361 ggttagtttc agggcctttg ccagggcatc cttgttcatg ctggcacgcg gataggtagc
      421 cagtcccgtc ccggagcaga acagggagat gttctgggag acaatgccgg cgtccatggc
      481 agcccagggt tccgccgtat ccgttacaag aatgagacat atgggggcat ccgccgggcg
      541 cacgtcccca tcgtttacgg gaatcatggc gtgggccttg tggtcataga ggtaggaggc
      601 gtttttagtg catatataaa ttttaacgtc ctggcggttc atggcggacg gggctgtacg
      661 tttgccggat tcctgacggt tgatgccatt ggccgcccag agcaggtctg ataaatcttt
      721 ttgggatagc gctttttcag aaaagctccg aatggattga cggtcggaca gggctttcat
      781 gatcggagct ccccgttcca ggtccggctg gtccagtttg acggtatccg cggcgtgaag
      841 gaaggctgtt ccggacagcc ccgctgcgag caggtaaagg aggttttttt tcatggtttt
      901 gcggcaggag tggctgatag atgcaggtat tccacatttc cccattagcc ggaatttaac
      961 atgggacgga agtgctacaa ggtttttgtg cgctcgtttt ctgccgccgt ccagaggggc
     1021 tccgcaggtt ccgtaaaaaa tcgggaggag tttttcaaga aaagccttga gttcttgtgt
     1081 gaaactgata ttgaaagcaa ccgatttttc gcccggcggg ggaaggcgcc ccgattgccc
     1141 gccggtcaat aaggggccat gagaaagatc gttaatacta actcatcaac catttaaatg
     1201 agcacaactg aactggcgga acttattgac agcaagttcc gcgaattgcg tgagggttcc
     1261 attgttaccg gaaccatcca ggaaatccgt ccccaagtcg ttttggtgga catcggctac
     1321 aagtccgaag gcgctatttc catttccgag tttgaagacg aggaaatcga agtgggggac
     1381 caaattgaag tccttttgga acgcctcgaa aacgacgaag gcatcgtcgt cctttccaag
     1441 gaaaaggccg cccataagca gaactgggat aagatcgtgg gcgtgtaccg cgatggcggc
     1501 ctggttaagg gtaaagtgaa gagcgtcgtc aagggcggtc ttatggtcaa tgttggcgtg
     1561 gaagctttcc tgcccggttc ccaggtggat attattcctc ctcgcgacct gaacgagtat
     1621 gttggaaagg tttacgaatt caagatcgtc aaggtaaatg acgaccgtaa aaatatcgtc
     1681 ctttcccgcc gtgaggtgat tgaagccgaa cgcgccgacc agcgccagcg cttccttgaa
     1741 accgtcaagg aaggcgacaa ggtggaaggt atcgtgaaga atatcacgga cttcggcgct
     1801 tttgtcgacc tccgcggcat ggacggcctg ctccatatca cggatatgag ctggggccgc
     1861 gtgaaccatc cgagcgaaat gctccatatc ggtcagtccc tggaagtcgt gattctggaa
     1921 gtggatcgcg aaaaggaacg cgtttccctg ggcctgaagc agatgacaga taacccctgg
     1981 gcggatatcg aacgcaaata cccgatcaat tcccatgtca agggccgcgt gaccaagctc
     2041 ctgccttacg gcgcctttgt ggaattggaa aagggcgtgg aaggcctggt gcacgtttcc
     2101 gagttgtcct gggtcaagag aatcacccgt ccgagcgatg tattgaagct ggaccaggaa
     2161 atcgaagccg tggttctttc catttctgtg aaggaacaga agatttccct cggtgtccgc
     2221 caattggaag acaatccctg ggcggatatc gaatcccgtt ttccgattgg taccgtcatc
     2281 aagggccagg ttcgcaacct tactccctac ggcgcttttg tgggactgga agaaggcatc
     2341 gacggcatga tccacgtgtc cgatatgagc tggacccgca agatcaatca tccctccgaa
     2401 gttctcaaga agggcgacga agtggaagcc atcgttttgg aaatcaagaa ggaggatcag
     2461 cgcgtctccc ttggtatcaa gcagcttgag tccgatccgt gggaatccat caatgaccgc
     2521 ttcaaggtgg gcgatatggt gactggccag gtggccaaga ttgccagctt cggcgccttt
     2581 gtgaatctgg acggcgatat tgacggcctg attcatatct cccagttgag cgaagaccat
     2641 gtggaacgcg tgaaggatgt gatcaaggtg ggtgatgaaa tcactgcccg cgtgattaaa
     2701 gtggacagca tcgaacgccg tatcggcctt tccatcaagg ccgtcaatta cgacaccgaa
     2761 cagctccgcc gcgaaaccgc ttcctttgaa gccctccgcc cgagcagcga tatggtgggg
     2821 ctggaacacg ccttcaacct ggctacccgt gaaaacgaag agtggagccc ttctgaagag
     2881 aagtaagccc gttttcgtat tacgctgatt aatttgaccg ccgcgaataa tcatattcgc
     2941 ggcggttttt tttctcattc caaagaaggc tggaaaaaat cttgagttag tttccactaa
     3001 agtattaact ttcattttat accatgttgg agctgaccaa gagcaatgag gattatctgg
     3061 aagctatcgg gttgctgtct gaaaagaacg ggacggccca ggtgagggat attgcagaga
     3121 tgcttaaggt aaagatgcct tccgttacat ctgccgtcaa gcagctggcg gatatgggac
     3181 tggtggagta tacgcagtat gctcccgtca agctgactcc tcaaggccgc agaattgccg
     3241 ggaaaattat tgtcagccac ggtattctgt ttgatttcct gcgggaggaa ttggccctcc
     3301 cggaagaacg cgccaatgaa gtggcttgcc agatagaaca catcatgact tttgaggaga
     3361 ttgaaaaact caggtcctgc cggatccgcc cttttgaggg tggagaggaa aattccgcca
     3421 agtagcatgg ctccgggctc tcttatggga agccgaggat atggatggcc gagccacatt
     3481 ccggaaagag ggggaagggc agaaaacgat gatgtaaggt aagggaatga aataagattt
     3541 ttgtctgctg tttttcccat ggggcattga aatggggaag tgggcttggt ttcggaaata
     3601 ttcatttcct gttagatgtt cttcctgcgg aagaggagcg gttttgttca gcgtgcggag
     3661 tgttattatt ctcggaaatg tcccggtacg agtcggagta aaagaggatg acttggagaa
     3721 acggcatttt gcccttttcc gtccaggtaa ttcgtgatag gttcccgcgc atggatcccc
     3781 atatcgctgc agatctctcg gcgctcgaca cggctgtcct gcatagccgt ctttccgagc
     3841 tcgggagcta tctttgacct ggacggcatt caacaacgag tagccgaatt ggatgaacgc
     3901 atgagcgccc cggatttctg ggatgaccag aatgccgcac gggcgctgat ggcggaggtg
     3961 aatcccctga aacacaggat ggaggcgttt tccgcgctga agtcccgtct ggaggatatt
     4021 gatgctgcta ttgagctggc tcaggaggcg gatgacgatg acctgggccg ggaggctgtg
     4081 gaagagtttg ccagatggca gaagtctctg gcggattttg aattgctgac cctgctgaac
     4141 ggaccgcagg accaggcttc ctgttatgta accatccacg ccggggccgg cggcacggaa
     4201 gcctgtgact gggcatccat gttattgcgc atgtatatcc gctggtgtga gcgccgggga
     4261 ttttccgtga cgtatttgga gagtacggac ggggatgacg ccggcattcg ttccgtgaca
     4321 ttgaaggtgg atggagagta tgcctacgga tatttgaaga atgaacgcgg catccaccgg
     4381 ctggtgcgca tttctccctt tgattccgcc gggaagagac atacttcttt cgcttccctg
     4441 gacgcgacgc cggaagtttc tgattccatc aatattgaaa tcctggacaa ggatttgaag
     4501 gtggatacct accgttccgg cggcaagggc ggccagaacg tgaacaaggt ggaaaccgcc
     4561 gtgcgtatca cgcatattcc ttccggtgtg atcgtggcct gccagaatga gcgcagccag
     4621 cttcgtaaca aggaagaggc tatgaatatg ctgcgtgcca agctatacca gattgaggaa
     4681 gacaaaaagc aggcggaagc cgaccgccag tacagtgaga agggagacat cgggtggggc
     4741 aaccagatcc gttcctatgt tttccagcct tatcaaatgg tgaaagattt gcgcacagga
     4801 gtagagtccg gcaatattca ggacgtgatg gacggcaacc tggatccgtt cattgaggcc
     4861 atgctgcgcg gccataagcg cgaacgctga tgtttttttc ttatggccgc caaactgacg
     4921 attgacgttt tgtccctgtt cccggatatg gtggaagccc ctctgggcgg cagcattctg
     4981 ggaaaggccc gtgacagggg attgttggag atacgctgcc ataatatccg tgactggacg
     5041 acggacaagc accgcaaaac ggacgattac ctttgcggag gggggcaggg catgctgctg
     5101 aagccggaac ctatctttgc cgccgtagag gaacttcgcc ggcgggaaac ccgcgttgtg
     5161 ctgatgactc ctcagggccg tacgttcaac cagtcattgg ccgcggaatt ggctgcctcc
     5221 ggaggccatc tcattatcct ctgcggtcat tatgaggggg tggaccatcg tgtagtagaa
     5281 gagctggtgg atatggaatt gtccatcggg gactatattc tcacgaacgg ggccattgcc
     5341 tctgtggtgg taattgacgc cgtagcgcgc ctgattcccg gcgtgctggg ggatgagcgt
     5401 tcttc
//
LOCUS       DAOMSU010000141         5399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1340_length_5399_cov_2.768525, whole genome shotgun sequence.
ACCESSION   DAOMSU010000141 DAOMSU010000000
VERSION     DAOMSU010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1340_length_5399_cov_2.768525"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            306..1088
                     /locus_tag="PK077_06875"
     CDS             306..1088
                     /locus_tag="PK077_06875"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HRN24062.1"
                     /translation="MNTNNLINKVILCSLAMAGAALLPSCVSPKEVLYIQDVSGNSRE
                     TIKANYQTTIQKDDQLYIAVSSKQPELTAPFAMSEIGSSSGTSSNKPKGYLVDAQGCI
                     VLPVIGKMKAAGKTCSQLASDIAATLKNNDYIRDASVNVQIMNFKFSVLGEVNAPGTY
                     TIEGQRVTILEAISRAGDLNIDGNRDVTLIRETNGTRQIASIDLRSKDLFTSPYYYIQ
                     QNDIIYVTPSDRKVNTRSDAAQWYGWGLSGLGVTLAVIALCL"
     gene            1219..3615
                     /locus_tag="PK077_06880"
     CDS             1219..3615
                     /locus_tag="PK077_06880"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRN24063.1"
                     /translation="MANNTAPTPPVTENTEETTSLDTVLLVLRRYWFIIILAALAGGT
                     AAYYLAGRQNYIYQKTASVLMRDAKTSSGASSERIMAELGIDSGAANLANESFILKST
                     ALMKKVVEDLNLNTSYWQKKDFREIDLYKTSPLLVHFERIDKQRACTLQITPLDEKRF
                     MLSHSNNQGESVQLEGFYGKPLTLPFAVISIHPTSLMTDAWNEKTVIVRHSPVLETAN
                     ALLRGLTITRPDSKESSLLEMTLTSSNPQKAEDTLNHLIQVYNQISKDERNKAALKTE
                     NFIQSRLKELGAALSDVDKKITEFKTKSDIVKDTDTTMSADFSTSQALEKEIFDLETQ
                     IKLAAILADNLKETERKQGLISVETGLPDSGIARQIEHYNEAYLEYQKIAGSAGSQNP
                     ITVSLRDRMNSTRAAANKALSNYRSNLNLKLNQLISKRNSLTERLTETASREQEIIPL
                     VREHKVKEELYLMLLSKEQENALAMAVTESSARVLETAHGSNFPISPKTIQYIAGGTA
                     GGALFSIFAFMGAAMLNSKVNNKHDLPSTNRQPVIAELPQMNKKESRNTGLFIQDEHS
                     VIAECFHILRNNVDSMLPRPEQGGHVILVTSTLPGEGKTFTSANLAAAFAYAGKKVLL
                     IDGDFRKSSLTRRLGGSGRKGLTSILLQQSSDTTGTIRPLGENSRGMDILYTGPMVPN
                     PVTLLSHPLLGQILGILKKQYDAVIIDAPPYGILADTAILASHSDITLYTVRSGKIDK
                     RYLTQIQQLADQGKLPNMAYIINGVNFKSSSYSYYGYGYGYQYGYGSGEPRQTTRKQD
                     "
     gene            3682..4353
                     /locus_tag="PK077_06885"
     CDS             3682..4353
                     /locus_tag="PK077_06885"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24064.1"
                     /translation="MFGNLFTPRYATSSLFPDGMDMHTHILWGVDDGAACLEESLGII
                     RRLKAMGLKGAYCTPHIMARYPDNTPDFLRRRFDQLLADTREEQFQLRLAAEYMLDNQ
                     FNEQLRRYTPLTYDGIHLLVELPQYYLPGAWKDMISSVRDMGYVPVLAHPERYGRLLQ
                     QEDFLQLARQEGVKYQGNVGSLKGFYGKSAAALAQTFRTRQLYEWWGTDSHNIAMAKR
                     MPLKN"
     gene            4392..5240
                     /locus_tag="PK077_06890"
     CDS             4392..5240
                     /locus_tag="PK077_06890"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRN24065.1"
                     /translation="MSAPITNIPFSNPLYRNHNFYRRYGKRALDFTGALFGLICLFPV
                     LAILAILLAIVQRTSPFFLQKRIGHGLKPFHIIKFKTMKDTRDKEGNLLPDEERTTVL
                     GSLLRSTSLDELPELINVLLGDMSFIGPRPWIPDQMAIFTPSTRKKRMSLRPGITGLA
                     QIRGRNNLTFRQRVCYDLSYQRHLTFRYDLNILFYTFLKVVEREGIQQRPDALSTPAR
                     VLKPRDLMTYGLKENTQLILRSIRKEPVPSLSVPAHPLIPKDLPTKGLRANTQLIMRS
                     RQKSVQ"
BASE COUNT         1439 a         1649 c         1181 g         1130 t
ORIGIN      
        1 tatcttttca ttattgatga ttattttccc gatttttcca tgtgagggga aatatatccc
       61 cctttttcaa acccttgctt gtttcaaaca aaaaaaatca aaatcatcaa atttaaaatg
      121 atgactgaac atatagcggt ctttttcttt caaacttccg gaagattaaa agcatggagg
      181 gcatcacgcc attcccgtgg tgcttccggg ccaaggaaga aaatcctttg agccggaatg
      241 gcctctttcc ctgtttttat cgccatctta cctcccaaaa gcaacgtttc ctcctccctc
      301 accaaatgaa tacaaacaac ctcatcaata aagtcattct gtgctccctg gccatggccg
      361 gagccgctct cctgccctcc tgcgtcagcc ccaaggaagt cctctatatc caggatgttt
      421 ccggaaactc cagggaaacc atcaaggcaa actaccagac aaccatccag aaggatgacc
      481 aactctatat cgcagtcagc agcaagcagc cggagctcac cgcccccttt gccatgtctg
      541 aaatcggttc ctccagcggc acctccagta acaaacccaa aggatatttg gtggatgccc
      601 agggctgcat cgtcctgccc gtcatcggca aaatgaaggc tgcgggcaaa acctgctccc
      661 agctggcctc cgacattgcc gctactctca agaacaatga ctacatccgg gatgcctccg
      721 tcaatgttca gatcatgaac ttcaaattct ccgttctggg cgaagtcaat gctcccggaa
      781 cctacactat tgaaggacag cgcgtaacca ttcttgaggc catcagccgg gccggagacc
      841 ttaatattga cggcaaccgg gacgtcacgc taatccggga aaccaacgga acccgccaaa
      901 tcgccagtat cgacctgcgc agcaaggatt tgttcacttc tccttattat tacatccagc
      961 aaaacgacat catttacgtg acaccctcag accgcaaggt caacacgcgc agcgacgctg
     1021 ctcaatggta cggctggggc ctctccgggc ttggtgtcac tctggccgtg atcgccctgt
     1081 gcctgtaacg gacattccgc ctctttctca tccggcttaa tcatacctgt gaacggaaat
     1141 atagggacgg tccgattgca tcggaccgct tccccagtgg tccagtattt ccaaacaaca
     1201 ttccaccctc tttcctccat ggcaaataat accgctccca ctccgcctgt tacggaaaac
     1261 acagaagaaa caacttcctt ggacacggtg ctgctcgtcc tgcgccgcta ctggtttatc
     1321 atcattctgg cagccctcgc cggaggtacg gcggcttact acctagccgg ccgacagaat
     1381 tacatttacc agaagacggc cagcgtcctg atgcgcgatg ccaaaacgag cagtggcgca
     1441 tcttcggaac gcattatggc agagctgggc atcgattccg gcgcagccaa cctggccaac
     1501 gaaagcttca tcctcaaatc caccgcatta atgaaaaaag tggtggaaga cctcaatctc
     1561 aacacttcct attggcaaaa aaaagatttc agggaaatcg acctctacaa aaccagcccc
     1621 ctgctggtcc attttgaacg gatcgataag caacgagcct gtaccctgca gatcacgcct
     1681 ctagatgaaa agaggttcat gcttagccat tccaataacc agggggaatc agtccagctg
     1741 gaagggtttt acggaaaacc tcttacactt ccctttgccg tcatttccat ccatccaaca
     1801 tccctgatga ccgatgcgtg gaatgaaaaa accgtcatcg taagacactc tcccgttctt
     1861 gaaaccgcca acgccctgct ccgtggcctg acaattaccc gtccagactc caaggaatcc
     1921 agccttctgg agatgactct gacatccagc aatcctcaga aagccgaaga cacgctcaac
     1981 caccttatcc aggtttacaa ccaaatttcc aaggatgaac gaaacaaggc ggcccttaaa
     2041 acggaaaact tcatccagag ccgcctgaaa gaactcggag ccgccctgag tgacgtggac
     2101 aaaaagatca ccgaattcaa gacgaaaagc gacattgtca aggatacgga taccaccatg
     2161 agcgcggact tcagcacgtc ccaggcgctg gaaaaagaaa ttttcgatct cgaaacacaa
     2221 atcaaactgg cagccatcct ggctgacaat cttaaggaaa ccgaacgcaa acagggcctg
     2281 atttccgtgg aaaccggact tcccgattcc ggtattgccc ggcagataga acactataac
     2341 gaagcctacc tggaatatca gaaaattgca ggaagcgccg gttcccagaa cccaattacg
     2401 gtgagcttgc gcgacaggat gaattccacc agggcggccg ccaataaagc tctttccaac
     2461 taccgtagca atttgaatct caagctcaac cagctcatca gcaaacggaa ttccctgact
     2521 gaacgactca cggagacagc aagcagggag caggaaatca ttcccctggt ccgcgaacac
     2581 aaggttaagg aggaacttta cctgatgctc ttaagcaagg aacaggaaaa cgccctggct
     2641 atggcggtaa cggaatccag cgcccgggtt ctggaaaccg cccatgggtc caactttccc
     2701 atttccccaa aaaccattca atacatcgcg ggaggaacgg cgggaggtgc cctgttcagc
     2761 atatttgcct tcatgggagc ggccatgctg aacagcaagg ttaacaataa acacgatctc
     2821 ccgtccacaa acaggcagcc tgtcattgcc gaactgcccc agatgaacaa aaaagaaagt
     2881 agaaataccg gtcttttcat tcaggacgaa cattccgtca tcgcggaatg cttccacatc
     2941 ctgcgcaata acgtagattc tatgctcccc aggccggaac agggaggaca cgtcattctg
     3001 gttacctcta ccctccccgg agaagggaaa accttcacct ccgccaatct ggccgccgct
     3061 ttcgcctatg ccggcaaaaa agtactgctt attgacggag atttccgcaa atcctccctg
     3121 acccggcgtc tcggcggttc cggacgcaaa ggactcactt ccattctgct ccaacagagc
     3181 tccgacacca ccggcaccat tcgcccccta ggggaaaact cccgcggcat ggatatcctt
     3241 tacaccggcc ccatggtacc caatccggtt accctgctca gtcatcctct gctgggtcaa
     3301 atcctgggca tcctgaaaaa acaatacgac gccgttatca tcgacgctcc gccctacggc
     3361 attctggctg ataccgccat tctggcatcc catagcgata tcaccctgta caccgtgcgc
     3421 agcgggaaaa tcgacaaacg atacctaacc cagatccagc aactggccga tcagggaaaa
     3481 cttcccaata tggcgtacat catcaacggc gtcaacttca agtcctccag ttacagctac
     3541 tatggctacg gatacggtta ccaatacgga tacggctccg gagaaccacg gcaaaccacc
     3601 aggaaacaag attaataaga cacaggcaat gcataagccc aaggctttct tccctgctct
     3661 ttactctttt cggcagcaat catgttcggc aacctgttca ctccccggta tgcgacatcc
     3721 agcctctttc cggacggcat ggacatgcac acccatattc tatggggagt tgacgacgga
     3781 gccgcctgcc ttgaagaaag cctgggaatc attcgccggc tcaaggccat gggactcaaa
     3841 ggagcttact gcacccccca tatcatggcg cgctacccgg acaatacgcc cgatttcctg
     3901 cgccgccgtt ttgaccagtt gcttgccgac accagggaag aacaattcca gctccggctc
     3961 gcggcggaat acatgctgga caaccagttc aatgagcagc tgcgccgata cactcccctg
     4021 acctacgacg gaatccacct gctggtggaa ctgccccagt actaccttcc tggcgcctgg
     4081 aaggacatga tcagctccgt tcgggacatg ggatacgtcc ctgtcctggc gcaccctgaa
     4141 cgctatggcc gcctcctgca acaggaagat ttcctccaac tggcccggca ggaaggcgtc
     4201 aaatatcagg gcaatgtcgg ctccctgaaa ggattttacg gcaaatccgc cgcagccctg
     4261 gcacaaacgt tccgcacccg tcaactttac gaatggtggg ggacagacag ccacaacatc
     4321 gccatggcca aacgcatgcc tctgaaaaac taactccgcc taccctatta acaacctctc
     4381 gtactacacc catgtcagcc cctataacta atatcccctt cagcaacccg ctgtatcgta
     4441 atcataattt ttacagacgc tacggaaaac gcgccctgga tttcacgggt gcactcttcg
     4501 gccttatatg cctgtttccg gtattagcca ttctggctat tctgctggcc atcgtccaga
     4561 ggacatctcc attcttcctg caaaagcgca tcgggcacgg cctcaaaccc tttcatatca
     4621 tcaaattcaa aaccatgaaa gacaccaggg acaaagaagg gaacctgctc ccggatgaag
     4681 aacgcacaac agtcttgggc tccctgctgc gcagcacctc cctggacgaa ctgcctgaac
     4741 tcatcaacgt tctgttgggg gacatgagct ttatcggccc acgcccctgg attcccgatc
     4801 aaatggccat ttttacccct tccacccgga aaaaaagaat gtccctgcgc ccgggcatca
     4861 cggggctggc ccagattcgc gggcgcaaca acctcacctt ccgtcagcgg gtatgttatg
     4921 acctgagcta ccaacgccac ctgaccttcc ggtacgacct gaatatcctt ttttacacct
     4981 tcctcaaggt tgtcgagagg gaaggtattc agcagcgtcc ggatgccctg agtacaccgg
     5041 cccgtgtact gaagcccaga gatttgatga cctacggatt gaaagaaaat acgcaactca
     5101 ttttaagaag tatccgaaaa gaacctgtcc cctccttgag cgtacccgcc catcccttga
     5161 tccccaaaga cttgccgacc aaaggtctca gggcaaatac ccagcttatc atgaggagcc
     5221 gtcagaaaag cgtccaataa aatttcctct cccccccccg ccaaaaatga aaatttcagt
     5281 cattacggtt tgttataaca gcattgcaac actgcaagac acgctggaaa gcattctgcg
     5341 gcaaacctac ccggacgtgg aaagcattgt ggtggacggg gccagcaagg acgggaccg
//
LOCUS       DAOMSU010000142         5389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1345_length_5389_cov_2.635358, whole genome shotgun sequence.
ACCESSION   DAOMSU010000142 DAOMSU010000000
VERSION     DAOMSU010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5389
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1345_length_5389_cov_2.635358"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PK077_06895"
     CDS             complement(<1..916)
                     /locus_tag="PK077_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRN24066.1"
                     /translation="MNSDQFETGIPAPQGLYDFEQERDACGVGLVADLKNEPSHKIIE
                     MGITVLKRLMHRGAVGSDPDTGDGAGILLALPDEFFRLALPNKLPARGKYGVAMMFGG
                     CGHEEELEAAVTENGGSVIAWRQVPVDRDSIGKNAQRTCPLIRQLFIDGSGFADQAEF
                     ERKLFVMRREMERRVEGCYVCSCSSRSIVYKGLFLGTQIEGFYGDLASEHFKSPLALV
                     HQRYSTNTFPTWSLAHPFRYLAHNGEINTLRGNLNHLSVREPHLSSTLLGDDLQKLLP
                     LIPPGQSDSACLDNMVELLAAAGRDLRHV"
     gene            1313..3448
                     /locus_tag="PK077_06900"
     CDS             1313..3448
                     /locus_tag="PK077_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRN24067.1"
                     /translation="MMSDSTRTQAISAIASLPVGGASESAPVSIDYYGADVFSTEVMK
                     KYLPKDTAKTLLATIQDGLPLNADIAADVAHAMKQWALERGATHYTHWFQPMTGSTAE
                     KHDSFLDPKGMEPIMSFSGKNLIVSEPDASSFPSGGLRCTFEARGYTAWDPTSPAFIK
                     RHGNGATLCIPTAYCSYTGDALDKKTPLLRSRQALGNAVKKLMKCFGLPDEHVTITLG
                     PEQEYFLIDKNFYLNRPDLVQTGRTLFGAPPAKHQQLEDHYFGSIKPRVLNFMSDVEQ
                     ELWRLGIPAKTRHNEVAPAQFELAPLFEDVNLAIDHNMLVMEVLRQQASKHGLVCLLH
                     EKPFAGVNGSGKHNNWSISYGNKNLLDPGSDPQQNAIFLTVLTAIIEAVDKHSDLLRN
                     SVASAGNDHRLGANEAPPAIISIFLGAQLNEVIENMINGSSGCGKRNDTLKIGVDTLP
                     VLPRDATDRNRTSPFAFTGNKFEFRAPGSSQSCAGPMMTLNTIVAESLDSLAEELSSF
                     APETFLPQLQETLKRRISEHKRIIFNGDNYSEEWVKEAERRGLPNLKNTMTALHTLVN
                     EKNVALFEKYGVFSRRELESRFEIFLEEYHRRIRIEGRLSWEMAATIILPALRNEYEQ
                     TVSALSRALDAKRTSGTAALQKLADKLGDALDSIVSDLDTLETALTSCHEDILVAMSQ
                     LRTSVDAAETLVNDRSWPLPKYREMLFIY"
     gene            3701..>5389
                     /gene="carB"
                     /locus_tag="PK077_06905"
     CDS             3701..>5389
                     /gene="carB"
                     /locus_tag="PK077_06905"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HRN24068.1"
                     /translation="MPKDTSIKKILVIGSGPIVIGQGCEFDYSGTQACKALREEGYEV
                     VLVNSNPATIMTDPETSPRTYIEPITPEFVEKIIIREKPDALLPTLGGQTALNCAMEL
                     HRSGVLEREKVRMIGANADAIDRGEDRNLFKEAMLRIGLDVPRSGIAHTMEEARRVAG
                     DIGSFPLIVRPAFTLGGMGGGVAYNKTEFEEICARGLDLSPVSEILIEESLIGWKEFE
                     MEVMRDRADNCVVICSIENIDPMGVHTGDSITVAPIQTLTDREYQAMRDASFAVIREI
                     GVETGGSNIQFAINPANGRMIVIEMNPRVSRSSALASKATGFPIAKLAAKLAVGYTLD
                     ELRNDITRETPACFEPTIDYVVTKVPRFTFEKFKQADEHLTTSMKSVGEAMAIGRTFK
                     ESLQKALRSLETGRWGWGFDAKAPQAPSAEEITRKLAVPTAERIFWIQTAFSNGFSLD
                     EVQQLTQIDPWFLAQMQDLAKAGDSLDKLDLREAKKLGFSDRQIALARGTTEENIRKE
                     RQEQGIVPGYRLVDTCAAEFEAYTPYFYSTYGDENEARETGRKKILILGGGPNRI"
BASE COUNT         1220 a         1652 c         1435 g         1082 t
ORIGIN      
        1 tgacatggcg caggtcccgc ccggcggcgg ccagcagctc aaccatgttg tccagacagg
       61 cggaatcgct ctggccgggg ggaataaggg gcagcagctt ttgcaggtca tcccccagca
      121 gggtggagga caaatgcggc tcacgcacgc tcaggtggtt caggttcccg cgcagggtgt
      181 tgatttcccc gttatgcgcc agatacctga agggatgggc caggctccag gttgggaacg
      241 tattcgtgct gtagcgctgg tggacaaggg ccagggggga tttgaaatgc tcgctggcca
      301 gatctccgta aaatccttca atctgcgtgc cgaggaacag ccccttgtac acaatgctgc
      361 ggctggagca gctgcataca taacagcctt ccacgcgccg ttccatttcc cggcgcatca
      421 caaacagctt gcgttcaaat tccgcctgat ccgcaaaacc actgccgtca atgaacaact
      481 gacggatcag gggacatgtg cgctgggcgt ttttaccgat gctgtcccgg tccacaggaa
      541 cctgcctcca cgcaatcacg ctgccgccgt tttccgtaac ggcggcttcc aattcctctt
      601 catggccgca tccgccgaac atcatggcca ccccgtattt gcctctggcg ggaagcttgt
      661 tgggaagcgc caggcggaaa aattcatccg gaagggccag cagaatgccc gcgccgtccc
      721 cggtatccgg atcgcttccc acggctccgc ggtgcatgag cctcttgaga acagtaattc
      781 ccatttcaat gattttgtgg cttggttcat ttttcaggtc agccaccagg cccacgccgc
      841 aggcgtccct ctcctgttca aaatcataca atccctgcgg cgcgggaatg cctgtctcaa
      901 attgatcgct attcatcagt ttgctgtttt gtttgggtta agttgactta ttcgtagcaa
      961 aaaccgtgcc aacattgccc agactttcta ttttcccgct ttcaaactgt tttacccccg
     1021 ttttacaaag cctgacgcgg ctgccggcat aacaggacat tttgaagcgg aataaaaata
     1081 tcgttccccc aagaagaatt ttttacattt ttgtaatcac ccttttgttg cgattttaca
     1141 tttttgtaat tcattcttat gcgcagtttt gtttcccttt tttgcagccc cttcgccata
     1201 atattcatat ttcactaata atcaataaat taaaattagc aacatattca tgagaacatt
     1261 tacctgaaaa gttggcacgc ttcttgctaa agaaaaggta caacagttaa ccatgatgag
     1321 tgactcgaca cgaacacaag ccatcagcgc catcgcttcc ctgcccgtgg gcggagctag
     1381 cgaaagcgcc ccggtcagca ttgattatta cggagcggat gtcttcagca cggaagtgat
     1441 gaagaagtac ctgcccaaag atacggcaaa aaccctcctg gccactatcc aggacggact
     1501 tcctctgaac gcggacattg ccgcagatgt ggcccatgcc atgaagcagt gggctctgga
     1561 acggggagcc actcactata cgcactggtt ccagcccatg acgggctcca cggcggagaa
     1621 gcacgattcc ttcctggacc ccaagggaat ggaacccatc atgagcttct ccggcaaaaa
     1681 cctgattgtg agcgaaccgg acgcgtccag tttcccttcc ggcggcctgc gctgcacttt
     1741 tgaggcccgc ggatacaccg cctgggatcc caccagcccc gccttcatca agaggcacgg
     1801 gaacggagcc acgctctgca tccctaccgc ctactgttcc tatacgggag acgcgctgga
     1861 taagaagact ccgctgctgc gctcccgcca ggcattgggc aacgccgtca agaagctgat
     1921 gaagtgcttc ggccttccgg acgaacacgt aaccatcacg ctggggccgg aacaggagta
     1981 cttcctcatt gacaagaatt tttacctgaa ccgcccggac ctggtccaga cggggcgcac
     2041 ccttttcggc gcacctcccg ccaagcacca gcagctggaa gaccattatt tcggctccat
     2101 caaaccccgc gtccttaatt tcatgagcga cgtggaacag gaactctgga gactggggat
     2161 cccggccaag acccgccaca acgaagtggc gcccgcgcaa tttgaactgg cccctctgtt
     2221 tgaggacgtg aacctggcga tcgaccataa catgctggtg atggaggttc tgcgccagca
     2281 ggccagcaag cacggcctgg tatgcctgct tcatgaaaag ccctttgctg gcgtcaacgg
     2341 ttccggcaag cataacaact ggtccatttc ctacgggaac aagaacctgc ttgaccccgg
     2401 ctcggatccg cagcaaaacg ccattttcct gaccgtcctg accgccatca tcgaggccgt
     2461 tgacaaacac agcgacttgc tgcgcaattc cgtggccagc gccgggaacg accaccgcct
     2521 tggcgcgaac gaagcccccc cggccattat ctccattttc ctgggcgccc agctcaatga
     2581 agtaattgag aatatgatca acggctcttc cggctgcggc aagcgcaacg atacgctcaa
     2641 gatcggcgtg gacacgctgc ccgtccttcc ccgggacgcg acggaccgca accgcaccag
     2701 cccgttcgcc ttcaccggca acaagtttga attccgcgca ccgggctcat ctcaatcctg
     2761 cgcgggtccc atgatgaccc tgaacaccat tgtggcggaa tccctcgatt ccctggcgga
     2821 agaactttcc tccttcgctc cggaaacatt cctgccgcag ctccaggaaa cgctcaagcg
     2881 caggatttcc gaacacaaga gaatcatctt caacggggac aattattcgg aagaatgggt
     2941 aaaagaagcg gaacgccgcg gcctccccaa cctgaaaaac acgatgacgg ccctgcacac
     3001 cctggtgaat gaaaagaatg tggccctgtt tgaaaagtac ggcgtctttt ccaggcggga
     3061 gctggaatcc aggtttgaga ttttcctgga agaataccac aggcgcatcc gcattgaagg
     3121 ccgcctgtcc tgggaaatgg ccgccaccat cattctcccc gccctgcgca atgaatacga
     3181 gcaaaccgtt tccgcgctct ccagggcgct ggacgccaag cggaccagcg gcaccgccgc
     3241 cctgcagaag ctggcggaca agctgggcga cgccctggat tccatcgtat ccgacctgga
     3301 taccctggaa acggcgctta cgagctgcca tgaggacatc ctcgttgcga tgtcccaatt
     3361 gagaaccagc gtcgatgccg cagaaaccct ggtgaacgac cggtcctggc ctctccccaa
     3421 gtaccgggaa atgctgttca tctattagag ggtgcaccga cgggctccgg agttatgccc
     3481 ccccccgcgg gtcaagcctc tgcccgcagg ggacggcacg gctccggagc cttggcgcgc
     3541 cctgttccgg cctcctttcc ccgccatgtt caactgatcc ccacaccctg aaaatccgcg
     3601 tcattgaccg taaaaaaact ttcctccgga gctcggcgga cacttaattc ccccggcgga
     3661 aatccgcggt ccaggatcac aggattcttc catcgccatt atgcctaaag acacctccat
     3721 taaaaagatt ctcgtgatcg ggtccggccc gatcgtcatc ggccagggct gcgaattcga
     3781 ctactccggc acccaggcct gcaaagccct ccgggaagaa ggttatgaag tagtgctggt
     3841 gaactccaat cccgccacca tcatgacgga tccggagacc tccccgcgca cttatattga
     3901 accaatcacc ccggagttcg tggagaaaat catcatccgg gaaaaaccgg atgcccttct
     3961 tccgaccttg ggcgggcaga cggccctgaa ctgcgccatg gaactgcatc gcagcggcgt
     4021 gctggagagg gaaaaggtgc gtatgatcgg cgccaacgcg gacgccattg accgcgggga
     4081 ggaccgcaac ctgttcaagg aagccatgct ccgcattggc ctggacgtgc cgcgttccgg
     4141 cattgcgcac accatggagg aagcgcgccg ggtagccggg gacatcggtt cctttcccct
     4201 gattgtccgc cccgccttca cgctgggcgg catgggaggc ggagtagcgt acaacaaaac
     4261 ggaatttgaa gagatatgcg cccgggggct ggatctttcc cccgtttccg aaatcctgat
     4321 tgaagagtcc ctgatcggct ggaaagagtt tgaaatggaa gtaatgaggg accgcgcgga
     4381 caattgcgtg gtcatttgct ccatcgagaa catcgacccc atgggcgtgc ataccgggga
     4441 ttccatcacc gtagccccca tccagaccct cacggaccga gaataccagg ccatgaggga
     4501 cgcttctttt gccgtcatcc gggagatcgg tgtggaaacg ggcggttcca acatccagtt
     4561 cgccattaat ccggccaacg gccgcatgat tgtcattgaa atgaaccccc gcgtttcccg
     4621 ctcctccgcg ctggcttcca aggccacggg gttccccatt gccaaactgg ccgccaaact
     4681 ggccgtgggc tacacgctgg atgaattgcg gaatgacatc acgcgggaaa cgcccgcctg
     4741 ctttgagccc accatcgact atgtggttac caaggttccc cgcttcacgt ttgaaaagtt
     4801 caagcaggcg gacgaacacc tgaccacgtc catgaagtcc gtaggggagg ccatggccat
     4861 cggccgtact ttcaaggaat ccctgcaaaa ggccctgcgc tccctggaaa cgggacgctg
     4921 gggctgggga tttgacgcga aggcgccgca ggcgccttcc gcagaagaaa tcacgcggaa
     4981 actggctgtt cccacggcag aacgcatttt ctggattcaa acggcgttca gcaacggatt
     5041 ttccctggat gaagtgcagc agcttaccca gattgatccc tggttcctgg cacaaatgca
     5101 ggacctggcc aaggcgggag acagcctgga caagctggat ctgcgggaag ccaagaagct
     5161 gggcttttcc gacaggcaga tcgccctggc ccggggaacc acggaagaaa acatccggaa
     5221 ggaacgccag gaacagggca ttgtgcccgg atatcggctg gtggatacct gcgccgcgga
     5281 gtttgaagcc tacaccccct acttttattc cacctatgga gatgaaaatg aagcccgcga
     5341 aacaggcaga aaaaaaatcc tcatcctggg cggcggcccg aaccgcatc
//
LOCUS       DAOMSU010000143         5383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1347_length_5383_cov_2.825263, whole genome shotgun sequence.
ACCESSION   DAOMSU010000143 DAOMSU010000000
VERSION     DAOMSU010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1347_length_5383_cov_2.825263"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2048
                     /locus_tag="PK077_06910"
     CDS             <1..2048
                     /locus_tag="PK077_06910"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HRN24069.1"
                     /translation="VKTYYAQKPDFQGQEERLDKEIERIKKLASSLEEVNTDTLSQAS
                     LVQRDEALYACQELEESLQSEKEQISYLKELFSSLNGKIDALNNESAKLFSALFNRVF
                     YTPSHAARYIFLKPHKTYETCMGAWETSADSKLQLPSDPETLKHYGLILLGIILCSYC
                     LSRLIIFLGFRNTLKKTGHYNKRHAFANVTTILIVALILVIASLRTPNYLLESAASLG
                     AEFLLLVSAILFSLVTRLPYGTINSGIRIYMPYLLLGGFFMLLRMFMAPNIIVDISMP
                     ILFTLVSLFSLLTWVRQRHKLPRLDRFFSTVSLVFTILGSVLCWTGFSFMAFLVLMTW
                     IMLMTSILLLVGLWDLLHHYENRRKERNKRAILWFRPFISKLLLPCLTIFLILFSIIW
                     PAATFDMGDLIINKIFATTEIKDLLTFSWSSVITVILMAIVLNYLIFLGKNTLHEIYG
                     EDYEVGTIPTFVTLSTLFLWGLFVFTALIIMNANYNGLLMVMGGLSMGIGFALKDTIE
                     NIISGLSLMLGRLRQGDMIECDGYRGRVSSLGYRSTMIETLDGSIIAFQNSQLFNKNF
                     RNMTRNHKFECVKVEVGISYGTDVERARKIILETLATLPFLSKVKKTSVVLDSFGDSA
                     VNLGVWVWVPVMTKSSSLSSVREHIYNAFNEHGISIPFPQQDLYVKEFPGGAPVQGT"
     gene            2074..2484
                     /gene="queF"
                     /locus_tag="PK077_06915"
     CDS             2074..2484
                     /gene="queF"
                     /locus_tag="PK077_06915"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HRN24070.1"
                     /translation="MSDDHLTLLGSQSSFFTNPDDARLESFPNRGTRPYTITLDTHEF
                     SSLCPVTGQPDSCHLTITYVPAEKCVETKSLKYYLASYRNYPAFNEQIVNRITDDLVA
                     AISPRWLKVEGRFSPRGGIQLTATAEHNPENRPL"
     gene            2493..3164
                     /gene="queC"
                     /locus_tag="PK077_06920"
     CDS             2493..3164
                     /gene="queC"
                     /locus_tag="PK077_06920"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HRN24071.1"
                     /translation="MDKMETAVLLSGGLDSSVALHWAHRNHRVQVALSFDYASNHAER
                     ELKCAAWQAASLGIEHRVIDISSISSHLKSALLSGADDIPDGSYDEELMKQTVVPFRN
                     GIFLSIAAGVAESCGAQQLVIAAHSGDHSIYPDCREEFMAAMTEAIRLGTYAGLGILR
                     PFIHMSKGGIAAMGQDLGVDFSRTYSCYKGGAVHCGVCSTCMERREAFREAGIPDPTA
                     YAPAE"
     gene            complement(3228..3848)
                     /gene="tmk"
                     /locus_tag="PK077_06925"
     CDS             complement(3228..3848)
                     /gene="tmk"
                     /locus_tag="PK077_06925"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRN24072.1"
                     /translation="MSFTGERKGRLIVFEGIDGTGKSTHIGHLRKYLEGKGLEVVQSF
                     EPTRGRWGRMLRDSAVTGRLSVEEEVALFLKDRREHVETLIAPALARGAWVLLDRYYL
                     SMMAYQGARGIDPEVIRAANEEFAPVPDAVVWLDIPVSVALERIGNRGERDAFETEAG
                     LAACRSVFASVHAPWMLRIDADAGKEEVAARVRKALSMRFPDVIGA"
     gene            complement(3845..5215)
                     /locus_tag="PK077_06930"
     CDS             complement(3845..5215)
                     /locus_tag="PK077_06930"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRN24073.1"
                     /translation="MISFTNISGAEEIGANCYLLEMDGTRIVLDSGMHPKKEGKAAMP
                     DFDSLEPNSVEAVFLSHSHLDHLGTLPVLQEKQPAAEVFMTPAAAALSEVMLHNSVNV
                     MSAKRLDLGIVEYPFFTHNDLDRLSDAWHAKSCNEVFRVGFRQNVLATFYDAGHILGS
                     AGVMLEGESGHTVFYTGDVQFEDQSMIPGADFPESGVDTLVMECTRGGFQRSAHYSRP
                     EEMVRFGKAIAETLERGGAVLIPVFAIGKSQEMLFNIHRFKQQGVIPANTPVYFGGLS
                     AKVSLLYDRFAGLTRRHDHEFKLKEEIQTVPLPRKGKAPLVCSPGNIYVVSSGMMTEN
                     TLSNVMAEQVLPQEKNAILFVGYADPDSPAGLLKATPEGELVKMRPNGQPVRRKCTVD
                     CFDFSGHATRDSLVNYAVKLNPRQVVLVHGDPDAVEWMHHTLSAKMPDSTIVVPEPGR
                     RYTFEP"
BASE COUNT         1188 a         1726 c         1346 g         1123 t
ORIGIN      
        1 tggtgaagac gtattacgcc caaaaaccgg atttccaggg gcaggaggaa cggctggaca
       61 aggaaataga acgcattaaa aagctggcgt catccctgga ggaagtaaat acggacaccc
      121 tgtcgcaagc ctccctggtt cagcgggacg aagccctgta tgcctgccag gagctggaag
      181 aatccctcca aagtgaaaag gagcaaatca gctacctcaa ggaacttttc agcagcctga
      241 acggcaaaat agacgcgctc aacaatgaaa gcgccaaact gttctccgcc ctgtttaaca
      301 gggtcttcta cacgccgtcc cacgcggccc ggtacatctt tctcaaaccg cacaagacgt
      361 atgaaacgtg catgggggca tgggaaacat ccgcggacag caagctccag cttccgtcag
      421 acccggaaac gctcaagcat tacggactta tcctgctggg aatcatcctg tgctcctact
      481 gcctctcccg cctcatcatc ttcctgggat tccgaaatac attgaaaaag acggggcatt
      541 acaacaaacg ccatgctttc gccaacgtca ccaccattct tatcgtggct ctgatcctcg
      601 tcattgcctc actgcgcacg ccgaactacc tgctggagag cgcggcctcc ctgggcgcgg
      661 aattccttct tctggtcagc gccatcctgt tctccctggt cacgcgcctt ccgtatggca
      721 ccattaactc cggcatccgc atctacatgc cctatctact tctgggcgga ttcttcatgc
      781 ttctgcgcat gttcatggcc cccaacatca tcgtggacat ttccatgccc atcctgttca
      841 cgctggtttc cctcttctcc ctgctgacct gggtgcggca gaggcacaag ctgccccggc
      901 tggaccgttt tttctccacc gtatctctgg tattcaccat cctgggcagc gtcctgtgct
      961 ggactggatt cagcttcatg gccttcctgg tgctgatgac ctggatcatg ctgatgacca
     1021 gcatcctgct gctggtgggg ctgtgggatc tgctccacca ttatgaaaac cgccggaagg
     1081 aacggaacaa gagggccatc ctgtggttcc gccccttcat ctccaagctg ctgcttccct
     1141 gcctgaccat tttcctcatc ctgttcagca tcatctggcc cgccgccacc ttcgacatgg
     1201 gggacctcat cattaataaa attttcgcaa ccacggaaat caaggatctg ctcactttca
     1261 gctggagcag cgtcatcacg gtcatcctga tggccattgt tctcaactac ttgatattct
     1321 tggggaaaaa tacgctgcat gaaatctatg gagaagatta tgaagtaggc accatcccca
     1381 ccttcgtgac actctccacc ctttttctct gggggctctt cgtttttacg gcgcttatca
     1441 tcatgaacgc caactacaac gggctgctga tggtcatggg aggcttgagc atgggcatcg
     1501 gcttcgccct gaaagatacc attgaaaaca tcatcagcgg cctctcccta atgctgggtc
     1561 gcctgcgcca gggcgacatg attgaatgcg acggctaccg gggccgcgtc tcctccctgg
     1621 gctaccgctc caccatgatc gagacgctgg acggctctat catcgccttc cagaactccc
     1681 agcttttcaa caagaacttc cgcaacatga cccgcaacca caaattcgaa tgcgtgaagg
     1741 tggaagtggg catttcctac gggacggacg tggaaagggc gcgcaaaatc attctggaaa
     1801 cgctggcaac cctgccattc ctttccaaag tcaagaaaac cagcgtggtg ctggacagtt
     1861 tcggagacag tgccgtcaat ctgggcgtat gggtctgggt tcccgtcatg acaaagtcct
     1921 ccagcctttc ctccgtgcgg gaacacatct ataatgcgtt caacgaacac ggcatttcca
     1981 tcccattccc tcaacaggac ctgtacgtga aagaattccc cggcggagcc cccgtccagg
     2041 ggacctgaac atccattacc atatacaaca accatgtccg acgaccatct gactctatta
     2101 ggctctcaaa gctccttctt taccaatccc gacgacgcca ggctggaatc cttccccaac
     2161 cgcggcacgc gtccctacac catcacgctg gacacccatg aattttcctc cctctgcccc
     2221 gttaccggtc aaccggattc ctgtcacctg acgatcacct acgttccggc ggaaaagtgc
     2281 gtggaaacca aatctctcaa atactacctg gcctcctacc gcaactatcc ggcttttaat
     2341 gaacaaatcg tcaaccgcat cacggatgat ctggtggccg ccatttcccc gcggtggctg
     2401 aaggtggaag gccgcttctc cccccgcggc ggcatccagc tgaccgcaac agccgaacac
     2461 aatccggaaa accgccccct gtaatccaga ccatggacaa aatggaaaca gccgtactgc
     2521 tcagcggggg attggactcc agcgtagccc tgcactgggc ccaccggaac caccgcgtcc
     2581 aggtggccct cagctttgac tatgcctcca accacgcgga acgagagctg aaatgcgctg
     2641 cctggcaggc cgccagtctg ggcatagaac accgcgtgat tgacatttcc tccatctcct
     2701 cccatttgaa atcagccctc ctctccggcg cggacgatat tccggacggt tcctatgatg
     2761 aagaactgat gaagcagacc gtcgtcccct tccgcaacgg catcttcctg tcaatcgccg
     2821 ccggagttgc ggaaagctgc ggggcgcagc agctcgtcat tgcggcccat tccggggacc
     2881 atagcatcta tccggactgc cgggaggaat tcatggccgc catgacggaa gccattcgcc
     2941 tgggaaccta tgccggtctg ggcatcctgc gccctttcat ccacatgtcc aagggcggca
     3001 tcgccgccat gggccaggat ttgggcgtgg acttttcccg cacctattcc tgctacaagg
     3061 gcggagccgt ccactgcggc gtctgttcca cctgcatgga acgccgggaa gccttcaggg
     3121 aagccggaat tccggatcca acggcttatg cgcctgcgga atagcatcca aatgccagga
     3181 atgaaacgct aaggaaaaat ccccggccta tggccgaaag gagatgccta tgccccaatg
     3241 acatcgggga aacgcatgga aagagccttc cgcactcttg ccgccacctc ttccttcccg
     3301 gcgtccgcgt ctatgcggag catccaggga gcatggacgg atgcaaacac gctgcggcag
     3361 gccgccaggc ccgcttccgt ctcaaaagcg tcgcgttccc cgcggttccc aatgcgttcc
     3421 agcgccacgg aaacgggaat gtccagccag accacggcat ccggcaccgg ggcaaattct
     3481 tcattggcgg cacggataac ctccggatct atgccgcgcg cgccctggta agccatcatg
     3541 gaaagatagt agcgatccag cagaacccat gccccccgcg ccaatgcggg agcaatcagc
     3601 gtttccacat gttccctcct gtctttcagg aacagggcta cttcctcctc tacggaaagg
     3661 cgtccggtca cggcggagtc ccggagcatc cggccccacc gtccgcgtgt gggctcaaaa
     3721 ctctgcacca cttccagccc cttcccctcc aaatacttcc tcaagtggcc gatgtgggtg
     3781 gacttgcccg tgccgtcaat gccttcaaag acgatcagcc tcccctttct ctctccggta
     3841 aaactcatgg ctcgaaagtg tagcggcgtc ccggctcagg aacgacgatg gtggaatcag
     3901 gcattttggc ggatagcgtg tggtgcatcc actccacggc atccgggtcc ccgtgcacca
     3961 gaaccacctg cctggggttc agcttcacgg cataattaac cagggagtcc cgggtggcat
     4021 gcccggaaaa atcaaaacag tccacggtgc acttgcggcg caccggctgc ccgttgggcc
     4081 tcattttcac cagttcccct tcaggagttg ccttcagcag tcccgccgga gaatcgggat
     4141 ccgcatatcc tacgaacaaa atagcattct tctcctgggg aagaacctgc tccgccatga
     4201 cgttggaaag cgtattttcc gtcatcatgc cgctggacac cacataaata ttccccgggg
     4261 aacacaccag cggagctttt cccttgcgcg gaaggggcac ggtctgaatc tcctccttca
     4321 gcttgaattc atggtcatgc ctgcgcgtca acccggcaaa acggtcgtac aacagggaaa
     4381 ctttcgcgct cagcccgccg aagtacacgg gagtattggc gggaatcacc ccctgctgct
     4441 tgaaacggtg aatgttgaac agcatctcct ggctcttgcc gatggcgaat accggaatca
     4501 gtacggcgcc gccgcgttcc agcgtctccg caatcgcttt cccgaacctc accatctctt
     4561 ccggccggga atagtgggcg ctgcgctgga aaccgccgcg cgtgcactcc ataaccagcg
     4621 tatctacgcc ggattccgga aaatcagccc cgggaatcat gctctggtct tcaaactgca
     4681 cgtcccccgt ataaaatacg gtatggccgc tctcaccctc cagcatcacg ccggcagacc
     4741 ccagtatgtg cccggcatcg taaaacgtgg ccagcacgtt ctgacggaag cccacgcgaa
     4801 acacttcatt gcaggacttg gcatgccagg cgtcgctcaa tctgtccaga tcattatggg
     4861 tgaaaaaagg atactccaca atgcccaggt ccagcctctt ggcgctcatc acattcacgg
     4921 aattatgcaa catcacttca gacagggctg ccgcggcggg cgtcatgaac acctccgccg
     4981 ctggctgttt ctcctgaagc acaggcagcg ttcccagatg gtccaggtgg gaatggctta
     5041 aaaaaactgc ttccacggaa ttgggttcca gggaatcaaa atccggcatg gccgccttcc
     5101 cctccttctt ggggtgcatc ccgctgtcaa gcacaatcct ggtgccgtcc atctcaagca
     5161 aatagcaatt ggccccaatt tcctcggccc cgctgatatt ggtaaaactg atcattatat
     5221 gccgcaggag agtaccccgt gcctcccgcc gtgtcaatgc cgcaagccca agcgttcccc
     5281 ttccgggaag ggaacgcagg ccaatgggga agaagccctc ctctaccctg cggaacgttt
     5341 ttgcggctgg gggaaaacag gctcggaaaa cggcaggggc aaa
//
LOCUS       DAOMSU010000144         5372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1353_length_5372_cov_2.484672, whole genome shotgun sequence.
ACCESSION   DAOMSU010000144 DAOMSU010000000
VERSION     DAOMSU010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1353_length_5372_cov_2.484672"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(312..824)
                     /locus_tag="PK077_06935"
     CDS             complement(312..824)
                     /locus_tag="PK077_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24074.1"
                     /translation="MKKSILAVCLGLAGFWSVLAEDSPIAAPGRVNEQGDAELPSGEL
                     LSDHVAEAEFTGIIHRKCRFLTSLCPDKCDHARDFAVFRIVKYLDYRKPGKYGDEKQE
                     QLMVDVNPAHKPILQSADILKKISALKPGDKVVLHWAHYYMHRNSSSFPERPVISVEP
                     AALSGGKTGK"
     gene            1012..2946
                     /locus_tag="PK077_06940"
     CDS             1012..2946
                     /locus_tag="PK077_06940"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HRN24075.1"
                     /translation="MDNNRTSTVPIVPKQYRLPFFMLVSCFALWGLLNNMTDNLVPAF
                     SKIFMINASESAGVQISFYGAYAVLAIFASILLEEFSYKAGVLIGLGLYMIGALCYIP
                     AAIGQSFDIYLMAIFVLAGGLSILETTCNPFVLSMGSQETSVRRLNFAQAFNPIGSLT
                     GIFLAKYFILAHLNDADMDTRKAMDPEQLNAIVSDELFWVCVPYVGLVAIAAVIWFFF
                     CRYKSDDENVPTRENTQPLSSKLGRLGLCIALILIPFLFQYYSAFEFSMVQQILIMMI
                     GPIAFLLIMPDYRCQLMKLVKLPRYFCGVAAQFFYVGVQIAVWTWTIKYIMSVFPGMQ
                     EAAASNYYIFSIILFIACRAITTALMKKFNPANMMSLFAVAGILCCLGTIYLPREISV
                     WTLVGISGCMSLMFPTIYGIALRGLGSEVKLGAAGLIMAILGGAVITPYMGSWIDNTT
                     VKNQVAYFESINRPLIAQAEAADARKSLSGAMLALYRMSEASPADGEEQARAAGKFQK
                     NLETFFTLPSLEGDAFEQAAKSLPGCSSLKPRELAVFKANLERLPAPAGREQQIQQFS
                     LIPALSTLSGNQAEVLDNLIALPLDMKQFQTAQENFVEKAVRSSFFIPIICFAVVLIY
                     GFFFRNTHLKKEEEEKPAQA"
     gene            2974..4752
                     /locus_tag="PK077_06945"
     CDS             2974..4752
                     /locus_tag="PK077_06945"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HRN24076.1"
                     /translation="MKTALPTIGIRPTIDGRRLGVRESLEDQTMNMAKAAAALIEANI
                     RHASGEPVKCVIADTCIGGPAEAAACADKFKANNVGVSLAVTPCWCYGSETFDMDPFT
                     PKAIWGFNGTERPGAVYLAAALAGLNQKGFPAFSIYGKDVQDATDTSIPEDVAEKILR
                     FCRAGLALATLRGKGYLSIGGCSMGIAGSIVDQSFWENYLGIRVQAVDMTEVRRRMDQ
                     KIYDEAEFDLAMQWADSVFKFAEDKNRPDLKLDEAGRRKTFKESVLMAMIFRDMMQGN
                     PKLKKIDREEESLGYQAIAGGFQGQRHWTDFYPNGDIAETLLNSTFDWNGPRAPLPFA
                     TENDSLNGACLLFGYLLTGQANVFADVRTYWSPEAVKRVTGYTLEGHAKDGIIHLINS
                     GSAALDGCMACTGKDGQPVMKKHWDVTPDDAKTMMEQAVWCPANREYFRGGGYSVHYV
                     TRGGAPVTMMRLNIVKGLGPVLIVAEGWSVDLPAEVHKTLDERTDPGWPTTWFAPRLT
                     GKGAFTDVYSVMANMGANHGAFTYGHIGADILTLASMLRIPVYAHNIPDDQIYRPSAW
                     GLHGTADPEGADFRACANYGPLYGKY"
     gene            4872..5168
                     /locus_tag="PK077_06950"
     CDS             4872..5168
                     /locus_tag="PK077_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRN24077.1"
                     /translation="MKKQDVERELLSQLLRSGAGIAANLSEAVYGSRRRDFFSNPHIA
                     LKEYSGTHARPKLLRATGSLTETESSSVNQDSTEIPKMLISINKPTQQAQPDSY"
     gene            5193..>5372
                     /locus_tag="PK077_06955"
     CDS             5193..>5372
                     /locus_tag="PK077_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRN24078.1"
                     /translation="MYSLCLDCGATNVRAMVVDKQGAIVGKASQPNATLPGEENPEFH
                     VWDADRIFRQLSECAV"
BASE COUNT         1216 a         1655 c         1339 g         1162 t
ORIGIN      
        1 cgccacaccg ttccggcagg acataaggcc gtatttggtg gaaagcgttg taaacttgcc
       61 acgcctggcc tgctcaaagc tttccttgcc gagaacggag ggaatagaca ggcccagtgc
      121 gcacaacagg ccaagcccca ttaatttatt caaagataaa cgcatcgtat ttatggtgtg
      181 tttcgcggga tatatacgcc gccccccgcg gcaatcttgc ccggagcagc gttatttgcc
      241 ggtccgtttc ctaacacacg gctttccgtt gcgtccagcc ccgcttgaac ggcctgagga
      301 aagaccagtc cttactttcc cgtttttcct ccggaaagag ccgccggctc cacggaaatc
      361 acgggacgtt ccgggaaaga actggaattc cggtgcatat aataatgagc ccagtgaaga
      421 accaccttgt cccccggttt aagggcggat atcttcttca aaatatccgc actctgcagg
      481 atgggcttgt gcgccggatt aacgtcaacc atcagctgct cctgcttttc atcgccgtac
      541 ttgcctggtt tccggtaatc caggtacttg acgatccgga aaacggcgaa atccctggca
      601 tggtcgcact tgtccgggca cagggaagta aggaacctgc atttccgatg aattattccc
      661 gtaaattccg cttccgccac atgatccgac aacaattccc cggaaggcag ttccgcatcc
      721 ccctgttcat ttacccttcc cggggccgca atgggagaat cttcagccag aacgctccag
      781 aatccggcca ggcccagaca gacggctaga atactcttct tcatctcttc attcctatcc
      841 gccgcggaaa cacaagtcaa gaagaagaaa gagcttctgc ggacactgac ggaatatcac
      901 catggaaaaa catcatttta tttttttatc aacaggttgg ataatttctg gactcttttc
      961 ggtttccaga ttgacgcacc gccgtccttg tgagaaattg acaaaagcaa tatggataac
     1021 aaccgtacct ctaccgtgcc gatcgttccc aaacaatacc ggctgccgtt cttcatgctg
     1081 gtctcctgtt ttgcgctgtg gggcctcctg aacaacatga cggacaacct ggttccggca
     1141 ttcagcaaaa tattcatgat caatgcttcg gaatccgccg gagttcagat ttccttctat
     1201 ggggcctatg cggtgcttgc cattttcgcc tccatccttc tggaggaatt ttcctataag
     1261 gcgggcgtcc tcatcgggct ggggctctac atgatcggcg cgctgtgcta cattcccgcc
     1321 gccatcgggc aaagtttcga tatctatctg atggctatct tcgtgctggc cggaggcctc
     1381 tccattttgg agaccacctg caaccccttc gttctctcca tggggtccca ggaaacgagc
     1441 gtccgccgtc tgaacttcgc ccaggccttc aatcccatag gctccctgac aggtattttc
     1501 ctggccaaat atttcattct ggcacacttg aacgatgcgg acatggacac ccgcaaagcc
     1561 atggacccgg aacaactgaa cgccattgtc agcgatgaac ttttctgggt atgcgttcct
     1621 tatgtgggcc tggtagccat cgccgccgtc atctggttct ttttctgccg ttacaaatcc
     1681 gacgatgaaa acgtacccac ccgggaaaac acacagcccc tttcctctaa actcggccgc
     1741 ctgggactct gcatcgccct gattctcatc cccttcctgt tccagtacta ttccgctttc
     1801 gaattcagca tggtgcagca aatcctgatt atgatgatcg ggcccatcgc cttcctgctc
     1861 atcatgccgg actaccgctg ccagcttatg aaactggtga agcttccgcg ctacttctgc
     1921 ggcgtggcag cccagttttt ctatgtaggc gtgcagattg ccgtgtggac gtggacgatt
     1981 aaatacatca tgtccgtctt ccccggaatg caggaagcgg cggcctccaa ctactatatc
     2041 ttttccatca tcctgttcat cgcctgccgc gccatcacta cggccctgat gaaaaaattt
     2101 aacccggcca acatgatgtc ccttttcgcc gtcgccggca ttctctgctg cctgggcacc
     2161 atctatctgc ccagggaaat ctccgtctgg acactggtcg gaatctccgg ctgcatgtcc
     2221 ctgatgttcc ccactattta cggcatcgcc ctgcgcggac tggggtctga agtgaaactg
     2281 ggagctgcgg gccttatcat ggccattctg ggcggcgcag tcattacccc gtacatgggt
     2341 tcctggatag acaacaccac agtaaaaaac caggtggcct attttgaatc catcaaccgt
     2401 cctctgatcg cgcaggcgga agctgcggac gctcggaaat ccttatctgg agccatgctg
     2461 gcgctgtaca ggatgtcaga agccagccct gcggacggag aagaacaagc tagggccgcc
     2521 ggcaaattcc agaaaaacct ggaaaccttc tttaccctcc cctccttgga aggggacgct
     2581 tttgagcagg cggccaaatc cctgcccggc tgctcctccc tcaagcctag ggaactggcc
     2641 gttttcaaag ccaatctgga acgcctcccc gctcctgccg gaagagaaca gcaaattcag
     2701 caattctccc tgattccggc gctctccacg ttatccggaa accaggcgga agtgctggac
     2761 aacctcatcg ccctgcccct ggacatgaag caattccaga cggcccagga aaacttcgtg
     2821 gaaaaagcgg tgcggtcatc cttctttatt cccatcatct gctttgccgt ggtattaatt
     2881 tacggcttct tcttccgcaa cactcatctg aaaaaggaag aggaagaaaa gcctgcccag
     2941 gcatgaacca gcttcacatc acctacgcaa gatatgaaaa cagcattacc aaccatcggt
     3001 atccgcccca cgattgacgg tcgccgcctc ggcgtccggg aatccctgga agatcaaacc
     3061 atgaacatgg ccaaggcggc cgccgccctt attgaggcca acatccgcca cgcctccgga
     3121 gaacccgtca aatgcgtgat tgcggacacc tgcatcggcg gcccggcgga agccgccgcc
     3181 tgcgcggaca aattcaaggc caacaacgtc ggcgtctccc tggccgttac gccctgctgg
     3241 tgctacggca gtgaaacatt tgacatggat ccgttcactc ccaaggccat ctggggcttc
     3301 aacggcacgg aacgccccgg tgccgtgtat ctggccgctg cgctggccgg cctgaaccag
     3361 aaaggattcc cggctttctc catttacgga aaagacgtgc aggacgccac ggatacttcc
     3421 atcccggaag acgtagctga aaaaatcctg cgcttctgcc gcgccggcct cgccttagcc
     3481 accctccgcg gaaaaggcta tctttccatc ggcggctgct ccatgggcat tgccggttcc
     3541 attgtggacc agtctttctg ggaaaattac ctgggcatcc gcgtacaggc cgtggacatg
     3601 acggaagtgc gccgccgcat ggaccagaaa atttatgacg aagcggaatt cgacctcgcc
     3661 atgcaatggg cggattccgt ctttaaattc gccgaggaca aaaaccgtcc ggacctgaag
     3721 ctggatgaag ccggacgccg caaaaccttc aaggaaagcg tgctgatggc catgatcttc
     3781 cgcgacatga tgcagggcaa tcccaagctc aagaaaatcg accgtgaaga agaatccctg
     3841 ggttaccagg ccattgccgg cggcttccag ggccagcgcc actggacgga cttctacccg
     3901 aacggagaca tcgcggaaac cctgcttaac tccacctttg actggaacgg cccacgcgcc
     3961 ccgcttccct tcgccacaga aaatgactct ctcaacggcg cctgcctcct cttcggctat
     4021 ctgctgaccg gacaggccaa tgtcttcgcg gacgttcgca cctactggag cccggaagcc
     4081 gtaaagcgcg tcacgggtta tacgctggaa ggccatgcca aggacggcat catccacctc
     4141 atcaattccg gctccgccgc cctggacggc tgcatggcct gcacgggcaa ggacggacag
     4201 cccgtcatga aaaaacactg ggacgtcacc ccggacgatg cgaaaaccat gatggaacaa
     4261 gccgtctggt gcccggccaa ccgggaatac ttccgcggcg gcggctactc cgtccactac
     4321 gtcaccaggg gcggcgctcc cgtcaccatg atgcgcctga acatcgtcaa gggactcggc
     4381 cccgtcctca tcgtggcgga aggctggtct gttgatctgc ctgccgaggt gcacaagacc
     4441 ctggacgaac gcactgaccc gggctggccg accacctggt tcgccccgcg cctgaccggc
     4501 aagggagcct ttacggacgt gtacagcgtc atggccaaca tgggagccaa ccacggagcc
     4561 tttacttacg gccacatcgg cgcagacatc cttacgctgg cctccatgct gcgcattccc
     4621 gtatacgccc ataacattcc ggatgaccaa atctaccgcc cctctgcctg gggtctccac
     4681 ggtacggcgg atccggaagg agctgatttc cgggcctgcg ccaactacgg gccattatac
     4741 ggaaaatact gaaaacgaaa ggcaacagtt aataggtaat agtaaacagc ttcggataaa
     4801 acatggcaga aagccccgtt tccgcgaaaa gcttttcttt cgcggtccgc attattcaat
     4861 gatgaaggca cttgaaaaag caggacgtcg agcgggaatt gctcagccag ctcctacgtt
     4921 ccggtgcggg cattgccgcc aatttatccg aagctgttta cgggagccgc agaagggact
     4981 tcttttccaa cccccacatc gccctgaagg aatattccgg gacgcatgca cggcctaaac
     5041 tgctgcgcgc cacgggaagc ctgacggaaa cggaatcttc ctctgtcaac caggacagca
     5101 cagaaatccc taaaatgctc atttctatta acaaaccaac tcaacaagct cagccggatt
     5161 cctattaact tttcactatt aaatcttaac tcatgtactc cctctgcttg gactgcggcg
     5221 cgactaatgt gcgcgccatg gttgtggata aacaaggcgc cattgtgggg aaggcctcac
     5281 agccgaacgc cacgctgccc ggtgaggaaa atccggaatt tcacgtctgg gacgccgacc
     5341 ggattttcag acagctctcc gaatgtgccg tg
//
LOCUS       DAOMSU010000145         5368 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1354_length_5368_cov_2.975155, whole genome shotgun sequence.
ACCESSION   DAOMSU010000145 DAOMSU010000000
VERSION     DAOMSU010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5368)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5368
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1354_length_5368_cov_2.975155"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PK077_06960"
     CDS             <1..516
                     /locus_tag="PK077_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24079.1"
                     /translation="TYSVCSRKVFEKRYQAALWAWPLQTPFPRLYETFSSELAYDARG
                     NPVGNTNNIMAVSDAELDAALDAERNAPDTGSLKLTLHRAQQRLYELCVWIPGWREPY
                     THVACWRWIRWPESPTRFCSPRIYNPLESHLYWVDEEMKKETLEARSRGVPFEEKHQI
                     IYLERQDGAVP"
     gene            615..2957
                     /locus_tag="PK077_06965"
     CDS             615..2957
                     /locus_tag="PK077_06965"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRN24080.1"
                     /translation="MKLSFFSVLLLAGHLCVAAPMPLPESNDGARHVFSTNQENFLMD
                     GKPIKIISGEMHYPRVPRQHWKDRFQRIKAMGMNTVCTYLFWNVHEPEPGKWDFSGNL
                     DFVEFIKEAQKAGLWVIVRPGPYVCAEWEFGGFPGWLLKDEDLKVRSQDPRFLEPAMA
                     YLKKVCSMLEPLQITKGGPIIMAQVENEYGSYGSDKDYVKKHLDVIRKELPGVVPFTS
                     DGPNDWMIKNGTLPGVVPAMNFGGGAKGAFANLEKHKGKTPRINGEFWVGWFDHWGKP
                     KNGGSTEGFNRDLKWMLENKVSPNLFMVHGGTSFGFMNGANWEGAYTPDVTNYDYGAP
                     ISENGTLTDRYRTFRQTIQDYYGDTYKLPEPPAQPEMMELPPITFTETAGMFSRLPQP
                     SIRKEPVHMEALGQSLGFILYRTKVQGPVKGELKMNNMQDRAIVYVDGKRQGAADRRY
                     KQDSCDVVIPSGLHTVDIFVENMGRINFGGQIQGERKGIRGPITLDGKKLENFLIYNF
                     PCKGVELLPFSGKKPAGDQPVFHRGYFNVSNPKDTYLDMRDGWKKGVVWVNGRNLGRF
                     WFIGSQQALYCPGEYLKPGKNEIVVLDVDGGFGTVKGVKEAIYEVNRDPAMADVFRVG
                     KPVAPAAGQLVHKGSFAKGADQQEIKFRAPVQARYIAIVSKNAHDNGPHAAIAELNFL
                     DASGNLLPREQWSVVYADSHETTGEAAQAGLVMDNQPTTYWHTKWQGDNPQHPHMIVL
                     DLGKVQKLSGFRYLPRQNRENGRIKDYEVYASPKPFKPAK"
     gene            complement(3210..3812)
                     /locus_tag="PK077_06970"
     CDS             complement(3210..3812)
                     /locus_tag="PK077_06970"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HRN24081.1"
                     /translation="MNKTILLGLALAASVSSASAYHADKYDGQFGMSLEGAYGIATKD
                     AMPNVAGGNLSIFNYIETGSIVHQISLNGGILAGSHHPSVQDLGISGPYTASLRSTYI
                     PMMAGYTLNLPIGDYTMFYLGGKVGATYGDVKTTIHGLEDGSRSRTISSTKFSWAAQA
                     GFKFSISNSADFVLGYEYYQIQGYNDPGYHTIKLGFSWNF"
     gene            3960..5282
                     /locus_tag="PK077_06975"
     CDS             3960..5282
                     /locus_tag="PK077_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HRN24082.1"
                     /translation="MEQQACDLLVTAFRMLGGAEQSSAAGDAAVAVRDGKILETGSAA
                     ELEARWTPSVRRDLGNALLMPGLINAHTHVPMTFLRGFADDLPLMEWLTGHIFPVEAR
                     LTDKIVSLGARLGMYEMMRTGTTAFVDSYLLEANVLQEAERMGMRCVGGEVVFAFPSP
                     AYGGWDGAEALYREQAERFSGRGRVQVALMPHSVYTTSDEVLRRSMKLAEELDMMLHI
                     HLSESAGEVEQCRSLHGGRRPVGYARDMGLLNERAVLAHMVDVTDEELELVAASGAAV
                     VHNPVSNLKLASGFARVRDMVQAGVPVSLGTDGACSNNSLDMFETMKLAAILAKGYSG
                     DATALPAMQALKMATAEGARIFRTPGLGTLVPGAPADMIALNLDEPNLCPMFNETSHA
                     VYASSGKDCVFTMVEGRILYDHGIYTDGLYADTAAEMRDLVKWVKNSD"
BASE COUNT         1183 a         1409 c         1604 g         1172 t
ORIGIN      
        1 acctacagtg tctgttcccg taaggttttt gaaaaacggt atcaggcggc tctgtgggcg
       61 tggccgctgc agacgccgtt cccacggctg tatgaaacat tttcttccga actggcgtat
      121 gatgcccgcg gaaatcccgt aggcaatacg aataatatta tggctgtgtc ggacgctgaa
      181 ctggatgccg ccctggatgc ggaacgcaat gccccggata ccggttccct gaagctgact
      241 cttcaccgtg cccagcagcg tctttatgaa ctttgcgtct ggattcccgg ctggagggaa
      301 ccatacacgc atgtcgcctg ctggcgctgg atacgctggc cggaatctcc cacccggttc
      361 tgttccccca gaatttacaa tccgctggaa tcgcacctgt actgggtgga tgaagaaatg
      421 aagaaggaaa cgctggaggc tcggagccgt ggcgtaccgt ttgaagaaaa gcatcaaata
      481 atatatcttg aaagacagga tggtgccgtt ccgtaataaa accgggtcac cgtctccttt
      541 ttaaattgaa aggaggtgat ttttcatcat aatctccttc gcatttaccg taattaatta
      601 ttaattccat aatcatgaaa ttatcctttt tctccgttct gcttctggca gggcatcttt
      661 gcgtggctgc tcccatgcct ctgccggaat ccaatgacgg agccaggcat gtcttctcta
      721 ctaatcagga aaattttttg atggacggaa agcccatcaa aatcatttcc ggggaaatgc
      781 attatcctcg cgtgccgcgc caacactgga aggacaggtt ccagcgcata aaggccatgg
      841 gcatgaatac cgtctgcact tatctgttct ggaacgtgca cgaaccggaa cccggcaaat
      901 gggacttctc cggcaatctg gattttgtgg aattcatcaa ggaagcgcag aaggccggcc
      961 tgtgggtcat tgtgcgtccc gggccctatg tgtgcgcgga atgggaattc ggcggatttc
     1021 ccggctggct gctgaaggat gaagatttga aagtccgttc ccaggatccc cgcttcctgg
     1081 aaccggccat ggcttatctt aaaaaagtct gttccatgtt ggaacctctg cagattacca
     1141 agggaggccc catcatcatg gcccaggtgg aaaatgaata tggttcctat ggttctgaca
     1201 aggattacgt gaaaaagcat ctggacgtta tccggaagga acttccggga gttgttccct
     1261 tcacgtcgga cggcccgaac gactggatga ttaagaacgg cacgcttccg ggcgttgttc
     1321 ccgccatgaa tttcggcggc ggagccaagg gcgcctttgc gaatctggag aagcacaagg
     1381 gtaaaacgcc ccgcatcaac ggcgaattct gggtgggctg gtttgaccac tggggcaagc
     1441 ccaagaatgg cggcagtacg gaaggtttca accgagacct gaagtggatg ctggaaaata
     1501 aagtctcccc caacctcttc atggtgcatg gggggacctc cttcggcttc atgaacgggg
     1561 cgaactggga aggcgcctat acgccggatg taaccaatta cgactacggc gcccccattt
     1621 ccgaaaacgg aaccctgacg gaccgctacc gcaccttccg ccagactatt caggattatt
     1681 acggtgatac gtacaagctt cccgaacctc ccgcccagcc ggaaatgatg gagctgcccc
     1741 ccatcacgtt tacggaaacg gccggcatgt tttcccgtct tcctcagccc tccatccgca
     1801 aggagcccgt ccacatggaa gccctggggc aaagcctggg tttcatcctg taccggacaa
     1861 aggtgcaagg accggtgaaa ggagaactga agatgaacaa catgcaggac cgcgccatcg
     1921 tttacgtgga tggcaagagg cagggggcgg cggatcgccg ttacaagcag gattcctgtg
     1981 atgtggtgat tccttccgga cttcatacgg tggacatttt tgtggaaaac atgggccgca
     2041 tcaacttcgg cggccagata cagggcgagc gcaagggcat ccgagggccc atcacgctgg
     2101 acggcaaaaa gctggaaaac ttccttatct acaacttccc gtgcaaggga gtggagcttc
     2161 ttcccttctc cggcaagaag ccggcgggcg accagcccgt gttccaccgc gggtatttca
     2221 acgtttccaa tcccaaggat acctacctgg atatgcggga cggctggaag aaaggcgttg
     2281 tgtgggtgaa tggacgcaat ctgggccgct tctggtttat cggctcccag caggctcttt
     2341 attgccccgg agaatacctg aagcctggga aaaatgaaat cgtggtgctg gacgtggacg
     2401 gaggtttcgg cacggtgaag ggtgtgaagg aagccattta tgaagtcaac agggaccccg
     2461 ccatggcgga tgtcttccgc gtaggcaaac ctgtggcccc cgctgccggc cagctggtgc
     2521 acaagggttc cttcgccaag ggggcagatc agcaggaaat caaattccgc gctcccgtcc
     2581 aggcccgtta catcgccatt gtcagtaaaa acgcccatga caacggcccc catgccgcta
     2641 ttgcggagct gaacttcctg gatgcctccg gcaacctgct tccccgcgaa cagtggtccg
     2701 tggtttatgc ggattcccat gaaacgacgg gggaagccgc ccaggcgggc ctggtgatgg
     2761 acaaccagcc caccacctac tggcatacca agtggcaggg ggacaaccct cagcatccgc
     2821 acatgatcgt gctggatctg ggcaaggtgc agaaactttc aggattccgc tacctgccgc
     2881 gccagaacag ggaaaacggc cgcatcaagg actatgaagt ctatgcgtct cccaagccgt
     2941 tcaagcctgc caagtaaggt tctctgaatt gttgaacttt tcagggccgc ttcctccagg
     3001 atgagggggc ggccttgttg cgtccggaca tcccggaaga aacagttctc ctttgcccgg
     3061 ataagagccg ttccggttta aagatgcgta tcagctttcc gtccctttgt ttcctgctgt
     3121 tgtgcgggcc ggtattccat gcgcaaaaaa ggcggttccg cagtataacg gagccgccgg
     3181 aagaccgtaa taaacctgat aattgtttgt tagaaattcc aggagaagcc cagcttgatg
     3241 gtgtggtagc cggggtcatt gtagccctga atctggtaat attcgtagcc gagcacgaag
     3301 tccgcgctat tggagatgct gaacttgaag ccggcctggg cagcccagga aaacttggtg
     3361 gaggaaatgg ttcgggaacg gctgccgtct tccagtccat gaatggtggt cttcacgtcg
     3421 ccgtaagtgg cgccgacttt gccgcccaga tagaacatgg tgtagtcgcc gatgggaagg
     3481 ttcagcgtgt aaccggccat catcgggata taggtggaac ggaggctggc ggtataaggc
     3541 ccgctgatgc ccaggtcctg cacgctgggg tggtgggaac cggccaggat gccgccgttc
     3601 agggaaatct gatgaacaat gctgccggtt tcaatatagt tgaaaatgct cagattgccg
     3661 ccggccacgt tgggcatggc gtctttggtg gcgattccgt aagccccttc cagggacatg
     3721 ccgaactggc cgtcgtactt gtcggcatga taggcgctgg cggaagacac ggaggccgca
     3781 agggccaaac ctaacagtat agttttgttc atatggggat aaatggtgaa tttaactgtg
     3841 gtccattctt ggcagttttc tcttttgaag tcaaacatga aatgtcaaat ggacgcgaaa
     3901 tcctcaaacc cggggagatg aagttgccag aacgctcccg gtggggtaca ttctgcgcca
     3961 tggaacaaca agcgtgtgat ttgttggtga ccgctttccg catgctgggc ggagcggaac
     4021 agtccagcgc tgccggagat gcggccgtcg ccgttcggga cgggaaaatt ctggaaacgg
     4081 gaagcgccgc agaactggaa gccaggtgga cgccctccgt tcgcagggat ttggggaatg
     4141 cgctgctgat gccggggctg attaatgccc atacgcatgt ccccatgacc tttttgcgcg
     4201 gttttgcgga tgacttgccg ctgatggaat ggctgaccgg gcatattttc ccggtggaag
     4261 cccggctgac ggataaaatc gtctccctgg gggcccggct gggcatgtat gaaatgatgc
     4321 ggacgggcac cacggcgttt gtagattcct acctgttgga ggccaatgtc ctccaggaag
     4381 cggaacgcat gggaatgcgc tgtgttggcg gggaagtcgt ttttgctttt ccctctccgg
     4441 catacggggg gtgggatggt gcggaagccc tgtaccggga gcaggcggaa cgcttttccg
     4501 gccgcggcag ggtgcaggtg gccctgatgc cgcacagcgt gtacactacc agtgacgaag
     4561 tgctccggcg ttccatgaag ctggcggagg aactggatat gatgctgcat atccatctgt
     4621 ccgaatccgc cggggaagtg gagcagtgcc gttccctgca cggaggaagg cgccccgtgg
     4681 gctatgcccg cgatatgggg ctgctgaatg aacgcgccgt gctggctcat atggtggacg
     4741 tgacggatga ggaactggag ctggtggccg cttccggggc ggcggttgtc cataacccgg
     4801 tcagcaacct gaaactggcg tccggcttcg cccgcgtgcg ggatatggtg caggccggcg
     4861 ttcccgtctc tctgggaacg gacggagcgt gcagcaacaa cagtctggat atgtttgaaa
     4921 caatgaagct ggccgctatt cttgccaagg ggtacagcgg tgacgctact gcgctccccg
     4981 ccatgcaggc gctgaagatg gctacggcgg agggcgcccg cattttccgg acgccggggc
     5041 tgggaacact ggtcccgggg gctcccgcgg atatgatcgc gctgaacctg gatgaaccga
     5101 acctgtgccc gatgttcaac gaaacgtccc atgccgttta tgcctcttcc ggcaaggatt
     5161 gcgtgttcac catggtggaa gggagaattt tgtatgatca tggaatttac acggacggcc
     5221 tgtatgcgga taccgccgcg gaaatgcggg atctggtaaa gtgggtgaaa aacagtgatt
     5281 aagtattgga aatgagcgaa taaaggcagg tttccaacac atgctttaat cacccttccc
     5341 ttacctctgc tatctgtcgg cctcccga
//
LOCUS       DAOMSU010000146         5353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1361_length_5353_cov_2.635523, whole genome shotgun sequence.
ACCESSION   DAOMSU010000146 DAOMSU010000000
VERSION     DAOMSU010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5353
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1361_length_5353_cov_2.635523"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1805
                     /locus_tag="PK077_06980"
     CDS             <1..1805
                     /locus_tag="PK077_06980"
                     /inference="COORDINATES: protein motif:HMM:NF015737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HRN24083.1"
                     /translation="ALKLSGAEMTINSSASHAFTGTLDGASGTLNVKSGNGSVQTIKG
                     AGNAGYHLNVSDGGVLTLAGAAGEGGNPARASYQGIAVNGGTLNIGSADIPKTQLTLG
                     SDGLSVTGDSTVTITTSTTTVDGLGNPFVTSSGNITLGDGTGEVTLVMNNLDTLVSGS
                     AETLNMELFKTTDGALVSLGDNVTLQDMILGSMYENLELTTNDSMTAIILTGTARTEN
                     IFRNSSLTRNAASGADILWNSRYHMEEGTTLRDFYTSVLLSQNSGDYSGAARKLAAAA
                     GSTVTSLGIAQRDSLRDQMGWIRNRVAQMGVNPAYVHEDMPYFHMWMQGTGSYAKLDT
                     KGDESGYELTTWGGTVGMDVDINDRFTAGAAFTANYGNLTASAADTADGDLDSYYVNL
                     FARYQYRKWSHLLILTGGWNDAALTRTVDYGTGAYQARGNTTGAGFGAMYELAYDIAL
                     NEDRSVILQPLFNASIVTTRMDGYRESGSAGNAGLRVEDQELTTAAVAVGARLSGLMG
                     SNVFGREALGEVRVNVSQDMGDRRGQANVGFLADPSYTRPVYGAKVGSTAFQIGAGLS
                     VPVGTQGVIFVDGNADIRSGSSSINGSIGYRYNF"
     gene            complement(1927..2337)
                     /locus_tag="PK077_06985"
     CDS             complement(1927..2337)
                     /locus_tag="PK077_06985"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRN24084.1"
                     /translation="MTDHYYTSETENVGQFRCETTFIPSNVTFATDLPASFEGRAEFP
                     SPGVLLGSTLASCMMSLVSVVAARKGVDLKGMRVLARAAESSKGIEKIELKAIMPLEG
                     SHPLRAMLEKTALSCPVRQALRPDLETPIEWEWK"
     gene            complement(2536..3699)
                     /locus_tag="PK077_06990"
     CDS             complement(2536..3699)
                     /locus_tag="PK077_06990"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HRN24085.1"
                     /translation="MQLPAVQTKFLNLPNSFSLRNGATLDKVCVAYEQYGTLTPEKDN
                     AILLFHALSGSQHAYGYNPEVPGTGSLWKPENHEGWWNSIIGPGKPLDTDRFCIICAN
                     YLGGCYGTTGPATPCPADGQPYGSRFPHVEIADQARLQALLLDSLGIERVHLMGPSVG
                     GLIALSFACQFPERVRSFISIGSGYRASIEHRLSLFEQILAIELDPDFQGGDYYRGPA
                     PKKGLAFARIIGHKSFVYQEGLEQRARKDVGGNYGLLTWMTPTRSTQSYMLHQGTKFA
                     ERFDANSYIRIADMWAEFDIRDHTPDGTFQTALEGFRRAGIPALIFSIDTDCCFRPAE
                     QQDFAAQLEAAHIPTEFHTIASTKGHDSFLLEPELYAEPIRRILAARKPKGTA"
     gene            complement(3787..3951)
                     /gene="ykgO"
                     /locus_tag="PK077_06995"
     CDS             complement(3787..3951)
                     /gene="ykgO"
                     /locus_tag="PK077_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HRN24086.1"
                     /translation="MKVLSSLASMKRRHADCQIVKRKGTLYVICKSNPKFKARQGATA
                     GTRLSKKGVK"
     gene            4239..>5353
                     /locus_tag="PK077_07000"
     CDS             4239..>5353
                     /locus_tag="PK077_07000"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRN24087.1"
                     /translation="MTDSSSPDSSPASRNTFAHHLGMKGFLLMEGLLKLVGMKTLYRL
                     GRAAGAVAWYLLPQRRNIVERNLRIVLNPALRGKELQKLSRENFKRTIANFLCSAKTA
                     TLTDEQLKHCVTVGGHEQFAAPVLEGRGQVCAIAHSGNWEALARIRAFYPEVERYGSM
                     YRQFDNPLMEEYVYQRRTERGTQMFSKEGGIKAPMKMLKEGGALGVLSDQFVWEGVYV
                     PFFGKVTGTTPLPALLRKRAGADMVAIAVRTDAPGHWIADMGNVVDFSGSDGSLAGDT
                     IEVNRGLETLIRESVLDVFWMHHRWKSVDRFAPQDKKTAALLENMELKPYRILVAVPG
                     ALDEALATVPLIRALKTVRCDMQVNVICPSAQMGIWKT"
BASE COUNT         1124 a         1395 c         1676 g         1158 t
ORIGIN      
        1 gagctctgaa gctgagcggt gcggaaatga cgatcaattc ttccgcctcc catgccttta
       61 ccggtacgct ggacggggca tccggaacgc tcaacgtcaa atccggcaac ggaagcgtcc
      121 agactatcaa gggtgcaggc aatgcaggtt atcacctgaa tgtgagtgac ggcggcgtgc
      181 tgaccctggc cggagcggca ggggagggcg gtaatcctgc ccgggcctcc tatcagggaa
      241 ttgctgtgaa cggcggcacg ctcaatattg gcagtgcgga tatccccaaa acacagctga
      301 ctcttggctc ggacggtctg agcgtaacgg gggacagcac ggttaccatt actacctcca
      361 ccaccacggt ggacggactg ggcaatcctt tcgttacttc cagcggcaac attacgctgg
      421 gagacgggac gggggaagtc acgctggtca tgaacaatct ggataccctg gtttccggga
      481 gtgcggaaac gctgaatatg gaattgttca agacgacgga cggcgcgctg gtgtccctgg
      541 gtgacaacgt caccctccag gacatgattc ttggttccat gtatgaaaat ctggaactga
      601 ccaccaatga cagcatgacg gccatcatcc tcaccggcac ggcgcgcacg gaaaacattt
      661 tccgtaactc gtccctgacg cggaatgctg cctctggagc ggatatcctt tggaattccc
      721 gctatcacat ggaggaaggc actactttgc gcgacttcta cacctctgtt cttctttccc
      781 agaactcagg cgactattcc ggcgccgccc gcaaactggc cgccgccgcc ggcagtacgg
      841 tcaccagcct gggcatcgcc cagcgcgact ccctgcgtga ccagatgggc tggatacgca
      901 accgcgtggc gcagatgggc gttaaccctg catatgtcca tgaagacatg ccatacttcc
      961 atatgtggat gcagggaacc ggatcttatg ccaagctgga caccaagggt gatgaaagcg
     1021 gctacgaact tactacctgg ggcggcacgg taggaatgga tgtggatatc aacgaccgct
     1081 tcactgcggg agctgcgttt acagccaatt acggaaacct gactgccagc gcggcggata
     1141 cggcagacgg agacctggac agttactatg tcaatctgtt cgcccgctac cagtaccgta
     1201 aatggtccca tctgctgatt ctgacgggcg gctggaatga tgccgctctt acgcgcacgg
     1261 tggactatgg tacgggggct taccaggccc gcggcaatac gacgggagcc ggtttcggcg
     1321 ccatgtacga attggcgtat gacatcgctc tcaatgaaga caggagcgtg attctccagc
     1381 ctttattcaa cgcttccatc gtgacgaccc gcatggacgg ctatcgggaa agtggttctg
     1441 ccggaaatgc cggattgagg gtggaagacc aggaactgac cacggcggct gtcgctgtgg
     1501 gagcccgtct ctccggcctc atggggtcca acgtcttcgg gcgtgaagcg ctgggagaag
     1561 tacgggtgaa cgtttcccag gacatggggg accgccgcgg acaggccaat gtgggcttcc
     1621 tggcggatcc ctcctatacg cgtccggtgt atggcgccaa ggttggaagc acagccttcc
     1681 agattggtgc ggggctgagc gtccccgtgg gcacccaggg cgtcattttt gtggacggca
     1741 acgcggatat ccgcagcggt tccagctcca tcaacgggag tatcggctac cgctacaact
     1801 tctaaaggaa aaaacgtcag ccaattggaa agccgctgcg gaatcatccg cagcggcttt
     1861 tttcatgaat ggacgggatt tctttaagga taacttcaag cttgggttga cttccctgat
     1921 ggctgtttac ttccattccc attcaatggg cgtttccaga tcgggccgca gcgcctggcg
     1981 cacagggcag gacaaggcgg ttttttccag catggcgcgg agagggtggc tgccttccag
     2041 gggcatgatt gccttcagtt caattttttc aattcccttg gatgattccg ccgcacgcgc
     2101 cagaacgcgc atgcctttca aatccactcc ttttctggcc gcgaccacgg aaaccaggct
     2161 catcatgcag gaggcgagcg tggaaccgag caaaactccg ggggagggga attctgcgcg
     2221 gccttcaaaa gaagcgggca ggtccgtggc aaacgtaacg ttggaaggga taaaggtggt
     2281 ttcgcagcgg aattgtccga cgttttccgt ttcggaggtg taataatgat cagtcatgaa
     2341 gatgtgacag cgaaaaccct gttcgggttc atccggctca ggttattgtc acgttgaaga
     2401 gccgtgaagt tccgctttgc aaggaacctg gccgttcctt catgacatgg ttgatttttc
     2461 cctctcgctt ccggttcccg gcatcatttc tttttaagcg ctgctccgcc gtgtcttctt
     2521 gcggcggaaa aaagcttacg ccgtcccctt cggcttcctt gcggccagaa tgcgccggat
     2581 gggttccgca taaagctccg gctccagcag gaaggaatcg tgtcccttgg tggaagcgat
     2641 ggtatggaac tccgtgggaa tatgggcggc ttcaagctgc gccgcgaaat cctgctgttc
     2701 cgccgggcgg aaacagcagt ctgtatcaat ggaaaagata agcgcgggaa tccctgcgcg
     2761 gcggaagcct tccagggcgg tttgaaatgt tccgtccggg gtgtggtcgc ggatgtcgaa
     2821 ctccgcccac atatccgcaa tgcggatata ggagttggcg tcaaaacgtt ccgcgaactt
     2881 ggtcccctgg tgaagcatgt agctttgcgt gctgcgggtg ggggtcatcc acgtgagcag
     2941 gccgtagttg cctcccacgt cttttctggc gcgctgttcc agcccctcct ggtaaacaaa
     3001 tgatttgtgg ccgatgatac gggcgaatgc cagccctttt ttgggcgccg gcccccggta
     3061 ataatccccg ccctggaaat ccgggtcaag ctcaatggcc aggatttgtt caaacaggga
     3121 caggcggtgt tcaatggaag cccggtaacc ggagccgatg gagatgaagc tccggacgcg
     3181 ttccgggaat tggcacgcga aactgagggc gatcaggccg cccacagagg ggcccataag
     3241 atgaacgcgt tctatgccca ggctgtccag cagaagggcc tggaggcgtg cctggtctgc
     3301 gatctccacg tgagggaagc gggagccgta gggctggccg tccgcagggc agggggtagc
     3361 ggggcctgtg gtgccgtagc accctcccag gtaattggcg cagatgatac agaagcggtc
     3421 cgtgtccagc ggcttgccgg ggccgatgat actgttccac cagccttcgt ggttttccgg
     3481 tttccagagg gagccggtgc ccgggacttc cggattgtag ccataggcgt gctggctgcc
     3541 ggaaagggca tggaacagca ggatggcgtt gtctttttcc ggcgtcaggg ttccgtactg
     3601 ttcataggcg acgcatacct tgtcaagcgt agctccattc ctcagggaga aggagttggg
     3661 aagattcagg aatttggttt ggacggcagg aagctgcatg gatgtacgga aaggggcttg
     3721 gtcagcaaaa aaaagaccgg cacccggcgg gcggcggtct tcttgaaatc tgtgctgggc
     3781 cagggattac ttgacgccct ttttggaaag gcgcgtgcct gccgtagcgc cctggcgggc
     3841 cttgaacttg ggattgctct tgcagatcac atacagcgtg cccttgcgct tcacgatttg
     3901 gcagtctgcg tggcggcgct tcatagaggc taaggaagaa agaactttca tggtgtataa
     3961 ctccggtttg gtgttcggtt cgggagacgg aatataccgt ttagttaaga attgtaaagt
     4021 catttctttg ccaaaagtaa gatgccgtct ccgcagagat gcttttatga ggaagatgca
     4081 gtaaagcatg ggatggcttt ggattccgcg ccggcggaaa aatttaggga agacgtcatt
     4141 tatccgagaa aataaaatgc gagaagggag ctcagccggg ggaataaagg gtttgtggac
     4201 cggaggggat tcgtataaca tcttttccgg ttttccttat gactgattct tcctctcctg
     4261 attcctcccc ggcttcccgg aatacgtttg cccatcattt gggaatgaaa gggtttctgc
     4321 tcatggaagg gctgttgaag ctcgtgggca tgaaaacgct gtaccggctg gggcgggcgg
     4381 caggggccgt ggcgtggtat ctgcttcccc agcgccggaa cattgtggaa aggaatctgc
     4441 gcatcgtttt gaatccggcc ctgcgcggaa aggaactgca gaaattaagc cgggaaaatt
     4501 tcaaaaggac cattgccaat ttcctgtgtt ccgcgaagac ggctaccctg acggatgagc
     4561 agttgaagca ttgcgtgacg gtcggagggc acgaacagtt tgccgcgcct gtgctggaag
     4621 ggcgcgggca ggtttgcgcc atagcccact ccggcaactg ggaggcgctg gcgagaatac
     4681 gcgcatttta cccggaggtg gagcgttacg ggtccatgta ccgccagttt gacaatccgc
     4741 tgatggagga atacgtgtac cagcggcgca cggaacgtgg aactcagatg ttctccaagg
     4801 agggcggcat caaggctccc atgaaaatgc tcaaggaagg gggggccctg ggcgtgctga
     4861 gcgaccagtt cgtatgggag ggggtgtatg tccccttttt tggcaaggtg accggcacga
     4921 ctccgctgcc cgctctgctg agaaaaaggg ctggcgcgga catggtggcc atagcggtgc
     4981 gcacggatgc tcccggccac tggatagcgg atatggggaa tgtggtggat ttttccgggt
     5041 cggacggctc tctggcgggg gatactattg aagtgaacag ggggctggaa acattgatcc
     5101 gggaatccgt gctggatgtg ttctggatgc accaccgctg gaaatccgtt gaccgatttg
     5161 ccccgcagga taaaaaaacg gctgcgctcc tggaaaatat ggagctgaag ccgtaccgca
     5221 ttcttgtagc cgttcccggg gcgttggatg aagccctggc gaccgttccc ctgattcgtg
     5281 ccctgaaaac cgtccggtgc gacatgcagg tgaacgttat ctgcccgtct gcccagatgg
     5341 gaatatggaa gac
//
LOCUS       DAOMSU010000147         5326 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1377_length_5326_cov_2.783153, whole genome shotgun sequence.
ACCESSION   DAOMSU010000147 DAOMSU010000000
VERSION     DAOMSU010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1377_length_5326_cov_2.783153"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..879)
                     /gene="glgP"
                     /locus_tag="PK077_07005"
     CDS             complement(<1..879)
                     /gene="glgP"
                     /locus_tag="PK077_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRN24088.1"
                     /translation="MAKSFLPTIFEHPYEIDPKYGKSVAYFSMEYAIDNSFKIYSGGL
                     GYLSGSHMRSAHDLRQNLVGVGILWSYGYYNQIRAEDGSMATQYMRKNYPFLEDHNIK
                     FLIHVCGAPVWVKAYFLNPETFGTAPMFFLSTDLEENDEESRNISRRLYDANGFTRIA
                     QYVLLGKGGARLFDELGIEPEIYHLNEAHGLAAAFHVLAKTGSVEEVRKRFVFTTHTP
                     EEAGNEKMDVNTMNTFSFFDGLSMEQVRAAVGMTDNTFNYTLAALRLSHIANGVSKLH
                     GEVSRQMWKDYEGICPI"
     gene            1149..2177
                     /gene="tdh"
                     /locus_tag="PK077_07010"
     CDS             1149..2177
                     /gene="tdh"
                     /locus_tag="PK077_07010"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HRN24089.1"
                     /translation="MKALVKTQAGPGLELMDVPMPEVGPNDVLIKIHKTAICGTDLHI
                     WNWDKWAQQTIPVGMHVGHEFCGVIESVGSSVTEYKPGEIVSGEGHIVCGHCRSCRSG
                     QKHLCPNTKGVGVNRPGCFAEYLSIPQDNVVRIHKSIPMEIASIFDPLGNAVHTALSW
                     DLVGEDVLITGAGVIGCMAAAVCKKAGAKTVVITDINDFRLGLAKTLGADRTVNVTRE
                     KLEDVMKELEMTEGFDVCLEMSGAPSCLKDIIDNSRNGANISLLGIQPDGSSIEWNKF
                     IWKGLKMKGIYGREIFETWHKMDSMIRSGLNVAPIITHRLPYTEFREGFEAMNSGKSG
                     KVVLDWIV"
     gene            complement(2290..2934)
                     /gene="def"
                     /locus_tag="PK077_07015"
     CDS             complement(2290..2934)
                     /gene="def"
                     /locus_tag="PK077_07015"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRN24090.1"
                     /translation="MILDILQYGHPLLREECGPVVHINRDILSFLDDMQETLAQGGIG
                     LAAPQVGRPIQLVTINIPSTDATTTWLEVDGCPTTLSRIMPLNFINPILHPFGKKVPY
                     REGCLSITKVYANVMRRSCVRAVLTMMDGRTVTVKCNGLLARCLQHEVDHLHGGLFTD
                     LVSPGDHDKVIRRLRLTHPDAFEEDDDSYARRMKEKRRAQARKARMPQPPGKTA"
     gene            3147..4403
                     /locus_tag="PK077_07020"
     CDS             3147..4403
                     /locus_tag="PK077_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24091.1"
                     /translation="MKLSSCFSLFPNLPLMGGALLLALCLCSCGDKGKEAGAAEAGKS
                     ERLSPVESYEELLSLRLKEVEQMTDLVVSIQDRTAGEALMNELGRLTEKFKFYDMNCG
                     RLMALNPRVHEESRKMHPAFHSILRERVDKARDRMMSGILKLHEFRYYDSDVIRNDLE
                     KCGLSLTDERVALYLNNKIKPFLKAYLEQYSTMVDMLEGIDNKVAAEAYAVSVALQGR
                     MVREQMNNIARMEKKYGDWIPGFERHFHDGLEKMRKKAEDERKRAFRALLKLTTARMY
                     DSPSMQKAVEGLKIEQPRLIDFQEFWADPVAAMEEFYMCLERIDFLLRSIKDAGTADQ
                     CAMELVEVVAKLKRLRYVVDDFQKSGRMNLVKTRDMERISRRAEEAEMAVSASMARLI
                     VETDVVTRSPLLSRAMGLYRDVMYAR"
     gene            4441..4575
                     /locus_tag="PK077_07025"
     CDS             4441..4575
                     /locus_tag="PK077_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24092.1"
                     /translation="MEYDNQPMKDVDFSGSTVVVTGGACGIGCAIAEAFAVAGGRVVF
                     "
     gene            4578..>5326
                     /locus_tag="PK077_07030"
     CDS             4578..>5326
                     /locus_tag="PK077_07030"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRN24093.1"
                     /translation="MKHEETAFLLEELPGEGHLCMTGNAGVEEDVRSFAEKVLECFGR
                     VDVLINNACITHRGILSGCSFKEFNEVLRVGVSAPYLLNLLFRDHFSRGASIVNIAST
                     RASMSRKDTESYSAAKGALTSLTHALSMSLSPFGVRVNSVSPGWIDTGLFGVLYPEDA
                     FQHPSGRVGSPEDIVKAVFFLCGSSFVNAENLVIDGGMSRMMVYHGDEGGIPPGTVGQ
                     RLLLMGLRFRHAFHCSCFKQVPCRPDAYYAW"
BASE COUNT         1154 a         1253 c         1608 g         1311 t
ORIGIN      
        1 gatggggcat ataccctcat agtccttcca catctggcgg gatacttctc catggagttt
       61 ggaaacgccg ttggcgatgt gggagaggcg cagggcggcc agcgtgtagt tgaacgtatt
      121 gtccgtcatg cctacggcgg cgcgcacctg ttccatggag agcccgtcga agaaggagaa
      181 cgtattcatg gtgtttacgt ccatcttttc gttgccggct tcttccgggg tatgggtagt
      241 gaaaacgaaa cgcttgcgca cttcttccac gctgcccgtc ttggcaagca cgtggaaggc
      301 cgcagccagg ccgtgggctt cgtttagatg gtagatttcc ggttcaatgc ccagttcgtc
      361 aaaaaggcgg gcgcctcctt tgcccagcag tacgtactgg gcgatgcggg tgaagccgtt
      421 ggcgtcgtac aggcggcggg agatattgcg gctttcctcg tcattttcct ccaggtccgt
      481 ggaaaggaag aacatgggcg cagtgccgaa tgtttccgga ttcaggaagt aggcttttac
      541 ccagacggga gctccgcaga catgaatgag gaatttgatg ttgtgatctt ccaggaacgg
      601 gtagttttta cgcatgtatt gcgtggccat ggatccgtcc tccgcacgga tctggttgta
      661 gtagccgtag ctccacagaa tgccgacgcc tacgaggttc tggcgcagat catgcgcact
      721 gcgcatatgg gagccggaca ggtagcccag accgccggaa tagattttaa atgaattatc
      781 aatggcgtat tccatggaga agtacgcaac gcttttccca tatttggggt ctatttcata
      841 cgggtgctcg aaaatggtgg gtaaaaatga tttcgccatg gttctgtaaa tagagtaaag
      901 tgttcaaaag gagaactcgc acgccttggt atcatgattc cttcctgttg gaaagggcaa
      961 attgcgtgtg tgtctgaatt gtcgattgaa caggaatgtc tttccggcgt ttgtcgcccg
     1021 ccgctgctcc gggcgtttcc atgggaaggg aagggggggc ggaagcctcc gttccctagg
     1081 ctttttcggc gctccgaaat gaattttgag cttgccgacg caggggaaaa agagcatgaa
     1141 tggggggcat gaaagctctt gtaaaaacgc aggctggccc cggtttggaa ttgatggatg
     1201 ttcctatgcc ggaagtcggc ccgaatgacg tcctgatcaa aattcataaa acagccattt
     1261 gcggcacgga tcttcatatt tggaattggg ataaatgggc ccagcagacc attccggtag
     1321 ggatgcatgt gggccatgag ttctgcggcg tgattgagtc cgtaggttct tccgtgacgg
     1381 aatacaagcc cggggagatt gtttccggtg aggggcatat tgtctgtggc cattgccgca
     1441 gctgccgttc agggcagaag cacttgtgtc ccaacacaaa gggtgtggga gtcaacaggc
     1501 ccggctgctt tgcggagtac ctttccattc cgcaggataa cgtggtgcgc atccacaaga
     1561 gcattccgat ggaaatcgcc tccatttttg acccgctggg caacgccgtc catacggctt
     1621 tgtcctggga tctggtaggc gaggacgtcc tgattacggg agccggggtt atcggctgca
     1681 tggctgccgc cgtctgcaag aaggccggag ccaagacggt ggttattacg gacatcaatg
     1741 atttccgcct gggtcttgcc aaaacgctgg gggcggaccg aaccgtgaac gtgacccgtg
     1801 aaaagctgga agacgtgatg aaggaactgg aaatgacgga gggatttgac gtgtgcctgg
     1861 aaatgagcgg cgctccgtcc tgcctgaagg acatcatcga caattcccgc aacggagcca
     1921 acatttccct gctggggatt cagcccgatg gttccagcat cgagtggaat aagttcattt
     1981 ggaaagggtt gaagatgaaa ggcatttatg gacgtgaaat ttttgaaact tggcataaaa
     2041 tggattccat gatccgcagt ggcttgaatg tggcgcccat catcacgcac cgtctgccct
     2101 acacggaatt ccgggaaggg tttgaagcca tgaattcggg aaaatccggc aaggttgttc
     2161 tggactggat tgtttgattc tctctttttc tttccatgtg attatggatg ccccggtttc
     2221 ctggagggag accggggcgt tttttaggga agggcagtaa aaagggggga taggtttgtt
     2281 gcctgaacgt caggctgttt ttccgggagg ctgcggcatc cgggctttcc gtgcctgggc
     2341 gcggcgtttt tccttcatgc ggcgagcgta gctgtcgtca tcttcttcaa aagcgtccgg
     2401 atgggtcaat ctgagcctcc gtatcacctt gtcatggtca ccgggggaaa ccaaatccgt
     2461 aaacaggccg ccgtgaaggt ggtctacctc gtgctgcagg cagcgcgcca gcagtccgtt
     2521 gcatttcacg gtcacagtcc gtccgtccat catggttaaa acagccctta cgcaggagcg
     2581 gcgcatcacg ttcgcatata ctttggtgat gctcaggcac ccttcccggt acggcacttt
     2641 tttgccgaag ggatgcagga tgggattgat aaaattcaag ggcatgattc gggacaacgt
     2701 agttgggcat ccgtccactt ccagccaggt agtggttgcg tctgtggaag ggatgttgat
     2761 ggtgaccagc tggatgggcc tgcccacctg cggcgcagcc aggcctatgc ctccctgggc
     2821 cagggtttcc tgcatatcgt ccaggaagga aaggatatcc ctgttgatat gaaccacggg
     2881 cccgcattcc tctctcaaaa gaggatgtcc gtattgaaga atatcaagaa tcacggcaga
     2941 caggatgata tggataaaag agtgtgacat cccgattcag aagagtgttc attatattaa
     3001 atgaatattt ttcccgtcaa aggaacggaa atgtttttcc cggatgaaat gaaaaggcct
     3061 tgaactttga tgacgctttt ttacggtagt tccggtgccg cgatgcgggc cggagtttca
     3121 ttctccctcc tcaacgctac gttctcatga agcttagctc ctgtttttct ctgttcccga
     3181 atttgccgtt gatgggaggc gcgctcctgc tggcgctgtg tttgtgttcc tgcggggata
     3241 agggaaagga ggccggggct gcggaagcag ggaagtcaga aaggctttcc ccggtggaat
     3301 cttatgaaga gctgctttcc ctgcgcctga aggaagtgga acagatgacg gatctggtcg
     3361 tttccatcca ggaccgtacc gcaggagagg ccctgatgaa tgaacttggg cgcctgacgg
     3421 agaagtttaa attttacgat atgaattgcg ggcgcctgat ggctctgaat ccgcgcgtgc
     3481 atgaagaatc gcgtaagatg catcccgcct ttcattccat tctccgggag cgcgtggaca
     3541 aggcccgcga ccggatgatg tcgggaatac tgaagctcca tgaattcagg tattatgatt
     3601 ccgatgtcat caggaatgat ctggaaaagt gcggtctcag cctgacggac gaacgggtgg
     3661 ccctgtattt gaacaacaag ataaagccgt ttctgaaggc ttatctggaa cagtattcca
     3721 ccatggtgga catgctggag gggattgaca ataaagttgc ggcggaagcc tatgccgtat
     3781 ctgtagccct tcagggacga atggtcaggg agcagatgaa caacattgcc agaatggaga
     3841 agaagtatgg agactggatt cccggttttg aacggcattt ccatgatggg ctggaaaaga
     3901 tgagaaagaa ggcggaggat gagcgcaaac gcgctttccg ggcgctcctg aaactcacga
     3961 cggccaggat gtatgattcc ccttccatgc aaaaggcagt ggaggggctc aaaatagagc
     4021 aacccaggct aatcgatttt caggagttct gggccgatcc tgtcgcggcc atggaagagt
     4081 tctacatgtg cctggagaga attgactttc tcctgcgcag catcaaggat gccggaacgg
     4141 ctgaccagtg cgccatggag ctggtggaag tggtggccaa gttgaaaagg ttgcgctatg
     4201 ttgttgatga tttccaaaaa tccggacgta tgaatctggt gaaaaccagg gatatggaac
     4261 gtatcagccg ccgtgcggag gaagcggaaa tggccgtttc cgcttccatg gcccgtctta
     4321 ttgtggaaac ggacgtggta acccgttctc ctctcctttc cagagccatg gggctttacc
     4381 gcgatgtaat gtatgcacgt taagtaccgt atgccggaag gcgatgattg actttcttga
     4441 gtggaatatg acaatcagcc tatgaaggat gttgattttt ccggcagtac cgtggtggtg
     4501 acgggaggag cctgcggcat tggctgcgcc attgcggaag ctttcgctgt ggcgggaggc
     4561 cgggtggtgt tttagaggtg aagcatgagg aaacagcctt cctgctggag gaattgccgg
     4621 gagaggggca tctttgcatg acgggcaatg cgggtgtgga agaggacgtg cggtcttttg
     4681 cggaaaaggt gttggaatgt tttggccgtg tggatgtgct gataaataat gcgtgcatca
     4741 cgcatcgcgg cattctttcc ggctgttcct tcaaggaatt caatgaagtt ttgcgcgtgg
     4801 gagtttccgc gccctatctg ctgaaccttt tatttcgcga ccatttttcc cgcggcgctt
     4861 ccattgtgaa tattgcttcc acccgcgctt ccatgtcccg gaaggatacg gaaagctata
     4921 gtgcggccaa gggggccctc acttctttaa ctcacgctct ctccatgagc ctttcccctt
     4981 tcggcgttcg tgtgaacagc gtcagtcctg gctggattga cacggggctg ttcggcgtcc
     5041 tgtatccgga agacgctttt cagcatcctt ccgggcgcgt cggttcgccg gaagatatcg
     5101 tgaaggccgt tttctttctc tgcggctcca gctttgtcaa tgcggagaat ctggtcattg
     5161 acggcggcat gtcccgtatg atggtgtacc acggagatga ggggggcatt ccacccggaa
     5221 cagtcggaca gcgcctcctc ctgatgggct tgcgcttccg gcatgctttc cattgcagct
     5281 gtttcaaaca ggttccgtgc cgtccggacg cttattacgc ctggaa
//
LOCUS       DAOMSU010000148         5320 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1380_length_5320_cov_2.252042, whole genome shotgun sequence.
ACCESSION   DAOMSU010000148 DAOMSU010000000
VERSION     DAOMSU010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5320)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5320
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1380_length_5320_cov_2.252042"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PK077_07035"
     CDS             <1..257
                     /locus_tag="PK077_07035"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRN24094.1"
                     /translation="KRAGNAQGKPVVSDLRDSGSIEQDADQIIMIHRPYMYKPDKHDP
                     TEAQWIIGKNRFGRLGRIQFRWTAELTKYEEEQNYPVTNK"
     gene            254..553
                     /locus_tag="PK077_07040"
     CDS             254..553
                     /locus_tag="PK077_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24095.1"
                     /translation="MRPPKPSLRKNKPTRRGKPGSYKLRLTLLVDPRKKGKLVELGLG
                     TNDRQEAEERANSIINALESAGLYRLPAVRILEHHVAQFGKIEPPPFEHPELPLW"
     gene            598..2142
                     /locus_tag="PK077_07045"
     CDS             598..2142
                     /locus_tag="PK077_07045"
                     /inference="COORDINATES: protein motif:HMM:NF015461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HRN24096.1"
                     /translation="MDSKEWAALNAAMKENKFFSSKVENIRLLERLHRLIKNYLTGEK
                     ETLPNGETVIKVGSAADFSNQALQWLQTEGLVPPDAEGPKYHNDIKNIGALARLKLIF
                     KTNVRQSIGAAQWEASMKPANLKAWPAFRFIRFPGAKTKRLVHVVNEDAVRLKTDFTF
                     WADEMNAASLGGFEVPWPPFGFNSYMDQEPVSREECERLGLLKPGEPLKRPRGAERFG
                     IDLIERYGYGKKASTAKLPEELRAKLKKVYEDRWGVKQDKSDEVVFPSQEVAKKARET
                     AEKVIKVPSAPIPAPVSAVTHTVSLGDVPKVKMPAPLTDKEADDLLRSVTGEVWAKAS
                     RLEKNALFSYTGNGYARINNDLRKGKSNAKAKQIAKVIDRCKVPQDMVVFRGCGVYKE
                     LKDALNWKGEEITDELVDMLNLSVVGNPLKDEGFMSAAVAEGKGFMNRPVLFRILLKK
                     KTRAIYAEPFSRFGAGAGKDWDGLSPQTYFSSEDEIIIQKGGTLKFLQFHNQNGKLII
                     DCELIQ"
     gene            2228..2476
                     /locus_tag="PK077_07050"
     CDS             2228..2476
                     /locus_tag="PK077_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24097.1"
                     /translation="MKCLLCSKKKLNPGSMECSAYERKPDSILYDNADCPSFERCIDA
                     EGLRWIEGYVKLSGKAYVPRQDDIPPAGWEKINKEYAK"
     gene            2473..2961
                     /locus_tag="PK077_07055"
     CDS             2473..2961
                     /locus_tag="PK077_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24098.1"
                     /translation="MKKERTGKKGNVSRYSAALSERICGHIRCGDSLRKAAEKEGIPH
                     PTVMNWARENADFANQYARACEERLAALEDKLLDLVEKGHEVAPRAEIGGTMLQAVKL
                     EIDTLKWMLAKLMPKKYGDRAALALEGGEKNVEVTHKLPAEAIVPLVAALREIWSEEE
                     ES"
     gene            2937..4454
                     /locus_tag="PK077_07060"
     CDS             2937..4454
                     /locus_tag="PK077_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24099.1"
                     /translation="MVRRGRKLGPPVRPEDSPVIFAAVVLGETGLYKWQMKALERAAR
                     GKRVALRAANGSGKTDKVIGILALWFLWRFPRGRMPITSGSWRQVKNQLWPALERHRN
                     NPSLAGWKWLKNCRVETPEGGFIEGFSTNHAGKAEGWHGRVTDEFKDERKEQEEEDPR
                     SEKKARLFDVDEFTGDDPSSPVFFVVDEAKTVPDEIFDAIERCTLQFCIYLSSPGKPE
                     GQFYRCFHEEKDLFCPMVVTAFDCPHISQERIDRILARVGGNEDDSYFRSVVLAEFTL
                     EGDLYIIDPGKLEWGQRQPYEPRRGRPVAFLDIAAGGDETVLAICDGNEAWIEYAERQ
                     RDTVQSVRKCIATLKGLGIADCDLWVDAPGMGLAVISDFNESGWYPNEFFGNNPPEDR
                     DRYINLSAECWNDAGLELMTGRVHIRSRRPDKTLFVQLTTRKKEYADDSRLRNEKKEK
                     MKARNLSSPDRADALLGAIWASFRGSSGVWTGEGNRPIVGKSQHAVKHTGKFYPI"
     gene            4518..>5320
                     /locus_tag="PK077_07065"
     CDS             4518..>5320
                     /locus_tag="PK077_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24100.1"
                     /translation="MRQAANYNVHATESLPQSLALHFISPSGEDMDISGMTLRGAVVQ
                     DGVIMLDCAVTGASTALVTWPRLAAGCGAYDIFLTDASGKEYPLLKGSVHVVSRVTPP
                     DGTNEAAAVAGALDVSIPETEDGSVTIVENPSIVVEELVRQAEAARDEATRLVETLEE
                     QVESGELVNEAVANKLPAALKDAGVELEAVTGQSTLSSGDAADTWTIVGGYAMTWGDE
                     ILAGHLPDSCRLTSISTVYFFTDPALNQYCLRIWKLVNGAYSLIGTSAYV"
BASE COUNT         1346 a         1358 c         1548 g         1068 t
ORIGIN      
        1 aaaaaagagc cgggaacgcc cagggcaaac cggtcgtttc cgacctgcgc gactccggct
       61 ccattgagca ggacgccgac cagatcatca tgatccaccg cccctacatg tacaagcccg
      121 acaagcacga ccccacggaa gcgcagtgga tcatcggcaa aaaccgcttc ggacggctgg
      181 ggcgtattca attccgctgg accgcggaac tcacaaaata cgaggaagaa cagaattatc
      241 ccgtcaccaa caaatgagac cccccaaacc atccctgcga aaaaacaagc cgacgcggcg
      301 aggaaagccc ggatcctaca aactgcgctt aacgcttctg gtggatccca gaaagaaagg
      361 caaacttgtc gagctgggac ttggtactaa cgacagacag gaagccgaag aacgcgccaa
      421 cagcattatc aatgctctgg aatccgccgg actctaccgt cttcccgccg tccgcattct
      481 ggaacatcac gtagcccaat ttggcaagat tgaacctccc ccctttgaac atccagaatt
      541 gcctctatgg taacacccct ggaaaaattc ctggcaaaac atcccacacc ctccggcatg
      601 gattcaaagg aatgggctgc tctgaacgct gccatgaagg aaaacaagtt tttctcttcc
      661 aaggtggaga atatcagatt gctggaacgg ctgcacaggt tgattaagaa ttatctgaca
      721 ggagaaaagg agactttacc caatggggaa acggttatca aggtaggaag cgccgcggac
      781 ttttccaacc aggcacttca atggctccaa accgaggggc ttgttccacc ggacgccgaa
      841 ggcccgaagt atcacaacga tattaaaaac atcggtgctc tggcccgtct gaagctcatt
      901 ttcaagacca acgtccggca aagcattggg gctgctcaat gggaggcatc catgaaaccg
      961 gccaatctca aagcatggcc tgctttccgg ttcatccgct ttccgggagc caagacaaag
     1021 cggcttgttc atgtcgtcaa cgaagatgct gtccggctta aaaccgactt tactttttgg
     1081 gcagacgaaa tgaacgccgc cagcctcggg ggctttgagg tcccctggcc gccgttcggc
     1141 ttcaactcct acatggatca ggagcctgtt tcccgggaag aatgcgaacg gctgggacta
     1201 ctcaaacccg gggagccgtt gaagcgtcca aggggtgcgg agcgcttcgg gattgacctg
     1261 attgaacggt acgggtacgg caagaaggcc agtacggcga agttgccgga ggaactgagg
     1321 gccaaattga aaaaggtcta tgaagaccgc tggggagtca aacaggacaa atctgatgag
     1381 gttgtctttc cctcacagga agtggcgaaa aaggccaggg aaacggcgga gaaagtcatc
     1441 aaggttccct ctgctcccat tcctgcgcca gtctcagccg tcacgcacac ggtcagcctg
     1501 ggagatgtcc ccaaggtgaa gatgcctgcc ccgttgacgg ataaggaagc tgatgacctt
     1561 ttgcgaagcg ttaccgggga agtgtgggca aaggcatcca gactggaaaa gaacgctttg
     1621 ttttcctaca ccggaaatgg atatgcccgc atcaacaacg atttgaggaa ggggaagtcc
     1681 aacgccaagg cgaaacagat cgccaaagtc attgacagat gcaaagtgcc tcaagacatg
     1741 gttgttttcc gtggctgtgg ggtttacaag gaattgaaag acgctttgaa ctggaaagga
     1801 gaagaaataa cagacgagct ggttgatatg ctcaatctct ccgtagtggg aaaccctctc
     1861 aaagacgaag gtttcatgtc tgctgccgta gcggagggga aaggattcat gaaccgtccc
     1921 gtgttgttca gaattctcct gaagaagaaa acccgtgcca tttatgcaga gcccttttcc
     1981 agattcgggg caggggccgg taaggactgg gacggcctta gcccgcaaac ctattttagc
     2041 agtgaagatg aaatcatcat ccagaaggga ggaaccctca aatttctcca attccataat
     2101 cagaacggga aattgatcat tgactgtgaa ttgatacaat aatgatatga aagaagaaac
     2161 atcaccagcg cacaagagaa tttgggagtc tgatttcaaa ggatgcaaaa catcccaccc
     2221 tctcctgatg aaatgccttt tgtgctccaa gaagaagctc aacccgggta gtatggaatg
     2281 tagcgcttat gagcgtaaac ctgatagtat cctctacgat aacgcggact gccccagctt
     2341 tgaacgctgt attgacgcgg aagggctgcg ctggattgaa ggatatgtga aactctccgg
     2401 aaaggcgtac gttccccgcc aggacgatat acctccggca gggtgggaaa aaatcaacaa
     2461 ggagtatgcg aaatgaagaa agagaggacc gggaagaagg gaaatgtttc caggtatagc
     2521 gctgccctct ctgaacgcat ttgcggtcat atacgttgcg gggatagtct gaggaaggct
     2581 gccgaaaagg aaggcattcc ccatcccacg gtgatgaatt gggccagaga gaacgcggat
     2641 tttgcaaacc aatacgcgcg cgcgtgcgag gaacggcttg ccgccctaga agacaagttg
     2701 cttgaccttg tggagaaagg gcatgaagtg gccccacgtg ccgaaatagg gggaaccatg
     2761 ctgcaggcgg tcaagttgga aatagacacg ctcaaatgga tgcttgccaa gctgatgccg
     2821 aagaagtacg gagaccgtgc ggcgctggct ctggaaggtg gagaaaaaaa cgtagaggtg
     2881 acccataaac ttccagcaga agcaatcgtt ccgttagtgg cagccttgag agaaatatgg
     2941 tccgaagagg aagaaagcta gggcctcctg tcaggccgga agattccccc gtcatctttg
     3001 ccgccgtggt gctgggggaa acggggctgt acaaatggca gatgaaggct cttgaacgtg
     3061 ccgcccgcgg caagcgcgtt gccctgcgtg cagccaacgg atccggcaaa acggataaag
     3121 tgatcggcat ccttgctctg tggttcctgt ggcgtttccc ccgtgggcgc atgcctatta
     3181 cgtccggctc atggcgccag gtaaaaaacc agctctggcc tgccctggaa cggcaccgga
     3241 acaacccatc ccttgcgggc tggaaatggc tcaagaattg ccgcgtggaa acgccggaag
     3301 ggggattcat cgaaggcttt tccaccaacc acgccgggaa ggcggaaggc tggcacgggc
     3361 gtgtgacgga cgaattcaag gatgagcgga aggaacagga ggaggaagac ccccgcagcg
     3421 agaagaaagc ccgtctgttt gacgttgacg agtttaccgg ggatgatcct tcttcccccg
     3481 tgtttttcgt ggtggacgag gcaaagacgg ttcctgatga aatctttgac gccattgaac
     3541 gatgtacgct tcaattctgc atctaccttt catccccagg caagccggaa gggcaatttt
     3601 atcgctgttt ccacgaggaa aaagacctct tctgtccgat ggtggtaacg gcctttgatt
     3661 gcccccatat ctcccaggag cgcattgacc gcattctggc ccgtgtgggg ggtaatgagg
     3721 atgattccta tttccgttcc gtcgtgctgg cggaattcac gctggaagga gatttgtaca
     3781 tcattgaccc tggaaaactg gaatggggtc agcggcagcc ctacgagccg cgcagggggc
     3841 gccccgtggc cttcctggac attgccgcgg gcggggatga aacagtcctt gccatctgcg
     3901 acggaaacga agcctggatt gaatacgcgg aacgacagcg ggacacggtg cagagtgtcc
     3961 gcaagtgcat tgccaccctc aaggggctgg gcattgcgga ttgtgatttg tgggtggacg
     4021 ctccgggcat gggcctggct gtcatcagcg attttaatga atcaggttgg tatccgaatg
     4081 agttctttgg gaacaaccct ccggaagacc gcgaccgcta catcaatctc tcggcggaat
     4141 gctggaatga cgccggactg gaactcatga ccgggcgagt gcatatcagg tccaggcggc
     4201 cggacaagac gcttttcgtg cagttgacta cccggaagaa agaatatgcg gacgattcca
     4261 ggctcaggaa cgagaagaag gagaaaatga aggctcgcaa cctgtcttct cctgaccgcg
     4321 cggacgcctt gctgggggct atatgggctt cctttcgtgg atcttccgga gtttggacag
     4381 gagagggcaa caggcccatt gtgggcaaga gtcagcacgc cgtcaaacat acggggaaat
     4441 tttatcccat ttaggactgt tcgtagccca ttttgacatt gttgtaccct ccctcacgtt
     4501 ggggcgataa tgcgtgcatg aggcaagccg ccaactacaa cgtacacgcc acggaatccc
     4561 tgccgcagtc tcttgcgctg cattttattt ctccatccgg tgaggatatg gacatcagcg
     4621 gcatgacgct ccgcggcgcg gtggtacagg atggagtgat catgctggac tgtgccgtta
     4681 cgggggcgag tacggcattg gtgacatggc cgaggctggc cgccggatgc ggcgcttatg
     4741 atatttttct gaccgacgca tcgggaaaag aatacccctt gttgaaggga tccgtgcatg
     4801 tagtgtcccg cgttacgcct ccggatggaa cgaatgaggc cgcggccgtg gccggcgctc
     4861 ttgatgtctc catccccgaa acggaagacg gctccgtaac cattgtggaa aacccgtcca
     4921 ttgtggtcga ggaacttgta cgacaggccg aagcggcccg ggatgaagca acgcggcttg
     4981 tggaaacgct ggaagaacag gtggaaagcg gggaattggt caatgaggct gtagcaaata
     5041 aattgccggc cgcgctcaag gatgcgggcg tggaattgga agcggtgacc gggcaatcca
     5101 ccttgtccag tggagatgcc gccgacacct ggaccatcgt cggaggctac gcgatgacct
     5161 ggggagacga gatactggcg gggcatctgc ctgacagctg ccgcctgacg agtatttcca
     5221 ctgtgtattt tttcaccgac cccgccctga atcagtattg cctgcggatt tggaagctgg
     5281 taaacggtgc gtacagcctg attggcacct ccgcctatgt
//
LOCUS       DAOMSU010000149         5310 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1383_length_5310_cov_2.815224, whole genome shotgun sequence.
ACCESSION   DAOMSU010000149 DAOMSU010000000
VERSION     DAOMSU010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1383_length_5310_cov_2.815224"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2998)
                     /locus_tag="PK077_07070"
     CDS             complement(<1..2998)
                     /locus_tag="PK077_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HRN24101.1"
                     /translation="MMGNSIDHGMFRNSVLLAFGLSISFSGFALAALEEPVPGQQVQA
                     VEGARKINPAAVEVLLDNNRRMTLDFYGDNIFRIFRDDKGGIIRDPKAEPEARILADN
                     PRKPVSRLDVDQKGDAVVITTGKVRIEISKKTSLFKVINLKDHSVVAEQASPILFEKG
                     KTSFSLKAKPDEYFYGGGVQNGRFSHRGKVISIENQNSWTDGGVASPTPFYWSTGGYG
                     VMWHTFKKGQYDFGSREENLVNLSHDENYLDVFFMVNDGPVSLLRDFYQLTGAPVLLP
                     KFAFYQGHLNAYNRDYWKEDEKGILFEDGKRYKESQKDNGGIKESLNGELNNYQFSGR
                     AVVDRYKAHDMPLGWLLPNDGYGAGYGQTDTLDGNIANLKSLADYARKNGVEIGLWTQ
                     SDLHPKPEISALLQRDIVKEVRDAGVRVLKTDVAWVGAGYSFGLNGITDVAQIMTYYG
                     DNSRPFIISLDGWAGTQRYAGIWSGDQTGGVWEYIRFHIPTYIGSGLSGQPNICSDTP
                     MELNMDGWGANEKYPHAFGEPYTSINRWYLKLKSELLPYAYSIAEESVSGLPMIRAMF
                     LEYPNPYTLGKATQYQFLYGPYFLVAPIYQETRADEEGNDVRHGIYLPEGQWIDYFTG
                     DLYEGGKIYNDVDAPLWKLPVFVKNGAIIPMANPSNNVSEINPNLRIYELYPHGSTSF
                     TVYDDDGVTEEYRAGKGVRTLVESRVDDKNNVTVTVHPAVGDFNGFQKKKATEFRINV
                     TQKPSGVSAKIGENSVNLAEANSMEDFKSRENVYFYDRAPNLNRFATKGSDFEKKVIS
                     GNPQLLVKLGAADITAAPTVLSVEGFRFEPAEKHRLSSGALTAPANAAVTEENAAAYT
                     LKPTWDAVPNADFYEIEFGGMLYTTIKGTEFLFEDLEAETPYSFKVRAVNKDGHSAWT
                     AVSAKTKANPLEFAIPGIEGETSVENQGSSLAKLFDFKEKDAWHTKHGEKAVPFDLVM
                     DLKTINKLEKFHYVPREDGGNGTLLKG"
     gene            complement(3503..4399)
                     /locus_tag="PK077_07075"
     CDS             complement(3503..4399)
                     /locus_tag="PK077_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRN24102.1"
                     /translation="MSIILNMDSVGDYNSFLGQETLHPLVSVVDFSRVPPLRHVRKAY
                     GFYAVFLKDLKCGDLRYGRHYYDYQEGTLVFVSPGQVLGNDDNGEYFRIEGYALLFHP
                     DLLRGTSLGRKMRDYTFFSYDASESLHMSERERHLFLNGLTEIRAELERGVDRHTKSI
                     VTANIEVLLNHCMRFYDRQFITREHVNRDVLTEFERLLDSYFEGDKPQEQGLPYVQYF
                     ADELHLSANYFGDLVKKETGKTPQEHIQLKLVDKAKELLCATNKSVSEIAYELGFKYP
                     HHLSRMFKKNTGCSPNEFRLSA"
     gene            4540..>5310
                     /locus_tag="PK077_07080"
     CDS             4540..>5310
                     /locus_tag="PK077_07080"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRN24103.1"
                     /translation="METINGQNMPCRELGKSGLKVSAIGLGCMGMSHAYGAPADKKEM
                     TELLARAVDMGYTFFDTAEVYGTHGCPHDNEELLGKALAPYRDKIVIATKFGIRFSGT
                     AGTGPSPLITDSRPGTIRQSVEGSLKRLGVERIGLYYQHRMDDSVPPEEVAGVMQELI
                     REGKIAHWGISEATEEAIRRAHGTCPITAVQNRYSMMARHHESLFPVLEELGIGFVAF
                     SPLANGLLSGRYDQNSTFEPGTDYRSSMPQFRPESFDAN"
BASE COUNT         1185 a         1533 c         1395 g         1197 t
ORIGIN      
        1 agcctttcag caatgtgccg ttgccgccgt cctcgcgggg aacgtaatgg aacttctcaa
       61 gcttgttgat ggtcttcaaa tccatgacca ggtcaaacgg cacggccttc tctccatgct
      121 tcgtgtgcca ggcatccttt tccttgaagt caaacagctt cgccagggaa ctgccctggt
      181 tttcgacact cgtctcgccc tcaattccgg gaatggcgaa ctcaagcggg ttggctttcg
      241 tcttcgcgct gacagccgtc cacgcggaat ggccgtcctt gttgacggcc cgcaccttga
      301 aggaataagg cgtctcagcc tccaaatcct caaacaggaa ttccgtccct ttgatggtgg
      361 tgtacagcat tccgccgaat tcgatttcat agaaatcggc atttggaaca gcgtcccacg
      421 tgggcttcag ggtataggcc gccgcgtttt cctccgtcac cgcagcattg gcgggagcgg
      481 tcagcgcgcc ggaagaaagg cgatgctttt cagcgggttc aaaccggaac ccttccacgg
      541 aaagcaccgt gggggccgcc gtaatatccg ccgcgcccag tttcaccagc aattgagggt
      601 tcccggaaat cacctttttc tcaaaatcgc tccccttcgt cgcgaacctg ttcaggttgg
      661 gagcccggtc ataaaaataa acattctccc tggacttgaa atcctccatg gaattcgctt
      721 ccgccaaatt aacgctgttt tccccgattt tggcggaaac cccggacggc ttctgcgtca
      781 cgttgatgcg gaactccgta gccttcttct tctggaagcc gttgaaatcc cccaccgccg
      841 gatgaacggt gacggtcacg ttgttcttgt cgtccacccg ggattcaacc agagtgcgga
      901 cgcctttgcc ggccctgtat tcttccgtca cgccgtcgtc gtcatacacg gtgaaggaag
      961 tgctgccgtg cggataaagc tcgtaaatgc gcaggttcgg attaatttcc gagacattgt
     1021 tgctcggatt cgccatggga ataatcgcgc cgtttttgac gaacacgggc agtttccaca
     1081 gaggcgcgtc aacatcatta taaatcttgc cgccctcata caaatccccg gtgaaataat
     1141 caatccactg tccttccggc aggtaaatgc cgtggcggac gtcattcccc tcctcgtccg
     1201 ccctggtttc ctggtaaatg ggagccacca gaaaataggg gccgtacagg aactggtact
     1261 gcgtcgcctt ccccagcgtg tagggattgg gatattccag gaacatggcc cggatcatgg
     1321 gcagtccgga aacggactcc tccgcgatgc tgtaggcgta cgggagcagt tccgacttga
     1381 gcttgagata ccaccggtta atggaagtat agggttcccc gaaggcgtgg ggatactttt
     1441 cattggctcc ccagccgtcc atattcagct ccatgggggt gtccgaacag atgttgggct
     1501 gcccggaaag tccggagccg atgtaggtgg gaatatggaa acggatgtac tcccagacgc
     1561 cgcccgtctg gtcgcccgac cagatgccgg cataccgctg ggttcccgcc cagccgtcca
     1621 gggaaatgat gaacgggcgg ctgttgtccc cgtagtaggt catgatctgg gccacgtccg
     1681 taatgccgtt cagcccgaaa gaatagcccg cgcctaccca ggccacgtcc gtcttcagca
     1741 cgcgcactcc ggcgtcccgc acctccttca cgatatcccg ctgcagcaaa gcgctgattt
     1801 ccggcttggg atgcaggtcg gactgggtcc acaacccgat ttccacgccg ttcttcctgg
     1861 cgtagtccgc cagactcttc aaattcgcaa tattgccgtc cagggtatcc gtctggccgt
     1921 acccggctcc gtagccgtcg ttcggcagaa gccatcccag cggcatgtca tgggccttgt
     1981 aacggtccac gacggcgcgg ccggaaaact ggtaattatt caattccccg ttcagggatt
     2041 ccttaatgcc tccgttatcc ttctggcttt ccttgtaccg tttcccgtcc tcaaacagga
     2101 tgcctttttc atcttccttc cagtaatccc ggttataggc gttcagatgc ccctggtaaa
     2161 aggcgaactt gggcagcaga acgggagcgc cggtaagctg gtagaaatcc cttaaaagac
     2221 tgaccggccc gtcgttgacc atgaaaaaga catccagata attttcatca tgcgagaggt
     2281 tcaccagatt ctcttccctg gaaccgaaat catactgccc tttcttgaag gtatgccaca
     2341 tgacgccgta cccgccggtg gaccagtaaa agggagtggg ggaagccacg ccgccatccg
     2401 tccagctgtt ctggttttct atggaaatga cctttcccct gtgggaaaaa cggccgttct
     2461 gcacgccgcc gccgtaaaaa tattcgtccg gcttcgcctt cagggaaaag ctggttttgc
     2521 ccttttcaaa aaggatgggg gatgcctgct ccgccaccac ggaatgatcc ttcagattga
     2581 tcaccttgaa aagagaggtc tttttactta tctcaatcct gaccttcccc gtagtgatga
     2641 caaccgcatc ccccttctgg tccacatcca gccgggaaac cggcttccgg ggattgtccg
     2701 ccagaatgcg ggcttccggt tccgccttgg ggtcgcggat aataccgcct ttgtcatctc
     2761 tgaaaatacg gaaaatgtta tccccgtaaa aatccagcgt catccggcgg ttgttgtcaa
     2821 gcagcacttc cacggcggca ggattgattt ttctggcgcc ttcaacggcc tggacctgtt
     2881 gtccaggaac cggctcttcc aatgcggcaa gtgcgaagcc cgagaaagaa atggacaatc
     2941 cgaaagccag caggacggaa ttccggaaca tgccatggtc tattgagttt cccatcattg
     3001 tagtgttcta tattattcag cggagcggca ttgcggagag gatgcaaaca gtcgtttccc
     3061 gacgggcagc agaccgcttt cattcattgt gccgtctaat ggcgcaggtt atcttgccgc
     3121 accataccct ttgtccgtaa tcgtaacaag gtcaaagacg gcagtaacgg cgcgccattg
     3181 ctccacacgt ttataaaata cctgcccaca aaaggttacc tttccatgat gacattattt
     3241 cttctccacg gcatcacatc gtcaaatgaa tcaagcgcgc tgaagccctt acggtaaccc
     3301 ctggcgtata ttcccccggc accgggaact gcccgcctgc gcggcggaat gccgcggaca
     3361 acagaacagc aggaaataat atgcgattcc tgtttattgc cgttttgaac agaagattat
     3421 gttctgcatt cccggaccgg caattaatcc ggagcggacc gttccaagaa gaacgccgca
     3481 ggccatccgg gaacaggggg cgtcacgctg acagtctgaa ctcattcggg gaacatcccg
     3541 tattcttctt gaacatgcgg ctcaggtgat ggggatactt gaaccccagt tcataggcga
     3601 tttcactgac ggatttattg gtggcgcaga gaagctcctt ggctttgtcc accaacttga
     3661 gctggatatg ctcctggggc gtttttcccg tctctttctt gacgagatcc ccgaaatagt
     3721 tggcggagag atgcagttca tccgcgaaat actgcacata gggcaacccc tgttcctgag
     3781 gcttgtctcc ttcgaagtag gaatccagca ggcgttcgaa ctccgtcagc acatccctgt
     3841 tcacatgctc gcgggtgatg aactggcggt cgtaaaaacg catgcaatgg ttcagaagga
     3901 cttctatatt ggccgtcacg atggacttgg tgtgccggtc aacgccacgt tccagttccg
     3961 cccggatttc cgtcaggccg ttcaggaaca gatgcctttc cctctccgac atatggaggg
     4021 actcggaagc gtcataggaa aaaaaggtgt aatccctcat cttgcgcccc agggaagtgc
     4081 cgcggagcaa atcgggatgg aacaggagcg cgtacccttc tatccggaaa tattccccgt
     4141 tgtcatcatt gcccagcacc tggccgggag acacgaagac aagagtgccc tcctggtaat
     4201 cataataatg ccttccgtac cgcaggtctc cgcatttcag gtccttcaga aaaacggcat
     4261 aaaacccgta agccttgcgg acatgccgca gcggcgggac cctggaaaaa tcaacgacgc
     4321 tgaccagggg gtgcagcgtt tcctgcccca aaaaggagtt atagtcccct acggaatcca
     4381 tgttcagaat aatgctcatg ccctttctcc tgcttttgac cgaagcatag caggcagggc
     4441 cccggctatc cagttcattc cggcaacctg taatcatggt aggaaaacct gtaatccgtc
     4501 tatcggaaaa aatagaaatc ctatggtagt tttgcaggca tggaaacaat taacgggcaa
     4561 aacatgccat gcagggagct gggcaaaagc ggcctgaagg tttcagccat aggcctgggc
     4621 tgcatgggaa tgagccatgc ctacggagcg cccgcagaca aaaaggaaat gacggaactc
     4681 ctggcccggg ccgtggatat gggatataca ttttttgata cggcggaagt gtacggaacg
     4741 cacgggtgcc ctcacgacaa cgaagaactg ctggggaaag ctctggcgcc ctaccgggac
     4801 aaaatcgtta ttgcgaccaa attcggaatc cgcttcagcg gcacggcagg aaccgggccg
     4861 tccccgctga tcacagattc ccgccccggc acaatccgcc aatccgtgga aggttccctt
     4921 aaaaggctgg gagtagaacg catcggcctg tattaccagc accgcatgga tgattccgtg
     4981 ccgccggaag aagtggcggg cgtcatgcag gagttgatcc gcgaaggcaa aatcgcgcat
     5041 tggggtattt ccgaagcaac ggaggaagcc atacggcgcg ctcacgggac atgtccgatc
     5101 accgccgtcc agaaccgtta ttccatgatg gcgcgccatc atgaatccct ttttcccgta
     5161 ctggaagaac tgggcatcgg ctttgtggcg ttctcccccc tggccaacgg cctgctttcc
     5221 ggccgctacg accagaattc cacatttgag ccgggtacgg actaccgcag ctccatgcct
     5281 cagttcagac cggaaagctt tgacgccaac
//
LOCUS       DAOMSU010000150         5264 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1403_length_5264_cov_2.574390, whole genome shotgun sequence.
ACCESSION   DAOMSU010000150 DAOMSU010000000
VERSION     DAOMSU010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1403_length_5264_cov_2.574390"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            86..376
                     /locus_tag="PK077_07085"
     CDS             86..376
                     /locus_tag="PK077_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24104.1"
                     /translation="MSSNPLKAVGGALANIATFGGYGANKAAKKQASAANAMADAMAN
                     APEQKVITTETKDVSQAEDAVNSSARRRLKLSNTTNRSNPLSSLAGLRKTLG"
     gene            395..2062
                     /locus_tag="PK077_07090"
     CDS             395..2062
                     /locus_tag="PK077_07090"
                     /inference="COORDINATES: protein motif:HMM:NF023657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HRN24105.1"
                     /translation="MENVKDLLRTADALFTEMNKNSGDWDELRRRIMPRMEGKARQQE
                     QANEMTAASSFSPVAHKSLLNLASAHLLFITPMDQKWFSLRPQEERDDYTDEDDWYSK
                     ATEAVYRALADSNFYAAAHEVYLDRCLTGTGCMFADVSRDGSLVFKHVPTGTYAIAEG
                     AHGEVNTLVRTLKFTAQQAVEMFKLGNLPVKIQEAYKNAERRYTEMFEFVHLVLPNSR
                     SQFGSDMVRPGRRKWLDVYIAREAEKIVFHGGFYEFPFLVTRFLKGGVSSYGEAPGKA
                     VLPEIKATLLMDRVMDVAGSRAAIPSVIVSAKMAKEVDLRAGGKTVVPDELIGSQLPR
                     EWANVGDVRFMLERQDKKEKLIREAFFNDILQVVSSVDREMTATEVNARESERIICFF
                     SSFIQFSQDFQTMMNRIVCLMFRNTQGAVLPGDAPDEFFVRSADGGKFELRTPRTRYL
                     GKIAQAFDRLQRYGLEGVLNGLAKYIQVSGDTRIAKRMKAWEVLRFMWDSSGAPSKCI
                     VSASENSKMVEEEKAQEDQMRQAALAEQLARAGKDSAAASAQFNTDS"
     gene            2068..2319
                     /locus_tag="PK077_07095"
     CDS             2068..2319
                     /locus_tag="PK077_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24106.1"
                     /translation="MFEDKPTPEQVEFLKRLNRRRAALKEAFTPEVLDILEKEFQTNL
                     PCFQGKAGSYDPLDAMRRDAQREMLLWVKYEIEQYNPDL"
     gene            2316..3059
                     /locus_tag="PK077_07100"
     CDS             2316..3059
                     /locus_tag="PK077_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24107.1"
                     /translation="MIYNRLFHNRFLREEAIPGSEGEGPGGGAPPPASPVDSPPPANP
                     PVPSNPYDFSGGAEQPDPDPGSPPPLSPQEETEYEIDFGEGFVENDALRDMLKGHARA
                     AGLPADAAGKFLSEVAASIRADEEAAFKEADEALKDEWGAEYETNVSAAKAFARKLSV
                     ESGVSMEKMAVFASPDGFRVLHAISRLTGEGGLKGGGQIPAKTDPADEAQAILSDPNH
                     RYYKAIADPSHPQWREATDYYNKLVGISG"
     gene            3169..3738
                     /locus_tag="PK077_07105"
     CDS             3169..3738
                     /locus_tag="PK077_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24108.1"
                     /translation="MMPQMDKVTVFNQALAQFGDREYVKGSPAGRTVDLWWPTVLREA
                     LLFGAWTWATKRVEMDRSVMRHPIPDDCLRVLYVGADLFRIEGRDLVVERYGKRAAGT
                     DKLVVDYLSDEVARSEVLPDHSPFFIKGVVFLLAGRCALKLASSPQLAAALEAQGEAF
                     LSKALYWDTCQHSSNDQDPLTEILSSSIF"
     gene            3743..4438
                     /locus_tag="PK077_07110"
     CDS             3743..4438
                     /locus_tag="PK077_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24109.1"
                     /translation="MSSDFGVSQQYKYQGQAALSNGRATQAAYEKKARALEAEAVSDS
                     HLAARNMKRMRQNQNAAMGSVRAQRGGSGFTSEGSGSQAEVAVADVWESAIGDAALSN
                     AVSDANKRFAAESARYQGDLAMMAARSEADQYKMLSQNALGSAMIQTALTVAGGVMGA
                     AGMSGGGLLGGVTESGDEWGAKVGGTQGAFSGMMNAYSLSGSLGGMVPGSMQSSNRLR
                     DSLLANFMGFGKR"
     gene            4435..4776
                     /locus_tag="PK077_07115"
     CDS             4435..4776
                     /locus_tag="PK077_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24110.1"
                     /translation="MSVSPMQQAFFLMEAQRPGWFRETVSLADAGGGIVWCCPSLFFA
                     GVPDPESPRTLIVLFAHGRMEAVRELACLVQGRFDRARWQRCIRGREDWKEISITRFL
                     SFNRFKMKEDE"
     gene            4769..>5264
                     /locus_tag="PK077_07120"
     CDS             4769..>5264
                     /locus_tag="PK077_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24111.1"
                     /translation="MSDLQQPMYGGARMNAASSTPAPVQMPDVSSKPVQRALQNAQEF
                     VSDVAHQYQCMKDFGEQTRLEGRMNDLASEFEQEMTRRLGFARGHELSFYDRDGRLKE
                     SALNTFVRNYEGKFRELKGSFVSQEEAARFGARQQDVMRRLQGRASELMLKGQIQESR
                     QAFEE"
BASE COUNT         1031 a         1227 c         1765 g         1241 t
ORIGIN      
        1 gtgttcggga agggctggct gaaccgctgc gacgatgcgt tcagcgtggc gcagaagctt
       61 gtttagttgt tttccattag ttgttatgag ttcaaatcca ttaaaagctg tcggaggggc
      121 cctggcaaat atcgccacgt tcgggggata tggagccaat aaggcggcca agaagcaggc
      181 aagcgccgcc aacgctatgg ccgatgccat ggcgaatgcc ccggagcaga aggttattac
      241 tacggaaacc aaggatgttt cccaagcgga ggatgcggtg aattcgtctg cccgccgccg
      301 cttgaagctt agtaatacga cgaaccggag taatcctctt tcttccctgg ctggcctgag
      361 gaagacgctg ggttgatttt tacacaggag attcatggaa aatgttaaag atttattgag
      421 gacggcagac gccctgttca cggagatgaa taagaattcc ggggattggg atgaattgcg
      481 ccggcgcatc atgccgagga tggaggggaa ggcccgccag caggaacagg ctaatgagat
      541 gacggctgcg tccagttttt ctccggtggc gcataagtct cttttgaatt tggcgtccgc
      601 tcatcttctt tttattactc ccatggatca gaagtggttt tccctgcggc cgcaggagga
      661 aagggatgat tacaccgatg aggatgactg gtacagcaaa gcgacggagg ccgtctaccg
      721 tgcgctggcg gattccaatt tctacgcggc ggcccacgag gtttacctag accgttgcct
      781 gacgggtaca ggctgcatgt ttgcagatgt ttcccgtgac gggtccctgg tgttcaaaca
      841 cgttcctacc gggacttatg cgattgccga gggagcccac ggggaggtga atacgctggt
      901 gcggacgttg aagtttactg cccagcaggc cgtggagatg tttaagctgg gtaatctgcc
      961 tgtcaagatt caggaggcgt ataagaatgc ggagaggcgg tacaccgaga tgtttgagtt
     1021 tgttcacctt gtactgccca acagccggtc gcagttcggt tccgacatgg taaggcctgg
     1081 ccgccgcaag tggttggacg tgtatattgc cagggaggcg gagaagattg ttttccacgg
     1141 cggcttttac gagtttcctt ttctggtgac gcgctttttg aagggcggcg tttcttctta
     1201 cggcgaggct ccgggcaagg ctgtgctgcc ggagatcaag gctaccctgc tgatggatcg
     1261 ggtgatggat gtggccggca gccgggcggc cattcccagc gttatcgtgt cggctaagat
     1321 ggcaaaggag gttgatttgc gggccggagg caagacggtt gttccggatg agcttattgg
     1381 ttcacagttg ccgagggaat gggcgaacgt gggggatgtg aggtttatgc tggagcggca
     1441 agataagaag gagaagttga tcagggaggc gtttttcaat gatattctcc aggtggtttc
     1501 aagcgtggac cgcgagatga cggctacgga ggtgaatgcc cgcgagtcgg aacgcattat
     1561 ttgctttttt tcttctttta ttcagttttc gcaggatttt cagacgatga tgaatcgcat
     1621 tgtctgcctg atgttccgca atacgcaggg ggccgtgctt ccgggcgacg cgcccgatga
     1681 gttttttgtc cgttccgccg atggggggaa gtttgagttg cggactcccc gcacccgtta
     1741 tctgggcaag attgcccagg catttgaccg tttgcagagg tacggccttg agggggtgtt
     1801 gaatgggttg gcgaagtata tccaggtttc gggcgatacc cgcattgcca agcgcatgaa
     1861 ggcatgggag gtgttgcggt ttatgtggga cagttccggc gccccgtcca agtgcattgt
     1921 gtccgcgtcc gagaatagca agatggttga ggaggagaag gcgcaggagg atcagatgcg
     1981 tcaggccgcc cttgcggagc aattggccag agccggcaag gatagcgccg cggcgtccgc
     2041 acagtttaat acggattcat gatgaatatg tttgaagata agccgacacc ggaacaggtt
     2101 gagtttctca agaggctcaa ccggaggcga gccgcgctga aggaggcttt tactccggag
     2161 gtgctggata ttttagagaa ggagttccag acgaatttgc cctgctttca agggaaggct
     2221 ggttcctacg acccccttga cgcgatgcgc cgagacgccc agcgggaaat gctcctgtgg
     2281 gtgaaatacg agatcgaaca atataaccct gatttatgat atacaataga ttattccaca
     2341 ataggttcct gagggaagag gccattcccg gcagcgaagg tgaaggcccc ggcggcggag
     2401 cgccgccccc ggcaagtccc gtggacagtc cgcctcccgc gaatcctcca gtcccgtcca
     2461 atccctacga tttttcaggg ggtgcggaac agcccgatcc ggatcccggc agtcctcccc
     2521 cgctttctcc gcaggaggag accgagtatg agattgattt tggagagggg tttgtggaga
     2581 atgatgccct gcgagatatg ttgaagggtc atgccagggc ggcagggctg ccggccgatg
     2641 ccgccgggaa gtttctttcc gaggtggccg ccagcatccg cgcggacgag gaggctgctt
     2701 ttaaggaggc tgacgaggcg ttgaaggacg aatggggagc ggagtatgag acgaatgttt
     2761 ctgccgccaa ggcgtttgcc cggaagcttt ccgtggagtc cggcgtttct atggagaaga
     2821 tggctgtgtt tgcgagtccg gacgggttcc gcgttctgca cgccatttcc cggctgacag
     2881 gcgagggagg cttgaagggc ggcggccaga ttccggcgaa gacggatcct gccgacgagg
     2941 ctcaagctat tttgtccgac cccaatcacc gttattataa ggcgatcgcc gatccttcgc
     3001 atccacagtg gcgggaggct accgattatt ataataagct ggtggggatt tccggttagt
     3061 tttttgcgtt gactattggt tcggaggggt gtcctgctgt gcggggcgcc ctttcttttt
     3121 ttcatttgtt caagttacgg ttgtattcat caggcctggg gatgtggcat gatgcctcaa
     3181 atggataagg tgaccgtttt taaccaggct ttggcccagt ttggggaccg ggagtatgtg
     3241 aagggttccc cagccggtcg caccgttgat ttgtggtggc ctaccgtgtt gcgggaagcg
     3301 ctgttgttcg gggcatggac ctgggcaacc aaacgtgttg agatggatcg ctccgttatg
     3361 aggcatccga ttccggatga ttgcctgcgc gtgctgtatg tgggggcgga tttgttccgc
     3421 attgaggggc gtgatttggt ggttgagcgt tacgggaagc gcgccgccgg gactgataaa
     3481 ctggtggtgg attatctttc cgacgaggtg gcccgttccg aagtgctgcc ggatcacagt
     3541 ccgtttttta tcaagggcgt tgtgtttctt ctggcgggca ggtgcgcttt gaagctggct
     3601 tcttctcccc agcttgcggc cgctttggag gcacaggggg aggcgttttt aagcaaggcc
     3661 ctttattggg acacctgcca gcattcttcc aatgaccagg atcctttaac agagatttta
     3721 agcagttcca ttttctgatg ttatgagttc cgatttcggg gtttcccagc agtataagta
     3781 tcaggggcag gcggctttga gcaacgggcg cgccacgcag gcggcttatg agaagaaggc
     3841 ccgcgccctg gaggcggagg cggtttccga ttcccacctg gccgcccgca atatgaagcg
     3901 gatgcgccag aatcagaatg ccgccatggg gtctgtacgg gcacagcgcg gcggatccgg
     3961 ttttacttcc gagggttccg gcagccaggc ggaggtggcg gtggcggatg tgtgggagag
     4021 cgccattggg gacgcggccc tttccaacgc tgtttccgat gccaataagc ggtttgccgc
     4081 ggagtccgcc cgataccagg gggatctggc catgatggcg gcacgcagcg aggcggacca
     4141 gtataagatg ctttcacaga atgcccttgg ttctgccatg atccagacgg ccctgacggt
     4201 ggcggggggt gtcatggggg cggcaggaat gtccggtggc gggttgctgg ggggtgttac
     4261 cgagagcggg gatgagtggg gagctaaggt gggaggaacc cagggggctt tttccgggat
     4321 gatgaatgct tattcccttt ccggttccct gggggggatg gtgccgggga gcatgcagtc
     4381 ttccaacagg ttgagggatt ccctgctggc taatttcatg ggttttggaa agagatgagc
     4441 gtttctccca tgcagcaggc tttttttctg atggaagccc agcgcccagg ctggttccgg
     4501 gagaccgttt ccctggcgga cgcgggagga gggatcgtgt ggtgctgccc ttcgttgttt
     4561 tttgcggggg tgccggatcc ggagtctccc aggacattga ttgttctttt tgcccacggc
     4621 cgcatggagg ccgtcaggga gctggcttgt ctggtgcagg ggcgttttga ccgggcaagg
     4681 tggcagcgct gcatccgcgg gcgcgaggac tggaaggaga tttccatcac caggttttta
     4741 agttttaacc gtttcaagat gaaagaagat gagtgattta cagcaaccca tgtacggagg
     4801 agcccggatg aatgcggctt cctccacccc tgccccggtc cagatgccgg atgtttcttc
     4861 caagcccgtt cagagggcgc tgcagaatgc ccaggagttt gtgtctgatg ttgcccacca
     4921 gtaccagtgc atgaaggatt tcggcgagca gacgcggctg gaaggccgga tgaatgattt
     4981 ggccagcgag tttgagcagg agatgacccg gagattgggg tttgcccgcg gtcatgagct
     5041 gtctttttac gatcgtgacg ggaggctgaa agagagcgcc ctgaatacgt ttgtacggaa
     5101 ttacgaaggg aagttccgcg agttgaaggg gagttttgtt tcccaggagg aggccgccag
     5161 gttcggagcc agacagcagg atgtgatgcg ccgactccag gggagggctt ccgagttgat
     5221 gcttaagggg cagattcagg agtccagaca ggcttttgag gaag
//
LOCUS       DAOMSU010000151         5163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1453_length_5163_cov_2.989233, whole genome shotgun sequence.
ACCESSION   DAOMSU010000151 DAOMSU010000000
VERSION     DAOMSU010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5163
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1453_length_5163_cov_2.989233"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PK077_07125"
     CDS             complement(<1..1101)
                     /locus_tag="PK077_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRN24112.1"
                     /translation="MFLEKNSYGYGGHNFFDYKPIDDLELVKRGYAAVFISMEDLYGS
                     PKAMEVMDQFYRYLVDERKFSGKPVLFGLSRGGLYALNWAEKNPLCVAGVYVDAPVCD
                     FKSWPAGRGKGKGSPDDWNKCLRAYGFNEQQALSYKGNPVDNMRGMAKAGIPLLFISR
                     TEDDVVPIEENTDVFAKRYAKLGGPVKVIRRPGGHHPHGFDNPAHIVDFVLSVTGQPV
                     PLRVACLGDSNTFGAGVAGANKDVLRWSHLLGQELRKKFKRKEIEVLNCGVNGRTLLA
                     KGDLPYIKTGEYRNALNSHAQIAIIALGTNDAKPQNRKFLSEFKQNYAAIIAELRENM
                     PGIQIYCAIPLPSWQSSSQIDKETVETKIIPLV"
     gene            complement(1572..2027)
                     /locus_tag="PK077_07130"
     CDS             complement(1572..2027)
                     /locus_tag="PK077_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24113.1"
                     /translation="MLLCCAGVGMRLHAQPLNKGRIAILGDSIAYAGPWANEVENALK
                     ADKKFEACEIVNFAVPSETVAGLSEYGHAGGRFPRPCLHECLDRVLQMYRPQLILACY
                     GMNDGLMQAFDKARFQAYQEGNIRLKKAADAAKAEIVFITPPLFRGGFR"
     gene            2436..>3537
                     /locus_tag="PK077_07135"
     CDS             2436..>3537
                     /locus_tag="PK077_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase III"
                     /protein_id="HRN24114.1"
                     /translation="MHTNPPSDIGNAATFIAEIYGQDVFNMETMRNYLPRPVYKKLLA
                     TIRKDEKLDPDIANEVAQAMMTWALEKGASHYTHWFQPLNGSTAEKHDSFVDVDPEGN
                     LELKFSGKSLVQGEPDASSFPSGGLRATFEARGYTAWDPTSPAFIKRTENGATLCIPT
                     AFCSYKGQALDKKTPLLRSMTAVTRQAKRLLSCFGGPDNIHVSADLGAEQEYFLVDKQ
                     LYALRPDLMMCGRTLFGNVPPKHQQMEDHYFGSIKDRVLEFMVDVDEALWKLGIPAKT
                     RHNEVAPGQFEIAPIFESQNLAVDHNMLVMEVLRKTANKHDMVCLLHEKPFSGMNGSG
                     KHNNWSLSTDTGVNLLSPGETPYENAQREQVDA"
     assembly_gap    3538..3547
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3548..4651
                     /locus_tag="PK077_07140"
     CDS             <3548..4651
                     /locus_tag="PK077_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase type III"
                     /protein_id="HRN24115.1"
                     /translation="NNWSLSAPGYGSLLNPGSSPQENAIFLTLLCATIKAVDEHADLL
                     RASVAKSGNEHRLGAHEAPPAIISIFLGDLLDEIIEQIEKGGTKKARTQKTINIGVDT
                     LPMFPLDASDRNRTSPFAFTGNKFEFRAVGSSQTCAWPMTVLNTIVAESLDEICTILE
                     PVKDKPEEFHATLNKLLQNIIKKHKRILFSGDGYGEAWVEEAERRNLPNIPGTIEALA
                     ALETPKAKALFEKYKVVSPVELHARHEIQTEIFHKEINIEAETALLMVETLYIPAVTE
                     QLTQLTTAIAQMKASGIKAGMKATTDRSNRIGTLLDLLPGQVRELRRAVDEADTKAIQ
                     AGMDNLRSTIDMLESLTDANLWPVPTYAELLFL"
     gene            4862..4990
                     /gene="rpmH"
                     /locus_tag="PK077_07145"
                     /pseudo
     CDS             4862..4990
                     /gene="rpmH"
                     /locus_tag="PK077_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
BASE COUNT         1291 a         1559 c         1210 g         1093 t
ORIGIN      
        1 cacaagagga atgatcttag tttccaccgt ttccttgtca atttggctgg aagattgcca
       61 cgagggaaga ggaattgcgc aatagatttg aatgccgggc atattttccc gcagttcggc
      121 gatgatggcg gcataattct gcttaaactc ggacaggaat ttcctgttct gcggtttggc
      181 gtcattggtg cctaaagcga taatggcaat ctgtgcatgg gaattgagag cattccggta
      241 ctcgcctgtt ttaatatagg gaaggtctcc cttggccaga agggtacgcc cgttaacgcc
      301 gcagttcagc acttctattt ctttccgttt gaattttttc ctcaattctt gaccaagaag
      361 atggctccag cgcaaaacat ccttattggc tccggccact cctgctccaa aagtattact
      421 gtctcccagg cacgcaacgc gcaaagggac gggctgtccg gtaacggaaa gaacaaaatc
      481 tacgatatgg gcaggattgt caaaaccgtg cggatggtgc ccgccggggc ggcgaataac
      541 ttttacaggg cctcccagct tggcgtaacg tttggcgaaa acatccgtat tctcttcaat
      601 agggactaca tcatcttccg tccgggaaat aaacaacaaa ggaattccgg ctttagccat
      661 gccgcgcata ttgtccacgg gattgccctt ataggataaa gcctgctgtt cattaaagcc
      721 ataggcgcgc aggcatttgt tccagtcatc cggcgaacct ttgcccttgc ctctgccggc
      781 cggccagctt ttgaaatcac agacgggggc atccacataa acgccagcca cgcacaaagg
      841 attcttctcc gcccagttca gagcataaag tcctccacga ctcagtccaa acaacaccgg
      901 ttttccggaa aatttgcgtt catccaccag atacctgtaa aattgatcca tcacctccat
      961 ggccttggga gaaccgtaaa ggtcttccat gctgataaat accgccgcat agccgcgctt
     1021 taccagttcg agatcatcta taggcttata atcaaaaaaa ttgtggccgc catatccata
     1081 actgttcttt tccagaaaca acagccttgt cgggaaaaac gatgaatgcc ctgcggccgc
     1141 ccacggtaaa ctcctcccgt aaaaaaccat tccagacaga aaccgtccta ggaaactgac
     1201 gcttggccat ttccggatca taccccggaa tttccggccg tgcatgtcct gtttttacca
     1261 gccattcatt gcggactttc tcatgttttt tcatcgtatc cggcgtcggc cgatggtctt
     1321 tttccgcaac ctcaggagca aggcctattt ccgggaccag cccggcaaga acttcccggg
     1381 ctatcatatg gtgcccttcc ggaccggggt gcacgccgtc accggaatac ctgaaaccgg
     1441 gattcccggc cttctctctg gcaatgagct tccgaagccc gggacggata tctatgactt
     1501 tccagccgtc ttttctttta gaaaccagcc attccgcgta cttgtccaat acagcgtcat
     1561 actcagcctg ctcagcggaa tcccccccgg aacaatggag gagtaatgaa gacaatctcc
     1621 gcttttgccg catcggcagc ctttttaagg cggatattcc cttcctggta cgcttgaaaa
     1681 cgcgctttgt caaacgcctg cataaggccg tcattcatcc cgtagcatgc cagaatcaat
     1741 tggggccggt acatttggag aacccggtca aggcactcat gcaggcatgg acgggggaat
     1801 ctgccacccg catggccata ctcgctcaac ccggctaccg tctcgctggg aacggcaaag
     1861 ttgacaatct cgcaggcctc aaacttttta tcggctttca aggcgttttc cacctcattt
     1921 gcccatggtc ccgcataagc aatactgtcc ccaagaatgg caatccggcc cttattcaaa
     1981 ggctgggcat gaagccgcat ccccactccc gcacaacaca gcagcatcaa caataaatac
     2041 cttactatta acaataaacg catttcacaa taatatggtt gtacttatta ctactgaaaa
     2101 cgaaatagat ttcttgcaag atacggaaaa ataccgctcg gcaggttcct gtgccgtttc
     2161 atgaaacaaa aacccggcac ttcaccaatt cccgtccagt cctcttcccg acaatcacgg
     2221 cctatccctt cctttacagc gtccccctcc ttccccaatt tctccattgc cgccggagaa
     2281 ccatagaaac cggaaaaccg tttacgcttc ttcccgaaaa cggatggggg cggaagaagt
     2341 ccttttcctg aagaaatagg gcaggattcg gcttgccaaa aagtctcttt taaggtttgg
     2401 tatgttccga gccttttggc gcgttcccta tcaagatgca tacaaaccca ccttccgata
     2461 ttggcaacgc cgcaaccttt attgcggaaa tttacggtca ggatgtcttc aacatggaga
     2521 ccatgcgcaa ttacctgccc cgccccgttt acaaaaaact gctggctacc atccgcaagg
     2581 acgaaaaact ggaccccgac atcgccaatg aagtggctca ggccatgatg acgtgggctc
     2641 tggaaaaggg ggccagccac tacacgcact ggttccagcc tctcaatggg agcacggcgg
     2701 aaaagcacga ctcctttgtg gacgtggacc cggagggaaa tctggaactc aaattttccg
     2761 gcaagagcct ggtgcagggc gagccggacg cctccagctt cccttccgga ggtctccgcg
     2821 ccacctttga agcgcgcggc tacaccgcat gggaccccac cagccccgcc tttatcaaac
     2881 ggacggaaaa cggcgccacg ctttgcatcc caaccgcttt ctgctcttac aagggacagg
     2941 cgctggacaa aaagacgcct ctcctgagat ccatgactgc tgtcacccgg caggccaagc
     3001 ggctgttgtc ctgctttggc ggtccggaca acattcacgt cagcgccgac ctgggggcgg
     3061 aacaggaata cttcctggtg gacaagcagc tttatgccct gcgcccggac ctgatgatgt
     3121 gcgggcgcac cctcttcggc aatgtgcccc ccaagcacca gcagatggaa gaccactact
     3181 tcggctccat caaggaccgc gtgctggaat tcatggtgga cgtggacgaa gccctttgga
     3241 aactcggcat cccggccaaa acgcgccaca atgaagtcgc tcccggccag tttgagatcg
     3301 cacccatttt tgaaagccag aacctggccg tggaccataa tatgctggtc atggaggtgc
     3361 tccggaaaac tgccaacaag catgacatgg tctgcctgct gcatgaaaag cccttctccg
     3421 gcatgaacgg ctccggcaag cacaacaact ggtccctctc caccgatacc ggcgtcaacc
     3481 tgctctctcc cggcgagaca ccctatgaaa atgcccagag agagcaggtt gacgccgnnn
     3541 nnnnnnnaac aactggtccc tctccgcccc cggttacggc agcctgctca atcccggttc
     3601 cagcccgcag gaaaacgcca tcttcctcac tctgctctgc gccaccatca aggccgtgga
     3661 cgaacatgcg gatctgctgc gcgcctccgt cgccaaatcc ggcaatgaac accgcctggg
     3721 ggcccatgaa gcccccccgg ccatcatttc catcttcctg ggagatctgc tggatgaaat
     3781 cattgagcaa atcgagaaag gaggcactaa aaaagcgcgc acccaaaaga ccatcaacat
     3841 cggcgtggac accctgccca tgttccccct ggacgcctcc gaccggaacc gcaccagccc
     3901 gtttgccttc acaggcaata aattcgaatt ccgcgccgta ggttcctccc aaacctgtgc
     3961 atggcccatg acggtgctca acaccattgt ggcggaaagc ctggatgaaa tctgcaccat
     4021 cctggaaccc gtcaaggaca agcccgagga attccacgcc accctcaaca agcttcttca
     4081 aaacatcatc aagaaacata agcgcattct attcagcgga gacggctacg gagaagcctg
     4141 ggtagaggaa gcggaacgcc gcaatctccc caacatcccc ggcaccattg aagccctggc
     4201 cgccctggaa actcccaagg ccaaggcgct ttttgaaaag tacaaagtgg tcagccccgt
     4261 agaactccac gcccgccatg aaatccagac ggaaatattc cacaaggaaa tcaacattga
     4321 agcggaaacg gccctcctga tggtggaaac cctttacatc cctgccgtaa cggaacagct
     4381 cacgcagctt accaccgcca tcgcccagat gaaagcatcc ggcatcaagg cgggaatgaa
     4441 agctactacg gaccgctcca accgaatcgg cacgcttctg gatcttctcc ccggccaggt
     4501 gcgggaactg cgccgcgccg tggatgaagc cgataccaag gccatccagg cgggcatgga
     4561 caatttgaga agcaccattg atatgctgga aagcctgacg gacgcaaacc tgtggcccgt
     4621 tcccacatat gcggaactgc tctttctgta aagcaggagg gcttttctga aatagaaaaa
     4681 cactgtttat caatcataaa gattacttat cccagtactt tccccaagaa aaacggcaaa
     4741 ttaatcgcct gacggattga caaaacaatc aaaatagtta tcctttccgt ccggttgtgc
     4801 ataggccgct tagacggacg gcactgaatc tagtaattca caagaagcat ctttcaacat
     4861 catgagcaag aggacttatc aaccatccaa gcgttgccgc aagcgtcagt tcggattccg
     4921 tgcacgcatg gccaccaaga acggagcgga catcatccgc cgccgccgcg ctaaaggccg
     4981 caagcgcctg cttcccaagg gagccgaaat ccagtacaag cgccacacca tccagcacgg
     5041 ccgttaatcc agccgccagg atccggttaa cgcctccttg gaactttctt ttcacgggcc
     5101 gacccgcggg agatgcgtct tactcgtcaa caaagcatga ccagggcatt tcaatttgcc
     5161 cgg
//
LOCUS       DAOMSU010000152         5151 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1459_length_5151_cov_2.937794, whole genome shotgun sequence.
ACCESSION   DAOMSU010000152 DAOMSU010000000
VERSION     DAOMSU010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1459_length_5151_cov_2.937794"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(268..972)
                     /locus_tag="PK077_07150"
     CDS             complement(268..972)
                     /locus_tag="PK077_07150"
                     /inference="COORDINATES: protein motif:HMM:NF016152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HRN24116.1"
                     /translation="MFFRRIPRKSWYEKTVERVFRDRKLCVEKLLSFGFVRVESGFLR
                     RAALLDGQFCMELEIHADGSVHATVYDADGKNIRHADPGTEDRLRTRMLRREYEEELW
                     HVAECCFEPDFFKAAPARSLIAHIRKAYGEELEFLWRKFPGNAVVRRKDTEKWYAAFL
                     AVPRLKLGGSSKERVEVLNLRVCPGESGILADNRSRFPAYHMNKKNWVSFCLDGTVPF
                     EELAARLETSRRLAGK"
     gene            complement(1121..1915)
                     /locus_tag="PK077_07155"
     CDS             complement(1121..1915)
                     /locus_tag="PK077_07155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRN24117.1"
                     /translation="MTIPDNSDRPGARIPCETRNVFGFSVAVSSVEEMSAALAERALE
                     AEAPFLVAAADAHVVTLGVHDRDYGNVLERMDVICPDGMPVVWRLNRRLSSGEREACR
                     VSGPDLMEALVRSNVRYPGLRHFLLGGDEKLLEALSGALKEKYPGFQLAGAYSPPFRP
                     WTEEDLKNMREAVASSGANVVWVGLGCPKQERWMAEQRELLPPAVYVGVGAAFAFHAG
                     TVKRAPRWMQKNGLEWLYRIYREPGRLLRRYVKHNSLFVWYVLTGR"
     gene            complement(1938..2711)
                     /locus_tag="PK077_07160"
     CDS             complement(1938..2711)
                     /locus_tag="PK077_07160"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRN24118.1"
                     /translation="MNILFLGTGTSTGVPQIGCSCAVCTSPDPRNRRLRSSIYVEAAG
                     TRLLLDSSPDLRQQALRENITDVDAVLYTHAHVDHVGGFDDLRAFCWRRSGGLPMYAS
                     PMTVDALRTMYGWAFVPKPGRSGYVRPEPHEVTAPFRVGNVLATPLPVLHAGVETYAY
                     LLEAEGRSLVYMPDVKSIPAPSLERMKGVDLLIIDGLRYHLHPTHMCLEETLAAIAAV
                     LPRRAVLTHLSHDMDYGILSGKLPESVMPAYDGLRLSLP"
     gene            2776..4434
                     /gene="recN"
                     /locus_tag="PK077_07165"
     CDS             2776..4434
                     /gene="recN"
                     /locus_tag="PK077_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRN24119.1"
                     /translation="MLTLLKIKNLALVDQLLWEPSSGFICITGETGAGKSVIIGAIRL
                     ALGERADKTLIRSGEQQCSVEAVFHLPESSPVHAILNEHGVPPCEDGNLIIKRLISST
                     ANRQFLNDSPCTLNLLREAGACLVDMHGPSDHRSLVSQERQLSLLDAFGEHAPLVHAY
                     SDAWRQWQDARRAYDDLEHAEAATAREIELLRHQVDEIDSAAFTPEEVLTLEERWQRA
                     RNGTRLREQVSKMLSMLEETDVPGLGTQLRELTRAAHELERMDASTAAWLAPLAGVNL
                     ELKEIEGRLADYSAELDCDPRELFQLEERINLLESLKMKYGPSFEDVCSRREEAASRL
                     DRIEHRTERLEELRASIAVLRKQMDAAGQALTRARQDSAPRLAASIVRHSRELGFRQA
                     VFEVSLSPLQEPGSQGMETVEFLFGPNPGEPSKPLRLIASSGELARIMLAIKSALAHK
                     DATPLLVFDEIDANVGGEVARAVGFKMQQLGNRHQVISITHFPQVAALASHHYLVQKA
                     SAGNRTISCLREVSHEERVDELVRMLGGGGDHARTLAQALLQPS"
     gene            complement(4465..>5151)
                     /locus_tag="PK077_07170"
     CDS             complement(4465..>5151)
                     /locus_tag="PK077_07170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRN24120.1"
                     /translation="GISWKEDESRLREFMRQGASPLYVGRGGCPAGVIMVADSLKPDS
                     RAAVDSLKRMNLRVVMLTGDNAATARHMAGELHIDEVVSDVLPDEKASHVMKMEERGD
                     KVAMVGDGVNDAPALACARVGISMKSGTELAMESSDIVLMKSNPAGVAEAVQLGRTTL
                     LIIRQNLFWAFFYNIIGIPLAAGCLYPAFGLTLNPMIAAGAMSLSSLCVVFNSLRLRG
                     FTPRLGSLGK"
BASE COUNT         1100 a         1694 c         1441 g          916 t
ORIGIN      
        1 cgtaaagggc gccgcacagg ccgtctggag cctttccggt accgcaggag gaaacggttt
       61 ccagcaaatc ctccctgccc agggccgcgc agacggcctg ggcgcagttg tggcgccagg
      121 gcgccgtgcg gaaggtggac agagcttctt cttctatgtt catgccctta ctgtaggctc
      181 cgcctctttc cgggtcaaga ccccctttcc ggccgtccat ccgggggcgg gaagacggtt
      241 tctcccggca tcccgcgccg gaggcgctca ctttccggca agcctccggc tggtttccag
      301 gcgcgcagcc agttcctcaa agggaacggt tccatccaga cagaagctca cccagttttt
      361 cttattcata tggtaggccg gaaaacggct gcgattatcc gcaagaatcc cggactcacc
      421 gggacaaacc cgcaggttca aaacctccac ccgttccttg gaactgcccc ccagcttcaa
      481 gcgcggcaca gccagaaaag cggcgtacca tttttccgta tccttccgcc gcaccactgc
      541 gttccccgga aacttcctcc acaggaattc cagttcctcc ccgtaagctt tacggatgtg
      601 cgcgatgagg ctccgggcgg gagccgcctt gaaaaaatca ggttcaaagc agcattccgc
      661 cacatgccac agttcttcct catattccct gcggagcatt cttgtccgca acctgtcctc
      721 cgttcccggg tcagcatgcc ggatattctt cccgtccgca tcatacaccg ttgcatgaac
      781 agaaccgtcc gcgtgtattt ccaattccat gcagaactgg ccgtccagca atgccgccct
      841 acgcagaaaa ccgctttcca cacgaacgaa accgaaggag agcagctttt ccacgcacag
      901 cttcctgtcc ctgaaaacgc gttccacagt tttttcatac cagcttttcc ggggaatacg
      961 gcgaaagaac ataagaaaaa caaggttaaa gacgaaggag ggaagagcgc ccaccacttc
     1021 cgtccaggtt caaacggaat catgataggg ccgcacgaaa acggcacaag gataaaataa
     1081 cccgcggctc aactccgggg cgcacaccgg aaaaacggcc tcatctgccc gtcagcacgt
     1141 accacacgaa caggctgttg tgcttcacgt accgcctgag cagtcttccc ggctcccggt
     1201 aaatgcggta caaccattcc agaccgttct tctgcatcca tcgcggagcc cgtttcaccg
     1261 taccggcatg gaaagcgaac gccgccccta cgcccacata aaccgcgggg ggcagcaatt
     1321 ccctctgctc cgccatccac cgctcctgtt tcgggcagcc cagccccacc cataccacat
     1381 tggccccgct tgaagcaacc gcctcccgca tatttttcag atcttcctcc gtccaagggc
     1441 ggaacggagg ggaatacgcc ccggccagtt gaaaaccggg gtatttttcc tttaatgccc
     1501 cagacagggc ttccagcagc ttttcatccc cgcccagcaa aaaatggcgc aagcccggat
     1561 accgcacatt cgaacgcacc agcgcttcca tcaaatccgg cccgctcacg cggcacgctt
     1621 ccctttcccc ggaagaaagc ctccggttca gcctccatac taccggcata ccgtccgggc
     1681 aaatcacgtc catccgttcc agcacgttcc cgtaatcccg gtcatgcacg ccgagggtta
     1741 ccacatgggc atccgccgcg gccaccagaa acggagcttc cgcctccagc gcgcgttccg
     1801 ccagcgcagc ggacatctct tccacggaac tgacggctac ggaaaagccg aatacgtttc
     1861 tggtttcgca cggtatccgc gcgccgggac ggtcagaatt gtctggaata gtcatctgag
     1921 aacatgcagc gtgagcatta cggcagggac agcctcagcc cgtcatacgc gggcatgacg
     1981 ctttctggca gcttgccgga aagaatcccg taatccatat catgggacag gtgggtcagc
     2041 acggcgcggc ggggcaggac ggcggcaatg gcggccaacg tttcctccag gcacatgtgc
     2101 gtggggtgca gatgataacg cagtccgtca ataatcagca aatccactcc tttcatcctc
     2161 tccagggacg gggccgggat gcttttcaca tccggcatgt aaaccaggct ccggccttcc
     2221 gcttccagga gataggcata tgtctccacc ccggcatgga gcaccggaag gggcgtcgcc
     2281 agcacgttcc ccacacggaa cggagccgtc acctcgtgag gctccggcct gacgtagccg
     2341 ctccgccccg gtttaggaac gaacgcccag ccgtacatgg tccgcagggc gtccaccgtc
     2401 atgggagaag catacatggg caggccgccg gaccggcgcc agcagaaggc gcgcaaatcg
     2461 tcaaaacctc ccacgtggtc cacatgggca tgcgtataaa gaacggcatc cacatccgtg
     2521 atattttccc gcagggcctg ctgccgcaaa tccggggaag aatccagcag caggcgggtg
     2581 ccagcggcct ccacataaat tgaagaacgc aaccgcctgt ttctggggtc cggggacgtg
     2641 cagaccgcgc aggagcagcc tatctgcggc actccggtgg acgttcctgt gcccaaaaag
     2701 agaatgttca tcacagaggt catgattgcg tttagggcaa atgatgccat aatgcattca
     2761 aaatccaaag cccgaatgct tacattgctg aaaattaaaa acctggccct tgtggaccag
     2821 ctgctctggg aacccagttc cggattcatc tgcatcacgg gggaaaccgg ggcggggaaa
     2881 tccgtcatca ttggagccat acgcctggcg ctgggagaac gggcggacaa gacgctcatc
     2941 cgctccgggg aacagcaatg cagcgtggaa gctgtgttcc atctgccgga atcctccccc
     3001 gtgcacgcca ttctgaacga gcatggcgtc ccgccctgcg aagacggcaa cctgattatc
     3061 aagcgcctca tctcttccac ggccaaccgc cagttcctga acgacagccc gtgcaccctg
     3121 aatctgctga gggaagcggg agcctgcctg gtggacatgc acggccccag cgaccaccgc
     3181 tccctggtct cccaggaaag gcagctttcc ctgctggacg ccttcgggga gcacgctccc
     3241 ctggtgcacg cctattcgga cgcctggagg caatggcagg atgcgcgccg ggcctatgat
     3301 gacctggaac atgcggaagc ggccaccgca cgggaaatag aactgctgcg ccaccaggtg
     3361 gatgaaatag attccgccgc cttcactccg gaagaagtgc tcacgctgga agaacgctgg
     3421 cagcgcgccc gcaacggcac ccggctccgg gaacaggtct ccaaaatgct ttccatgctg
     3481 gaggaaacgg atgtgcccgg cctggggacc caattgaggg aattgacacg ggcggcccat
     3541 gagctggaac gcatggacgc ctccaccgcc gcgtggctgg ctccattggc gggagtgaat
     3601 ttggaactca aggaaattga gggacgcctg gcggattatt ccgccgaact ggactgcgac
     3661 ccccgggaac ttttccagct ggaggaacgc atcaacctgc tggaatctct gaaaatgaaa
     3721 tacggcccct cctttgagga cgtctgctcc cggagggagg aggcggccag ccgcctggac
     3781 cgcattgaac accgcacgga acgcctggaa gagctgaggg cctccatcgc cgtcctgcgc
     3841 aagcagatgg acgccgccgg gcaggctctg accagagcgc gccaggactc cgcgcccagg
     3901 ctggcggctt ccatcgtcag gcactcccgg gagctgggat tccgccaggc cgtctttgaa
     3961 gtgagcctct ccccccttca ggaaccgggc tcacagggaa tggaaacggt ggaattcctg
     4021 tttggcccca atcccggaga accctccaag cctctccgtc tgattgcctc cagcggggaa
     4081 ctggcgcgca tcatgctggc gattaaaagc gccctggccc acaaggatgc cacccccctg
     4141 ctggtatttg acgaaataga cgccaacgtg ggcggagaag tggcgcgggc tgtgggattc
     4201 aagatgcagc agctcggaaa caggcaccag gtgatctcca tcacgcactt cccgcaggtg
     4261 gcggccctgg cctcccatca ttacctggtt cagaaagcat ccgcgggcaa ccgcaccatc
     4321 tcctgcctgc gggaggtgag ccatgaagaa cgggtggatg aactggtccg catgctgggc
     4381 ggcggcggag accacgcacg cacgctggcc caggccctgc tgcagccctc ctgaacggga
     4441 aacgccggtg ggggagaggc cgccctattt ccccaggctc cccaggcgtg gggtgaaacc
     4501 ccgcagcctc agggaattga atacgacgca cagggaactc aaactcatcg ccccggcggc
     4561 aatcatggga ttcagcgtaa ggccgaaggc gggatataaa cacccggcgg ccaacggaat
     4621 gccgatgatg ttgtaaaaga aagcccagaa aagattctgc ctgatgatga gcagcgtggt
     4681 gcggcccagc tggacagctt ccgccacgcc tgcggggttg cttttcatca gcacgatgtc
     4741 ggaggattcc atcgccagct ccgttccgga tttcatggaa atgcccaccc gggcgcaggc
     4801 cagagccggg gcgtcgttca cgccgtcccc taccatcgcc accttgtccc cgcgttcctc
     4861 catcttcatc acatgggagg ccttttcatc cggaagcacg tcggagacca cctcgtcaat
     4921 gtggagttct ccggccatgt gccgcgccgt ggcggcgttg tcccccgtca gcatgacgac
     4981 gcgcaggttc atccgcttca aactgtccac cgccgcacgg ctgtccggtt tcagggaatc
     5041 cgccaccatg atgacgccgg cagggcatcc gcccctgccc acatacagcg gggacgcgcc
     5101 ctgccgcatg aactcccgca ggcggctttc atcctccttc caggaaattc c
//
LOCUS       DAOMSU010000153         5071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1495_length_5071_cov_2.364234, whole genome shotgun sequence.
ACCESSION   DAOMSU010000153 DAOMSU010000000
VERSION     DAOMSU010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1495_length_5071_cov_2.364234"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PK077_07175"
     CDS             <1..680
                     /locus_tag="PK077_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HRN24121.1"
                     /translation="DKRSGGDNRPMVHDHFVKLAPLWQLELYFKIAGKGNPDFYPDIF
                     YKAIKMDTRGKKDGELQLAFMKNACDAARQDLTDFFRKTGMLKPIDQELDDYTCARMT
                     ITEADCKNLIAYARKYKKPESPVIYYISVNSAEAYKNRLPVRGVYNQGVTEQGNRRII
                     SHDVWKNAVVFETYKDREMVRITMAGTDSRDNSSTTVPYPEGSTRIEAVSWDGRRTLV
                     YGRRPDK"
     gene            881..2065
                     /locus_tag="PK077_07180"
     CDS             881..2065
                     /locus_tag="PK077_07180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN24122.1"
                     /translation="MDKAGTSGGIRGKMLLFAAFLIVCFNLRTGFDSPDPLLGTIERD
                     MGLSLENSGLFALLPVFVLGVAAPISPRVARRMTPWKIIFWFQLLAVAGIFWRSWDGV
                     AGLYGGMVLMGLGMGIAGAAIPGLIKHQFPDRASAMMGVYSAMIGVGSAAASGLSVPL
                     SNMLGGWRFGLGIWIIPILLGMLVWGAYFLRHPDGVFQNDSAASGRSLLRSGKAWQVT
                     VFYLSRVGAAYFFYTWIPIFLRQRGMSYEDAGFILSVAMFAQLPATLSAHVLEKAMGG
                     RGLLIVMAMALAALSCWGILYFPLDWAVWMAILFGLATGTVFSRGMALMVERARTPSE
                     SIRLSGMSQGIGFTMGALLSLLFTSFLHQGGSFLPFCLVYTLFCVLGMVSGRMSARPG
                     YV"
     gene            2232..3257
                     /gene="pheS"
                     /locus_tag="PK077_07185"
     CDS             2232..3257
                     /gene="pheS"
                     /locus_tag="PK077_07185"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRN24123.1"
                     /translation="MKEEIVRIQRDALARIAQASDRRGVEDARVAILGKKGELTMAQT
                     GMKDVPREEKPAVGQLLNEARKAITEALDAKLEEVQAQADKAAVAGVDLTLPARSLPS
                     GGLHPLTIVRDEAVRILRHMGFALADGPEIEDEFHCFDALNTPEDHPARNEKDTFYFD
                     SGKLLRTHTSSVQIRSMEKQLPPVRVIAPGSAYRRDEIDATHLSAFNQLEGLYVDTDV
                     SVGDLKGTLEYFLRALFGSGTEVRFRPHFFPFTEPSFEIDVKLKVDGQAPRWVEIAGC
                     GMVDPNVFEAVDRELGLDPRSQARYTGLTGFAFGIGLDRLAMIRWGIRDIRALIENDV
                     RFLAQFQ"
     gene            3278..>5071
                     /gene="pheT"
                     /locus_tag="PK077_07190"
     CDS             3278..>5071
                     /gene="pheT"
                     /locus_tag="PK077_07190"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRN24124.1"
                     /translation="MKISLNWLSQYIDLSGLSVDEMSDMLTFAGIEVEDIRQQGVDSP
                     YVVVARVAAAEQHPQADRLKVCQVDVGDGTLHQIVCGAQNYKVGDKVPCALPGAVLPG
                     NFEIKVGKLRGVESRGMLCSASELGLPDKEHGLWILPQELEPGTPISQVVKADTLVEV
                     EVTPNRPDLLSHNGMAYELAAISGREYRPVSIDDAGVELEPAGDFVRLDQPELNPYYT
                     AVKISGVNVQESPEWLKERLVAVGLRPINNIVDITNFVLHELGTPLHAFDAAKVQGGI
                     VTRTAYEGETIKALDGQEYTLNCTDLVVADQSGKALAIGGVMGGEESGVTDATTDIIL
                     ESAWFKPSSVRATSRRLALSSDSSYRFERGTSAWNVLRGSVRAVELILQLAGGTASPT
                     YVAGSPVPNPAHASMPSCGGADGPVSVPASLKQGKGATVTNELGFVKLPWKSLDQISG
                     GSISHEEGARVLTALGLKQVPDSPECWLIPPHRLDLTRPCDLLEEIVRVFGLDGIPSR
                     FSGPFVAESPVDAAYNFQMELRRKLAALGFYETQTIKLIASESADGTIAQVKDALPLR
                     PLQEGDLIRVSLPLSEDHSVLRPAHTPGLIAA"
BASE COUNT          920 a         1499 c         1560 g         1092 t
ORIGIN      
        1 cggacaagcg ttccggcggg gataaccgtc cgatggtgca cgaccatttt gtcaagctgg
       61 ctcccctgtg gcagctggag ctgtatttca agatagccgg gaagggcaat cccgactttt
      121 atccggatat cttttataag gccatcaaga tggataccag gggcaagaag gatggagagc
      181 tccagcttgc gttcatgaag aacgcctgcg acgccgccag acaggacctg acggattttt
      241 tccggaaaac gggcatgctc aagcccattg accaggaact ggacgactac acatgcgccc
      301 gcatgaccat cacggaggcc gactgcaaga acctgattgc ctacgccagg aagtacaaaa
      361 agccggaaag ccccgtcatt tactatattt ccgtcaatag cgcggaagca tataagaacc
      421 gcctgcctgt ccgcggcgtc tataaccagg gcgttacgga gcagggcaat cgtcgcatta
      481 tttcccatga cgtgtggaaa aacgccgtcg tctttgaaac atacaaagac agggagatgg
      541 tccgtatcac catggcgggg acggattcca gggataattc ctccaccacg gtcccctatc
      601 cggaaggctc tacgcggatt gaggccgttt cctgggacgg caggcgtacg ctggtgtacg
      661 gcagacgccc tgacaaatga aagcttaaga ggttagcgtg ttcgggcgcc gccgttctcc
      721 gggacggcgg cgctttttca tgacaggggg ggcggatgga aaaccgcaag aggattgctc
      781 atcctgatgg ccttttctcc ataccgtatc ttccggactg ctgacagccg gaaggctgtt
      841 gcttttgaac aggggcgggg gagaggctgt ataacatggg atggataagg caggaacttc
      901 cggaggcatc aggggaaaga tgctgttgtt tgccgccttt ttaatcgtgt gctttaatct
      961 gcgtaccggg tttgattctc ccgatccgct gttgggaacg attgaaaggg atatggggct
     1021 ttccctggaa aattcagggc tgttcgccct gctgcccgtc tttgtgctgg gggtggcggc
     1081 tcccatttct ccgcgcgtgg cacgccggat gacgccgtgg aaaatcattt tctggttcca
     1141 gcttctggcg gtagccggaa ttttctggcg cagctgggac ggcgtggcgg ggttatacgg
     1201 cggcatggtt ctgatggggc tgggcatggg gattgccgga gcggccattc ccggactgat
     1261 caagcaccag tttcctgacc gtgcttccgc catgatgggc gtttacagcg ccatgatcgg
     1321 cgtgggcagt gctgcggctt ccggcctgtc cgttcccctt tccaatatgc tgggaggctg
     1381 gcgtttcggc ctggggatct ggattattcc catcctgctg gggatgctgg tatggggcgc
     1441 ttattttctc aggcatccgg acggcgtgtt tcaaaacgat tcggcggcat ccggacgcag
     1501 cctgctgcgc agcggcaagg catggcaggt taccgttttt tatttgagcc gcgttggggc
     1561 tgcctatttc ttttatacct ggattcccat ctttctgagg cagcggggca tgagctatga
     1621 ggacgccggt ttcatccttt ccgtggccat gttcgcccag ctgcccgcta cgctctccgc
     1681 tcacgtgctg gaaaaagcta tgggagggcg gggcctcctc atcgtgatgg ccatggcgtt
     1741 ggcagccctc tcctgctggg gcatcctgta ttttccgctg gactgggccg tctggatggc
     1801 gattctgttc ggcctggcga cgggaaccgt attcagccgc ggaatggcgc tgatggtgga
     1861 acgggcccgg acgccttccg aatcaatcag gctttccggc atgtcccagg gaatcggctt
     1921 taccatgggc gccctgttga gcctgctgtt tacctccttc ctgcatcagg gaggctcctt
     1981 cctgccgttc tgcctggttt ataccttgtt ctgcgtgctc ggcatggttt ccggccgcat
     2041 gtccgcccgg ccgggatacg tgtaattttc tggggagata ttcatgatta agtattagtg
     2101 attaggaagc ggggttcatt gttctttccc tcgtttccgt attccgccgt tttctcttga
     2161 gtgacgtccc tgcggcccga agggcttgac gctggcgggg gggacgggta agctcttgcc
     2221 cgtcaaattt tatgaaggaa gagattgtac gcatccaacg ggacgccctg gcacgcatcg
     2281 cccaggcgtc tgacaggcgc ggcgtagaag acgcgcgcgt ggccatcctc ggcaaaaaag
     2341 gggaattgac catggcccag acgggcatga aggacgttcc cagggaggaa aaacccgccg
     2401 tcggccagtt gcttaacgag gcccgcaagg ccattacgga agccctggac gccaagctgg
     2461 aggaagtgca ggcccaggcg gataaggccg ccgtggccgg ggtggatctg acgctgccgg
     2521 cccgctccct gccgtctggc gggctgcacc ccctcaccat cgtgcgggat gaagccgtcc
     2581 gcattctgcg ccacatgggc tttgccctgg cggacggccc ggagattgag gacgagttcc
     2641 actgctttga cgcgctgaac acgccggaag atcacccggc ccgcaatgag aaggatacat
     2701 tttactttga ttccggcaag cttctgcgta cgcacacgtc ttccgtgcag atccgctcca
     2761 tggaaaagca gctgccgccc gtgcgtgtca tcgctcccgg ttccgcctac cgccgcgacg
     2821 aaattgacgc cacgcacctt tccgccttca atcagcttga aggcctgtat gtggatacgg
     2881 acgtttccgt gggcgacctg aaaggaacgc tggaatattt tctgcgcgcc cttttcggct
     2941 ccggcacgga ggtgcgcttc cgcccccatt tcttcccgtt cacggaaccc agttttgaaa
     3001 ttgacgtcaa gctgaaggtg gacggccagg ccccccgctg ggtggagatt gccggctgcg
     3061 gcatggtgga tcccaatgtt tttgaagccg tggaccgtga actggggctg gacccccgct
     3121 cacaggcccg ctacacgggg ctgaccggct ttgccttcgg catcggcctg gaccgcctgg
     3181 cgatgatccg ctggggcatc agggacatac gcgccctgat tgagaacgat gtgcgcttcc
     3241 ttgcccaatt ccaataattt ttacctgttt tcccgttatg aagatttccc ttaactggct
     3301 ttcccagtat attgacctgt ccggcctgag cgtggatgaa atgtctgaca tgctgacctt
     3361 tgccggcatt gaggtggaag acatccgcca gcagggcgtg gattccccat acgtggtggt
     3421 ggcccgcgtg gccgccgcag aacagcatcc ccaggcggac cggctgaagg tctgccaggt
     3481 ggacgtgggc gacggcacgc tgcaccagat tgtgtgcggc gcgcagaact acaaagtggg
     3541 agacaaggtg ccctgcgccc tgccgggagc ggtgctgccc ggcaactttg aaatcaaggt
     3601 gggcaagctg cgcggcgtgg agtcccgcgg catgctctgt tccgcctccg agctggggct
     3661 tcccgacaag gagcacggtc tctggattct cccccaggaa ctggaacccg gcacgcctat
     3721 ttcccaggtt gtcaaggcgg ataccctggt ggaagtggaa gtgacgccca accggccgga
     3781 cctactttcc cacaacggca tggcgtatga actggccgct atctccggcc gggagtacag
     3841 gcccgtttcc attgacgacg ccggggtgga actggaaccc gccggagact tcgtgcggct
     3901 ggaccagcca gagctgaacc cgtactacac cgccgtaaaa atcagcggcg tgaacgtgca
     3961 ggaaagcccg gaatggctga aggaacgcct ggtagccgtc ggcctgcgcc ccatcaacaa
     4021 catcgtggat attaccaact tcgtcctgca tgaactggga acgcccctcc atgcctttga
     4081 cgcggcgaaa gtgcagggcg gcatcgttac ccgcaccgct tatgaagggg aaaccatcaa
     4141 ggccctggac ggccaggaat acacgcttaa ctgcacggac ctggttgtcg cggaccaatc
     4201 cggcaaggcg ctcgccatcg gcggcgtgat gggtggggag gaaagcggcg tgacggacgc
     4261 caccacggat atcattctgg agtccgcctg gttcaagccc tcctccgtgc gcgccacgtc
     4321 ccgcaggctg gccctgtcct ccgactcctc ctaccgcttt gaacgcggca cttccgcctg
     4381 gaatgtcctg cgcggttccg tgcgcgccgt ggaactgatt ctccagctgg cgggcggaac
     4441 ggcttccccg acgtatgtgg ccggttctcc cgttcctaat ccggcgcatg cttccatgcc
     4501 ttcctgcggg ggggcggacg gccccgtttc cgtgcccgct tccctgaagc aggggaaggg
     4561 cgccacggtg actaatgaac tgggctttgt aaaacttccc tggaagtcgc tggatcagat
     4621 ttccggcggt tccatttccc atgaggaagg ggcacgcgtc ctgacggctc tgggtctgaa
     4681 gcaggttccg gattcccctg aatgctggct gatccccccc caccgcctgg acctcacgcg
     4741 tccgtgcgac ctgctggagg aaatcgtgcg cgttttcggt ctggacggca taccgtcccg
     4801 cttctccggt cccttcgtgg cggaatcccc ggtggatgcg gcctacaatt tccagatgga
     4861 gctgcgccgc aagctggcgg ccctgggatt ttatgaaacc cagaccatca agctcatcgc
     4921 ctctgaatcc gcggacggca ctatcgccca ggtgaaggat gccttgcccc tgcgtcccct
     4981 tcaggaaggg gatctcatcc gcgtgtccct gccgctcagc gaggatcact ccgtgctgcg
     5041 tcccgcccat acgcccggcc tgattgccgc c
//
LOCUS       DAOMSU010000154         5054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1502_length_5054_cov_2.247249, whole genome shotgun sequence.
ACCESSION   DAOMSU010000154 DAOMSU010000000
VERSION     DAOMSU010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5054
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1502_length_5054_cov_2.247249"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /gene="trpD"
                     /locus_tag="PK077_07195"
     CDS             <1..997
                     /gene="trpD"
                     /locus_tag="PK077_07195"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HRN24125.1"
                     /translation="RISRILDALAAGQDLTADMAAAGFADIMDGRMTPAQAGCFLMGL
                     RMKGETPLEMAHAVGIALGRANRVEGLEGDCIDVVGTGGDGRNSFNCSTATALTLAGM
                     GYRVVKHGNRAVSSSCGSADALEGLGFPLDVAPEDVRRLLDERNFAFLFAPNFHPAFR
                     NVGPIRRELGIRTLFNLLGPLINPARPTHILLGVARPELVELLAETLRQSHIRKAAVV
                     YGAGGYDEVTPLGPTKMMIVHNGNLTPMSLDPSDYGIQPCNPEELAVHSKSEAVDVLK
                     NILAGKGPRAMMDMVILNVGVAMFLLEEHMDLSVCMAKAREAVCAGIGRRTLHAA"
     gene            987..1364
                     /locus_tag="PK077_07200"
     CDS             987..1364
                     /locus_tag="PK077_07200"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24126.1"
                     /translation="MLLDRFREAKAEEIALLMDMASRRELPRPWKGRRPDFLKAIAEP
                     PDGQPVAVIAEFKQSSPSRGVIASGLKPEEVAEQYAASGASCISVLTEEQFFGGRIGY
                     LERMSRAGLPLLRKDFIFHQLQV"
     gene            1397..1765
                     /locus_tag="PK077_07205"
     CDS             1397..1765
                     /locus_tag="PK077_07205"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24127.1"
                     /translation="MRLTPDARTLRVLREQAEAYGMHAVVEVFDPADLDIARESGARI
                     IQVNARDLDTLKTDRQACLDMAKFRREGEVWIAASAMSAGAHLREAAEAGFQAVLMGT
                     ALMDGGRPGEKLAAILEETI"
     gene            1762..2409
                     /locus_tag="PK077_07210"
     CDS             1762..2409
                     /locus_tag="PK077_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HRN24128.1"
                     /translation="MKKHSFAIKVCGITRQSDLSTAMGMGAHFCGFIFHPGSPRYIAP
                     PRAAALDSALIRRVGVFVNQNAEEIMDIMKTARLEFAQLHGTHSLDCARRIGPEKVIR
                     VLWPDRYESVDALQREMELWADSCAWFLLDAGTQGGGHGVSLDWTSLASLSSPRPWFL
                     AGGLSSASLNRALEQCSPDGIDLNSGVEAIPGQKSPQKLLAALKPLTPYTPYHIA"
     gene            2406..3578
                     /gene="trpB"
                     /locus_tag="PK077_07215"
     CDS             2406..3578
                     /gene="trpB"
                     /locus_tag="PK077_07215"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRN24129.1"
                     /translation="MKKEGYFGNFGGRFVPEALRPPLMELEEAMDSIMPSREYRDALQ
                     DLLVHYAGRETPLTFCPRLSEKLGFRLWLKREDLLHTGAHKINNALGQALLAKMMGKT
                     HLIAETGAGQHGVATATAAARLNMDCAIFMGAEDVERQSMNVMRMKLLGATVFPIESG
                     SRTLKDAINEALRYWISHQADTLYCFGTAAGPHPFPTLVRQLQSVIGREARAQMLERA
                     GRLPDVVVACVGGGSNAIGMLHPFVEDEGVCLVGVEAGGTGEPGCYNSAPINLGSPGV
                     LHGHVTMLLQDKDGQIQPSHSISAGLDYPGVGPEHAYLAETGRVHYGVICDASALNAF
                     QTLTREEGIIPALESSHAVAWVLDHAEELPQGGDVLVNLSGRGDKDLGIVRKYINL"
     gene            3593..4381
                     /gene="trpA"
                     /locus_tag="PK077_07220"
     CDS             3593..4381
                     /gene="trpA"
                     /locus_tag="PK077_07220"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HRN24130.1"
                     /translation="MNNMQKTPLQEAVERAHALGRRAVIPFITAGFPDKDSFWTHLSR
                     IDESGADIIEIGVPFSDPVADGPVIEQASRDALARGVSLKWILDGLKARKGGFSAELV
                     LMGYVNPFYQYGLEQLARDAEEAGVSGFIVPDMPLEESGMFREVFSPRGLTLVTLVAP
                     NTSVERMREYKPYTSGFVYVVSVLGTTGGKANLEQSVTETMRRARSVFDVPLALGFGL
                     QTPDQLETLPEDARPDAAVLGSALLRHIGEGKDAGEFLEKWTRG"
     gene            4865..>5054
                     /locus_tag="PK077_07225"
     CDS             4865..>5054
                     /locus_tag="PK077_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24131.1"
                     /translation="MTASQSYAADVAFDGTQVNVAAGGTASYDIGTVTASTTLNFAGA
                     GTATITNLNGTAPEAILTV"
BASE COUNT          992 a         1441 c         1539 g         1082 t
ORIGIN      
        1 gcgcatcagc cgcattctgg acgccctggc cgccggacag gacctgacgg cggatatggc
       61 cgccgcaggc tttgcggaca tcatggatgg ccgcatgact ccggcccagg cgggctgctt
      121 tctaatgggg ctccgcatga aaggggaaac cccgctggaa atggcccacg ccgtgggcat
      181 tgccctgggc cgcgccaacc gggtggaagg attggagggt gactgcattg acgtggtagg
      241 cacgggcggg gacggccgca actctttcaa ttgctccacg gccacggctc tgacgctggc
      301 cggaatggga tacagagtgg tgaagcacgg caaccgcgcc gtgtcctcct cctgcggttc
      361 tgcggatgct ctggaaggcc tggggttccc gctggacgta gctccggagg atgtccggcg
      421 cctgttggac gagcggaact tcgctttcct gttcgcgcct aattttcatc ctgccttccg
      481 taatgtaggc cccatccgcc gggagctggg catccgcacc ctgttcaatc tgctggggcc
      541 cctcatcaac ccggcgcgcc ccacgcacat cctgctgggt gtggcccgtc cggaactcgt
      601 tgagctgctg gcggagacgc tccgccagtc ccacatccgc aaggctgccg tagtatatgg
      661 tgccggggga tatgacgagg tgacgccgct ggggcccacc aagatgatga tcgtccacaa
      721 cggcaacttg acgcccatgt ccctggatcc ctcggattac ggcatccagc cctgcaatcc
      781 ggaggaactg gccgttcatt ccaagtcgga agccgtggat gtgctgaaga acatcctggc
      841 ggggaaaggc ccgcgtgcca tgatggatat ggtgattctg aatgtggggg tagccatgtt
      901 cctgctggag gagcacatgg acctgtccgt ctgcatggcg aaagcgcggg aagccgtctg
      961 cgccggcata ggaaggagaa cgcttcatgc tgcttgaccg gttccgggaa gccaaggcgg
     1021 aggaaatagc cctgcttatg gacatggcct cccgccggga actgccgcgt ccgtggaagg
     1081 ggcgccggcc ggatttcctg aaagccattg cggaaccgcc ggacggccag ccggtggcgg
     1141 tcattgcgga gttcaagcaa tcctctccgt cgcgcggtgt catcgcctcc gggctgaaac
     1201 cggaggaagt ggctgaacag tacgctgcgt cgggagcctc ctgcatctcc gtgttgacgg
     1261 aagaacagtt tttcggcggc cgcatcggct atttggaacg catgagccgc gccgggcttc
     1321 cgctcctccg caaggatttc atttttcatc agcttcaggt gtaggatcta cgccggcttc
     1381 cgcgttgctg ctcattgtgc gtctgacacc ggacgcacgg acgctccgcg ttctcaggga
     1441 gcaggcggag gcttacggca tgcacgccgt tgtggaagtg tttgatccgg cggacctgga
     1501 cattgcccgg gaatccggcg cgcgcatcat ccaggtaaat gcccgcgacc tggatacctt
     1561 gaagacggac aggcaggcct gcctggacat ggcgaaattc cgccgtgagg gggaggtgtg
     1621 gatagcggcc agcgccatga gcgccggggc gcatctcagg gaagccgcgg aagcggggtt
     1681 tcaggccgta ctgatgggaa cagccctgat ggacggagga aggccgggtg aaaaactggc
     1741 agcaatttta gaagaaacaa tatgaagaag cattcatttg caatcaaggt gtgcggaatt
     1801 acccgccaga gcgatttatc aaccgccatg gggatggggg cccatttctg cggcttcatc
     1861 tttcatcccg gaagtccgcg ttacattgcg ccgccgcgcg ccgcggctct ggattccgcc
     1921 ctgatccgcc gcgtgggagt tttcgtgaat cagaatgcgg aggaaatcat ggatatcatg
     1981 aaaacggccc ggctggaatt cgcccagctc catggcaccc attctctgga ctgcgcgcgc
     2041 cgcatcggcc cggaaaaggt aatccgcgta ttgtggccgg accggtatga atccgtggat
     2101 gccctgcaaa gggaaatgga actatgggcg gacagctgcg cctggtttct gctggatgcc
     2161 ggcacccagg gcggcggcca cggcgtttcc cttgattgga cctccctggc gtctctgagt
     2221 tccccccggc cctggtttct ggcgggcggc ctttcttccg cctccctgaa cagggcgctg
     2281 gaacaatgct ctccggacgg cattgacctg aactccggcg tggaagccat ccccggccag
     2341 aaaagcccgc agaagctgtt ggcggccctc aaacccctca ccccttacac tccttaccac
     2401 atcgcatgaa aaaagaagga tatttcggca atttcggcgg ccgcttcgta ccggaagcgc
     2461 tgcgtccccc cctcatggag ctggaggaag ccatggactc catcatgccg tcccgcgagt
     2521 accgggacgc gctccaggat ctgctggtcc attacgctgg gcgtgaaact cccctcacgt
     2581 tctgtccccg cctttcggaa aaactgggct tccgcctgtg gctgaaacgg gaggacctgc
     2641 tgcacaccgg agcccacaaa atcaataacg ccctcggcca ggcccttctg gccaaaatga
     2701 tggggaaaac gcatctgatt gcggaaaccg gggcggggca gcacggcgtg gctacggcca
     2761 cggcggcggc ccggcttaac atggactgcg ccatctttat gggcgcagag gatgtagaac
     2821 gccagtccat gaacgtcatg cgcatgaaac tgctgggagc gacggttttc ccgattgaga
     2881 gcggttcccg cacgttgaag gatgccatca atgaggcttt gcgctactgg atttcccatc
     2941 aggcggatac cctgtattgt ttcggtacgg cggctggccc ccatccgttc cccacattgg
     3001 tcaggcagct tcaatccgtc attgggcggg aggcccgcgc ccagatgctg gagcgcgcag
     3061 gcaggctgcc ggatgtggtg gtggcatgcg tgggcggcgg ctccaacgcc atcggcatgc
     3121 ttcacccctt tgtggaggat gaaggcgtct gtctggtggg ggtcgaggcg ggaggcaccg
     3181 gtgagcccgg ctgctataac tccgccccca tcaatctggg cagtcccggc gtgctgcacg
     3241 gccatgtgac gatgctttta caggataaag acgggcaaat acagccgtcc cattccattt
     3301 ccgccgggct ggactatccc ggggtaggcc cggaacacgc ctatctggcg gaaacgggcc
     3361 gcgttcatta cggggtgatt tgcgacgcct ccgctttaaa cgctttccag acgctcacca
     3421 gggaggaagg catcattcct gcgctggaat cctcccatgc cgtggcctgg gtgctggacc
     3481 atgcggagga actgccgcag gggggggacg ttctggtcaa tctctccggc cgcggagaca
     3541 aggacctggg aatcgtcagg aaatatatca atctttaagc cgcatcattt ccatgaacaa
     3601 catgcagaaa acaccgttac aggaagccgt ggaaagggcc catgcccttg gcagacgcgc
     3661 cgttattcct tttatcaccg ccggatttcc ggacaaggat tctttctgga cccacctgtc
     3721 ccgcattgac gaatccggcg cggatattat tgaaatagga gttcccttct cggaccccgt
     3781 ggcggacggc ccggttattg aacaggcatc ccgggatgcc ctggcccgcg gcgtcagcct
     3841 gaaatggatt ctggacggcc tgaaagcccg caagggcggt ttttcagccg aactggtgct
     3901 gatgggatac gtaaatccct tttaccagta tggcctggaa cagcttgccc gggatgcgga
     3961 ggaggctggc gtcagcggtt tcatcgtacc ggatatgccg ctggaggaat ccggaatgtt
     4021 ccgggaagtt ttcagccccc gtggcctgac gctcgtcact ctggtggctc ccaatacttc
     4081 cgtggaacgc atgcgggaat acaagcctta tacttccggc ttcgtttatg tggtttccgt
     4141 tctgggaacc accggaggga aggctaacct ggaacagagc gtgacggaaa ccatgcgccg
     4201 tgcccggtcc gtttttgatg tgccgctggc gctgggcttc gggcttcaga cgccggacca
     4261 gctggagacg ctgcctgaag atgcccggcc ggatgccgcc gtgcttggga gcgctctgct
     4321 caggcacatt ggagaaggga aggacgccgg ggaatttttg gaaaaatgga ccagaggttg
     4381 aactgtacgt ttctccttaa cctttaagga tgggcctgca taacttattg tgcaggccca
     4441 tcttatttta tctggtaata gttcgtgaaa atacggattc gcaatccgct ttacttttat
     4501 tcacatatta ttcaattttt taaagaaaag ctgtattgac agttaatgcg ttatatattt
     4561 tcgacataat ctgttggagg gaagccggga tttttttgac ggaaagaacc tcccggatat
     4621 tccggaaaac cattgtaact gattcttttc ctttgacagc ggattgcgaa ttccttattt
     4681 atattcacaa tcatatttcc cttgctgtgt tgtggggaag gttcttattt gttctattaa
     4741 atacctaatt atcaaacatt acatgaaatt acacctccct ttgaatttgt tggctgccct
     4801 gatgacctcc ttttccggag taacgcttgg caccgccacg ttggcaggag tttctggtct
     4861 tctgatgaca gcatcacagt cttatgcggc ggatgttgcc tttgacggaa cgcaggtgaa
     4921 tgtggcggcg ggaggtacgg cctcctatga cattggtact gtcacggctt ctaccacctt
     4981 gaattttgcc ggagccggca cggccaccat caccaatttg aacggaacgg ccccggaagc
     5041 catactgacg gtaa
//
LOCUS       DAOMSU010000155         5046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1505_length_5046_cov_2.280505, whole genome shotgun sequence.
ACCESSION   DAOMSU010000155 DAOMSU010000000
VERSION     DAOMSU010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1505_length_5046_cov_2.280505"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>412
                     /locus_tag="PK077_07230"
     CDS             <1..>412
                     /locus_tag="PK077_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24132.1"
                     /translation="REVSGKGASAGDWERIVLARTVDFPEGYEIPSDVNRNAVPWPDI
                     LADYSLMSTPGKGWSAAWQSYSGEKHYPIKPVDHPYDASKIFEQWVAGCICLALAMVA
                     LFFLVRTRGRKMSLNGKEVTAAGVVFRVDDIARLD"
     assembly_gap    413..422
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <423..654
                     /locus_tag="PK077_07235"
     CDS             <423..654
                     /locus_tag="PK077_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24133.1"
                     /translation="KLKGLAIATLKPECGGARIRLDGLTYGGFRKEDSPHNAEDFMKA
                     LLSLYRGEIVDYEEIPDAGGPPARQEDEERAV"
     gene            651..1862
                     /locus_tag="PK077_07240"
     CDS             651..1862
                     /locus_tag="PK077_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24134.1"
                     /translation="MSESLPPEKGANDRAFGVACWTLGGLAFVLLLMVGVSFSLSVRT
                     VVVEKVVEKPVPVPAREKQERPAGVVENGAAEETVHMADVPAPPSEKPRSVEEMLREA
                     DLSDPAADSVPVAAAVAGGEDSASGASLPAEDPDEVMPPMTEEVAELVKAARYAQIEG
                     DLKVAVVKLEQAMRLEPDNPVVLYYYGLTYEWLRNADKSREFFLKVYTQREKAGKYFA
                     RAARHLQAGFSSPADLRGDMSFGTILEYRDPECPAGERVKLMVPILMKDGLNVRPEDL
                     RVIVQFFDKVNGKKVEKTHAPEPSSRCVTEPADWADGEEIMEITYYMPPLTEEETIAY
                     GSLKYYGYTAKLYYKGEPMDCHASPPVLFLLEQMNQSSPSGLPEIYDGGLLPPVEAAP
                     VSESYESLLPP"
     gene            1878..3401
                     /locus_tag="PK077_07245"
     CDS             1878..3401
                     /locus_tag="PK077_07245"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRN24135.1"
                     /translation="MFDLPHNFGDYTLVAFIGRTRGGILYQAIQQGMDRSVFLELLDP
                     DNPEGVGVEDFLMKARTRAAINAPVLGTVYEASQAQGYWFVTSEQLGGSSLQSMLDRG
                     QTLSMKDLLKVIETVGNVCGRYERLRTAFNIMEPRHIFLDDKSAVRLMNTAVPGDFHE
                     ETSRDQMKRLGIDLPPLVTPDVPGTTRMRTLLEWMREGQNGKPMQWDQVMELVAAVRE
                     QLGLSPRVTTHRYTVPVESRGKAGKRLLWAGTGLLGAGIAAAAVLLLFPREKETVSRP
                     HVPAPKHYPDFSASDHTEVRVTLPGGGELIVGAHEITLESYRLFLDQWARLTPELREE
                     YSHPDQPDKRTASHIPQDWEAMWKAASTPGGKWKGRKITPRSPVVNVTFWDAWAYASW
                     KPVAPGEPRYRLPERGEWMALGNMLETGEKGDRTLVIDRYSNDHDLKTGVCGMASGVM
                     EWTSSMEKDPARVKEPPGPVACGGDWRQPGISNRVEYLRSRGERRDNLGFRIVRNAR"
     gene            3436..4278
                     /locus_tag="PK077_07250"
     CDS             3436..4278
                     /locus_tag="PK077_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24136.1"
                     /translation="MKHILAMLLALLGASGALAQIYVKVVPEKKTFLSGEAMYMRLTI
                     TNNSGVPVELKSREYSSWLDIHVEHSTAGAELPQSKFALFPPLKVPAGMSVSRKIDLR
                     HFYDLSREGNYHVQAVVKMPNQKDMFASQKSLFAVRTGTPMWSQTVAIPASAKRCTFS
                     VCTIAVRGIQKLYVQTKDPDTGVAYNAVCIGDWLGTTRPQCMVDGKANVHVFFPTTPM
                     LSAYSRINYRGTMEKLQYYKKVVGAPSMVFLPDGSVRVTGAVHYDPTVKPKTVPDAAD
                     VPGM"
     gene            complement(4381..>5046)
                     /locus_tag="PK077_07255"
     CDS             complement(4381..>5046)
                     /locus_tag="PK077_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HRN24137.1"
                     /translation="EAAGLHAPGPVSNDAAMGTLVVSNGSTTRTSRRPQLGTLSAEQR
                     PIPNGDTAYLGVVFGTPCTIQEVTKDGPAAQAGAKAGDEILAVDGTPVTALDAVSPIL
                     SRKKVGERVTLEVKRKDKRLSYTITLGSRRQALGGNAPEDSNDLISGGFSKRRDDFPM
                     VLQHDTPSRYTLMGGPLLNLKGELIGMNIARVNRAENYALPISVVQKSVQNILKNAPQ
                     PEK"
BASE COUNT         1017 a         1349 c         1578 g         1092 t
ORIGIN      
        1 ccgtgaggtg agcgggaaag gggcgtccgc gggggactgg gagcgcattg ttctggcgcg
       61 caccgtggat tttccggagg gctacgaaat tccttccgac gtgaatcgga acgcggtgcc
      121 gtggcctgac attttggcgg attacagtct gatgtccacc cccggcaagg gctggagcgc
      181 tgcgtggcaa tcttattccg gggaaaagca ctatcccatc aagccggtgg accacccgta
      241 tgacgcctcc aaaatttttg aacagtgggt ggccgggtgc atttgcctgg cgcttgccat
      301 ggtggctctg tttttcctgg tgagaacgcg cggcaggaaa atgtccctga acgggaaaga
      361 ggtgacggcc gccggcgtgg ttttccgcgt ggatgacatt gcccgtctgg atnnnnnnnn
      421 nngaaactga agggactggc catagcgacg ctgaaaccgg aatgcggagg agcccgaatc
      481 aggctggacg gcctgactta cggcggcttc cggaaggagg attctcccca taacgcggaa
      541 gatttcatga aggctcttct gtctctctac agaggagaaa tagtggatta tgaagaaatt
      601 cctgatgccg gcgggccgcc tgcccggcag gaggacgagg aacgtgccgt atgagcgaat
      661 cccttccacc ggaaaagggc gccaatgacc gtgcgttcgg cgtcgcctgc tggacgctgg
      721 gtgggctggc gtttgttctg ttgttgatgg tgggcgtttc tttcagcctg tctgtccgta
      781 ccgtggtggt ggaaaaagtg gtggagaagc ctgttcccgt tccggcacgg gagaagcagg
      841 agcgtccggc cggagtcgtt gagaatgggg cggctgagga gacagtgcat atggcggatg
      901 ttccggcacc cccttctgaa aagccccgtt ccgtggaaga aatgctccgg gaagccgatt
      961 tgtcagatcc ggctgccgat tctgtaccgg tggccgcggc cgttgcggga ggggaggact
     1021 ccgctagcgg ggcatccctt cctgctgagg atccggacga ggtcatgcct cccatgacgg
     1081 aggaagtggc agagcttgtc aaggcggctc gttacgccca gattgaaggt gacctcaaag
     1141 tagcggtcgt gaagctggag caggccatga ggctggagcc ggataatccg gtggtgcttt
     1201 attattatgg acttacctat gaatggctga ggaatgcgga taagtcacgg gagttttttc
     1261 tgaaagtata tacccagcgg gagaaggcgg ggaaatattt tgcgcgtgcg gcgcggcacc
     1321 tccaggccgg cttttccagc ccggcggatt tgcgggggga tatgtccttc ggcactattc
     1381 tggaataccg ggatccggaa tgccccgccg gggagcgcgt caagctgatg gtgcctattc
     1441 tgatgaagga tggcctgaat gtcaggccgg aggatttgcg cgtcattgtc cagttttttg
     1501 acaaagtcaa tgggaagaaa gtggaaaaga cgcacgcgcc cgagccttct tcccggtgcg
     1561 ttacggaacc cgcggattgg gcggacggtg aggaaatcat ggaaataacc tactacatgc
     1621 ctcccctgac ggaagaagag acgattgctt atggaagcct gaaatattac ggatataccg
     1681 ctaaattgta ttacaaggga gaaccgatgg attgccatgc ttcccctccc gtgctatttt
     1741 tactggaaca gatgaaccag agttcccctt ccggactgcc cgagatttat gacggcggtt
     1801 tgcttccccc tgtagaggcg gctccggttt ctgagtctta tgaatccctt ttgcctccct
     1861 gatgcctgat ttgtccaatg tttgatcttc cccacaactt tggcgattac acgctggtgg
     1921 cattcattgg ccgcacgcgc ggaggaattc tttatcaggc catccagcag ggcatggacc
     1981 gttccgtgtt tctggagttg ctggatccgg acaatccgga gggggtgggc gtggaggatt
     2041 ttctgatgaa ggcccgcacg cgcgccgcca ttaatgctcc ggtgctgggc accgtctatg
     2101 aagcgtccca ggcccaggga tactggttcg tcaccagcga acagctggga ggctcatccc
     2161 tccagtccat gctggaccgc gggcagaccc tgtccatgaa ggatttgctg aaagtgattg
     2221 aaacggtcgg caacgtgtgc ggcaggtatg agcgtctgcg gaccgctttc aatatcatgg
     2281 agccgcgtca cattttcctg gatgacaagt ctgccgtgcg gctgatgaat acggccgtgc
     2341 ccggtgactt tcacgaagaa acatcccgcg accagatgaa gcgcctgggc atcgacctgc
     2401 ctcctctggt gactccggat gtgcccggaa ccacgcggat gcgcaccctg ctggaatgga
     2461 tgagggaagg gcagaacgga aagccgatgc aatgggacca ggttatggag ctggttgctg
     2521 cggtccgcga acagctgggg ctttcccctc gggttaccac ccaccgctat accgtccccg
     2581 tggaatcccg cggaaaagcc ggaaaaaggc tgctttgggc cggaacgggc ctgctgggcg
     2641 cggggatagc cgcggctgcc gttcttcttt tattcccccg ggagaaggaa accgtatccc
     2701 gtccccatgt tccggctcct aaacattatc cggatttttc cgccagcgat catacggaag
     2761 tccgcgtaac tcttcccgga ggaggggaac tgattgtggg agcccatgaa attaccctgg
     2821 aatcctaccg cctgtttttg gaccagtggg cacgcctcac tccggagctg agggaggaat
     2881 attcccaccc ggaccagccg gacaagagga ctgcttccca tattccccag gactgggagg
     2941 ctatgtggaa ggcggcttcc acgccgggag gcaaatggaa aggcaggaaa ataacacccc
     3001 gctctcccgt cgtcaacgtc actttctggg atgcctgggc gtatgcgagc tggaagcccg
     3061 tggcccccgg agaaccgcgc taccgcctgc ccgagcgcgg ggagtggatg gctctgggga
     3121 atatgctgga aacgggtgag aagggggaca ggacgctggt gattgacaga tacagcaatg
     3181 atcatgattt aaagaccggc gtgtgcggca tggcttccgg cgtgatggaa tggacttctt
     3241 ccatggaaaa ggatcctgcg cgcgtgaagg agcctccggg accggtggcc tgcggcggag
     3301 actggaggca gcccggcatc tccaaccggg tggaatacct gcgctcccgc ggcgaaagac
     3361 gggacaacct gggattcaga atcgtgcgga atgcccgctg acccgtttaa cgacaatact
     3421 taacatacca ataccatgaa acatatcctt gcaatgctgc tggcccttct gggggcgtcc
     3481 ggggccctgg cccagattta cgtaaaagta gttccggaaa agaagacttt cctcagcggg
     3541 gaagccatgt acatgaggct gaccattacc aacaattccg gcgtgccggt ggaattgaag
     3601 agccgggaat attcctcctg gctggatatc catgtggagc attccactgc gggggcggaa
     3661 ctcccccagt ccaagtttgc cctgtttccg cctctcaagg ttcctgccgg catgagcgtt
     3721 tcccgcaaga ttgacctgcg tcatttctat gacttgtccc gtgagggcaa ctaccatgtg
     3781 caggccgttg tcaaaatgcc caaccagaag gacatgtttg cttcccagaa atccctgttt
     3841 gccgtccgga cgggaacacc catgtggtcc cagaccgtag ctattccggc cagtgccaag
     3901 cggtgcactt tttccgtttg caccattgcc gtgcggggga tccagaagtt gtatgtgcag
     3961 acgaaggatc cggatacggg agtggcctac aatgccgtgt gcatcggtga ctggctgggg
     4021 accacgcgtc cgcagtgcat ggtggacggc aaggcgaatg tccatgtttt cttccccact
     4081 acccccatgc tgagcgcgta ttcccgcatc aactaccggg gaaccatgga aaagctccag
     4141 tactataaaa aggtggtggg agctccctcc atggtctttc ttccggacgg aagcgtacgt
     4201 gtgacgggag ccgtccatta tgatcctacc gtgaaaccca agacggtgcc ggatgccgct
     4261 gatgttcccg gcatgtaaat ccgtcctgtt tcgccggatg tgccacgcag gggaaaagga
     4321 aaggcaaacg tacagcttct tccgtaccgg gaaaataaaa tgttccggta ttttcctgag
     4381 ctatttttcc ggctggggcg cgtttttcag gatgttttgc acgcttttct gcacaacgct
     4441 gatgggcagg gcgtaatttt ccgcccggtt cactctggcg atgttcatgc cgatgagttc
     4501 ccccttgaga ttgagcagcg ggccacccat cagcgtgtac cgggaagggg tgtcatgctg
     4561 gaggaccata gggaaatcat ccctgcgttt gctgaacccg ccgctgatca ggtcattgga
     4621 atcctcagga gcgtttcctc ccagggcctg gcgtctggaa cccagcgtga tggtgtagga
     4681 taacctttta tccttgcgtt tgacttccag tgtcactctt tctccaactt ttttcctgga
     4741 cagaatgggg gaaacggcgt ccagagcagt aacgggcgtg ccgtccaccg ccagaatctc
     4801 atctccggcc ttggctccgg cttgggcggc ggggccgtcc ttggtaactt cctgaatggt
     4861 acagggggtg ccgaaaacga cgcccaggta ggcggtgtct ccgttcggga tggggcgctg
     4921 ctccgcacta agggttccca gctggggccg tctggacgta cgggtagtgg agccgttgct
     4981 gactaccagg gttcccatgg cggcatcatt gctgacgggg ccgggagcgt gcagcccggc
     5041 ggcttc
//
LOCUS       DAOMSU010000156         5033 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1515_length_5033_cov_2.204299, whole genome shotgun sequence.
ACCESSION   DAOMSU010000156 DAOMSU010000000
VERSION     DAOMSU010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5033)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5033
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1515_length_5033_cov_2.204299"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2208)
                     /locus_tag="PK077_07260"
     CDS             complement(<1..2208)
                     /locus_tag="PK077_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HRN24138.1"
                     /translation="MVFSSLPIEEIRGELMEALRVPSPRILLKAPTGSGKSTGVPPMM
                     DDAGLGDRGLIVVVQPRRMAARLLARHVARLRGVELGKEVGYAVRFERYISSRTRIAY
                     VTDGMLERWLTEWPSLEGVSAVVFDEFHERSLSGDLSLGRVLDLQEGPRRDLAVVVMS
                     ATLEISGLREYMGGSCRVLEAQGRQYPVEVVYRAPRLVSDGRGRVAPPPIWEQAADVV
                     REAVREDDCGDVLVFMPGVYEIRKTVELLAGKPWMSGRDVFPLYGSLAPEQQNCAVER
                     GDIPRVIVSTNVAETSLTIEGVRTVVDSGLVRRSGWDPYRGMDTLHLVKISKASAAQR
                     AGRAGRVAPGRCFRLWSEAEQARKEDFDPPECFRVDLAGAVLNLAAWGVTVPENFRWL
                     DVPAPLVMQRAVNLLAALGATEEDGSLTDTGKRMTAFPLPPRLARLMVAGQDEQCAVE
                     LAAVAALMQGEGVAVKGGLNDHLRDSADYTDFQAEWRAVEKAVDAGFGAAACTRWGIS
                     SRGAREAWMAYRQLLSVGSRGKARETPGPDFTAARPAVVRAMIESFADHVGVRNGVAA
                     NTCRMAGGVGGRLAEGSVVFQGEHFVAAEVAELSGKAVETRVGRCTLIAPEDLRSIWP
                     ERFSCGEEAVFDAALRRVRLHRKLMYGDLVLEDRDRGDAPTELAAPVLAEKVVDGTLK
                     LVKWNDAVEQWIRRLNGLSVWMPELELPRFSEEDKLVAISLVCEGAVGYKDIKE"
     gene            complement(2245..3156)
                     /locus_tag="PK077_07265"
     CDS             complement(2245..3156)
                     /locus_tag="PK077_07265"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRN24139.1"
                     /translation="MLQRSFSTVVLLGLLAGALWWDCELGYYGLVCIFCILAAWEWRH
                     MLLRSGKASQPNLSFLFGVAYPVLLSWSCCITKFRESALAEQVSFLIPLPLLVALAAP
                     VLLVVVAFIREMKHPVDGSRALRSVGTTLLSFIYPVWLFSFAILALHLAYMRAGCVYP
                     AIVMCVLWVVLVTKMADIFAYVSGFLLGGRLFSRRLIPHISPKKTWEGLLGSWVLTNA
                     AAIGLVFPMFGISVLSITQVLVLMGCTSLIFLLSVYGDLAGSLVKRSLAIKDSGSLLP
                     GIGGIFDLIDSPSFTVPFFWFLLACIG"
     gene            complement(3210..3740)
                     /locus_tag="PK077_07270"
     CDS             complement(3210..3740)
                     /locus_tag="PK077_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24140.1"
                     /translation="MFITEKGAFFSIWGKSWRRDVSVTDLQVIVYLIKRPHDAILLSL
                     FPEGDKSKWKPVHPEISGGGRGRKEENSWVDKSTIKFEILCGSPAFVILELHGNGFHF
                     TDVFSAFLPLFVVACFLWFSVAASFPEREKWRDDCFPVCSGEGEDGENGRFPKRRRRK
                     GSISGLRLQRFSVHFA"
     gene            3739..>5033
                     /locus_tag="PK077_07275"
     CDS             3739..>5033
                     /locus_tag="PK077_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRN24141.1"
                     /translation="MHSFRWIRLTAFSALAAAAITSCASAATDFNQVGKQMSLLLQNF
                     HFSRKEFSDELSTKFLETYLRKVDPNKIFFTQQDVDALKRKYGKELDDYLMSGQMMDA
                     AQAMHALYRQRAMQRISYARDLLKKGGFTFDKDKSIERSRRKTAAWPKDEAEMQQVWK
                     DMVEEQLLSEILRRETVARLAKEQNKPDPLANEKPAEEKLLMRYERIQRNIQETDLED
                     VAETLLSAVALTYDPHTDYMGARQVDRFKISMGTELTGIGALLGSEDDGSTKITGIVV
                     GGPADKSGELKLNDRIVAIDSDNSGEMVDILFMKLDKVVDMIRGAENTQMRLKVEPAD
                     APGQAKIITLTRSKVPLKDELAKGEIIELTGAPEGRNRIGVLSLPSFYADMEGGDRRC
                     AKDVKKILERMNKENVDGLVIDLRSNGGGSLEEVRLMTG"
BASE COUNT         1278 a         1636 c         1247 g          872 t
ORIGIN      
        1 ttccttgatg tccttataac ccacggcccc ttcacagacc aaagaaatgg cgaccagctt
       61 gtcctcctcc gaaaaccggg gcagctccag ttccggcatc caaacgctca aaccattaag
      121 acggcgtatc cactgttcca cggcatcatt ccatttcacc agcttcaggg ttccgtccac
      181 tactttttca gccaaaaccg gggcggccag ctccgtaggg gcatccccgc ggtcacgatc
      241 ctccagcacc agatctccat acatcagctt ccggtgcaga cgcacgcgcc gcaaagcggc
      301 atcgaagacg gcttcctccc cgcaggaaaa acgctccggc caaatggaac gcaaatcttc
      361 cggcgcaata agcgtacagc gtcccacacg ggtttccacc gcctttccgg acaattccgc
      421 cacctctgcg gctacgaaat gctctccttg aaacaccacg cttccttccg ccagcctgcc
      481 gcccaccccg ccggccatgc ggcatgtatt ggctgccacg ccatttcgca cacccacatg
      541 gtccgcaaaa ctctcaatca ttgcacggac tacggcaggg cgcgccgccg taaaatccgg
      601 tcccggcgtt tcccgggctt ttccccggct gccgacggac agaagctgcc ggtatgccat
      661 ccatgcctcc cgcgccccgc gggaagagat gccccagcgg gtacacgccg ccgccccaaa
      721 accggcatcc acagccttct ccaccgcgcg ccactccgcc tggaaatccg tgtaatccgc
      781 agaatcgcgc aaatgatcgt tcagcccccc ctttaccgca acaccttctc cctgcatcag
      841 ggctgcaacg gcggccagct ccacggcaca ctgttcatct tggccggcca ccatcagacg
      901 ggccagccgg ggcggcaggg gaaaagcggt catccttttt cccgtatccg tcaggcttcc
      961 atcctcttcc gtcgcaccca gggcagccaa caaattcacg gcccgctgca tcaccagggg
     1021 ggcaggaaca tccagccaac ggaaattttc cggtacggtg acgccccagg cagccaaatt
     1081 caaaacggct ccggccagat ccacccggaa acattcgggc gggtcaaaat cctccttccg
     1141 ggcctgttcc gcctcactcc acagccggaa acagcgcccc ggagccacgc gcccggcacg
     1201 gcccgcgcgc tgagcggcgg acgccttgga aatcttcacc agatgcagcg tatccatccc
     1261 ccggtacgga tcccatccgg accggcggac cagcccggaa tcaaccaccg tacgcactcc
     1321 ctcaatcgtc agggaagttt ccgccacatt agtggaaaca atgacacggg ggatatcccc
     1381 ccgctccacg gcgcaattct gctgctccgg cgccagagac ccgtaaaggg gaaaaacgtc
     1441 ccggccgctc atccacggct ttcctgccag caactccacc gttttccgta tctcatacac
     1501 gcccggcata aataccagca catccccgca gtcatcctcc ctcaccgcct cccgcacaac
     1561 atccgccgcc tgctcccata tggggggagg agccaccctg ccgcgtccgt cactgaccaa
     1621 gcggggagcc cggtaaacga cttccacggg atactgcctg ccttgcgctt ccagaacccg
     1681 gcaggaacct cccatatatt cccgcagccc tgatatctcc agtgtggcgg acatcacgac
     1741 cacggccaga tcccggcgcg gcccttcctg cagatccagc acacgcccca gggataaatc
     1801 cccggaaagg ctgcgttcat ggaactcatc aaacaccacg gcgctcaccc cttccaggga
     1861 gggccattcc gtcagccagc gctccaacat gccgtccgtc acgtacgcga tacgggtgcg
     1921 ggaggaaatg taccgttcaa aacgcaccgc ataccccact tcttttccca gctccacccc
     1981 gcgcagccgg gccacatgcc gcgccagcag gcgagcggcc atccgccggg gctgtacaac
     2041 gacaatcagg ccacggtctc ccagaccggc gtcatccatc atcggaggaa cgccggtgga
     2101 cttgccggag ccggtaggag ctttcaacaa aatgcgcgga gaaggaaccc gcagagcctc
     2161 catcaattct ccgcggattt cctcaatggg aagagaagaa aaaaccataa cgggttacag
     2221 ggaatccgtt cgtgccgggg acattcagcc gatgcaggcc agcagaaacc agaaaaacgg
     2281 cacggtaaag gaagggctgt caatcaaatc aaaaattcct cctatccccg gcagcaggga
     2341 accggaatcc ttgatggcca ggctgcgttt aaccagggac ccggccagat ccccgtaaac
     2401 ggaaagcagg aaaatgaggg atgtgcatcc catcagtacc aatacctggg ttatggacaa
     2461 gacggaaatt ccgaacatcg ggaaaaccag ccctatggcc gccgcattcg tcagcaccca
     2521 ggaaccgagg aggccctccc acgttttctt ggggctgatg tggggaatga gcctgcggga
     2581 aaaaaggcgg ccgcccagca ggaacccgct cacgtaggca aaaatgtccg ccatcttcgt
     2641 taccagaacg acccacagca cacacatgac aatggccgga taaacacacc ccgccctcat
     2701 atacgccaga tgcagggcca gaatggcaaa agagaacagc cagacgggat aaataaagga
     2761 aagcagcgta gtacccacgg aacggagagc cctgcttcca tccaccgggt gcttcatctc
     2821 acggatgaag gcaaccacta ccaggaggac cggagcggca agtgccacca gaagaggaag
     2881 ggggatgagg aaagaaacct gttccgcaag ggcgctttcc cggaatttag tgatgcagca
     2941 tgaccaggac agcaatacgg ggtacgccac tccgaaaaga aagctcaagt tcggctggga
     3001 ggcttttccg gaacgcagca gcatgtgccg ccattcccag gcggccaaaa tacagaaaat
     3061 gcagaccaga ccgtaatacc caagttcgca gtcccaccac aaagccccgg ccagcaaccc
     3121 aagcaaaacc acggtgctga atgaacgctg cagcaaaaca cccatcttgc ttccggaaaa
     3181 actcttgtcc cgattcggta tgctcatcat cacgcgaagt gtacagaaaa ccgttgcaac
     3241 ctcaatcctg aaatgcttcc tttcctgcgg cgtctcttcg gaaaacgtcc gttctcccca
     3301 tcctcaccct ccccgctgca aacaggaaag cagtcatccc gccacttttc cctctccggg
     3361 aaggatgcag caacggaaaa ccaaagaaaa cacgcaacaa caaaaagcgg aagaaatgcg
     3421 gaaaaaacat ccgtaaaatg aaatccattt ccatgcagct ccaatatgac aaatgccgga
     3481 cttccgcaca aaatttcaaa ctttatggtg gatttatcca cccatgaatt ctcttctttc
     3541 ctccccctgc ctccgccgga tatttccgga tgaaccggct tccatttact cttgtcccct
     3601 tccggaaaca aggagagcaa aatagcgtca tgtggacgtt ttataagata gacaatcacc
     3661 tgcaaatcag taacacttac atcccggcgc cagctttttc cccaaatgga aaaaaacgca
     3721 cccttttccg ttatgaacat gcactcattc cgttggatta gactcaccgc attctcggcc
     3781 ctggccgcag ccgccattac ttcctgcgcc tctgcggcta cggacttcaa ccaggtgggc
     3841 aagcaaatgt ccctgctgct ccagaatttc cacttctctc gcaaagaatt cagcgatgaa
     3901 ctatccacta aattcttgga aacctacctg cgcaaggtag accctaacaa aatattcttc
     3961 acccagcagg acgtagacgc cctcaaaaga aaatacggta aggagctgga cgactacctt
     4021 atgtccggcc agatgatgga tgcggcccag gccatgcacg ccctttaccg ccagcgcgcc
     4081 atgcagcgca tctcctatgc gcgggatttg ctgaaaaagg gaggcttcac ctttgacaaa
     4141 gacaagtcta tcgaacgttc ccgccgcaaa acagccgcgt ggcccaagga tgaggcggaa
     4201 atgcagcagg tctggaaaga catggtggag gaacagctcc tgtccgaaat cctgcgccgt
     4261 gaaaccgtag cgcgcctggc caaggaacag aacaagcccg atcccctggc caatgaaaaa
     4321 cccgcggagg aaaaactgct tatgcgttat gaacgcattc aacgcaatat tcaggaaacg
     4381 gatctggaag acgtagcgga aacactgctc agcgccgtag ccttgacgta tgacccgcat
     4441 acggattaca tgggtgcgcg ccaggtggac cgtttcaaaa tctccatggg tacggaactc
     4501 accggcatcg gcgccctgtt gggcagtgaa gacgacggtt ccaccaaaat taccggtatc
     4561 gttgtgggag gaccggctga caaatccgga gaattgaagc tgaatgaccg catcgttgcc
     4621 attgactccg acaactccgg agaaatggtg gatatcctgt tcatgaagct ggacaaagtg
     4681 gtggatatga tccgcggagc cgaaaatacc cagatgcgcc tgaaagtaga gccggcagac
     4741 gcccccggac aggccaaaat cattacgctg acccgctcca aggtacctct gaaggatgaa
     4801 cttgccaaag gtgaaatcat tgaacttacc ggagctccgg aaggcaggaa ccgcattggc
     4861 gtgctgagcc ttccctcctt ctacgcagac atggaaggcg gagaccgccg ctgtgccaag
     4921 gatgtcaaaa aaatcctgga acggatgaac aaggaaaatg tggatggcct ggtaattgac
     4981 ctgcgcagca acggcggcgg ttccctggag gaagtgcgcc tgatgacggg ctt
//
LOCUS       DAOMSU010000157         4975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1544_length_4975_cov_2.517276, whole genome shotgun sequence.
ACCESSION   DAOMSU010000157 DAOMSU010000000
VERSION     DAOMSU010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1544_length_4975_cov_2.517276"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..83)
                     /locus_tag="PK077_07280"
     tRNA            complement(1..83)
                     /locus_tag="PK077_07280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Leu,seq:aag)
     gene            complement(187..702)
                     /locus_tag="PK077_07285"
     CDS             complement(187..702)
                     /locus_tag="PK077_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24142.1"
                     /translation="MNKLLLSLLGAALLPALTQCNTLEKDIAGINARNAEIAREPRGN
                     YFVGRRYHVPATRFWGYLRQPGQTWRTAQLVIMDESACRTPDRLPEYTGNPRYAYDNN
                     YEYRVYGKYTGKYGYEPNSNLRLPIFKPTRFEVANTTPGWLFKPSEKYDTGAVTLRPA
                     IMPSTTSLPVR"
     gene            complement(699..1493)
                     /gene="tcdA"
                     /locus_tag="PK077_07290"
     CDS             complement(699..1493)
                     /gene="tcdA"
                     /locus_tag="PK077_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HRN24143.1"
                     /translation="MNYEARFGGIGRLYGNSGLSLLRSARIAVVGIGGVGSWAAEALA
                     RSGVGTIILMDMDDLCITNTNRQIHALASTIGQSKTETMATRIREINPEAEVIPISNF
                     YTASNAEKLLSAEPDVIIDAIDSLIPKAHLIASCYRSKQPLVTCGGAGGRINPARIEI
                     DDLSRTRGDPLLSSLRYRLKKDYALPLGEKARKLKIPCVFSQETPVYPTCDGTTSCTR
                     DPEFQGKMGCDAGFGSVTHITGTFGFFAASAAIQMLLNKKKTPSSS"
     gene            complement(1818..2621)
                     /locus_tag="PK077_07295"
     CDS             complement(1818..2621)
                     /locus_tag="PK077_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HRN24144.1"
                     /translation="MITILFIGDVVGEPGRTAVKHMLPELKREHGADFIIVNGENAAA
                     GRGITPRLAQELLHAGVDVITTGDHVWDQAELAPWMESEPRVLRPVNYPQGTPGHGSV
                     VVETPKGKIAVMQVQGRSFIQPPLENPFLISEAEAKRLREEKGVRVIFVDMHAETTSE
                     KISTGYNLDGLVSAVVGTHTHVQTADETILERGTAYLTDAGMCGPAVGVLGREREPIL
                     QRFRTSMPAKFPVANWPVRLCGAVVQIDEATGRALSIARISKIVEKPAS"
     gene            complement(2654..3124)
                     /locus_tag="PK077_07300"
     CDS             complement(2654..3124)
                     /locus_tag="PK077_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HRN24145.1"
                     /translation="MKNILVAIDFSNATDAVIHQIGKLACPNDSIIHLLHIVEPSICY
                     EVSGVLPDEVPVPVMDQTEENEVISIAKNHLKQTAEKLSRLTDATIIQAVEDEFEISE
                     AVINYAEKHHIDMIVVGKHNHGFLSTVFLGSVASSVMRKSPVPVLVVPVTREEQ"
     gene            complement(3203..4168)
                     /gene="trpS"
                     /locus_tag="PK077_07305"
     CDS             complement(3203..4168)
                     /gene="trpS"
                     /locus_tag="PK077_07305"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRN24146.1"
                     /translation="MRIITGLQPSGKLHVGNYFGAMEPAVRMQESGESFYFLADYHAM
                     STVHDANTLRENCSNLATDFLAVGLDPEKCVFFRQSAVPEVNELAWILSTVCPMGLLE
                     RCHSYKDKIAKGFSPSNALFTYPVLMAADILMYDSDLVPVGKDQKQHLEVTRDLAGKM
                     NEQFGEGTCKLPDALIAESTAIVPGLDGQKMSKSYNNTLPIFGEEKAVRKLIMRIPTD
                     STPVEDPKPTEGSVILQLYKLFASAQGYENMVRDFQNGGCGYGDFKKRLFDAYWEYFR
                     PARERRAELEANQDYVRRTLEEGARKARETASVVLDRVRRAVGLA"
     gene            complement(4359..>4975)
                     /locus_tag="PK077_07310"
     CDS             complement(4359..>4975)
                     /locus_tag="PK077_07310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24147.1"
                     /translation="EEAYRNLCANGTRNTLEAFSGTPVIFCSSTAVYGITDGRWITEE
                     HNVYPSSGKNGLLIQAEQTVLAAGGTVVRLGALYGPGRCVLVSQYVTKGKALPGAMNR
                     WLNYIHRDDAAAALHLLCTLREPPAGIYNLTDRTPMQMGEIYSYLSNLLGKPAPQPEP
                     LPAEARRGFSNQRISCSRLMALGWEPLYPSFADGVHNVLAALEE"
BASE COUNT          925 a         1318 c         1529 g         1203 t
ORIGIN      
        1 tgctcgggag gggactcgaa cccctacggt ttatcaccac tagatcctta gtctagcgcg
       61 tctaccaatt ccgccacctg agcatggagg ccttaaggcg gggagttttt agcgcagaga
      121 aatgaaaata tcaagctttt tcttcttttt tatctttccg ggcccgtttt tgcggacagg
      181 tttttcttac cggaccggga gggaggttgt cgagggcatg atagcagggc gcagagtaac
      241 ggctccggta tcatattttt ccgagggttt gaagagccag ccgggggtgg tgttggccac
      301 ctcaaacctg gtcggtttga agatgggcag cctcagattg gaattgggtt cgtaaccgta
      361 tttgcccgtg tatttgccgt ataccctgta ttcatagttg ttgtcgtagg cgtagcgcgg
      421 atttccggtg tattccggca ggcggtccgg ggtgcggcag gcgctttcat ccatgatgac
      481 gagttgggcc gttctccaag tctggcctgg ctggcgcagg tatccccaga agcgcgttgc
      541 cggtacatgg tagcggcggc cgacgaagta gtttcccctg ggttcgcggg ctatttccgc
      601 attgcgggcg ttgatgccgg caatgtcttt ttccagggtg ttgcactggg tcaaggcggg
      661 aaggagggcg gcccccagca gggaaagcag gagtttgttc atgatgacga tggtgttttt
      721 ttcttattta agagcatttg aatggcggcg gaagccgcaa agaagccaaa ggttcccgtg
      781 atatgggtga cggagccgaa tccggcgtca caacccattt tcccctggaa ttccggatca
      841 cgtgtgcagg aggttgttcc gtcgcaggta ggatatacgg gcgtttcttg ggagaaaacg
      901 caggggattt tcagtttccg ggctttttct ccaagcggaa gggcataatc ttttttcagc
      961 ctgtagcgta agctggagag caggggatcc cccctggtgc gggagaggtc gtctatttct
     1021 atccgggcgg gattgatgcg ccctcccgcg ccgccgcagg ttaccagcgg ttgcttgctg
     1081 cggtaacagg aggcgatgag gtgggccttg ggtatcaggg agtcgatggc gtcaatgatg
     1141 acgtccggtt ccgccgacag cagtttttcc gcgttggagg ccgtgtagaa attgctgatg
     1201 ggaatgactt ccgcttccgg gttgatttcc cggatgcgtg tggccatggt ttccgttttg
     1261 gattgcccga ttgtggacgc caacgcatgt atttgccggt tggtgttggt aatgcagagg
     1321 tcgtccatat ccatcagaat gatagtgcct acgcctgacc gagccagggc ttccgccgcc
     1381 caggaaccta cacccccaat gccgacgacg gctatgcggg cgcttctgag aagagatagt
     1441 ccgctgtttc cgtacaggcg gcctataccg ccgaatctgg cttcatagtt catgaccacg
     1501 tgtctgttcc agagtatggg agattaagga gaataagggc gcccgggttt ctgtccgttg
     1561 cttcatcctg aaaatatgga gacgcgggaa atccttggat tccggacgcc acagccagct
     1621 tcccgttatt ccgggaaggg ggggcgggac gccggcagct ttttccttcc gcaagtgtga
     1681 tggagggcgt ccgttgaaac aggtttttct tataatccgc atcctggccg cgcttcacgg
     1741 ggtttccgcc atgtccggaa catccgcaga gcaccgccac cgggcacggg aagaggaatg
     1801 ctgctgaagc atgcatatca ggaggccggt ttttccacga ttttgctgat gcgggcgatg
     1861 ctgagcgccc tgcctgtggc ttcatctatt tgcacgacgg ctccgcagag gcgcacgggc
     1921 cagttcgcta cggggaattt ggccggcatg gaggtgcgga aacgctggag aataggttcc
     1981 cgttcccgtc ccagcacccc gacggcgggg ccgcacatgc cggcgtccgt caggtaggcc
     2041 gtgccgcgct ccagaatggt ttcatccgcc gtctgcacat gggtatgtgt cccgacgacg
     2101 gcagaaacca ggccgtccag attgtagccg gtggagattt tttcactggt cgtttccgcg
     2161 tgcatgtcca caaagataac ccgcacgcct ttttcttccc tgagtctttt ggcttccgct
     2221 tccgagatga ggaaggggtt ttccagcggc ggctgaatga atgagcgccc ctgaacctgc
     2281 atgacggcta ttttcccctt gggagtttcc acgactacgc tcccgtgccc cggtgttccc
     2341 tgcggatagt tgacgggacg cagcacccgc ggttcggatt ccatccacgg ggccagctcc
     2401 gcctggtccc agacatggtc tcccgttgtg atgacatcca ctccggcgtg cagcagttcc
     2461 tgtgccagac ggggcgtgat gcctctcccg gcagccgcgt tttccccgtt gacgatgatg
     2521 aagtccgccc cgtgttcccg tttgagttcc ggcaacatgt gcttcacggc ggtgcgtccg
     2581 ggttcgccga ccacatcgcc tataaagagt atggtaatca tgattgggac ggagctggcg
     2641 cagatggcgg tttttactgt tcttccctgg tgacgggcac taccagaacg ggcacggggg
     2701 atttccgcat cacggagctt gcgacgctgc ccagaaagac ggtggagagg aagccgtggt
     2761 tatgcttgcc caccacgatc atgtcgatgt ggtgtttttc agcataatta atgactgctt
     2821 cgctgatttc aaattcatct tccaccgcct ggatgatcgt ggcgtccgtc agccgggaga
     2881 gtttttccgc cgtttgtttc aaatggtttt tagcgatgga aataacttcg ttttcttccg
     2941 tctggtccat cacgggaacg ggcacttcat ccggcagaac gccggaaact tcatagcaaa
     3001 tgctgggttc cacaatgtgg agcaaatgga tgatgctgtc gttggggcag gccagtttgc
     3061 cgatttggtg gatgacggcg tccgtggcgt tggagaaatc aatagctacc agaatattct
     3121 tcatatcttt ctgtataaca ttccgtactt ttccatgcca caaaaaaacg tgtgctgccc
     3181 cggctttccc gggtgttccg ttttaggcca gccccacggc gcggcgcacc ctgtccagca
     3241 ctacagaggc cgtctcgcgc gccttgcggg cgccttcttc cagcgtccgg cgcacatagt
     3301 cctgattggc ttccagttcc gccctgcgtt cgcgagcggg ccggaagtat tcccagtaag
     3361 cgtcaaagag gcgctttttg aagtctccat agccgcatcc cccgttctgg aaatcgcgca
     3421 ccatgttttc ataaccctgc gcggaggcaa agagtttgta aagttgcagg atgacggaac
     3481 cttccgtcgg cttgggatct tccacggggg tggaatccgt agggattctc atgatgagtt
     3541 tgcggacagc tttttcctct ccgaaaatag gaagcgtgtt attatagctt ttactcattt
     3601 tctgcccgtc cagccccgga acaatggctg tgctttccgc aataagcgca tccggaagtt
     3661 tgcaggtgcc ttctccgaat tgttcgttca ttttgcccgc caggtcgcgt gtgacttcca
     3721 gatgctgttt ctggtccttg cctacgggca ccaggtcgga gtcatacatc aggatgtcgg
     3781 ccgccatcag cacggggtag gtgaacaggg cgttgctggg ggagaatccc ttggcaattt
     3841 tgtctttgta ggaatggcag cgttccagca ggcccatcgg acagacggtg ctgagtatcc
     3901 aggccagttc attgacttcc ggcacggcgg actggcggaa aaagacgcat ttttcgggat
     3961 ccaggcccac ggccagaaag tccgtagcga ggttgctgca gttttccctc agcgtgttcg
     4021 cgtcgtgaac ggtgctcatg gcgtggtagt ccgccaggaa gtagaaagat tctccgcttt
     4081 cctgcatgcg gacggccggt tccatggctc cgaagtagtt gccgacatgc agcttgccgc
     4141 tgggttgaag acctgtgatg atacgcattg cgcgcgggat tttgcctgtt cgcgcccggc
     4201 aagtcaagaa aggggagagt caaagacagg aggggccgtc ggctggattt ttttccggca
     4261 tgtgcctgcc tcacgagatg cgttcagaag ggatgcctct tcttatttat ggaatgtccg
     4321 gaaagggccg gtcagcgtgg aaaaggatgg gggtaaaatt actcttccag tgccgccagc
     4381 acattgtgga caccgtccgc aaagcttggg tagagcggtt cccagcccag ggccataagg
     4441 cgggagcagg aaatcctctg gttggagaag ccccggcgcg cttccgccgg gagcggttca
     4501 ggctgcggag cgggtttccc cagcaaattg gaaaggtagg agtatatttc tcccatttgc
     4561 atgggggtgc ggtccgtcag gttgtaaatg ccggcaggcg gttcccgcag ggtgcagagc
     4621 aggtgcaggg cggctgcggc atcgtcccgg tggatgtaat tgagccatct gttcatggct
     4681 ccggggaggg ccttcccctt cgtgacgtac tgggagacga gaacacagcg gcccggcccg
     4741 tacagggctc ccagccggac gaccgtgcct cccgctgcca ggaccgtctg ttccgcctga
     4801 ataagaagcc cgttttttcc ggaggaggga tagacattgt gttcctccgt aatccagcgt
     4861 ccgtccgtga ttccatagac ggcggtactg gaacagaaga tgaccggcgt tccggaaaag
     4921 gcttccagcg tgttccgcgt cccattggcg caaaggttgc ggtaggcttc ctctc
//
LOCUS       DAOMSU010000158         4891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1577_length_4891_cov_2.204921, whole genome shotgun sequence.
ACCESSION   DAOMSU010000158 DAOMSU010000000
VERSION     DAOMSU010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4891
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1577_length_4891_cov_2.204921"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    207..237
                     /estimated_length=31
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            492..767
                     /locus_tag="PK077_07315"
     CDS             492..767
                     /locus_tag="PK077_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24148.1"
                     /translation="MLELTFQEQQLQDITGTWDFPSGAVQIKDPKEFRMEGESMTFEY
                     LRSLNDSVHLFHIQETKQKKMVTRINNLLLIIPYDCRGPFQEGVMKP"
     gene            complement(846..1148)
                     /locus_tag="PK077_07320"
     CDS             complement(846..1148)
                     /locus_tag="PK077_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24149.1"
                     /translation="MLEDRRSRSSRKTPDLVADELILILHEAGMLEFNDIFQRTVAVM
                     KEKHMSLGGEEILRLRIYEKLQNLVSAGGLMKKGREYTALPRLMALKSSAGGGRLG"
     gene            1585..2253
                     /locus_tag="PK077_07325"
     CDS             1585..2253
                     /locus_tag="PK077_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HRN24150.1"
                     /translation="MSYNVKNGTGMDGRRDYDRTARVIAEEKPDVVALQELDQGTIRS
                     GGRDTLQELAARTTLTGTYAKAIDYSGGSYGVGILSREKPLSVKRIPLPGREEARVLL
                     MAEFRDYWFCVTHLSLTREDSSASIDMIAALAAKCSKPFFIAGDFNLTPDSEPITRMK
                     KHFILLSDPAQKTFPAENPEECIDYIWMYRGKKTEAFHVKERRVIEAPAASDHRPIKV
                     TVSY"
     gene            complement(2272..2973)
                     /gene="rsmI"
                     /locus_tag="PK077_07330"
     CDS             complement(2272..2973)
                     /gene="rsmI"
                     /locus_tag="PK077_07330"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRN24151.1"
                     /translation="MEKVEYSVYFVPVPIGNRADITLRALDVLRKVDVIACEDTRHSG
                     LLLSHYEIRKPLISMHDHNEQPRAGEIAARAAGGESFAVISDAGMPGVSDPGYRLIQT
                     LKEKGISFTILPGPSAVVTALVGSGLPTDAFFFGGFLPVKSGKKNAVLQTAAEASHTS
                     IFYESPHRIVKTMQALAALDPGIPVCVARELTKTFETYYRGPASRLAAEFAERPPRGE
                     IVLLVGGVNAPRATR"
     gene            complement(3003..3518)
                     /gene="lptE"
                     /locus_tag="PK077_07335"
     CDS             complement(3003..3518)
                     /gene="lptE"
                     /locus_tag="PK077_07335"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HRN24152.1"
                     /translation="MNRIRNVFKLFLPVFCLLAGSCGYQLGGTKPPKLELAQTVKVCL
                     FANNSLEPRAATLVTGALADELQRDGTYRLSSGADADIRVEGKVYSITFDQLRSSRED
                     TYKSTELGLRLSVKYRVVDARTQEILYAGSAEEVGQFFDQGNIQSARTNALSYAARLV
                     ATSIAETLTNG"
     gene            complement(3537..4481)
                     /locus_tag="PK077_07340"
     CDS             complement(3537..4481)
                     /locus_tag="PK077_07340"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN24153.1"
                     /translation="MKKLFLMMAAVSGALALCQCSSEAPPPPGTVRMVDQQAIALMQE
                     ARAREAKNDLSGAIKKYRRVVEKHPLSREAPQARFRMAELYEARKEPAEAFDQYQKLI
                     DRHPDSPLYRQAMSRQKEMAFGAASGALTNRVLWMFDVRMDPTNVTEWLKHVRDNAPY
                     APTAPQAMNVLGNYLAARGRMKEAIEAYQNLVDNYPNSPLAPTAQLQIATLYRQAAAD
                     GDRNHVNVARAQEAYEDYLQRYPNSARAGAARADLAAMKRELVAQQLEVAEYYLTKMK
                     DADAAVFCYQEVASKGSINPAAAARAKARLKELRVTSR"
     gene            complement(4551..>4891)
                     /gene="hisI"
                     /locus_tag="PK077_07345"
     CDS             complement(4551..>4891)
                     /gene="hisI"
                     /locus_tag="PK077_07345"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRN24154.1"
                     /translation="AYMNAESLRMTMELGEAVYYSRSRQEIWHKGATSGHVQKVKQIL
                     VDCDQDALIVLVDQCGAGACHTGHHSCFYRSVPFGDELKAQPQGEPVTLREADGGVVF
                     DAEAVYGKGH"
BASE COUNT         1098 a         1356 c         1377 g         1029 t
ORIGIN      
        1 acggggcgcc aagccaactg ggtgcgtcag gagctgctcg gatattggac ggaacggctc
       61 cataaagcgc attcggccct catggagcat tactcccatg ctccagccat agcaaaaaaa
      121 ttgaaggcgg ctcagaagga atgggaaagt ttccaggact ccctgggagc ggcatatgcc
      181 gcatgtctga cggaagagga tgaaaannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt
      241 aacatgctcc gcctggactg cagtaacacc gagcggcatt gccgcactct ggaagaactg
      301 ctggacatta tcaagatgaa cggtctctaa gcggacgaaa actaccaacc atcaccatat
      361 taaatacata tgaatatcgc aaagacattc agcacttttc ccggacaggc ggagccatta
      421 atattattcg agctgccgca ttcattcttc ttaccgtccc ggcttcagcg caaataccgc
      481 cggaggcaaa gatgctggaa ctgacttttc aggaacagca actccaggac atcaccggaa
      541 catgggactt cccttcaggc gccgttcaaa ttaaggatcc gaaagaattc cgaatggagg
      601 gggaaagcat gacgttcgag tatctgagat ccttaaatga cagtgtccac ctgttccata
      661 tccaggaaac caagcagaag aaaatggtaa cccggattaa caacctgctt ctcattatcc
      721 cttatgactg ccgcgggcca ttccaggaag gggtgatgaa accctgagct tttccgtaaa
      781 agagcctctc ttttacggga aacccatttc agaaaaccgc aacccatgcc tttcccgaat
      841 cagggttacc ccagtctgcc cccgcctgcc gatgatttta aggccatcag cctggggagg
      901 gccgtgtatt cgcggccttt tttcatcagg ccgccggcgc tcaccagatt ctggagtttt
      961 tcgtaaatcc tcagcctcag gatttcctcc ccgcccaggg acatgtgctt ttccttcatc
     1021 acggcaacgg tacgctgaaa aatgtcattg aattccagca tgcccgcttc atgaagaatg
     1081 agaataagct catcagccac caaatccggt gtcttgcgcg aggaacgtga tctcctatcc
     1141 tccagcatga tgataggata ttatcatgac acacttccag ccgtcaagtt gataaaattt
     1201 gcgtcatttc ccgccggcat atggctccaa gagcatccaa ggcggctggc ccgatcaggg
     1261 atgaggccct gctccggaaa ccggaaacga gctgccgcct ctcctcacag gaggattgcg
     1321 tatccggatg gggaaaattt tctgcaagaa aagggagagg ggagctcgta ttatctgcag
     1381 cccgggcaat acggagcttg ttgcaaataa gaaaagattc tctgcaagaa aagggaacag
     1441 ggaacacgta ttacctgcag cccgggcagc aatacgaagc tttttacaga gatgaaagga
     1501 aataaacggt tcattttcag gttatgtgca gccgcagcgc tttttctgaa cctttttccc
     1561 ctgcaggccg gagaatgcac gctgatgagc tataacgtaa agaacggcac cggcatggac
     1621 ggcaggaggg attatgaccg cacggctcgg gtaatcgcgg aggaaaaacc ggatgtcgta
     1681 gcccttcagg agctggacca ggggacaatc aggagcggcg gcagggatac cctgcaggaa
     1741 ttggccgcaa ggacaacgtt gacgggaacc tatgccaaag ccattgatta ttcgggcggt
     1801 tcctatggag taggcatttt atccagggaa aagcctctga gcgtcaaacg gattcctttg
     1861 cccggcaggg aggaggcccg tgtgctgctg atggccgagt tcagggatta ctggttttgc
     1921 gtcacgcatt tgtccctgac cagggaagac agcagcgctt ccatcgatat gattgccgcc
     1981 ttggccgcaa aatgcagcaa gccctttttt attgccgggg atttcaattt aacgccggat
     2041 tcggagccga taacccggat gaagaagcat ttcatcctgc tgagcgatcc ggcccaaaaa
     2101 acgttccccg ccgagaatcc ggaggaatgc attgattaca tctggatgta caggggaaaa
     2161 aagacagagg cgttccatgt gaaggaacgc agggtaattg aagctcccgc cgcctccgac
     2221 caccgcccga ttaaagtgac ggtcagctat tgacatgatg ttttcggtcc gtcaacgggt
     2281 agcccgcggg gcattgacgc cgcccaccag cagcacgatt tcccccctgg gggggcgttc
     2341 cgcaaattcc gccgccagcc gggaagccgg accgcgatag taagtttcaa aggtttttgt
     2401 cagttccctg gctacgcaga cgggaatgcc cggatccaga gccgccagcg cctgcatggt
     2461 tttaacgatg cggtgggggg attcataaaa aatactggta tgggacgctt ccgccgccgt
     2521 ttggagcacg gcgttttttt tcccggattt gacgggcaga aagccgccga agaagaaggc
     2581 gtccgtaggc aatccggacc ccaccagagc cgtcaccacg gcggatggtc cgggaaggat
     2641 ggtgaagctt attccctttt ccttgagcgt ctgaatgaga cggtagcccg gatcgctcac
     2701 gccaggcatg cccgcatcac tgatcacggc aaagctttcc ccgccggccg cgcgcgccgc
     2761 aatttccccg gcgcgcggct gttcattatg gtcatgcatg cttatcagcg gtttgcggat
     2821 ttcataatgg gaaagcagaa ggccggaatg gcgggtgtct tcacacgcaa tgacatccac
     2881 cttgcgcagc acatccagcg cccgcagagt gatgtccgcc cggttgccga taggcaccgg
     2941 aacaaaataa acgctgtatt ccactttctc cataagcaca gattttcctg aaccgggcgg
     3001 aatcagccat tggtaagggt ttccgcaatg gaagtagcca ccagacgggc cgcataggaa
     3061 agggcattcg tgcgggccga ctgaatgttg ccctggtcaa agaattgacc tacttcctcc
     3121 gcagagccgg catacaggat ttcctgcgtc ctggcgtcca ccacacggta tttgacagac
     3181 aggcgcaacc caagttccgt actcttgtag gtgtcttccc ggctggatcg caactggtcg
     3241 aaggtgatgg agtacacttt cccctccacc cggatatccg catccgcgcc ggagcttaac
     3301 cggtaggtgc cgtcccgctg cagctcatcc gccaaggcgc cggtcaccag cgtggcggca
     3361 cggggctcca aagagttgtt ggcaaaaagg cataccttaa cggtctgcgc cagttccagc
     3421 ttgggcggct tggtgccgcc aagctggtag ccgcaagaac cggccagaag gcaaaaaacg
     3481 ggcaggaaga gtttgaaaac gttccggata cggttcatga ggacggagag ttcctgttat
     3541 ctgctggtca cgcgcagttc tttcaggcgg gccttcgccc tggcggcggc ggcaggattg
     3601 atgctgcctt tggaggctac ttcctggtag cagaagacgg cggcatccgc atctttcatc
     3661 ttggtcaggt agtattccgc cacttccagc tgctgggcca ccagctcacg cttcatggcc
     3721 gccaggtcgg ctcgggccgc tcctgcccgg gcgctgttgg gataacgctg aaggtagtct
     3781 tcgtacgctt cctgagcgcg ggccacattg acgtggttgc ggtctccgtc cgcagcagcc
     3841 tggcgataca gggtggctat ctggagctgg gccgtggggg ccagcggaga gttggggtaa
     3901 ttgtccacca ggttctggta tgcctctatg gcctccttca tccggccgcg cgcggccagg
     3961 taattgccca gcacgttcat ggcctgcggg gcggtagggg cgtacggggc gttgtcccgc
     4021 acatgcttca gccattccgt gacattcgtg ggatccatcc tgacgtcaaa catccacagc
     4081 acgcggttgg tcagcgcgcc gctcgcagcg ccgaaggcca tttccttctg ccgggacatg
     4141 gcctgcctgt acagggggct gtccgggtgg cggtcaatga gtttctggta ctggtcaaac
     4201 gcttccgcag gttccttgcg ggcctcatag agttccgcca ttctgaaccg ggcctgggga
     4261 gcttccctgg aaagggggtg tttttccacc acgcgcctgt atttcttaat ggccccggag
     4321 agatcattct tcgcttccct tgccctggct tcctgcatca gggcaatcgc ctgctgatcc
     4381 accatgcgga cggttccggg aggaggaggc gcttccgaag agcattggca cagggccagc
     4441 gccccggaaa cggcagccat catgagaaac agttttttca cattcatgat tttacagcag
     4501 aaaaagaggg cgcgtcaacc cggagaacgc agattgacgc gcaaaaggag ttaatggccc
     4561 ttgccgtaca cggcttcggc atcaaaaacg acgcctccgt ccgcttcacg cagcgtgacg
     4621 ggttctccct ggggctgcgc tttcagttca tcgccgaacg gaacggaacg gtaaaagcag
     4681 gagtggtgcc cggtgtggca ggctccggcg ccgcactggt ccaccagcac aatcagggca
     4741 tcctggtcac agtcaaccag gatttgcttt actttttgca catggccgct ggtagcgccc
     4801 ttatgccaga tttcctgacg gctgcgggaa tagtagacgg cttcccccag ttccatggtc
     4861 atgcgcaggg attccgcatt catataggcc a
//
LOCUS       DAOMSU010000159         4862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1597_length_4862_cov_2.292282, whole genome shotgun sequence.
ACCESSION   DAOMSU010000159 DAOMSU010000000
VERSION     DAOMSU010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1597_length_4862_cov_2.292282"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3192)
                     /locus_tag="PK077_07350"
     CDS             complement(<1..3192)
                     /locus_tag="PK077_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HRN24155.1"
                     /translation="MTDSSIPDMMAEARRGQWTDQQLAAKAVELAESILKQSNAGMRG
                     KEKRQAQQMERMMNDPAGKAFTLALADRVFRPSSPARGAELFRYLLDGYGVPRYLSAA
                     DRFAMKMGGRFSAQFPGVVIPVITSQLRKESSNVILPAEDGKLRPHLRRRRKGGIRMN
                     INQLGEAILGESEAHHRLQQVVDRLTDKDCDYISVKISAIFSQIHLVAFEETVKLIQE
                     RLRILYRAAITNAVTLPDGSRKPKFVNLDMEEYRDLHLTAEAFKRTLMEDEFMQLEAG
                     IVLQAYLPDSWEEQMKLCAWAKERVGQGGARIKIRLVKGANLAMEKVEASMHGWAQAP
                     YGTKAQVDANYKRMLHYGCMPDNARYVQFGVASHNLFDLCYAMLLREREGVRDQVEFE
                     MLEGMANHQARVIRQAADGLLLYAPVVLKEDFHSAIAYLVRRLDENTSEENFLHDLFG
                     MTPGSRSWEVQKKRFLKACQEKDEVKYGPNRTQNRAADPIQPSHYRDAFANERDTDWS
                     LRQNAEWINGLIAAEKEKSGEEIPLVIDGEEITTNLWGVGRDPSRHNEVSYKFAYADF
                     DQVEHALVTADRARSSWASKSIGERAEILHRAAQELSRIRGEAIAAMVRDAGKAPTEA
                     DVEVSEAIDFCRYYAEGLDRDGMNDGVEMSPLGTICVMSPWNFPFAIPTGGVAAALMA
                     GNAVVFKPSELAVYTAWQIVQAFWRAGVPKNVLQFVPMPRNEISRKFLMDPRLNGVIM
                     TGSYRTGKMLRELRPDLHVLAETSGKDAMIITATADPDQAVKDLVKSAFGHSGQKCSA
                     ASVAIVEASVYDNPAFLRQLKDAAASLKVGGSWEVNSVVTPLIREPEGNLLRALTQLE
                     PGEEWLLKPEPSEDNPCLWSPGIRLGVKPGSWFHQTECFGPVLGIIRAENLEEAIDIQ
                     NDSEFGLTGGLQSLDEREIALWKTKVQVGNAYINRVITGAIVRRQPFGGWNHSSMGPG
                     AKAGGPNYLTMLGSWEEKALPQKLRTPGERISGLVEKLCSELPDCAKRIRSAAGSQAK
                     WWMEEFGVDHDPSRVYGENNIFRYTPVKGILARV"
     gene            complement(3351..4328)
                     /locus_tag="PK077_07355"
     CDS             complement(3351..4328)
                     /locus_tag="PK077_07355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24156.1"
                     /translation="MRTLAVIAFLALAFPGRAEMDALSVSCSAAPSLPSPVLVVYGSE
                     EPGSAEARWLKALQPGTEERRALDEYVRDVQFVTVPRHPSVKDWEEDGSLSRAAMHGV
                     RMLPSVVFLDSKGRVFDLVERGASAASLREKVSLLAEKAQRVRPVTRVNDIPKGGDPA
                     AEASSICRELEQVPPEAWFRDYPGTMKRLEKLNCTVPAFLNAREAALRLEKNRKTAEL
                     LGESFRASKASSIRTCLEAWRTCADDPSLSVEERQLILLSMVHPLWVRLEKVLYREAH
                     TPESEDAFNQAVAVLEEVRDMNRSSVCGRRAHQLREELRRARLAAARYD"
     gene            complement(4346..4765)
                     /locus_tag="PK077_07360"
     CDS             complement(4346..4765)
                     /locus_tag="PK077_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRN24157.1"
                     /translation="MKSLLASLGFSADIEVTETDGIVCLNISSPDSQYIIGGDGDRLD
                     DLQYLVNRMIQRKMEDAPRVKVDCDHYRERNEARLLEKARSLAERVQESGKPMKMPPL
                     NAYHRRLVHNALQSMEGIVTESEEGDSRYKRITIRKG"
BASE COUNT         1032 a         1536 c         1264 g         1030 t
ORIGIN      
        1 cacacgggcc agaattccct tcaccggggt atagcggaag atattatttt ctccgtaaac
       61 acgggaggga tcatgatcca cgccgaattc ctccatccac cacttggcct gggaaccggc
      121 cgcggaacgg atgcgcttgg cgcagtccgg cagctcggaa cacagttttt ccaccagtcc
      181 ggagatacgt tcacccggcg tacgcagctt ttggggcagc gccttttcct cccaacttcc
      241 cagcatggta aggtagttgg gacctccggc cttggctcca ggccccatgg aggaatggtt
      301 ccacccgccg aacggctggc ggcggacaat ggcgccggtg atgacacggt tgatgtacgc
      361 gttgcccacc tgcactttag ttttccacaa ggcaatttcc cgttcatcca gggactgaag
      421 gccgccggta aggccaaatt cggagtcgtt ctggatgtca atggcttcct ccaggttttc
      481 cgcacggatg attcccaata ccggaccgaa gcattccgtc tgatggaacc agcttcccgg
      541 tttcaccccc agccggatgc cgggagacca gaggcacggg ttgtcttccg aaggttccgg
      601 cttgagcagc cattcctccc cgggttccag ctgcgtcagc gcacggagca gattcccttc
      661 cggctccctg atgagcggcg tcaccacgga gttgacttcc caggatccgc ctaccttcag
      721 gctggcggcg gcatccttca actgacgcaa aaaggcggga ttgtcataaa cggaagcctc
      781 cacaatagcc acgctggcgg cggaacactt ctgtccggaa tgaccgaaag cgcttttcac
      841 caaatccttt acagcctggt ccggatcagc cgtagcagta atgatcatgg catcctttcc
      901 gctggtctca gccagaacat gcaggtcagg ccgcagttcg cgcagcattt ttccggtgcg
      961 gtaggatccc gtcatgatca cgccgttcaa acggggatcc atcagaaact tgcgggaaat
     1021 ttcattgcgc ggcatcggca cgaattgaag gacgttttta ggcacaccgg cgcgccagaa
     1081 cgcctgtaca atctgccaag ccgtataaac ggccagttcg gacggtttga acaccacggc
     1141 atttcccgcc atcagggcgg cagctacacc gcccgtcgga atggcgaagg ggaaattcca
     1201 gggagacatc acgcagatgg tacccagcgg ggacatttcc acgccgtcgt tcattccgtc
     1261 gcggtccagg ccttccgcgt aatagcggca gaagtctatg gcctcgctca cctctacatc
     1321 agcctccgtg ggagctttcc cggcatccct caccatggca gcaatggctt cccccctgat
     1381 tctggacagc tcctgcgctg cccggtgcag gatttcagcg cgttcgccaa tgcttttgga
     1441 cgcccaggag gagcgggccc tgtccgccgt gaccaaggca tgttcaacct ggtcaaaatc
     1501 cgcataagcg aatttatagg aaacttcatt atggcgggac ggatcgcgcc ccacgcccca
     1561 cagattagtc gtaatttcct caccatctat aaccagaggg atttcctcgc cggatttttc
     1621 cttctccgcg gcaatcagcc cgttgatcca ttccgcattc tgccgcaggg accagtccgt
     1681 atcccgctcg ttggcaaagg cgtcccggta atgtgagggc tgaatggggt ccgcagcgcg
     1741 gttctgcgtg cggttgggtc cgtacttcac ctcatccttt tcctgacagg ccttcaaaaa
     1801 cctctttttc tggacctccc agctccggga ccccggtgtc atgccgaaga gatcatgcag
     1861 gaagttctcc tcactggtat tttcatccag tctccggacc aggtaggcaa tagcgctgtg
     1921 aaaatcttcc ttcaaaacca caggggcata aagaagcaga ccgtccgccg cctggcggat
     1981 gacccgcgcc tgatggttcg ccatcccttc cagcatttcg aattccacct ggtcacgcac
     2041 gccttcccgc tcacgcagca gcatggcata acacaaatca aacaggttgt gggaagccac
     2101 gccgaactgc acatatctgg cattatccgg catgcagccg taatggagca ttctcttgta
     2161 attggcatcc acctgggctt tcgtgccata cggggcctgc gcccagccat gcatggaggc
     2221 ctccaccttc tccatcgcca ggttggcgcc tttcaccagg cgtattttga tgcgcgctcc
     2281 cccctgcccc acgcgctcct tcgcccaggc gcacagcttc atctgctcct cccaggaatc
     2341 cggcaaatag gcctggagca caatccccgc ctccagctgc atgaattcat cctccatcaa
     2401 cgtacgtttg aacgcttccg ccgtcagatg aagatcacgg tattcctcca tatccagatt
     2461 cacgaacttg ggctttctgg aaccgtccgg caacgttacc gcattggtaa tggccgcccg
     2521 gtacaggatg cgcaggcgct cctgaatcaa tttgacggtt tcttcaaagg ctaccagatg
     2581 aatctggctg aaaatggctg aaatcttgac ggaaatatag tcacagtcct tgtctgtcag
     2641 ccggtccacg acctgctgaa gacggtggtg cgcctcgctt tcacccagaa tggcctcccc
     2701 taactggttg atgttcatcc ggatgccgcc cttgcgcctg cggcgcaggt gggggcgcag
     2761 ttttccatcc tcggcaggaa gaattacatt ggagctctcc tttctcaact ggctggtaat
     2821 cacgggaatg accacacccg gaaattgagc ggaaaatctc ccccccatct tcatggcgaa
     2881 acggtcggcc gcagacaaat aacggggaac gccatatccg tccagaagat agcggaacaa
     2941 ctccgcgccc cgcgccggag aagaaggacg gaacacccga tccgccagcg ccagcgtaaa
     3001 cgccttgccc gccggatcat tcatcatgcg ctccatttgc tgcgcctgcc tcttctcttt
     3061 ccctctcatc ccggcattcg actgctttaa aatggactcc gccagttcca cagccttggc
     3121 ggccagttgc tggtcagtcc attggcctcg gcgagcctcc gccatcatgt ccgggatgga
     3181 tgaatctgtc ataacggttc gaagactagc cccggacagg ggcaaaatca agccaatttg
     3241 cccggtcctt cctgtggaaa gaggaatcac ggaagaaaac gcatcccttg attccgtgac
     3301 ggaagcaggg gggccttcct gtccatttcc ggcaactcag gaaagacaca tcaatcatac
     3361 cgcgcagccg ccagacgggc tcttctcagc tcttccctga gctggtgcgc ccgtctgccg
     3421 caaacgctgg aacggttcat gtcccggacc tcttccaaaa ccgcaaccgc ctggttgaag
     3481 gcatcctcgc tttccggagt gtgggcttcc cggtacagaa ccttttccag ccgcacccag
     3541 aggggatgca ccatggagag cagaattaac tggcgctcct ccacggataa cgaggggtca
     3601 tccgcgcagg tacgccatgc ttccaggcat gttcgtatgg aagaagcttt gctggcccgg
     3661 aaagactcgc caagcaattc agccgttttc ctgtttttct ccagacgaag ggccgcctcc
     3721 cgggcgttaa gaaaagccgg aaccgtacaa ttcaattttt ccagacgctt catggtgcca
     3781 ggataatccc ggaaccaggc ttccggaggc acctgttcca gctcccggca aatggaggag
     3841 gcttctgccg cagggtcccc gcccttcgga atatcattca cccgcgtcac cggccgcacg
     3901 cgctgcgctt tttccgcaag cagggacact ttttcccgca gagaagctgc agaggcccct
     3961 ctttccacca ggtcgaacac acgtcccttg gagtcaagaa aaaccacgga aggcagcatg
     4021 cgcacaccat gcatcgccgc acgggacagg ctgccgtctt cttcccaatc cttcacggag
     4081 ggatgtctgg gaaccgtgac aaactgcacg tcacgcacat attcatccag cgcgcggcgt
     4141 tcttccgtgc ccggctggag agctttcaac caacgggctt ccgctgaacc gggttcctct
     4201 gaaccgtaca caaccagaac aggggatgga agggagggag ccgcagaaca ggagacggaa
     4261 agggcatcca tttccgcacg tcccggaaac gccagcgcca aaaatgcaat gaccgctaaa
     4321 gtcctcatga ataaactgcc atccatcagc cctttctgat ggtaatgcgc ttgtaacggc
     4381 tgtccccttc ttcggactcc gtcacaattc cttccatgga ctgaagggca ttatgcacca
     4441 ggcggcggtg gtaggcattc aacggaggca tcttcatcgg cttgccgctc tcctggacgc
     4501 gttcggccag ggaacgggcc ttttccagca aacgggcttc gttgcgctcc cggtaatggt
     4561 cgcaatccac cttcacgcgg ggagcatctt ccatcttgcg ctggatcatg cggttgacca
     4621 gatactgaag gtcatccagc ctgtccccgt cccctccaat gatgtactgt gaatccgggg
     4681 aggaaatatt caggcacacg atgccgtccg tttccgttac ttcgatatcc gcggaaaacc
     4741 ccagcgaagc cagaaggctc ttcaaactct gttcagctat ttcttgcacc gtcattactt
     4801 taaattaaca ggaaaaaggt tccataccgg aaaaaccgtc tcgcgccgga cggcgcgggc
     4861 ct
//
LOCUS       DAOMSU010000160         4843 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1603_length_4843_cov_2.672515, whole genome shotgun sequence.
ACCESSION   DAOMSU010000160 DAOMSU010000000
VERSION     DAOMSU010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1603_length_4843_cov_2.672515"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1172)
                     /locus_tag="PK077_07365"
     CDS             complement(<1..1172)
                     /locus_tag="PK077_07365"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24158.1"
                     /translation="MKTKSRYFFSNYCSDVLWLIRMMSVYFLLWGTIFPAMGRELLPL
                     DGLWELSLPFKQEKVAVVLPGTMDEWGKGNVNNNRQETKHLSRAVTYEGEALYRKEVN
                     VPETFKGKRLFLHVERTKKTTVFVDGKKIGSCDNVQTPHRYDLTEFLFPGKHTLTISV
                     DNSRRHYPAGVFNSHAFTEHTQTNWNGMLGKIFLEAVSDPFVESVKLVPEPEKKSVTV
                     CLTIRNSYKPETARITLEAALQNNGNKGKVERKVIEVPLKLGDQHVKLNYPMGEEPEL
                     WSEFSPNLYKMHIKLELAGKVDTTSKVFGMRCFAVQGTNFTINGKKIFLRGRHDACVF
                     PLTGYPPMDKKAWLRHLGIAAAYGFNYVRFHSWTPPEAAFEAADELGMYLQPEIPF"
     gene            1804..4071
                     /gene="pflB"
                     /locus_tag="PK077_07370"
     CDS             1804..4071
                     /gene="pflB"
                     /locus_tag="PK077_07370"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: protein motif:HMM:TIGR01255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HRN24159.1"
                     /translation="MISIVKDLKESTALPKEWQGFKPGAWTESIDVRDFIQHNYTPYS
                     GNEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEV
                     VVGLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYT
                     SDIRAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDRLIAERRKDLKNREHSPLTDE
                     LIRLREEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAA
                     MSLGRVSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDP
                     TWVTESIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAK
                     VSIETSSVQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNG
                     GVDELKGKQVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCY
                     ERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFP
                     CYGNNDPRVDDIACSLVSNFMEKLRKLHTYRNSLPTQSILTITSNVVYGKKTGNTPDG
                     RRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERE
                     RRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLT
                     REQQQEVINRTFHTR"
     gene            4088..>4843
                     /gene="pflA"
                     /locus_tag="PK077_07375"
     CDS             4088..>4843
                     /gene="pflA"
                     /locus_tag="PK077_07375"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HRN24160.1"
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGC
                     SLRCRYCHNPDTSYVRRGRLRSAGDVLREIARYRDFLQAAGGGVTLSGGDPLFQPDFT
                     GAVLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPERYRSLTGGELR
                     PTLEFAERLASLRKPVWLRYVLVPGVTDNLEEIEGLSRHAALLGNVERVDVLPFHQMG
                     RFKWDELKLDYTLKGTPEASAKLAEQVRGIFRRE"
BASE COUNT         1175 a         1386 c         1191 g         1091 t
ORIGIN      
        1 cagaatggta tttcaggctg aagatacatt ccaagttcat ccgcagcttc aaatgcagct
       61 tccgggggag tccatgaatg gaatcttaca taattgaatc cataggccgc cgctatacca
      121 agatggcgga gccatgcctt tttatccatg ggagggtatc cagtcagggg aaaaacgcag
      181 gcatcatgcc gacctcttaa aaaaatcttc ttcccattga ttgtaaaatt agttccctga
      241 acagcaaaac agcgcattcc gaagacttta ctggttgtgt cgacttttcc tgccaattcc
      301 agtttgatgt gcattttgta taaattcgga gagaattcgc tccagagctc cggctcttct
      361 cccatggggt aattcaattt cacatgttgg tctccgagtt taagaggaac ctcaatgact
      421 tttctctcca cttttccctt atttccgtta ttctgcaaag ccgcctccaa tgtaatacga
      481 gccgtttccg gcttataact gttgcgaata gtcaggcaaa cggtaacgga ttttttttct
      541 ggttcaggaa ccagcttaac cgattcaacg aagggatcag aaacggcctc aaggaatatt
      601 tttcccaaca ttccattcca attcgtctgg gtatgctcgg taaaagcgtg tgaattgaaa
      661 acgcctgccg gataatgcct gcggctgttg tccacagaga ttgtcaatgt atgtttcccc
      721 ggaaaaagaa attccgtcag atcataacga tggggtgttt gaacattatc acaggaaccg
      781 atttttttac catctacgaa aacggttgtc tttttcgtcc gttccacatg caaaaataac
      841 cgtttacctt tgaacgtttc cggaacattc acttcctttc tgtacaaggc ctctccttcg
      901 taagtaacgg cgcgggaaag atgttttgtc tcctgacggt tattattgac attccccttg
      961 ccccattcat ccatggttcc aggtagtaca acagcaactt tttcctgttt gaatggaagg
     1021 gaaagttccc ataacccatc caggggtagc aattctcgtc ccatcgctgg gaaaatagtc
     1081 ccccaaagca ggaaatatac agacatcatc cgaataagcc ataaaacatc ggaacaataa
     1141 ttggaaaaga agtatcttga ttttgttttc attataagaa tttgcatttg agaaaccgta
     1201 cggctcagtc attcgaccac ttgcacattg gaaatacgcc cttgtcacca ttgatttgag
     1261 agctccctaa aacatttggt tagtaatacg aagaactcct tccattttat caaacaacct
     1321 gaataccgtg acaacgaaag gcaaaaaagc atggattctt accgccgaca tgggaaacaa
     1381 ccacacctct ttgaatacgt tttgccgaaa actgtcagaa gagcttctga cagatatttt
     1441 cacaaaggta ttcattgata gctctaccgg aaaaacaatt gattggtact tcatcggcaa
     1501 ctcatttctt tgcttttttc ttttatatct tttcctattt ttctcgtttt cagatgagct
     1561 cccatgaaaa aagatgcccc cctacaagac tcttcagaag gccccattct gtttttccat
     1621 aaaaaatata catatctccg aaatgaacca gaaaaggtaa agaacagttc cctctcccag
     1681 aagaattagc cttgttgtct cccggaattc acgatataat tacgtggtgc ggagttttgc
     1741 cgtttccggc ctttcaacgg ctgcggaacg tttttctccg gtcttcttca ctacatccta
     1801 tttatgatca gcatagtaaa agacttgaag gagtccaccg cccttccgaa ggaatggcag
     1861 ggattcaagc cgggagcatg gacggagtcc attgacgtac gggatttcat ccagcataac
     1921 tacacgccct attccggcaa tgaagaattt ctttccggcc cctcccagcg cacgctccgt
     1981 ttatgggatg aactgaaagt cctgctgaaa agggaaatag acaacggcgg cgttctggat
     2041 gctgatgaag aagtggtatc ctccatcacc tcccacaagc ccggctacat tgacaaggaa
     2101 ctggaagtgg tcgtaggcct ccagacggac gcccccctga aacgggccct gatgcccttt
     2161 ggcggcctgc gcatggccca gcaggccctg gaatcctacg ggttcaagat gtgcgagaaa
     2221 accgcggata ttttcaaaaa gatacgcaag acgcacaatg aaggcgtttt cgacgcctac
     2281 acttccgata tccgggcggc ccgttccgcc ggcattatta ccggccttcc ggacgcctat
     2341 ggccgcggcc gcatcatcgg ggactaccgc cgggtggccc tgtacggcac ggacagactg
     2401 attgccgaac gccgcaagga cctcaaaaac cgtgaacatt ccccccttac ggatgagctg
     2461 atccgcctgc gcgaagaaat gagcgaacaa atacgcgccc tggaagaact cgcccagctg
     2521 ggcgcctcct acggctgcga cctcactcgc cccgccgcaa acgcccgcga agccgtgcaa
     2581 tggacgtacc tgggctacct ggccgccgtg aaggaacaga acggagcggc catgtccctg
     2641 ggccgcgttt ccactttctt cgacatttat ttcacccgcg acctggaaca ggggctcatc
     2701 acggaagagg aagttcagga aatcatcgac cagttcgtga tgaagctgcg catcgtccgt
     2761 ttcatccgca cgcctgatta caataacctg ttctccggag accccacctg ggtgacggaa
     2821 tccatcggcg gcatgggaga ggacgggcgc accctcgtca cccgcagttc cttccgcatg
     2881 ctgcagaccc tgtacaacct ggggccggct ccggaaccga acctgaccgt tctgtggtcc
     2941 cggaacctgc cggaagcctt caaaagcttc tgcgccaagg tttccattga aacgtcctcc
     3001 gtccaatacg agaacgacga cctgatgcgc ccgcactggg gcgacgacta cggcatcgca
     3061 tgctgtgtgt ccgccatgcg catcggcaag cagatgcagt tcttcggagc gcgcgccaac
     3121 ctcgccaagt gccttctcta cgccctgaac ggcggcgtgg atgaactcaa gggcaaacag
     3181 gtggccccgc cctccccccg ctacacggaa gaaattctca actatgatga agtgatggcc
     3241 ctgtacgaca agatgcagga ctggctcgcc aaaacttaca ttgacgcgct gaacatcatc
     3301 cactacatgc acgacaaata ctgctatgaa cgcattgaaa tggcgctgca tgatccggaa
     3361 atcctgcgca cgatggccac gggaatcgcc gggctttccg tggcggcgga ctccctgtcc
     3421 gccatcaagt acgccaccgt aaaagccatc cgcaatgaag aggggctgat tgtggacttc
     3481 aagacggaag gggaattccc ctgttacggg aacaatgacc cgcgcgtgga cgacatagca
     3541 tgcagcctgg tcagcaattt catggaaaaa ctgcgcaagc tccacactta ccgcaattcc
     3601 ctgcccaccc agtccatcct gacgatcacc tccaacgtgg tgtacggcaa aaagacgggc
     3661 aacacgccgg acggccgccg agccggggaa ccgttcgccc ccggcgccaa ccccatgcac
     3721 ggaagggaca ggaacggagc cgtggcgtcc atgctctccg tagccaagct gtcctatgac
     3781 gactccctgg acggcatctc ctacaccttc tccatcgttc cgcaggccct gggcaaggag
     3841 gaaagggaac gccgcgtcaa gctcgtctcc ctgctggacg cctactttgc cgctacaggt
     3901 caccacatta atgtgaacgt actggaacgg gaaacgcttc tcgacgccat ggatcacccg
     3961 gaaaaatacc cgcagcttac catccgcgtt tccggctatg ccgtgaattt catcaagctg
     4021 acccgggaac agcagcagga ggtcatcaac cgcaccttcc acacccgcta acccggacga
     4081 cacacggatg ccgcaggttt ttccacaaaa ccatgatccg gacggagaaa gctccgtcac
     4141 cggtttggtt cattccgtgg aatcctgcgg caccgtggac ggtcccggca tccgctttgt
     4201 ccttttcctg tccggctgca gcctgcgctg ccgctactgc cacaatccgg atacctccta
     4261 cgttcggcgg ggccggctca gaagcgcggg tgatgtcctc agggaaatag cccgttaccg
     4321 ggattttttg caggcggcgg gcggcggcgt caccctctcc ggcggggacc ccctcttcca
     4381 acctgatttt accggcgccg tgctgaaagg ctgccggaaa ctgggactgc atacctgcct
     4441 ggacacctcc ggccatctgg gcgtgaatgc gggcgaagaa atgctggcgg atacggacct
     4501 ggtcctgctg gatatcaagg catggaatcc ggaacggtac cgctccctga caggcgggga
     4561 actgcgccct acgctggagt ttgcggaacg cctggcctcc ctccgcaagc ccgtgtggct
     4621 gaggtatgtg ctggtacccg gcgttacgga taatctggaa gaaattgagg gactctcccg
     4681 ccatgcggcg ctcctgggca acgtggaacg cgtggacgtg ctccctttcc accagatggg
     4741 gagattcaaa tgggatgaat tgaagctgga ttataccttg aagggcaccc cggaagcgtc
     4801 cgcaaaactt gcggaacagg tccgcggcat tttccggcgg gaa
//
LOCUS       DAOMSU010000161         4839 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1609_length_4839_cov_2.661162, whole genome shotgun sequence.
ACCESSION   DAOMSU010000161 DAOMSU010000000
VERSION     DAOMSU010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1609_length_4839_cov_2.661162"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            178..1302
                     /locus_tag="PK077_07380"
     CDS             178..1302
                     /locus_tag="PK077_07380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN24161.1"
                     /translation="MPSPLISVIVPIYNMESLLPRCLDSLAAQTLRDLEIICVDDGST
                     DGSGGIVRKYASGDSRFRLITQENSGRAEARNAGIRAAAAPYLGFADPDDYVEPDMYE
                     RLYRLAEESGADMVQCSYSPFLPAESGESRGMAEEKLLHIENTACDGVFTEKGEIFRL
                     FLEDRITGVVWSKLFRRILPGCSAPLEVRLPSSFTSGEDTLYVSRAIARCRSVALTSE
                     KLYHYGLGGPQSVSSRNRKAETRPASYYAVFEMLTREKLREGVLGSNRTAYMNYIVPL
                     LFPDNEMPAGRLRHWAELWREADITSEHVCGMPREQRAYLEAALAGRWIYLRLLQCFW
                     KFKTARRKMFRLRFSKNGIDTLQILGYTLYSAKKLPRSSL"
     gene            1317..2666
                     /locus_tag="PK077_07385"
     CDS             1317..2666
                     /locus_tag="PK077_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRN24162.1"
                     /translation="MFIYSYQQHDHLLPGNDRMLVIGSSALAKNHGHLKSSRLAADTL
                     IFNFPLNHPRLWRQMEHSLSGARYSIILFMWPPLHLSPANGRLMEKPPEGGGPMESAE
                     DMLCRLFAFLRKQTRRLILLSSPPSDYGSARHPYAEEPGVLEAWNALLRTLADRLNLP
                     FIDFCGDMLQCRPLPGSGEEDFPEAYRNLGKQVLRRLPFGQTWVRRRSGKIHRLLEKR
                     RKRRKERMLARWRTLAAPPHYREETDWITAIHSEKGQARILLIGDSVMRHLWRPLASE
                     LQEDIDLFSSTLIPGDPDYLPTLAGYFPSAGYEAVIVSFGSHVTNKVSAISVDDFRKN
                     YMAFASQLSKRCRFLILATSTSIMDGPDGTLNEEKEKIITAFNAIVRETASSLPGAVL
                     SDHHDFMQGARYIDPFHFSPPDRAYQAGKTAGLLLPLLSARQEPPKEQECPKTRPEP"
     gene            2897..3994
                     /locus_tag="PK077_07390"
     CDS             2897..3994
                     /locus_tag="PK077_07390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HRN24163.1"
                     /translation="MTTPPVPASSEKSRIPVMFSATGGWGLPLGVAIHTLCLHAGSGR
                     FYDIHIVHDGMDARIIQELNRVAAPFTQVSLSFLQLPEEFRQLFQNGNKDRYSPLAYA
                     RLMAGSLFPQYGRIVYLDADVLLAGDVAELYFSDLRGASVAAAGDGLALWSIEKGTMH
                     PHLEYMGNYLSSPLSYCNSGVLVLDLDQMRRRNLEHRLLQWLRSRPEPFPYPDQDILN
                     IALHGDITPLPPEWNFQFLSWTWDEERIQRLRGTEFENVPSISCGHAWKLLHMVGPEK
                     PWRLPDAPGTIGQFHWILYSFFWWQEAKKLPAFRKELDAISRGLAPLIRRHIRSQQWK
                     LFFSRGHIFRRRRDKIRWLKKVLSILDGRKP"
     gene            complement(4105..>4839)
                     /locus_tag="PK077_07395"
     CDS             complement(4105..>4839)
                     /locus_tag="PK077_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HRN24164.1"
                     /translation="YNETTAKIYTDISYLTCDDQLGADASQFFNSVTGRTKLMRFRKL
                     YPSPVLMKARLIELIEGEAERARQGEPARISAKMNSLQDVEIIDALYRAADAGVDIEL
                     NVRGICCLKTGPRPNGKVIRVISIVDRYLEHARIFFFHHGGNHQLFIASADWMTRNLD
                     KRVELMVPVTNGKLARRLKSILDACFRDNVQACNILPDGTSEHVVRKKGQKAFRLQQY
                     LTKEARRLAKDKERERQQMLEPHTPH"
BASE COUNT         1018 a         1546 c         1254 g         1021 t
ORIGIN      
        1 catcgccaac agcactttct cctggtgggc ggagtggctc aacgaacacg cggagaaaat
       61 cgtcatcgcg ccgcgcatct ggttcaacag ggaggaaggc gaccggtacc atacggatga
      121 cgcactcatc cccggctcat ggctccggat ataaccctgt ttcctataca gcagaccatg
      181 ccctctcccc tcatttccgt catcgtcccc atctacaaca tggagtccct tcttccgcgc
      241 tgtctggaca gcctggcggc acaaaccctc cgcgacctgg aaatcatctg cgtggacgac
      301 ggttcaaccg acgggtccgg aggcatcgtc cggaaatatg cttccggaga cagtcgtttc
      361 cgcctgatta cacaggaaaa ttccggcagg gcggaagccc gtaacgcggg aatcagggct
      421 gccgcggcgc cttacctggg ttttgcggat ccggatgact acgttgagcc ggacatgtac
      481 gaacggctct accggctcgc ggaagaaagc ggggcggaca tggtccaatg ttcctattcc
      541 cccttccttc cggctgaaag cggagaatcg cgcggaatgg ctgaggaaaa actccttcat
      601 attgaaaaca ccgcctgcga cggcgtcttt acggagaaag gagaaatatt caggctcttt
      661 ctggaagaca ggatcaccgg cgtcgtctgg agcaaattgt tcaggcggat actccccggc
      721 tgttccgccc ccctggaagt ccgcctgcct tcttccttca ccagcggaga agacaccctt
      781 tacgtttccc gggccatcgc ccgctgccgc agcgtcgccc tcacctctga aaaactctac
      841 cactacgggc tgggcggtcc ccaatccgtt tcttctcgca accggaaagc ggaaacccga
      901 cccgcaagtt attacgccgt attcgagatg ctcaccaggg aaaaactccg ggaaggggtt
      961 ctgggcagca accgcacggc ctacatgaat tatattgtcc ccctgctttt tccggacaat
     1021 gaaatgccgg ccgggcggct caggcattgg gcggaactct ggcgggaggc ggacatcact
     1081 tccgaacacg tttgcgggat gcccagggaa cagagggctt atcttgaggc cgccctggcc
     1141 ggacggtgga tttacctgcg ccttcttcaa tgcttctgga agttcaagac cgccagaagg
     1201 aaaatgttcc ggctgaggtt ttccaaaaac ggaatagaca cgctccaaat cctcggatac
     1261 actctttatt ctgcaaagaa gctcccccga tcatccctgt aactgctgtt tttcccgtgt
     1321 tcatctattc ctaccagcaa cacgaccacc tcctgcccgg caatgacaga atgctggtga
     1381 tcggcagttc cgctctggca aaaaaccatg gccatctgaa atcgtcccga ctggccgccg
     1441 acacgctcat cttcaatttc ccgctcaacc atccccgcct gtggcggcaa atggagcaca
     1501 gcctttccgg cgcccgctac agcatcattc ttttcatgtg gccgcccctg catctttccc
     1561 cggccaacgg caggctcatg gaaaagcccc cggaaggggg cgggcccatg gaaagcgccg
     1621 aagatatgct ttgcaggctt ttcgcctttc tgcggaaaca gacgcgccgc ctgatcctcc
     1681 tgtcatcccc gccctccgac tacggcagcg cccggcaccc ctacgcggaa gaaccgggcg
     1741 tccttgaagc ctggaacgcc cttcttcgca cgctggcaga caggctgaac cttccattca
     1801 ttgacttttg cggcgatatg ctgcaatgcc gccccctgcc cggctccggg gaagaagatt
     1861 tcccggaagc ttaccggaat ctgggaaaac aggtcctccg ccggctgcct ttcggccaaa
     1921 cgtgggtgag aaggcgttcc gggaaaatac accgcctgct ggagaaaaga agaaaacgcc
     1981 ggaaggaacg catgcttgcc cgctggagga cattggcggc ccctccccat taccgtgagg
     2041 aaaccgactg gattacggcc attcattcag aaaaagggca ggcgcgcata ctgctcattg
     2101 gcgattccgt catgcggcat ttgtggcggc cccttgcctc agaacttcag gaagacattg
     2161 acttgttctc ctccacgctg attcccggag atccggacta tctccccact cttgcaggat
     2221 attttccctc cgccgggtac gaggccgtca ttgtttcctt cggttcccac gtcaccaata
     2281 aagtctccgc catttccgtg gatgacttcc gcaaaaatta catggcattc gcctcccagc
     2341 tcagcaagcg ctgccgtttc ctcatcctgg cgaccagtac gtccatcatg gatgggccgg
     2401 acggcacgct gaatgaagaa aaagagaaaa tcatcaccgc ctttaatgcc attgtccgcg
     2461 agacggcatc ttccctgccc ggcgcggttc tttcagacca ccatgacttc atgcaggggg
     2521 cgagatatat agatcccttc catttctccc ctccggaccg cgcgtaccag gccgggaaaa
     2581 cagccggact gctcctgccc cttctctccg cccggcagga accgccaaag gaacaggaat
     2641 gtccgaaaac ccgccccgaa ccatgaaacg gacaaatgaa cgaaaaccgc caaattattt
     2701 tccatacggg gagaaacggt tacctcccgc aaaaatcccg gccccggcat ttaaagactt
     2761 caaattcccc ccccttcttc aaattacaga catccctttt ccggcctgaa cggagcctcc
     2821 atgctctttc cctccccggc ctatttcttc ccgcagcccc ttcttcaaga cccttcccat
     2881 gaaattcccc gatgccatga caaccccgcc cgttcccgca tcctcggaga aatcacgcat
     2941 ccccgtcatg ttttccgcca ccggcggctg gggcctgccc ctgggcgtag ccattcacac
     3001 cctttgcctt cacgccggtt ccggacgctt ttatgacatt cacatcgttc acgacggaat
     3061 ggacgcgcga ataatacagg agctgaaccg ggtcgccgcc ccctttacgc aggtttccct
     3121 ttctttcctg caacttccgg aagaattccg ccagctcttt caaaacggca acaaggaccg
     3181 ctactccccc cttgcgtatg cccgcctgat ggccggcagc ctgttcccgc agtacggcag
     3241 gatcgtttat ctggacgcgg atgtcctgct ggccggagac gtagccgaac tgtatttttc
     3301 cgatttgcgg ggagcttccg tagcggcggc cggagacggc ctggccctct ggagcattga
     3361 aaaaggaacg atgcaccccc atctggaata tatgggcaac tacctttcct cccccctttc
     3421 ctactgcaat tccggcgtcc tggtgctgga tctggaccag atgcgccgcc gcaacctgga
     3481 acaccgcctg ctccaatggc tccggagccg cccggaaccc ttcccctatc cggaccagga
     3541 catcttaaat atcgccctgc acggagacat aacgccgttg cctccggaat ggaacttcca
     3601 attcctgtcc tggacctggg atgaagaaag aatacagcgc ctgcgcggaa ccgaatttga
     3661 aaacgttccg tccatatcct gcgggcatgc atggaaactg ctgcatatgg tcggcccgga
     3721 aaaaccatgg cggcttcctg acgcccccgg aaccataggg cagttccact ggatcctgta
     3781 ctcctttttc tggtggcagg aagcaaagaa gcttcccgcg ttccggaaag aactggatgc
     3841 gatttcccgg gggctggccc cgcttatccg gcgccatatc cgcagccagc aatggaaact
     3901 gttcttctcc cggggccata ttttccggag acgccgggac aaaatcagat ggctgaagaa
     3961 agtgctgtcc attcttgacg gcagaaaacc gtaagcagct ttcctactct ccggaaaacg
     4021 cgggcagtct ccgcaggcgg ccggaaaacc gggtactccg gagactgttc cttgcgaacc
     4081 tccaaacgcc cgtgacgagc gcctttaatg cggcgtgtgc ggttccagca tctgctggcg
     4141 ttcccgttcc ttgtccttgg ccaggcggcg cgcctccttg gtcaggtact gttgcaaacg
     4201 gaaggctttc tggccttttt tccggacgac atgttcggaa gtgccgtccg gcagaatatt
     4261 acacgcctgg acattgtccc tgaaacaggc gtccagaatg gatttcaggc gacgcgccag
     4321 cttcccgttg gtaacgggca ccatgagttc caccctttta tccagattcc gggtcatcca
     4381 gtccgcgctg gcgatgaaaa gctggtggtt gccgccgtgg tggaagaaga aaatgcgcgc
     4441 gtgctccaga tacctgtcca caatactgat tacgcggatt accttgccgt tgggccgggg
     4501 gcccgttttc aggcagcaaa tgccgcgcac gttcagttca atgtccaccc cggcgtccgc
     4561 ggccctgtac agggcgtcaa taatttccac gtcctgaagg ctgttcatct tggcggaaat
     4621 gcgggcgggt tccccctgcc gcgcgcgttc cgcctcccct tcaataagtt caataaggcg
     4681 cgccttcatc aacaccgggg agggatacag cttgcggaag cgcatgagct tggtgcgccc
     4741 cgtcacggaa ttgaagaact gggaggcgtc cgctcccagc tggtcgtcgc aggtaaggta
     4801 ggaaatatcc gtgtaaatct tggcggtggt ttcattgta
//
LOCUS       DAOMSU010000162         4805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1622_length_4805_cov_2.988000, whole genome shotgun sequence.
ACCESSION   DAOMSU010000162 DAOMSU010000000
VERSION     DAOMSU010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1622_length_4805_cov_2.988000"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PK077_07400"
     CDS             <1..388
                     /locus_tag="PK077_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24165.1"
                     /translation="KFDVKYLPKIVQKYLFQCFLINQMIINKSERNYFKTVILLNKKK
                     VIENQFYNEMKKHGYDWNSRSILAHFIGKNPEDVLKRLVTDSFEKIAGNRTIAIDPKD
                     KRTMEIIIKNAYVEFANYLDDESQKK"
     gene            816..1700
                     /locus_tag="PK077_07405"
     CDS             816..1700
                     /locus_tag="PK077_07405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN24166.1"
                     /translation="MMNLGVTVHEGYDWEAIPAGAPVISFCNEEFLAALPKIRKRTRR
                     TVFVNCMTWLFGREKEAMKRGDISLFLYQNSNVMGNVIPRLRALNPDPAIRFMTFRPY
                     FDAADFPFAAGRSTDWFGAGHISRQDEDKFSKDTWHIYEHFASPVRKRGTFLGFDQRS
                     EVKTGRPPDWVETFHDQKSLSQQEFYRRCHIVLQPTDTTENWPRVGFEAMASGSVLVV
                     DRRGGWEQMVEHGKTGWLCECPGDFITYATKMAWEPHYREDMAAAARERGMALGGQEA
                     SMESWQEVFEAISRIPDS"
     gene            complement(1749..2720)
                     /locus_tag="PK077_07410"
     CDS             complement(1749..2720)
                     /locus_tag="PK077_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24167.1"
                     /translation="MFLAPYAPDAPDFSKKPYTGNGGYPQYHYNIYKAIQDIGYDVVS
                     SSKPYSVQFAKGNVDYVFSLMNRFAMSRPEIFISSYCEFIQVPYLGAPPNIRAIAEDK
                     FLTKMVFRSLELNVPEGIGLSGEKGIPAQAPFPGPYFVKKRFGAASGGIREDSICSSW
                     KAVGSCARRLMEEGHEVLVEQYCEGIDVTVPVLGGENPVILGYVQPKSDKPGSIITED
                     LKLHDHLGYQLVSVQDMEQDIACDVRKIWSALGPMDYFRIDYRIDFETGERRILEMNI
                     CCHLGKSGSICLAAAEHGYSQEDLLKYIIAYSMDRQKNLRQYGTWLI"
     gene            complement(2904..4061)
                     /locus_tag="PK077_07415"
     CDS             complement(2904..4061)
                     /locus_tag="PK077_07415"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HRN24168.1"
                     /translation="MTAPLSVTTASDQLLCRIAGIGDLFAIEGEFVTGKEIPSGHINT
                     TYKATYRKSDGTEDSYILQRINDYVFKDPKAVMRNVEKVTRHINWKVLRRLKDSAGQT
                     LNLYPARGGRNYIDIPGDGIWRCYNYLAGTHTYDVVENTRQAYQAGFAFGSFQDLISD
                     MNPDDIVETIPGFHHTRNRFNRLMEVAELDPQGRLSTCLPELEFIKAREQDVDRLLNL
                     QERGVLPTRITHNDTKINNVMLDEDTDHAVCVIDLDTVMPGLVLYDFGDMVRTVTPPT
                     EEDEEDLDKVRMRMPMFQSIVEGYLTAAHGFLTQAEIDQLAFSGKLITLEIGIRFLTD
                     YLEGDPYFKVSRPNHNLIRCRTQLKLVECIEQELPAMEQYVQRVARGMSRK"
     gene            complement(4094..>4805)
                     /locus_tag="PK077_07420"
     CDS             complement(4094..>4805)
                     /locus_tag="PK077_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_022197812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HRN24169.1"
                     /translation="GIELEKIADLTEIGTRFTLCQGGLGGKGNWHFRSATNQAPTEAE
                     MGTEGEEGVFFMELRRIADAGLVGYPNAGKSTLLGDISEAKPKVASYPFTTLQPIIGV
                     VEFDSFRRCIVADIPGIIEGAHNNRGLGHEFLRHITRCKVLVFVLDMAGSEGRDPIED
                     LQNLRTEIKLYSEDLAKQPWFVVANKMDLEGAEENLVNFRMRFPKVDVIPISALNGDG
                     ISRLRNRLDELVGYKFIR"
BASE COUNT         1129 a         1074 c         1327 g         1275 t
ORIGIN      
        1 gaaattcgat gttaaatatt taccgaagat agttcaaaaa tatctttttc agtgttttct
       61 tataaatcaa atgataatta acaagagtga acgtaattat ttcaaaaccg tgattttgtt
      121 aaataaaaag aaagtaattg aaaatcaatt ttataatgaa atgaagaaac atggatatga
      181 ttggaatagt agaagcatat tggctcattt cataggaaaa aatccggaag acgttctgaa
      241 aagattagtt acagacagtt ttgaaaaaat tgccgggaac agaacgatag ctattgatcc
      301 caaagataaa agaacgatgg aaataatcat aaaaaatgca tacgttgaat ttgccaatta
      361 tttagatgat gaatctcaga aaaagtaatt aatttcttga aacaaaagtt atctgataaa
      421 gaagagaatt aaattgaata ggttttttat tggttgcatc tttttgtgga gatcgatgtt
      481 ttagtttaag tgatttagtg caaattttgc aataaatgtt tattgattaa tggaattgtt
      541 tactttaaat ttgttttatt cattttttct attttcatct gaataatttt ctgaatatca
      601 atagtccaaa tgttctgaaa atcttacggc aagactattc tacataaatc cattaattaa
      661 tgatacattc tctctatatt gttggatttc catcccttta cggcggtgcg ggagccgagc
      721 tttatcacca gattaaggtt tggagacaga tggggatgga aatgcatctt atccccaccc
      781 agaaaaatgc atactgtgcc gggctttatc cggagatgat gaatttgggt gttacggtgc
      841 atgaaggcta tgattgggag gccattccgg caggggctcc cgtcatcagt ttttgtaatg
      901 aagagtttct ggctgctctc ccgaagatac gaaaacggac gagaaggaca gttttcgtca
      961 attgcatgac ctggcttttc ggcagggaaa aggaagccat gaagcgtgga gatatttccc
     1021 tgtttcttta tcaaaatagc aacgtaatgg gaaacgtcat tcctcgcctg agggctttaa
     1081 atcccgaccc ggccatccgg ttcatgacgt ttaggccgta ttttgatgcg gcagattttc
     1141 cttttgctgc agggcgttcc acggactggt ttggagccgg acacatttcc cggcaggatg
     1201 aagataagtt cagcaaggat acgtggcata tttacgagca ttttgcttct ccggtccgga
     1261 agaggggaac ttttctcggt tttgaccagc ggagcgaggt gaagactggc aggcctcccg
     1321 attgggtgga gactttccat gatcaaaagt ctttgtccca acaggagttt taccggcgct
     1381 gccacatcgt tttgcagccc accgatacga cggagaattg gccgcgtgtg ggttttgagg
     1441 ccatggcaag cgggagtgtg cttgttgtgg acaggcgcgg agggtgggaa caaatggtgg
     1501 aacacgggaa gaccggatgg ctgtgcgaat gccccggtga tttcatcacg tacgccacta
     1561 agatggcttg ggagccccac taccgggaag acatggccgc tgccgcccgt gagcggggca
     1621 tggccctggg cgggcaggaa gcttccatgg aaagctggca ggaggtgttt gaggcaatca
     1681 gccggatacc tgattcctaa ctgccgtttt tgttctgacc ggaatgcgaa ccgcttcttt
     1741 cctgcgggtc agatgagcca tgtgccgtat tggcggaggt ttttctgcct gtccatgctg
     1801 taggcgataa tgtatttgag gaggtcctcc tgggagtagc cgtgttctgc ggccgccagg
     1861 cagatggagc cgcttttgcc caggtggcag cagatgttca tttccagaat gcgccgttct
     1921 cccgtctcga agtcgattct atagtcgatg cggaaatagt ccatcggccc cagagcgctc
     1981 cagattttcc ggacgtcaca ggcgatgtcc tgctccatat cctgaacgga gaccagctga
     2041 taacccagat ggtcgtgcag tttcaggtct tccgtgatga tgctgcctgg tttgtcggat
     2101 ttcggctgca catatcccag gatgacgggg ttttccccac ccagtacggg aacggtgacg
     2161 tcaatgcctt cacagtattg ttccacgagc acttcgtgcc cttcttccat caggcgccgg
     2221 gcgcatgaac ccacggcttt ccaggagctg cagatgctgt cttcccggat gccgccggaa
     2281 gcggctccaa aacgcttttt gacaaaatag gggccaggga agggggcctg ggccggaata
     2341 cctttttctc cggaaagtcc aatgccttcc ggaacgttca attccaggga gcggaatacc
     2401 attttggtca gaaatttgtc ttccgctatg gcgcggatgt tgggcggggc gcccaggtag
     2461 ggaacctgaa tgaattcgca gtaggaagaa atgaatattt caggcctgga catggcgaac
     2521 ctgttcatca gggagaatac atagtccacg tttcccttgg cgaattggac ggaatagggt
     2581 ttggaggagg agacgacgtc atagcctatg tcctggatgg ctttataaat gttgtagtgg
     2641 tactgagggt agcctccatt gcctgtgtag ggttttttgg agaaatcggg agcgtcgggg
     2701 gcatatggag ccaggaacat gagccttgcc ggcttgccat attgattgag ttggatgtct
     2761 ggtatttcaa acatggctta tggctattac gtgtaatgtt tcttggatta tggaatgggc
     2821 cgccatgttc aaataatata aaaaagaagg gacggcatag ccgtccccgg aggtggtggg
     2881 atagaagaag gaaaagcttt tccctatttt ctggacattc ccctggcgac gcgctgaaca
     2941 tattgttcca tggccggaag ttcctgttca atgcattcca ccagtttgag ctgcgtacgg
     3001 cagcggatga gattgtggtt cggccgtgag accttgaagt atgggtcgcc ttccagatag
     3061 tccgttaaga aacggatgcc gatttccagc gtgatgagtt ttcctgaaaa ggcaagctgg
     3121 tctatttccg cctgggtcag gaagccgtgg gcagcggtca gatacccttc tacaatggat
     3181 tggaacatgg gcatgcgcat gcgcaccttg tccaggtctt cttcgtcttc ttccgtaggc
     3241 ggagtgacgg tgcgcaccat atctccgaag tcgtacaaca ccaggccggg catgaccgta
     3301 tccaggtcaa tgacgcagac ggcgtggtcg gtgtcctcat ccagcatgac attgttgatt
     3361 ttggtgtcgt tatgagtgat gcgggtggga agaacacctc tttcctgaag attcagcaac
     3421 cgatccacat cctgttctcg ggctttaatg aattccaatt caggcagaca ggtggagaga
     3481 cgcccctgcg gatccagctc cgccacttcc atcaggcggt tgaagcggtt ccgggtatga
     3541 tggaagccag gtatggtttc cacgatgtcg tccggattca tgtcactaat caaatcctgg
     3601 aaggacccaa aggcaaatcc tgcctggtag gcctggcggg tgttttccac cacgtcgtag
     3661 gtgtgtgtgc cggccaggta gttgtaacag cgccagatgc cgtcaccggg gatgtcaatg
     3721 tagttgcgtc ctccccgggc cgggtagagg ttgagggttt ggccggcgga atccttcagg
     3781 cggcgcagga ctttccagtt gatgtggcgc gtgacttttt ccacgttgcg catgacggct
     3841 ttggggtctt tgaagacgta atcgttaatg cgctggagga tataggaatc ttccgtcccg
     3901 tcgcttttac ggtaggtagc cttgtaagtg gtgttgatgt gtccgctggg tatttccttc
     3961 ccggtgacga attccccttc aatggcgaag aggtctccaa ttccggcgat acggcacaga
     4021 agctggtcgg aagctgtcgt tacggagaga ggggctgtca tgtcaaaggt tgaaagaaag
     4081 ttgaatgaat aagttaacgg atgaatttat aacctacaag ctcatcaagc ctgttcctga
     4141 gccgggaaat accgtctcca ttgagcgcgg aaatggggat gacgtccact ttcgggaaac
     4201 gcatgcggaa gttaaccaga ttttcttccg ctccttccag gtccattttg ttggcgacga
     4261 cgaaccaggg ctgtttggcc aggtcttcgc tgtacagttt aatttccgtg cggagattct
     4321 gaaggtcttc aatggggtcg cggccttcgc tgccggccat gtccagaacg aagacgagca
     4381 ctttgcagcg cgtgatgtgg cgcaggaatt catgtcccag gccccggttg ttgtgtgcgc
     4441 cttcaatgat gccgggaatg tccgccacaa tgcagcggcg aaagctgtcg aattccacca
     4501 ctccaatgat aggctgaagc gtggtaaaag gatagctggc tactttgggc ttggcttccg
     4561 aaatgtctcc cagcaatgtg cttttgcccg cgttggggta gcctaccagc ccggcgtccg
     4621 caatgcggcg caattccata aagaagacgc cttcctcccc ttccgttccc atctcggctt
     4681 ccgtaggcgc ctggttggtc gcggaacgga aatgccagtt tcccttgccg ccgaggccgc
     4741 cctggcacag cgtgaaccgg gtgccgattt ccgtcagatc cgctattttt tccagttcaa
     4801 tgcct
//
LOCUS       DAOMSU010000163         4792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1632_length_4792_cov_2.327845, whole genome shotgun sequence.
ACCESSION   DAOMSU010000163 DAOMSU010000000
VERSION     DAOMSU010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1632_length_4792_cov_2.327845"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4361)
                     /gene="uvrA"
                     /locus_tag="PK077_07425"
     CDS             complement(<1..4361)
                     /gene="uvrA"
                     /locus_tag="PK077_07425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRN24170.1"
                     /translation="MSHCEDGCIRIRGAREHNLQNVDVDIPLGRLTVVTGPSGSGKTS
                     LAMHTLYAEGQRRYMETFSPYVRQFMDRMDKPDVDAVENILPAIALHQRNSVRTSRST
                     VGTMTGLNDYWKFVFARLAVGIDPETGREIKPETPGTIDEKLHAEFPAGTEVMVCLEV
                     ARPESVDLPTLKRNLVAQGYLRAFAHGEILRLEDEDWTLEEGEPLLVVQDRVRLTEDQ
                     RERRLEALETAMRLGGGVAHVIPRVDGVWLSALKFRGDWHPLMEPRPGLFSFNSPLGA
                     CPECRGYGRVITIDYNRCIKPELSVQDGAIHIFEGDGKVFSECKRDLMRGWRKNARKV
                     RLDVPWKDLKQWERDWLLYGDGDDPDEMYEQGLWYGIAGFFKYLESRTHKMHVRVYLS
                     RFRTYQECPSCHGLRLRPEALQFKVGGKSMPELSSMPMDELLAWVDRYVTPRADEDPG
                     LKHAVAELRSRLEYLNEVGLGYLTSDRSTRSLSGGEIERVSLTTCLGASLTDTLFVLD
                     EPTVGLHPRDTSRLISAMNRLKKRGNTLVVVEHEEAVMRAADCLVDMGPGSGREGGRL
                     VYSGMPARIGEIEESLTGAFLSGRRRIAVPKKRRKPRQFLTVSGATRHNLRKLDVKVP
                     LGVFTCLTGVSGSGKSTLAHDVLYLNALVEKGAVCEEEPARVRSIKGWEHLDEVVMVD
                     QSPIVRTPRSTPAVYAGVFEEIRSLFAETETARARGLKPGFFSFNSGDGRCPRCMGMG
                     SEKVEMQFLSDIFVQCPLCHGSRYGSEVLSVYRDGRDIADVLGMTVAAALECFSAEKG
                     AKASRIASKLGVLQRVGLGHLTLGQPLNTLSGGENQRLKLAKILLDQIGSGANSPKML
                     ILDEPGTGLHFADIEVLLAVFRELVEQGHTLLVIEHNPEFIKSADYVIDLGPEGGAGG
                     GHVVATGTPEEIVAAGKGYTGKYLREVLEGNPSVYDPADASVPESADMDIPEGVMALR
                     GARHHNLKNVDLDVPRGEMTVLTGLSGSGKSSLAFDIFFAEGQRRFMDVMSPYARQFT
                     EQLESPDIDRLTGLPPTVAIEQNMSRGGTKSTVGTVTEIWQFMRLLYAKLGQAYCPQC
                     GVPVGKRSESEVVELVARELKKHGGLALLAPLVRGRKGHYADLARWAEGKGYEEMWVD
                     GKLVPLSGFQPLDRYSSHDLDLVVARPEASWPPEKLAGAVHAALEMGEGFLQVLPPGS
                     VRPELMGTRLACASCGQSFPELEPYTFSFNSPRGWCPVCRGHGIVGRGKVKEDQAQSL
                     LEAELKYDRELARQADDDKGITTCPACRGVRLNEFARAVRLQGVTPGDIASLPAVAAA
                     ELVKGWQFEREEALVARDVVAEITQRLNFLQRVGLGYLSLDRSATTLSGGETQRIRLA
                     SQLGSHLRGVLYVLDEPTIGLHPRDNELLLGTLDELKRRGNTLLVVEHDEDTMRRADH
                     IVDMGPGAGIHGGRIMA"
     gene            4578..>4792
                     /locus_tag="PK077_07430"
     CDS             4578..>4792
                     /locus_tag="PK077_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24171.1"
                     /translation="MIKWILTKIVGTKNQREVRRLRPIVEQIVSIEESWNGKGQEFLL
                     EKTREWQGYLHRFLPMDLPPVRIVEAAP"
BASE COUNT          955 a         1577 c         1293 g          967 t
ORIGIN      
        1 tgggccatga ttctgccgcc atgaatgcct gctcccggcc ccatgtccac aatatggtcc
       61 gcgcgcctca tggtatcttc atcatgttcc accaccagca gggtgttgcc ccggcgtttg
      121 agttcatcca aagtgcccag cagcaattcg ttgtcccgcg gatgcagacc gatggtcggt
      181 tcatccagca cgtacagcac accgcggagg tgggaaccca gctgggaagc cagacggatg
      241 cgctgggtct ccccgccgga gagcgtcgtg gcgctccggt ccagggaaag gtagcccagc
      301 cccactcgct ggaggaagtt gaggcgctgc gtgatttccg ccactacgtc acgggcaacg
      361 agcgcttcct cccgttcaaa ttgccaccct ttgaccagtt ctgcggcggc cacggcaggc
      421 agggaggcaa tgtcaccggg agtcacgccc tgaagccgca cggcgcgagc aaattcattc
      481 aaccgcacgc cccggcaggc agggcaggtt gttattccct tgtcatcgtc cgcctgccgc
      541 gccagttccc tgtcatattt cagttcagcc tccagcaggg attgcgcctg atcttccttt
      601 actttccccc tgccgacgat tccatgcccg cggcatacgg ggcaccaccc gcgcggagag
      661 ttgaaggaaa acgtgtacgg ttccagttcc gggaaagatt gcccgcagga ggcgcatgcc
      721 agcctggtgc ccatcagttc cggacggacg gaaccgggcg ggagcacctg caggaaccct
      781 tcccccatct ccagcgcggc atgaaccgcc ccggcaagct tttccggcgg ccaggaggcc
      841 tccggccgtg caacgaccag gtccaggtca tggctggaat agcggtccag cggctgaaat
      901 ccgctcaggg gaaccagttt gccgtccacc cacatttcct catatccctt tccttccgcc
      961 cagcgggcca ggtcggcgta atgccccttg cgtcccctga cgaggggggc cagcaacgcg
     1021 agtccgccat gttttttcag ctccctcgcc accagctcca ccacttccga ttccgacctt
     1081 ttgcccaccg gaacaccgca ctgcgggcaa taggcctgcc ccagcttggc atacagcaga
     1141 cgcatgaatt gccagatttc cgtgacggtt cccacggtgg atttggtgcc cccgcgggac
     1201 atgttctgtt caatagccac cgtggggggc aatccggtca ggcggtcaat gtccggactt
     1261 tccagctgtt ccgtgaattg ccgggcgtac ggggacatga cgtccatgaa acgccgctgc
     1321 ccttccgcga aaaagatatc aaaagccagg gagctcttcc cggagccgga aagcccggtc
     1381 agcacggtca tttctccccg cggcacgtcc aggtccacgt ttttcaggtt gtggtggcgc
     1441 gctccccgca gggccatcac gccttccggt atatccatgt ctgcggattc cgggacggag
     1501 gcgtccgcag ggtcgtaaac ggaagggttg ccttccagca cctccctgag gtatttgccc
     1561 gtatatccct tcccggcggc tacgatttcc tccggcgtgc ccgtggccac cacatggccg
     1621 cctccggcgc cgccctccgg ccccaggtca attacgtagt ccgcggattt gatgaactcc
     1681 gggttgtgtt cgatgaccag cagggtatgc ccctgctcca ccagttcccg gaacacggcc
     1741 agcagaactt caatgtccgc aaagtgaaga cccgttcccg gctcgtccag aatcagcatc
     1801 ttggggctgt tcgcgccgga acctatctgg tccagcagga ttttagccag cttcaggcgc
     1861 tggttttccc cgccggaaag ggtattcaga ggctgcccca gggtcaggtg ccccagccct
     1921 acgcgctgaa gcacgcccaa tttggaagcg atgcgggaag ctttggcccc tttttccgcg
     1981 ctgaagcatt ccagcgcagc cgctaccgtc atgccgagca cgtccgcaat atccctgccg
     2041 tcacggtata cgctcagcac ttcgctcccg taccgggagc cgtggcacag cggacactga
     2101 acgaaaatgt cggaaagaaa ctgcatttcc actttctcac tccccatgcc catgcagcgc
     2161 ggacaccggc cgtccccact gttgaaagag aaaaaaccgg gcttcaagcc acgcgcgcgg
     2221 gccgtctccg tttccgcgaa gagggagcgt atttcttcaa aaactccggc ataaacggca
     2281 ggggtggagc gcggggtgcg gacgatgggg ctctggtcca ccatcaccac ctcatccaga
     2341 tgttcccagc ctttgatgga cctgacgcgg gcgggttcct cctcgcatac ggctcctttc
     2401 tccaccagag cgttcaggta aagaacatcg tgggccaagg tgcttttccc ggacccgctg
     2461 acgcctgtca aacaagtgaa gacgcccagg ggcactttca cgtccagttt gcgcaggtta
     2521 tggcgcgtgg ctccggatac ggtcagaaac tggcgcggct ttcgcctctt ttttggcacc
     2581 gcgatgcgtc gcctgccgga aagaaaagcc cccgtcaggg attcttcaat ttctccaatc
     2641 cgggcaggca tcccggaata aaccagcctt cccccttccc gtccggaacc gggtcccata
     2701 tccaccaggc agtccgcggc acgcatgacc gcttcctcat gctccaccac caccagggtg
     2761 ttcccacgct ttttaagccg gttcatggcg gaaatcaaac gggacgtatc gcggggatgg
     2821 agcccaacgg taggttcatc cagcacaaac agcgtatccg tcagggaagc gcccaggcag
     2881 gtcgtcaggc tcacgcgttc aatttcccct ccggacaggg accgcgtgga gcggtcggac
     2941 gtcaggtagc ccaatcccac ttcattcaga tattccaggc ggctcctgag ttccgccacg
     3001 gcatgtttca agccggggtc ttcatccgcc cgcggcgtca cgtacctatc cacccaggcc
     3061 agcagttcat ccatgggcat actggaaagt tccggcatgc ttttgccgcc caccttgaat
     3121 tgaagggctt ccggcctcag gcgcaaaccg tggcaggagg ggcattcctg atatgtgcgg
     3181 aaacggctca ggtacacccg cacgtgcatc ttatgcgtgc ggctttccag atatttgaaa
     3241 aatcccgcaa tgccgtacca cagcccctgt tcatacattt catccgggtc atcgccgtcg
     3301 ccgtacagca gccagtcccg ctcccattgc ttcaaatcct tccacggcac atccagacgg
     3361 actttgcggg cgtttttgcg ccagccgcgc atcaggtccc gcttgcattc ggaaaatact
     3421 tttccgtctc cctcaaaaat atggatggcc ccgtcctgaa cgctgagttc cggcttgatg
     3481 caccggttgt agtcaatcgt gatgacgcgc ccgtagccgc ggcattccgg gcacgccccc
     3541 agcggggaat taaaagaaaa caggcccggc cgcggttcca tcagcggatg ccagtctccg
     3601 cggaatttca aagcggacaa ccagacgccg tccacacggg gaatcacatg ggccacgccg
     3661 ccgcccaggc gcatggccgt ttccagagcc tccagcctgc gttccctctg gtcttccgtc
     3721 agcctcacac ggtcctgaac caccagcagc ggttctcctt cctccagcgt ccaatcctca
     3781 tcttccagcc ggaggatctc tccatgcgca aaagcgcgca aatacccctg tgccaccaga
     3841 ttccttttca acgtgggaag atccacggat tcgggccgcg ccacctccag gcaaaccatc
     3901 acttccgttc cggcagggaa ttccgcatgc agcttttcat caatggtacc tggcgtttcc
     3961 ggcttgattt cccgccctgt ttccgggtca atgcccacgg ccagacgggc gaaaacaaac
     4021 ttccagtaat cattcagccc cgtcatggtc cccaccgtgg agcgggatgt cctgacggag
     4081 ttgcgctgat gcagggcgat ggccggaaga atattctcta cggcatccac gtccggcttg
     4141 tccatgcggt ccatgaactg gcgcacgtac ggggaaaagg tttccatata acgccgctgg
     4201 ccttccgcat acagcgtgtg catggccaga cttgttttcc ccgatccgga aggacccgtc
     4261 accaccgtca gccgccccag gggaatgtcc acatccacat tctgcaaatt atgctcacgg
     4321 gcgccgcgga tgcggatgca cccatcctca caatgactca tggaaaaaga atatcatcaa
     4381 tttggaatca ttccaagaga agcgcctgtc atgaatccgg gtttccagag gagtccggcc
     4441 aggtccaacc tgcctattac gtcatcatca gcacaaatgg agggccgtgc ttgcatttct
     4501 tccggcagcc gctagtattt ttcgattctt agaccgctga aagcgactct acctcgtcaa
     4561 ttttcaacaa gacaaagatg attaagtgga ttctaaccaa gattgtcggc accaaaaacc
     4621 agcgtgaagt gcgccgtctg cgccccattg tggaacaaat cgtctccatt gaagaatcat
     4681 ggaacggcaa gggacaggaa ttcctgcttg agaaaacgag ggaatggcaa gggtacctcc
     4741 atcgcttcct ccccatggat ctgccccccg tgcgcattgt ggaagcagcg cc
//
LOCUS       DAOMSU010000164         4714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1679_length_4714_cov_2.337197, whole genome shotgun sequence.
ACCESSION   DAOMSU010000164 DAOMSU010000000
VERSION     DAOMSU010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1679_length_4714_cov_2.337197"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="PK077_07435"
     CDS             complement(<1..164)
                     /locus_tag="PK077_07435"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF014699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HRN24172.1"
                     /translation="MRNLVKSLRLTLVFCVFFSICYILVLWIFGLIAGPGGGNAETLV
                     LNGRVVGAANV"
     gene            complement(184..2217)
                     /gene="kdpB"
                     /locus_tag="PK077_07440"
     CDS             complement(184..2217)
                     /gene="kdpB"
                     /locus_tag="PK077_07440"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HRN24173.1"
                     /translation="MKNSNNTSLFQREQVRESLKQSLIKLHPGMMMRNPIMFTVEIAT
                     MIMLLVTVYSLFSDGQGDFLYNFLVFVILFLTLLFANFAEAIAEARGKAQADSLRRTR
                     EETPAKLIVDGTVTVINSSQLKKGDYFECVAGDIIPADGEVVEGLASIDESAITGESA
                     PVIRESGGDKSSVTGGTKVLSDRIKVLVTARPGESFLDKMIALVEGASRQKTPNEIAL
                     TILLAVFTLVFLVVCITLKPAADYSGTEISIAALISLFVCLIPTTIGGLLSAIGIAGM
                     NRALRANVITKSGKAVETAGDISTLLLDKTGTITIGNRKATGFFPVQGFDKQAFVEAC
                     LMSSVSDDTPEGKSIIELGHEYGVHMRNLHTEGARMIQFTAETKCSGINLSDGTEIRK
                     GAFDTIRKIAEEAGNPFPKEAEEIIERVAGNGGTPLVVCINRKVVGVIELQDIIKPGI
                     EERFERLRKMGVKTVMVTGDNPSTARYIAEKAGVDDFIAEAKPEDKLSYIRKEQASGK
                     LVAMMGDGTNDAPALAQADVGVAMNSGTQAAKEAGNMVDLDNDPTKLIEVVEIGKQLL
                     MTRGTLTTFSIANDVAKYFAIVPALFMIAIPELAALNIMHLHSPERAILSAIIFNAII
                     IPILIPLALRGVAYKPIGASSLLLRNILIYGVGGLIAPFIGIKLIDMVVGLFF"
     gene            complement(2302..4011)
                     /gene="kdpA"
                     /locus_tag="PK077_07445"
     CDS             complement(2302..4011)
                     /gene="kdpA"
                     /locus_tag="PK077_07445"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRN24174.1"
                     /translation="MMKTEILGVIAQIVLMVALSYPLGKYIAKVYKKEKTWLDFMKPV
                     ERLMFRLSGINPEEEMNWKQFLRALLTVNLFWFVWGMVLLVSQGYLPLNPDGNVGQTC
                     HQAFNTCISFMVNCNLQHYSGESGLTYFTQLFVIMLFQFITAATGMAAMAGIMNALSK
                     KTTKTIGNFWDYLVLSSTRILLPLSLVVGFILITQGTPMGFDGKMEVTTLEGVEQTIS
                     QGPTAAVVPIKQLGTNGGGYFGVNSSHPLENPTYLSNMVECWSILIIPMAMVLAFGFY
                     LNRKKLGYSIYGVMLFAFLIGVCVNVYQEMNGNPRIEEMGIVQESGAMEGKEIRLGSA
                     ATALWSVVTTVTSNGSVNGMHDSTMPLSGMIQMLNMQINTWFGGVGVGWMNYFAFMII
                     AVFISGLMVGRTPEFLCKKVEAREMKIASIVALLHPFVILVGTSLACYLFVYAPSFVE
                     GEGGWVNNPGFHGLSEFLYEYTSSAANNGSGFEGLADNTWFWNYSCGIVLILSRFIPI
                     IGQVAIAGLLAGKKYIPETSGTLKTDSVTFSAMTFAVIFIVAALSFFPVHALSTIAEH
                     LSL"
     assembly_gap    4406..4415
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1312 a         1249 c         1018 g         1125 t
ORIGIN      
        1 acattggcgg cgcctaccac gcgcccgttc aaaaccaggg tttccgcatt tcctccgcca
       61 gggccggcaa tcaatccgaa tatccacagc accaggatgt agcatatgga gaaaaagacg
      121 caaaagacaa gtgtaagacg caatgatttc actaaatttc tcataacaat atatttattt
      181 cagttaaaaa aacaggccga cgaccatatc aatcagtttg attccgatga agggagcaat
      241 cagaccgccc actccgtaaa tcaggatgtt gcgaagcagc agagagctgg ctccaatggg
      301 cttgtacgcc acgcccctca aggccagagg aatcaggatg gggataatga ttgcattgaa
      361 aataatcgca gacaggatgg ctctttccgg actatgcaga tgcatgatat tcagcgcagc
      421 aagttctgga atcgcaatca tgaacagcgc aggcacaatc gcgaagtact tggcgacgtc
      481 attggcaatg gaaaacgtcg tcaacgtgcc gcgcgtcatc agcagctgct tgcctatttc
      541 tacaacctca atcaatttgg tggggtcatt atcaaggtca accatattgc cggcctcctt
      601 ggcggcctgg gttccgctgt tcatggctac gccgacatcg gcctgcgcca gagccggggc
      661 gtcattcgtc ccgtccccca tcatggcaac cagtttgccg gaagcctgct ccttcctgat
      721 ataactcaac ttatcttcgg gcttggcttc cgcgatgaaa tcatcaacgc ctgctttttc
      781 agcgatatac ctggccgtac tcggattatc ccccgtcacc attaccgtct ttactcccat
      841 cttgcgcagg cgttcgaagc gctcttctat gcccggcttg atgatatcct gcaattcaat
      901 aacgccgaca accttcctgt tgatgcagac gacaagaggg gtgccgccat tgcccgccac
      961 tctttcaatg atttcctcgg cttctttggg aaatggattt cccgcttcct cggcaatctt
     1021 gcggatggtg tcaaaagccc ccttccggat ttcggttcca tcgctcagat taatgcccga
     1081 acacttcgtc tccgccgtga actgaatcat gcgggccccc tccgtatgaa ggttgcgcat
     1141 atggacgccg tattcatgtc caagctcgat aatcgatttc ccttccggag tatcgtctga
     1201 aacggaagac atcagacagg cttcaacaaa agcctgcttg tcaaaaccct ggacagggaa
     1261 aaatcccgtc gctttccgat tgccaatcgt aatcgtaccc gttttatcaa gcaggagggt
     1321 gctgatgtcc ccggccgtct ccacagcctt ccctgacttc gtaataacat tggccctcaa
     1381 ggcacgattc attccggcaa tgccgatcgc agaaagcaat ccgccgatag tcgtaggaat
     1441 gagacatacg aacagggaaa tcagggcggc aatgctgatt tccgttccgg aataatcggc
     1501 tgccggcttc aaggtaatgc agacgacaag gaaaaccagg gtaaagacag ccaacaaaat
     1561 ggttaacgct atctcgttag gggttttctg ccgggaagcg ccttcaacca gagcaatcat
     1621 cttatcaagg aaactctctc cgggacgcgc ggtgacaagc accttgatac ggtctgacag
     1681 aactttggtg ccgcccgtca cggaactctt atccccgccg gactcacgga taaccggagc
     1741 ggattccccc gtaatagcgc tttcgtcaat ggaagccagc ccttcgacaa cctccccgtc
     1801 ggcgggaatg atatctcccg cgacacattc gaaataatcc ccttttttca actgggaact
     1861 attgatgacg gtaaccgtgc catcgacaat caacttggcc ggcgtttcct cacgcgtcct
     1921 gcgcaggctg tcggcctgcg cctttccccg ggcctccgca atcgcctcgg caaaattggc
     1981 gaacagaagg gtgaggaaca aaatgacgaa aacgaggaaa ttataaagga aatccccttg
     2041 tccgtcgctg aagagggaat aaacggttac cagaagcatg atcatcgtgg cgatttccac
     2101 ggtaaacatg atcgggttcc tcatcatcat tcccggatga agcttgatca atgactgctt
     2161 caagctttcc cgtacctgtt ccctttggaa cagggaagta ttattggaat ttttcatatc
     2221 gtagatttct ttttcaattc cagaaactgg aacggaaata tgtaaatatg ttattggtgg
     2281 taaacgtatt ctaccctaaa tttacaaact cagatgttcg gcaatggtgc ttaaggcatg
     2341 gacagggaag aaggacaaag ccgcaacaat gaaaatcacg gcaaaagtca tggcgctgaa
     2401 cgttacggaa tccgtcttca acgttccgga cgtctcggga atatacttct tgcccgccag
     2461 aagcccggcg atggcaacct ggccgatgat cgggatgaaa cggctcagaa tgaggacaat
     2521 gccgcatgag taattccaga accaggtatt atcggccagc ccttcaaacc cggaaccgtt
     2581 attggcagca cttgaagtat actcgtaaag gaattcactc aatccatgaa agccgggatt
     2641 attgacccac cctccttcgc cttccacaaa cgacggcgcg taaacaaaca gataacaagc
     2701 cagagacgtt ccgacgagaa tcacaaacgg gtggagcaac gccacaatgg aagcgatttt
     2761 catttcacgg gcttctactt tcttgcacag gaattcgggg gttcttccca ccatcagacc
     2821 gctgatgaac acggctataa tcatgaaggc gaaataattc atccacccta ccccgacgcc
     2881 gccaaaccac gtattaatct gcatgttcaa catctgaatc atgcctgaaa gaggcatggt
     2941 ggaatcatgc atcccgttga cggacccatt tgacgtaact gtcgtaacga cgctccacaa
     3001 agctgtagcc gcggatccga gccggatttc cttaccttcc atagccccgc tttcctggac
     3061 gatgcccatc tcttctatgc ggggattgcc gttcatctcc tgatacacgt taacgcagac
     3121 gccaattaaa aaggcaaaca gcattacgcc gtaaatactg tatcccagtt tcttgcgatt
     3181 tagataaaaa ccaaaggcca gaaccatcgc catcggaata atcaggatgg accagcattc
     3241 aaccatgttg gagaggtagg tcggattttc cagcggatgg gatgaattaa cgccgaaata
     3301 acccccacca ttagttccca actgtttaat gggtacgaca gccgcagtag ggccttggga
     3361 aatggtttgt tccactccct caagggttgt cacttccatt ttaccgtcaa accccatcgg
     3421 cgtaccctga gtaatcagga tgaatccgac caccagagaa agaggcagca aaatacgggt
     3481 agaactcaac acgaggtaat cccagaagtt tccaatcgtt ttagttgtct tcttactgag
     3541 agcgttcatg atccctgcca ttgcggccat gccggtagct gccgtaataa attggaacag
     3601 cataatcacg aacaactgcg taaaataggt caaaccgctc tccccgctgt aatgctggag
     3661 gttgcaattc accatgaatg aaatgcatgt attgaacgcc tggtggcacg tctgtccgac
     3721 gttgccgtcc ggattgaggg gcaggtaccc ctggctgacc agaagaacca tcccccatac
     3781 gaaccagaac aaatttacgg tcaataaagc ccgcaggaac tgcttccagt tcatttcttc
     3841 ctccggatta atcccggaca ggcggaacat cagcctttca acaggcttca tgaaatcaag
     3901 ccatgttttc tctttcttgt aaaccttggc aatatatttc cccagaggat agctcaaagc
     3961 caccataaga acgatttgcg caataacacc tagaatttct gttttcatca tcttgctggt
     4021 taaaattttt ccggtttgat caagacatac atcaaataac cgaacacaat taagctaatc
     4081 acaaataata atgtatacat agacaggcac agttttagat tttttcaaac cagtcgacgc
     4141 atttgaaaaa gagccaaaaa cagaatattc cgctcaatag acaaaagata aaacccaaca
     4201 atgattccat catgatattt tatgaaattt aaattcaaac cattccaaca tatatggaaa
     4261 caaaaatatt attctatgcc gtttttcttt tttatctgaa aaaatttcac ggaaaaaccg
     4321 ctatggcaga tacgaagtcc cggaacggac cggcgatgtt taatataatg taaaaacatt
     4381 acctcaatat atccggaaat tccatnnnnn nnnnngggta gttaacttcc ttcttcttct
     4441 tgttaagaaa aaattaattc ttccggctgc gctcccgctt tccgttcttc ccggaaggcg
     4501 gataacggca agtctttctc aacccgttta cacaaaaact gcacccggaa cataccggat
     4561 gcagcttgaa tcccatacag aattctaaaa ggcttcctca acaaaattta cagaactcac
     4621 tctgcatgaa tacctatctc tttccaatcc ccgttgacaa atttccaaca ggaatgaggc
     4681 cgctccaaag atatcaaacg aattgatacc gatg
//
LOCUS       DAOMSU010000165         4704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1688_length_4704_cov_2.650893, whole genome shotgun sequence.
ACCESSION   DAOMSU010000165 DAOMSU010000000
VERSION     DAOMSU010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1688_length_4704_cov_2.650893"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="PK077_07450"
     CDS             complement(<1..749)
                     /locus_tag="PK077_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRN24175.1"
                     /translation="MSPVLTIFRKELRSYVMTPYGWVILAFVMALQSVSLSGTLKAFQ
                     LAPQKEGILFFILHSPMFWFYFLFIFPLLTMRSLAEEEKTGTLESLLTTPIKTWQVVL
                     GKYFSAYAFYIILWLPMLLYPLLADWSNLIVQWIYGYDAGMVLPYRLADWAGAYAILL
                     LIGAWFTAIGIFASSMTGSQIISGIITIGLLVLIFFMGLIPVVWGEFPAAGIFHYMSC
                     SEHLDRFSAGLVDTRPVIFYLTMTVLTLAVT"
     gene            complement(803..1738)
                     /locus_tag="PK077_07455"
     CDS             complement(803..1738)
                     /locus_tag="PK077_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN24176.1"
                     /translation="MIHADQLSKEFGRFQAVKNASFQIEKGEIVGFLGPNGAGKTTTL
                     RMLTGYLPPTSGTATIAGFDIVKNPLEARKHLGYLPEHVPLYDDQRVTEYLKFRARLK
                     GISSKQIWPAVSKVVEQCGLDPVRRKMIRTLSKGYRQRVGLADALLGEPDLLILDEPT
                     NGLDPNQIRQIRELIKGLAANHTIILSTHILSEVEMICNKVIIIDQGTIKAADTPSNL
                     TANLRAAGKITLEFRGDLALITQKLENLEHVKKVIHEGTDADGWHTLTVRAEAGTDTR
                     EKAARLLAEQGCPLRHIYRHTPTLEEVFVEMTRKD"
     gene            complement(1897..3303)
                     /gene="dnaA"
                     /locus_tag="PK077_07460"
     CDS             complement(1897..3303)
                     /gene="dnaA"
                     /locus_tag="PK077_07460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRN24177.1"
                     /translation="MTPPSELSATWSRITGALQTIMSPEVYGLWFPKFSLLEDSGKAL
                     TLVCDDPMAALWVENSYTPELKQAAMLALGTERQIKFICTDEVASMPAAEGQPQKASS
                     RAKTPQTADSPAAPAKKQRTPAARACLNDLYTFDSFVVYEDSRFAYQAGLSAAQSERA
                     LFNPLFLYGKSGVGKTHLLQAIGHEVLHQDSSTNVVYVTGEQFANEFIDASRTQNGQS
                     FTKLRRKYRKADVLLVDDVQFISGKEKTVEEFLHTFDELFHAHKTIVICADAAACDIS
                     NLDPRLAARLESGLTVELNLPDDNARLEILRSKRDRAGMNVSDEILEFLASRIQKSVR
                     RLEGALLRVATFTSLSGDMPDIAKIEQLLRDILREETSRILTVDSIQKRVADFYELKV
                     SDLTGKRRPNSIAFPRQIAMYLSRRLTERSLKDIGQAFGGRDHGTVIHANKLVASRME
                     EDVRVRDIVQRLEEELRD"
     gene            3691..3819
                     /locus_tag="PK077_07465"
     CDS             3691..3819
                     /locus_tag="PK077_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24178.1"
                     /translation="MGDEKDMITPPEINHLQEPDWNCTGRCRREAFRMVDNRRTET"
     gene            3833..4390
                     /locus_tag="PK077_07470"
     CDS             3833..4390
                     /locus_tag="PK077_07470"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRN24179.1"
                     /translation="MEQMEQPEEACLSLCRGKFLELLKEGRWEYVRRVNASGAVMIVA
                     VTEEGELLLVEEYRVPLHARTVGLPAGISGDEGEESSLQSARRELEEETGYRADSWTY
                     LLAGPSSPGLTTEMVSFYLAEGLHRISEGGGVAHENITVHRIPLTLVHDWLMEQMEQG
                     KVVDPKIFMGLYFLSRSVNGMGLEE"
     gene            4605..4679
                     /locus_tag="PK077_07475"
     tRNA            4605..4679
                     /locus_tag="PK077_07475"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4637..4639,aa:Met,seq:cat)
BASE COUNT         1053 a         1194 c         1400 g         1057 t
ORIGIN      
        1 atggtgacgg ccagcgtcag aaccgtcatg gtcaggtaaa atatgacggg gcgcgtgtca
       61 accagcccgg cggaaaagcg gtccagatgt tccgagcagg acatgtagtg gaaaattccc
      121 gctgcgggaa attcccccca caccacggga atcagcccca tgaagaaaat cagaaccagc
      181 aggccgatgg tgatgatgcc ggaaataatc tggctgcccg tcatggaaga ggcgaaaatg
      241 ccaatggccg tgaaccaggc gccgatcagc agcagaatag cgtatgcccc ggcccagtcc
      301 gcaaggcggt agggaagaac catgcccgca tcatacccgt aaatccactg tacaatcagg
      361 ttggaccagt ccgccaggag ggggtatagc agcatgggca gccacaagat gatgtaaaaa
      421 gcgtacgcgg agaagtattt tcccaggacc acctgccatg tcttgatggg cgtggtaagc
      481 agggattcca gcgttcccgt cttttcctcc tccgccaggg aacgcatggt gagcagaggg
      541 aagatgaaca ggaagtaaaa ccagaacatg ggggaatgca ggatgaagaa caggatgcct
      601 tccttctgcg gggccagttg aaaagccttc agcgtgccgg acagggagac gctctgaagg
      661 gccatgacga aagccaggat aacccatccg tacggagtca ttacgtagct tctgagttct
      721 tttctgaaaa tggtaagtac tggagacatg gggagaaaag ggaaatggga ggtggtggat
      781 gggcggggat ggaaaatggg ggttaatctt tacgggtcat ttccacgaac acttcttcca
      841 gcgtgggggt gtggcggtaa atatggcgca gggggcatcc ctgttccgca aggaggcgcg
      901 cggccttttc acgggtatcc gtcccggcct ccgcgcgtac ggtcagcgta tgccagccgt
      961 cggcgtccgt tccttcatgg atgaccttct tcacatgttc cagattttcc agtttctgcg
     1021 taatcagggc caggtctccc cggaattcca gcgtaatctt gccggcggcc cggagattgg
     1081 ccgtcaggtt ggagggggta tccgccgcct tgatagttcc ctggtcaatg atgatgacct
     1141 tgttgcaaat catttccacc tcactgagaa tgtgggtgga aagaataatg gtgtggttgg
     1201 ccgccagacc tttgattagc tctcgtatct ggcgtatctg gttggggtcg agaccgttgg
     1261 taggttcgtc aaggatcagc aggtccggtt cccccagcag ggcgtccgcc aggcctacgc
     1321 gctggcggta gcctttggaa agggtgcgaa tcatcttgcg gcgcacggga tccaggccgc
     1381 attgttctac aaccttggag acggcaggcc agatctgctt ggaagagatg cctttaagcc
     1441 tggcgcggaa tttcaggtat tccgtgacgc gctggtcgtc gtaaaggggc acgtgttccg
     1501 gcaggtagcc cagatgtttg cgagcctcca ggggattttt cacaatgtca aaccccgcaa
     1561 tcgtggctgt gccggaagtg gggggcagat agccggtcag catgcgcaaa gtcgtggtct
     1621 tgcctgcgcc gttcggcccc aggaacccta cgatttcccc cttttcaatc tggaaggagg
     1681 cattctttac cgcttgaaag cggccaaact ctttggaaag ctggtcggcg tggatcatgg
     1741 aatcaatggg aacagggtga ttctaatcca aaccgctttc gcctcaagct tattctcccc
     1801 tgcgccgcgg aagcgcttct tccacctgaa aagccatggg ggctaacggg accgatgctt
     1861 ttcctgtctg gccgggaggg aatgaagata aggaaatcaa tccctcaact cttcttccag
     1921 cctctggacg atgtccctca cgcgtacatc ttcttccata cgggaggcga ccagcttatt
     1981 cgcatggatg acggtgccgt ggtcccggcc gccgaatgcc tggccgatat ctttcaggga
     2041 gcgttccgtc aggcggcggc tcaagtacat ggcgatctgg cgcggaaagg cgatgctgtt
     2101 ggggcggcgc ttgccggtca gatcgctgac cttgagttcg taaaaatccg ccacgcgctt
     2161 ctggatggaa tccacggtca ggatgcggct tgtttcttcc cgcaggatgt cgcgtagaag
     2221 ctgctcaatt ttggcgatgt caggcatgtc tccggacagg gaagtaaagg tggctacacg
     2281 gagcagtgcg ccttccagac ggcgcacgct cttctggatg cggctggcca gaaactccag
     2341 aatttcgtcg gatacgttca tgccagcccg gtcgcgcttg ctgcgcagga tttccagccg
     2401 tgcgttgtcg tccggaaggt tcaattctac cgtaagtccg gattccaggc gtgccgccag
     2461 ccggggatcc agattggaaa tatcgcatgc ggcggcgtct gcgcaaatca cgatggtttt
     2521 gtgggcatgg aacagttcgt caaacgtatg cagaaattcc tccaccgttt tttccttgcc
     2581 ggaaataaac tgcacgtcgt ccaccagcag gacgtcagcc ttgcggtact tgcggcgcag
     2641 tttggtgaag ctttgtccgt tctgggtccg ggaagcgtca ataaattcat tggcgaactg
     2701 ttcgcccgtg acgtacacca cattggtgga tgaatcctga tgaagcacct catggccgat
     2761 ggcctggagc agatgggttt tgccaacgcc ggattttccg tacaggaaaa gcggattgaa
     2821 cagggcccgt tccgactgtg cggcggacag ccctgcctga taagcaaagc ggctgtcttc
     2881 ataaaccaca aacgaatcga acgtatataa gtcattcagg catgccctgg cggccggtgt
     2941 gcgctgtttt tttgccggtg cggccgggga atccgccgtc tgcggcgttt tggcccggga
     3001 ggaagccttt tgcggctggc cttctgcagc gggcatggaa gccacttcat ccgtgcagat
     3061 gaactttatc tggcgttccg tgcccagagc cagcatggcg gcctgcttca gctccggcgt
     3121 atagctgttt tccacccaga gggcggccat aggatcgtcg cacaccagcg tcagcgcctt
     3181 ccctgaatcc tccagcaggg aaaacttggg aaaccacaat ccataaacct ccggggacat
     3241 gattgtttgc aaggctcccg tgattctgga ccaggtggct gacagttctg atggcggcgt
     3301 cattgttatt ggaaataaaa gatgttgctg gttagaaata gccgtaaggc gttccgtccc
     3361 gctacgaggc cgcccgcgga tggaaccgga tcaggttcat ccgcagccgt gacagctcgt
     3421 ggcgtccatc ggggtggaga aggcgaactc atccgctttc agatcaacac cccggttgac
     3481 gggagcgcca aattactgat ttgcgactga gaaaaaaata tctttttgaa acagcgcgag
     3541 tcatcgttcc tcggccctac gttccacggg ggttccacat gaaaaaatat tttgttcttg
     3601 cttcctccga taatttagtc ctcctgagta tattttctgt gtaattttat aaattattta
     3661 ttgtcaatat attatggttt tgttgcaggc ttgggtgacg aaaaggacat gattacgccg
     3721 ccggaaatca accacttaca ggaaccggat tggaactgca cgggaagatg cagaagggag
     3781 gcgttccgca tggttgacaa cagacggacg gaaacctaac gtgggctgca tcatggaaca
     3841 aatggaacag ccagaggaag catgcctgtc tctctgccgg gggaaatttc tggaactcct
     3901 gaaagaggga aggtgggaat acgtgcgccg ggtgaatgcc agcggagctg ttatgatcgt
     3961 ggccgtgacg gaagaaggag aactgcttct ggtggaagaa taccgtgttc ccctgcatgc
     4021 ccggaccgta gggctgcctg cgggtatttc cggggatgaa ggggaggaaa gctcgctgca
     4081 gtccgcccgg cgtgaactgg aagaggaaac cgggtaccgg gcggattcct ggacgtatct
     4141 tcttgcgggg ccttcctctc cgggattgac tacggaaatg gtatctttct atctggcgga
     4201 aggcctgcat cgaatatcgg aaggaggcgg tgtggcccac gaaaatatta ctgtgcaccg
     4261 cattcctctt actctggtgc atgactggct gatggagcag atggagcagg gaaaagtggt
     4321 ggatccgaaa atctttatgg gtctctattt cctgtcccgc tctgtcaatg gcatggggct
     4381 ggaggaataa tcataccctg cgttgtaaat gtcggtatgc aggaaatggt ccgggacgcg
     4441 ggccatatga gacggttgag tggaaaaatg attcatcagt aatgatttcc ccggagggaa
     4501 aggcggattg tcatgataaa atgctcctga accataaaaa aatctggaca ttctttccaa
     4561 tccgggctaa atatctcccc gccttacggc atcaaccact taacggcagg gtagctcagt
     4621 ggtagagcag aggactcata agcctttggt cgggtgttca aatcacctcc ctgctaccat
     4681 cttcaaagcc ccttaaatcc aacg
//
LOCUS       DAOMSU010000166         4676 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1705_length_4676_cov_2.939840, whole genome shotgun sequence.
ACCESSION   DAOMSU010000166 DAOMSU010000000
VERSION     DAOMSU010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4676)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1705_length_4676_cov_2.939840"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1020)
                     /locus_tag="PK077_07480"
     CDS             complement(<1..1020)
                     /locus_tag="PK077_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HRN24180.1"
                     /translation="MTSPPDNQDTPPAKASLWALSPLLVFFLLYLVISIAVQDFYAVP
                     VTVAFMAAAVWAVFITRGKSMAERVDLFSSGVANRNILMMVWIFVLAGAFAQSARDMG
                     AIDATVQLTLRLLPDNLLFASVFIASCFISLSVGTSVGTIVALAPVATGLAQATQVSA
                     GMMTAIVVGGAFFGDNLSFISDTTIAATRTQGCAMRDKFRANIKVALPAALLALACYI
                     LLGWNVQSPSTADISIEWMKVFPYLLVLVTALAGIHVMAVLTMGLLATGLIGVGMGYF
                     SFMAWFQAMGSGMTGMGELIIVTLLAGGVLSIIRFNGGIAYIIGKITAHIRSRRGAEF
                     SIAGLV"
     gene            complement(1047..>2131)
                     /locus_tag="PK077_07485"
     CDS             complement(1047..>2131)
                     /locus_tag="PK077_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRN24181.1"
                     /translation="KNSRIFVAGHRGLVGSAIWKSLENKGYSYLIGRTHKELDLEDPA
                     AVREFFDREKPEYVFLAAAFVGGIIANSRYRADFIFRNLQIQQNVIGESFRHGVSKLL
                     FLGSTCIYPREAPQPMKENALLTSPLEYTNEPYAIAKIAGLKMCESFNLQYGTNYIAV
                     MPTNLYGPNDNFHLENSHVLPAMVRKIHLAKCLMEGDWNAVRKDLDMRPVEQVDGTAE
                     EREILAVLDKYGITPGSVELWGTGTPLREFLWSEDMADACVHVMEQVDFSQLQEGGRE
                     VRNCHINIGTGKEISIGGLARLIAATAGYRGNLAFNADKPDGTMRKLTDVSKLHSLGW
                     KHRVELEEGVERIYRWYLSRMEADGN"
     assembly_gap    2132..2165
                     /estimated_length=34
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2166..3227)
                     /gene="gmd"
                     /locus_tag="PK077_07490"
     CDS             complement(<2166..3227)
                     /gene="gmd"
                     /locus_tag="PK077_07490"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRN24182.1"
                     /translation="MSKIALISGITGQDGSFLAEFLIDKGYEVHGILRRSSSFNTGRI
                     EHLYLDEWVRDMKKNRLINLHYGDMTDSSSLIRIIQTVQPDEIYNLAAQSHVKVSFDV
                     PEYTAETDAVGTLRMLEAVRILGMEKKCRIYQASTSELFGLVQEVPQKETTPFYPRSP
                     YGVAKQYGFWITKNYRESYNMFAVNGILFNHESERRGENFVTRKITLAAARIAQGMQD
                     KLYLGNLNALRDWGYAKDYVECMWLILQHDTPEDFVIATGEMHTVREFCTLAFREAGI
                     ELEWEGEGVEERGRDRKTGNILVEVDPKYFRPAEVEQLLGDPTKAKTLLGWNPTSTPF
                     EELVRIMVRHDMKYVKTPVH"
     gene            complement(3383..4501)
                     /gene="carA"
                     /locus_tag="PK077_07495"
     CDS             complement(3383..4501)
                     /gene="carA"
                     /locus_tag="PK077_07495"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HRN24183.1"
                     /translation="MRAILALEDGSVFIGSHFGATGTEVGEACFNTSMTGYQEVLTDP
                     SYSGQIVTMTYPLIGNYGVNPEDGESGKAQVSGFVVAELAKVHSNWRATESLGPWLEK
                     QGVIGIEGVDTRKIAKHLRSAGAMRACLTTELTPEEAVEAARNAPSMEGCNIVDKIGS
                     HPMTPEEVDALVTGKTLPPAEYDIVAFDFGIKYNILRQLRENGFRVTVVPAHTTAEEV
                     LAMNPDGVFLSNGPGDPATLTDIHREVAALIGKVPMFGICLGHQIISHALGAKTFKLK
                     FGHRGANHPVKDLKTGKISITSQNHGFAVDPDTVPDNVEITLINLNDNTVEGIAHRTL
                     PVFSVQYHPEAAPGPRDPQYLFRDFRKMIAASKRQHAR"
BASE COUNT          911 a         1267 c         1383 g         1081 t
ORIGIN      
        1 gaccaatccg gcaatgctga attccgcgcc gcgcctgctg cggatatggg ccgtaatttt
       61 tccgataata tacgcgatgc ctccgttgaa acggatgatg gacagtacgc cgccggccag
      121 cagagtgacg atgatcagtt ctcccatgcc ggtcatgccg ctgcccatgg cttggaacca
      181 ggccatgaag gagaaatacc ccatgccgac gccaatcagt cccgttgcaa ggagccccat
      241 ggtcagaacg gccatgacgt ggattccggc cagggccgtc accagcacga gcagataggg
      301 aaatactttc atccattcaa tggagatatc cgctgtggag ggggactgca cgttccatcc
      361 gagcaggatg tagcaggcca gggccagcag agcggcgggg agcgccacct ttatgttggc
      421 ccggaatttg tcccgcatgg cgcagccctg cgtacgggtg gcggcgatcg tggtgtctga
      481 aatgaaggaa agattgtctc cgaagaatgc tccccccacc acaatggccg tcatcatgcc
      541 ggcactgacc tgcgtggctt gtgccagccc tgtggctacg ggagccagag cgacaatggt
      601 gcctacggat gttcctacgg agagggagat gaaacaggag gcgatgaaca cgctggcaaa
      661 cagcagattg tccggaagaa gccgcagcgt aagctgcacg gtggcgtcaa tggctcccat
      721 atccctggca gactgggcga aggctcctgc cagcacgaaa atccagacca tcatcaggat
      781 gttccggttg gctactccgg aggaaaacag atccacccgt tccgccatgc tttttccgcg
      841 ggtgatgaaa acggcccaga cagctgccgc cataaaggct accgtgaccg gcacggcgta
      901 aaaatcctgt acggctatgg agatgaccag gtaaagcagg aagaaaacca gcagggggct
      961 cagtgcccac aggctggctt tggcgggggg cgtatcctgg ttgtccgggg ggctggtcat
     1021 tggagtgagg ggagctggcc gaaaagtcag ttgccgtcgg cttccatccg gctcagatac
     1081 caccggtaaa tgcgttccac tccctcttcc agctccaccc ggtgtttcca gcccagggag
     1141 tgcagcttgg agacgtccgt cagcttgcgc atggtgccgt ccggcttgtc tgcgttgaac
     1201 gccagatttc cccggtaccc tgccgtagcg gcaatcaggc gggccagtcc gccgatggag
     1261 atttctttcc cggttccgat attgatgtgg cagttccgca cttcccttcc tccttcttgc
     1321 agctggctga aatccacttg ctccatcaca tggacgcagg cgtccgccat gtcttcgctc
     1381 cacaggaatt cgcgcagggg agttcccgta ccccacagtt ccacggaacc gggggtgatg
     1441 ccgtatttgt ccagcacggc caggatttcc ctttcttcag ccgttccgtc cacctgttcc
     1501 acagggcgca tatccagatc tttccgcacg gcgttccagt ctccttccat caggcacttg
     1561 gccaggtgta tcttgcgtac catggcgggc agcacgtggc tgttttccaa gtggaaattg
     1621 tcattggggc cgtacagatt tgtaggcatg acggcgatgt agttggtacc gtattgcagg
     1681 ttgaagcttt cgcacatttt cagccctgct attttggcaa tggcataggg ttcgttggtg
     1741 tattccagcg gggaggtaag cagggcgttt tccttcatgg gctggggcgc ttcgcgcggg
     1801 tagatgcagg tgctccccag gaagaggagc ttggatacgc catgccggaa gctttccccg
     1861 atgacgttct gctgaatctg gagattgcgg aagatgaagt ccgcccggta gcgggagttg
     1921 gcgatgatgc cgcccacaaa cgccgctgcc agaaagacgt attccggctt ttcccggtca
     1981 aaaaattccc ggacggccgc agggtcttcc agatccagtt ctttgtgcgt ccggccaatc
     2041 aggtaggaat agcctttgtt ttccagggat ttccagatgg cggaaccgac gaggccgcgg
     2101 tgtccggcca caaagattct gctgtttttt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnatgaa cgggagtttt tacgtatttc atgtcgtggc gcaccatgat gcgcacgagt
     2221 tcctcaaacg gagtggaggt agggttccag ccaagaaggg ttttggcctt ggttgggtct
     2281 cccaacagtt gttcaacttc ggcagggcgg aaatatttgg ggtccacttc caccagaata
     2341 ttgcccgtct tcctgtcgcg tccgcgttct tccacgcctt ctccctccca ttccagctct
     2401 attccggcct cacggaaggc cagcgtgcag aattccctga ccgtgtgcat ttcccctgtg
     2461 gcgatgacga aatcctccgg tgtgtcatgc tggagaatca gccacatgca ttccacgtaa
     2521 tccttggcgt aaccccagtc gcgcagggcg ttcaggttgc ccaggtaaag cttgtcctgc
     2581 atgccctggg caatgcgtgc cgccgccagc gtgattttgc gggtaacgaa attttctccg
     2641 cggcgttcgc tttcgtggtt gaacaggatg ccgttaacgg caaacatatt gtaagattcc
     2701 cggtaattct tggtaatcca gaatccgtat tgcttggcta cgccgtaggg tgaacggggg
     2761 taaaacggcg tggtttcctt ctgggggact tcctgcacca gcccgaaaag ctcggacgtg
     2821 gaagcctggt agatgcggca tttcttttcc atgcccagaa tgcggacggc ttccagcatg
     2881 cggagggttc ccacggcatc cgtttccgcc gtgtattccg gtacgtcaaa gctgactttc
     2941 acatggctct gggctgccag attgtagatt tcatccggct gcacggtctg gatgatgcgg
     3001 atgagagaac tggaatccgt catgtccccg tagtggaggt taatcaggcg gtttttcttc
     3061 atgtcccgca cccattcgtc caggtaaagg tgttcgatgc ggcccgtgtt gaaggaggag
     3121 gaccggcgca ggatgccgtg tacttcatac cctttgtcaa tcaggaactc tgcgagaaag
     3181 gaaccgtcct gtcccgtaat gccggagatt aatgcgattt tgctcatatg gtgtgtcagg
     3241 ttatttgctg gcgggagagg ttgatgaaat gaaaagaaac ctctcccgga gccgcaattc
     3301 atgggatccg ggcataaata acgcattttt cccagcgagg cgaggaaaat gctctggtct
     3361 ataggaaggg ggggcagagc cgtcagcggg catgttgccg cttgctggcc gcaatcattt
     3421 tcctgaaatc ccggaacaga tactggggat ccctggggcc gggtgccgct tccgggtggt
     3481 attgcacgct gaatacggga agcgtcctgt gggcgatgcc ttccacggta ttgtcattca
     3541 gattgatcag cgttatttcc acgttgtccg gcacggtgtc cggatccacg gcaaaaccat
     3601 ggttctggga cgtaatgctg attttgcctg ttttcaaatc tttgaccgga tggttcgctc
     3661 cacggtgccc gaacttcagc ttgaaggttt tcgctcccag cgcgtgggaa ataatctggt
     3721 ggcccaggca gatgccgaac atgggcactt tcccaatcaa tgcagctact tcccggtgaa
     3781 tgtccgtcag cgtggcggga tctcccggac cattggaaag aaagacgccg tccgggttca
     3841 tggcaagcac ttcctccgcc gtcgtgtggg cgggaactac ggttacgcgg aagccgtttt
     3901 ccctcaactg gcgcaggatg ttgtatttaa tgccgaagtc aaaggcgacg atgtcgtatt
     3961 ccgcaggggg cagagttttt cccgtaacca gcgcgtccac ttcctccggt gtcatggggt
     4021 gggaaccgat tttgtccacg atattgcagc cttccatgga gggggcgttt ctggctgctt
     4081 ccacggcttc ctccggcgtc agctccgtgg tgaggcaggc gcgcatggct ccggcggagc
     4141 gcagatgctt ggcgattttg cgggtatcca ccccttcaat gccgatgacg ccctgttttt
     4201 ccagccacgg gcccagggat tccgtggcac gccagttgga gtggactttc gccagttccg
     4261 ccaccacaaa accgctgacc tgcgctttgc ctgattcccc gtcttccggg ttgacgccgt
     4321 aattgccgat cagaggataa gtcatggtga cgatctggcc gctgtaggac ggatccgtca
     4381 atacttcctg atagccggtc atggacgtgt tgaagcaggc ttcccccact tccgtgcccg
     4441 tggccccgaa gtgcgaaccg atgaagacgc ttccgtcttc cagtgcgaga atggctctca
     4501 tggtgatgta agtatgctgt aggtggtgcg ggattaaagg ttttgggcgt ggtattcctg
     4561 aatggtgcac acctgaagat ttttgttctt cagcgcctca atggcgttca cgcaggcctg
     4621 cgcggaagcc aggttggtgc aataggaggt tttggcggca atggcggagg aacgga
//
LOCUS       DAOMSU010000167         4643 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1728_length_4643_cov_2.323017, whole genome shotgun sequence.
ACCESSION   DAOMSU010000167 DAOMSU010000000
VERSION     DAOMSU010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1728_length_4643_cov_2.323017"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..538)
                     /locus_tag="PK077_07500"
     CDS             complement(35..538)
                     /locus_tag="PK077_07500"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRN24184.1"
                     /translation="MMNRHFIFALCTVLGFSVSGISMAQSVPTDKEAAAKAAKAAKVK
                     FRWGKSLKSAQKQAAETGLPIVLLFTGTSWCGFCVKLEKEILSKKDFKQGMDGVAIGV
                     KFEFGSSDFSKSKEAKTYNITGVPAMVVVDADGKELGRTGYVPGRTPAQYVEFFKKYA
                     PKTAEGK"
     gene            712..786
                     /locus_tag="PK077_07505"
     tRNA            712..786
                     /locus_tag="PK077_07505"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Val,seq:gac)
     assembly_gap    1133..1142
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1212..2090)
                     /locus_tag="PK077_07510"
     CDS             complement(1212..2090)
                     /locus_tag="PK077_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24185.1"
                     /translation="MIDSIDNSMAAPFRDAEGSGFVSPETDALSETAVQTEPFPPLLG
                     EDGGFAPDWYARFDELKGMEKSLSKFKTPEALAKSYAELERLRRYPGVENEEQMARFR
                     RLAGLPESEEEYRLERPESTPESEWNADLAERMARTAYRYGVPPEAMNALQETMAQAY
                     NEAREHMEDARMEMEMQAEQSLQQEWGANYERNMGRAVAVLRRLTSETGVDADALLDN
                     PGLGSNPDAIRLLYQASRLLDEALHHTGNAAPSPAEEAMRMESDPSHPLYEAYMNVNH
                     PNHKYANELYDRLTTR"
     gene            complement(2083..2364)
                     /locus_tag="PK077_07515"
     CDS             complement(2083..2364)
                     /locus_tag="PK077_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24186.1"
                     /translation="MNQDTTLQQEASVREARFKRRQLLRVFDTPDGREALTFLEARFQ
                     TDLPVFQGSPGNYDPLDAMRRDAYREIFLYIRRQLQLAIKETTEEEKND"
     gene            complement(2361..3951)
                     /locus_tag="PK077_07520"
                     /pseudo
     CDS             complement(2361..3951)
                     /locus_tag="PK077_07520"
                     /inference="COORDINATES: protein motif:HMM:NF023657.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
     gene            complement(3960..4166)
                     /locus_tag="PK077_07525"
     CDS             complement(3960..4166)
                     /locus_tag="PK077_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24187.1"
                     /translation="MKPSTPSASAPEQTIPVKAESVEQEVGEDYQARERQRQGMISTI
                     LARRHSSAGQGEVNPRTLLRKTLG"
BASE COUNT         1052 a         1398 c         1138 g         1045 t
ORIGIN      
        1 cctgttaatc aagttcctct ccggaacacc tgccttactt gccttccgct gtcttgggtg
       61 cgtatttctt gaaaaattca acgtactggg cgggagtacg gccgggaaca taacccgtgc
      121 ggcccagctc cttgccatcc gcatccacca ccaccatggc cggcacgccg gtaatgttat
      181 acgtcttggc ttccttggac ttgctgaaat ccgaactgcc gaactcaaat ttgacgccaa
      241 tggccacgcc gtccatgcct tgtttgaaat cctttttgga aaggatttct ttctccagct
      301 taacacaaaa gccgcaccag gacgttcccg tgaaaagaag aacaatggga aggccggttt
      361 ccgccgcctg cttttgagcg cttttcaggc tcttgcccca gcggaatttc actttcgccg
      421 ccttggcggc cttggctgcg gcttccttgt ccgtgggaac gctttgagcc atggaaatgc
      481 cggaaacgct gaaccccagc acagtacaca atgcaaaaat aaaatgtctg ttcatcatac
      541 tctgtgaaat gttcctctca gactagcaga aatactttgt ataggaagag aaatcggtgt
      601 ctttttccta gaaggaacgc ttccgtcccc tgtccccaaa atgaggcgtt ctcaaaaaaa
      661 gtattgctct ttcctcctta atcgggtaat aatccgcccg cacttacaaa cgcgcggtta
      721 gctcagtggt agagcaacac cttgacatgg tggaggtcgt aggttcaaat cctataccgc
      781 gtaccatttt tcttaaaacc ccgttccttt ccggacgggg tttttcattg cctccctcca
      841 gtcaatagac atagcatcag ccttccccgt aacaggctca attgaaaaat gccggcataa
      901 agagacggtt aatgcagaag tttgcgttga taagaaaaaa taccggatat ctccctcttt
      961 actcacttac agtgaaagat atgatgagca tttaacctca ttagtctccc atacagggaa
     1021 aaatgttttt ccaacttttt ttgaacacaa gccccttccc gggacgctaa tagtaggagg
     1081 gcagcttaag gcctcagccc ttaccccctc cttccctgat gaaacaatga ctnnnnnnnn
     1141 nnaagcccgg cggaaagcat tccgccgggc ttgcttttta taagactccg ccggttaaaa
     1201 cgtccgggaa attaacgggt agtcaaacga tcatataact cattggcata tttatgattg
     1261 ggatggttca cattcatata cgcctcataa agaggatggg atggatccga ttccatccgc
     1321 atggcttcct ccgcagggga aggcgccgca tttcctgtat gatggagagc ttcatcaagc
     1381 aggcgtgatg cctggtacaa caggcggatt gcatccggat tggaaccgag tcccggatta
     1441 tccagtaaag cgtccgcatc cactcctgtt tcagaggtaa gccgccgaag aacggcgaca
     1501 gccctgccca tgttgcgttc ataattggca ccccattcct gctgaaggga ttgttccgcc
     1561 tgcatttcca tctccatccg ggcatcttcc atgtgctcac gggcctcgtt atatgcctgg
     1621 gccattgtct cctgaagagc attcattgcc tccggcggta ctccgtaacg ataggccgtg
     1681 cgggccatgc gttctgccag gtcagcattc cattcgcttt ccggcgtgga ttctggacgt
     1741 tccaggcgat attcttcctc cgactccggc aatccggcca gccggcggaa ccgcgccatc
     1801 tgttcttcat tctccacgcc gggataacgt cgcaggcgtt ccagctccgc gtaactcttc
     1861 gccagagcct ccggcgtctt aaacttggaa agagatttct ccatcccttt caactcatca
     1921 aaccgggcgt accaatccgg agcaaaacct ccgtcctcac ccagaagggg aggaaaaggt
     1981 tcagtttgaa ctgcggtttc ggacagcgcg tccgtctccg gggaaacgaa cccggaaccc
     2041 tccgcatccc tgaaaggagc ggccatgctg ttatcaatgg aatcaatcat ttttctcttc
     2101 ttctgtagtt tctttaatgg ctaattgaag ctggcggcgg atgtacagaa agatctccct
     2161 gtaggcatcc ctcctcatgg catccagagg atcgtaattc cccggacttc cttgaaaaac
     2221 gggcaaatca gtctggaaac gggcttccag aaaggtaagc gcctcacgcc cgtccggggt
     2281 gtcaaacacc cggagaagct ggcgcctttt gaaacgggcc tcccggacgg aggcctcctg
     2341 ctgcaatgtc gtatcctggt tcatttctga atcgcggtta actggtcaag caagggattg
     2401 agggaagcat aaggatcctc ttccgccgga gccagggagg ctccctgctg gaggtccgtc
     2461 cgttccttcc gcatggcacg gacatcggcc cagggcctca gcatgctctc cggggcgccg
     2521 tccacacggg cggacaggcg gaaacagtgg tcccagtcca catgatccgc caaatccggg
     2581 gcagcctgta tcatcatatt cagccgctga aggctgcggt ccatcccttc actctgcaag
     2641 cgcctgagca ccagggcaat ctttgactga tagacaaccc tgggttctcc cacggcaacg
     2701 gagccgtccc tccctaccct caataccgca cggggtggcc tgggaaactt gcccatccgg
     2761 aacagcaggg aaaaaatgcg tgtcattgtg gaatacagat cactcacaaa cagcgtgaag
     2821 gaaggagaaa acatcaagac gcgctcattc tcccgcgcca tgacctccgt ggcagtcata
     2881 ttgccgcggt gcccgctcca aagctccagc atgggcagat aataggcacg gcgtatcgca
     2941 tcctgcttct gtgtcagacg gtccatccca acatcatacc tgccctgcgt agcccattct
     3001 gccgccggcc cgcaaatcca cttccccaat ctggttggcg agctccagaa tacggggaaa
     3061 ggcggccacc tcgccaagag tatccagaat acggttcagg aactgcacct gctggatggc
     3121 gggaaacacc agcctgccgg gagccagacc gtacgggccg ctgccccact tcaaaaagcg
     3181 ggtcaccaga taagggaact ccatgtatcc cccttcctcc acgatcacct ggtcgtccag
     3241 agacaggtaa acgctttcaa acggcatgtg ggaggcctgc tccctgcggc ggctgcgccg
     3301 ggtgcgcggg cgcacaacat gcagaaacct cagagttgtg gcatacggat tgcctccgcg
     3361 ctccagaact tcccgagcct tgggccccag agctttcacc ccgaacatgg agcgtgcctg
     3421 atgagccgtg taggtaaact ccctgacgta ggtatccacc cggccttccg cattctccgc
     3481 acaggcgaac tgtccgcacg gaatattggt aaacaacagc cttccgtccg aggatatgcc
     3541 cgtaaacaga cttccggtcc ccaaagccac ccggtccaga aaacactcat gaatctccgt
     3601 ataaaaattg gaaacggaca attctttcag ggcaatttcc gaacactggt tataccaggc
     3661 ctccgcctcg tcgcctcccc ggtcatccgg agccgaccac ttgaaccata cgtcatggct
     3721 gggcgtaata taggacatat ggccgctggc cagcttctgg catgcctcca cagccgtagt
     3781 atccgtcatg cggtccatgg cgtccctgtt gggtagcgaa acctcccctt cccggttcag
     3841 gcggcgaggc agcacgtaat cccttagacg gtcccaccac gtctcccatg gcgcgcgctg
     3901 ggcggccagg gacttgtaca cggaattcaa ttccgcagtt ctttcttcca tggcagcgcc
     3961 tatcccagag ttttcctaag cagagtccgc ggattgacct ccccctgccc ggcggaagaa
     4021 tgacggcgcg ccagaatggt ggaaatcatt ccctgcctct gccgttcccg ggcctgataa
     4081 tcttccccta cttcttgttc cacgctctcc gccttgaccg ggatggtctg ctccggagca
     4141 gaagcggaag gagtggacgg tttcataaat cccataattt cttttgcttt ctattcatgg
     4201 ttgagactga ttccccttcc cgtttgccgg ataaaggctc cggaggatga cggacgcccg
     4261 cctcacagac aatccatgcg gcgggagcgt tcttttccca tggaaggaaa gggagctctc
     4321 tccacctgcc cggacaatcc gggcaagtgc ttgcatttat caaataaaca aatcggcaaa
     4381 aaatgcacct gaggaaaaac cggacgcata catgccattg gtggcatcaa agaggtcgcc
     4441 tccaaacgtg atgcattccg catccgtttc ctccaaaaca tagggcgcta tgatcaatga
     4501 atccttactt cagccggata cgccatccgc gctggacata tgcaacgcgg ccctctccaa
     4561 aataggggag gcacctctgg acgcgctgat tgccaatgaa tccacggcat cccgcctttg
     4621 cgttcttcat taccatccgg ccc
//
LOCUS       DAOMSU010000168         4553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1786_length_4553_cov_3.175189, whole genome shotgun sequence.
ACCESSION   DAOMSU010000168 DAOMSU010000000
VERSION     DAOMSU010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1786_length_4553_cov_3.175189"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..164
                     /locus_tag="PK077_07530"
     tRNA            87..164
                     /locus_tag="PK077_07530"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Asp,seq:gtc)
     gene            308..1867
                     /locus_tag="PK077_07535"
     CDS             308..1867
                     /locus_tag="PK077_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRN24188.1"
                     /translation="MSILSDRLSAEILQARQRVYTVGEPTPLQRLNLPGIQAPVYAKR
                     EDLGPIRAYKWRGAFNCMAALSQEARDKGIVAASAGNHAQGVALAARVLNCHATIFMP
                     RSTPEVKQTEVRRHGGRHVKIMLHGDCYDETAAAAHEYTETHGCTFVHPYDDLVTMAG
                     QGTLADEVVMSGEGPFDRAYVAIGGGGLAASVACWLKKFWPHIKVIGVEGIDQASMKT
                     SIEQGRRVNLDYVDVFCDGTAVHIPGELTYPLCRELVDEFVTVTNNEVCQAIRAMWES
                     SRVVPEPSGAMSLAGFLKQWNRGEVKPEEKSFVVISGANMDFTHLTQIARQAGIGNHE
                     TRYLRISMDSKRGQVLKYLRHIPQDTTLVDVQYGKTEGNTQHPVFGIAASDEDLDTIR
                     TTLKKKGIEFEDISEDDDVRFRMIHYQPELCQHPLFVHIEFPERAGAFLDFMERIADL
                     ASLCYFNYTYTGERVGRALVGMEFDTAEDRETIRKRMSAFSKNIIRSIREISPEAFQR
                     IMGSRQLTGNH"
     gene            1934..2503
                     /locus_tag="PK077_07540"
     CDS             1934..2503
                     /locus_tag="PK077_07540"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRN24189.1"
                     /translation="MIPPVILASQSPRRRELLEKTGIPFSIIVRDTEELKEASMPPQE
                     LCLHNAAAKAEAVFREHPDSTVIGADTLVFLEGFPLGKPEDEEEARSMLRKLSGRTHH
                     VCTAVAIRSPLGMKNLAVLTEVTFRELTEKDIRHYMELVDVMDKAGSYAFQEHGEMII
                     SSVRGDTDNVIGLPVRDVMKCLNDLGYRV"
     gene            complement(2607..3920)
                     /gene="hflX"
                     /locus_tag="PK077_07545"
     CDS             complement(2607..3920)
                     /gene="hflX"
                     /locus_tag="PK077_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRN24190.1"
                     /translation="MFEIREKPEMVERAMLVSIYFDPSEAGEKQAMLDELEDLVSNLG
                     IGIAGKHLVKSRDMHAKFLCGTGKAQEVKQLALDCRADCVVFDNMLSPSQQREWERLV
                     DECVIDREEVILDIFARRARTREASLQVELARMQYSLPRMARMWNHLDRQGGGSGGGK
                     GGGGAARGEGEKQIEVDRRLARARIEAIQRELVLVTRQRATQRKERERQAVATAAIVG
                     YTNAGKSSLLSLVSGSEVMARDMLFATLDTTTRKIELPHGQPLLLTDTVGFIRNLPHR
                     LVEAFKSTLEEAVLADFLIQVVDASDPEAVRHYETTLEVLNELGAGDKPMIVVLNKVD
                     LVPEERRGALETLLAPHFSGRVVPMSVKEGNGEGDLLNACVEMLESRVRRARFLIPYT
                     RSDLVAAMHSEGKVFSTEYVEEGTLLEAVLPVAFYNKLESFLADR"
     gene            complement(4270..>4553)
                     /locus_tag="PK077_07550"
     CDS             complement(4270..>4553)
                     /locus_tag="PK077_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24191.1"
                     /translation="SGAYQVSWNELPFNSLHALARECRQKQPAEFSSLADTYSKPLLI
                     FGRLTGTISSAQQEKALLQMDRSFIEKWNLWMNSLEAEEGLPGDDGKSS"
BASE COUNT          994 a         1371 c         1263 g          925 t
ORIGIN      
        1 atgatttctt gtcttgcatt tctgcgggag cgcctgtata agccttcccg tccccgccgc
       61 aaggcaggga cggcatccaa ggtcatggag cggtagctca gttcggttag agcgccagcc
      121 tgtcacgctg gaggtcgcgg gttcgagccc cgtccgcttc gccaccttgt tcagaagaaa
      181 cagccgcctc cgggcggctt ttttcgtcca gaaaacctga attccggaaa acaggccgcc
      241 ccccccggga acgttttact ggaaaaaggg gttggacttc agtagaatgg gggccagcat
      301 tacaatcatg agcatacttt cggaccgtct ttccgcggaa atactgcagg cccgccagcg
      361 cgtctacacc gttggcgagc cgactcctct ccagcgattg aatctgccgg gcatccaggc
      421 cccggtatac gccaagagag aggatctggg gcccatccgg gcctataagt ggcgcggcgc
      481 gttcaactgc atggccgcat tgagccagga agcccgtgac aagggcattg tggcggcttc
      541 cgccggcaat cacgcccagg gcgtggcgct ggcagccaga gtgctgaact gccatgccac
      601 cattttcatg ccgcgctcca cgccggaagt gaagcagacg gaagtgcgcc gccacggcgg
      661 caggcatgtg aagatcatgc tccatgggga ctgctatgac gagacggccg ccgccgccca
      721 tgaatacacg gaaactcacg gctgcacttt tgtccatcct tatgacgacc tggtgaccat
      781 ggccggacag ggaacgctgg cggacgaagt ggtgatgagc ggagaaggcc cttttgaccg
      841 cgcgtacgtc gccatcggcg gcggcggact ggccgcctcc gtggcgtgct ggctgaaaaa
      901 attctggccc catatcaagg tcatcggcgt ggaaggcatc gaccaggcgt ccatgaaaac
      961 ttccattgag cagggacgcc gggtgaatct ggattacgtg gatgtcttct gcgacggcac
     1021 ggccgtccac atccccggag aattaaccta tcccctttgc cgggaactgg ttgacgagtt
     1081 cgtgaccgtg accaataatg aggtctgcca ggccatccgg gccatgtggg aatcctcccg
     1141 cgtcgtgccg gaaccatccg gagccatgag cctggcagga ttcctgaaac aatggaaccg
     1201 gggcgaagta aagccggagg aaaagagctt cgtggtcatt tccggagcca atatggattt
     1261 cacccacctt acccagattg cccggcaggc gggcatcggc aaccatgaaa cgcgctatct
     1321 gcgcatttcc atggattcca agcgcggaca ggtgcttaaa tacctgcgcc acattccgca
     1381 ggatacgacg ctggtggatg tgcaatacgg caaaacggag ggaaataccc agcaccccgt
     1441 gttcggcatt gccgcttccg atgaagacct ggataccatc cgcacgacac tgaaaaagaa
     1501 aggaatcgaa ttcgaagaca tcagcgagga cgacgacgtg cgcttccgca tgattcacta
     1561 ccagccggaa ctgtgtcaac accccctgtt cgtccacatt gaatttccgg aacgcgccgg
     1621 cgccttcctt gacttcatgg aacgcatcgc ggatctggct tccctctgtt atttcaacta
     1681 tacctacacc ggggaacgcg taggccgcgc cctggtgggc atggaatttg atacagcgga
     1741 agaccgggaa accatccgga aacgcatgag cgccttttcc aaaaacatta tcaggtccat
     1801 tcgggaaatt tcccccgagg ctttccagag gattatggga agcagacagt tgacaggcaa
     1861 tcattgacag cccatcatcc agggagcttg agaaaaatcg gctccctgtt tttcatgacc
     1921 gcttttttct cccatgattc cgcccgtcat cctggcctcc caatcgcccc ggcggcgtga
     1981 attgctggag aaaaccggca ttcctttttc cattattgtc cgggacacgg aagaactgaa
     2041 agaggcttcc atgcccccgc aggaactctg cctgcacaat gccgccgcca aggcggaagc
     2101 cgtgttccgg gaacatccgg attccaccgt catcggcgcg gatacgctcg tttttctgga
     2161 aggctttcct cttggcaagc cggaagatga agaagaagcc cggtccatgc tccggaagct
     2221 ctccggacgc acgcaccatg tctgcacggc ggttgccatc cgttccccgc tgggcatgaa
     2281 aaacctggcc gtgctgacgg aagtaacgtt ccgggagctg acggaaaaag acatccgcca
     2341 ttacatggaa ctggtggacg tgatggacaa ggccggctct tacgcctttc aggaacacgg
     2401 ggagatgatt atttcctccg tgcgcgggga tacggacaac gtcatcggac tgcctgtcag
     2461 ggatgtgatg aaatgcctga acgacctggg ataccgggtt tgacccgtcc aggaatccgg
     2521 atactgtctc cggaagaaaa aactcttccc gccccggaac ggcctttgga acgcaaggca
     2581 aagccccagg cttcaggaac aggggattac ctgtctgcca ggaatgattc cagtttattg
     2641 taaaaggcca caggaagcac ggcttccagc aaggtccctt cctctacata ttccgtagag
     2701 aaaaccttgc cttcactatg catggcggcc accaggtcgc tgcgggtata gggaatcagg
     2761 aaccgggcgc gccggacacg gctttccagc atttccacgc aggcattcag caggtctccc
     2821 tcaccattac cttctttcac ggacatggga accaccctgc cgctgaaatg aggcgccagc
     2881 agcgtttcca gggccccgcg ccgttcttcc ggcaccaggt ccaccttgtt tagaaccacg
     2941 atcatgggct tgtcacccgc acccagttca ttgagaacct ccagagtcgt ttcataatgg
     3001 cgcacggctt ccggatcgga agcgtccacc acctgaatca ggaaatccgc cagaacggct
     3061 tcctccagcg tggacttgaa cgcttccacc agacggtgcg gaaggttgcg gatgaagccc
     3121 acggtatccg tcagcagcag cggctgccca tggggaagct ctatcttccg cgttgtggtg
     3181 tccagcgtgg caaaaagcat atccctggcc atgacttcag acccggacac cagggacagg
     3241 agggaggatt ttccggcatt ggtataacct acaatggcag ccgtggcgac agcctggcgc
     3301 tcccgttcct tgcgctgcgt ggcacgctga cgggtcacca ggaccagttc cctttgaata
     3361 gcctcaattc tggcgcgcgc caggcgacgg tccacttcaa tctgcttttc cccttccccc
     3421 ctggcggctc cgccgcctcc cttgccgccg ccggagcccc cgccctggcg gtctaggtgg
     3481 ttccacatgc gggccatgcg cggcagggaa tactgcatgc gggccagttc cacctgcagg
     3541 gaggcttccc gtgtccgggc gcgcctggca aagatgtcca gaatgacctc ttcacggtca
     3601 atgacgcatt cgtcaacaag ccgttcccat tccctctgct gggatgggga gagcatattg
     3661 tcaaaaacaa cgcaatccgc ccggcaatcc agcgccagct gcttcacttc ctgcgccttt
     3721 ccggtgccgc acaggaattt ggcgtgcatg tccctggact tgacgaggtg cttccctgcg
     3781 atgccgatgc cgagattgga gacaaggtct tccagctcat ccagcatggc ctgtttttcc
     3841 ccggcctcgg agggatcgaa gtaaatggaa acgagcatgg cccgttccac catttccggt
     3901 ttttcgcgga tttcaaacat acagggaagt taacggaact gcttgctgga ggtaggctgg
     3961 taatccttca gagcgaagtc cgcgggctta tgacaatcac aactgcatga agagaggaag
     4021 ccggccacgc atgccggcgt cagaaagctg atcagtctca tgcggctatg agacccgcac
     4081 gaagtaccct aggcaagcaa attttacttc atcaccatta cttttctcca aatccggaac
     4141 aaaggcattt gcccggacgg ttttcctttt ttccggcccg ggggcagccg gcaaaacacg
     4201 cggcgggggc agaaacctgc cggcgacagc aatccgccgc ctttcaaggg actttattcc
     4261 cttccggaat caacttgatt ttccatcgtc tcccggcagc ccttcctccg cctccagact
     4321 gttcatccat aaattccatt tttcaatgaa agagcggtcc atctgaagca atgctttttc
     4381 ctgctgtgcg gaggaaatgg ttcccgtcag cctgccaaaa ataagcaaag gcttggaata
     4441 cgtatccgcc agggaagaaa attccgcagg ctgcttctgg cggcattccc gcgccagagc
     4501 atgcagggag ttgaacggaa gctcgttcca actgacctgg tacgcaccgc ttc
//
LOCUS       DAOMSU010000169         4526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1802_length_4526_cov_2.847014, whole genome shotgun sequence.
ACCESSION   DAOMSU010000169 DAOMSU010000000
VERSION     DAOMSU010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1802_length_4526_cov_2.847014"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..567)
                     /locus_tag="PK077_07555"
     CDS             complement(133..567)
                     /locus_tag="PK077_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HRN24192.1"
                     /translation="MEITIIYGTETGNSESLAQDARAKLTKLGHQAKVVDMEGMTVEQ
                     LKNAGTLLVITSTWGDGEPPTNAEALHQELKDSSEDLSEVSYAVFALGDEFYENFCQA
                     GKDFDSFLERLKAQRLLPVVLSNGDHDDTFPEWVDAIAEKLS"
     gene            complement(711..1190)
                     /locus_tag="PK077_07560"
     CDS             complement(711..1190)
                     /locus_tag="PK077_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24193.1"
                     /translation="MFLKSCSTPAAAPEPVAGWYEGTLYEDKPFYITKNAISWTSGRK
                     KADFGRWTAVSVYDPSIRMQAEGGRFSITPGYGWDGVTWGSTPPDMLLPSLFHDAMLH
                     AKMNGAPISRSQIDLAFYDIMTSQNSCRKGLYYRFARLFGWCFTFPQHPNTLIIKRE"
     gene            complement(1284..2141)
                     /locus_tag="PK077_07565"
     CDS             complement(1284..2141)
                     /locus_tag="PK077_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRN24194.1"
                     /translation="MKSRPRRPFLNKLAIIFVPILMIGFSLFMWAWQVEPRRVETVTA
                     AMEMPQWKRKEGPPLRIAIAGDFHLRPNGGDLAHKYMETIMEARPDMIFLLGDYANGH
                     TRESSMAPETAREYFKMLKAPLGIFAVQGNHDQYYGWNLWRNMFSGLGILPMWNDSLL
                     LHLPGGRELQLSSVRDDYHLRIRPEELPLRFSPDIPHILLSHVPDIFPLLAPGTADLV
                     ISAHTHGGQICLPGGRALANISREKVKFSYPWSQLNGTPFLITRGLGCSVLPLRFCCP
                     PEIIVLEIQ"
     gene            2295..2987
                     /locus_tag="PK077_07570"
     CDS             2295..2987
                     /locus_tag="PK077_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015573757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24195.1"
                     /translation="MQFTHEIEQMCCVKKGCNHGPAPIPQEGNWTQSREITDISGLTH
                     GVGWCAPQQGTCKLTLNVKNGIIEEALVETSGCSGMTHSAAMAAEILPGKTVLEAMNT
                     DLVCDAINTAMRELFLQIVYGRSQSAFSEGGLPIGAGLEDLGKGLRSQIGTLYGTRAK
                     GPRYLEMAEGYITKLALDEDGEIIGYEFVRMGPMMEMIKKGVDANEAVAKCTGNYGRF
                     DEAAKYIDPRHE"
     gene            3033..4040
                     /locus_tag="PK077_07575"
     CDS             3033..4040
                     /locus_tag="PK077_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004846171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGGtGRT protein"
                     /protein_id="HRN24196.1"
                     /translation="MPLFESYDRRIKQINEALAKYGIGSIEEAEQLCLSKGINPREIV
                     NGIQPIAFENAGWAYVVGAAIAIKKGCTKAADAAEAIGEGLQSFCIPGSVADERKVGL
                     GHGNLAAMLLRDETECFCFLAGHESFAAAEGAIGIAKSANRVRKQDLRVCLNGLGKDA
                     AYIISRINGFTYVQTKFDYATGKLNIIQEKAFSKGPKAAVRVYGCDDVREGVAVMWNE
                     GVDVSITGNSTNPTRFQHPVAGTYKKECNEKGKKYFSVASGGGTGRTLHPDNMAAGPA
                     SYGMTDTMGRMHSDAQFAGSSSVPAHVEMMGLIGMGNNPMVGASVAVAVAVEEAAKQN
                     A"
     gene            complement(4166..>4526)
                     /locus_tag="PK077_07580"
     CDS             complement(4166..>4526)
                     /locus_tag="PK077_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24197.1"
                     /translation="GKLDAAAQLQRRVEPLAQKAGAPKKADYSLRPGEQRDFTLQRAY
                     KAHERQKTVVGTVPVLPSSHGTQPGKTVVPASASRPEIRSVKPSSARQESVSRRSVPS
                     RGLPPRADRDGRFRIGS"
BASE COUNT         1040 a         1243 c         1257 g          986 t
ORIGIN      
        1 attatccccc ccttgcgccc gaaccggaaa aagagagttc ctgctttttt taggaaaaag
       61 tgcttcctcc aggcgttcgt cccggaagaa gtgctctttt gaagataaga tgactgaaat
      121 cttgaaaaaa catcaggaga gtttttccgc gatggcatcc acccattccg ggaaggtgtc
      181 atcatggtct ccattggaaa gcactaccgg cagaagcctt tgcgctttca aacgttccag
      241 gaaggaatcg aagtccttgc cagcctggca gaagttttca taaaattcat ctccaagggc
      301 aaaaacggcg tagcttacct cggacaaatc ttccgaagag tccttcaatt cctgatggag
      361 ggcctctgcg ttggtgggag gttctccgtc cccccacgta ctggtgatga ccagcagcgt
      421 tcccgcattc ttcaactgtt ccaccgtcat accttccatg tcaaccacct ttgcctgatg
      481 gccgagcttg gtcaattttg cccgcgcgtc ctgggccaga ctctccgaat tgccggtttc
      541 cgttccgtag ataatagtga tttccatgcg ttctgattat tggttcgaca acgaaagtta
      601 gattgaacta attaaacagg caagacaaaa cagcggaacg acggttgaat ttttcttcct
      661 tgttcatcct tccgggtaac tggttttccg gaaaattctc cgatgccata ctactctcgt
      721 ttgattatca aagtattcgg atgctgtgga aaggtgaagc accagccgaa aagacgcgca
      781 aaacggtagt ataatccctt gcggcaggaa ttttggctgg tcatgatatc gtaaaaagcc
      841 aggtcaatct ggcttctgga aataggggct ccattcattt tggcgtgcag catggcgtcg
      901 tggaacaggg aagggaggag catatccgga ggggtggacc cccaggtgac gccgtcccag
      961 ccatagccgg gagtaatgct gaaacgaccg ccctccgcct gcatgcgtat ggatgggtca
     1021 tacacgctga cggctgtcca gcggccaaaa tccgcctttt ttcttccgct ggtccagctg
     1081 atggcgtttt tagtgatgta gaaaggcttg tcttcataga gggttccttc ataccagcct
     1141 gccacgggtt ccggagccgc ggctggcgtg gagcaggatt tcagaaacat ggctgcacat
     1201 attgttgtga acaatatgta aatacaatat gaataagaaa taaagggcat gaggggacgg
     1261 agcgttatcc ggatagttgt ttcttattgg atttccagca cgatgatttc cggagggcag
     1321 cagaagcgca ggggaagaac gctgcatccc aaacctcggg taatgaggaa gggagttccg
     1381 ttcagctggg accagggata ggaaaatttg accttctctc gtgagatgtt tgccagggcc
     1441 ctgcctccgg gaaggcatat ctggcctccg tgggtatgcg cgctgatgac gaggtccgcc
     1501 gtgccgggag ccagcagggg aaaaatgtcg ggtacatgtg aaagaaggat atgcggaata
     1561 tccggggaaa aacgcagagg caattcctcc ggcctgattc tcaggtggta gtcgtccctg
     1621 acggaagaga gctgcagctc acggcctccg ggaaggtgca gcagcaggga atcattccac
     1681 atgggcagga tgcccagccc ggaaaacatg ttgcgccata agttccagcc gtaatactgg
     1741 tcatggttgc cctgcacggc aaaaatgccg agaggggctt tcaacatctt gaaatattcc
     1801 cgggccgtct ccggggccat gctgctctcc ctcgtgtggc cgttggcata atcccccagc
     1861 aggaaaatca tatccggccg agcttccatg attgtttcca tgtacttgtg tgccagatcg
     1921 cctccgttcg gacgcagatg gaaatccccg gcaatcgcga tgcggagggg agggccttct
     1981 tttctcttcc actgaggcat ctccatcgca gccgtgacgg tttccacacg cctgggttcc
     2041 acctgccatg cccacatgaa taaggagaag ccaatcatca atattgggac aaagatgatt
     2101 gccaatttgt tgagaaaggg tcggcgtggt cgtgacttca tgcagggcag ggctcctgac
     2161 tgattatgag gccttttaac cgattttcaa tcattcgtgt ttgctaatcg ggaatttgct
     2221 ggtatgatcc gtcccgctca aggtagggct gtccgatgac tgcagctcta tcttcatatt
     2281 aacatcctaa taccatgcag tttacacacg aaatcgaaca aatgtgttgc gtcaagaaag
     2341 gctgcaacca tggtccggcc cctatcccgc aggaaggcaa ctggacgcaa tccagggaaa
     2401 tcacggatat ttccggcctg acccacggcg tgggctggtg cgctccccag cagggcacct
     2461 gcaaactgac gctgaatgtc aaaaacggca tcattgaaga agcactcgtg gaaaccagcg
     2521 gctgctccgg catgacccat tccgccgcca tggctgcgga aatcctgccc ggcaagaccg
     2581 tattggaagc aatgaacacg gacctcgtgt gcgacgccat caacaccgcc atgcgcgagc
     2641 tgttcctgca gatcgtctat ggccgcagcc agagcgcttt ctctgaagga ggccttccca
     2701 tcggcgccgg tcttgaagac ctcggcaagg ggcttcgctc ccagattggc accctgtacg
     2761 gcacccgagc caagggcccc cgctatttgg aaatggctga aggctacatt accaagcttg
     2821 ccctggatga agacggtgaa atcatcggct acgagtttgt gcgcatgggg ccgatgatgg
     2881 aaatgatcaa gaagggcgtg gacgccaacg aagccgtcgc caaatgcacg ggcaactacg
     2941 gtcgtttcga cgaggcagcc aagtacattg atcctcgtca cgaatgagat tgaattaaaa
     3001 agagtatctt aacttcaagc actattacta ttatgcctct tttcgaatcc tacgaccgcc
     3061 gcatcaagca gatcaatgaa gccctggcca aatacggcat cggctccatt gaagaagcgg
     3121 aacaactctg cctttccaag ggaatcaacc cccgtgaaat tgtgaacggc attcagccca
     3181 tcgcgtttga aaacgccggc tgggcctatg tggtgggagc cgccatcgcc atcaagaagg
     3241 gctgcacgaa agccgccgac gctgctgaag ccatcggaga aggccttcag tccttctgca
     3301 ttcccggctc cgttgcggat gaacgcaagg tgggcctggg ccacggcaac ctggccgcca
     3361 tgctcctgcg cgatgaaacg gaatgcttct gcttcctggc cgggcacgaa tcctttgccg
     3421 ccgctgaagg tgccatcggt attgccaagt ccgccaaccg cgtccgcaag caggatctcc
     3481 gcgtctgcct gaacggcctc ggcaaggatg ccgcgtacat catctcccgc atcaacgggt
     3541 ttacatatgt gcaaaccaag ttcgactatg ccacgggcaa gctgaacatc atccaggaga
     3601 aagctttctc caaaggcccc aaagccgccg tgcgcgtgta tggctgcgat gatgtgcgcg
     3661 aaggcgtggc tgtcatgtgg aatgaaggcg tggacgtttc cattaccgga aactccacca
     3721 accccacccg cttccagcac ccggtggccg gcacctacaa gaaggaatgc aatgagaagg
     3781 gcaagaagta cttctccgtg gcttccggcg gcggcacggg ccgtaccctg cacccggaca
     3841 acatggcggc cggtcccgcc tcctacggca tgaccgacac catgggccgc atgcactccg
     3901 atgcccagtt cgccggttct tcctccgtgc ccgcccatgt ggaaatgatg ggcctcatcg
     3961 gcatgggcaa taacccgatg gttggcgctt ccgtagcggt ggccgtggct gttgaagaag
     4021 ccgccaagca gaacgcctga gcgagcagct gagcaaaaaa ccgcagagcc gctttcccga
     4081 ttagggaaag cggctctttt agcaattgaa agaaaaaggg ggatgtcccc atttccggcc
     4141 ccctcttcct gcccttgtat ccgcatcagc ttccgatacg gaatcttcca tcccggtccg
     4201 cccggggagg aagtccgcgc gaaggaacgg atcttctgga aacggattcc tgcctggcag
     4261 aggacggctt gacggagcgg atttccgggc gggaggcaga agcgggaaca accgtttttc
     4321 ccggttgcgt gccgtgggag gaagggagaa caggaacggt tccgacaacc gttttttgcc
     4381 gttcatgggc cttataggcg cgttgcaggg taaaatcccg ctgttctccc ggtctcaggg
     4441 aataatccgc ctttttggga gctccggctt tttgcgccag aggttccacg cgccgctgca
     4501 gctgtgcggc ggcatccagt ttgcct
//
LOCUS       DAOMSU010000170         4493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1819_length_4493_cov_2.484452, whole genome shotgun sequence.
ACCESSION   DAOMSU010000170 DAOMSU010000000
VERSION     DAOMSU010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1819_length_4493_cov_2.484452"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1135)
                     /gene="pheA"
                     /locus_tag="PK077_07585"
     CDS             complement(<1..1135)
                     /gene="pheA"
                     /locus_tag="PK077_07585"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HRN24198.1"
                     /translation="METTHHGEDASPDNSPRQQKNGNTDEQTIALTKARLAIDEVDAR
                     IVELLKKRAEWVHEVGRIKKEKNSPIFVPERETAMLNKLNRLNAGVLPEASLQAIYRE
                     IISCSFFLEGGLTIAYLGPKGTWSHQAALKQFGKSCELIPCQSFKDVFDMVDRGKAQY
                     GVVPVENSSEGSVTAVMDLFVTSPLKICAQINLNIRNSLMADIPREHIRILYSHPQVL
                     GQTRNWIQRHFPNAELVETSSTTKASILAKENAAMGAASLGCPLAAELFGLNILEEDV
                     QDQSCNTTRFAVIGRQETQPSGRDRTSLLIRIQHKPGTLAEVVNCFQRHNNNLIRIES
                     RPSKVINWEYVFYIDAAGHIQESPLRETLPELEQAANAAGWVDV"
     gene            complement(1508..2479)
                     /locus_tag="PK077_07590"
     CDS             complement(1508..2479)
                     /locus_tag="PK077_07590"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRN24199.1"
                     /translation="MNLTTDDADGKIRLDQYLAAHLPELSRSRIQSLIKSGDVLVNGS
                     PAKPKNPVSRGDSITVRIPEPEPAEAQPQDIPLDILYEDEDVVVINKESGMVVHPAAG
                     NPDGTIVNALLHHCGDLSGIGGVERPGIVHRLDKDTSGCLVIAKNDGAHQSLTAQFAA
                     RSTEKRYLAVVQGIPSQSSGTVFTHIGRHPVNRLKMAVVNPGSGKAAITDYDLLCADP
                     STDSSLVLCTLHTGRTHQIRVHMLHLGHPLIGDPIYAKPARQKAKPGRLMLHAWRLGF
                     DHPRTGKRMDFEAPVPPEYTPWLQLFPNGLYGAIPEPRPVQEDGGRN"
     gene            complement(2496..3275)
                     /locus_tag="PK077_07595"
     CDS             complement(2496..3275)
                     /locus_tag="PK077_07595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HRN24200.1"
                     /translation="MSKIELHTAISFLDGILSVNSIPDAPRALNGLQLENGGTVSKVA
                     AAVDGSEKAIHAALETGADLLLLHHGIFWQPMQPITGIAYRKLKAAMDGNLAIYAAHL
                     PLDVHPAYGNNALLAKACGLQPCSHDGLDYHGVSLGTHGEFPGTCAELEQKLEIVLGA
                     PVQAFWKDSPEAPAGDVFICTGGAGDDLAQAAALGCRTYVTGEGSHWNIPLAQELGVN
                     LVFGGHYFTETFGVKALGLLLKDVYGLDYTFIDLPPSAYSH"
     gene            complement(3289..>4493)
                     /locus_tag="PK077_07600"
     CDS             complement(3289..>4493)
                     /locus_tag="PK077_07600"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HRN24201.1"
                     /translation="SVSIRNFTGRLSGDGKSLQMEEQPVTLRIYPPSDNGSLSELVKA
                     TVSDRKAVFELAQTTETNKEIPGVRNIPLDLGLLRSKQLTDIGDYKITGLSIRKSFQD
                     YPTIARFIQGDEKKGIKPTWLYTAEGARYQEANPEHLISLLKTVKQGKVAGFASDKVT
                     DLSVYGLDNPRMTLTMSLLPKPGEEPRPPVTVFFSKGADGSWYARQAGKPTVAILDNA
                     YMKDLLTDALAWKKKRLFSFSRFDLREMQLERIGSGGPLVLKFDRLDDSWTASKDGKD
                     ETLNINPNRANRYLDELEKMEVSAWLPYTDATAHEALKNPVFRLKLILQSYKEAAPVQ
                     AQSGSSDITFAPEPETEDKVITLEIAPAGEAGYSRFYYGRVNTTPNYFILNMDAVRLL
                     GASLSEDN"
BASE COUNT          816 a         1167 c         1479 g         1031 t
ORIGIN      
        1 agacatccac ccagcccgcg gcgttcgcgg cctgttccag ctccggaagg gtttcccgga
       61 ggggggattc ctgaatgtgg ccggcggcgt ctatgtagaa gacgtattcc cagttgatga
      121 ctttggacgg gcgggattca atgcgtatca ggttattgtt gtgccgctgg aagcagttga
      181 ccacttccgc cagggtgccg ggcttgtgct ggatgcggat gagcagggag gtgcggtccc
      241 tgccgctggg ctgcgtttcc tggcgtccga tgacggcaaa acgggtggtg ttgcaggact
      301 ggtcctgaac gtcttcttcc aggatgttca ggccgaacaa ttccgcggcc agcgggcagc
      361 ctagggacgc cgcgcccatg gcggcgttct ccttggcaag aatgctggct ttcgtagtgg
      421 aggacgtttc cacgagttcc gcgttcggga agtgccgctg gatccagttc ctcgtctggc
      481 cgaggacctg ggggtgggaa tacaggatgc ggatgtgttc ccgcggaata tccgccatca
      541 ggctgttgcg gatgttcaga ttgatctggg cgcagatttt gaggggtgag gtgacgaaaa
      601 gatccatcac ggcggtgacg gagccttccg aagaattttc cacgggaact acgccgtact
      661 gggcctttcc ccggtccacc atgtcaaaca cgtccttgaa gctctggcac ggaatgagtt
      721 cgcagctttt tccgaactgc ttgagagccg cctgatggct ccaggtccct ttggggccca
      781 ggtaagcaat ggtcaagccg ccttccagga agaaggagca ggagatgatt tcacggtaaa
      841 tggcctggag ggaggcttcc ggaagcacgc ccgcattcag gcggttcagt ttgttgagca
      901 tggccgtttc ccgttcggga acgaagatgg gggaattttt ttccttttta atgcggccga
      961 cttcatgcac ccattcggcg cgttttttta acagctctac aatccgggcg tccacctcgt
     1021 caatggccag gcgcgccttt gtcagggcga tggtctgttc atccgtgttt ccgttttttt
     1081 gctgcctggg ggaattgtcc ggagaggcat cctccccgtg gtgcgtggtc tccatgtctc
     1141 tgtgaaagct gtgtggttaa agccgtgtca gggtagcggt tccggggggg caaatcaaga
     1201 ttaaagggga ggtctccgcc gcggattctt cttgcatgcg cggaagatgc cgggcaggtt
     1261 catttctcct tgattgatga aaagaatatt gttcccggct gactgtgacc gtggacgctc
     1321 ctgccggaaa gggggcgtaa gaaggtgaac ggcgggtctg ttgccgcatc tttgtccgca
     1381 aggatggtgt cgccggatga cggcggattg ctgacgaagg ccgggccagc tctgggattt
     1441 gaatatggcg ctttcccgta ttctgtccgt tgaagcggct tccgctgccg ggaaatggcg
     1501 gggaggttta gttccgtcct ccgtcttcct gtaccgggcg cggctccggg atggccccgt
     1561 aaagcccgtt ggggaagagc tggagccacg gcgtgtattc cggcgggacg ggcgcttcga
     1621 aatccatgcg ctttcccgtg cgcgggtggt cgaagcccag ccgccacgcg tggagcatga
     1681 gtctgccggg ttttgccttt tgccgggcgg gtttggcgta aatgggatcc ccgatgagcg
     1741 gatggcccag atggagcatg tgcacgcgta tctggtgggt tctgcccgtg tgcagcgtgc
     1801 acagaaccag ggaggagtcc gtggacgggt ctgcgcagag caaatcgtag tccgtaatgg
     1861 cggctttccc ggaaccggga ttgactactg ccattttcag gcgattgacg ggatggcggc
     1921 cgatatgggt gaagaccgtg ccggaacttt gggaaggaat accctggacg acggccagat
     1981 aacgcttttc cgtgcttcgg gccgcgaact gcgccgtcag ggactggtgg gcgccgtcat
     2041 tttttgcgat gactaggcag ccggaggtgt ccttgtccag gcggtggacg atgcccgggc
     2101 gttccactcc gccgatgccg gagaggtccc cgcagtggtg gagcagggcg tttacgatgg
     2161 tgccgtccgg gttgccggcc gcgggatgca cgaccatgcc gctttccttg ttgatgacga
     2221 ccacgtcttc atcttcatac aggatgtcca gggggatatc ctgaggctga gcttccgccg
     2281 gttccggttc cggaatgcgg acggtgatgg aatcaccgcg ggagacgggg tttttaggct
     2341 tggcgggaga gccgttgacc aacacatccc cgcttttgat caggctctgg atcctggagc
     2401 gggaaagctc cggaaggtgg gcggcaaggt attgatccag gcggattttc ccgtcggcgt
     2461 cgtccgtagt cagattcatg ttccgtggcg tttggtcaat ggctgtaggc ggagggaggc
     2521 aggtcaataa aggtataatc cagcccgtaa acatccttga gaagaagccc cagggccttt
     2581 acgccgaacg tctccgtaaa gtagtggccg ccgaagacca gattgacgcc cagttcctgg
     2641 gccaggggga tgttccagtg ggagccttcc ccggtcacgt aggtgcggca gccgagggcc
     2701 gctgcctggg caagatcatc tcccgcgcct ccggtacaga tgaagacatc tccggccgga
     2761 gcctccgggg aatctttcca gaaggcctgc acgggcgcac cgaggacgat ttccagtttt
     2821 tgctccaatt ccgcgcatgt acctggaaat tctccgtgcg taccgaggga aacgccgtgg
     2881 tagtccagcc catcatggga acatggctgg aggccgcagg ccttcgccag cagggcattg
     2941 ttgccgtatg cgggatggac gtccagagga aggtgggcgg cgtaaatcgc caggttcccg
     3001 tccatggcgg ctttcagctt ccggtaggcg atgccggtaa tgggctgcat gggctgccag
     3061 aaaatgccgt ggtgaagaag gagaagatcc gcccctgttt ccaaggctgc atgaatggcc
     3121 ttttctgagc cgtccacggc ggcggccacc ttggaaacgg tgcccccgtt ttccagttga
     3181 aggccgttca gggcgcgggg ggcgtccgga atggaattca cggagaggat tccgtccaga
     3241 aaggagatgg cggtgtgcag ctcaatcttg ctcatgcggg aggagtggtt agttgtcttc
     3301 cgaaaggctg gcgccgagca gacgtacggc gtccatgttc agaataaaat aattcggggt
     3361 tgtatttact ctgccgtaat aaaatctgct gtatccggcc tctcccgccg gggcgatttc
     3421 cagcgtgatg actttgtcct ccgtttccgg ttccggggca aaggtaatgt cgctggaacc
     3481 ggattgggcc tgcacggggg ccgcctcctt gtaagactgg aggatgagtt tcaggcggaa
     3541 aacgggattt ttcagggctt catgcgctgt ggcgtccgta taggggagcc aggcgctcac
     3601 ctccattttt tccagttcat ccagatagcg gttggctcgg ttcgggttga tattcaaggt
     3661 ttcatccttc ccgtctttgg aggctgtcca agaatcgtcc agacggtcga atttcagaac
     3721 cagggggcct ccggaaccga tgcgttccag ttgcatttct ctgaggtcaa acctgctgaa
     3781 ggagaagagc ctcttttttt tccaggccag tgcatccgtc agcaaatcct tcatgtaggc
     3841 gttgtccagt atggcgacgg tgggttttcc ggcctggcgg gcataccagg aaccgtccgc
     3901 acccttggag aaaaacaccg tcacgggggg gcgtggctct tcaccgggct tgggcagaag
     3961 ggacatggtg agcgtcattc tgggattatc cagcccgtac acggaaaggt ccgtaacttt
     4021 gtccgaagcg aagccggcca cttttccctg tttaacggtt ttcagcagag aaatgagatg
     4081 ctccggatta gcttcctggt aacgggcgcc ctctgccgtg tacagccagg tgggcttgat
     4141 gccttttttc tcatccccct gaatgaagcg ggcgatggta gggtaatcct gaaaactttt
     4201 acggatggaa aggccggtaa tcttgtaatc cccgatgtcc gtcaattgct tggaacgcag
     4261 aaggcccaga tccagcggaa tattgcgtac gccggggatt tccttgttgg tttccgtcgt
     4321 ttgcgccagt tcaaagacgg ccttgcggtc ggaaacggtg gccttgacca gttcggacag
     4381 gctgccgttg tccgagggag ggtaaatccg gagcgtgacg ggctgttcct ccatttgcag
     4441 ggatttgccg tcccctgaga ggcggcctgt gaaattcctg atggagacgg agg
//
LOCUS       DAOMSU010000171         4493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1820_length_4493_cov_2.300811, whole genome shotgun sequence.
ACCESSION   DAOMSU010000171 DAOMSU010000000
VERSION     DAOMSU010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1820_length_4493_cov_2.300811"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="PK077_07605"
     CDS             <1..348
                     /locus_tag="PK077_07605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24202.1"
                     /translation="WNVSEVKSHPGLELYLYVDRTAKMELYAGRIQKGAAPALHLPED
                     ADQDLLAGLSGQQLEKPRGGGLAQWRKLPNDHYGDCVKIGQVSWWVRRGDFYAEEMNA
                     IEERKQNEGVPEE"
     gene            417..707
                     /locus_tag="PK077_07610"
     CDS             417..707
                     /locus_tag="PK077_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24203.1"
                     /translation="MNPIVQAYVENYDLPDLQGMLREKLAILEGRKEITGASTGGGTS
                     YTAQETMNLKDHIACLQEAITVKKMEEGDFSGLAAADDGVREVRFDHTITRF"
     gene            710..2236
                     /locus_tag="PK077_07615"
     CDS             710..2236
                     /locus_tag="PK077_07615"
                     /inference="COORDINATES: protein motif:HMM:NF016991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRN24204.1"
                     /translation="MGRNRRKVYAGARRGHGARVKMNREPETARREMWGGYAAALQFG
                     GSSVLYWPTLDSRFEVDSWTLDRVWRNARNLEANSGLAGKAVADVVELLGWLVPHACT
                     ADEDWNHEADQIFMNRAVNPELFDARGELNFFTAQIWSERQRVIDGDMLTVLTSGPDD
                     GGAFAFYEAPQVQSPADGGQAWNCGVMRDKNGRTAAYGLRHPDKGEVTVIPARDAILY
                     RHNMGGGKPRGLSDLHRAIRNLHDEADIVGYVKQSAKLAASVGLVETGDAEKRPGMGT
                     VGKVSVGPDGRRVEQVLGGPTVHQLPPGRDLKVLTDNRPSPNVMALLKHLMDEVAYGI
                     GLSPALLWEPDKLGSGGIRFVMQKLKRWLKIRHAYRQMWCVRVWRFMLAREMALGRLR
                     LCRDPHWVRCLWTPMSDMTIDLGREGNLMINLVDSALADQDGWCLANYGCTFEEIVNN
                     KIRNLKMAKEACARNGLTLQEVIPGANRGGVAAAAEKPEDEEPGTGDGEEEDGLHPHE
                     "
     gene            2281..3399
                     /locus_tag="PK077_07620"
     CDS             2281..3399
                     /locus_tag="PK077_07620"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HRN24205.1"
                     /translation="MNKIVFAQMAARLEGGAGEQKKTGMLAFSRIMEAEEKVGVATIS
                     GYIGYGNATVDEFTKHLEELKAEGCTKFEVILNSMGGNLFEASGIYDIIKGCGMEVTA
                     KIYGVAASAATLIACAAGRVLISENSRYMVHRARGCAVGTVEEIEAYAADLKDAEGQV
                     TGIYAERTGKSAEDVLAVLNAETWMNAETAVKEGWCDEVISPAAAESGQKETAAPGKE
                     EDGGGEEEDPDEEEKGGPPQNYTVLRRMMAAVGLAGKNSVEELEREVARLVAENERLA
                     AENDGFRGMQGQQARVMEAHEREFEQRVKEAVVREMAAMGVAPVGLPPAEGATEETGK
                     KEPAMTNEKLREMAAQDALEWIMGHPQEAARLAEQPGK"
     gene            3455..4435
                     /locus_tag="PK077_07625"
     CDS             3455..4435
                     /locus_tag="PK077_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24206.1"
                     /translation="MNKKTLMNIPRNAVMEGNDAAALNWTIVSQAAIATLEEELASIS
                     RFSLDVSGEFKTDGDSVKVEVIDGAGEALKNTEDWNKSELKTSSVSVTLNRYSRPAGL
                     SYKERKSGVQLANKVQTLVRTVAKAFWKDLMAAIADSGAEVVNIGPRAGFKPEMMADV
                     IWPSMTNGADAVYLDRMYYSRLIPTNALALNLADGAYSIPGGIHYVEGVNVLAGNAGV
                     GFATRPDALAVAVRLPNIDPKLNLETQVVESPKLGISLLLKCWPDQGTETVYISAELL
                     AGVAVGNKNHLRQLSGAAPETAAEGESVEDGGGEETVPTEGAGATEEEGA"
BASE COUNT         1015 a          979 c         1655 g          844 t
ORIGIN      
        1 tggaatgtga gtgaagtgaa gtcgcatccg gggctggagc tttatttgta cgtggaccgc
       61 accgccaaaa tggagcttta cgcggggcgc atccagaaag gggcggctcc ggccctgcat
      121 ttgccggaag atgcggatca ggatttgctg gccggattgt ccgggcagca gcttgagaag
      181 ccaaggggcg gcggcctggc gcaatggcgg aagctgccga atgaccatta tggagactgc
      241 gtaaaaatcg ggcaggtgtc ctggtgggtg cggcgcgggg atttttacgc ggaagaaatg
      301 aacgcgattg aagaaaggaa gcagaatgaa ggagtaccgg aagaatgacg tgctggagag
      361 gctgaaggcg gccgggtgcg aatgatagcg ggtttttgaa aaagctcctg aagggtatga
      421 atcccattgt acaggcttat gtggaaaatt atgatttgcc ggatttgcag ggaatgctgc
      481 gggaaaagct ggcgattctg gaagggcgca aggaaataac cggggcctcc acaggcggcg
      541 gaacgtccta caccgcgcag gagaccatga atttaaagga ccatatagcc tgcttgcagg
      601 aggcaatcac ggtcaagaag atggaggaag gggatttttc cggcctggcc gccgcggatg
      661 acggcgtgcg ggaagtgcgg tttgaccata ccataacacg cttttgacca tgggcaggaa
      721 cagaaggaag gtgtatgccg gggcgcgccg cggtcatggc gcgcgggtga agatgaaccg
      781 ggaaccggaa acggcgcgga gggagatgtg gggagggtat gcggccgcgt tgcagttcgg
      841 aggctccagc gtgttatact ggcctacgct ggacagccgg tttgaagtgg attcctggac
      901 gctggaccgg gtttggcgga atgcgcggaa tctggaagcg aattccgggc ttgccgggaa
      961 ggccgtggcg gatgtggtgg agttgctggg ctggctggtg ccccatgcct gcacggcgga
     1021 tgaagactgg aatcatgagg cggaccagat ttttatgaat cgggccgtga atccggaatt
     1081 gtttgacgcc cgcggagagc tgaatttttt tacggctcaa atatggagcg agcggcagcg
     1141 cgtgattgac ggcgatatgc tgacggtact gaccagcggg ccggatgacg gcggggcgtt
     1201 cgcgttttac gaggctccgc aagtgcaatc tccggctgat ggggggcagg cgtggaattg
     1261 cggcgtgatg cgggataaaa acgggaggac ggcggcttac gggctgcggc atccggacaa
     1321 gggggaggtg acggtgattc cggcccgtga tgctattttg taccggcaca acatgggcgg
     1381 agggaagccg cgcggcctgt ccgatttgca ccgcgctatc cggaatttgc atgatgaggc
     1441 ggatattgtg gggtatgtca agcagtctgc caagctggcc gcctccgttg ggctggtaga
     1501 aacgggggac gcggagaaac ggccgggcat ggggaccgtg ggcaaggtgt ccgtggggcc
     1561 ggacgggcgc agggtggagc aggtgttagg ggggcctact gtccaccagc ttccgcccgg
     1621 ccgggatttg aaggtgctga cggataacag gccgtctcct aatgtgatgg cgttgctgaa
     1681 gcatttgatg gatgaggtgg cttatggcat cgggctttcc cctgcgttgc tgtgggagcc
     1741 tgacaagctg ggaagcggcg gcatccggtt tgtgatgcag aagctgaagc gttggctgaa
     1801 aatcaggcat gcctacaggc aaatgtggtg cgtgcgggtg tggcgtttca tgctggcgcg
     1861 ggaaatggcc ctgggacggc tgcgcttgtg ccgggatccg cattgggtgc ggtgcctgtg
     1921 gacgcccatg agcgacatga ctattgacct ggggcgggaa gggaatctga tgattaacct
     1981 ggtggattcc gctctggcgg atcaggatgg ctggtgcctt gccaattacg gatgcacgtt
     2041 tgaggaaatc gtgaataaca agatacggaa tttgaagatg gctaaagaag cctgcgcccg
     2101 gaacggactg accctgcaag aggtgattcc gggagcgaac cgcggcgggg tagccgcggc
     2161 ggcggagaaa ccggaagatg aagagccggg aacgggcgac ggggaagagg aagatggctt
     2221 gcatccccat gaatagcaat tttgaaaaag ctcctgaagg gtacagaaca gtaataagtg
     2281 atgaataaga ttgtttttgc gcagatggcg gcacgtctgg aaggcggtgc cggtgaacag
     2341 aaaaaaacgg gcatgcttgc cttttcccgc atcatggagg cggaagagaa ggtaggggtg
     2401 gctaccattt ccggttatat cggttacggc aatgccacgg ttgacgaatt tacgaagcac
     2461 cttgaagagc tgaaggcgga ggggtgcacg aagtttgaag tcatcctgaa ttccatgggc
     2521 ggcaatttgt ttgaggcgtc cgggatttac gacattatca aggggtgcgg gatggaggtg
     2581 acggccaaaa tttacggggt agccgcttcc gccgcgacgc tgattgcctg tgcggcgggc
     2641 cgtgtgctga tttcggaaaa ttcccgttat atggtccacc gggcgcgcgg gtgcgcggtg
     2701 gggacggtgg aagagattga ggcttacgcg gcggatctga aggacgcgga agggcaagtg
     2761 acaggcattt acgcggagcg taccggaaag agcgcggaag acgtgctggc cgtgctgaac
     2821 gcggagacgt ggatgaacgc ggaaacggcc gtgaaggaag gctggtgtga cgaggttatt
     2881 tctccggccg ctgcggagtc cggccaaaag gaaacggccg cgccggggaa agaagaggac
     2941 ggcggcgggg aagaggaaga ccctgacgaa gaagagaagg gagggccgcc gcagaattac
     3001 acggtattgc gccgcatgat ggccgccgtg gggcttgccg ggaaaaacag cgtggaggaa
     3061 ctggaacggg aagttgcccg gctggtggcc gaaaacgaaa ggctggcggc ggaaaatgac
     3121 gggttccggg gcatgcaggg gcagcaggcg cgcgtgatgg aggcgcacga gcgggaattt
     3181 gagcaacgcg tgaaggaggc cgttgtgcgg gaaatggcgg ctatgggggt tgctccggta
     3241 gggctgccgc ctgcggaggg agccacggaa gagaccggaa agaaagaacc tgccatgacg
     3301 aacgaaaagc tgcgggagat ggccgcgcag gatgcgctgg aatggattat ggggcatccg
     3361 caggaggctg cgcggctggc ggagcagccg gggaaatagc atcttggccg ccatagatag
     3421 atttttacta acaaaacgca aacataaata aaatatgaac aaaaaaacat tgatgaacat
     3481 tccgcggaat gccgtgatgg aaggaaatga tgccgccgct ctgaactgga ccattgtttc
     3541 acaggcggct attgccaccc tagaggaaga attggcttca atcagccggt tttctctgga
     3601 cgtgtccggc gagttcaaga cggacggcga ttccgtcaag gtggaagtga ttgacggagc
     3661 cggggaggcg ttgaaaaata cggaagactg gaataaaagc gagctgaaaa ccagctccgt
     3721 ttccgtgacg ctgaaccgtt attcccggcc ggctggcctg tcctataagg aaaggaaaag
     3781 cggggtgcag cttgcgaata aagtgcaaac gcttgtgcgg acggtcgcca aagcgttttg
     3841 gaaggacctg atggccgcca tagccgattc cggggcggaa gtggtgaata ttggcccgcg
     3901 ggccggattc aagccggaaa tgatggcgga tgtgatttgg ccgtccatga ctaatggcgc
     3961 ggatgccgtt tatttggatc ggatgtatta ttccaggctg attcccacga atgcgcttgc
     4021 tcttaacctg gcggacgggg catattccat tccgggggga attcactacg tggaaggggt
     4081 gaacgtgctt gccgggaatg ccggggttgg ttttgcgacg cggccggacg cgctggccgt
     4141 tgccgtccgt cttccgaaca ttgatccgaa gctgaatttg gaaacgcagg tggtggaatc
     4201 tcctaagctg gggatttccc tgctgctgaa gtgctggcct gaccagggaa cggaaacggt
     4261 ttacatttcc gcggagcttt tggccggcgt ggcggtgggc aacaagaacc atttgcgaca
     4321 gctttccggc gcagctccgg agacggcggc ggaaggtgaa agcgttgagg acggcggcgg
     4381 ggaagaaaca gtgccgactg aaggtgctgg ggcgactgaa gaagaagggg cctgacgggt
     4441 ttttggcgga atcatgggat aaaagagagc aaaggaccgg cgcgcggggt gtc
//
LOCUS       DAOMSU010000172         4492 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1821_length_4492_cov_2.330854, whole genome shotgun sequence.
ACCESSION   DAOMSU010000172 DAOMSU010000000
VERSION     DAOMSU010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1821_length_4492_cov_2.330854"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1719
                     /locus_tag="PK077_07630"
     CDS             163..1719
                     /locus_tag="PK077_07630"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HRN24207.1"
                     /translation="MSDQIYFFDTTLRDGEQCPGASMNLREKLEVARQMERLGMDVIE
                     AGFPCISDGDFEAVHTIAREIKKCRIAGLARCVKADIEAAAKALEPAGERARIHIFLA
                     TSKLHMQFKLKKAESEILRMAAEGVSYAKQFVQDVEFSPEDASRTDLDFLTKVVETVI
                     DAGATTVNIPDTVGYTTPDEFYRIIRHLKENVPNIGKAVISVHCHNDLGLAVANSLAA
                     VRAGARQVEGTINGIGERAGNVALEEVIMALRTRAGQFGDVTDNINTKEIVRTSRIVA
                     RMSGMQVQRSKAIVGENAFAHSSGIHQHGILNCRETYEVMDPQAVGWGATELPLTKHS
                     GRAAVKSRLEQLGHVLTEEEVNTVFERFKKVGDSKKFVYDDDLSAIVDDSLHASTGLW
                     ELDFLQFVAGSHARPTATVGLIKEGKKFEDSSTGNGAVDAVIKAIERITRRKGTLKGY
                     SVNAASEGKDALGEVTVHVDFGQPKPIVGKGASTDVIEASARAYLNAVNRSIRMENMP
                     QPNNLDAGTI"
     gene            1841..2755
                     /locus_tag="PK077_07635"
     CDS             1841..2755
                     /locus_tag="PK077_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24208.1"
                     /translation="MKATLLLLCLACSALAAPPAKKQPAPVEPLPALEKNTIAIDGHP
                     ESVAADAEGNIYFTCIGSRLTPTEKDKDGYLGVIPHGSTEPKKITDVDTLDAPKGLLY
                     QDGFLYCTDVDMVFKINAKTGAIEGYVDLSPSRMKFLNDLAFINGRLMVSSTDTNQIF
                     YVDTNTSSYGELVTKQPIYKPNGLAWDPERKVIYLCEYATDEKGKPSGRLLSINPVSR
                     EVTELSKERGQYDGLVYRDNALYYSDWSKDKKPEAVRRLDLKTGRSAPVATGPVEGAA
                     DFILYDSMMVVPGMTEKKIHIMPIGGKK"
     gene            2875..3330
                     /locus_tag="PK077_07640"
     CDS             2875..3330
                     /locus_tag="PK077_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRN24209.1"
                     /translation="MNTISDQIMEGMKTAMKAKDSVTLNTLRALKTALTNTAIAKGGL
                     GTRLEEAEELAVVRKQIKQREDSAEQFRAAGRPELAEKEEAEITVLKQFMPAELTAEE
                     TAAILETVMKETGASTKKDMGQVMKLMQERTAGRVNGKELARMVSARLS"
     gene            3327..4022
                     /gene="ispD"
                     /locus_tag="PK077_07645"
     CDS             3327..4022
                     /gene="ispD"
                     /locus_tag="PK077_07645"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HRN24210.1"
                     /translation="MKCCAAIIVAAGSSRRAGFDKLLAPLHGVRVLERSIRAFANCRE
                     ITEIVVVCPEERFHAINGANLETEIPVTRVDGGAERHESVQNGLAALLYTPEFVAVHD
                     GARPLITVEQISRCIQTAREYGASASAHPVTDTLKRADKERFTLPEQVERDGLWCMET
                     PQVFQYPLLLDAYVEVTERNIQVTDEVTALQLIGHPTRLVHNHEPNPKITWPEDISRA
                     EMLMELKHLRNYS"
     gene            4019..>4492
                     /locus_tag="PK077_07650"
     CDS             4019..>4492
                     /locus_tag="PK077_07650"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRN24211.1"
                     /translation="MTGLLCKNPRIVLILGTGYLGKALAESLRKAGHTALTADIDPQK
                     AIYEADVADQASMQGLAARIPSPQIIVMCASTRGGSEEAYRSLYIRGTQNTLEAFSGT
                     PVIFCSSTAVYGITDGRWITEEHNVYPSSGKNGLLIQAEQAVLAAGGTVVRLGALY"
BASE COUNT         1098 a         1350 c         1223 g          821 t
ORIGIN      
        1 ccatcctaca aagctcctcc ttttaggcaa agggcggttc cgtcattctt cgctccggcg
       61 gttctcttcc gccgcccgga cgctctgtga aagcatgcag cgcgtagggc ttgcgttact
      121 ggagcaaaac attatcatgc cactgcttaa catcattaac ctatgtccga tcaaatctac
      181 ttttttgaca ctaccctgcg cgatggagaa caatgccccg gagcatccat gaacctacgc
      241 gaaaagctgg aagtggcgcg tcaaatggaa cggctgggca tggacgtgat tgaagccggc
      301 tttccctgca tctccgacgg cgactttgaa gccgtgcaca ccatcgcccg cgaaatcaaa
      361 aaatgccgca tcgctggtct ggcccgctgc gtaaaggcgg acattgaagc ggcggccaaa
      421 gccctggaac ccgccgggga acgtgcccgc atccacattt tcctggccac cagcaagctg
      481 cacatgcagt tcaaactgaa aaaagcggaa tccgaaatcc tgcgcatggc cgcggaaggc
      541 gtatcctatg ccaaacaatt tgtccaggac gtggaatttt ccccggagga cgcttcccgc
      601 acagacctgg acttcctgac caaagtcgtg gaaacagtca tcgacgccgg cgccactacc
      661 gtcaacattc cggataccgt gggctacacc acaccggacg agttctaccg catcatccgc
      721 cacctgaagg aaaatgtgcc gaacatcggc aaagccgtca tctccgtgca ttgccacaat
      781 gaccttggcc tggccgtagc caactccctg gcagccgtcc gcgccggagc ccgccaggtg
      841 gaaggaacca ttaacggcat cggcgaacgc gccggaaacg tggctctgga agaggtcatc
      901 atggccctgc gcacccgcgc cggacaattc ggagacgtca cggacaacat caacacgaag
      961 gaaatcgtcc gcacttcccg catcgtggcc cgcatgagcg gcatgcaggt gcagcggtcc
     1021 aaagccatcg tgggggaaaa cgctttcgcc cactcttcag gcatccacca gcacggcatc
     1081 ctcaactgcc gggaaaccta tgaagtcatg gacccgcagg cggtcggctg gggagccacg
     1141 gaactcccgc tcaccaagca ttccggacga gctgccgtca aatccaggct ggaacagctg
     1201 ggccatgtcc ttacggaaga ggaagtcaac accgtctttg aacgcttcaa gaaagtgggc
     1261 gactccaaaa aattcgttta tgatgatgat ctttccgcca ttgtggacga ctccctgcat
     1321 gcctccaccg gcctatggga actggacttc ctgcaattcg tggcaggcag ccacgcgcgg
     1381 cccacggcta cggtgggact gatcaaggaa ggcaagaaat ttgaagacag ctccacgggc
     1441 aatggtgccg tggacgccgt tatcaaagcc attgagcgca tcaccaggcg caaaggcacg
     1501 ctgaaaggct acagcgtcaa cgccgcttcc gaagggaagg acgctctggg agaagtcaca
     1561 gtgcatgtgg acttcggcca gcccaagccc attgtgggca agggagcctc cacagacgtc
     1621 attgaagctt ccgcccgggc ctacctgaac gcggtcaacc gttctatccg catggaaaac
     1681 atgccccagc ccaataatct ggatgcagga accatctaac acgggaagcg ccccgtttgc
     1741 ccgaacgggg tgcaactccg cccatcagcc tgccagcctc tgcggggctt gcttttccat
     1801 tgctcccatg ccataaggtt caaaactctt acaccacccc atgaaagcga cattactcct
     1861 tctttgcctg gcgtgcagcg cgctggccgc accgccggca aaaaaacagc cagcccccgt
     1921 tgaaccgctg cccgccctgg aaaaaaacac cattgccatt gacggccatc cggaaagcgt
     1981 tgcagcggat gccgagggca atatctattt cacctgcatc ggctccaggc tcacgcctac
     2041 ggaaaaagac aaggacggct acctgggcgt cattccgcac ggttccacag agccgaaaaa
     2101 aatcacggac gtagataccc tggacgcgcc gaaggggttg ctgtatcagg acggcttcct
     2161 ttactgcacg gacgtggaca tggtattcaa aataaacgcc aagaccggtg ctattgaagg
     2221 atacgtggac ttgtccccat cccgcatgaa attcctcaat gacctggcgt tcatcaatgg
     2281 caggctcatg gtttcctcca cggataccaa ccagatcttc tatgtggaca cgaataccag
     2341 ctcttatgga gaactggtga ccaaacagcc catctacaag ccgaacggcc tggcctggga
     2401 cccggaaaga aaggtcattt acctctgcga atacgcaacg gatgaaaagg gcaaacccag
     2461 cggacgcctg ctctccatca accccgtttc ccgggaggtc acggaacttt ccaaggaacg
     2521 gggacagtat gacggactgg tttaccgtga caacgccctt tactacagtg actggtccaa
     2581 ggataaaaaa ccggaagctg tccgcaggct ggatttgaag acggggcgtt ctgccccggt
     2641 agccaccggc cctgttgaag gggcggcgga ctttatcctg tatgactcca tgatggtggt
     2701 tccgggcatg acggaaaaga aaatccacat catgcccatt ggcgggaaaa aataaacacg
     2761 gctgttccgc ttttattccc cttatccggt ccgccggcgg cattccctgc gctggcggcc
     2821 tttttcaatt tacggcttga agtacaccta gggatgcgct tagttaatgg agatatgaac
     2881 acgatttccg accaaatcat ggaggggatg aaaaccgcca tgaaagcaaa agactccgtt
     2941 accctcaata ccctgcgcgc cctcaagacg gctctcacga acacggccat cgccaaggga
     3001 ggcttgggaa cccgactgga agaagcggaa gaactggccg tggtccgcaa gcaaatcaag
     3061 cagcgtgagg attctgcgga acaattccgc gccgccggac gcccggaact ggcggaaaag
     3121 gaagaggcgg aaatcaccgt tctcaagcaa ttcatgcccg cggaactcac agcagaagaa
     3181 acggcggcca ttctggaaac cgtgatgaag gaaacgggag cctctaccaa aaaagacatg
     3241 gggcaggtca tgaaactcat gcaggaacgc acggccggca gagtcaacgg caaggagttg
     3301 gcccgcatgg tgtcagccag actgtcatga aatgctgcgc cgccatcatc gttgcggccg
     3361 ggtcctcccg gcgagccgga tttgacaagc tgctggcccc cctgcacggc gtcagggtgc
     3421 tggaacgcag catccgcgcc tttgccaact gccgggaaat cacggaaatc gtagtcgttt
     3481 gcccggagga acgttttcat gccattaacg gcgcaaacct ggaaacggaa atacccgtta
     3541 cccgtgtgga cggaggggcg gaacggcatg aatccgtgca gaacggactg gcagccctgc
     3601 tttatacccc ggaattcgtg gccgtgcacg atggagcccg cccgctcatt acggtggagc
     3661 aaatttcccg ttgcattcaa accgccaggg aatatggggc gtccgcttcc gcacatcccg
     3721 taacggatac cctgaagcgt gcggataaag aacgctttac gcttccggaa caggtggaac
     3781 gggacgggct gtggtgcatg gaaaccccgc aggtctttca atatcctctt ctcctggacg
     3841 cctatgtgga agtcacggaa cggaatattc aggtaacgga tgaagtaacc gccctgcaac
     3901 tcatcggcca tcctacgcgc ctggtgcata atcatgagcc caatcccaaa atcacatggc
     3961 cggaagacat ttcccgcgct gaaatgctaa tggaactcaa gcacctccgc aattactcat
     4021 gaccggcctt ctgtgtaaga atccccgcat cgttctcatt ctgggaacag ggtatctggg
     4081 aaaagccctg gcggaaagcc tgcggaaagc gggacatacg gccctgacgg cggatattga
     4141 cccgcaaaaa gccatttacg aagcagacgt tgcagaccag gcctccatgc aggggctggc
     4201 cgcccgcatc ccctcccccc agatcatcgt catgtgcgcc agcacgaggg gaggctcaga
     4261 ggaagcctac cgcagcctct acatccgcgg aacgcagaac acgctggaag ccttttccgg
     4321 aacgccggtc atcttctgtt ccagcacagc cgtctatgga atcacggacg gacgctggat
     4381 tacggaggaa cacaacgttt atccctcctc tggaaaaaac gggctgctta ttcaggcgga
     4441 acaggcggtt ttggcagcgg ggggcacggt cgtccggctg ggagccctgt ac
//
LOCUS       DAOMSU010000173         4488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1823_length_4488_cov_2.783668, whole genome shotgun sequence.
ACCESSION   DAOMSU010000173 DAOMSU010000000
VERSION     DAOMSU010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1823_length_4488_cov_2.783668"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PK077_07655"
     CDS             <1..1067
                     /locus_tag="PK077_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRN24212.1"
                     /translation="KPGQKNVIAVKADNSNDASYPPGKPQEGLDFSYFGGIYRDVYLI
                     STGPVHITDPNEAGTVAGGGVFFRTESLDPRTRKGKVGVKVQVANQTDKEQKVRVQAV
                     MTDPKGVDPAGETVPLTIPPHSTGEVDIPLVLSNVKPWSPDNPDLYTLSVEVWDAAGG
                     DHSKDPAHLLDNRSIRVGVRTVEITEKGLVLNGTLFPEKLIGGNRHQDFARLGNAVPN
                     NLQWQDAVKLRKAGMRVIRSAHYPQDPAFMDACDRLGLFVIVATPGWQFWGSGPFADR
                     VYDDIRQMVRRDRNHPSVMMWEPILNETHYPADFAKKARDLVHEEYPYKGCYTACDAV
                     AQGSQHYEVLYAHPVTGDKH"
     gene            1125..2249
                     /locus_tag="PK077_07660"
     CDS             1125..2249
                     /locus_tag="PK077_07660"
                     /inference="COORDINATES: protein motif:HMM:NF037850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24213.1"
                     /translation="MDTWSAHNSTSRVARHWGEVPMIVQALHYLKTSFPYTTYDTLNS
                     APAYHFGGCLWHPFDHQRGYHPDPFYGGILDAFRQPKTSYYAFMSQRPQKTRNGLGSG
                     PMVHIANECTPFSPEDVTVFSNCDSVRLSVNGGPPVEKKVASCPGGLKRVPVVFPKAW
                     DFMENKKLARGGKEGAVKLVAEGLIGGKVVARHEVRPARRAEKIRLTLDREDGVDFYA
                     NGSDVFAVVAEVTDGRGTVKRLNDEEIVFSVEGPAELLTDSPDGTLTQPVKWGSAPAL
                     VRLGAKPGTVTVRASVKHPGSQKPVSGVIKFDTKAPGLKMLFTEDAVGCGKKAAGTPS
                     SAAASVSEREKSLQMELEKVRHELNSLRNEKVSAQQTHFE"
     gene            2263..2736
                     /locus_tag="PK077_07665"
     CDS             2263..2736
                     /locus_tag="PK077_07665"
                     /inference="COORDINATES: protein motif:HMM:NF018942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HRN24214.1"
                     /translation="MMLREKIVRFCLLLLRLGMGGFFLFVAVRKLLHLDQLQETIDRF
                     AIFPEAWGHPLACLGVSCEIVVGLGLLFRRTCAGAALLGSALTFAFVALFVQGWARGL
                     SLSCNCIGVERAVTSYPFEVAWRLVLFSLMLILLWDSCRKGKTYFNVTRLDFSEM"
     assembly_gap    2818..2863
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3010..4089)
                     /locus_tag="PK077_07670"
     CDS             complement(3010..4089)
                     /locus_tag="PK077_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRN24215.1"
                     /translation="MKISDDSLEFLTELLETPSPSGFEIDAQRIWADELRKYTEDVQC
                     DTYGNTWAVFHADAEEAPTLMIEAHADEIGFMIRHITKDGFLYVERVGGTDTAIARGR
                     RVRFLGSQGEVMGVTGNTAIHLREPGEKEPKIWEIYVDVGASSDKEVAELGLRVGHVG
                     VYCDGPMLMNENKLVCRALDNRLSGFILSEIARKLCKLKKPVAWNVVFVNAVQEEVGC
                     IGAGMITHRLRPDAAICIDVTHATDSPGLDKGKFGDIKLGGGPAVIHGTANHPNLVAR
                     LEIVADKNKIPLQHEAAGRRTGTDTDSIYISRDGVASALVSVPLRYMHSPVETASLTD
                     VENTIKLLLELVKSLTPEDSFGHRL"
     gene            complement(4233..>4488)
                     /locus_tag="PK077_07675"
     CDS             complement(4233..>4488)
                     /locus_tag="PK077_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24216.1"
                     /translation="VRDVEKAWECGIFSVNLVPEPVTAFELVEQCFPALVNRLPVAKE
                     TDPYGSARTSVYSTQYHDPDTGYIMDKQDVLAGLSPDKQS"
BASE COUNT          883 a         1228 c         1343 g          988 t
ORIGIN      
        1 tgaaaccggg acaaaagaac gtcatcgccg tgaaggcgga caattccaac gacgcttcct
       61 atccccccgg caaaccccag gaaggcctgg atttttccta tttcggcggt atttaccggg
      121 atgtgtacct catttccacc ggtcccgtac atattacgga tccgaacgag gccggcaccg
      181 tggccggcgg gggagttttt ttccggacgg aatctcttga tccccgcacc cgcaagggca
      241 aggtaggggt gaaggttcag gtggccaacc agacggataa ggaacagaag gtgcgcgtgc
      301 aggcggtgat gacggacccc aagggggtgg atcctgccgg ggagacggtt ccgctgacca
      361 tcccacccca ttccacgggt gaagtggata tccctcttgt gctttccaac gtgaaaccgt
      421 ggtctccgga caatcccgat ttgtatacgc tgagcgtgga agtatgggac gctgctggag
      481 gggaccattc caaagatccg gcccatttgc tggataaccg ttccataagg gtgggtgtcc
      541 ggacggtgga aattacggaa aaggggctgg tgctcaatgg aaccctgttt cctgaaaagc
      601 tgattggcgg caacaggcac caggattttg cccggcttgg gaatgcagtg cccaacaacc
      661 tgcaatggca ggatgccgtg aaattgagaa aggcaggcat gcgcgtgatc cggagcgccc
      721 attatccgca ggatccggct ttcatggacg cctgcgaccg tctgggcctt ttcgtcatcg
      781 tcgccacgcc aggctggcag ttctggggca gcggcccttt tgcagaccgg gtgtatgacg
      841 atatccgcca gatggtgcgc cgggacagga accatccctc cgtgatgatg tgggaaccta
      901 ttttgaatga aactcattac ccggcggatt tcgccaaaaa ggcccgcgac ctggtgcatg
      961 aggaatatcc gtacaaggga tgctacaccg cttgcgacgc ggtggctcag ggcagccagc
     1021 attatgaagt tctgtatgcc cacccggtta cgggggacaa acactagtcc atcaaggaac
     1081 ggaagaacag caaaccgtat ttcacccgtg aattcggtga taacgtggat acctggtccg
     1141 cccacaattc cacctcccgt gtagcccgtc actggggtga agttcccatg atagtgcagg
     1201 cccttcatta cctcaagacc tcgttcccat ataccacata cgatacgctg aattccgctc
     1261 ctgcctacca tttcggcggc tgtctgtggc atccttttga ccatcagcgc ggctaccatc
     1321 cggatccctt ctacggcggt attctggatg ctttccggca gcccaagacc tcctattacg
     1381 catttatgtc ccagcgtccc cagaagacgc gcaacgggtt gggttccggc cctatggtgc
     1441 acattgccaa cgaatgcact cccttttctc cggaagacgt gacggtgttt tccaattgtg
     1501 attccgttcg cctgagtgtc aacggagggc ctccggtgga aaagaaggtg gcatcctgtc
     1561 ccggaggcct caagcgcgtg cccgtcgtgt tccctaaagc ctgggatttc atggaaaaca
     1621 agaagcttgc ccgaggaggc aaggagggag cggtgaaact ggtggcggaa ggtctgatag
     1681 gcggcaaggt ggtggccagg catgaagtcc gcccggcccg gagagcggaa aaaatccgtt
     1741 tgactctgga tcgggaagat ggagtggatt tttacgccaa cggttccgac gtgtttgccg
     1801 tggtggcgga ggttacggat ggccgcggaa cagtgaagcg cctgaatgat gaggagattg
     1861 tcttttccgt ggaaggtcct gccgagctgc tgacggattc cccggacggc acgctgaccc
     1921 agcccgttaa atggggttcc gctccggctc tggtgcgtct gggcgccaag cccggcacgg
     1981 tgacggtgag ggcctccgtg aagcatcccg gctcccaaaa gcctgtttcc ggcgttataa
     2041 agtttgacac gaaggctccc gggttgaaaa tgctctttac ggaagacgcc gtaggctgcg
     2101 ggaagaaagc tgccgggacg ccgtcttccg ccgcggcttc tgtctccgaa cgggagaaat
     2161 cccttcagat ggagctggaa aaggtgcgcc atgaactgaa cagtttgcgc aatgaaaaag
     2221 tgagcgccca gcaaacccac ttcgaataag cggaatacgc caatgatgct aagggaaaag
     2281 atcgtccgtt tttgcctgct cctgctccgg ctggggatgg gaggcttttt cctgttcgtc
     2341 gcggtgcgca agctgcttca tctggaccag cttcaggaga cgatcgaccg tttcgccatt
     2401 ttcccggagg cctgggggca tcccctggcc tgcctggggg tgtcgtgtga aattgtggtg
     2461 gggctgggac tcctgttccg cagaacctgc gccggggccg cgctgctggg cagcgcgctc
     2521 acgttcgcgt tcgtggcgtt gttcgtccag ggctgggcca gggggctttc cctctcgtgc
     2581 aactgcatcg gcgtggagag ggcggtaacc agttatccct ttgaagtggc ctggaggctg
     2641 gtcctctttt ccctcatgct gattcttctg tgggattcct gccggaaggg aaaaacgtat
     2701 tttaatgtga cccggctgga ttttagtgaa atgtgaccgg aaggaaagac tcctgccgtt
     2761 ttccggttcg ggataatttc cgggaaaatg ttccgggaga ggaggccggg aaccatcnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngccaatg gttacgccct
     2881 gctccatata agggggcttc catgcagcca catggaagca ggccggggaa atggctgttt
     2941 ttgcgcgcct ttccccggca ggacggttca tgtccttatg ttgtcaacga tgccgccgta
     3001 tactccgtat tacagcctgt ggccgaaaga gtcttccggc gtcagggatt tgaccagttc
     3061 cagcagcagc ttgattgtat tttccacatc tgtcagagag gccgtttcca ccggggaatg
     3121 catatagcgc agggggacgg acaccagcgc ggaggctacg ccgtcgcggg agatgtagat
     3181 actgtccgta tccgttccgg tgcggcgtcc ggcggcttca tgctggagcg gtattttgtt
     3241 cttgtccgcc acgatttcca gacgggccac cagattggga tgattggctg tgccgtggat
     3301 gaccgcaggg ccgcctccaa gcttgatatc gccaaatttg cccttgtcca gtccgggcga
     3361 gtccgtggca tgagtcacgt caatgcagat ggccgcgtcc gggcgcaggc ggtgggtaat
     3421 cattcccgcg ccaatgcagc ccacttcttc ctgcacggcg ttgacgaaca ccacgttcca
     3481 ggcgacgggc tttttcagct tgcacagctt ccgggctatt tccgacagga tgaagccgct
     3541 cagccggttg tccagagccc ggcataccag cttgttttca ttcatcagca tggggccgtc
     3601 gcagtaaacg ccaacgtggc ccactcgcag accgagttcc gctacttcct tgtcagagga
     3661 ggcgcctacg tctacgtaaa tttcccagat tttgggttcc ttttctccgg gttccctcaa
     3721 gtggatggcc gtgtttccgg tcacccccat cacttcgccc tgggaaccaa ggaagcgcac
     3781 gcggcgcccc cgtgcgatgg ccgtatccgt accgcccacg cgttccacat acaggaagcc
     3841 gtccttggtg atatggcgga tcatgaagcc gatttcatcc gcatgggctt caatcatcaa
     3901 tgtgggagct tcttccgcgt ccgcgtggaa gacggcccag gtatttccgt aggtgtcgca
     3961 ctggacatct tccgtatatt tgcgcagttc gtccgcccag atgcgctggg cgtctatttc
     4021 aaagccggaa gggcttgggg tttccagaag ttccgttaaa aattcgaggc tgtcgtcact
     4081 gatcttcatg gcttttgtat agcaccgttc cggcatgcgc tcaacaggaa agcacggctc
     4141 ctgcagtgga gctctttttg agggcgggga ataaaaagcc ccgactgcgc aggaagcaga
     4201 cggggcctgg aaaaagtttt tttgaccggg ggctaggact gtttgtccgg ggaaagcccc
     4261 gccaggacgt cctgcttgtc catgatataa ccggtgtccg gatcgtggta ttgagtggag
     4321 taaacggatg ttctggcgct tccgtacggg tccgtttcct tcgcgacggg gaggcggttg
     4381 accagggcag ggaagcactg ctccaccagt tcaaaggcag tgacgggttc cggcaccaga
     4441 ttcacggaaa agatgccgca ttcccaggct ttttccacgt ccctgacc
//
LOCUS       DAOMSU010000174         4428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1851_length_4428_cov_2.586554, whole genome shotgun sequence.
ACCESSION   DAOMSU010000174 DAOMSU010000000
VERSION     DAOMSU010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1851_length_4428_cov_2.586554"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3058
                     /gene="secA"
                     /locus_tag="PK077_07680"
     CDS             <1..3058
                     /gene="secA"
                     /locus_tag="PK077_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRN24217.1"
                     /translation="VEATPASIEEGKAAWNNITPQFDKLRERYLNQILPEAFAAVKHG
                     ARLLCGEERDICGQKQVWDMVHFDVQLLGGIALHRGYIAEMATGEGKTLVATLPVYLN
                     ALTGMGVHVVTVNDYLARRDSEWMGMLFQFLGLTVGCIQSMMPSQLRREQYACDITYG
                     TNAEFGFDYLRDNGMATSKSEQVQRGHYFSIVDEVDSILIDEARTPLIISGPAVVTRE
                     QQYDTLRPAIERVVKAQTDLCNELMAQALKAQEEGRTEEVGRCLFKVKMGQPRHRAFL
                     RAMQDPELRRIVEKYELTLYQDTRKKELYKLKEEMFFTVDEKTHDADLMEKGREVISP
                     GHPEDFVLPDLGTAFAEMDEDPRLTEKDKLRRKNELTKQLDETGARLHTTSQLLKAYC
                     IYEKDVEYVVKEGKVIIIDQNTGREMPGRRWSDGLHQAVEAKEGVEVERENQTYATIT
                     IQNYFRLYKKLAGMTGTAETEAAEFHDIYKLDVLPIPTNRPCIRKDQNDLIFKSRREK
                     FNAVVNKIQELHDKGQPILIGTASVDASETLSRMLKRAKIPHEVLNAKNHQREAEIVA
                     QAGKRGAVTVSTNMAGRGTDIKLGEGVADLGGLFVLGTERHESRRIDRQLRGRCSRQG
                     DPGASQFFISFEDDLMRNFGAAERMTKMMERLGVADGEALEHSFLNKSVESAQKRVEQ
                     RNYMWRKHVLDYDDVMNKQREIVYGYRNEVLSTENPREMIYDILEEVIATRAHEFLDP
                     DAEGVTHPDELLAWMNASFPLGLTAEAAKLEERPIDDTIAFLIDKVKATYEDKASRER
                     PEYLDHMERQIILGAIDKMWQEHLYNMDSLREGVRLRAQGQKDPLVEYKSEAYDLFIT
                     LMESIKSEAISNLFKSTTNLDAFEDFLASLPQFESSDENQEGGTSLPEIGFDGMPSDL
                     LSALREQVSRAREQQSARQAEPEQAPAVISDATTIGEGYKPAVAEPKLVMPKRKVSVV
                     LRKEEASQAPAETPAPGDGEEIAVTLDSQDFAETMDNRDSADTRTF"
     gene            3135..4235
                     /locus_tag="PK077_07685"
     CDS             3135..4235
                     /locus_tag="PK077_07685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN24218.1"
                     /translation="MATLPPNKSANVRSVLEYVPYFRDKIFAVHVERPLVDSEELVDA
                     LLDLDVLQEIGVRPVLIVEGADASALYEHTRVCEMRSALVEAPLKGGQLVRERVREIL
                     GRHQIPVVASGRSGSFDPESVHMAFSLGASKYIALLNDHKVPSLDGRPIAAILESEVA
                     ELAGNVTHRELLDQAAEACRAGIPRVHLLDGKMRGVLVEELFSEEGVGTMVHTDSYRE
                     IRPLKEEDIPELLSMIARSVVDSKLVNRNYEDIAARMDSYYVLTLDDSIVGCVAVYPY
                     PEHHSAELGCLYIKHRHEGRGYGRALCEFAKRKAEEMGMEFIFALSQSAVHYFRDRMH
                     YAEFSRDSLPPERLRTLELSGRKSGVFGLRLK"
     gene            complement(4240..>4428)
                     /locus_tag="PK077_07690"
     CDS             complement(4240..>4428)
                     /locus_tag="PK077_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24219.1"
                     /translation="IKKSLFLAMRRRRQGGMGFRCCGVSSEQLNQKGPEKPQPGTVAR
                     PFGPVNSAHKQERGKIGQ"
BASE COUNT         1058 a         1365 c         1222 g          783 t
ORIGIN      
        1 ggtggaagcc actcccgcct ccattgaaga agggaaagcc gcctggaaca acatcacgcc
       61 tcagtttgac aagctgcgcg aacgttatct gaaccagata ctgccggagg cctttgccgc
      121 cgtcaaacac ggcgcccgcc tgctctgcgg ggaagaacgc gatatctgcg gtcaaaaaca
      181 ggtttgggac atggtccact ttgacgtgca gctgctgggc ggcattgccc tgcaccgcgg
      241 ctacattgcg gaaatggcca cgggggaagg gaaaacgctt gtcgccaccc tccccgttta
      301 cctgaatgcc ctgaccggca tgggtgtgca tgtggtgacc gtgaacgatt atctggcgcg
      361 ccgcgactcg gaatggatgg gcatgctctt ccagttcctg gggctgactg tcggctgcat
      421 ccagagcatg atgccctccc agctccgccg ggagcaatac gcctgcgaca tcacttacgg
      481 caccaacgcc gaattcggct tcgactacct gcgcgacaat ggcatggcaa cctccaagtc
      541 cgaacaggtg cagcgcgggc actacttctc cattgtggac gaagtggact ccatccttat
      601 tgacgaagcc cgcacccccc tcatcatttc cggccctgcg gtcgtcaccc gggaacagca
      661 gtacgatacc ctgcgccccg ccattgaacg cgtggtgaag gcccagacgg acctttgcaa
      721 cgaactgatg gcccaggccc tgaaagccca ggaagaaggc cgcacggaag aagtgggccg
      781 ctgcctgttc aaagtaaaaa tgggccagcc ccgccaccgc gccttcctgc gcgccatgca
      841 ggatcccgag ctgcgccgca tcgtggaaaa atatgaactg accctttacc aggatacccg
      901 caagaaggaa ctctacaagc tgaaggagga aatgttcttc accgtggatg aaaaaaccca
      961 cgacgccgac ctgatggaaa aaggccggga agtcatctcc cccggccatc cggaagattt
     1021 cgtgctgccg gatctgggaa ccgcctttgc ggaaatggac gaagatccac gcctgacgga
     1081 aaaagacaaa ctgcgccgca aaaatgaact gaccaaacag cttgacgaaa cgggagcgcg
     1141 cctgcacacc acctcccagc tgctgaaggc ctactgcatt tacgaaaaag acgtggaata
     1201 cgtcgtcaag gaaggcaagg tcatcatcat cgaccagaat acgggccgtg aaatgccggg
     1261 acgccgctgg agcgacggtc tgcaccaggc ggtggaagcc aaggaaggcg tggaagtgga
     1321 gcgggaaaac cagacgtacg cgaccatcac catccagaac tacttccgcc tgtacaaaaa
     1381 actggcgggc atgaccggca cggcggaaac ggaagccgcg gaattccatg atatttacaa
     1441 gctggacgtt cttcccatcc cgaccaaccg cccctgcatc cgcaaggatc agaatgacct
     1501 catcttcaaa tcccgccgtg aaaaattcaa tgcggtggtc aacaaaatcc aggaacttca
     1561 cgacaaaggc cagcccatcc tgatcggcac ggccagcgtg gacgcctccg aaacgctctc
     1621 ccgcatgctc aagagggcca aaatccccca tgaagttctg aacgcaaaaa accaccagcg
     1681 cgaagcggaa atcgtggcgc aggccggcaa gcgcggagcc gtcaccgtct ccaccaacat
     1741 ggcgggccgc ggcacggaca tcaagctggg cgaaggcgta gccgacctcg gcggcctctt
     1801 cgtcctgggt acggaacgcc atgaatcccg ccgcattgac cgtcagctgc gcggccgctg
     1861 ctcccgccag ggggaccccg gcgcctcaca gttcttcatc tcctttgaag acgacctgat
     1921 gcgcaacttc ggcgcggcgg aacgcatgac caagatgatg gaacgcctgg gcgtagccga
     1981 cggtgaagcg ctggaacaca gtttcctgaa caaatccgtg gaatccgccc agaaacgggt
     2041 ggaacagcgc aactacatgt ggcgcaagca cgtgctggac tatgatgacg taatgaacaa
     2101 gcagcgcgaa atcgtttacg gataccggaa tgaagtgctt tccacggaaa acccgcgcga
     2161 aatgatttac gatattctgg aagaagtaat cgccacgcgc gcccatgaat tcctggaccc
     2221 ggatgcggaa ggcgtcaccc acccggacga attgctcgcc tggatgaacg catccttccc
     2281 gctgggactc acggcggagg ccgccaagct ggaagaacgg cccatagacg acaccattgc
     2341 cttcctcatt gacaaggtca aggccactta tgaagacaag gcttcccgcg aacgcccgga
     2401 atacctagac cacatggaac gccagatcat cctgggggcc attgacaaaa tgtggcagga
     2461 acacctgtac aacatggact ccctacggga aggcgtgcgc ctccgcgccc aggggcagaa
     2521 agaccccctg gtggaataca aatcggaagc ttacgatctc ttcatcaccc tgatggaaag
     2581 catcaagagc gaagccatca gcaacctctt caagagcacc accaacctgg acgcctttga
     2641 ggacttcctg gccagcctac cccagtttga atcttccgac gaaaatcagg aaggcggaac
     2701 cagcctgccg gaaatcggct ttgacggcat gccttcggac ctgctctccg ccctgaggga
     2761 acaagtctcc cgggcacgtg aacaacaatc cgcccggcag gcggaaccgg aacaggcccc
     2821 ggccgtcatt tccgacgcca ccaccattgg ggaaggttac aaaccagccg tggcggaacc
     2881 caaactggtc atgcccaagc gcaaggtcag cgtcgtgctc cgcaaggaag aagcctccca
     2941 ggcgccggcg gaaacgccgg cccccggaga cggagaagaa attgccgtca ctctggactc
     3001 ccaggacttt gcggaaacca tggacaaccg ggattccgcg gatacccgta cattctaaaa
     3061 acgcagcgca tcacttacag gccgtgggaa aatggaatta tccttttccc acggccctaa
     3121 gcttccggtc agccttggct acgctccctc ccaacaaatc cgccaatgtc cgttccgtgc
     3181 tggagtatgt tccgtacttc agggataaaa tctttgccgt gcatgtggaa cgtcctctgg
     3241 tggattccga ggaattggtg gacgccctgc tggacctgga tgtactgcag gaaataggcg
     3301 tcaggcccgt tctgattgtg gaaggggcgg acgcttccgc gctgtacgaa cacacgcgcg
     3361 tctgtgaaat gcgctccgcc ctggtggaag cgccgttaaa aggcggccag cttgtccggg
     3421 aaagagtccg ggaaatcctg gggcgtcacc aaattcccgt ggtggcttcc ggccgttccg
     3481 gctcctttga cccggaatcc gtccatatgg ctttcagcct gggggcctcc aaatacatcg
     3541 ccctgctcaa tgaccacaag gttccgtccc tggacggacg ccccattgcc gccattctgg
     3601 aatcggaagt agcggagctg gcaggaaacg taacccaccg cgaactgctt gaccaagcgg
     3661 cggaagcctg ccgggcggga atcccccgcg tacacctgct ggatgggaaa atgcgcggcg
     3721 tgctggtgga ggaacttttc tccgaagaag gcgtaggcac catggtccac acggactcct
     3781 accgggaaat acgcccgctg aaggaagaag acattccgga actcctctcc atgatcgccc
     3841 gttccgtggt ggactccaaa ctggtcaacc ggaattacga agacattgcc gccagaatgg
     3901 acagttacta cgtgctgacc ctggacgaca gcatcgtggg ctgcgttgcc gtttatccct
     3961 acccggagca ccacagcgcg gaactgggct gcctgtacat caagcaccgc catgaaggac
     4021 gcggctatgg ccgcgcacta tgtgaatttg ccaaaagaaa agcggaggaa atgggaatgg
     4081 aattcatctt cgccctctcc caaagtgccg tccattactt ccgggaccgc atgcattacg
     4141 cggaattttc ccgcgacagc ctgcctccgg aacgtctgcg caccctggaa ctcagcggcc
     4201 gcaaatccgg ggttttcggg ttaaggctga aatagttttc tattgaccaa tcttgccccg
     4261 ttcctgctta tgggcggaat tcactggtcc aaacgggcgg gcaactgttc ccggctgcgg
     4321 cttctccgga cctttttggt ttaactgctc tgatgatacg ccgcagcatc taaaacccat
     4381 tcccccctgt ctcctccgcc gcatggccaa aaacaatgat ttttttat
//
LOCUS       DAOMSU010000175         4417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1856_length_4417_cov_2.918615, whole genome shotgun sequence.
ACCESSION   DAOMSU010000175 DAOMSU010000000
VERSION     DAOMSU010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1856_length_4417_cov_2.918615"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..793
                     /locus_tag="PK077_07695"
     CDS             <1..793
                     /locus_tag="PK077_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HRN24220.1"
                     /translation="NFKDAPAARYIKLNVMEGVGDYGSGREIYVFKVPGTESYLPGDI
                     NNDGKIDRNDLTSYINYTGLRKGDSDFEGYISNGDINKNGLIDAYDISVVATQLEDEA
                     DQPQEEDAKKDEEEDKKEGADEKKKAAGEAKKKARVDGKILLSADKKRYARGDSVKVT
                     VKGRNLRLVNALSFALPYDPKDLEFVGVEVKNMKKMENLTNDRLHTNGTKALYPTFVN
                     IGDKEPVEGTSELFVLKFKARRDMKFQPKLTDGMVVDKKLNTKKL"
     gene            1044..>1577
                     /locus_tag="PK077_07700"
     CDS             1044..>1577
                     /locus_tag="PK077_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24221.1"
                     /translation="MLGAPDRPHDAMTGPGLISLFCFLMTAAYFLRGGMCAGAVLCMG
                     LAFFSFSRRGWLRRSVTFLLQASLLFWGAEAWRLARLWMMEGGPFLLWTSIPAAALLL
                     HAAAILWRRRGEKNLPVPELARSRVFSVSVLLLFLLDALVPFRLLMGERILPWQGVNG
                     LAILLLAWWGGYCAEGLL"
     assembly_gap    1578..1587
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1588..2376
                     /locus_tag="PK077_07705"
     CDS             <1588..2376
                     /locus_tag="PK077_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24222.1"
                     /translation="RRQVMWTVFASAFFLQFLLGVTVASSLLMTGKLHIPVPFMMISG
                     PVYREEGFFMLALFSVSVLMAGSSWCSHLCYFGVWDCLAAASSRRKGHPVPGGKKACD
                     WRWFSLAAAVGIPLLLSVWGVPLGYALAAAFAVAFTAPFAWKKSSENGVREYCSRFCP
                     MGLTASLLGRLSPWRMRVRETCTGCMRCASACRDLAISRGGGACRISRRCTLCRDCIS
                     RCPHGALSLGMAGPFSSVPSVRADMYFVTLVSVMHVVFLATARI"
     gene            2638..3123
                     /locus_tag="PK077_07710"
     CDS             2638..3123
                     /locus_tag="PK077_07710"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRN24223.1"
                     /translation="MTVQFIKVTKKDRVCYAPVPEPLEEGDKLEIMEMKDEALFPPAG
                     KNARISLSRNPMVFVKMNDGFQRILMKHILYVEAGGSYCTLHVHGMPAILVSAPLARV
                     SEHLDGEYFIRVHRSYLVNKLHIESIAGNFLQVGVVAIPISKFMKKKVLGSLNMLSFS
                     K"
     gene            3283..4122
                     /locus_tag="PK077_07715"
     CDS             3283..4122
                     /locus_tag="PK077_07715"
                     /inference="COORDINATES: protein motif:HMM:NF023085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HRN24224.1"
                     /translation="MAIDRSAAEAATSSLQSLPFGNIIGGPLVACVEAQAQAARTSWE
                     FIQNVGLYADGEEKKTVNVSFQFIKDGRMAQITVPLLTIVPIPYIAINSIDINFKANI
                     SASAASTETENSSSSVDTSVSASARCFWARGSMNASYSSKKDSSATRDSKYSVEYTMD
                     VAVHAGQDSMPAGMAKVLEMLNNSISVVSPEGFLDVQHAIQDDGTVKLITSYKNREGL
                     FDPEAITLRIGEATEIFENGAGNEARIVKTDSGKEYTLSREDARNCTVSAGELKRKVA
                     VGA"
     gene            4161..>4417
                     /locus_tag="PK077_07720"
     CDS             4161..>4417
                     /locus_tag="PK077_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24225.1"
                     /translation="MSRLNLIVESLLKDIIEAQHAANCHAASLARQYGKRGRACGFPL
                     PSAQIGSLEFDLKYAVTGTSGVEEKQVADTEQWSLFRQELE"
BASE COUNT          932 a         1125 c         1341 g         1009 t
ORIGIN      
        1 caacttcaag gatgctcccg cggcccgcta catcaagctg aatgtcatgg aaggcgtggg
       61 cgactatggc tccggcaggg aaatatacgt cttcaaggtg ccgggcacgg aaagctatct
      121 gccgggcgac atcaacaatg acggcaagat tgaccgaaac gacctgacct cctacatcaa
      181 ctacacgggg ctgcggaaag gcgattctga ttttgaaggc tacatcagca acggagacat
      241 caacaagaat ggtcttattg acgcttacga tatttccgtg gtagccaccc agctggaaga
      301 cgaagccgac cagccgcagg aagaagacgc caagaaagac gaggaagagg ataagaagga
      361 aggggccgac gagaagaaaa aagccgccgg ggaggcaaag aagaaagccc gcgtggacgg
      421 aaagattctg ctcagcgccg ataagaagag atacgccagg ggagactccg tcaaggtgac
      481 cgtgaagggc cggaacctcc gtctggtgaa tgccctgagc ttcgccctcc cgtacgatcc
      541 caaggatttg gaattcgtag gcgtggaggt taagaacatg aagaagatgg agaacctgac
      601 gaacgacagg cttcatacga acgggaccaa ggccctgtat cccaccttcg tcaacatcgg
      661 agacaaagag cctgtagagg ggacgtccga attgtttgtg ctgaagttca aggcccggcg
      721 cgatatgaag ttccagccga agctcacgga cggcatggtg gtggataaaa agctgaacac
      781 caaaaaattg taatttaaca tcctgatacg gctccatgcc cggccaaatg gaggccgcgc
      841 atggagcctg ccgttccggc ggagtatttc cgcagtattc atcgctgtgg agcggtgttc
      901 ttccgcgtgt tttggagaat ccggacggag cggcgacgcg gaaatatttt cctgcctcgc
      961 gtttccggcg gagcgtgacg caatggggct ctcttaacag gcggatgaac cgtttttttc
     1021 tggcgcgacc ggtcttttcc cttatgttgg gggcgccaga caggccccac gatgctatga
     1081 ccggccccgg actcatctcc ttgttctgct ttttaatgac ggccgcctat tttttgcgcg
     1141 gcgggatgtg tgcgggcgcc gttttgtgca tgggcctggc gtttttttca ttttcccgtc
     1201 gcggctggct tcgcaggagc gtcacgtttc tgctgcaggc ttccctgctt ttctgggggg
     1261 cggaagcctg gcgtctggcc aggctgtgga tgatggaagg ggggcctttc ctgctctgga
     1321 cttccattcc ggctgctgcg ctgttgcttc atgcggcggc cattctatgg cggaggcgcg
     1381 gagaaaagaa tttgcccgtt ccggagctgg cccgcagccg cgtattttcc gtttccgtat
     1441 tgcttctgtt tttgctggat gcgctggttc ctttccgttt gctgatgggg gagcgcatct
     1501 tgccgtggca gggggtcaat gggctggcga ttcttctcct ggcctggtgg ggcggctatt
     1561 gcgcggaagg tttgttgnnn nnnnnnnagg cggcaggtga tgtggaccgt gtttgcctcc
     1621 gctttttttc tccagtttct gctgggagtg acggtggctt cctccctgct gatgacgggg
     1681 aagctccata ttcccgtgcc gtttatgatg atttccggcc cggtttaccg ggaagaaggg
     1741 tttttcatgc tggcattgtt cagcgtatcc gtccttatgg ccgggagttc ctggtgcagc
     1801 catttgtgct acttcggggt gtgggactgc ctggccgccg cttcttcccg caggaagggg
     1861 catccggtgc cgggtgggaa aaaagcctgt gactggaggt ggttttccct tgccgcggcg
     1921 gtggggattc ccctcctttt gtccgtgtgg ggcgttccgc tggggtatgc cctggcggcg
     1981 gcgttcgccg tagctttcac ggctccattt gcctggaaga aaagttccga aaacggtgtg
     2041 cgggaatatt gcagcaggtt ctgccccatg gggctgaccg ccagtttgct gggcaggctg
     2101 tccccgtggc gcatgcgcgt cagggagaca tgcaccgggt gcatgcggtg cgcctccgcc
     2161 tgccgtgatc tcgccatctc gcgcgggggc ggggcctgcc gcatttcccg gcgctgcacc
     2221 ttgtgccgcg actgtatttc ccggtgtccg catggcgctt tgagcctcgg catggccggc
     2281 cccttttcct ctgtgccgtc cgtgcgggcg gatatgtatt ttgtaacgct ggtttccgtc
     2341 atgcatgtgg tcttcctggc cacggctcga atctgatttt atgttgccat gaaagatttc
     2401 tgctccctgg agtatccgtt ttgcggagga tggatggcat ctgtccatta ggaggctttt
     2461 tcagggctgc cgtcagaggg attcaggcag cggtgcagga gggggacggc cctgaattca
     2521 tggaacggca tggttccctc ttcccatccg aaagagtgga ttttggtttc agaaggtcat
     2581 ttttgttatt cattgagaga tttattgatg atttaaagtt tttgttatga tttttgaatg
     2641 accgttcaat ttattaaagt cactaaaaaa gatagggtct gctatgctcc ggtgccggag
     2701 ccgttggaag agggggataa gctggaaatc atggaaatga aggatgaagc ccttttcccg
     2761 ccggcaggga aaaacgcaag gatttcccta tccaggaatc ccatggtatt cgtgaaaatg
     2821 aatgacggat tccagcgcat cctgatgaag catatcctgt atgtggaggc gggcggaagt
     2881 tattgcacgc tgcacgtgca cggcatgccg gctatcctgg tatccgctcc gctggcgcgg
     2941 gtatccgagc atctggatgg ggaatatttt atccgcgtgc accgttccta cctggttaac
     3001 aagctgcata tagaatccat tgcgggcaac ttcctccagg tgggcgtcgt tgccattccc
     3061 atcagcaaat tcatgaagaa aaaagtgctg ggcagtttga acatgctgtc gttttccaaa
     3121 taagttccgg cctgccgcac gggctcctgc ggcgttccgg gaaagcgaaa tggaacggcc
     3181 ggaaaggcgg gatggaagca gaaacggaat ggccgcatca ttcacgacgt cgccggaagg
     3241 cggcatatca atgaatctaa accagaaata gaaattaaat atatggcaat tgacagatca
     3301 gcagcagaag cggcgacttc ctccctgcag tcgctgcctt tcggcaacat catcggcggt
     3361 cccctggtcg cctgtgtgga agcacaggcc caggcggccc ggacttcctg ggaattcatc
     3421 cagaacgtgg gcctgtacgc ggatggggaa gagaagaaga cggtgaacgt ttccttccag
     3481 ttcatcaagg acggccgcat ggctcagatt acggttcccc ttctgaccat cgtccccatt
     3541 ccctatatcg ccattaactc catcgacatt aatttcaagg cgaatatcag cgcgtccgcc
     3601 gccagtacgg agacggaaaa ttcctccagt tccgtagata ccagcgtttc cgcctctgca
     3661 aggtgctttt gggcgcgcgg cagcatgaat gccagctatt ccagcaaaaa ggactcctct
     3721 gcgaccaggg attccaaata cagcgtggaa tacaccatgg acgtagccgt ccatgccgga
     3781 caggacagta tgcctgccgg gatggccaag gtgctggaga tgctgaacaa tagcatttcc
     3841 gtcgtttctc cggaagggtt cctggacgtg cagcatgcca tccaggacga cggaaccgtg
     3901 aaattgataa cctcctacaa aaacagggag gggctgtttg acccggaagc catcaccctg
     3961 cggataggcg aagcgacgga aatatttgaa aacggtgcgg ggaatgaagc caggattgtg
     4021 aaaacggatt ccggcaagga atacacactt tccagggaag acgccagaaa ctgcacggtg
     4081 agcgctggag aactcaagag gaaggtggct gtaggcgcct gataaccgaa ccatagcgat
     4141 ttctcctcca ttccagagcc atgtcccgtc ttaatctcat cgtggaatcc ctgctgaagg
     4201 acattattga agcgcagcat gcggcaaact gccacgcggc atcactggcg cgccagtatg
     4261 ggaagagagg ccgtgcgtgc ggtttcccgc tgcccagcgc ccagataggc tccctggaat
     4321 tcgatttgaa atatgcggtg acgggaactt ccggggtgga ggagaaacag gttgcggaca
     4381 cagagcagtg gagcctgttc cggcaggaat tggaaga
//
LOCUS       DAOMSU010000176         4345 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1905_length_4345_cov_2.778322, whole genome shotgun sequence.
ACCESSION   DAOMSU010000176 DAOMSU010000000
VERSION     DAOMSU010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1905_length_4345_cov_2.778322"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="PK077_07725"
     CDS             <1..350
                     /locus_tag="PK077_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24226.1"
                     /translation="KDQDKDQDKDQDKDQDKDQDKDNAETKEGKRDENEMKNARLPSS
                     PEKDRLPETSGMEKPQPQPEDNKPVPAAAQKESREEKERREARAILMERRDIEPGCPV
                     PQRSPEIPPDKDY"
     gene            445..2820
                     /locus_tag="PK077_07730"
     CDS             445..2820
                     /locus_tag="PK077_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN24227.1"
                     /translation="MTVTLMAQQVVTTWLSDAVVPGEQTRLFIILTDGSIARQDPLPR
                     VKGASLRWVSQGNYIRWPGSPNQSAFLMAIEVTPDEVGTVEIPSLTLQANNGRTYTTL
                     PQTLTVYPYSAIKWNTLNLNGTAVPYGMLWHVDNQKPYVHQPDRCELKIYAPEYILEY
                     TAPAITTSNLATWRFEPTLVELLRGQPRGSVLYKGVNWNVMTFQSTLFPLRAGEVTAS
                     GTVTARAALPEADPLIATFIRSEVLVEMAIPEVKITARELPPGAPSSFTNAVGNFRIS
                     STTDARDLSANEPITVKIRVTGTGNIHSIACPALTDASNWKLYPPNKLDPGQNTRSIQ
                     GTVEFQQMMRPVAQTDAIPPFELTFFDPSTQQYKTVTTAPIPLEWKASAITGTAAGGA
                     APATPPPAGTIPVAEMTDIYGLMPATVMNALTAPAHWGWYFLAYIPAVILLGMALIRY
                     IRKIRKESFTARERMRAFRRLASTPRQASSTEFLRAAGNFIESYIPPSKQNEKMKDIL
                     KLRDDRAFKPSESSEELPHAERQHMLQAIKKAIAKLPVLMMAAVLALSLAGTTGNASE
                     TDNLGVKAYEQGEYAKAIDYFSKESGNMNLSKAERAYACFGIGNSYYRMNKPGLAALN
                     YRRALELSPYFTEAEYNLQFIERKEGAILPANTTENQWLTYIRHDTLGPISILSGAVM
                     LTFLALLTVTRRHGALLTILATLSGLVTVAAVMNYIMYPETPESIPADRLLVVTQKTP
                     GRHAADMNRPALITLPESTPLILRAERGSWYYASTFQNTPVWIPRTDAQPL"
     gene            complement(3174..4004)
                     /locus_tag="PK077_07735"
     CDS             complement(3174..4004)
                     /locus_tag="PK077_07735"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRN24228.1"
                     /translation="MMEHSVIFPDHRKVCALGQGTWKMGKSALREADEIDALRAGIEL
                     GMSVVDTAEMYGNEEMVGAAIRGLRDRVFLVTKVLPGNASRTGTKAACERSLNRLKTD
                     YVDLFLLHWGGPHPIEDTVASMIELQQEGKIKAWGVSNMDVPEMERFYAVPGGVSCAA
                     NQILYNLAHRGVEYDLLPWCRERHLPVMAYSPADEGRLSRNPVLMEIAQKHEATPVQI
                     ALAWILRCPGMIAIPKAGSVTHVQENYRSLSIRLTAEDVDLLDGAFPPPVRKVHLDSW
                     "
BASE COUNT         1066 a         1425 c         1012 g          842 t
ORIGIN      
        1 acaaggacca agacaaggac caagacaagg accaagacaa ggaccaagac aaggaccaag
       61 acaaggataa cgccgaaacc aaggaaggca agagggacga aaatgaaatg aaaaacgccc
      121 ggcttccctc ctctccggaa aaagacaggc tgccagaaac gtccggaatg gaaaaaccgc
      181 agccccagcc ggaagataac aagcccgttc cggcagccgc acaaaaggaa agcagggagg
      241 aaaaggaacg cagggaagca agagccatcc tgatggaacg cagggacatt gaacccggct
      301 gccccgttcc ccagcgttct ccggaaattc ctccggataa ggattactaa cccgttccgc
      361 ccgcatccgc gaagccaccc ctttaaacat atcccacaaa acaagatgaa aaaactgctc
      421 gccattcttt cgcttttctt ctgcatgact gtcacgctta tggctcagca ggtggttacc
      481 acctggctgt ccgatgccgt tgtgccggga gaacaaaccc gtctcttcat catcctgaca
      541 gacggaagca tcgcccgcca ggacccgctt ccccgggtaa agggcgccag cctgcgctgg
      601 gtcagccagg gcaactacat ccgctggccg gggtctccca accaatccgc cttcctgatg
      661 gccattgaag taacgccgga cgaagtgggc accgtggaaa tcccttccct gaccctgcag
      721 gccaacaatg gccgcactta taccacgctt ccccagacgc tgactgtcta cccttacagc
      781 gccatcaaat ggaatacgct gaacctgaac ggcacagcag tcccgtacgg catgctgtgg
      841 catgtggaca accagaaacc ctatgtccac cagccggacc gctgcgaact gaaaatttac
      901 gccccggaat acattctgga atacacggcg cccgccatca ccacctccaa cctggctacc
      961 tggcgctttg aacccacgct ggtggaactg ctccgcggcc agcctcgcgg ttctgtactt
     1021 tacaaaggcg tcaactggaa tgtcatgacc ttccagagca ccctgttccc gctgcgcgcc
     1081 ggggaggtaa cggcgtccgg cactgtcacg gcacgcgccg ccctgccgga ggcggaccct
     1141 ctcatcgcca ccttcatccg ttcggaagta ctggtggaaa tggccattcc ggaagtgaaa
     1201 atcacagccc gggaactccc cccgggagct ccctcttcct tcaccaatgc cgtagggaat
     1261 ttccgcatct cctccaccac ggatgcgcgg gatctcagtg ccaatgaacc cattactgtt
     1321 aaaatcaggg taacaggaac aggcaatatc cacagtatcg cctgcccggc cctcacagac
     1381 gcctccaact ggaaactcta tcctcccaac aagctggacc cgggccagaa tacgcgctcc
     1441 atccagggaa ccgtggaatt tcagcaaatg atgcgtccgg ttgcccaaac ggatgccatc
     1501 cctccctttg aactcacttt ttttgacccc agcacgcaac agtacaaaac cgtaaccaca
     1561 gcccccattc ccctggaatg gaaggcctca gccattactg gaacggcggc cggaggtgcc
     1621 gctcccgcca cgcctccccc ggcaggcacc attccggtag ctgaaatgac ggatatttac
     1681 gggctcatgc ccgccaccgt catgaacgcc cttaccgccc cggctcactg ggggtggtat
     1741 ttcctggcct acatccccgc cgtcatcctg ctgggaatgg ctcttatccg ctacatccgg
     1801 aaaatccgga aggaaagctt cacggccagg gaacgcatga gagccttccg caggctcgct
     1861 tccactccgc gccaggcaag ctccacggaa ttcctgcgtg ccgccggcaa ctttattgaa
     1921 tcctatattc caccttccaa gcagaatgaa aaaatgaaag atatccttaa actccgggac
     1981 gacagggcat tcaagccttc ggaatcttcg gaagaactgc cgcatgcgga acgccagcac
     2041 atgctccagg ctattaaaaa agccatcgcc aagcttcccg ttctcatgat ggccgccgta
     2101 ctggccctgt ccctggcagg aacaaccgga aacgcgtcgg aaacggacaa cctgggtgta
     2161 aaagcctatg aacagggaga gtacgccaaa gccattgact atttcagcaa ggaaagcggc
     2221 aatatgaacc tttccaaggc agaacgcgcc tacgcctgtt tcggcatcgg caattcctat
     2281 taccgcatga acaaacccgg cctggccgct ctcaattacc gccgtgctct ggaactctcc
     2341 ccgtacttca cggaagctga atacaacctg caattcatcg aacgcaagga gggcgccatc
     2401 ctgcccgcca acaccacgga aaaccagtgg ctcacttaca tccgccatga tacgctcggc
     2461 cccatctcca tcctgtccgg agccgtcatg ctcaccttcc tggccctgct gaccgttacc
     2521 cgcagacacg gggccctgct gaccatcctg gccaccctta gcggactggt cacagtagcg
     2581 gctgtcatga actacatcat gtacccggaa accccggaat ccattccggc ggatcggtta
     2641 ctggtcgtta cccaaaaaac gcccggcaga catgcggcgg acatgaacag acctgcgctc
     2701 atcacccttc cggaatccac cccgctcatc ctgcgcgcgg aacgcggctc ctggtactac
     2761 gccagcacct tccagaacac gcccgtctgg atacccagga cggacgctca gccgctgtag
     2821 cgctttatta tcccgcctcc tctggagccg cctctccgga atgccgtatt cccggaaggg
     2881 cggctttctt tcagccgcat tcaatcttct tccctactga agaagccgga accggaaaac
     2941 ggcacatcct ctgttcaaag ggaaaaaccg ggaagtcccc ttccggaaac gcgtcagaag
     3001 attccccggc cgcatgaaca aaaagccaat accctaccca caaggcgtgc gtgtattgaa
     3061 attacccgct ggcgcccttt catcccggac agatgaaagg cttgaaacaa ttctgatgcg
     3121 gcaggaaaat ccgcgcgtcc cgcttctccc caactgccgg acggcatcac ggtttaccag
     3181 gaatccagat gcacctttct gaccgggggc ggaaaagccc catccagcaa atccacatct
     3241 tccgcagtca gcctgatgga caaactccgg taattttcct gcacatgcgt gacggaaccg
     3301 gctttgggga tcgcaatcat tccagggcag cgcaaaatcc aggccagggc aatctgcacc
     3361 ggggtggcct catgcttctg cgcaatctcc atcagcaccg gattacggga aagccgccct
     3421 tcatccgcag gcgaataggc catcacggga aggtggcgtt cccggcacca tggcagcaaa
     3481 tcgtactcca cacctcgatg ggccagatta taaagaatct ggttggcggc gcaggaaact
     3541 cctcccggga cggcgtaaaa ccgctccatt tccggaacgt ccatattgct gacgccccat
     3601 gcttttattt ttccctcctg ctgcagctca atcattgaag caaccgtatc ttcaatggga
     3661 tggggacctc cccagtgaag cagaaacaaa tccacgtaat ccgtcttcag cctgttcagg
     3721 ctccgttcgc aggccgcttt agttcctgtc ctgctggcgt tgccgggcaa caccttggta
     3781 accaggaaaa cacggtcgcg caaaccgcgg attgccgctc ccaccatttc ctcgttgcca
     3841 tacatctccg ccgtatccac tacgctcatg cccagctcaa tgccggcgcg caaagcatct
     3901 atttcatcgg cttccctcaa cgctgacttt cccattttcc aggttccctg ccccagggcg
     3961 caaaccttcc gatggtccgg aaaaattact gaatgttcca tcattaatat cttctttcta
     4021 cctgatcgca tcattccatg ccgtacaaac ctttgacagc agaggagaaa aaaaagctca
     4081 aaaatcagga cgtttttcct gaaccgcctc acccgtcatt ttccctcttt tgctggcggg
     4141 acgtcaataa ctctcattga aatttcattc catatgcttc cagtgaacat ataagaaata
     4201 tccattccca gccgtgcata gcagcatcaa aaaaatgcac agacctccag tcataccgac
     4261 tccggaacaa cgggctctct tctggaaaaa tcacgcccgg tatcggtttt tcagccccct
     4321 tttttcactg ctgttttgaa aataa
//
LOCUS       DAOMSU010000177         4323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1920_length_4323_cov_2.706888, whole genome shotgun sequence.
ACCESSION   DAOMSU010000177 DAOMSU010000000
VERSION     DAOMSU010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4323
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1920_length_4323_cov_2.706888"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="PK077_07740"
     CDS             <1..1051
                     /locus_tag="PK077_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRN24229.1"
                     /translation="NALAEFKTGQVKHEALMGISYTGSSVYGDGSSLVTNATANTNGY
                     FSLYNPPPFFPAGRDYSGANATDTVVQRAGFLLQDVLSYGQWRFLAGVRGDAHFSLDN
                     NYAFAWSPRFGITRMFGERVALFANAARTSAPNFGYLDENGKELTDSWRTDQMEFGFR
                     VSPVDKVWFSASWFDIIQNNTPVAIDGYTNRYYSDGSKRAEGVELSLNGEITRNWSSY
                     LSYTYTRTKNRTTGEVYPTIAPNALALWQKYRIDGGLLNGTVLGLGYRCKDSYYATFR
                     GAKIADNYTIPSYSVFDFTVEIPLPESKWLKDATLRLAVYNIFDKKYVQSTRHAVQCT
                     VGEPRTFEVGLKTTF"
     gene            1067..1813
                     /locus_tag="PK077_07745"
     CDS             1067..1813
                     /locus_tag="PK077_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN24230.1"
                     /translation="MSSPVISVSHLKRSFKSGSGNITVLKDISLTVNRGEFVAVMGAS
                     GSGKSTLLNVLGGLLAPDSGTVTVDGMDLGSMSDASLTVYRRDRVGFIFQMFNLVGTL
                     NVEENILLPSLAGGRKVVPAALDAMIEKVGLSHRRHAMPDTLSGGEQQRVAIARALVS
                     GPALVLADEPTGNLDSENTRLMGDLFRDLHHAQGCAFVLVTHAPDVAMWADRVIVLKD
                     GSIVDDRSTAEFAGPAELSSFYERTLNDAL"
     gene            1810..>4323
                     /locus_tag="PK077_07750"
     CDS             1810..>4323
                     /locus_tag="PK077_07750"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN24231.1"
                     /translation="MRLLVKLIWRDLLASPGRMAVSVFAILVSVSLIVWMMGSYDTLV
                     REFDNDAEAYMGNYDLCLVPESPKGPLPPGQYPQFRDQELPARLAASPLVETVNAACQ
                     VPRLQIGCGNERGSFDEQMRDRMGIPPQSPILVGNHAVECPYELKEGVWPDMASSSAM
                     EGVLGSGSAKYFSAGVGTVMNVRVGTHVYDVKIVGIVKQAKATPGVIMGPGGMSGPAF
                     SSLFVPVKVCEKITGQPFAPNLIYVQLKEGVDKKEFAESFRQELVQAAAAVADTDSII
                     RRLSSDRSVRSQKDSAEMSVWLVLFSCIFIIFTTLSIGVSERARRLALMRALGLGHIQ
                     IALLIAGEGIFLCIPALLGGLGAGFFLVYLLEEGSASAPVMAWSTVLTAAVCAVGGAL
                     LASIIPAWRASRQSPLEAAVPSSGFIGKVSRVPVWSVAAGLACVCLQPAALLLPGLEA
                     ETRKWIFFWLGYPGLVAGALFLAPTFVRVTEWAGAWITGFLLRVPHSFLNMQLSRNLS
                     RSVGTAVSMSVGLSLFVGVQTWGYSMLVPFSPDTSTPGTLVSFLYTEFKSADVPELMA
                     RPSLRNSRMYPIYVDEPDIAPAQMKTPGFSGMRNRSIVLAGIPVEEMTGGSHPLFNPV
                     FVSGNPQEAYAMLESTRSLLIPDTFARAVGLKVGDDLMLVNPSSRERRPGNESPSGIR
                     GRGRGAAVRGEPWKVAGVVSFPGWHWLTKTSGMRVRRGGFVAALAIADERWLKEEYAH
                     QGFQFIWGDTAPGISNVELQNDLGEYALMKVRQQENGGEGARPLVKALTRESLGGSVT
                     SRGDDVIFTMSKLPIIIMVIAVLAVLNTVLASVQSRRREF"
BASE COUNT          793 a         1179 c         1341 g         1010 t
ORIGIN      
        1 caacgcactg gcggaattta agaccgggca ggtgaagcat gaagccctga tgggcatttc
       61 ttacacgggc agcagcgttt atggggacgg ttccagcctg gtgactaatg ctaccgcgaa
      121 cacgaacggc tatttttccc tgtacaatcc tcctcctttt ttcccggcag gacgcgatta
      181 ttccggcgcc aatgcgacgg ataccgtggt gcagagggcg ggttttctgc tgcaggatgt
      241 tctcagctat ggacagtggc gttttctggc cggcgtgcgc ggtgacgctc atttcagcct
      301 ggacaataat tatgcgtttg cgtggagccc ccgcttcgga attacccgca tgttcggcga
      361 acgcgtagcg ttgttcgcca acgcagcccg tacgtccgcc cccaacttcg ggtatctgga
      421 tgaaaacggg aaggaattga ccgattcctg gcgtacggac cagatggaat tcggtttccg
      481 ggtcagcccg gttgataagg tgtggttttc cgcttcctgg tttgacatca tccagaataa
      541 tacgcctgtc gccatagacg gatataccaa ccggtactat tcggacggct ccaagagggc
      601 ggaaggcgtg gaactttccc tgaacgggga aataaccagg aattggagtt cctatctttc
      661 ctacacctac acccgcacca agaaccggac caccggggaa gtgtatccca cgattgctcc
      721 gaatgctctg gccctctggc agaagtaccg cattgacgga ggattgctga acggtacggt
      781 gctgggcctg ggctaccgct gcaaggattc ctactacgct acgttccgag gcgcaaaaat
      841 tgcggacaac tacaccatcc cctcctatag cgtattcgac tttacggtgg aaatccccct
      901 gccggaatcc aaatggctga aagacgctac gctgaggctg gctgtgtata atatcttcga
      961 taaaaagtac gtgcagtcca cccgccatgc cgtgcagtgc acggtgggag agccacgtac
     1021 gtttgaagta ggcctgaaga ccacgtttta accttgcgtt tccgccatga gttcccccgt
     1081 catttccgtc agccatctca agcgcagttt caaatccggt tccgggaata tcactgtgct
     1141 gaaagatatc agccttaccg tgaaccgggg agagttcgtt gccgtgatgg gcgcaagcgg
     1201 ctccggaaaa agtactctgc tgaacgtgct gggagggctg ctggctccgg attccggcac
     1261 ggtgacggtg gatgggatgg atctcggttc catgtcggat gcctccctga cggtgtaccg
     1321 gcgggaccgt gtagggttca ttttccagat gtttaacctg gtgggcacct tgaacgtgga
     1381 ggaaaacatt ctgttgccgt ccctggcggg aggcaggaag gtggttcccg ccgccctgga
     1441 cgccatgatt gagaaggttg ggctgtctca ccgtcggcat gccatgccgg atacgctcag
     1501 cggcggggag cagcagcgcg tggcgattgc ccgtgcattg gtctccggtc ccgctctggt
     1561 gctggcggat gagcctacgg gcaacctgga ttctgaaaat acccgtctta tgggtgattt
     1621 gttccgggat ttgcatcatg cgcaaggttg tgcttttgtc ctggtgacgc atgcgccgga
     1681 cgtagccatg tgggcggatc gtgtaatcgt gctcaaggac ggcagcattg tggacgacag
     1741 gtccacagcg gaatttgccg gtcctgcaga attgtcctcc ttctatgaac gtacgctgaa
     1801 tgatgcctta tgaggttgct ggtcaaactg atttggagag atttgcttgc cagccccggt
     1861 cggatggctg tcagcgtgtt cgccatcctt gtctccgtca gcctcattgt atggatgatg
     1921 gggagctatg atacgctggt cagggagttt gacaatgatg cggaggccta tatggggaat
     1981 tatgacctct gcctggtgcc ggagtctccc aaagggcccc ttcctcccgg acagtatccg
     2041 cagttccgtg accaagagct gcccgcccgt ctggccgcct ctccccttgt ggaaacggtg
     2101 aacgccgcgt gccaggtgcc gcgcctccaa atcggctgcg gaaatgagcg gggcagcttt
     2161 gacgagcaga tgcgcgaccg catgggaatt cctccccaga gccccatcct ggtgggaaac
     2221 catgctgtgg agtgccctta tgaattgaag gagggagttt ggccggatat ggcttcctcc
     2281 tcagccatgg agggggtgct gggcagcgga agcgccaaat atttcagcgc gggagtggga
     2341 accgtcatga acgtgcgcgt gggaacgcat gtgtacgacg tcaagattgt gggcatcgtc
     2401 aagcaggcca aggccactcc cggcgtcatc atggggccgg gcggcatgtc cggcccggct
     2461 ttctcctccc tttttgtgcc cgtgaaggta tgcgagaaaa ttacgggaca gccttttgcc
     2521 cctaatttga tttacgttca gctcaaggaa ggagtggaca aaaaggaatt tgcggaaagt
     2581 ttccggcagg agctggtcca ggccgccgct gcggtggcgg atacggattc catcatccgg
     2641 cggctttcca gcgaccggtc cgtccgttca cagaaggata gcgcggaaat gtccgtctgg
     2701 cttgtcctgt tctcctgcat tttcatcatt tttacgacgt tgagcatcgg cgtcagcgaa
     2761 cgtgcccgca ggctggccct gatgcgtgct ctggggctgg ggcacatcca aattgctctg
     2821 ctgattgcag gagaggggat ttttctgtgc attccggcat tgctgggagg gctgggcgcc
     2881 ggttttttcc tggtgtacct gctggaggaa ggctctgctt ctgcccccgt gatggcctgg
     2941 tccaccgtat taaccgccgc cgtatgcgct gtgggcgggg ccttgctggc ttccatcatt
     3001 cctgcgtggc gcgcttcccg ccagtctccg ctggaagcgg ccgttccttc ctccggattc
     3061 atcgggaagg tgagccgtgt tcctgtgtgg tctgtcgcgg cgggtctcgc atgcgtatgt
     3121 cttcagcctg cggccctgtt gttgccgggt ctggaggcag aaacgcgcaa atggatattt
     3181 ttctggctgg gttaccccgg cctggtagcc ggcgcgctat ttctggcccc caccttcgtg
     3241 cgcgtgacgg agtgggccgg ggcatggatt accggatttc tgctgcgcgt tccgcattct
     3301 ttcctgaaca tgcagctcag ccgcaacctc agccgttccg tgggaactgc cgtatccatg
     3361 tctgtgggcc tgtctctttt tgtgggggtg cagacatggg gctattccat gctggttccc
     3421 ttttctcctg atacgtcgac gcccggaacc ctggtttctt tcctgtatac ggagttcaaa
     3481 tccgcggacg ttccggagct gatggcgcgc cccagtctgc ggaattcccg gatgtatccc
     3541 atctacgtag atgaaccgga tattgctccg gcacagatga agacccccgg tttttccggg
     3601 atgcgcaacc gttccatcgt gttggccggc attcccgtgg aggagatgac cggggggagc
     3661 catccgctgt tcaaccccgt gtttgtttcc ggaaatccgc aggaggctta tgccatgctg
     3721 gaatccaccc gttctctcct gattccggac acgttcgcgc gggcggtggg gttgaaggtc
     3781 ggggatgact tgatgctggt taatccgtct tcccgggaac gccgtcccgg aaatgagtct
     3841 ccttccggta ttcgcggccg gggcaggggg gccgccgtgc ggggagaacc ctggaaagtg
     3901 gcgggagtag tttccttccc cggctggcac tggttgacca aaaccagcgg aatgcgcgtc
     3961 cgccggggag gctttgtcgc tgctttggcg attgccgatg aacgctggct caaagaagaa
     4021 tacgctcatc agggattcca gttcatctgg ggggataccg ctccggggat tagcaacgtg
     4081 gaacttcaga acgacctggg ggaatatgcc ctgatgaagg tgaggcagca ggagaacgga
     4141 ggggaaggag ccaggcccct ggtgaaagcc ctgaccaggg aaagcctggg tgggagcgtt
     4201 accagccgcg gggatgatgt gatttttaca atgagcaagc ttcctatcat catcatggtg
     4261 atcgccgttc tggccgtgct caataccgtg ctggcttccg ttcagtcccg ccgccgggaa
     4321 ttc
//
LOCUS       DAOMSU010000178         4315 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1925_length_4315_cov_2.803521, whole genome shotgun sequence.
ACCESSION   DAOMSU010000178 DAOMSU010000000
VERSION     DAOMSU010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1925_length_4315_cov_2.803521"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..968)
                     /locus_tag="PK077_07755"
     CDS             complement(270..968)
                     /locus_tag="PK077_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRN24232.1"
                     /translation="MNITIHRASSRGHANHGWLDTYHTFSFANYFNPERMQFGALRVL
                     NDDKVLPEEGFGTHPHKNMEVVSIPLKGELRHGDSLNNSHTITRGEIQVMSAGTGIQH
                     SEFNGSSTEPLEFLQIWIIPDRVNAPPRYRDYNIAPLLKHNEISTFLAPETDISIMQQ
                     AWFSWAELNRGVEREYKFKCQNTGVYVFVIDGEIKIGDTVLHRRDGAGITDTDSITME
                     ALQNSTVLLMEVAV"
     gene            1174..>4315
                     /locus_tag="PK077_07760"
     CDS             1174..>4315
                     /locus_tag="PK077_07760"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HRN24233.1"
                     /translation="MRWIKGLIPTVIILLGALLLGCICYISIWGVPSFVVQRVEKALL
                     EKGIPSHIETLKVTLWPRAVVTLKNVELLDPEAMPEIRRPIVLLHEADVALNWKKLIE
                     GQVVPERVNVKGMNVTLPVDAGNLEKVFSVTGVNAELNLGRPGMVDIVKADAVVQGIR
                     VTAQGAFPIGQDDSGDFTLTAQDMAAVREQLNQILNYMDRVKWPDASPPRLIVNLSDD
                     PKGGVRIGMDLQAPFLRYGKIMVRDFLFNGDYADSVIMAKRFTMRDAETAGFVSLSLQ
                     ADLKKRTLIWDVRSTAPLVSWAVAIVDEALVPKEMKFLSEPHVQLSGRAVFSENWEGV
                     EHLNALGSGSMGAFSVLGEKFQRASCDFSYENGNFYVTDLDIRHPGGSFSGKVMGVDG
                     EIKIDVHSTLPMKAMLGMARSIAPEDAKLPPALEIRGDPELKVYGSVDMGKGWKGPFQ
                     VDRLQIEASVTDVFYQGVEFVSAAARAELIGRSVNVTQLDLVRDDGRVKLEGSYLGTD
                     LVFTLESNLKPELLETLAGNWFSVPEHLQLPEKAVLWVHGRLDIPEGKPVEPTLVRAR
                     IHAENLAWNKVPVKMANVEAEYRPNQLFVQNCRVEMEKGVFELFANGFLDGQMFVMGQ
                     STVPLQTIDQLLSMEDDDFFMNRFVFRKDSGLEFSFQGTLGLYNLEKAYDLQATISAT
                     NTRYRGVDLKSARADAHLVTDQLVLTNVTTVVSNGNYLSSAGLSGGPSECTLKAKGID
                     FRFVQDTVEVLGLEGQAYPGYTLRMFSDSAARVLKEFVFTRPVTLSGGGMFPMGDDMK
                     LMKGRIRFDASAGRVRYPLLGTTLDLGRTKGEVLISPQWVVVDKMMGTIWDGTFTGRV
                     LAQIDDGDALNGSFVLQDMNLTSIGKSYDKKMEKATVHGAIEFSSKGGNMNSIQAKGE
                     AALVHGDLVEIPLFGFLGEALSNYIPGLGHLINYKISRADCDFSIEKGYVRTSNFVAQ
                     GSNMSLEGGGWIRLSDLQVNSDFKLGLRGLPGFITSPVFLLAGGLFQVRGTGPLSNVS
                     WNFAPFSGGKAPVPPAA"
BASE COUNT          923 a          982 c         1326 g         1084 t
ORIGIN      
        1 ttgttccctg gaaaaaaggg ggaagtaatt aagtacatcc gcatggcagg ctatcaggaa
       61 ggggaaatta atgatcttgt ggaccgtacg gtgggacggg attccagcac ggaattcctg
      121 tacaagggac gcagtaaaaa ggatgccaga taaaaccttg ttaattttcc tttttgatga
      181 tcctaagcca ttcgtgattg tagctggaag ctgaaaatac gggagatccc agtggttttc
      241 ttcattaagc cggaacattt ttccgatcgt cagacggcca cttccatcag cagaacggtc
      301 gaattctgga gggcctccat ggtgatggag tccgtatccg tgatgccggc tccgtcccgg
      361 cggtgcagca ccgtgtctcc gatcttgatt tctccgtcta tcacgaagac gtacaccccc
      421 gtgttctggc acttgaattt gtattcgcgc tccacgccgc ggttcagctc cgcccaggag
      481 aaccaggcct gctgcatgat ggagatgtcc gtttccggag ccaggaaggt ggaaatctca
      541 ttgtgtttca gcaggggagc aatgttgtaa tcccggtacc tgggaggagc gttgactctg
      601 tccggaataa tccatatttg caggaattcc aggggctccg tggagctgcc gttaaattcg
      661 ctgtgctgga tgcccgttcc ggcgctcatc acctggattt ctccgcgcgt gatggtatgg
      721 ctgttgttca ggctgtcccc gtggcggagt tctcctttca gggggatgga aaccacttcc
      781 atatttttgt gcggatgggt gccgaatcct tcttccggga gaaccttgtc gtcgttgagc
      841 acgcgcagag ctccgaattg catgcgttcc gggttgaaat aattggcgaa gctgaatgtg
      901 tggtatgtat ccagccatcc gtggttggca tgtccacggg aggatgcacg gtgaatggta
      961 atgttcataa ttgatagggg gagtgggagc ggttttccgc ctgcagaatg tttgaccggc
     1021 tgcaactcgg ggttgttcaa ggcgagtgac attatttcca gaaccgtaaa acaaatcggc
     1081 aacacaaatt ggtggtgcaa tgtctcttct tttagggtag cgtgttgcag tacccgtgca
     1141 aagaagacaa ggcttatggc tcagcggaca tttatgagat ggatcaaggg actgattccc
     1201 acggtgatta ttttgctggg ggcgttgctg ctgggctgca tatgctacat ctccatctgg
     1261 ggggtgccat ccttcgttgt gcagcgggtg gagaaggctc ttctggaaaa gggcattccc
     1321 tcccatatag agacgctgaa agttactttg tggccccgtg ccgtcgtgac actgaaaaat
     1381 gtggaattgc tggatccgga ggccatgccg gaaattcgcc ggcccatagt tctgctgcat
     1441 gaggcggatg tggcgctgaa ttggaaaaaa ctgatagaag gacaagtagt gccggagcgg
     1501 gtgaatgtga aggggatgaa cgtcactctg cccgtagatg cggggaatct ggaaaaagtt
     1561 ttttccgtaa cgggggtgaa cgcggaattg aatctggggc ggcccggcat ggtggacatc
     1621 gtcaaggcag atgccgttgt gcagggcatc cgggtgacgg cccagggggc atttcccatt
     1681 ggtcaggatg attccggaga ctttacgctg acggctcagg acatggccgc cgtcagagag
     1741 cagttgaatc agatcctgaa ttacatggac cgggtgaagt ggcctgatgc atctcctccc
     1801 cgcctgattg tcaatttaag cgacgatccg aaaggcggcg tgcgcatagg catggatttg
     1861 caggcgcctt ttctcaggta cggaaaaatc atggtcaggg atttcctgtt caatggggat
     1921 tacgcagatt ccgttatcat ggccaaacgc tttaccatgc gggatgcgga aacggccggt
     1981 ttcgtcagcc tttcccttca ggccgatttg aaaaagcgca cgctgatctg ggatgtccgc
     2041 agtacggctc cgctggtctc ctgggcggtg gctattgttg atgaagctct ggtgccgaag
     2101 gaaatgaagt ttctgagcga gccgcatgta cagctatccg gtcgtgcggt tttttcggaa
     2161 aattgggagg gcgtggaaca tttgaatgct ttaggttccg ggtccatggg tgcattttcc
     2221 gtgcttgggg aaaaattcca gagggcctcc tgcgatttca gttatgaaaa cggtaatttt
     2281 tatgtaactg atctggatat aaggcatccc ggaggcagct tttccggcaa ggtaatggga
     2341 gtggacggag agattaagat agacgttcac agcacgctgc caatgaaggc gatgttgggg
     2401 atggcccgtt ccattgcccc ggaggatgcg aagctgccgc cagcgctgga aatcagggga
     2461 gacccggaat tgaaggtgta cggttccgtg gatatgggaa agggttggaa agggccgttc
     2521 caggtggacc gcctccagat tgaggcttcc gtgacggatg tcttttacca gggagtggaa
     2581 ttcgtttccg ctgcggccag ggcggaattg attggccgtt ccgttaatgt cacccaattg
     2641 gatttggtgc gggatgacgg ccgggtgaag ctggaaggaa gctatctggg caccgacctt
     2701 gtttttacgc tggaatctaa tctgaaacct gaactgctgg agactctggc tggaaattgg
     2761 ttttctgtcc ctgaacattt gcagcttccg gaaaaagcgg ttttatgggt acacggcagg
     2821 ctggatattc cggagggcaa gcctgtggag ccgactctgg tccgagcacg catccatgcg
     2881 gagaatcttg cctggaacaa ggttcctgtc aaaatggcga atgtggaagc ggaataccgt
     2941 cccaaccagc tttttgtgca aaactgccgg gtggaaatgg aaaagggtgt ctttgaattg
     3001 ttcgccaacg gttttctgga cggtcagatg tttgtgatgg ggcagtccac agttccgctg
     3061 caaacgatag accaattgtt gagcatggag gatgacgact ttttcatgaa ccgctttgtc
     3121 ttccgcaagg attccggcct agagttttcc ttccagggaa cgttgggttt gtacaatctg
     3181 gaaaaggctt acgacttgca ggctactatt tctgcaacca acacgcgcta caggggggtg
     3241 gacttgaagt ccgcccgggc ggatgcccat ctggtgacag accaactggt gctgaccaat
     3301 gtgacgactg tggtttccaa cggaaattac ctgtcttccg ccggattgtc cggaggtcct
     3361 tcggaatgca cgctcaaggc caagggcata gatttccgtt ttgtgcagga tacggtggaa
     3421 gtgcttggcc tggaaggaca ggcctatccc ggctataccc tgcggatgtt ttctgatagt
     3481 gccgccaggg ttttgaagga gtttgtcttt acccggcccg taacgctttc cggcggtggc
     3541 atgtttccga tgggggatga catgaagctg atgaaggggc gcatccgctt tgacgcttcc
     3601 gccgggcgcg tgcgctatcc cctgctggga accacgctgg atctcggaag gacaaagggg
     3661 gaagtgttga tttccccgca atgggtggtt gtggacaaga tgatgggaac catctgggac
     3721 ggaaccttca cgggccgcgt tctggcgcag attgacgatg gggatgccct gaacggttct
     3781 tttgtcctcc aggacatgaa tttgacgtct atcgggaaat cctatgacaa aaaaatggag
     3841 aaagccactg tgcacggagc cattgagttt tcatccaagg gcggcaatat gaattctatt
     3901 caggcgaagg gggaggctgc cctggtgcat ggcgacctgg tggaaattcc cctgttcgga
     3961 tttttggggg aagccctctc taattacatt cctggactgg ggcatctgat taattacaag
     4021 atatccaggg ccgattgcga tttttccatt gagaagggtt atgtgcgcac cagtaatttc
     4081 gtggcccagg gaagcaacat gtctctggag gggggaggct ggatacgcct ttccgatttg
     4141 caggtcaatt cagatttcaa gctggggctt cgcggtttgc cggggtttat tacttccccg
     4201 gttttcctgt tggccggagg gctgtttcaa gtgcggggaa cggggccttt gagcaatgtt
     4261 tcctggaatt tcgctccgtt ttccggaggg aaagcccccg ttcctcccgc cgcgg
//
LOCUS       DAOMSU010000179         4293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1933_length_4293_cov_2.667532, whole genome shotgun sequence.
ACCESSION   DAOMSU010000179 DAOMSU010000000
VERSION     DAOMSU010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4293
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1933_length_4293_cov_2.667532"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PK077_07765"
     CDS             complement(<1..1284)
                     /locus_tag="PK077_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRN24234.1"
                     /translation="MENNRIKPVDVASELSTSFLDYSMSVIISRALPDVRDGLKPSQR
                     RILYAMKELNLSPGGKTRKCSKIVGDTMGNYHPHGDAAIYGTLVNMAQDWSMRDILVI
                     GQGNFGTPDGDPPAAARYTEAKLSGMGVALMADLDKDTVDFVPTYDNEHTEPTVFPSA
                     FPNLLVNGGTGIAVGMATNMPPHNLGEVVDGICAVIDNPHMTVEELRQYIKGPDFPTG
                     GDVLGFSGIDNYFRTGRGSVRIRGKIDMEVTDSGKDLLIIREVPYGVNRAALQERIAE
                     LYKDKILTDISGIRDLSDEKTCIEIELKRDARPQVVMNQLYKLTSMETSFAVNMLAIH
                     DNRPKTLAMMDAINFYIEHRREVVVRRTRYLLGDAEKDAERLEAFLLALHHMDDFISI
                     IRDSKNRDEARERLQNYTFSTQTAESLGILIRSQAS"
     gene            complement(1308..3866)
                     /gene="gyrB"
                     /locus_tag="PK077_07770"
     CDS             complement(1308..3866)
                     /gene="gyrB"
                     /locus_tag="PK077_07770"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRN24235.1"
                     /translation="MSEDTNEVVETTVSTTAQHEYGAAQIDKLEGLEAVRKRPGMYIG
                     DPDERGLHHCVFEVLDNSIDEHLAGYCSKIDIVIHVDGSISIVDNGRGIPVDIHPKFG
                     IPAVELVLTNLHAGGKFGQGAYKYSGGLHGVGAKCVNALSDWFKAEVRRDGKRYQIKF
                     ERGKTVEPLRTVGSCACEVTGTTITFFPDATIFTDTTEFKFDRLTTRLRELAFLNPGL
                     VIELIDEREAPARRETFFYKDGIVEFVRQLGTNKEVINEDPISISGVRKVEVEYDGKK
                     MEDDVLVDVVFQYNNTYETNILCFANSIYNGDGGTHLSGFRGALTRVINGYAKANGLL
                     KEKDPSLSGEDVREGLVAVISVKMPNPRFSSQTKDKLVNTEIEGVVGNVVYEEIKTYF
                     EENPAMAKKIIEKSITAARAREAARKARETVRKSALSIGGLPGKLADCSDRDPAKCEL
                     FIVEGDSAGGSAKMGRDRRTQAILPLRGKVLNVEKARLDKALGSKEIQNMITAIGAGI
                     GEGDDEASFKLDRVRYHKIIIMTDADVDGAHIRTLLLTFFCRHMPQLVRAGYLYIAQA
                     PLYRIIRRKKEEYVQDDVALNHKLIELAVNDVTLRFADGSRSFSPEELSAILETLVNL
                     QRYTESMQAQGGSLEDLLSHREANGEFPEFLVKVRCGNEEEILFFHDMEALTAFSDEN
                     RDLFIFGMPSEEELLENPLPEREGPSRRSITHELHEAKAITRALARLAELGIPGNMIV
                     SMDTPLFELVEGEGDKEKVTPLFSVMDILESVISIGKRGVEITRFKGLGEMDAKDLFK
                     TTMDPERRELLRVILNDDNAVRADEMFTILMGDVVEPRKNYIVDHALNVRNLDI"
     gene            complement(4052..>4293)
                     /locus_tag="PK077_07775"
     CDS             complement(4052..>4293)
                     /locus_tag="PK077_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24236.1"
                     /translation="ENPRRRHPTHPRKAFLLTLQSRSGTTPERLCCLRKASGVARMGQ
                     KQNSGKKKEYAVPTETMALFPSSPPVKRLILSSNL"
BASE COUNT          823 a         1217 c         1265 g          988 t
ORIGIN      
        1 ggaggcctgg gaacggatca ggatgcccag gctttccgcc gtctgcgtgg agaatgtgta
       61 attctgcagg cgttcacggg cttcatcccg gtttttggaa tcccggataa tggaaatgaa
      121 gtcatccatg tggtgcaggg ccagcaggaa ggcttccagg cgctcggcat ccttctccgc
      181 atcccccagc agatagcgcg tgcggcgcac gaccacctcg cggcggtgct cgatatagaa
      241 gttgatggcg tccatcatgg cgagcgtctt ggggcggttg tcgtgaatcg ccagcatatt
      301 caccgcaaag gaagtttcca tggaggtgag cttgtaaagc tggttcatga ccacctgcgg
      361 gcgggcatcc cgcttcagct cgatttcaat gcaggttttc tcatcggaaa ggtcacggat
      421 gccggaaata tccgtcagga ttttatcctt gtacagttcc gcaatgcgtt cctgaagggc
      481 ggcgcggttc acgccatacg gcacttcccg gatgatgaga agatccttgc cggaatccgt
      541 tacttccata tctatcttgc cgcggatgcg cacggagccg cgcccggtgc ggaagtagtt
      601 gtcaatgcct gaaaagccca gcacgtcgcc cccggtggga aaatccggcc ccttgatgta
      661 ctggcgcagc tcttccacag tcatgtgggg attgtcaatc acggcgcaaa tgccgtccac
      721 cacttccccc aggttgtgcg gaggcatgtt ggtggccatc cccacggcaa tccccgtgcc
      781 gccgttcacc agaaggttcg ggaaagcgga ggggaacacg gtgggttccg tgtgttcatt
      841 gtcgtatgtg ggaacaaaat ccacggtgtc cttgtccaaa tccgccatca gggccacgcc
      901 catgccggag agcttggctt ccgtgtatcg ggcagcggcg ggggggtctc cgtccggcgt
      961 gccgaagttg ccctggccga tcacgagaat atcccgcatg gaccagtcct gcgccatgtt
     1021 caccagggtg ccgtaaatgg cggcatcccc gtgagggtgg tagttaccca tcgtgtcgcc
     1081 cacgattttg gagcatttgc gggtcttgcc cccgggggac agattgagtt ccttcatggc
     1141 gtacaggatg cgccgctggg aaggtttgag gccgtctctc acgtccggca gggcgcggga
     1201 gatgatgacg gacatggagt agtccaggaa agaagtggag agctcactgg ctacatccac
     1261 cggtttgatt ctattatttt ccatggtgct gaaagagagt gcgggggtta aatgtcaagg
     1321 ttgcggacat tgagggcatg gtccacgatg taattcttgc ggggctccac cacatccccc
     1381 atcaggatgg tgaacatttc atcggccctg acggcgttgt catcgttcag gatgacgcgg
     1441 agcagctcgc gccgttccgg gtccatcgtc gtcttgaaca agtccttggc atccatttca
     1501 cccaaccctt tgaatcgcgt gatttccacc ccgcgcttgc cgatggagat gacggattcc
     1561 agaatgtcca tgacggaaaa caggggggtt actttttcct tatccccttc accctccacc
     1621 agttcaaaca ggggcgtatc catggagaca atcatattcc ccggaatgcc cagttccgcc
     1681 agccgtgcca gagcccgcgt gatggctttg gcttcatgca gttcatgggt gatggagcgg
     1741 cggctggggc cctcccgttc cggaagcgga ttttccagca gctcctcttc cgagggcatg
     1801 ccgaagatga aaaggtccct gttttcgtcg gaaaaggcag tcagggcctc catgtcatgg
     1861 aagaagagta tttcctcttc attgccgcag cgcaccttca ccaggaattc cgggaattcc
     1921 ccgttggcct cccgatggct gagcaggtct tccagggaac cgccctgcgc ctgcatggat
     1981 tccgtatagc gctggaggtt gaccagcgtt tccagaatgg cggagagttc ttcgggagag
     2041 aaggaacggg agccgtctgc gaaacgcagg gtcacgtcat taacggccag ttcaatcagt
     2101 ttgtggttca gggccacgtc atcctgcacg tactcttcct ttttcctgcg gatgatgcgg
     2161 tacaggggcg cctgagcgat gtacagatac ccggcgcgca ccagctgcgg catgtggcgg
     2221 cagaagaaag tgagaaggag ggtgcggatg tgggcgccgt ccacgtcggc atcagtcatg
     2281 atgatgattt tgtggtagcg cacgcggtcc agcttgaagg aggcttcatc gtccccttcc
     2341 ccgattccgg cgccgatggc cgtaatcatg ttctggattt ccttgctccc cagggctttg
     2401 tccaggcggg ccttttccac gttcaacact ttcccgcgca ggggcaggat agcctgcgtg
     2461 cgtcggtcgc gccccatctt ggcggaaccg cctgcggagt caccttccac aatgaacagc
     2521 tcgcacttcg cgggatcccg gtcggaacaa tccgccagtt tgccggggag gccgccgatg
     2581 gagagggcgc ttttgcggac tgtttcgcgc gccttgcggg cggcttcccg ggcacgggcg
     2641 gccgtgatgc tcttctcaat gatcttcttg gccatcgccg ggttttcttc aaagtacgtc
     2701 ttgatttcct cgtacacgac attgcccacc acgccttcaa tctccgtatt caccagcttg
     2761 tccttcgtct gggaggagaa acgcgggttg ggcatcttga cggaaatgac cgccaccagg
     2821 ccttcacgaa cgtcttcgcc gctcagggag ggatcttttt ccttcagcag gccgttggcc
     2881 ttcgcgtaac cgttgatgac acgcgtcagg gcgccgcgga aaccggaaag gtgcgttccc
     2941 ccgtccccgt tgtaaatgga gttggcaaag caaaggatgt tcgtttcata cgtgttgttg
     3001 tactggaaca caacgtccac cagaacatcg tcctccatct tcttgccgtc gtactccact
     3061 tccaccttcc ggacgccgga aatggaaatg gggtcctcgt tgatcacttc cttgttcgtg
     3121 cccagctgac gtacgaactc cacaataccg tccttataga agaacgtttc ccggcgcgcc
     3181 ggagcttcac gctcgtcaat cagttcaatg accaggccgg gattcaggaa agccagttcc
     3241 cgcagacgcg tcgtcaggcg gtcaaacttg aactccgtgg tatccgtgaa aatggtggcg
     3301 tccgggaaaa acgtgatcgt ggttcccgtg acttcacagg cacaggaacc tacagtgcgg
     3361 aggggttcca ccgtcttgcc tcgctcaaac ttgatctggt aacgcttgcc gtcccggcgc
     3421 acctccgcct tgaaccagtc ggacagggcg ttcacgcatt tggcgcccac accgtgcagg
     3481 ccgccggagt acttgtacgc gccctggccg aatttgccgc ccgcgtgcag gttggtcaga
     3541 accagctcca cggcgggaat gccgaacttc gggtggatat ccaccggaat gccgcgcccg
     3601 ttatctacga tggaaatgga accgtccaca tgaatgacaa tgtcaatctt gctgcaatag
     3661 ccggccagat gctcgtcaat ggagttgtcc agcacttcaa atacgcaatg gtgcaagcct
     3721 cgttcgtcag ggtccccgat gtacatgccg ggccgcttcc gcacggcctc caggccttcc
     3781 agtttgtcaa tctgcgcggc gccgtattca tgctgcgccg tcgtggaaac ggtggtttca
     3841 acaacctcgt tggtatcttc ggacataacc gaaatatact acgcttatct taattttttg
     3901 caaggctaat attataaaac gcgtgcgcgc gcgtagggcc tagccttgca aaattccgct
     3961 gaaatcgcat aaaaaaggcc ctattgctcg cgttccgttc ttttccggct ccggacagcg
     4021 gagagggctt tcggaaacat ttggcgcgtt tttacagatt tgaggagagg atgaggcgtt
     4081 tcacgggagg ggacgaggga aaaagggcca tggtttccgt ggggacggcg tattcttttt
     4141 tcttgccgct gttttgtttt tggcccatcc gggcgacccc agaggctttc ctcaggcaac
     4201 agagccgttc cggagttgtt ccggaacggc tctggagggt caggaggaag gcttttcggg
     4261 ggtgggtggg gtgccttctc cttgggtttt ctt
//
LOCUS       DAOMSU010000180         4290 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1941_length_4290_cov_3.233294, whole genome shotgun sequence.
ACCESSION   DAOMSU010000180 DAOMSU010000000
VERSION     DAOMSU010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1941_length_4290_cov_3.233294"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK077_07780"
     CDS             <1..514
                     /locus_tag="PK077_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24237.1"
                     /translation="CQIFKTFFSPEYDGPFPGLQGFCLWIAFGVVMRIPVEVKFGDAF
                     FRRKDVCDKAGSQCHAEFNGIEILIAGIPDLVKNGKSTFAGWTRLGEHVEYFRVAGKP
                     FPEDLILEFLLSERFPSFGSIEKLVQAAQCRHLRGLNQFSYFVGGKRAGIKPAAQDFP
                     DFTGNEGGPP"
     gene            complement(945..2558)
                     /locus_tag="PK077_07785"
     CDS             complement(945..2558)
                     /locus_tag="PK077_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine exchanger family transporter"
                     /protein_id="HRN24238.1"
                     /translation="MFDILLQHNTLVLFGIIFFGIALGSIKIYGLNLGLSGVLFVALA
                     AGHYRLAIPDGVGQLGLALFVYCVGLSAGNRFFSAIAKQGSKLAIMSAVIVGVSALFT
                     CLFGIWFKVPAGISSGIFAGACTSTPALAAATESLPGASGSGVSIGYGIAYPFGVVGV
                     VLFVQLLPRLLRFDLNKEAKAMHSRQEVSIISRLVRVTQPNLIGQNIADCKLLDGLHC
                     MVTRVVRNDRLMPLTPEDTFREGTEVLIVGNSDTMPHDIAFLGEVCDNPYPMDSQKER
                     RKLILTNKNYAGHKLRDLKTLRTDGVIISRISRLGFTFVPTGETTLERNDVLTVVGSP
                     ENLTKYGDKIGHRSQDMNQTDLLSLGFGLSLGIILGLVNFSLGNGAGISLGIAGGPLI
                     VALLLGHFGRLGPIVGYIPRPTRLLLQDLGLVFFLADAGIKGGADLVDTVATHGAAVF
                     IMGVIVTCSGMFAGYFVGMKVFKMNILECLGGICGGMTSTPALGAIASKTDSQAPVVS
                     YATAYPVALILMTIVAKVIIQTIGGVVGI"
     gene            complement(2679..3158)
                     /locus_tag="PK077_07790"
     CDS             complement(2679..3158)
                     /locus_tag="PK077_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRN24239.1"
                     /translation="MEKINIICVDDQPEVLDSVLRDLRPLNSCFRLEGVESASECREL
                     LEEFDQDGELTGLVISDHVMPGGSGVELLGGIAKDDRFAGTRKILLTGQATHADTIQA
                     VNDAHIDNYLEKPWDPAVLLTTARKLLTRFIMEKGIDHTPYMSCLDQATLLDYLRRN"
     gene            complement(3145..>4290)
                     /locus_tag="PK077_07795"
     CDS             complement(3145..>4290)
                     /locus_tag="PK077_07795"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRN24240.1"
                     /translation="DLIAETDLELAYIDTATPAVDVEHYGTLLEQFVPAIVHELAARN
                     TRVFDRTAEKEEALRMLHRSEMAATLGQLSAGIAHELNNSLGVLSRKTDFVLETLDNM
                     FRQENAFRYTLYNKGLEGASSASTSEQRRLARQFEKNYGLSQDVAKALALIPDIQNQP
                     ELLDKPFLSQLEQNLPYWELGHDLHDMKLAVRHATAIVRSVRLLGGGNTRREEGVNVA
                     DSLTEAVALVKSSLRGVDFEMVLPPRDSVPGIFADMTELVQIWINLLQNACDALKIEN
                     TPNPAIHVECRVEKISSKDQTSHTTREILVSVTDNGPGVPKDLHEKIFRPNFTTKKKG
                     LSFGLGLGLTIVRRIVDSYGGRIRLESVPGHTVFTIIIPTINNHGKN"
BASE COUNT          945 a         1077 c         1308 g          960 t
ORIGIN      
        1 atgccagatc ttcaagacct tcttttcccc ggaatacgat ggcccattcc cagggttgca
       61 aggtttctgc ctgtggatag catttggcgt ggtgatgaga atcccggtag aagttaaatt
      121 tggcgatgca ttcttcagga gaaaagatgt ttgcgacaag gccggctccc aatgccatgc
      181 tgagttcaac gggattgaaa ttctgatcgc tggtattcca gatttggtaa aaaacggaaa
      241 gagtactttt gccggctgga ccagattggg cgagcatgtt gaatacttcc gggtggctgg
      301 aaagccattt cctgaagacc tgatccttga gttcctcctg agtgaaaggt ttccctcctt
      361 cggcagtatt gaaaaactgg tgcaggcagc acaatgccgc cacttgaggg gattgaatca
      421 gttttcgtat tttgtcggag gtaagcgggc cgggatcaag ccggctgcgc aggacttccc
      481 ggattttacc ggcaatgaag gaggaccgcc ctgaagaaag cagtgtttgt atgtctgctt
      541 cagatgaagc cagcgtgcca aagctctcta tagacatgag catgctgaag aggaaaagcc
      601 agaggatgtt tttcataagg tatgaaagac agacttaaat gcatggcaac ataaattgaa
      661 aaaatgtcaa taaaatatga tttttttgaa tataaaaaat acatttcata tttcgctttt
      721 atatcaaaat gatattgatg gcaaaaggat tttttcaaat ggaaaattgg ggtcattggc
      781 tctgagagac attggatttt cggagccgaa tccgggatga tttttgtgtc tatccctccc
      841 gcaagatgat gaaaacgggg ttccattcgg aaaaagaatg gaaccccgtg ctgaagcggt
      901 aaaacagaga taatagtttt caggtcagat gatctctgcc attgtcaaat tcccaccacg
      961 ccgccgatgg tctggatgat gaccttggcg acgatggtca tcaggataag ggctacggga
     1021 taggcggtgg cgtagctgac cacgggggcc tgggagtccg tcttgctggc gatggctccc
     1081 agggccgggg tggaggtcat gccgccgcag atgccgccca ggcattccag aatgttcatc
     1141 ttgaagactt tcatgcccac gaagtaaccg gcgaacatgc cggagcaggt gacgatgacg
     1201 cccatgatga agacggccgc gccgtgggtg gctaccgtat ccaccaggtc cgctccgccc
     1261 ttgatgccgg cgtcagccag gaagaagacc agccccagat cctgcaaaag aaggcgggtg
     1321 gggcgtggga tatagccgac gatggggccc agacgtccga agtggccgag cagcagggcg
     1381 acgataaggg ggcctcccgc aatgcccaga gagatgcccg cgccgttgcc gaggctgaag
     1441 ttgaccaggc ccaaaatgat gcccagggag aggccgaagc ccagggagag caggtccgtc
     1501 tggttcatgt cctggctgcg gtggccgatt ttgtccccgt atttggtaag gttttccggg
     1561 gagcccacca cggtcagcac atcgttgcgt tccagcgtgg tttcccccgt ggggacgaag
     1621 gtgaagccca ggcgggaaat gcgggagata atgacgccgt ccgtgcgcag ggttttcagg
     1681 tcgcggagct tgtgcccggc atagttcttg tttgtcagaa tcagtttgcg gcgctccttc
     1741 tgggagtcca tggggtaggg attgtcgcat acctccccca gaaaggcgat gtcgtgaggc
     1801 atggtgtcgc tgttgcccac aatgaggact tccgttcctt cccggaacgt gtcttccggc
     1861 gtcaggggca tcagcctgtc gttgcgaacc acgcgggtga ccatgcagtg cagaccgtcc
     1921 agcagcttgc agtccgcaat gttctggcct atcaggttgg gctgtgtgac gcggacaagg
     1981 cgggaaatga tgctgacttc ctgcctggaa tgcatggctt tggcctcctt gttgaggtca
     2041 aagcgcagca ggcgcggcag gagctggacg aaaaggacca cgcccaccac gccgaacgga
     2101 taggcgatgc cgtaaccgat gctgacgccg cttccggagg ctcccggcag gctttccgtg
     2161 gcggccgcca gcgccggggt gctggtgcat gcgccggcaa agatgccgga agagattccc
     2221 gcgggaactt tgaaccagat gccgaatagg caggtgaata acgctgacac tcctacaatc
     2281 accgcagaca tgatggccag tttgctgccc tgtttggcga tggcggagaa gaagcggttg
     2341 cccgcgctga ggcccacgca gtatacaaag agcgccagac ccagctgccc tacgccgtcg
     2401 ggaatggcca ggcggtagtg gccggcggcc agcgccacga aaagaacgcc ggaaaggccc
     2461 aggttcagtc cgtatatttt aatgctgccg agagcgatgc cgaaaaagat gattccaaag
     2521 agaaccaggg tgttgtgttg gagcaggata tcaaacataa agacggttag cggttttgtt
     2581 cccttccttg gcgcagagca ggggaatgcc cgtcacggat gagaatgcgt gcgttgagaa
     2641 agcggaggga gaggcgtggc aggtatcttc cagccctgtc agtttctccg caggtaatcc
     2701 agcagagtgg cctgatccag gcaggacata taaggcgtat ggtcgatgcc cttttccata
     2761 atgaacctgg tcagcagttt gcgggcggtc gtaaggagaa cggcgggatc ccagggcttt
     2821 tccagatagt tgtcaatgtg cgcgtcattg acggcctgga tagtgtccgc gtgggtggcc
     2881 tgcccggtca gcaggatttt gcgcgtcccg gcgaaacggt cgtctttggc gatgcctcca
     2941 agcaactcta ctcctgaacc tcccggcatg acgtggtcgg agataaccag tcccgtcagt
     3001 tctccgtcct ggtcgaattc ttccaacagt tcgcggcatt cggatgcgga ttccacccct
     3061 tccaggcgga agcaggaatt cagcggacga aggtcgcgca aaacgctgtc aaggacttcc
     3121 ggctggtcgt ccacgcaaat gatgttaatt ttttccatga ttgttgatgg ttggtatgat
     3181 tatcgtaaaa acggtatggc ccggcacgct ctccaggcgg atgcggccgc cgtagctgtc
     3241 cacaatgcgc cggacgatgg tgagccccag ccccaggccg aaggaaagcc ccttcttttt
     3301 ggtagtgaag ttgggacgga aaattttttc atgcaggtcc tttggaacgc cggggccgtt
     3361 atctgtgacg gagaccagaa tttcgcgggt ggtatgggat gtctggtcct tgctgctgat
     3421 tttttccacc cggcattcta cgtggatggc agggttgggg gtattttcta ttttcagcgc
     3481 gtcgcaggcg ttctgaagca ggttgatcca aatttgaacg agctccgtca tatccgcgaa
     3541 gatgccgggc acggagtccc tgggaggcag gaccatttca aaatcaacgc cgcgcagact
     3601 ggacttgacc agagcaacag cttccgtaag ggaatccgcc acgtttactc cttcttcccg
     3661 tctggtgttg cctcccccca gcaggcgaac ggaacggaca atggctgtag cgtgcctgac
     3721 agccagtttc atgtcatgca ggtcgtgacc caattcccaa taagggaggt tttgttccaa
     3781 ctggctgagg aatggtttgt ccagcagctc cggctgattc tgtatgtccg gaatcagggc
     3841 cagggccttg gccacgtcct gggaaagccc gtaatttttt tcaaactggc gcgccagcct
     3901 gcgctgttcg gaagtggatg cggaggaagc cccttccaac cctttgttgt acagggtgta
     3961 gcggaaggcg ttttcctggc ggaacatgtt gtccagggtt tccagaacaa agtctgtttt
     4021 acgggacagg actcccaggg aattgttgag ctcatgagct attccggcgg ataattgccc
     4081 cagggtggcg gccatttcag aacggtggag catccgcagc gcttcttcct tttccgcagt
     4141 gcggtcaaag acgcgggtat tgcgcgccgc cagttcatgg acaatggcgg gaacaaactg
     4201 ttccaggagc gtgccgtagt gttccacgtc cacggcaggg gtggcggtgt cgatataggc
     4261 cagttccaaa tcggtttccg caatgagatc
//
LOCUS       DAOMSU010000181         4285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1943_length_4285_cov_2.569267, whole genome shotgun sequence.
ACCESSION   DAOMSU010000181 DAOMSU010000000
VERSION     DAOMSU010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1943_length_4285_cov_2.569267"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PK077_07800"
     CDS             <1..390
                     /locus_tag="PK077_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRN24241.1"
                     /translation="EKAANGGNTPSSVYLGNIYAKGQGVAQDMERAMKWYEQAASAGD
                     AHSQYIVGLAYLEGSGVPVDEGKAFSWLRLAAGQDHVNAMLMLSVCYSTGKGTSQDAD
                     MAEVWKKKALQLNAEREGGSAPQTQKH"
     gene            complement(387..854)
                     /locus_tag="PK077_07805"
     CDS             complement(387..854)
                     /locus_tag="PK077_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24242.1"
                     /translation="MTHASPAPDEQEFWLFIQDAPLPAAEELQESMFRLDGCFRLNLS
                     GAPENAPDHVLEGEYLDEAGESQLDVFWVMETDDARQAFKRDGAALRQIGNRTKMLRF
                     ILEDEYALGHVFAMMHAILEHRDGLLVIPDSRGNVILERRKALDFLNREIRES"
     gene            967..1755
                     /gene="thiD"
                     /locus_tag="PK077_07810"
     CDS             967..1755
                     /gene="thiD"
                     /locus_tag="PK077_07810"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HRN24243.1"
                     /translation="MSIPVMMTIAGSDCSAGAGLQADLKAAHAMGAFALTAVTCVVSE
                     APGTVRGIQEVDPALVADQVRINLEHFPVRAVKTGMLYSPAIVRAVHEVLAGADVPVV
                     VDPVMIATAGDRLMREEAVAVYEELLLPGAALLTPNLDEAAVLLRSSVNPGRDELPEA
                     AARLAVRYGCPVLLKGGHLEGDCRDVLSGPDGCLLGQWERPRVQEVSTHGTGCSLSAA
                     VAARLAAGDGLVAAVERGLEFIASAIRNHLRWEQPVRVDALKLW"
     gene            1941..2213
                     /locus_tag="PK077_07815"
     CDS             1941..2213
                     /locus_tag="PK077_07815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24244.1"
                     /translation="MGQQIRKVTKRRRRIEYLKRKKEQSKLNSNAPIRLAVPVAKDAK
                     PAAKEAPAKKTAAKAPAKKTETKAAAAKKAPAKKPAAKKAAEPAAE"
     gene            2499..3881
                     /locus_tag="PK077_07820"
     CDS             2499..3881
                     /locus_tag="PK077_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HRN24245.1"
                     /translation="MRHRTADTPKGHRNYVIIACDVLLFLAMLKWLPVEPEVARGLAV
                     LTFIGILWLTEALHVTVTSLLVPVLAMFMGILPGEKALSGFADPTIFLFFGGFALAGA
                     LHEQKIDAWLAGKILRMARGSLGMALILIFLATAFLSMWMSNTATAAMMLPLVIGLLD
                     RVPAEKLKTTAPFAILGVAYSASIGGMGTLVGSPPNAIAAHELGMGFAEWFRIAMPMV
                     LVFGVIVFFLMYLFLRPNLGMHVDMVPEEREGTEGRLNARQVRVLILFVLVAGSWMCS
                     SFLSSALGGIPSMDTLIALSAAVLLPLCGVINWSGIAKNTDWGVLLLFGGGITLSSIL
                     VQTGAAGFLADQVSSLAMGHSPIVILLIISLFITSLTEFCSNTASAALVAPLMVTVAS
                     AMGMSATPLVLLVGIGASCAFMLPVSTPPNALAFATGRFPQMTMVKVGLLINVVLVFV
                     KAFWAWIFWM"
     assembly_gap    3955..3964
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3965..>4285)
                     /locus_tag="PK077_07825"
     CDS             complement(<3965..>4285)
                     /locus_tag="PK077_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRN24246.1"
                     /translation="AILIVEFAVLELRQGTDLLTATLDAARIRLRPILMTSIAFIMGC
                     LPLAVATGAGAAARQVVGVGVIGGMMTAVFIGVFFIPSFFYLIAKLAGLDKKAARKLQ
                     EKEQG"
BASE COUNT          751 a         1199 c         1346 g          979 t
ORIGIN      
        1 gagaaggccg ccaacggagg gaatacgcct tcgtccgttt atctgggaaa tatttatgca
       61 aaagggcagg gtgtggcgca ggatatggag cgtgccatga agtggtatga acaggcggct
      121 tcagccggag acgcccattc ccagtatata gtgggactgg cttatctgga aggttccggc
      181 gtgcctgtgg atgagggcaa ggcgttcagc tggctccggc tggccgcggg gcaggatcac
      241 gtcaatgcca tgctgatgct ttccgtctgt tacagcaccg gaaaggggac ttctcaggat
      301 gcggatatgg cggaagtctg gaaaaagaag gcgcttcaac tgaatgcgga gcgcgagggc
      361 ggttcagctc cgcagacgca gaaacattag ctttccctga tttccctgtt caggaaatcc
      421 aaagctttcc tccgttccag aataacgttc ccccgggagt ccggaatgac cagcaggccg
      481 tcccggtgtt ccagtatggc gtgcatcatg gcgaatacat ggcccagggc atattcgtct
      541 tccagaatga agcgcagcat tttggtgcgg ttccctatct gccggagggc cgcgccgtcc
      601 cttttgaacg cctggcgcgc gtcgtccgtt tccatgaccc agaatacgtc cagctgggat
      661 tcccccgctt catccagata ttctccttcc agtacatggt ccggagcgtt ttccggcgcg
      721 ccggaaaggt tcagccggaa acagccgtcc aggcggaaca tgctctcctg taattcctct
      781 gcggccggca gaggggcatc ctgaatgaaa agccagaatt cctgttcgtc cggggcgggg
      841 gaggcgtgcg tcatgagatg attatggcat tccctccttg ttttcccacc cggatttgtg
      901 acattgaggc ggcgcgtgcg gatttttgga tgttcaagag ctttcttccc gattaaaaac
      961 agggccatga gtattcccgt aatgatgacg attgccggtt ccgactgctc cgcgggggcc
     1021 gggctccagg ctgacctgaa agcggcacat gccatggggg cgtttgcgct tacggccgtc
     1081 acctgcgtcg tgtccgaggc gcccgggaca gtgcgcggca ttcaggaggt ggaccctgcg
     1141 ctggtggctg atcaggtgcg catcaatctg gaacattttc ccgtgcgcgc cgtgaagacg
     1201 ggcatgctgt attccccggc tatcgtccgt gcggtgcatg aggtgctggc cggggcggat
     1261 gtccccgttg tggtggaccc ggttatgatc gccacggcgg gagaccgcct gatgcgggaa
     1321 gaggcggtgg ccgtttacga agagcttctg ctgccgggag cggcgttgct tactcccaat
     1381 ttggatgagg ctgccgtcct gctccgttct tctgttaatc cgggcaggga tgaactgccg
     1441 gaggccgccg cgcgcctcgc cgtcaggtac ggatgccccg tccttctgaa gggcggccat
     1501 ctggaggggg actgccggga cgtgctgtcc ggcccggacg gctgcctgct ggggcaatgg
     1561 gagcgcccgc gcgtacagga ggtgagcact cacggaacgg gctgttccct gtctgccgcc
     1621 gtcgcggcgc ggctggccgc cggggatgga ctggttgcgg cggtggagcg cggcctggaa
     1681 ttcatcgctt ccgccatccg gaaccatctg cgctgggaac agccggtccg ggtggatgcg
     1741 ctgaaattgt ggtaaaacct ccttcgcccc ggttgtcttc ctctcccgca cggattgggg
     1801 aacccgggga ctgcctccgc ctttctgtgc cttaataacg ctttgtgctt gcgtatggga
     1861 ggtggggttg gtagttgtat ccacgcccat gtgccacatg gagcttttaa gaattagtaa
     1921 atatcatagc aattatcact atgggacagc aaatccgcaa agtcaccaag cgccgcagac
     1981 gtattgaata cctcaagcgt aagaaggaac agtccaagtt gaattccaat gcgcctatcc
     2041 gtctggccgt tcccgttgcc aaggatgcca agcctgccgc caaggaagct cccgccaaaa
     2101 agactgcggc caaggccccg gccaaaaaga ctgaaaccaa ggctgccgcc gccaagaagg
     2161 ctcccgccaa aaagccggct gccaagaagg ccgctgaacc cgccgccgaa taattgcccg
     2221 ggcggaggtt tcccctccgt gcatcaatca gacagtttta caagccgccg tgcgattgca
     2281 tggcggtttt ttttgcatgt ccgggcttcc tgtattttcc cggagcggga agaggtggcg
     2341 cggctttccc ggtgatgcgc gccgtgcgta ttctcaagcc ggcggttgat ccgtgtttcc
     2401 ctgtttccgg ggaaggcctt ttccggggtt ttatggaaaa gcattgacac tatttttctg
     2461 atgcgtacaa ataaaatagc gtcgtttatc tttactttat gcgccaccgt actgcagaca
     2521 ctcccaaagg gcaccgcaat tacgtcatca ttgcgtgtga cgtgcttctt tttctggcta
     2581 tgctcaagtg gcttcccgta gagccggagg tagccagggg gctggctgtt cttaccttta
     2641 tcggcatcct gtggctgacg gaagccctgc acgtaaccgt aacctccctg ctggtgccgg
     2701 tgcttgccat gtttatgggg attctgccgg gggagaaagc tctgtccggt tttgcggacc
     2761 ctaccatttt tctgtttttc gggggctttg ccctggccgg ggctcttcac gaacagaaaa
     2821 ttgatgcctg gctggcggga aaaatcctcc ggatggcccg cgggagcctg gggatggccc
     2881 tgattctgat tttcctggct acggcgtttc tttccatgtg gatgtccaat acggccacgg
     2941 cggcgatgat gcttcctctg gtgatcggcc ttctggaccg cgttccggcg gaaaaactta
     3001 agacgacggc tccttttgcc atcctggggg tggcgtacag cgcctccatc ggcggcatgg
     3061 ggacgctggt gggttctcct cccaacgcca ttgcggcgca tgagctaggc atggggtttg
     3121 cggaatggtt ccggattgcc atgcccatgg tcctggtgtt cggggtgatc gttttcttcc
     3181 tgatgtacct gttcctccgc ccgaatctgg ggatgcatgt ggatatggtg ccggaggaaa
     3241 gggagggaac ggagggcagg ttgaatgcca ggcaggtgcg tgtccttatt ctcttcgtgc
     3301 tggtggccgg gagctggatg tgcagcagtt tcctttcctc tgctttgggc ggcatcccgt
     3361 ccatggacac gctcatcgcg ctgagcgctg cggtgctgct gccgctgtgc ggggtgatca
     3421 actggagcgg catcgccaaa aatacggact ggggcgtcct gcttttgttc gggggcggca
     3481 tcacgctcag cagcatcctc gtccagacgg gggcggcggg attcctggca gaccaggtat
     3541 cttctctcgc catggggcac agccccatcg tcatcctgct catcatcagc ctcttcatca
     3601 cgtcgctgac ggaattctgt tccaatacgg ccagtgcggc cctggtggcc ccgctgatgg
     3661 tcacggtagc ctccgccatg ggcatgtctg ctacgccgct ggtgcttctg gtaggcatag
     3721 gcgcttcctg cgccttcatg ctccccgttt ccactcctcc taacgctctg gccttcgcga
     3781 cggggagatt ccctcagatg accatggtaa aggtggggct gctgattaat gtggtgctcg
     3841 tgttcgtgaa ggctttttgg gcctggatat tctggatgta ggaggaatgg gaaactgtgc
     3901 gggagccgtt ttcccctttc ggaagtgtca ttcggcctgc ttcgcggggg tgggnnnnnn
     3961 nnnntccctg ctccttttcc tgaagtttcc gcgccgcttt tttgtccagc ccggcgagct
     4021 tggcaatcag gtagaagaag gaaggaatga aaaatacccc gatgaagacg gctgtcatca
     4081 tgccgccgat gaccccgacg cctaccacct gccgcgccgc tgcgcccgct cccgtggcga
     4141 cggccagagg aaggcagccc atgataaagg cgatgctggt catcaggatg gggcgcaggc
     4201 ggattctggc cgcatccagg gtggccgtga gcaggtccgt gccctgacgg agttccagca
     4261 cggcgaattc cacgatgagg atggc
//
LOCUS       DAOMSU010000182         4260 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1957_length_4260_cov_2.719857, whole genome shotgun sequence.
ACCESSION   DAOMSU010000182 DAOMSU010000000
VERSION     DAOMSU010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1957_length_4260_cov_2.719857"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..946
                     /locus_tag="PK077_07830"
     CDS             <1..946
                     /locus_tag="PK077_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRN24247.1"
                     /translation="HGNPSKAMMPPVKIFLLLIFSWAAFPRPLQAREEPPASPSWPCC
                     VPARPGSRILFLGNSYTFFNRMPAMVKAMADTKGLRPDVHMHAAPAASLQSHWNDRRA
                     RSNMEGTAWDFVILQDQSATPVRAPERTMEFGEKWCSLVRAAKGTPILMLTWGKRGAS
                     GTPDPQEQKALLETYLKLARREKAALAPVGIAWENCLKRHPGIQLYQPDGRHPTEEGS
                     YLAACVIYFTLFGKSPLGLPGRLSLKGTRLSNLPADRARILQTVARDTCRQLFSATAG
                     TRPATAGLQAAALSPHSAKSTRKTGTGPLPWQRTYILL"
     gene            919..1872
                     /gene="trxB"
                     /locus_tag="PK077_07835"
     CDS             919..1872
                     /gene="trxB"
                     /locus_tag="PK077_07835"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRN24248.1"
                     /translation="MATDIYTIMSEQVIIIGAGCAGYTAAIYTARANLSPLLITGSQI
                     GGQLTTTTEVENFPGFPDGVMGPDLMFLMQQQAEKFGTRFAYEDVKSVVRDEATGLFT
                     VKTSGQNYETRSIIVATGASARYLGIPGEEGLVGHGLTACATCDGAFYRDVPVCVVGG
                     GDSACEEAMFLTRFASRVYLIHRRDTLRASKIMAERTLSNEKIFPMWNSTIVSYKTDD
                     KGELEAVMLKDVVEGDETELPVKCVFMAIGHTPNTSFLGDLVDRDDAGYIIRETGMMA
                     TRTPGLFAAGDVADPHYRQAISSAGMGCSAAIEAERYLLGL"
     gene            1943..3322
                     /locus_tag="PK077_07840"
     CDS             1943..3322
                     /locus_tag="PK077_07840"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HRN24249.1"
                     /translation="MHHYSIFELFSIGIGPSSSHTVGPMRAAHRFLEQIRHSPRLAEI
                     SGIRCECYGSLAATGHGHGTDTAIMLGLLGEEPHTVEPRSIPGKIARLHQQETLSVTE
                     EKNVRFSPSRDLDLSHYQPLRLHPNGMQFTAVNQDGDPVEDAVFYSTGGGFVSSEQEL
                     LHPEERDRETFPFPYESAEELLHMADERGCPLSSIVMANECAMLPEREVREKLDLIWA
                     TMKQSISNGMSARGNLPGVLQVPRRAKTLRKNLLVHGESALKDPLSIMDWINLYAIAV
                     SEENAAGGRIVTAPTNGAAGIVPAVLNYATKFCVSPYPDAVHRFLLTAGGIAILYKKN
                     ASISGADVGCQGEVGVACSMAAAALAEYLGGTPHQVENAAEIGMEHNLGLTCDPIAGL
                     VQIPCIERNAIAAIKAINAARMALGGTGAHFVPLDKVIRTMLDTGRDMQSKYKETAQG
                     GLAVNVVNC"
     gene            3424..4113
                     /locus_tag="PK077_07845"
     CDS             3424..4113
                     /locus_tag="PK077_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24250.1"
                     /translation="MLSRLFLALALLLGGTGLSRAVHVVMCGGPALRQWEGLRIQPDR
                     HDNWWANFVRASTIRIAQIQQQDPAARITWIVYRPGYATRGKEDGKPYTRWIADLASK
                     YKIRLVWVNSADQAIRALNSSPRFHGDKVESFYYFGHSNCYAWMLDYGNNIMAVSTEW
                     IHETDLNRIRRDIFTPQSDCWSFGCYTGASMSRWWKRIVGVPLWGNTESTRYGPVSDG
                     RLPEGAGKWVK"
BASE COUNT          925 a         1308 c         1208 g          819 t
ORIGIN      
        1 acatgggaac ccgtctaaag ccatgatgcc gcccgtcaag atattcctgc tgctgatttt
       61 ttcctgggca gctttccccc gccctctcca ggcccgggaa gagccgcccg cgtccccctc
      121 ctggccatgc tgtgttcctg cacgccccgg cagccgtatt ctctttctgg gaaacagcta
      181 tacgtttttc aaccgcatgc ccgccatggt taaagccatg gcagacacca aaggattgcg
      241 gccggacgtg cacatgcacg cggctccggc agcatccctc cagtcccatt ggaatgacag
      301 gcgtgcccgc agcaacatgg aaggaacggc atgggacttc gttattctcc aggaccaaag
      361 cgccacacct gtccgggctc cggaacgaac catggagttc ggagaaaaat ggtgctctct
      421 ggtacgagcc gccaaaggaa cgcccatcct gatgctgacg tgggggaaaa gaggagcttc
      481 cggaactccg gacccgcagg agcaaaaggc tcttcttgaa acgtatttga aacttgcccg
      541 gagggaaaaa gcagcgctcg cccccgtggg cattgcctgg gagaattgcc tcaaacgcca
      601 tccaggcatt cagctgtacc agcctgacgg ccggcacccg acggaagaag ggagctacct
      661 ggcggcatgc gtaatctact tcacgttatt cggaaagtct ccgctgggcc tgccgggccg
      721 cttgtccttg aaagggacgc gactgagcaa tcttcccgca gacagggcca gaatattgca
      781 gaccgtcgcc agggatacat gcaggcagct cttttccgcc actgccggaa cccgtcccgc
      841 aaccgccgga ttgcaggccg ctgcattgtc tccgcatagc gcaaaatcta ctcgcaaaac
      901 gggcacgggc cctttaccat ggcaacggac atatatacta ttatgagcga acaagtcatt
      961 atcatcggtg ccggctgcgc tggttacacg gcggccattt acaccgcccg cgccaatctc
     1021 tctccgcttc tgatcacggg ttcccaaatc ggcggacaac tgaccaccac gacggaagtg
     1081 gaaaacttcc ccggctttcc ggacggcgtg atggggccgg acctgatgtt cctgatgcag
     1141 cagcaggcgg aaaagttcgg cacccggttt gcatatgagg acgtcaagag cgtcgtcagg
     1201 gatgaagcca ccggactgtt caccgtgaag accagcggcc agaattacga aacccggagt
     1261 atcattgtgg ctacgggagc atccgcccgc tatctgggaa taccgggaga ggaagggctg
     1321 gttggccacg gactcaccgc ctgcgccacc tgtgacggag ctttttaccg ggacgtgcct
     1381 gtgtgcgtgg taggcggagg agacagcgcc tgtgaagaag ccatgttcct gacgcgcttc
     1441 gcttcccggg tgtacctgat ccaccgccgc gacacgctcc gcgcttccaa aatcatggcg
     1501 gaacgaacgc tgtccaatga aaagattttc cccatgtgga attccaccat cgtaagctat
     1561 aagacggacg acaagggaga actggaagcc gtcatgctga aggacgtagt agaaggggat
     1621 gaaacggaac ttcccgtcaa atgcgtcttt atggcgatcg ggcacacgcc gaacacttct
     1681 ttcctggggg atctggtgga ccgggacgac gccggctaca tcatccggga aacagggatg
     1741 atggcgacca ggacgccggg attgttcgcc gccggagacg tggcggatcc tcactaccgc
     1801 caggccattt cttccgccgg aatgggctgc agcgccgcga ttgaagcgga acgctacctg
     1861 ctggggctgt aaccccatct ttcggaacgg gccggaattc ccagcgggat ttccggccct
     1921 tccccgctac aggaaaacgg acatgcacca ttacagcatt tttgagcttt tcagcatcgg
     1981 cataggtcct tcatcctccc ataccgtagg gcccatgcgg gcggcgcacc gctttcttga
     2041 acagatacgc cactccccgc gcttggcgga aatctcaggc atccgctgcg aatgctacgg
     2101 ctccctggcc gccaccggac atgggcacgg cacagacaca gccatcatgc tggggctgct
     2161 gggagaagaa ccccatacgg tggagccgcg cagcattccc ggaaaaatcg cccgcctgca
     2221 ccagcaggaa acgttgtccg tcacggaaga aaaaaacgtc cgcttctctc cttcccgcga
     2281 cctggatctt tcccactacc agccccttcg cctgcatccg aacgggatgc agttcactgc
     2341 cgtcaaccag gacggagacc cggtggaaga cgcggtattt tattccacgg gaggcgggtt
     2401 cgtctcttcc gaacaggaac ttctgcatcc ggaagaaagg gaccgggaaa cctttccttt
     2461 tccttatgaa tccgcggagg aactgctgca catggctgat gagcgcggat gccctctttc
     2521 ctccattgtc atggcgaacg aatgcgccat gctcccggaa cgggaagtcc gtgaaaaact
     2581 ggacctaatc tgggcgacga tgaagcagtc catctccaac ggcatgagcg cgcgcggcaa
     2641 cctgccgggc gttctgcagg ttccgcgccg ggccaaaact ttgcgcaaga atcttctggt
     2701 gcacggagaa tcggccttga aagaccctct ctccattatg gactggatca atctgtacgc
     2761 cattgccgta agtgaggaaa acgcggccgg cggccgcatc gtcaccgctc cgaccaatgg
     2821 agcggcggga atcgttccgg ccgttcttaa ctacgccacc aaattctgcg tttccccgta
     2881 tccggatgcc gtgcatcgct tcctgctcac cgccggggga atcgccatcc tgtacaagaa
     2941 aaacgcctcc atttccggcg cggacgtagg ctgccaggga gaagtgggcg tagcctgctc
     3001 catggccgcc gccgctcttg cggagtacct ggggggaaca ccccaccagg tggaaaacgc
     3061 cgcggaaata ggcatggagc acaatctggg cctcacctgt gatcctattg cgggcctcgt
     3121 ccagataccc tgcattgagc gcaacgccat cgccgccatc aaggccatca acgccgcccg
     3181 catggccctg ggcggcacgg gcgctcattt tgtcccgctg gacaaggtca tccgcaccat
     3241 gctggacacc ggaagggaca tgcaatccaa atacaaggag accgcccagg gcggcttggc
     3301 cgtcaacgtg gtgaattgct gagggtacat gaaccccctc ccttgtcttt tcacgcgcgt
     3361 ctgttcccgc aaaaaacgct ccgccggatt gacagatacg gggagcaaag gtagggtgcg
     3421 ggcatgcttt cacgcctttt tctcgctctg gccctgttgc tgggcggcac gggactgtcc
     3481 cgggccgttc acgtcgtcat gtgcggcggc cccgcactca ggcaatggga agggctgaga
     3541 atacagcctg acaggcatga caactggtgg gccaactttg taagggcctc caccatccgc
     3601 atcgcccaga tccagcagca agatcctgcc gccagaatca cctggattgt ttaccgcccc
     3661 ggttacgcaa cccgggggaa ggaagacgga aaaccttaca cccgctggat tgcggacctg
     3721 gcttccaagt ataaaatcag gctcgtctgg gtaaacagcg ccgaccaggc catccgggcg
     3781 ctgaattcct ccccgcgctt ccacggcgac aaggtggaat ccttttacta cttcggccat
     3841 tccaactgct atgcgtggat gctggactac ggcaacaata tcatggccgt ttccacggaa
     3901 tggatccatg agacggacct gaacaggata cggcgggata ttttcacgcc gcagtccgac
     3961 tgctggagct tcggctgcta tacgggagcc agcatgtccc gctggtggaa acgcatcgta
     4021 ggcgtccccc tctggggcaa cacggaatcc acccgctacg gccccgtctc tgacggcagg
     4081 ctgccggaag gcgcgggaaa atgggttaaa tagcaggctt gcaccgctct ttcctttaaa
     4141 gacaggctcc ctaaaatcgg gagcctgttt tgttgccttt catggaaagt gaggatatgc
     4201 cggattttca atccggaagt caagcgtaaa ttctgataac caagaatttg tgccgaactt
//
LOCUS       DAOMSU010000183         4225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_1987_length_4225_cov_2.910312, whole genome shotgun sequence.
ACCESSION   DAOMSU010000183 DAOMSU010000000
VERSION     DAOMSU010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1987_length_4225_cov_2.910312"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..413)
                     /locus_tag="PK077_07850"
     CDS             complement(<1..413)
                     /locus_tag="PK077_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24251.1"
                     /translation="MPPRRPESILPGMAHALTLLVFSACLGACAPGIPEIPPPASSVT
                     RREALAASRAYTSMIWRGSLRNVRHGTDGDGIRTDTPDASAAGDDAGAWWKPGMRSIG
                     MPYKWGGFDTPRQFSERLKADAANGGLPAAAGDMGT"
     gene            565..966
                     /locus_tag="PK077_07855"
     CDS             565..966
                     /locus_tag="PK077_07855"
                     /inference="COORDINATES: protein motif:HMM:NF021221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HRN24252.1"
                     /translation="MDEEQQIIQEPVQEPAPEKQSDKTQAILTAVSPLVSVFFGGSAG
                     VNIIPPLICWLVWKDANSLVDKVGKNILNAQISWAIYTVAACLSCLILIGFVLAPVVV
                     IAWIVFSIIAAVKAANGNYDYVAPFTIKFLK"
     gene            complement(1063..1977)
                     /locus_tag="PK077_07860"
     CDS             complement(1063..1977)
                     /locus_tag="PK077_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24253.1"
                     /translation="MMPLSASSMNRVSYMCPVTISNDVVQGQTDIQDSLTVDSGGNLY
                     IINAPVYVGGPNQPDHGHRTAHLVIRNGGAMTLLGNLPDHMTVFLGDKANGSLEINGG
                     RLLMGQGRIQGAREHEGRIAMTDGWLFASEVDLPAEGSELVIRHGLMRIRKLSGNAST
                     RIYGGVLHVKEEARASRIHLIDDGVLLLGSVTSQPSADVMAGAGINFRGDGGALVIRI
                     PHPENALTRTREAEHVFDELLRRGKLFHDSEPMTSFQGFHMREFTGHDGLAYAALRPS
                     AQLDAEQNQVTRLLHTFMYGGSEKDMPI"
     gene            complement(2135..>2422)
                     /locus_tag="PK077_07865"
     CDS             complement(2135..>2422)
                     /locus_tag="PK077_07865"
                     /inference="COORDINATES: protein motif:HMM:NF013334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HRN24254.1"
                     /translation="EKRGGNRLRRVYMTIGSLSGVVPEALQSAFLALKDSYAAQDADL
                     DVAWVEAVCRCDACRADFSFTEHGYICPGCGEPALAILRGQELELTRVEWE"
     assembly_gap    2423..2432
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2467..>2680)
                     /locus_tag="PK077_07870"
     CDS             complement(2467..>2680)
                     /locus_tag="PK077_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24255.1"
                     /translation="IGMKEVLGAAQLCGTLPRAITLVGVEALHTDFCRPMSAEVQEAL
                     TKALDTAEALIARALAEHQARKNAHA"
     assembly_gap    2681..2690
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2691..3021)
                     /locus_tag="PK077_07875"
     CDS             complement(<2691..3021)
                     /locus_tag="PK077_07875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HRN24256.1"
                     /translation="MKLEPYTEELRGCPVKGNAYPVLVLGIGNPLMGDDGAGVELAHR
                     LQERDYGPLVHVEEGGTQGMTLLPLLEDADTLILLDAVRTGALPGTVVTRSREELPRH
                     FSRVMSPH"
     gene            complement(3043..>4225)
                     /locus_tag="PK077_07880"
     CDS             complement(3043..>4225)
                     /locus_tag="PK077_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HRN24257.1"
                     /translation="TGPVKTTLIDNDLTLAVEDAIPASPVYAPEEILALAVCASPNGS
                     RDAGDLALLHAARERGITLPYAQRENSWEAPSRERPYSTAVLKAADKADASPFMVARG
                     NLKALEKLCEVSSLEKERMNKAFEEHSPSGFEPVAVAVHRSGAPWRLLGVVPMHAMRD
                     VRRLSMAKANFRYFHVWDWPLRVLHWTWVLCIIGLSATGICIAEGWFLKMGDLHGAFQ
                     FGTLRFVHYALGWILVVVMMLRFSCFFMASNKYQSFRALFPVSRQEWKDLYATAVDYL
                     FARSYDGPRYIGHNPLQQWTYTGVYVLFTTMVVTGLALYALYEPRHWFYRWFMPLNDL
                     IGIPYVRLVHLIGMWCFIIFAMIHVYLSILSGNVDRDGTISSMFSGGRWLRKGVKFRD
                     E"
BASE COUNT          793 a         1245 c         1305 g          862 t
ORIGIN      
        1 gtgcccatat ctccggccgc ggcgggaagc cctccgttgg cggcatccgc cttcaggcgt
       61 tcggagaact gccgcggggt gtcgaagccg ccccatttat acggcatgcc gatggaacgc
      121 atgcccggct tccaccatgc tccggcatca tctccggctg cggaggcgtc cggcgtgtct
      181 gtccggatgc cgtccccgtc cgttccgtgg cggacattcc ggagagagcc ccgccaaatc
      241 atggaggtat aggcccggct ggctgccagg gcttcacggc gggtgacgga agaagcggga
      301 ggcggaatct ccggtattcc cggagcacag gcccccaggc aggcggagaa gacgagcagc
      361 gtcagggcat gggccatgcc cggcagtatg gattccggcc tgcgtggcgg caaggatgat
      421 tgccggagag cgttttttat tcaggggacg gacggtctgg aagcttgact acggggagga
      481 atatgtaaaa aaggtctcat caaggcggaa tgaaacggaa aaggtttccg ttcaccttca
      541 tccgctttcc atctctttat ttttatggac gaagaacaac aaatcatcca ggaaccggtt
      601 caggagcccg cccctgagaa acaatcggat aaaacgcagg ctatcctgac ggcggtttct
      661 ccgctggtgt ctgtcttttt cggaggctcc gccggcgtga acattattcc gcccctgatc
      721 tgctggctgg tctggaagga tgccaactct ctggtggaca aagtaggaaa gaatattctg
      781 aatgcccaga tttcctgggc gatttatacg gttgcggcct gtctttcatg tttgatcctg
      841 atcgggttcg tgctcgcccc cgtggtagtc atcgcttgga tcgttttctc cattattgct
      901 gccgtcaagg cggcgaacgg gaattacgat tatgtggctc ccttcacgat taaattcctg
      961 aaataacgtt tttccgtttt tcagcagatc aacagatgaa aaaaccgccg gtacggaatg
     1021 ccggcggttt tttgatgagg gctttccatt tgccggaggg gttcagatgg gcatgtcctt
     1081 ttcactgccg ccgtacatga acgtatggag caggcgggta acctgattct gttccgcgtc
     1141 cagctgggcg gaggggcgca gcgcggcgta cgccagtccg tcatgccccg tgaactccct
     1201 catatggaac ccctggaagc tggtcatggg ttcactgtca tgaaagagct tgcccctacg
     1261 gagcagttcg tcaaagacat gttccgcttc ccgcgtgcgc gtcagagcgt tttccgggtg
     1321 ggggatgcgg attaccagcg ctccgccgtc cccgcggaag ttgatgcccg ctccggccat
     1381 gacatccgcg gagggctgac tggtgacgct gcccagcagc agcacgccgt cgtctatcag
     1441 atggatgcgg ctggcgcggg cttcctcctt tacgtgcaga acgcctccgt aaatgcgggt
     1501 ggatgcattg ccggagagtt ttctgatacg catcaggccg tgcctgatga cgagttccga
     1561 gccctccgcc ggcagatcca cttccgaggc gaagagccag ccgtccgtca tggcgattct
     1621 gccttcatgc tccctggcgc cctgaatgcg cccctggccc atcagaaggc gaccgccgtt
     1681 gatttccagg ctgccgttgg ccttgtcccc caggaaaacg gtcatatggt ccggcaggtt
     1741 gcccaagagc gtcatggcgc ctccgttccg gatgacgagg tgggcggtgc ggtgcccgtg
     1801 gtccggctgg ttggggccgc ccacgtaaac aggagcgttg atgatgtaaa gatttccgcc
     1861 ggaatcaacg gtaagggaat cctgaatgtc cgtctggccc tgcactacat cattgctgat
     1921 ggtgaccggg cacatgtagg agacacggtt catggatgag gctgaaagcg gcatcatccc
     1981 tgttagaatg aatgggttga tattgctcat ggctctggtt acatagttgg tttgacgttt
     2041 gaagcgcgat ggatgcgctc caatggtatt agaccatcca tccggggaaa gtgttcgcgt
     2101 aaatttccct tatttttccc ggaaggggca ctgttcattc ccactccaca cgggtcagct
     2161 ccagttcctg cccgcgcagg atggccaggg ccggttctcc gcagccgggg cagatgtagc
     2221 cgtgttccgt aaaggaaaaa tccgcccggc atgcgtcaca gcggcacacg gcctccaccc
     2281 aggcgacgtc caggtctgca tcctgcgccg catacgagtc tttcagggcc aggaaggcgg
     2341 attgaagggc ttccggcacc acgccgctca ggctgccgat ggtcatgtac acgcgcctca
     2401 gcctgttccc tccgcgtttt tcnnnnnnnn nnggccatgt ccagcgcgcc ttccatgatg
     2461 cctacttcat gcatgggcgt ttttccgggc ctgatgttcc gccagggcgc gggcgatgag
     2521 ggcctccgcc gtgtccagcg ccttcgtcaa ggcttcctgc acttccgcgg acatggggcg
     2581 gcagaaatcc gtatgcaggg cctccactcc caccagcgtg atggcgcgcg gcagggtgcc
     2641 gcacagctgg gccgccccca gcacttcctt catgcctatc nnnnnnnnnn ggtggggaga
     2701 cataacgcgg gaaaagtggc gcggcagttc ttcccggctg cgcgtgacga cggttcccgg
     2761 cagggccccc gtcctgacgg cgtccagcag gatgagcgtg tccgcatctt ccagcagggg
     2821 aagcagggtc atgccctgcg ttcccccttc ctccacgtgg accagcgggc cgtagtcccg
     2881 ttcctggagg cggtgggcca gctccacgcc tgctccgtcg tcccccatga gcgggtttcc
     2941 gatgcccagc acgagcacgg gataggcatt tcccttcacg gggcagcccc gcagttcttc
     3001 cgtgtatggc tccaatttca tgtcaagggg agggcggaga ggttattcat cccggaattt
     3061 gacgcccttg cgcagccatc gtccgccgct gaacatggag gagatggtgc cgtcccggtc
     3121 cacgttcccg gaaaggatgg aaaggtacac gtggatcatg gcgaagatga tgaagcacca
     3181 catgccgatc aaatgcacca ggcgcacgta cggaatgcct atcaagtcgt tcagcggcat
     3241 gaaccaacgg tagaaccagt ggcgcggctc atacagggca tacagggcca gcccggtcac
     3301 caccatggtg gtgaacagga cgtacacgcc cgtgtatgtc cactgctgca ggggattgtg
     3361 gccgatgtag cgggggccgt cataactgcg ggcgaacagg tagtccaccg ctgtggcgta
     3421 caaatccttc cactcctgtc tggaaacggg gaacagggcc cggaaactct ggtatttgtt
     3481 ggaggccatg aagaagcagg aaaaacgcag catcatcacc accaccagaa tccaccccag
     3541 cgcgtaatgc acgaagcgga gcgttccgaa ttggaaagcc ccgtgcagat cccccatttt
     3601 caggaaccac ccttccgcga tgcagatgcc tgtggcggac aggccgatga tgcagagcac
     3661 ccacgtccag tgcagcacgc gcagaggcca gtcccagacg tggaaatagc ggaaattggc
     3721 cttggccatg gagaggcggc gcacgtccct catggcgtgc atgggcacga cgcccaacag
     3781 gcgccacggc gcgccggacc ggtggacggc cacggccacg ggttcaaagc cggaggggga
     3841 atgctcttca aaggccttgt tcatgcgctc cttttccagg gagctgactt cgcacagttt
     3901 ttccagcgcc ttcaggttgc cgcgggctac catgaaaggg gaggcgtccg ccttgtctgc
     3961 ggctttcaga acagccgtgc tgtacggcct ttcccgggag ggagcctccc aggagttttc
     4021 ccgctgggcg taagggaggg ttatgccgcg ttcacgcgcg gcatggagca gggccaggtc
     4081 tcccgcgtcc ctgctgccgt tgggggaggc gcacacggcc agagccagga tttcttccgg
     4141 agcgtacacc ggactggccg gaatggcatc ctccaccgcc agggtgaggt cgttatcgat
     4201 aagagtcgtt ttcacgggtc ctgtc
//
LOCUS       DAOMSU010000184         4200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2010_length_4200_cov_2.393245, whole genome shotgun sequence.
ACCESSION   DAOMSU010000184 DAOMSU010000000
VERSION     DAOMSU010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2010_length_4200_cov_2.393245"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..875)
                     /locus_tag="PK077_07885"
     CDS             complement(<1..875)
                     /locus_tag="PK077_07885"
                     /inference="COORDINATES: protein motif:HMM:NF040129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HRN24258.1"
                     /translation="MSEALQGAVGGSTGEAVKGLKGMDQEGRKACKWLNAGLINMSAT
                     ITAVGAGINGLRAGWGKFSAMLAGGDDLERVTRRMEAFTGGASSAAEAARDVVDFADT
                     PPFGLEETQRAAQLLLGCGVRASELKSTLEALGNVAAGGGMSLEQIGIRLSKAFQTGR
                     VTMEVLEPLMNSGINVMGVLGQRTGKTRAELQKMMTEGTIGFRDLKGALVSMGSAGGQ
                     FAGAMEKNTQDIENRVETLKGKVGALSRVFAEPVTSGIKDAMDSIGASWAGHGPEVER
                     GLRKTGELLGEIVKAG"
     gene            1064..1201
                     /locus_tag="PK077_07890"
     CDS             1064..1201
                     /locus_tag="PK077_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24259.1"
                     /translation="MIIFIIGIIFLLGFFLPLLEILTDPASWAIILIPILIFWLICHK
                     K"
     gene            complement(1212..1427)
                     /locus_tag="PK077_07895"
     CDS             complement(1212..1427)
                     /locus_tag="PK077_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24260.1"
                     /translation="MLMTVARATGWPEREILWEIPLARLVQYVHAVWSYDATPCRWSC
                     YTEPSGHVGDVLEQAREAWRKQVEGLE"
     gene            complement(1421..1918)
                     /locus_tag="PK077_07900"
     CDS             complement(1421..1918)
                     /locus_tag="PK077_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24261.1"
                     /translation="MELQEHERRALTEAALIGGNEFRWKSYRLRCMTLGSMLQLQRIG
                     NPYSRLGEINLGPDENGRHPSMWEALGVTDKAQIVYYLAEFLWVHMGNREEVREGVFA
                     PEEDRRALVEAAAMNIPGRDLVELECAVLGDIEVIQAGIVNPEPEGEDEEDPLGRGRP
                     GARPC"
     gene            complement(1948..2424)
                     /locus_tag="PK077_07905"
     CDS             complement(1948..2424)
                     /locus_tag="PK077_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24262.1"
                     /translation="MMNAADNKKLESDVVVFTENASRYETENTMLAALLLTLGVNMKC
                     TSGSVLIGSGARLSAPGGVITWQFEPKSEDGRFRTEEVIKLFGDKNWLADPENESPLA
                     YVACAFHNYKRLLDFVKSQVPLAVIRKGKRKALVRLDADPYWQGVAEGFLGGRPLI"
     gene            complement(2454..2804)
                     /locus_tag="PK077_07910"
     CDS             complement(2454..2804)
                     /locus_tag="PK077_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24263.1"
                     /translation="MPKYGIDSPEQGIFVESIDFDGQQEIYEQKNNVGKKCGLVIVDE
                     ELSFSMSGAVLSTGSSSLKMGGTLALANEIPQIWHTPPTGTTVFLKRSLKNTDAQKMD
                     VSGTVYGFGSSSAV"
     gene            complement(2880..3344)
                     /locus_tag="PK077_07915"
     CDS             complement(2880..3344)
                     /locus_tag="PK077_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24264.1"
                     /translation="MMTQADCLIKAVIACLEARFQEDRGNTERGIPDGFPVPLKMAVD
                     EDREGKEYALFQAAEMEEIVAGYCTYHAGISVDLHLDANDRTADEIRMLQAWMEERLK
                     EVDRAGLNAVESLRPYRNFLVIGKVRLGPAQDAAAEEGAFAVTWKMTVPVQF"
     gene            complement(3341..4117)
                     /locus_tag="PK077_07920"
     CDS             complement(3341..4117)
                     /locus_tag="PK077_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24265.1"
                     /translation="MANVKYKVDISRVLEKLEAAKRVGADGIRELTLEYAKRAASKAI
                     RTTPPNSLKNGGNGKRALEEHIARDIGGDPLETDVRLKRGEDGRLVPYAYPRKKRDGV
                     LLGVRGKKFKGIATVSADAFLRSHTLLKMGRKSSVRVLKGGGLMSPGVAQAGDVRRAL
                     AERRRHVGRMAAGWLRGAQVAGLKKVPAWIARHASHYDGAASLTVQGGRVRFEMENCP
                     EYPDRGQLSRVAAYALNSAGRDMRKVIKGYVAKLKKELNS"
BASE COUNT          826 a         1505 c          867 g         1002 t
ORIGIN      
        1 cccgctttca cgatttcccc cagcaattcg cccgttttcc tcaagccgcg ctccacctcc
       61 ggcccatgac cggcccatga cgcgcctatg gagtccatgg catccttgat gccgcttgtt
      121 accggttcag caaaaacccg gctcaaggcc ccaaccttgc ctttcagggt ctccaccctg
      181 ttctcaatgt cctgcgtatt tttctccatg gctcccgcaa actgccctcc ggcagatccc
      241 atggagacta aagccccctt caaatcccgg aaaccaattg ttccctccgt catcatcttt
      301 tgcaattccg ccctggtctt tcctgttcgt tgacccagca cgcccataac gttaataccg
      361 ctattcatta gcggctccaa aacttccata gttacgcggc cagtttggaa agctttggaa
      421 agacggatgc ctatttgctc caaactcatt ccgccgcccg cagccacatt accaagagcc
      481 tccaatgtgc tttttaactc gctcgccctg acgccgcaac caagaagcaa ctgcgccgcc
      541 cgttgcgttt cctccagccc gaacggaggc gtatcagcaa aatccaccac atcccgcgcc
      601 gcttccgccg cgcttgacgc gccgcccgtg aatgcctcca tgcgccgggt tactctctct
      661 aggtcatccc cgcccgccag catggcggaa aacttgcccc agcctgcccg caggccgtta
      721 atgccggccc ctaccgccgt aatagtggca ctcatattga taagccccgc attcagccat
      781 ttacacgcct tccggccctc ctgatccatg cccttcagcc ccttcacggc ctcccctgtg
      841 cttccgccca cggccccctg taaggcttcg gacatgccgc ttgccgctct cttactttcc
      901 tccgttgcgg caataaagcc gctcgcatca ccatctattt taataactgc gccttcgctc
      961 ataatatttt acattgactg ataatataaa aaataattaa atggacttcg tgttttcctc
     1021 catcatggaa atcttgctgt atctgttaca aaaaacaggt cttgtgatca tcttcattat
     1081 cggaatcatt ttccttcttg gcttcttcct gcctcttctg gaaatactga cagatccggc
     1141 aagctgggct atcatcctta ttcccatcct tattttctgg ctcatctgcc acaaaaaatg
     1201 aagaggccgg atcactccag cccctccacc tgttttctcc acgcttcccg ggcctgctcc
     1261 agcacgtccc ccacatgccc ggagggttcc gtgtagcagc tccaccggca cggcgtcgcg
     1321 tcatagctcc agaccgcatg cacgtactgc accagccgcg ccagcggaat ttcccacaga
     1381 atttcccgct ccggccagcc cgtggcacgc gccaccgtca tcagcatggc cgcgccccag
     1441 gacggccacg ccctaaaggg tcctcttcat cctccccttc cggctccgga ttcactatgc
     1501 ccgcctgaat cacttctata tcccccagca cggcgcattc cagttccacc aaatcccggc
     1561 cgggaatgtt catagcagcc gcttccacca gggcgcgccg gtcttcctcc ggcgcaaaaa
     1621 ccccttccct gacttcctcc cggtttccca tgtggaccca caggaattcc gccagataat
     1681 agacaatttg cgccttgtcg gttacgccca gggcttccca catggacgga tgccgcccgt
     1741 tttcatctgg gcccaggtta atttccccca agcggctgta aggattcccg atgcgctgca
     1801 actgcagcat gctccccagg gtcatacacc gcagccggta gctcttccag cggaattcat
     1861 ttcccccgat caacgccgct tccgtcaggg cgcgcctttc atgttcctgt agttccatct
     1921 ttcttgcttt tttggttgtc aattaagtta gattaaaggc cggccgccaa gaaaaccctc
     1981 cgccacgccc tgccaatacg gatccgcatc caaccgcacc agggccttcc ttttcccctt
     2041 gcggatgacg gcaagcggca cctggctttt cacaaaatcc agcagccgct tgtaattatg
     2101 gaacgcgcac gccacgtaag ccagcgggct ttcattttcc gggtcagcca gccaattctt
     2161 atccccgaaa agcttgatta cttcctccgt cctaaacctt ccatcttcgc ttttcggctc
     2221 aaattgccag gtaatcaccc cgcccggcgc ggaaaggcgc gcgccgctgc ctatcagcac
     2281 gcttccggac gtgcatttca tgtttactcc cagcgtcagc agcaacgcgg caagcatggt
     2341 gttttccgtt tcgtatctgg aggcgttttc ggtaaaaact accacatcac tttccagttt
     2401 tttattgtct gcggcattca tcaatcttac tatctaactt ttttattcag gcttcagaca
     2461 gccgaagacg acccgaaccc gtaaacagtg ccgctcacgt ccatcttctg cgcgtccgtg
     2521 ttcttcaaac tgcgcttcag gaagaccgtg gttccggtgg gaggggtgtg ccaaatctgc
     2581 ggaatttcat tggcaagcgc cagcgtcccc cccatcttca aagatgaaga gccggttgat
     2641 aaaaccgcgc cggacatgga aaaagaaagt tcttcatcta caatcaccaa accacacttt
     2701 ttcccaacat tgtttttctg ttcataaatt tcctgttggc cgtcaaagtc gatcgactca
     2761 acaaaaatac cctgttccgg cgaatcaata ccgtatttgg gcacgtctcc aatatgtgca
     2821 ggcatataat ctatttcctt tcgtgttaga gttcatctat ctaccattca ggagcttttt
     2881 caaaactgca cgggcaccgt cattttccaa gtcaccgcaa acgcgccttc ctccgccgcc
     2941 gcatcctgcg cgggccccag ccttaccttg cctatgacca ggaaattccg atagggccgc
     3001 aggctctcca cggcattcag cccggcgcgg tccacttcct tcagccgctc ttccatccac
     3061 gcctgcaaca tccgtatttc atccgccgtc cggtcattgg cgtccaaatg caaatccacg
     3121 gatattccgg cgtgatacgt acagtacccg gccacaattt cctccatttc cgccgcctgg
     3181 aacaacgcat attccttccc ttcccggtct tcgtccaccg ccatcttcaa cggcaccggg
     3241 aacccgtccg gaatcccccg ttccgtgttc cccctgtctt cctgaaaacg cgcctccagg
     3301 cacgcaatca ccgccttaat caaacaatct gcctgtgtca tcatgaatta agctcctttt
     3361 tcaacttggc cacatacccc ttgattacct tccgcatatc ccggcccgca gaattcagcg
     3421 catacgccgc cacgcgggaa agctgccccc ggtcaggata ttccgggcaa ttctccatct
     3481 caaaccgcac ccggccgccc tgaaccgtca aagaagccgc gccgtcataa tgggaggcgt
     3541 gccgcgcgat ccacgcgggc accttcttca gcccggccac ctgcgcgccc cgcagccacc
     3601 cggccgccat cctccccacg tgccgccgcc gctccgccaa agcccttcgc acgtctcccg
     3661 cctgcgccac tcccggagac atcaggccgc cgcccttcag cacgcgcacg ctgcttttcc
     3721 ggcccatttt cagcagggta tggctccgca aaaacgcatc cgcggaaacg gtggctatgc
     3781 ccttaaactt cttcccgcgc acccccagca acaccccgtc gcgcttcttc cgggggtaag
     3841 catagggcac cagccttcca tcctctccac gcttcagcct tacatccgtt tccaacggat
     3901 ccccgccaat atcccgcgca atatgttcct ccaacgctct ttttccgttt ccgccattct
     3961 tcaggctgtt tggcggcgtg gtgcgtatgg ccttgcttgc ggcccgcttg gcatattcaa
     4021 gggtcaattc cctgatgccg tcagccccca ccctttttgc ggcctccaac ttttctagca
     4081 cgcgggaaat atccacctta tacttgacat tcgccatttt tttgcccctt cccgctacac
     4141 ttcgctcaac tccagcacca gggccacgtc tccggcccag tcccgcaccc gcgcaatgcg
//
LOCUS       DAOMSU010000185         4195 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2016_length_4195_cov_2.136957, whole genome shotgun sequence.
ACCESSION   DAOMSU010000185 DAOMSU010000000
VERSION     DAOMSU010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2016_length_4195_cov_2.136957"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(188..1489)
                     /locus_tag="PK077_07925"
     CDS             complement(188..1489)
                     /locus_tag="PK077_07925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HRN24266.1"
                     /translation="MNDFRNSRDSRPRPVYRDRRSQPGRPRTAPKKNLFANKSPEGSE
                     QWLKPWVELKYFTYNPAVFPRMLGSVSGEIAPGSLVNVYDKNGELFGAGFWNEYSRTP
                     LRMVHHGKDAFAERDLDAALERAVKLRREVLRLDETTNAYRVLHGDSDGLGGLVVDRY
                     ADVLSLEVSTLAVWQRLDRWLPLLHRLCGTKRHVVQVDEGIARMEGIRAEAAPASPAP
                     VRLVKIVENGITYEVDFAQGHKTGFFCDQRDNRLKFASLVKGATVLDLCCYSGGFSIA
                     AKMLGGAAEVTAVDLDEKAVAMAKRNGNINRQRIDFVHADAFVYARQMVRNGRLFDAV
                     LLDPPKFIIGRDGYEEGIKKYHDLNMLGLQCVRPGGLFVTCSCSGLLSPAEFEHTVIK
                     AAQRQGRKLQIMAMTGPGWDHPFLSTYPEGRYLKVLWAIAL"
     gene            complement(1486..2391)
                     /locus_tag="PK077_07930"
     CDS             complement(1486..2391)
                     /locus_tag="PK077_07930"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRN24267.1"
                     /translation="MYRTLVFGADGRVGSRLAAYLEEHGLEVVKCGRGFCDLKDLSAL
                     KRVLLSSGATHVVNCAAVSGLEACLDDPETAHRVNAMAPEMMARICRLEGMRFIHLST
                     DYVLDGRREGLKTEEGKCRPVNVYGESKLEAEFRVREEMPDALVARVSWVFGNPGRPS
                     FPEMVLRRAMKREPLAAVADKWSMPTWVEDLCVWLRFLAYESGASGVLHLCQSGEPVS
                     WHSYAVTVLKCAVKHGLLPALPPVAEQKLDEQAGFRDARPRHTAMSCERLSSLMKQPV
                     RAYEEAIDMAVARYAADPTFLSSIS"
     gene            2483..3433
                     /locus_tag="PK077_07935"
     CDS             2483..3433
                     /locus_tag="PK077_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24268.1"
                     /translation="MFYHEYSLTACGPLNARAAEVCRKGALIKTDEGGYGCIQPWPEL
                     GDATLQEELDALRKGRPLPLGIRAAECARTDGEARAKGVSLFDGLHIPESHATLPSCV
                     SPATIRIMESKGFKAGKIKASANLAASLERLTMLASMVPSWRWRLDFNGCLNENDALK
                     FWKSLPHHLKTRIDFIEDPCPFSIQSWERLVDAGMPLALDMGSDVEHQPAISSDLPII
                     RIVKPAREATPEYLYEPPVFTTVMDHPVGQLWAVYQAAEYYRNFLPTEIPLCGLCTHL
                     LFEPDPFIDQMGGMNPQAAVPGGTGLGFDELLENIPWKIL"
     gene            complement(3700..>4195)
                     /locus_tag="PK077_07940"
     CDS             complement(3700..>4195)
                     /locus_tag="PK077_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24269.1"
                     /translation="GATIIFKHYCDFPTLFNGAKIGAYPHKNHTYYAQTWIYSPKAQT
                     VPFWISGHTWATSDWRNGPASVPGKWFHADPKFFVNGREIAPPQWKKPRNSGVLVDEN
                     YHFREPSMVPLKKGWNRVLVKSPSNNSARRWMFTFVPVLVNPKTPGCNVKEYPGLKFS
                     TRPE"
BASE COUNT          896 a         1336 c         1136 g          827 t
ORIGIN      
        1 ggcaccgcgg gacacggggt ccaggtcggc gcgcccggcc agccacgggg tcagctcccc
       61 ggccgtaaaa ctgagccgct gcaatacttg ttttgccata ttccccacca cgaaagcaga
      121 gaggggcaaa aaacgtcaac acgcctgaaa ggtttttccg aaatgcatcc cgggcctcaa
      181 cctccagcta cagggcaatc gcccacagaa ccttcagata acggccctcc ggataagtgc
      241 tcaggaaagg gtggtcccag ccggggccgg tcatcgccat aatctggagc ttgcgcccct
      301 gccgctgggc ggccttgatg acggtatgct caaactccgc gggggaaagc agcccggaac
      361 aggaacaggt gacaaacagc ccgccggggc gcacgcattg cagccccaac atattgaggt
      421 catggtattt cttgataccc tcttcatatc cgtccctgcc gatgatgaat ttgggcgggt
      481 ccagcagcac ggcgtcaaac aggcggccgt tgcgcaccat ctggcgcgca tagacaaagg
      541 catccgcatg cacgaagtca atgcgctgac ggttgatatt tccgtttctc ttagccatgg
      601 cgacggcttt ctcatccaaa tccacagccg ttacctccgc cgctccgccc agcatcttgg
      661 cggcaatgga aaagccgccg ctgtagcagc acaggtccag caccgtagcc cccttcacca
      721 gggaggcgaa cttgaggcgg ttgtcccgct ggtcacagaa aaagcccgtc ttgtgcccct
      781 gggcgaagtc cacttcatag gtgatgccgt tttccacaat cttcaccagc ctgacgggag
      841 ccggggatgc cggcgccgcc tccgcccgga taccctccat gcgggcaatg ccctcatcca
      901 cctgcaccac atggcgcttt gtgccgcaca gacggtgcag caggggcagc cagcggtcca
      961 gcctctgcca gacggccaga gtactcacct ccaggctcag cacgtccgcg taacggtcca
     1021 ccaccaggcc gcccaggccg tcggaatctc cgtgcagcac acggtacgca ttcgtggtct
     1081 catccaggcg cagaacttcc cggcgcagct tcacggcgcg ttccagcgcg gcatccagat
     1141 cccgttcggc aaaagcgtcc ttcccgtggt gcaccatgcg cagaggcgtg cggctgtact
     1201 cattccagaa tccggcgccg aacaactcac cgttcttgtc atagacgttt accaggctac
     1261 ccggcgctat ttccccgctt acggagccga gcatgcgggg gaacacggcc ggattgtatg
     1321 tgaaatactt cagctccacc cagggcttga gccattgctc gctcccttcc ggcgacttgt
     1381 tggcaaaaag gttctttttg ggagccgtgc gcggacggcc cggctgggaa cggcggtccc
     1441 ggtaaacggg cctgggacgg gaatcacggg aattacggaa atcgttcatg aaatgctgga
     1501 gagaaaggtt ggatctgcgg catagcgggc cacggccatg tcgatggcct cttcatacgc
     1561 cctcacgggc tgcttcatca gggaagagag ccgttcgcag gacatggcgg tatgccgggg
     1621 ccgagcatcc ctgaaaccgg cctgttcgtc gagcttttgt tccgccacag gcggcaaagc
     1681 aggcagcaac ccgtgcttca cggcgcattt cagtacggtt acggcataac tgtgccagga
     1741 aacaggctct ccggactggc acaaatgcag cacgccggaa gccccgctct cataagccag
     1801 aaaacggagc cagacgcaca aatcttccac ccatgtgggc atggaccatt tgtccgccac
     1861 ggcggccagg ggctcccttt tcatcgcccg ccgcagcacc atttccggga atgaaggccg
     1921 tcccggattg ccgaataccc aggaaacgcg ggccaccaga gcatccggca tctcctcccg
     1981 cacacggaat tccgcctcca gcttggactc cccgtacaca ttcacggggc ggcacttccc
     2041 ctcctccgtt ttcaggcctt ctctcctgcc gtccagcaca tagtccgtac tcaaatggat
     2101 aaagcgcata ccctccaggc ggcaaatgcg cgccatcatc tccggagcca tggcgttgac
     2161 gcgatgggcc gtctccggat catccaggca ggcttccaat ccgcttacag cggcgcaatt
     2221 caccacatgt gtggctccgg aagaaagcag aacacgtttc aaagcgctta aatccttcaa
     2281 atcacaaaag ccccgtccgc acttcaccac ctccaggcca tgttcttcca gatacgctgc
     2341 cagacggctt cccacccggc cgtccgctcc gaacaccaat gttctgtaca tgacgcagag
     2401 cttagacgtt tcaccctcca aaatccaatg aaaacctttt ttcatcgtga caaaagatgg
     2461 aaaggttgtt tgaattaaag ttgtgtttta ccatgaatat tctttaacag cctgcggccc
     2521 tctgaacgcc agagccgcgg aagtgtgcag gaagggagcc cttattaaaa cggatgaagg
     2581 cgggtacggc tgcatccagc cgtggccgga actgggagac gccaccctgc aggaggaatt
     2641 ggacgcgctc aggaagggcc gtcctctgcc tttgggcatc cgcgccgcgg aatgcgcccg
     2701 aacggacgga gaagcacgcg cgaagggcgt cagcctgttt gacggtcttc acattccgga
     2761 aagccacgcc acgctgccct cctgcgtcag ccccgccacc attcgcatca tggaatccaa
     2821 gggattcaag gcaggaaaaa tcaaggccag cgccaatctg gccgcctccc tggaacggct
     2881 gacgatgctg gcatccatgg tcccctcctg gcgctggcgg ctggatttca acggatgcct
     2941 gaatgaaaac gatgccctga aattctggaa atccctgccc catcatctga aaaccaggat
     3001 agatttcata gaagacccgt gccccttctc catccagagc tgggaacgtc tggtggacgc
     3061 cggcatgcct ctggccctgg acatgggttc ggacgtggaa caccagccgg ctatttcctc
     3121 agacctgccc atcatccgca tcgtcaaacc cgcgcgggaa gcaacgccgg aataccttta
     3181 cgagcctccc gtcttcacta ccgtgatgga tcaccccgta gggcagctct gggccgttta
     3241 ccaggcggcg gagtattacc gcaacttcct tcccacggaa attcccctct gcggcctctg
     3301 cactcatctc ctgtttgagc cggacccctt tattgaccag atgggcggca tgaaccctca
     3361 ggcagctgtt cccggaggca cggggctcgg ctttgacgaa ctattggaaa atattccctg
     3421 gaaaatactg taacaataca gaattacccg gctttcattg aaactacaat gaagcggttc
     3481 ttcccgtgct cccgtgccgg aaccgcccat ggtttccctc agcccgccgg aacttgccgc
     3541 tctgatgacg cgccgccggg gagggcttaa tttcgtattc gcgtcctttg catgcaatca
     3601 gaagccgcgc ttttgccgaa agcgcggctt cttttgtaat gatgatggcg gacaccgcaa
     3661 ccggatcatt ccaactccgg atgctcatca cgcaattttc tattccgggc gtgtggaaaa
     3721 tttaaggccg ggatattctt tcacattgca tccgggcgtc ttgggattca ccagcaccgg
     3781 aacaaaagtg aacatccacc ggcgcgcgga attgttgctg gggctcttca ccagcacgcg
     3841 gttccacccc ttcttcaacg gaaccatgga aggctcccgg aaatggtagt tttcatccac
     3901 cagcacgccg ctgttgcgcg gcttcttcca ttgcggaggg gcaatctccc ggccgttcac
     3961 gaaaaatttg ggatccgcat ggaaccattt gccgggaacg ctcgccgggc cattgcgcca
     4021 gtcggacgtg gcccaggtat gcccgctgat ccagaaaggc accgtctgcg ccttggggga
     4081 ataaatccag gtctgcgcat aataagtatg gttcttgtgg ggataagctc ctatcttcgc
     4141 gccattgaac agcgtcggga aatcgcagta atgcttgaaa atgatggtgg ctccc
//
LOCUS       DAOMSU010000186         4170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2038_length_4170_cov_2.327339, whole genome shotgun sequence.
ACCESSION   DAOMSU010000186 DAOMSU010000000
VERSION     DAOMSU010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2038_length_4170_cov_2.327339"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1345
                     /locus_tag="PK077_07945"
     CDS             224..1345
                     /locus_tag="PK077_07945"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRN24270.1"
                     /translation="MNLPSSRIPWLDNIRVFACILVIASHIVGQFFVSPPFTPDNETF
                     AWSSFYTVLVRVSIPLFFMISGALLLPVRDTTGQFLKKRFTRVLFPFLLWSAFYTVFP
                     WSYETLTGDSFHSVFPLSRVTPDLETMGTNLLLIPLKFSVGIHLWYLYVLMGLYLFLP
                     VISPWVEKAGKAAFAYFLLLWALTLLFPYLQTIFPSYLGKCPWNEYGALHSFSGYLGY
                     MVLGCFLRKYPGNSSFARTACWAVPCLAAAFWFTLHFYRTSTAQAWSSSGDYIGFASI
                     NVALMSVALFVLFQSLTAFRAGSAPRRFLADFSSMSFGIYLVHFVLVGAVYRLFGMLH
                     LLFLPASLSIPLLTAATCLAAWGIIKLLSFLPGSRYLIG"
     gene            complement(1429..2661)
                     /gene="icd"
                     /locus_tag="PK077_07950"
     CDS             complement(1429..2661)
                     /gene="icd"
                     /locus_tag="PK077_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HRN24271.1"
                     /translation="MANLTFTPPADGAAVTMQNGRLCVIPFIIGDGTGPEIWAAASRV
                     IDAAVAKAYQGRRSIAWYEVFAGQKSFDNLGTWLPNETVEAFRTYLIGIKGPLTTPVG
                     GGIRSLNVTLRQDLDLFVCLRPVRYFNGIETPVKAPEKVDMVVFRENTEDIYAGIEFK
                     EGTEDAKLFFDTMSRIFPDRMKKVRFPESSGFGIKPVSREGTERLVRAAIDYAVKNGR
                     KSVTLVHKGNIMKFTEGGFRDWGYDVARREYGALPIGDGPWLKLPNGIVIKDCIADAF
                     LQEILLHPENFDVVATLNLNGDYISDALAAQVGGIGIAPGGNINYLTGHSIFEATHGT
                     GPKLAGLNKANPSSVILSAEMMLRYMGWTEAADLLIQAIDRVISEKTVTFDLAEMIPG
                     STEITCSAYGDKLVETLS"
     gene            complement(2788..3480)
                     /locus_tag="PK077_07955"
     CDS             complement(2788..3480)
                     /locus_tag="PK077_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HRN24272.1"
                     /translation="MSGSSPTFFHSPAIVALARGAVLIGVLAVLFWGVRTCITAPAEK
                     PMAVAEKLIEAGREAGLTLINKGFSSNHGGVALIVQKDEKVANLVTVERTFEYEYRYS
                     TTWWWSRKTLILKAAYRAHGGINLEGPEPLRIIIPAGNKGPITAEGLHGKLISCEMVE
                     GSLRIVKDDAGIWNRLTSEDAAIAVNQLNENARKHIMESGLKHQAEENFLKRLREEAG
                     QPEEPSGKGRWF"
     gene            complement(3576..3680)
                     /locus_tag="PK077_07960"
                     /pseudo
     CDS             complement(3576..3680)
                     /locus_tag="PK077_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029308938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
BASE COUNT          805 a         1164 c         1147 g         1054 t
ORIGIN      
        1 ttgtcccggt aaaattgcag ggactttttc atgtctatga ctccaaggca gatgccggtt
       61 actctgttca tgttcttctt catggaattg gcgaatagcc tgtttttctg tttaatgttt
      121 tgcggagcct ccgccaggaa aatgtgtttt tcccggtttt gttaacgtct ggttttatgg
      181 tgaaagaaac ggagccgtcc gcaggtagtt aaggaacctt ttcatgaatc ttccttcctc
      241 ccgcattccc tggctggaca acatccgtgt ttttgcctgt attctcgtta ttgccagcca
      301 catcgtgggg cagttttttg tgtcgcctcc tttcactccg gacaatgaaa cctttgcctg
      361 gtcctccttt tatacggtgc tggtgcgggt ctccatcccc ctgtttttca tgatttccgg
      421 cgccctcctg ctgcccgttc gggatacgac ggggcaattc ctgaaaaaac gcttcacccg
      481 cgtcctgttc cccttcctgc tgtggtctgc gttttacact gtttttccgt ggagctatga
      541 aacgctcacg ggggacagct ttcactccgt cttccccctc tcacgggtca cgccggacct
      601 ggaaaccatg ggaacgaacc tgctgctgat tccactgaaa ttcagcgtag gcattcatct
      661 ctggtacctc tacgtgctga tgggccttta cctcttcctg ccagtcatat ccccctgggt
      721 ggagaaagcg ggaaaggcgg cctttgccta cttcctgcta ctgtgggcgc tcaccctctt
      781 gttcccttat ctccagacca ttttccccag ctatctggga aaatgcccct ggaatgaata
      841 cggggccctg cattccttct ccgggtacct gggctacatg gtgctcggct gctttctgag
      901 gaaataccct gggaattcct ccttcgcccg cacggcctgc tgggccgttc cgtgtctggc
      961 cgcggccttt tggtttaccc tccatttcta ccggacgtcc acggctcagg cctggtcctc
     1021 aagcggcgac tacatcggct tcgccagcat taatgtggcc ctcatgtctg tggcattatt
     1081 cgttctcttc cagtcactta ccgcattcag agccgggtcc gcgccgcggc ggttcctggc
     1141 tgatttctcc agcatgagtt tcggcatcta cctggtccac tttgtgctgg tgggagccgt
     1201 ttaccgcctc tttggcatgc tccatctgtt gttccttccg gcttctcttt ccattccgct
     1261 gctgactgcg gcgacgtgcc tggcagcctg gggaatcatc aagctgctct ccttcctccc
     1321 cggcagcagg tacctgatcg gataaattcc ggcatggaga caaaaaactg ccctccgcac
     1381 cgtgtggcgg gagggcagtc cggatggaaa aagcgggaaa aacagggatc agctcagggt
     1441 ttccaccagt ttgtcgccgt aagcggagca ggtaatttcc gtggaaccgg gaatcatttc
     1501 cgcaaggtcg aacgtaaccg ttttttcgga aatcacccgg tcaatggcct gaatcagcag
     1561 atcggcggct tccgtccagc ccatgtagcg caacatcatc tccgcagaca ggatgacgga
     1621 gctggggttg gccttgttca gtccggccag tttcggcccg gtgccgtgcg tagcttcaaa
     1681 aatggaatgc cccgtcaggt agttgatgtt gcctccgggg gcgattccga tgccgccgac
     1741 ctgcgccgcc agggcgtcgg agatgtaatc cccgttcaga ttcagcgtgg ctaccacgtc
     1801 gaagttttcc ggatggagca gaatttcctg aaggaaggcg tctgcaatgc aatccttgat
     1861 aacgataccg ttgggaagtt tcagccaggg gccgtcccct atggggaggg cgccgtattc
     1921 ccgccgtgcg acatcgtatc cccagtcacg gaagccgcct tccgtgaatt tcatgatgtt
     1981 gcccttatgc accagcgtga cgcttttcct tccgtttttc acggcatagt caatggcggc
     2041 gcgcaccagg cgctccgtgc cttcacggga aacgggcttg atgccgaagc cggagctttc
     2101 cgggaaacga acctttttca tgcggtccgg gaaaatgcgg ctcatggtat caaaaaacag
     2161 cttggcatcc tccgtgcctt ccttgaattc gattccggcg tagatgtctt ccgtgttttc
     2221 gcggaaaacg accatgtcca ctttttccgg agctttgacg ggagtttcaa tgccgttgaa
     2281 atagcgcacg ggacgcaggc agacaaacag atccagatcc tgccgcagcg tgacgttcag
     2341 gctgcggatg cctccgccca ccggagtggt gagggggcct ttgatgccga tgaggtacgt
     2401 gcggaaagct tccaccgttt cattcggaag ccaggtgccc agattatcaa aagatttctg
     2461 cccggcaaaa acttcatacc atgcgatgga acgcctgccc tgataagcct tggctacggc
     2521 tgcgtcaatg acacgggagg ccgccgccca tatctcagga ccggttccat caccgatgat
     2581 gaaaggaatg acgcaaagcc tgccgttctg catggtgacg gctgcaccgt ccgcgggagg
     2641 ggtgaatgtt aaattggcca tgacgtgcgt tcctttatag gacgcagccc ggtttggcaa
     2701 gataaaagag cggaatgggg aactgtgctt ctggagcttt gccggctcct gaagcaaccg
     2761 gccttgaaaa tgttttcctc ttgtacctca gaaccagcgg cccttcccgg aaggttcttc
     2821 cggctgcccg gcttcttccc ggaggcgttt gaggaaattt tcttcagcct gatgtttcaa
     2881 accgctctcc atgatatgct tccgggcgtt ttcgttcagc tggttgacgg caatggcggc
     2941 atcttcggaa gtcagcctgt tccagatgcc ggcgtcatcc ttcacgattc gcagggaccc
     3001 ctccaccatc tcacaggaaa tcagcttgcc atgaaggccc tctgccgtaa tggggccttt
     3061 gtttcctgcg ggaatgataa tccgaagagg ttcgggccct tccagattga tgcccccgtg
     3121 ggcgcggtag gccgctttca gaatcagcgt tttcctgctc caccaccagg tggtggagta
     3181 acggtattca tattcaaaag tccgttccac tgttaccagg ttggccactt tttcatcctt
     3241 ctgcacaatc agggccacac cgccgtggtt ggaggaaaaa cccttattga tcagggtgag
     3301 gcccgcttcc ctgccggctt caatcagttt ttccgctaca gccatcggct tttccgccgg
     3361 ggcggtgatg caggtgcgca cgccccagaa caatacggcc agcacaccta tcagaacggc
     3421 acccctggcc agcgcgacaa tggcggggga atggaaaaag gtgggggagg atccgctcat
     3481 gagatgttgt gcgtggcgga aattcagggt tccgggtctt catgggagcc atgttccctg
     3541 atagggggca tgaacagggc caggaaggtg aaggcgatga cggtcaggtc atctatctgt
     3601 cctactcccg gaatgaagtc cggaatcagg tcaatggggg aaaaggcata aagagccgtc
     3661 agagccagga ggacaagctt gcgtctggtg aacaggcgcg gttctccttc ccggctgcgg
     3721 aagtaattga tggccctgac cagaagctgc tgcctggagc cgttgggcgg aatgggaatc
     3781 ttgtttttca tggagatgca ggggtgaatt taccgtcaat ggaaaggaaa ctctttttta
     3841 tatggaccgg aagaagaccc gaaggcagga taacggaccc cttcgtcctg ccttgtcccg
     3901 gcaacggcac gggaagggat ttttttgaag ccgttttctg taggcgtctt ttcatcagga
     3961 aagctctttc cttattgtct catgcactcc cggccgcgtc cagagcaatt cccggcttct
     4021 tccgggaatg agcttccttg catttgagag agagtataac gtttacggca gttgtgccgt
     4081 gcatgtatct ccttttcctg gcattatcat cggcaaggga agagggcaag tgccggaaag
     4141 cgcggaagga accgtaatat ttgttttgtc
//
LOCUS       DAOMSU010000187         4141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2058_length_4141_cov_2.451297, whole genome shotgun sequence.
ACCESSION   DAOMSU010000187 DAOMSU010000000
VERSION     DAOMSU010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4141
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2058_length_4141_cov_2.451297"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="PK077_07965"
     CDS             <1..220
                     /locus_tag="PK077_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24273.1"
                     /translation="FMDALATRLAEKLAMPLTGNQSLRQNLNQEFHKIILPQAATVNA
                     VQCFSNDSHPLLDLLRKIKSPSCPEECE"
     gene            271..564
                     /locus_tag="PK077_07970"
     CDS             271..564
                     /locus_tag="PK077_07970"
                     /inference="COORDINATES: protein motif:HMM:NF015898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HRN24274.1"
                     /translation="MKALDFIQIFASNVRRLDFRLSSAQVILAVIAGYRRHSTITEAT
                     RLHPNTVTNILQDLIAQGYVNRIGDSRPYVYRPTADGEQLAGNLLDKNTFPGT"
     gene            561..818
                     /locus_tag="PK077_07975"
     CDS             561..818
                     /locus_tag="PK077_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24275.1"
                     /translation="MNNPLLSTEEKRRWLARVFRDEDGEYSQADKFKALVEDTKLAAL
                     QQEEEEFKRQREIGAAPQDPILALLQAIPPAELNFNNHSSS"
     repeat_region   1321..1813
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=1522..1541
                     /rpt_unit_seq="gtttcaatccacgcactcgt"
     gene            complement(2210..2875)
                     /locus_tag="PK077_07980"
     CDS             complement(2210..2875)
                     /locus_tag="PK077_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24276.1"
                     /translation="MSAWLTQARRKDVKIFLGIWIFSMTLPYIQMLAPALGYEGNYGN
                     MGILGVCDWNPYGMFYNFSGFLGYMVLAHYLTKYPLAWSWKKTLSITIPLFLIGFAVT
                     FFGFLETQKHFPGQYSKLEVLWYFSGINVFLMTFAIFTIVSRLRIKAGPVLSKVAALT
                     FGVYLCHFFFVQCSYDFVNFIGLGGLPSAVKIPLMACLASAVSAALVWLLSLNRWTRK
                     SIM"
     gene            complement(2872..3363)
                     /locus_tag="PK077_07985"
     CDS             complement(2872..3363)
                     /locus_tag="PK077_07985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRN24277.1"
                     /translation="MNTNQSLNSRGSHIVWVDFLRILACFLVVLAHCCDPFVGSFDGS
                     FNFKSAVFWGSLVRPCVPLFAMISGVLLFPVTLEMGAFYSRRLKRVLVPLIVWSLALP
                     LLYFGYFAAGVQTASPNIVMDTYTWSATVGKLYTFFFNFNYDTTPLWYVYMLVGLYLF
                     MPS"
     gene            complement(3826..>4141)
                     /locus_tag="PK077_07990"
     CDS             complement(3826..>4141)
                     /locus_tag="PK077_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24278.1"
                     /translation="IGTSRPWIQKMHGYYASLTVETEAELIGRMPEALPGIRFLRGTD
                     ISETLANAARARDVEVLDRPVLANGRLELLGYFREQAVSETVHRYGNLIPPPGSFKTD
                     SV"
BASE COUNT         1121 a         1123 c         1027 g          870 t
ORIGIN      
        1 cttcatggac gcactggcca cccggctggc agaaaaactg gccatgcccc tgacgggcaa
       61 ccaaagcctg cgccagaatc ttaaccagga attccataaa atcattcttc cgcaggcggc
      121 taccgtcaat gcggtgcagt gcttttccaa tgattcccac ccgctgctgg acctgctgag
      181 aaaaatcaaa tcgccctcct gcccggaaga atgtgaataa catgagaata ataagcaaat
      241 aacataataa taaattgtga atacatgctc atgaaagcac tggatttcat acagatattt
      301 gcctccaatg tccgcaggct ggacttccgc ctcagcagtg cccaggtcat cctggccgtc
      361 attgccgggt acaggcgcca cagcaccatt acggaagcta cacgcctgca ccccaatacc
      421 gtcaccaata tcctgcagga tctcattgcg cagggatatg tcaaccgtat tggagacagc
      481 cgcccttatg tttaccggcc cactgcggat ggagaacagc ttgccggaaa cctgctggac
      541 aaaaatacat tccccggcac atgaacaatc ccctgctcag tacggaagaa aaacgccgct
      601 ggctggcccg cgttttccgg gacgaggacg gagaatactc tcaggcagac aaattcaagg
      661 cgctggtgga agacaccaaa ctggcggccc ttcagcagga agaggaggaa ttcaaacgcc
      721 agcgggaaat aggcgccgca ccgcaggacc ccatcctggc tctgctccag gccattcctc
      781 ccgcggaact caatttcaat aatcactcct cttcctgaca acttaacaag atgatggaac
      841 aaaaaagctc tctagaccaa acaagctcgg agagcttttt ctttttcaat ccacgcactc
      901 cttgcggagt gcgacaaacg ggaggggaaa taacatggac gcgttgctgg tttcaatcca
      961 cgcactcctt gcggagtgcg actactcgga cggggtgagg cagttgaggt gtgacaggga
     1021 tcatggaaaa ggggtgaatt tggggtgaag aggggtgaaa gcttgatgaa ttaaaggctc
     1081 gcagggtgga caacgtgcag gaagaaagaa ggtgaaaaat tccgcgatag aaatgaattt
     1141 gacagaaaga tggtgaattt ttttcgttga gtgcaatgtc gttgcaagaa gagtgcaaac
     1201 taagagaaat agcggccatg tctgaatcaa atgcacctct tgttataacg ccgatcccag
     1261 tgtacggatc agttactgtc atatctccgc aggaccaggg taatgcccac catgcaccgg
     1321 gtttcaatcc acgcactcct tgcggagtgc gacaactcat tatgctctca atctaaccaa
     1381 tctgcaagtt tcaatccacg cactccttgc ggagtgcgac taaaacacca tgctcaagtt
     1441 tccggcacca tcctcgtttc aatccacgca ctccttgcgg agtgcgacag cgcagcaggc
     1501 catccgcgaa accctgcgcc agtttcaatc cacgcactcg tgagagtgcg acctggaact
     1561 cctgaataac tcctgcctga cgcaactgtt tcaatccacg cactcgtgag agtgcgaccc
     1621 aagggtacag aggattccta gaggttgtag aggtttcaat ccacgcactc gtgagagtgc
     1681 gacgccttta tctctgaccc gctttgcctt gcccttgttt caatccacgc actcgtgaga
     1741 gtgcgacgaa gatgtctggg aactatgtca ttttacaaaa gtttcaatcc acgcactcgt
     1801 gagagtgcga ctcctgaacg tctatggttt gattatcaac agtgcaacgg gtgttttgcg
     1861 ccaacctccc atgggaggta caaagattcc aggccgtgaa aaagagatga tagttgtcaa
     1921 gatcagaacc ccaacggatt acgaaaaccg ccggtgtccc gggaattttc tgtgagcttg
     1981 gggtcggcgg aagggagacc gggaaaggag cttttcatgc gttaaaaagc ccgcatctcc
     2041 ggcaacctag gcctgtacac gatgctggga catcctagcc tgttccctct ttcccgtcca
     2101 gaattttgta ggcctctccc cggaaaccgt tttattctct tttccacaaa agaagagaca
     2161 tcccggtact gccgtgtccc cgaagcgacc tgcgaaaaat atctgcggtt tacatgatgc
     2221 ttttgcgcgt ccacctgttc aggctcaaca gccataccaa cgccgcggag acagcggagg
     2281 ccagacaggc catcaacgga atttttacgg cggagggcag ccctcccagc ccgatgaagt
     2341 tcacgaagtc ataggagcac tggacaaaga agaagtggca cagatacacg ccgaaagtaa
     2401 gcgccgccac cttgctcagc actggaccag ccttgattct gagccggctg acaatggtaa
     2461 agatggcaaa ggtcatcaga aatacattga ttccggagaa ataccagagc acttccagct
     2521 tggaatattg accggggaag tgcttctgcg tttccagaaa gccgaagaac gtaacggcaa
     2581 aaccaatcaa aaagagggga atagtaatgg acagcgtttt tttccaactc caggccagcg
     2641 ggtatttggt caggtaatgc gccagcacca tgtatcccag gaatccggaa aagttataaa
     2701 acataccgta cggattccaa tcgcaaacac ccagaatacc catattgccg taattgccct
     2761 cataccccag tgccggagca agcatctgga tgtaggggag agtcatgctg aatatccaga
     2821 tgcccaggaa gattttcaca tccttcctcc tggcttgcgt cagccacgcg ctcatgaggg
     2881 catgaagagg tacaggccca ccagcatgta tacataccac aggggcgtgg tatcataatt
     2941 gaaattgaag aagaaggtat acagcttgcc gacggtggcg ctccaggtat aagtgtccat
     3001 cacgatgttg gggctggccg tttgaacgcc tgcggcaaag tatccgaagt agagcaaggg
     3061 aagcgccagt gaccagacaa tgagcggaac cagcaccctc ttgaggcgcc tggagtaaaa
     3121 agcgcccatt tccaaggtga cgggaaagag caacactccg gagatcatgg caaacaaagg
     3181 cacgcacggc cgtaccagac ttccccagaa gacagcggat ttaaagttga aggaaccgtc
     3241 aaaacttcca acgaacggat cacagcaatg ggccaggaca acgaggaagc aggccaggat
     3301 acgcagaaaa tccacccaga cgatgtggct gccgcgggaa ttcaatgatt ggttggtgtt
     3361 catggttacg tggaaaaaac agcgccgcgt catcgcaaac gttcctgata atatacccgt
     3421 ttcccggaaa cgttctatca ttaaatggag aaaagaaaca aaaagcaccc aatccctccc
     3481 ccggcaacag gggaaagctc ttcgggaaag cctgcgcttc ctgcaggatt ccacgcatct
     3541 tcccccataa gaatcatgaa accttttccc gccccaccgg aattgttcac cgtgaggcct
     3601 ggtgaaaatt ttctcattcg gcgaagttca tatttcattc aacaggatac tcctcttccc
     3661 tccttgcaaa gaaaaatttt tcccctcctt tcagtgaaga gctattccga aacggaaggg
     3721 gaacacaccc aacacccatg ttttcctttt gaaccggcag gaggatttcc cttcattttt
     3781 atggctaaga tacccggaag ccgggattca ggccttccct acccttcaca cgctgtctgt
     3841 tttaaagctg cccggcggcg ggatgagatt gccgtagcgg tgaacggttt cggaaacagc
     3901 ctgttcccgg aaataaccca gcagctccaa ccgcccattg gcaagcacgg gacggtccag
     3961 tacttccaca tcccgggcac gggcggcatt ggccagagtc tcggaaatat ctgttccacg
     4021 caggaaccgt atgcctggaa gggcttccgg catccgtccg atcaattccg cttccgtttc
     4081 caccgtcagg gaagcataat aaccatgcat tttctgaatc caggggcggc tcgtccctat
     4141 g
//
LOCUS       DAOMSU010000188         4138 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2059_length_4138_cov_2.652951, whole genome shotgun sequence.
ACCESSION   DAOMSU010000188 DAOMSU010000000
VERSION     DAOMSU010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4138)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4138
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2059_length_4138_cov_2.652951"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="PK077_07995"
     CDS             <1..764
                     /locus_tag="PK077_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HRN24279.1"
                     /translation="VTPDMMQGADLSGISGLHFHTLCEQGADDLAVTMDAVERHFGHL
                     LSRPEISWLNMGGGHWITKPDYDRKLLAALIRRIRERYGVEVWLEPGEAAAIHTGVLR
                     CRVLDLFSAEGVNHAILDVSASAHMPDTLEMPYRPDVFQIAGDASLRSAQQPAVRLEG
                     ESYALAGKAGEKAYTYRLGAPTCLAGDRLGDYSFAEPLAVGDELVFDDMAHYTMVKTT
                     FFNGVRHPDIAVQRKDGSVETVRRFSYEDFEARLG"
     gene            788..2725
                     /gene="speA"
                     /locus_tag="PK077_08000"
     CDS             788..2725
                     /gene="speA"
                     /locus_tag="PK077_08000"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HRN24280.1"
                     /translation="MATAKEGIKQKVRGWSHHDSADLYGIDDWGNGYFRISKKGEVTV
                     RLNDEDGGKKDVSLCDLLEGLKERGTTVPVLFRFRDLLRSRIAELNDSFRKAIKEAGY
                     QGKYQGVYPIKVNQQRQVVEEIAEFGTKYDYGLEAGSKPELIAAMAHMHNPNAYLICN
                     GYKDDEFIDLALTAQKMGLNIFIVLEMPSELDVIMERARRMDIRPNLGVRVKLAAKGS
                     GLWQESAGDKSVFGLNAAQVVDVVDKLKQVDALDCLKLLHYHQGSQIPNISVVREGLT
                     EAVRIYVDLVKEGAPLGTLDMGGGLAVDYDGSKTNFHSSCNYSIAEFARDVVEVVGDM
                     CSKYGVPHPTLVTESGRAVVAYYSVLVFNVLDVTSAPGEEPPPELPDDAPELLKAFAD
                     TDRTLARKNMQECYNDACYYRDQLRAQFFYGNATLRQRGLGEAYYWHILSRISRMLAE
                     METIPEDLRELSCSMVDFYYGNFSLFQSLPDSWAIRQLFPVMPLHRLNERPTNKAVLA
                     DITCDCDGKIDHFIDREDVATALPLHAIKPGEDDYYIGVFLVGAYQETLGDLHNLLGD
                     TNVVGVHLEGGRPVYTHEVEGDTVSDVLSYVEYDPKELINKFRTFAEQAVAEGKISPS
                     ERRRALEVFRSGLNGYTYYEL"
     gene            complement(2796..3890)
                     /locus_tag="PK077_08005"
     CDS             complement(2796..3890)
                     /locus_tag="PK077_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRN24281.1"
                     /translation="MKKAPNTNKLKYVGAALLEGESVTLIQAARLVLEIKEALGDEIC
                     TITRCREVVSLGLNAIKNKHQTVSFGTAAVECLRSKSHRRPRTLTDIRSIIHKLKKSN
                     PELEHTSLRNLSVEECQNILMNTFTTSRQRHKARLILSGIFSFSVKRGWCDENPILRV
                     DTPFLQEQEIPALTLKEITQLLKAAMEEFDGSCAAGAALMIFAGIRPQEVERLLWENI
                     ALRDGCIILNSKHTKTGGARHVTILPVLAKWLKFCRDRTKPGPGTPICPKGWTIKWRK
                     IRKKAGWGGRKKSWVPDCLRHTYASYHAKHFKDYNLLQMEMGHRSSSLLRTRYLNMKG
                     ISPQTATRFWALTPAKVIEETKPPEEPLVS"
BASE COUNT          826 a         1016 c         1281 g         1015 t
ORIGIN      
        1 gcgtgacgcc ggatatgatg cagggggcgg acctgtccgg catttccggt cttcattttc
       61 atactttatg cgaacaggga gcggatgacc tggcggtaac gatggatgcg gtggaacgcc
      121 acttcggcca tcttctgagc cgcccggaga tttcttggct gaatatggga ggaggtcatt
      181 ggattaccaa acccgactat gaccggaagc tgctggcggc acttatccgc cgcatccgcg
      241 aacgctacgg cgtggaagta tggctggaac cgggagaggc cgctgccatt cataccgggg
      301 tgctgcggtg ccgggtgttg gacctttttt ccgcggaggg ggtgaatcat gccattctgg
      361 atgtttccgc atccgcccac atgccggata cgctggaaat gccttatcgg ccggatgtgt
      421 tccagattgc aggagacgct tccctccgtt ccgcgcagca gcccgccgta cggttggaag
      481 gggagagcta tgctctggcg gggaaggccg gggaaaaagc ttatacgtac cggctgggcg
      541 cccccacgtg cctggcggga gaccggctcg gagattattc cttcgcggag cccctggcgg
      601 taggtgacga acttgtgttt gacgatatgg cgcattatac gatggtgaag actacgtttt
      661 tcaatggcgt gcgccatccg gacatcgccg ttcagagaaa agacggttcc gtggagacgg
      721 tgcgccgttt ttcctatgaa gattttgagg cccgcctggg ctagaatatt tgaccagaca
      781 gattgacatg gcaacagcta aagaaggcat caagcagaaa gtccgcgggt ggagtcacca
      841 tgattccgca gatttgtacg gcattgacga ttgggggaac ggttatttcc gcatcagcaa
      901 aaagggagag gttaccgtac gcctgaatga cgaggatgga ggaaagaagg atgtcagcct
      961 gtgcgatctg ctggaggggc tgaaggagcg cgggactacc gttcccgttc tgttccggtt
     1021 ccgggatttg ctgcgcagcc gtattgcgga gctgaacgac agtttccgga aagcaatcaa
     1081 ggaggcgggg taccagggta agtaccaggg cgtttacccc atcaaggtga accagcagcg
     1141 ccaggtggtg gaggagattg cggagttcgg caccaagtat gattacggtc tggaggccgg
     1201 ttccaagccg gagctgatag ccgccatggc ccatatgcac aatccgaatg cctatctgat
     1261 ttgcaacggg tacaaggatg acgagtttat tgacctggcg ttgacggccc agaagatggg
     1321 gctgaatatt tttattgtgc tggaaatgcc ttccgagctg gacgttatta tggagcgtgc
     1381 cagaaggatg gatatccgcc ctaatctggg cgtgcgcgtc aagctggcgg ccaaggggtc
     1441 cggtttatgg caggaatccg cgggggataa gtccgttttc gggttgaacg cagcccaggt
     1501 ggtggatgtg gtggataagc tgaagcaggt ggatgcgctg gattgcctga agctgctcca
     1561 ttatcatcag gggtcccaga ttccaaatat ttccgtggtg cgcgaggggc tgacggaggc
     1621 ggtccgcatt tacgtggacc tggtgaagga gggcgcccct ctgggcacgc tggacatggg
     1681 cggcggcctg gccgtggatt atgacggttc caagacgaat ttccattctt cctgcaatta
     1741 ttccattgcg gagtttgccc gtgacgtggt ggaggttgtg ggagacatgt gttccaagta
     1801 cggggttccc catcccacgc tggtgacgga gtccggacgg gccgtagtgg cctattattc
     1861 cgtgctggtg ttcaacgtgc tggatgtgac gagcgcgccg ggcgaggaac ctccgcccga
     1921 gcttccggat gacgctccgg agctgttgaa ggcttttgcg gatacggacc ggacgctggc
     1981 caggaagaat atgcaggagt gctataatga cgcctgctat taccgggacc agttgagagc
     2041 ccagtttttt tacgggaacg ccaccctacg gcagcgcggg ctgggagagg cttattattg
     2101 gcatatcctg agccgcattt cccgcatgct ggcggagatg gagaccattc cggaggatct
     2161 cagggagctt tcctgctcca tggtggactt ttattacggc aatttttcac tgttccagtc
     2221 tcttccggat tcctgggcca tccgccagct tttccccgtg atgcctctgc accgtttgaa
     2281 tgaacggccc acgaataagg ccgtgctggc agacatcacg tgcgattgcg acggaaagat
     2341 agaccatttc attgaccgtg aggacgtggc aaccgccctc cccctgcatg ccatcaaacc
     2401 cggcgaggac gattattaca taggcgtatt cctggtagga gcctatcagg agaccttggg
     2461 ggatttgcat aacctgctgg gagatacgaa tgtggttggc gtgcatctgg aaggcggccg
     2521 ccccgtttat acccatgaag tggagggcga tacggtgtcc gatgtccttt cctacgtgga
     2581 gtatgatccc aaggagttga tcaacaagtt ccgcacgttt gcggagcaag ccgtagccga
     2641 ggggaaaata tctccgtccg aacgccggag agccttggaa gtattccgtt ccggattgaa
     2701 tggatacact tattacgaac tgtaggaaac ggtcttgtct tttgcttcaa cagggagcgt
     2761 ttgtcccgtg ccggcgcgtt ttcctttgtt gcggatcagg agaccagcgg ttcttccggc
     2821 ggtttcgttt cttcaatgac cttggctggc gtcagggccc agaagcgcgt ggccgtttgc
     2881 ggagagatgc ctttcatgtt caagtaccgt gtgcggagca gggaggagga gcggtgcccc
     2941 atttccattt gcaacaggtt gtagtccttg aagtgcttgg cgtggtagct ggcgtaggtg
     3001 tgccgcaggc agtccggcac ccatgatttt tttcttcctc cccagccggc ttttttccgg
     3061 attttgcgcc acttgattgt ccaccctttc gggcagatgg gagttccggg tccgggtttg
     3121 gtcctgtcac ggcagaattt gagccatttg gcgagcacgg gcaggatggt gacgtgcctg
     3181 gcgcctccgg ttttggtatg cttggagttc agaatgatac agccgtcgcg gagagcgatg
     3241 ttttcccaga gcaggcgttc cacttcctgc gggcggattc ctgcaaagat catcagcgcc
     3301 gctccggctg cgcagcttcc gtcaaattct tccatggctg ccttgagaag ctgagttatt
     3361 tccttcagcg tcagagcggg gatttcctgc tcctgcagaa aaggcgtgtc tacgcggagg
     3421 atgggattct cgtcacacca tccgcgtttg acggagaacg agaaaattcc gctcaagatg
     3481 agcctcgctt tgtgcctctg ccgggatgtg gtaaaggtat tcatcaggat gttctggcat
     3541 tcctccacgc tcaggttgcg cagggaggtg tgttccagtt ccggattgct ctttttaagc
     3601 ttgtggatga tgctcctgat gtccgtcagc gtccggggac ggcggtggct tttggaacgt
     3661 aggcattcca cggccgccgt gccgaaactg acggtttgat gtttgttttt aatggcgttc
     3721 aatcccaggg agacgacttc ccggcaccgg gtaatggtac agatttcatc tccaagggct
     3781 tccttgattt ccaataccag ccttgcagcc tggatcaggg tgactgattc cccctccaga
     3841 agtgcggctc cgacatattt caatttgttt gtattcggtg cttttttcat gttcatttct
     3901 cgtgtttgtc caaacacggt tttaatgaaa aaggctatcc ttataatggg gaaaacttat
     3961 gctttccact agaaaacgcg taaccgtcaa ggatatgttg ttacttatgt tttctttgaa
     4021 ccggccgcaa aatcaaagtt tctgatggtg caggaaccga aaatgttaga aagcgcggct
     4081 ccctgtgctg aatgtcaaga tggacgtgac ttgtccggca tgagtatatt tctgtcgg
//
LOCUS       DAOMSU010000189         4130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2072_length_4130_cov_2.661595, whole genome shotgun sequence.
ACCESSION   DAOMSU010000189 DAOMSU010000000
VERSION     DAOMSU010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2072_length_4130_cov_2.661595"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..1676
                     /locus_tag="PK077_08010"
     CDS             60..1676
                     /locus_tag="PK077_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRN24282.1"
                     /translation="MNIATIAALAATLGLPAPAQQSVTKIITENGQPSLALDEQFAAR
                     LRGGGTKQNSPATVPAEFSAAAPGLPLPADANHAAVRETAEERNRRMEWWRDAKFGMF
                     IHYGLYSGLAGEWKGRPGGSEWIQKNVEVDTDTYAAEALPLFKPREGLTEEWAQLARD
                     AGCQYIVLTSKHHEGFGLFDSALTDYDAKSAVNRDIIREYVDSCRRRGLKVGLYHSVI
                     DWHHPSYDNTICPDLCYPAGQAAMLNRKNIPRDHAAYQKYLHAQVRELMTRYAPIDIM
                     WWDYSQGAMEGAKGWKAPELMDMVRSINPGVIMNNRLYAYSGLNKDQAGTLDLRCGDY
                     ITPERFIPRRGYPGVDWESCMTVSDKWGYNRYDTNIKSPETIIEKLVECVTKGGNLLL
                     NVNPMADGTIPEKVAATMRGVGRWLNINGEAVYGTRAFIQLDQPASINRQGDIFVFLI
                     PPPDKGAAQPPSEGTMKELTAGAEHARMQPLDATGYEDDEGTAITLPAGYSKALLLGD
                     NTVLPVVNGTVLFKAHEHSKTPCSVIKLSK"
     gene            1701..4085
                     /gene="ligA"
                     /locus_tag="PK077_08015"
     CDS             1701..4085
                     /gene="ligA"
                     /locus_tag="PK077_08015"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRN24283.1"
                     /translation="MEEDLFSLAAGKQPSQQATNETASRAGEARENAGAGHPGNAEDP
                     AHRRIDYLRAELRRHNRLYYEQAEPEISDAEYDALFLELEKLEEAHPELADPDSPTRR
                     VGGAPLQGFNQIRHAVPMLSIDDIFEQRDAPVPDEELAEFYHKLARALQTEKVPVSVE
                     PKIDGVALSIMYRGGKLAYAATRGDGDVGDDVTANVRTIRSVPLTLPPGAPPVLEVRG
                     EVFMPNEAFAKLNEERDADGLPAFANPRNATAGTLKQLDPRQVAARPLAFLAHGLGAY
                     EGPELRDAKGFWDMLRHCGIPCNEPVYYTDSLESTRQAVRDIDSLRHTLPYGTDGAVI
                     KISSMATREALGATARAPRWAAAYKFPPEQKETTLLNIIVQVGRTGVLTPVAELRPVL
                     LSGSTVARATLHNQDEIDRKDIRIGDTVLVEKAGEIIPAVLKVNLSKRPADARPYSIL
                     ETTRGLCPACGNPIMKEEGKVAWRCTNFTCPAQAVTGITHFCSRSALDVESIGSSVAE
                     ALRSSGLASSALDLFSLTTDQLATLNLGTPEEPRRYGEKNARKALDALQNARGLPLER
                     WLIAFGIPLVGEVVAKALADTHPDLKHVADSPYLRDIVRLDELMEKAAKTNPNTRGNK
                     KAVKEGALSAEAVQERHQELMEEIDRLTAPYLATGYLRKNTAKFSYGSEIGVAAAKSL
                     QSFFASAAGNHTMDVLNGLGINPQSQSYRANFLEIPAGALSGKTFVITGTLSHPRDYF
                     EQLIASHGGKATGAISKSTSFLLAGNGGGSKRDKALKLGVPVISEEDFYKMIGN"
BASE COUNT          929 a         1343 c         1189 g          669 t
ORIGIN      
        1 cggacgtcat ttaagccggt tcagccttcc cctccagcct cacaccatcc tgaacaacca
       61 tgaacatagc cacgattgcc gctctggccg ccactctggg gcttcccgcc ccggcccagc
      121 agtccgtcac taaaatcatt acggaaaacg ggcagccctc tctggccctg gacgaacagt
      181 tcgcggcccg gctgcgcggc ggtggaacaa agcagaactc cccggccacc gttcccgcgg
      241 aattttcggc agccgcgccc ggactccccc tccccgcgga tgccaaccat gccgcagtca
      301 gagaaacggc ggaggaacgt aaccggcgca tggaatggtg gagggacgcc aaattcggca
      361 tgttcatcca ctacggcctg tattccggac tggccgggga atggaaaggc aggcccggag
      421 gttccgaatg gattcaaaaa aacgtggaag tggatacgga cacctacgcc gcggaagccc
      481 tgcccctgtt caagcccagg gaaggactga cggaagaatg ggcccagctc gcccgggatg
      541 cggggtgtca atatatagtg ctcaccagca agcaccatga aggcttcggc ctgtttgaca
      601 gcgccttgac ggattacgac gccaaaagcg ccgtgaacag ggacatcatc cgcgaatatg
      661 tggactcctg ccgcaggcgc ggcctgaaag tgggcctcta ccactccgtc attgactggc
      721 accacccgtc ttatgacaac accatctgcc cggatctctg ctaccctgcg gggcaggcgg
      781 ccatgcttaa caggaaaaac attccccgcg accacgccgc ctaccagaaa tacctgcacg
      841 cccaggtccg ggagctgatg acccgttacg cgcccatcga tatcatgtgg tgggactact
      901 cccagggggc catggagggg gccaaaggct ggaaagcgcc ggaactgatg gacatggtgc
      961 gctccatcaa tcccggcgtc atcatgaaca accgcctgta cgcctattcc ggcctgaaca
     1021 aggaccaggc cggaacgctg gacctgcgct gcggggacta tattactccg gaacgcttca
     1081 tcccccggcg cggatatccc ggcgtggact gggaatcctg catgacggtg agcgacaaat
     1141 ggggctacaa ccgctacgac accaacatca agtccccgga aaccatcatt gaaaaactgg
     1201 tggaatgcgt taccaaggga ggcaatcttc tgttgaacgt caatcccatg gcagacggca
     1261 ccattccgga aaaagtggcc gccaccatgc gcggcgtagg ccggtggctc aacatcaacg
     1321 gtgaagccgt gtacggcaca cgggcattca ttcaactgga ccagccggcc tccatcaatc
     1381 ggcaggggga tatcttcgtc ttcctgattc cgccgccgga caaaggagcg gcccagccgc
     1441 ccagcgaagg aaccatgaaa gaactgacgg cgggcgcgga acacgcccgc atgcagccgc
     1501 tggacgccac cggttatgaa gatgatgagg gaacggccat caccctgccc gccggctatt
     1561 ccaaagcatt gcttttgggg gacaacaccg tccttcccgt cgtcaacgga acggttctgt
     1621 tcaaggctca tgaacattcc aaaacaccct gttccgtcat caaactgagc aaatagccat
     1681 tgttcccact catttttcct atggaggaag accttttttc cctggcagcc ggcaaacagc
     1741 cttcacagca ggcaacgaat gaaacggcat cccgggctgg ggaggcgcgg gaaaacgccg
     1801 gcgccggcca ccccggaaac gcggaggacc ccgctcaccg ccgcatagac tacctgcgtg
     1861 ccgaactgcg ccgccacaac cgtctgtatt acgaacaggc ggaaccggag atttccgacg
     1921 cggaatatga cgccctgttc ctggagctgg aaaaactgga agaagcccac cccgaactgg
     1981 cggatccgga ctcccccacg cgccgcgtag gcggcgcccc cctccagggc ttcaaccaga
     2041 tcaggcatgc ggtgcccatg ttgtccattg acgacatttt tgagcaacgg gacgcccccg
     2101 tgccggatga agaactggcg gaattctacc ataagctggc cagggcgctg caaacggaga
     2161 aagttcccgt ctccgtggaa cccaagattg acggagtggc cctttccatc atgtaccgcg
     2221 gcgggaagct ggcttacgcg gccacgcgcg gagacggaga cgttggggac gacgtaacgg
     2281 ccaacgtacg caccatccgc agcgtccccc tcactctccc tcccggcgcg ccgcccgtgc
     2341 tggaagtgcg cggggaagtc ttcatgccca atgaagcctt cgccaaactg aacgaagaac
     2401 gggacgccga cgggctcccc gccttcgcca acccgcgcaa cgccaccgcc ggaaccctca
     2461 agcagctgga cccccggcag gtggccgccc gtccgctggc cttcctggcc catggactgg
     2521 gcgcgtatga aggcccggaa ctcagggacg caaaaggctt ctgggacatg ctccgccatt
     2581 gcggcatccc ctgcaatgaa ccggtgtatt acacggacag cctggaatcc acccgccagg
     2641 ccgtgcggga tattgacagc ctccgccata cgctccccta cggcacggac ggagccgtta
     2701 ttaaaatcag ttccatggct acgcgggaag cgctgggcgc tacggcgcgc gcgccccgct
     2761 gggcggcggc gtataaattc cccccggaac agaaggaaac caccctgctg aatattatcg
     2821 tgcaggtagg acgcaccggg gtgctgacgc ccgtggcgga attgcggcct gtgcttctgt
     2881 ccggttccac ggtggcgcgg gccaccctgc ataaccagga tgaaatcgac cggaaggaca
     2941 tccgcatcgg agatacggtg ctggtggaaa aggcggggga aatcatcccg gccgttctga
     3001 aagtgaacct ctccaaacgc ccggcggacg ccaggcccta cagcatactg gaaaccacgc
     3061 gggggctgtg ccccgcctgc ggcaatccca tcatgaagga agaagggaaa gtagcctggc
     3121 gctgcaccaa cttcacctgc cccgcgcagg ccgtgacggg catcacccac ttctgttccc
     3181 gttccgctct ggatgtggag agcatcggct cttccgtggc ggaagcgctg agaagctccg
     3241 gtctggcatc ttctgcgctg gaccttttct ccctgactac ggaccagctc gccaccctga
     3301 acctgggcac gccggaggaa ccgcgccgct atggggaaaa aaatgcccgg aaagcgctgg
     3361 acgccctgca aaacgcgcgc gggcttccgc tggaacgctg gctcatcgcc ttcggcattc
     3421 ccctggtggg ggaagttgtc gccaaggcgc tggcggacac gcatcccgac ctgaagcatg
     3481 tggcggattc tccctacctc cgggacatcg tccgcctgga tgaactgatg gaaaaggccg
     3541 ccaaaaccaa ccccaacacc cgcggcaaca aaaaggcggt gaaggaaggc gcgctttctg
     3601 cagaagcggt acaggaacgc caccaggaac tgatggagga aatcgaccgc ctgaccgcgc
     3661 cctatctggc aacgggctac ctgcgtaaaa acacggccaa attcagctat ggctccgaaa
     3721 taggcgtggc ggcggccaaa tccctccaaa gctttttcgc ctccgcagca gggaatcaca
     3781 ccatggacgt cctgaacgga ttgggcatca atccccagtc ccagtcctac cgggccaact
     3841 ttctggaaat cccggcgggg gccctgtccg gcaagacctt cgtcattacg ggaacgctca
     3901 gccatccgcg ggactacttt gaacagctta tcgcctccca cggaggaaaa gctaccgggg
     3961 ccatctccaa atccacctcc ttcctcctgg caggcaacgg aggaggctcc aagcgggaca
     4021 aagccctcaa gctgggcgtg cccgtcattt ccgaggaaga cttctacaaa atgattggaa
     4081 attaattgcc gtaaaacagc gggaacggaa agcaggaact gaaacgcgcg
//
LOCUS       DAOMSU010000190         4108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2091_length_4108_cov_2.456699, whole genome shotgun sequence.
ACCESSION   DAOMSU010000190 DAOMSU010000000
VERSION     DAOMSU010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4108
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2091_length_4108_cov_2.456699"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..83)
                     /locus_tag="PK077_08020"
     tRNA            complement(2..83)
                     /locus_tag="PK077_08020"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(47..49),aa:Leu,seq:tag)
     gene            complement(365..745)
                     /locus_tag="PK077_08025"
     CDS             complement(365..745)
                     /locus_tag="PK077_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24284.1"
                     /translation="MYIPTQEERYAKKGVVSLILCVVVPSCLYVSLLSKQLMMVRGHE
                     WEAVMKLFLAFTYPLAALFAMTGIPFFWWSVGISLAVHAGIVAWLKTTPLLSPKKAVC
                     IALCLGMLDFLFVKLFPFPAQIMG"
     gene            832..2979
                     /locus_tag="PK077_08030"
     CDS             832..2979
                     /locus_tag="PK077_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRN24285.1"
                     /translation="MSSFELNSSFKPSGDQEQAIGKLLKSLEAGNKHQGLLGVTGSGK
                     TFTMANLIARMNRPTLVISHNKTLAAQLHSELKSFFPNNAVDYYVSYFDYYQPEAYIA
                     SSDTYIEKDSAINDELDRLSLNAMNSLMTRRDVIVVASVSCIYGLSTPEDYRNLTLRM
                     REGDVMDRDVFLKQLVERLFERQDFDFTRGTFRVRGEVVEVFPAYSEGEAIRVEFFGD
                     EVERVSLIDSATGRVRERLGSYTFFPAKQYVAAPEKRAAALKAIREELEDRVGWFEKH
                     GRLLEAQRLKLRTDYDLELIEELGFCKGIENYSRHLSGRLPGSAPSTLLDFFPKDSLT
                     LIDESHVAVPQLGGMYEGDRSRKNILVEHGFRLPSALDNRPLKFHEFMERQNQIVYAS
                     ATPGPFELVNCRADNKTYIPVRRAARSGEKAPEGFKGILFTSPKDIRVAPSPSTEPVE
                     KFDPTRRSTPLIVEQIIRPTGLLEPKITIRPLKGQIDETIAMCNERVAKNERVLVTTL
                     TKKTAEDLTEYLKGVGLKVSYIHADVDAIERVEILRALRARKIDVLVGINLLREGLDL
                     PEVSLVCILDADKEGFLRNETSLVQTAGRAARHLNGECVLFADVMTDSIKRLVELTDY
                     RRGIQEKYNREHGIVPQTVSRSEQGQLKLYAEDDEESFRVAEDEAGYGLEERIARLEA
                     EMKEAASHLEFERAALIRDEIRQMRGEYGKGRG"
     assembly_gap    3265..3274
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3538..3747
                     /gene="rpmI"
                     /locus_tag="PK077_08035"
     CDS             3538..3747
                     /gene="rpmI"
                     /locus_tag="PK077_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009960242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRN24286.1"
                     /translation="MTRKGGINKTRKAVAKRFKVTGTGKVLRRKQGKRHILQKKSSKR
                     KRQLGKVALVDETQVWAVKQNLPFA"
     gene            3897..>4108
                     /gene="rplT"
                     /locus_tag="PK077_08040"
     CDS             3897..>4108
                     /gene="rplT"
                     /locus_tag="PK077_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRN24287.1"
                     /translation="MPRATNGPASRKRRKRILLRAKGFRGFRSKLFRYAKDAVYKAWQ
                     YEYRDRKRQKGQFRRLWIARISAAVR"
BASE COUNT          976 a          963 c         1212 g          947 t
ORIGIN      
        1 gtgcgcctgg caggactcga acctgtacgc ctttcggcac cagatcctaa gtctggcgcg
       61 tctaccaatt tcgccacagg cgcaacgaat gggcggacgc actctgccat accaggctct
      121 ggcgttcaag attaaacagg agtttttacc tttgacgcgc ttgtgggaag cgcctgtggt
      181 gagaacccat aggaaagtta gctgtcctgt tgggtgtctg ctttctggcg gcattttcca
      241 ttcatgggcg gatgtctggt tccccaaagg cacatttact ccggattaga aggggggcat
      301 tgaatacgtc ttggtttgtc tgccggtaaa gaatggaata tcccagatat tttcccgggc
      361 aggcttatcc catgatttgt gccggaaaag gaaatagttt cacgaacaga aagtccagca
      421 tccccaggca cagggcgatg cagacggctt ttttagggga caggagaggc gttgttttca
      481 gccaggcgac gatgccggca tgaaccgcca gagagatgcc cacagaccac cagaagaacg
      541 gaatgcccgt catggcgaag agggccgcca gcgggtaggt gaatgccaga aagagtttca
      601 tgacagcttc ccattcatgc ccgcgtacca tcatcagctg cttggacaaa agagagacat
      661 acaggcagga agggactaca acgcacagga tgagggaaac aacccctttt tttgcatacc
      721 gttcttcctg tgtgggaatg tacatgccgc atatactggg tcaggagggg aaaattgtca
      781 aacattgtcc tagcccggcg gggcgggagc aatatactga cggcattgcg tatgtcgtct
      841 tttgagttga attcctcctt caagccttcc ggagaccagg agcaggccat cggcaagctg
      901 ctcaagtctc tggaggcggg taacaagcac caggggctcc tgggagtgac cgggagcggc
      961 aagacattca ccatggcgaa tttgattgcc cggatgaaca ggcccacgct ggtgatttcc
     1021 cacaacaaga cgcttgccgc acagcttcat tcggagctga agagtttttt cccgaataac
     1081 gcggtggatt actacgtttc ctatttcgat tattaccaac cggaggcgta tatcgccagt
     1141 tccgacacct acattgaaaa ggattccgcc attaatgacg agttggaccg cctgagcctg
     1201 aatgccatga attccctgat gacacggagg gatgtgattg tggtggcatc cgtttcctgc
     1261 atttacggcc tgtccactcc ggaggattac aggaacctga cgctgcgcat gcgcgagggg
     1321 gatgtcatgg accgggacgt gtttttgaag caactggtgg aacgcctgtt cgagcggcag
     1381 gattttgatt ttacccgcgg cacgttccgc gtgcgcggtg aggtggtgga ggtgtttccc
     1441 gcttattcgg aaggtgaagc aatccgcgta gagtttttcg gcgacgaggt ggaacgggtt
     1501 tctttgattg actccgccac cgggcgtgtg agggaacggc ttgggagcta cacttttttc
     1561 ccggccaagc agtatgtggc cgctccggag aaaagggcgg cggcgttaaa ggctatccgg
     1621 gaagagctgg aggaccgcgt aggctggttt gagaagcatg ggcgcctgct ggaagcgcag
     1681 cggcttaaat tgcgcacgga ttatgatttg gaactgattg aagagctggg tttctgcaag
     1741 gggattgaaa attattcccg ccatttgtcc ggccgcctgc ccggaagcgc cccatccacc
     1801 ctgctggatt tctttcccaa ggattcattg accctgattg atgaaagtca tgtggcggtg
     1861 ccgcagctgg gaggcatgta cgagggggac cgaagccgca aaaatattct ggtggagcat
     1921 gggttccgcc tgcccagcgc tctggataac aggcctttga aattccatga gtttatggaa
     1981 aggcagaacc agattgtgta tgcttccgct actccggggc catttgaact ggtaaattgc
     2041 cgtgcggata ataagacgta tattcccgtg cgccgtgccg ccagatccgg agaaaaggct
     2101 ccggaagggt tcaaaggaat tttgtttacc tctcccaagg acattcgcgt tgctcccagc
     2161 ccatccacgg aaccggtaga aaaatttgac ccaaccaggc gttctacacc cctgattgta
     2221 gagcagatta tccggcctac cgggctgctg gagccgaaga ttaccatccg tccattgaaa
     2281 gggcagattg acgagacgat cgccatgtgc aatgaacgcg ttgccaaaaa tgaacgcgtg
     2341 ctggtcacga cgctgaccaa gaagacggcg gaagatttga cggagtacct gaaaggggta
     2401 ggattgaagg tgtcctatat tcacgcggat gtggatgcga ttgagcgtgt ggagatttta
     2461 cgtgctttga gagccaggaa gatagacgtt ctggtaggca ttaacctgct tagggaaggt
     2521 ttggatttgc cggaggtttc cctggtgtgc attctggatg cggacaagga ggggtttttg
     2581 cgcaatgaga catccctggt gcagacggcg ggccgcgccg cgcggcattt gaatggagag
     2641 tgcgtcttgt ttgcggatgt gatgacggat tccatcaaac ggctggttga gctgacggat
     2701 taccgccggg gtattcagga aaaatacaac agggaacacg gtattgtccc ccagacggtg
     2761 agccgttccg aacaggggca gttgaagttg tacgcagagg atgatgaaga gtccttccgc
     2821 gtagcggagg atgaagccgg gtatggtttg gaagagcgca ttgccaggct ggaggcggaa
     2881 atgaaggagg ctgcttccca tttggaattt gaacgcgccg ccttgattcg ggatgaaatc
     2941 aggcagatgc gtggagaata tggaaaaggc aggggataat atccggcgtg atgaattggg
     3001 gatgagcttt ctggtaggat ggcatgctgt tttttcgtga tatttgtcca tcttattctt
     3061 ggccgtcatt tgacggtgat ttggatattg ggtttaacag ttaattgctt aaagaattct
     3121 gctgatgtga cgtcagtgtt ccggaccgga gtccgctgtc aacgtgaaat attctaagaa
     3181 ggccttacac cgatggggaa gtatatgaag ggctctttgt tccagtcttt tcttatatat
     3241 aagaaaagac tggaacaaag agccnnnnnn nnnntatata taagaatttc tcatgataag
     3301 cacttgccga agtggcaaag ggtatggcgg atgacagagg attcccaggc tggcagcagt
     3361 agctgtctga ttatcctttg ccggttatgt tccccatttt atttcttgat gcagccggct
     3421 gcgtcaaagg cgccgggcaa tgtatggtca ggaagggccc atttgccatc gtctgaagaa
     3481 aagatttgca taaaaaatac ttgcgtattt ggaaaaagcc tgtaatctcc ttccaccatg
     3541 acccgcaaag gtggtatcaa taaaactcgc aaggcagtcg ccaagcgttt caaggttacc
     3601 ggcacgggca aggtactgcg ccgtaaacag ggcaagcgcc acatcctcca gaagaagtcc
     3661 agcaagcgca agcgccagct tggcaaggtg gctctggtgg acgagacgca agtctgggca
     3721 gtcaagcaga acctgccttt cgcctaaacc gcggaaacgc tcgactaatg ccgagctttc
     3781 cccccgctga acggggggcc ttcatgcagc cttcttccga tgagaagata agaacaggcg
     3841 agactgcagg gaggtgagga agaacagtct gttcaagata gaaatagaaa taagctatgc
     3901 cacgtgccac taatggaccg gcctctcgca agcgtcgtaa gcgcattctc ttgcgtgcca
     3961 agggtttccg cggtttccgc agcaaactct tccgctacgc caaggatgct gtttacaagg
     4021 cttggcagta tgaataccgc gaccgcaagc gtcagaaggg acaattccgc cgtctttgga
     4081 tcgcccgtat ttccgctgcc gtccgcga
//
LOCUS       DAOMSU010000191         4057 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2129_length_4057_cov_2.758371, whole genome shotgun sequence.
ACCESSION   DAOMSU010000191 DAOMSU010000000
VERSION     DAOMSU010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4057)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4057
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2129_length_4057_cov_2.758371"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..789
                     /locus_tag="PK077_08045"
     CDS             193..789
                     /locus_tag="PK077_08045"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRN24288.1"
                     /translation="MPRLSIHLPDGSEKTIVLPKNGEYFARIGRDEHCEIVLPFQSVS
                     GEHALLQFKEGGYVLEDLGSTNGTKINGLTPMGAATLYDGDEIALGDARLRFAEEPSP
                     GFSAELSGREEEAAPSIAMRNLQQLADEAARTTRNHYLWMALYAVLIFFLAVFAGLTY
                     KHYRVTGELLPLQWLGIESPKAALKSMPLPQTENGMQL"
     gene            complement(814..1362)
                     /locus_tag="PK077_08050"
     CDS             complement(814..1362)
                     /locus_tag="PK077_08050"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRN24289.1"
                     /translation="MKTTFPPHGRALLHQTPAGAVVIVENGTAVTHLFFSRMAQPEHL
                     EWEETPLLRQTADQLDEYFQGSRRVFDVPLSPQGTEFERTVWKALQAIPYGETRSYGD
                     IARRIGRPSACRAVGQANNRNPVGIIIPCHRVIGADGKLTGYASGLTIKQYLLELEQG
                     SATPFTLFTDVERMPGNTPASP"
     gene            1722..2420
                     /locus_tag="PK077_08055"
     CDS             1722..2420
                     /locus_tag="PK077_08055"
                     /inference="COORDINATES: protein motif:HMM:NF012925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HRN24290.1"
                     /translation="MRLEDGCLVLETHFEKFANVNYKKSSADWIKNTKFMPYTSGSVT
                     TIKTKNFMFGRLEVRAKVPKTKGIWPAIWLLGKNKWGWPVNGEIDMLENISQQPDVVY
                     STFHLSPDGVSKRDASRGGTVKIENLSDDFHTYVMEWDKDSIKLMVDDKLVKSIDLNT
                     TNYANGAGNPFRTPFYLILNSAVGGTWCEKAPKDGQGYPVKFLVDYVRFYQTKEHAQQ
                     AKQFDPETGLPKKK"
     gene            complement(2710..3576)
                     /gene="ilvE"
                     /locus_tag="PK077_08060"
     CDS             complement(2710..3576)
                     /gene="ilvE"
                     /locus_tag="PK077_08060"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HRN24291.1"
                     /translation="MKIWLDGKLVEQEEAKTSVFDHGTLYGDGIFEGIRFYNKRVFRL
                     EDHMDRLYNCAHYLLLDIPYTQEELSNAVCETVAASGLNDGYIRLVVTRGVGNLGLNP
                     FNCKRSCVFIIADKISLYDPDVYENGLALITSSVRRNRPDTVCPQVKSLNYLNNILAK
                     MEAVRQGAAEALMLNDLGNVAECTGDNIFIVKDGTVFTPPVTDGCLDGITRRVVLEIC
                     RELQIPAQEKTMNRFTITCADECFLTGTAAECVPVTKLDGYQLGSGKIGPVTARILAR
                     FQELAQTTGTSC"
     gene            3784..>4057
                     /locus_tag="PK077_08065"
     CDS             3784..>4057
                     /locus_tag="PK077_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRN24292.1"
                     /translation="MSDPSKFRNLAIIAHVDHGKTTLVDQLLQAGGAYRANQAVQERA
                     MDSMDLEREKGITIKAKNTSILWNGYTINIVDTPGHADFGGEVERVM"
BASE COUNT          952 a          965 c         1124 g         1016 t
ORIGIN      
        1 gcaattgctt aaaatatgtt tcgaaagtct tcgggaaaaa taaaaattga ggaggatgaa
       61 cccatatcat gaatctgcat ggaattctgt ctttctgggg aaaggcggat atcgccattt
      121 ttcttatacg tgatgggcaa acggtctttt ggattgaaaa tgaatatggg cgtggcatga
      181 ttttcaggaa tcatgccacg cctttccatc caccttcccg acggttctga aaaaaccata
      241 gttcttccaa agaacggtga atatttcgca cgcatcggac gtgacgagca ttgcgagatc
      301 gtgcttccct ttcagtcggt ttccggagag catgccttgc ttcagtttaa ggaaggggga
      361 tatgtcctgg aggaccttgg ttctactaat ggaactaaaa tcaacggcct gacgccgatg
      421 ggcgccgcaa ccctttacga tggcgatgaa atcgctttgg gggatgccag gctccggttt
      481 gcggaagagc catctccggg attttctgcc gaattatccg ggagagaaga agaggccgct
      541 ccttcgatcg ctatgcggaa cttacagcag ttggcggatg aagccgcccg cacgactcgg
      601 aatcattatt tatggatggc tctttatgcc gtcctgatat tttttctggc tgtgtttgcc
      661 ggattaacct acaaacatta cagggtaacg ggtgaattgc tgcctcttca atggctgggt
      721 attgaatccc ccaaggccgc cttgaaatcc atgcctcttc ctcagacgga aaacggtatg
      781 cagctctgac cggaattttc cagtcctgcc gttttatgga ctggccggag tatttccggg
      841 catgcgttcc acatccgtga ataatgtgaa aggagtggcg gagccctgtt ccaattccaa
      901 caggtattgt ttaatggtca ggccgcttgc atagccggtc agtttgccgt cggctccgat
      961 gactcggtgg caagggatga tgatgccgac agggttccga ttgttggctt gtcctactgc
     1021 ccggcatgct gatgggcgtc ctattcgccg ggcaatgtct ccgtaacttc tggtttctcc
     1081 ataggggatg gcttgaaggg ctttccagac cgttcgttca aattcggttc cttggggaga
     1141 aagcggcacg tcaaatacgc ggcgggaacc ttgaaaatat tcatccagct ggtcagccgt
     1201 ttgacgcagc agaggtgttt cctcccattc caaatgctcc ggctgtgcca tgcggctgaa
     1261 gaaaagatgg gtgacggctg tgccgttttc cacaatcacg acagcacctg caggcgtctg
     1321 gtggaggagc gcccgaccat ggggagggaa agtggttttc atatcatgac catagcatgc
     1381 gccgggttaa gggttctatt cattttgcag gatatttcgc catttgacag gggaaaggag
     1441 gtatgtggtc ccgaatgatg tattttgttt gaaaggctgc ggaactaggt gcagtattgc
     1501 tccgtatgaa gagcctattt gccgtgttgt tgactgcatg cctttgcatg tctgtaaagg
     1561 gagaagattc caaacctccg aggaccctcc ccggcgggtg ggtttatgtc tggggtgacg
     1621 agttcaacgg ttccaggata gacgccaaaa agtggaaacc ggagttggga gtaatccgta
     1681 accagggatc ccagcagact tatacaggcc gacccaagaa tatgagattg gaggatggct
     1741 gcctggtttt ggaaacccat tttgagaagt tcgccaatgt caattataag aaaagttccg
     1801 cagattggat caagaatacc aagttcatgc cctatacctc cggttccgtt acgacgatca
     1861 agacaaaaaa tttcatgttc ggcagactgg aagtgcgtgc caaggttccc aaaaccaagg
     1921 gcatctggcc tgccatctgg ttgcttggca agaataaatg gggctggccc gtcaatggag
     1981 agattgatat gctggagaat atttcccaac agccggatgt ggtttattcc actttccatt
     2041 tgagtccgga cggcgtgtcc aaaagggatg cttcccgcgg agggactgtg aagatagaga
     2101 atctttccga tgattttcat acctatgtca tggaatggga caaggattcc atcaagttga
     2161 tggtggatga caagctggtg aagtccattg accttaatac caccaattat gccaatgggg
     2221 cggggaatcc gttccgtacg ccgttctatc tcattctcaa ttccgccgtg ggcggcacct
     2281 ggtgcgagaa agctcctaaa gacgggcaag ggtatcctgt aaaattcctg gttgattacg
     2341 ttcggttcta tcagacgaaa gaacatgccc agcaggctaa gcagtttgac ccggaaaccg
     2401 gcctgcccaa gaagaaatag ccttttttca ggttgaggca tgtccggcgt ggcggccgga
     2461 catgcaatat gtcacttgcc ttgtcatttc acaaatggcg cggcttttca tattggattc
     2521 ttctgcagtg tttcggaacg ggaggccgtg aaggtcacaa gccttcattt ccagaggaga
     2581 ctgccgtata gccctgcggg aatgtgctta cctgtgtttt cttttttata tttaacggaa
     2641 tagcacaaaa aaacggctcc gtcatctggc ggagccgttt tcgttggtcc aaaaaagggt
     2701 tggctggttc taacaggatg ttcccgtggt ctgggccagt tcctggaaac gggccaggat
     2761 tcgagctgta acaggaccga tttttccaga gcccagctga tagccgtcca gtttggtcac
     2821 gggaacgcat tccgcggcag tgccggtcag gaagcattcg tccgcacagg tgatggtaaa
     2881 gcggttcatg gtcttttcct gagccgggat ttgcagctcc cggcagattt ccagcactac
     2941 ccggcgcgtg atgccgtcca ggcagccgtc cgtaactggc ggtgtgaaca cggtaccgtc
     3001 cttcacgatg aaaatgttgt cgcccgtgca ttcggccaca ttacccaggt cgttcagcat
     3061 cagggcttcc gccgcaccct ggcgcacagc ttccattttg gccaggatgt tgttgagata
     3121 attgagcgac ttgacctgcg gacaaacggt gtccggacgg ttgcggcgca cggagctggt
     3181 aatcagggcc agcccgtttt catatacgtc cggatcgtac agggagattt tatccgcaat
     3241 gatgaagacg caggaccgtt tgcagttgaa tgggttgagc cccagattgc cgacgccgcg
     3301 ggtaaccacc agacggatat agccgtcgtt caggccggag gccgcgaccg tttcgcatac
     3361 ggcgttggag agttcttcct gagtgtaagg aatatccagc aacagataat gggcgcagtt
     3421 gtacaggcgg tccatgtggt cttccaagcg gaaaacgcgc ttgttataga accggatacc
     3481 ttcaaaaatg ccgtctccat agagtgtgcc gtggtcgaaa accgaggttt ttgcttcctc
     3541 ctgttccacc agctttccat ctaaccaaat cttcattgtt atctagaatt gagaaattac
     3601 ttatgccgga gcagaatact cctatttgag gaggggggga agctataaat tcaccggagg
     3661 aaaataaaag ttccatcgct tgcggcaatg gcggcaggga ggcttgcaaa cgctgaaaat
     3721 atagtcggta ttcatggagc agacttgaca tttattttcc ggtgttttta gctctcgcgc
     3781 gcaatgtcag atccttccaa gttccgcaac ctcgccatta tcgctcacgt tgaccatggg
     3841 aagacgaccc tggttgacca acttttgcag gcgggaggcg cctaccgggc caaccaggcc
     3901 gtgcaggaac gcgccatgga ttccatggat ctggaacgtg aaaaaggcat cacgatcaag
     3961 gccaagaata catccatcct ctggaatgga tacacgatca acattgtgga tactcccgga
     4021 cacgcggatt tcggcgggga agtggagcgc gtgatga
//
LOCUS       DAOMSU010000192         4056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2132_length_4056_cov_2.532117, whole genome shotgun sequence.
ACCESSION   DAOMSU010000192 DAOMSU010000000
VERSION     DAOMSU010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2132_length_4056_cov_2.532117"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="PK077_08070"
     CDS             <1..252
                     /locus_tag="PK077_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRN24293.1"
                     /translation="PGLGRSTDPSETVDEAPDKIGAKIRNARLDRVPYMLVLGQREAE
                     DGTVSVRHRDKGDLGAMPFEQFADLVAREIAERHISPVI"
     gene            292..930
                     /gene="infC"
                     /locus_tag="PK077_08075"
     CDS             292..930
                     /gene="infC"
                     /locus_tag="PK077_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRN24294.1"
                     /translation="MPTKPTKNNDGNRRRDRGDQTRINERIRAPRVRVVTANGDQLGV
                     MNTRDALEKAKALGLDLVEVAGNADPPVCRVVDYGKYKYQQAKLQKNNKSRTVKLKEI
                     KLRIGTDTNDYNVKMARTESFLDHGHKVRFQLRFRGRENAHQELGYDVFNKVIADMKT
                     MAQVDQAPRLAGNTMHMVLSPLPAQQRVKKFTAHLDKDFDSEDSAFDAEDDE"
     gene            1044..1262
                     /locus_tag="PK077_08080"
     CDS             1044..1262
                     /locus_tag="PK077_08080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN24295.1"
                     /translation="MFLSVQWESGNRLGRLEKVLLHYEAVFSAWDENRLAGLVAVMDD
                     GTMTAYVHYLLVYPRYQGYGVGSWLPGI"
     gene            complement(1388..2620)
                     /locus_tag="PK077_08085"
     CDS             complement(1388..2620)
                     /locus_tag="PK077_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HRN24296.1"
                     /translation="MTARTPAGTPPPAARITILDILRALALLGIVIVHTHDHFNLYLP
                     MPPAEGWQAAANSAADWAYEHLFVSKSFLLFSFLFGLSFFIQLDRREQQGIDFRKRFM
                     WRLTLLFLLGLAHTLFYDGDILTLFGTLGFALVMLYKCGTPLLITLCALCLIQPVMLM
                     DLLNHAGLADWWPHSSGWFHTGSPAAGPTREFLYAHGSWGQAALWNLTQGQAGKWQFF
                     LLSGRLWQTLGLFILGMLAGRWRAFEDAPNKRSLFLRTLGVAGLLFLALLAMRLYLIP
                     QLGAPLEADAARLLHPWENLFYTAAFVSAAVLLFTHPGLPLPTRLLSSAGKCTLTCYV
                     TQTLVFTFLFFGWGLGLAQDMGPWPCLCAAVAVFLLQAWACRLWLSHFLYGPLEWLWR
                     TATMCRMQPFCKRMSKEN"
     gene            complement(2625..3800)
                     /locus_tag="PK077_08090"
     CDS             complement(2625..3800)
                     /locus_tag="PK077_08090"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HRN24297.1"
                     /translation="MQKRRVVILGSTGSIGTSALKVARDIPDRMEIVGLAAGTSVEAL
                     ARQAREFNVRSVCIFDSSAADELACALPGAQVETGEEGLCRLAQLSEADMVLISIVGT
                     AGLKPALAAIEAGKDLAIASKEILVMAGQIVMEKARRAGVQVLPVDSEHNAIFQCLNG
                     NHGGPEAVSRLILTASGGPFRTWNREDLEHVTLEQALKHPTWSMGRKITIDSATLFNK
                     GLEMIEARWLFDVPMEKVDVIVHPQSIVHSMVEYRDGTVLAQMSSSDMCFPIQYAVTW
                     PDRVPNSLKQLNFAEIGRLVFEAPRTDVFPALDLARRAGNGNSTLAAVYNATNEAAVN
                     AFIHGQISFPGIWKLVEAVMDDHTPADPRGELAPILEADQWARRRAAELLPSFSRPC"
BASE COUNT          827 a         1142 c         1332 g          755 t
ORIGIN      
        1 cccggcctcg gacgaagcac cgacccctcg gaaacggtgg acgaagcgcc ggacaaaata
       61 ggcgccaaaa tccgcaatgc ccgtctggac cgcgttccct acatgctggt gctcggccag
      121 cgggaggcgg aagacggcac cgtctccgtc cgccaccggg acaaggggga cctgggcgcg
      181 atgccctttg agcaattcgc ggacctcgta gcccgggaaa tcgcggagcg tcatatttct
      241 cccgtgattt gagttgccaa gttattcctt ttagtttagt agtatccgga cgtgccaacc
      301 aagccaacga agaataatga cggaaatcgc cgccgtgacc gcggcgatca gactcggatc
      361 aatgaacgga ttcgcgcgcc ccgcgtgcgg gtagtgactg ccaacggcga ccagctgggc
      421 gtcatgaaca cgcgcgacgc gctggaaaaa gccaaggctc tggggttgga tctggtggaa
      481 gtggccggca atgccgatcc gcccgtatgc cgcgtggttg attacggcaa gtacaaatac
      541 cagcaggcca aactccagaa aaacaacaag agccgaaccg tcaagcttaa ggaaatcaag
      601 ctccgcatcg gtacggatac caatgactat aatgtgaaaa tggcccgtac ggaaagcttt
      661 ctggatcatg gccacaaggt ccgtttccag ctccgtttcc gcggtcgtga gaacgcccac
      721 caggagcttg gctacgacgt cttcaacaaa gtcatcgcgg acatgaagac catggcgcag
      781 gtggaccagg ccccgcgcct ggccggcaac accatgcaca tggtgctttc cccgctccct
      841 gcccagcagc gcgtgaagaa attcaccgcc cacctggata aggattttga ttccgaagac
      901 tccgcctttg acgcggaaga cgacgagtaa aacggcattt tttctttttc aggctttccg
      961 cccttataag gcggaaagcc ttttttacgg gagaatgctt ttctatagag acccggggaa
     1021 ttctcccgga ggcactggag gaattgtttc tttccgttca atgggaatct ggaaaccgtc
     1081 tgggacgcct ggagaaggta ttgctccatt acgaggctgt gttctccgct tgggatgaga
     1141 acagactggc ggggctggta gccgtcatgg atgacggaac catgacggct tatgtccatt
     1201 acctgctggt gtatcctagg taccagggat acggtgtcgg ttcgtggctg ccgggtattt
     1261 aagcatcgtt ctcacttcct gaagggcagg aatggtttta ttccgggccg gacgcaggag
     1321 gctatgcgtt ctattcctcc tacgttcgga gaaatcggga tggaacggga agaaggtgaa
     1381 taatatctca attttctttg gacatccgtt tgcagaaagg ctgcatgcgg cacatggtgg
     1441 cggtgcgcca gagccattcc agggggccgt acaggaaatg gcttagccag aggcggcagg
     1501 cccatgcctg gaggaggaag acggccacgg ccgcgcacag gcatggccac ggtcccatgt
     1561 cctgggccag gcccagcccc cacccgaaga agaggaaggt gaagaccagc gtctgggtga
     1621 cgtagcaggt cagcgtgcat ttgccggcgc tgctgagcag ccgggtgggc aggggaaggc
     1681 cgggatgggt gaacagcagg acggcggcgg agacgaaagc cgcggtatag aagaggtttt
     1741 cccatgggtg caggagacgg gctgcatccg cttccagagg ggcgcccagc tgcgggatga
     1801 ggtacaggcg cattgccagc aaagccagga acaggagccc ggccacgccc agcgtccgca
     1861 ggaacaggct gcgtttgttg ggcgcgtctt caaaggcgcg ccatctgccc gccagcatgc
     1921 ccaggatgaa gaggcccagc gtctgccaga ggcggccgct gagcaggaag aactgccatt
     1981 tgcccgcctg gccctgcgtc aggttccaca gggccgcctg accccagctg ccgtgggcgt
     2041 acaggaattc ccgcgtggga cccgcagccg gggagcccgt atggaaccag ccggaggagt
     2101 ggggccacca gtccgccagc ccggcatggt tcaacaggtc cataagcatg accggctgaa
     2161 tcaggcacag ggcgcagagg gtgatgagca ggggagtccc gcatttgtag agcatgacca
     2221 gggcaaagcc cagggtgccg aagagggtga ggatgtctcc gtcatagaac agggtatgcg
     2281 ccagacccag caggaacagg agcgtcaggc gccacatgaa gcgtttccgg aagtcgattc
     2341 cctgctgctc ccgcctgtcc agctggatga agaagctgag tccgaacagg aaggagaaga
     2401 gcaggaagga tttgctgaca aagagatgtt cataggccca gtccgccgcg ctgttggccg
     2461 cagcctgcca tccttccgcc gggggcatgg ggagatagag gttgaagtgg tcgtgcgtgt
     2521 ggacgatgac gatgcccagc agggccagag cccggaggat atccagaatg gtgatgcgcg
     2581 cggccggggg aggcgttccg gcgggagtcc gggcggtcat ggcgtcagca ggggcgggaa
     2641 aaggatggga gaagttccgc cgcgcgcctc ctggcccatt ggtccgcttc cagaatgggg
     2701 gccagttccc cgcggggatc cgccggggtg tggtcgtcca tgacggcctc caccagtttc
     2761 cagatgccgg ggaaagagat ttggccgtgg atgaaagcat tcaccgccgc ttcattggtg
     2821 gcgttgtaaa cggctgccag ggtgctgttg ccatttccgg ccctgcgcgc caggtccagg
     2881 gcagggaaga catccgtgcg gggggcctca aacaccagcc ggccgatttc cgcgaaattg
     2941 agctgcttca gggagttggg aacgcggtcc ggccatgtga cggcgtactg gatggggaag
     3001 cacatgtccg agctgctcat ctgggccagc accgtgccgt cccggtattc caccatggag
     3061 tgcacgatgc tctgcgggtg gacgatgacg tccacttttt ccatgggaac gtcaaagagc
     3121 cagcgagcct caatcatttc cagcccttta ttgaacaggg tggcggagtc tatggtgatt
     3181 ttgcggccca tgctccaggt gggatgcttg agggcctgtt ccagcgtaac gtgttccagg
     3241 tcttccctgt tccaggtgcg gaagggtccg ccggaggccg tcagaatcag gcgggagacg
     3301 gcttccgggc cgccgtggtt gccgttcagg cactggaaaa tggcgttgtg ttcgctgtcc
     3361 accggaagca cctgcacacc ggcccggcgg gctttttcca tgacgatttg cccggccatg
     3421 accagaattt ccttgctggc gatggccagg tccttgcctg cttcaatagc ggccagcgcg
     3481 ggtttgagac cggcggttcc cacgatggag attaacacca tgtccgcctc cgagagctgc
     3541 gccaggcggc acagcccttc ttctcccgtt tccacctgcg cgccggggag agcgcacgcc
     3601 agctcgtccg ctgcggacga gtcgaagatg catacgctgc gcacgttgaa ttcgcgcgcc
     3661 tggcgggcca gcgcctccac gctggttcct gcggccagcc ccacgatttc cattctgtcc
     3721 ggaatatccc gggcgacttt cagagcgctg gttccgatgg aaccggtgga gcccaggatg
     3781 acgacgcgtc gtttctgcat aacggagaag catcatgcaa aaaatacggt cccttgacaa
     3841 gccgggggga tgaaaatttt ccccgtgccg catgggccgt tttcccgtca gcccggtttt
     3901 attccggcgg gccggggaaa ggccgccacg cgttcgtgcc gcaggcggtt tgtgccgtgg
     3961 gttgctgcgt tcctgctggc tcgtcaagca gggatggcgg gaaaagagcg ggcctggaac
     4021 gtgaaggaat gttccgccgc atggaaaagg gttgta
//
LOCUS       DAOMSU010000193         4056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2134_length_4056_cov_2.332667, whole genome shotgun sequence.
ACCESSION   DAOMSU010000193 DAOMSU010000000
VERSION     DAOMSU010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2134_length_4056_cov_2.332667"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1007
                     /locus_tag="PK077_08095"
     CDS             <1..1007
                     /locus_tag="PK077_08095"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRN24298.1"
                     /translation="VSLHLFYLGRVPFFLVLAGYFLGRNITWNKAFNRALWLFIPFMF
                     WNILYVFLVLPHDGASFRLENLIGIRDVFLPGMNLFSADDSHAVPPIGPSWFLRDIII
                     LTLLTPLLVRVKILLFPAVLLFFCFFNTAPDHTETISIGTCAFYLLGVALSSRRIDDI
                     HLLMNKKFGIFFWVSIFMPVVLIALYSAGVIPLWKETAIGMLLGVMMIVYAGIWMEKH
                     LPGLSAKIALLAPACFLTFMLHWPIYGFLPPAMKSSLFAFFTPLLVFAAIVLFYFALK
                     RFAPFLLPYLAHVKNTGRRQAQHGACDETIPLIRGLSGMSQSSERDAGNPMPGTRESF
                     "
     gene            complement(928..2514)
                     /locus_tag="PK077_08100"
     CDS             complement(928..2514)
                     /locus_tag="PK077_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24299.1"
                     /translation="MSQLFYLLLFPAFCFPLSARAKAVPPSVPAVSVRMVHPDSQELD
                     RERSPVPEGYTPYTYEFRGRHGKMQRERLFLKNTPIITESEVEQARIEPERRECLHIT
                     LNTQGGKTMKAATSAMNLGRDRLALVVQGKIKSAPVVQAVISRTFEISGLDGKNEASG
                     LAELLNRQAPTEKNTPFIPQDLALYAVHPESRRLVEEGVLRVPGFRLWKLPAHRQEKT
                     GEEEYLFLGNSPIISGDCARHAEPDMEHPGSLDITWNEEAFRKLERASAGLRPGKDRI
                     AVVLRGTILSTPLFQGMPSRTTLIPGLGDDSRLDALCRAINNTLLPLTEQQKRHVREN
                     VALYPIHPRSRELEQHFLPELLQGKSIRLKSGFRSIPYTCPDFPNPVRTYVFIRPESL
                     IGPEDIQEVERDQGGTISCQLLPGAWEKAFAFMKANPAGQVEAAVVFRGNMRLRFEIK
                     RFSLQLWGMPVLDSIQPNSFIYLPEPEEEQRMYRESFHLAIYGFLEPVCPWFFKSSPV
                     FRAWGSRRPFLMIDSSLKGP"
     gene            complement(2684..3172)
                     /locus_tag="PK077_08105"
     CDS             complement(2684..3172)
                     /locus_tag="PK077_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HRN24300.1"
                     /translation="MSENQDELKVQIKAWLKQQNISRNDFAAECFVSPNTVRNWLAKV
                     AIPKDKEALIRLMMEKTEREKKLKEAARANWKPFVVMLSSEDYKLIEEAARRDNMTVE
                     EWAEATLIKDAQKRMKNYYDDSSLPEDVSLAAEVPEEYGAPRRSGPSSSPPSSARRKP
                     RQ"
     gene            3381..3539
                     /locus_tag="PK077_08110"
     CDS             3381..3539
                     /locus_tag="PK077_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24301.1"
                     /translation="MPLSVHFFRDAEADALNMERCQLDKATLIACAVKRLREDLGNVP
                     DSGKKPSA"
     gene            3536..3673
                     /locus_tag="PK077_08115"
     CDS             3536..3673
                     /locus_tag="PK077_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24302.1"
                     /translation="MTQEIYWELLAWLFRSFLAVTAFAFVVAGYQTLRSRPRRGRSRK
                     K"
BASE COUNT          821 a         1067 c         1137 g         1031 t
ORIGIN      
        1 cggtgagcct ccatctcttt tacctgggga gggttccgtt tttcctggtg ctggcagggt
       61 acttcctggg caggaacatc acctggaaca aggcttttaa ccgggcttta tggctgttca
      121 ttccgttcat gttctggaat atattgtacg tgttccttgt cctgcctcat gacggcgctt
      181 ccttccgcct tgagaattta attggtatcc gggacgtctt tcttccgggc atgaaccttt
      241 tctccgcgga tgactcccat gccgttcccc ccatcggccc ttcctggttc ctgagggaca
      301 ttatcattct cacgctgctg acgcctcttc tggtccgcgt caaaatcctg ctgtttcctg
      361 cggtgctcct ctttttctgt ttcttcaaca cggccccgga ccacacggag acgatttcca
      421 ttggaacctg tgctttttac ctgctgggcg ttgcgctcag ttcccggcgg attgacgaca
      481 ttcatctgtt gatgaataaa aaattcggca tttttttctg ggtaagcatt tttatgcccg
      541 ttgtactgat agcgctgtat tccgccggag tgatccccct gtggaaagag acggccattg
      601 gcatgcttct gggtgtcatg atgatcgtgt acgcaggcat ctggatggaa aagcacctgc
      661 ccggcctgtc tgcgaaaata gctttgctgg ctcccgcctg ctttctgacg ttcatgctgc
      721 actggcccat ttacggtttc ctgcctcccg ccatgaaatc cagcctgttc gccttcttca
      781 cgccgttgct ggtgtttgcg gccatcgtcc tgttttattt tgccctgaaa cgttttgccc
      841 cgttccttct gccgtatctg gcccatgtga aaaataccgg ccggcggcaa gcgcagcatg
      901 gcgcgtgcga cgaaacgatt cctttaatca ggggcctttc agggatgagt caatcatcag
      961 aaagggacgc cgggaacccc atgcccggaa cacgggagag cttttaaaga accacgggca
     1021 aacgggttcc aggaagccgt agattgccag gtgaaaggac tcccggtaca tccgttgctc
     1081 ttcttccggc tcaggaaggt aaatgaaaga atttggctgg atggaatcca gcacaggcat
     1141 cccccacagt tgaagggaga atcttttgat ttcaaaccgg aggcgcatgt tcccccggaa
     1201 aacaactgcc gcctccactt ggcccgcagg gtttgccttc atgaaagcaa acgctttttc
     1261 ccatgccccg ggcagaagtt ggcaggagat tgttccaccc tggtcgcgtt ccacctcctg
     1321 aatgtcctcc ggcccaatga gggattccgg gcggatgaag acataggtcc ggacggggtt
     1381 cgggaaatcg gggcaggtat aggggatgga acggaatccg cttttcaggc ggatggattt
     1441 tccctgcaga agttccggaa gaaaatgctg ttccaattcc cggctgcgcg gatggatggg
     1501 ataaagagcc acgttttccc tcacatgccg tttttgctgt tccgtcagcg gcagaagggt
     1561 gttgttgatg gcccggcaca gggcatccag ccggctgtca tcccccagac cgggaataag
     1621 agtagtgcgg gaaggcattc cctggaataa gggggtggac agaatggttc cgcgcagaac
     1681 gacagcaatc ctgtccttgc cggggcgtag tccggccgat gcccgctcca gtttacggaa
     1741 tgcttcttcg ttccaggtga tgtccaggct tcccggatgt tccatatctg gctccgcatg
     1801 cctggcgcag tctccggaaa tgatgggaga attccccagg aacagatatt cttcctctcc
     1861 cgttttttcc tgccggtgcg ccgggagctt ccagagcctg aagcccggta cgcggaggac
     1921 tccttcttcc accagacggc ggctttccgg atggacggcg tacagggcca gatcctgtgg
     1981 aatgaagggt gtgttttttt ctgtgggtgc ctgccggttg agcagttccg ccagtccgga
     2041 ggcttcattt tttccgtcca gtccggagat ttcaaaagta cgggatatga cggcctgcac
     2101 cacgggggcc gacttgattt ttccctgaac gacgagggcc agcctgtcgc ggcccagatt
     2161 catggcggag gttgccgctt tcatggtttt tccgccctgt gtattcaggg tgatgtggag
     2221 gcattctctt ctctccggct caattcttgc ctgttccact tccgattccg tgatgatggg
     2281 ggtatttttc aggaacagcc gttcccgctg catttttcca tggcggccgc ggaattcgta
     2341 tgtataaggc gtgtatcctt ccggaacagg gctcctttcc cggtccagct cctggctgtc
     2401 cggatgcacc atcctgacgg agacggcggg aacagacggc ggaaccgctt tcgcgcgggc
     2461 cgaaagcggg aaacaaaagg cgggaaaaag cagtagataa aataattgac tcacagtcaa
     2521 cggaaataga acttttggag aacggcaagc aagagaaaag ttgattcggc gcacaacaaa
     2581 ggagaggtgt tcccggcgcc tggggaagcc ctccgcggaa cagcacgctg gcggaagccg
     2641 gcaggcaggc gggacatcag cgggatgcca tgccctccag gatttattgc cgcggcttgc
     2701 ggcgggcaga agagggcgga gatgatgaag ggccggaacg tctgggagct ccgtattcct
     2761 ccggtacttc cgccgccagg gatacatctt ccggaaggct gctgtcgtcg taataatttt
     2821 tcatcctctt ctgggcatcc ttgatcaggg tggcttccgc ccattcctcc accgtcatgt
     2881 tgtcccttct ggcggcttct tcgatgagtt tgtaatcctc ggaactcagc atgacgacaa
     2941 agggcttcca attggctctt gccgcctctt tcagtttttt ctccctttcc gttttttcca
     3001 tcatgagccg gatgagagct tccttgtctt tggggatggc caccttggca agccagttgc
     3061 ggaccgtgtt gggagacacg aagcattccg ccgcaaaatc attgcgggaa atgttctgct
     3121 gtttcagcca agccttgatc tgcactttga gttcgtcctg attctcactc atgcaccctt
     3181 tgtaacgaaa atggtgctat ttttcaacct tttttgcata aagtcatttt aatggtatat
     3241 ttttgttgac tgaaataccg tttcaggtgc attgtagggg cgcaaaaggg ctgttaaggc
     3301 cgttattgca ccaaaaaagg atttttatga agacggaaaa taaaaaaaag agtcagaaaa
     3361 aaattcaaat caaggggaaa atgccgttat cggtccattt tttccgggat gcggaagcgg
     3421 acgcgctgaa catggaacgc tgccagctgg ataaagccac cctgattgcc tgtgccgtca
     3481 aacgcctccg ggaagatttg gggaacgttc ccgattccgg taaaaaacct tccgcatgac
     3541 tcaggagatt tactgggaac ttctggcctg gctgttccgt tcctttctgg cggtgactgc
     3601 gttcgccttt gtcgtggccg ggtaccagac cctgcgcagc cgcccgcgca ggggccgcag
     3661 ccgtaaaaaa taggagggca gggggccggt ttaccgtccg tcacggaatg ccgtctcgct
     3721 ttttaaggga aatgtttttg accttatggg gcattgaaga attccggggc tgtgctcctt
     3781 catatttggg cggtccgtca tgaaaagcag gcggaaatct gccggagaaa gtggacggct
     3841 gcctttctcc ggtagaaggg cgttgcagga agaatggaaa aattccggaa aaggtttcca
     3901 cttttccgcc agcgcagatt ctctaaacgg ctgtcatctt cccaggggcc ggccggatgg
     3961 aaatccgtat tgccagaaac ggggaatggc ggtaacattc cgccgctatg agtattgaga
     4021 gtttcgcaaa tcagcatgtg ctggagttgg tggctt
//
LOCUS       DAOMSU010000194         4051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2139_length_4051_cov_3.572823, whole genome shotgun sequence.
ACCESSION   DAOMSU010000194 DAOMSU010000000
VERSION     DAOMSU010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4051
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2139_length_4051_cov_3.572823"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3439
                     /gene="rpoC"
                     /locus_tag="PK077_08120"
     CDS             <1..3439
                     /gene="rpoC"
                     /locus_tag="PK077_08120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRN24303.1"
                     /translation="RPLVPLEGGRFATSDLNDLYRRVINRNNRLKTLLSLKTPEVIIR
                     NEKRMLQEAVDALFDNGRHGRAVTGAGNRPLKSLSDMLKGKGGRFRQNLLGKRVDYSG
                     RSVIVIGPELKLNQCGLPKKMALILFEPFIIHRLKELGYVHTVRSAKKLIDRKTPEVW
                     DILEEVTKGHPVMLNRAPTLHRLSIQAFEPVLIEGSAIRLHPLVCNAYNADFDGDQMA
                     VHVPLSVEAQMEARQLMLAPNNIFSPASGKPIATPTQDIILGAYFLTHTRAAEVQNNQ
                     DNHHHLPLFESIDEVEYAIAARKIGYHDWIRLHNPDYGKKPSEVVYGDVTKKVIITTA
                     GRVRFNEIWPRELGYINRNVGKKQMGDIIWRCYQTVGKERTVQTLDALKNLGFKEATR
                     SGCSIGIVDMVVPSQKKTEIEKAYAELDKVTRQYKNGIITDGERYQKVVDIWTQTTDV
                     IQAALYRKLEHNEGSKMASPLFMMVDSGARGNKAQIKQLSGMRGLMAKPSGEIIERPI
                     TANFREGLSVLEYFISTHGARKGLADTALKTADSGYMTRKLVDVAQDVIVHAEDCGTS
                     NGITVHAIYDGDEEVASLASRIYGRTSCERIVDPVSGEVIVDINDLINEKQAEQLEKI
                     GIERLKIRSVLTCELKKGCCAKCYGLNLATGQEVKIGEAVGIIAAQSIGEPGTQLTMR
                     TFHVGGTATTAFKQPIVKAKNDGRVIYTEDLRTVENADGNFVVLNKNCSVRIENEQGR
                     ELESYQPVIGTILYVPNGGAIKKDETLATWDPYNVPVIAEKGGIVEFKDMIVGITVSK
                     ETDRETGASSLVVMEHKQELHPQVVIRDAKTREVLAHHAIPAGANLTVKDGETISAGT
                     MVAKTPRKVAKTKDITGGLPRVAELFEARKPKDACTIARVEGIVRLSSKNTSRGKKVI
                     TIETPTGELVDHLVPMNKHVIVHEDDHVHLGDQLTEGPVSPEEILDVCGKERLQEHLV
                     NEVQEVYRLQGVEINDKHVEIIVRQMLRKVVITEPGNTEFLWGDQVDKTTFDRINEQT
                     VAQGGQPAAAKPVLLGITKASLETESFISAASFQDTTRVLTEASTLGKTDTLEGFKEN
                     VIMGHLIPAGTGFSRYSKIEVEPAEGAEEIASASEEEEAAELAENMLNDTINFDNER"
     gene            3803..>4051
                     /locus_tag="PK077_08125"
     CDS             3803..>4051
                     /locus_tag="PK077_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24304.1"
                     /translation="MRLHLPITLLAAVLACYTSVSLAVPTSESPAWGANSTFNNNEPA
                     NEYSVTGSQSVNLDVNSGNNNYSTGLYIGAGSSFTINQN"
BASE COUNT         1026 a         1211 c         1036 g          778 t
ORIGIN      
        1 gcgcccgctg gttcccctgg aaggcggtcg tttcgcaacg tctgacctga atgacctgta
       61 ccgccgcgtc atcaaccgca acaaccgcct gaaaaccctc ctgagcctca aaactccgga
      121 agtcatcatc cgtaatgaaa aacgcatgct tcaggaagcc gtggatgccc tgtttgacaa
      181 cggccgccac ggtcgtgccg tcaccggcgc cggcaaccgc cccctcaaat ccctctccga
      241 catgctgaag ggcaagggag gccgtttccg ccagaacctg ctcggcaagc gcgtggacta
      301 ctccggccgc tccgttatcg ttatcggtcc ggaattgaaa ctcaaccagt gcggtcttcc
      361 caagaagatg gcgctcattc tgtttgagcc cttcatcatc caccgtctga aagagctggg
      421 ctacgtgcac acggtgcgct ccgccaagaa gctcattgac cgcaagacgc cggaggtgtg
      481 ggatatcctg gaagaagtga ccaagggcca cccggtcatg ctcaaccgcg cgcccaccct
      541 gcaccgcctc tccatccagg cttttgaacc ggttctgatt gaaggctccg ccatccgtct
      601 gcacccgctc gtctgtaatg cgtacaacgc ggacttcgac ggcgaccaga tggctgtaca
      661 tgtgcctctg tccgtggaag cgcagatgga agcccgccag ctcatgctgg cgcccaataa
      721 tattttctcg cctgcctccg gcaagcccat tgccacaccc acgcaggaca tcattctggg
      781 cgcgtacttc ctgacacaca cccgtgctgc ggaagtacag aacaatcagg ataatcatca
      841 ccatcttccc ctcttcgaat ccatcgacga ggtggaatac gccattgccg cccgcaaaat
      901 cggctaccat gactggatcc gcctgcacaa cccggactac ggtaaaaagc cttccgaagt
      961 ggtgtatggg gatgtcacca agaaggttat catcactact gccggacgcg tgcgtttcaa
     1021 tgaaatctgg ccccgggaac tcggttacat taaccgcaac gtaggcaaga aacagatggg
     1081 cgacatcatc tggcgctgct accagaccgt cggcaaggaa cgtaccgtac agactctgga
     1141 cgccctgaaa aacctgggct tcaaggaagc aacccgttcc ggctgctcca tcggcatcgt
     1201 ggacatggtg gttccctccc agaaaaagac ggaaattgaa aaagcctatg cggaactgga
     1261 caaggtgacc cgccagtata agaacggtat catcacggat ggggaacgct accagaaggt
     1321 ggtggacatc tggacccaga ctacggatgt catccaggcg gctctgtacc gcaagctgga
     1381 acacaacgaa ggctccaaga tggccagccc gctcttcatg atggtggact ccggcgcccg
     1441 aggcaacaag gcgcagatca agcagctctc cggcatgcgc ggtttgatgg cgaaacccag
     1501 cggcgaaatt atcgaacgcc ccatcacggc caacttccgt gaaggccttt ccgtgctgga
     1561 atacttcatc tccacccacg gcgctcgcaa gggtctggcg gataccgccc tgaaaacggc
     1621 ggactccggc tacatgaccc gcaaactcgt ggacgtggcc caggatgtca tcgtccatgc
     1681 ggaagactgc ggcaccagca acggcatcac cgttcacgcc atttatgacg gcgacgaaga
     1741 agtggcgtcc cttgcctccc gtatctacgg ccggacttcc tgcgaacgca tcgttgaccc
     1801 cgtcagcggc gaggttatcg tggacatcaa cgacctcatt aacgaaaagc aggcggaaca
     1861 actggaaaaa atcggcattg aacggctgaa aatccgttcc gtgctcacct gcgaactcaa
     1921 gaaaggctgc tgcgccaagt gctacggcct gaacctggcc accggacagg aagtgaagat
     1981 cggggaagcg gtcggcatta ttgccgccca gtccatcggc gaacccggca cgcagctcac
     2041 catgcgtacg ttccacgtgg gcggaacggc caccacggcg ttcaagcagc ccattgtgaa
     2101 agccaagaac gacggccgcg tcatctacac ggaagatctc cgcacggtgg aaaacgcaga
     2161 cggcaacttc gtcgtcctga ataaaaactg ctccgtccgc atcgaaaacg aacagggccg
     2221 cgaactggaa tcctaccagc ccgtcatcgg caccatcctg tacgtgccca acggcggcgc
     2281 tatcaagaag gatgaaaccc tcgccacttg ggatccgtac aacgtacccg tgattgcaga
     2341 aaagggcggc atcgtcgaat tcaaggacat gatcgtcggc atcaccgttt ccaaggaaac
     2401 ggaccgggaa accggcgcct cctctcttgt cgtgatggaa cacaagcagg aacttcaccc
     2461 gcaggtggtc atccgcgatg ccaagacccg tgaagttctg gctcatcatg ccattcccgc
     2521 aggcgccaac ctcaccgtga aggacgggga aaccatctcc gccggcacga tggtggccaa
     2581 gacgccccgc aaggtagcca agacgaagga catcaccggc ggtctgcccc gcgtggcgga
     2641 actgttcgaa gcccgcaagc ccaaggatgc ctgcaccatt gcacgcgtgg agggcattgt
     2701 gcgcctcagc agcaagaata cctcccgcgg caagaaggtc attaccattg aaacacccac
     2761 gggcgaactg gtggatcatc tggttccgat gaacaagcac gttatcgttc atgaagacga
     2821 ccatgtgcat ctgggcgacc agcttacgga aggccccgtc tctccggaag aaattctgga
     2881 tgtctgcggc aaggaacgcc ttcaggaaca cctcgtcaac gaagttcagg aagtgtaccg
     2941 cctccagggt gtggaaatca acgacaagca tgtggaaatc atcgtgcgcc agatgcttcg
     3001 caaggtagtc atcacggaac ccggcaatac cgaattcctg tggggagacc aggtggacaa
     3061 gactacgttc gaccgcatca atgaacaaac cgtagcccag ggaggccaac cggccgcagc
     3121 caagcccgtt ctgctcggca tcacgaaggc ctccctggaa acggaatcct tcatttccgc
     3181 ggcatccttc caggatacca cgcgcgttct gacggaagca tccaccctcg gcaagaccga
     3241 tacgctggaa ggcttcaagg aaaacgtcat catgggccac ctcattcccg ccggcaccgg
     3301 attctcccgt tacagcaaga ttgaagtgga acccgcagag ggcgcagaag aaatcgcgtc
     3361 ggccagcgaa gaagaggaag cggcggaact tgccgaaaac atgttgaacg ataccatcaa
     3421 cttcgacaac gaacgctaac ctgttctctc cacccacact tcaagggccg ctccccgttt
     3481 ccggggggcg gtctttttta tgtcttctcc gcgttatttc acgaaaagag gaaaagcgga
     3541 ttgaaaattc ggcgatgatt ccaatattgg cccatgcaat ataaaatttg atattttata
     3601 ttcaaaatga aagtcttata gtatacctcc cttgcttcaa ggggggaggt aaatgaaaag
     3661 gtgaaaatcc cattttagaa atttaaaaaa tcataatgca cttacagtaa aagtatgaga
     3721 atccgggaac tggtattttc aacttgacat cgaattttca atccgttata tacataaaaa
     3781 attaacatct aatattttta taatgcgtct ccatctgcct attaccctgc tggcggctgt
     3841 tctggcttgt tacaccagtg tttccctggc cgtcccaaca tctgaatccc ccgcatgggg
     3901 agcgaactcc acctttaata acaatgagcc cgccaatgag tattcagtta ctgggagcca
     3961 atccgtcaac cttgatgtaa attcaggaaa caacaattat tccacaggac tttatatcgg
     4021 agcaggctcc tcgtttacca ttaatcagaa t
//
LOCUS       DAOMSU010000195         4042 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2149_length_4042_cov_2.241284, whole genome shotgun sequence.
ACCESSION   DAOMSU010000195 DAOMSU010000000
VERSION     DAOMSU010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2149_length_4042_cov_2.241284"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            52..429
                     /locus_tag="PK077_08130"
     CDS             52..429
                     /locus_tag="PK077_08130"
                     /inference="COORDINATES: protein motif:HMM:NF016279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaG domain"
                     /protein_id="HRN24305.1"
                     /translation="MVLPLLTSLEVRLTPRSLYLRNEKLERMVRDDGRIPVPYNVSIR
                     NTGDGAVRIIGKKWTVRSHEDGTQVIEGDELFGSRPLLCQGQIFAFNGLQMLRLPARI
                     QLTLLVRDEAGILYHTDPVSLKW"
     gene            complement(562..>4042)
                     /locus_tag="PK077_08135"
     CDS             complement(562..>4042)
                     /locus_tag="PK077_08135"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRN24306.1"
                     /translation="NFLNAFPEEIRLRGEKLQKDGAVTQIFGNHLYIQGRVEDTYGVH
                     RVNLRLQGNRWFGACSTEDEDLAGAAMYAAMLERMYRGQDLPESPNELNDVPLLDILE
                     EKLGRELDDKEADYVSKLEKRYRRFAIEQEIHDHDMVRLNPRWEIVSYDPLELWPVPP
                     TNILEFWNYIAYAFNKKRLPYPEFMESITDLEKVQRKIHEWERSREVGTWYDRIQSVN
                     ERPPQPPRGELFMRLVSTINEGHFEYRHSLKQDWIPVREKQDLEHLENLHERAAVRMD
                     AQTEILLVSLSDYARAEDALTLDLDQEAACALMNKLFHQPALKGYLVNLDENYFKVVD
                     EPLKWICLDDPIEPDCYALQLATSTGINVSHSVRQLPGQQELYQSDETVFPGPPRWLE
                     STEVEPRYSIPKQVIDSLEGVEFLRKIGASLPPSMEDRVVDIDLRGTFDMKIVRGLTS
                     AETEHVLCEVSARDASGYRTEKLGKDGWDVSHQEPMKNKVLLRFIREHLYPIPGLLEE
                     MGFSYDPQVGAFKARVTRQFPEKFAEWAKQLPKELTVNMDDKLKTLLADPVAAAVRFE
                     VVNQEIDWFDLRIVIDVEGVQLSKEQIRALVAARGGYVRMDDGGWMRLEIKLDNDQRD
                     AVTRLGLDPFDLSGETHRMHAMQLADPKAAEVFDPKAWKRIKDRTAEIQIDVKPDVPS
                     QLQATLRPYQVEGFHFLAYLATNGFGGILADDMGLGKTIQSITYVLWLREEFARKNKS
                     KKKVVIPPVLIVCPKSVLDVWAGETEKFAPGVRVLVIRSKDQANLEDIKKNYDMVVVN
                     YAQLRVCGETLNQIKWLTVILDEGQQIKNPDSKAAKAAREIVSYNRLVLSGTPIENRL
                     LDMWSLMAFSMPGVLGSRSYFKKRFDKRKDPQSQNRLAARLKPFLLRRTKSQVAKDLP
                     PRTEEEVYAKMEGIQSQLYKAELRRIQQALLGLDSDESVKKNSFAILQGLMRLRQICC
                     HPGLVDPKYAKEDSAKMTALFYLLDQLREEGHKVLVFSQFVSMLEIIKNRLEAENRPL
                     NYLTGQTKDRRGEIEKFQTTKDPSVFLLSLKAGGAGLNLTSASYVVLYDPWWNPAVES
                     QAIDRTHRIGQKNKVIAYRLLTKDSVEEKIRILQHQKNQLVANVLGDEGFTSSLGIDD
                     LNFILNHGDDEEG"
BASE COUNT          781 a         1060 c         1205 g          996 t
ORIGIN      
        1 ggctctggcc cctgtggaat cataattcct ctctccggca ggcatacatc tatggttctt
       61 ccccttttga ccagtctgga agtgcgtctt actccccgca gcctgtactt gcggaatgaa
      121 aaactggagc ggatggttcg ggatgatggc cgcatccccg ttccttacaa cgtaagcatc
      181 cgcaatacgg gggacggcgc cgtgcgtatc atcggcaaaa agtggaccgt ccgttcccat
      241 gaagacggca cgcaggtgat tgaaggggat gaactgttcg gttcacgtcc cctgttgtgc
      301 cagggacaga tttttgcgtt caacggcctt cagatgcttc ggctgccggc gcgcatccag
      361 ttgactctcc ttgtccggga cgaggcggga atcctttatc atacggatcc cgtcagcctt
      421 aaatggtgaa ataaaaagcc ctgcaatatt catgcagggc ttttttctgg ataagggata
      481 gaggaagaat ctgaggataa aagtctataa gttaataaaa ctgttcctta attcctattc
      541 cctctaattg ttgcttccgg gttaaccttc ttcatcatcc ccgtggttga ggatgaagtt
      601 cagatcgtca atgcccaggc tggaggtaaa cccttcgtca cccagcacgt tggcgacgag
      661 ctggttcttc tgatgctgga gaatgcggat tttttcttcc acggaatcct tcgtcagaag
      721 gcggtaggcg atcaccttgt ttttctggcc gatgcggtgc gtacggtcga tggcctggct
      781 ttctacggcc gggttccacc acgggtcata cagaacgaca taggaggcgg aagtaaggtt
      841 gaggccggca ccgccggctt tcagggaaag caggaagacg gaggggtcct tggtcgtctg
      901 gaatttttca atttctccgc ggcggtcctt ggtctggccg gtaagatagt taagcggcct
      961 gttttccgct tccagcctgt tcttgatgat ttccagcatg gaaacgaact gggagaagac
     1021 gagcactttg tgcccttctt cccggagctg gtccagcagg tagaagaggg ctgtcatttt
     1081 ggcactgtct tccttggcgt atttgggatc caccaggccg gggtggcaac agatctgacg
     1141 caggcgcatc aggccctgca aaatggcgaa actgttcttt ttgacggatt cgtccgaatc
     1201 cagaccgagc agggcctgct gtatgcggcg gagttccgcc ttgtacagct ggctctggat
     1261 gccttccatt ttggcgtaca cttcttcttc cgtcctggga ggcaggtctt ttgccacctg
     1321 ggatttggtg cggcgcagca ggaatggttt caggcgcgcg gccaggcggt tctggctctg
     1381 cgggtccttg cgcttgtcaa agcgcttctt gaagtaggag cgggaaccga ggactccggg
     1441 catggagaag gccatgaggg accacatgtc cagcaggcgg ttttcaatgg gtgtgccgga
     1501 gagaacgaga cggttgtagg aaacgatttc ccgtgccgcc ttggctgcct tggaatccgg
     1561 gtttttgatc tgctggcctt cgtccagaat aaccgtaagc catttgattt ggttcagggt
     1621 ttccccgcag acgcggagct gggcgtagtt gaccacgacc atgtcgtaat tcttcttgat
     1681 gtcttccaga ttggcctggt ctttgctgcg gatgacgagg acgcgcacgc ctggcgcaaa
     1741 tttttccgtt tctcccgccc agacgtccag cacggatttg gggcaaacga taagaaccgg
     1801 agggatgacg acttttttct tggatttgtt tttgcgggcg aattcttcac gcagccagag
     1861 gacgtaagtg atggactgga ttgttttacc caggcccatg tcatccgcca gaatgccgcc
     1921 gaacccgttc gtggcaaggt aagccaggaa atggaaccct tccacctgat acgggcgcag
     1981 ggtggcctgg agctgggagg gaacatccgg cttgacgtcg atctggattt ccgcggtacg
     2041 gtccttgata cgtttccagg ccttcgggtc gaacacttct gcggctttcg ggtccgccag
     2101 ctgcatggcg tgcatgcggt gggtttctcc ggaaagatcg aaggggtcca ggcccaggcg
     2161 ggtaacggcg tcgcgctggt cattgtccag tttaatttcc aggcgcatcc atcctccgtc
     2221 gtccatgcgg acgtaaccgc ctctggcggc gaccagggcg cggatttgct ccttggagag
     2281 ctggacgcct tccacatcaa tgacgatgcg gaggtcaaac cagtcgattt cctggttgac
     2341 tacttcaaac cgcacggcgg cggccaccgg atccgccagc agcgtcttga gcttgtcgtc
     2401 catattgacg gtcagctctt tcggaagctg tttggcccat tccgcgaatt tttcggggaa
     2461 ctggcgggtg acgcgggcct tgaacgcgcc cacctggggg tcgtaggaaa agcccatttc
     2521 ttccagcagg ccgggaatgg gataaaggtg ctcccggatg aagcgcagca gaactttgtt
     2581 cttcatgggc tcctgatggg atacgtccca gccgtccttg ccgagttttt ccgtgcggta
     2641 gccggaagcg tccctggcgc ttacttcgca gagcacgtgt tccgtttccg cagaggtgag
     2701 gccgcggacg attttcatgt caaaggtgcc gcgcaggtca atgtccacca cgcggtcttc
     2761 catgctgggg ggcagggaag cgcctatttt acggaggaat tccacccctt caaggctgtc
     2821 gatgacctgt ttgggaatgg agtagcgggg ttccacttcc gtgctttcca gccagcgggg
     2881 ggggccggga aagacggttt cgtcagactg gtacagttcc tgctgccccg gaagctgccg
     2941 gacggagtgg gagacgttga tgccggtgga agttgcaagc tgaagagcgt agcagtccgg
     3001 ttcaatggga tcgtccaggc aaatccattt gagcggttcg tccaccactt tgaaatagtt
     3061 ttcatcaagg ttcaccaggt agcctttcag ggcgggctgg tggaaaagtt tgttcatcag
     3121 ggcgcatgcg gcctcctggt ccagatccag tgtcagggcg tcttccgcac gggcgtagtc
     3181 ggacagggag acgagcagga tttccgtctg ggcgtccatg cgtacggcag cccgttcatg
     3241 cagattttcc agatgttcca gatcctgttt ttcccggacg ggaatccagt cctgcttcag
     3301 ggaatggcgg tattcgaaat gtccctcgtt aatagtggaa acaagcctca tgaacagttc
     3361 tccgcggggc ggctgcgggg ggcgttcatt cacggattgg atgcggtcat accaggtgcc
     3421 gacttcacgg gagcgctccc attcatgaat cttgcgctgg accttttcca gatccgtaat
     3481 ggattccatg aattccgggt agggcaggcg tttcttgttg aaggcgtaag caatgtaatt
     3541 ccagaattcg aggatgtttg tgggaggaac gggccacaat tccagcgggt catagctgac
     3601 gatttcccat cggggattca ggcgcaccat atcatggtcg tgaatctcct gttcaatggc
     3661 gaaacggcgg tagcgctttt ccagtttgct gacatagtcc gcttccttgt cgtccagttc
     3721 gcgtcccagt ttctcttcca gaatatccag aagaggaaca tcattcagtt cgttcgggga
     3781 ttccggaagg tcctggccac ggtacatgcg ctccagcatg gcggcataca tggccgctcc
     3841 ggccagatcc tcgtcttccg tggaacatgc tccgaaccac ctgttgccct gaaggcgcag
     3901 attcacgcgg tgcacgccgt atgtgtcttc cactcggccc tgaatatata aatgattgcc
     3961 aaaaatttgc gtgactgcgc catctttctg gagcttctcg ccgcgcagcc ggatttcttc
     4021 cggaaaggca ttgagaaagt tc
//
LOCUS       DAOMSU010000196         4015 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2169_length_4015_cov_3.230808, whole genome shotgun sequence.
ACCESSION   DAOMSU010000196 DAOMSU010000000
VERSION     DAOMSU010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2169_length_4015_cov_3.230808"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1316
                     /locus_tag="PK077_08140"
     CDS             <1..1316
                     /locus_tag="PK077_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRN24307.1"
                     /translation="LAMLGFQVERLKTGTSPRVNGRSIDFSVCEIQPGDSPEALFSYR
                     SRDILKRDSEPFCTLNTWGDENFRMKQMPCWITYTNQTTHDIIRKNLKFSPLYSGIIE
                     GVGPRYCPSIEDKIVRFAEKNSHQVFLEPEGRSTEEFYLNGVSTSLPYSAQCDFIHTI
                     PGLENAQLIRPGYAVEYDFCPPTQLHPTLETKLVENLYFAGQINGTSGYEEAAAQGLM
                     AGANAALKTLGKPSLVLRRDQAYIGVMIDDLVTKGTDEPYRMFTSRAEFRLLLRQDNA
                     DLRLAPLAFEAGLITCEMARKAEDRKNAVEEGITKAAALKHEGVSLSTWLKRPGNDWT
                     LLPEELKKMFHVEHLDAIATDIQYEGHIERQKKQAEKIIRMENKTIPSDIDYEHVHAL
                     KKEAVIKLGNIRPATIGQASRIPGITPADIALLLVHLQKRSRQQT"
     gene            complement(1801..2871)
                     /gene="ribD"
                     /locus_tag="PK077_08145"
     CDS             complement(1801..2871)
                     /gene="ribD"
                     /locus_tag="PK077_08145"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HRN24308.1"
                     /translation="MLQNGVNTKNQDDCWMNMAIKLASNGIGLTSPNPCVGAVIVHEN
                     QVIGSGFHKKAGLPHAEREAIADGVANGNAPLFADSTLYVTLEPCSTSGKTPPCTDAI
                     LKHRFKRVVYGSEDPNPKHRGAAADILEQAGIKVTRGVLEKECDHLIRGFRVNMLEGR
                     PWVIAKSAMSLDGRISRSPERSQWLTNEKSRSFVHTLRAECDAVLTGGNTVRLDNPSL
                     TIRKPDRPVSPLKEQPWRIILTHDASSIPADSICLTDEFRDRTLVVENVFNYLELLKK
                     LYNNKKIGTILLEAGGCLVREFLKAGLVDEWVGFYAPIIIGGGTDGVAPGPFLEHEAH
                     LEQPVVNIFGDDVCIRGDICYR"
     gene            3277..>4015
                     /locus_tag="PK077_08150"
     CDS             3277..>4015
                     /locus_tag="PK077_08150"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24309.1"
                     /translation="MNIAAYCASRLREVGFEAVFDPGTPVSEEKGEIIEVSMHDFSPE
                     AAIWLAFCGAGEARCEEGHLLARKSGENGLTRVFTRKEAAIERLVYPWDLLEWQERVM
                     EKMEWEDFSGREGVYVMGTLRVGEGTVIMPGVVVEGAVWAGDGCRIGPNCHLRGCVSL
                     GNGCVVGQGVELKNCVIGDGTFIPHLSYAGDSIIGSDVNFGAGTVCSNFRHDGGEHRM
                     VAGGKLEFTGRNKLGAVIGDHVRLGANT"
BASE COUNT         1090 a          953 c          987 g          985 t
ORIGIN      
        1 atcttgccat gctgggtttt caggtggaaa ggcttaaaac aggaacttct ccccgcgtca
       61 atgggagatc catcgacttt tccgtctgcg aaatacagcc tggagactct ccggaagccc
      121 tgttcagcta ccgttcccgt gatatactga aacgtgattc cgaacccttt tgcactttga
      181 acacgtgggg agatgaaaat ttccggatga agcaaatgcc gtgctggatc acctacacca
      241 accagactac gcacgacatt atccggaaga atttaaaatt cagccccctt tacagcggta
      301 tcattgaagg cgtaggcccc cggtattgtc cttccataga agataaaatc gtgcgctttg
      361 cggaaaaaaa cagccatcag gtattcctgg aaccggaagg ccgcagtacg gaggaattct
      421 accttaacgg agtttctact tcactgccct actccgctca gtgtgacttc atccatacca
      481 ttcccggcct ggaaaacgcg caactgatcc gtccgggata tgcagtggaa tacgacttct
      541 gtcctcccac gcaattgcat cctacgctgg aaacaaaact ggtggaaaac ctgtactttg
      601 caggtcaaat caacggcact tccggctatg aagaagccgc agcgcaagga ttgatggcgg
      661 gagcgaacgc cgctctgaaa accctgggca aaccttctct ggtgctgcgc cgggatcagg
      721 cttacatcgg cgtcatgata gacgacctcg ttaccaaggg aaccgatgaa ccttaccgca
      781 tgtttaccag ccgggcagaa ttccgcctgt tgctgcgtca ggataatgca gacctgcggt
      841 tggcccctct ggcttttgaa gcgggtctta ttacctgtga aatggcccgg aaggctgaag
      901 acaggaaaaa cgccgttgaa gaaggaataa caaaagctgc cgcgcttaaa catgaaggag
      961 tttctctctc cacatggctt aaaaggcccg gcaatgactg gactctattg cctgaagaac
     1021 tgaaaaaaat gttccacgtg gaacatttgg atgccattgc tacggatatc cagtacgaag
     1081 ggcatattga aaggcagaaa aaacaggcgg aaaaaatcat acggatggaa aataaaacta
     1141 ttccctccga tattgattac gaacatgtcc atgccttaaa aaaagaggct gtcatcaagc
     1201 tgggcaacat acgtcctgca actatcgggc aggcctctcg aattcccgga attaccccgg
     1261 cagatatcgc cctgttgctt gtgcatctgc aaaaacgcag caggcaacaa acataatacc
     1321 ccgcgttttt tattcagaaa tttcaattta aggaggacca taggaggcgg gtattgaaaa
     1381 ataaatatgg cagggacatc cttgagccgt atatgatccc attatatttc cctgtcgcca
     1441 ttgcctggag tcgataaaaa gattcaatac tgttttctga atgtcttttt gcgcgacagc
     1501 agctcttccc ttcctccgaa caaccaccac tttctgcttg tcgtaaaaag aaatggaata
     1561 tcccttcagt attattgaat accggccttc ctgatttatc catcgcaaat cattaagaag
     1621 cgtcacacga tttcttcacg gattccattc aaatactgcc gataagaaaa agcagcattg
     1681 agcttactcc aagaggcaag ggaggccgga agggctgaat gacaaatcca ccgggcgaat
     1741 gaaaattacc ttcccaagtt taaaattctt cggataacca ataaacaaag acctcatcca
     1801 ttaccggtaa cagatatctc cccggataca tacatcatct ccaaaaatat ttacaacagg
     1861 ttgttcaaga tgggcttcat gctccagaaa cggaccgggc gctacgccgt ccgtaccgcc
     1921 gccgataatg atgggagcat aaaaccccac ccactcgtcg actaaaccgg ctttcagaaa
     1981 ttcccggacc agacagcctc ccgcttccag cagtatagtt cctatttttt tattattata
     2041 taattttttt aataattcca aataattgaa aacattctcc acaaccaaag tgcggtcacg
     2101 aaattcatct gttaaacaaa tggaatccgc aggtatggaa gaagcatcat gggttagaat
     2161 aatacgccag ggttgttcct tgaggggaga aacaggacga tccggtttcc taatggtcag
     2221 ggacggattg tccagacgca cggtattccc tccggtaaga accgcatcgc attcggcacg
     2281 caaggtatgt acaaaagatc tggatttttc attcgtcagc cattgggaac gttccggact
     2341 acgggaaatg cggccatcca gagacatggc gctttttgca attacccagg gacgcccttc
     2401 cagcatgttc accctgaacc ccctgattaa atgatcgcat tccttctcca gaaccccgcg
     2461 ggtaaccttg atgccggcct gttcaagaat atcagccgca gcgccgcggt gtttgggatt
     2521 aggatcttcc gaaccataaa ccacccgttt aaaacgatgt ttcaaaatgg catccgtaca
     2581 aggcggtgtt ttcccggaag tactgcatgg ttccagagta acatacaaag ttgagtccgc
     2641 gaacaggggg gcattgccat tcgccactcc gtcggcaata gcctctcttt ccgcatgagg
     2701 caagccggct tttttatgaa atcccgaacc aatcacctgg ttttcatgca caatgactgc
     2761 ccccacgcag ggattcggac tcgtaagtcc gattccattt gaggccagtt ttatggccat
     2821 gttcatccaa cagtcatcct gatttttcgt attcacgccg ttttgtagca tgaaaaccgg
     2881 aagaaggaca atcacgttgt gtgattgaag aatattaggt ttaacaatat taaattcctt
     2941 cttctgctgt gtgcccagtg tgaataagat tttaaatgac tgtaaatgag agcattgagt
     3001 atcatttcaa atgttaataa atttggcctg caaatttgtg aataatatct gaataagtta
     3061 ttcacatcat actcacagtt aatcaacctg gcctttcaca aggttattgg caggtgcggc
     3121 tgtaaacaga aacggcattc ctgcttgcag aaaacccgga ggaaagcata ccatcctttt
     3181 gtgaaggctg ccagattgat ttacattccg gagagggatc atacttttcc cgctacaagc
     3241 cctgaaaggc ccttctacgg cttttccgtg gctggaatga atatagccgc ttactgtgct
     3301 tcccgccttc gggaagtggg ctttgaagcg gtttttgacc cgggaacgcc ggtttcagaa
     3361 gaaaaagggg aaatcataga agtgtccatg catgattttt ccccggaggc ggccatatgg
     3421 cttgcctttt gcggggcagg ggaagcaagg tgtgaagaag ggcatttgct ggcccggaaa
     3481 agcggggaaa acgggttgac gcgggtattc acgcggaagg aagcagcgat agagcgcctc
     3541 gtttatccct gggatcttct ggaatggcag gaaagagtga tggagaagat ggagtgggag
     3601 gatttctccg gaagggaagg ggtttatgtc atgggaacgc tccgggtagg ggaaggaacc
     3661 gtcatcatgc ccggcgtggt tgtagaggga gccgtatggg cgggtgatgg ctgccgcata
     3721 gggcctaatt gccatttaag ggggtgtgta tctttgggga atggctgtgt agtagggcag
     3781 ggagtggagc tgaaaaattg tgtcatcggc gacggcacat ttattcctca tttgagttat
     3841 gcgggggatt ccattattgg cagtgacgtt aatttcggcg ccggaacggt atgttccaat
     3901 tttcgccatg atggcggcga acacagaatg gtggcgggag gaaagctgga atttactgga
     3961 agaaacaagc ttggcgctgt cattggagac catgttcggc tgggtgccaa taccg
//
LOCUS       DAOMSU010000197         4006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2177_length_4006_cov_3.243736, whole genome shotgun sequence.
ACCESSION   DAOMSU010000197 DAOMSU010000000
VERSION     DAOMSU010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4006
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2177_length_4006_cov_3.243736"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="PK077_08155"
     CDS             <1..1167
                     /locus_tag="PK077_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRN24310.1"
                     /translation="EREEREREKKTNALFLYSRAIGSASDVPSLISVAAGQISQIMGV
                     RLAVMTPEGGGGKLRLWETGGEYAMDEKEWSVAVWCMEHRRPAGRFTDTIPVAEGFYL
                     PMMSGERCMGVLGVRPEEGECLTAGQKDLLESMGAQLAMALEREELRAERARTRLMEE
                     SEKLHRSLLDSVSHEFKTPLAVIEGGCEKLAGRAASAPEEREEYGEILLAARRLRRLV
                     KNLLDVSRLESGALKPKLDWCDLGDVIEGALAATREARRNHPVSVSLPPDYPLVKADF
                     SLMEQVLVNLLLNACVHTPEGTSVTLQGGADSVRGQVWLDIHDMGPGIPPERAETIFE
                     RFRTTRPGGLGLGLPIVRGFMEAQRGAVSLVPVSAGTCFRLVLPLVEHDSVPEE"
     gene            1181..1879
                     /locus_tag="PK077_08160"
     CDS             1181..1879
                     /locus_tag="PK077_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009965140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRN24311.1"
                     /translation="MSNFPADILIIDDERQIRRLLNLTLTGAGYHVRECENGQLGLSE
                     TALKRPDAIILDLGLPDINGLEVLKQLREWTQVPILILTAWDREDEKVDALDAGADDY
                     LTKPFSGRELLARLRVMLRRNRPDREPSVFRLGPIVVDLSSRRVEKGKEEIHLTSKEY
                     GILRLLLLHQGKVMTHRQLLREIWGPQHEEDTHYLRVHIAHLRQKLGDSDPENRIIRA
                     ESGLGYRIVADGAE"
     gene            2033..2812
                     /locus_tag="PK077_08165"
     CDS             2033..2812
                     /locus_tag="PK077_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRN24312.1"
                     /translation="MKIKYNARNVGAAALGCAMIAGTVCAGEETVSPSIASLTNMQEA
                     AIPSTDTDWAFSFSAGWSSKYVTEGLDCLPGSGIWEVAPSISWKNLTFSAWYAGGDSA
                     NYDELDLVLGYAWNLGKWTVNPWYEHQFYFTQDYNVVNPALTVSYAVTDWMAVGAETQ
                     VKVEHQDFEAYYSAFVRLEWSPLENVTVVPMVRYGYNGGYNVDYDDGSNCIDWSLGVT
                     WRFAEHYTLSGSVNYSQAATVLRHRDAGDEFWVGFRLGVEF"
     gene            2925..>4006
                     /gene="kdpA"
                     /locus_tag="PK077_08170"
     CDS             2925..>4006
                     /gene="kdpA"
                     /locus_tag="PK077_08170"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HRN24313.1"
                     /translation="MSSHDLLIIVLFIGILVSFTPFLGKWLASVLMGRQTWLSPVLGP
                     VERCVYRMAGVDPTREMDWKKYLTAVLLFNAAGFLVLFLSLLCQKWLPLNPAGTENMR
                     WDIALNTAISFVTNTNWQFYSGEGPEGVSYFVQMTGLGVQNFVSAGTGIAVMSALIRG
                     LERRCASTLGNFWTDLTRSMLYFLVPVSTVIALLLVSQGVVQSFDGPIAVPGMDGVEQ
                     MIPSGPAASQIAIKQLGTNGGGFFGNNSTHPFENPTPFSNMVEMLSLLLAGCACPYAY
                     GVMIGKKRQGWIIFGAMMILLVAAIVLSQWAEHAGNPLFPGMEMLEGKEVRLGVTNSS
                     LWSVATTASSNGSVNCMHSSMSPLGGG"
BASE COUNT          823 a         1024 c         1242 g          917 t
ORIGIN      
        1 gagcgggaag aacgggaacg ggaaaaaaag accaatgccc tgttcctgta ttcccgggcc
       61 attggatccg cttcggacgt accttccctc atttccgtcg cggcgggaca gatcagccag
      121 attatggggg tgcgcctggc tgtcatgacg ccggaaggag gcggcggtaa gctgaggctt
      181 tgggagaccg gcggagagta cgccatggat gagaaggaat ggagcgtggc agtctggtgt
      241 atggaacacc gccgccccgc aggaaggttt acggatacga tcccggtagc ggaagggttt
      301 taccttccca tgatgtccgg ggagcgttgc atgggcgtac tgggcgtaag gccagaggaa
      361 ggggagtgcc tgacggccgg gcagaaggat ttgctggaaa gcatgggagc gcagctggcc
      421 atggcgctgg agcgtgaaga attgagggcg gaacgggcgc gtacgcggct catggaagaa
      481 tcggaaaaac tgcaccgatc cctgttggac agtgtttccc atgagttcaa aacgcctttg
      541 gccgtgattg agggcggctg tgaaaaattg gccggacgtg ccgcgtccgc accggaggag
      601 cgggaagaat acggggaaat tttactggcc gcgcgccgtc tgcgccgcct ggtgaagaat
      661 ttgctggatg tgagccgcct ggaatccggc gctttgaaac ccaaactgga ctggtgcgat
      721 ctgggggatg ttattgaagg ggctctggct gctaccaggg aagcacgccg gaatcatccc
      781 gtgtctgttt ccctgccgcc ggactatccg ctggtgaagg cggatttttc cctgatggaa
      841 caggtactgg tcaatctgct gttgaatgcc tgcgtacata ctcctgaggg aacctctgtt
      901 acgttgcaag gtggtgccga ctccgtgcgc ggccaggtct ggctggatat tcatgacatg
      961 gggccgggca ttcctcccga acgggcggaa accatttttg agcgcttccg gacgacgcgg
     1021 cccggtggtt tgggcctggg gctccccatt gtgcgcgggt tcatggaagc tcagcgcggt
     1081 gccgtctccc tggttcccgt ttccgccgga acatgtttcc ggcttgtcct tcccctggtg
     1141 gagcacgata gtgttcctga agagtaattc ctcctgcctc atgagcaact ttcctgcaga
     1201 tatcctgatt attgacgatg aacggcagat acgccgtctt ctgaacctga cgctgaccgg
     1261 cgcaggctac catgtccgcg agtgtgaaaa cgggcagctt ggcttgagtg agaccgctct
     1321 gaagcggccg gatgccatta ttctggatct ggggcttccg gacattaacg gcttggaagt
     1381 gctgaaacag cttcgggagt ggactcaggt tcctatcctg attctaacgg cctgggacag
     1441 ggaggatgaa aaggtggatg ccttggatgc cggggcggat gattacctga ccaagccgtt
     1501 cagcggccgt gaattgctgg cgcgcctgcg cgtgatgctt cggcgcaatc gtccggaccg
     1561 tgagccgtcc gtattccggc tgggtcccat tgtcgtggat ctgagttccc ggcgcgtgga
     1621 gaaggggaaa gaggaaattc atttaacctc caaagagtac ggaattctgc gcctgcttct
     1681 gctccatcag ggcaaggtga tgacccaccg ccagctgctc cgtgaaattt ggggccccca
     1741 gcatgaggag gacacgcact acctgcgggt gcacattgcc cacctgcgcc aaaagctggg
     1801 ggattccgat ccggaaaacc gcattatccg cgcggaatcc ggcctgggtt acagaattgt
     1861 ggcggatgga gccgagtagc ccttgtccca gttttactgg gctggaaaaa atagcggcac
     1921 attatttgcg gcagccggaa aatatatttt ttcataacgg ttggagaatg tggcttaact
     1981 tcacgcagtc caacatgctg tgtaggatcc aagacgtatt caatacgtct ttatgaagat
     2041 caagtacaac gcaagaaacg taggagccgc agccctgggc tgcgccatga tagcaggaac
     2101 cgtctgcgca ggagaggaaa cggtttctcc gtcgatagcc tccctgacaa acatgcagga
     2161 ggccgccata ccttcgacag acacggattg ggccttttcc ttctcggcgg ggtggtccag
     2221 taagtacgta acggaaggtc tggactgcct gccgggcagc ggcatctggg aagtggctcc
     2281 gagcatttct tggaagaatc ttacgttcag cgcctggtat gccggaggag attccgccaa
     2341 ttacgatgaa ctggacctgg ttctgggtta cgcctggaat ctggggaagt ggactgtcaa
     2401 tccctggtat gagcaccaat tctattttac gcaggattat aatgtggtca atccggcgct
     2461 gacggtctcc tatgccgtaa cggattggat ggctgtagga gcggaaaccc aggtgaaagt
     2521 ggaacaccag gattttgaag cctattacag cgcctttgtg cggttggagt ggtcccccct
     2581 ggaaaatgtg accgtggttc ccatggtcag gtacgggtac aacggcggtt ataacgtgga
     2641 ctatgatgac ggctccaact gcattgactg gagcctgggc gtgacatggc ggtttgcaga
     2701 acattatacg ctttccggct ccgtcaatta ttcccaggcg gctacggtgc tgcgtcacag
     2761 ggatgccgga gacgaattct gggtgggatt ccgcctgggc gtggagtttt aacggcgcac
     2821 cggcgttaaa aaacgggctt ccggagagga attaataatt tcttaatgtc cggaggccct
     2881 ttgttaacta cccggaattt ccagaatatg cgtagagttg cgttgtgtcc tcacacgact
     2941 tgttaatcat tgtcctgttc ataggcatac tggtctcttt caccccgttt ctcggaaaat
     3001 ggctggcaag tgttttgatg ggaaggcaga catggctttc cccggttctg ggtcccgtgg
     3061 agcgctgcgt ctaccgtatg gccggagtgg accccacccg cgaaatggac tggaagaagt
     3121 atttgacggc cgttcttttg tttaacgccg ctggattcct cgtccttttc ctttccctgc
     3181 tgtgccagaa atggctgcct ctgaatcccg ccggaactga aaatatgcgg tgggatatag
     3241 ccttgaatac agccatcagc ttcgtgacca acactaactg gcagttctat tcaggggaag
     3301 ggccggaagg cgtcagttat tttgttcaaa tgaccgggct gggcgtgcaa aatttcgtca
     3361 gcgccggtac gggaattgcc gtcatgtccg ccctgatccg cgggctggaa aggagatgcg
     3421 cttctactct ggggaacttt tggacggatc tgacgcgcag catgttgtat tttctggtcc
     3481 ctgtttccac ggtcattgct cttctgctgg tctcccaggg tgtggtgcag tcctttgacg
     3541 gacccattgc cgtgccgggc atggatggcg tggaacagat gatacccagt ggcccggcgg
     3601 catcccagat agccatcaaa cagctcggaa caaacggcgg cggctttttc gggaacaaca
     3661 gcacacatcc ttttgaaaac cccacgccct tcagcaatat ggttgaaatg ctctccctgc
     3721 ttctggccgg ctgcgcctgc ccatacgcct atggcgtcat gatcggaaaa aagaggcagg
     3781 ggtggattat cttcggagcc atgatgattc tgcttgtggc ggccattgtc ttgtcccagt
     3841 gggcggaaca tgcgggaaac ccgcttttcc cgggcatgga aatgctggaa ggcaaggaag
     3901 tgcgcctggg cgttacgaac agttccctct ggtccgtcgc tacgacggca tcttccaacg
     3961 ggtccgtgaa ctgcatgcat tccagcatgt ccccgctggg cggcgg
//
LOCUS       DAOMSU010000198         3971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2205_length_3971_cov_2.446118, whole genome shotgun sequence.
ACCESSION   DAOMSU010000198 DAOMSU010000000
VERSION     DAOMSU010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2205_length_3971_cov_2.446118"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..1121)
                     /locus_tag="PK077_08175"
     CDS             complement(162..1121)
                     /locus_tag="PK077_08175"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HRN24314.1"
                     /translation="MIFGTLPGGLPVELFELSSDKLTVRISNYGGRIISVVKDGMDMI
                     VGPKRFEDMLKDTCYCGAICGRVANRIAKGRFSIDGDSRTVAVNNGPNHLHGGIQGFD
                     SKVWQVQEAGRNTLVLTLHSADGEENYPGNLEVVATYTVVEETLHLRLEAYADAATIV
                     NLTNHAYWNLSGKPTIDSMTLEVRASAYTPVDATNIPDGRILPVEGTDFDLRKAALLG
                     ERNSSAHPDTAAGLDHNYVLDSEPGDKIAAILLDPESRHRLIVATDAPGLQVYTGEYL
                     PQARQGIALEAQGFPNAVNTPHFPSVILRPGQSAMRSITWTVR"
     gene            complement(1156..1635)
                     /locus_tag="PK077_08180"
     CDS             complement(1156..1635)
                     /locus_tag="PK077_08180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HRN24315.1"
                     /translation="MKTASILTIGCAAMALSITSCTNFGSPNTYNLNEIGGAQETYTG
                     TVTSVENVKIQANNANTGTGIGAVAGGLTGAMFGGGNAKFATAAGGAILGGIAGNQID
                     KAVNNTTGERITVRLDQKRNGTRNYTVVQVASRNNPIQVGQRVRVIIGNNGSRVLAY"
     gene            complement(1781..2023)
                     /locus_tag="PK077_08185"
     CDS             complement(1781..2023)
                     /locus_tag="PK077_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24316.1"
                     /translation="MKKILAVAAFAFAFSVNGYAQEAAAPAESPAPAAQKCCKKDKKC
                     PKKCAKEGKCPEKCGQPCSKGEKPCDCPKKGEAPKA"
     gene            2261..>3971
                     /locus_tag="PK077_08190"
     CDS             2261..>3971
                     /locus_tag="PK077_08190"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRN24317.1"
                     /translation="MSSAKEPVTRRFGNGLTVLIKEDKSHPVVSLQYWVGTGSMNEGH
                     WQGSGLSHLLEHLVFKGTAHFSGQDLARKVQERGGHWNAYTSVNRTVYYIDGPAESWQ
                     IFLNLLTELVFFPTFPEDEMEREKEVVRREMAMYADDPDSVAYQLLMQTLYLKHPRRW
                     PVLGERAAFDCLTRQDVLDYHASRYVPNNVVLSIAGDVDAAEILSHLELLVEDLKSRP
                     LNREPIPHEPHQFGSRRVRKEFAVPYSKLHLAWRLPCSAHPDTPALSALSSILGGGRS
                     ARFYEKFHDRLGLVYGIEVHSNQSETDEGAFTISMDVDRAQRDKVRDLVLQELRNLAE
                     EDFTEDLKRVCKQTRVSRLRRRSSASGMASEMGADWFGARNLNLSSEWQEAIERVTTE
                     DLHRVCSTWLSSPNVTEVSLDPSGSNSGEEEGAASAAGTSLSEHVLGNGMKVVIREDH
                     RLPLAYACMAFKAGCRAENEHDAGVTDLMAECLLKGTSTRSAADIARFLEDIGGAINT
                     STGNNSLSVGCQVLAEDLDAGLELMADVVMNPSFPEDAFLKEQESFVADAEEDMEDPL
                     SVAF"
BASE COUNT          736 a         1046 c         1281 g          908 t
ORIGIN      
        1 ttgtaagccg ttccctggaa cgcatcgcgg aagggctcca aaaggtagcg gcggaagggc
       61 tttacgcggc ctcctgatgc cgcgtgcctg cacaggaaag cccgcagcgg gcgttgttga
      121 agcgccgttt tttgttcgga aacgggattc aggcatgccg cctatctgac agtccaggta
      181 atgctgcgca tggcggattg gccgggacgc agaatgacgc tggggaagtg aggggtattg
      241 acggcgttgg ggaagccctg ggcttccagc gctattccct ggcgcgcctg gggcaggtat
      301 tccccggtat acacctgaag cccgggagcg tccgttgcca caatcaggcg gtgccgggat
      361 tccgggtcaa gaaggatggc tgcaatcttg tcccccggtt cggagtccag cacgtagttg
      421 tggtccagtc cggcggcggt atccgggtgg gcggaggagt tgcgttcccc cagcagggcc
      481 gctttccgca ggtcaaagtc cgtcccttcc acgggaagga tacggccgtc ggggatattg
      541 gtcgcgtcca ccggagtata ggcggaggcc cgtacctcca gagtcatgga atcaatggta
      601 ggcttgccgg acaggttcca gtaagcgtga ttggtgaggt tgacgattgt ggctgcgtcc
      661 gcatacgcct ccagacggag atgaagggtt tcttccacca cggtgtaggt ggctaccact
      721 tccaggttgc cggggtaatt ttcctcaccg tctgcggagt gcagggtcag aaccagcgta
      781 ttgcgcccgg cttcctgtac ctgccagact ttgctgtcga atccctggat gccgccgtgc
      841 aggtggttgg ggccgttgtt gacggccacg gtgcgggaat ccccgtcaat ggagaacctg
      901 ccttttgcga tgcgattcgc gacacggccg caaatcgcac cgcaataaca ggtatctttc
      961 agcatgtctt caaagcgctt gggccctacg atcatgtcca tgccgtcctt gacgacggag
     1021 atgatgcgtc ccccgtagtt gctgatgcgt accgtcagct tgtcggaaga cagctcaaac
     1081 aattctacag gtaagccgcc gggtaaggtg ccgaagatca tgccggtata tagctggtca
     1141 gtagtggatt tccaattaat aggccagcac gcgggaaccg ttgttgccga tgattacgcg
     1201 cacgcgctgt ccgacctgaa tcgggttgtt cctgctggcg acttggacaa ccgtgtagtt
     1261 gcgggtgccg ttccttttct ggtccagacg cacggtgatg cgttccccgg tcgtgttgtt
     1321 gacggccttg tcaatctggt tgccggcaat gccgcccaga atggcgccgc ccgccgccgt
     1381 cgcgaatttg gcgttgccgc ccccgaacat ggcgccggtc aacccgcccg ccacggcgcc
     1441 gattccggtg ccggtgttgg cgttattggc ctggatcttg acgttctcca cgctggtgac
     1501 cgtgccggta taggtttcct gggctccgcc gatttcattc aggttatagg tgttcgggga
     1561 accgaagttg gtgcaggaag taatggacag cgccatggct gcacagccaa tggtaagaat
     1621 ggaagctgtt ttcatgatga cgatttataa aatgggttga aggttaagat caagtaattt
     1681 cgcagtcaac ggaaggggca cggttgccct cttccggcgg agaaaaccct tgccggagag
     1741 ttccggcaag ggtttgtatt tattttaacg gttcgttgag tcaggccttg ggggcttctc
     1801 cctttttggg gcagtcacag ggcttttccc ctttgctgca aggctggccg cacttttctg
     1861 ggcatttgcc ttccttggcg catttcttgg ggcacttttt gtctttcttg cagcacttct
     1921 gggcagcggg ggcgggggac tccgccggag cggcggcttc ctgagcgtaa ccgttgacac
     1981 tgaaggcgaa agcaaaggct gctacagcga ggatcttttt catataatga tatgtgaggt
     2041 tgtgtggtgt tggtaatgca gcggcagagg agctgccgtt ctatgactga tagagcggca
     2101 gggagtgatt tttgttcaaa aaatacgcgg agaggattct ttttagggcg gtttttttga
     2161 tgaggacgcg cttttggcct ccggggtaag tttgacagtc cgggggcacg tacggagcgc
     2221 cggcttgcat ggggcgcctt cttgaggcac ggtagggaac atgagttcag ccaaggaacc
     2281 tgtgacgcgc cgttttggaa atggtttgac ggttttgatt aaggaggaca agtctcatcc
     2341 cgtagtgtcc ctccagtact gggttggcac cgggtccatg aatgaggggc actggcaggg
     2401 gagcggactt tcccatttgc tggagcatct ggtgtttaag ggaacggcgc atttctccgg
     2461 gcaggatctg gcccgcaagg tgcaggaacg cggcggccac tggaatgcgt acaccagcgt
     2521 gaaccgcacc gtgtactata tagacggccc tgcggagtcc tggcagattt tcctgaatct
     2581 tctgacggag cttgtatttt tccccacatt cccggaagac gagatggagc gggagaagga
     2641 ggtggtgcgc cgggagatgg ccatgtatgc ggatgatccc gattccgtgg cctaccagct
     2701 tctgatgcag acgctgtatc tcaagcatcc ccgccgctgg cccgtgctgg gtgagcgcgc
     2761 cgcctttgac tgcctgacgc ggcaggacgt gctggattat catgccagcc gctatgttcc
     2821 gaataacgtg gttctttcca ttgcgggaga cgtggatgcc gcggagattc tttcccatct
     2881 ggaattgctg gtggaagact tgaaatcccg tcccctgaac cgggagccca ttccccatga
     2941 gcctcaccag ttcggttccc gcagggtgcg gaaggagttt gccgtgcctt actccaagct
     3001 gcaccttgcc tggcgcctgc cctgttccgc ccatccggat acgccggctc tttccgcgct
     3061 gtccagcatt ctgggaggcg ggcgttccgc gcgtttttat gaaaagtttc atgaccgcct
     3121 gggcctggtg tacggcattg aagtgcattc caaccagtct gagacggatg agggggcgtt
     3181 taccatcagc atggacgtgg accgcgccca gcgcgacaaa gtgcgggatc tggtgcttca
     3241 ggagcttcgc aatctggcgg aagaggattt tacggaggac ttgaagagag tttgcaaaca
     3301 gacccgggtc agtcgcctgc gccgcaggag ttccgcttcc gggatggcct cggaaatggg
     3361 agcggactgg ttcggcgcgc gcaatttgaa cctgtcttcc gaatggcagg aggctattga
     3421 acgggtgact acggaagatt tgcaccgcgt ttgttccacc tggctgtctt ccccaaatgt
     3481 gacggaagtc agcctggacc cctccggcag caactccggg gaagaagaag gggcagcctc
     3541 cgctgcggga acgtccctga gcgagcatgt tcttggcaac ggcatgaagg tggtgatccg
     3601 tgaagaccat cgcctgccgc tggcttatgc ctgcatggcg ttcaaggccg gatgccgtgc
     3661 ggagaatgag catgacgccg gcgtgacgga cctgatggcc gagtgcctgc tgaaaggaac
     3721 ctccacccgt tccgcagcgg atatagcccg ttttctggag gacatcgggg gagccatcaa
     3781 cacgtccacc ggtaacaatt ccctgagcgt ggggtgtcag gtcctggcgg aagacctgga
     3841 cgccggactg gagctgatgg cggacgtagt catgaatcca tctttcccgg aagacgcctt
     3901 tctgaaggaa caggagtctt ttgtggcgga tgcggaggag gatatggaag accctctttc
     3961 cgtggcgttc c
//
LOCUS       DAOMSU010000199         3971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2206_length_3971_cov_2.326353, whole genome shotgun sequence.
ACCESSION   DAOMSU010000199 DAOMSU010000000
VERSION     DAOMSU010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2206_length_3971_cov_2.326353"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="PK077_08195"
     CDS             <1..1536
                     /locus_tag="PK077_08195"
                     /inference="COORDINATES: protein motif:HMM:NF019880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRN24318.1"
                     /translation="AEKAGISIVEEVYDAAEERRRKARTALLVAHKKAAGFFHRLLLC
                     SPKAAEARAYLNSRGYGADMAKRWQVGWAPDSSREFLAWAHREGIPDQVLIDSGLANR
                     GERGDLYARFRDRLMFPINNVYGECVAFSGRILRPQENVGKYVNSPETAIFKKGDVVF
                     ALDKARGPMGKSGKALLCEGQIDVIACHEAGISFAVAPLGTAFTPEHARILSRYASRI
                     VLCFDADKAGMAAADRAFRELAPFGKDIYLVNLPAGDDPDSFMKREGKEAFSGMVERA
                     RSFFEVRIERARESGLLDDAASSAAFAREMTALLACMSDPVARDLATADIATRMRMAA
                     DNLRGAVRMAGRRKGQEQAQSAERKTAEEVPQHPPVKMDRAVAVLCELALQNSRAQGL
                     IVDRIEELLEPMRLLQGGGILKKILARLPSPDSPAAVQAFLVSLPQPERDALGMLNLE
                     PIPIPDVDRSVQEACSGIAKAALERHIASLMAELADPSTDAARRLELSKLSVDLKRLL
                     GTM"
     gene            1596..2396
                     /locus_tag="PK077_08200"
                     /pseudo
     CDS             1596..2396
                     /locus_tag="PK077_08200"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            2946..3662
                     /gene="rpoD"
                     /locus_tag="PK077_08205"
     CDS             2946..3662
                     /gene="rpoD"
                     /locus_tag="PK077_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HRN24319.1"
                     /translation="MVEANLRLVISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEYR
                     RGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLMRVQKQLVQEYGREPTP
                     EEIAEEIHLPVERVRSVLKMAQQPISLQAPVGDSDDTSFGDFIEDKAAENPMEEASFS
                     FLKEKIKDVLDTLTDREREVIEQRFGLRDGSPRTLEEVGRQFSVTRERIRQIEAKALR
                     KLRHPTRISKIKGFLEMTES"
     gene            3731..>3971
                     /locus_tag="PK077_08210"
     CDS             3731..>3971
                     /locus_tag="PK077_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013586.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HRN24320.1"
                     /translation="MFMLLVSCSGSREFKPAFNQPLQPVMQPGFVYVDQVAPLVKTNL
                     KYAGYDNFVGRPLDGYRGRRAILRTQAAQALKKVSE"
BASE COUNT          914 a         1090 c         1174 g          793 t
ORIGIN      
        1 gcggaaaaag ccggaatctc cattgtggag gaggtctatg acgcagcgga ggaaaggcgg
       61 cgcaaggcac gcacggcttt gctggtggcc cacaagaagg cggctgggtt tttccacagg
      121 ctcctgcttt gttccccgaa ggctgcggag gccagggctt acctgaattc ccgcggatac
      181 ggggcagaca tggcgaaacg ctggcaggta gggtgggctc ctgactcctc gcgggagttt
      241 ctggcctggg cccacaggga gggtatcccg gaccaggtgc tgatagattc cggtctcgcc
      301 aaccgcggag aacggggaga tttgtacgcc cgcttccggg acaggctgat gttccccatc
      361 aacaatgtgt acggagaatg tgtggcgttt tccggccgca ttctgcggcc acaggaaaac
      421 gtaggcaagt acgttaattc cccggagacg gccattttca aaaaggggga cgtggtgttt
      481 gccctggaca aggcgcgcgg ccctatggga aaaagcggca aagccctgtt gtgcgagggg
      541 cagattgacg tcattgcctg ccacgaggcg ggtatttctt ttgccgtagc ccctttgggg
      601 acggcgttta ctccggagca tgcccggatt ctttcacgct atgcttcacg gatagtgttg
      661 tgttttgacg cggacaaggc gggcatggct gcggcggacc gggctttccg tgaactggcc
      721 ccttttggca aggatattta cctggtgaac cttcctgccg gggacgatcc ggattctttc
      781 atgaagaggg aggggaaaga ggctttttct gggatggtgg aacgggcccg ttccttcttt
      841 gaggttcgca ttgaacgggc cagggagagc ggtttgttgg atgacgccgc ttccagcgcc
      901 gcttttgccc gtgagatgac ggccctgctg gcctgcatga gcgatccggt ggcccgggac
      961 ctggctacgg ctgacatagc cacgcgcatg cgcatggctg cagataattt gcgcggcgcc
     1021 gtcagaatgg ccggacgccg gaaggggcag gaacaggccc agtccgcaga gcgtaagacg
     1081 gctgaggagg ttccgcagca tcctcctgtg aaaatggatc gcgccgtggc cgtgctctgt
     1141 gaactggctc ttcagaattc ccgtgcccag gggcttatcg tggaccgtat tgaagaattg
     1201 ctggagccga tgcggctgct gcagggcgga ggcatcttaa aaaaaattct ggccaggctg
     1261 ccttctccgg acagccccgc ggccgttcag gcttttctgg tgtctctgcc tcagccggaa
     1321 cgggatgccc tgggcatgct gaatctggag cccataccta ttcccgatgt ggacaggagt
     1381 gtacaggaag cctgctccgg cattgcgaag gccgctttgg agaggcacat cgcctccctg
     1441 atggcggaac tggcggatcc ctccacggat gctgccagaa ggctggaatt gtccaaactt
     1501 agtgttgact tgaagcgttt gctgggtaca atgtagtgcc tcgatacctc cgggtattga
     1561 tataatggtg aattgtgccc tcttcttttc aagatatgtc caaatccggt gacccatccc
     1621 cctcttctcc caagaagact tccgccaaca aaaaagcttc tgaatccaag gaaaaaatag
     1681 cggcccggcc cgccgcaaaa aattccaaaa ccgctccgaa ggcggctcct gtcaaaaaaa
     1741 actctgccaa ggctgccgct ccggctgcgg aaaaggccgc cgccaagccc attgcggaaa
     1801 aaaaggcccc ggcaaaaaaa gcgtctccgg cttctgcctc caagaaggcg gcgccggccg
     1861 ccaaaaagac tccggcaaag gcgcctgaag ctgttcctgc caagaaagct ccagccaagg
     1921 ctgcggagaa gaagccggct gagaagaagg ccgccaaacc tgccgcgaag gaggaaacgc
     1981 ccaaaaagtt aagcgaaata ctggaagagg aacgcaagag ggctgccagc cgtaagatca
     2041 cgaatccgat tgacgctccg gaaattcagg aaaaaatccg tgaacttatc aagctcgcca
     2101 aggaacaggg gtatctgact tttgacgata ttaatgactc ccttcctaac gacattgtcg
     2161 atcagcagga ttacgaggcc atcatggacc gcctgcgcag catggcgttt gacatcattg
     2221 acgcgtctga tgtggacagc tacacggacc gcacccgcat cagcacggaa gaagaggacg
     2281 aagaggaaaa gctcgaagcc aagatggaca ttctggatga tcccgtccgc atgtacctga
     2341 agcaaatggg ccaggtttcc ctgcttaccc gtgaagagga agtggccatt tccaagagaa
     2401 ttgaggacgc ggaacagaac gtccagcgct gtgtgcaccg cttcggtttc atcgcgaatg
     2461 cctatctgga cgtagcctat cgcctgctcg acaatgagga acgctttgac cgcgttatcc
     2521 ttgacaaaaa gatagactcc cgtgagcgct atatgaaagg gctggcccag ctctgcgccc
     2581 agatacagca gacccatcag gacgcatccg gttccttccg caagctgtac cgcagcaagg
     2641 aggcggccaa gtccgtcaag gcccgccagg ccgaattcga caaagttgcc ggcgctctgg
     2701 tgaagttctt cggtcgcctg tatttcaagc acaaggtgat tgaggatttc tgttccatga
     2761 ttgacgaggc ccgcgaccgc gtgctccgca tgcagaagaa ggttgccctg gaaccggaca
     2821 acaaggaact caaggaacat ctggcagagc tggagcttcg catgtggatg actgcggatg
     2881 agatgggaga tgcctaccag gaactccgca aatggcttcg cgaagcccgc agggccaagg
     2941 atgaaatggt ggaggccaat ctgcgcctgg tcatttccat tgccaaaaaa tataccaacc
     3001 gcggcctctc cttcctggat ctgattcagg aaggcaacat gggcctgatg aaagcggtgg
     3061 aaaaatttga ataccgccgc ggctacaaat tctccaccta tgctacctgg tggattcgcc
     3121 aggccatcac ccgctccatt gcagaccagg cccgcaccat ccgcattccc gtgcacatga
     3181 ttgaaaccat caataaattg atgcgcgtgc aaaagcagct ggtgcaggag tatggtaggg
     3241 aacccactcc ggaagaaatc gcggaagaaa tccatctgcc tgtggaacgc gtgcgttccg
     3301 ttttgaaaat ggcccagcag cctatttccc tgcaagcccc cgtgggggat tccgacgata
     3361 cctccttcgg tgactttatt gaagacaagg cggcggaaaa tcccatggag gaagcctcct
     3421 tctctttcct gaaagaaaaa atcaaggatg ttctggacac cctcacggat cgtgaacgcg
     3481 aagtcattga acagcgcttc ggcctccggg acggcagtcc ccgcactctg gaggaagtgg
     3541 gccgccagtt tagcgtcacc cgcgaacgca ttcgccagat tgaagccaag gccctccgca
     3601 agctgcgcca tcccacgcgc atcagcaaga tcaagggatt cctggaaatg acggaatcct
     3661 gaccggattt gcgccgcatg ccgtgctacg cttcgcggtc atgaagactt gcgtagctgc
     3721 ggtatgcggc atgttcatgt tactggtttc ctgttccggc agcagggaat tcaaaccggc
     3781 attcaatcag ccccttcagc ccgtgatgca gccgggcttt gtatatgtgg accaggtggc
     3841 tccgctggtg aagactaatt tgaaatatgc cggatatgat aattttgtag ggcgtccgct
     3901 ggacggttac cgcggccggc gcgccatcct gcgcacccag gcggcgcagg cgttgaaaaa
     3961 agtctcggaa g
//
LOCUS       DAOMSU010000200         3958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2226_length_3958_cov_2.527030, whole genome shotgun sequence.
ACCESSION   DAOMSU010000200 DAOMSU010000000
VERSION     DAOMSU010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2226_length_3958_cov_2.527030"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..>2005
                     /locus_tag="PK077_08215"
     CDS             342..>2005
                     /locus_tag="PK077_08215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009958438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRN24321.1"
                     /translation="MNHPYLDPAFLVSWSRLTPEAIRPDITEAISRAKENIRAICDQP
                     LDSLTYESTFGALEKASEDLHLGWGRIMHLDSVNDEPAQREAIGEMLPEVVTFSSSVP
                     LNPRLWTVLKAAASCDWVEDLSPVRQRFIQETLADFRESGADLPDDVKPEYAEIEAQL
                     SLKTKKFAENVLDSTNAWELIVEDEAELSGLPDSVKEAARLDALANGYGTEEEPRWRF
                     TQKFTSLQPVMQFADSDSLRRRMWEGSCSIGKGGEYDNEALIAEILELRDRKAHLLGY
                     GCFADYATSRRMAGSGANALKFINDLHDKVKPSFLKDMEAVRRYKEEKTGKPVEKLSP
                     WETGYWSEKRRRELYAFDEEDLRPYYSVEKVMEGLFSIYSGLYGITVTPRPTVAFKPG
                     ESGEAPEGAVEVWHPDVLFYELHDAESGEHLGSFYADWHPRDSKRAGAWMNCLSVGEP
                     PHGGKPRAPHLGLMVGNMTKPVGDKPALLSHREVETIFHEFGHLLHQLLSDVEVKSLA
                     GTNVAWDFVELPSQINENWCWERESVDLFAAHYETGEKIPDELFSK"
     assembly_gap    2006..2018
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2019..2457
                     /locus_tag="PK077_08220"
     CDS             <2019..2457
                     /locus_tag="PK077_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HRN24322.1"
                     /translation="RSARNYMSGTDFMRQLCFGKLDLELHVNWPQYKGVPLEETDERI
                     LADYRVPMTDRGPSVARRLTHIFADPTGYASGYYSYKWAEVLEADAFSRFLKEGVLNP
                     QTGRDFRRCILSKGNSKPAAELYRDFMGRDPDAEALLVKSGVL"
     gene            2489..3811
                     /locus_tag="PK077_08225"
     CDS             2489..3811
                     /locus_tag="PK077_08225"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRN24323.1"
                     /translation="MSEQKRLSLAGVGCGSRTRTYMKLAMEQKNRYRIAAAADPVKQR
                     TEAVRDFAPEEERDSVRLFKDAASLLAEPRLADVAIIGTQDDYHYAPCRKAMELGYHV
                     LLEKPIAKTLKEALELRDMARALKRRVAVCHVLRYTQFYRTLKKIIDSGEIGDVITFN
                     ANEGVGAWHFAHSFVRGHWGNSKTSTPMIVAKCCHDMDILYWLMGRRKCLSVASFGEL
                     TFFTKKTLSSPRPERCTEWTTPVGEDPWDARKYATDDECKRWLGMVYDRAQEATAEEI
                     CEWLKTSPWGRDYLQCDNDQPDHQVSIMRFEGGLTGTFTMTAFEQGRHIEVYGTKGKI
                     RAGVFYKENGPGEITVTPHFGGKTRVVELEELAGGYQGHGGGDWGLIEALYDDMLTVP
                     SPEDMTTSIEESAHSHVMAFATEHARLSGQVVDVADFERKVAEGILNY"
BASE COUNT          879 a         1153 c         1170 g          743 t
ORIGIN      
        1 gtggaagcgg tggaagcggt ggaagcggtg gaagcggttc tggcgcccgg ttttggactg
       61 gaaacggcat ttccccgcat cgcgctggaa catgacgtcc aatcccgggg caggaagctt
      121 gttcatcttg aaaaataagc ctgcggtgat aaaaaagggg cgtgaagccg tgcccggaag
      181 ggcggattcc gtccccgccg cattcatcaa gatgcccagc aggccgcggc cgttccgccg
      241 gatgtctctg aaacatggta agttttattt tttcatggct gttgcggcaa atgccggaac
      301 gcggccattt tcattttcaa taacatcata catcaagcaa tatgaaccat ccctatctag
      361 accccgcttt tctggtttcc tggtcccggc ttacgccgga ggccatcagg ccggacatca
      421 cggaagccat ctcccgcgcc aaagagaata tccgggccat ctgcgaccag ccgctggatt
      481 ccctgactta tgaaagcacc ttcggcgctc tggaaaaggc ttccgaggac ctgcacctgg
      541 gctggggccg catcatgcac ctggactccg tcaatgacga acccgcccag cgggaggcca
      601 tcggggaaat gctgccggaa gtggtgacct tctcctcctc cgtgcctctg aacccgcgcc
      661 tgtggacggt gctgaaagcc gcggcctcct gtgactgggt ggaagacctt tcccccgtca
      721 ggcagcgttt catccaggaa acgctggcag acttccgtga gagcggggcg gacctgccgg
      781 acgacgtgaa gccggaatac gcggaaatag aagcccagct ctccctgaag accaagaaat
      841 tcgcggaaaa cgtgctggac tccaccaacg cctgggaact cattgtggaa gatgaggcgg
      901 aactctccgg gctgccggat tccgtgaagg aggccgcccg cctggacgcc ctggccaacg
      961 gctacggcac ggaagaagag ccccgctggc gcttcaccca gaaattcacc tccctccagc
     1021 ctgtcatgca gtttgcggac tcggacagtc tgcgccgccg catgtgggag ggctcctgtt
     1081 ccatcggaaa agggggggaa tacgataatg aagccctcat cgctgaaatc ctggaactga
     1141 gggacaggaa agcccatttg ctggggtacg gctgctttgc ggattacgcc acctcccgcc
     1201 gcatggccgg gagcggggcc aacgccctga aattcatcaa tgacctgcat gacaaggtaa
     1261 agccctcttt cctgaaggac atggaagccg tccgcagata caaggaggaa aaaaccggaa
     1321 aacccgtgga aaaactttcc ccgtgggaaa ccggctactg gtctgaaaaa cgccgccgcg
     1381 aactgtacgc ttttgacgag gaagacctgc gcccgtatta ctccgtggaa aaagtcatgg
     1441 aaggactctt ttccatctac tccggcctgt acggcattac ggtgacgccg cgccctacgg
     1501 tggcgttcaa acccggtgaa tccggggaag cgccggaagg cgcggtggag gtatggcatc
     1561 cggacgtcct gttctatgaa ttgcatgatg cggaaagcgg ggagcacctg ggttcctttt
     1621 acgcggactg gcatccgcgg gactccaagc gcgccggggc atggatgaac tgcctgagcg
     1681 taggggaacc gccgcacggc ggcaaacccc gcgcgcccca tctgggtctc atggtcggaa
     1741 acatgaccaa gcccgtgggg gacaagcccg cgctgctgtc ccaccgggag gtggagacca
     1801 ttttccatga attcggccat ctgctgcacc agctcctttc cgacgtggaa gtgaagtccc
     1861 tggcgggaac caacgtggcc tgggattttg tggaactgcc ctcccagatt aatgaaaact
     1921 ggtgttggga gagggaatcc gtggacctct tcgccgccca ctatgaaacg ggtgaaaaaa
     1981 taccggacga actgttctcc aaaatnnnnn nnnnnnnngc gctccgcccg caattatatg
     2041 agcggcacgg atttcatgcg ccagctctgc ttcggcaagc tggatttgga gcttcacgtc
     2101 aactggccgc agtacaaggg cgttccgctg gaagaaacgg atgaacgcat cctggcggac
     2161 taccgggtgc cgatgacgga ccgcggccct tccgtggcgc gccgcctgac gcacatcttc
     2221 gcggatccca cgggctatgc ctccggctac tactcctaca aatgggcgga ggtgctggaa
     2281 gcggacgctt tcagccgctt cctgaaggaa ggagtgctga acccgcaaac cgggcgcgat
     2341 ttccgccgct gcatcctcag caagggcaac agcaagcccg ccgcggaact ctaccgcgac
     2401 ttcatgggcc gtgatccgga cgcggaagcg ctgctggtca aatccggcgt tctttaacct
     2461 ctttacccac attatctgaa caatccttat gtctgaacaa aagagactct ctctcgccgg
     2521 cgtcggttgc ggttcccgta cgcgcactta catgaagctg gccatggaac aaaaaaaccg
     2581 ctaccgcatt gccgccgcgg cggatcccgt caaacagcgc acggaagccg ttcgcgactt
     2641 tgccccggag gaggaacggg attccgtccg cctgttcaag gatgccgcct ccctgctggc
     2701 ggagccgcgc ctggcggacg tggccatcat cggcacacag gacgactacc attacgctcc
     2761 atgcaggaag gccatggagc tgggctatca tgtcctgctg gaaaaaccca ttgccaagac
     2821 gctgaaagaa gcgctggaac ttagggatat ggcccgtgcg ctgaagcgcc gcgtggccgt
     2881 gtgccacgtg ctgcgctaca cccagtttta ccggaccctg aaaaaaatca tcgacagtgg
     2941 ggaaatcggg gacgtcatca ccttcaacgc caatgaaggc gtgggggcgt ggcacttcgc
     3001 ccactccttc gtgcgcggac actggggcaa cagcaagacc tccactccca tgatcgtagc
     3061 caaatgctgc catgacatgg acatccttta ctggctcatg ggccggcgta aatgcctctc
     3121 cgtcgccagt ttcggggagc ttaccttctt tacgaaaaag acgctctcct caccccggcc
     3181 ggaacgctgc acggagtgga ccaccccggt gggggaagac ccctgggacg cccgcaaata
     3241 cgccacggac gacgaatgca aacgctggct gggcatggtg tatgaccggg cgcaggaagc
     3301 taccgcggag gaaatctgcg aatggctgaa aacctccccg tggggcaggg actacctcca
     3361 gtgcgacaat gaccagccgg accatcaggt ttccatcatg cgctttgagg gaggcttgac
     3421 gggcacgttc acgatgacgg ctttcgaaca ggggcgccac attgaagtgt atggcaccaa
     3481 aggcaaaatc cgcgccggag tcttctacaa ggaaaacggc cccggtgaaa taaccgtgac
     3541 gccccacttt ggcggaaaaa cccgcgtggt ggagctggag gaactggccg gaggctacca
     3601 ggggcacggc ggcggcgact ggggattgat tgaggctctg tatgatgaca tgctgacggt
     3661 gccctctccg gaggatatga ccacttccat tgaggaatcc gcccattccc atgtgatggc
     3721 ctttgccacg gaacacgccc ggctgagcgg gcaggtggtg gatgttgctg acttcgaacg
     3781 caaggtcgcg gaaggcatcc tgaattattg atgcggtttt cctccttgag ggaaaggaaa
     3841 aggcgcaggc gtccaaaatg cctgcgcctt tttgtatata aaatactatc ctttttttag
     3901 gcggatttag aatccggtgt caacataaaa gaatcaaaaa tgcagataaa tgaatctg
//
LOCUS       DAOMSU010000201         3950 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2230_length_3950_cov_2.831065, whole genome shotgun sequence.
ACCESSION   DAOMSU010000201 DAOMSU010000000
VERSION     DAOMSU010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2230_length_3950_cov_2.831065"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PK077_08230"
     CDS             <1..482
                     /locus_tag="PK077_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24324.1"
                     /translation="VVMEKTRFDARETFPDFKLVFTNKGEKTVRLFDDFYPLKDHSPN
                     IHIEIWRKEGEKRIGCIGSVISDGKKPLAWYSPLYTVERLPFSIRFITLQPGEKHEVP
                     IQEAYLLLTYLEHLRNNKRYELEVRFKDGFANPGVRREYVGKADFETSSRSPEGALP"
     gene            677..1282
                     /locus_tag="PK077_08235"
     CDS             677..1282
                     /locus_tag="PK077_08235"
                     /inference="COORDINATES: protein motif:HMM:NF014025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HRN24325.1"
                     /translation="MQDFLSTVILLFIVIDPVGLAPMIQGMLKKYSPAQQKAILTREL
                     VFALSLLLLFFFSGKFLLNLLGLEPSTLSISGGILLFLVALGMVFPAKDMLSSSGRAD
                     GKDEPFIVPIAMPLMAGPSSLSIIMLHASQTPDSISQMTYAGAIVTAWFLSGMVLFIA
                     QKFLRLLGEKGTIALERMMGMVLIMISVQMFMNGLAGYGVK"
     gene            1299..2003
                     /locus_tag="PK077_08240"
     CDS             1299..2003
                     /locus_tag="PK077_08240"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRN24326.1"
                     /translation="MLWLLHGNLGSPADWKPVMEFLRAGGVEARALNLWRYLECCPKS
                     LKDMGRVLCSEIASQDRHPLLCGYSLGGRLAMQAVLAHPPLWKGAIFVSANPGLEHEE
                     ERAARRAKDAEWAVQCLSASWESFLKEWDAQGVFKGSPPPPDRSSLKPWRKSISRAFI
                     DWSVGAQENLAPLLKQSPVPQLWIAGQRDARFSALARRMAGGQAVIIPHAGHRLPLET
                     PERLAECLQPFILQNL"
     gene            2000..2320
                     /locus_tag="PK077_08245"
     CDS             2000..2320
                     /locus_tag="PK077_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HRN24327.1"
                     /translation="MNDPEIPDAEDLRKLVEEIAQTHIPFGMYGPAKYPPKGCPLMDV
                     PQEYLAWFQAKGFPKGKLGRLMEQCLLLKGNGLDSLFDPFRKANGGRTKKNARRRVWD
                     FENE"
     gene            complement(2356..2709)
                     /locus_tag="PK077_08250"
     CDS             complement(2356..2709)
                     /locus_tag="PK077_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24328.1"
                     /translation="MKKLLITLSAVLCMVLPSLSPAEMRQREPRQLAAAWQEVKKQMA
                     DDGKTIRVRDGIQALELLAELGISSVPADGVRLSRGSLTIVVTRGKKEDGVFYVLAVK
                     EEGGVSLEVKMVPSS"
     gene            complement(2706..2936)
                     /locus_tag="PK077_08255"
     CDS             complement(2706..2936)
                     /locus_tag="PK077_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24329.1"
                     /translation="MDQKTTYSYQRTPGLDCPKCGVYFPTTIPDLLSGSIRCPYCGLT
                     LYIDRKESGHAMQALENFQNALDKQLPSASLS"
     gene            complement(2951..3547)
                     /locus_tag="PK077_08260"
     CDS             complement(2951..3547)
                     /locus_tag="PK077_08260"
                     /inference="COORDINATES: protein motif:HMM:NF023085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2589 domain-containing protein"
                     /protein_id="HRN24330.1"
                     /translation="MMSDSTNNNKVNGHIMSLQQLISAPLVATIDADAMSTERYMKHF
                     MSLAFESYNPVDGSTGALRLISFNFTDSDASGGAEKKVSVPLVSLVTLPLLQIKQADF
                     DFDINIIDAVAAAPNEHFSLNRGEVESPRGKERDEGLNFRASLAPQSGRGASSSLQAN
                     MKIHVSMQQADMPSGLSQFLQLASRPHYEDVPSREKND"
     gene            complement(3554..>3950)
                     /locus_tag="PK077_08265"
     CDS             complement(3554..>3950)
                     /locus_tag="PK077_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24331.1"
                     /translation="AAVSAHEGALLKAEPGLDRGLLLELLAGVAEREFMDNPELEDLF
                     QGTRGDPLRQECRANLREGLSSFLAALAEKYVLTRQESSVVVDVEVEADKLKKMPEEA
                     VQTLRLNISPVSIPSVLAEQNDVSGLSAQ"
BASE COUNT          864 a         1134 c         1068 g          884 t
ORIGIN      
        1 ccgtggtgat ggaaaagacc cggtttgacg caagagagac gttccctgac ttcaagctgg
       61 tgttcacgaa caagggcgaa aagaccgtgc gcctgttcga cgacttttat cccctcaagg
      121 atcatagccc taatatccac atcgaaatat ggcgaaagga aggcgagaaa agaattgggt
      181 gtatagggag cgtaataagc gatggaaaga agcctttagc ttggtatagt cccctgtata
      241 cagttgaacg tctaccgttt tccatacgtt tcattacgtt gcagccggga gaaaagcatg
      301 aagttcccat ccaggaagct tatttgttgc tgacgtattt agagcacctt cgcaataaca
      361 agaggtacga gcttgaagtc agattcaagg atggatttgc gaaccccggt gttcgaaggg
      421 agtatgtagg gaaagcagat tttgaaacat cgagccggtc tcccgaagga gccttgcctt
      481 aatagctttt ctgttttatt aaaaagctat ggtctcctac gcaaagtttc cccagaattc
      541 cttaaatcgc tttccaggca taaaggcccg gctcctgcgc tggtgaagaa aatagcaatc
      601 cgggcagaga aaatttccac tgctggcggt tccctttgac gtacacgccc gtatcgttta
      661 caacggggca tatcccatgc aggacttcct ttccactgtt attcttctgt tcatcgtcat
      721 cgacccggtg ggtctggcgc ccatgatcca gggaatgctg aaaaaatact cgcccgccca
      781 gcagaaggct attctgacac gggagcttgt atttgcgttg agcctgctgc tccttttctt
      841 cttttccgga aaattcctgc tgaacctgct ggggctggaa ccttccaccc tgagcatttc
      901 cggaggcatc ctgctgtttc tcgtagcgct gggcatggtc ttccccgcca aggatatgct
      961 ttcttcttcc ggacgagcgg acggaaaaga cgaacccttc attgttccca tcgccatgcc
     1021 cctgatggcc ggcccctctt ccctgtccat cattatgctg cacgcctccc agactccgga
     1081 cagcatttcc cagatgacct atgccggggc catcgtgacc gcctggttcc tctccggaat
     1141 ggtgcttttc atcgcccaga agttcctgcg tctgctggga gaaaaaggga caatcgccct
     1201 ggaacggatg atgggcatgg tgctcatcat gatttccgtc cagatgttta tgaacggcct
     1261 cgccggctac ggcgtcaaat agaaaggcgg ttccgaccat gctttggctt ctgcacggca
     1321 atctgggttc tccggcggat tggaaacccg tcatggaatt tcttcgtgcc ggaggagtgg
     1381 aggctcgcgc cctgaacctc tggaggtatc tggaatgctg cccaaaaagc ctgaaggata
     1441 tgggtcgcgt gctgtgttcc gaaatagcct cccaggatcg ccaccccctc ctctgcggct
     1501 attccctggg aggcaggttg gccatgcagg ccgttttggc ccacccccct ctctggaaag
     1561 gagccatatt cgtcagcgcc aatcccggcc tggaacatga agaggaacgg gcggcgcggc
     1621 gggcgaagga tgcggaatgg gccgtccaat gcctttccgc ttcctgggaa agctttttga
     1681 aggaatggga tgcccagggc gtatttaaag gcagtccgcc tcctccggac cgttcttccc
     1741 tgaaaccgtg gcgcaaatcc atctcccgcg cctttattga ctggtctgtg ggcgcacagg
     1801 aaaacctggc tccgcttttg aagcaatccc ctgttcccca gctgtggatt gccggacagc
     1861 gcgatgccag attcagcgcc ctggcgcggc gcatggcagg agggcaagcc gtcatcatcc
     1921 ctcatgccgg ccaccggctt cccctggaaa cgccggaaag gctggcggaa tgcctgcaac
     1981 catttattct gcaaaaccta tgaacgatcc tgaaatacca gatgcggaag atttgagaaa
     2041 actggtggag gaaatcgcgc agacccacat ccctttcggc atgtacggtc ctgccaaata
     2101 cccgcccaag ggatgtccgc tgatggacgt cccccaggaa tatctggcct ggttccaggc
     2161 caaagggttc ccgaaaggga aactgggccg tctgatggaa cagtgcctgc tgctgaaagg
     2221 gaacgggctg gactctcttt ttgacccctt ccggaaagcc aacggcgggc ggacgaagaa
     2281 aaacgcccgc cgccgcgtgt gggattttga aaatgaatga ccttcctccg cggcagaaat
     2341 gccccctccg gcaaatcagg aagaaggaac catcttcact tccaggctta cgcccccttc
     2401 ttccttaaca gccagcacgt aaaaaacccc gtcctctttc ttcccacgcg tgaccacaat
     2461 ggtcagactg ccgcgggaca gcctgacgcc gtccgccgga acggaagaga tccccagttc
     2521 cgccaggagt tcaagcgctt gaatgccgtc cctcacacgg atggttttcc cgtcgtctgc
     2581 catctgcttc ttgacttcct gccaggcggc ggcaagctgg cggggttccc gctgccgcat
     2641 ctccgccggg gacaaggacg gcagaaccat gcacagcacg gcggacaggg taatcaacag
     2701 tttcttcatg acagggaggc ggagggaagc tgcttgtcta atgcgttctg gaaattttcc
     2761 agggcctgca tggcatggcc tgattctttc cggtcaatgt aaagcgtcag gccgcaataa
     2821 gggcacctga tggagccgga cagcaagtcc ggaatggtcg tggggaaata caccccgcat
     2881 ttggggcagt caaggcccgg agtacgctgg taggaataag ttgttttctg gtccatatcg
     2941 tttttttcaa ttaatcattc ttttcccggg aagggacatc ctcataatgc ggcctggaag
     3001 ccagctggag aaattgggac aggccggaag gcatgtcagc ctgctgcatg gagacatgga
     3061 ttttcatgtt cgcctgcagg gaggaagaag cccctctccc ggactgcgga gccagggaag
     3121 cccgaaaatt cagcccttca tcccgctctt ttccgcgggg ggactccacc tctcccctgt
     3181 tcagggaaaa atgttcattc ggcgcggcgg ctacggcgtc aatgatattg atgtcaaaat
     3241 caaaatccgc ctgcttgatt tgcagcagcg gcagcgtcac caggctgacg agcggcacgc
     3301 tgaccttctt ttccgcgccg ccggaagcgt cgctgtccgt aaaattgaag ctgatcaacc
     3361 tcaacgcccc ggtggaaccg tccacaggat tgtaggactc aaaggccagg gacatgaaat
     3421 gcttcatgta gcgttccgtg gacatcgcat ccgcgtcaat cgttgcgacc agcggcgctg
     3481 aaatcagctg ctgcaggctc ataatgtgcc cgttcacctt gttattattt gtagaatcac
     3541 tcatcatcta tcactattgc gcggacaacc cggaaacatc attctgctcc gccagaacgg
     3601 agggaatgga aacaggggat atattcaggc gcagggtctg cacggcttcc tccggcatct
     3661 ttttcagctt gtccgcctct acttccacat ccaccaccac actgctttcc tgcctggtga
     3721 ggacgtactt ttccgccaga gcggccaaga aagaggaaag gccttcccgg agattggcgc
     3781 ggcattcctg cctgaggggg tctccccggg ttccctggaa caggtcctcc agctccggat
     3841 tatccatgaa ttccctttcc gcgacgcctg ccagaagctc cagcagcaat cccctgtcca
     3901 gcccgggttc cgccttcagg agcgcgccct catgagcgga aacggctgcg
//
LOCUS       DAOMSU010000202         3854 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2302_length_3854_cov_2.459595, whole genome shotgun sequence.
ACCESSION   DAOMSU010000202 DAOMSU010000000
VERSION     DAOMSU010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3854)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3854
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2302_length_3854_cov_2.459595"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PK077_08270"
     CDS             complement(<1..856)
                     /locus_tag="PK077_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24332.1"
                     /translation="MLDFLGVTEHFSCMENTPFSFPALFRDMAGEAVSLDGVIFSGSI
                     VSANQEMVEISIEKGESSNEVIFSFPALPEGRWSYNVLVQADDGSQRILFSGYISVLG
                     VSRVAQLAGGPPMKNRTLLVAMPGEATMRLRMEWMATTAAQAFAYHALQASKNAHADA
                     ETASQAAKTATDAAATAAARAEEAEGYAGAAWASKRAAADSATAAGTSATNAARDAKS
                     ANDAKTSVESLAATWPETVSNGKQQIIEAKNEAVTAIQDKQADSVLAVGRASQTAQQN
                     IAGARTDAV"
     gene            complement(979..1971)
                     /locus_tag="PK077_08275"
     CDS             complement(979..1971)
                     /locus_tag="PK077_08275"
                     /inference="COORDINATES: protein motif:HMM:NF040375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HRN24333.1"
                     /translation="MEFNLSIADMLRTKYSTIFEREIQQVTSILEPYCSVLPGRGKDM
                     EIPYVGKTEFKEIGNRFVEASPHELSMGKRVIKPQRYADSLHKDDIDNILLNDLELSI
                     SDFIAEMKKAGKRLLDQVLLGVVPDTDNPGKFRIRTTSDSVCGGMLAPNYTGNSGATL
                     TNLDPSLVVPADFKMDGTKNPAGFLLDQIVEAKRMLEENYAWDEASGDTLCLAISSTM
                     KAQMIMWEEQKNKNYGFSVLEHGKVNPMLNVRFLVTNMLPFDEDGNRICPMWVKSRLV
                     LSPWDQMKFSIVRPDKYQNLSVVRADAACMYGASRKDEKSFVQILCKEKATAGA"
     gene            complement(2052..3158)
                     /locus_tag="PK077_08280"
     CDS             complement(2052..3158)
                     /locus_tag="PK077_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRN24334.1"
                     /translation="MLGSQEWQEMGMRQYFEPTDYAHLYREKNSGIYYARVDSRQGGR
                     KTIRRSLKTKDINEAIAKMAAFLQEMGTNTPTIGNISWYVAVDTYIAQQKMRPNLKPR
                     ALESALLFAEHARKLVEQDVAAEAITANMCRLWWTKKAQSCSPRTANGALGAVRKIFA
                     MLRDSGYVVSDPTAKLERLSLKPTEFFIPGKEEFVRIVEEIRRAPLLRKYREKSLDSP
                     AADMVAFLAYSGLRIEEARRLVWGDIGKDSISVPAIKHAVKRRILYINPALRNVIERM
                     KKTNNNLTATSPVFVIENPRKALTNACVRLGLPHVRVHDLRHFFATTCIEQGVDIPTV
                     AKWLGHQDGGALAMRVYGHLRDEHSKEQASRLRF"
     gene            complement(3259..3335)
                     /locus_tag="PK077_08285"
     tRNA            complement(3259..3335)
                     /locus_tag="PK077_08285"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3299..3301),aa:Pro,seq:ggg)
     gene            3438..>3854
                     /locus_tag="PK077_08290"
     CDS             3438..>3854
                     /locus_tag="PK077_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24335.1"
                     /translation="MKQVQVAVVGASGYTGQELLRILLNHRGVKLVCATSRQYAGQPL
                     WEVFPRFRQVPGSGLKFTDSDVEAIAATGAEVAFLALPHGVAASYARGLVDRGVRVID
                     LSADFRLDSPEVDEIDTTENQAQEALIFLNTISVIFV"
BASE COUNT          930 a         1065 c          932 g          927 t
ORIGIN      
        1 caacggcatc cgttcgtgcg ccggctatat tctgctgcgc ggtctgtgag gcacgaccta
       61 cggcaagcac ggaatcggct tgcttgtcct gaatagcagt aacagcctca ttcttggcct
      121 caataatctg ttgctttcca ttgctgaccg tttccggcca ggtggcggcc agcgactcca
      181 cagacgtttt agcgtcattg gcgctcttgg cgtcacgagc cgcgttagtt gcggatgtgc
      241 cggcggcggt cgcagaatcg gcggcagccc ttttggaggc ccaggcagcc cctgcatagc
      301 cttccgcctc ttcggcccgt gcagcagcgg tggctgccgc gtccgttgcc gtcttggctg
      361 cctggctcgc cgtttctgcg tccgcatgag cattcttgga agcctggagc gcatgatagg
      421 caaaagcctg tgcagcagta gtagccatcc actccatacg gagacgcatt gtcgcttccc
      481 caggcatagc gacaagcagc gtccggttct tcattggcgg accgcctgcc aactgtgcga
      541 cacgagaaac accgagtacg gaaatatacc cggaaaacaa aattctttgg gaaccatcat
      601 ctgcctgaac caggacattg taagaccatc ttccttccgg caaggcgggg aatgaaaaaa
      661 tcacctcatt agaagactcc cccttctcaa tggaaatctc taccatctcc tgattggcgg
      721 atacaatgct ccctgaaaaa atcacgccgt ccagagaaac ggcttcccct gccatatccc
      781 gaaacagggc agggaaggaa aacggagtat tctccatgca ggaaaaatgt tccgtgacgc
      841 ccaggaaatc taacatgcct ccctcttagc acacattttt gccgcacgaa tacaaccgta
      901 acttgaacaa gcaaaaaaat ccgctccctg ccgaaacagg gagcggaaca aacaacaacg
      961 aataccctgg aaagataatt aagctccagc cgtcgccttc tccttacaaa gaatctgcac
     1021 gaaagatttt tcgtctttcc tggaagcccc atacatgcaa gcggcatctg ctcgaacaac
     1081 agaaaggttc tgatacttgt ccggtcgcac gatagaaaac ttcatctgat cccacggaga
     1141 aagaaccagg cggctcttga cccacatcgg acaaatacga ttgccgtcct catcaaacgg
     1201 aagcatattc gtgaccagga atcggacatt cagcattgga ttcaccttgc catgttccag
     1261 cacggaaaaa ccgtaattct tattcttctg ctcctcccac atgatcatct gcgccttcat
     1321 cgttgaggaa atcgccagac aaagagtgtc gccggaagcc tcgtcccatg cgtaattctc
     1381 ttccagcatg cgcttggctt cgacaatctg gtccagcagg aacccggcag gattcttcgt
     1441 cccatccatc ttgaaatcag caggaacaac cagacttgga tcaagattgg tcaacgtggc
     1501 gcctgaattg cccgtgtaat taggtgcaag cattcccccg caaacactat ccgaagttgt
     1561 acggatgcgg aactttccgg gattatccgt atcgggaacc accccaagca aaacctgatc
     1621 aagcagcctc ttgccggcct tcttcatttc cgcgataaaa tcgctgatac tgagttcaag
     1681 gtcgttcaat aggatgttgt caatatcatc cttgtgaaga gagtccgcat aacgttgagg
     1741 tttaattaca cgcttcccca tggaaagctc gtgcgggctg gcctctacga atctgttgcc
     1801 gatctccttg aactccgtct tgcccacata gggaatctcc atatctttcc cgcgaccggg
     1861 gagaacggaa caatacggct caagaataga cgtaacctgt tgaatctcac gctcgaaaat
     1921 tgtcgaatac ttcgtgcgga gcatatctgc aatagaaaga ttaaactcca tgcccctgtc
     1981 attgcacgga acgtgatcgg agacatacaa ccacgacttg aacaaaaacg cccacgactt
     2041 gaacaaaaca atcaaaagcg cagcctggaa gcctgttcct tgctgtgctc gtcgcgcaaa
     2101 tgaccataca cccgcatggc gagagcgccc ccgtcctggt gccccagcca cttggcgacg
     2161 gtcggaatat ctactccctg ctcaatacac gtcgtggcga aaaaatggcg caaatcatgc
     2221 acccgaacat ggggcaaccc caggcggaca caggcatttg taagagcttt ccttgggttt
     2281 tcaataacga acacaggaga tgttgcggtc aagttattgt ttgttttttt catccgctca
     2341 atcacattcc gtagagctgg gttaatatac aatatccgtc ttttgactgc atgtttaatt
     2401 gccgggacgg atatggagtc ttttccaata tcgccccata ccaagcgtcg ggcttcttca
     2461 atccgcagac cagagtaggc caaaaaagcc accatatctg ctgcagggga atcaaggctc
     2521 ttttcccggt actttcgcag taaaggagca cgcctgattt cttctacaat acgaacaaat
     2581 tcttcttttc ccgggatgaa aaattccgtc ggtttcagac tcaatctctc cagttttgcg
     2641 gtagggtctg aaactacgta cccggaatcc ctgagcatag caaatatttt tcgaactgca
     2701 ccaagagctc cattggccgt tcttggcgaa caactctgtg cttttttggt ccaccataac
     2761 cggcacatat tggccgtgat ggcttccgct gccacatctt gttcaaccag cttgcgagca
     2821 tgctccgcga aaagcagagc agattcaagg gccctaggtt taagattggg cctcatcttt
     2881 tgctgggcaa tgtaggtatc taccgctaca taccaagaga tgtttccgat agtcggggta
     2941 tttgtcccca tctcctgcaa aaaggcagcc attttggcaa tagcctcatt tatatccttt
     3001 gttttaagtg aacgtcttat cgtttttcgg ccaccttgtc tggaatccac acgagcatag
     3061 taaatgccag aattcttttc ccggtacaag tgggcgtaat cggtaggctc gaagtattga
     3121 cgcataccca tctcttgcca ttcctgtgac cccaacacaa cattttttca ttttgacgtg
     3181 tggcctgacg tgtggctaat ctaaaaacag gggaaaacaa aaaccgctgt aagcgtttca
     3241 gcttacagcg gttaaaaatg gtcgggttga caggattcga acctgcgacc ccctgcaccc
     3301 catgcaggtg cgctaccaga ctgcgctaca acccgatgaa ctcggaccga ccggggtcgt
     3361 gtctcatgac ggcgaggtgt atataggaaa actttagtgt tggcaagagt cttttttcat
     3421 agcatacctt ttttgtcatg aagcaagttc aagttgcagt cgtcggggcc agtggctaca
     3481 ccgggcagga attgttgcgc atcctgctga accatcgcgg ggtgaagctg gtgtgcgcta
     3541 cttcccgcca gtatgccggg cagccgttgt gggaggtttt tccccgtttc cgtcaggttc
     3601 cgggttccgg tctgaagttt acggattccg atgtggaggc gattgccgct actggggcgg
     3661 aggtcgcttt tctggccctg cctcatggcg tggctgcttc ctatgcccgc ggcctggtgg
     3721 acaggggcgt gcgcgtgatt gacctgagcg cggatttccg tctggacagc ccggaggtgg
     3781 acgagatcga caccacggaa aaccaggccc aggaagccct gattttcctg aacaccatct
     3841 cggtgatttt cgtg
//
LOCUS       DAOMSU010000203         3822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2326_length_3822_cov_2.286700, whole genome shotgun sequence.
ACCESSION   DAOMSU010000203 DAOMSU010000000
VERSION     DAOMSU010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2326_length_3822_cov_2.286700"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..666)
                     /locus_tag="PK077_08295"
     CDS             complement(<1..666)
                     /locus_tag="PK077_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HRN24336.1"
                     /translation="MQHTDQIQALSEFLLEYATTLMGAGVHTNRAVRNISRIAAAYGY
                     SADMTIFQRNITMSLICKDDETLRRTSVRKLKPLAFNLNLIQQLSELSWLPVDNNVSI
                     AEMEQAFRSMVRTKRFSRWTDLLLVSVGNAAFCRLFNGDLWAMLTVFAATMLGFLAKQ
                     QLTRLKYNPLGVIILSAFTASMAAACAVLFQIGSTPQIALAASVLFLVPGVQMINSIM
                     DLMH"
     gene            complement(684..812)
                     /locus_tag="PK077_08300"
     CDS             complement(684..812)
                     /locus_tag="PK077_08300"
                     /inference="COORDINATES: protein motif:HMM:NF014961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRN24337.1"
                     /translation="MQPVSSVCALVFSHPESAYFSVGVTGEDQQEDYASRKRASRP"
     assembly_gap    855..864
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<865..>3822)
                     /gene="metH"
                     /locus_tag="PK077_08305"
     CDS             complement(<865..>3822)
                     /gene="metH"
                     /locus_tag="PK077_08305"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HRN24338.1"
                     /translation="VETIFDTLNAKAALFAIEEIFEEQPESSVPVMVSVTLTDKAGRT
                     LSGQTIEAFWNSIRHVRPFSVGINCALGPDLMRPFAEELSGLADCHMSIYANAGLPNP
                     LSPTGYDLLPADMARFMKEYAEQGLLNIVGGCCGTTPEHIGAIAAAVEGMPPRVPAPQ
                     TPALRLSGYEAYNHTREKNTLFVGERCNVAGSPKFARLIREGNYEEAVSIARQQVENG
                     ALVLDFCFDDGLIDGPQAMVRFLNLVSAEPDIARVPFMVDSSKWEVLEAGLQCMQGKG
                     IVNSISLKEGEEEFLKKAALIKKYGAAVVVMAFDEQGQAANYEDRVRISRRAYDLLVN
                     RVQFPPEDIIFDPNVLTVGTGIAEHADYALDFFKAAGWISRNLPHAHISGGISNVSFA
                     FRGNNPVREAMHSAFLYHATQQGLDMCIVNAGMLEVYDNIPKDRLELIEDVLLNRRTD
                     ATERLTDYAEKLAAEKTGDGKEKKTVLAWREQDVAKRLEYSLIKGITEFVDADTEEAF
                     QELGSPLNVIEGPLMAGMKVVGQLFGDGKMFLPQVVKSARVMKQAVAWLTPYIEADSK
                     GAAKAGKAVIATVKGDVHDIGKNIVGVVLGCNGFEMIDLGVMVHCDTILDRAEAEQAD
                     MVMLSGLITPSLEEMSHVAAEMERRGMTIPLMVGGATTSALHTALKIAPHYQGAVVHT
                     EDASQVVPAAASLVGEKKDSYIAAVKAQQEELRNNHENKPVRDLLPLEKARELRWKGA
                     EGGYLPPVPARLGPVSIGSLHSSVSCGCCSDDPRYYVTIQELIERMDWTPFFHAWELH
                     GTWDRARREFRTKDPSKAKAAAALYQDALRLLEQAVKENRYQARGVIGIFPANSTASH
                     DDITVWADESRTIPLATLLTQRQQLDKQGKPRLALADFIAPEGVRDYVGAMAVSIHGS
                     RRWAKEWEARNDSYRALLVSSLADRLVEAFAAIAHDKLRLLWNIPEGSGVRPACGYPS
                     QPDHQEKETVFTL"
BASE COUNT          738 a         1125 c         1145 g          804 t
ORIGIN      
        1 gtgcatcagg tccataatgg agttaatcat ctggacgccg ggcaccagga acagaacgct
       61 ggctgccagg gcaatctgcg gtgtggagcc tatctggaaa aggacggcgc acgcggccgc
      121 catggaggcg gtgaaggcgg aaaggatgat gacccccagc ggattgtatt taagccgggt
      181 aagttgctgc ttggccagaa agcccagcat ggtggcggcg aacacggtca gcatggccca
      241 caggtctccg ttaaacaggc ggcagaaggc ggcgttgccc acgctgacca ggagcagatc
      301 tgtccagcgg gaaaatcttt tggtgcgcac catggagcgg aatgcctgtt ccatttccgc
      361 aatgctgacg ttgttgtcca ccgggagcca gctcaattca ctgagctgct ggatcaggtt
      421 caggttgaac gccaggggtt tcagtttccg gacggaggtg cggcgcagcg tctcgtcatc
      481 cttgcagatc aggctcatgg tgatattgcg ctggaaaatg gtcatgtccg cgctgtatcc
      541 gtacgcggcg gcaatgcggg aaatgttgcg cacggcgcgg ttggtgtgca cccccgcgcc
      601 catgagggtg gtggcgtatt ccagcaggaa ttccgaaagg gcctgtatct ggtcggtatg
      661 ctgcatcaaa gggaaagggg aggtcagggc cgggaggccc gctttctgga ggcgtaatcc
      721 tcctgctggt cttccccggt gacgcccacg gagaaataag cgctttccgg atgggagaag
      781 acgagggcgc agacggaaga aacgggctgc atcatccatg tttccgtcag gctcatgccc
      841 gcttcctccc gggcnnnnnn nnnngcaggg tgaagacggt ttccttttcc tggtggtccg
      901 gctggctggg gtacccgcag gcgggacgca cgccggaccc ttccggaata ttccacaaaa
      961 ggcgcagctt gtcatgggcg atggcagcga aagcctccac caggcggtcc gccagagagc
     1021 tgaccagcag ggcgcggtag gagtcgtttc tggcttccca ttcctttgcc cagcggcggg
     1081 agccgtggat gctgaccgcc atggccccta cgtaatccct gacgccttcg ggagcgatga
     1141 agtccgccag cgccagccgg ggtttcccct gtttgtccag ttgctggcgc tgcgtcagca
     1201 gcgtagccag gggaatggtt ctggattcat ccgcccagac ggtgatgtcg tcatggctgg
     1261 ccgttgaatt ggccgggaaa atgcctatga cgccgcgcgc ctggtagcgg ttttccttca
     1321 cggcctgttc cagcaggcgg agggcgtcct ggtacagagc cgcagccgcc ttagccttgg
     1381 acggatcctt ggtgcggaac tcccggcggg cgcggtccca tgtgccgtgc agttcccagg
     1441 catggaaaaa aggcgtccag tccatgcgtt cgataagctc ctgaatagtt acgtaataac
     1501 gcggatcgtc actgcagcag ccgcagctta cggagctgtg caggctgccg atggaaaccg
     1561 ggcccagacg ggcgggaacg ggggggagat agccgccttc cgcccccttc caccgcaatt
     1621 ccctggcttt ctccaggggc aggaggtctc ggactggttt gttttcatga ttgttgcgca
     1681 attcctcctg ctgggctttc acggcggcaa tgtaggaatc ctttttttct cccaccaggg
     1741 aggcggctgc gggaaccacc tgggaggcat cctccgtatg caccacggct ccttgataat
     1801 ggggggctat tttcagggcc gtgtgcaggg cagatgtcgt agctcccccc accatcaggg
     1861 gaatggtcat cccccggcgt tccatctccg cggctacatg ggacatctct tccaaggaag
     1921 gggtgatcag cccggaaagc atcaccatat ccgcctgttc cgcttccgcc cggtccagaa
     1981 tggtatcgca atggaccatg acgcccaggt caatcatttc aaaaccattg cagcccaaca
     2041 ccacgccgac gatattcttg ccgatgtcat gcacgtcccc cttcacggtg gcgatcacgg
     2101 cttttcccgc cttggcggca cctttgctgt ccgcttctat atagggggtg agccaggcta
     2161 cagcctgttt catgacgcgg gcgcttttta ccacctgcgg aaggaacatt ttgccgtccc
     2221 caaacagttg ccccacaacc ttcatgccgg ccatcagagg gccttcaatc acgttcagcg
     2281 gggaccccag ctcctgaaaa gcctcctccg tatccgcgtc aacaaattcg gtaatgccct
     2341 tgataagaga atattccagg cgtttggcaa cgtcctgttc cctccaggcc agtacggttt
     2401 ttttctcctt tccgtctccg gttttttccg cagccagttt ttccgcgtag tccgtcaggc
     2461 gttccgtggc gtccgtcctc cggttcagca gaacgtcttc aatcagctcc agcctgtcct
     2521 tgggaatatt gtcgtacacc tccagcatcc cggcgttcac gatgcacatg tccagcccct
     2581 gctgcgtagc gtggtacagg aaagcggaat gcatggcttc ccgcaccggg ttgttgccgc
     2641 ggaaagcaaa ggagacattg gaaatgccgc cggaaatgtg ggcatgcggc aggttccggg
     2701 aaatccagcc tgcggccttg aagaaatcaa gagcgtaatc ggcgtgttcc gcaatgccgg
     2761 tgcctacggt caggacgttc gggtcaaaaa tgatgtcttc cggaggaaac tgcacccggt
     2821 ttaccagcaa gtcataggcc cgtctggaga tgcggacgcg gtcctcgtaa ttggcggcct
     2881 gcccctgttc gtcaaacgcc atgaccacga ccgccgcgcc gtacttttta atcagcgctg
     2941 ctttcttcag gaattcttct tccccctcct tcagagaaat ggaattcacg attcccttgc
     3001 cctgcatgca ttggagcccc gcttccagca cctcccactt ggaggaatcc accatgaaag
     3061 gaacgcgggc gatatccggc tctgcggaaa caaggttcag gaagcggacc atggcctgcg
     3121 gcccgtcgat caggccgtcg tcaaaacaga aatccaatac cagcgcgccg ttttccacct
     3181 gctgccgggc aatggatacc gcttcctcgt aatttccttc acgaatcagg cgggcgaatt
     3241 tgggggaacc cgccacattg cagcgttccc cgacaaagag ggtgtttttt tcccgcgtat
     3301 ggttgtacgc ttcatatccg gacaggcgaa gcgcgggcgt ttggggagcc ggaacgcgcg
     3361 gaggcattcc ttccacggca gccgcaatgg cgccgatatg ctccggagtg gttccgcagc
     3421 agccgcccac gatgttcagc aggccctgtt ccgcgtattc cttcatgaat cgggccatat
     3481 ccgccggcag aaggtcgtag ccggtagggc taagcgggtt gggaagcccg gcattcgcat
     3541 aaatggacat gtggcaatcc gccagtccgg acagttcttc cgcaaacggg cgcatcaggt
     3601 ccggccccag ggcgcaatta atgcctacgg agaaggggcg cacgtggcgg atggagttcc
     3661 agaatgcttc aatggtctgg ccggacagcg tgcgtccggc tttgtccgtc agcgtgacgg
     3721 acaccatgac cggaacagag ctttccggtt gttcctcaaa aatttcttca atggcgaaca
     3781 gggcagcctt ggcgttcagc gtgtcgaaaa tcgtctccac ca
//
LOCUS       DAOMSU010000204         3783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2362_length_3783_cov_2.994903, whole genome shotgun sequence.
ACCESSION   DAOMSU010000204 DAOMSU010000000
VERSION     DAOMSU010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2362_length_3783_cov_2.994903"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(194..1324)
                     /locus_tag="PK077_08310"
     CDS             complement(194..1324)
                     /locus_tag="PK077_08310"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004859.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HRN24339.1"
                     /translation="MTTEPYGKKNPFPAPVLSVRHITGEGSPKETIHIEYSLDGSGMN
                     YIAGDALAVIPANDPALADALIDKLGLSPDSQVPTPEGETASLKDALVHCYDITNVNK
                     ALLTKWAAASGSQELNTLLAGDKDALSDFLWGRDVLDLATEYPASFESAEAFVGILKK
                     IMPRLYSIASSPNAHPEQVHLCVGAVRYTARDRKRGGVCSTYMADRLQPGHTARVFVH
                     TNKNFRLPEDGDTPIIMVGPGTGIAPFRAFWEERAASGAKGGNWLFFGNPYRATDFCY
                     EDELNQLTGTGKLKLSVAWSRDQEKKVYVQHLMVQEGEELWKWLEAGACFYVCGDASR
                     MAKDVDNALHEVIQTWGHKTPEEAAAYVADMKQHRRYQRDVY"
     gene            complement(1353..2372)
                     /locus_tag="PK077_08315"
     CDS             complement(1353..2372)
                     /locus_tag="PK077_08315"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HRN24340.1"
                     /translation="MAAHSDTPFKRLPVKIIGTGSYVPERRLTNADLEKMVDTSHEWI
                     IERTGIVERRIAAPDEYTSHMGTKAAQRALESCGLKPEDIDLIIVATITPDTFTPSTS
                     CYIQDALGAKNAAAFDISAACSGFLFAMKTAVQYIGTGQMKTALIIAAEKLSTVVNWE
                     DRSTCVLFGDGAGAAVLQASTGEEGEGSVLATDIGSDGSLHDLLQIPGGGSRCPTTAE
                     NAHERLATLAMRGNETFRHAVTRMKASAASVIERAGLTADDIQLIIPHQANLRIINAV
                     AQRLSIPEDKVFVNIEKYGNTSAAAVAIAFDEARRSGRFGKGDNVVLVTFGAGLTWSA
                     AVIRW"
     gene            complement(2494..3513)
                     /gene="ruvB"
                     /locus_tag="PK077_08320"
     CDS             complement(2494..3513)
                     /gene="ruvB"
                     /locus_tag="PK077_08320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRN24341.1"
                     /translation="MGESFYTRTTEAPSSAFDLSLRPPAFSEFCGQEKIKDRLMLMVE
                     AARQRDDVLDHILLSGPPGLGKTTLANIIANAVGCRIHTTSGPQIEKAGDLAGVLTNL
                     EKGDILFIDEIHRLHPAIEEYLYPAMEDFRLDIIIDQGPNARSIQLNLPKFTLVGATT
                     RAGMLTSPLRSRFGLVNRLDYYTREELCAIIERSAGLLNVPVDPEGALQIALRSRGTP
                     RVANSLLRWVRDYAQVRGDGVITEQLAHDALTMIEIDDDGLDEMDKRLLEAMIYKFNG
                     GPVGLSSLAVAVGEDASTLEDVHEPFLIMQGYISRTPRGRVAMPSAYLKMGAAPPANS
                     QGWLL"
BASE COUNT          757 a         1061 c         1200 g          765 t
ORIGIN      
        1 attcagaatg ctcttttgga cgcgggattt tggcatgtta aagctaaatc ggcatattgg
       61 ggaaacaaat ccggtcagga gaaatgatta ctggcgaaag aaaaacaggg aaatatcaaa
      121 aaataaataa aggagcgaca aaaaaactcc ttcgcataat ggaaggagtt agaaggcttg
      181 ggcatgggcc aaatcaatac acgtcccgct ggtaacggcg gtgctgtttc atgtccgcca
      241 catatgccgc ggcttcctcc ggagttttat ggccccaggt ctggatgact tcatgaaggg
      301 cgttgtccac gtccttcgcc atgcgggagg cgtccccgca gacgtagaag caggcgccgg
      361 cttccagcca tttccagagc tcttcgcctt cctggaccat gaggtgctgc acatacacct
      421 tcttttcctg gtcgcgggac caggccacgg acaacttcag tttgccggtg ccggtcaact
      481 ggttcagctc gtcttcataa cagaagtcgg tggccctgta cggattgccg aagaacagcc
      541 agttgccgcc cttggcgccg gaagcggcgc gttcctccca gaatgcccgg aaaggggcga
      601 tgccggtgcc ggggcccacc atgatgatgg gggtgtcccc gtcttccgga aggcggaaat
      661 tcttgttggt atgtacgaat accctggccg tatggcccgg ctggaggcgg tccgccatat
      721 aggtggagca aacgccgccg cgcttgcggt cacgggccgt atagcgcacc gcgcccacgc
      781 acaggtgaac ctgttcggga tgggcgttcg ggctggaggc gatggaataa aggcgcggca
      841 tgatcttttt caggatgccg acgaaagcct ccgcggattc gaaggaagcg gggtattccg
      901 tggccagatc cagcacgtcc cggccccaca ggaaatcgct gagggcgtcc ttatccccgg
      961 ccagaagcgt gttcagctcc tggctgccgg aggcggcggc ccacttggtc agcagggctt
     1021 tgttgacatt ggtaatgtca tagcagtgaa ccagggcgtc tttcaggctt gcggtttccc
     1081 cttcgggcgt gggaacctgg gaatccgggc tgaggccgag cttgtcaatc agagcgtccg
     1141 ccagggccgg atcattggcg ggaataacgg ccagggcgtc cccggcaata tagttcatcc
     1201 cggaaccgtc caggctgtat tcaatgtgga tggtttcctt ggggctgcct tccccggtga
     1261 tgtgcctgac ggagaggacg ggagcgggaa acggattttt tttgccgtat ggttcagtgg
     1321 tcatgatgat ggaggggggg tgaaaaagaa tattaccagc ggataacggc ggcggaccag
     1381 gtaagcccgg cgccgaatgt gaccaggaca acgttgtctc ccttgccgaa tctgccggaa
     1441 cggcgcgctt cgtcaaaggc gatggcgacg gcggccgcgg acgtgttgcc gtacttctca
     1501 atgttcacga acaccttatc ttcaggaatg gaaaggcgct gcgctacggc gttaatgatg
     1561 cggagattgg cctggtgggg aataatcagc tggatatcgt ccgccgtgag gccggcgcgt
     1621 tcaatgacgc tggcggcgga agccttcatg cgcgtgacgg catggcggaa ggtctcattg
     1681 ccgcgcatgg ccagggtagc caggcgttca tgggcgtttt ccgctgtggt ggggcagcgg
     1741 gagccgccgc ccggaatctg gagcaggtca tgcagggagc cgtccgaacc gatatccgtg
     1801 gcaaggacgg aaccttctcc ctcttcccct gtggaagcct ggagcacggc tgccccggcg
     1861 ccgtccccga acaggacgca ggtgctgcgg tcctcccagt tgacgacggt ggaaagtttt
     1921 tcagcggcga tgatgagcgc cgttttcatc tggccggtgc cgatgtactg gacggcggtc
     1981 ttcatggcga acaggaagcc ggagcaggcg gcggaaatgt caaaggcggc ggcattcttg
     2041 gcgccaaggg catcctggat atagcaggaa gtggaagggg tgaacgtatc cggcgtgatg
     2101 gtggcgacga taatcaggtc aatatcctcc ggcttcagcc cgcaggattc cagagcgcgc
     2161 tgggcggcct tcgtccccat atgggaagtg tactcgtcag gggcggcgat gcggcgttcc
     2221 acaatgcccg tgcgttcaat aatccattcg tgggaagtgt ccaccatctt ctccagatcc
     2281 gcattggtca ggcggcgttc cggaacatag gaacccgtac cgataatctt gacgggcagt
     2341 cttttaaagg gagtatcaga atgtgcggcc atagctttgg gcgtatgatg aacagaaaac
     2401 gtcctgacgt tttatgtgga aacagtctaa aagaaagcgg gcctgatgac cagcacaaaa
     2461 cgcagcactc cttccatgcc gtaaatgaat ggcctacaac agccacccct ggctgttggc
     2521 gggcggcgcg gctcccatct tgagataggc ggaaggcatc gccacgcgcc ccctgggggt
     2581 gcggctgata tacccctgca taatcaggaa aggctcgtgc acgtcctcca gcgtggaagc
     2641 gtcctcccct acggccaccg ccagggagga aaggcccacc gggccgccgt tgaacttgta
     2701 aatcatcgct tccagcaggc gcttgtccat ctcgtccagg ccatcgtcgt caatctcaat
     2761 catggtcagg gcgtcgtgcg ccaactgctc cgtaatcacg ccgtcgccgc gcacctgggc
     2821 gtaatcccgc acccagcgca gcagggaatt ggccacgcgg ggcgtgccgc gggagcgcag
     2881 cgcgatctgc agggcgccct ccgggtccac gggcacgttc agcagccccg cggaccgttc
     2941 gataatggcg cacagctcct cccgcgtata ataatccagt cggttcacta ggccgaagcg
     3001 ggaacgcagc gggctggtca gcatcccggc gcgggtggtg gcccccacca gggtgaactt
     3061 gggcaggttg agctggatgg agcgggcgtt cggcccctgg tcgatgataa tatccagccg
     3121 gaaatcctcc atggccgggt acagatactc ctcaatggcc gggtgcaggc ggtggatctc
     3181 gtcaataaac agaatatccc ccttttccag attggtcagc acgccggcca ggtctcccgc
     3241 cttttcaatc tgggggccgg aagtcgtatg aatgcggcag ccgacggcat tcgcgataat
     3301 attggcaagc gtcgtcttcc ccaggcccgg gggaccgctc aacaggatgt gatccagcac
     3361 gtcgtcgcgc tgtctggcgg cttccaccat cagcatcagg cggtccttaa tcttctcctg
     3421 cccgcagaac tcgctgaaag cgggtggtct gagggacaaa tcaaaagcgg aggagggggc
     3481 ctccgtggtg cgtgtgtaaa aggactctcc catgcgcagg actagcaaac ctgtctttgg
     3541 ggcatccgtc aagggggaag tgcggtcggg aaacgccttt tccgggacat gccggaagcc
     3601 tttggccttt tttgaggacg ggaggaatgc ggagccgcgg aaatgatgat actcggtgaa
     3661 cggcatgtcc cgccttctct ccttacagcg tgggttctca tttctgccgc gttttcccgg
     3721 aactcccgga accctgggtt tctggaatcc ttcggtcttc tcttcctgtc tcgctcctga
     3781 aat
//
LOCUS       DAOMSU010000205         3761 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2385_length_3761_cov_3.462763, whole genome shotgun sequence.
ACCESSION   DAOMSU010000205 DAOMSU010000000
VERSION     DAOMSU010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2385_length_3761_cov_3.462763"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="PK077_08325"
     CDS             complement(<1..849)
                     /locus_tag="PK077_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRN24342.1"
                     /translation="MAKREDIRKILIIGSGPIIIGQACEFDYSGTQACKALRAEGYDV
                     VLVNSNPATIMTDPGMADHTYIEPLTVDRITAVIEKERPDALLPNLGGQTALNLASKL
                     HEEGILKQYGVEVIGVDLEAIARGEDRIIFKETMDRIGVPVAKSEPVYSVEEAEKVAS
                     ELGYPVVLRPAYTMGGTGGGIVYNVEELRQVVPRGLAASLINQVLVEECVLGWEELEL
                     EVVRDAKGQKITVCFIENVDPMGVHTGDSFCVAPMLTVPQKVQDKLQDYSYRILDAIG
                     VTGGTNV"
     gene            1122..1910
                     /locus_tag="PK077_08330"
     CDS             1122..1910
                     /locus_tag="PK077_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24343.1"
                     /translation="MICCLGLLCSAASAADLSSTGRGSAVPSGEASWYQALRTGLSQF
                     RKGGGYKTNREAMEALAQKTCRWDPRTRRPVFLLRNATPSFCSSACYLLLLKSLQIWD
                     SAQPRPVISERAWLALIPRLGQHDGEGPWGWANANGPGLAVLVHRLGAGINFEDWRKA
                     RPGDFMKIFWTDRIGRRESGHLTVLVKDGGDQVTFWSSNIPDGYGARTVPKSRIRRVI
                     FTRITRPERFNLAPSVGSHPWLSSLLRQEVGMKEVRRHSGMQNP"
     gene            1923..2441
                     /locus_tag="PK077_08335"
     CDS             1923..2441
                     /locus_tag="PK077_08335"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR04274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HRN24344.1"
                     /translation="MKKCSFPPSVTPDCSVLVLGSLPGDESLRQAQYYAHPRNAFWKI
                     MGSLLGFDPSLPYGERLSLLNRGGVGLWDVVASGVRPGSLDQHISQEQPNDIAALLDS
                     FPGIGVVCCNGTASHKYLKRYFPELFLRETLSVIQMPSTSPAAARLTYEQKFRAYEEI
                     MAPLLRKEKQEY"
     gene            complement(2501..3469)
                     /locus_tag="PK077_08340"
     CDS             complement(2501..3469)
                     /locus_tag="PK077_08340"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HRN24345.1"
                     /translation="MAFTASQAFDLLRTAFSRNRLPHALLIVGDEHQGASRLILQLLE
                     LINGIRADHLDSVRDEYCRLVRPKSKSRRILRDDIRAVEPFLQQRAAEGKWKIAVFMD
                     AERMNDEAANAFLKTLEEPPGQCLLILSTSQPDQLLQTIISRCVRVNLLQSGEFRLTP
                     IQEALLPHWLETCRNLNNDLAALAFRSRFMDVMAEAKAAITKNLNQALKDEAREAAQG
                     TDASDWEARNKDANAAQIETEYLEQRNQALELLISWFGQAALIASGAPEVAPIHPEVR
                     TLAAQMPVNELLKRMEALNRLRDDLNFNIHEALALDVHLLAAVGSC"
     gene            3556..>3761
                     /locus_tag="PK077_08345"
     CDS             3556..>3761
                     /locus_tag="PK077_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HRN24346.1"
                     /translation="MEQNDISSRIVFADRGKVNVEKGVEFAPKFDQNGLIPALAMDHI
                     THEPLMLAYMNAESLRMTMELGEAV"
BASE COUNT          712 a         1129 c         1127 g          793 t
ORIGIN      
        1 cacgtttgtg cctcccgtga cgccgatggc gtccagaatg cggtaggaat aatcctgaag
       61 cttatcctgc actttctgcg gaacggtcag catgggagcc acgcagaagc tgtccccggt
      121 gtgcacgccc atgggatcca cgttctcaat aaagcagacg gtaatcttct gtcccttggc
      181 gtcacggacg acttccagtt ccagttcctc ccatcccagc acgcattctt ccaccagcac
      241 ctggttgata agggaggcgg ccaggccgcg ggggactacc tggcgcagtt cctccacgtt
      301 ataaacgatg ccgccgcccg tgccgcccat ggtgtaggcg ggacgcagca cgacggggta
      361 gcccagttca gaggccacct tttcggcttc ctccacggag tagacgggtt cggacttggc
      421 gaccggcacg ccgatcctgt ccatggtttc cttgaaaatg atgcggtctt cgccgcgggc
      481 gatggcttcc aggtccacgc cgatgacttc cacgccgtac tgtttcagga tgccttcctc
      541 atgcagcttg gaagccaggt tcagggccgt ctggccgccc aggttgggca atagggcatc
      601 cgggcgttcc ttctcaatca cggccgtgat gcggtccacg gtcagaggct caatgtaggt
      661 gtggtccgcc atgccggggt ccgtcatgat ggtggcgggg ttggaattca ccagaacgac
      721 gtcgtaacct tcggcgcgga gggccttgca ggcctgggtg ccggagtagt cgaactcgca
      781 ggcctggccg atgatgatgg ggcctgagcc gataatgagg attttgcgga tatcttcgcg
      841 ttttgccatg gtatgatgcc tctatgtggt ccgtggtggt taaaaataaa tgccggaaac
      901 aaggtccgcc caaggtgcgt tgcctctccg ctccgcatac ccggcgcgtg acctgacgca
      961 cagtacggga cgccgggcga taagtccaga caaaacaaca ggaattccgg ggaaaaggct
     1021 atggccttct ttccttttcg tacagtgccg cacgcaatcc gcccttgata ttttccaccg
     1081 tccgtatacc ctgcggagca aggatgagga acgccctgac attgatttgc tgtcttggat
     1141 tgctgtgctc cgcagcatcg gctgcggacc tttcttccac agggcggggg agcgccgtgc
     1201 cttcggggga ggcttcctgg taccaggccc tgcggaccgg actttcccaa ttccggaagg
     1261 gtggaggcta caaaaccaac agggaagcca tggaagccct ggcgcagaaa acctgccgct
     1321 gggacccccg cacgcgccgt cccgtttttc ttctccgtaa cgccacgccc tccttctgtt
     1381 cctccgcctg ttacctgctc cttttgaaat ccctgcagat atgggactct gcacagcctc
     1441 gccccgttat ttccgaacgc gcatggctgg ccctcattcc ccgcctcggc cagcatgacg
     1501 gcgaagggcc gtggggatgg gcgaacgcca atggccccgg cctggccgtc ctggtgcaca
     1561 ggctgggagc cggcatcaac tttgaggact ggaggaaggc ccgccccggc gacttcatga
     1621 aaattttctg gacggaccgc atcgggcgcc gggaatccgg ccatctgacc gtcctggtga
     1681 aggatggagg ggaccaggta acgttctggt cttccaacat tccggacggt tacggagcca
     1741 ggacggtgcc caaatcccgg atcaggcgcg tcattttcac gcgcatcacc aggccggaac
     1801 ggttcaacct ggccccctcc gtgggaagtc acccgtggct gtcctccctg ctccggcagg
     1861 aagtgggcat gaaggaagtg cgcaggcatt ccggcatgca gaacccctga ccgtttcccg
     1921 tagtgaaaaa atgctccttc cctccttccg tcaccccgga ctgttccgtg ctggtgctcg
     1981 gttccctgcc gggagacgaa tccctgcgtc aggcccagta ttacgcccat ccccgcaacg
     2041 cgttctggaa aatcatgggg tcccttctgg ggttcgatcc ctccctgcca tatggggaac
     2101 gcctgtctct gctcaaccgg ggcggggtgg ggctgtggga tgtggtggcc tccggcgtca
     2161 ggcccggcag cctggaccag cacatctccc aggaacagcc caatgacatt gcggccctgc
     2221 tggatagctt cccgggcatc ggcgtggtct gctgcaacgg caccgcttcc cataaatacc
     2281 ttaaacggta ttttcccgaa ctgttccttc gggaaacgct ttccgtcatt caaatgcctt
     2341 ccaccagtcc ggcggcggcg cggctgacgt atgagcagaa attccgcgcc tatgaggaaa
     2401 tcatggcgcc cctgctccgg aaggaaaagc aagaatactg accttctgcg ccttttccaa
     2461 tgccgtgcgc ggcccgacgg taacaggcgg gggtgttcgg tcagcaggac ccgacggccg
     2521 ccagcaggtg gacatccagg gccagggctt cgtgaatatt gaaattcaaa tcatcacgga
     2581 gcctgttgag ggcctccatg cgcttcagca attcgttcac gggcatctgg gctgccagcg
     2641 ttcgcacctc cggatgaatc ggagcgactt ccggggctcc ggaggcaatc agggctgcct
     2701 ggccgaacca gctgatgagc aattccaggg cctgattgcg ctgttccaga tattccgtct
     2761 ctatctgggc ggcgttcgcg tccttattgc gggcctccca gtcggaggcg tccgtaccct
     2821 gggccgcttc cctggcttca tctttcagag cctggttcag gtttttggtg atggcggcct
     2881 tggcttctgc catgacgtcc atgaacctgc tgcggaaggc cagagcggcc aggtcattat
     2941 tcagattgcg gcaggtctcc agccagtgcg ggagcagggc ctcctgaata ggagtgagcc
     3001 tgaattctcc ggattgcagc agattgacgc gcacgcagcg ggaaataatt gtctggagaa
     3061 gctgatccgg ctgggaggtg gacaaaatga gcagacactg gccgggcggt tcctccaggg
     3121 ttttcaggaa agcgttggcg gcctcgtcat tcatccgctc cgcatccatg aacacggcta
     3181 tcttccattt cccctccgcc gcgcgctgtt gcaaaaaggg ttccacggcg cggatatcat
     3241 cccgcaggat gcggcgcgac ttggacttgg ggcgcaccag ccggcaatac tcgtccctta
     3301 cactgtccag atggtctgcc cggatgccgt ttatcagctc cagcagttgc aggatcaggc
     3361 gggaggctcc ctgatgctcg tctcccacga tgagcagggc atggggaagc cggttgcggg
     3421 agaaggcggt gcgcagcaga tcgaacgctt gggatgccgt aaatgccata tgctcctatg
     3481 aaaacatttc gttgcgtcag ggcaacagaa aagattggta tatgtcggat atttcatgta
     3541 ctaagggggc cggttatgga acaaaatgac atctcatccc ggattgtctt tgccgaccgc
     3601 ggcaaggtga atgtggaaaa aggcgtggaa tttgccccca aatttgacca gaacggcctg
     3661 attccggccc tcgccatgga ccatattacc catgaaccgc tgatgctggc ctatatgaat
     3721 gcggaatccc tgcgcatgac catggaactg ggggaagccg t
//
LOCUS       DAOMSU010000206         3731 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2422_length_3731_cov_2.200762, whole genome shotgun sequence.
ACCESSION   DAOMSU010000206 DAOMSU010000000
VERSION     DAOMSU010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2422_length_3731_cov_2.200762"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PK077_08350"
     CDS             <1..610
                     /locus_tag="PK077_08350"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRN24347.1"
                     /translation="MLLCNPHNPAGRVWTREELEEMAAICRRNGVFVVADEIHCELTY
                     PGHPYTPFASLSEDALHHSVTCISPSKAFNLAGIQIANIVAADEEVRKRIDKAININE
                     VCDVNSFAVDALEAAYNGGEDWLEELKLYLYGNYEIVRDMLAERLPQLRVVPLEGTYL
                     VWIDCSALGLPSAEIVRLLEDVRGNGGVLHSPERRLSQEAAA"
     gene            744..1739
                     /locus_tag="PK077_08355"
     CDS             744..1739
                     /locus_tag="PK077_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRN24348.1"
                     /translation="MTRRTWLQSSTAALACLALPEYALGAVAEKGGASRVWMTKEISP
                     EALVRIYEALGRPAGGKVAVKISTGEPGGRNFLSPALIKDLVRRVNGTIVECNTAYGG
                     KRSRTEDHLQAAKDHGFSDIARVDIMDAEGEFTIPVRDRKHLEYDIVGDHLKNYDFMV
                     NLAHFKGHAMGGFGGVIKNQSIGVASAAGKAYIHSAGKTRDVSSVWNNLASQDDFLES
                     MAASAQAVADYFGDRILYINVMNNLSIDCDCDSHPHAPEMKDIGILASLDPVALDQAC
                     LDLVYAVRPSEGNDNRPLVERIESRHGRHTVEYAEKIGLGSRKYELKELKPQQAV"
     assembly_gap    1757..1766
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1812..2660
                     /locus_tag="PK077_08360"
     CDS             1812..2660
                     /locus_tag="PK077_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRN24349.1"
                     /translation="MEKVMLNNGVTMPVLGFGVFQIPDAKECERCVLDAIEVGYRSID
                     TAQIYGNEEAVGRAAEKSGVPREELFLTTKVWISNGGYEKAKASIDESLRKLRTDYLD
                     LLLIHQPFNDYYGTYRAMEEANRAGKVRAIGVSNFYPDRFVDLAEFCEIRPAVNQVET
                     HVFHQQEKVREVMEKYGTRMESWGPFAEGRNGFFSNPVLKGIGEKYGKTPAQTALRFL
                     IQRGIIAIPKTTHRERMEENFNVFDFALSLEDMGAIARLDRGESLFLCHRDPESILYL
                     INYGKQ"
     gene            2682..>3731
                     /locus_tag="PK077_08365"
     CDS             2682..>3731
                     /locus_tag="PK077_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRN24350.1"
                     /translation="MIRYSFKIFCVAFSACFCCAGSLLAEDNNPHTDKMVNEKLNLTQ
                     EWDKVFPKSDKVTHRKVSFRNRYGIMLAADLYMPRNVNEKLPAIAVSGPFGAVKEQSA
                     GLYAQTMAERGFLTIAFDPSYTGESGGFPRYVASPDINTEDFCAAVDYLSTRDDMDSE
                     RIGIIGICGWGGMAVNAAAIDTRIKATVTSTMYDMSRVNANGYFDAMDADARYELRKQ
                     LNAQRTADAKSGSYALAGGVPDPLPADAPGFVKDYYDYYKTPRGYHRRSLNSNGGWNV
                     TSALSFINMPLLAYSGEIRSAVLMIHGEKAHSRYFSEDAFKKLKGDNKELMIIPGASH
                     VDLYDNQAGVIPFDRI"
BASE COUNT          847 a          909 c         1117 g          848 t
ORIGIN      
        1 gatgctcctg tgcaatcccc ataatccagc cgggcgcgtc tggacgaggg aagaattgga
       61 ggagatggcc gccatttgcc ggagaaacgg cgttttcgta gtggctgacg agattcattg
      121 cgaactgacg tatccgggcc atccctatac gccgttcgcc tccctctccg aggacgccct
      181 gcaccattcc gtcacctgta tttcccccag caaggcgttt aatctggcag gcattcagat
      241 agccaatatc gtcgcggcgg atgaagaagt tcggaagagg attgacaagg ccatcaacat
      301 caatgaggtg tgtgacgtca attcttttgc cgtagatgcc ctggaagcgg cttataacgg
      361 aggggaggac tggttggagg aactgaagct ctacctttac gggaattacg agattgtccg
      421 ggatatgctg gcagaacgtc tgccgcagct tcgtgtggtg ccgttggagg gaacgtacct
      481 ggtctggata gattgttccg ctctggggct tccctccgca gagattgtcc gcctgctgga
      541 agatgtacgg ggaaacggcg gggtgcttca ttcgcctgaa cgtcgcctgt cccaggaagc
      601 tgctgcttga ggggctggaa agaatcatcc gcacgctgtg cggcctcatt tccggaacac
      661 ggacgtgccc gtcgggtcct gcaggctctt aacagggaga acggttatta atcatcaaca
      721 atcaagttgc catggaaaat aagatgacga gaagaacatg gctccagtct tccaccgcag
      781 ccctggcatg cctggctttg ccggagtatg ccctgggcgc ggttgcggag aagggtggag
      841 cttccagggt ctggatgacg aaagaaattt ctccggaggc tctcgtgagg atttacgagg
      901 ctctggggcg tccggccggg ggaaaggtag ctgtcaagat cagcaccggg gagccgggag
      961 gccgcaattt tctgagtccg gccttaatca aagacctggt gcgccgggtg aacggcacca
     1021 tcgtggaatg caatacggct tacggaggca aacgctcccg gacggaggac catttgcagg
     1081 ctgcgaagga tcacggtttt tccgatattg cgcgggtgga tatcatggat gcggaagggg
     1141 agttcactat cccggtgagg gacaggaagc acctggaata cgatatcgtg ggggatcatt
     1201 taaagaatta tgatttcatg gtcaatctgg cccatttcaa agggcatgcc atgggcggct
     1261 tcggcggtgt gatcaagaac cagtccatcg gtgttgcctc ggcagccggg aaggcgtaca
     1321 tccattccgc cggaaagacg cgggatgttt cctccgtgtg gaacaatctg gccagtcagg
     1381 atgatttcct ggagtccatg gcggcttccg cgcaggcggt ggcggattac ttcggggaca
     1441 gaattttgta catcaatgtg atgaataatc tgtccattga ctgtgattgc gattcccacc
     1501 cccatgcgcc ggaaatgaag gacatcggta ttctggcctc ccttgatccg gttgctcttg
     1561 accaggcctg cctggatctc gtttacgccg tcaggccgtc cgaagggaat gacaacaggc
     1621 ccctggtgga gcgtattgaa agccgccatg ggcggcatac ggtagagtat gccgagaaga
     1681 taggtcttgg cagcaggaag tatgaactga aagagctgaa accgcagcag gccgtttagt
     1741 cggtcatttc ctttttnnnn nnnnnnctaa tttttctatg atgtgtaaac cggaggtgag
     1801 aaaaaactgt gatggaaaaa gtgatgttga acaatggcgt tacgatgcct gtcctgggat
     1861 ttggcgtttt tcagattccg gacgctaagg agtgcgaacg ctgcgtgctg gatgcgattg
     1921 aggtcggcta ccgttctatt gataccgcgc agatttacgg caatgaggaa gccgtaggcc
     1981 gcgcagcgga gaagagcggg gttccgcggg aagagttgtt tcttaccacg aaggtgtgga
     2041 tttccaacgg cgggtatgag aaggcgaagg cgtccattga cgaatccctg cgcaagctgc
     2101 gcacggatta tctggatctg ctgctcatcc accagccttt caacgattac tacggcactt
     2161 accgggccat ggaagaagcg aaccgggccg gaaaggtacg cgccattggc gtgagcaatt
     2221 tttatccgga ccgttttgtg gacctggcgg agttctgcga gatcagaccc gccgtcaatc
     2281 aggtggaaac gcatgtgttc caccagcagg aaaaagtccg ggaggtgatg gaaaagtacg
     2341 gtaccagaat ggaatcctgg gggccttttg cggaaggtcg caatgggttt ttctccaacc
     2401 ccgtgttgaa gggcattgga gagaaatacg gcaagacccc ggcgcaaaca gccctgaggt
     2461 tcctgattca gcgtggaatt atcgccattc cgaagacgac ccaccgggaa aggatggagg
     2521 agaatttcaa cgtctttgat tttgcccttt ccctggagga tatgggcgct atcgccaggc
     2581 tggaccgcgg agagagcctt ttcctgtgcc accgggatcc ggaatcgatc ctgtacctga
     2641 tcaattacgg gaagcagtaa ttcgcatgaa gttccagaaa gatgatacgt tactcattta
     2701 aaatattttg tgtggctttt tccgcctgct tttgctgtgc ggggagcctg ttggcagaag
     2761 ataataaccc ccatacggac aagatggtga acgagaagct gaatttaacg caggaatggg
     2821 acaaagtgtt tcctaaaagc gacaaggtaa cccaccgcaa ggtaagtttc cgcaaccgtt
     2881 atggcattat gctggctgcg gatttgtata tgccccggaa tgttaacgag aaattgccgg
     2941 ctattgccgt ttccggccct ttcggagctg taaaggaaca atctgcgggc ctttatgccc
     3001 agacgatggc cgaacgaggt tttttgacga tcgcgtttga tccctcgtat acgggagaaa
     3061 gcggaggatt tccccgctat gtcgcgtctc cggatatcaa tacggaggat ttctgcgccg
     3121 ccgtcgatta cctttccacc cgcgatgaca tggattcgga acgtattgga atcattggca
     3181 tttgcggctg gggcggcatg gcggtcaatg cggcggctat cgacacccgc atcaaggcaa
     3241 cggtaacctc cacgatgtac gatatgagcc gcgtgaacgc gaacggctat ttcgacgcga
     3301 tggatgccga cgcccgttat gagcttcgca aacaactgaa tgcccagcgg acggctgatg
     3361 cgaagagcgg ttcttatgcc ctcgcggggg gcgtgcctga tcctctgcct gcggatgccc
     3421 ccggatttgt gaaggattat tacgattatt ataagacgcc ccgcggctat cacaggcgtt
     3481 cgctcaattc aaatggcgga tggaatgtca cttcggcgct ttccttcatc aatatgcccc
     3541 tgctggcgta cagcggtgaa atccgcagcg ccgtgctcat gattcacgga gaaaaagccc
     3601 attcgcgcta tttcagcgag gacgccttca agaagttgaa gggggataac aaggagctga
     3661 tgatcattcc cggcgcgagc catgtggatc tttatgacaa tcaagccggt gtcattcctt
     3721 tcgacagaat c
//
LOCUS       DAOMSU010000207         3720 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2439_length_3720_cov_2.501228, whole genome shotgun sequence.
ACCESSION   DAOMSU010000207 DAOMSU010000000
VERSION     DAOMSU010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2439_length_3720_cov_2.501228"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(153..1058)
                     /gene="miaA"
                     /locus_tag="PK077_08370"
     CDS             complement(153..1058)
                     /gene="miaA"
                     /locus_tag="PK077_08370"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRN24351.1"
                     /translation="MTLFLAGPTGSGKSAVAVELAEMLDAEIVSSDAYQVYRELPILT
                     AAPSPADRERVPHHMVSIIPVQRNWNATEHYHRAMRCMEEIRARGKIAIVTGGSGLYF
                     KFLSHGLSEAPPGDAALRAAFANCSTEALYARLSSLDPEGAASTDAANRRYVERNLEI
                     VLAGGKPLSFWKRNWLAPPRGPGWVISRDVPELDGRIALRTARMMQEGAVEEAASLGP
                     CSATAERTLGLALIRSMLRGKISRENCQIQLALATRQYAKRQRTWLKREQWLRKLPAS
                     PADSPRDLAERIMKELESSPSFIRR"
     gene            complement(1142..1882)
                     /locus_tag="PK077_08375"
     CDS             complement(1142..1882)
                     /locus_tag="PK077_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24352.1"
                     /translation="MFPKKIKACILFLLLTVPAALGAVWCAKHAYRYHLAVHPLPYSP
                     EQGALLEKHRQDKRNARLNPHAPVLFLGSSTMEFWLKEGKHSWETWFSPLGCLNLGTR
                     SETTGNLLWRLNDGLTSPLAPKIIVLYSGVNNPGVQKSLGWTAFQGNVAIVKKIREIY
                     SPETTAIIVIPPLIAQKGSRMETFRNCLLQHDFGENVHVLPLHFFLPGKDASLYTWDG
                     LHLNALGYEAISPPLFRFLQQLLSRSSF"
     gene            complement(1863..2975)
                     /locus_tag="PK077_08380"
     CDS             complement(1863..2975)
                     /locus_tag="PK077_08380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRN24353.1"
                     /translation="MTPQFTKFPDAPKASSREHWIDISRFLAAYLIVCVHAPAFSFSS
                     MFQLPVFNGRVAFFLILAGYFMGRNNSWGKAVKRFVTLLIPFLFWNVIISFFYPGKEE
                     IMNMGVLEYLCNLVGFRSFFTPDLTLPIDGLIGRPFSPATWFLRDIILCSLISPLLLK
                     FKYPVYALILICFCTDLLDISDSLITLSPQPVATFCLGLLLSSIKLASVKTFYRNSFT
                     PYVLLSVGIGFLLSITNYLSVCQNNPEWNMHKILASYGVWNISWPAVRLNSLCPVFGA
                     LLLMTLGIWLEKKIPVARKLSNYAPACFLIFVLHYPLIMLIPSHIWKQSFFAPFVLIP
                     AIIFLIVAFYLFLRKFFPACLPYVANDRITHVPKKN"
     gene            3386..3658
                     /locus_tag="PK077_08385"
     CDS             3386..3658
                     /locus_tag="PK077_08385"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HRN24354.1"
                     /translation="MNTISSTAARQSISRTLAAVLNDREPVRIVNKRLGAVIIMPEDE
                     FNSWQETIYLLKCPANARRLLDGMDVLNRRQGTVTKSVEELESHSC"
BASE COUNT          884 a          875 c         1130 g          831 t
ORIGIN      
        1 cggttattcg gcggagcttt cgacatatct ctggctttta agcgggtggc gtaagctcaa
       61 tttgccatgc gtatcctgtc cccatggttc gtggcggcgt gcgttatgaa tggcgtctgg
      121 catttttccc gctgtgggac ggttccggat gtctaacggc gtatgaaaga gggtgaagat
      181 tccagttcct tcatgatgcg ttctgcaaga tcccggggag agtcggcagg ggaagccgga
      241 agtttccgga gccattgttc gcgtttcagc caggtgcgct gtcgtttggc gtattgccgg
      301 gtggcgaggg ccagctgaat ctggcagttt tcacgggaga ttttcccgcg cagcatgctt
      361 cgtatcaatg ccaggcccag ggtgcgttcc gctgtggcgg agcatgggcc gagggaagcc
      421 gcttcctcca ccgcaccctc ctgcatcatg cgtgcggtac ggagggcgat tcttccgtcc
      481 agttcaggta catccctgct gatgacccag ccgggcccgc gtggcggggc cagccagttc
      541 ctcttccaga aggaaagcgg tttccctccg gcaaggacga tttccagatt gcgttccaca
      601 taacgccggt tggcggcatc cgtcgaggcc gctccttccg ggtccaggga agagagcctg
      661 gcgtatagcg cttccgtgga acagttcgcg aaagcggcgc ggagagcagc gtcccccggc
      721 ggcgcttccg acaggccgtg ggagaggaat ttgaagtaca ggccggaacc tcccgtgacg
      781 atggctattt tcccgcgtgc gcggatttct tccatgcagc gcatggcgcg gtggtagtgt
      841 tccgtggcat tccagttcct ttggaccgga atgatggaaa ccatgtggtg gggaacccgt
      901 tcccggtctg ccggggaagg cgccgccgtc aggatgggca gctcccggta cacctggtaa
      961 gcgtcggaac tgacgatttc tgcgtccagc atctccgcca gttccacggc taccgcggat
     1021 ttgcccgatc cggtgggacc tgccagaaac agggtcatgg gaagtgttgc ctgatggggt
     1081 ggagaagatt caatcatcac gtggagtaat ggcggataaa gtttgttgtt tctgtgaagc
     1141 cctaaaagct gctcctgctt aaaagctgtt ggaggaagcg gaaaagcgga ggcgaaatgg
     1201 cttcatatcc cagggcgttg agatggaggc cgtcccaagt ataaaggctg gcgtcttttc
     1261 ccggaaggaa gaaatggagg gggaggacat ggacattttc tccaaagtcg tgttgcagca
     1321 gacaattcct gaaggtttcc atgcgcgacc ctttttgtgc gatgagcggg ggaatgacaa
     1381 tgatggcggt cgtttccggg ctgtaaatct ccctgatttt tttgacgatg gccacgtttc
     1441 cctgaaaagc cgtccatccc aggctttttt gcactcccgg attgttgact cccgaataca
     1501 ggacgattat tttgggcgcc agcgggcttg tcaggccgtc attgaggcgc cagagcaggt
     1561 tgcccgtagt ctcgctcctg gttcccaggt tgaggcatcc cagaggggaa aaccaggttt
     1621 cccaggaatg tttcccttct ttcagccaga attccatggt ggaggaaccc aggaacaaca
     1681 cgggggcatg gggattcagc cgggcgttgc gtttgtcctg ccggtgtttt tccagcaggg
     1741 cgccctgttc cggggaatag ggaaggggat ggacagccaa atggtagcgg taggcgtgtt
     1801 tggcgcacca gacggcgcct aatgcggccg gaacggttaa aagaagaaag aggatacagg
     1861 ccttaatttt ttttgggaac atgggtgatg cggtcgttgg cgacgtaggg caggcaggcc
     1921 gggaagaatt ttctcaggaa aagatagaat gcgacgataa ggaaaatgat ggccggtatt
     1981 aatacaaagg gagcgaaaaa ggattgtttc cagatgtggg aggggatcag catgatcaaa
     2041 ggataatgaa gaacgaatat caggaaacag gcgggagcat agttggacag ctttctggca
     2101 acgggaattt tcttttccag ccagattcca agggtcatga gaagaagcgc cccgaagact
     2161 gggcagaggg aattcaaacg gacagccggc caggagatat tccatactcc ataggatgcc
     2221 agtattttgt gcatgttcca ttccgggtta ttttggcaga cgcttaaata gttggtgatg
     2281 ctgagcagaa aaccgatgcc tacggagagc agaacgtagg gagtgaagga atttctataa
     2341 aatgttttta ctgaggccag cttgatggaa gacaggagaa ggcccaggca gaacgtggcg
     2401 acgggttggg gagaaagggt aatgagcgag tcggaaatat ccagtaagtc cgtacagaaa
     2461 cagatcagga tgagggcata cacggggtac ttgaatttca gcagcagggg agaaatgagg
     2521 gagcagagaa taatgtctct caaaaaccag gtggccggag agaaaggtct gcctatcagc
     2581 ccgtcaatgg ggagggtgag gtcaggcgtg aagaaagacc ggaaccctac aagattgcat
     2641 aaatattcca gtacccccat attcatgatt tcctctttcc ccggatagaa gaaagaaatg
     2701 atcacgttcc agaaaagaaa gggaatgagc agcgtcacga accgcttgac cgcttttccc
     2761 caagaattat tccggcccat gaaatagcct gccagaatca ggaaaaatgc tacgcggccg
     2821 ttgaaaaccg gcagttggaa catgctggag aaagaaaagg ccggagcgtg gacgcacaca
     2881 atcaggtatg ccgcgaggaa cctggatatg tcaatccagt gttcccggga tgatgctttt
     2941 ggcgcatcgg ggaattttgt gaattgcggc gtcatggcgt agccgcacca taaataacgg
     3001 tgagggtctc cgcaataata ttattactcg ccggaaaaac agttgaggac cggcaaggga
     3061 ataatccccg ctttgcagcg ttccgcgccg ggaagggaaa tgctgaaagg taaggggcgt
     3121 ttgcgctctg ctgttgatga tgataacggg gtattgcccg catcaatctt tacctgagct
     3181 ctgggttgtt gccggaacag tctttcaacc tcctggaggg agggcatttg ctcctgaatg
     3241 aatttcatat caaagtcctt atcctgtacc cggagcgctt tcttgggcaa tggggcgtaa
     3301 acatgattaa tgacgctttg caggatgcct gagaaaatac agaagaatgg cttacttatg
     3361 tacatggttc atgtacactt tattcgtgaa taccatttca agcaccgccg cgcggcaatc
     3421 catatctagg acgcttgccg ccgtcctgaa tgaccgtgaa cccgtccgca tcgtcaataa
     3481 acgcctgggg gcggtcatca tcatgccgga agatgaattc aattcctggc aggaaacaat
     3541 atatttgctc aaatgtccgg ccaatgcccg ccgcctgctt gacggcatgg acgttctcaa
     3601 ccgcaggcaa ggcacggtaa cgaagagcgt tgaagaattg gagagtcact cctgctaacc
     3661 tccggctttc ccatgaaaag aaatattgtc ttcgcgcccc cggcatggga agattttcaa
//
LOCUS       DAOMSU010000208         3715 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2444_length_3715_cov_2.731148, whole genome shotgun sequence.
ACCESSION   DAOMSU010000208 DAOMSU010000000
VERSION     DAOMSU010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2444_length_3715_cov_2.731148"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(320..970)
                     /locus_tag="PK077_08390"
     CDS             complement(320..970)
                     /locus_tag="PK077_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRN24355.1"
                     /translation="MRRVFISGGHGGLARAIVECFSAAGWETDAPSHAALDVGDRSAV
                     RRWFDCHAAYDLAVCAAGITRDRPFLKQTEEEWDEVMNVNVTGAAWCGRCAAAAMVRE
                     KREGQVVMIGSYAALRPAPSQAAYAASKAALAGLVKSLAREWGKDGIRVNLVLPGFML
                     TEMTAVLRDSVKEAALSRHVLNRLNTPEQAAAFILFLQDVLTAASGQVFDLDSRIV"
     gene            complement(998..>2381)
                     /locus_tag="PK077_08395"
     CDS             complement(998..>2381)
                     /locus_tag="PK077_08395"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRN24356.1"
                     /translation="KFEVREYSDAEYREELDRLREEFTPLVLFCREHGRAMRIGSNHG
                     SLSDRILNRFGDTPEGMVESAIEFAQIARDLDYHSLVFSMKASNVKVMVAAYRLLVER
                     MNALGPDWNYPIHLGVTEAGGGEDGRIKSAVGIGSLLTDGIGDTLRVSLTEDAVREVP
                     VAYRLSNPFQPSERSDDPVSFPEPELSYDPLKFSKRQGGLAMCYGVRLGWEQPVRVAV
                     PDAGFYALQTEREAMGDMMPELSLGQLDAIEVDPRCDADLEPLKELAEPSIVTVKNGL
                     AMEPVYAFRLLAARIEDRHLILLKDTLVPGSVSGEDVPLTAARNIGSLLCDGIGDAVL
                     IQGESDPRLASFLGFNILQATGTRLTRADYVSCPSCGRTLYNIQEATARIRKATEHLK
                     GVKIAVMGCIVNGPGEMADADFGYVGGAPNKINLYVKHTPVKFNIPQEEAVERLVDLI
                     KEYGRWVDPK"
     assembly_gap    2382..2407
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2408..2770)
                     /locus_tag="PK077_08400"
     CDS             complement(<2408..2770)
                     /locus_tag="PK077_08400"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HRN24357.1"
                     /translation="MQSSYCPSPYRYTRRVTREVMVGNVGVGGSNPIRIQSMLTSDTR
                     DTDACVKEALELAEAGCEIIRLTAQTKAYAANLENIARELRAAGCHVPLVADIHFKPD
                     AAMEAAKWVEKIRINPGNF"
     gene            complement(2947..>3715)
                     /locus_tag="PK077_08405"
     CDS             complement(2947..>3715)
                     /locus_tag="PK077_08405"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRN24358.1"
                     /translation="SAEGPLYEAVTVRTEMSGPFYRADIPEYPGAVYGFPQTDTAELR
                     VNGCAVPVMAEAQKTSGGTSRTYNGVLELKRAAIAGETVVEGRMLRTVETPLLAAAAR
                     TPEQDVPVLGKEQDKYGMEAKGSGGHVEVALNAGEGEALMVPVVYDRGWRVRRNGMEA
                     PVEKVGELMAVRLLKGQNRVVFDYYPPLLKGSLLFSSGAAAVFLLYAWWARRHPESSL
                     RRIVLACGYRLFQCCAAVVLAAVYMGSIVLFIMQSAL"
BASE COUNT          829 a         1264 c          879 g          717 t
ORIGIN      
        1 gatctggaca gcaggattgt ttaataatgg acagcggatt tattatccgc gttctcccct
       61 gctgatgata tatttccaag aaaatcattt ttttaataaa aaagatagcc tgctatgatt
      121 ttgaaaacag gaaatcttga aaaacaaggt ttttccggtc ttttggaaag tgtgtaaaca
      181 tggatagatt cctctttccg tgtttggaat tccatgtttt caacgggaac cggatgtccc
      241 tggtgtatca aattttttct taaattattc ttcttcaata ttaaggcttg aacgcggata
      301 ataaatccgc tgtccattat taaacaatcc tgctgtccag atcgaacacc tggccggaag
      361 ccgccgtaag aacatcctgt aaaaacagga taaacgccgc agcctgttcc ggcgtattca
      421 accggttcaa aacatgccgg gacaaagccg cctctttcac gctatcccgc agcacggccg
      481 tcatctccgt cagcatgaac cccggcaaca ccagattcac gcgaatccca tcctttcccc
      541 attcccgcgc caggcttttc accaggcccg ccagagcagc tttggaagcg gcataagccg
      601 cctgggacgg agccggacgg agcgccgcat aagaaccgat catcaccacc tgaccctccc
      661 tcttctcccg caccatggcg gcggcggcgc atcggccgca ccaggcggct cccgtcacat
      721 tcacattcat tacctcgtcc cactcctcct ccgtctgctt caggaacggc ctgtccctag
      781 taattccggc ggcgcaaacc gccaaatcat acgcggcatg gcaatcaaac cagcggcgta
      841 cggcggaacg gtctcccaca tccagtgcgg catggctggg ggcatccgtc tcccatcccg
      901 cagcggaaaa acattccacg atggcccgcg ccaaaccgcc gtgaccgccg ctgatgaaca
      961 ctctccgcat caaaaaagtt ccctcaacgt taaaacctca cttggggtcc acccaccgcc
     1021 catactcctt gatcagatcc accagccgtt ccacagcctc ctcctgggga atattgaact
     1081 tcacaggcgt gtgcttcaca tacaggttga tcttgttcgg cgcgccgccc acataaccga
     1141 aatccgcatc cgccatctcg ccggggccat tcacaataca tcccatcacg gcaatcttca
     1201 cccctttcag atgttccgtg gctttccgga tgcgggccgt cgcctcctgg atattgtaca
     1261 gggtacgccc gcaggacggg caggaaacgt aatccgcccg cgtcagcctc gttcccgtag
     1321 cctgcaaaat attgaatccc aggaaagaag ccaaacgggg gtccgactcg ccctgaatca
     1381 gcacggcgtc tccaatcccg tcgcacagca gggagccgat attgcgggca gccgtcagcg
     1441 gcacgtcttc cccggaaacg gaaccgggca ccagcgtatc cttcagcagg atcaggtgcc
     1501 tgtcctcaat acgggcagcc agaaggcgga acgcatatac aggctccata gccagcccgt
     1561 tcttcacagt gacaatagac ggttccgcca gctccttcaa cggctccaga tcggcatcgc
     1621 accggggatc cacctcaatg gcatccagct gccccaggga taattcaggc atcatgtccc
     1681 ccatcgcctc ccgttccgtc tgcagggcgt aaaacccggc atcaggaacc gccacgcgca
     1741 caggctgttc ccagcccagg cgcacgccat aacacatcgc cagcccaccc tgccttttgg
     1801 aaaacttcag gggatcatag ctcaactccg gttcagggaa ggaaaccggg tcatcggaac
     1861 gctccgacgg ctggaaagga ttggacaggc ggtaagccac gggcacctcc ctcacagcgt
     1921 cctccgtcag ggaaacgcgc agggtatcac caatgccgtc cgtcagcagg gagccgatgc
     1981 ccacggcgct cttgatgcgg ccgtcctctc cgcccccggc ttccgtcacc cccagatgaa
     2041 tgggataatt ccaatccggc cccagggcgt tcatgcgctc caccagcaat cggtaagcgg
     2101 ccaccatcac cttgacgttg gaagccttca tggaaaacac cagggaatgg taatccaggt
     2161 cgcgggcaat ctgggcaaac tcaatcgcgc tctccaccat cccctccggc gtatcgccaa
     2221 agcggttcag aatgcggtcg gacagggagc catggttgga accgatgcgc atcgcgcggc
     2281 catgctctcg gcaaaacaga accaggggcg taaattcttc cctcaggcgg tccagctcct
     2341 cgcggtattc ggcgtcggaa tactcccgca cttcaaactt cnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnngaa attgcccgga ttaatacgaa tcttctccac ccatttggca gcctccatcg
     2461 cggcatccgg cttgaagtgg atatcggcca ccagaggcac atggcagccg gcagcgcgca
     2521 attcccgggc aatattctcc aaattggcgg catacgcctt ggtctgggcg gtcaggcgga
     2581 taatctcgca ccctgcctcc gccagctcca aagcctcctt cacgcaggca tccgtatccc
     2641 gcgtatcgga cgtcagcatg gactggatcc ggatgggatt ggaaccgccc acccccacat
     2701 tccccaccat gacttcacgg gttacgcggc gcgtatatcg ataaggactc ggacaatagg
     2761 aagactgcat gaaaccactc taccgcgcca gattcccctg tcaaccatcc ttcaaaacgg
     2821 aaagcctttt cccgccaatc cggccaccgg caaaaaggaa acggctacgc cccgcggata
     2881 ttgttccacg ccagcatccc cccgcactca gtccctggcc tatttccctg ccttccgggg
     2941 attccttcac aacgcggact gcatgataaa caacacgatt gaccccatat aaacagccgc
     3001 cagcaccacg gcggcgcagc actgaaacag cctgtatccg caggccagaa caatcctcct
     3061 gagcgaggat tccggatgcc gccgcgccca ccatgcatac agcaggaaga cggcggccgc
     3121 gccggaggaa aacagcaggg aacctttcaa taaaggcgga taataatcaa acaccacccg
     3181 gttttgccct ttcagaaggc gcaccgccat caattccccc accttttcca caggcgcctc
     3241 catcccgttc cgccggactc tccatccccg gtcatacacg accggcacca tgagagcctc
     3301 cccttccccg gcattcagcg ccacctccac atggccaccg cttcctttcg cctccattcc
     3361 gtacttatcc tgctctttcc ccagaaccgg aacatcctgc tccggcgtac gcgccgcagc
     3421 cgccagaagg ggcgtctcca cggtccgcag catcctcccc tccaccacgg tttccccggc
     3481 aatggccgca cgcttcaatt ccaaaacccc gttataggtg cggctcgttc ctccggatgt
     3541 cttctgcgcc tccgccatga cgggcaccgc acatccattg acacggagtt ccgccgtatc
     3601 cgtctgcggg aacccgtaca ccgcgccggg atattccgga atgtccgcgc ggtaaaaggg
     3661 gccgctcatc tccgtcctca ctgttacggc ctcataaagg gggccttccg ccgat
//
LOCUS       DAOMSU010000209         3703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2456_length_3703_cov_3.136513, whole genome shotgun sequence.
ACCESSION   DAOMSU010000209 DAOMSU010000000
VERSION     DAOMSU010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2456_length_3703_cov_3.136513"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2206)
                     /locus_tag="PK077_08410"
     CDS             complement(<1..>2206)
                     /locus_tag="PK077_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRN24359.1"
                     /translation="PAPPDQQLSFTVKAPGRLTSVKEFENIVVRGQDSSIVRVGDIAR
                     VELGSETYSLSSSVNGMPAASIGIYEAPGGNAIQLVDNIKALLKNTDMPPGMDYLVSL
                     DSTLAVRAGIEDIVSTLVIALGLVVLVVFIFLQGWRGTLIPAFAVPVSIVGAFIAFPL
                     FGFSINTICLMGLVLAIGLVVDDAIVVVEAVKNHISNGEKPLQATIAAMKEVSGPIVS
                     TALVISCVFIPTLLLPGVTGKLFEQFAVTIGMSIIISAFCALSLSPTLSAGLLKGGKA
                     DSIPLLGPFFKLFNKGFNKARDWYVEICARLIRHMWLSILLLAAMTALLFPLAKLIPS
                     GFLPNEDQGYLFGGVELPDNTSLNVTADTAARIEKIIREDPGVDVVTTVNGFNLISSV
                     QSSSNSFFFISLKPWSERTAPGMTADGIAARLKSRLNAEISSGVAYVVPPPPLPGVGT
                     SGDVTFLLEDRQGIGEKFLAANTSKFIEAAEKRPEIAGISNFMSPSNLQYNLNVNTEQ
                     ATLQNMDVDEIYATIQAYMGSTFLNNFNIYGQEWQVYMQADAPYRDSIDKLSMFYVRN
                     NEGDPVPLDSVINVTHGWAPEFLIRQNMFNSSQLNVTPAPGYSSGQVMNALEEVFAQT
                     MPSGMGYDYSGMSYQEKQAQKGITIGMIFAASAVFVFLILASLYESWSLPVAVFMTAP
                     IAILGAFLALWITGLDLNIYSEIGLIVLIALAAKNAILIVEFAVLELRQGTDL"
     assembly_gap    2207..2216
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2217..2878)
                     /locus_tag="PK077_08415"
     CDS             complement(<2217..2878)
                     /locus_tag="PK077_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRN24360.1"
                     /translation="MADFFIKRPILSICLSVIIVLLGAFSILRLPISEYPDIIPPSIQ
                     VTATYPGADCETVVKSIASPIEQQMSGVDGMSYMTSVNTNNGQMSMMILFEIGTEANM
                     DQVLSYLRYGQATSQLPSEVSALGVSLRKTSGLPALVVSLYSPQGTYDGLWLANYAYI
                     NMVDAIKRVPGVGDVQVFGAGRYAMRIWLNPEKMAALNITARDVMLAVQAQNAVNPAG
                     KIG"
     gene            complement(2891..>3703)
                     /locus_tag="PK077_08420"
     CDS             complement(2891..>3703)
                     /locus_tag="PK077_08420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRN24361.1"
                     /translation="QAQLQQLNYDAEIYRPLAAENAISKQKYEDTRLSALAAEAQVQQ
                     AAAALALAEKNLSYTVLYAPFDGIAGISRTNIGDLVSPESGPLAVVSSVNPIRVNFAV
                     TQQEWIQQAGKNGNEGVQTGSKLDITLKDGTKYPEQATVVAIDRAFNPQTGTIRVQAN
                     LPNADYLLRPGMFIIATARLATVKNALTVPAKSLLSTQGRFFVIVLDEGNHPSIIPVQ
                     AGTQLGDRQQVTPLKPDSLTPQSKVVVDGLLQAEAASRNPTARLKAVPASNQ"
BASE COUNT          759 a          981 c         1173 g          780 t
ORIGIN      
        1 gcaggtccgt gccctgacgg agttccagca cggcgaattc cacgatgagg atggcatttt
       61 tggccgccag agcaatcagg acgatgaggc cgatttccga ataaatgttc aggtccagcc
      121 cggttatcca cagggccaga aatgctccca ggatggcaat gggggcagtc atgaagacgg
      181 ctacgggcag ggaccagctc tcatacaggg atgccagaat caaaaagaca aacacggctg
      241 atgccgcaaa gatcatgccg atggtgatgc ctttctgcgc ctgtttttcc tggtagctca
      301 ttccggaata gtcgtatccc atgccggacg gcatggtttg ggcaaaaact tcttccaggg
      361 cgttcatgac ctggccggag gaatagcccg gagccggggt gacgttgagc tgggaactgt
      421 tgaacatatt ctggcggatc aggaattccg gggcccagcc atgcgtaacg ttgattacgg
      481 aatccagggg aaccgggtct ccttcattgt tgcgcacata gaacatggaa agcttgtcga
      541 tgctgtcccg gtacggtgcg tccgcctgca tgtacacctg ccattcctgt ccgtaaatat
      601 tgaagttgtt caggaaggtg cttcccatgt atgcctggat ggtggcgtaa atctcatcca
      661 cgtccatatt ctggagagtg gcctgctccg tgtttacatt caggttgtac tgaaggttgg
      721 agggagacat gaaattgctg atgcccgcta tttccggcct cttttccgcc gcctcgatga
      781 atttggacgt gttggcggcc aggaattttt ccccgatgcc ctggcggtcc tccagcagga
      841 atgtgacatc cccggacgtt cctacgccag gcaggggcgg cggcggaacc acatacgcca
      901 cgccggagga aatttccgcg ttcagcctgc ttttcaggcg tgcggcgatg ccgtccgccg
      961 tcatgcccgg agcagtccgt tcggaccagg gtttcaggga gatgaagaag aaggagttgg
     1021 aggagctttg gacggagctg atgaggttga agccgtttac ggtagtcact acgtccacgc
     1081 ccggatcctc cctgatgatt ttttcaatcc gcgcggcggt gtccgccgtg acgttcaggg
     1141 atgtattgtc cggcagttcc acgccgccga acaggtagcc ctggtcttca ttgggaagga
     1201 atccgctggg aataagttta gccaggggaa agagaagggc ggtcatggcg gccagcagca
     1261 ggatggacag ccacatatgc cggataagtc tggcgcagat ttccacgtac cagtcgcgcg
     1321 ccttgttgaa ccccttgttg aacaatttga aaaagggccc cagcagggga atggagtcag
     1381 cttttccccc tttcagcagc ccggcggaca gggtggggct gagggacagg gcgcagaagg
     1441 cggaaatgat gatggacatg ccgatggtca cggcgaactg ttcaaacagt ttgcctgtga
     1501 cgccgggcag cagcagcgtg gggataaaga cgcaggaaat caccagcgcc gtgctgacga
     1561 tggggccgga tacttccttc atggccgcaa tggtggcctg gaggggcttt tctccgttgg
     1621 aaatatggtt cttcaccgct tccaccacca caatggcatc gtccacgacc agaccgatgg
     1681 ccagcaccag ccccatcagg cagatggtat tgatggaaaa gccgaacaat ggaaaggcga
     1741 tgaaagcgcc cacgatagac accggaacgg caaaggccgg aatcagcgtg ccgcgccagc
     1801 cctggaggaa aatgaaaacc accagcacca ccaggcccag ggcaatcacc agcgtgctga
     1861 cgatgtcttc aatcccggcg cggacggcca gcgtggagtc cagggaaacg aggtaatcca
     1921 tgccgggcgg catatccgta tttttcagca gggctttgat attgtccacc agctggatgg
     1981 catttcctcc gggcgcctcg taaatgccga tggaagcggc gggcatgccg tttacggaag
     2041 aactgaggct gtaagtttcc gagcccagtt ccacgcgggc gatatctccc actcttacga
     2101 tggaactgtc ctgcccgcgc accacgatat tttcgaattc cttcacgctg gtcaggcgtc
     2161 cgggcgcttt tacggtgaag gaaagctgct ggtccggcgg ggcgggnnnn nnnnnnccta
     2221 tcttgcccgc aggattcacg gcgttctggg cctgaacggc cagcatcacg tcacgggccg
     2281 taatattcag ggcggccatc ttttccgggt tcagccagat gcgcatggcg tagcggcctg
     2341 ccccgaacac ctgcacgtct cctactccgg gcacgcgctt gatggcgtcc accatattga
     2401 tataggcgta attggccagc cagaggccgt cataagtccc ctggggggaa tagaggctga
     2461 cgaccagggc gggcagcccg cttgtcttgc ggagggagac tcccagcgcg ctgacttccg
     2521 acggcagctg ggaggtggcc tgcccgtagc gcaggtagga aagcacctgg tccatgttgg
     2581 cttccgtgcc gatttcaaaa aggatcatca tgctcatttg cccgttgttg gtgttgacgg
     2641 aagtcatgta ggacatgccg tccacgccgg acatttgctg ttcgatgggg gatgcgatag
     2701 acttgaccac cgtttcacag tccgctccgg gataagtggc cgtcacctgg atggaggggg
     2761 gaatgatgtc cgggtactcg gagatgggca ggcgcagaat ggaaaaggcc cccagcagga
     2821 caatgatgac ggagaggcag atggaaagga tggggcgctt aatgaagaaa tcagccatgg
     2881 cgggaggagc ttattggttg ctggcgggta ccgctttcag gcgggcggtc ggatttctgg
     2941 aggctgcttc cgcctggaga agaccgtcca cgaccacttt gctttggggg gtgagggaat
     3001 ccggtttcag gggagtaacc tgctgccggt ctccaagttg cgttccggct tgtacgggga
     3061 tgatggaagg gtggttgccc tcatccagaa caatcacgaa aaagcgcccc tgtgtcgaaa
     3121 gaagggattt tgccggaacg gtcagcgcgt ttttgacggt ggccagccgg gccgtggcga
     3181 tgatgaacat gccggggcgc agcagatagt ccgcattggg aaggttggcc tgtacgcgga
     3241 tggtgcctgt ctggggattg aacgcccggt caatggcaac taccgtggct tgttccgggt
     3301 atttggtgcc gtctttcagc gtaatgtcca gcttgctgcc cgtctggacg ccttcattcc
     3361 cgtttttccc cgcctgctga atccattcct gctgggtgac ggcgaaattg acgcgtatgg
     3421 gattgacgga ggagacgacg gcaagggggc cgctttccgg gctgaccagg tcgccgatgt
     3481 ttgtcctgga aatgccggct attccgtcaa aaggcgcgta cagcaccgta taggaaagat
     3541 ttttttcagc cagggccagg gccgccgcag cctgctgcac ctgcgcttcc gcggccaggg
     3601 cggagaggcg cgtatcctcg tatttctgtt tggaaatggc gttctccgcg gccaggggcc
     3661 tgtaaatttc cgcgtcgtaa ttcaactgct gtagctgggc ctg
//
LOCUS       DAOMSU010000210         3685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2477_length_3685_cov_2.472176, whole genome shotgun sequence.
ACCESSION   DAOMSU010000210 DAOMSU010000000
VERSION     DAOMSU010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2477_length_3685_cov_2.472176"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            217..825
                     /locus_tag="PK077_08425"
     CDS             217..825
                     /locus_tag="PK077_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24362.1"
                     /translation="MKITSFIIRTLCAGTVGSLACQAAMQAYADDVPSTFVEGNYSPE
                     VARYVQSIAGVALASGIHDDDENATSSYQVLAQVLNSGNVTELPEDLKRAIGMLTGYV
                     PNPNETIQPIMSRKQIERMRKMADRIKNLMEEKYGVDLEDCTNIMKRTLAPSIPDILR
                     SCGMEGTQLPATTAFRPRLGKALNEAYARLAAPKLPAAGMAQ"
     assembly_gap    1247..1256
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1324..>2411
                     /locus_tag="PK077_08430"
     CDS             1324..>2411
                     /locus_tag="PK077_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRN24363.1"
                     /translation="MDNSSSRRRFLQTLGLATGALAAGSFANAQEVAPLAPKKITIPD
                     PNNIGPMTTWPPRKPGAIYMGGFRAPKLDKVRVAFVGVGERGSMHVGQMAVIEGAEIV
                     GICDLYEDWAKRSADVVEKKTGKRPPIFTKGPEDYKRMMKEVKPDAVIVCPSWEWHCR
                     VTCDVMKMGAHAFVEVPMAVSIKELWEIVDTSEETRKHCMMMENVNYGREELMYLNMV
                     RQGVIGDLLYGEAAYIHELRGQMKQAERGTGSWRTYHYAKRNGNVYPTHGLGPIAQYM
                     NLARKDDCFGRLVSFSSPALGRAAYAKKNFPADHKWNKLDFACGDMNTSIIKTTMGRT
                     VLVEWDETSPRPYSRLNLIQGTLGTLDKV"
     assembly_gap    2412..2421
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2422..2780
                     /locus_tag="PK077_08435"
     CDS             <2422..2780
                     /locus_tag="PK077_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-N-acetylgalactosaminidase"
                     /protein_id="HRN24364.1"
                     /translation="PTRVAGEKLGNGNYHEWIEGKEKLAPIFEKYDHPLWKRIGPLAL
                     KMGGHGGMDFVMLFRIIECLRNGEPMDQNVYEGAFWSSVSELSEYSVAQGGMPQVFPD
                     FTRGDWKTTAPLGIVQ"
     gene            3082..>3685
                     /locus_tag="PK077_08440"
     CDS             3082..>3685
                     /locus_tag="PK077_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24365.1"
                     /translation="MLSYCTNIHPAESWAETREALFTCVPRIRQELAAMDSPLKDLPL
                     GIGLRLSARAAAELLETPHAVETLKSWLEDQGARVETLNGFPYGNFHGQRVKERVFQP
                     DWTTPERFEYTCNLFRILALIGDEQADRLTVSTLPASHSWFHADEERIFSRLDAMSGF
                     LDVLGRQTGRLMQLGLEPEPFGHFHDTDGAIRFFNGLRNRS"
BASE COUNT          838 a         1126 c          983 g          718 t
ORIGIN      
        1 cggtttttcc atttcccgtc aataaccgga accggcgaaa cggacgcatt ctccctgctc
       61 cttattcaac aacttttaaa cggtccggaa agcgcggaag catgatttta atcattcaaa
      121 aaacttttga acggaattga agtcagcgcg gtctcacttg ttgcaaacgg ttcgccggac
      181 cgttccagtc gggaagcttc aaccggagtt tcaatcatga aaataacctc gttcatcatc
      241 aggacactat gcgcgggcac cgtcggttcc ctcgcctgcc aggccgccat gcaggcttac
      301 gcggatgacg ttccctccac cttcgtggaa ggaaattatt ccccggaagt agcccgctac
      361 gtgcagagca tcgcaggcgt ggcgctggcc tcgggcattc atgatgatga cgaaaacgcc
      421 accagcagtt atcaggttct ggctcaggtc cttaatagcg gcaatgtaac ggaattgccg
      481 gaagatctga aacgggccat tggaatgctg actggctacg tccccaaccc gaatgaaacc
      541 atccagccga tcatgtccag aaaacagatt gagaggatga gaaagatggc agaccgcatc
      601 aagaacctga tggaggaaaa atacggagtg gacctggaag actgcacgaa catcatgaag
      661 cgcactctgg ccccgtccat cccggacatc ctccgctcct gcggcatgga aggcacccag
      721 ctgcctgcca ccaccgcttt ccgccccagg ctgggcaagg ctctgaatga agcctatgcc
      781 aggcttgcgg cgccaaaact tcccgccgcc ggcatggcgc aataaccgga ccatctacgg
      841 atgatcggga agataatccc gattccacgg cacgcggccc cctgccaggg tcccctgaat
      901 agaagccgca ggaaacggaa tgatcttccc cttctccggg aacggaaacg gcagccagcg
      961 tcggcggcaa caagcaacac cctccggatg tcctccgatg cagggccgat ccttcggaac
     1021 gcggagcggc gatacgcccg ctcccctgtt ttattcccaa gcaacggcat gcaacatcgg
     1081 cacaaccgga tgatgccctt acaggccccg tccttcccgc gctgcggcag gaccggggct
     1141 ttttcatatc cgggattttc aaaaggcggc ggcataccct cctgattctc ttcaggaatc
     1201 agggatattc cgtttttaca gaggaatgca ttttttcatc atctgtnnnn nnnnnngaaa
     1261 gcttctcaag cctcagtatt tatggcagac tagggattca aatccttcat acaattacca
     1321 tccatggata attcatcatc acgccgtcgt ttccttcaga ccctgggcct ggccactggc
     1381 gcccttgctg ccggttcctt tgccaacgcc caggaagtag cccccctggc tcccaagaaa
     1441 atcaccattc cggacccgaa taacatcggc cccatgacca cgtggccccc gcgcaagccc
     1501 ggcgccatct acatgggcgg cttcagggct cccaagctgg acaaggtgcg tgtggccttt
     1561 gtcggcgtgg gtgaacgcgg ttccatgcac gtgggccaga tggccgttat agaaggtgcg
     1621 gaaattgtcg gcatttgcga cctgtatgaa gactgggcca agcgcagtgc ggacgtcgtg
     1681 gaaaagaaga cgggcaagcg cccccccatt ttcacgaaag ggccggaaga ctacaagcgc
     1741 atgatgaagg aagtcaagcc ggacgccgtc atcgtctgcc ccagctggga atggcactgc
     1801 cgtgttacct gcgacgtgat gaagatgggc gcccacgcct ttgtggaagt gcctatggcc
     1861 gtctccatca aggaactctg ggaaatcgtg gatacctccg aagaaaccag gaagcactgc
     1921 atgatgatgg aaaacgtcaa ctacggacgt gaggaactca tgtacctgaa catggtgcgc
     1981 cagggcgtca ttggcgacct gctgtacgga gaagccgcct acatccatga actgcgcgga
     2041 cagatgaagc aggcggaacg cggcaccggt tcctggagaa cctatcacta cgccaagcgc
     2101 aacggcaacg tgtatcccac gcacggcctc ggccccattg cccagtacat gaatctggcc
     2161 cgcaaggacg actgcttcgg caggctcgtc tccttctcca gcccggccct gggccgcgcc
     2221 gcgtatgcca agaaaaattt cccggcggac cacaagtgga acaagctgga ctttgcctgc
     2281 ggcgatatga atacttccat catcaagacc accatgggcc gcaccgtcct ggtggaatgg
     2341 gatgaaacca gtccgcgccc ctactcccgc ctgaacctta tccagggcac cctgggcacc
     2401 ctggataagg tnnnnnnnnn ntcccgaccc gcgtagccgg ggaaaagctg ggcaacggaa
     2461 attatcatga atggattgaa ggcaaagaaa aactggcccc catttttgaa aagtacgatc
     2521 acccgctctg gaagagaatc gggccgctgg ccctgaagat gggcggtcac ggcggcatgg
     2581 acttcgtgat gctcttccgc atcatcgaat gcctccgcaa tggcgaaccg atggaccaga
     2641 acgtttatga aggtgccttc tggtcctccg tctccgagct ttccgaatac tccgtggccc
     2701 agggcggcat gccccaggta ttcccggact tcacccgcgg agactggaaa acgactgctc
     2761 cgctgggcat cgtccagtaa cagccggcaa ccttccccgg cccgaagctt ttgcgcctcc
     2821 atggcgtgtt tccgggccgg ggcaaaatgc ctcctcttcc aaaaacaggc ccagcttgca
     2881 ggcggcctgt tttttattgc tccaatgaca ccctattccg gaagttgaaa ggctccgcag
     2941 gcactccact tgccggggaa agctcccgga tgacgccaaa aagcggaact ccatgaaatt
     3001 ttccttctgc gcggaaccgt atttagacaa gaaaaacatc ctgcggcaaa acgccgttcc
     3061 ccttacatcg cacacgcctt catgctttcc tattgtacta atattcatcc cgcagaatcc
     3121 tgggcggaaa ccagggaggc tcttttcacc tgcgttcccc gcatcaggca ggaactcgcg
     3181 gcgatggact cccctctgaa ggatctcccc ctgggcatcg gcctgcgcct gtccgccagg
     3241 gcggcagcgg agcttctgga aacgccgcac gccgtggaaa ccctgaaatc atggctggaa
     3301 gaccagggcg cgcgcgtaga aacccttaac gggttccctt acggaaattt ccacgggcag
     3361 cgcgtgaaag aacgcgtttt ccagccggac tggactacgc cggaacgttt tgaatacacc
     3421 tgcaacctgt tccgcattct ggcactcatt ggagacgaac aggctgacag gctgaccgtc
     3481 agcacgctcc ctgcctcgca cagctggttt catgcggatg aagaacgcat cttctcccgg
     3541 ctggacgcca tgagcggatt cctggatgtg ctgggcaggc agaccggccg actgatgcag
     3601 ctggggctgg aaccggaacc cttcggccat tttcacgata cggatggagc catccgtttt
     3661 ttcaacggcc tccgcaaccg ttccc
//
LOCUS       DAOMSU010000211         3659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2505_length_3659_cov_2.680633, whole genome shotgun sequence.
ACCESSION   DAOMSU010000211 DAOMSU010000000
VERSION     DAOMSU010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2505_length_3659_cov_2.680633"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1405
                     /locus_tag="PK077_08445"
     CDS             194..1405
                     /locus_tag="PK077_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24366.1"
                     /translation="MTVPVKILSVLAGLSLAGSARARIWTNDRGATVVAVLVAVRDAE
                     VDLKLQDGRVVAVPKNIFSGADQEYILEWVKSGGTLPDTDVGAENPPVQGDSGRRYVP
                     LEPNWDAPWPERVVVPGFLLVKTVQETEELSVYETDHFIMESPGKLLEAERMILARRF
                     ETILSALAAVPLNLAVARRPSRKYLVRVCFQEEDFNRAPGLRNGHLKFSPTSFTALLL
                     RNKKGKLLKPELDPRFAVTHWAAQSMDWEHWLVDGFSAYMAFLPMEKEAPVFRKIPER
                     LAAMVPRAVRTGRETLPALADMLSRDSAHSAAEHGVSSRGGTLQYWADLLWMVYWSHL
                     EGNGKAERLRSYLRVRDAEGGGKARAVLLDGKTPEDVQGEMAAAWKKMGLRLRFSQPA
                     SAAADGKSAEK"
     gene            complement(1459..>3659)
                     /locus_tag="PK077_08450"
     CDS             complement(1459..>3659)
                     /locus_tag="PK077_08450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24367.1"
                     /translation="MNLSLQPTASSVILTVLVMAAGIWMSYIACKRNRRSLTFKLEIL
                     RLAILGFICFLLFQPEWLITSSPQEKPKVSILEDRSGSMETQDVEISPQHVVTRTAYV
                     QELLKGNSTESLKDTHVVEYASFGAPPAPDSPDYAMAGTDLAKPLEDAMQSSDNLRAV
                     IMFTDGSHNASSSVLTQAQRMRTRGIPLFIISAGSPYPLPDLALQDVKAPTYGIIGET
                     VQIPFTIKSTLGKETRATLTMISRDTGKTVTRTVTIPAQGEVSDAVLWKIEKEGAETL
                     ELKLPAQPQERMHNNNASSFSISGRRESIKALVIDTLPRWEYRFIRNALYRDPGVNVH
                     TLLFHPELEEMGEGPGYLVKFPDRMEDLARYDVIFIGDVGLGSKGLTEEQASLLKGMV
                     KNQASGIVFLPGYQGRQMELLKSELGDLMPVTFLNTKPEGTTQPSPSPLILTPEGRGS
                     LLTMLADTEGENEEVWRNLPGFNWYAPVERTKAGSTVLAVHAADKNAYGRIPLIVTQS
                     YGHGKVLFMGTDSAWRWRRGVEDKYHYRFWSQVARWMSYQRNMAAGERIRLIPTPERP
                     RPGDTLTVTAMVSDKLGAPLQNGEVFLDITTPEGSSSRVQMDTMNHTWGSFTASVKIN
                     RPGTWKLTASSSAEPDKSVTLPIITMSETLEKIGSPVNTALMEEMASITRGRMVKAEE
                     IQRLIKEIRDLPVPPPMETRILLWCSPYTLSILILLLTLFWIGRKLNGTI"
BASE COUNT          682 a          918 c         1209 g          850 t
ORIGIN      
        1 atgcatgctt tcttttcctt ccttccctaa gattatgggt tttgccggag cgtgtcaacc
       61 cctccggggg acaggctgcg gaggattcct ttcccatatt cttttcaagg aacaggttcc
      121 ggaagagagg gggatggaaa aagggttgcg tctgcgggac aatgtgatat cttgtgctgg
      181 caaagggaac cctatgaccg tacctgtaaa gatactgtcc gtattggcgg ggctgagcct
      241 ggctggaagc gcccgggcgc gtatttggac gaatgaccgg ggcgctaccg tcgtagccgt
      301 gctggtggcc gtccgtgatg cggaggtgga tttgaagctt caggatggtc gcgtggtggc
      361 tgttcccaag aatatttttt ccggggccga ccaggaatat attctggaat gggtaaagtc
      421 tggcgggacg ctccctgata cggatgtggg ggctgagaat cctcctgtgc agggggattc
      481 cggcagacgg tacgttcccc tggagccgaa ttgggatgct ccctggccgg agagggtagt
      541 tgttcccggt ttcctgctgg tgaaaaccgt gcaggagacg gaggagctga gtgtgtatga
      601 aacggatcat ttcatcatgg agtctcccgg aaagctgctg gaagcggagc gtatgatcct
      661 tgcccggcgt tttgaaacca ttctgtctgc cctggcagct gttccgttga atctggcggt
      721 ggcccgccgg ccttcccgca agtatctggt gagggtatgt ttccaggaag aggatttcaa
      781 cagggctccg ggcctgcgga acgggcatct caagttttcc cctaccagtt tcacggctct
      841 gctgttgcgg aataagaagg gaaagctgct gaagccggaa ctggatccgc gctttgccgt
      901 cacgcactgg gcggcgcagt ccatggattg ggagcactgg ctggtggacg gcttttccgc
      961 gtacatggct tttcttccca tggagaagga agcgcctgta ttccggaaga ttccggaaag
     1021 gctggccgca atggttccgc gcgccgtccg cacgggcaga gaaactctgc cggccctggc
     1081 ggacatgctg tccagggatt cagcacattc cgccgcggag catggcgttt cctccagggg
     1141 aggaacgctt cagtattggg ccgatttgtt gtggatggtt tactggagcc acctggaagg
     1201 gaacggaaag gcggagcgcc tcagaagcta tttgagggtt cgtgatgcgg agggcggcgg
     1261 gaaggcgcgt gctgttttgc tggatgggaa gactccggag gatgtacagg gggaaatggc
     1321 cgcggcctgg aaaaaaatgg gattgaggct gagattttct cagccggctt ctgctgccgc
     1381 ggacgggaag tccgcggaaa aataacggga agggagggaa gaaaaactcc tgccggggag
     1441 gtgtgggaaa cctgcgtctc aaatggtgcc gttgagtttt ctccctatcc agaacagggt
     1501 aagcagaagg atgaggatgg agagcgtata cgggctgcac cagagcagga tgcgcgtttc
     1561 catgggcggc ggcaccggga gatccctgat ttccttaatg agccgctgta tttcctcagc
     1621 ttttaccatg cgcccgcgtg tgatggaggc catttcctcc atgagcgcgg tattgacggg
     1681 gctgccgatt ttttccagcg tttcactcat ggtgatgatg ggaagcgtaa cggatttatc
     1741 cggttccgca gaggatgaag ctgtgagctt ccaggtgccg ggacggttga ttttgacgga
     1801 ggcggtaaag gagccccatg tatggttcat cgtgtccatt tgcacgcggc tggagcttcc
     1861 ttccggagtt gtaatgtcca gaaaaacctc tccattctgg agaggggccc ccagtttgtc
     1921 ggagaccatg gcggtgacgg tcagcgtgtc tcccggccgg ggccgttccg gggtgggaat
     1981 cagacggatt ctttctcctg cggccatatt gcgctggtaa ctcatccagc gggccacctg
     2041 gctccagaaa cggtaatggt atttatcttc tactccgcgc ctccatcgcc aggcggaatc
     2101 cgttcccatg aagaggactt tcccgtggcc gtaagactgg gtgacaataa ggggaatgcg
     2161 gccataggca ttcttatccg ccgcatgcac ggccaggaca gtgcttcccg ctttggtccg
     2221 ttccacgggg gcgtaccagt tgaagcccgg cagattgcgc catacttctt cgttttcgcc
     2281 ctccgtatcc gccagcatgg taagcaggga gccgcggcct tccggcgtca ggataagggg
     2341 tgatggggaa ggttgggttg tcccctccgg tttggtgttg agaaatgtaa cgggcatcag
     2401 gtcccccagt tccgatttca ggagttccat ttgcctgccc tggtagccgg ggaggaagac
     2461 gatgccgctg gcctggtttt ttaccatgcc tttgagcagg gaggcctgtt cttccgtcag
     2521 ccctttggag cccagcccca catcgccgat gaagatgacg tcataccggg ccaggtcttc
     2581 cattctgtcc gggaatttga ccaggtatcc ggggccttcc cccatttctt ccagttccgg
     2641 atgaaagagc agggtgtgca cgttcacgcc gggatcccgg tacagggcgt tccggatgaa
     2701 gcggtattcc cagcgcggca gtgtatcgat gaccagtgct ttgatggatt ccctcctgcc
     2761 ggagatgctg aaggaagagg cgttattatt gtgcatccgt tcctggggct gggcggggag
     2821 tttgagttcc agcgtttccg ccccttcttt ttctattttc cacagaacgg cgtcggatac
     2881 ctctccctgg gccggaatgg tgaccgtgcg ggtgacggtt ttccctgtgt ccctggaaat
     2941 catggtgagc gtggcgcgcg tttccttgcc cagcgtgctt ttaatggtaa agggtatttg
     3001 caccgtttcc ccgatgatgc cgtaagtggg ggctttcacg tcctgaaggg ccaggtccgg
     3061 gaggggatac gggctgcctg cggaaatgat gaacaggggg attcccctgg tgcgcatgcg
     3121 ctgggcctgc gtcagcacgg aggatgaggc gttgtggctg ccgtccgtga acatgatgac
     3181 cgcccggagg ttgtcggagg attgcatggc atcctccagc ggtttggcca ggtctgtgcc
     3241 ggccatggca taatccggag agtccggcgc aggtggcgcg ccgaaggagg cgtattccac
     3301 tacatgcgtg tctttgaggg attccgtact gttgcccttg agcagttcct gcacgtaggc
     3361 cgtgcgtgtg acgacatgct ggggggagat ttccacatcc tgcgtttcca tggagccgga
     3421 gcggtcctcc agaatggaaa ccttgggttt ttcctgcggg gaggaggtga tgagccattc
     3481 cggctggaag agcaggaagc agatgaagcc aaggattgcc aggcgaagaa tttccagttt
     3541 gaatgtcagc gaccggcggt tgcgcttgca ggcgatatag ctcatccaga tgccggccgc
     3601 cataaccagg acggtcagga tgacgcttga cgcagtaggt tgaaggctca ggttcatgg
//
LOCUS       DAOMSU010000212         3658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2506_length_3658_cov_3.003331, whole genome shotgun sequence.
ACCESSION   DAOMSU010000212 DAOMSU010000000
VERSION     DAOMSU010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2506_length_3658_cov_3.003331"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1263..2033
                     /locus_tag="PK077_08455"
     CDS             1263..2033
                     /locus_tag="PK077_08455"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN24368.1"
                     /translation="MNTWNTLINSTPGRTLNVGLTWYDGADSSTLASAYSPYYYYLSK
                     KPQQVSTMAEAVWRDGSTRTTSGYDIYIQCNTSRLASLYYGAALLAEHTGKYDFQSIL
                     THEVGHAVGFLSLATQTGTFQVQSGSASTTYSTMLYTKYDSLLTNQEGQSIVEKAGNG
                     NTAFTLGETLSLGDTGLTVYNPTTWSEGSSMAHIDSTSDPDALMQYSISPDTYHRTLT
                     DGEVGLMRSMGWNMVPEPATATLSLLGLAALALRRRRC"
     gene            complement(2168..>3658)
                     /locus_tag="PK077_08460"
     CDS             complement(2168..>3658)
                     /locus_tag="PK077_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRN24369.1"
                     /translation="TDAAIVAAATLSDRYISDRFLPDKAVDLVDEAAARLKIELDSMP
                     TEIDQIEREAMQLEMERQALAKEEDADSKARLEKITKDLADLKEKSGSMIAKWKSEKE
                     VLDAVRREQEKIEALKLESERAKRMGDLTRASEITYGELPEAQRALTEGKEKLSKMQK
                     EDGGLLKEEVTEGDIAKVVATWTGIPAARLQEGERAKLVHMEERLGARVIGQKQAVKA
                     VSDAVRRARAGLQDENRPIGSFLFLGPTGVGKTELSKALAEFLFDSENAIVRIDMSEY
                     MEKHSVARLIGAPPGYVGYEEGGQLSEAVRRRPYCVVLFDEIEKAHPDVFNVLLQVLD
                     DGRITDGQGRTVDFRNTVIIMTSNIGSQYILNEANAEQREAKALEALRGHFRPEFLNR
                     IDEIIIFDRLTAQELKGIVDIQLQRVRRRLEAKGLFLRMTPEATALVADHGFDPVYGA
                     RPLKRAIQHDLLDPLSLKLLEGDFPEGTEIVVREKDGKLDFTKEKR"
BASE COUNT          798 a         1015 c          909 g          936 t
ORIGIN      
        1 gccgagaaat ctaccatacc acaccttttt gtcaattttt ttcgagatgt tttttcattt
       61 tttcccgatc cttattcagg ccatacccac tgcaaccacc cttaatacac acctaatcaa
      121 tgagatgctc ttttgtcata atcccccatc ctggcgcgtc tttttttgaa cgcttccctt
      181 tttcccctcg ctcttcccct ttttgaaaag ggacagtata gttgaaatag ggtgaaagct
      241 agcaatggcg ggaaattaca gcgatggaaa gaagagacgg gtaattgtga tgaaggaaca
      301 tgaaactgcc tgaaacggga caggttgttg cgatatgaaa tcgcccctct tcggaggggc
      361 tgtcaatatt acgaggccaa agccaagccg aaaataatgg ttttgaggga aagatgtcga
      421 ccgaaccgga ggacacccgg gaatggtaaa caaaaagatt ccggaacgat cggtatttcg
      481 gagaattccc gctggtcctg ccggtgctgg cacagcgccg aggctccccc tgatttatac
      541 gcccggatgt tccggatggt gacgacaggc aatccccggc cttatggagt gccgcgcgag
      601 cgcagggcgg aaattatgca ctccctttgc aacgccaaaa taaagctgat ggcagcggcg
      661 gcaatagccc cggttcaagg tacggatcaa ccttccttcc ggtgagtttt ttatagcaaa
      721 ttcgggggta aaagtcacgc gaattcaccc gttttcaggc gtttttttaa cctcttttca
      781 ggcttccgga aaggggcttt tcctggcagc aagtggcgaa atatcgcaac aacctgtccc
      841 gcatatcgca actatcttgc gccgttacat tttttacaag gaaactgtcc ttaccaggag
      901 ggcccctgcc ggtattttca gcattttttg ttgttatgca acaagagcca gaaaaaaaga
      961 aggggtgtct gacccgtctt ccctgaaaaa gaagcagact tgatttattt tcacttgaac
     1021 agaaaggatt tttttgctat tcaagggttt attatttttt atctatgaaa agtccattcc
     1081 aaattggcat tctctgcact gttttctgct tgggaagcac ttttttctca tctgctgtac
     1141 agacagttaa tataggctcc actttttcat gcaccttcct agattccggg gagaagggat
     1201 tttttgattc ttcaagtact catacctgga ccagtgatca gatagattcc gccatacggg
     1261 cgttgaacac atggaatacc ctgattaatt ccaccccggg aagaacgctt aacgtaggcc
     1321 tcacctggta cgacggagcc gattcctcca ccctagcctc cgcttattcc ccttattact
     1381 actacctgtc aaaaaagcca caacaggttt ccaccatggc cgaggccgta tggcgcgatg
     1441 gaagcacaag gactacctca ggctatgata tttacatcca gtgcaatacc tctcgtttgg
     1501 cttcccttta ttatggggcc gccttattgg cggagcatac gggaaaatat gattttcaaa
     1561 gcatcctgac ccatgaagtg gggcatgccg ttggttttct ttccctggcg acgcagacag
     1621 gcactttcca agtccaatcc ggaagcgctt ccacaaccta cagcaccatg ctgtacacca
     1681 agtatgattc cctgttaacc aaccaagaag gccagtccat cgtagaaaaa gccggaaatg
     1741 ggaatacggc atttacccta ggggaaactt tgtctctggg cgatacgggg cttactgtct
     1801 ataaccccac cacatggtcg gaagggtcca gcatggccca cattgacagc acgagcgatc
     1861 cagacgccct tatgcagtat tccatctcac cggataccta tcatcgcacc ctgacggatg
     1921 gagaagtggg attaatgcgt tccatgggct ggaacatggt tccggaaccc gctaccgcta
     1981 cgctttccct cctagggctg gccgcactcg ctttgcgccg ccgccgttgc tgacgacgct
     2041 ctgtcccaga tttaaccgcg cagcagattt tcttcagctc ccggaagcgg gaatctttat
     2101 atcagaaggg cggccctctc aggggacgcc cttcattgtt tttccggcaa catgccgaga
     2161 aatgcaatta tcttttctcc ttggtgaagt ccagtttgcc gtctttttcc cgtaccacga
     2221 tttccgttcc ttccgggaag tctccttcca gaagcttgag gctgagcggg tccagaagat
     2281 catgctgaat ggcccgtttg agaggacggg ctccatacac aggatcaaaa ccatgatccg
     2341 ccaccagagc cgtagcttcc ggagtcatgc gcaaaaacag gcccttggct tccaggcgcc
     2401 tgcggacacg ctgaagctga atgtccacaa tgcccttgag ttcctgcgcc gtgagccggt
     2461 caaagatgat gatttcatca atgcggttca ggaattccgg acggaagtga ccgcgcaacg
     2521 cttccagggc cttggcttcc ctctgttccg cattggcttc attgaggatg tactggctgc
     2581 cgatgttgga ggtcatgatg atgaccgtgt tgcggaagtc aaccgtacga ccctggccgt
     2641 ccgtaatgcg gccgtcgtcc agcacctgga gcaatacgtt gaagacgtcc gggtgggcct
     2701 tttcaatttc atcgaagaga accacgcagt acggacgacg gcgcacggct tcagagagct
     2761 gaccgccttc ttcatacccc acgtatccgg gaggcgcccc gatgaggcgc gcgacggaat
     2821 gtttttccat gtattcggac atgtcaatgc gaacgatggc gttctcactg tcaaagagga
     2881 attccgccag cgccttgctg agttccgtct tgcccacgcc ggtgggtccc aggaacagga
     2941 aagagccgat gggcctgttt tcatcctgca gcccggcgcg ggcgcgtctc acggcgtcgg
     3001 atacggcctt aacggcctgt ttctgcccga ttacgcgggc gcccagacgc tcttccatgt
     3061 gcaccagctt ggcacgctcc ccttcctgca gacgggcggc gggaatgccg gtccaggtgg
     3121 caaccacttt ggcaatgtct ccttccgtga cttcctcctt cagcagccct ccatcttctt
     3181 tctgcatttt actgagcttt tccttgcctt ccgtcagggc gcgctgggct tccggaagtt
     3241 cgccataggt gatttccgac gcgcgggtca ggtcacccat acgtttggcc ctttcgcttt
     3301 ccagcttgag ggcttctatt ttttcctgtt ccctgcggac ggcgtccagc acttcctttt
     3361 cgcttttcca tttggcaatc atggaaccgg atttttcctt caggtcggcc aggtccttgg
     3421 tgattttttc caaacgggcc ttgctgtcgg cgtcttcttc cttcgccagc gcctgccgtt
     3481 ccatttcaag ctgcatggcc tcacgttcaa tctggtcaat ttccgtgggc atggagtcca
     3541 gctcaatttt gaggcgggct gccgcttcat ccaccaggtc tactgccttg tcaggcagaa
     3601 aacggtcgga aatataacgg tcggaaaggg tggccgccgc tacgatggca gcgtccgt
//
LOCUS       DAOMSU010000213         3658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2507_length_3658_cov_2.199833, whole genome shotgun sequence.
ACCESSION   DAOMSU010000213 DAOMSU010000000
VERSION     DAOMSU010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3658
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2507_length_3658_cov_2.199833"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="PK077_08465"
     CDS             <1..305
                     /locus_tag="PK077_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HRN24370.1"
                     /translation="IRFIDAELRRRMPKIKAIMPDASYLVFLDCRELNLSQKELVEFF
                     VDGARLALNDGSIFGKEGEGFMRLNAGCPRSILERALSQLEEAYENRGFRQQQSQN"
     gene            533..2185
                     /gene="metG"
                     /locus_tag="PK077_08470"
     CDS             533..2185
                     /gene="metG"
                     /locus_tag="PK077_08470"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRN24371.1"
                     /translation="MTLLAKRQERSIVPRMYYITTAIDYTNGPPHIGHAYEKVLADVL
                     ARWHRMKGEEVYFLTGVDQHGQKVQQTAEKLGITPQEHVNNITGQFLALWDRLGISND
                     GWASTTDPRHKACVQKILTDLKDKGQLYKKSYKGFYSVRQEQFLTDKERDAEGNFGPE
                     WGEVVELEEENWYFRLTDHVEWMKQFVEKSSDFVLPSFRKAEVLNAIDFDSDLCISRP
                     KSRLSWGIEFPFDPEFVTYVWFDALINYISFAGYLAEPDSGLPDFAKLWPADCEVIGK
                     DILVPAHGVYWPAMLHAMGFSDEEMPTLLVHGWWNINGEKMSKSIGNVVDPNLLAEQF
                     GPEPVRYYLVRDITTGKDANFDADRLVMLYNTELANDLGNLCNRSINMTRRYCDSVIS
                     SAEAYDDEASKALRATMDQAVTLFSKYMDDNMASDALAALNAQVSACNAYIEQQQPWQ
                     LAKDEASAPRLRCVLRHLLECCAQTGYLIGCVLPDASARILGQLNAGDLFRSLTPAAL
                     EWGILPAGHRINDPAPVFPRILSEEEKAKLAEKAARAAKKQG"
     gene            2191..>3658
                     /gene="menD"
                     /locus_tag="PK077_08475"
     CDS             2191..>3658
                     /gene="menD"
                     /locus_tag="PK077_08475"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HRN24372.1"
                     /translation="MNSSASFVKSLLAQCCLGGICEWVVCPGARNMALLQVLAAAEDL
                     VKWTHFDERSAAFFALGRIQDMGLPVAVVTTSGTAAAELLPAVVEAYYQRRPLLLLTA
                     DRPAACRGSAAPQAIEQVDLFGIYAPTIDLETPESLPEDILQDWDYASPLHINVCLPD
                     PDPAWNPGSCDLYPAEPPEENGFRGSLAELARALRFKSRDGLVVMIGGLDPTEQAPAR
                     WLANELKAPVVADATSGLREELAHLALTDADALLREHPPAVLLRLGDVPVGRFWRDLE
                     DIPATEVFSVTRTGFSGLARPSSVVTGDLEAILHALGDIDTVGDVNGLRAMNKRRKAL
                     MEELLITCPESEQAMVRSFSCFAADGDCIYLGNSMPVRYWNSFAQTSIPTENVRANRG
                     ANGIDGQISGFLGVSARCSRSWALVGDLTAMYDSNALALLPQLDRGTRVLGVINNGGG
                     GIFRTLPGADGQPETMRKLLVQPHAHSFKAIAEQWGMRY"
BASE COUNT          736 a         1203 c         1059 g          660 t
ORIGIN      
        1 acatccgctt cattgacgcg gaactgcgcc gccgcatgcc caaaatcaag gccatcatgc
       61 cggatgcctc ctacctggtc tttctggact gccgggaact gaacctttcc cagaaggaac
      121 tggtggaatt ttttgtggac ggagcgcgcc tggctctcaa cgacggttcc atcttcggca
      181 aggaagggga aggcttcatg cgcctgaatg cgggatgtcc ccgctcgatt ctggaacggg
      241 cgctcagcca gctggaggaa gcctatgaaa accgcggctt ccggcaacaa caatcacaaa
      301 attaaagaca cgcttcacgc tccccggcaa tggagttcat ttccggaacc atgcattggg
      361 ggaattttcc aggggacatc cggccccgcc gtattttctc ctgttgagcc ccttgcggag
      421 aaggctgcgg cgcggtggaa agggaatatc cgcacaaccc cttccgccgt ttcgcccgcg
      481 acaccccctt actctcccat cccatgctga aaaacacgcc cgggatgccg ccatgacttt
      541 gcttgcaaaa cggcaggaaa ggagtatcgt cccccgcatg tactatatca ccacggccat
      601 tgactacacc aacggaccgc cccacatcgg gcacgcttat gaaaaagtcc tggcggacgt
      661 tctcgcgcgc tggcaccgga tgaaggggga ggaagtctat ttcctgaccg gggtggacca
      721 gcatgggcag aaagtgcagc agacggctga aaagctgggc attacgccgc aggagcatgt
      781 caacaatatc acggggcaat tcctggcgct gtgggaccgc ctgggaatca gcaatgacgg
      841 atgggcctcc acgacggatc cgcgccacaa ggcctgcgtg cagaaaatcc tgacggacct
      901 gaaggataag ggccagcttt acaaaaaatc ctacaaggga ttctactccg tgcgccagga
      961 acagttcctg acggataagg aacgggacgc ggaaggcaac ttcggcccgg aatgggggga
     1021 agtggtggaa ctggaggaag aaaactggta tttccgcctg accgaccatg tggaatggat
     1081 gaagcagttc gtggaaaaga gcagtgattt tgtccttccg tccttccgca aggcggaagt
     1141 gctcaacgcc attgactttg actcggacct ctgcatttcc cgccccaaga gccgcctttc
     1201 ctggggaatc gaattcccgt tcgaccccga attcgtcacc tacgtctggt ttgacgccct
     1261 gatcaactac atttccttcg ccggctacct ggcggagccg gacagcggcc tgccggattt
     1321 tgccaagctc tggcccgcgg actgcgaagt catcggcaaa gacatcctgg tgcccgccca
     1381 cggcgtgtac tggcccgcca tgctgcatgc catgggcttt tccgacgagg aaatgcccac
     1441 cctgctcgtg cacggctggt ggaacatcaa cggggaaaaa atgtccaagt ccatcggcaa
     1501 cgtggtggac cccaacctgc tggcggaaca gttcggcccg gaacccgtgc gctattacct
     1561 ggtgcgggac atcaccaccg ggaaggacgc caactttgat gcagaccgcc tggtcatgct
     1621 gtacaatacg gagctggcca acgacctcgg caacctgtgc aaccgctcca tcaacatgac
     1681 gcgccgctat tgcgactccg ttatctccag cgcggaggca tatgatgacg aagcctccaa
     1741 agccctgcgc gccaccatgg accaggccgt caccctgttt tccaaataca tggacgacaa
     1801 catggcctcc gatgccctgg cggccctgaa cgcccaggtt tccgcctgta acgcgtacat
     1861 tgagcagcag cagccctggc agctggccaa ggatgaagcc tccgccccgc gcctccgctg
     1921 cgtgctgcgc catttgctgg aatgctgcgc ccagacgggc tatctcatcg gctgcgtcct
     1981 ccctgacgcc tccgcacgca ttctgggcca gctcaacgcc ggggacctgt tccggagcct
     2041 tacgccggcc gcgctggagt ggggaatcct ccccgcaggg caccgcatca atgaccccgc
     2101 ccccgttttt ccccgcattc tttcggaaga ggaaaaggcc aaactggctg aaaaagcggc
     2161 cagggcggca aaaaaacaag gctgattccc gtgaattcct ccgcatcctt cgtcaaatcc
     2221 ctgctggccc aatgctgcct gggcggcatt tgcgagtggg tggtctgccc cggcgcgcgc
     2281 aacatggcgc tgcttcaggt gctggctgcc gcggaggatc tggtgaaatg gactcacttt
     2341 gacgaacggt ccgccgcctt ctttgcgctg ggccgcattc aggatatggg gctgcccgtg
     2401 gccgtggtca ccacctccgg aacggcggcg gcggaactgc tccccgccgt ggtggaggcc
     2461 tactaccagc gccgtcccct gctcctgctc acggcagacc gtccggcggc ctgccggggt
     2521 tccgccgctc cccaggccat cgaacaagtt gacctgttcg gcatttacgc acccacgatt
     2581 gacctggaaa cgccggaaag cctgccggaa gacattctgc aagactggga ttacgcctcc
     2641 cccctccata tcaacgtgtg cctgccggac ccggaccctg cctggaaccc cggcagctgc
     2701 gacctttacc cggcggaacc tccggaggaa aacggattcc ggggttccct ggcggagctg
     2761 gcccgggccc tgcgcttcaa atcgcgcgac gggctggtag tcatgatcgg cggcctggac
     2821 cccacggaac aagccccggc ccggtggctg gccaacgaac tgaaagcccc cgtggtggcg
     2881 gacgccacct ccggactccg ggaagagctg gcccatctgg ctctgacgga cgcagacgcc
     2941 ctgctgaggg aacacccgcc cgccgtcctg ctcagattag gggacgtgcc cgtgggccgc
     3001 ttctggcgcg acctggagga cattccagcc acggaagtct tctccgtcac ccgcaccggc
     3061 ttctccgggc tggcccgccc ctcctccgtc gtaaccggag acctggaagc cattctgcat
     3121 gcgctggggg atatagatac cgtgggcgac gtcaacggcc tgcgcgccat gaacaaacgc
     3181 aggaaagccc tgatggagga actgctcatc acctgcccgg aaagcgaaca ggccatggtg
     3241 cgctccttct cctgctttgc cgcggacgga gactgcattt acctgggcaa ctccatgccc
     3301 gtgcggtact ggaacagctt cgcccagaca tccatcccca cggaaaacgt ccgcgccaac
     3361 cggggagcca acggcattga cgggcaaatc tccggttttc tgggggtctc cgcccggtgt
     3421 tcccgctcct gggctctcgt gggcgacctg accgccatgt atgattccaa tgccctggca
     3481 ctccttcccc aactggacag ggggacccgc gtcctcggcg tcatcaacaa cggcggaggg
     3541 ggcatcttcc gcaccctccc gggagcagac ggccagccgg aaaccatgag gaagctgctt
     3601 gtacaacccc acgcccattc cttcaaggcc attgcggaac aatggggaat gcgctacc
//
LOCUS       DAOMSU010000214         3652 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2516_length_3652_cov_2.986934, whole genome shotgun sequence.
ACCESSION   DAOMSU010000214 DAOMSU010000000
VERSION     DAOMSU010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2516_length_3652_cov_2.986934"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="PK077_08480"
     CDS             complement(<1..321)
                     /locus_tag="PK077_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24373.1"
                     /translation="MDILVSTLCDFAADYQGKLCIQGGFDSLVARQFPVVHPVCAVAL
                     RICLTPEDEGTHELGLSIVDADGTPLDKERMPIKINFPVPAFPEGASFFTRNLIMNFQ
                     GLRFE"
     gene            514..>3652
                     /locus_tag="PK077_08485"
     CDS             514..>3652
                     /locus_tag="PK077_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN24374.1"
                     /translation="MIQWFFDIPSKLIHWRLNLVRTLGAYVPWLRRDDGSMKVHAGTL
                     VDLFATILSGRRHLSARDADAALDILRYTFPEVEHRWLSNRFERSMRASLTVADVLAS
                     AASGRDETERMAIALEVLSLLINTGDARMTGDLFDQVTYGLELPGTANHLRQLLMTPD
                     VEAQEPAYSVGFSSGIGAEVSLPEQDKGISFRLIRCSRLVLVVNDGTRPIVVRGRHLQ
                     PGGVMPLTNGQVVLLPSGPLSFEDFAFFLDCKRSGKQDVCYISLDGGSLQISRVRSRA
                     SEVRVNMGLACEVEVLRPEVEFAVDGKRLYPGESVRMAYYSSFTLDGEGPFSMGEVQN
                     SMSDIGRRFRLDPGNRKLRVTNLPEKARKGDMLLTPGLAPGAVLEVSFSSATNSGWLE
                     VVEASMPLLLNGRPVRGRVSLRDGDVVHLNAYHALRCRFSAGVLDEEYHAIRTLSVEG
                     VTKEFLRSGRVLDNIDLAVKRGEMVCILGPSGSGKSTLLSMLAGQLPPTRGCIRYNNQ
                     LLYSAPDLIRPYIAFIPREDILDAAMSVSEHISQATMIRRPRLNRSERVRRVNAILKF
                     LGLTHIASRRVGEMNARTISDGERTRLNLGLDLAGIADVFLLDEPISGLSTSDAKLVM
                     QTMQNMSRDKMVIATMHRPSTAILNQFHKVLVLDHGGQMAYWGDVPGMMRYFRKAAAD
                     MAIDVSEEARTAGGADYVFEVLEAPLSWHDRRRRQHPRLWQERFEGYRFRNVMGHHHE
                     GGAPRTLYEGTLEFPPAPRRSVIQLWRLFRIWAVRTFLGRVRSRMGLYTMLLEGPVLA
                     LLISMTLRASSSPEYTFATALHIPSYLFLATIVAMFFGLTGAASEVLKDRALLKRESN
                     AKVFVTGYVLAKALVLTGLSAVQSALFLWVGNSILEIHEMFLIYLGTMTLTAFVGVSL
                     SLLVSVFARTERAALNMVPLLLIPQILMAGAIVRFDEMNQFIPWSAHRTDEHGRLKPG
                     RVPLVAEFCPLRYSFETMVVDQASKNVWERERETIQEKVDDLKERPHLDDREFEEFKL
                     LKMALLHIAAIGAEGP"
BASE COUNT          685 a         1028 c         1163 g          776 t
ORIGIN      
        1 ttcaaaacgc aggccctgga agttcatgat caggttgcgg gtgaagaaag aggctccttc
       61 cgggaaagcg ggaaccggga aattgatctt gatgggcatg cgttccttgt ccagcggcgt
      121 gccgtcggca tcaacgatgc tcaggcccag ttcgtgcgtg ccttcgtctt caggagtgag
      181 gcagatgcgc agggcaacgg cgcagacggg atgcacgact gggaactggc gggcaaccag
      241 ggagtcaaaa ccgccctgga tgcagagctt gccctggtag tcggcggcga agtcacaaag
      301 agtagaaact aaaatatcca tgttgtgata atgttgatgt atttggctag ctttcacgcg
      361 gaaagcatga acattggaca gaactatact tcctccagac ctattgcaag ataaagctgt
      421 agaaaatatt gtcatttctc cgtgaaattc cggttgcgct tgagatgggg cgggaatccc
      481 tgtactactg gttccggcaa cgcgtttcct ggcatgatcc aatggttttt tgacattcct
      541 tccaagctga ttcactggag gctgaatctg gtcaggacgc tgggcgccta cgtgccgtgg
      601 ctgcgccgtg acgacggctc catgaaggtt catgccggaa cgctggtgga cttgttcgcc
      661 actattttaa gcggacgccg ccatttgagc gcccgtgatg cggacgccgc tctggatatt
      721 ctgcgctata ctttcccgga agtggagcac cgctggcttt ccaaccgttt tgagcggtcc
      781 atgagggcca gcctgacggt tgcggatgtc ttggcctccg cagcttccgg cagggatgag
      841 acggaacgca tggcgatagc tctggaagtg ctctccctgc tgattaatac gggagatgcc
      901 cgcatgacag gggatctgtt tgaccaggtg acgtacggtc tggaactgcc gggcacggca
      961 aaccacctga ggcagctgct gatgacaccg gacgtggagg cccaggaacc ggcgtacagc
     1021 gtcggttttt cctccggcat cggtgcggag gtatccctcc cggagcagga caagggcatt
     1081 tccttccgcc tgatccggtg ttcccgtttg gtgctggtgg tgaatgacgg cacccgcccc
     1141 attgtggtgc gcgggcgtca tttgcagccc ggcggggtga tgccattgac caacgggcag
     1201 gtggtgcttc tgccgtccgg tccgctgagt tttgaagact tcgcgttttt tctggattgc
     1261 aagcgttcag gcaagcagga tgtatgctat atttccctgg acggaggctc cctgcaaatc
     1321 agccgggtgc gttcccgcgc cagcgaggtg cgcgtgaaca tggggctggc gtgcgaggtg
     1381 gaggtgctga ggccggaagt ggaattcgcg gttgacggga aacggctgta ccccggtgaa
     1441 agcgtgcgca tggcctatta ctcctccttc acgctggacg gggaagggcc tttctccatg
     1501 ggggaggtgc agaattccat gtcggacatc gggcgccgtt tccgcctgga tcccggaaac
     1561 cgcaagctgc gggtgaccaa cctgccggaa aaggcgcgca agggagacat gctgctgact
     1621 cccgggctgg cccccggagc ggtgctggag gtttcttttt ccagcgccac caattccggc
     1681 tggctggaag tggtggaggc ctccatgcct ctgctgctca atggccggcc tgttcgggga
     1741 agggtttccc tgagggatgg ggacgtggtg cacctgaatg cctaccacgc gctgcgctgc
     1801 cgtttttccg ccggggtgct ggatgaagaa taccatgcga tcagaaccct cagcgtggaa
     1861 ggggtgacca aggaattcct gcgctccgga cgcgtgctgg acaatattga cctggccgtg
     1921 aaacgcgggg aaatggtgtg catcctgggg cccagcggat ccgggaaaag cacgctgctc
     1981 tccatgctgg ccggccagct gccgcccacc cggggctgca tccgttacaa caaccagctt
     2041 ctgtacagcg ccccggatct tatccggccc tacatcgcct tcattccccg ggaagatatt
     2101 ctggatgcgg ccatgagcgt ttccgagcac atctctcagg ccaccatgat acgccgtccg
     2161 cggctgaacc ggtcggaacg cgtcaggcgc gtgaacgcta ttttgaaatt cctgggcctg
     2221 acgcacatcg cctcccgccg cgtgggtgag atgaacgccc gcaccatttc cgacggggag
     2281 cgcactcgcc tgaacctggg gcttgacctg gcaggcattg cggatgtttt cctgctggac
     2341 gaacctatca gcggcctgtc cacgtcagat gccaagctgg tcatgcagac catgcagaac
     2401 atgagccgcg acaaaatggt aatcgccacc atgcaccggc ccagcacggc tattctgaac
     2461 cagttccaca aagtgctggt gctggaccac ggcggccaga tggcttactg gggggacgtg
     2521 ccgggcatga tgcggtattt ccggaaggcg gctgcggaca tggccattga cgtctccgag
     2581 gaggcgcgga cggccggagg cgcggactac gtatttgagg tgctggaagc ccccctctcc
     2641 tggcacgacc gccgcaggcg gcagcacccc cgcttgtggc aggagcgctt tgaaggctac
     2701 cggttccgca acgtgatggg gcatcatcat gaagggggcg cccccagaac gctgtatgaa
     2761 ggcacgctgg aatttccccc cgcccccagg cgcagcgtca tacagctctg gcggcttttc
     2821 cgcatctggg ccgtgcgcac cttcctgggg cgcgtgcgga gccgtatggg gctgtacacc
     2881 atgctgctgg aagggccggt gctggccctg ctgatttcca tgaccctgcg cgcctcttcc
     2941 agcccggaat atacgtttgc cacggccctc cacatccctt cctacctgtt cctggcgacc
     3001 attgtggcca tgttcttcgg cctgaccggg gcggccagcg aagtgctcaa ggaccgtgcc
     3061 ctgctcaaga gggagagcaa tgccaaggta tttgtgaccg gttatgtgct ggcaaaggcg
     3121 ctggtgttga cggggctttc cgccgtgcag tccgccctgt ttctttgggt ggggaactcc
     3181 attctggaaa tccatgaaat gttcctgatt tacctgggga cgatgaccct gacggccttt
     3241 gtcggcgtga gcctgtccct gctggtttcc gtcttcgcca ggacggagag ggcggcgctt
     3301 aacatggtgc ctctgcttct gattccccag atattgatgg ctggagccat tgtccgcttt
     3361 gatgaaatga accagttcat cccatggtcc gcccaccgta cggatgaaca cgggcgtctt
     3421 aagccggggc gtgtgccgct ggtggcggaa ttctgccctt tgcgctactc ctttgaaacg
     3481 atggtggtgg atcaggcgtc caaaaatgta tgggagaggg agcgtgaaac catccaggag
     3541 aaagtggacg acctgaagga acgtcctcat ctggacgaca gggaatttga agaattcaag
     3601 ctgctcaaga tggcgctgct ccacattgcc gctattggag cggaggggcc gg
//
LOCUS       DAOMSU010000215         3645 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2521_length_3645_cov_2.415042, whole genome shotgun sequence.
ACCESSION   DAOMSU010000215 DAOMSU010000000
VERSION     DAOMSU010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2521_length_3645_cov_2.415042"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="PK077_08490"
     CDS             complement(<1..1145)
                     /locus_tag="PK077_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRN24375.1"
                     /translation="MAAQSRNIAELENASVWRLLIQYSLPSIAGMVVYSLYNIIDSVF
                     IGHGVGPLALSGLAVTFPIMNLTFALGTLVGIGGAAISSIRLGRKDQEGTERTLGNVL
                     IMSLIAAVVLVIPTLLFLTEILTAFGASGQTLTYAYDFMLITLLGLPVTYVFFNLNHV
                     MRATGYPKKAMGSTLLSVGINIILAPIFIFWFKWGITGAAVATLLAQVVGMVRVLLHF
                     SDAASTVHFRRGIWQLRKTIVTGILSIGMAPCLVNVCGSAVTVAINRQLADHGGDLAI
                     GAYGIINRILILFAMLVIGLTQGMQPIIGYNHGAMKPARVLLTLKYGVLAGTSFTTLG
                     FLACVFFPRGIAGLFTDDASLISLAANGLLICVLAFPLIGSQIIIGN"
     gene            1323..1769
                     /locus_tag="PK077_08495"
     CDS             1323..1769
                     /locus_tag="PK077_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24376.1"
                     /translation="MTISVFPFLRLVSVCGAALMGSSCLPMFGAGQVLAGSSLADGLL
                     PSSSLDLVWRGDIRSFPSSGLRTPRGFKVSPADSYSLLMEARPGIAGEWACYHDRSNY
                     YWAFVAGKKEEEAGLYAVRRGVAVNGISGEIEDAPAAKDVRPPSVM"
     gene            1881..>3645
                     /locus_tag="PK077_08500"
     CDS             1881..>3645
                     /locus_tag="PK077_08500"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRN24377.1"
                     /translation="MKFLLLSFAWMCMACAGAWGQDTAPSFPANGANYRLFPAERPPL
                     VPKPQQLRWDGRAIPVQSARILAPSPSKTSYPEQMKFIVSELKSFLADHRVKVAPDGT
                     FAVKFVKGEVKTGTENSKLKEEAYSLRVTSGGALITAMDTRGFYYGMKTLEQLLLRRG
                     GTTTIAACDIVDWPDFEIRGFMNDVGRNYMPLPLIARELDSMAQLKLNVYHFHFTENP
                     GWRLESKIYPELNDSRNYTRMPGKFYTQKEFKQLVEYCRLRNILLIPEMDMPGHSQMF
                     RKALNVKMSDEKATKALVALIKELCSLVPKEKMPIIHIGTDEVRGRDEQVNNEILKEY
                     IHAVESCGRIPMRWQPGLTPKGYNGSIQQLWSGRQNRGAWPTDGAKYVDSLETYLNHL
                     DPFETAMTMYFRRACPFRNAEGLGMMLCSFPDLEITDPRNQVLQTPVYAGMAFVSEPL
                     WNNPHEKVQGDPNQDEYMKYFSNLPVQGDPLLKGFADYENRVLAIRDRFFMDKEFNYV
                     RQANIPWKLLGPIPNGGKTETEFAPEEDNKAGKMKDSYEIDGVIYEWSGDDYTGATII
                     FKHYCDFPTLFNGAKMGAYPHK"
BASE COUNT          788 a          949 c         1121 g          787 t
ORIGIN      
        1 aagttcccga tgatgatctg gctgccgatg agcggaaagg ccagcacgca gatgagcagg
       61 ccgttggccg ccaggctgat gagcgaggcg tcatccgtga agaggccggc aatgccgcgg
      121 ggaaagaaga cgcaggccag gaagcccagc gtggtaaagg atgttccggc cagaactccg
      181 tatttaagtg tgagcagaac gcgggccggc ttcatggctc cgtggttgta gccgatgatg
      241 ggctgcattc cctgagtgag gccgatgacg agcatggcga acagaataag aatgcggttg
      301 atgatgccgt aggccccgat ggccaggtcc cccccgtgat ccgcaagctg cctgttgatg
      361 gcgacggtga ccgcgctgcc gcagacgttt accaggcagg gagccatccc gatggacagg
      421 atgccggtga cgatggtctt gcgcagctgc cagatgccgc ggcggaagtg gaccgtgcta
      481 gccgcatcgg aaaagtgtaa cagcacgcgc accatgccga cgacctgggc gagaagggtg
      541 gccacggctg ctccggtaat gccccatttg aaccagaaga tgaagatggg agccaggatg
      601 atgttgattc ctacggaaag aagggtggag cccatggctt ttttagggta gccggtagct
      661 ctcatcacat gattgaggtt gaagaagacg tatgtgacgg gcaggcccag caaggtaata
      721 agcatgaagt cgtacgcata ggtgagggtt tgcccgctgg cgccgaaggc ggtcaggatt
      781 tccgtaagga aaagaagcgt gggaatcacc agcaccacag cggcaatcag actcatgatg
      841 aggacgttgc caagcgtacg ctcagttcct tcctggtctt ttcttcccag ccggatggag
      901 ctgatggccg ccccgccgat gcctaccagc gtgcccaggg cgaaagtcag gttcatgatg
      961 gggaaggtga cggccaggcc ggacagggcc agaggcccca cgccgtgacc gatgaacacg
     1021 ctgtcaatga tgttgtacag ggagtatacc accatgccgg cgatggaggg cagggagtac
     1081 tggatcagca gacgccatac ggatgcgttt tccaattctg caatgttgcg tgactgggct
     1141 gccatgggcg ttcttccttt caaaacgggc agcaaactat aaaaacgcgc ggatgggttg
     1201 tcaagctgtc cacctgtccg gaaaacggcc tttttccccc agttccagag agagtgctgt
     1261 ttttgattga ctgtggaagc ttttgggagt tgagtccttt ccgccttttt cagaagcacg
     1321 ccatgaccat atccgttttc cctttcctgc gccttgtctc cgtttgcggc gccgctctta
     1381 tgggttcttc ctgtctgccc atgtttggcg ccgggcaggt gctggcggga agcagcctcg
     1441 cggacggtct gctgccttct tcctctctgg atctggtgtg gaggggagac atccgttctt
     1501 ttccctcttc cggtttgagg acgccccgcg ggttcaaagt ttctcccgcg gattcttatt
     1561 ccctgctgat ggaagcgcgc ccggggattg ccggagagtg ggcctgctat catgaccgca
     1621 gcaattacta ctgggctttt gtagccggaa agaaggagga ggaagcgggg ctttacgccg
     1681 tgcgccgcgg cgtggccgta aacggtattt ccggagaaat tgaagatgcc ccagctgcga
     1741 aggatgtccg gccaccctcc gtcatgtaag cgcaggttct ggtctttttg caaaaaaatg
     1801 ctgccggagc cccgtttccc ggcaggttgg acacgatagg tttgacaggc agccgttatt
     1861 gcgggcgtat tattggccgc atgaagttcc ttttattatc gtttgcatgg atgtgcatgg
     1921 cttgcgccgg agcatggggg caggatacgg ccccgtcctt tccggccaac ggagccaatt
     1981 acaggctgtt tccggcggag cggcctccgc tggttcccaa accccagcag cttcgctggg
     2041 acggcagggc tattcccgtg cagtccgcgc gcattctggc tccgtctccg tccaagactt
     2101 cctatccgga gcagatgaag ttcatcgttt ccgagttgaa gtcttttctg gcggatcacc
     2161 gcgtgaaggt ggctccggac gggacgtttg ccgttaaatt cgtcaagggg gaggtgaaaa
     2221 ccggcacgga aaattccaag ctgaaggagg aagcctattc ccttcgtgta acttccggcg
     2281 gcgcactcat tacggcgatg gataccagag gattctacta cggcatgaaa acgctggagc
     2341 agcttctttt gcgacgcggc gggacgacga ctattgccgc ctgcgatatc gtggactggc
     2401 cggattttga aatccgcggg ttcatgaacg atgtgggacg caattacatg ccgctgcctc
     2461 tgattgcacg ggagctggat tccatggcgc agcttaagct gaacgtttac cacttccatt
     2521 ttacggagaa tcccggctgg cggctggaat ccaaaattta tccggagctg aacgattcca
     2581 ggaattatac gcgcatgcct ggcaagtttt acacgcagaa ggagtttaag cagctggtgg
     2641 aatactgccg cctgcgcaac atcctgctga ttccggagat ggatatgccg gggcacagcc
     2701 agatgttccg caaggcgctc aacgtgaaga tgagcgatga aaaggccacc aaggccctgg
     2761 tggccctgat caaggagttg tgttccctgg ttcccaagga gaaaatgccc attatccaca
     2821 tcggcacgga tgaggtgcgc ggcagggatg agcaggtgaa caatgagatt cttaaggagt
     2881 acatccatgc agtggagtcc tgcggccgca ttcccatgag atggcagccc ggcctgacgc
     2941 cgaagggcta taacggttcc atccagcagt tatggtccgg ccgtcagaac cgcggcgcgt
     3001 ggcctactga cggagcgaag tacgttgatt ccctggaaac ttacctgaac catcttgatc
     3061 cgtttgaaac ggccatgacg atgtatttcc gccgggcatg tccgttccgg aatgcggaag
     3121 gactgggcat gatgctgtgc tctttcccgg acctggaaat cacggatccg cgcaaccagg
     3181 tgcttcagac gcccgtttac gccgggatgg cgttcgtttc cgaacccttg tggaacaatc
     3241 cccatgagaa ggttcaggga gatcccaacc aggacgaata tatgaagtat ttttccaatt
     3301 tgcccgtgca gggagatcct ctgctgaaag gatttgcgga ttacgagaac cgagtgctcg
     3361 ccatccggga ccgctttttc atggataagg agtttaatta cgtgcggcag gcgaatattc
     3421 cctggaaatt gctggggcct attcccaacg gcggtaagac ggaaacggaa ttcgccccgg
     3481 aagaggacaa caaggcaggg aagatgaagg attcctacga gattgacggc gtcatctatg
     3541 agtggtccgg agacgattac acgggagcca ccatcatttt caagcattac tgcgatttcc
     3601 cgacgctgtt caatggagcg aagatgggag cttatcccca caaga
//
LOCUS       DAOMSU010000216         3622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2539_length_3622_cov_2.369779, whole genome shotgun sequence.
ACCESSION   DAOMSU010000216 DAOMSU010000000
VERSION     DAOMSU010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2539_length_3622_cov_2.369779"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2529
                     /locus_tag="PK077_08505"
     CDS             <1..2529
                     /locus_tag="PK077_08505"
                     /inference="COORDINATES: protein motif:HMM:NF019193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylglucosaminidase domain-containing
                     protein"
                     /protein_id="HRN24378.1"
                     /translation="VILPVGEITDWPDIEFRGTVEGFYGTPWSHEARLSQLRFYGQNK
                     MNTYIYGPKDDPYHSSPHWRDPYPADQAAQIRELVKVAKENHVDFVWAIHPGKDIKWT
                     EEDMNNVIKKFEMMYKLGVRSFAVFFDDIFGEGKRGDMQALLLNKINDEFVKVKKDVT
                     PLVMCPTEYNRGWADPKPGTYLDILGDRLDPSIHVMWTGNSVCHDITLEGQQWVNRRI
                     KRPSYVWWNFPVTDYCRSNLCMGRVYGLASEPGAKESMGGFVSNPMDKPEASKVSLFG
                     LADYSWNINGFKSDEAWKEGVRRLFPKAAEAMQVFVNHNSDQGPNGHGYRREESVEIE
                     PVVKRVLEAAREGKIEKKDTALLKKEFARMAAAAPVIRARANNPRLMKEIGAWVNAFE
                     QLGHAGQHAVAALEENNAREAATRLVQATQALAAMDGISRRHNQEGQLYRSAVKTGSR
                     VMAPAVNELADIVSKKVFPAIAGAPALSPKPLVKGGSMDKAELFCDGDRGSFWHSGAY
                     GQPGDWYGVDYGMPIPVRSVEVLMGRNDKDGDYVEKGQLEGSRDLETWKPLGPETSGM
                     QVVWKAPKPVLLRAVRYRVIEPKKTGNGRSVWTAVREIAVNTPPSAMASSNVSGLEGV
                     SVQKSDKIVRINRVMETHKMKPGEFISLQLDGPTDATWLEVNLERDDVNSWAEVVLDV
                     EGSSKPVVQKLDKQGKNFIARGNQLPKGIKGMKLVNKSGREQDIVLNMFKFDVPPADP
                     GTSLVSLSDRNLKTVYRADKPLDVVVPNLDNPKATKVIVVGSAAFAIQARRGEGAWTP
                     VGKRNAGPGVSEFAIPAGTSAVRLTYKAPQPEAIINEVIFSSRK"
     gene            2777..>3270
                     /locus_tag="PK077_08510"
     CDS             2777..>3270
                     /locus_tag="PK077_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRN24379.1"
                     /translation="MKAFPAELPLLVKQLLLQRGFTGGMETELFLDPRLSHLSDPFLM
                     GEMRAAVDRIFRAVDEGETVCIYGDYDVDGVTSVALLRAILMAYDLDPQYFIPVRSRE
                     GYGLSEAGIKRCLCECADPPSLLITVDCGTSSVKEVDMLNSLGIDVIILDHHEAGPLG
                     RPDAA"
     assembly_gap    3271..3280
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3281..>3622
                     /locus_tag="PK077_08515"
     CDS             <3281..>3622
                     /locus_tag="PK077_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRN24380.1"
                     /translation="EKGVDFIICDHHMPDDELPRAVAVVNAKIEENSPYTYLCSAGVV
                     FKLAHALLKERKLKTFDLKLYLDLVAVATVADIVPLVAENRILVRHGLGRLAHSRHTG
                     LKTLTEIAGIRP"
BASE COUNT          784 a         1023 c         1092 g          713 t
ORIGIN      
        1 gtgatcttgc ccgttgggga aattacggat tggccggaca ttgaattccg cggaacggtg
       61 gaaggttttt acggcactcc ctggagccat gaagcccgtc tgagccagct gcgcttttac
      121 gggcagaaca aaatgaacac atacatctac gggccgaagg acgatcccta ccactcttcc
      181 ccccactgga gggaccccta tcccgcggac caggccgccc agatcaggga actggtgaag
      241 gtggcaaagg aaaaccacgt ggattttgtg tgggccatcc atcctggcaa agatatcaag
      301 tggacggaag aagacatgaa caacgtcatc aaaaagtttg agatgatgta caagctgggc
      361 gtccgttcct ttgccgtgtt ttttgacgat atcttcggcg aaggcaagcg cggggacatg
      421 caggcccttc tgctgaacaa gatcaatgac gaatttgtca aggtgaagaa ggacgtcacc
      481 cccctggtca tgtgccccac ggagtacaac cgcggctggg ccgatcccaa gccgggaacc
      541 tatctggaca ttctggggga ccgtctggac ccctccatcc acgtcatgtg gacggggaac
      601 tccgtttgcc atgacattac gctggaaggc cagcaatggg tgaacaggag aatcaagcgc
      661 ccttcctacg tctggtggaa cttccccgtc acggactact gccgttccaa cctctgcatg
      721 ggccgcgtgt atggccttgc ctcggaaccg ggagcgaagg aatccatggg cggtttcgtc
      781 tccaacccca tggataagcc ggaggcctcc aaggtctccc tcttcggcct tgcggactac
      841 tcctggaaca tcaacgggtt caagtctgat gaggcctgga aggaaggagt caggcgcctg
      901 ttcccgaaag cggcggaagc catgcaggtt tttgtgaacc acaactccga ccagggccct
      961 aacggtcacg gctaccggcg tgaggaatcc gtggaaatag agcccgtagt caagcgcgtg
     1021 ctggaagccg cccgggaagg caagattgag aagaaggata cggccctgct caaaaaggaa
     1081 tttgcccgta tggccgccgc tgcgcccgtc atccgtgcca gggcgaacaa tccgcggctg
     1141 atgaaggaaa taggcgcgtg ggtgaatgcc tttgagcaac tggggcatgc cggtcagcac
     1201 gctgtagcgg cgctggagga aaacaacgcc agagaagccg cgacccggct ggtgcaggct
     1261 acgcaggctc tggcggccat ggatggaatt tcccgccgcc acaaccagga aggacagttg
     1321 tatcgttctg cggtaaagac cggttcccgt gtgatggccc ccgccgtcaa tgaactggcg
     1381 gatattgtct ccaaaaaagt ttttccggcc attgccggcg cgccggccct ttctcccaag
     1441 cccctggtca aggggggcag catggataag gcggaactct tctgtgacgg ggatcgcggc
     1501 tccttctggc actccggcgc ctacggccaa ccgggcgact ggtatggcgt ggactacgga
     1561 atgcccatcc ccgtgcggag cgtggaagtg ctgatgggcc gcaatgacaa ggacggcgac
     1621 tatgtagaaa aagggcagct ggaaggctcc cgcgacctcg aaacctggaa accgctgggg
     1681 ccggaaactt ccggcatgca ggtggtctgg aaggctccca agccggtttt gctccgtgcc
     1741 gtacgctacc gcgtcattga accgaaaaaa acaggcaacg gccggtctgt ctggactgcc
     1801 gtgcgggaaa tagccgtcaa cacgcctcct tccgctatgg cttcctccaa tgtgtcgggg
     1861 ctggagggcg tttccgtgca gaaatccgac aaaatcgtgc ggatcaaccg cgtgatggaa
     1921 acgcataaga tgaaacccgg ggaatttatt tccctgcagc tggatggccc cacggacgct
     1981 acctggctgg aagtcaacct ggaacgggat gacgtcaact cctgggcgga agtggtgctg
     2041 gacgtggaag gttcttccaa gcccgtagtc cagaagctgg acaaacaggg caaaaacttc
     2101 attgccaggg gcaaccagct tcccaaggga atcaagggga tgaagctggt taataaaagc
     2161 ggcagggagc aggatattgt tctgaacatg ttcaaatttg acgttcctcc cgcggacccc
     2221 ggcaccagcc tggtttccct gagcgacaga aacctgaaaa cggtttaccg tgccgacaag
     2281 ccgctggacg tagtcgttcc caatctggac aatcccaagg ccaccaaagt aatcgtggtg
     2341 ggatctgccg ccttcgccat ccaggcccgc cgcggagaag gcgcctggac gccggtcggc
     2401 aaacggaacg ccggccccgg tgtctctgaa tttgccatcc ctgccggaac ttctgccgtg
     2461 cgcctgacct ataaggctcc ccagccggag gcgatcatta atgaagtcat tttttcctcc
     2521 cggaaataac cgtctcaggc gctggcgcct tacgccgaac tccgtccgga acgaaccggg
     2581 cggagttttt tttgaaaggg ccgggcgcgc ggcgtgcgga ctgtttccgg aaacggaagc
     2641 ggacgcttct tttctccgct ttggccgtct gttgcggaaa caaccttgac cctgttggat
     2701 gcggggctaa aatactgcca tgacgccggg ttttcattgg actctacgcc cttccgtgaa
     2761 ggaggatgat ccggtcttga aagcgtttcc tgcggaactg cccctgctgg tcaagcagct
     2821 cctgctgcag cgcggcttca ccgggggaat ggaaacggaa ctctttctgg accccaggct
     2881 ttcccacctg agcgaccctt tcctgatggg ggaaatgagg gctgccgtgg accgcatttt
     2941 ccgggccgtg gatgaagggg aaaccgtgtg tatttatggg gactatgatg tggacggtgt
     3001 cacctccgtg gcgcttctcc gggccatttt gatggcttat gacctggatc cccagtattt
     3061 tattcccgtc cgttcccggg aaggctacgg actcagcgag gccggcatca agcgctgcct
     3121 ttgcgaatgt gcggaccctc ccagtctgct tattacggtg gactgcggca cttcctccgt
     3181 gaaggaggtg gacatgctta acagcctggg gatagacgtc atcattctgg accaccatga
     3241 ggcgggccct ttggggcgtc cggatgcagc nnnnnnnnnn gagaagggcg tggatttcat
     3301 catctgcgac caccacatgc cggacgacga actgcccaga gccgtggcgg tggtcaatgc
     3361 caaaattgag gaaaacagcc catatactta cctgtgcagc gcgggcgtag tcttcaagct
     3421 ggcgcatgcc ctcctgaagg agaggaaact gaaaaccttt gacctgaaac tttatctgga
     3481 cctggtggcc gtggcaaccg tggcggacat tgttcccctg gtggcggaaa acaggattct
     3541 ggttcgccac ggcctgggaa ggctggctca cagccgccat acgggcctca aaaccctgac
     3601 ggaaatagcg ggcatccgcc ct
//
LOCUS       DAOMSU010000217         3613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2544_length_3613_cov_2.627319, whole genome shotgun sequence.
ACCESSION   DAOMSU010000217 DAOMSU010000000
VERSION     DAOMSU010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2544_length_3613_cov_2.627319"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="PK077_08520"
     CDS             <1..458
                     /locus_tag="PK077_08520"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN24381.1"
                     /translation="TGLLIVQHSGGIQTIIGKSDNTISTGNYASGTWTFSNGPILNTG
                     TTYYAMFTETDLSTISIGDCWDNNFFSINGSRRIDNSMDNAAGTGPSVNQLALVGSNW
                     QKDFIARNDNMVPTGLQIHTSSVPEPASALLLAFGMALLPFRRRKSLTL"
     gene            477..1787
                     /locus_tag="PK077_08525"
     CDS             477..1787
                     /locus_tag="PK077_08525"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRN24382.1"
                     /translation="MNLHLSSLAALLLASYLPAQATVPDHSPSTAFIRSGLPVVDLDQ
                     WTEVQVVIDKEKGKYLGHLTTLLLKDGKTILCVYPKGHGSGEIILKKSTDGGKTWSER
                     LPVPESWKTSREVPTLYETEDSRGKRRILLFSGIQGGNRNTAHRNRMAVSEDNGTTWS
                     ELTPIPKQAGGIVVMSDLIPLKTGKGHYMASYHANARGKDEHGEFHTIEQYVTFTEDG
                     GLTWTSPRVIFPGTRDMHLCEGGFVRSPDGKTIALLLRENSRHHNSQIMFSGDEGKTW
                     TPPKELPAALCGDRHQILPLPDGRLLVQFRDVPPTRKKGQAASPTEGDWVGWIGRWED
                     LKNGTEGSYKIRFKDNRNGWDCAYPAAELLPDHTLVCTTYGHFDKGELPYILSVRFKI
                     SDTDSMVRQYAGNKHPKIKNDTGAGETVFDPNDPDSVNRLLKRS"
     gene            1888..2997
                     /locus_tag="PK077_08530"
     CDS             1888..2997
                     /locus_tag="PK077_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRN24383.1"
                     /translation="MNNTCPGETAQSRTVAKLKHRLRSGQFTNAMPGERELSKALAAG
                     RGTIRKALAQLQEEGFIAAPDKNRARKIIRTSTSPVQPLPLLEKRVCFLSSVPIRDIP
                     QSIWVELYQLEKALRIEGMALQLVEATWAEKNNPDMRLEKLETEYPGCCWILYRATEE
                     IQLWFKRKGLPCLVRGVSYPSSNLPYLDTHWEVLAGHAAGYLYQKGHRRIALCIPDSK
                     LRGNRLMEQGFLSFQGEHYTPYVIRMPSDAIKAAQELNRIDLADTGITALIPTRSRML
                     ISIISWAATMAKSIPEEISLLSLVHEPYLNHITPPITSYRSPAEKTTRRLIRMIEALL
                     EHRRISNSLILPELCPGQSISTLNSPPPAFESIKQ"
     gene            complement(3104..>3613)
                     /locus_tag="PK077_08535"
     CDS             complement(3104..>3613)
                     /locus_tag="PK077_08535"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRN24384.1"
                     /translation="MKGMVNETSIAETDNGGVVMVARHWGAGNCRRIAWSRDGGETWG
                     QVEDAPELFCDSTQNSLMTYSLSDQPAYGGKSRILFSGPSAGRRIKGQVAMSYDNGKT
                     WPVKKLLGEVGFAYSSLAMVEPGIVGVLYEENQEHIKKLKFVPITIEWLTDGKDAGLA
                     PGKKAPVLK"
BASE COUNT          936 a         1084 c          876 g          717 t
ORIGIN      
        1 gaacaggcct gctcatcgtg caacattcag gcggtatcca aaccattatc ggcaaatcgg
       61 acaataccat ttctactgga aactatgctt ccggaacatg gacttttagc aacgggccaa
      121 ttttaaatac aggcactact tattacgcca tgtttactga aacggatctg tccaccatca
      181 gcataggaga ctgctgggac aacaatttct tctccatcaa cggttcccgg cgcatcgaca
      241 attccatgga taacgcagcc ggaacggggc cgtctgtcaa ccagctcgct ttagtcggct
      301 ccaattggca aaaggacttt attgcccgta acgataacat ggttcccaca ggtcttcaaa
      361 ttcatacatc gtccgtaccg gaaccggcaa gcgcattgct gcttgcattt ggcatggcac
      421 ttctgccctt cagacgccgc aaatctctca ccctttaatc acaacatcca tttcccatga
      481 atctgcacct atcatccctg gcagcgttgc tcctggcgtc ataccttccg gcgcaggcca
      541 ccgtaccgga ccattcccct tccacagcgt tcatccggag cggcctccct gtcgttgatc
      601 tggatcaatg gacagaagtc caggtagtca ttgacaagga aaaaggaaaa tacctgggac
      661 atctaacgac gctattgctg aaagacggaa aaaccattct gtgcgtttat cctaaagggc
      721 acggctccgg ggaaatcatc ctgaaaaaat ccacggacgg aggcaaaaca tggagcgaaa
      781 ggctgccggt tccagaatca tggaaaacca gcagggaagt acccacacta tatgaaacgg
      841 aagattcccg gggaaagcgc cgcattctgc ttttcagcgg cattcaggga ggaaacagaa
      901 acacagccca cagaaaccgg atggcggtca gtgaagacaa cgggacaaca tggtccgaac
      961 tgactcccat tcccaaacag gccggaggca ttgttgtcat gagcgacctg attcctctaa
     1021 aaacgggaaa agggcattat atggcctcct accatgccaa tgcccggggc aaagacgaac
     1081 atggagagtt tcacaccatt gaacaatatg tcacctttac ggaagacggt gggctgacct
     1141 ggacctcccc ccgggtcatt ttcccgggga caagggacat gcacctgtgt gagggaggtt
     1201 ttgtccgcag cccggacgga aaaacaattg cgctgctgtt acgggaaaac agcaggcacc
     1261 ataactccca gatcatgttt tccggagatg aaggcaaaac atggactccc cccaaagaac
     1321 tgccggcagc cctgtgcgga gaccgccacc agattcttcc cctgcctgac ggaagactgc
     1381 tggttcaatt cagggatgtt cccccgacca ggaagaaagg gcaggccgcc agcccgacgg
     1441 aaggggactg ggtgggatgg ataggccggt gggaagacct gaaaaacggc acggaaggct
     1501 catataaaat ccgttttaag gacaaccgca acggatggga ctgcgcctac ccggctgccg
     1561 aactattgcc ggaccatacc ctggtatgca ctacctacgg acactttgac aaaggggaat
     1621 tgccatacat cctctccgtc agattcaaaa tcagcgacac ggacagcatg gtcaggcaat
     1681 acgcggggaa caagcacccc aaaatcaaaa atgacacagg agcgggagaa accgtttttg
     1741 accccaatga tccggactcc gttaatcgcc ttctgaaacg ttcctgaaca tcatattcct
     1801 ccatgctcct ggaaaacgcg gaacgggaaa aacacaaccg gcagaaccgc ttgccaccaa
     1861 acacgaccag aaagtatgat tccccccatg aataatacat gccccgggga aacggcccag
     1921 tcaagaaccg ttgccaaatt aaagcaccgc ctccggtccg ggcaattcac aaacgccatg
     1981 cccggagaac gggaactaag caaagcgctc gccgccggca ggggaaccat acggaaagcc
     2041 ctcgcgcagc tgcaggaaga gggcttcata gcggctccgg ataaaaacag ggccaggaaa
     2101 atcatcagga cttctacaag ccctgtgcag cctctgcccc tgctggaaaa acgcgtttgc
     2161 ttcctctcct ccgttcccat ccgggacatc ccccaaagca tttgggtgga actctaccaa
     2221 ctggaaaaag cgctgcggat cgaaggtatg gcgcttcagc tggttgaagc cacatgggca
     2281 gaaaaaaaca atccggacat gcggctggaa aaactggaaa cagagtatcc aggctgctgc
     2341 tggatactct accgcgccac ggaagaaata cagctgtggt ttaagcggaa aggcctcccc
     2401 tgcctggtcc ggggagtttc ctacccttcc tccaatctgc cgtatctgga tacgcattgg
     2461 gaagtgctgg ccggtcacgc ggccggctac ctgtaccaga aaggacaccg tcggatcgcg
     2521 ctctgcatac cggattccaa gctgagaggc aacaggctca tggaacaggg attcctctcc
     2581 ttccaggggg aacattatac cccctatgtc atccgcatgc cgtcggatgc cattaaggca
     2641 gcccaggaac tcaaccgaat tgacctggcg gatacgggta ttaccgcctt aattcctacc
     2701 cggagcagaa tgctgatatc catcatctcc tgggcggcca ccatggctaa aagcatcccg
     2761 gaggaaataa gtctgctcag cctggttcat gaaccttacc tgaaccatat cactcctccc
     2821 attacctcct accgctcccc tgcagaaaaa acgacgcgca gacttatccg gatgattgaa
     2881 gccctgctgg aacatcgccg tatctccaat agcctcatcc tcccggagtt atgcccaggc
     2941 caatccatca gtacgttgaa ttccccccct cctgcctttg aatccatcaa gcaataaggc
     3001 ctgatctgct ttcttccata acgctccgct acggaagaaa gcattcaatc tccccctccc
     3061 ggagacccct ccggaaggga aaaacgtatt gcccggcttt cctctatttg agaacagggg
     3121 cttttttgcc gggagccagc cccgcatcct tcccgtccgt cagccattca atggtaatgg
     3181 gaacaaactt cagcttttta atatgctcct ggttctcctc ataaagcacc ccgacgatgc
     3241 cgggttccac catggcaagg ctggaatagg caaaaccgac ctcgcccagc aatttcttca
     3301 ccggccaggt tttgccgttg tcatagctca tggccacctg tcccttaatg cgccggcccg
     3361 cgctgggccc ggaaaacagg atgcggcttt taccgccgta ggcaggctgg tcgctcaggg
     3421 aatacgtcat cagggaattc tgggtactgt cgcaaaacag ctccggagcg tcctccacct
     3481 gtccccaggt ctccccgcca tcccgggacc acgcaatacg gcggcaattg cctgcgcccc
     3541 aatggcgcgc aaccatcacc acgccgccgt tatccgtttc cgcaatggac gtctcgttca
     3601 ccatgccttt cat
//
LOCUS       DAOMSU010000218         3609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2550_length_3609_cov_2.969893, whole genome shotgun sequence.
ACCESSION   DAOMSU010000218 DAOMSU010000000
VERSION     DAOMSU010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2550_length_3609_cov_2.969893"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            351..2513
                     /locus_tag="PK077_08540"
     CDS             351..2513
                     /locus_tag="PK077_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRN24385.1"
                     /translation="MAIFGSSNLSDSGDLLIFNKVSYKTLLMFEKELGRDRMTYLVEE
                     GLPPDTATASHLESTKADGILFQAGGSDPVALRSAIMERINEGRRVVFLPGPVSHVKG
                     SISQIPPRVIKALEALHISPVPVYAGFYTNSVLDAEADTDAQADIQIHILPKLAPGAE
                     MAARLTSAWLECSAQAYATLPQLHGSLSALLFRSLKLHSDCRVIDGIDDTTLTYGQLL
                     AISVAFAKRLKKITSNRRVGIILPPGKGAAIANLGCLFAGKTPVNFNYSASEGAFASS
                     VKQSGVDWFITADTFMRKLQNFPWPPQRDLILMEREIPLLKGSAKRWGLAIKFLTAGF
                     MIKKLGLDAPTGADEAVLMFTSGSSGEPKGVPLTHHNLLSNISQCSSRITLEPQSRFL
                     GSLPVFHCFGITIGLWYPMIGGYDMVTYPSPLEAKRLGTLIKQYGISLVVTTPTFLRG
                     FMKRCEPDTFKTVRYLIVGAEKLPEDLSAAFREKFGIIPCEGYGLTEASPVCSVNFID
                     PAPSNAAGDFIPGMKKSSVGALLPGIAIRITSPHTGRVVPITTSGMIWLKGPNIFPGY
                     LGGPETDRDIFVDGWLKTGDIGSADEFGFLKIEGRISRFSKIGGEMVPHEALEAAIMN
                     IWNLDPADEERRIAVVTIPDPVKGEAVALLTTLVTDYVHQARTLIRHGLIDQGLPALW
                     CPKEIIPVERIPVLPSGKLDIKQCRMLAYEALNIPFEP"
     gene            2517..>3609
                     /gene="xseA"
                     /locus_tag="PK077_08545"
     CDS             2517..>3609
                     /gene="xseA"
                     /locus_tag="PK077_08545"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRN24386.1"
                     /translation="MEFPEETPAAPKPITVKQLVYRLRDTVSIAVGTQWVVGELSNVK
                     HHTSGHVYFTLKEQGAEIFCAFFKAAASKCPIRLQEGMKVHVLGSATVYPDRGQLQLV
                     IRQVKAAGQGDLQTRFLELKAKLQREGLFDAEHKKKIPTFPRAIGIVTSPTGAVIQDI
                     RHVLERRAPWVKAYLLPVRVQGAGAEHEIAAAVRAWSGAPFNGLPPVDVLIVGRGGGS
                     IEDLWNFNEETVARAIYECTVPVISAVGHDTDFTIADFAADLRAPTPTAAAELATPDG
                     PEWLRKLSRMEQALHASARHSLLRSKLKLDVYLRGKLLDAYSLLSPYSQRLDDMEETL
                     QNAATTRIFHNALHINKLEHQLQMRHPAHR"
BASE COUNT          806 a         1109 c          932 g          762 t
ORIGIN      
        1 ccagcagaga gacgttcgtt tcccctctcc ggtctcccag gtcctgcgcc gcgtttacgc
       61 ggatttccgc cagcgtttca tgtccgaaga tgttgctgcc taccaggccc attcatcagt
      121 aagtaaatat cggaacagct ggtttacgga aacgccttcg caggagataa cgccaaatcc
      181 aggcaatccc gtgaaaaaca atactcttcc cataaaaaac gtaccgagcc gggaaaggct
      241 tccgagaccg gtccggataa aggaaaagca accgggaatg cacctttcct ccttgctccc
      301 ggctcttcaa gttgctaaaa tgcttcaagc aaatttttta tccttttccc atggccattt
      361 ttggttcctc caatttatcc gactcaggtg atctgttgat tttcaacaaa gtatcttata
      421 aaaccctgct gatgttcgaa aaagaactgg gcagggaccg catgacctac ctggtggaag
      481 aaggtctccc cccggatacg gccaccgcct cccatctgga atccactaaa gcggacggca
      541 tcctgttcca ggccggaggc agcgaccctg tcgcgctgcg ttccgccatc atggaacgca
      601 ttaacgaagg cagaagagta gtatttctgc ccggtcccgt ctcccatgtg aaaggttcca
      661 tcagccaaat tcctccgcgg gtcatcaagg ccctggaagc cctgcatatt tcccccgtac
      721 cggtctatgc cggtttttac acgaactccg tgctggatgc ggaggccgat acggacgccc
      781 aggcggacat ccagatacat attctcccca aactggcccc cggggccgag atggcagcac
      841 gcctgacctc cgcgtggctg gaatgttccg cccaggcata cgccacgctg cctcagctcc
      901 acggttccct gtccgccctc cttttccgca gcctcaaact tcattccgat tgccgggtca
      961 tcgacggcat tgacgacact acgctgactt acggccagct gctggccatt tccgtagcct
     1021 ttgccaagag gttgaaaaaa attacctcga atcgccgggt cggcatcatt cttccgccgg
     1081 gcaagggagc ggccatagcc aatctgggct gcctgttcgc cgggaaaaca ccggtgaatt
     1141 tcaattattc cgcctcggaa ggagcctttg ccagctctgt aaagcaatcc ggcgtggact
     1201 ggtttattac tgcggatacc ttcatgcgaa agctccagaa tttcccgtgg cccccccagc
     1261 gggatctgat cctcatggag cgggaaattc ccctgcttaa aggttccgcc aaacgctggg
     1321 gcctcgccat caaattcctg acagcggggt tcatgattaa aaaactgggg ctggacgcac
     1381 ctacaggcgc ggacgaagcc gtgctgatgt tcacttccgg ctcttccggg gaacccaagg
     1441 gcgtgccgct gacccaccac aaccttcttt ccaacatctc tcaatgttcc tcccgcatta
     1501 cgctggaacc gcaaagcagg tttctgggaa gcctgcccgt attccactgc ttcggcatca
     1561 ccatcgggct atggtatccg atgatcggcg ggtacgacat ggtcacctat ccatcccctc
     1621 ttgaggccaa acggctggga acccttatca agcagtacgg aatcagtctg gtagtcacca
     1681 cgcccacctt cctgcgcggt ttcatgaaac gctgcgaacc ggacaccttt aaaaccgtcc
     1741 gctacctgat cgttggcgcg gaaaaactgc cggaagacct ttccgccgct ttccgggaaa
     1801 aattcggcat cattccatgc gaaggctacg gcctgacgga agcctctccc gtctgttccg
     1861 tcaacttcat tgacccggcg ccatccaatg ccgccggaga cttcattccc ggcatgaaaa
     1921 agagttccgt aggagccctc cttcccggaa tcgccatacg catcaccagc cctcacacgg
     1981 gacgcgtggt tcccatcact acttccggca tgatctggct gaaaggaccg aacatctttc
     2041 ccggctatct gggcggtccg gagacggatc gcgatatttt cgtggacggc tggctaaaaa
     2101 ccggagacat aggttccgca gacgaattcg gcttcctgaa gattgaagga cgcatttccc
     2161 gcttttccaa aataggcggg gaaatggtgc ctcatgaagc cctggaagca gccattatga
     2221 acatttggaa tctggatccg gcggacgaag aacggcggat agccgtcgtc accattccgg
     2281 accccgtaaa aggggaggcc gtagccctgc tcaccaccct ggtgacggat tacgtgcacc
     2341 aggcgcgcac cctcatcagg cacggcctga ttgaccaggg gctgccggcc ctctggtgcc
     2401 ccaaggagat tattcccgta gaacgcatcc cggtgctccc atccggcaag ctggatatca
     2461 agcaatgcag gatgctggcg tatgaagcgc tgaacatccc ctttgaaccg taatttatgg
     2521 aattcccgga ggaaacgccc gccgcgccca aacccatcac agtcaaacag ctcgtttacc
     2581 gcctgagaga cacggtcagc atcgctgtgg gcacccagtg ggtggtggga gaactgagca
     2641 acgtcaagca tcacaccagc ggccacgttt atttcaccct gaaggaacag ggagcggaaa
     2701 ttttctgcgc ctttttcaag gcagcggctt ctaaatgccc catacggctc caggaaggga
     2761 tgaaagtcca tgtcctgggc agcgccaccg tatacccgga ccgggggcaa ctccagctgg
     2821 tcatcaggca ggtaaaagct gcaggacagg gagatttgca gactcgcttt ttggagttga
     2881 aagccaaact ccagcgcgag gggttgtttg atgcggaaca caaaaagaaa attcccacat
     2941 tcccccgcgc catcggcatc gtcacctccc ccaccggcgc cgtcatccag gacatacggc
     3001 acgtattgga acgccgtgcc ccgtgggtga aggcttatct gctgcccgta cgcgtgcagg
     3061 gggcaggagc ggagcacgaa atagccgcag ccgtgcgcgc ttggtccgga gcgcccttca
     3121 acggtctccc gcccgtggac gtgctcattg taggccgcgg cggcggctcc attgaggatt
     3181 tgtggaattt caatgaggaa acggtggcgc gcgccattta cgaatgtacc gttcccgtga
     3241 tttccgcagt gggtcatgat acggacttca ccattgccga ttttgcggcg gatctgcgcg
     3301 cacccactcc caccgcagcc gcggaactgg ccacaccgga cggccctgaa tggctccgca
     3361 agctgtccag aatggaacag gctcttcatg catccgctcg gcattccctc ttgcgttcca
     3421 agctgaaact ggatgtttac ctgcgaggga aactgttgga tgcgtattcc ctgctctctc
     3481 cttattctca gcgcctggac gatatggaag aaactctgca gaatgccgca accacgagaa
     3541 tttttcataa tgcgcttcat attaacaaat tggaacatca actgcaaatg cgccatcccg
     3601 cccacagga
//
LOCUS       DAOMSU010000219         3596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2564_length_3596_cov_2.778029, whole genome shotgun sequence.
ACCESSION   DAOMSU010000219 DAOMSU010000000
VERSION     DAOMSU010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2564_length_3596_cov_2.778029"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="PK077_08550"
     CDS             complement(<1..1380)
                     /locus_tag="PK077_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRN24387.1"
                     /translation="MPAVFDGFVERWNRAVERELRRELAEGERKEKLLVVSRGEGDGR
                     RLAEQARSVERIRKRLLLKDHIRLLPLEDVPDGLEWQTGMEEPDVGDPRAVKGGRVRL
                     WINTPFPGTLRAFGPGSENFFNYSAIDNVWLPLVGLHPETFRPIPGLADRWALSADGK
                     TVFYHLDPEAAYSDGRIVKAQDFLLNICLRTSGFARDPFWTTLFRSMYDQITVYGDSV
                     IALTLPSRGPLLPYMACADFHPAHPGFYHDFNALFLERYQWKVPPNTGGYMVVPAKIR
                     IGESITLARVKNWWAKGRKYYRHSCNADQVEHVFVNMENKAVEMFRRGELDIMNVRKP
                     EVWETGLELPEVHRGYIDKYSLEANYACPPYGLYLNCSDKLLKNPDIRRGLAHSVNMG
                     LVIDTLFRGNMRRLGSYMEGYGDLTLPLKAPEYSKKKAMEYFARAGYREMGTDGVLKN
                     ERGERLVVEL"
     gene            complement(1653..>3596)
                     /locus_tag="PK077_08555"
     CDS             complement(1653..>3596)
                     /locus_tag="PK077_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HRN24388.1"
                     /translation="GQSSLAESAKKEDSKPSVKTETMQVMPELTMASHFVGVPYNRSG
                     VSVSIINPEEFQKAGIETLTGALSQTPGVFTLDGGGTWQRGSVSNTVIRGMNKDTYTL
                     TMVDGMRISDANMSGNKLLGITNLFTVGNVEVVKGAQGAVFGSGAIGGVVAMDTPEGE
                     GDPVTRIFAEAGSFGSFNSYITSSGKIKKLSYFVGVGFETTENDPSIYPSIYDNRTGM
                     NDFRQWQEAVRLGYDINDKVKVSFTYRRLDSYFEYPTPYVDYNQWPSVPDPHLYNTED
                     KNRSNLVTGRVDAEISKLWSTSFMVGHYNMDYSCHTPGFDFQPNVMRNRRFQAEWRNA
                     LTWNKEWKTIAGMAWDRSDYMSENNYVAKDEWQSTLAFFAEQMWSPTDSFDASVALRL
                     EHDSVWNNHFTWRYSNSWKVTGKDSPTRIFGSVGSGFRAPTYFEQYAANYGYVGNPDL
                     DVSKSLGGDLGVEQRLADGHYASVTGFWTRIDDEIGTMSVGTWPSSYTTYTNYSHCTS
                     YGVEVAFKGQFKDAWNSGYYANYTFTMPKRDSIGKYETIQMANTARHTINAEVYTSPV
                     EKLTVGFGVTAAMGRTDYNYARLDNFFTARLFARYQATDNVSLHVRLENLFDQKFIMT
                     NDYNFGPREARGFGIFGGVTVEF"
BASE COUNT          846 a         1032 c          933 g          785 t
ORIGIN      
        1 cagttccacc accagccgtt ctccccgttc atttttcagc acgccgtcag tccccatttc
       61 ccggtagcca gcgcgggcaa aatattccat ggcttttttc ttgctgtatt ccggagcttt
      121 cagcggaaga gtcaggtctc cgtacccctc catgtaggaa ccgagccttc tcatattccc
      181 gcggaacaaa gtatcaatca ccaggcccat gttcacggaa tgcgccagcc cgcggcggat
      241 atccggattt ttcaacagtt tgtccgagca attcagatac agtccgtacg ggggacaggc
      301 gtaattggct tccagactgt atttatctat atagccccgg tgcacttccg gcagttccag
      361 acccgtttcc cacacctccg gcttacggac gttcatgatg tccagttccc cgcggcggaa
      421 catttcaacc gccttgtttt ccatgttcac aaacacatgt tccacctggt ccgcattgca
      481 ggaatggcgg taatacttcc tgcccttcgc ccaccaattt ttcacgcggg ccagggtaat
      541 gctttctcct atccggattt tagcgggaac aaccatgtag ccacccgtat ttggagggac
      601 tttccactgg tagcgttcca gaaacagagc gttgaaatca tggtaaaaac cgggatgggc
      661 cggatgaaaa tccgcacagg ccatgtaagg cagcagcggc ccgcgggacg gcagcgtcag
      721 ggcaatgacg gaatccccgt ataccgtaat ctgatcatac atggaacgga acagggttgt
      781 ccagaacgga tcccgggcaa aaccggacgt acggaggcaa atgttcagca gaaaatcctg
      841 cgctttcacg atgcgtccgt ccgaataggc cgcttccgga tccagatgat aaaaaacggt
      901 ttttccgtct gcggacaagg cccagcggtc cgccaggccc ggaatgggac ggaaggtttc
      961 cggatgaagc cccacgaggg gaagccacac gttgtcaatg gcggagtaat tgaaaaaatt
     1021 ttcacttccc ggcccgaaag cacgcaaagt tccgggaaac ggagtattga tccacaaccg
     1081 gactcttcca cccttgacgg cgcgcggatc acccacatcc ggctcttcca ttccggtttg
     1141 ccattccaat ccgtccggaa cgtcttccag aggaagaagg cggatatggt ctttcaataa
     1201 cagccttttc ctgatgcgtt ccacggaacg agcctgttcc gccaaacgcc ggccgtcccc
     1261 ttccccgcgg gaaacgacca gaagtttttc cttcctctcc ccttcagcca attcacggcg
     1321 cagctcccgt tccacggccc tgttccagcg ttccacaaaa ccgtcaaata cggcaggcat
     1381 ccccctgccg tccatccggc cggaaaattc atgccaaaca ttcttctcac gctgggaaaa
     1441 gtaaaagaca cctcccagaa agacggaaag ggccaatgcc cccagtatgg atatccagcg
     1501 caacacaact cagatggccg ccacatgtta accgattccg gaaactacgg aaaacagaaa
     1561 acccatcttc cgcgcggaac cgttcacttc gcctgaaaca gtccgcaaga cggaagatgg
     1621 gggagcaatc aacaattaat attgaaagga acttagaatt cgaccgtcac accgccgaag
     1681 attccgaatc cacgggcttc acgcgggccg aagttataat cattcgtcat gatgaacttc
     1741 tggtcaaaca ggttttccag acgcacatgg agggacacat tgtccgttgc ctggtaacgg
     1801 gcaaacaggc gcgccgtaaa gaaattgtcc agacgggcat aattgtaatc cgtgcgtccc
     1861 atggcggccg ttacgccgaa gccgaccgtc agcttttcaa ccggggaggt ataaacctct
     1921 gcgttgatgg tgtggcgggc ggtattggcc atctgaatgg tttcgtattt gccgatggaa
     1981 tcgcgcttgg gcatcgtgaa ggtgtaattg gcataatagc cgctgttcca cgcgtccttg
     2041 aactgtccct tgaacgccac ttccacgccg taggaagtgc agtgggaata attggtgtaa
     2101 gtggtatagg aactaggcca ggtgcctacg ctcatggtgc cgatttcatc gtcgatacgg
     2161 gtccagaagc ccgtcacgga agcataatgg ccgtccgcca gacgctgttc cacccccaga
     2221 tcgccgccca gggacttgga cacatccaga tcagggttgc caacataacc ataattggcc
     2281 gcatactgct caaaataggt gggcgcgcgg aaaccggaac ccacggaacc gaaaatacgg
     2341 gtgggggaat ccttgcccgt cactttccag gaattggaat aacgccacgt aaaatgattg
     2401 ttccagacgg aatcatgttc caggcgcagg gccacgctgg cgtcaaagct gtccgtgggt
     2461 gaccacattt gctccgcaaa gaaggcaagc gtgctctgcc attcatcctt ggcaacgtaa
     2521 ttgttttcgc tcatgtagtc ggaacggtcc caggccatgc cggcgatggt tttccattcc
     2581 ttgttccacg tcagcgcatt acgccactcc gcctgaaaac ggcggttgcg catcacattg
     2641 ggctggaaat caaatccggg agtatggcag gaatagtcca tgttgtaatg ccccaccatg
     2701 aaactggtgg accacagctt ggaaatttcc gcatccacac gtcctgtcac cagattgctg
     2761 cggttcttgt cttccgtatt gtacagatgg ggatccggaa cggaaggcca ctggttataa
     2821 tccacataag gcgtgggata ttcaaagtag gaatccaggc ggcgataggt aaagctcacc
     2881 ttcaccttgt cattgatgtc atagcccagg cgcacggctt cctgccactg gcggaaatcg
     2941 ttcatgcctg tcctgttgtc ataaatggac ggatagattg acggatcgtt ttccgtagtt
     3001 tcaaacccaa cgcctacaaa ataggaaagc ttcttgatct tgccggagga agtgatgtag
     3061 ctgttgaaag agccgaagga accggcctcc gcaaaaatcc gggttacggg atcgccttcc
     3121 ccctccggag tatccatcgc gacgacgcct ccaatggcgc cggaaccgaa gacggcgccc
     3181 tgcgcgcctt tcaccacttc cacattgccc accgtaaaga gattagtgat gcccagcagc
     3241 ttgttgccgg acatgttggc atcactgatg cgcatgccgt caaccatagt cagggtgtag
     3301 gtatccttgt tcatcccgcg gatgacggtg ttgctcacgg aaccgcgctg ccaggtgccg
     3361 cctccgtcca gcgtgaaaac gccgggcgtc tgggaaaggg ctcccgtcag ggtttcaatg
     3421 cccgccttct ggaattcttc cggattgatg atggaaacgg acactccgga gcgattgtac
     3481 ggcacgccca caaagtgaga cgccatggtc agttccggca tcacctgcat ggtttccgtt
     3541 ttgacggagg gcttggagtc ttccttttta gcggactcgg caagagagga ctggcc
//
LOCUS       DAOMSU010000220         3592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2570_length_3592_cov_2.479502, whole genome shotgun sequence.
ACCESSION   DAOMSU010000220 DAOMSU010000000
VERSION     DAOMSU010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2570_length_3592_cov_2.479502"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..2244
                     /gene="yidC"
                     /locus_tag="PK077_08560"
     CDS             103..2244
                     /gene="yidC"
                     /locus_tag="PK077_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HRN24389.1"
                     /translation="MMKWLLITLVKGYQTFISAPLHALGGPGSGCRYTPTCSQYFIQA
                     VRIHGAWRGFLLGSRAPGAPPTLINYPSFLFLSPTPIKLYHIMDRTAWIIIGICAALL
                     GLNIYMGNNKKAETAPIPAPQAAAAANAPVTGVHPGAGTSAPEQASVNKALEEDKTAP
                     ITLVATQEMNGRQEPFITYTFNRIGGSIGTVTLHNDIVDSQKVANHNITINEAQQRGI
                     GELVFNMDATQDPSYDNTVYKEVKRTADSVTLEGYDPARQLFISKTYTLHPVKNLEGE
                     VLPGSKYLIRLTVSLLNKSPNVQDLRYMGIFGGSAYPIAKSEPKDTYTHFFYHADGSL
                     EQEVPSYFTGGFFSSAKARVLEGPLQDLTYAGVMSQYYATILLPQVQSRGSTVYATRQ
                     EFPLTHEGNTLVPGVTVAMGIPNLTMAPNEIKTLTYDIYTGPKLNAYLRDLNLSYPAI
                     SDIMAYGWLVFLSVPMNWLLNLFHGWFGNWGVAIICMTIVVRALIWPLHKKSYMAMKR
                     MSLVQPEMVKLKEKYPDDPQKVNMEMMKLYQKYGINPASGCVPMLIQIPIFFAFYRVL
                     QYSAELRGQPFCLWMTDLSLPDTVGHLFGIPINILPLIMAVTMIIQMRMTPQAGERSQ
                     RIIMNLMPVMFFLFCYNFASALALYWTTQNLISIGQTALIRRLPMPVLSPAKKKKAGF
                     FQKMMEQQRAALEEQQRKAKGRNMRNITPKK"
     assembly_gap    2358..2367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3007..>3592)
                     /locus_tag="PK077_08565"
     CDS             complement(3007..>3592)
                     /locus_tag="PK077_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HRN24390.1"
                     /translation="AFLAPGVTFELVGEANDFVGKGLSGGKIIVLPPDHISYVPSDNV
                     IAGNVIGYGATSGSIFLHGQAGERFAIRNSGAKMVVEGIGDHGCEYMTGGRVAVLGKV
                     GVNFAAGMTGGLAYVYDADNNFDQKCNLGSVDLETVMPHSADEAELLQLIYEHYMATR
                     SRRAYDLLNNWPEERGKFIKVFPVEYRHALAMHS"
BASE COUNT          873 a         1134 c          821 g          754 t
ORIGIN      
        1 ctggagggcc gtggaagcca gctacgccga cctggaacgg gactggcgga aagccgcccg
       61 aaaactcaaa ttgcaacttc tttcccggga acaagacaag acatgatgaa atggctgctc
      121 atcacccttg tgaaggggta ccaaaccttc atcagcgccc ccctgcatgc cctgggggga
      181 cccggaagcg gctgccgcta tacacccacc tgttcccagt acttcatcca ggccgtccgc
      241 atccacggcg cctggcgcgg ctttcttctt ggctcccgtg cccccggcgc gccccccacg
      301 ctgatcaatt acccttcttt ccttttcctt tcaccaactc caatcaagct atatcacatt
      361 atggatcgca cagcatggat catcataggc atctgcgccg cccttctggg cctgaatatt
      421 tacatgggca acaataaaaa ggctgaaacc gcccccattc ccgcaccgca ggctgccgcc
      481 gcagccaatg ccccggttac cggcgtccac cccggagccg ggacctccgc cccggaacag
      541 gcctccgtca acaaagccct cgaagaagac aaaaccgccc cgatcactct ggtagccacg
      601 caggaaatga acggcaggca ggaacctttc atcacctaca cgtttaaccg catagggggc
      661 tccatcggca cggttaccct gcacaacgac attgtggact cccaaaaagt cgcgaaccat
      721 aacatcacga tcaacgaagc ccagcagcgg ggcatcggag aactcgtctt caatatggac
      781 gccacccaag atccttccta tgacaatacc gtgtataagg aagtgaaacg cacggcggac
      841 tccgtaaccc tggaaggcta tgaccccgca cggcagcttt tcatctccaa aacatacacc
      901 ctgcaccccg tcaaaaatct ggaaggggaa gtactgcccg gcagcaaata cctcattcgc
      961 ctgaccgttt ccctgctcaa taaatctccc aatgtccagg atctgcgcta catgggcatc
     1021 tttggcggga gcgcctaccc catcgccaaa agcgaaccca aagataccta cacccatttc
     1081 ttttaccatg cggacggctc cctggaacag gaagtgcctt cctatttcac gggcggattt
     1141 ttcagcagcg ccaaagcccg cgttctggaa ggccctcttc aggatctgac ttatgccggc
     1201 gtcatgagcc aatattacgc caccatcctt cttccccagg tccagtccag gggatccacg
     1261 gtgtacgcca cccgccagga attccctctg acgcatgaag gcaacaccct ggtgcccggc
     1321 gtcaccgtgg ccatgggaat ccccaacctg accatggccc ccaatgaaat caagacactc
     1381 acctacgata tttacaccgg ccccaaattg aacgcttatc tgcgtgacct gaatctctcc
     1441 tatcctgcca tcagcgacat catggcatat ggctggcttg tgttcctgag cgttccgatg
     1501 aactggctgc tcaacctctt ccacgggtgg ttcggtaact ggggcgtggc catcatctgc
     1561 atgaccatcg ttgtccgcgc gctcatctgg ccgctgcaca aaaaatccta catggccatg
     1621 aaacgcatgt ctctggttca gccggagatg gtcaaactca aggagaaata tccggacgat
     1681 cctcagaagg tgaacatgga aatgatgaag ctgtaccaga aatacggcat caacccggcc
     1741 agcggctgcg tccccatgct tatccagatt cccatcttct tcgccttcta ccgagtgctc
     1801 cagtattccg cagaactgag gggacagccc ttctgcctgt ggatgacgga tctctccctg
     1861 cctgataccg tgggccacct gttcggcatt cccatcaata ttctgcctct tatcatggcc
     1921 gtaaccatga ttatccagat gaggatgact cctcaggcgg gggaacgctc ccagcgcatt
     1981 attatgaacc tgatgcctgt gatgttcttc ctgttctgtt acaatttcgc ttccgccctg
     2041 gccctgtact ggaccacgca gaatttgatc tccatagggc aaacggccct tatccgccgc
     2101 ttgcccatgc ccgtgctctc tcccgccaaa aagaaaaagg ccggtttttt ccaaaagatg
     2161 atggagcagc agcgtgcggc cctggaagaa cagcagcgca aagctaaagg ccgcaacatg
     2221 cgcaatataa cgcctaaaaa gtaggaatcc ctgctctgcc ttccgcctct gcaagtgcgt
     2281 ccgcccctcc gcggccgcac tttttcatga aaaggcgcag gaaaaaacat tctgccggcg
     2341 caagcctcaa aaaagggnnn nnnnnnngta gctgcataac aagcaaatcc cattatccac
     2401 gcctcaataa gaaaaaggaa ttcccttacg gaaatcctgc aaaaaaccgg ccctctctat
     2461 caggagaggg ccggttttcc tcgtcatata ttcattgtgt agttaattgg agcagcctgg
     2521 cagaaaaaac caaacacccg ttggagcaaa cctaacggat tatgaattcg cggcaattcc
     2581 ctgaataact aaataacaag aataccaatc agttaccttt caattcactt ttttatcaat
     2641 tgccatccag acgaattccc aaaaccaaaa ctccactaac acatttccat tcaaactttc
     2701 ctgttgactt cgaattcata atccgctcag aaccgggcat tgctggaaca tttgaccttc
     2761 ctcctgacat caactgcaac caccatcccc ctcgcagccg catttctcta tcaactgaca
     2821 gtatggtcat gaaaacctcc ttatccggaa cattcgtctt caaatgcgga atggcttaag
     2881 caggaaaagc ggcgccaaca tgcccggcca tgaaacggca tacaggccta tcaaaaaatg
     2941 ggggcctccg cacccgcgga ggcccctgcc ttaccccaaa caacagaaaa ctgctgcatg
     3001 cttctttcag gaatgcatgg ccagggcatg ccggtattcc accgggaata ccttgatgaa
     3061 tttgccgcgt tcttccggcc agttgtttaa caggtcgtaa gccctgcggc tcctggtagc
     3121 catgtaatgc tcgtaaatga gctgaagcag ttccgcttca tcagcggaat gcggcatgac
     3181 cgtttccagg tccacgctgc ccaggttgca tttctgatcg aagttgttgt ccgcatcata
     3241 cacgtaggcc aggccgccgg tcatccccgc ggcgaaattg acgcccactt tgccgagcac
     3301 cgccacacgg cccccggtca tgtattcaca gccgtggtcg ccgatgcctt ccaccaccat
     3361 tttggcgccg ctgttgcgga tggcgaaacg ttccccggcc tgcccgtgaa ggaagatgct
     3421 gccggaagtg gccccatagc cgatgacgtt tccggcgatg acgttgtctg acggtacgta
     3481 ggagatatga tccgggggga gcacgatgat tttgccgcca gacaagccct tcccgacaaa
     3541 gtcattcgcc tccccgacaa gttcaaaggt gacgccaggc gccagaaaag cc
//
LOCUS       DAOMSU010000221         3581 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2583_length_3581_cov_2.131594, whole genome shotgun sequence.
ACCESSION   DAOMSU010000221 DAOMSU010000000
VERSION     DAOMSU010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2583_length_3581_cov_2.131594"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..1079
                     /locus_tag="PK077_08570"
     CDS             72..1079
                     /locus_tag="PK077_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN24391.1"
                     /translation="MPEPLLQVDNLKMYFPVRSGIFLRQAGWVKAVDDVSFNIYPGET
                     LGLVGESGCGKSTIGKSIVRLLKPTGGSIRFNGNNIARLSQRKMRPLRPHIQMVFQDP
                     AESLNQRQSIGQIVAEPFVIHRMGTPAARREWVRGLLDRVGLPDSAIDRFPFEFSGGQ
                     RQRIGIARALTLNPSLIILDEPVSALDVSVQSQVLNLLLELQEERKLSYLFIAHDLAV
                     VKHISDRVAVMYLGKIVEMSHAETIYQSPKHAYTKALLDAIPEPDPSRANKHQPLPGD
                     VPSPINPPLGSAFGHRIQHPSYPETVGADLTPVEIEPGHWVAPDPCALEPEDWNRIRQ
                     R"
     gene            complement(1198..>1969)
                     /gene="alr"
                     /locus_tag="PK077_08575"
     CDS             complement(1198..>1969)
                     /gene="alr"
                     /locus_tag="PK077_08575"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRN24392.1"
                     /translation="EHFNSLGALYGKTVHVHLGVDTGMGRSGFLPSELHGLTDKLKQF
                     SYLDLEGVFSHLSAASDDISFTHGQISGFTEAVNELSQGHRYKFRHLCSSAAVFNYKV
                     PCANMARLGRILYGYSPMPSPYNKELRPTMTLYSRITLIRTLPGNHGVSYNHCYITKG
                     STKVATIGIGYADGYLQYLSNQGARVYINGQYCPVLGRVTMDQIMVDVTYMDHVETGD
                     LVEIMGPNVSWEELTRRANTIPSNVLTSISARVPRIYV"
     assembly_gap    1970..1979
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1980..2311)
                     /locus_tag="PK077_08580"
     CDS             complement(<1980..2311)
                     /locus_tag="PK077_08580"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRN24393.1"
                     /translation="MSCTSPPRAWAQIDTNALRHNLNVVRRVMPDHRLMAIVKAEAYG
                     HGLEGVVKALDGEDCAFFGVATVAEACRVRDAGAKTCPFILGPCFEGEREEIVQNGWR
                     AALSSMEEA"
     gene            2521..2595
                     /locus_tag="PK077_08585"
     tRNA            2521..2595
                     /locus_tag="PK077_08585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2553..2555,aa:Val,seq:cac)
     gene            2696..>3581
                     /locus_tag="PK077_08590"
     CDS             2696..>3581
                     /locus_tag="PK077_08590"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRN24394.1"
                     /translation="MLCAVGIDLVFLSPYASAHMPALLLTSFIWALSFGLLGNCLSGL
                     PASFVALARMAGSFAVFLPFVRKAPLSHAVGMMGIGAVQFGGMYLFYIAAFHYLPSHQ
                     VALFTATTPIYVVLIDGLMKKNLPPLHLAAAILAAMGGAGILWKGYSSGNGELTGIVL
                     VQLSNLCFAAGQIAYISLKNSWFGRSEASCFAYAYAGALLVTLPSGLPSGLACWQGIS
                     TVQWWLLAYLGVIASGVCFFLWNYGARRVTPVKLAIMNNLKIPLAAMTSLLLFREHSN
                     MFLLSIGCLLILASFLIVP"
BASE COUNT          738 a         1087 c          900 g          846 t
ORIGIN      
        1 aaatatcccc ggaccacttc gtggaagcct gcccccgttg cgccaacctt taacttcttt
       61 ttctaccagc catgccggaa cctctcttgc aagtagacaa tttgaaaatg tacttccccg
      121 tccgctccgg aattttcctg cggcaagcgg gatgggtcaa ggctgtggac gacgtttcct
      181 tcaatattta cccgggggaa acgctgggcc tggtggggga atccggctgc ggaaaatcaa
      241 ccattggcaa aagcattgtc cgcctgctga aacccaccgg gggctccatc cggttcaacg
      301 gcaacaatat cgccagactc tcccagcgca aaatgaggcc tctacgtccg cacatccaga
      361 tggtcttcca ggaccctgcg gaatccctca accagaggca atccatcggt caaatcgtag
      421 cggaaccctt tgtcatccac cgcatgggca cccccgccgc gcgccgggaa tgggtgcgcg
      481 gcctgctgga tcgcgtaggg ttgccggaca gcgctattga ccgcttcccc tttgaattct
      541 caggcgggca acgccagcgc atcggcatcg cccgcgccct gacgttaaat cccagcctca
      601 ttattctgga cgaacccgtc tccgcccttg acgtatccgt ccagtcccag gtgctcaacc
      661 tgctgctgga acttcaggag gaacgcaaac tctcttacct gtttattgcc catgatctgg
      721 cggtcgtcaa acacatctcc gaccgcgtgg cggtcatgta tctgggtaaa attgtggaaa
      781 tgtcacatgc ggaaaccatc tatcaatccc ccaaacatgc ttacaccaag gctcttctgg
      841 acgctattcc ggaaccagac ccctccaggg ccaacaagca ccagcctctg ccgggagatg
      901 tcccctctcc catcaacccg ccgctcggct ctgctttcgg ccaccgcatc cagcacccct
      961 cctatccgga gacggtggga gcggacctta cccctgtgga aatagagccg ggccactggg
     1021 tagcccctga tccctgcgct ctggagccgg aagactggaa tagaatccgc cagcggtaaa
     1081 agtacggtaa aacgtttatc cgtgtccgcc ggggaaaaac atatctgcaa ataaatcccc
     1141 tgattttttt ccgggcggcg ggcaacagct tttaatcacc atgcccccgg cattatccta
     1201 cacgtaaatc cggggcacgc gggcgctgat gctggtcagc acattgctgg ggatggtatt
     1261 ggcacggcgc gtcagttcct cccagcttac attaggcccc atgatttcca ccaggtctcc
     1321 ggtctccaca tggtccatgt aagtaacgtc caccatgatc tggtccatcg tcacacggcc
     1381 caaaaccgga cagtactggc cattgatata caccctcgct ccctggttgg acagatattg
     1441 aagatagccg tccgcataac cgatgccgat agtggccacc ttcgtgctcc ccttcgttat
     1501 atagcagtgg ttgtaggaaa cgccgtgatt tcccggcagc gtgcgtatca gagtaatgcg
     1561 gctgtacagg gtcattgtag gccgcagctc tttattatag ggagacggca tgggggaata
     1621 cccgtacagg attctgccca gcctcgccat attggcgcag ggaaccttgt aattaaacac
     1681 agcggcgctg ctgcacaggt gccggaactt gtaccgatgg ccttgggaaa gttcatttac
     1741 ggcttccgta aagccgctga tctgtccgtg ggtgaaggaa atatcatcgg aagctgcgga
     1801 taaatgggaa aacacgccct ccagatccag ataactgaac tgtttgagct tgtccgtcag
     1861 gccgtgcaat tcactcggta aaaaaccgct gcgccccatg ccggtatcca cgcccaaatg
     1921 gacatggacg gttttgccat aaagggcgcc cagggaattg aaatgctccn nnnnnnnnng
     1981 cctcttccat gctggaaaga gcggcgcgcc agccgttctg cacgatttcc tcccgctccc
     2041 cctcaaaaca ggggccgaga ataaacgggc atgtttttgc cccggcatcc ctcacccggc
     2101 aggcctccgc caccgtggca acgccgaaaa aagcgcaatc ttcgccatcc agggccttca
     2161 ccaccccttc cagaccgtgg ccataagcct ccgccttcac aatagccatc agacggtgat
     2221 ccggcatgac gcgccggacg acgtttaaat tgtgcctcaa cgcattggta tcaatctggg
     2281 cccatgctct gggagggctt gtgcaactca tgcccggagt gtaggaagac ggttgatgct
     2341 gtacaagccg gaattaagcc acctttccac ttttccaaac cgtccctcgg agaaactgta
     2401 catcggtcat tcctccggga acttgttgac ggcggaaaag aggaatcctt ttttacccga
     2461 tttcaaaaaa ctccttgcca atccccgaca cgaacgatat attccccccg cactgcaaat
     2521 gcgcggttag ctcagtggta gagcacatgc ttcacacgca tggggtcact ggttcgaaac
     2581 cagtaccgcg taccattttc ctgaacaccc cggaattcaa tatttcgggg tgtttttatg
     2641 tttagaacca ttatcttacc ccgttttttg ggtaattttg catgttcccg actatatgct
     2701 ctgcgccgtg ggcattgact tggttttcct ctctccgtat gcttccgccc acatgcctgc
     2761 gttgctgctc acgtctttca tttgggcgtt gtcattcgga ctgctgggga attgcctgtc
     2821 cggacttccc gccagctttg tcgcactggc acgcatggcc ggttcatttg ccgtttttct
     2881 ccccttcgtc cggaaggcgc ccctcagcca tgccgtgggg atgatgggga taggcgccgt
     2941 ccagttcgga gggatgtatc ttttctatat cgctgccttc cactatctgc cttcccacca
     3001 ggttgccctt tttacagcca ccacgccgat ttatgtggtt ctaatagacg gcctgatgaa
     3061 aaagaatctt ccgccgctcc atctggcggc ggctattctt gcagccatgg gaggggcggg
     3121 aatcctctgg aaaggatatt cctccggtaa cggggagttg acaggaatcg ttctggtcca
     3181 gctttccaac ctctgcttcg cggcaggtca gatagcctac atttccctga aaaattcctg
     3241 gttcgggaga agcgaagcat cctgcttcgc ctatgcctat gcaggcgccc tgctggttac
     3301 tttgccttcc ggcctgccgt cgggcttggc ctgctggcag gggatttcaa ccgtgcaatg
     3361 gtggctgctg gcctatttgg gcgtcatcgc ttcaggagtc tgttttttcc tgtggaatta
     3421 cggcgcgcgc cgggtaactc ccgtaaagct ggcgattatg aacaatctca agattcccct
     3481 ggcggccatg acttccctgc tactgttcag ggagcattcc aacatgttcc ttctgtccat
     3541 cggctgcctg cttatcctgg catctttcct gatcgtccct a
//
LOCUS       DAOMSU010000222         3578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2585_length_3578_cov_3.150724, whole genome shotgun sequence.
ACCESSION   DAOMSU010000222 DAOMSU010000000
VERSION     DAOMSU010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2585_length_3578_cov_3.150724"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="PK077_08595"
     CDS             complement(<1..437)
                     /locus_tag="PK077_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24395.1"
                     /translation="MLNTASGENNQFLKKWEISPSYLKELESSSSGLKLFSSEERKAL
                     YHYINQDYADKFRLMAGFLKCSQEGEEGNPAIDLVDPQFLQGLKGVVEKGINSDWFPK
                     LPIFYQKQIKIQYEELLKITQNKSKQVTLDIAEKLLDNSYGSR"
     gene            complement(469..>3578)
                     /locus_tag="PK077_08600"
     CDS             complement(469..>3578)
                     /locus_tag="PK077_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRN24396.1"
                     /translation="HADGTVLEQSGTGQRHLLYRTEYSAEGVVRSTLLPRAEGEPALV
                     EQTVTDGRGNMVRVSRANANGGLIHDRRAFDLNNRLLRQQVDGMAPLLYDYDPFGNIV
                     KTTLKLAENPTSANSLVTEYAYARRQREDGVYRVTTVTRCNSQGTTYAESTAELVSFL
                     SPSLAGKNISTDPRGNETLQWTEYAAPARRTVKTQSPASSVIAETVVIDGYAVSRKDH
                     AGVLTASSRAYTASGSTETYTDARGNAAVTVFDIAGRETARTDAAGNTTAIQYDPSTA
                     SPSCVTDALGNTACYAYDPRGRKTAEYGTALQPSVFGYDDADRLVSLMTFRVPGETIA
                     ADPRERTDGDVTAWSYDDASGLMTAKTYADGHGESYSYDDWNRLAVKRQARTVDGQGT
                     PLATSYAYDSQTGNLVSVTHNDATPSITCTYNHLNLLTQVTDDSGTRTLAYNQYNEAE
                     SETAAGLAASALNYLRDGLERPSGYSLHYGEGIVQQTAWEYDGCGRLSTVSLNNGSDP
                     FVYGYHAVNGLLETLDYPNTLRRWYTREEKRDLLTRIDYMRPGSVNYPAKTDYAYDAL
                     GRPTEKKDYFNTPAPDLTHSYSYNGRGELAADAMSRGGTYSYVYDNIGNRVTSRGGSG
                     ASAAAYTANSLNQYTAITREEEAPFAPGYDADGNQTKIQTSTGEWEVSYNALNQAARF
                     IQGNRRVECRYDYLNRRIEKAVYEGEVLMSKKRFIYHGYLQIAELDAADATESAMPVL
                     RKTYLWDPLEPVATRILAMSVFDETGTWAEDLYCTHDLLKNTTALFGIRAGRRALYEY
                     GPYGNILRMEGNAAEDNPFRFSSEYADDELGLVYYNYRYYNPQNGRWVSRDPIGEFYA
                     RNLYEFVNNNPLIRFDYIGLDMIPWAIDPSVISQAGFAWADLQRQLEEAAKTEEQRKK
                     EKEERKKQYDECVKRCDDQRQASSEELNRKTEDTHSKAWEDMMKTSKECDESRWDRAI
                     KLLSESLGAAQACSPVNGPPIRYNPDCLGSSIPKGVGQFITVASIQAKYNECVENCKK
                     QFPHV"
BASE COUNT          733 a          969 c          927 g          949 t
ORIGIN      
        1 cttctacttc cataggaatt gtcaagcaac ttttcagcta tatctagagt tacttgctta
       61 ctcttattct gcgtaatttt caacaactcc tcatattgaa tctttatttg cttttgataa
      121 aaaataggta atttggggaa ccagtcagaa ttaatacctt tctccaccac accttttaaa
      181 ccctgaagaa attggggatc gaccaaatct atcgcgggat ttccttcctc tccttcttga
      241 gagcatttca aaaaaccagc catcaaacgg aatttatcag cataatcctg attaatgtag
      301 tgataaaggg ctttcctctc ttcagaagaa aataatttta atccggaaga agaagactcc
      361 agttctttca aataagaagg gcttatctcc cattttttca aaaactggtt gttttctcct
      421 gatgctgtgt tcaacataca gctggctata cacgaaatgg ttaatatttt atacatgggg
      481 aaattgtttt ttacagtttt ctacacattc attgtattta gcctgaatag atgccacagt
      541 tatgaattga ccgacccctt ttggtatcga agatcctaga caatcaggat tgtatcgtat
      601 aggggggcca ttgactggac tacaagcctg agccgccccc aaactttcac tcaataattt
      661 aatcgctcta tcccatcgcg attcatcaca ttctttagaa gttttcatca tatcctccca
      721 cgctttggaa tgagtatctt cagtttttct attcagttct tctgaacttg cctgtctttg
      781 gtcatcacat cgcttcacac attcatcata ttgtttttta cgttcttctt tttctttttt
      841 cctttgttct tctgttttgg cagcttcctc caattgcctt tgcaaatctg cccaagcaaa
      901 accagcttgc gatattacag atggatcaat tgcccaagga atcatatcca accctatata
      961 atcaaatcta attaggggat tattatttac gaattcatag agatttcttg cgtagaattc
     1021 tccaatggga tccctgctta cccacctgcc attttgggga ttataatagc ggtaattgta
     1081 gtaaaccagc cccagttcgt catcagcgta ttcgctggag aaccggaacg gattgtcctc
     1141 tgcggcattc ccttccatcc tgagaatatt cccatacggg ccgtattcgt acaaggcgcg
     1201 gcgtcccgct cggatgccga agagcgccgt agtgtttttc aacaggtcgt gcgtgcagta
     1261 caggtcttcc gcccaggttc ccgtctcatc aaagacactc atggccagga tgcgtgtggc
     1321 taccggttcc agaggatccc acagataggt ttttcgcagt acgggcatcg ctgattccgt
     1381 cgcgtcggca gcatccagtt ccgcgatttg caggtagccg tgataaatga atcgtttttt
     1441 cgacatcagg acttctcctt catagacggc tttctcaatc cgcctgttca gatagtcgta
     1501 gcggcactcc acccgcctgt tcccctgaat aaaccttgcc gcctggttca gggcattata
     1561 agaaacttcc cattctcctg tggacgtttg aatctttgtc tggttgccgt cggcatcata
     1621 gccaggcgcg aaaggcgctt cttcctcccg ggtgatggcc gtgtactggt tcaggctgtt
     1681 ggccgtgtac gccgccgctg acgcacccga acctccccgg gaggtgacgc ggttgccgat
     1741 gttgtcatac acataggaat atgttccccc ccggctcatc gcgtcggcgg ccagttcgcc
     1801 gcggccgttg tagctgtagc tgtgcgtcag gtcaggagca ggggtattga agtagtcctt
     1861 cttttctgtg ggccgcccca gcgcgtcata ggcgtagtcg gttttggccg ggtagttgac
     1921 gctgccggga cgcatatagt cgattctggt cagcagatcc cgtttttctt cccgggtgta
     1981 ccatctccgg agggtattgg ggtaatcaag cgtttccagc agtccgttga cggcgtggta
     2041 accatagacg aagggatcgg agccgttatt gagcgagacc gtagaaagac gtccgcaacc
     2101 gtcatattcc caggccgtct gctggacaat gccctccccg taatgcagac tgtaacccga
     2161 aggccgctcc aaaccgtcgc gcagatagtt gagcgcgctt gccgccagcc ctgccgcagt
     2221 ttccgattcc gcttcattat attggttgta agccagcgtc ctcgtgccgg aatcgtccgt
     2281 gacctgcgtg agcaggttaa ggtgattgta ggtacaggtg atggaaggag tcgcgtcgtt
     2341 gtgagtgacg gagaccaggt tgcccgtctg cgaatcgtag gcataagaag tcgccagagg
     2401 cgttccctgc ccgtccaccg tccgggcctg tcgtttaacc gccagcctgt tccagtcatc
     2461 gtaagagtag ctttccccat ggccgtcggc ataggtttta gccgtcatca ggccggaggc
     2521 gtcgtcatag ctccacgccg tcacgtcccc gtccgtccgt tcccgcggat cggcagcgat
     2581 ggtttccccc ggaacgcgga acgtcatgag ggataccagc ctgtccgcat catcgtagcc
     2641 aaagacggag ggctgaaggg ccgtaccgta ttcggcggtt ttgcgtcccc gcgggtcgta
     2701 agcgtagcag gccgtgttgc ccaaggcatc cgtgacgcag gagggggaag ccgtggacgg
     2761 gtcatactgg atggcggtcg tattgccggc ggcgtccgtc ctggctgttt cacggccggc
     2821 gatgtcaaag acggtaacgg cggcattgcc gcgggcgtcc gtataagtct ccgtgctgcc
     2881 gctggccgta taagcgcggg aagaggcggt cagaactccc gcatggtctt tccgggatac
     2941 ggcgtaaccg tctatgacga cagtttccgc aatgacggaa gaagccgggg actgcgtttt
     3001 taccgtccgt ctggccggag ccgcgtattc cgtccattgc agggtttcat tgccccgcgg
     3061 atcggtggag atgttttttc cggccagcga gggggacagg aaggaaacca gctcggccgt
     3121 gctttccgca tatgttgttc cctggctgtt gcagcgcgtg actgtggtta cccggtacac
     3181 gccgtcttcc cgctgccggc gggcataggc gtactccgtg actagggagt tggcggatgt
     3241 ggggttttcg gccagtttga gcgtggtttt gacgatattg ccaaacgggt catagtcata
     3301 aagcaacgga gccattccat ccacctgctg ccgcaaaagc ctgttgttca gatcaaaagc
     3361 gcgccggtca tgaataaggc cgccgttggc gttcgcccgg gagacgcgca ccatgttgcc
     3421 cctgccgtcc gtgacggttt gttccaccag cgccggttct ccctccgccc ggggaagcag
     3481 cgtggaacgg accacgcctt ccgcggagta ttccgtacgg tagaggagat gcctctgccc
     3541 cgtgccgctc tgttccagca cggtcccgtc cgcatggc
//
LOCUS       DAOMSU010000223         3574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2594_length_3574_cov_2.530264, whole genome shotgun sequence.
ACCESSION   DAOMSU010000223 DAOMSU010000000
VERSION     DAOMSU010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2594_length_3574_cov_2.530264"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            158..1177
                     /locus_tag="PK077_08605"
     CDS             158..1177
                     /locus_tag="PK077_08605"
                     /inference="COORDINATES: protein motif:HMM:NF015177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HRN24397.1"
                     /translation="MVDLYHKTWEIAAGRVRKGPEGLPASPYMDENCYEDQIWIWDTC
                     FMVLFAKYAPRSFPGIESLDNLYKPIHEKAATPLRIHLVDNPPLFAWVEKEYFDFTGD
                     KGRLNHLLNEKRYLQKHFKWFARAKAGERFECSPQRIYLNSIGDDGFTWTGGASGMDN
                     TPRGRDAGGYHKVLWVDAISQQALSAHCIAAMEQALGNEDEARKWNAEYEALKKKINH
                     LYWDERDGFYYDVTIADKQPCRVKTIASYWPLLAQVASREQARSMVRHLMNPEEFGGS
                     YPTPSLARSDKDYHHQTGDYWRGGIWLPTTYMAIKAIESMATMRRPMLLPRRLSTSSL
                     PLTGI"
     gene            complement(1744..2598)
                     /locus_tag="PK077_08610"
     CDS             complement(1744..2598)
                     /locus_tag="PK077_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HRN24398.1"
                     /translation="MTVRRTTNQGLSFLDVTTPLCTASLCLQGAHLTSWKPAGHEECL
                     FLSPNAAFVPGKAIRGGIPVCWPWFGPREGAPSHGIARTSEWRLHHQEMDKRGNVLLS
                     LAFYPEDESYPAAILRLTLGRTLTMKLETTARTQPCKLTEAFHNYFAVGNLTKCRVHG
                     LEDIPFREEAAQPMKHGERPLAPLGCLDRVYNFPSCSGKTMLEDLMLNRAITVERNHA
                     SSVIVWNPGQQGAKNMADLGAESWNKFLCIETGNAEPRSISLAPGQTHTLYQKISVRH
                     MEEACSPR"
     gene            complement(2595..3233)
                     /gene="pdxH"
                     /locus_tag="PK077_08615"
     CDS             complement(2595..3233)
                     /gene="pdxH"
                     /locus_tag="PK077_08615"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HRN24399.1"
                     /translation="MDLSNFREEYLKGQLHRKDLAENPFEQFQTWFEQALKADIPEPN
                     AFSLATADAQGRPSLRTVLLKYFDRTGFVFFTNYGSHKARDIEENPQVCMMLPWVMLE
                     RQIIIYGKAVKVSRAESLKYFLTRPKESQLGAWVSHQSSVISGRKLLEMKLMELKNKF
                     SKGEIPLPSFWGGYRIIPDTFEFWQGGPGRVHDRFMYKLQENGSWTIERLQP"
     gene            complement(3255..>3574)
                     /locus_tag="PK077_08620"
     CDS             complement(3255..>3574)
                     /locus_tag="PK077_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HRN24400.1"
                     /translation="ECINRLRQEKPGVEIQLEADTLEQVGNFLKLEGVDHILLDNMTP
                     EMLKQAVAMRGGRTTPLLEASGGVHLDTVAAIAESGVDFVSVGYVTHSAPSLDLGLDF
                     SVE"
BASE COUNT          801 a          841 c         1062 g          870 t
ORIGIN      
        1 aaaaataatg atgagcgcta aactatcggt aattacaggt tcgattctcc tgctgtcagc
       61 cccgttgcag ggattttccg cgacaaccca tccggattcc tgcatgaacc gggattggaa
      121 aaaagagatt ccccttccgg tgtatccaaa ccgggaaatg gtggatcttt atcataagac
      181 atgggaaatt gcggcaggcc gcgtcagaaa agggccggaa ggccttcccg cgtctcccta
      241 catggatgag aattgctatg aagatcaaat atggatatgg gatacctgtt tcatggtgct
      301 ttttgccaag tatgcgccta gatcgtttcc cggaatagaa agcctggata atctttacaa
      361 acctatccat gaaaaagccg ctacgcccct tagaatccat ttagtggata atcccccgct
      421 gtttgcttgg gtggaaaagg aatattttga tttcacagga gataaggggc ggcttaatca
      481 tcttcttaat gaaaaacggt atttgcagaa gcatttcaaa tggtttgccc gggctaaggc
      541 tggtgaacgg tttgagtgtt ccccccagcg tatttacttg aattccattg gggatgatgg
      601 ctttacctgg acagggggag cgagcggtat ggacaatact ccccgtgggc gtgatgccgg
      661 aggataccat aaggtgttat gggtggatgc catttcccag caggctctta gcgcccactg
      721 cattgctgcc atggaacagg ctttgggaaa tgaagatgaa gcaaggaaat ggaacgctga
      781 atatgaagcg cttaagaaaa agatcaacca tctttactgg gatgagcggg atggatttta
      841 ttacgatgtc accattgcgg acaaacagcc ctgccgcgtt aaaaccattg cgtcctactg
      901 gcctcttctg gctcaggttg cctccaggga acaggcgcgg agcatggtca ggcatttgat
      961 gaatcccgaa gaattcggag gcagttatcc gactccttcc ctggcccgct cggataagga
     1021 ttatcatcat caaaccgggg attactggcg gggaggaatc tggctgccga cgacatatat
     1081 ggcgattaag gccattgaaa gtatggctac catgaggagg ccgatgctat tgccgagaag
     1141 gttatcaacc agcagcttgc cgcttacagg aatatagaac cgcatactat ctgggagtgc
     1201 tacagcccaa gcgggaatac tccttccacg gaacacggac gccgcgcaag accggaattt
     1261 tgcggctggt cagccctggg gccgatttcc ctgtttattg aaaatgtact gggatttaag
     1321 gaggtgtccg caaccagaaa agaagtgcgg tggaggttga agaaaaataa gggccgccat
     1381 ggaatcagga atttgaggtt tggcgatatt gtaaccgata ttgtttttga tggtaaaggc
     1441 acggtgtcgg tcacgtcgaa tgcttcttac tctctaatca ttaatggcaa tacttattca
     1501 gtaaggaagg gggatactga aattaagctg taaggacctt ctgtttatcc acggaagggc
     1561 gtcattgatg ttcttccgcg gggtttttcc ttttgccggg gcaagatgcg ggtttttccc
     1621 gtgcctgcgc caattttttt cttttggagg cggtctgaga ggcaagggtg acaaaggaaa
     1681 ttccggaagg gcggcaagga aatgttccgg aacattattt gagaggtaat gttgtctgcc
     1741 gttttaacgg ggagagcagg cttcctccat atgccttacg gaaatttttt ggtaaagggt
     1801 atgggtttgt ccgggggcga gggaaatgga acggggctcc gcgtttcccg tttcaatgca
     1861 caggaattta ttccaggact ccgcacccag gtccgccatg tttttggcgc cctgttgtcc
     1921 aggattccag acgatgacgg aagaggcatg attccgctcc accgtgatgg cgcggttcag
     1981 catgaggtct tccagcattg tttttcccga acaggagggg aagttgtaaa cgcggtccag
     2041 acaccccagc ggcgccagcg ggcgttctcc gtgcttcatg ggctgggcgg cttcttcccg
     2101 gaacgggatg tcctccagcc catgaacgcg gcatttggtc aggttgccga cggcaaagta
     2161 gttgtggaag gcttccgtga gcttgcaggg ctgcgtgcgc gccgtggttt ccagcttcat
     2221 ggtcagcgtg cggcccaggg taagacgcag gatggcggcg ggataggatt catcttccgg
     2281 atagaaagcc agggagagca ggacgttgcc gcgcttgtcc atttcctggt ggtggaggcg
     2341 ccattccgag gtgcgcgcta tgccgtggga cggcgcgcct tcccggggac cgaaccaggg
     2401 ccagcagacg gggatgcctc cgcggatggc tttgccgggg acaaaggctg cgttgggaga
     2461 aagaaaaagg cattcctcat gccctgccgg cttccatgag gtgaggtgcg ctccctgaag
     2521 gcacagggat gccgtgcaga gcggtgttgt tacgtccagg aaagaaagcc cctggtttgt
     2581 tgtgcgccgg acggtcatgg ctgaaggcgt tcaattgtcc aggagccgtt ttcctgaagt
     2641 ttgtacatga aacggtcatg cacgcggccg gggccgccct gccagaattc aaaggtatcc
     2701 gggataattc ggtagccgcc ccagaaggag ggaaggggta tttctccctt ggagaatttg
     2761 tttttcagtt ccatcaactt catttccaac agttttcttc cggaaatgac ggagctttgg
     2821 tgtgaaaccc aagctccaag ctgggattcc ttggggcggg tgaggaaata tttcagggat
     2881 tccgcgcggg agactttgac ggcttttccg taaatgatga tctggcgttc cagcattacc
     2941 cacggaagca tcatgcagac ctgtggattt tcttcaatat cgcgggcctt gtggctgccg
     3001 tagttggtga agaaaacaaa gcccgtccgg tcaaaatatt tgagcagcac ggtgcgtagg
     3061 gagggcctgc cctgggcgtc cgccgtggcc agggaaaaag cgttgggttc cggaatatcc
     3121 gctttcaggg cctgttcaaa ccaggtttgg aattgttcaa agggattttc cgccaggtcc
     3181 tttctgtgca gctggccttt gaggtattct tctctgaaat tggacaagtc catggcgtgt
     3241 tgaacgatga ggggttattc tacgctgaaa tccagcccca ggtccaggga gggggccgag
     3301 tgggtcacgt agcctacgga gacgaagtcc acgccggatt ccgcaatggc ggctaccgta
     3361 tccagatgaa cgcctccgct ggcttccagc aggggggtgg tgcgtcctcc gcgcatggcg
     3421 acggcttgtt tcagcatttc cggcgtcatg ttgtccagaa ggatatggtc cacgccttcc
     3481 agtttaagga agttgcccac ctgttccagg gtgtccgctt ccagctggat ttccacaccg
     3541 ggcttttcct gacgcagcct gttgatgcat tcct
//
LOCUS       DAOMSU010000224         3565 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2601_length_3565_cov_2.433903, whole genome shotgun sequence.
ACCESSION   DAOMSU010000224 DAOMSU010000000
VERSION     DAOMSU010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2601_length_3565_cov_2.433903"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1697)
                     /locus_tag="PK077_08625"
     CDS             complement(<1..1697)
                     /locus_tag="PK077_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRN24401.1"
                     /translation="MTFLYPNVLYALILPALLAAAAWWLWRRRSRKWEVLVSPEYRRE
                     LVNAPATWHRVLPVIFAVLASIFAILSMARPVDGYTEVKEIPKSRNILIAIDCSRSML
                     SKDASPTRLGRAKTAAYDLLDALPGDNFGIIIFSGDAVLLMPLTHDHNALKETIEQLQ
                     FGWVSQGGTNLENVVRLALQTFKRDKEADAKNALVILSDGEDTVNITYKTAEAARQHQ
                     LIIVTAGIGTTIGTTIPDEQSPSGLYRDRRGQHVVSKLNPESLQYLARQTEGQYVQLS
                     DGAALNRFVKDIADRLDTTEGKEEVRRVPNDRYIIFAVPALICLLLTLLAGTRWRSFR
                     RSGRRGMTSLTAAALFCAGLLGQEAQADTHALDNVTDLIRTGKTEEAVKSIDEMLAVP
                     DLAEETRQALEFAKGCLEQKADNPKEAAEAFSQALLSPKPALQADSHFNLGNLEAAQA
                     RKTMTFSRPEGEQQQARPSSIDDQIKEIDARLAKIPVAKKHVKEAVKRFDDALSAYGS
                     HEGAASNKEEMLRYDKSLDEYRRQLEELKKKLEEEKKKQQDQNKDQNKDQNKDQNKD"
     gene            complement(1716..2702)
                     /locus_tag="PK077_08630"
     CDS             complement(1716..2702)
                     /locus_tag="PK077_08630"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRN24402.1"
                     /translation="MTEHFQFAQPEWLYALPFILCLIILRRRRGAEGSITYPTVRFIA
                     SLARTPQSLAGKIGALCFVLAAAAIAIALARPQHVEDKTFRTVNGIDIMIAFDLSYSM
                     ETPDMVLNRMPINRLVAAKHVITQFVDSRPDDRIGIVGFAGKTKSFCPLTLDHALVNS
                     IIRDFHPRMIQADGTAIGSAIAAAATRLDDRKNTKSKIIILVTDGASNSGQISPLVAA
                     ENAAKLGIKIYTIAVGTEEGTLANGMVVQSEFDEPTLRKIAQLTGGEHFRATNMASFN
                     KAFTSIGKLEKSEAKVQTVRHIEEYFMYFLVTGAALALLGLSLQVLKPAPAP"
     gene            complement(2709..3254)
                     /locus_tag="PK077_08635"
     CDS             complement(2709..3254)
                     /locus_tag="PK077_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24403.1"
                     /translation="METQSPDAYFQAGFWGAWGIWIILASILLAVAIAVIVLIRRKGR
                     IPAAPLSPSETAIRDISMLRNTHPSLRQAAVEFSLILRKYLVGETKDPALYETQQEFN
                     RRADALTALPSELQAPTRDLLDRMASLKYEPDTPENDSMVNELADATVQLINDIEADA
                     HRTKEETDLVVKKSSSRFNQR"
     gene            3347..>3565
                     /locus_tag="PK077_08640"
     CDS             3347..>3565
                     /locus_tag="PK077_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24404.1"
                     /translation="MKIYGIFWQKGPEGRHEVVRGLQVQEVNAQATEILRPFLAVQMN
                     DVGVGFPDILTGKVQEHAFASFRIFQDDA"
BASE COUNT          663 a          892 c         1167 g          843 t
ORIGIN      
        1 tggtccttgt tctggtcctt gttctggtcc ttgttctggt ccttgttctg gtcctgttgt
       61 tttttcttct cttcttccaa tttcttcttt aattcttcca gctgccggcg gtattcgtcc
      121 agggatttat cataacggag catttcctcc ttgttggacg ctgctccttc atgggaacca
      181 taggcagaga gcgcatcgtc aaaacgtttg acggcctctt taacatgttt tttcgcaacg
      241 ggtatttttg ccagccgggc atcaatttcc ttaatttggt cgtcaatgga agagggccgg
      301 gcttgctgct gttccccttc cggcctggag aaagtcatgg tcttgcgggc ctgggcggct
      361 tccaggttgc caaggttgaa gtgggaatcc gcctggaggg ctggtttggg agaaaggagc
      421 gcctgggaga aagcttccgc ggcttctttg gggttatccg ctttttgttc caggcatcct
      481 ttggcgaatt ccaaggcctg gcgcgtttct tccgccaggt cgggaacagc gagcatttca
      541 tcaatggatt tgacggcttc ttccgttttt cccgtccgga tcaaatccgt gacattgtcc
      601 agggcgtgcg tgtccgcctg cgcttcctgg cccagcaatc cggcgcagaa taacgccgca
      661 gcagtcagag atgtcatacc gcggcgtccg gaacggcgga aggaacgcca gcgtgttccc
      721 gccagaaggg tgaggagcag gcagatgagg gccggaacgg caaagatgat gtagcggtca
      781 ttcggcacgc ggcgcacttc ttccttgcct tccgtggtat ccagccggtc cgcaatgtcc
      841 ttgacgaagc ggttcagcgc cgctccgtcg gagagttgga catattggcc ttccgtttgc
      901 ctggccaagt attgcaggct ttccggattg agcttggaga cgacgtgctg gccacgacga
      961 tcccggtaca ggccggaggg ggattgctca tccggaatag tggtgccgat agtagtaccg
     1021 atacccgctg tcacgatgat aagctggtgc tgccgtgcgg cttccgcagt cttataggtg
     1081 atgttgacgg tgtcttcccc gtcgctgagg atgaccaggg cgtttttggc gtccgcttcc
     1141 ttgtcccgtt tgaaggtttg aagggccagc cgcacaacgt tttccaggtt ggttccccct
     1201 tgggagaccc agccgaattg aagttgttca atggtttctt ttaatgcgtt atggtcatgg
     1261 gtaaggggca tgagcaggac ggcgtccccg gagaagatga tgatgccgaa gttgtccccc
     1321 ggcagggcgt ccagcaggtc gtaggcggcg gtttttgccc ttcccaggcg ggtcggggaa
     1381 gcatccttgc tgagcatgga acgggagcag tcaatagcaa tgaggatgtt gcgcgatttg
     1441 gggatttcct ttacctccgt gtagccgtcc accggacgcg ccatggagag gatggcgaag
     1501 atggacgcca gcacggcaaa gatgacgggg agcacccggt gccaggtggc cggagcgtta
     1561 accagttccc gccggtattc cggcgaaacg agcacttccc attttctgga acggcggcgc
     1621 cacagccacc aggccgccgc cgcgagcagg gccgggagaa tgagcgcgta cagaacgttg
     1681 ggataaagga aagtcatgac gggtgaagat cggtattacg gggcgggggc cggtttgagc
     1741 acttgcaggg agaggcccag aagcgccagc gcggctcccg ttacaaggaa gtacatgaag
     1801 tattcttcaa tgtggcggac agtttgcact ttggcctcgc ttttttccag cttgccgatg
     1861 gaggtgaacg ctttattgaa ggaggccata ttggtggccc tgaagtgttc tccgcccgtg
     1921 agctgggcta ttttccgcag ggttggttcg tcaaattcac tttgcaccac catgccgttc
     1981 gccagggttc cttcttccgt gcctacggca atggtataaa ttttgatacc cagcttggcc
     2041 gcgttttccg ccgccaccag cggggatatc tggccggagt tggaggcacc atccgtcacc
     2101 aggatgatga tttttgattt ggtgttctta cggtcgtcca gccgggtggc cgccgccgca
     2161 atggcggagc cgatggccgt gccgtccgcc tggatcatgc gcggatggaa atcccggatg
     2221 atgctgttga ccagggcgtg gtccagcgtg agcggacaga aggattttgt ttttccggca
     2281 aatcccacga tgccgatgcg gtcgtccgga cggctgtcaa caaattgggt gatgacgtgt
     2341 ttggcggcta ccagccggtt gatgggcatt cggttgagaa ccatgtccgg cgtttccatg
     2401 gagtaggaaa ggtcaaaggc gatcatgatg tcaatgccgt tgactgtccg gaaggtttta
     2461 tcttccacgt gctgggggcg cgccagggcg atggcgatgg cggcagccgc cagaacgaag
     2521 caaagggccc ctatttttcc tgccagggat tgcggagtac gcgccaggga agcgatgaag
     2581 cgcacggtgg ggtaggtgat ggagccttcc gccccgcgcc tgcggcggag gatgatgagg
     2641 cacaagatga agggtaaggc ataaagccat tcaggttgtg caaattggaa gtgttccgtc
     2701 atggtatgct agcgttggtt gaagcgggat gaagattttt tgacgacaag gtccgtttct
     2761 tccttcgtcc ggtgggcatc cgcttctata tcattgatga gttggaccgt agcgtccgcc
     2821 aattcgttga ccatggaatc gttttccggg gtgtccggtt catatttgag agaagccatg
     2881 cggtccagca gatcgcgcgt gggggcctgg agttcggaag gcagcgcggt gagcgcgtcc
     2941 gcccggcggt tgaattcctg ctgggtttca tatagtgccg ggtctttggt ttctcctacc
     3001 aggtatttgc ggaggatcag ggaaaattcc acggcggcct gcctgaggga gggatgcgtg
     3061 ttccggagca tcgagatgtc ccggatagcg gtttccgacg ggctgagcgg cgcggcgggg
     3121 atgcgtccct tcctgcggat gagcacgatg acggcgatag cgacggccag aaggatggag
     3181 gccaaaataa tccagatgcc ccacgcgccc caaaagcccg cttggaagta ggcatccggg
     3241 gattgtgttt ccatcaggga gggaaacgcc ggcggttccg gaattgccgt gggggggggc
     3301 tgaaaggtgg cgggggttgg catggctggc ggagaagagg attagcgtga aaatttacgg
     3361 gatcttctgg caaaagggcc cggaggggag gcatgaagtt gtccgtggtc tgcaagtcca
     3421 ggaagtcaat gcccaggcga cggaaatcct gcgtccattt ctcgcggtgc agatgaacga
     3481 cgttggcgtg ggtttcccgg acatcctgac gggaaaggtt caggaacatg cttttgccag
     3541 tttccggatc tttcaggatg acgcg
//
LOCUS       DAOMSU010000225         3560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2606_length_3560_cov_2.554922, whole genome shotgun sequence.
ACCESSION   DAOMSU010000225 DAOMSU010000000
VERSION     DAOMSU010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2606_length_3560_cov_2.554922"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PK077_08645"
     CDS             <1..309
                     /locus_tag="PK077_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24405.1"
                     /translation="AAASSGWSGMFAAALVLQPLLPFLILLFPALRRRPSALGAVCAG
                     VLFGSLLEACWMFGPGLGLDPSAWKAWLPLVLLLGGMALICCFSFLGALSVRRVFPES
                     "
     gene            306..2633
                     /locus_tag="PK077_08650"
     CDS             306..2633
                     /locus_tag="PK077_08650"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HRN24406.1"
                     /translation="MMNRTAAVFFLFSRRPGEYLPLFWLAGAFVLGFLWSLAYASGLV
                     AEEPLPWPAGRLMFAFYGMLVYGWALPVLFSFAAGSRSRWMKPVEWLWHACVALGLAT
                     IAAGNGSGVLLMPFDWWVCPVFAFLAAGAACVAWFRPFPWTVRLLFSSSAGCVAAALW
                     ALGHTQALSMNGLLDVSALGGSLSCGLMFAALGAAALRLDMALGRAAGILSVWMALVL
                     AAAPVIGGLGGLQGVPVPWMLVEAGTAWFWCAVLPAVLAVARFWIKSGTKTGIWVKTG
                     VSLLALLAAWNVFVVGAPELMQFSLSAWHEPELWILLGAGVLMMAGGKAPGHFPVAWR
                     LLGAGVCGVLLAYAVGVLAALDVQAFPEARRAELMSGWVGMAACIHAVAMALCLAGSL
                     CLWRPAGAEDGGREPSPPFLLRFFFISAVAAALALATAAAVVLHAPPPDSMEVRRPVH
                     DAEGSGIYAAEGCALCHTQIIRRSLSGKDWQTAIDRGTDPDFPYRVSEPEDMDAEFNR
                     EGAPQAGVAAIGPDLSNAAEYAAGRLEYEDAVSGGTRRAAQVREWLALHLYNPREMQF
                     NKPWTMCPAMPGLFEERPVEGNAPSTAALPVRMERGRELVPSPRGERLLNYLESLRRV
                     EPSLKRDQIHSFPALSHIHPDYAAHPPAVDMERLKKARAAAVMEKGRGVYLSKCAICH
                     GNDGMGDKVTYPPLAGSEWLKEKPDVEIVRIILQGLTGPITVNGKEWDSTMLPPGVTD
                     SRDLANLLTFLRRQFGGVEKVAYTPEQVDAIRRDL"
     gene            2838..3233
                     /locus_tag="PK077_08655"
     CDS             2838..3233
                     /locus_tag="PK077_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24407.1"
                     /translation="MGSTSVGTNSAGTALGDHIKVGNSCVSVGSWSNTDHCYMGIALG
                     YQSNTSNESEKLHTATAYSFCAGMGSRIKGAFCIGTGRGIYVEDGCGHSIVLGGYSRP
                     EVPSGIMISGGHATATRCGSSVRASPAGR"
     gene            complement(3254..>3560)
                     /locus_tag="PK077_08660"
     CDS             complement(3254..>3560)
                     /locus_tag="PK077_08660"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HRN24408.1"
                     /translation="NRLFANSMKKNMNRVTDICLGVIDMKKSLQFYRDKLGFKTDETG
                     DNPDVVFFCTPGTKLELYPLDLLAKDIDPGNPPAPGSGFGGITLAYNAITKEEVVEI"
BASE COUNT          547 a         1013 c         1185 g          815 t
ORIGIN      
        1 gcagccgctt cctccggctg gtccggaatg tttgccgcgg ctcttgttct tcagcctctg
       61 ctgcctttcc ttattttgct gttccccgcg ttgagacgcc ggccgtccgc gttgggcgct
      121 gtatgcgccg gggtgctgtt tggctccctg ctggaagcat gctggatgtt cggccccgga
      181 cttgggctgg acccgtccgc atggaaggca tggcttccgc ttgtcctgct cttgggaggg
      241 atggccctga tctgctgttt ttccttcctg ggggcactga gcgtccgcag ggtatttccc
      301 gaatcatgat gaaccgtacc gccgccgttt tttttctgtt ttcccgccgt ccgggcgagt
      361 atctccccct gttctggctg gcaggggctt tcgtgctggg tttcctgtgg tcgctggcgt
      421 acgcttccgg tctggtggcg gaggagcctt tgccgtggcc ggccggccgc ctcatgttcg
      481 cattctacgg catgctggtg tacggctggg ccctccccgt cctgttttcc tttgcggcgg
      541 gaagcaggag ccggtggatg aaacccgtag aatggctctg gcatgcctgc gttgcgctgg
      601 ggttggcgac tattgccgcg ggaaatggga gcggcgtgct cctgatgcct tttgactggt
      661 gggtttgccc ggtttttgcc ttccttgccg caggggcggc gtgcgtcgca tggttccgtc
      721 cgtttccgtg gactgtacgg ctgttgttca gttcctccgc cgggtgtgtg gccgcggccc
      781 tttgggcgtt gggacatacg caggcattgt ccatgaatgg cctgctggat gtttccgccc
      841 tgggaggaag cctgtcctgc gggctgatgt ttgccgccct gggagcggcg gcgctccgcc
      901 tggatatggc gctggggagg gcagccggaa tcctgtccgt ctggatggcc ctggtgctgg
      961 cggcggctcc ggtgattggc gggctggggg ggcttcaggg ggttcccgtg ccgtggatgt
     1021 tggtggaggc cggtaccgcc tggttttggt gcgccgttct tcccgccgtc ctggcggtgg
     1081 cgcgtttttg gataaaatcc ggtacgaaga ccggaatatg ggtgaagacc ggagtttccc
     1141 tgcttgcctt gctggcggca tggaacgtgt ttgttgtggg tgcgcctgag ctgatgcagt
     1201 tttccctgtc tgcatggcat gaaccggaat tatggattct gctgggggcc ggagttttga
     1261 tgatggcagg ggggaaggcc cccggacatt tccctgtggc atggcggctg ttgggcgcag
     1321 gcgtctgcgg cgtgcttctg gcgtatgccg tgggcgtgct tgccgccctg gatgtccagg
     1381 cttttcctga ggcccgtcgc gcggagctga tgagcggctg ggtgggcatg gctgcctgca
     1441 tccacgccgt tgccatggct ctgtgcctgg cgggaagcct ttgcctgtgg cggccagcgg
     1501 gagcggaaga cggaggccgg gagccttccc ctccgttttt gctgcgtttc ttcttcattt
     1561 cagccgtagc ggcggctttg gcgctggcga cggctgcggc cgttgttctg catgctccgc
     1621 ctccggattc catggaggtc aggagacccg ttcatgatgc ggaaggttcc ggtatttatg
     1681 ccgcggaggg gtgtgccctg tgccataccc agattatccg gcgttccctg tccggaaagg
     1741 actggcagac ggcgattgac cgcggcacgg atcctgattt cccctaccgg gtcagtgaac
     1801 cggaagacat ggatgccgaa ttcaacaggg aaggagcgcc gcaggcgggg gtggcggcca
     1861 taggcccgga cctgagcaat gcggcggaat acgccgccgg gaggctggaa tatgaagacg
     1921 ccgtatccgg cggcacgcgc cgcgccgcgc aggtgcggga atggctggcc ttgcatcttt
     1981 acaacccgcg ggaaatgcag ttcaacaaac cctggaccat gtgtccggcc atgcccggac
     2041 tgtttgagga acgcccggtg gaagggaacg ccccctccac ggctgccctg cctgtccgga
     2101 tggaacgggg ccgggagctg gtgccttccc cgcgcgggga gcgcctgttg aactacctgg
     2161 agtccctgcg gagggtggaa ccctccctga agcgcgacca aatccattcc ttcccggccc
     2221 tgtcccacat tcacccggat tatgccgcgc atcctcccgc cgtggacatg gaacgcctga
     2281 agaaggcccg cgccgccgcc gtgatggaaa agggcagggg cgtgtacctt tccaaatgcg
     2341 ccatctgcca cggcaacgac ggcatggggg acaaggtgac ctatccgccc ctggccgggt
     2401 ccgagtggct gaaggaaaag cctgatgtgg aaatcgtcag gatcatcctc cagggactga
     2461 ccggacccat cacggtgaat ggaaaggaat gggattccac catgctgccg cccggcgtca
     2521 cggattcccg cgacctggcg aacctgctca cattcctgcg ccgccagttc ggcggggtgg
     2581 agaaggtggc ctatacgccg gagcaggtgg acgccatccg ccgggatctg tgacgggagg
     2641 gcccccgatt acaacgtttc atgctccttg ccccgggaac ggaaatggct gcaaatgcca
     2701 ttctatgaat ttagatgata cgtccgccct ttctgcggac agaaacgttg taatcggaac
     2761 aaatgccagt gccgccaatg gcacggacaa tgttgtgata ggttacctct tccaccagag
     2821 acgaccgggg taccgttgtg ggttccacgt ccgtgggcac caactccgcc ggaaccgccc
     2881 tgggggatca tattaaagta gggaattcct gcgtttccgt aggttcctgg tccaatacgg
     2941 accactgtta catggggata gccctgggtt atcagagtaa tacgtccaat gaatcggaga
     3001 agctccatac ggccacggcc tattccttct gcgcgggcat gggttcccgg atcaaggggg
     3061 ccttctgcat aggcactggc agggggattt atgtggaaga cggctgcggg cacagcatcg
     3121 tgcttggtgg ctattccagg ccggaagtgc ccagcggcat tatgatttcc ggcggccacg
     3181 cgacggccac ccggtgtggt tcgtcagtcc gggcgtcacc agcggggaga tgatgctggc
     3241 cagacaagcc cttttagatt tccacaactt cctctttcgt gatggcattg taggccagtg
     3301 ttatccctcc gaagccggaa ccgggggccg gaggattgcc cgggtcaatg tcctttgcca
     3361 gcaaatccag cgggtataat tccagtttgg tgccgggggt gcagaaaaat accacgtcgg
     3421 gattgtcccc ggtctcgtcc gttttaaaac caagcttgtc ccggtaaaat tgcagggact
     3481 ttttcatgtc tatgactcca aggcagatgt cggttactct gttcatgttc ttcttcatgg
     3541 aattggcgaa tagcctgttt
//
LOCUS       DAOMSU010000226         3540 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2626_length_3540_cov_2.955811, whole genome shotgun sequence.
ACCESSION   DAOMSU010000226 DAOMSU010000000
VERSION     DAOMSU010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2626_length_3540_cov_2.955811"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PK077_08665"
     CDS             complement(<1..350)
                     /locus_tag="PK077_08665"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HRN24409.1"
                     /translation="MNQSFEKAERIRACKNRRHVTLVTAYDYPTGRLLDEAGVDIVLV
                     GDSLGMVVLGFPDTTHVTLDHMLHHVEATARGVKNALLVGDMPIHSYDTVEQAVQSAK
                     KLFLAGADAVKLEGG"
     gene            complement(372..881)
                     /gene="folK"
                     /locus_tag="PK077_08670"
     CDS             complement(372..881)
                     /gene="folK"
                     /locus_tag="PK077_08670"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRN24410.1"
                     /translation="MKRAGIALGSNLGDKNSLMVQARDHLLQMSLSDDPFLQSSLYAT
                     SPQGCPHGADDYLNAVVEFTWPGTVEELLVHCQGIERVLGRIRSGIRCEPRTADVDII
                     YVGDMVVNDPPRLILPHPRAHLRKFVLKPLSDIRPELILPRQHKTVACLLAELDTDEA
                     EPLLVSERW"
     gene            complement(939..1679)
                     /gene="dapB"
                     /locus_tag="PK077_08675"
     CDS             complement(939..1679)
                     /gene="dapB"
                     /locus_tag="PK077_08675"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HRN24411.1"
                     /translation="MISILVTGISGRMGQAIQEAVTQNPDTCVGSTHDQGQELYPALA
                     KCDVAIDFSHHAFTSTLLAEAVANNKPLVIGTTGHTELERQEIVDAAASIPIVFASNY
                     SVGVNALFWLTRKAAQILGGSCDIEVMEMHHRHKIDAPSGTARTLAEILSGAIDRNYE
                     DSVVFGREGLVGPRPAKEIGMHSLRGGDVVGDHTVIFASDGERLELTHKASSRMTFAS
                     GAVRAALWLQGREPGLYTMEDVLGLSQL"
     gene            complement(1692..2573)
                     /gene="dapA"
                     /locus_tag="PK077_08680"
     CDS             complement(1692..2573)
                     /gene="dapA"
                     /locus_tag="PK077_08680"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HRN24412.1"
                     /translation="MKFQGLYTAIVTPFAGNRVDEEAFRMLVEAQIAAGVAGIVAVGT
                     TGESPTLTVKEHLRVIELAVESAAGRIQVIAGTGANSTAEAIHMTQEAEKMGVDGTLQ
                     VCPYYNKPSQNGVYAHFKAIADATRLPIMLYSIPGRCGIEISVETISRLAKDCPHIVC
                     VKEAGGNVDRVNQLMQVVPENFTVLCGDDGLTVPFMACGASGLVSVTSNLVPGIMNSI
                     VKAGLNQDMGEMLSLQKTFYPLMKGLMTLDSNPVPIKAALAMRGDIQPGIRLPLVPLA
                     EEKEAQLSALLQRFNIL"
     gene            complement(2647..3354)
                     /locus_tag="PK077_08685"
     CDS             complement(2647..3354)
                     /locus_tag="PK077_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24413.1"
                     /translation="MKSQVIQKPVVVILGNAGNIGYLAAKAFACQGTVVAMLHEAGEE
                     SCHIVDCLPGSSHLVMSGRLNHSSDLASFAGLVHTLYGRVDYLIICEDASVEVQGLSC
                     SSRVRLSVLLEHAFVSGAMIVNMVSRSGKKGVWVEDIAGLENFLNRRDVRMSQIVIHR
                     PVFRYVSQSRLASSACAAALFLCGNEGRHIHREYIVVNGRKAAWRPGRRPESFLSLFN
                     SCLEFCSICSRMVFQDA"
BASE COUNT          992 a          998 c          800 g          750 t
ORIGIN      
        1 cccccttcca gcttgacagc atccgctcca gccaaaaaca atttctttgc ggattgcacg
       61 gcctgttcca ccgtatcata agaatgtatg ggcatatctc ccaccagcag ggcgttcttc
      121 actccgcgcg cagtagcttc cacatgatga agcatatgat ccagcgtcac atgagtggta
      181 tccggaaagc ccaaaaccac catccccaag gaatccccta ccagcacaat atctacgccg
      241 gcctcatcca aaagacggcc tgtaggatag tcataagcag taaccagagt cacatgccgc
      301 ctgttcttgc aggcgcggat tcgttccgct ttctcaaaag attgattcat ggcgttcaat
      361 aaacatgcaa tttaccagcg ttccgatacc agaagcggct cagcctcatc cgtatccaat
      421 tccgccaata ggcaggcaac cgttttatgc tggcgcggca ggataagctc cggcctaata
      481 tcggaaagag gttttaaaac aaatttcctc aaatgggctc tgggatgggg gagaatcaga
      541 cgaggaggat cattgaccac catgtccccc acatagataa tatccacatc cgccgtgcgg
      601 ggttcacagc gaatcccgga acgaatgcgc cccagaaccc gttctatacc ctggcaatgc
      661 acaagcaatt cctccaccgt acctggccag gtaaattcca ccacggcatt caaataatca
      721 tccgcaccgt gcggacatcc ctgaggactg gtggcataca gggaggattg caaaaaaggg
      781 tcatcagaca ggctcatctg caacaaatga tccctggcct gaaccatcag gctgttttta
      841 tccccaagat tggaccccag tgcaatgcct gctctcttca tcagcaaaaa acatcgtggc
      901 cgttccggaa aagaagaacg gccacgatgc cgaaaaagtt ataattggga caaccccaga
      961 acgtcttcca tggtatacag acccggttcc ctgccttgga gccagagagc cgcacgaact
     1021 gctccggagg caaatgtcat acggctggac gccttatggg taagttccag ccgttcacca
     1081 tcggaagcaa atattacagt atggtccccc accacatccc ctccccgcag ggaatgcatg
     1141 cctatttctt tggcgggtcg aggccctacc aacccttcac ggccaaaaac cacactatct
     1201 tcataattcc tgtcaatggc gccggacagg atttccgcca gtgtccgggc agtaccggaa
     1261 ggagcatcaa ttttgtgacg gtgatgcatc tccattacct ctatatcgca gctgcctccc
     1321 agaatctgtg cagccttgcg cgtcagccag aacagggcgt tcacccctac ggaataattg
     1381 gaagcgaaca caatcggaat ggatgccgca gcgtccacga tctcctggcg ttccagctcc
     1441 gtatgccccg tagtgccaat caccagaggt ttgttattgg caacagcttc cgccagcaga
     1501 gtgctggtga aagcgtgatg ggaaaaatca atggctacgt cgcatttggc gagggctgga
     1561 taaagctcct gtccctgatc atgagtagaa cccacgcaag tatccggatt ctgcgtcacc
     1621 gcctcttgaa tagcctggcc catgcggccc gaaatacctg taacaagaat ggaaatcata
     1681 tcactaaaat gttaaagaat attgaaacgc tgaagaagag cggaaagctg agcttccttc
     1741 tcttccgcca atggaaccag cggaagacgt atgccgggct gtatatctcc cctcatcgcc
     1801 aaagccgcct tgatcggaac aggattggaa tccagagtca ttagcccttt catcaacgga
     1861 taaaatgtct tctggaggga aagcatttcc cccatatcct gattcaggcc agctttgaca
     1921 atggaattca tgatgcccgg aaccagattg gatgttacgg aaaccaatcc gctggcgcca
     1981 caggccataa aaggcaccgt cagaccgtca tcgccacaca gaacagtgaa attttccgga
     2041 accacctgca tcagctggtt cacgcggtcc acattgccgc cagcctcttt cacacaaaca
     2101 atatgcgggc aatccttcgc cagacggctg attgtttcca cactgatttc aatcccgcaa
     2161 cgccccggaa tgctgtacag cataatcggc agacgggtag cgtccgcaat agccttgaaa
     2221 tgggcataaa ctccattctg agaaggttta ttgtaatagg ggcacacctg gagcgttccg
     2281 tctactccca tcttttccgc ttcctgggtc atatgaatag cttctgcggt ggaattagcg
     2341 cccgttcccg caataacctg aatacgacca gccgcgctct ccacggccaa ttctattacc
     2401 ctcaagtgtt cttttacagt caaggtgggg gattctcccg tggtaccgac agccacaatg
     2461 ccggccacgc cggcagctat ctgggcttcc acaagcattc taaaagcctc ttcatcgacc
     2521 cggttgccgg caaacggagt cacaattgct gtataaagac cttgaaactt cataacctta
     2581 cttggaaata tactatctta acagacactt tttgtcacga cagaatacag aaatatggca
     2641 cgcctgtcag gcatcctgaa ataccattcg tgaacaaatg gagcaaaact ccaggcagga
     2701 attaaacagg gacaggaatg attccggccg cctgccgggc cgccaggcag ctttccttcc
     2761 attgaccacg atatattccc ggtgaatatg gcgaccttcg ttcccacata aaaacaacgc
     2821 agccgcacag gcggaagaag ccaatcgaga ctgggaaaca taccgaaata ccggacggtg
     2881 aattacgatc tggctcattc tgacatctct ccggttcagg aaattttcca agcccgcgat
     2941 atcttccacc catactccct tttttccaga acgggaaacc atattcacaa tcatggcccc
     3001 agaaacaaag gcatgttcca gcaaaacgga cagacggaca cgggaagaac aactcagtcc
     3061 ctgcacctcg actgaggcat cttcacaaat aatcaaataa tcaacacgtc cataaagagt
     3121 atgtaccaga ccagcaaaag aagccaaatc ggaagaatgg ttcaggcggc ctgacatgac
     3181 caggtggctg ctacccggga gacaatcaac gatatggcaa gattcttcac cggcctcatg
     3241 aagcatggca accacagttc cctgacaggc aaatgccttg gctgccaaat atcctatatt
     3301 gccggcgttg cccagaatga cgaccacagg tttctgaatg acttgggatt tcatatgccg
     3361 ccattctgac gaaagagtgt aatccgtcaa taccccacac atacttaagt cattaagaaa
     3421 gaaaaaggaa tagttaagaa aaaattatat ggccacgaca tcttacaaga aggaaactaa
     3481 ctaagagaat gtagggggag aagaacggga ccgagcaaga aaagaacagg ccccgcgagt
//
LOCUS       DAOMSU010000227         3524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2654_length_3524_cov_2.667051, whole genome shotgun sequence.
ACCESSION   DAOMSU010000227 DAOMSU010000000
VERSION     DAOMSU010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2654_length_3524_cov_2.667051"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            140..928
                     /locus_tag="PK077_08690"
     CDS             140..928
                     /locus_tag="PK077_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN24414.1"
                     /translation="MKKSLFSLALIASSLLAVQAATVQIDFGRSDATTEGALNMNYDN
                     ASASLGSMPGSVSLAWSTAGGYVSENGYTTTKTAEEEADWKNPFNGSMPFSLGDTFRD
                     GLLTQTADGSGYFTVTFSGLAAGEYALSIFGGFTGKDVFAGQTWTIGNADASNAVWTS
                     FGTDASGNWAEISSVTGDNSGVLTPGNAGTSSATANKGLYSSVENIVVGEDGTLTLTI
                     QGNGSSSYGRTALNYLALTQVPEPATAALSLLGAAALFLRRRRA"
     gene            complement(1141..>3524)
                     /locus_tag="PK077_08695"
     CDS             complement(1141..>3524)
                     /locus_tag="PK077_08695"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRN24415.1"
                     /translation="EGKNGVKNKELFLRFLNGFSQYARLGRVRFRTEGGSPWAITVEE
                     AREKFRDFWTPALEKLIQSWLRMEALHTLRRTRATHGLMLLFEGKYSSLVRNRGRFLF
                     HDVTRMLGGGTITPELKRDLQYRMYCRYDHWMLDEFQDTSQPQWRVIKPFLDDLAESK
                     AGNEGSIFVVGDIKQSVYQWRGGDPELFRSVSSQLQLEQRGMTTSYRSVQPVLDLVND
                     ICDYARTAPGCEPAALEQWGEYPEHRCAPHLEGRPGASQIWQTPKAENVSANDLVCQA
                     AADILERTGALRRGLETAILVSTKNQALVIKGWLTDHGIPAEVCDDVPVGVDSPLGKN
                     LLYFFRWLLMPGDPFVVGLLTHSPLRPLITPGCPESMEWKEWRFLLERDGYAAVMEEL
                     ERRLLRGGTELTDFHRDRLAVWQNEAEQVDEQSVSLDEWIRRMEDLTRREDPAAGIVR
                     IMTIHKSKGLGFDIVILPQIGRDTPFANATHLTHFVKKNRDGGIEGIVIKPPKEIYQE
                     IPQFRKLYGEWRARQQFDGFCKLYVALTRAKRATYVILPYREDKGEETADSMWKVVRS
                     SIRSLNRGTEDILPESGASCLYSRGLEGWYAEFPEIIHKRAEESALEWPTQKPLARER
                     ISPSGLSEEASPLRGEKHAGAGKAAALGSAVHAVFERITRWDDENKPEWALHPATEAE
                     RIVAECMEIPSIRGLFTSPETARIMKEQRIEAIDGNNWISGIIDRLILDDGAAVIVDF
                     KTDHAESPEQLRERHSNQLNAYARIVSRITGIPLERITRIIVSTCLKETVPIQ"
BASE COUNT          699 a          970 c         1000 g          855 t
ORIGIN      
        1 attctcatta aacatgttat atatttctat catcttgatg gaagatgact gatgccataa
       61 aaaataaatg tagtatatga ttaagttttc atgatttcca ttgacaccgg attgttgaac
      121 cggtttactc tcttttgtca tgaagaaatc cttattttct ttagctttga tcgcgagttc
      181 cctgcttgca gtacaggcgg cgacggtaca gattgatttc ggacgcagtg acgccacgac
      241 ggaaggcgct ctcaatatga attatgataa cgcctccgcc tctttgggca gcatgccggg
      301 aagcgtttct ttggcgtgga gcacggccgg aggatatgta tcggaaaacg gttataccac
      361 caccaagacg gctgaggaag aagcagattg gaaaaatcct ttcaacggca gcatgccttt
      421 cagtctgggg gacacctttc gtgacggtct tttaacgcaa acggccgatg gttccggtta
      481 tttcaccgta acgttcagtg gtcttgccgc cggggaatat gccttgtcca tctttggagg
      541 tttcacaggc aaggacgtgt ttgccggtca gacttggacg atcggaaacg ccgatgcttc
      601 caatgccgtg tggacaagct tcggtacaga tgccagcggg aactgggccg aaatttcttc
      661 cgtgacggga gataattccg gcgttctgac tccgggaaat gctggaactt ccagcgctac
      721 cgccaataaa gggctttatt cctcggtgga gaacattgtc gtgggggaag acggaacgct
      781 tacccttacg attcagggga atggttccag ctcttacggc cggacggccc tcaattatct
      841 tgccctgacg caggttccgg aacccgctac ggcagcattg agcctgctgg gcgctgctgc
      901 tctgttcctg cgccgccgca gggcctgatc cggaaagcaa caaagccttg ttatcaacgc
      961 cgctcctaat tcacacggag cggttttttt atttggcgtt tttcctcttc ctgtctgtgc
     1021 ggcagggcat ttctattcca gcctgttttc cgggttcccc tttcccatac tggaaatgag
     1081 caggaatagg agtgaagcgc gggaaaatac agggaaacgg atttgtccgg aaagaaaggc
     1141 ttattggatg ggaacggttt cctttaagca agtggaaaca atgatgcgcg ttatgcgttc
     1201 cagcggtatt ccggtgattc tggacacaat gcgggcataa gcattcaatt ggtttgagtg
     1261 gcgctcgcgc agttgttccg gggattcggc atgatccgtc ttgaagtcca cgatgacggc
     1321 ggctccgtca tcaagaatca gcctgtcaat gatgccggaa atccagttgt tcccgtcaat
     1381 ggcttcaata cgctgttcct tcatgatgcg cgccgtttcc ggagacgtga aaagtccgcg
     1441 gatggatggg atttccatac actccgctac gatgcgttcc gcctccgtag cagggtggag
     1501 agcccactcc ggcttgtttt catcatccca gcgggtaatc cgttcaaaca cggcatggac
     1561 ggcggaacca agcgcggcgg ccttccctgc gcccgcgtgt ttttctcccc ggagcgggga
     1621 ggcttcctcc gacaggccgg agggggatat ccgttctctg gccaacggtt tttgcgtcgg
     1681 ccattccagc gcgctttcct ccgcgcgttt gtgtatgatt tccgggaatt ccgcgtacca
     1741 cccttcaaga ccgcgggagt acaggcatga cgctccggat tccggaagta tgtcttccgt
     1801 tccccggttg agggaccgga tagaggaacg gaccactttc cacatggaat ctgccgtctc
     1861 ttctcccttg tcttcccggt acgggaggat aacgtaggta gcccgcttgg cccgggtaag
     1921 cgccacatac agcttgcaga atccgtcaaa ttgctgccgc gcgcgccatt ccccgtaaag
     1981 tttccggaat tgcggaattt cttgatagat ttctttgggc ggtttgatga cgattccttc
     2041 tattccacca tccctgtttt ttttgacgaa gtgagtcaaa tgcgtggcgt ttgcgaaagg
     2101 agtatccctg ccgatctggg gcaggatgac gatgtcaaat cccagccctt tggatttgtg
     2161 gatggtcatg atgcggacaa ttcccgcggc gggatcttcc cgccgggtca ggtcctccat
     2221 gcgcctgatc cattcatcca gggaaacgct ctgttcgtcc acctgttccg cctcattctg
     2281 ccagacggcc agtcggtcgc ggtggaagtc cgtcagttcc gttcctcccc ggagcagacg
     2341 ccgttccagt tcctccatga cggcggcgta accgtcccgt tccagcagga agcgccattc
     2401 tttccactcc atgctttccg ggcatcccgg cgtgatgaga ggccggagag gggagtgggt
     2461 gagcaggccg acgacaaagg gatctcccgg catcagcagc cagcggaaga agtacagcag
     2521 gtttttcccc agcggagagt ccacgcctac cggaacgtcg tcacacactt ctgcggggat
     2581 gccgtgatcc gtcagccatc ccttgatgac aagagcctgg ttcttcgtac tgaccagtat
     2641 cgctgtttcc aggccgcggc gcagggcccc tgtacgctcc agaatatcag ccgctgcctg
     2701 gcataccagg tcattggcgg agacgttttc cgccttgggc gtctgccaga tttgggaggc
     2761 gccggggcgg ccttccagat ggggggcgca ccggtgctcc ggatattccc cccattgttc
     2821 cagggcggcg ggttcgcacc cgggcgctgt ccgggcatag tcgcaaatat cgttgaccag
     2881 atccagcaca ggctggacgg agcggtagga agtggtcatg ccgcgttgtt ccagttgaag
     2941 ctgtgaagaa acggagcgga acagctccgg gtctccgccg cgccattggt acacgctctg
     3001 cttgatatcc cccaccacga aaatgctgcc ttcatttccg gccttggatt ccgccagatc
     3061 atccaggaaa ggcttgatga cgcgccattg gggctgggac gtatcctgga attcatccag
     3121 catccaatgg tcgtaacggc agtacatgcg gtattgcaga tcccgtttaa gttccggcgt
     3181 aatggtgccc cctcccagca tgcgcgtaac gtcgtggaac aggaatctcc ccctgttgcg
     3241 gaccagggag gagtatttgc cctcaaacag gagcatcagt ccgtgcgtgg cgcgtgtgcg
     3301 gcgcagcgtg tgcagggctt ccatgcgcag ccagctttgg atgagttttt ccagagcagg
     3361 cgtccagaaa tcccggaatt tttcccgcgc ctcttccacg gtaatggccc agggggaacc
     3421 tccctctgtg cggaagcgca cgcggcccag cctggcgtat tgggaaaatc cgttcaggaa
     3481 gcgcaggaac agttctttgt tttttacgcc gtttttccct tccg
//
LOCUS       DAOMSU010000228         3502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2672_length_3502_cov_3.173194, whole genome shotgun sequence.
ACCESSION   DAOMSU010000228 DAOMSU010000000
VERSION     DAOMSU010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2672_length_3502_cov_3.173194"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="PK077_08700"
     CDS             complement(<1..999)
                     /locus_tag="PK077_08700"
                     /inference="COORDINATES: protein motif:HMM:NF012879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRN24416.1"
                     /translation="MKAFTITSILAAACLFPSYSPAGAAAENKIQAASMQTAAGIPKQ
                     DPQLIKGKLANGLTYFIRPNAEPKGRFSIRLRVNTGSLNETDDIQGVSHFLEHMVFNG
                     STHFKRGEMIPAMQKEGLGLGGDANAYTAFDETVYMMDVPSMKESTVDLAFTIMRDFA
                     DGALLEESAIDAERGIITSEYKVRDSAGYRVMKEVFSIMLDGTRIPDRYPIGTLEVIR
                     TAPREKFINYYRTHYVPSQMQLVIAGDITPEQGKAWVEKYFGSMKKDNYSFQTDRGTL
                     KTATETTAHWITNKEATSTEASINIARPYVNKPDTVANRNKDIPLNVAYAMLNRRLE"
     gene            complement(1107..2312)
                     /gene="lpxK"
                     /locus_tag="PK077_08705"
     CDS             complement(1107..2312)
                     /gene="lpxK"
                     /locus_tag="PK077_08705"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HRN24417.1"
                     /translation="MKSRSEEFEEWGTEVIFGRAKGFRAAMMRMILRGASWLFRLVVL
                     ARLYLFHSSIARQARLGMLVVSVGNITVGGTGKTPVVELLARTLTRRGRKVAILTRGY
                     KSSELDKPQEWRDKDGRLPENLPKIASDGKTRYLGPLHSGDEPFMLAKNLDGVAVLVD
                     KNRIKSGIFAIEHLGCDTLLLDDGMQYLKLAHELDIVLVDCGAPFGTGAMLPRGTLRE
                     PRSSLARASYIILTKCGGKPQDELISAIRKYNPVADIIVSDHGPRYLENVFTGERLPL
                     EALRGKWVACLSGIARPESFENSLRSLGAHVEICRRFPDHHWFEQTELQEFYDRCADR
                     AMDMIVTTEKDAVRLEKPEENPEVPIYFLRIEVEIYQGREAWERCVDRICGISEPRPR
                     DEWMPSSSL"
     gene            complement(2344..3063)
                     /locus_tag="PK077_08710"
     CDS             complement(2344..3063)
                     /locus_tag="PK077_08710"
                     /inference="COORDINATES: protein motif:HMM:NF014630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HRN24418.1"
                     /translation="MLGWQAMNHADLDQLLILQEKDVRISKLRKELASLPEQRTRLLK
                     QMEAIKQKALAAKQEVAGIEKSIRDVEAAVETKRTYIGKMKTLQSNTRKNEEYQMCIQ
                     EVEKTEAAIDSLETSELELMERLETAKTDMEQKIRRARDAQRELEETLARFDRTAETD
                     KELLNRLNAERADLAAAVPEDSLGEYERMTRSKGVPVIVPMDEKGHCGGCHMVITDNA
                     RMKVLGGHETVYCDSCHRILH"
     assembly_gap    3211..3249
                     /estimated_length=39
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT          625 a          975 c         1006 g          857 t
ORIGIN      
        1 ttccagacgg cggttcagca tggcgtaagc cacattcaag gggatatctt tgttgcggtt
       61 ggcgacggta tccggcttgt tcacgtaggg gcgggcgatg ttgatgctgg cttccgtgct
      121 ggtggcctct ttgttggtaa tccagtgggc ggtggtttcc gtagccgttt taagcgtgcc
      181 ccggtccgtc tggaaggagt agttgtcctt cttcatggag ccaaaatact tttccaccca
      241 ggcttttccc tgttccggtg tgatgtcccc ggcgatgacg agctgcattt ggctgggtac
      301 gtagtgagtc cgataatagt tgataaattt ttcccgcggc gcggtgcgga ttacttccag
      361 cgtgccgatg ggatagcggt cgggaatgcg ggttccgtcc agcatgatgg agaagacttc
      421 cttcatgacc cggtaaccgg cggagtcacg caccttgtat tcgctggtga tgatgccgcg
      481 ttccgcgtca atggcgcttt cttccagcag ggctccgtcc gcaaaatccc gcatgatggt
      541 gaaagccagg tccacggtgg attccttcat gctgggcacg tccatcatgt acacggtttc
      601 gtcaaaggcg gtgtacgcat ttgcgtcccc tcccaggccc aggccttctt tctgcatggc
      661 gggaatcatt tccccgcgct tgaaatgggt gcttccgttg aaaaccatgt gttccaggaa
      721 gtgggaaacg ccctggatgt catccgtttc attcagagaa cccgtgttga cgcgcaggcg
      781 gatgctgaac cggcccttgg gttccgcatt cgggcggatg aagtaggtca ggccgttggc
      841 gagtttcccc ttgataagct gggggtcctg cttgggaatg cctgccgccg tttgcattga
      901 ggcagcctga atcttgtttt ctgctgcggc tccggccggc gaataggacg gaaacaggca
      961 cgcagcagcc agaatggagg tgatggtaaa tgccttcatg ggaaaatact gcctttttgt
     1021 ttcacacgat tttccgggca agtcaagcgg ccttctctgc ataaaagagg tgaatttccg
     1081 gaaaacctca ggaaacgtga accatgtcaa agggacgaag aaggcatcca ttcatcccgt
     1141 gggcgcggtt ctgaaattcc gcagatgcgg tctacacaac gttcccatgc ctcccggccc
     1201 tggtaaattt ccacctcaat gcgcaggaaa tagataggaa cctccgggtt ttcctccggt
     1261 ttttccagcc gcacggcatc cttctccgta gttacgatca tgtccatggc ccggtctgcg
     1321 cagcggtcgt aaaattcctg caattccgtt tgttcaaacc agtggtggtc cggaaacctg
     1381 cggcagattt ccacgtgggc gcccagggag cgcagggagt tttcaaaact ttccggacgt
     1441 gcgatgccgc tgaggcacgc cacccatttc ccccggaggg cctccagagg aaggcgttcc
     1501 cccgtgaaca cattttccag ataccggggg ccgtggtcgc tcacgatgat gtccgccacg
     1561 ggattgtact tcctgatggc ggagatcagc tcatcctgtg gctttccgcc gcatttggtc
     1621 agaatgatgt aactggcgcg cgccagactg ctcctgggtt cccgcagggt gccgcgcggg
     1681 agcatggctc cggtgccaaa gggcgcgccg caatccacca gcacgatatc cagttcatgc
     1741 gccagtttca aatattgcat gccgtcgtcc agcaacaggg tgtcgcaccc caggtgctca
     1801 atggcgaaga tgccggactt gatgcggttt ttgtccacca gcaccgccac gccgtccaga
     1861 tttttggcga gcatgaaagg ttcatctccg gaatgcaggg ggcccaggta gcgcgttttc
     1921 ccgtcgctgg cgattttggg aagattttcc ggcagcctgc cgtccttgtc cctccattcc
     1981 tgaggtttat ccagctctga gctcttgtag ccgcgtgtca ggatggctac tttgcgcccg
     2041 cgccgggtaa gggtgcgggc cagcagttcc accaccgggg tttttccggt tcctcccacg
     2101 gtgatgttgc ccacgctgac caccagcatt cccagcctgg cctgcctggc gatgctggaa
     2161 tgaaacagat acagccgtgc cagaaccacc agcctgaaca gccaggaggc gccgcgcagg
     2221 atcatgcgca tcatggcggc gcggaatccc ttggcacggc cgaagatgac ttccgtcccc
     2281 cactcttcaa attcttccga tctggatttc atggcaagcc cggcggcgtt cctggaaact
     2341 ccttcagtgg aggatgcggt ggcagctgtc gcagtaaacg gtctcgtggc cgcccagcac
     2401 tttcatgcgg gcgttgtccg tgataaccat gtggcagccg ccgcaatggc ctttttcatc
     2461 cattggcaca atgaccggaa cccccttgct tctggtcatc cgttcatatt cccccaggga
     2521 atcttccgga acggcggcgg ccagatccgc gcgttccgca ttcaaacggt tcaggagctc
     2581 cttgtccgtc tctgccgtcc ggtcaaagcg cgcgagcgtt tcctccagtt cacgctgggc
     2641 gtcccgtgca cggcggattt tctgctccat gtccgttttt gctgtttcca ggcgctccat
     2701 caattccagc tctgaggttt ccagagagtc aatagcggct tccgtttttt ccacctcctg
     2761 gatgcacatc tggtattcct cgtttttccg ggtgttggac tgaagggttt tcattttgcc
     2821 gatgtaggtg cgtttggttt caacggcggc ctccacgtcc cgtatgcttt tttcaatgcc
     2881 tgccacttcc tgtttggcgg ccagggcctt ctgcttgatg gcctccatct gtttcagcag
     2941 gcgcgttctc tgttccggca gggaagccag ctccttccgg agtttggaaa tccgcacatc
     3001 cttttcctga aggatcagca actggtctaa atcggcatga ttcatcgcct gccaccctag
     3061 catgccctgt cggggggggg aaagattttt tcttggctcc ggggcggcgg ttctgccgct
     3121 tgttgacggt gcggggaagt ctgagaaata tttttctgtt ctctgctaag tatttcaagc
     3181 agggctttct gttgtgtcgg cgtgcttccc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnng ccgcgacagc cggtcttttt tctcctggtt ccgccaggaa aggaaggcgc
     3301 atatggcaag ggcgcagacg cagaagattg ccaggaccat gtatgaatgg cggttggagg
     3361 gcatggagga agggaactgc cctgcattat agaaaaaact gtcttccgct caatgtaaat
     3421 aggcaggcag gggaaaacgg cgtttgcggg gggaagagtg ttcgtccctt gcctgttctc
     3481 cctgagcgga gaaagccttc ag
//
LOCUS       DAOMSU010000229         3480 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2697_length_3480_cov_2.551533, whole genome shotgun sequence.
ACCESSION   DAOMSU010000229 DAOMSU010000000
VERSION     DAOMSU010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2697_length_3480_cov_2.551533"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="PK077_08715"
     CDS             complement(<1..1383)
                     /locus_tag="PK077_08715"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HRN24419.1"
                     /translation="MNKLTTFFLGTVLSSSMLPGWTAAPPKPYGAIPTPAQIKWQRME
                     FYGFIHFGLNTFTGREWGYGDEDPKIFNPTDFNASDIVSTFKKGGMKGMIYTAKHHDG
                     FCAWPTKSTDHNITKSPWKNGKGDVVREFALACKKHGIKFGTYLSPWDRNNADYGKDG
                     YLDVYYKQIRELLTNYGPVFEIWFDGANGGDGYYGGAREKRNIGDAEKYYNFEKIVEM
                     IRKIQPSCIIWGAGHYGDARWGGSEKGHVNYPHWSTVGLNGGGGGTGMRGGERWVPAE
                     GDTTINHAGWFWHQGQASRVKSPEELMQVWFDSVGRGANLILNVAADKTGRLDPADVK
                     SLLEFKELRDKLYARDYALGATVTASQTRGNDKKFSPSNMTDGNIETYWAVEDDNLTP
                     TAFITLPKPATFDVIRLREQTRLGQRVDSFNIDAFINGKWVCIDNEGKTIGNQVMRRL
                     NRPITTQKLRL"
     gene            1571..2248
                     /locus_tag="PK077_08720"
     CDS             1571..2248
                     /locus_tag="PK077_08720"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRN24420.1"
                     /translation="MSYFRFLLLALVFLTGSALISESAPVYMARRDSKFALVPLCDGF
                     DFPVGKPDGNGYYRSRGLRLKSPRHMGEDWNGNKGGNSDLGDPVYSVGHGVVTYAADA
                     RGAWGKVVIVRHAFREPKSGKVLCCQTLYSHLNDINVVLGQLVLRGTQVGTIGTNRGM
                     YPAHLHVELHYNPDVNCGHQGIPKTERNYGRLTDFITRFRRLPLEKRMVRVPIGGFLP
                     YKGTEGL"
     gene            2265..2840
                     /gene="nadD"
                     /locus_tag="PK077_08725"
     CDS             2265..2840
                     /gene="nadD"
                     /locus_tag="PK077_08725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HRN24421.1"
                     /translation="MKLCIFGGSFDPVHEGHVCVAGHARKYCGMDRVLFMPCSLSPLK
                     EQAPSVTDDQRCRMIELALQGLDWAVLDRTDLELPPPSWSWRVAERTAERYPGAELFW
                     LMGKDQWDSLEQWGRWEYLSSLVTFIVYRRGGVPSPRKGVRALFIEGDEPASSTRIRH
                     DLRSGVCPVPHLNRKVEELIRREGLYGAARV"
     gene            complement(2860..>3480)
                     /locus_tag="PK077_08730"
     CDS             complement(2860..>3480)
                     /locus_tag="PK077_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRN24422.1"
                     /translation="LGQVFHADAPDTHRIPADKWPAALNTRLPRTIRITHAEYVPPDF
                     HARFSATGKTYRYCISRAPILNPFDAGLAWHRPLAWSVDILEQAVHLFRGTHDFTAFA
                     ALRGNEPRPIPEDYFRRTIMQAQVEQTGEHVFITFTGTGFLYKMVRLMTGAAHEAARG
                     KITLDELARLINAPLPDDKSPFCAPPDGLTLMQVHYPEETFGDKKK"
BASE COUNT          637 a          876 c         1083 g          884 t
ORIGIN      
        1 caggcgcagt ttctgggtag tgatggggcg gttcaggcgg cgcatgacct ggttgccgat
       61 ggttttccct tcattgtcga tacaaaccca tttgccattg atgaaagcgt caatattgaa
      121 ggagtccacg cgttgtccca ggcgtgtttg ttcgcgcagc cggatgacgt cgaaagtggc
      181 gggtttgggc agcgtaatga aggcggtggg cgtcaggttg tcgtcctcca cggcccagta
      241 ggtttcaatg ttgccgtccg tcatgttgga gggagagaat tttttgtcgt tgccgcgcgt
      301 ctggctggct gtgacggttg ctcccaacgc gtaatcccta gcatatagct tatcgcgcag
      361 ttccttgaat tccagcaggg atttgacatc cgcaggatcc agtctgcctg ttttgtccgc
      421 cgccacgttc agaatgaggt tagctccgcg gcctacggaa tcaaaccata cctgcataag
      481 ttcttcaggg gatttgacgc gggatgcctg tccctggtgc cagaaccagc cggcatggtt
      541 gatggtggtg tctccttccg ccggcaccca gcgttctccg ccgcgcatgc cggtgcctcc
      601 gcctccgccg ttcagcccta cggtgctcca atggggataa ttgacgtggc ctttttcgga
      661 gcctccccag cgggcgtctc cgtagtggcc cgctccccag atgatgcagc tgggctggat
      721 tttgcggatc atttccacga ttttttcaaa gttatagtat ttttccgcgt caccgatatt
      781 gcgcttttcc ctggctcctc cgtaatagcc gtctcctccg ttggctccgt caaaccagat
      841 ttcaaagaca ggcccgtaat tggtcagcag ttcccggatt tgtttatagt aaacgtccag
      901 gtagccatcc ttgccgtaat ccgcgttgtt gcggtcccag gggctgaggt aggtgccgaa
      961 tttgatgccg tgctttttgc aggcaagggc gaattcccgg actacatctc cttttccgtt
     1021 tttccaaggg ctcttggtga tgttgtggtc cgtggatttg gtgggccagg cgcagaagcc
     1081 gtcatgatgt ttggccgtat aaatcattcc tttcattccg ccttttttaa aggtggagac
     1141 gatatcggag gcattaaaat ccgtggggtt gaagattttc gggtcttcat cgccgtaacc
     1201 ccattcccgt cccgtgaacg tattcaggcc aaagtggatg aagccgtaga attccatgcg
     1261 ctgccatttg atttgggcag gtgttggaat cgcgccgtat ggtttgggcg gtgctgcggt
     1321 ccagccgggc agcatggagg aagagagaac tgttccgagg aagaatgttg ttagcttatt
     1381 catgcagaaa aaagtgtgtt gaaaaatacg gagtcgctcc tcccttcttt catacggggg
     1441 ctttgctgtc aagttgaaag gaagcgggaa acaggggaca gcgtgttagt gaattctaac
     1501 ttcgacatgc ggggaattcc gcttgatacg gagtagggag ccgtttagca tacaaaggac
     1561 tttcgttccc atgtcctatt tccgtttttt actgctggcg ctggtttttc tgaccggttc
     1621 cgcgttgatc agcgagagcg ctcccgttta catggcccgg cgggacagca agtttgcgct
     1681 tgttcctctt tgtgacggtt ttgattttcc ggtaggaaag cctgacggca acggctatta
     1741 ccggtcccgc ggtctgcgtc tgaaaagccc ccgtcacatg ggtgaggatt ggaacggcaa
     1801 taaaggaggc aattccgatt tgggcgatcc cgtttattcc gtaggccacg gggtggtaac
     1861 ttatgcggcg gacgcccggg gcgcgtgggg aaaggtggtg attgtgcgcc atgctttccg
     1921 cgaacccaag tccggaaagg ttctttgctg ccagacgctt tattcccatt tgaacgatat
     1981 taacgttgtg ctgggacagt tggtgctgcg cggcacccag gtaggcacta tcggcacgaa
     2041 ccgcggcatg tatccggccc atcttcatgt tgagctgcat tacaacccgg atgtgaattg
     2101 cggccatcag gggattccta agacggagcg caattatggc cgcctgacgg attttattac
     2161 ccgcttccgc cgcctgcctt tggagaaaag gatggtgcgc gtccccatcg gcgggtttct
     2221 gccttacaag ggaacggaag gactttgact tctgctggat gacggtgaag ctgtgtattt
     2281 tcggcggttc gtttgatccc gtgcatgagg ggcacgtttg cgtggccggg catgcacgga
     2341 aatattgcgg aatggaccgc gtgctgttta tgccgtgttc cctgtctccg ctgaaggaac
     2401 aggccccctc cgtgacggat gaccagcgtt gccggatgat tgagctggct cttcaagggc
     2461 tggattgggc tgtgcttgac cggacggatt tggaacttcc gcctccttca tggtcgtgga
     2521 gggtggctga gcggacggcg gagcgttatc ccggtgcgga attgttctgg ctgatgggca
     2581 aggatcagtg ggattctctg gaacagtggg gacgctggga atatttgtcc agcctggtga
     2641 cgttcattgt ttaccgccgc ggcggggttc catcacccag gaaaggggtg cgcgcccttt
     2701 tcatagaagg ggatgaaccc gcttcttcca cccgcatacg gcatgatttg cgttcgggcg
     2761 tatgtccggt tccccatctg aaccggaagg tggaggagct gatcaggagg gaagggctgt
     2821 acggcgcggc acgcgtctag aaggacggct ggttccagtt tatttctttt tgtctccgaa
     2881 cgtttcttcc gggtagtgga cctgcatcag cgtgagtccg tccggagggg cgcaaaacgg
     2941 gcttttgtca tccgggagcg gcgcgttgat gaggcgcgcc agttcgtcca gcgttatttt
     3001 tccccgtgcg gcttcatggg cggctcccgt catgaggcgc accattttgt agaggaagcc
     3061 cgttccggta aaggtgatga agacatgttc ccccgtctgt tccacttgcg cctgcatgat
     3121 ggtccggcgg aagtaatcct ccggaatagg gcgcggttca ttgccgcgca gggcggcaaa
     3181 ggcagtgaag tcatgcgttc cccggaacag gtgtacagcc tgttccagaa tatccacgct
     3241 ccatgccagc gggcggtgcc aggccagccc ggcgtcaaaa gggttgagga tgggtgcgcg
     3301 tgaaatgcag taacggtagg tttttcccgt ggcgctgaac cgggcatgga aatcgggcgg
     3361 aacgtattcc gcatgggtaa tccggatggt tcggggtagg cgcgtgttga gcgcggcagg
     3421 ccatttgtct gcaggaatgc ggtgggtatc cggagcgtct gcatgaaaaa cctggcccag
//
LOCUS       DAOMSU010000230         3470 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2707_length_3470_cov_2.582430, whole genome shotgun sequence.
ACCESSION   DAOMSU010000230 DAOMSU010000000
VERSION     DAOMSU010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2707_length_3470_cov_2.582430"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="PK077_08735"
     CDS             complement(<1..327)
                     /locus_tag="PK077_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024671.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4065 domain-containing protein"
                     /protein_id="HRN24423.1"
                     /translation="MANSTTTQKLCNWFIQQANAKELILTPVKLNHLVILADWWHLHR
                     TGAFLINERVEAWPSGPVLPSIYHEYKDQPPYSSIEHPSRRQPPLEEETDIIPFLEHI
                     WNVYGKY"
     gene            complement(636..1451)
                     /gene="thiM"
                     /locus_tag="PK077_08740"
     CDS             complement(636..1451)
                     /gene="thiM"
                     /locus_tag="PK077_08740"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HRN24424.1"
                     /translation="MLSSTDLVQAVTADLEKIRETAPLVLSLTNSVVQPLTANLLLAI
                     GAVPAMLNDAEEAVDMLRSGTGALLVNLGTVTREQGAAMQTAVREANRLNIPWVLDPV
                     AVGALSLRTRLAGQLKEQSPRIIRGNASEIMALAGYSSVTKGPESTSSSADALHAARE
                     LALHTGAAVLVTGRTDYSTDGRQVTATENGHAMMSRVTGVGCSMGALSAACAAVSPTP
                     LQAAVSTAVLMGIAGEMAFEQSPSPGSFAVSLLDSLYALSPEDVVRRARFLSL"
     gene            complement(1499..2113)
                     /gene="thiE"
                     /locus_tag="PK077_08745"
     CDS             complement(1499..2113)
                     /gene="thiE"
                     /locus_tag="PK077_08745"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HRN24425.1"
                     /translation="MKKFNLHLYLVTDEASKCRLSLLETVRKAADGGVTIVQYRSTNP
                     DAGTCYREALPIRDFLASRGIPFIVNNRIDLALALEADGVHIGQRDLPVPSVRAMIGP
                     DKILGLSVSNKEQLRAVDASLVDYLGMGPVFPTISKLNAPPVLGVEGFAALASQSPLP
                     VVAIGGLDAERARQVRATGTASGIAVVSAICGAENPEAAARALA"
     gene            complement(2132..3061)
                     /gene="cysK"
                     /locus_tag="PK077_08750"
     CDS             complement(2132..3061)
                     /gene="cysK"
                     /locus_tag="PK077_08750"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HRN24426.1"
                     /translation="MRIYKNITELIGGTPLLELTNYERRNGLNATILAKLEYLNPAGS
                     VKDRIAQAMIDAAEAAGKLKPDSVIIEPTSGNTGIGLAAVAASRGYRIILTMPETMSV
                     ERRNLLKAYGAELVLTDGALGMKGAIAKAEELAAGLPNSFIPGQFANPANPEAHFRTT
                     GPEIWNDTEGKADIFVAGVGTGGTITGVGKYLKSRNPGIRVVAVEPFSSPVLTQGTAG
                     PHKIQGIGAGFVPGTLDTSVYDEVIAVTNEDAFSTGKELARTDGVLTGISSGAALWAA
                     TQVARRPENEGKTIVVLLPDTGDRYLSTPLFTD"
     gene            complement(3098..>3470)
                     /locus_tag="PK077_08755"
     CDS             complement(3098..>3470)
                     /locus_tag="PK077_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HRN24427.1"
                     /translation="ADYLASHPQVERVNHPSLPDHPDHDLYKRYYPNGGGSIFTFEIK
                     GGAEKARKFCESLELFSLLANVADVKSLVIHPASTTHSQMTEEELKAGGITPSTVRLS
                     IGTEHIDDILEDLEQGFRAIL"
BASE COUNT          647 a          972 c         1123 g          728 t
ORIGIN      
        1 atatttgcca tagacgttcc agatatgctc cagaaagggg ataatgtccg tttcctcctc
       61 caaagggggc tggcggcggc tgggatgctc tatggaagag tagggcggct gatccttgta
      121 ttcatgataa atggaaggaa gtaccggacc ggaaggccag gcttccaccc gttcattgat
      181 caggaaagcg ccagtccggt gcagatgcca ccagtccgcc agaatgacca gatggttcag
      241 tttgacgggc gtcaggatga gctccttggc gttcgcctgc tgaatgaacc agttgcagag
      301 tttttgcgtg gtagtggagt ttgccatgct gaaatgggtt gcctccatta taaaaggccg
      361 caggtatttc tcaacactaa atccgcttgc ggttccggcg ctctggcggt cggcataggc
      421 aaatggctgt gccgggattc gtcaggtccc cggcatgccg cttcctgctc cgcaggcgga
      481 atgcctgaaa atatggcgca ggttgttcta gcggggaggg tgccggaggt tcccctctcc
      541 cgggcagctc ctggtgatgt gcttcccctt ccggagattt ccgggtcagg ccgtgtgcat
      601 ggcagcggag ttgctttccg gggaagagaa gactgtcaaa gggaaagaaa gcgcgctctg
      661 cggacaacgt cttccggaga aagggcgtaa aggctgtcca gcaatgatac ggcaaaggaa
      721 ccgggggagg ggctttgttc aaaggccatt tctccggcaa tgcccatgag tacggctgtg
      781 gaaacggccg cctgcagggg ggtgggggag acggcggcgc aggcggcgga cagggctccc
      841 atggaacagc ccacgcccgt aacccgggac atcatggcgt ggccgttttc cgtggcggtt
      901 acctggcggc cgtcagtgga ataatccgta cgccccgtaa cgagcacggc cgcccccgtg
      961 tgcagggcca gttccctggc cgcatgcagg gcgtctgcgc tggagctggt gctttccggc
     1021 cctttcgtga cggaggaata gccggccagg gccatgattt cagaagcgtt tccgcggatg
     1081 atgcgggggg attgttcctt caattgcccc gccagccgcg tgcgcaggga aagagcccct
     1141 acggccacag gatccagcac ccaggggata ttcagcctat tggcttcccg caccgccgtt
     1201 tgcatggcgg ctccctgttc acgcgtcacg gtgcccaggt tgaccagcag ggcgcctgtt
     1261 ccgctgcgga gcatgtccac cgcttcttcc gcgtcgttga gcatggcggg gacggcgcct
     1321 atggccagca ggagattggc cgtcaggggc tggacgacgg aattggtcag ggagagaacc
     1381 agcggggccg tttcccggat tttttccaaa tcggcggtaa cggcttgaac aaggtctgtc
     1441 gatgaaagca tggcgtaaaa aagtgcttga ttgtccggtg tttcctgcgg ttcgtccgtc
     1501 aggcaagagc ccgtgcggcg gcttccggat tctccgctcc gcaaatggcg gagacaacgg
     1561 caattccgga ggcggttccc gtggcgcgca cttgacgggc ccgttccgcg tccagtccgc
     1621 cgatggcgac aacgggcagg ggagattggg aagccagagc ggcgaagcct tccacgccca
     1681 gcacgggagg cgcgttcagc ttggaaatgg tggggaagac ggggcccatg cccaggtagt
     1741 ccaccaggga ggcatccacg gcgcggagct gttccttatt ggagacggac agtcccagga
     1801 ttttatccgg gccgatcatg gctctgacgg atggaaccgg aagatccctc tggccgatgt
     1861 ggacgccgtc tgcttccagc gccagagcca ggtcaatgcg gttgttgacg atgaagggaa
     1921 taccgcggga agccaggaaa tcccgtatgg gcaaagcttc ccggtaacag gttcccgcat
     1981 ccggattggt ggagcggtac tgcacgatgg taactcctcc gtccgccgct ttccgtacgg
     2041 tttccagaag gctgaggcgg catttggatg cttcatccgt aaccaggtag agatggagat
     2101 tgaatttttt catggcaggg aatggaagga atcagtccgt aaacagaggg gtggaaagat
     2161 agcggtcccc cgtgtccggc agcaggacga cgatggtctt tccttcattc tccgggcgcc
     2221 gcgccacctg tgtggcggcc cagagagctg ctccggagga aatacccgtc agcacgccgt
     2281 ccgtgcgcgc cagttccttc cctgtggaga aagcgtcttc attggtaacg gcgatgactt
     2341 catcgtacac ggaggtgtcc agcgttcccg gcacgaagcc cgcgccaatg ccctgaatct
     2401 tgtgggggcc ggccgtgccc tgggtcagca cgggggagga gaacggctcc acagcaacga
     2461 cccggatgcc cggattacgg gatttaaggt acttgcccac gcctgtgatg gtgcctccgg
     2521 tacccacgcc cgccacaaaa atatccgctt tgccttctgt atcattccag atttccgggc
     2581 ctgtggtgcg gaaatgcgct tccgggttgg ccgggttggc gaactggccg ggaataaagc
     2641 tgttgggcag tcccgcggcc agttcctccg ccttggcgat ggctcccttc atgcccagcg
     2701 cgccgtccgt cagcaccagt tccgcgccgt aggctttcag gaggttgcgg cgttccacgc
     2761 tcatcgtctc cggcatggtc aatataatcc ggtacccgcg tgaagcggcc accgccgcca
     2821 ggccgatgcc cgtgtttccg ctggtgggct caatgatgac ggaatccggc ttcagcttcc
     2881 cggcggcttc cgccgcatca atcatggcct gggcaatgcg gtctttcacg ctgccggcgg
     2941 ggttcaggta ttccagcttg gccaggatcg tggcgttcag gccattcctg cgttcgtaat
     3001 tggtcagttc cagcaacggg gtgccgccga tcagttccgt aatgtttttg taaattctca
     3061 tggcttgtag gaaaagagcg ttgttgccgt taaatcgtta gagaatggcg cggaagcctt
     3121 gttccaaatc ttccagaata tcgtcgatat gttccgtccc gatggaaagc cgcacggtgg
     3181 atggcgtaat gcctcccgcc ttcagttcct cctccgtcat ctgggaatgg gtggtggagg
     3241 ccggatgaat caccagggac ttgacgtccg ccacgttcgc gagcagggag aatagttcca
     3301 ggctttcgca gaacttgcga gctttttccg cgccaccctt gatttcaaag gtgaagatgg
     3361 agcctccccc gttcggatag tatctcttgt aaaggtcgtg gtccggatgg tccggcaggg
     3421 aggggtggtt caccctctcc acctggggat gggaggccag atagtccgct
//
LOCUS       DAOMSU010000231         3386 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2809_length_3386_cov_2.599219, whole genome shotgun sequence.
ACCESSION   DAOMSU010000231 DAOMSU010000000
VERSION     DAOMSU010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2809_length_3386_cov_2.599219"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1523)
                     /locus_tag="PK077_08760"
     CDS             complement(<1..>1523)
                     /locus_tag="PK077_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRN24428.1"
                     /translation="HVTRLDDIARIELGAETYTGSSRNNGEDSVNMAVYRLDDANALE
                     AMNGVKDTLEKLEKRFPEGVSYVVSYDPTQYISATMAEIVETLVIALILVVGITYLFL
                     QDWRATLIPALAIPVSLIGTFAILLPLGFSINVLTMFGLILVIGSLVDDGIIVVENTM
                     RILETEDLSPEEATKKSMHQITGAIIATTLVTVAIYVPIAFFGGMVGNIYMQFSVTMC
                     VALCLSAINSLTLSPALCVLLLKRKQKKQSRFSLFRPFNASLEWARKSYIKCAGIMVR
                     RAWLTLILLAAVLVGNWKLFETVPKSFLPPEDKGTVFCDIQLAPGATLGRTEQAMRSA
                     EQKLMSIPGVRQVSSTSGFSFMGGNGENLGMCIAQLDPWDKRKTPELSLDSIMQKASV
                     LCDEIPAAKATVFSPPAIMGLGLTGGVSFMLQASGEETPKDLERVTNDLLDKINKLPG
                     TMYARSAYEANTPQLFLNIDREKAQSMHVPVSRIFTTLQSKLASMYINDFNLIGYTF"
     assembly_gap    1524..1534
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1535..2247)
                     /locus_tag="PK077_08765"
     CDS             complement(<1535..2247)
                     /locus_tag="PK077_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRN24429.1"
                     /translation="MIADLFIKRPKFAIVIAILMMLAGGICLNQLPIAEYPEIAPTSI
                     NVQATYTGASAQVVMETLASPIEEELNGLENLLYFSSKSDNTGGYSLSLTFKSGTNSD
                     INMVNVQNALKRVEYKLPKEVTDQGIKIKKRSSDILGFFAFRSTSMSSLELNNFVKTR
                     VKDEVARVPGISAVNLMPEKNYSMRIWLDALRMSALNITPDDVSNAIKAQNVQAAAGS
                     IGSEGENNFIQYKVNVTGRL"
     gene            complement(2255..>3386)
                     /locus_tag="PK077_08770"
     CDS             complement(2255..>3386)
                     /locus_tag="PK077_08770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRN24430.1"
                     /translation="KHTHHRKAPLRLLAVSGLLFSLFVPGYSQGTPGGAAAKPSTVLV
                     QKAAAIDSAVNKKYIGQVEAIDRVTVQPRVSGNIVATRFQEGKVVKKGDLLFEIEDTR
                     YRAAVEEAVAKKAQLEAKLLYAKNSFERYNRLLASKSVSMDTVENAKSTMHALEAEIQ
                     SANAAITVAKDNLNYTRLTAPITGRTGRVTFSTGNYITPTSGSLVTITGIDEVYVKFP
                     ISERDFLSLFGTQENMKKDALVSVNLANGKAYDQPGRIFMTDNTVQTTTDTLNVWAKF
                     PNPEDVLTPGGVVTVNLSKKNVDRFPAANISSVMHDAYKSYVYIVNDQGVVERRDVTL
                     GNTVNNEQCFSSGVKEGEVVIIDGMHKVRPGAKVNPVYSVQN"
BASE COUNT          657 a          896 c         1030 g          792 t
ORIGIN      
        1 tgaacgtgta gccgatcagg ttgaaatcat tgatgtacat ggaggccagc ttgctttgaa
       61 gcgtcgtgaa gatgcggctg acgggcacgt gcatgctctg agccttttca cggtcgatgt
      121 tcaggaaaag ctggggggtg ttcgcctcat acgcgctgcg ggcgtacatg gttcccggca
      181 gtttgttgat tttgtccagc aggtcgttgg tcactcgttc caggtctttg ggagtttcct
      241 ccccgctggc ctggagcatg aaggagacgc cacccgtcag gcccagcccc atgatggcgg
      301 gcgggctgaa cacggtggcc ttggccgccg gaatttcatc acacagaacg gaagctttct
      361 gcatgatgga atccagggag agttccggcg tcttgcgttt gtcccagggg tcaagctggg
      421 cgatgcacat gcccaggttt tctccgttgc cgcccatgaa gctgaatccg gaagtggagg
      481 aaacctggcg cacgccgggg atgctcatca gcttctgttc cgcactgcgc atggcctgtt
      541 ccgtacggcc cagcgtggcg cccggggcca gctggatatc acagaaaacg gtgcccttgt
      601 cttccggcgg gaggaaggat ttgggtacgg tctcaaacag tttccagttg ccgaccagga
      661 cagcggccag cagaatcagc gtaagccacg cgcggcgcac catgatgccg gcgcatttga
      721 tatagctttt gcgcgcccat tccagagagg cgttgaaggg gcggaagagg ctgaacctgc
      781 tctgtttctt ttgcttcctt ttcagcagca gaacgcacag cgcggggctg agcgtcaggg
      841 aattgattgc ggaaaggcag agggccacgc acatggttac ggagaattgc atgtaaatgt
      901 tccccaccat gccgccgaag aaggcgatgg gcacgtaaat ggctaccgtc accagcgtgg
      961 tggcgatgat ggcgcctgta atctggtgca tgctcttctt ggtggcttct tctggcgaga
     1021 gatcctccgt ctccagaatg cgcatcgtat tttccaccac gatgatgccg tcgtccacca
     1081 gggacccgat taccagaata aggccgaaca tggtcagcac attgatggaa aagcccaggg
     1141 gcagcaggat ggcgaaggtt cctatcaggg agacgggaat ggcgagcgcc ggaatgagcg
     1201 tggcgcgcca gtcctgcagg aacaggtacg tgatgcccac caccagaatc agggcgatga
     1261 caagcgtttc cacgatttcc gccatggtgg cggaaatgta ttgcgtgggg tcgtagctca
     1321 cgacgtagct cacaccttcc ggaaaacgtt tttccagctt ctccagcgtg tctttcacgc
     1381 cgttcatggc ttccagggca ttggcgtcat ccaggcggta cacagccatg ttcacggagt
     1441 cttccccgtt gttgcggctg ctgcccgtgt aggtttccgc gcccagctca atgcgggcaa
     1501 tgtcgtccag ccgggtaacg tggnnnnnnn nnnnagtcgt ccggtgacgt ttaccttata
     1561 ttggatgaag ttgttttccc cttccgagcc gatggagccg gccgcggcct gaacgttctg
     1621 cgccttgatg gcgttggaaa cgtcatccgg cgtgatattc agggcagaca tgcgcagggc
     1681 gtccagccag atgcgcatgc tgtaattctt ttccggcatc aggttgacgg cagagattcc
     1741 cggtacgcgg gcgacctcat ccttcaccct ggtcttgacg aagttgttca gctccaggga
     1801 actcatgctg gtggaccgga aagcgaagaa tcccaggatg tcagaggagc gtttcttgat
     1861 cttgatgccc tggtccgtca cctccttggg aagtttgtat tccacccgtt tcaaggcgtt
     1921 ctgaacgttc accatgttga tgtccgaatt cgtgccgctc ttgaacgtca gggacaggga
     1981 atacccgccg gtgttatctg acttggagga gaaatagagc aggttttcca acccgttgag
     2041 ttcttcctca atgggggagg ccagcgtttc catcaccacc tgggcgctgg cacccgtata
     2101 ggtggcctgc acgttgatgc tggtgggcgc gatttccgga tactccgcaa tggggagctg
     2161 gttcaggcag atgccgccag ccagcatcat caggatggcg atgacgatgg cgaatttggg
     2221 gcgtttgata aacaggtccg caatcatggg tagctcagtt ttgaacggaa tataccggat
     2281 tcaccttggc gccgggacgc accttgtgca tgccgtcgat gatgacgact tccccttcct
     2341 tgacgccgga actgaaacac tgttcgttat tgaccgtgtt gcccagcgta acgtcccggc
     2401 gttccacgac gccctggtcg ttgacgatgt aaacgtagct cttgtaggca tcgtgcatca
     2461 cggaggaaat gttggcagcc gggaagcggt ccacgttttt cttggaaaga ttaacggtga
     2521 ctacgccgcc gggcgtcagc acgtcttccg gattggggaa ttttgcccag acattcaggg
     2581 tgtccgtggt cgtctggacg gtattgtccg tcatgaaaat tctgcccggc tggtcgtatg
     2641 ccttgccgtt ggcgaggttg acggacacaa gggcgtcttt cttcatattt tcctgggtac
     2701 cgaacaggga aaggaaatcg cgttcactga tagggaactt cacgtacact tcatcgatgc
     2761 ctgtaatggt caccagggaa ccggaggtgg gggtgatgta attgcccgtg gaaaaggtga
     2821 cgcggcctgt gcggcccgtg atgggagccg tgagcctggt atagttaaga ttgtccttcg
     2881 ctaccgtaat ggcggcgttt gcggactgga tttccgcttc cagggcatgc atggtgctct
     2941 tggcgttttc caccgtgtcc atggagacgg atttggaggc cagcaacctg ttatagcgtt
     3001 caaaactatt ttttgcgtaa agcagtttgg cttcaagctg ggcctttttg gctaccgctt
     3061 cctccacagc cgccctgtag cgcgtatcct caatttcgaa caggagatcc cccttcttca
     3121 cgacctttcc ttcctggaag cgggtggcta cgatgttgcc ggagacgcgg ggctgcacag
     3181 tcacccggtc aatggcttcc acctggccga tgtacttctt gttcaccgcg ctgtcaatag
     3241 cggcggcttt ctggacgagt acggtactgg gtttggctgc agcccctccc ggagttcctt
     3301 gggaataacc cggaacaaac agggaaaaaa gcaggccgga aacggccagc aggcggaggg
     3361 gcgccttccg atgatgcgtg tgtttt
//
LOCUS       DAOMSU010000232         3384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2810_length_3384_cov_3.091919, whole genome shotgun sequence.
ACCESSION   DAOMSU010000232 DAOMSU010000000
VERSION     DAOMSU010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2810_length_3384_cov_3.091919"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /gene="rho"
                     /locus_tag="PK077_08775"
     CDS             <1..1476
                     /gene="rho"
                     /locus_tag="PK077_08775"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRN24431.1"
                     /translation="PPDLLRQQTQRLYQHFFMTEELDNTPSPAGDIPQETLPKPLPAP
                     EETAGEQPAAAPEENSGNAVREEEEAAPVLEQIDINELRKRPLNDLQEMAEGLPIRNA
                     ASLTKSQLVFELGKQLLAKGHEVVVSGVMEQAKDNYAMLRDAVKSFRTSPDDIYLGGN
                     LIKPLHLRVGQQIKVRLRKLRPHDKYLSAASVISVEDIPAEDYRARSDFERLTPLFPK
                     ERLLLENKGVNSAAMRVLDLMTPFGKGQRGLIVAPPRGGKTVLLKTIARSIRANYPEV
                     ELIVLLLDERPEEVTDFEETVDAPVFASTFDEPSRRHAQVSDLVIERAKRLVEMGRDV
                     VILLDSLTRLARGYNANQTGGRIMSGGLGSNALEKPRKFFSAARNVEEGGSLTIIATC
                     LVDTESRMDEVIFEEFKGTGNLEIRLDRELSERRIYPAISLSQSGTRNDDRLYNEQEF
                     VKIMQLRRQLAMKPGWEGLQALLQNISKTQNNAELLLTGLR"
     gene            complement(1642..>3384)
                     /locus_tag="PK077_08780"
     CDS             complement(1642..>3384)
                     /locus_tag="PK077_08780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRN24432.1"
                     /translation="YVGSYLLIGWDVLKAAFLGMMRGRAINENFLMSIASLGAMFLGD
                     YSEAVGVMLFYRVGEYLQERAVGSSRRSVSELMSLRPEAVHVKKAGAVDDVPPSEVLP
                     GSLIEVRPGERVPLDGVVTGGFSVLDTSAMTGESLPVEAGSGSPVLAGYINGQGVLEV
                     RTERDWRHSALARVQELVEAASGRKSPLEGRLSSFSRIYTPLVISIAALVFLLYPLVT
                     GGSWADGLFRALVLLVISCPCALVLSIPLGFFAGIGRAARQGILLKGSNYLDALRKVK
                     TVVFDKTGTLTEGVFSVDEVLPRDGVSPEELLYWAAHAELSASHPLGRSIVKAYEGTL
                     FPDRVAELVEVTGGGVSARVEGRPVLVGKKSFLQEAGVRTEEGEDHGVTVYAALDGIL
                     LGCLRLSDRVKPGAERAVRKLRELGVSNLVMLTGDSSSAGTEVGLKLGMDGVFCGLMP
                     AGKLEHVRRLKPETGLLAFVGDGMNDAPSLAAADIGIAMGGVGSDTALQAADVVVMKG
                     DPLAVPLGMMLSQATERIIVQNIVLILGVKILVMVLGILGLAGMWAAVMADVGVCLLA
                     VGNSMRIFRVKLDM"
BASE COUNT          786 a         1110 c          883 g          605 t
ORIGIN      
        1 cccccggacc tgctccgcca gcaaacccaa agactttacc aacatttttt catgactgaa
       61 gaactcgaca acaccccttc ccccgccgga gatattccgc aggaaaccct ccccaagccc
      121 ctgcccgcgc cggaagaaac cgccggggaa cagccggctg ccgctccgga ggaaaatagt
      181 ggaaacgcag tccgggagga ggaggaagca gcccctgttc tggagcagat tgacatcaat
      241 gaactgcgga aacgccccct gaacgatctt caggaaatgg cggaagggct gcccatccgg
      301 aacgcggcct ccctcaccaa gtcccagctg gtttttgaat tggggaaaca gcttctggca
      361 aagggccatg aagtcgtagt ttccggggtc atggaacaag ccaaggataa ttacgccatg
      421 ctgagggatg cggtgaaaag tttccgcacc tctccggatg atatttatct gggcggcaat
      481 ctcatcaaac ccctgcatct ccgcgtaggc cagcagatca aggtcaggct gcgcaaattg
      541 cggcctcatg acaagtacct ttctgcagcc tccgtcatca gcgtggagga catccctgcg
      601 gaagactacc gggcgcgcag cgattttgaa cgcctcaccc ccctcttccc caaggaacgc
      661 ctccttctgg aaaacaaggg ggtcaattcc gccgccatgc gcgtactgga cctcatgacg
      721 cccttcggca aagggcagcg cggcctgatt gtggcccccc cgcgcggagg aaaaaccgtt
      781 cttctgaaaa caatcgcccg ttccatcagg gccaattatc cggaagtgga actgattgtg
      841 ctgttgctgg acgaacgtcc ggaggaagta acggattttg aagaaaccgt ggatgctccg
      901 gtattcgcct ccacttttga cgaaccttcc cggcgccatg cccaggtttc cgatctggtt
      961 atcgaacggg ccaaacgcct ggtggaaatg ggcagagacg tcgtcatcct gctggattcc
     1021 ctcaccaggc tggcccgcgg ctacaatgcc aaccagacgg gaggacgcat catgtccggc
     1081 ggcctggggt ccaatgcatt ggaaaaaccg cgcaaattct tttccgcggc gcgcaatgtg
     1141 gaagaaggag gcagcctgac catcatcgcc acatgcctgg tagacacgga atcaagaatg
     1201 gacgaagtga tttttgaaga attcaaggga acgggcaatc tggaaatccg cctggaccgg
     1261 gaactttccg aacggcgcat ttatccggcc atttcccttt cccagagcgg cacccgcaat
     1321 gacgacaggc tgtataacga acaggaattc gtcaaaatca tgcaattgcg ccgccagctc
     1381 gccatgaaac cgggatggga aggccttcag gctctcctgc aaaatatctc caagacacag
     1441 aataacgcgg aacttctgct gacggggctg cggtaagatt ttctgcccgg cctgtttttc
     1501 ccccgtcctc cgttccgccc gccggcacgg aggttttttt atcaggatat cttccgctgt
     1561 tccaagattc cgcggcagca tccggatatc gggatggggg catcctccca taaacagggg
     1621 gaggaagaga gttccctttc gtcacatgtc cagcttcacc cggaaaatac gcatggagtt
     1681 gcccaccgca agcaggcaga cgcccacatc cgccatcacg gcggcccaca tccccgccag
     1741 ccccagaata cccagcacca tgaccaggat tttgacgccc aaaataagaa cgatgttctg
     1801 cacaatgatg cgctctgtgg cttgggaaag catcatcccc agcggaacgg ccaaaggatc
     1861 tcccttcatc accactacat cagccgcctg aagagccgta tcggacccta caccgcccat
     1921 ggcaatgccg atgtcggcag cagcgaggga aggggcgtca ttcataccgt cccccacaaa
     1981 ggcaagcagc cccgtttcag gtttcagccg gcgcacatgc tccagcttgc cggcaggcat
     2041 cagcccgcag aataccccgt ccatccccag cttaagcccc acttccgttc cggcggaaga
     2101 agaatccccc gtcagcatga ccaggttgga aacgcccagt tccctcaatt tccgcaccgc
     2161 acgctccgct cccggcttga cgcggtcaga caggcggagg catcccagaa gtatgccgtc
     2221 caacgccgca taaacggtca cgccatggtc ttctccctcc tccgtcctta cgccggcctc
     2281 ttgaagaaaa gactttttcc ccaccagaac cggtctccct tccacgcggg ctgaaacgcc
     2341 gccgcccgtc acctctacca gttccgccac gcggtccgga aacagagtcc cttcatatgc
     2401 cttcacaatg gaacgcccca gcggatggga ggcagagagt tccgcatggg ccgcccagta
     2461 cagcagttcc tccggggaaa cgccgtcacg gggaagcact tcatccacgg aaaaaacgcc
     2521 ttccgtcaag gttccggttt tgtcaaacac caccgtcttc accttccgca gggcatccag
     2581 atagttgctc cctttcagca aaatcccctg ccgcgccgct ctccctattc cggcaaaaaa
     2641 gcccagcggg atggacagta ccagcgcgca ggggcaggaa atcaccagca ggaccagggc
     2701 gcggaacaga ccgtccgccc agcttccgcc cgtcacaagg ggataaagca aaaataccag
     2761 agcggctatg gaaatcacca gcggcgtata aatgcgggaa aatgaggaca gcctcccctc
     2821 cagaggggat ttgcggcctg atgcggcttc caccagttcc tggacccgcg ccagggcgga
     2881 atgccgccag tcccgctccg tacgcacttc cagcacaccc tgcccgttga tgtaccccgc
     2941 cagcacgggg cttccggatc cggcttccac cggcagggat tccccggtca tggcggaggt
     3001 atccagcacg gagaaacctc ccgttaccac accgtccaac ggaacgcgtt cgccggggcg
     3061 cacttcaatc agggagccgg gcagaacctc ggaaggagga acgtcatcta cggctccggc
     3121 ttttttcaca tggacggcct caggcctcag actcatcagt tcgcttacgg accgacggga
     3181 gctgcccacc gcacgttcct gaaggtactc tcccacgcgg tagaacagca tcacccccac
     3241 cgcctcggaa taatccccca ggaacatcgc ccccagggaa gcaatactca tcagaaaatt
     3301 ttcattgatg gcgcggccgc gcatcatccc cagaaaggcg gccttcagta catcccagcc
     3361 tatcagcagg taggagccca cgta
//
LOCUS       DAOMSU010000233         3370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2824_length_3370_cov_2.230769, whole genome shotgun sequence.
ACCESSION   DAOMSU010000233 DAOMSU010000000
VERSION     DAOMSU010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_3370_cov_2.230769"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /gene="thiC"
                     /locus_tag="PK077_08785"
     CDS             <1..1462
                     /gene="thiC"
                     /locus_tag="PK077_08785"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HRN24433.1"
                     /translation="VAIRENLGREQAFKAIYDRYPNAKSRPDEAAEALETLTMMPRPS
                     ELEAQEGFGPSSMVARDRLDYQHAPERRNGCRMPAYFTPEFVRDEIASGRALIPANIN
                     HPECEPMAIGRNFLVKINANIGNSALGSSIEEEVEKLRWAIHWGADTVMDLSTGKNIH
                     ATREWILRNSPVPIGTVPIYQALEKVGGKVADLSWEVFRDTLLEQARQGVDYVTVHAA
                     LLLRFVNHTARRMTGIVSRGGSIMAQWSMIHEQENFLYSHWDEICSILAAYDIAVSIG
                     DGLRPGSVADANDFAQLAELEVQGDLTMRAWKAGVQVMNEGPGHVPMHLIGENMSKQL
                     EWCMEAPFYTLGPLVTDIAPGYDHITGAIGGAIIGQLGCAMLCYVTRKEHLGLPDRED
                     VREGVVTYKLAAHAADLAKGHPSAQWRDNALAQARFEFRWEDQFNLSLDPQKARSFHD
                     LTLPHANAKKAHFCSMCGPDFCAMRLSQDIRRRSQQ"
     gene            1562..2506
                     /locus_tag="PK077_08790"
     CDS             1562..2506
                     /locus_tag="PK077_08790"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRN24434.1"
                     /translation="MRNSFFPAIGVLSLASALICSASSSWETDWNKALEKAGKGGHPV
                     LADFTGSDWCPGCIHLRKNIFDTDAFAKYAADHQFVLLELDFPKAAGKMPPEQLKFHE
                     ELMRRYGVSSFPSVLLMEGNGAPYAKIVGATRTPEEYLKKLEAAGETRRKLKEAVAAA
                     QPLKGKEKLEQLVKALNVLPEDLQPFQKGLIAEISALDPEDRYGFGKKSEKAAAMEKQ
                     RLMWEQFCQKYSGRLSAEETRAGREEALQMLEKKDTLPPIRLKVAKYISDGYVLERNL
                     PKALEYLEIARDADPESQAAKKLEPWIDNMRKHINQEK"
     gene            2638..3216
                     /locus_tag="PK077_08795"
     CDS             2638..3216
                     /locus_tag="PK077_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRN24435.1"
                     /translation="MNTPAYVRNEEVDEESSHLLSWDEWLREHASQLLLFARQQSRSP
                     EDAEDILQDALVRLARKEASGEFVGGQEAWLSYVFASIRRLAVDYGRKSDRRQKREDE
                     ACASERDEDVYTDPWFSSAAADEELKQFMEMQLRKLPSKFSEVIVLKIWGEQTFQQIA
                     ETLEISQNTAASRYRYGIDLLRRALRNKKNQF"
BASE COUNT          788 a          854 c          997 g          731 t
ORIGIN      
        1 tgtcgccatc cgggaaaatt tgggcaggga acaggctttc aaggcgatat acgaccgcta
       61 tccgaatgcc aagagccgcc cggacgaagc tgcggaagcc ctggaaacgc tcaccatgat
      121 gccacgtccg tccgaattgg aggcgcagga gggatttggg ccgtccagca tggtggcccg
      181 cgaccgcctg gattaccagc atgccccgga acgccggaac ggctgccgca tgcctgccta
      241 ctttacgccg gaattcgtca gggatgaaat tgcctccggc cgtgcgctga ttcccgccaa
      301 catcaatcat ccggaatgtg agccgatggc tatcggccgc aatttcctgg taaaaatcaa
      361 cgccaacata ggcaactccg cgctggggtc cagcattgag gaggaggtgg aaaagctgcg
      421 ctgggccatt cactgggggg cggataccgt tatggacctg tccaccggga agaatatcca
      481 cgcgacgagg gaatggattt taagaaactc ccctgtcccc atcggtactg ttcccattta
      541 ccaggctctg gaaaaggtgg gaggaaaggt ggctgacctg agttgggagg ttttccgcga
      601 taccctgctt gaacaggccc gccagggcgt ggactatgtg acggtgcacg ccgcccttct
      661 gctcaggttc gtgaaccata cggcgcggcg catgacgggc atcgtttccc gcggcggttc
      721 catcatggcg cagtggagca tgatccacga acaggaaaat ttcctttatt cccattggga
      781 tgaaatttgt tccattctgg cggcttacga tattgccgtc tccatcgggg atggcctgcg
      841 gcccggttcc gtggcggacg ccaacgactt cgcccagctg gcggagctgg aagtgcaggg
      901 ggatttgacc atgcgcgcgt ggaaagcggg cgtgcaggtc atgaatgaag gccccggcca
      961 cgttcccatg caccttattg gagagaacat gagcaagcag ttggaatggt gcatggaagc
     1021 gcccttttac acgctggggc cgctggtaac ggacatcgcc cccggttacg accatattac
     1081 cggagctatc ggaggggcga ttatcggcca gcttggctgc gccatgcttt gctatgtaac
     1141 cagaaaagag catctggggc ttcctgaccg ggaggatgtg agggaaggcg tggttaccta
     1201 caagctggcg gcccacgccg cagacctggc gaaggggcat ccatccgcgc aatggaggga
     1261 taatgccctg gcccaggcca ggtttgaatt ccgctgggag gatcagttca acctttccct
     1321 ggatccgcaa aaggctcgtt ccttccatga cctgacgctt ccccatgcca acgccaaaaa
     1381 agcccatttc tgttccatgt gcggtccgga cttctgcgcc atgcgcttga gccaggatat
     1441 ccgccgccgc tcacagcaat aggcgtcttt tctccggcat ttcggggggc ttgcggttcc
     1501 gagggggcca gcccttaaaa aagaatgaca agccccggta tcctgctata gtacggttaa
     1561 gatgagaaat tcattttttc ctgcaattgg tgttctttcc ttggcctccg ctttgatttg
     1621 ttctgcgtct tcctcctggg agactgattg gaataaagcg ctggagaagg ccggaaaggg
     1681 cggacatcct gtgctggctg attttaccgg ttccgactgg tgtcccggat gcattcacct
     1741 gcgcaaaaat atttttgaca cggatgcgtt cgccaaatat gcggcggatc atcaattcgt
     1801 gctgctggaa ctggatttcc ccaaggctgc cgggaaaatg ccgccggaac agttaaaatt
     1861 ccatgaagag ctgatgcggc gttatggcgt ttcctcgttc ccatccgttc tgttgatgga
     1921 aggaaatggc gctccctacg ctaaaatagt gggtgccacc agaactccgg aggaatatct
     1981 gaaaaaactg gaggctgccg gagaaacgag gaggaagttg aaagaggccg tagcggcggc
     2041 ccaaccattg aaaggaaagg aaaaactgga gcaactggtt aaggccttga acgtgcttcc
     2101 ggaagatttg cagcctttcc agaaggggtt gattgcagaa atttccgctc tggacccgga
     2161 ggacagatac ggttttggaa agaagtctga aaaagccgca gccatggaga agcagcggct
     2221 tatgtgggaa cagttctgcc aaaaatattc ggggaggctc tccgcagaag aaacgcgcgc
     2281 cggccgggag gaagcattgc agatgttgga gaaaaaggat acgcttcctc ccatccgcct
     2341 gaaggtcgcc aaatatatca gtgatgggta tgtcttggaa cgtaatttgc ccaaagcttt
     2401 ggaatacctg gaaattgccc gtgatgccga tccggagtct caagccgcca aaaaactgga
     2461 accgtggatt gacaatatgc ggaaacatat caatcaggag aagtaaaaat tgttccgaaa
     2521 ggcatggccg tcttccgtcg aagggggagg gccatgcctt tccgtttttt attttaaaca
     2581 aaaaaacagg gctgttgcgc tatggctgat gaaggaaccg aatgtacgga tgcagaaatg
     2641 aatacgccgg cttacgtcag gaatgaggag gttgatgaag aaagcagcca tttgctgagt
     2701 tgggatgagt ggctgcggga acatgcttcc cagctgcttc tgtttgcacg ccagcagtcc
     2761 cgctcaccgg aagatgcgga ggatatactt caggatgccc tggtgcgttt ggctcgcaag
     2821 gaggcttccg gggaatttgt gggtgggcag gaggcgtggc tttcctatgt ctttgcctcc
     2881 atccggcgtc tggcggtgga ttatggccgc aaatccgacc gtcgccagaa aagggaggat
     2941 gaagcctgcg cctctgaacg ggacgaagat gtttatacgg acccctggtt ttccagtgct
     3001 gcggcagatg aggaactcaa acagttcatg gaaatgcagc tcaggaagct cccttccaaa
     3061 ttctccgagg tcattgtctt gaaaatatgg ggggaacaaa cttttcagca aattgcggaa
     3121 acgctggaaa tttcgcagaa tacggcggcg agccggtacc gttacggtat tgacttgctc
     3181 cgcagggctt tgagaaataa aaagaatcaa ttctaatgcc gctctaccct tatgaaccat
     3241 gaaacaacat catccgccgc aggggaggaa gacggcggta ctgccgtcct gcagcccctt
     3301 cccgtatctg atgaactgat ttcccggctg gccggaaagt gtgaggaaat cttccctttg
     3361 gagagccgtt
//
LOCUS       DAOMSU010000234         3364 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2835_length_3364_cov_2.880326, whole genome shotgun sequence.
ACCESSION   DAOMSU010000234 DAOMSU010000000
VERSION     DAOMSU010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2835_length_3364_cov_2.880326"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PK077_08800"
     CDS             complement(<1..935)
                     /locus_tag="PK077_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24436.1"
                     /translation="MTSPQELILKLQNLLTNASGADLQQFLAGLEQPGPAPAGDDLTP
                     DGRIKVAMPSKNGTTTPQWCERVAIALQRANAPIYNLAGSPVYITDDGKTVYLNPNNF
                     ISAAEKYICPCAFRSKDDSTLVYQPMKEPLAKLTLSSMEFLTAIPELIKIHDQITPAM
                     LPNGTYHLNQRGYDPESKIYTLKTAVDYDTEMPLEQALLIWRNWHKEFPFLDWSSSDL
                     QERATSATSRSFAVHTCACVALYASAMLPLSSPRLGYVYTSNSQRSGKSLLADLATGI
                     TYNNNAKHPWYYDDEKLQGVLNTILNTRAPDVYFD"
     gene            complement(932..2140)
                     /locus_tag="PK077_08805"
     CDS             complement(932..2140)
                     /locus_tag="PK077_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24437.1"
                     /translation="MDAITRAQKHIDTLEPAVSGSGGHAATFRACRILVNDYNLSLDE
                     AWPILLSFNARCEPPWNEKELRRKLEDAARRPKGNYGTDKSQGRKKPEPTTLPPSKRI
                     AKGPAKYNPPPKAPTGPPPVPQLDSSVIATLAEKGAPVLSRYFLANISATDPAIVTPD
                     LYLRALFDTARGEKTIIFADRKTQGQCLWPDQAKHIPTAAPDGIIFLGQPVDGFFRVR
                     DGKKSRRSSECVLTWRHALLESDQMDDVKQWLQALITLPLPIVSITFSGSRSLHVLFR
                     LEAATHEEWRGYVDQIKPALALIGADIQALTNHLVMPRLPGCYRTVNGQEKLQELLYF
                     NPRPTLRPLLYATPLRNVEQDWTTRAAEIVARPDEWSVPLIQQAAAACTTYGLNAALQ
                     SLTPLLPPKK"
     gene            complement(2143..2727)
                     /locus_tag="PK077_08810"
     CDS             complement(2143..2727)
                     /locus_tag="PK077_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24438.1"
                     /translation="MSSSPSVIHVYGDWLDYLLHAYGETQEQWNAPFDAAPILPELSK
                     ELYECGLNHHQLDVVKEEIADARLHILIGIFTIIFRNGYHPQRVTRNVYKLAKLYCPE
                     SVKNLSHSDLALLTSDTISASSMRAHACNAFLDSLTPAQAQALIDKYGKTALHKLAQV
                     SWTSRKAQKKRRRKKKQDTPAHRTRKHPELNLDL"
     gene            complement(2835..2909)
                     /locus_tag="PK077_08815"
     tRNA            complement(2835..2909)
                     /locus_tag="PK077_08815"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2875..2877),aa:Cys,seq:gca)
BASE COUNT          739 a          706 c         1085 g          834 t
ORIGIN      
        1 ttgtcaaaat agacgtcggg cgcgcgggtg ttaaggatgg tgttcaagac gccctgaagc
       61 ttctcgtcgt cataatacca tggatgtttg gcgttattgt tgtacgtgat gccggtggct
      121 aggtcagcca atagagattt gccggatcgc tgggaattgg aagtatagac atagccaaga
      181 cgcggcgaag acagggggag catggcggaa gcgtacaggg cgacgcaggc gcaggtgtgg
      241 accgcaaatg agcgggatgt ggcggatgtg gcccgctcct gaaggtcaga ggaagaccaa
      301 tccagaaagg ggaattcctt gtgccagttg cgccagataa gcaaggcttg ctccagcggc
      361 atttccgtat cgtagtccac ggcggttttc agggtgtaaa tcttactttc cggatcatag
      421 ccgcgctggt taaggtggta ggtgccatta gggagcatgg ccggagtgat ttggtcgtgg
      481 attttgatca attccgggat ggccgtgagg aattccattg atgaaagggt gagctttgcc
      541 agcggttcct tcatgggttg gtaaacgagc gttgaatcat ctttggagcg gaaagcgcac
      601 gggcagatat atttttccgc ggcggaaatg aaattgttgg ggttaaggta cacggttttg
      661 ccgtcgtctg tgatatacac aggagagccg gccagattat aaataggggc attggcgcgt
      721 tggagggcta tggcaacgcg ttcgcaccat tggggggttg tcgtgccatt tttggagggc
      781 atggcaactt tgattcttcc gtccggcgta aggtcgtctc cggcaggggc cggtcctggt
      841 tgctccagac cggccagaaa ttgctgcaga tccgctccgg aggcgttggt tagcaaattt
      901 tgtaatttga gaataagttc ttggggggaa gtcatttttt gggagggagt aagggggtga
      961 gagattggag ggcagcattg aggccgtagg tggtgcaggc tgcggcggct tgctggatca
     1021 agggcaccga ccattcatcc ggccgggcaa cgatttccgc ggcgcgggtt gtccagtcct
     1081 gttctacgtt gcggagcgga gtggcgtaga gtaagggacg cagggtaggg cgcgggttga
     1141 aatacaaaag ttcctgaagt ttttcctggc cgtttacggt gcgataacag ccgggcagcc
     1201 gcggcatgac aagatgattt gtgagggcct ggatgtctgc gccaatgagg gctaaggcgg
     1261 gctttatctg gtccacatac ccgcgccatt cttcgtgtgt ggctgcttcc aggcggaaga
     1321 gtacatgcag agatcgggat ccggagaagg tgatggagac aatggggagg ggtagagtaa
     1381 tgagtgcctg gagccattgc ttcacgtcgt ccatttgatc tgattccagc aaggcatgac
     1441 gccaggtgag gacgcattct gaagagcggc ggcttttttt cccgtccctg acgcggaaaa
     1501 atccgtccac gggctgccct aggaagataa tgccgtccgg cgcggccgtg ggaatgtgtt
     1561 tggcctggtc tggccagagg cattgacctt gcgtttttcg gtcggcaaaa atgattgttt
     1621 tttcaccgcg tgccgtatca aacagggcgc gtaaatacag atccggcgta actatggccg
     1681 gatctgtggc ggagatgttg gcgaggaaat agcgggataa tactggagcc cctttttccg
     1741 ccagggtggc aatgacggaa gaatcaagct gcgggactgg cggcggccct gtgggggcct
     1801 tgggtggcgg gttgtatttg gctggtccct tggctatgcg tttagagggg ggcaaggtag
     1861 tcggttccgg ttttttgcgt ccttgggatt tgtcggtgcc gtagttaccc ttggggcggc
     1921 gcgccgcgtc ttccaacttg cgccggagtt ccttttcatt ccaaggcggt tcgcaccggg
     1981 cgttaaatga taacaggata ggccaggctt catccaaaga tagattgtag tcgtttacaa
     2041 ggatgcggca tgcgcggaag gttgccgcat ggccgccaga accggaaaca gccggttcca
     2101 gggtatcaat atgtttttgt gcgcgggtga tggcgtccat ggtcaaaggt caagattgag
     2161 ttccgggtgt ttgcgtgtgc ggtgggcggg ggtgtcctgc ttctttttcc tgcgacgttt
     2221 cttttgggcc ttccggctgg tccaggaaac ttgtgcgagc ttgtgcaggg cggttttacc
     2281 gtatttgtcg ataagagctt gagcttgggc gggtgttaag gagtccagga aagcgttgca
     2341 ggcatgcgcc cgcatggatg atgcgctgat tgtgtcgctg gtcagcagag cgagatcgga
     2401 atgagacaag ttttttactg attccgggca gtatagtttg gcgagtttgt aaacattccg
     2461 ggtgacgcgt tgcgggtggt agccgttacg gaagatgata gtgaaaattc cgataaggat
     2521 atgtaggcgg gcgtccgcta tttcttcttt caccacgtca agctgatgat gattcaaacc
     2581 gcattcatag agttcttttg agagttcagg gaggatggga gcggcatcaa aaggcgcatt
     2641 ccattgttcc tgcgtttcgc cgtaggcgtg cagcaggtag tccagccaat cgccgtaaac
     2701 gtgtataaca gaggggctgg aactcataat gcaccctcct tccaagataa ggcttgctcc
     2761 aaaagtggaa ctaaaaacaa gatgtttact acatttttta ctacgctaaa ggcgtaaaat
     2821 gttgaaagta aacatggagg cgggagcggg aatcgaaccc gcgaataacg attttgcaga
     2881 ccgtcgcctt accacttggc taccccgccg ctgatgctgt gcgcggatac tagggaaaag
     2941 aaccgcgggt gtcaatctcg tttttggaaa agatgctttg tttttaagtg aaggactctc
     3001 tggaaacagc ctttttcttt ggcggaacgg acagagaagg agagaatgct tgttttttaa
     3061 ggcatcatga ttgtgcggcg gaatgccgcc cgtgcctgtt gtcagatcta tatggagcaa
     3121 ctttgttcca aaatggcgtg aatcaggata taaaaatgca ccaggagttt ttttgcaagt
     3181 tcacggcagc acgtaaaatt agaggggagg tcgttcctct tcgttccgaa tgagggggga
     3241 ctacgcgtta aacgtgttga tgggttcagg gaacgccgcc agaataccgg aaggctgatg
     3301 gttcagtctt tccaaaaagt aaacaacaac aatagcaatg aacaattata taccaatcaa
     3361 caag
//
LOCUS       DAOMSU010000235         3357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2847_length_3357_cov_2.468807, whole genome shotgun sequence.
ACCESSION   DAOMSU010000235 DAOMSU010000000
VERSION     DAOMSU010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2847_length_3357_cov_2.468807"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(308..1081)
                     /locus_tag="PK077_08820"
     CDS             complement(308..1081)
                     /locus_tag="PK077_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24439.1"
                     /translation="MSAALERLGRQAKALDFFDEITLFTEHDLSAEFTSRMGRYLTPS
                     CRGFGYWSWKPWSIHHVLQEMEEGDRLLYLDAGCHINANGTERFREYVNMLDRDSRGM
                     LVFTNGQPEYKWTKGDIFRHFGVSQEDGHITHTQQIAGGHVFLKKNPLTESLIRDWLH
                     VFYDHLHLADNTPSASPNLPGFVENRYDQSIFSILCKLRGITTLDGSETYAENWEQLS
                     LFPFQDRRDMGIPRAGKKGWLEKCRQLLLSSKRNGLGIR"
     gene            complement(1142..>1980)
                     /locus_tag="PK077_08825"
     CDS             complement(1142..>1980)
                     /locus_tag="PK077_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24440.1"
                     /translation="ALEYDADIVECGNHVFEDSSDRLIEAKRRSPSRHFEENASPASF
                     FRDSIWGKMDICVWSKLFRKSMLDAHRLRFNVHLKSGAEDETFRLMAVPHASRLLFIP
                     DCLYYYRLMRNGSLSRRCNVPTYSKCVQEFQRLLYIVDYWQKQGWQNEGLFAYGVRKI
                     RPFFVSKHPLFHQMTAVQQRSALDWWSQFYQKVEGKRFLSALSGRDRQLADLLNSAEP
                     VPNGWGRILLAACSLLPGQKGRYYSCKKMLAEHFSQVCPNGFQKENASLEEPFDTSPP
                     SL"
     assembly_gap    1981..2026
                     /estimated_length=46
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2027..2325)
                     /locus_tag="PK077_08830"
     CDS             complement(<2027..2325)
                     /locus_tag="PK077_08830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN24441.1"
                     /translation="MIPDVSIIVPCYNVAAYVDSCLDSLVRQTLRNIEIICINDGSTD
                     ETWIHLLRWKEKDSRIILLNQRNAGVSAARNAGLDAARGLYVGFADPDDYMDPEM"
     gene            2629..>3357
                     /gene="rfaD"
                     /locus_tag="PK077_08835"
     CDS             2629..>3357
                     /gene="rfaD"
                     /locus_tag="PK077_08835"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HRN24442.1"
                     /translation="MIIVTGGAGFIGSNIVAALEKAGKKDLVVVDELGSSDKWRNIAK
                     RELCAVVSPEGMLDYMKAHADEVEAVIHMGAISATTETDADLIIRTNFTLSWHLWEFC
                     SLYGKRFIYASSAATYGDGSMGFDDDASLEHVNALRPLNAYGWSKALFDRKVAREVKE
                     GRPVPPQYAGLKFFNVYGPNEYHKGGQKSVVAHIFPQVKANETVKLFKSYHPDYRDGW
                     QLRDFVWVGDCVDVVLWLLEHPEVS"
BASE COUNT          745 a          784 c         1007 g          775 t
ORIGIN      
        1 gaatggggga aagccggatt ccggtgacag cataactgtg aataggggtg ggagaatgtc
       61 ggcggaggat agtgcgggtg cggagttttg gaatggaggg atgtttgctt tcatgaaggc
      121 aaaacattcc tgatcaaatg aaaagagctt gtccaaataa ccgtcattac attccggaaa
      181 cggcagccga aggggaaagg aattctcggg atgaagctgt ggaaaaggac gggaaaatag
      241 ttcatggtgc gtcagattgc cccgtttgga aacgtcgtgc agcccggttg ttccgaatgg
      301 ggaagcttta ccggattcct aatccgtttc ttttggaaga aagaaggagc tgcctgcatt
      361 tttccagcca tccctttttc ccggcccgtg ggattcccat gtcccgtctg tcctggaatg
      421 gaaacaagga gagctgttcc cagttttccg cataggtttc cgatccatcg agggtagtaa
      481 tgcccctcag tttgcatagg atggaaaaga tgctctggtc ataccggttt tccacaaaac
      541 ccggcaggtt gggggaggcg gaaggagtgt tgtccgcaag atgcagatgg tcgtaaaaga
      601 catggagcca atcccggatc aggctttccg tgagcgggtt ctttttcagg aaaacatggc
      661 cgcccgcaat ttgctgggta tgcgtgatat gcccgtcttc ttgggaaacg ccgaagtggc
      721 ggaagatatc tcctttggtc catttgtatt ccggctgtcc gttggtgaaa accagcatgc
      781 cgcggctgtc gcggtccagc atgtttacgt attcccggaa gcgctccgtt ccgttggcgt
      841 tgatatggca gcctgcatcc agataaagga ggcggtcgcc ttcctccatt tcttggagga
      901 catggtggat ggaccagggt ttccaggacc agtatccgaa tccccggcag gacggcgtca
      961 ggtaccggcc catgcggctg gtgaattctg cggaaaggtc gtgttccgtg aagagcgtga
     1021 tctcatcaaa aaaatccagc gctttcgcct gcctgcccag ccgctccagg gcggccgaca
     1081 tgcgggagtc tgcaaatgag agaaaacggt tcattgatcc ggaatgggag gataaggcaa
     1141 tttacaggga aggcggcgat gtgtcaaacg gctcttccag agaagcgttt tctttttgaa
     1201 acccattggg gcagacttgg gaaaaatgtt ccgcaagcat ttttttgcag gagtaataac
     1261 gtcctttttg tccgggaagc agggagcagg ctgccagcag aatgcgcccc cagccgttgg
     1321 ggaccggttc cgccgagttc aacaggtccg ccagttgcct gtcccgtccc gatagagcgg
     1381 agaggaaacg ttttccttcc accttctgat agaactggct ccaccagtcc agcgcggaac
     1441 gttgctggac agcagtcatc tgatggaaaa ggggatgctt ggaaacaaaa aaaggcctga
     1501 ttttccgaac gccgtaagcg aacaggcctt cattctgcca tccttgtttc tgccagtagt
     1561 ccacaatgta cagcagccgc tggaattcct gcacgcattt ggagtaggtg ggaacgttgc
     1621 agcggcggga gagggagccg ttgcgcataa ggcggtagta atacaggcag tcgggaatga
     1681 acaggagacg ggaggcatgg ggaacggcca tcagccggaa ggtttcatcc tccgcaccgg
     1741 atttcagatg tacgttgaag cggaggcggt gggcgtccag catgcttttc cggaacagtt
     1801 tgctccacac gcagatatcc attttccccc agatggaatc ccggaagaag ctggccggag
     1861 aggcgttctc ttcaaaatgc cgggagggtg atcttctttt ggcttcgata agccggtctg
     1921 acgagtcttc aaaaacatgg tttccgcatt ccacaatgtc tgcgtcatat tccagagccg
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntaca tttccggatc
     2041 catgtagtcg tccggatccg caaagccgac ataaaggccg cgggcggcat ccagccctgc
     2101 attcctggct gcggagacgc ccgcgttccg ctggttcaga aggatgattc tgctgtcctt
     2161 ttctttccag cgcagcagat gtatccaggt ctcatccgtg gagccgtcgt tgatgcagat
     2221 aatttcaatg ttccggagag tctggcgtac caggctatcc aggcagctgt ccacgtaggc
     2281 ggctacattg tagcagggaa caatgatgga aacatcgggg atcatagggg gtgggaatgt
     2341 gtgtaacgga tttacaatcc gtggtcaatg ataatttttg caagacaggt cttaccgaaa
     2401 gtttttaacg ttgccagtat gaaaagagga tttgaggggc gcatcctccg caggctgcgc
     2461 ggggattgcg ttccaatggt tttccgcatg gaacggtcag ggtttggagt gtccggattg
     2521 taaattcgtt gaaaattggg agccggggaa cgcctcaaat ggcagttgcc tagggacggg
     2581 tggatgtggt acaaaaggcc gtacaacttt ttcattgatt cttctcttat gattattgtc
     2641 acgggtgggg ccgggtttat cggctcaaac attgtcgccg ctctggaaaa agccggtaaa
     2701 aaggatcttg tggtggtgga cgagctgggc tcttccgaca agtggaggaa cattgccaag
     2761 agagagctgt gcgccgtggt ttctccggaa ggcatgcttg attatatgaa ggcgcatgcg
     2821 gatgaggtgg aggcggtcat tcacatgggg gccatttccg ccaccacgga gacggacgcc
     2881 gatttgatta tccggacgaa tttcacgctg agctggcatt tgtgggagtt ttgttccctg
     2941 tacggcaaac ggtttattta cgcctcctcc gcagccacgt acggggacgg ttccatgggg
     3001 tttgacgatg acgcctccct ggagcacgtc aacgcgctgc gccccctgaa cgcatacggc
     3061 tggagcaagg ccctgtttga ccgtaaagtg gcccgggaag tgaaagaagg gcgtccggtt
     3121 cccccccaat acgccgggct taaatttttc aacgtgtacg gccctaatga ataccataag
     3181 ggcgggcaga aaagcgtggt ggcccatata ttcccccagg tgaaggccaa tgaaacggtg
     3241 aagctgttca aatcttacca tcccgattac cgtgacggat ggcagctccg ggatttcgtg
     3301 tgggtgggcg actgcgtaga tgtggtgctt tggctgctgg agcatccgga ggtgagc
//
LOCUS       DAOMSU010000236         3354 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2852_length_3354_cov_2.472264, whole genome shotgun sequence.
ACCESSION   DAOMSU010000236 DAOMSU010000000
VERSION     DAOMSU010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2852_length_3354_cov_2.472264"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="PK077_08840"
     CDS             complement(<1..882)
                     /locus_tag="PK077_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24443.1"
                     /translation="MSASKLRRLVKGCRELEQLAKSCRLQNGVGYTFNRGLGGTSLTI
                     RPTGGRNKAGDGEPFTLKRLEKGDAGYKAYFWPGMVFEVHPGGVRRIKPELNGGKMDQ
                     AEEPPFLSVQGGDKVFLYLERSADNHDCITYAEVTAEEIGLARAVRIYLGEFEEETDE
                     AGEKVLKYHEAWSGHVHYAQSSLNEGWRVVVDTDEEGAPDMAYVKKGDIYIAGQLAQR
                     GGGTWEVAPKEEGEIWLEVKCTVDGVIKSAELKETKGSSKPLQYVAEPDDEEAEEEFA
                     YCFLLAKVEKLEEPLQED"
     gene            complement(922..1653)
                     /locus_tag="PK077_08845"
     CDS             complement(922..1653)
                     /locus_tag="PK077_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24444.1"
                     /translation="MGRKINIKKRETHEKTLEIERGDEGEVRAVGRIVYTDNQEGWNT
                     RCPSIGSAYPDDAALRLKKISMEGMEGDMVRVTLYYELPRETSFEFGGGEEVEYSMDY
                     SCSEQPLLTHPNFQDIDGEEKDALMAMASGASPKDTFGKEDKVIEDVVKSEAGKKAME
                     KMRKGQVSFLCPGGVFSVTSTVQALSMAGVGKKGAPGSGAPAVSGKYDWIKEGVSGRR
                     TGTGNWRQTVSWRLSGPDGWDSDLY"
     gene            complement(1689..>3354)
                     /locus_tag="PK077_08850"
     CDS             complement(1689..>3354)
                     /locus_tag="PK077_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24445.1"
                     /translation="APIISVVGKGLATVAAGGDRLHGMLRNGILAWVAWKAAGSSAGA
                     AVGQAVLSAGRTWQTGFNNALVLSGQKTRSVMGDIQALGACARSQAARMGAAFKGVGV
                     SLASSLKGPAIMGAIAAISVAGEKLYKAWRDARGVVPKEEMDKKKNFKRANDDFDERV
                     WKMAGEASSKLDVGRVMDEYDAEIKRLKREEEDLLAEDPLGRMTVAVQDRLVLLQRER
                     KELQQVAEANAKAAETRERAAQRGQQTEEARKKTLEKIREIQDELLSLDYDRAEEERE
                     RRRSGMGLEDRKKDLLGGYGSIEGLKKAIAEQKALLDGGDAVDGMLNLEGVESRIKSL
                     YELLGKVEEVDREIVERNKEWDKAEAKHQKQAALLRAEIHGQKDKLRVLQEQARVLEL
                     QNQYEADGMSKARAGAAAREIAALEQNRNRAQAGREYRRQMALLKAQAEGNKAEERRL
                     KMAERMKEIYDQQRGLGIDRKTAMRRARGMAGLEDMVERRKDRKEGSGPIADSLAQVG
                     GGGRSMMGSMPQLTEARKQTNLLQQIVKNTGAGRRGTLKTAAVLGY"
BASE COUNT          610 a         1258 c          625 g          861 t
ORIGIN      
        1 atcctcctgc aagggttctt caagcttctc caccttcgcc aaaaggaagc aataggcgaa
       61 ttcctcttcg gcttcttcat catccggttc cgctacatac tggagcggct tggaagatcc
      121 tttcgtttct ttcagttccg cacttttaat gacgccatcc acggtgcatt tcacttccag
      181 ccagatttcc ccctcttctt tcggtgccac ctcccaggta cctccgccgc gctgcgccag
      241 ttgcccggct atgtaaatat cgcccttctt tacataggcc atatccggcg cgccttcttc
      301 atccgtatca accacaaccc tccaaccctc attcagggaa ctttgagcat aatgaacatg
      361 gccgctccac gcctcatgat acttcaagac cttttctccg gcttcatccg tttcttcctc
      421 gaattcccca agataaattc tgaccgcgcg cgccagccct atttcctccg ccgtcacttc
      481 cgcatatgta atgcaatcat ggttatccgc gctccgctca agatacaaaa ataccttatc
      541 ccctccctgc acggacaaaa aaggcggttc ctccgcctga tccatcttcc ccccgttaag
      601 ttccggctta atgcgccgca cgcctcccgg atgcacttca aaaaccatgc cgggccagaa
      661 atacgcctta tatcccgcat cccctttttc cagcctcttc agcgtaaacg gctctccgtc
      721 tcctgctttg ttcctccccc ccgtcggcct tatggttaat gacgtgccgc ccagccctcg
      781 gttaaatgta taaccaaccc cgttctgcaa gcggcaagac ttggccaact gctccagctc
      841 ccggcacccc ttaaccagac gcctcaactt agacgcgctc aactcttccc cttgattaaa
      901 aaacggccag ctaatcataa atcaatataa atcagaatcc cagccatccg gaccgctcaa
      961 cctccaggaa accgtctgac gccaattccc ggttcccgtc ctgcggccgc tcacgccctc
     1021 tttgatccaa tcatattttc cgcttaccgc gggcgcgccg ctgcccggag cccctttttt
     1081 tccgaccccg gccatgctca acgcctgaac ggtagaagtg acggaaaaaa cccctccggg
     1141 acacaaaaaa ctaacctgcc ctttacgcat tttttccatg gccttcttcc cggcctccga
     1201 tttcacaaca tcctcaatca ccttatcctc tttcccaaac gtatctttag gagaagcccc
     1261 ggacgccata gccatcaatg cgtctttttc ttccccatct atgtcctgaa agttcggatg
     1321 cgtcagcaac ggctgttcag agcaggaata atccatggaa tattccactt cctcccctcc
     1381 accgaattca aaagacgttt cccgcggcaa ctcgtaatag agcgtcaccc tcaccatatc
     1441 cccctccatt ccttccatgc ttatcttttt gagcctcaaa gcggcatcat caggataagc
     1501 ggaccctatt gacgggcacc gggtattcca gccctcctga ttgtccgtgt aaacaatcct
     1561 ccccacagcc cttacttccc cttcatcccc ccgttctatt tccagcgtct tttcatgcgt
     1621 ttcccgcttc ttaatgttaa tttttcttcc catatttatt attctctctc tatcatttag
     1681 cggctatttc aatatcccag cacggccgcc gttttcaggg ttcccctccg ccccgcgccc
     1741 gtgtttttca cgatctgctg aagcaaattt gtctgcttcc tcgcttccgt aagttgcggc
     1801 atgctcccca tcatggagcg gcccccgccg cccacttgcg ccagactgtc cgctatgggt
     1861 ccgctccctt ccttccggtc cttccgccgc tccaccatat cctccaaccc ggccatgcca
     1921 cgggcacgcc tcatggccgt cttcctgtct atccccaagc cgcgctgctg gtcataaatt
     1981 tccttcatgc gctccgccat cttcagccgc cgctcttccg ccttgtttcc ctccgcctga
     2041 gctttcaaca gggccatttg ccggcggtat tcgcgcccgg cctgcgcccg gttcctgttc
     2101 tgctccaggg cggctatttc acgggccgcc gcgccggcgc gggccttgct catgccatcc
     2161 gcctcatatt ggttttgcag ctccagcacg cgcgcctgct cctgcaacac gcgcagctta
     2221 tccttctgcc cgtgaatttc cgcgcgcagc agggcggcct gcttctggtg tttggcttcc
     2281 gccttgtccc attccttatt ccgctccact atttcacgat ccacctcttc caccttgcca
     2341 agcaattcat acaggctctt gattctggac tccaccccct ccagattcaa catgccgtcc
     2401 acggcgtccc cgccatccag cagggctttc tgctccgcaa tggccttctt gaggccctca
     2461 atgctcccat atcctcccag caggtctttt ttccggtcct ccagccccat tccgctgcgc
     2521 cgcctctccc tctcttcttc cgcccggtca taatccaggg ataacaattc atcttgtatt
     2581 tccctgattt tctccagcgt cttcttccgt gcctcttccg tctgctgccc gcgctgcgcc
     2641 gcccgttccc gcgtctccgc cgctttcgcg ttcgcttccg ccacctgctg caactccttc
     2701 cgctcacgtt gcaacagcac cagcctatcc tgcaccgcaa ccgtcattct ccccagcgga
     2761 tcttccgcca gcaggtcttc ttcttcgcgc ttcaggcgtt taatttcagc gtcatattca
     2821 tccatgacgc gccccacgtc cagcttgctg gacgcctccc cggccatctt ccacacgcgt
     2881 tcatcaaaat catcattagc ccgtttgaaa tttttctttt tgtccatttc ttcctttggg
     2941 accaccccac gtgcatcacg ccacgccttg taaagctttt ccccggctac ggaaatagcc
     3001 gcaatagctc ccataatggc cggccccttc aagctggaag ccaaacttac ccccaccccc
     3061 ttaaacgcag cccccatccg cgccgcctgc gaacgagcac aagcccctaa tgcctgtata
     3121 tcccccatca cgctccgcgt cttctgcccg gataacacaa gcgcattatt aaatcccgtt
     3181 tgccacgtcc tccccgcaga cagcacagcc tgacctactg ctgccccggc ggaactgccg
     3241 gccgccttcc acgctaccca cgccaaaatt ccattccgga gcatcccgtg aagccgatcg
     3301 ccgcccgcgg ctaccgttgc cagccctttt cctactacgg aaataatagg cgcg
//
LOCUS       DAOMSU010000237         3339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2876_length_3339_cov_2.402558, whole genome shotgun sequence.
ACCESSION   DAOMSU010000237 DAOMSU010000000
VERSION     DAOMSU010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2876_length_3339_cov_2.402558"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="PK077_08855"
     CDS             complement(<1..506)
                     /locus_tag="PK077_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24446.1"
                     /translation="MKLFAFVMLSLVPSVTVCSVFSKEVDIYSGTYGNDDVEVFQPEE
                     VMYHLAGRDGVFYEGAFTFDNYNPDKGGAGNRQGVFSGYSTHFSSTEGTSLGFHCTGL
                     NPGKNSSASKSFIFVMGPWESGEEFVAPQVTFQNLGDLSFIADNMDMIGITDGATAGT
                     GGGRYGKA"
     gene            885..1745
                     /locus_tag="PK077_08860"
     CDS             885..1745
                     /locus_tag="PK077_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRN24447.1"
                     /translation="MTWSWNKLSAAKWEDAWSERIAGNPNAVITKIKGGKTVRITVYC
                     DDEEDALALKKYFGGTVREIKTQDWVATQKREKRPPLKIRDALVITEQSSTENLAALR
                     KQFPSRCILSVPAEMAFGTGDHATTSTCLRFICDAAKSRRKTSWSMTDIGCGTAVLAM
                     AALKLGAEHATAFDFDPMAIEVARHNMVRNAVTAEQLDLFVEDVFEWKPSESQQGDLV
                     VANLFSTILQKAFPQIIAAMKKDAALVISGILASQWEETKMAAERHGLFFERVVKRGK
                     WITAKGGIAR"
     gene            complement(1937..2542)
                     /locus_tag="PK077_08865"
     CDS             complement(1937..2542)
                     /locus_tag="PK077_08865"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HRN24448.1"
                     /translation="MFTGLVETTGTVEGFTPINGGARLTLTIPFGRELSLGDSVAVNG
                     CCLTVDALEGERISFDLLSQTLRVTSLGTLKPGMLVNLERAMSAMDRFGGHFVMGHVD
                     HTGTIAALEPVGQDHRLEVRIPEELTRYCIDKGSITIDGISLTIANLSGSLLEFWITP
                     HTFGRTNLQNAVPGQPVNLEVDMLAKYVERLFSAREQRAEG"
     gene            complement(2567..>3339)
                     /locus_tag="PK077_08870"
     CDS             complement(2567..>3339)
                     /locus_tag="PK077_08870"
                     /inference="COORDINATES: protein motif:HMM:NF019045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Na+/H+ antiporter"
                     /protein_id="HRN24449.1"
                     /translation="IGGTLTHFAAPPVVMVAEKWDWGLAHMIQHFGWKAVSAIVISNL
                     AYFLLFRREFARLASQQREFDEFDDAVPQSWEDRNDKIPLWVTLAHVCFLVWTVLFAH
                     EPVMFIGSFLFFIGFTLATPQYQNQMSLRVPIMVGFFLAGLVILGGVQAWWLEPVLTR
                     LGDYAMVGATLLTAFNDNAAVTFLASTVPNLPEAVKYSVVAGAVTGGGLTVIANAPNP
                     AGQAILGKYFKGINPLWLFAWAAFPTAIVFIFFTCFGH"
BASE COUNT          904 a          927 c          831 g          677 t
ORIGIN      
        1 tcagctttcc cgtacctccc gccaccggtt ccggccgtgg cgccatcggt aatgccgatc
       61 atgtccatat tatccgcaat gaagctcaaa tcgcccaaat tctgaaaagt aacctgagga
      121 gcgacaaact cctccccgct ttcccatggc cccatgacaa aaataaaaga tttggaagcg
      181 gaactgttct ttccgggatt aagcccggtg cagtgaaagc cgagagacgt tccttccgtg
      241 ctggaaaaat gcgtactata gccggagaaa acgccctgcc tgtttccggc gccccccttg
      301 tccggattat aattgtcaaa cgtaaacgca ccttcataaa agacgccatc ccttccggcc
      361 agatgataca tcacttcctc cggctggaat acttccacat catcgtttcc atatgttcct
      421 gaataaatat ccacttcctt ggaaaaaacg gaacagacag taactgaagg aaccaaggac
      481 aacatgacaa aagcaaataa tttcatgata atctgtacat ttggatgttt ttgcatatca
      541 aaagcactcc ttttaatgta cgaatttgga atccgttgtt attcgaacat caaaacaaca
      601 aaataacaat caatgaatta ttgttagaaa aaaagattgg aatagaacaa ggttccttcc
      661 tcttcttaca ggttggccat tgaatataaa aaaataatct ttactccgga ttccaaattc
      721 gcaaccatac tgaaaataaa atggtttgat agttgatgtc ttatattctt ctttgaataa
      781 atcaaatttt caacaataga gccccacagc cccaaaaaca gagggaatgc agttacaatc
      841 aatccattct tgcctccctg ccggaaaaat cccatacttt ctgcatgaca tggagttgga
      901 acaaactttc cgccgccaaa tgggaagacg cctggagcga acgcatcgcc ggaaatccga
      961 acgccgttat tacaaaaatc aaagggggga aaacggttcg aatcaccgtt tactgcgatg
     1021 atgaagaaga cgccctggcc ttgaaaaaat attttggagg aactgtcagg gaaatcaaga
     1081 cgcaggactg ggtagcgacc cagaagcggg aaaaacgccc tccgctcaaa ataagggacg
     1141 cactggtcat tacggagcag agttctacgg agaatctggc cgccctgcgg aaacagttcc
     1201 cctcgcgctg catcctgagc gtccccgccg aaatggcttt cggaacagga gaccacgcta
     1261 ccacatccac ctgtctgcgt ttcatctgcg acgctgccaa atcccgcaga aaaacatcat
     1321 ggagcatgac ggacataggc tgcggaaccg ccgtgctggc tatggcggcg cttaagctgg
     1381 gggcggagca cgccacggct ttcgattttg accccatggc cattgaagtg gcccggcaca
     1441 acatggtccg caacgccgtt acggcggaac agctggactt atttgtggaa gacgtcttcg
     1501 aatggaagcc ttcggaatcc cagcagggtg accttgtcgt cgccaacctg ttttcaacca
     1561 tactgcaaaa ggcttttcca caaatcatcg ccgccatgaa gaaggatgcc gctttggtca
     1621 tctccggcat tctggcttcc cagtgggaag aaacgaaaat ggcagcggaa cgtcacggtc
     1681 tgttctttga acgggtcgtc aaacgcggga aatggattac ggcaaaggga ggaatagccc
     1741 ggtagcaaat aaacccgctc cgcatttttg ttccgccctc acggccctgc cttcatcgga
     1801 acaacaatac ccggtttcct gttcaaaaat ctccgcatcc cggcacccct gctgcagaaa
     1861 acgggaatga actttcattg tccggcagga aaaaccagcc cgcatgcccg gcatgtgatt
     1921 ctccttacgg gcaggactat ccctccgcac gttgttcacg ggcagaaaac agtctctcta
     1981 catacttggc caacatatcc acttccagat tgacaggctg gccgggaacg gcattctgca
     2041 aattcgtgcg gccgaaagta tggggagtaa tccagaactc cagcagagag ccgctcaaat
     2101 tggctatggt cagggaaatg ccgtcaatgg tgatggaacc cttgtcaata caatagcggg
     2161 tgagttcctc cggaatgcgc acctccaaac ggtggtcctg ccctacaggc tccagagcgg
     2221 caatcgtacc ggtatggtcc acatgcccca tcacgaagtg gcctccgaaa cgatccatgg
     2281 cggacatggc gcgttccaga ttaaccagca tccccggctt gagggttccc agactggtga
     2341 cgcggagagt ctgggaaagc agatcaaagg agatgcgttc accctccagg gcatccaccg
     2401 tcaggcagca tccgttgacg gcaacggaat cccccaggga aagttcacgg ccgaacggaa
     2461 tggtcagcgt cagcctggct ccgccgttaa taggcgtaaa cccttccaca gttcctgttg
     2521 tttcaacgag tccggtaaac ataagaatga tagcacgctc cggccattaa tggccgaagc
     2581 aggtgaagaa aataaacacg atggcagtgg gaaaagccgc ccaggcaaac agccacaggg
     2641 gattgatgcc tttgaaatat ttacccagaa tggcctgccc ggccggattg ggggcgttgg
     2701 cgatgaccgt caggccgccg cccgtcacag caccagccac cacggaatat ttgacggctt
     2761 ccggcaggtt gggaaccgta gacgccagga aggtgacggc ggcattgtcg ttgaacgctg
     2821 tcagcagcgt agccccgacc atggcgtagt cccccagccg cgtcagcacc ggctccagcc
     2881 accaggcctg cacgccgccc agaatgacca gccccgccag gaagaacccc accatgatgg
     2941 gcacacgcag agacatttgg ttctggtatt gcggcgtagc cagtgtgaaa ccgataaaga
     3001 acaggaaact gccgatgaac atgaccggct catgggcaaa aagaaccgtc cagaccagga
     3061 agcagacatg cgccagggtc acccacaacg gtatcttgtc attgcggtct tcccaggact
     3121 ggggaacggc gtcgtcaaac tcgtcgaact ccctctgctg ggaagccagg cgggcgaact
     3181 ccctgcggaa aaggagaaaa tacgccaaat tggaaatgac gatggcggaa acggccttcc
     3241 atccgaaatg ctggatcatg tgagccagcc cccagtccca cttttccgcc accatcacta
     3301 cgggtggagc tgcaaagtgc gtcagggtgc ccccgatgg
//
LOCUS       DAOMSU010000238         3335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2882_length_3335_cov_2.403049, whole genome shotgun sequence.
ACCESSION   DAOMSU010000238 DAOMSU010000000
VERSION     DAOMSU010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2882_length_3335_cov_2.403049"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>281
                     /locus_tag="PK077_08875"
     CDS             <1..>281
                     /locus_tag="PK077_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRN24450.1"
                     /translation="WYGSDKIAAKAIREGRQVVNSTSHMTYINKGYDKLPLSKSYSFE
                     PVFAGLNPEQQKNVIGLGCQVWTEWIADVEKLHRHVFPRIAAYAETGWTR"
     assembly_gap    282..292
                     /estimated_length=11
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(444..1475)
                     /locus_tag="PK077_08880"
     CDS             complement(444..1475)
                     /locus_tag="PK077_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24451.1"
                     /translation="MESLPACLSKFFPHAMLDYLFQHFYSTLAIFSFCIAILILRALG
                     KRSIFWIPVSTAPLGCITFLAVFLLLTAFIIGTFGFFTTIPIAENLAFALAWLAAMAA
                     ALTVISRLTGYYLRQEPAAGRHYPEEYGKMLSAQLRNKGILLFAIIGIISCAIDIITF
                     PFPIPELPPGSPLSAATMIPLPPEVPFEVCISSRVTTRFSGECDFDATVRKKGGGKHV
                     FPAILINPGGRSAVTIYRLWPEDKHHQGSLIFTNGIQSCLLNMDTMQMKSLNGPASNY
                     PPPSSEDLSGEDMNLRLETVGLYRGLSFVRGAEHSATTRSYILKILENMQARAECLKK
                     PQTGTLPNP"
     gene            complement(1943..3187)
                     /locus_tag="PK077_08885"
     CDS             complement(1943..3187)
                     /locus_tag="PK077_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRN24452.1"
                     /translation="MNPLKGACIIGQSGGPTAVINASALGAIQSALQSEQVTRVLGAA
                     NGIEGVLKERLFDMAREDPGELELLKYTPASALGSCRYKMSAPSVDDTDYRRLLEIFR
                     KYDVRYFFYNGGNDSMDTCNKISKFMQQSGYECRVIGIPKTIDNDLHGTDHCPGFGSA
                     AKFIATSCMEVHQDLRVYDKGRVTIVEIMGRHAGWLAGSAALATYAGAGPDLVYLPEV
                     PFRMEEFWQDVDRIYRQNGSCMVAVSEGVQYADGRFVAESGDRDVFGHTQLGGLGAML
                     AESVKRQTGAKVRSIELSLLQRCASHVASKTDIDEAYMAGKAAVEAAVSGQTDKMVAF
                     ERITENGIYSCKTKLIGLGEVANVEKLVPREWINARGNGVEQPFIDYVLPLIQGETAM
                     QKEYSLPRFAKLRKVPAKPKQR"
BASE COUNT          762 a          802 c          962 g          798 t
ORIGIN      
        1 tggtatgggt ccgataaaat tgccgccaag gcgatcaggg aaggccgcca ggtggtgaat
       61 tccacttccc acatgactta cattaacaaa gggtatgaca agctccctct gtccaagtcc
      121 tattcttttg agcccgtttt tgcgggattg aatccggagc agcagaagaa tgtcatcggg
      181 ctgggttgcc aggtctggac ggaatggatt gcggacgtgg agaaactgca ccgtcatgta
      241 tttccccgta ttgccgctta tgcggagacg gggtggaccc gnnnnnnnnn nnaaattttc
      301 aggatttccg gaggcgtttg acggggtatg aaaaaattct ggatgccctg ggcatccggc
      361 acggggaaaa ggaatagttg aaaattcgcc gccggggatg aagcattctg gctgagtcaa
      421 cagtaagccc gcttgttact tggttaagga ttcggaagag ttcctgtctg tggcttctta
      481 aggcattccg cccgcgcctg catgttttcc agaattttca gaatataaga acgcgttgta
      541 gccgagtgtt ccgctccgcg tacgaaggaa agccccctgt acagtcctac cgtttccagg
      601 cggagattca tatcttctcc ggacaggtct tcactggagg gaggaggata attggaagcg
      661 gggccgttca gggacttcat ctgcatggta tccatattaa gcaggcagct ttgtattccg
      721 ttagtgaaaa tcaggctgcc ctgatgatgc ttgtcctccg gccacagcct gtaaatggta
      781 acggcgcttc tccctccggg attgatcaaa atggcgggaa aaacgtgttt gccgccaccc
      841 tttttccgga cggtcgcgtc aaagtcgcat tctccggaaa acctggtcgt aacacgggag
      901 gagatgcata cttcaaaagg tacttccggc ggcaggggaa tcatggtggc ggcagatagg
      961 ggtgaccccg gagggagttc cggaattggg aaggggaacg taatgatatc aatggcgcag
     1021 ctgatgatgc cgatgatggc gaatagcagg atgcctttgt tccggagctg ggctgacagc
     1081 atttttccat attcctccgg ataatgcctg cctgcggctg gttcctgccg caggtaatag
     1141 ccggtcaggc gtgaaataac ggtcagagcg gcagccatgg ctgccagcca tgccagggca
     1201 aaagccagat tttccgctat gggaattgtg gtgaaaaagc cgaatgttcc aataatgaac
     1261 gccgtcagca ggagaaagac agccaggaag gttatgcagc ctaaaggggc cgtggagacg
     1321 ggaatccaga aaatgctgcg tttgccgagg gctctcagaa tcagaatggc aatgcaaaaa
     1381 gagaagatgg ccagcgttga ataaaaatgt tgaaacagat aatcgagcat ggcatgcggg
     1441 aaaaacttgg ataggcaagc ggggagcgat tccatgccga tttcctgaaa agtatccgga
     1501 aacaagcagt tttgcgccgc gaaacgatag aagctgaatg ttgaactcct ttattattct
     1561 agcctttttc catccttgcc tgcaagattt tttgtttacc tgtttctttc tatttgtttt
     1621 tgatatgttt aaaaagcatt gtcccataaa caaatatggg gaaaaaccga ctccattttc
     1681 atttggtgaa aaagatgcgg tttcctataa gcggaggatt ccgaaatggt aaaactgtgt
     1741 gttatggata aggggaaaag agtattcctg ccatggaaat gttttttccc gtttatgaat
     1801 gaaaccgctg cgccggcttt cccttcatcc ttttcaagag accgggcggg gagaagagcc
     1861 ccggccggat tttacctgca agagcgtggg gcaggcagtt ccggacgggt tgatgaggaa
     1921 aaacgcttcc ctgaagaaac tgctaccgct gtttcggctt tgccggcact ttcctgagtt
     1981 tcgcgaagcg gggcagggaa tattcctttt gcatggccgt ttctccctgg atcaggggaa
     2041 gaacgtaatc aatgaacggc tgttccacgc cattgccgcg ggcgttgatc cattcgcggg
     2101 gaacaagttt ttccacattg gccacttctc ccagacctat caatttagtc ttgcaggaat
     2161 aaatgccgtt ctccgtgatt cgttcaaagg ctaccatttt atccgtctgg ccggaaacgg
     2221 cggcttccac ggcggctttt cccgccatat acgcttcatc gatatccgtt ttggaggcga
     2281 catgggaggc acagcgctgg aggagtgaga gttcaatgct tctgactttg gccccggttt
     2341 gccgcttgac ggattccgca agcatggctc ccaggcctcc cagctgcgta tggccgaaga
     2401 cgtccctgtc tccggattcc gcaacaaagc ggccgtctgc atattgcacg ccttccgaga
     2461 cggcgaccat gcagctgccg ttctgcctgt aaatccggtc cacatcctgc cagaattcct
     2521 ccatgcggaa gggaacttcc ggaaggtaaa ccaagtccgg acctgctccc gcatacgtgg
     2581 ccagggctgc ggatccggcc agccagccgg cgtggcggcc catgatttcc acaatggtga
     2641 cgcgtccctt gtcgtacacg cggaggtcct ggtgaacttc catgcaggag gtagcgataa
     2701 atttggcggc ggagccgaag ccggggcagt ggtccgtgcc gtgaaggtca ttgtcaatgg
     2761 ttttggggat tccgatgacg cggcattcgt aaccggactg ctgcatgaat ttggatatct
     2821 tgttgcaggt atccatggaa tcgttgcccc cgttgtagaa aaagtagcgt acgtcgtatt
     2881 ttcggaatat ttccagcaga cggcggtaat ccgtatcatc cacggatggg gcggacatct
     2941 tgtaacggca ggaacccagg gccgaggccg gagtatattt gagcaattcc aattctccgg
     3001 ggtcttcccg agccatatcg aacaggcgtt ctttgagcac tccctcaatc ccgttggcgg
     3061 ctcccagcac gcgcgtgacc tgttcgcttt gcagggcgga ctgaatggct cccagtgcgc
     3121 tggcgttgat gacggcggtg gggccaccgg actggccgat gatgcatgct cctttcaatg
     3181 ggttcatggg caataatgga tgagagagtt gtgttgcgcc ccctttcaac ggcgttggag
     3241 gcaggaaatg aaatgatagt caaacctatc cgcagcccgg agggatgtca aacattattg
     3301 gttttccgga tggggctggc ggggctggag aaagt
//
LOCUS       DAOMSU010000239         3253 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2981_length_3253_cov_2.293934, whole genome shotgun sequence.
ACCESSION   DAOMSU010000239 DAOMSU010000000
VERSION     DAOMSU010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2981_length_3253_cov_2.293934"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            479..742
                     /locus_tag="PK077_08890"
     CDS             479..742
                     /locus_tag="PK077_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HRN24453.1"
                     /translation="MNSENLHRRLKKIIGQVQAIDRMIDEDVPCEDVLAQLNAAKSAL
                     HKVGQVVLEGHISHCVRDGLEHGDPEETIARFTKAVERFANMI"
     gene            977..2752
                     /locus_tag="PK077_08895"
     CDS             977..2752
                     /locus_tag="PK077_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRN24454.1"
                     /translation="MEPLPFEVSWIAIVLCGVPVVLEAVLGLATAWDIKAGVLVSIAL
                     IASVIIGEDFAAGEVAFIMQLGELLEELTVARARKGIERLVRLTPATARKVEGTREEV
                     IPAEEVRIGDILRVLPGETIPADGVILSGHTSVNQSVMTGEPLPIDKETGDEVSSGTV
                     NQFGTFDMKAARTGKDSSIQRMVRLVQSADAGKAKIVRLADRWATWIVAIALVSAGGT
                     WMATGELIRAVTILVVFCPCALVLATPTAVMAAIGNATRHGFLVREGDALERLSCVSH
                     LTFDKTGTLTCGAPRVAAVRSFLPELPEQELYRYAACAELRSEHPLGKAIVRCYRKDS
                     VRELPQPEQFRMIPGRGVRAVAQGKELLAGNLELFRENGVELSDEARRAAERYQEEGC
                     SVIFIGMDRQAAGLIALSDTLRENAVATIREVRDAGVVPVLLTGDHGNAAARVAGQLG
                     IDLVCAECLPEDKFNWIDACQKERNRVCMVGDGINDALALKTSHAGIAMGGIGSDIAV
                     DAADIVLVNDDIRELPHLLRLSRRMMGTIKCNLTFSMALNFIAIILAAGGILNPVAGA
                     LVHNAGSVVVIANSVFLLKWRRKNA"
     regulatory      2888..3007
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3073..>3253
                     /locus_tag="PK077_08900"
     CDS             3073..>3253
                     /locus_tag="PK077_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24455.1"
                     /translation="MNDTSNLSYPGSRRIYVPGRLYPDVRVPMREIILGDTLLPDGTA
                     HPNDPVRVYDCSGPWG"
BASE COUNT          704 a          810 c         1012 g          727 t
ORIGIN      
        1 gtttatgaag aaagcatgaa ggaatgcatg gagcggaaaa cagggattcc tcaatggtac
       61 gttattgtgg acgggcagcg gaataccatt gcgggagccg gagtaattga aaacgatttt
      121 catgaccgga aggacttaac tcccaattta tgtgatcttt ttgtagagga aaggaaacgc
      181 gtcaggggaa tagccggggt ggtgccggat tttgtgagga aggatttggc ctctctcgga
      241 tatcccaggc tcccaaccac actccgcgcg gaaagaagag caaggaatga agaaagttcc
      301 ccggcaaaag gaaaagaggg gaaaataatg aaaaaccgtc cggtgttttc gttggaagat
      361 gcacggagcc gcctgccggg ggaggactct gttcttgaca gcatttcctg tctgcctgat
      421 aacttgataa tacgagtacc cctcagcagc atcaggaaac gccgccatga aacaatgcat
      481 gaattccgaa aatttgcatc gccgcctcaa gaagattatc ggacaggtgc aggcaatcga
      541 caggatgatt gacgaagacg ttccctgtga agatgttctt gcgcagctca atgccgccaa
      601 gtccgccttg cataaggtgg ggcaggtcgt actggagggg cacatcagcc attgtgtccg
      661 ggacgggctg gaacacggag acccggaaga gacgatcgcg cgcttcacca aggcggttga
      721 gcggtttgcc aacatgattt gaatgggaca atgggttaat gcctttttcc ggcaggttcg
      781 ggggtggctc tcatattttt tttcttgaaa acctataccc ctataggtat ataaatgccc
      841 gtacctgtat gggtataaaa agaaaaatca tcatatgctg gaccgattgg aaaaattgct
      901 ggaatgggga gggacaaggc ggaaagtcgc actgttgtcc gtttccggat tagctctgtt
      961 aatgagcatg cttggcatgg aacctttgcc ctttgaagtt tcatggatag ccattgtgct
     1021 gtgcggcgtt ccagttgtgc tggaagccgt tcttggcctg gcgacggcct gggatattaa
     1081 ggcgggagtg cttgtttcca tcgcgttgat cgcctccgtc atcattgggg aggacttcgc
     1141 cgcaggggag gttgcattta tcatgcagct tggggaactc cttgaggagc tgaccgtggc
     1201 ccgggcccgg aagggtattg aaaggctggt ccgtttgacc ccggcgaccg cccggaaagt
     1261 ggaaggaacc agggaggagg ttattccggc ggaagaggtt cgcatcgggg atattctccg
     1321 cgtattgccc ggagagacga ttccggcgga cggcgttatt ctttccgggc acacttccgt
     1381 caaccagtcc gtaatgacgg gggaacctct tccgatagac aaggaaaccg gggatgaggt
     1441 ttccagcggg acggtcaacc agttcggaac ctttgacatg aaggcggcca ggaccgggaa
     1501 ggacagttcc atccagcgca tggtgcgcct cgtccaatcc gcagatgcgg ggaaggctaa
     1561 aatcgtgagg ctggcggacc gctgggccac ctggatcgtt gccattgccc tggtttccgc
     1621 aggggggacc tggatggcca cgggcgaatt gattcgcgcc gttaccattc tggtggtatt
     1681 ctgcccctgt gcgcttgtgc tggcaactcc cacggcggtc atggccgcca tcgggaacgc
     1741 caccaggcat ggctttctgg tgcgggaagg ggatgccctg gaacgcctgt cctgcgtgtc
     1801 ccatctcact ttcgacaaga cgggcacgct tacctgcggt gctccgcgcg tggcggccgt
     1861 gcgcagtttc ctgccggagc tcccggaaca ggaactttac agatatgccg cctgtgcgga
     1921 actgcgttct gaacatcctc tgggcaaggc gattgtccgc tgttaccgga aagattcagt
     1981 gcgggaactt ccccaaccgg aacaattccg catgattccc ggcaggggag tacgggctgt
     2041 ggcgcaggga aaggaacttc tggccggcaa cctggaattg ttcagggaaa acggggtgga
     2101 gctgtccgat gaggccagac gtgcggcgga acggtatcag gaggaggggt gctccgtgat
     2161 tttcatagga atggaccggc aggccgcagg gttgatcgcc ctgtcggata ccttgcggga
     2221 gaatgccgta gctaccatcc gggaagtacg ggatgccgga gtggttcccg tattgctgac
     2281 cggggaccat gggaatgccg ccgcgcgtgt cgccggacaa ctggggattg acctggtttg
     2341 cgcggaatgc ctgccggagg acaaattcaa ctggattgac gcgtgccaga aagagcggaa
     2401 ccgtgtgtgc atggttgggg acggaatcaa tgatgccctt gctttgaaaa cctctcatgc
     2461 gggaattgcc atgggcggta taggaagtga cattgccgtg gatgccgcag atattgtcct
     2521 ggtcaatgac gatatccggg aactgcccca tctgctccgt ctgtcaaggc gcatgatggg
     2581 gacaatcaaa tgcaatctga cattctccat ggccctgaac ttcatcgcca tcatcctggc
     2641 ggcgggcggt atcctgaatc ccgtggctgg cgcgctggtt cacaatgcgg gctccgtcgt
     2701 cgtgatcgcc aactccgttt ttctgctgaa atggaggagg aaaaacgcgt gaatccgcct
     2761 ggagggtaga gaaactttcc cgtgttgatt gccgggaggg aacggagtcc gtctttttgg
     2821 cgctccggtt tcaccgcgcc gggctggaaa acgcttggat gaaaagaaag tccgtgttat
     2881 tctttttcca gttcggcagg ggtgcccaaa taaaggctga gattgcagga acggcatgct
     2941 gttcccgcag aacccttcaa acctgatgcg aataattccg ccgcagggag ttcgattgaa
     3001 cgagcttgtc ctgtttttcg gggaggtttc gtttccgctg ttcccctgcc ggaacagaac
     3061 agcattcatc ccatgaacga tacctccaat ttgtcttatc ccggttcccg ccgcatttat
     3121 gttccgggcc gtctgtaccc ggatgttcgg gttcccatga gggaaatcat tttgggcgat
     3181 accttgttgc ccgacggcac cgcccaccct aatgacccgg tgcgcgtgta cgattgctcc
     3241 ggcccctggg gag
//
LOCUS       DAOMSU010000240         3248 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_2987_length_3248_cov_2.412152, whole genome shotgun sequence.
ACCESSION   DAOMSU010000240 DAOMSU010000000
VERSION     DAOMSU010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2987_length_3248_cov_2.412152"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="PK077_08905"
     CDS             complement(<1..733)
                     /locus_tag="PK077_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24456.1"
                     /translation="MARLLVTSDYPDARVWTFLRNSWKNESRSLVPLDRQTALVECRL
                     TREDMPNMGVNAFTVRNGELHTASAELLIPPAGQILEPAVTPGKSQYRPGEQGNVSIQ
                     VKGPDGTPVRNGVVALAIYDKALEYIARPNITEIAKTVWGSLNETGFLSLKKMTASGT
                     QQDRGPDQPYFQSLLYRNYGPMVRKAKGIVNGFAEAVFDSGADAAASRALATGAAAPA
                     AAPVMAMAADKEGAKSDSHAAEQGNA"
     gene            complement(786..>3248)
                     /locus_tag="PK077_08910"
     CDS             complement(786..>3248)
                     /locus_tag="PK077_08910"
                     /inference="COORDINATES: protein motif:HMM:NF013957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HRN24457.1"
                     /translation="LGNLTSLEELPGYVAREEPPPFRNVATVPVKVNAKTGKPEVVFY
                     HASSSWETAKNDGERMRWLLDAAIQANPELANQVNYFTAFWCRRLFSYANTAPDQEFV
                     YGPGNAGMVAGINPAKLKTNQTVVKTDRTGNGKFLLATLPPDYDFIRIASAVRITPEP
                     DDYINAAHLAADEFLARNQRPSAAQLLGKALQNWNAQSWDKKDRKEFLNVADNLKKRI
                     ASITEPNGTFDTDKRTLLAGEPVTVSFSYRNASRACVAVHPVDMKRWQEERMDKIQTS
                     KTLEKAYRNGYSNLANLLSNLLHDPSCARYLGEEIKGDEITLTPGNRYLNHIAHIPVP
                     TRKPGWYLLTVTLENGYRFHRFLTLSDMVLVRRSVPEGNLWFLADAGTGMPVEGGSLR
                     LLRYRQDKTLQKRQVKGITDKDGAMIETIPHRHSSAPPYNMFLGIASKGDSYVLAGIS
                     DYGWESGNYLMDTANKASDSYSCFFLESQPVYRPGQTARFKGVLFRPDIANPGTKDCA
                     GKKLTLTIASPTGDESIFPPKTVTTDSTGSFELELLIPADAPLGQYGAKLKLHDSADP
                     DFRLYAPLFRMEEYKKPEFSVRMEAPAKPVRLGQPIPVAIQADYYAGGPVSEGKATIT
                     ITRTLGADVWTPYWKWSWLYDRSFSPYYIPFFPDAPLTVLEIRRIIHGAGCPGFPLQQ
                     HHLPRFRQRDGRLPAGSCRFRTGHCHVPSVQYLHHPEPRLCPRRNAGPGIHYRGHGGW
                     RQNCPCKGNLRPAAYLCGRQTGNAGNLGHRHRKRWGSLPVLPDRGIRPVCPFHNPGGR
                     ARQQGRRDLPVPFLRQGQTESF"
BASE COUNT          586 a          874 c         1028 g          760 t
ORIGIN      
        1 ccgcatttcc ctgttcggct gcatgggaat cactttttgc gccttccttg tccgctgcca
       61 tagccatgac tggagcggcg gcgggggctg ccgctcctgt agccaatgcg cggcttgcgg
      121 cagcatctgc accggaatcg aacactgcct ccgcaaagcc gttaacgatc cctttggcct
      181 ttctcaccat agggccgtaa tttctgtaga gcagggattg gaaatagggc tggtccggac
      241 cgcggtcctg ctgcgttccg gaagccgtca tttttttcag gctgaggaag ccggtttcgt
      301 tcaggctgcc ccatacggtt ttggcgattt ccgtaatatt ggggcgcgcg atgtattcca
      361 gagccttgtc ataaatggcc agggcgacga cgccgtttct gacgggggtg ccgtccggcc
      421 ccttgacctg aatgctgacg ttgccttgtt caccgggccg gtattgggat ttgcccggag
      481 tgacggcagg ttccagaatc tgcccggcgg gcggaatcag cagttcggcg gaagccgtat
      541 gaagttctcc gtttctgaca gtgaaggcgt tgacgcccat attgggcatg tcttcccggg
      601 tcaggcggca ttccacaagg gctgtctgcc ggtccagggg caccaggctg cgggattcgt
      661 ttttccagga gtttcgcagg aaggtccaga cgcgggcgtc cggataatca gaggtgacga
      721 gcagcctggc catatctccc ggagcgtatt cctttttgtc cggagtgatg gaaaggggat
      781 tgattttaaa aggattctgt ttgcccttgc cgtaggaaag gaactggaag gtctcttcta
      841 ccctgttgcc gtgcccgtcc tccagggttg tggaaagggc atacaggccg gattccccgg
      901 tctggaagga cagggaggct tccccatctt ttccggtggc gatgtcccag gtttccagcg
      961 tttcccgttt gccgtccgca cagatatgcc gcaggacgca ggttcccctt gcatgggcaa
     1021 ttttggcgcc atccgccgtg gccgcggtaa tggatgcctg gaccggcgtt ccggcggggg
     1081 catagccgcg gttcagggtg gtgaagatat tgaacggacg gaacgtggca atgacctgtc
     1141 cggaagcggc aacttcccgc agggaggcgt ccgtcacgct gacggaaacg cggtaggtga
     1201 tgttgctgga ggggaaatcc cgggcatcct gcgccgtgga taattctacg gatttccagg
     1261 acggtcagag gcgcatccgg gaaaaaggga atgtagtacg ggctgaaaga cctgtcgtac
     1321 agccagctcc atttccagta aggcgtccag acatccgctc ccagcgtccg ggtaatcgtg
     1381 atggttgcct ttccctcgct tacggggccg cccgcgtaat aatccgcctg aatggcgacg
     1441 ggaatgggct gacccagccg gactggtttg gcgggggctt ccattctgac ggagaattcc
     1501 ggctttttat actcttccat ccggaaaagg ggggcataca gccggaaatc cgggtctgcg
     1561 gagtcatgca gtttcagctt ggccccatac tggcccagcg gggcgtccgc aggaataagc
     1621 agttccagtt caaagctgcc tgtggaatcc gtcgtgacgg tctttggagg gaaaatgctc
     1681 tcatcccctg ttggagaggc aatggtcagc gtcagttttt ttccggcgca gtcttttgtt
     1741 ccgggattcg cgatgtccgg gcggaagagg acgcctttga aacgggccgt ctgtccgggg
     1801 cggtacacgg gctggctttc caggaaaaag caggaatagg agtcggaggc cttgttggcc
     1861 gtatccatga ggtaattgcc ggattcccag ccgtagtcag atattcccgc cagcacataa
     1921 ctgtccccct tggaagcgat gcccaggaac atgttgtagg gaggggcgct ggaatggcgg
     1981 tgagggattg tttctatcat ggctccgtcc ttgtccgtga ttccctttac ctggcgtttt
     2041 tggagcgttt tatcctgccg gtagcggagg agccgcaggc tgcctccttc cacgggcatt
     2101 cccgttccgg cgtccgccag gaaccagaga ttgccttccg gaacggaacg gcgtaccagc
     2161 accatgtctg acagagtaag aaagcgatgg aaccggtagc cgttttccag tgtgacggtg
     2221 agcaggtacc agccgggttt gcgggtgggt acgggaatgt gagcgatgtg gttcaggtac
     2281 cggttcccgg gagtcagggt tatctcatcc cccttgattt cctcccccag atagcgggca
     2341 caggatgggt catgcagcaa attggagagc aggtttgcca gattggaata cccgtttctg
     2401 taagcttttt ccagcgtttt ggaggtctgg atcttgtcca tgcgctcctc ctgccaccgc
     2461 ttcatatcta cggggtggac cgctacgcaa gcccgggagg cattgcggta ggagaaggag
     2521 acggtcaccg gttctcctgc cagcagcgtc cttttatccg tatcaaatgt cccgttgggt
     2581 tccgtgatgg aggcgatgcg tttcttcagg ttgtccgcca cgttcagaaa ttccttcctg
     2641 tctttcttgt cccaggattg cgcgttccag ttctgaaggg cttttcctaa taactgcgcg
     2701 gcggaaggcc tctggttgcg ggccaggaac tcgtccgctg ccagatgggc ggcgttgatg
     2761 taatcatccg gctccggagt tatccggacg gcggaagcaa tccggatgaa atcgtaatcc
     2821 gggggaaggg tggcgagcag gaatttgccg ttgcccgtcc ggtcggtttt aacgacggtc
     2881 tggttcgttt ttaatttagc cgggttgatg cccgccacca tgcccgcatt gccgggaccg
     2941 tacacgaatt cctgatccgg cgccgtattg gcataagaga acagccggcg gcaccagaat
     3001 gcggtgaaat aattgacctg gttggccagt tccggattcg cctgaatggc ggcgtccagc
     3061 agccagcgca tgcgttctcc gtcgttttta gccgtctccc aggaggaaga ggcatggtaa
     3121 aagacgactt ccggcttccc cgttttggca ttgaccttga caggcacggt ggcgacgttc
     3181 cggaacgggg ggggttcctc cctggcgacg taccccggca gttcctccag gctggtgagg
     3241 ttccccag
//
LOCUS       DAOMSU010000241         3198 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3051_length_3198_cov_2.586382, whole genome shotgun sequence.
ACCESSION   DAOMSU010000241 DAOMSU010000000
VERSION     DAOMSU010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3051_length_3198_cov_2.586382"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="PK077_08915"
     CDS             <1..327
                     /locus_tag="PK077_08915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24458.1"
                     /translation="FRENDFLVFSKTPSRKPVYPIVKFIRSPYPERGNYQLVYWRIQK
                     KPSGPSRKNDLQAFSNMANEHINQCRAFIFLIENETPCGEVFDYLNAIDGRDTLVGVT
                     FSRENE"
     gene            428..1660
                     /locus_tag="PK077_08920"
     CDS             428..1660
                     /locus_tag="PK077_08920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN24459.1"
                     /translation="MTRTRDELAAETAPSVKAPLWTRDYILACCANLMTGLAFYELVP
                     VLPLYLTGQLQVSSGWLGWIMSIYVLAAIISRPWFAHRVDTGDRKKIYITVYIMLALS
                     FSGYAAAATALAFFLTRFIQGLIWGGMTTSGPTMAVDIIPPARRGEGLGFFGMTMTLG
                     MCLGPVIGLQVYQQYGFYVITWSSLLLCLAGAGIASLIRVPGSPVQEPVYETPKKVLD
                     RLVLRVGIPLAVNVMIATFSYGVVAVYSALYGEMHGFKYAGLFYALMGLGMLVSRFMV
                     GRQIDRGRVAELSVISLSILTVSFGALALAPLEWIYYASAILIGFGFGIFIPTFQTMK
                     LNMADRGHRGAVNSTFFTAFDIGVGTGMFLGGKIYAYLNLNWAFGTGALLNLLAIVYF
                     YRISLDHYRKNKLGVDSD"
     gene            complement(1672..2157)
                     /locus_tag="PK077_08925"
     CDS             complement(1672..2157)
                     /locus_tag="PK077_08925"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HRN24460.1"
                     /translation="MDIDFGIVRTSALEAEGLESRVICSMDYALYVPAALAGKKAQGK
                     KEDFLRLLEMFPLATLGSGSGFHALLKRNCAEFGIRLRVHCETQSFPFAARMLKSGAF
                     MAILPCMAEKELGEGFVKVTHPALDGLSRSISLAWNPRLLRVRPSAKRVIDVFSIPEQ
                     E"
     gene            complement(2148..2573)
                     /locus_tag="PK077_08930"
     CDS             complement(2148..2573)
                     /locus_tag="PK077_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HRN24461.1"
                     /translation="MFEHLFAERGLSLDRLKTLIEVAKAGSIAAAARGDSARQSLYSR
                     QIKELEEFFGVELASRRGKVLALTGAGWELVRLASESLCLLDDFKSRSRNLPYRFTIG
                     AGDSLHAWVVAPVLASIRGFSRWRTSATVRFPLSSRIWI"
     gene            2704..>3198
                     /locus_tag="PK077_08935"
     CDS             2704..>3198
                     /locus_tag="PK077_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRN24462.1"
                     /translation="MITIDGKYTEARVFTDELEDLARTQIRDVCNHPTFEGSRVRIMP
                     DVHAGAGCVIGFTAELKTDKVIPNLIGVDIGCGVLTARLSGLPDFSSFDARLRERVPS
                     GMHARPSVHQALLGDPELDEEISRICMDVLRTDKDRHRRSVGSLGGGNHFIEIAQGKE
                     HAFLC"
BASE COUNT          696 a          970 c          828 g          704 t
ORIGIN      
        1 ttccgtgaaa atgattttct tgttttcagc aaaacacctt cccggaaacc cgtttatccc
       61 atcgtgaaat tcatccggtc cccttacccg gaaagaggga actaccagct tgtttactgg
      121 cgtatccaga aaaaaccatc cggtccatcc cggaaaaacg accttcaggc tttctccaat
      181 atggcaaatg aacatatcaa ccagtgcaga gcattcattt tcctcattga gaacgaaaca
      241 ccctgcggag aagtattcga ctatctgaat gccattgacg ggagagacac gctggtgggc
      301 gtaacctttt cccgtgaaaa tgaataacgt ctcccaacaa ggattcatca gggctttcct
      361 ccccccgctc atcatcatga cacgtcaggc cgtttgaaac ttttcccaac ggctgtacaa
      421 cccctgcatg acccggacca gagatgaatt ggcggcggaa accgcaccat ccgtcaaagc
      481 gcctctctgg acgcgagact acatcctggc ctgttgcgcc aacttaatga cggggctggc
      541 cttctatgaa ctggtgcccg ttctcccgct gtatctgacg gggcaattgc aggtctcctc
      601 cggctggctg ggctggatta tgtccattta tgttctggcc gccattatct cccgcccctg
      661 gttcgcacac cgggtggata ccggagaccg caagaagatt tacatcacgg tttacattat
      721 gctggccctg tccttttcag ggtatgctgc ggccgccacc gccctggcgt ttttcctgac
      781 gcgcttcata cagggcctga tctggggagg catgaccacc tccggcccga caatggcagt
      841 agatatcatc ccccctgccc gccgcgggga aggcctgggc ttcttcggca tgacaatgac
      901 gctgggcatg tgcctgggcc ccgtcatcgg ccttcaggta taccagcagt acggctttta
      961 cgtgattacc tggagttccc tgctcctgtg cctggccggg gcgggcattg catctctcat
     1021 ccgcgttccc gggagccctg ttcaggagcc cgtgtatgaa acgcctaaaa aggtactgga
     1081 ccgtctggtg ctgcgcgtgg gcattccgct ggcggtgaac gtgatgatcg ccaccttctc
     1141 ctatggcgtg gtggccgtgt attccgccct gtacggagaa atgcacgggt tcaaatacgc
     1201 cgggctgttt tacgcgctga tggggctggg catgctggtc tccaggttca tggtaggcag
     1261 gcaaatcgac cgcggacgcg tggcggagct ttccgtcatc tccctgagta tcctgaccgt
     1321 cagcttcggc gcgctggcgc tggcgccgct ggaatggatt tattacgctt ccgccatcct
     1381 catcggcttc ggtttcggca ttttcatccc cacgttccag actatgaagc tgaacatggc
     1441 ggaccgcggc caccgtggag ccgtcaactc cacatttttc accgcttttg acatcggggt
     1501 gggaacaggc atgttcctgg gaggcaaaat ttacgcgtac ctgaacctga actgggcctt
     1561 cggaacggga gccctgctga atttgctggc gatcgtatat ttttaccgca tctccctgga
     1621 ccactaccgt aaaaataaat tgggcgtgga ttctgattga ttttccttcc atcactcctg
     1681 ttccgggatg gaaaaaacat caattacccg tttggctgaa ggcctcacgc gcagcaaccg
     1741 cggattccag gccagggaaa tactgcggga cagcccgtcc agcgccggat gagtaacctt
     1801 gacaaatcct tcccccagtt ctttttccgc catgcagggc aggattgcca tgaacgctcc
     1861 gctcttcaac atgcgggccg caaaaggaaa agattgcgtt tcacagtgca cccgcagcct
     1921 gatgccgaac tcagcgcaat tccttttcag caacgcatga aacccggaac cgctccccag
     1981 cgttgccagg gggaacattt ccagcaggcg gagaaaatcc tccttcttcc cctgcgcctt
     2041 tttcccggcc agggctgcag gaacgtacag cgcataatcc atggagcaaa tgacccggct
     2101 ttccaaccct tccgcctcca gcgcgctggt acgcacaata ccgaagtcta tatccatatt
     2161 ctggagctta gagggaatct cactgttgcg gaggttctcc agcgcgaaaa gccacggata
     2221 ctcgccagca cgggagccac cacccaagca tgcaggctgt ccccggctcc gatagtgaac
     2281 cggtagggca gattccggct ccggctttta aaatcatcca gcaggcacag gctttcactg
     2341 gccagcctca ccagctccca cccggctccg gtaagagcca gcactttccc gcggcggctc
     2401 gccagttcca caccgaagaa ttcctccaat tccttaatct gccgggaata caggctctgc
     2461 cgcgcactat ccccccgggc ggcagcggca atgctcccgg ccttggccac ttctatcaag
     2521 gttttcaatc ggtccaggga cagtccccgt tctgcgaaaa gatgctcaaa cataacttta
     2581 tagttatata atatgaatac ttatataatg caagagtcaa caaatgcgtt atggtgacaa
     2641 ggtcacagga cgcgaaggcg ctttccgcat atctccgcgc ccctcaacca aaagaataaa
     2701 accatgatca ccattgacgg aaaatacacg gaagcccgcg tgttcacgga tgaactggaa
     2761 gacctggccc gcacccagat cagggacgtc tgcaaccacc ccacctttga aggaagccgg
     2821 gtgcgcatca tgccggacgt gcacgccgga gccggctgcg tcatcgggtt cacggcggaa
     2881 ttgaaaacgg ataaggtaat tcccaacctg atcggcgtgg atatcggctg cggagtgctt
     2941 accgccagac tgtccggact gccggatttt tccagctttg acgcacgcct gcgcgagcgc
     3001 gttccgtccg ggatgcatgc ccgtccctcc gttcatcaag ccttgctggg cgacccggaa
     3061 ctggatgaag aaatctcccg catctgcatg gacgtgctga gaacggacaa agacaggcac
     3121 cgccgctccg tagggtctct gggaggaggc aaccacttca ttgaaatagc gcaagggaaa
     3181 gagcacgctt tcctgtgc
//
LOCUS       DAOMSU010000242         3196 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3056_length_3196_cov_2.403056, whole genome shotgun sequence.
ACCESSION   DAOMSU010000242 DAOMSU010000000
VERSION     DAOMSU010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3056_length_3196_cov_2.403056"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..605
                     /locus_tag="PK077_08940"
     CDS             <1..605
                     /locus_tag="PK077_08940"
                     /inference="COORDINATES: protein motif:HMM:NF022019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRN24463.1"
                     /translation="ETTYNRRKERNNRLRVIFENGTEADLLMRSLFCALYDGGRLVSE
                     PDETELKLEGPVNEDDCLTGCVYVLKSASRLPEVRAIPHLYKIGVSSVPLEERIAHAR
                     HEATYLMADVIPVRKWDCYNLDARKAENLLHHFFAAACLDLEIRDGSGRLCRPREWFS
                     VPLGIIEQVIPLLLDGSIIHYRYDPESMTLEKRAGDGKGR"
     gene            complement(713..>3196)
                     /locus_tag="PK077_08945"
     CDS             complement(713..>3196)
                     /locus_tag="PK077_08945"
                     /inference="COORDINATES: protein motif:HMM:NF019298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HRN24464.1"
                     /translation="ADKEGAKSDSHAAEQGNADVQENGSPHIQLRTHFADCIKWCGTL
                     KTDEEGNVAVPVEMPDNLTTWKASAWVITPGLQVGQASAEFLTTKDFMVSMQAPRFFV
                     EKDVVMLSALIRNRTDKNIRARVSISLKNGCLELLPADAPAVKGLSADSDNSAAREVD
                     VPAQGQTVVNWWTSAVREGTAAVAMEAASGPTGDAMQMNFPVLVHGMKQLHAESAAVL
                     SGEQEKTLSISLPQQRRREESELVVKVSPSIALSMVEALPYLAEYPYGCVEQTLNRFL
                     PALVVMDTLKQLGLNPGAALTSHRNLNPRDIRDKAFHDSVMKKLERNPVYDEAALKKM
                     AARGISSLREKQLSNGSWGWFGGASEGDPVMTAHVAHGLKIASNTVNVPEGMLSKAVR
                     WLKNYQERQTALLEKGDEYRKLEQLPDGPKKKEAIRKLGDYRLMASATDALVYSVLAE
                     CGVKNLPMERYLFRDRLELPVISQIQLAEILLDAHRMDDFKKVMPIISQFLQQDDSLQ
                     TAWLRLPNEGYWWRWYGSGTATQAAYLKLMAKSAPGNPVTARLAKWLLDNRANGSYWD
                     STKDTADCLEALSAYLLQTREGMEDMEAEILYDGVPVKTIASTKETLFTFDNAFRMSG
                     KDLTDGNHVITIRRKKGRGNIYANAALSYFSLEDPIPAAGNAVTVERSYYRIRKETVK
                     DGSVKDTQTDAGELVSQGRDLTRRTLLKNGDVIASGDIIEVVMTVKTKNDVEYLMLLD
                     PKPAGCESRETASGYARLGTVSGYREIGDEEIRLFLSSLPMGQYQISHRLRAERPGRF
                     SALPAVIEAMYAPELRGNSREHKIGISMP"
BASE COUNT          571 a          881 c          996 g          748 t
ORIGIN      
        1 gagagactac gtataaccgc cgcaaagaac ggaataaccg tttgcgcgtg atttttgaaa
       61 atggaacgga ggccgatctg ctgatgcgct ccctgttttg tgccttgtat gacggcggcc
      121 gcctggtcag cgagccggat gaaacggaat tgaagctgga aggccccgtg aatgaagatg
      181 actgccttac cggctgcgtg tatgtgctga aatccgccag caggctcccg gaagtgcggg
      241 ccattcctca tctgtacaaa ataggggtaa gttccgtgcc tctggaagaa cgtattgccc
      301 atgctcgcca tgaggcgacg tatctgatgg cggatgtcat tcccgtgagg aagtgggatt
      361 gttacaatct ggatgcccgc aaggcggaaa acctgcttca ccatttcttt gccgccgcct
      421 gcctggattt ggaaatcagg gatgggagcg ggcggctgtg tcgtccgcgg gaatggtttt
      481 ccgttcctct ggggattatt gaacaggtga ttcctctgtt gctggacggc agcattattc
      541 attaccggta tgatccggag tccatgacgt tggaaaaacg ggcgggagac ggaaaaggaa
      601 ggtgaggggg aaggcagaca ggccgctctt cacctgaccg gcctgcttcc agaggagccc
      661 ggcggaatat ccgccggggc catcatggaa tgacggatat ttccagaagg tcttatggca
      721 tggagatgcc gattttgtgt tcccggctgt tgccgcggag ttccggggcg tacatggcct
      781 caatgacggc cggaagggcg ctgaagcgtc ccggacgttc cgcgcgcagg cggtggctga
      841 tctggtattg gcccatgggc agggaggaaa ggaagaggcg tatttcctca tcccctattt
      901 ccctgtagcc ggaaacggtt cccagccggg cgtagccgct ggcggtttcc cgggattcac
      961 agcctgcggg cttggggtcc aggagcatga ggtattccac gtcgttcttc gttttcacgg
     1021 tcatcaccac ttcaatgatg tcgccgctgg caatgacgtc cccgtttttc aacagcgtgc
     1081 gccgtgtcag gtctctgccc tgggagacga gctcccctgc atccgtctgg gtgtccttca
     1141 cggaaccgtc tttcaccgtt tccttccgga tgcggtagta ggagcgttcc acggtgacgg
     1201 cattccccgc ggcgggaatg ggatcttcca gggagaagta gctgagggcg gcattggcat
     1261 aaatgttccc tctgcccttt ttcctgcgga tggtgatgac atggttgccg tccgtcaggt
     1321 ctttcccgct catgcggaag gcgttgtcaa aggtgaacag ggtttctttc gtactggcga
     1381 ttgttttaac cggaacgccg tcgtacagga tttccgcttc catgtcctcc atgccttccc
     1441 tggtctggag aaggtaggcg gacaaggctt ccagacaatc cgccgtatct ttggtggagt
     1501 cccagtagga accgttggcg cggttgtcga gcagccattt tgccaggcgc gccgtgacgg
     1561 gattgccggg ggcgctcttg gccatcagtt tcaggtaggc cgcctgcgtg gccgtgccgc
     1621 tgccgtacca ccgccaccag tagccttcat tgggaaggcg cagccaggcg gtttgcaggg
     1681 agtcgtcctg ctggaggaac tgggaaatga tgggcattac cttcttgaag tcgtccatgc
     1741 ggtgggcgtc cagcaggatt tccgccagtt gaatctggct gatgacggga agttccagcc
     1801 tgtcgcggaa caggtagcgt tccatgggaa ggtttttgac gccgcattcc gccaggacgg
     1861 agtaaaccag ggcgtccgtc gcggaggcca tcaggcggta atcccccagc ttgcggatgg
     1921 cttccttctt tttcggtccg tccggcagtt gttccagttt ccggtattcg tctccttttt
     1981 caagaagagc tgtctggcgt tcctgatagt ttttcagcca gcggacggct ttggaaagca
     2041 tcccctccgg gacattgacc gtgtttgaag caatcttcag gccgtgcgcc acatgggccg
     2101 tcatgacggg gtccccttcg ctggctcctc cgaaccatcc ccaggagccg ttggagagct
     2161 gtttctcccg gagggaggag attcccctgg cggccatctt cttcagcgcg gcttcatcat
     2221 aaacggggtt gcgttccagc ttcttcatga cgctgtcatg gaaggccttg tccctaatgt
     2281 cccggggatt cagattgcga tggcttgtca gcgccgctcc gggattcagg ccgagctgct
     2341 tgagcgtgtc catgacgacg agagcgggca ggaaacggtt aagcgtttgt tccacgcagc
     2401 cgtaggggta ttccgccaga taaggcagag cttccaccat gctgagggcg atgctggggg
     2461 agaccttgac gaccagttcg ctttcctccc tgcgccgctg ctgcggcagg gaaatggaga
     2521 gcgtcttttc ctgttctccg gagagcactg cggcgctttc cgcatggagc tgcttcatgc
     2581 cgtgcaccag tacgggaaag ttcatctgca tggcgtctcc ggtaggacct gacgccgctt
     2641 ccatggcgac ggcggcggtt ccctccctga cggcggaggt ccaccagttg acgaccgtct
     2701 gcccctgggc gggaacgtcc acttcccgcg cggctgaatt gtccgaatct gcggaaagcc
     2761 ccttcacggc cggagcgtca gccgggagaa gttccaggca accgtttttc agggaaatgg
     2821 aaacgcgggc gcggatgttt ttgtccgtcc ggttgcggat cagggcgctg agcatcacga
     2881 catctttttc cacaaagaag cgcggggcct gcatgctgac catgaagtcc ttcgtggtca
     2941 ggaattccgc ggaagcctgc cccacctgca atccgggggt gatgacccag gctgaggctt
     3001 tccaggtagt cagattgtcc ggcatttcca cagggacggc aacatttcct tcttcatccg
     3061 ttttgagcgt gccgcaccac ttgatgcagt ccgcaaaatg ggtgcgcagt tgaatatgcg
     3121 gggaaccgtt ttcctgtacg tccgcatttc cctgttcggc tgcatgggaa tcactttttg
     3181 cgccttcctt gtccgc
//
LOCUS       DAOMSU010000243         3181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3076_length_3181_cov_3.048305, whole genome shotgun sequence.
ACCESSION   DAOMSU010000243 DAOMSU010000000
VERSION     DAOMSU010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3076_length_3181_cov_3.048305"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="PK077_08950"
     CDS             complement(<1..576)
                     /locus_tag="PK077_08950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24465.1"
                     /translation="MNPPHRSSSVHRSAVPGYRKWYFGAGGSPGRRGMEAAPEPDPLP
                     FEEIHEHAEITVGPPSTSADGYSAHGTFTIPEGAEGKKLYGTCSLFLGVDDWGSLEVK
                     DSGGNVVAQVDLKENPQTAGEQGGHKYHTGTGGAQLPSGTYSWEVSQTNIDYHPASGN
                     TSICNYSIDVVPTEPGGRKEPEPCPCEGDTCD"
     gene            complement(1131..1898)
                     /locus_tag="PK077_08955"
     CDS             complement(1131..1898)
                     /locus_tag="PK077_08955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HRN24466.1"
                     /translation="MAKTLNLTLKVWRQENRDAKGRIETYAAKDIPVDASFLEMLDIV
                     NEGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVW
                     IEPFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDA
                     AACIGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNC
                     TNQYECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
     gene            complement(1939..>3181)
                     /gene="sdhA"
                     /locus_tag="PK077_08960"
     CDS             complement(1939..>3181)
                     /gene="sdhA"
                     /locus_tag="PK077_08960"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
                     /protein_id="HRN24467.1"
                     /translation="VFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVKGD
                     YQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFGN
                     LAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALM
                     QGLSDGYFVIPATLPAYLTTTKPGAVTTASPEFKAAEDEVKERINKLLNVKGTKTVDS
                     FHRELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACR
                     VADFLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSL
                     PVLHKEPLTFENVHLAIRSYK"
BASE COUNT          557 a          845 c          990 g          789 t
ORIGIN      
        1 gtcgcacgtg tctccctcgc agggacacgg ttcgggttct ttcctgcccc ccggttccgt
       61 cggcaccacg tcgatgctgt agttgcagat ggaggtgttg ccgcttgccg gatggtagtc
      121 gatgttggtc tggctgactt cccagctgta ggtgccggag gggagctgcg ccccgccggt
      181 ccccgtgtgg tatttgtgcc cgccctgttc gcccgccgtc tgcgggtttt ccttcagatc
      241 cacctgcgcc accacgttgc cgccggagtc ctttacctcc aggctccccc agtcgtccac
      301 ccccaggaag agcgagcagg tgccgtagag ttttttgccc tccgctcctt ccgggatggt
      361 gaatgtcccg tgcgcactgt acccgtccgc cgacgtgctc ggcggtccca cagtgatttc
      421 agcgtgttcg tggatttctt caaatggcag cgggtcgggc tccggagctg cctccatgcc
      481 ccgcctgccg gggctgccgc cggctccaaa gtaccatttc cggtagccgg gaacggcaga
      541 acgatggacg gaggaggaac gatgaggcgg attcatcagg tattcaagag tcgggggtaa
      601 tgaaggaagc gaggggggcc gccctttcct gatcgtttta tcaatagtat gatctgaagt
      661 cggtgtcgat agaattttat ttgtatgttt ttgatttttt ataaaagatg gtcattattt
      721 tacacgaaaa gataatatta taattcttac gttcagtgca ttcccttaat agacatcaat
      781 gttgtctgtt tgttagaaaa tgttttcctc tcatgctgag cccttgacag gaggccatcc
      841 cttccgggca tcagtctaat tcctgtaatc aatatttttc tttctcggag aaagatgggg
      901 ccgtcgctgt cggacggggc ctgcttaggt ttcgtccggc atttccaagt acggtctcct
      961 ggattactgg gaaccttccg gtgtaacatt tattttttac gctgcgcgac gatgaggaag
     1021 caactgtctg atattttatt cctggttcaa ggaaacctga taatgaaaaa aactcccgac
     1081 agccgatgcc gccaggagtt tttacagagc gtcttaagaa ggaatcttct ttagaaacag
     1141 aggccttcct tggcgttggc ccgcatgaag tcgcggttga ggcgggcgat gttgtccacg
     1201 ctgatgcctt tcgggcaggc ggcttcacat tcatactggt tcgtgcagtt gccgaagcct
     1261 tccgcttcca tctgcttcat catggccagg acgcgcttgg aacgttccgg ctggccctgc
     1321 ggcagcagat tgagatgggc gatcttggcg gaggtgaaca gcatggcgga ggcgttttta
     1381 caggaggcca cgcaggcgcc gcagccgatg caggcggccg cgtcaaaggc ggcgtccgct
     1441 ttcacttttt ccacggcgat gttgttggcg ttcggcgcgg aaccggtacg cacgtcaata
     1501 tagcctccag cggcgatgat gcggtccagg gcggagcggt ccaccatcag gtcccgcagg
     1561 attgggaaag ccttcgcgcg gaagggttca atccagacgg tatcgccatc cttgaacttg
     1621 cgcatgtgaa gctggcaagt ggtcacctgg cgttccggcc cgtggggaat gccgttgatg
     1681 gtcagggagc acatgccgca gatgccttcc cggcagtcgt ggtcaaaatg gatgggttcc
     1741 ttgccttcct tgaccagacc ttcattgacg atgtccagca tttccaggaa ggaggcgtcc
     1801 accggaatgt ccttggcggc ataggtctca atgcggccct tggcgtcacg gttttcctgg
     1861 cgccagacct tgagagtgag attgagtgtt ttagccatgt tataatcttt cttttaagtg
     1921 ttaatgtatt ggctatcttt acttgtagct gcggatggca aggtgcacgt tctcgaaggt
     1981 cagcggttcc ttgtggagaa caggcaggct gtccacgccg gtgtattccc aggcggctac
     2041 ataggcgtat tcatcgtcgt tgcgcttggc ttcgccgtcc ggcagctggt attccacgcg
     2101 gaagtgcgcg ccgcaggatt cctgacggtt cagggcgtca tagcagagca gctgggcgaa
     2161 ttccaggaag tcggccacgc ggcaggcttt ttccagttcc gcgttgatgc cgtccgccgt
     2221 gccggttacg cgcacgtttt cccagaattc cttgcggagc tggggaatgc gttcaatggc
     2281 gtgggtcagg ctttcttccg tgcgggccat gccgcagtct tcccacatga tgaggcccag
     2341 ttcccggtgg aaggagtcca ccgtcttggt gcccttcaca ttcaggagct tgttgatgcg
     2401 ttccttcact tcgtcttccg cggccttgaa ttccggggag gcggtggtga cggcgcccgg
     2461 cttggtggtc gtcaggtagg cgggcagcgt ggctgggatg acgaagtacc cgtcagacag
     2521 gccctgcatc agggcggagg ctcccaggcg gttggcgccg tggtcggaga agttggcttc
     2581 accaaggacg tgcagcccct tcacgttgga catgaggttg tagtccaccc acaggccgcc
     2641 catggtgtag tgggaggcgg ggtagatcat catgggcgtt tcatgggggt cgtcgtccgt
     2701 gatttcttcg tacatgtcaa acaggttgcc gtactggccg tcaatccatt cgcggcccat
     2761 gcgcttgatt tcatccgcaa agtccaggaa gacgcccttg ccggtgacgg ccacgccgcg
     2821 gccgtcgtcg caggcttcct tggcggcgcg ggaggaaatg tcgcgcggag ccaggttgcc
     2881 gaaggaggga tacttgcgtt ccaggtagta gtcccgttct tcttcgggaa catcggaagg
     2941 cttgatttcc tttttgcgga ttttttccgc ggtttcacgg gacttgggca cccagatgcg
     3001 gccgctgttg cggagggatt ccgacatcag cgtcagtttg gactggtagt cgcctttgac
     3061 cgggatgcag gtcgggtgga tttgcgtgaa gcaggggttg gcgaagaagg caccacgctt
     3121 gtaggctttg taggcggcgg tgacgttgca gcccatcgcg ttggtggaca ggaagaacac
     3181 g
//
LOCUS       DAOMSU010000244         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3085_length_3173_cov_3.577614, whole genome shotgun sequence.
ACCESSION   DAOMSU010000244 DAOMSU010000000
VERSION     DAOMSU010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3085_length_3173_cov_3.577614"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..496
                     /locus_tag="PK077_08965"
     CDS             167..496
                     /locus_tag="PK077_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24468.1"
                     /translation="MDEEATATGRNHGEQPLDELMKRWHLTNHDLVEISPEQLTHKQV
                     QKARQGRQLTLKMMQKVCRALNVAIWERLTPMQKEQYFEYMHKHVFSYAKGYDPAWKD
                     PNMDMMA"
     gene            complement(509..1318)
                     /gene="mazG"
                     /locus_tag="PK077_08970"
     CDS             complement(509..1318)
                     /gene="mazG"
                     /locus_tag="PK077_08970"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRN24469.1"
                     /translation="MNDTEMIECREPALQMQRLIAIMKRLRAPHGCPWDAEQTHHSLI
                     SNMIEEAYEVVDTIQRNDWTQLREELGDVLLQVVFHAEIAQEAGRFDFNDVAAEVSEK
                     LVRRHPHVFAQSKADTTDAVLAQWDKIKRREKGAETTPYLHGTGKGLPPMLQAWKLQK
                     KAAKVGFDWADAQGALDKVKEETAECGEILSAPEEDPRVAEELGDLLFSVVNLCRKKG
                     IDPETAMAGANRKFERRFNEMERLLAKDGLSLEEASAEAMEARWQQAKSAR"
     gene            1404..1706
                     /locus_tag="PK077_08975"
     CDS             1404..1706
                     /locus_tag="PK077_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24470.1"
                     /translation="MEVHTWKERTEEGTLVYRACHHAGKWKLECAPKVGRAERDDVEW
                     EHVELTRAHWVTLRDVLWRKYQRKRCPWRFIETIDKLLEEMPEEEGADSPDGKGGR"
     gene            1738..2487
                     /locus_tag="PK077_08980"
     CDS             1738..2487
                     /locus_tag="PK077_08980"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HRN24471.1"
                     /translation="MLRSVVREWLSWVYPFVCELCGRGGLDGCHVCPDCRGSFVPVEP
                     PFCAVCGEPAEGSFIPSGLCRRCAVALPSFEEARAVYVNTGSLRDLLLAFKYGGAVHL
                     AGSFAQMMAEAVRGNPHWFGGKKRLIVPVPMHCGKQARRGYNQAQELAVLLGEELGWP
                     YAGVLKRLPDTLPQASLSREQRLRHARKIYAADEKRMKRSPVRGRDVLLVDDVFTTGA
                     TADSCARLLLRSGAASVCVLTLARTHHAWRG"
     gene            2527..2958
                     /locus_tag="PK077_08985"
     CDS             2527..2958
                     /locus_tag="PK077_08985"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRN24472.1"
                     /translation="MAGMNPELMFHTEDEVMFYDTDCGGVVHNLAYLRMIEACRTKLG
                     AKLGMDYKTMSDLQQFAVVVRHEIDYVRPAVLGDTILTTGWLQSVDRARFWCDFEMRR
                     ASNNDLLVRAHQQLALVQMPQGRPVRIPAEWRARWLECMES"
     gene            3044..3119
                     /locus_tag="PK077_08990"
     tRNA            3044..3119
                     /locus_tag="PK077_08990"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3077..3079,aa:Lys,seq:ttt)
BASE COUNT          637 a          844 c          933 g          759 t
ORIGIN      
        1 cgtttgcgcc attccagtaa ttcctctttc tcgctaatca tcttattgca acggggcggg
       61 attgaaccat gtatccggac gccgggcaac tggaaatgca aaaaaagtgc tgatggggca
      121 atcatgtatt gcctatttcc cggaaatagg gcacgaaggt aggcgtatgg acgaggaagc
      181 cacagcaacc ggacgcaacc acggagaaca gcctttggat gaactgatga agcgctggca
      241 tctgaccaat cacgacctgg tggaaatttc tccggagcag cttacccaca agcaggtcca
      301 aaaagcccgg caggggcgcc agcttaccct gaaaatgatg cagaaggtat gccgtgcctt
      361 gaatgtagcc atctgggaac ggctcacccc catgcagaaa gagcagtact tcgaatacat
      421 gcacaagcat gtgttcagct atgccaaggg atatgatccg gcctggaaag acccgaacat
      481 ggacatgatg gcctgacccc ggattccgtt accgggcgga ttttgcctgc tgccagcgtg
      541 cctccatggc ctccgcagaa gcttcttcca gggaaagacc gtctttggca agcagccgtt
      601 ccatttcgtt gaaacgccgc tcaaattttc tgtttgctcc ggccatggcc gtttccgggt
      661 cgattccttt tttcctgcac aggttgacta cggaaaacag aagatccccc aattcttccg
      721 cgacgcgggg gtcttcctcc ggtgcggaaa gaatttcccc gcattccgcg gtttcttcct
      781 tcaccttgtc aagggcgcct tgggcgtccg cccagtcaaa tcctactttg gcggcttttt
      841 tctggagctt ccatgcctgg agcatgggcg gcagcccctt gcctgttcca tgcaggtatg
      901 gggttgtttc cgcccccttt tcccggcgtt tgatcttgtc ccactgagct aatacggcat
      961 ccgtcgtgtc cgccttggac tgggcaaata cgtggggatg gcggcggacg agtttttcgc
     1021 ttacttccgc agccacgtca ttgaaatcga aacgccctgc ttcctgggca atctccgcat
     1081 gaaaaaccac ttgaagcaaa acatcgccca attcttccct cagctgcgtc cagtcattcc
     1141 gctgaatggt gtccacgact tcataggctt cctcgatcat attggaaatc agggaatggt
     1201 gggtttgctc cgcgtcccag gggcagccgt ggggcgcgcg cagacgtttc atgatggcga
     1261 tgaggcgctg catttgaagg gcaggctcgc ggcattcgat catttcagtg tcgttcatga
     1321 cggagccata ctaatcgcgc aagggcggat gactagaaaa aattcctctt tacccgaaac
     1381 gcttcttcct tacactcgcc tccatggaag ttcacacgtg gaaagaacgc acggaagagg
     1441 gaaccctggt gtacagggca tgccaccatg ccggcaaatg gaagctggag tgcgccccca
     1501 aggtgggccg tgcggaacgt gacgatgtgg aatgggaaca cgtggaatta acgcgggcgc
     1561 actgggtaac cctgcgtgat gtgctgtggc gtaaatacca gcgcaagcgg tgcccgtggc
     1621 gtttcattga aaccatagac aagcttctgg aagagatgcc ggaagaggag ggtgccgatt
     1681 cccccgatgg gaagggcgga cgataggagc ctctttcaac tttctgccgt gcggggaatg
     1741 ctgaggtcgg ttgtcaggga atggctttcc tgggtgtatc cttttgtctg cgagttatgc
     1801 ggccgcggcg gcctggatgg ctgccatgtt tgcccggact gccgcggaag tttcgtgcct
     1861 gtcgagcctc ccttctgtgc cgtctgcggg gaaccggcgg agggttcctt catcccgtcc
     1921 ggattatgcc gtcgctgcgc ggtagctctc ccctcttttg aggaagcccg tgccgtgtat
     1981 gtgaatacgg gatcattgcg ggatcttctg ctggctttca aatatggggg agccgttcat
     2041 ctggccggct ccttcgcgca gatgatggca gaggcagtgc gtggaaatcc tcactggttc
     2101 ggcgggaaaa aacgcctgat cgttcccgtg cccatgcact gcggcaaaca ggctcgaagg
     2161 gggtacaacc aggcgcagga actggcggtt ctgctgggag aggaactggg ctggccgtat
     2221 gccggcgtgc tgaaacgcct gccggatacg cttccccagg ccagcttgtc gcgggagcag
     2281 cggctcagac atgcccgtaa aatttatgcg gcggatgaaa aaaggatgaa acgcagcccc
     2341 gtcaggggca gggatgtgct gctggtggat gatgttttta cgaccggagc cacggcagac
     2401 tcctgcgccc gtctgctcct ccgctccggg gctgcttccg tctgcgtgct gacgctggcg
     2461 cggacgcatc atgcatggcg cggctgaacc ggaattttct ttcagcccgc ctgtttcctt
     2521 tttacaatgg caggcatgaa tccggagctg atgtttcata cggaagatga agtcatgttt
     2581 tatgatacgg actgcggcgg ggtggttcat aacctggcct atttgcgcat gattgaggcc
     2641 tgccgtacca aacttggcgc taaattggga atggattaca aaaccatgag cgatttgcag
     2701 cagtttgccg tagtggtgcg ccatgagatt gactatgtgc gtccggccgt tctgggagac
     2761 accattttga cgacgggatg gcttcaatcc gtggaccggg cccgcttctg gtgcgatttt
     2821 gaaatgaggc gcgcttccaa taatgatctt ctggtcaggg ctcatcagca gctagccctg
     2881 gtccagatgc cgcaggggcg tcctgtgcgt attcctgccg aatggcgtgc gagatggctg
     2941 gaatgcatgg aatcctgatc tttttttgtc catcttccat tttacgcttg ccatgaaagg
     3001 gggtgtggtg tttactgctt gcgcccttcc agcaagggta agtgcttggg tagctcagtt
     3061 ggtagagcag tagactttta atctattggt cccgggttcg agtcccggtc caagtaccat
     3121 tccttttttg aaaccccgta agttcagcga cttacggggt ttattttttg tct
//
LOCUS       DAOMSU010000245         3173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3086_length_3173_cov_2.261065, whole genome shotgun sequence.
ACCESSION   DAOMSU010000245 DAOMSU010000000
VERSION     DAOMSU010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3086_length_3173_cov_2.261065"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..1399)
                     /gene="gdhA"
                     /locus_tag="PK077_08995"
     CDS             complement(44..1399)
                     /gene="gdhA"
                     /locus_tag="PK077_08995"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HRN24473.1"
                     /translation="MAQTYSQRVLDLVKAKHSHEKEFIQAVQEVLSTIEPVLAANSKY
                     EDHAILERIVEPERTILFRVPWIDDQGKVQVNRGYRVEFNSAIGPYKGGLRFHPSVNL
                     GILKFLGFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMTELQRHIGAD
                     TDVPAGDIGVGGREIGYLFGQYKRIRNEFTGVLTGKSLNWGGSLIRPEATGYGCVYFA
                     QNMLATRNDGLQGKVCLVSGSGNVAQYTVEKLNELGAKAVTMSDSNGYVYDPDGISPE
                     KLAWVMELKNKRRGRIAEYVKQFPKAQYFEGQRPWSVPCDCAFPSATQNEINGEDART
                     LIKNGCTLVAEGANMPTDLEGIETYLAAKILYGPAKAANAGGVATSGLEMSQNSQRLS
                     WTREEVDHKLKTIMANIHANAVAHAQEYSSDKSFTNYVTGANIAGFVKVADSMIDQGV
                     V"
     gene            complement(2003..3013)
                     /locus_tag="PK077_09000"
     CDS             complement(2003..3013)
                     /locus_tag="PK077_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRN24474.1"
                     /translation="MSRTLRIEYPGAVYHVQSEGNRGDAIYLDDEDREIFLRTFQEAS
                     RRSGWTVYAYALMANHYHILFQTARANLVDGMKWLQTAYTQRFNARHRMRGHLFAGRY
                     HAMVVEADNAHYFSTIIDYIHLNPARSGLARRHTFLSGCKWTSLPAWLASPAKRPKWI
                     HPERGLVCFGCDDTEDGRQKYLNHLMGRFEAERMDERSLLPAGHVGPGTVQRGWCYGS
                     SAFRARLVGELPRLARRKPVTTGMRASEIGEYQAEIIVKNGLKAFGLSEEELLVTPYS
                     HPSKLIIALAVRQSTLVPYAWISNRLHMGIPKSMGTLLHRAKKMAETDLKTRAWIERL
                     SS"
BASE COUNT          589 a          829 c          978 g          777 t
ORIGIN      
        1 ttctggtgtg ttttacattg gcagcaagta tgtcgggccg gggtcagact accccctggt
       61 cgatcatgga atccgccact ttcacgaagc cggcgatgtt ggcgccggtg acgtagttgg
      121 tgaaggattt gtcggaggag tattcctggg cgtgggccac ggcgtttgcg tggatgttgg
      181 ccatgatggt tttcagcttg tggtccactt cttcacgcgt ccaggagagg cgctggctgt
      241 tctgggacat ttccaggccg gaggtggcca caccgccggc attggcggcc ttggccgggc
      301 cgtacaggat tttggcggcc aggtaggttt caatgccttc caggtccgta ggcatgttgg
      361 cgccttccgc caccagggtg catccgttct tgatgagggt acgggcgtct tccccgttga
      421 tttcattctg cgtggcggag gggaaggcgc agtcgcacgg aacgctccag gggcgctggc
      481 cttcaaagta ctgggccttc gggaactgct tcacgtattc cgcaatgcgt ccgcggcgct
      541 tgttcttgag ttccatgacc caggccagtt tttccgggga aatgccgtcc ggatcgtaaa
      601 catagccgtt ggagtcggac atcgtgaccg ctttcgcgcc cagttcgtta agtttttcca
      661 ccgtgtactg ggctacattg ccggaaccgg acaccaggca gaccttgccc tgcaggccgt
      721 cgttgcgggt ggcaagcatg ttctgggcaa agtatacgca accgtagccg gtggcttccg
      781 gacggatgag ggagccgccc cagttcaggc tcttgccggt gagaacgccg gtgaattcgt
      841 tgcgaattct cttgtattgt ccgaacaggt agccgatttc gcggccgccc acgccgatgt
      901 cccccgcggg aacgtccgta tccgcgccga tgtggcgctg aagctccgtc atgaagctct
      961 ggcagaagcg catcacttca ttatcgctct tgcccttggg gtcgaagtcg gaaccgccct
     1021 tgccgccgcc catgggaagg gtggtgaggg agtttttgaa aacctgttca aaacccagga
     1081 acttcaggat accgaggttg acgctggggt ggaagcggag gccgcccttg tacggtccga
     1141 tggcgctgtt gaattccaca cggtacccgc ggttcacctg aaccttgccc tggtcatcga
     1201 tccacggcac gcggaacagg atggttcttt ccggttccac gatccgttcc agaatcgcgt
     1261 ggtcttcata cttggaattc gcggccaaaa ccggttcaat tgtgctgagc acttcctgaa
     1321 cggcctggat gaattctttt tcgtggctat gcttggcctt aaccaagtcg agcacgcgct
     1381 gggaatatgt ctgtgccatc ttttctgttg ttgtatttgg gttgacgctt tacgcgcagt
     1441 ttttcgcacg cgctttccgt ttatcattcc gcgaagcggg ctggcaatca tttttttccc
     1501 tggcggcttt caggggtcag taagaaaata gaaaatatgt ttatatgttg tgatttgtaa
     1561 actgaccccg ttcttgctga ttgacgcctg ccagaactgc gtgtttcgga tgctggggac
     1621 atggagtttg tttgcggtta ttcgggttcc tgtttccata aggcggattt tgaatccggt
     1681 gtccatgcag aatggatgac aaaggaaaaa attatcagga acagacaagg cggcagagat
     1741 ggaatgttac aaacaacgct gggtgggagc catcttggtt tccgcattgg tggcagaaag
     1801 ccggaattgc cgccatggct ttttcagtag ttccggaacc ggctgcggca tccgtgggat
     1861 tgcttgggcc ggtctgcatg atgatgtgcc ggagaagggg ctgaccttga gtgcttcctg
     1921 ccgcgggatg acggtttcat gcccggcgca tccttgtggc gggaggaaaa tttttcaaat
     1981 tattccgtgg gaggaacgga ggtcaggagc tcaggcgctc gatccacgcc ctcgttttca
     2041 aatccgtttc cgccattttt tttgcccggt ggagcaaggt tcccatggat ttgggaatgc
     2101 ccatgtgcag ccggttgctg atccaggcat acggtaccag cgtgctttgc cggacggcca
     2161 gggcgatgat gagcttggac gggtgactgt aaggcgtgac aagcagctcc tcctccgata
     2221 ggccgaatgc tttcaacccg tttttgacga tgatttccgc ctggtattcc ccgatttccg
     2281 acgcgcgcat ccccgtcgtg acgggctttc tgcgcgccag acggggaagt tcccccacca
     2341 gcctggcgcg gaaggcgctg gagccgtagc accagccccg ctggacggtg ccggggccaa
     2401 cgtggccggc gggcagcagg gagcgttcat ccatgcgttc cgcttcaaaa cggcccatca
     2461 gatggttgag gtatttttgg cggccgtctt ccgtgtcgtc gcagccgaag cagacgaggc
     2521 cgcgttccgg atgaatccat ttgggccttt tggcgggaga ggccagccat gcgggcaggc
     2581 tcgtccattt gcagccggaa aggaacgtgt ggcgtcgcgc caggccggac cgggccggat
     2641 tcaggtggat gtagtcaatg atggttgaaa aatagtgggc gttatccgct tccaccacca
     2701 tggcgtggta tctgcccgca aacagatgac cccgcattcg gtggcgtgcg ttaaaacgct
     2761 gggtataggc agtctggagc catttcatgc catctaccag attggcgcgc gcggtttgga
     2821 aaagaatgtg gtagtgattg gccatcaggg cgtaggcgta cacggtccat ccgcttctgc
     2881 gggacgcctc ctgaaaagtc cgcagaaaga tttccctgtc ttcgtcatcc aggtaaatgg
     2941 catctccgcg gttgccctcg ctctgcacgt gatagactgc tccggggtat tctattctca
     3001 gggtgcggct catgccggaa gcgtaccgga aaaggtctga aatgcaagtt tgttgttatt
     3061 tttaacctga cccctattgt gaaaaaagaa gaggaaaaaa atacgcttcc cggctcttcc
     3121 gcgttttccg gaggaagtaa attttgccgg agaggctccg ggcaaaggtc ttt
//
LOCUS       DAOMSU010000246         3162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3101_length_3162_cov_2.424203, whole genome shotgun sequence.
ACCESSION   DAOMSU010000246 DAOMSU010000000
VERSION     DAOMSU010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3101_length_3162_cov_2.424203"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="PK077_09005"
     CDS             complement(<1..930)
                     /locus_tag="PK077_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN24475.1"
                     /translation="MSDPLLEIKNLVTGFETESGLLKAVDGVSFTVPKGTCVGIVGES
                     GCGKSVTAMSIVRLLPQPMGKILDGQILFKGRDLVQAKETDMHGIRGRHIGVIFQEPM
                     TALNPVHSIGRQIGESLMLHRGMNTREARDAAIQLLQRVRIPAPEQRVDEFPHQLSGG
                     MRQRVVIAIALACHPELIIADEPTTALDVTVQAQILSLLKDLQAEMGSSSILITHDLG
                     VIAQSCDSVVVMYAGRVVEKAPVRELFANPRHAYTRGLLASIPQLSSVRKTKLPTIPG
                     QVASIADFVPGCRFCQRQGVPAEELTERPPLVEI"
     gene            complement(934..>3162)
                     /locus_tag="PK077_09010"
     CDS             complement(934..>3162)
                     /locus_tag="PK077_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRN24476.1"
                     /translation="LRSTLSSVMLSLLLAAGSGCKDSSQSQGVGWQPNVPFGTLPPGF
                     DSFPERWNKQINDRLAREEAAKQKEIRELRDKFFKEEDSKVREKLQSKLIADEAALSV
                     IHRRQTEGDYIKFKTPADIPQDLKWEDGLDNPEIGDPNAKKGGVLRQWAPGSYPDTFR
                     PNGPNSNSGFRGPLYDEIIIGLVSIHPVTGKIIPGIAHKWAESPDRRTVYFELDPDAR
                     YTDGAKVKAIDLLVNMYIRTSEYSRDVFYNNFFYQNASNITIYDDSRFSITLPFAKPL
                     LPYYCTLFIPSPPHFYCEFGPNYVERYQWRIPPTTGAYVVKPDGIIRGRQVTLQRVPD
                     WWARDKKFTKYMYNVDQIVYNFIAEPSKAIELFRIGELDVLNITKPELWHERMEIPEV
                     HNGYINRSTFYTIYPRPPYGVFLNTSKAPFNDLNVRRGFQHALNIQNIIDITFRGDYQ
                     RLNSYNSGFGKFTNPYIKARPYSPEQARAYFARAGYTIPCPDGILRKPDGTRLTAAIT
                     FPNSSPSLASTLGKLKEDARKCGLEIQLDPLDSTVAFRKIMEKRAQASFMAWGFTPPH
                     PMNEQGFHSRYAYDERGGLITYTNNICAYADKEMDKLLDAETNAATEDELQKATWKVQ
                     QKIDDEALWVPCWTTEFIRLGYWRWVKWPNSATTQFCHPVVFDPMESYLYWVDNDIKK
                     ETMEAKREGKTFEEVDTVYDQYRYMDSIESLDNKDGGGKLPSVPVIPESGDPLEPSAT
                     EK"
BASE COUNT          625 a          788 c          977 g          772 t
ORIGIN      
        1 tatttccacg aggggagggc gttccgtgag ttcttccgcg ggcacgccct gcctttggca
       61 gaagcggcat ccgggaacga aatccgcaat agaggccacc tgaccgggaa tggtgggcag
      121 tttggttttc cgcacggaac ttagctgagg gatggaggcc agcagtcccc tggtgtaagc
      181 atggcggggg ttggcaaaaa gttcccggac aggggccttt tccaccacgc ggccagcgta
      241 catgacgact acggagtcac agctctgtgc gatgacgccc aggtcatgtg taatgaggat
      301 ggaggatgac cccatttccg cctgaaggtc tttgagcagg gacaggattt gagcctgaac
      361 ggtgacatcc agggccgtag taggttcatc cgcaatgatg agttccggat ggcaggccag
      421 ggcaatggct atgacgacgc gctggcgcat gccgccggag agctgatgag gaaattcgtc
      481 cacacgctgt tccggggcgg ggatacgaac gcgctgcagg agctggatgg ctgcatcccg
      541 ggcttccctg gtgttcattc cccggtggag catcagggat tccccaattt gcctgccgat
      601 gctgtgtacg ggattgagcg ccgtcattgg ttcctggaaa atgacgccga tatggcggcc
      661 gcggatgccg tgcatgtccg tctcttttgc ctgaaccagg tcgcggccct tgaacagaat
      721 ttgcccgtcc aaaattttcc ccatgggctg gggcagcagc cggacgatgg acatggccgt
      781 aacgcttttg ccgcagcctg attcccctac gatgcctacg caggtacctt tggggacggt
      841 gaagctgacg ccgtccaccg ctttcaacag gccggattcc gtttcgaagc cggtgaccag
      901 gttcttgatt tccaacaggg gatcgctcat gtgttatttt tccgtggcag aaggttccag
      961 agggtcgccg ctttccggga tgacaggcac agacggcagc ttgccgccgc catccttgtt
     1021 atccaggctt tcaatggagt ccatgtagcg gtactggtca tacacggtat ccacttcctc
     1081 gaatgttttt ccttcccgtt tggcttccat tgtttccttt ttgatatcgt tatccaccca
     1141 gtacagataa ctttccatgg gatcaaatac gacgggatgg cagaattgcg tagttgcact
     1201 gttcggccat ttgacccagc gccagtaacc cagtctgatg aattccgtgg tccagcaggg
     1261 aacccacagg gcttcgtcat caattttctg ctgcactttc cacgtggcct tttggagttc
     1321 gtcttccgtg gctgcgtttg tttctgcgtc caacagcttg tccatttctt tgtccgcgta
     1381 agcgcagatg ttattggtat aggtaataag gcctccacgc tcatcatagg cgtaccggga
     1441 gtggaacccc tgctcgttca tcggatgggg gggagtgaat ccccaggcca tgaaggaggc
     1501 ctgagcgcgt ttttccatga ttttgcggaa agctacggtg gagtccagag ggtcaagctg
     1561 gatttccagg ccgcacttgc gcgcgtcttc cttcagcttg cccagagtgg aagccaggga
     1621 aggagatgaa ttggggaagg tgatggctgc ggtgagccgg gtgccgtccg gcttgcggag
     1681 gataccgtcc gggcagggga tggtgtagcc cgccctggcg aaataagcgc gggcctgttc
     1741 cggggaatag gggcgcgcct tgatataggg attggtgaat ttgccgaaac ctgaattgta
     1801 ggaattgagc cgctggtagt ctccgcggaa ggtaatgtca atgatattct ggatattaag
     1861 tgcgtgctgg aacccccggc gaacattgag gtcattgaag ggggctttgg aggtattcag
     1921 aaaaactccg taaggagggc gggggtaaat ggtgtaaaat gtgctgcggt tgatgtagcc
     1981 gttgtgaacc tccggtattt ccatgcgttc gtgccatagt tccggtttgg tgatgttcaa
     2041 aacgtccagt tctccgatgc ggaagagttc gatggccttg gatggttccg caatgaaatt
     2101 atacacgatt tgatccacat tatacatgta tttcgtgaat tttttgtccc tggcccacca
     2161 gtccggcacg cgctggagcg ttacctggcg gccgcggatg ataccgtccg gcttgacaac
     2221 gtaagcgccc gtagtgggcg gtatgcgcca ctggtagcgt tccacgtagt tggggccgaa
     2281 ctcgcaataa aaatgcggcg gggacggaat gaacagcgtg caatagtacg ggagcagagg
     2341 tttggcgaag gggagtgtga tggaaaagcg gctgtcgtcg tagatggtga tgttggaagc
     2401 gttctggtaa aaaaagttgt tgtagaatac gtcccggctg tattccgaag tgcggatata
     2461 catgttcacc aggaggtcga tggcctttac cttggctccg tccgtatagc gggcatccgg
     2521 atccagttcg aagtaaacag tgcgcctgtc tggggattcc gcccatttat gagcgattcc
     2581 tggaatgatt tttcccgtaa ccggatggat ggaaacgagg ccgatgatga tttcatcata
     2641 cagggggccg cggaaaccgc tgttgctgtt cgggccgttg ggccggaatg tgtcgggata
     2701 ggatccgggg gcccactggc gcagcacgcc tccttttttg gcattcgggt cccctatctc
     2761 cggattgtcc agtccatcct cccacttcag gtcttggggg atgtccgccg gggttttgaa
     2821 tttgatgtag tcgccttccg tctgcctgcg gtgaatgacg gacagggcgg cttcatccgc
     2881 aatgagtttg ctttgcagtt tttccctgac tttagaatct tcctctttaa aaaacttgtc
     2941 ccggagctcc cgtatttcct tttgcttggc agcttcctca cgagccaggc ggtcattgat
     3001 ctgtttgttc catcgttccg ggaaggaatc aaatcccggt gggagtgtgc cgaagggtac
     3061 gttcggctgc cagcctaccc cctgggattg ggaggaatcc ttgcaaccgg acccggctgc
     3121 cagcagaagg gacagcatca cggaagaaag ggtggaacgg ag
//
LOCUS       DAOMSU010000247         3157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3110_length_3157_cov_2.082205, whole genome shotgun sequence.
ACCESSION   DAOMSU010000247 DAOMSU010000000
VERSION     DAOMSU010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3110_length_3157_cov_2.082205"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            279..1460
                     /gene="tyrS"
                     /locus_tag="PK077_09015"
     CDS             279..1460
                     /gene="tyrS"
                     /locus_tag="PK077_09015"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRN24477.1"
                     /translation="MTIDEQLDILMGGTAVVISREELKERLKLGRPLRVKLGVDPTAP
                     DIHLGHTVAIEKLRQFQELGHQAVLLIGDFTATIGDPSGRSVTRPPLSREQVLENAET
                     YTKQAFKILDRDKTEIVYNGDWFRKMTYEEVLKLNSRVTMQQMLAREDFKARVEGGKE
                     VRLHEMQYPIMQGWDSVEIRADVELGGTDQLFNILVGRDLQKEEGMLPQIAMTMPLLE
                     GLDGVRKMSKSYGNYVGVDEAPEMMFGKMMSASDELMDRYYLVLLGEKRDMGLHPMEA
                     KKLLAWKITARYHDSAAADAARSDWETRFSKRDLAAADLPEVEIASLPAGMNALALVS
                     FLFENVFQVKKSNGVLRKEHFTPGAIQLNDVKMTDPSTVLELAPGNVLRLSKKHAVRF
                     K"
     gene            1497..2477
                     /locus_tag="PK077_09020"
     CDS             1497..2477
                     /locus_tag="PK077_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HRN24478.1"
                     /translation="MPGFLAKLDRFTIGLIVSVGAGILVPCSGVWDTVFSRLSDAAIV
                     LLFFLYGAKLSRRSVWEGLMHWRLQGLVAASTFVIFPLLGMVSMPLWNSVLGPDLCMG
                     MLYVCMLPSTVQSSIAFTSMAGGNVAAAICSASVSSLLGVFLTPLLVGLVWSRGGEGG
                     VDFSTFLNICYIILVPFVAGQFAQRWIGKWVVTHRNLTSWTDHSTIWLVIYTAFSHAM
                     INGVWENLPWLSLVEVLAFCGLLLAAALWLTAFLSRRLRFSREDRIAIIFCGSKKSLA
                     TGIPMMNVIFAGAPIGLLVIPIMVFHQLQLMVCSFLARKWAKDVGKGEQL"
     assembly_gap    2584..2659
                     /estimated_length=76
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2660..2871)
                     /locus_tag="PK077_09025"
     CDS             complement(<2660..2871)
                     /locus_tag="PK077_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24479.1"
                     /translation="MHISPLRKNIPLTVVTLAALGIMFFLFFMHPASTEESLEFYAPD
                     GNIAGSFRVEITHAPTSPAYTEVTIIP"
BASE COUNT          580 a          819 c          957 g          725 t
ORIGIN      
        1 cctatccccg cctgtaccag ctcctgtaga ttcgcgggga catttctctg catccggaag
       61 ggagcagggc aggtccgtga catggaacag gaggaagggc catgcgggga aaatgccagc
      121 accggcgtgc cgtttattcc gcctttcggg acggattccg ttcctgttcc ctcacgtcgg
      181 aaccggtggc gggggattct tggccgcgtt cgatgagccg cgcacaggat ataggcttga
      241 aaaggcgcgg tgcccccggc actctatgcg ccagatcaat gactatagat gagcaattag
      301 acatattgat gggcggtacc gccgtcgtga tcagccgcga agagctgaag gagcgcctca
      361 agctgggccg ccccctgcgc gtgaagctgg gcgtggaccc tactgcgccg gacatccacc
      421 tgggccatac cgtggctatt gagaaattgc gtcagttcca ggaacttggc caccaggctg
      481 ttttgctcat cggagatttc accgccacga tcggcgaccc ttccggccgt tccgtgaccc
      541 gcccccccct ttcccgtgaa caggtgctgg agaatgcgga gacatatacc aagcaggcgt
      601 tcaagattct ggaccgtgac aagacggaga ttgtgtacaa tggggactgg ttccgcaaga
      661 tgacgtatga ggaggtgctg aagcttaatt cccgcgtgac catgcagcag atgctggccc
      721 gggaggattt caaggcccgt gtggagggcg gtaaggaggt gcgcctgcat gagatgcagt
      781 atccgattat gcagggctgg gattccgtgg aaatccgtgc ggacgtggaa ctgggcggga
      841 cggaccagct tttcaacatc ctggtgggcc gcgaccttca gaaggaggaa ggcatgctgc
      901 cgcagatcgc catgacgatg cctcttctgg aaggtctgga cggcgtgcgg aagatgtcca
      961 agtcctacgg gaattacgtg ggcgtggatg aggctccgga gatgatgttc ggcaagatga
     1021 tgagcgccag cgacgaactg atggaccgct attacctggt gctgctgggt gagaagcggg
     1081 acatgggatt gcatccgatg gaagccaaaa agctcctggc ctggaaaatc acggcccgct
     1141 atcatgattc cgccgctgcg gatgccgcgc gttctgactg ggaaacccgt ttttccaaga
     1201 gggatttggc cgccgcggat ttgccggaag tggagattgc ctccctgcct gccggcatga
     1261 atgccctggc tctggtttcc ttcctgtttg agaatgtttt ccaggtgaaa aaatccaatg
     1321 gcgttctccg caaggagcat ttcacgcccg gtgccatcca gttgaatgat gtgaaaatga
     1381 cagacccctc caccgttttg gaactggctc cgggcaacgt cctgcgcctg agcaagaagc
     1441 atgctgtgcg tttcaaatag aacgccggag gcggtattta ccttttttcc ttttccatgc
     1501 cgggattcct tgccaaattg gatcgcttca ccatcggcct catcgtcagt gtgggcgcgg
     1561 gaattctggt tccctgttcc ggagtatggg atacagtgtt cagccgcctg agcgatgcag
     1621 ccatcgttct gctttttttc ctgtacgggg ccaagctttc ccgccgttcc gtatgggaag
     1681 ggttgatgca ctggcgcctt caggggctgg tggctgcgag cacctttgtt attttccccc
     1741 tgctgggcat ggtcagcatg cccttgtgga atagtgtgct ggggccggat ttgtgcatgg
     1801 gaatgctgta tgtgtgcatg ctgccttcca ccgtgcagtc cagcatagcg ttcacttcca
     1861 tggcgggcgg caatgtggcc gctgccattt gctctgcttc cgtttccagc ctgctggggg
     1921 tgttcctcac ccccttgctg gtggggctgg tgtggtcccg cggaggggaa ggaggcgtgg
     1981 atttttccac tttcctgaac atctgctata ttattctggt gccttttgtg gccggccagt
     2041 tcgcccagcg ctggattggc aaatgggtag tcacgcaccg gaacctcact tcctggacag
     2101 accacagcac catctggctg gttatttaca cggcattcag ccatgcgatg attaacggcg
     2161 tatgggaaaa tctgccctgg ctttccctag tggaggtgct ggctttttgc ggcctcctgc
     2221 tggccgcggc cctgtggctg acggctttcc tttcacgcag gctccgtttt tcccgcgagg
     2281 accgtattgc gattattttc tgcggctcca aaaagagcct ggctaccggc attccgatga
     2341 tgaacgtgat ttttgccgga gcccccatcg ggctgctggt gattcccatc atggtgttcc
     2401 accagctcca gttgatggtt tgctccttcc tggcccggaa gtgggcgaag gacgtgggaa
     2461 agggagaaca actttaagag ggcctctgtt tgcccgccct ctcaatgtcc ggtcaggcgc
     2521 aggacgccgt cgtattgcat gtcccggccc ggaacaatga tgtggctgcc gcgggaggga
     2581 attnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnng ggatgattgt tacttccgta taggcggggc tggtgggcgc
     2701 atgcgtgatt tccacccgga aggagcctgc gatgttgccg tccggcgcat agaattccag
     2761 tgattcttcc gtagaggcgg gatgcatgaa gaacaggaaa aacatgatgc ccagggccgc
     2821 cagggtgacg acagtcaacg gaatattttt tctgagggga ctgatatgca tgccttcttt
     2881 tcctttcttc cataagatta tgggttttgc cggagcgtgt caacccctcc gggggacagg
     2941 cgatgatgca tttcttcccc atattctttt caaggaacct gttccggaag aacgggagac
     3001 ggaaaaaggg ttgcgtccgc gggacaatgt ggtagcttgt gcgggcaaag ggacccctat
     3061 gaccttacct gtaaagattc tgtccgtatt ggcagggctg agcctggcgg gaagcgcccg
     3121 ggcgcgcatt tggacgaatg accggggcgc taccgtc
//
LOCUS       DAOMSU010000248         3148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3122_length_3148_cov_2.526027, whole genome shotgun sequence.
ACCESSION   DAOMSU010000248 DAOMSU010000000
VERSION     DAOMSU010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3122_length_3148_cov_2.526027"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..170
                     /locus_tag="PK077_09030"
                     /pseudo
     CDS             48..170
                     /locus_tag="PK077_09030"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
     gene            187..903
                     /locus_tag="PK077_09035"
     CDS             187..903
                     /locus_tag="PK077_09035"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:NF023246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HRN24480.1"
                     /translation="MNPPSGRGLCIGQYLPFFAARDRAGLLKVTTQKFYRVAGGSTQL
                     KGVESDIQLPTATAAFELGEDILDYAMPYDQITPCTNYKKDSSIAAMLPVLKDASAKR
                     VEKDRDLQIAREDIAMMKQRIKDNKLSLNKKIREQENSALEERRKSINKERKIRFAEM
                     AKEDATKYKIYRLTLDDVNAKELPLADPEKDNEQFMHLAEDPTAELDDSPEYPSGLDP
                     ELREGINIVQDMLKLESSGK"
     gene            1078..2766
                     /gene="rpiB"
                     /locus_tag="PK077_09040"
     CDS             1078..2766
                     /gene="rpiB"
                     /locus_tag="PK077_09040"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRN24481.1"
                     /translation="MNTTYKIAIGADHAGVDLKETVAELLKAWGHEVTDMGTMTKNSC
                     DYSDYANAVASSIADGTNDRGILICRSGIGMSIAANRWVGVRAALCRTAPAAALSRQH
                     NDSNLLCLASAYVDNESVEDVLDAWLHADFEGGRHERRVVKSSGSPLQWTDPELAGLI
                     QEEGRRESNNIELIASENFTSPSVREAQGSLLTNKYAEGYPGKRWYGGCEVVDKVEQL
                     AIDRVLKIFGGDHANVQPHSGSQANMAVYFSVLKPGDTILTMDLSHGGHLTHGHRANF
                     SGKLYNVVHYGVSQETEAIDYDALEKLALEVKPQMITAGASAYSRTIDFERMGQIARA
                     CGAYLFVDMAHIAGLVAGGQHPNPVPHADFVSSTTHKSLRGPRGGFVICKEEYAKKLD
                     AAVFPGMQGGPLMHIIAAKAACFGEALKPEFKDYAAQVVKNAKAMAAKMAELGFRVVS
                     NGTDNHVFMVDLRNKGINGADAQEALDRVGITVNKNAIPFDTGSPMKPSGIRIGTPAV
                     TTRGMKEKDVEQVAEFIARALALYVGDQVCPNPEGFSQLKEEVSAFNRNFRLPH"
     gene            complement(2966..>3148)
                     /locus_tag="PK077_09045"
     CDS             complement(2966..>3148)
                     /locus_tag="PK077_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24482.1"
                     /translation="DEKNIQFSNEGFKIILIADGGATRASHQFHYSQPAAVFVFRLGR
                     EEGKCSSEDVRMNNDC"
BASE COUNT          774 a          973 c          811 g          590 t
ORIGIN      
        1 ttaccggaaa cggccccgtg gtgcaaatca aggacacccg cggcaacgtg gatatcaaat
       61 ccgcccacaa ccgccagaaa ctcttcaatg gccccattgt ggtgctcatt aataaactca
      121 gcgcatccgc ctctgaaatt ctggccgcgg cccttcagga ttacggccgc gccgtgattg
      181 tgggggatga atccaccttc gggaaggggt ctgtgcatcg gccaatacct gcctttcttc
      241 gcggccagag accgtgcggg cctgctgaaa gtcactaccc aaaaatttta ccgtgtggcg
      301 ggcggctcca cccagctcaa aggcgtggaa agcgatatcc agcttcccac cgctacggcg
      361 gcattcgagc tgggagaaga cattctggac tacgcgatgc cctatgacca gattacgccc
      421 tgcaccaact acaaaaagga ctcctccatc gcggccatgc tgcccgtgct gaaagatgcc
      481 agcgcgaagc gcgtggaaaa agaccgcgac ctccagattg ccagggaaga tatcgccatg
      541 atgaaacagc gcatcaagga caacaagctt tccctgaaca agaaaatccg ggaacaggaa
      601 aactccgccc tggaagaacg ccgcaaatcc atcaacaagg aacgtaaaat ccgcttcgcg
      661 gaaatggcca aggaagacgc aaccaaatac aaaatttacc gcctgacgct ggatgacgtc
      721 aacgccaagg agctgcccct ggcggatccg gaaaaagaca atgaacaatt catgcacctg
      781 gcggaagacc ccacggcaga actggacgac tccccggaat acccctccgg ccttgatccg
      841 gaactccgcg aaggcatcaa catcgtccag gatatgctga agctggaatc ctccggaaaa
      901 taacggctcg ctcctcgcga acctccttgc cgccccggcc ccattcaggg aaccggggtt
      961 cttttttccg aggcaattcc gggagccgtg cctcccgaac agcccggaat gaatccgggg
     1021 cttgccagcc ggagggaaat aaggcagtat ttttgcccgt cagcaatgag caccaccatg
     1081 aatacgacat acaaaattgc catcggcgcg gatcacgccg gagtcgatct caaggaaacc
     1141 gtagcggaac tcctcaaggc atggggtcat gaagtgaccg acatgggaac catgaccaaa
     1201 aattcctgtg attattccga ctacgccaat gcagtcgcaa gcagcattgc tgacggcacc
     1261 aacgaccgcg gcatccttat ctgccggtcc ggcatcggca tgagcattgc cgccaaccgc
     1321 tgggtgggcg tccgcgccgc cctctgccgc acggcccctg ctgcggccct ttcccgccag
     1381 cacaatgatt ccaacctgct ctgcctggct tccgcctatg tggacaatga aagtgtggaa
     1441 gacgtcctgg acgcctggct gcatgctgac tttgaaggcg gacgccatga acgccgcgtc
     1501 gttaaatcct ccggtagccc cctgcaatgg acggacccgg aactggcggg cctcatccag
     1561 gaggaaggac gccgggaaag caacaacatt gagctgattg cctccgaaaa tttcacctct
     1621 ccctccgtcc gggaagccca gggctccctg ctgaccaaca aatacgcgga aggctatccc
     1681 ggcaagcgct ggtatggcgg ctgcgaggtg gtggacaaag tggaacagct cgccattgac
     1741 cgcgtgctga aaattttcgg cggggatcat gccaacgtgc agccccactc cggttcccag
     1801 gccaacatgg ccgtttactt ctctgttctg aagccgggcg acaccattct cacgatggat
     1861 ctctcccacg gcggccacct cacccacgga caccgggcca acttctccgg caaactgtac
     1921 aatgtagttc actacggcgt atcccaggaa acggaagcta ttgactatga tgctttggaa
     1981 aaactggctc tggaggtaaa gccgcaaatg atcacggcgg gagcaagcgc ctactcacgc
     2041 acaatcgact ttgaacgcat gggccagatt gccagggcct gcggtgccta cctgttcgtg
     2101 gacatggccc acatcgcggg cctggtggcc ggggggcagc acccgaatcc ggttccccat
     2161 gcggacttcg tgtcctccac cacgcataaa tccctgcgcg gcccccgcgg cggcttcgtc
     2221 atctgcaagg aggaatacgc caaaaaactg gatgcagccg tcttccccgg aatgcagggc
     2281 ggccccctca tgcacatcat tgccgccaag gcggcctgtt tcggagaagc tctgaagccc
     2341 gaattcaagg actatgcggc ccaggttgtc aaaaacgcca aggccatggc ggccaaaatg
     2401 gcggaactcg gcttccgcgt cgtctccaac ggtacggaca accacgtatt catggtagac
     2461 ctccgcaaca aaggcattaa cggtgcagac gcgcaggaag ccctggaccg cgtaggcatc
     2521 accgtaaaca agaacgccat cccgtttgac acgggttctc ccatgaaacc ctccggcatc
     2581 cgcatcggca cgcctgccgt aaccactcgc ggcatgaagg aaaaagacgt ggaacaggtg
     2641 gctgaattca ttgcccgtgc gctggccttg tacgtaggtg accaggtatg cccgaatccg
     2701 gaaggcttct cccagcttaa ggaagaagtt tccgcgttta accgcaactt ccgcttgcct
     2761 cattaacatc tcctgcgtat aaacgctttt caggccgttc cgccgtttcc gcggaacggc
     2821 cttttaatta aaaatccgta ttcacggtcc ctcaatacgg acggccaaga cacccgcaac
     2881 aaaaaaactc ttcgtcaagg aatagccagg aattgtccaa actgcgcttt tcctctccta
     2941 tattacggac aaaccggttt ggaaatcaac aatcattatt catcctcacg tcctcgctgg
     3001 aacatttgcc ctcttcacgg cccaaccgga aaacaaagac agcggcaggt tgggaataat
     3061 ggaactgatg gcttgcacgg gttgcccctc catccgcaat taaaataatc ttaaaacctt
     3121 cattggaaaa ctggatattt ttttcatc
//
LOCUS       DAOMSU010000249         3145 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3129_length_3145_cov_2.734951, whole genome shotgun sequence.
ACCESSION   DAOMSU010000249 DAOMSU010000000
VERSION     DAOMSU010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3129_length_3145_cov_2.734951"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(678..1265)
                     /locus_tag="PK077_09050"
     CDS             complement(678..1265)
                     /locus_tag="PK077_09050"
                     /inference="COORDINATES: protein motif:HMM:NF021363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HRN24483.1"
                     /translation="MEHRYRQLMRKVRLYLLTEVRKKSWASKFLSASVFDSVYWSWNR
                     QSVATGAGWGAAAAIAPLPMQSLWGVFACLWRKGNIPVAILMAWLSPPGFTFFAIPGQ
                     WWLGWFLFSSVGIPTSGANWQMLKTGVQQWSWAPFDGLSIGMVSLEFLTGWIVSSVVL
                     GFLCYGLVQLAWGAGHFLRSRKNPGKPSSGKGFGK"
     gene            1426..2154
                     /locus_tag="PK077_09055"
     CDS             1426..2154
                     /locus_tag="PK077_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRN24484.1"
                     /translation="MSKQRLDILLVSRNLVESRELAQRLIIAGEVSVGGHPSTKPGLK
                     VNEDADIAIRNRPRYVSRGGLKMEGALNAFPVSAEGKVCLDIGASTGGFTDCLLQHGA
                     ARVHAIDVGTNQLVWKLRQDPRVIVKEKFNARYMTPEDIGEQVDLIVSDVSFISLKKI
                     LPAAFPLLKKEGDALVLIKPQFELQPEDIGPGGIVRDPALHQRAVDSIRTFVTEELNR
                     SWMGCEPSPITGTDGNHEFLAWLK"
     gene            2227..2586
                     /locus_tag="PK077_09060"
     CDS             2227..2586
                     /locus_tag="PK077_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24485.1"
                     /translation="MADNGVECSVVMEKTRFDARETFPDFKLVFTNKGEKTVRLFDDF
                     YPLKDDGPNIHIEIWEKVGGKRYGYRVGKIENPIAYYVAGYKIERVAALIRFITLKPG
                     EKHESPHQGSLPVADAF"
BASE COUNT          802 a          843 c          757 g          743 t
ORIGIN      
        1 ctgatcaggc gtcaaatctt ttctgaccga caatgtaatt tttgccgttc ctttaagttt
       61 atcttctcca atttcttcca ccagctcctt ccctgaaaca aacgcccacg tcagtttttt
      121 aatatctccc ataggttttc ccgtcaacgt aagagtgaag gattctccta tgcctatgga
      181 acttctcttt ttatcgtctt cattttcttc tcccgtgata taacaatcag cggaataaag
      241 ggcagttttc gattcaatgg agagcgctcc cgtgtcccca tcaggcaacg gttcttcagc
      301 ctgggaacaa aaacagccca atagcgtgcc ccccccaaac caaagaaaga tcaggcagtg
      361 taaatatttc ttgaacaggg catcaataga gggagacatc atactcagga aagtttccca
      421 aaaataatat tttcaccata aacgtcaata ataaaacgga attatccact ttagtcttgt
      481 ttcgttgatc ttcccgattt ttcatcagag caattttctg aatatcaata gccagaatat
      541 tctgaaaatc ttacagcaag actattctat ataattccgt aataaaatga tactttcaaa
      601 ttccagaagt ccataaaata tatatctatc ccatgcagac gcttgcttcc tcctgcaaca
      661 catgacaggg ggcggcttta tttaccaaaa ccttttccgg aagaaggctt gcccggattt
      721 ttcctactgc gcaggaagtg gcccgctccc catgcaagct gcaccagccc gtagcacagg
      781 aaacccagca ctacgctgga aacaatccag cccgtcagga actccaggct taccatgccg
      841 atgctcaacc cgtcaaacgg agcccaggac cactgctgca cccccgtttt cagcatctgc
      901 cagtttgccc cggaggtggg aatccccacg ctggaaaaaa ggaaccaccc cagccaccac
      961 tggcccggaa tggcaaaaaa ggtgaagccg ggaggggaaa gccacgccat cagaatggct
     1021 acggggatat tccccttgcg ccacaggcag gcaaacacgc cccacaggct ctgcatgggc
     1081 agcggggcaa tcgccgccgc ggccccccag ccggcgcccg tcgctacgga ctgcctgttc
     1141 cacgaccagt aaacagaatc aaaaacactg gcggaaagga acttgcttgc ccacgatttt
     1201 ttgcgcacct ccgtcagcag gtaaagcctg actttgcgca tcaattgcct gtagcggtgt
     1261 tccatccgcc gcagagtctc ctataaaccg gccttgaatc aatcgtaaaa tgttccagta
     1321 cgaggaatgc cgcattttta cgcccgaatg cctctctgcg ctcggttttt cttgattctt
     1381 catgcggttt catgaaatac ctgcgttcac acctttttcc gaatcatgag caagcaacga
     1441 ctcgatatcc ttctggtttc ccgcaatctc gtagaatccc gggagctggc ccagcggctc
     1501 atcattgccg gagaagtaag cgtggggggg catccctcca ccaagcccgg tctcaaggtt
     1561 aatgaagacg cggatatcgc catcagaaac cgtccccgct atgtcagccg cggcggcctg
     1621 aaaatggaag gagccctgaa cgccttcccc gtttctgcgg aaggcaaggt ctgccttgac
     1681 attggggctt ccacaggagg cttcacggac tgcctgctcc agcacggagc cgccagagtg
     1741 catgccatag acgtaggaac caaccagctc gtctggaaac tgcgtcagga cccccgcgtc
     1801 attgtgaagg aaaaattcaa cgcccgctac atgactccgg aggatatagg ggaacaagtg
     1861 gatctgatcg tttccgacgt ctccttcatc tctctgaaaa aaatcctgcc agccgccttt
     1921 cccctgctga aaaaagaagg agacgccctg gtgctcatca agccccagtt tgaacttcag
     1981 ccggaagaca taggccccgg cggcatcgtc cgggatccgg ctttgcacca gcgggcggtg
     2041 gactccatac gcactttcgt cacggaggag ctgaaccgtt cctggatggg gtgcgaaccc
     2101 tcccccatca cgggaacgga cggcaaccat gaatttctgg catggctcaa atagctgcag
     2161 ccttttactg tccagttttt agtacttctg gcaagcctgt ccctccatgc tgcagcatgg
     2221 ggggacatgg ccgacaacgg agtggaatgt tccgtggtga tggaaaagac ccggtttgac
     2281 gcaagagaga cgttccctga cttcaaactg gtgttcacga acaagggcga aaagaccgtg
     2341 cgcctgtttg acgacttcta tcccctcaaa gatgacggcc ctaatatcca tatcgaaata
     2401 tgggaaaaag tgggagggaa aagatatggg tacagagtgg gcaaaataga gaatccaata
     2461 gcctattatg tagccgggta taaaatcgaa cgggtagcgg ctttgatacg cttcattacg
     2521 ttgaagccgg gagaaaagca tgaaagtccc catcaaggaa gcttacctgt tgctgacgca
     2581 ttttgaccgt cttcgcaatg gtcagaacta tgagctggaa gtatgtttca gggatggata
     2641 tgcggaaccc ggtgttcgga gggtgtatat agggaagact gaatttcata cgtcgagccg
     2701 gtcgcccaaa ggagtggctc ggtgaatgtt caacgtggat tcaatgagaa tggtgaaagt
     2761 ctcttttcat cacgtctgag gtgtgttgtt gcttctttga ggttcccggt aggatttgtc
     2821 atcaggttac acggccagtc acctgtgcat cagaggaata tttccctacc agcgaaaaat
     2881 accggtattc tcaagttttg atcaaggggc aggcagggta gactttgatg tggaatcatg
     2941 ggatggcaac aggtctgtga gaaactgttg acctcttcca cggtgcctga aggatttttc
     3001 cggaggattt tcttcatcac agcatgagtt tggccatcgc ccggagcatc cccaggaaac
     3061 aatccttacc ctgctgatta tagtagcgcg tttcgagttc acaagaacat tgtttccagc
     3121 cttccagatc gacttcatcc aggca
//
LOCUS       DAOMSU010000250         3142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3135_length_3142_cov_2.390023, whole genome shotgun sequence.
ACCESSION   DAOMSU010000250 DAOMSU010000000
VERSION     DAOMSU010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3135_length_3142_cov_2.390023"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..1105
                     /locus_tag="PK077_09065"
     CDS             92..1105
                     /locus_tag="PK077_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24486.1"
                     /translation="MSDLFEKKLFLSPAARRRGGKAGFALGRILSVIFFVIALGMFFG
                     GLFFWLQKRQASLDSGAAARICATPEMLSGELKAALKDRVDYSFWPPYHISPMEKQDL
                     LRNCLAEAQKYLSAADVVQKSSFVWKSLQSLPLMVRHYAMSPPEAIVSRPKGPKSFAV
                     FEVEGHPCAQLLVGFEKTPDMEFCFFKDEQDGSWKLDWQQFARFQPMNWEDFVRGKGE
                     DIAEFRVWMVRERMSENKDDYAFKLIAPGMNGSEERSIARPMVYVPRKSEMGKRLFML
                     FKINEEMEHSPYRVLNANDEADMLRARVLLSRSKEPNRKGEYSFTLVRLLGEGWYGLS
                     FPE"
     gene            complement(1156..1665)
                     /locus_tag="PK077_09070"
     CDS             complement(1156..1665)
                     /locus_tag="PK077_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24487.1"
                     /translation="MENYTKRDGDSALSDNCRQPPFKKPMNNNPAGNHLLACCAGSAI
                     SALLAFSSPVAEAGIHLHGEIRVPAAGQAVFLYDPSGFPIWGYSPCGRPIYAYTPAGA
                     PIYVIRDIYRGCHVPAWNPRPHYHGPFWPAGVCRRHHAARLTPCPPPFRPHRPRRPHE
                     GPPPRFHRR"
     gene            1738..2523
                     /gene="hisA"
                     /locus_tag="PK077_09075"
     CDS             1738..2523
                     /gene="hisA"
                     /locus_tag="PK077_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformimino-5-aminoimidazole
                     carboxamide ribotide isomerase"
                     /protein_id="HRN24488.1"
                     /translation="MTRFRPCIDLHGGRVKQIVGGTLTQDGVSLRTNFVSDRGAAWYA
                     DLYRRDGLSGGHVIKLGPGNDHVAREALAAWPGGLQVGGGMTPENAEEWISSGASHVI
                     VTSCLFDAEGTLRMDRLKDLVSAVGAERLVLDLSCRRVQGGWAVAMNRWQTLTELRVT
                     AETLDVLAEYCAEFLIHAADVEGLCTGIDRELVEMLGNWRRLPMTYAGGISRIQDIDE
                     IDALSGGTMDATVGSALDLFGGGLIRYGDLVARQHGKSGMETV"
     gene            2520..2810
                     /locus_tag="PK077_09080"
     CDS             2520..2810
                     /locus_tag="PK077_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRN24489.1"
                     /translation="MKLALKVIPNAKKSEAVGWEEDPRAGRALKLRIAAPPVEGKANK
                     VVVLFLSAWLDIPRSSISLLRGESSRLKVVELPDGCEGKLARLLSAEDISGA"
     gene            2945..>3142
                     /locus_tag="PK077_09085"
     CDS             2945..>3142
                     /locus_tag="PK077_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24490.1"
                     /translation="MSDEEKEKNAEAEELEVQEQVQDAPAEEKVAEPSLEEELLKWRD
                     TAMRTAAEYDNYRKRMVKEKEE"
BASE COUNT          645 a          737 c         1074 g          686 t
ORIGIN      
        1 gtctgttttt tatcgttgcg ctgccttggg cgttccggta gtgtgtgacc gtctttccgc
       61 gattgttttc tgtggatatt tccgttttga aatgagtgat ttatttgaaa aaaagctgtt
      121 cctttctccg gccgcgcgca ggcgcggcgg aaaagcgggc tttgcgctgg gccgcattct
      181 ttccgtcatc tttttcgtga tcgcgctggg aatgtttttc ggcggtcttt tcttctggct
      241 ccagaagcgc caggcatccc tggattccgg agcggccgcg cggatttgcg ccacgccgga
      301 gatgttgtcc ggagaattga aagccgctct gaaagacagg gtggactata gtttctggcc
      361 tccttatcac atttctccga tggaaaagca ggacctgctg cgcaactgcc tggcggaggc
      421 acagaaatat ctcagcgccg cggatgtggt gcaaaaatcc tcttttgtgt ggaagtccct
      481 gcaatcgctt cctttaatgg tcaggcatta cgccatgtct ccgcctgagg cgattgtcag
      541 caggcctaag gggcctaaaa gctttgccgt gtttgaggtg gaggggcatc cctgcgccca
      601 gctcctggtt ggttttgaaa agactcccga tatggaattc tgttttttca aggatgaaca
      661 ggatggttcc tggaagctgg actggcagca gtttgcccgc tttcagccga tgaactggga
      721 ggattttgta cggggaaaag gggaggatat tgcggaattc cgcgtatgga tggttcggga
      781 gcgcatgtcg gaaaataagg atgactacgc atttaagctg attgctcccg ggatgaacgg
      841 ttccgaagag cgcagcattg cccggcccat ggtgtatgtc cccagaaaat cggaaatggg
      901 caagcgtttg ttcatgttgt ttaagatcaa tgaggagatg gaacattccc cataccgggt
      961 gttgaacgcc aatgacgagg ctgacatgct ccgcgcgcgg gttctccttt cccgcagcaa
     1021 agagcccaac cgcaaggggg aatattcctt tacgctggtc aggctgctgg gggaaggctg
     1081 gtacggcctt tctttcccgg aataaaaagg aaggattggg ccggcctctt gctttgcgga
     1141 ggatggggat gcgttttacc ggcggtggaa acggggaggg ggcccttcat gtgggcgcct
     1201 ggggcggtgg ggccggaaag ggggcgggca tggcgtaagg cgtgcggcat gatgtctgcg
     1261 gcagacgccc gccggccaga aagggccgtg gtaatgcggc ctggggttcc atgcaggaac
     1321 atggcagccg cggtagatgt ccctgatgac gtaaatgggt gcgcctgcag gggtataggc
     1381 gtaaatgggc cgtccgcatg gggaatatcc ccagatgggg aatccggagg ggtcatacag
     1441 gaaaacggcc tgccctgccg caggtacgcg gatttcgcca tgcaggtgaa ttccggcttc
     1501 cgcgacgggg gaagaaaagg ccaggagggc agatatggcg cttccggcgc agcaggcaag
     1561 aagatggttt ccggcagggt tgttgttcat cggttttttg aatggtggtt gacggcagtt
     1621 atccgataga gcggagtccc cgtcccgttt tgtgtaattt tccatgcccg tcattcaggg
     1681 aaggggtaaa gggaataccg tcttgacctg gaggggcgcc ccgggcaaag tgaccgcgtg
     1741 acgagattca gaccatgcat tgatttacac ggcgggcgcg tgaagcagat tgtgggcgga
     1801 accctgacgc aggatggcgt ttcattgcgc accaatttcg tctccgaccg cggcgcggca
     1861 tggtatgcgg atttgtaccg cagggacggt ctgagcggag ggcatgtgat caagctgggc
     1921 ccagggaacg accatgtggc gcgggaggcc ctggcggcat ggccgggagg ccttcaggtg
     1981 ggcggcggca tgacgccgga aaacgcggag gaatggattt cctccggggc cagccatgtg
     2041 attgttactt cctgcctgtt tgatgcggag ggaacgctgc ggatggacag gctgaaggat
     2101 cttgtttccg cagtgggcgc ggaacggctg gtgctggatt tgagctgccg cagggttcag
     2161 ggaggctggg ccgtggccat gaaccggtgg cagactttga cggaactccg ggtaacggcg
     2221 gagacgctgg atgttctggc ggaatattgc gcggagtttc ttattcatgc ggcggatgtg
     2281 gaagggctct gcaccggcat tgaccgggaa ctggtggaaa tgctgggaaa ctggcgccgc
     2341 ctgcccatga catatgccgg aggcatcagc cgcattcagg atattgacga aatagacgct
     2401 ttgagcggcg gaaccatgga tgccacggtg gggagcgctt tggatttgtt tggcggcggt
     2461 ttaatcaggt atggggatct ggtggccagg cagcacggga agtccggcat ggaaacggta
     2521 tgaaactggc tctgaaagtt attcccaatg cgaagaagag cgaggccgtg ggatgggagg
     2581 aagatccccg tgccggccgc gcgctgaagc tgcgcattgc cgcgcctccc gtggagggaa
     2641 aggcgaacaa ggtcgtcgtc cttttcctgt ccgcatggct ggacattccc cgttcctcca
     2701 tcagcctgct gcgcggggaa tcctcccggc tgaaagtggt ggaactgccg gacggatgcg
     2761 agggaaaact tgcccggctg ctttctgcgg aggatatttc cggagcttag gaaagccggg
     2821 cggaaggcag gcattttctt tatgcgtaga agggttgcat caggcgaggt ccattacgcg
     2881 tcaaacgtca tccatgtctt agcaggccgt tgacgcatcc ggtaaaagaa tttacattgc
     2941 ccgcatgagc gacgaagaga aagagaaaaa cgcggaagcg gaagagctgg aggtgcagga
     3001 acaggtgcag gatgctcctg ccgaggagaa ggtggcggaa ccttccctgg aagaggaatt
     3061 gcttaaatgg cgggataccg ccatgcgcac tgctgcggaa tatgataact accgcaagcg
     3121 catggtgaag gaaaaggagg aa
//
LOCUS       DAOMSU010000251         3131 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3151_length_3131_cov_2.500650, whole genome shotgun sequence.
ACCESSION   DAOMSU010000251 DAOMSU010000000
VERSION     DAOMSU010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3151_length_3131_cov_2.500650"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1053)
                     /locus_tag="PK077_09090"
     CDS             complement(<1..1053)
                     /locus_tag="PK077_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24491.1"
                     /translation="MSSNAPSPAPSRKPKPVPERFQVAKTTLWFDRIMTKTIIGGGFT
                     VIVAVFGILFFLLAVTIPLFQSADVEERQHPAPSSPVPGTWGLDPSGTLPFVYGNGKN
                     IFFLDKTTGNLSSVPVTLPEGETVCAHSYNASLTAYPIATESGKVGLIHVHSGLNVHG
                     RTNAHGPDKAGAEAGPLYPLTENGSVPGKISGIAYADAGERKIFTATVETEQGTRLLL
                     MTLEESRSLLHEGELAPSGFHDLTDQLEGRPTAMLPGASGDSLVIATDADKLLYFSYD
                     EDGETWVRRQMIPTPLGKGEKMTSVNWLFGDMSLVIGGDKGSLKIFSLYPHTRPDGTS
                     LRLFGQTRQFSPMDGPV"
     gene            complement(1145..2107)
                     /locus_tag="PK077_09095"
     CDS             complement(1145..2107)
                     /locus_tag="PK077_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HRN24492.1"
                     /translation="MKFVAKILTIAAALSSLSMGSSQVTVDNSIKPYAPSSGVSGNLN
                     AVGSDTLNNLMTLWAEGFSKKYPSVKIGVEGKGSSTAPPALTAGTAQLAPMSRQMKRE
                     EIAAFEAKYGYKPTEIKVALDAVAFFVNKNNPIQALTLAQIDSIFSSTFKRGGSNITD
                     WGDAGVPSMKGKAISIYGRNSASGTNGFVKEIALKKGDYKNSVKEQPGSSAVVQGISS
                     DEQGIGYSGIGYVTSGVKTLSLAEKSGKAAVQPSYDNCINGTYPLSRYLLIYVNKKPG
                     EPLDTLTREFIKFIVSKDGQEIVTKDGYYPIPAKVSAEVLKSIE"
     gene            2312..3094
                     /gene="pstB"
                     /locus_tag="PK077_09100"
     CDS             2312..3094
                     /gene="pstB"
                     /locus_tag="PK077_09100"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRN24493.1"
                     /translation="MTPHPAAADQTAVEVDSLNFCYGSKQALFNVTMKIPKNRVTAFI
                     GPSGCGKSTLLRCINRMNDRIPEARVTGGSIRIDGVDVTSPSLDAIRLRREVGMVFQK
                     SNPFPMSIYENVAYGLKLAGVKKRGILDEAVEESLRHAALWDEVKDRLHASANGLSGG
                     QQQRLCIARSIAVKPRILLMDEPCSALDPIATVRVEELMHRLKDKFTIIVVTHNMQQA
                     TRVSDLTGFFMLGRLVEFDATEKIFSNPSLKETEDYITGRFS"
BASE COUNT          611 a          784 c         1001 g          735 t
ORIGIN      
        1 caccggaccg tccatgggag agaattgcct ggtctggccg aagaggcgca gggaggtgcc
       61 gtccggacgg gtgtggggat aaaggctgaa aattttaaga cttcccttgt cgcctcctat
      121 gaccagggac atgtctccga aaagccagtt gacggaagtc attttttccc ctttccccag
      181 cggggtcgga atcatctggc gtctgaccca tgtttcccca tcttcatcat aggagaagta
      241 aagaagtttg tccgcgtccg tggcgatgac gaggctgtct ccggaggctc cgggcagcat
      301 ggccgtgggg cggccttcca gctggtccgt caggtcatgg aatccggaag gagccagttc
      361 cccttcatga agcagggagc gggattcttc cagcgtcatc agcaggaggc gcgtgccttg
      421 ttccgtctcc acggttgcgg tgaatatttt ccgttctccc gcgtccgcat aggctatgcc
      481 ggatattttg ccggggacgg agccgttttc cgtcaggggg tagagagggc cggcctccgc
      541 tccggctttg tccgggccgt gggcattcgt ccggccgtgg acgttgagtc cggaatggac
      601 gtggatgagg cctaccttgc cggattccgt agcgataggg taggcggtga gggatgcatt
      661 ataggaatgg gcgcagacgg tttcgccttc cggcagggta acgggaacgg aggacaggtt
      721 cccggtagtt ttgtccagga agaagatgtt ttttccgttg ccatagacaa agggaagcgt
      781 gccggaaggg tccaggcccc aggttcccgg aaccggagag gagggcgcgg ggtgctgccg
      841 ttcttccacg tccgcgctct ggaagagagg aatcgtaacg gccagcagga agaaaagaat
      901 gccgaaaacg gctacaatga cggtgaatcc tccgccgatg atggttttgg tcatgatgcg
      961 gtcaaaccac agcgtggttt ttgccacctg aaagcgttcc ggaacgggtt tgggctttct
     1021 ggaaggtgcg ggggagggag cattggaact catgacggca gagaggagaa gttgaagggc
     1081 aggactccgg cgggtgggcc ggagtcctgc cccggcatgt gtttacggca cgggaaggga
     1141 gtgtttattc aatgctcttg agcacttctg cgctgacttt ggcgggaatc gggtaataac
     1201 cgtccttggt cacgatttcc tggccgtctt tggatacgat gaacttgatg aattccctgg
     1261 tcagggtgtc caggggttct cccggtttct tgttgacgta aatcagcagg taacgggaga
     1321 gcgggtatgt tccgttgatg cagttatcgt aggaaggctg taccgccgcc tttccgcttt
     1381 tttccgccag ggagagggtt tttacgccgg aggtgacgta tccgatgccg gaatagccga
     1441 ttccctgttc gtcggaactg atgccctgca cgacggcgga ggaaccgggc tgttccttca
     1501 cggagttttt ataatcccct tttttcaggg ctatttcctt cacgaacccg ttcgtgccgg
     1561 aggcgctgtt tctgccgtag atggagatgg cttttccttt catggagggc actcccgcat
     1621 cgccccagtc ggtgatattg gagccgccgc gcttgaaagt ggaggagaaa atggagtcga
     1681 tctgcgccag ggtaagggcc tggatgggat tgttcttgtt gacaaagaag gccacggcgt
     1741 ccagggccac tttgatttcc gtcggcttgt agccgtattt ggcttcaaag gcggcgattt
     1801 cctcccgctt catctggcgg ctcatggggg cgagctgggc cgtgcctgcc gtgagggcgg
     1861 gcggcgctgt ggaagagcct ttgccttcaa cgccgatttt gacgctggga tattttttgc
     1921 tgaatccttc cgcccagagg gtcatcaggt tgttcagcgt atcggagccg acggcgttca
     1981 ggttgccgga aacgccgctg gagggcgcat acggcttgat gctgttgtca acggtcactt
     2041 gactggagcc catgctcagg gagctgaggg cggcggcgat ggtcaggatt ttggcgacga
     2101 atttcatggc gtctttttct gatgtgttgc gtcttccgtt tctatgccgg cggggccgtt
     2161 cattgcggaa gacggggtag tgatactctc ccgcccccat ggtggggaag agaatcctgg
     2221 ttaagaaatc gtcacaaacc cggagtagcc aggaagccct gtttttggga taccatgcgt
     2281 tcagaggcgc aggtcgcgcc attagaaaaa catgactccg catccagcag cagccgacca
     2341 gaccgccgta gaggtggatt ccctgaattt ttgttatggg agcaagcaag ccctgttcaa
     2401 cgtcaccatg aagattccca agaacagggt aacggcattc atcggccctt ccgggtgcgg
     2461 gaaatccacg ctgctgcgct gtatcaaccg gatgaatgac cgtattccgg aggcgcgcgt
     2521 tacggggggc tctatccgca ttgacggggt ggacgtgact tccccttccc tggatgccat
     2581 tcgtttgcgg cgggaggtgg gtatggtttt ccagaagtcc aaccctttcc cgatgagcat
     2641 ttatgagaat gtagcctacg gattgaagct ggcgggggta aaaaaacgcg gcattctgga
     2701 cgaggctgtg gaggaaagcc tgcggcatgc cgctctttgg gatgaggtga aagaccgtct
     2761 ccatgcctcc gccaatggac tttccggcgg ccagcagcag cggctgtgca ttgcccgctc
     2821 cattgccgtg aagccgcgca tcctgctgat ggacgaaccc tgttccgccc tggaccccat
     2881 tgcgacggtg agggtggaag agctgatgca ccgtctcaag gacaagttta ccatcattgt
     2941 ggtcacccat aatatgcagc aggccacgcg cgtttcggac ctgaccggtt ttttcatgtt
     3001 gggcaggctg gtggaatttg atgctacgga aaagattttt tccaatccct ccctgaaaga
     3061 aacggaggat tacattacgg gaagattcag ttaacagaga gacgattatg atttttccaa
     3121 agaatatttc c
//
LOCUS       DAOMSU010000252         3128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3157_length_3128_cov_2.455581, whole genome shotgun sequence.
ACCESSION   DAOMSU010000252 DAOMSU010000000
VERSION     DAOMSU010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3157_length_3128_cov_2.455581"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="PK077_09105"
     CDS             complement(<1..911)
                     /locus_tag="PK077_09105"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRN24494.1"
                     /translation="MVTPAFSDCINKLGVDEKDVIPFGRNKAKISLDVLDKPAAPGKL
                     ILVSAITPTPSGEGKTTVSIGLAQGLQAIGKKVCLALRQPSMGPVFGRKGGATGGGKS
                     SLTPAEEINMHFTGDFHAITSAHNLISAIIDNAMFFHTLNIDERKVIWKRVMDMNDRS
                     LRSIIVGLNKQGFPRETGFDITPASEIMACLCLATSYKDMEERINRIVIGFTTDDKPV
                     FARELGITGSVMALLKDALMPNLVQSVEGVPCFLHGGPFANIAHGCNSVLATRMALHF
                     GDYAVTEAGFAFDLGAEKFLDIKCRQSG"
     gene            complement(1099..1698)
                     /locus_tag="PK077_09110"
     CDS             complement(1099..1698)
                     /locus_tag="PK077_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HRN24495.1"
                     /translation="MKPAALLILLFLSCPACLMADNAALAARAREQVGVTVSYNGGYQ
                     SIPYPNGDVPRETGVCTDVVIRAMRAFGLDLQKAVHEDMKAHFSKYPKIWGLKTTDRS
                     IDHRRVPNLRTFFARKGWSVPITENAADYEPGDLVTWNLSDSIPHIGIVSDRKTEEGT
                     PLIIHNVGAGTQEEDFLFSHTITGHYRPVLAEGKQKAQP"
     gene            complement(1729..>1973)
                     /locus_tag="PK077_09115"
     CDS             complement(1729..>1973)
                     /locus_tag="PK077_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HRN24496.1"
                     /translation="SHSGDAIQGLDDISNASVYHAGTKWNADKNCYETNGGRVLAVVA
                     QGDTLSEARQRAHNEIKKIQFNGMQRRPDIGFASFV"
     assembly_gap    1974..1986
                     /estimated_length=13
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1987..2994)
                     /gene="purD"
                     /locus_tag="PK077_09120"
     CDS             complement(<1987..2994)
                     /gene="purD"
                     /locus_tag="PK077_09120"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HRN24497.1"
                     /translation="MKIAVIGKGGREHALVKALKESPSAPEMYCFPGSDAINRLATPI
                     PARDLPSLIDWMVSNKMDLCVAGEESYLVKDEGLANACTRAGIPCWGPVKESAQLEAS
                     KEFAKDFLLRHRIPTGQARVAATLEEARQFIGNNYPTVLKFDGLAAGKGVAVCMSKEE
                     ADSFLKEVFTDRRFGEGRLLVEEFLTGPEVSIFGALVDDHYLILCPARDYKRLKEGDA
                     GPNTGGMGAVASRRLVEPEMLERIEKEIVAPTVAGLKKDGLPYRGFLYFGLMLTPNGP
                     KVIEYNCRFGDPECQAVMPLLSGDLASFCLHGAKGEFAPEEISFQNGWSVCCVLASKG
                     YP"
BASE COUNT          625 a          847 c          923 g          720 t
ORIGIN      
        1 ccggactggc ggcatttgat atccaggaat ttctctgcac ccaggtcaaa cgcgaatccg
       61 gcttccgtga cggcatagtc cccaaaatgc agggccatcc tggtggccag cacggagttg
      121 cagccgtggg caatgttggc aaacgggccg ccgtgcagga aacagggcac tccttccacg
      181 gattgaacca aattgggcat cagggcatct ttcagcaggg ccatgacgga accggtaatg
      241 cccagttccc ttgcgaatac gggcttgtca tccgtcgtga atccgatcac gatgcggttg
      301 atgcgttctt ccatgtcctt gtaggaagtg gcaaggcaca ggcacgccat gatttccgaa
      361 gccggggtga tatcgaagcc tgtttctcgg gggaacccct gcttgttgag ccccacgatg
      421 atggaacgca gggaacggtc gttcatgtcc atgacccgct tccatatgac tttgcgttcg
      481 tcaatgttca gcgtgtggaa gaacatggcg ttatcaatga tggcgctgat gaggttgtgg
      541 gcggatgtaa tggcgtggaa gtcccctgtg aaatgcatgt tgatttcctc tgccggcgtc
      601 agggaacttt tgccgcctcc ggtagccccg cccttgcggc cgaacaccgg ccccatggaa
      661 ggttgccgga gcgccaggca gacttttttg ccgatggcct gcaatccctg cgccaagccg
      721 atggaaacgg tggttttgcc ttctccggat ggggtgggag tgatggcgga taccaggatg
      781 agtttgccgg gggctgccgg cttgtccaat acgtccaggg aaattttggc cttgttgcgg
      841 ccgaagggga tgacgtcttt ttcatcgacg cccagcttgt tgatacagtc ggagaatgca
      901 ggtgtaacca tgcgcacatg atgacggaag ccccctcttt tggcaagaag gttcatgggc
      961 attccggacc ggatgaatca atgagcggga aagggccgcc ttcttcctgt cccgcgacat
     1021 tccccggata tccatgcaac tccgggagaa cctccggaaa tacccggtat ggaggttgcg
     1081 ctaagacggg ggtaagaatc agggttgcgc tttctgtttc ccctcagcca gcacgggacg
     1141 gtagtggccc gtgatggtat gggagaacag gaagtcttct tcctgcgttc cggcgccgac
     1201 attatgaatg atcagcggcg tgccttcctc cgttttccgg tcggaaacga tgccaatgtg
     1261 gggaatacta tcggacaggt tccatgtaac caggtcgccg ggctcgtagt ccgccgcgtt
     1321 ttccgtaatg ggaacggacc aaccttttct ggcaaagaag gtacgaagat tagggaccct
     1381 gcggtggtca atgctgcggt ccgtcgtttt caagccccat attttagggt atttggaaaa
     1441 gtgagccttc atgtcctcat gcacggcttt ttgaaggtcc aggccgaagg ccctcatggc
     1501 gcggatgacc acatccgtgc atactcccgt ttcgcgggga acgtctccgt tggggtaggg
     1561 gatggattga tagcctccgt tgtaggaaac cgttactcct acctgttcac gcgccctggc
     1621 cgccagcgcg gcgttgtccg ccatcaggca tgcagggcag gaaagaaaca ggaggatgag
     1681 aagggccgcc ggtttcatag ggcagatggg gagggaaacg cggaaaaatc agacgaagct
     1741 ggcaaagccg atgtccggcc ggcgctgcat gccgttaaat tgaatctttt tgatttcgtt
     1801 gtgggcacgc tggcgcgctt cggagagggt atctccctgt gccacgacgg ccaggacgcg
     1861 gccgccattg gtttcataac agtttttatc cgcattccat ttggtgccgg cgtggtaaac
     1921 ggaagcattt gaaatatcgt ccagtccctg aatagcgtcg ccggaatggg aggnnnnnnn
     1981 nnnnnncgga tatcctttgg aggccagcac gcaacagacg ctccagccgt tttggaagga
     2041 aatttcttcc ggcgcaaatt ctcccttggc cccatgcagg cagaaggaag ccaagtctcc
     2101 ggagagcagg ggcataacgg cctggcattc cggatcgccg aagcggcagt tgtattcaat
     2161 tacttttggg ccgtttggag tcagcatcag gccgaagtac agaaaacccc ggtaaggcag
     2221 cccgtctttt ttcagtccgg ctaccgtcgg agccacgatt tccttttcaa tgcgttccag
     2281 catttccggc tccaccagcc gacgggaagc caccgctccc atgccgcccg tgttggggcc
     2341 agcatcgcct tccttgaggc gcttgtagtc ccgtgccggg cagaggatga gataatggtc
     2401 gtccaccaga gcgccgaaga tggagacttc cggtccggta agaaattctt ccaccagcag
     2461 ccgtccttca ccaaaacgcc tgtccgtaaa tacttctttc aggaaagagt ctgcttcctc
     2521 cttgctcatg cagacggcta cgccctttcc ggcggccaga ccgtcaaatt tcagcacggt
     2581 aggatagttg tttccgatga attgccgggc ttcctccaat gtggccgcta cgcgcgcctg
     2641 cccggtaggg atgcggtggc ggagcaggaa gtccttggca aattccttgc tggcttccag
     2701 ctgggcgctt tcttttaccg gcccccagca ggggataccc gcacgggtgc aggcattggc
     2761 caggccttca tccttgacca gatagctttc ctccccggca acgcagagat ccattttatt
     2821 ggaaaccatc cagtcgatca gcgagggcag atccctggcc gggatggggg tagccagacg
     2881 gttgatggcg tcacttcccg ggaaacagta catttccgga gcggagggag attccttgag
     2941 tgccttgacc agtgcatgtt cacgtccacc ttttccaata acggctattt tcatacgccg
     3001 ccagtatgcc gggacggact gattttgcca agctgaaatg aacggtgagg ttttcccttt
     3061 tgtccggttg ttcccatgct tttcctccat tgccgggaat ggggttgtac cactttttct
     3121 tttcgtgg
//
LOCUS       DAOMSU010000253         3102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3199_length_3102_cov_2.798819, whole genome shotgun sequence.
ACCESSION   DAOMSU010000253 DAOMSU010000000
VERSION     DAOMSU010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3199_length_3102_cov_2.798819"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="PK077_09125"
     CDS             <1..1283
                     /locus_tag="PK077_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRN24498.1"
                     /translation="ASALLQEESPDLVLTDLRLGGESGMDVLKAAATLPHPPQSIMMT
                     AYGSVDAAVAAMKQGAYDFVTKPLNLDAVELVLKRALHTRNLETANQELTTRIQADSG
                     LQKLLGRSAAMERVFSIIRQVAPSKTTVLIEGESGTGKELVAQAIHSLSGRPENKFVA
                     VNCAALSPQLLESELFGHEKGSFTGAGQRRIGRFEQADGGTIFLDEIGEIDAGTQVRL
                     LRVLSERTIERVGSNASIPVNVRVIAATNKSLKKLVQEGKFREDLYFRLNVVHIQMPP
                     LRDRREDIPLLATAFLKEFARENNKEFKPLSRDALEAVRNYSWPGNVRELRTAMEHGV
                     VMSNSASIGLHHLPPQLQDTSPETIPQKRPEDNAPNTQNGLVPAGVLNLSLLERNAIQ
                     QALAQSNGNKTAAAHLLGISRRTLQRKLQDILHS"
     gene            1280..>3102
                     /locus_tag="PK077_09130"
     CDS             1280..>3102
                     /locus_tag="PK077_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24499.1"
                     /translation="MNEPSSASRTPFIIILILLLAITGVQYFFNFNGLGTDAAMDHAQ
                     IARNVARGQGMATNWLRPIQMVSGSTRAGLNPFLSDAQIHEQEAIRAGQGETLVDPEK
                     FNPYALRDTRNAPLNILTEAAVFKMAGVHKFDLWSMTGPSMIYLPDRIVAGISCMFFI
                     LSVLSCYYILLRMFDVTIACFTCLTMILSNLFLQYATSGLPQMMMLFFFAWGIHFLYT
                     ALQNKEENRAFLWPLIGSSICFSCVCLTGWIGLWPMAGFLIFAGIRFKPHGLYCIPGL
                     ITLLLFLAYPIYINRSLSGGFFGTAYYTIFTGLTGNEEIAMNALVSGSIPIAAQKVVT
                     SVINNILTQGDLLYENLGNLPLAMVFLLALLHKFKRPEVNQSKWGIFAMWVPAVIGMA
                     IYTSNKTGISLGQIQILFAPFFTAYGTAFVLNLIARHSNKELISILRGGVLLLSLLIT
                     SLPLLLNLPHIVRVGILTAGRGIPAWPPYYPLGLNRDLRTQTTEKDFILTDQPSAVGW
                     YADRKAIGIPKMVEQFMVLERILKFHGGKVGSILVTPSSTSKMNLRTISSSYGEFTPL
                     VLEGTVLFQTKDKNPVYIFDHSRALAPLAQRFGTPDSRQFI"
BASE COUNT          683 a         1000 c          751 g          668 t
ORIGIN      
        1 aggcttccgc cctcctgcag gaagaatctc cggacctggt cctgacggat ctgagactgg
       61 gcggggaaag cggtatggac gtgctgaaag ccgccgccac cctgccccac ccaccgcaaa
      121 gcatcatgat gactgcctac ggctccgtgg atgccgccgt agcagccatg aaacagggcg
      181 cgtacgactt cgtcaccaaa cccctgaatc tggatgcggt ggaactggtg cttaaacggg
      241 cgctccatac ccgcaacctg gaaacggcca accaggaact caccacccgc atccaggcgg
      301 actccggact gcaaaaactc ctgggcagat ccgccgccat ggaacgcgta ttctccatca
      361 ttcgccaggt ggcccccagc aaaacgactg tgctgattga gggggaaagc ggcaccggga
      421 aagaactcgt agcccaggcc atccattctc tctccggaag gccggagaat aaattcgtgg
      481 ccgtcaactg cgcggccctt tccccccaat tattggaaag tgagttattc ggccatgaaa
      541 aaggctcctt taccggagcc gggcaacgtc gaatcggccg ctttgagcag gcggacggcg
      601 gcaccatctt tctggatgaa atcggggaaa tagacgccgg cacgcaagtc cggctgctga
      661 gggtgctgtc tgaacggacc attgaacgcg taggctccaa tgcatccatt cccgtcaatg
      721 tgcgtgttat cgcggctacc aacaaatcat tgaaaaagct cgtgcaggaa ggaaaattcc
      781 gggaagacct ctacttccgc ctcaatgtgg tgcatatcca gatgcccccc ctccgggacc
      841 gccgtgaaga catccccctg cttgccaccg cctttctgaa agaattcgcc agggaaaaca
      901 acaaggaatt caagcccctc tcccgggacg cccttgaagc cgtccggaat tactcatggc
      961 cgggaaacgt tcgtgaactt cgcacggcca tggaacacgg cgtcgtcatg agcaattccg
     1021 ccagcatagg cctccaccat cttcccccgc aactgcagga tacttctccg gaaaccattc
     1081 cacagaaacg gccggaggac aatgcgccca acacgcagaa tgggcttgtc ccggccgggg
     1141 ttttgaattt atccttgctg gaacggaatg ccatccaaca agcgctggcc cagtcaaacg
     1201 gcaacaaaac ggcagcggcc cacctgctgg gcatcagccg gcgcactctt caacgcaaac
     1261 tacaagatat cctccattca tgaatgagcc ctcctcggca tcccgcaccc cttttatcat
     1321 tatccttatc ctgctgctgg caatcacggg ggtgcagtac ttcttcaact ttaacggcct
     1381 gggcaccgat gccgccatgg accacgccca aatcgcccgc aacgtggcca gggggcaggg
     1441 gatggccacc aactggctga ggcccatcca gatggtctcc ggaagcacac gcgccggcct
     1501 caaccctttt ctttcagacg cccagataca cgaacaggaa gccatccggg ccggacaagg
     1561 cgaaaccctc gtcgatccgg aaaaattcaa tccctatgcc ctccgggaca cacgcaacgc
     1621 tccgctgaac attctgacgg aagcagctgt cttcaagatg gccggcgtcc acaaatttga
     1681 cctgtggagc atgacgggcc catccatgat ttacctgcct gaccggattg tggcgggcat
     1741 ttcctgcatg tttttcatcc tgtccgtcct gagctgctat tacatccttc tccggatgtt
     1801 tgacgtcacc atcgcctgct tcacctgcct gacgatgata ctcagcaatc ttttcctgca
     1861 atacgctacc agcggcctcc cgcagatgat gatgctcttc ttctttgcct ggggcattca
     1921 ctttttgtac acggctctgc aaaacaagga ggaaaaccgc gcattcctgt ggcctctcat
     1981 cggcagctcc atctgttttt catgcgtctg ccttaccgga tggatcgggc tgtggcccat
     2041 ggccggtttt ctgatttttg caggcatccg gttcaaaccg cacggcctgt actgcattcc
     2101 gggccttatc actctccttc ttttcctggc ctaccccatt tacattaacc gctccctcag
     2161 cggaggcttc ttcggaacgg cttattacac catcttcacg gggctgaccg gcaatgaaga
     2221 aatagcgatg aacgccctgg tttccgggag cattcccatc gctgcccaaa aagtagtcac
     2281 ttccgtcatc aacaacattc tgacacaggg tgacctgctc tacgaaaacc tcggcaatct
     2341 ccccctggcc atggtatttc tgctggccct cctgcataaa ttcaaacgtc cggaagtcaa
     2401 ccagtccaaa tggggcattt ttgccatgtg ggtccctgcc gtcatcggca tggccattta
     2461 tacctccaat aaaaccggca tttccttagg gcaaattcaa atcctcttcg ctcccttttt
     2521 cacggcctat ggcacggcat ttgttctcaa cctgatagcc aggcattcca acaaagagct
     2581 aatctccatc cttcggggag gcgtcctgct gctctccctt ctcatcactt cccttccgct
     2641 tctgctcaac cttccgcaca ttgtgcgggt gggcatcctg acggccggcc gcggcattcc
     2701 ggcatggccg ccctactatc ccctgggcct taaccgggat cttcgcaccc agacgacgga
     2761 aaaggatttc attctcacgg accagccgtc cgccgtcggg tggtatgcag accggaaagc
     2821 catcggcatc cccaaaatgg tggaacaatt catggtgctg gaacgcattt taaaattcca
     2881 cggcggcaag gtgggcagca ttctggtgac tccttcttcc acgtcaaaaa tgaacctccg
     2941 caccatttcc tcctcctatg gagaattcac tcccctcgtt ctggaaggca cggttctgtt
     3001 ccagaccaag gataaaaacc ccgtgtacat ctttgaccac agccgggcgc ttgcccccct
     3061 ggcgcagcgc tttggaacgc ctgactcccg ccaattcatt ca
//
LOCUS       DAOMSU010000254         3099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3211_length_3099_cov_2.262155, whole genome shotgun sequence.
ACCESSION   DAOMSU010000254 DAOMSU010000000
VERSION     DAOMSU010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3211_length_3099_cov_2.262155"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="PK077_09135"
     CDS             complement(<1..856)
                     /locus_tag="PK077_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24500.1"
                     /translation="MKTVRAFLFSVMCLAPALPLLGDVPDWENEEVTGINKEAPRASV
                     FPASDKTLSLNGDWKFKFSMTPDERPADFFNPSYSVSGWDTVKVPSNWQLYGYGTAIY
                     TNVPYPFKPNPPKVMGEPPKNWPAYRERNSVGSYRRDFNLPVSWKGEQVMVRFDGVES
                     AFYVWVNGRQVGYSEDSYTGAEFDLTPHVRPGRNTIAVEVYRWSDGSYLEDQDFLRLS
                     GIFRDVTLFAQPQTHVRDLFLKAGLDREDYTTGVLDGTFTIRNSGKKDIPAGMKLNYS
                     IDGISTKLN"
     gene            complement(879..>1807)
                     /locus_tag="PK077_09140"
     CDS             complement(879..>1807)
                     /locus_tag="PK077_09140"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRN24501.1"
                     /translation="QHWNHPSICMWGIFNEIGHQPEAATRGVDMEAELTELNKFVKET
                     DPSRMTVGASNQTGRRKLNNIPDHIAFNTYPGWYGGGPETMKGNLNGYIRDYGGKMGM
                     AVSEYGHGASTGMHENPAKRPSPTGFWHPEEWQSHAHEINYRCIRERPEVWGAFVWNM
                     FDFGSSSRFEGESPGINDKGLVTYDRKTPKDAYFFYRANWSPRPTVYITSRRFAERTE
                     KTVPVKVYSNGSVVKLSVNGKPVGSVKPDDLKRAVWPEVILKPGLNTITATASIGGKT
                     YTDSCQWTLNPSGGDGKKDSERYQDPSLKKYK"
     assembly_gap    1808..1849
                     /estimated_length=42
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1850..3004)
                     /locus_tag="PK077_09145"
     CDS             complement(<1850..3004)
                     /locus_tag="PK077_09145"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HRN24502.1"
                     /translation="MNIRIHSFNVSCVGLCCVLGSFFAADAREVYPLNGSWDFSFNGK
                     PAEKVTVPHTWNAEDAANGAQGKREDAKSVNSDAYRRGPAVYSRTLPVEPRPGKRYFI
                     RGGGASIVSEVSVNGKPAGSHEGAFTAFCYEITPLLKKGSNTISVLVDNTQRNHIAPQ
                     RGDFSMFGGLYRPIELIETDGVCIDPLFYASPGVFITTKSLSKSKAEVEVKTLLNSDG
                     KAGDVDVAVEILDMKGRKVAGKTVKAAAGEKNRLEVPVTLAIANPVLWNGVKNPYLYQ
                     VKTSIRTADGQTDELVQPLGLRTVSVNPKEGFVLNGKTMQIKGVSRHQDMEGKGWALS
                     PEDEARDIRLIADMGADGVRTGHYPASTNIYDLCDKAGLIVWSEVPNVNLV"
BASE COUNT          676 a          911 c          763 g          707 t
ORIGIN      
        1 agttcaattt ggtggaaatc ccgtcgatgg aataattcag cttcatgccg gcgggaatat
       61 cctttttacc ggaattgcgg atggtgaacg ttccgtccag taccccggtt gtgtaatcct
      121 ccctgtccag tccggccttc aggaacaagt cgcgcacgtg cgtctgcggc tgggcaaaca
      181 gggtcacgtc ccggaaaatg ccggaaaggc gcaggaaatc ctggtcctcc aggtaagaac
      241 cgtcgctcca gcggtatact tccacggcaa ttgtattcct gcccggcctg acatggggag
      301 taaggtcaaa ctccgcccct gtataggaat cttcggaata gccgacttga cggccattca
      361 cccacacata aaaggcggac tccaccccgt caaaccggac catcacctgc tctcccttcc
      421 aggaaacggg caaattgaaa tcccggcggt aggaacccac ggaattacgc tccctgtacg
      481 ccggccagtt cttgggcggt tcccccatta ccttgggagg attcggcttg aatggatagg
      541 gaacattcgt gtaaatggcc gttccataac cgtaaagctg ccagttggaa ggaaccttga
      601 ccgtatccca tccggaaacg gaataggagg gattgaagaa atcggcaggc ctctcgtccg
      661 gagtcatgga aaatttgaac ttccaatccc cgttcaagct caacgtttta tctgatgccg
      721 ggaaaacgga ggctcgggga gcctccttat tgattcccgt cacctcttca ttttcccaat
      781 ccggaacgtc ccccagcagg ggaagagccg gagccagaca catcacggaa aacagaaacg
      841 cgcgtaccgt cttcatctcg gaatgaatat ggaattgctt atttatactt tttcagggac
      901 ggatcctgat aacgctctga atccttcttc ccgtcaccac cggagggatt caacgtccac
      961 tggcaggaat ccgtgtacgt tttccctcct atggaagccg tggccgtaat agtgttgagg
     1021 ccgggcttga gaatcacttc tggccagacg gcgcgtttca aatcatccgg tttgacactc
     1081 ccaacgggct tcccgttgac gctcagcttc accacggaac cattgctgta aactttcacg
     1141 ggcaccgttt tttccgtacg ttccgcaaac ctgcgggatg tgatgtatac ggtaggacgg
     1201 gggctccaat tggccctgta gaaaaaatag gcgtccttgg gcgtcttgcg gtcataggtc
     1261 accagcccct tgtcgttaat gccggggctt tccccctcaa aacgggaaga ggaaccgaag
     1321 tcaaacatgt tccagacaaa ggccccccac acctccggcc tttccctgat gcacctgtaa
     1381 ttgatctcat gcgcatggga ctgccattcc tccggatgcc agaatccggt gggagacggc
     1441 cttttggccg gattctcatg catgcccgtg ctggcgccgt gaccatactc actgacggcc
     1501 atgcccatct tgccgccata gtcgcggatg tagccgttca gattgccctt catggtttcg
     1561 ggccctcctc cgtaccagcc gggatacgta ttgaatgcga tgtggtcagg aatattgttc
     1621 agttttctgc gtcccgtctg attgctggcc cccacggtca tgcgggaggg atccgtctcc
     1681 ttcacaaact tgttcaattc cgtcaactca gcttccatgt ccactcctct agtggcagcc
     1741 tcgggctggt gtccgatttc attgaaaatt ccccacatgc aaatggaggg atgattccaa
     1801 tgctggannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc accaggttga
     1861 cattggggac ctcggaccag acaatcagcc ccgccttgtc gcacaggtcg taaatattgg
     1921 tggatgcggg atagtggccc gttctaaccc catccgcccc catatccgca atcagcctga
     1981 tatcccgcgc ctcatcttcc ggggaaaggg cccacccctt cccctccata tcctgatggc
     2041 ggctgacccc cttgatctgc atggtctttc cgttcaggac aaaaccttcc ttgggattga
     2101 cggaaaccgt cctcagcccc agcggctgca ccagctcatc cgtctggccg tccgccgtcc
     2161 tgatggaagt tttcacctgg tacaggtaag gatttttcac tccgttccag agtacgggat
     2221 tggcgattgc cagcgttacg ggaacctcca gcctattctt ttccccggcg gcggccttta
     2281 ccgtcttccc ggctaccttc ctgcccttca tgtccaggat ttccacggca acatccacat
     2341 ccccggcctt tccgtccgaa ttgagaaggg tcttcacttc cacctcggcc ttggatttgc
     2401 ttaaagactt tgtggtgatg aaaacgccgg gagaagcata aaaaagagga tcaatgcaga
     2461 caccgtccgt ctcaatcagt tcaatgggcc ggtacagccc gccgaacatg gaaaaatccc
     2521 cgcgctgggg ggcgatatga ttacgctgcg tgttgtccac cagaacggaa atcgtattgg
     2581 agcctttttt caaaaggggc gttatttcat aacagaaagc cgtaaacgcc ccctcatggc
     2641 tgccggcagg cttcccattg acggaaacct cggaaacaat gctggctccc ccgccgcgga
     2701 tgaaatacct cttgcccggc ctgggctcca ccggaagcgt gcgcgaataa acggccggcc
     2761 cgcggcggta ggcgtcactg ttgacggatt tggcgtcttc ccgcttcccc tgagcaccat
     2821 tggcggcatc ctctgcgttc catgtatggg gcaccgtcac tttttcagca ggcttcccgt
     2881 taaaactgaa atcccaggaa ccgttcaggg gataaacctc acgggcgtcc gccgcgaaaa
     2941 aagaccccaa tacgcagcaa aggccgacac atgatacgtt gaaagaatga attctgatgt
     3001 tcatggtaca gaaaagcagt acctaccata tacgttcagc catataaaat actttcatcc
     3061 ggccgttcct ttacccctct tccgacagaa atatactca
//
LOCUS       DAOMSU010000255         3084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3234_length_3084_cov_2.385606, whole genome shotgun sequence.
ACCESSION   DAOMSU010000255 DAOMSU010000000
VERSION     DAOMSU010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3234_length_3084_cov_2.385606"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PK077_09150"
     CDS             complement(<1..516)
                     /locus_tag="PK077_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRN24503.1"
                     /translation="MDTREFNEIIAANSSWISALRTEVGKVVIGQQALVDRLILSLLC
                     KGHVLLEGVPGLAKTLSVKAMAGTLHAQFARIQFTPDLLPADLLGTMIYNPEERQFTA
                     KKGPIFANLILADEINRAPAKVQSALLEAMQERQVTLGETTYRLPDPFLVLATQNPID
                     QEGTYQLPEAQL"
     gene            complement(571..1983)
                     /gene="gatA"
                     /locus_tag="PK077_09155"
     CDS             complement(571..1983)
                     /gene="gatA"
                     /locus_tag="PK077_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRN24504.1"
                     /translation="MSKIQGTLAQWRDRLRRKELSPAELVNLMADAIEADRTTNAYIS
                     FDREAALHAAAGADISSPLAGIPIAVKDNINVLGQPTRCASRLLSPYVAPYDATSIRL
                     LKEAGGIPLGRTNMDEFAMGASGENSAYGITRNPEAPDRIPGGSSSGSAAAVASATAV
                     AALGSDTGGSIRQPAGHCGIVGLKPTYGRVSRYGLVAFASSLDQIGPMTRTVEDAAIL
                     LQAISGHDRKDSTSANCPVPDFEAALGRDVKGLKVGIPSEYFTSGNHPGISEAVQNTV
                     KQLESLGAELVEVNLPHADAVVAAYYIIACAEASSNLSRFDGVRYGKRAEDAAGLVEL
                     FSRTREEGFGPEVKRRIILGTYVLSSGYYDAYYSRAQKVRSLVARDFAEAFSRADIIV
                     GPTSPAPAPKIGDSALDHLQTYLADIYTIPANLAGLPAMSIPCGTVRESGMELPVGFQ
                     MMAPHFREDLLLKTGFALGK"
     gene            complement(2038..2331)
                     /gene="gatC"
                     /locus_tag="PK077_09160"
     CDS             complement(2038..2331)
                     /gene="gatC"
                     /locus_tag="PK077_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRN24505.1"
                     /translation="MSTPQIDVAYIAKLARIDLTEEETALFSKDLDKILAYITKLESY
                     DVTGIAPMNHPLPAMDVMREDIPETGFTQEEALSNAPQQSQGQFRTPKVVESA"
     gene            complement(2372..2704)
                     /locus_tag="PK077_09165"
     CDS             complement(2372..2704)
                     /locus_tag="PK077_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24506.1"
                     /translation="MIKRKTKRRSSELAELDRASREIESRIQELEASLKRPSQQTRMR
                     LDRNTMPPPDRVREGNQNRALRAVVARDGRATNMRRELRENFMLLGLLICAIAASFCW
                     IVRLLEQQ"
     gene            complement(2701..>3084)
                     /locus_tag="PK077_09170"
     CDS             complement(2701..>3084)
                     /locus_tag="PK077_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24507.1"
                     /translation="DLIGREVESLRQESNELEQIHMALTRSLKMLSTVRPDEWPIENT
                     DNLISQGQQVIDRCEEEFADAVQQCARMQHTKVLTGVRRSSKVRISSREFMTQFLQGL
                     SFHLPLLLIALIILFFIWIFTPSAS"
BASE COUNT          587 a          781 c         1060 g          656 t
ORIGIN      
        1 aagctgggct tccgggagct ggtaggtgcc ctcctggtca atcgggtttt gcgtggcaag
       61 caccaggaag gggtccggca gacggtaggt ggtttcaccc agggttacct ggcgttcctg
      121 catggcttcc agcagggcgg attgcacttt ggccggggcg cggttgattt catccgccaa
      181 aatgagattg gcgaagatcg ggcctttttt ggctgtgaac tgcctttctt ctggattata
      241 gatcattgtg cccagcaaat ccgccggaag aagatccggc gtaaactgga tgcgggcaaa
      301 ctgtgcatgc agcgtgcccg ccatggcttt tacggagagc gtcttggcaa ggccgggcac
      361 gccttccagc aggacgtggc ccttgcagag gagactgagg attagtctgt ccacgagcgc
      421 ctgctggccg atgaccacct tgccgacttc cgttctgaga gcagaaatcc acgatgagtt
      481 cgcggcgata atttcgttga attctcgggt gtccatgatt ctgatagcgg gtgagacagt
      541 atgccgggca cttacgttga aaaaatccta ttatttccca agggcaaatc cggttttcaa
      601 caggaggtct tcccggaaat ggggagccat catctgaaag cccacgggaa gttccatgcc
      661 gctttccctg acggttccgc agggaatgga catggcgggg aggccggcca ggttggcggg
      721 gatcgtgtag atgtccgcca gataggtctg gaggtgatcc agcgcggaat ctccgatttt
      781 gggagcggga gcgggagagg tggggcctac gatgatatcc gccctggaaa aggcttccgc
      841 gaaatctctg gcgaccaggg agcggacttt ttgagcgcgg gagtagtaag cgtcatagta
      901 accggagctg agcacgtagg tgcccaggat gatgcggcgc ttgacttccg gcccgaatcc
      961 ctcttcacgg gtgcgtgaga agagttccac cagtccggcg gcgtcttccg ccctctttcc
     1021 gtagcgcacg ccgtcaaacc gggagaggtt ggaagacgct tccgcgcagg cgatgatgta
     1081 gtaggcggcg acgacggcat ctgcgtgggg caggttgact tccaccagct ccgcgcccag
     1141 gctttccagc tgtttgacgg tgttttgcac agcctcggaa atgcccggat ggttgccgga
     1201 ggtaaagtat tccgaaggga tgcccacttt cagccctttg acgtcacggc ccagcgcagc
     1261 ttcaaagtcc ggcacggggc agtttgcgga tgtggagtct ttacggtcgt gtccggagat
     1321 ggcctggagc aggatggcgg cgtcttccac tgtccgggtc atggggccga tctggtccag
     1381 ggaagaggca aaggctacca gtccgtagcg ggaaacgcgc ccgtaggtag gcttgaggcc
     1441 cacgataccg cagtgcccgg ccggttgccg gatggagccg cccgtgtcgg aaccgagtgc
     1501 ggcaacggcg gtagcggatg cgacggcggc ggcagagccg ctggaggagc cgccgggaat
     1561 gcggtccggg gcttccgggt tgcgcgtgat gccgtaagcg gagttttctc cgctggcacc
     1621 catggcgaat tcatccatgt tcgtgcggcc tagcgggatg ccgcccgcct ccttcagcag
     1681 gcggatggag gtggcgtcat aaggagcgac ataaggggaa agaaggcggg aggcgcagcg
     1741 cgtcggctgg cccaggacat tgatgttgtc cttcacggcg atggggatgc cggccagggg
     1801 gctggaaata tccgctccgg ccgcggcgtg gagggctgct tcgcggtcaa aggagatgta
     1861 ggcgttggtt gtccggtccg cctcaatggc gtccgccatc aggttgacca gttcggcagg
     1921 ggagagttcc ttgcggcgca ggcggtcacg ccactgggcc agggtgcctt gaatttttga
     1981 catgagaagg aaagggtaaa gacggttgaa aggtgaaggg aaaaggaagc gcgaggatca
     2041 ggcggattcc accaccttgg gtgtgcggaa ttgcccctgg gactgctgag gggcattgga
     2101 aagggcttct tcctgggtga agccggtttc aggaatgtct tcgcgcatca catccattgc
     2161 cgggagagga tggttcatgg gggcgatgcc cgtgacatcg taggattcca gcttggtgat
     2221 ataggccagg attttgtcca gatctttgga gaagagagct gtttcttcct ccgtcaggtc
     2281 aatgcgtgcc agcttggcaa tgtaggctac gtctatttgg ggcgtgctca tggtgtaatg
     2341 tagaaaagat gcggaaaaga aggggggctg cctactgttg ttccagcagg cggacgatcc
     2401 agcagaagga agcggcgatg gcgcagatca gcaggcccag cagcatgaag ttttctcgca
     2461 gttcccgcct catgttggtg gcgcgtccgt cacgggccac tacggcacgc agtgcccggt
     2521 tctggttgcc ctcccgaacg cggtcgggag gcggcatggt attgcggtcc aggcgcatgc
     2581 gggtctgctg ggacgggcgt ttgagagagg cttccaattc ctggatacgg ctctcaattt
     2641 cccgtgaggc acgatccagt tctgccagtt cggaggaacg gcgtttggtt tttcttttaa
     2701 tcatgatgcg gaaggggtga aaatccagat gaaaaagagg atgatgaggg caatcaggag
     2761 caggggaaga tggaaggaaa ggccctggag gaactgggtc atgaattctc gggagctgat
     2821 gcgcactttg ctggagcggc gcaccccggt gagcactttg gtatgctgca tgcgcgcgca
     2881 ctgctggacg gcatccgcga attcttcctc gcagcggtca atgacctgct ggccctggct
     2941 gatgaggttg tccgtatttt caataggcca ttcatccggg cgcacggtgc tgagcatttt
     3001 caggctgcgc gtcagcgcca tgtggatttg ttccagttca ttgctttcct ggcggaggct
     3061 ttccacttcc cggccgatga ggtc
//
LOCUS       DAOMSU010000256         3061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3260_length_3061_cov_2.745509, whole genome shotgun sequence.
ACCESSION   DAOMSU010000256 DAOMSU010000000
VERSION     DAOMSU010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3260_length_3061_cov_2.745509"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PK077_09175"
     CDS             <1..618
                     /locus_tag="PK077_09175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24508.1"
                     /translation="ACSLARQMGPWRTEGLDKRLERGMPVRKREVLEAMMARNIHCPS
                     SSSMGRLFDAVAFWCGFDGAAGCEGHAAMMLESWAAEASGEKMPGDAYEWVMHEEEGL
                     LELDWRPLMKSVDEDLLEGVSRGCIARKFHESLVNLVFDVAERFCLDRLVLGGGCFQN
                     AFLLEGLAGMAQSRRCQLALPQRVPCNDGGISLGQVAAVVRQWKG"
     gene            622..864
                     /locus_tag="PK077_09180"
     CDS             622..864
                     /locus_tag="PK077_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HRN24509.1"
                     /translation="MCLAVPGKIVSVNETDPLFRLGVVDFGGVTREVNLACVPEAVPG
                     DYVIVHVGMALSVLDEETALQTRREMREIVENTDPL"
     gene            898..1965
                     /gene="hypD"
                     /locus_tag="PK077_09185"
     CDS             898..1965
                     /gene="hypD"
                     /locus_tag="PK077_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HRN24510.1"
                     /translation="MQDEVRQLIEELRHAVTRPWAVMEVCGGQTHAIASLGLEELLPP
                     GLRLIHGPGCPVCVTAVELIDQAVELSLRPGVVLCSYGDMMRVPGSRGDLFSAKAGGG
                     NVLLVYSPLEAVAYAGSHPDTEVVFFAVGFETTAPATALALQQARALGYANFSVLCAH
                     VLVPPSLEWLMDQDEGRPDAFLAPGHVCAVTGERDYGRLASCYRTPMVVTGFEAPDLL
                     RGILMCVRQLEAGEYAVQNAYGRYVKPGGNRAAQERMNEVFEPEDRHWRGLGLIPGGG
                     MRLRREWNDMDAVLRFECGTGRQGADEASGCLAGQVLRGLIRPGECPFFGSSCTPLAP
                     LGAPMVSGEGACAAYYHYKRS"
     gene            1970..3004
                     /gene="hypE"
                     /locus_tag="PK077_09190"
     CDS             1970..3004
                     /gene="hypE"
                     /locus_tag="PK077_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HRN24511.1"
                     /translation="MFECPVPEPVSDRIQMAHGGGGRLMNELIRTVFLSAFGCPSGGV
                     QNDAAVLEIPPGRLAMTTDSFVVQPLEFPGGSIGSLAVHGTVNDLAMSGAEPLYLTAG
                     FILEEGLPLEVLARVAQDMAAAARAAGVRIVTGDTKVVERGKGDGIYINTAGVGIVRH
                     GLEISPSSVRPGDSVLLSGDLGRHGMTIMSLRAGLSFGDGLESDSAPLHESVAAVIRA
                     GIPVHCLRDVTRGGLTATLSEIAESAGLTVKLNEMSIPVREDVRAACGLLGLDPLQVA
                     CEGRYLAVLPREHEEEALTLMCGCGVSAGACVIGRVEELGTAPLLMTGLLGVERVLTM
                     PSGMQLPRIC"
BASE COUNT          499 a          737 c         1118 g          707 t
ORIGIN      
        1 gcctgttccc tggcccgcca gatggggccg tggcgtacgg aggggcttga taagcgtctg
       61 gagaggggga tgcctgtgcg gaaacgcgag gttctggagg cgatgatggc ccggaatatc
      121 cattgtccgt cctcctcgtc catggggcgg ttgtttgatg ccgtggcttt ctggtgcggt
      181 tttgacggtg cggcggggtg tgagggccat gccgccatga tgctggaatc atgggccgcg
      241 gaagcttccg gggaaaaaat gccgggggac gcgtatgaat gggtcatgca tgaagaagag
      301 ggattgctgg aactggattg gcggccgttg atgaagtctg tggatgaaga tttgttggaa
      361 ggcgtttccc gcggctgcat cgcccgcaag tttcatgaga gcctggtgaa tctggtcttt
      421 gacgtggcgg agcgtttctg cctggatagg ctggtgctgg gcggcggatg ttttcagaat
      481 gcctttttgc ttgaaggact ggcgggaatg gcccagtcta gaaggtgcca gttggctctg
      541 ccccagcggg tgccgtgcaa tgacggggga atttccttgg ggcaggttgc ggcggtcgta
      601 cgccaatgga aaggatagaa tatgtgtttg gccgttcccg gaaagattgt gagcgtcaat
      661 gagacggacc ctttgttcag attgggggtg gtggactttg gcggcgtgac ccgtgaagtg
      721 aacctggcct gtgtgccgga agctgtgccg ggggattacg tcatcgttca cgtaggcatg
      781 gctctgagtg tgctggatga ggaaaccgct cttcaaaccc gcagggaaat gagagaaatt
      841 gtggaaaata cggatccgct gtagggaggg gcgttttccg aattcaatta atgatttatg
      901 caggatgagg tccggcagct gattgaagaa ttgcgccacg ctgtgacgcg tccgtgggcg
      961 gtcatggagg tgtgcggagg ccagacgcat gcgattgcct ctctggggct ggaagagctg
     1021 ctgcctcccg gcctgcgcct gattcacggt cccggctgcc cggtctgcgt gacggcggtg
     1081 gagttgattg accaggccgt ggaattgagc ctgcggcccg gcgtggtttt gtgcagctac
     1141 ggggatatga tgcgggtgcc gggttcccgc ggggatttgt tttccgccaa ggccggtggg
     1201 ggaaacgtgc ttctggtata ttctccgctg gaagcggtcg cttatgccgg aagccatccg
     1261 gatacggaag tggtgttttt cgccgtaggt tttgagacga cggccccggc tacggctctt
     1321 gccctgcagc aggcccgcgc actggggtat gccaattttt ccgttctttg cgcccatgtg
     1381 ctggttcccc cgtctctgga atggctgatg gatcaggatg agggaaggcc ggacgctttc
     1441 ttggcccccg gccatgtctg cgccgtcacg ggggaaaggg attacgggcg tttggcctcc
     1501 tgttaccgga cgcccatggt ggtgacgggg tttgaggctc cggacctgct ccgcggcatt
     1561 ctgatgtgcg tccgccagct ggaagccggg gaatatgcag tgcagaatgc gtacggacgt
     1621 tatgtgaagc ctggtgggaa cagggcggcg caggaaagga tgaatgaagt ttttgagccg
     1681 gaagaccgcc attggcgcgg tctggggctg attcccggcg gcggcatgag gctgcgccgt
     1741 gagtggaatg acatggatgc cgttctgcgt tttgaatgcg gaacggggag gcagggagca
     1801 gatgaagctt ccggatgcct ggccgggcag gtgctgaggg ggctgattcg tcctggagag
     1861 tgcccttttt tcggctcgtc ctgcacgccg ctggccccgc tgggggctcc catggtgtcc
     1921 ggggaagggg cttgcgccgc ttattatcat tataaaagaa gttgatgcca tgtttgagtg
     1981 tcctgttccg gagcctgttt ccgaccgtat ccagatggct cacggcggcg gaggccgctt
     2041 gatgaatgag ctgatccgaa ccgtgtttct gagcgccttc gggtgccctt ccggaggcgt
     2101 gcagaatgac gccgccgtat tggagattcc tccggggagg ctggcgatga cgacggacag
     2161 ctttgtggtg cagcctttgg agtttcccgg cggttccatc ggttcactgg cggtgcacgg
     2221 aacggtgaat gatctcgcca tgagcggcgc ggagccgttg tatttgacgg cgggctttat
     2281 tctggaagaa gggcttccgc tggaagttct ggcccgcgtg gcgcaggata tggctgccgc
     2341 ggcccgtgcg gcgggcgtcc gtattgtgac gggggataca aaagtggtgg agcgcggaaa
     2401 gggggacggc atttacatta atactgccgg ggttggcatc gtgcgccatg ggttggagat
     2461 cagcccttct tccgttcgtc cgggggattc cgtgctgctc agcggggatt tggggaggca
     2521 cggcatgacg attatgagcc tgcgcgccgg gctgtctttc ggagacggcc tggaaagtga
     2581 ttccgctccg ttgcatgaat ccgtggccgc cgtcattcgt gccggcattc ccgtgcattg
     2641 cctgcgtgac gtgacccgcg gcggattgac cgccactctt tcggagattg cggaatctgc
     2701 cggcctgaca gtgaagctga atgaaatgtc cattcccgtg cgtgaggatg tcagggcggc
     2761 gtgcgggctg ttggggctgg accctcttca agtggcctgt gagggacgtt atctggctgt
     2821 tctcccacgg gagcatgagg aagaggccct gaccctgatg tgcggctgcg gcgtatctgc
     2881 cggagcctgc gtcataggcc gggtggagga attgggaacg gcgcccctgc tgatgacggg
     2941 acttcttgga gtggagcggg tgttgacgat gccttcagga atgcagcttc cccgcatctg
     3001 ctgattcctg gtgctgcatg attggcgttt tgtggcaggg aagcgggggg aattcgcttc
     3061 c
//
LOCUS       DAOMSU010000257         3054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3270_length_3054_cov_2.221407, whole genome shotgun sequence.
ACCESSION   DAOMSU010000257 DAOMSU010000000
VERSION     DAOMSU010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3270_length_3054_cov_2.221407"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="PK077_09195"
     CDS             complement(<1..818)
                     /locus_tag="PK077_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24512.1"
                     /translation="MSRSLTFSFDIGYASIGWAVIESASPDDADPSVCGCGAVVFPKD
                     DCQAFKRREYRRLRRNIRSRRVRIERIGRLLVQAQIITQEMKETPGHPAPFYLASEAL
                     KGHRNLAPIELWHVLRWYAHNRGYDNNVSWSGGFSEDGGNKEDTERVQHAKDLMDKHG
                     TATMAETICRELKLEEGKADAPMNVSTPAYKNLNTAFPRLTVEKEVRRILELSAPLIP
                     GLTSEIMELIAQHHPLTTEQRGVLLQYGIKLARRYRGSLLFGQLIPRFDNRIISR"
     gene            1183..2289
                     /gene="alr"
                     /locus_tag="PK077_09200"
     CDS             1183..2289
                     /gene="alr"
                     /locus_tag="PK077_09200"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRN24513.1"
                     /translation="MPATSPPRAWAEIDLGAIRHNLNVVKQAAKGEYYMPVVKAGAYG
                     HGLEQVCRTLDSEGIAFFGVANVGEARRISQAGCRTRPYILGPAFPEEREEIVLNGWR
                     SFISTMEEAAHYNSLARLYGKTLPIHLSVDTGMGRGGFLPDQLEELLSRLGELDSLHL
                     EGLGAHLPCADEDREITLRQISRFEQMAARIREKLPLKYCHLANSAASLDYEIPSNNM
                     CRPGLVLYGFSPIPSPWAAQLKPAMALFSRLTVVRTLPEGHGISYGGTFVTDHPTRVA
                     TVGIGYADGYLRSLAHKGARVMVDGVSCPLLGRVTMDQIIVDVSRAPHAEPGMTAEIM
                     GPHIPVTELAEKAGTISWEIFTGIGPRVPRHYTH"
     gene            2386..2829
                     /locus_tag="PK077_09205"
     CDS             2386..2829
                     /locus_tag="PK077_09205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HRN24514.1"
                     /translation="MMKVSTRGRYALRLMIDLAQHRDEGYISLKEISARQDVTVRYLE
                     QIIAILLKAGFVQSFRGKAGGYRLSRHPREYTTEDILKLTEGSLLPLSCTASHENPCP
                     RAAGCATLPFWRGLQQVIENYLRRVTLEDLAEQQKEIGCDYGAGI"
BASE COUNT          715 a          906 c          765 g          668 t
ORIGIN      
        1 cggctgatga tgcggttatc aaaacggggg attaactgac cgaacaaaag actcccacga
       61 taacgtcggg ccaattttat cccgtactga agcaacacgc cgcgttgttc cgttgtcaga
      121 ggatgatgct gcgcaatcaa ctccatgatt tccgaagtca gccccggaat cagaggcgcg
      181 gaaagctcca atatacgtcg tacttccttt tccacggtta agcggggaaa agcggtattg
      241 agatttttat aagccggcgt tgaaacattc atcggagcat ccgctttgcc ctcttccagt
      301 ttcaattccc ggcaaatggt ttccgccata gtcgccgtcc catgtttatc catcaaatct
      361 ttagcatgct gcactctctc ggtatcttcc ttgttccctc catcttctga aaagccgcca
      421 gaccatgaga cattattgtc gtacccccgg ttatgagcat accagcggag gacatgccaa
      481 agttcaatcg gggcaagatt cctatgtcct tttaatgctt ctgacgccaa ataaaaggga
      541 gcgggatgcc cgggagtttc cttcatttcc tgcgtgatga tttgagcctg aaccagcaat
      601 ctgccgatac gctcgatacg aacgcgccga gaccggatat tgcgtctcaa acgcctgtat
      661 tcacgccttt taaatgcctg acaatcatct ttcggaaaca caactgcacc gcaaccgcaa
      721 acagagggat ccgcatcatc aggagaagcg gattcaatta cagcccaccc aatggaggcg
      781 taaccaatat cgaacgaaaa agtaagagaa cgcgacatca caaaaaactg cttttattca
      841 tgttatatgc caatattgca ttttatcaag tattttataa aaattattca attcaaaaaa
      901 acagttgaca aaacatctga acatcacttt aatttccaac ggattcaaga caaaatttga
      961 aatgcaaacc gattttcctg actgccagcc agtcacaccg gtaacaaaag catttttatc
     1021 aaaccgccat gcgtttcagc gcaaggcggt ttttattatc ttttgaaagt tcaaaagact
     1081 gacaaaaatc cgtctggaat tgcgcggcca aagacggaca atcccctgca ccgggagcat
     1141 ccggcgcccc tctttttcca acctttccgc cattttccaa ccatgccagc aaccagccct
     1201 ccccgcgcct gggccgaaat cgacctcggg gccatccgcc ataacctgaa tgtcgtcaaa
     1261 caggcggcca aaggggaata ttacatgccc gtcgtgaagg ccggcgccta tggccacggc
     1321 ctggaacagg tttgccgcac gctggattcg gaaggcatcg ctttcttcgg ggtggccaat
     1381 gtgggagaag cccggcgcat cagccaggcg ggctgccgca cccgccctta catcctgggc
     1441 cccgcgttcc cggaggaacg ggaggaaatc gtgctgaacg gctggcgctc cttcatttcc
     1501 accatggagg aagccgcgca ttacaattcc ctggcgcggc tgtacggaaa gacgctgccc
     1561 atccaccttt ccgtggatac gggaatgggc cgcggcggat tcctgccgga ccagcttgaa
     1621 gaactgcttt cccgcctggg agaactggac agcctgcatc tggaaggcct gggcgctcac
     1681 cttccctgtg cggacgagga ccgggagatt acgctcaggc aaatctcccg ctttgaacag
     1741 atggccgccc gcatccggga aaaactgcct ttgaagtact gccacctggc caacagcgcc
     1801 gcctccctgg attatgaaat tccctccaac aacatgtgcc gccccggcct ggtgctgtac
     1861 ggtttttccc ccattccctc cccatgggcc gcgcaattga agccggccat ggcccttttt
     1921 tcccgcctga cggtggtgcg cacgctgccg gaaggccacg gcatttccta cgggggcacc
     1981 tttgtgacag accaccccac cagggtggcg accgtaggca taggctatgc ggacggctac
     2041 ctgcgttccc tggcccacaa gggcgcccgc gtcatggtgg acggcgtttc ctgccccctt
     2101 ctcgggcgcg tgacgatgga tcaaatcatc gtggacgtca gccgggcgcc gcacgcggaa
     2161 ccgggcatga ccgcagaaat catggggccg catatccccg tgacggaact ggcggaaaag
     2221 gccggcacca tttcctggga gatttttacc ggaataggcc cccgggtacc ccgccactac
     2281 acccactaaa ccaggcagga gggaaaaggg ccaagccggc cgcccatttc tgggggcttc
     2341 tttcccgatt gacggacaga cagaaatatc gtttagttag ccagtatgat gaaggtttcc
     2401 accagaggac gttacgcatt gcgcctgatg attgacctcg cccagcaccg cgacgagggt
     2461 tacatctccc tgaaggagat ttccgccagg caggacgtta ccgtacgtta tctggaacag
     2521 attatcgcca tccttttaaa agcgggattc gtccagagct tccggggaaa ggccggcgga
     2581 taccgccttt ccaggcatcc gcgcgaatat actacggaag atatcctgaa actgacggaa
     2641 ggctccctcc tgcccctttc ctgcacggct tcccacgaaa atccgtgccc gcgggcggcc
     2701 ggctgcgcca cgctgccttt ctggcgcggg ctccagcaag tcattgagaa ttatttgcgc
     2761 cgcgtaacgc tggaagatct ggccgaacag cagaaggaaa tcggctgcga ctacggagca
     2821 ggcatttaag cagcggccgc gggcctgaac atttcccctt cctttctcct tccggcccat
     2881 gtcccgtttt ttttacccgg agcttgacat ggtcttttct tttcgttatc catttcctag
     2941 ttttccgata ggaatataaa aacatgagca agaaccatcg atttgaaaca cgccagatcc
     3001 atgtgggtca ggaaagtccg gacccggcca ccgatgcgcg cgccgtacct attt
//
LOCUS       DAOMSU010000258         3052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3271_length_3052_cov_2.448115, whole genome shotgun sequence.
ACCESSION   DAOMSU010000258 DAOMSU010000000
VERSION     DAOMSU010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3271_length_3052_cov_2.448115"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="PK077_09210"
     CDS             <1..1729
                     /locus_tag="PK077_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRN24515.1"
                     /translation="NSASYRWALHKIFTDNGISYRAEGVKTGNDSGGVTAGTSYGGQI
                     FNNEHSSQASARAWEISGRSNGSRFDGSNINNWLGLSGTKANGSAYSGQTFTGDNTPD
                     TFFMMIGTNDLLSDGNNATLANRLDSVSQNLLNDMDTIVEAMFQANEHANVIILTIPC
                     WTRHSNGNSDATHQAVADYNDRLKTWGNGRQNVTVIDVNAGIIDVTSSTPFYGVSSMF
                     NNPGSDGLHPNAQGDLLMAGNIARAMGYAGRTAGQERKAAGGLAINFHQGGQSPAWSS
                     NSQLENKGFSLANVTVDENGISLGQSGESSISYSWAEGTDLQNGFTFDCNLVLGNGAA
                     DGWDTASDFSISLGNSSFYGTLNINEAYIKWGDAILYSLDMSANAENLRMAYIRGNEL
                     EGLKGGYYVWLGDMLIGEALSVTSGSGCNGLTIQYNGSGTAVLHDLALDGTGSYAPAT
                     SGTLNAEKSFISSGSFTQSGTPEGNIEWKTEGFTKTADNLAGSGTFNARAQADSSAGG
                     TGNSVNVSITSGNATHIYANSGNYTGDVWLTISKEGQASAELPARSTGTSSSVSRMPP
                     QAEARSSVP"
     gene            1726..2856
                     /locus_tag="PK077_09215"
     CDS             1726..2856
                     /locus_tag="PK077_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24516.1"
                     /translation="MNAAGVTGNVYLEFSAENASFGTFTSSNSSSVVGSYATDIQGNV
                     DIVVNSGTFNHQIMGGIFANARIGTTTIGGNTHVYINGGSVTGNVMGGGLTGSISGGA
                     NVTVTGGVISGSVYGAGQGGSILAGSSVCLTGGLVKGDVYAGGKAGSIQGDTSVTITG
                     NTATLYNGSSWGSISGGGSGGTVDGNSTVRIQNLSSGTTAYGFDKYAGNISGGTNVSG
                     DRSLVLDYVTVDSLQASLSNFTHVSAVNQTRTSLDSLGGALTVTIEAGSSLILNGTSN
                     LTTLILGEHASLTLQGLTADAVVVDITGTSNYTLSLTEIPASLDNIKFLNDGVLYDAA
                     MSMDLQANSAMLFAQVPEPGSAALALAGLAPLLWRRRRKMSH"
BASE COUNT          699 a          921 c          843 g          589 t
ORIGIN      
        1 caacagcgcc tcctaccgtt gggccctcca taaaatattt acagacaacg gcatttccta
       61 ccgggcggaa ggcgtcaaaa ccggcaatga ttccggggga gttactgcgg gcacttccta
      121 cgggggccag atattcaaca atgaacattc ctctcaggca agcgccaggg catgggaaat
      181 atccggcagg agcaacggaa gcaggtttga cggctccaac attaacaact ggctggggct
      241 ttccggcacc aaggcaaacg gaagcgccta ttcaggccag acattcaccg gagacaatac
      301 gccggatacc tttttcatga tgatcggcac caacgatctc ctttctgacg gaaacaatgc
      361 cacgctggcg aaccgccttg actccgtttc ccaaaacctc ctgaacgata tggacaccat
      421 cgtggaggcc atgttccagg ccaatgaaca tgcgaatgtc atcatcctta ccattccctg
      481 ctggacccgg cactccaacg gcaattcgga cgccacccac caggccgtgg ctgattacaa
      541 tgatcgtctg aaaacatggg ggaatggcag gcagaacgtc acggtcattg acgtcaatgc
      601 cggcatcatt gacgtaacat cctccacgcc gttctacggc gtaagctcca tgttcaacaa
      661 ccccggcagc gacggcctcc atcccaatgc ccagggagat ctgcttatgg ccggcaatat
      721 cgccagggcc atgggctacg cggggcgcac cgccggacag gaaagaaaag cagctggcgg
      781 cctggccatc aattttcacc agggcgggca atcccccgca tggagctcga acagccagtt
      841 ggaaaataaa gggttttccc tcgccaatgt cactgtggac gagaacggca tcagcctggg
      901 gcaatccggg gaaagctcca tttcctattc ctgggcggaa gggacggatc tgcaaaacgg
      961 ctttaccttt gattgcaacc tggttctggg aaacggcgca gcagacggat gggatactgc
     1021 ctccgatttc agcatcagcc tggggaacag ctccttttac ggcacgctca atattaatga
     1081 ggcctacatc aaatggggcg atgccattct gtattccctg gacatgtccg ccaatgcgga
     1141 aaacctgcgt atggcatata tccgcggcaa tgagctggaa gggctgaaag gcggctatta
     1201 cgtctggctg ggggacatgc tgattgggga agcgctttcc gttacctccg gctccggctg
     1261 caacggcctc accattcaat acaacggcag cggaaccgct gttctgcacg acctggccct
     1321 ggacggcacg ggttcctatg cccctgccac ttccggaacg cttaacgcgg aaaagtcttt
     1381 tatttcttcc ggctccttca cccagagtgg aacaccggaa ggcaatattg agtggaaaac
     1441 ggagggattc accaaaaccg ctgataatct ggccggctcc ggtaccttta atgcccgcgc
     1501 ccaggcggat tcctccgcag ggggaacggg gaattccgta aacgtctcca ttacctccgg
     1561 aaacgccact catatttatg cgaacagcgg caactacacg ggggacgtct ggctgaccat
     1621 ctccaaggag ggacaggcct ccgcggagct tccggcacgc tcaacgggaa cgtcttcctc
     1681 cgtttcacgg atgccgccac aggcggaagc acggtcttcg gtgccgtgaa cgccgccggc
     1741 gtcaccggaa acgtttatct ggaattctct gcggaaaacg cctccttcgg caccttcacc
     1801 agcagtaatt cctcctctgt ggtcggctcc tatgccacgg atatccaggg gaatgtagac
     1861 attgtggtca actccggcac cttcaaccat caaatcatgg gcggcatctt cgccaatgcc
     1921 aggatcggaa ccaccaccat tggaggaaac acgcatgttt acatcaatgg cggctccgtc
     1981 accggaaatg ttatgggcgg cggcctgaca ggctccattt ccggcggcgc caacgtcacc
     2041 gtcacaggcg gcgttatcag cggttccgtt tacggcgcgg gccagggagg ctctatcctg
     2101 gcaggaagct cggtttgctt gacaggaggc ctggttaaag gggacgttta cgccggtggc
     2161 aaggcgggtt ccattcaggg ggacacgtcc gtcaccatca ccggaaacac ggccacgctt
     2221 tataacggga gctcctgggg cagcatctcc ggcggcgggt ccggcggaac ggtggatgga
     2281 aactccaccg tacgcatcca gaatctttcc tcaggaacga cggcctacgg ctttgacaag
     2341 tacgccggaa acatcagcgg cggaaccaat gtaagcggag acaggagcct ggtgctggat
     2401 tatgtaaccg tggacagcct ccaggcttcc ctgagtaact tcacccatgt ttccgccgtc
     2461 aaccagaccc gcacctcgct ggattcccta ggcggggcgc tcaccgtaac cattgaggcg
     2521 ggaagcagcc ttatcctgaa cggaacctct aacctgacca cgcttattct gggggaacac
     2581 gcctccctga cgcttcaggg gctcaccgca gatgcggtcg tcgtggacat cacgggaaca
     2641 agcaattaca cactctccct gacagagata cctgccagcc tggacaacat caaattcctg
     2701 aacgacggcg ttctgtacga cgcggccatg tccatggatc ttcaggccaa ttccgccatg
     2761 cttttcgccc aggtgccgga acccggaagc gccgccctgg ccctggccgg actggccccc
     2821 ctcctgtgga ggaggcgcag aaaaatgtcc cattgaggcc cttcgttttc ttcaggccct
     2881 gatggacaaa agaagccccc gccggcgtaa cgccggcggg ggcttcccat gcggaaaaac
     2941 ggcggagaag aaaaggaaaa gcatccatcc ctccccgggc gtttttcatg gaacgggagg
     3001 agatcaggcc cctctgtttt taaacagttc cagtgcgagc tgagaccagg tc
//
LOCUS       DAOMSU010000259         2964 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3398_length_2964_cov_2.786525, whole genome shotgun sequence.
ACCESSION   DAOMSU010000259 DAOMSU010000000
VERSION     DAOMSU010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3398_length_2964_cov_2.786525"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="PK077_09220"
     CDS             complement(<1..231)
                     /locus_tag="PK077_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24517.1"
                     /translation="MMDLAPILTGIAVAGLICQATAVPVPFKVEAILPQAEGAPYATM
                     AAQIGKDMLASLIPYRVLKNGGVTYHLGDKSTP"
     gene            complement(238..759)
                     /locus_tag="PK077_09225"
     CDS             complement(238..759)
                     /locus_tag="PK077_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24518.1"
                     /translation="MDKQSTDHKEETLVAMLASFRKEACYHDTFEEDFLRNFHLRKET
                     DQAAHSAWKLLLERLDNYLQNFRGWQWVYASMSIVTLAAVGVIIASGNMDEPSESYVS
                     STKDKEEILLTEKAVPVSKETIERTSTEVAPTEKPEFTTGDSSYETSAKDNQLKPDSH
                     ADKKPVSRVLIEM"
     gene            complement(867..1439)
                     /locus_tag="PK077_09230"
     CDS             complement(867..1439)
                     /locus_tag="PK077_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRN24519.1"
                     /translation="MEDTELVARAREGDSRAFDELVIRHSRKLHATLYQMTDNYDDAY
                     DIAQEAFSKAYRALRYFNGQSAFYTWLHSIAVNHARNFLKKRNRRMTYSLDDDEYGDH
                     TEKDMNMADETDGADPERRTHLNELQLKINEALKKLSTKHREVVVLHDVKGLNHTEIS
                     ALLGISEGTLRSRLHYAHKELQGLLAEYLS"
     gene            complement(1500..>2964)
                     /locus_tag="PK077_09235"
     CDS             complement(1500..>2964)
                     /locus_tag="PK077_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HRN24520.1"
                     /translation="GNFNMTNTPELNQRMIERGQHPVFGTDTQTVLEEIGYHLDEAHT
                     DLYRALRDSGMPGPEIPHAISSRLNIQEIIHNSAKQWDGGYAIMGAIGNGDYFCLRDP
                     HGIRPCHYLITDEFIAVASERVPLMTVFEVESEQVQDLPPANMLSIKADGTHAITEFT
                     TPLKPAPCSFEKIYFSRGNDPIVYRERKALGAALTPQIVDSLEDRFDKSAITYIPNTA
                     ETAYYGLLEGLRVYRRKRVHAQLLEALRNGTLDENMLDSAILKRWPRGEKIAHKDIKM
                     RTFITQEKSRAQLVSHVYDLTYGAVGPEDVLVAIDDSIVRGTTLRRSILRILGRTNPR
                     KIVIASTAPQIRYPDCYGIDMSELGNFIAFQAAVSLIKQHGQARLLEEVYKACREELA
                     KPKEERRNCVKAIYEGLTEAEISREITRLVTPHDAPCPVEVIFQTIENLHESIEGPCG
                     DWYFTGDYPTPGGYTTVNVAYMRWFEGKGGRAYDLPL"
BASE COUNT          606 a          717 c          861 g          780 t
ORIGIN      
        1 cggggtactt ttgtccccga gatggtaagt cacgcctccg ttttttaaaa cccggtaggg
       61 aatcaaggag gccagcatgt ctttaccgat ttgcgccgcc atggtcgcat acggcgctcc
      121 ttccgcctgg gggaggatgg cttctacctt aaaagggaca ggcacggccg ttgcctggca
      181 aatcaggcct gcaacggcta tgcccgtcaa tatcggagcc aggtccatca tggattgtta
      241 catctcaatg agaacgcggg ataccggttt tttatcggca tgggaatcag gtttcagctg
      301 gttgtctttg gccgatgttt catatgaaga gtctcccgtg gtgaattcag gtttttccgt
      361 gggagcaact tcggttgagg ttcgttcaat agtttcctta cttacgggaa cggctttttc
      421 cgtcaggagt atttcttcct tatccttggt ggaggatacg taagattcag aaggttcatc
      481 catgtttccg gatgcgataa taacgcctac cgcagctaat gtcacgatgg acatggaagc
      541 atagacccac tgccaaccac gaaagttctg cagataattg tccaggcgtt cgagcagcaa
      601 tttccaagca gagtgggcag cctgatcggt ttcctttctg agatgaaaat tgcggaggaa
      661 atcttcctcg aaggtgtcat ggtaacaggc ttccttgcgg aaagatgcca gcatggccac
      721 cagagtttcc tctttatgat ctgtggattg tttatccatc gtcgttgatg ttaagtataa
      781 tagaagttag aatgtgtcag tcccgaattt ggaactatgc gtttgacatg gcggcgggaa
      841 ttgacgttca atattttttt ccctgattaa cttagatatt ccgccagcag cccctggagt
      901 tccttgtggg catagtgcag acgtgatctg agcgttcctt cagagatacc gagaagggcg
      961 gatatttccg tatggttgag cccttttacg tcatgaagaa caacgacttc cctgtgttta
     1021 gtggaaagtt tcttcaatgc ttcgttgatt ttgagctgca attcattcaa atgggtacgg
     1081 cgttccgggt ccgccccgtc cgtttcatcc gccatgttca tgtccttttc cgtatggtcg
     1141 ccgtactcgt cgtcgtccag actgtaggtc attctgcggt tgcgtttttt caggaagttt
     1201 ctggcgtggt tgacggcaat ggagtgcagc catgtgtaaa aggcgctttg tccattaaag
     1261 tagcgcaagg cccggtaggc tttggagaac gcttcctggg cgatgtcata ggcatcgtcg
     1321 tagttgtccg tcatctggta cagggtagca tggagtttgc ggctgtggcg aatgacaagt
     1381 tcgtcaaaag cccgcgaatc tccttcccgt gccctggcga ccagttcggt gtcttccaag
     1441 gtcgtgtatg aaatgggtgc cgacatgttg tcggcagaat acccaaaaaa agggagccgc
     1501 tacaagggca aatcgtatgc acggccccct ttaccctcaa accagcgcat gtatgccaca
     1561 ttaactgtgg tgtatccgcc cggagttggg taatctccgg tgaaatacca gtcgccgcag
     1621 gggccttcta tggattcatg gaggttttcg atggtctgga aaatgacttc cacggggcac
     1681 ggagcgtcat gcggagtgac caggcgggtg atttcccggg agatttcagc ctcagtcaac
     1741 ccttcgtaaa tagccttcac gcaattgcgc cgttcttcct tgggcttggc cagttcttcc
     1801 ctgcatgcct tgtatacttc ttccagcaga cgggcctgtc cgtgctgctt gatcagggag
     1861 acggccgcct ggaaggcaat gaagttgccc agctcggaca tgtcaatgcc gtagcagtcc
     1921 gggtaccgga tttgcggggc agtagatgca atgactattt tccggggatt ggtgcggccc
     1981 aggatgcgga ggatggacct tctcagcgta gtgccgcgga caatggagtc gtcaatggcg
     2041 acgagtacat cttccggccc tacggcgccg taagtgaggt catatacgtg ggaaaccagc
     2101 tgcgcacggc ttttttcctg cgtgatgaac gtgcgcattt tgatatcctt gtgggcgatt
     2161 ttttcaccgc gcggccagcg tttcaggata gcgctgtcca gcatgttttc atccagggtg
     2221 ccgtttctca gggcctccag gagctgggca tgcacgcgct tgcggcggta gacgcgcaag
     2281 ccttccagca ggccgtagta ggcggtttcc gccgtgttgg gaatgtaggt gatagcggat
     2341 ttgtcaaaac ggtcttcaag agaatccacg atttgggggg tcaacgccgc gccgagcgct
     2401 ttgcgttccc ggtaaacgat aggatcgttc ccgcgggaga agtaaatttt ttcaaaggag
     2461 cagggagccg gtttcagagg ggtggtaaat tccgtaatgg catgtgtccc gtctgctttg
     2521 atggagagca tattggccgg cggaaggtct tgaacctgtt cgctttccac ttcaaagacg
     2581 gtcatcaggg gaacgcgttc ggaagccacg gcaatgaatt catccgtaat caggtagtgg
     2641 catgggcgga tgccgtgcgg atcccgcagg cagaagtagt ccccgttgcc gatggctccc
     2701 atgatggcat atccgccgtc ccattgcttg gcggagttat ggatgatttc ctgaatgtta
     2761 agccgggagg aaatggcatg gggaatttcc gggccgggca tgccgctgtc ccgcaaggcg
     2821 cggtagaggt ccgtatgggc ttcatccagg tgatacccta tttcttcaag gacagtctgc
     2881 gtgtccgttc cgaagacggg gtgctggccg cgttcaatca ttcgctggtt caattccggc
     2941 gtattggtca tgttgaagtt gccc
//
LOCUS       DAOMSU010000260         2960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3404_length_2960_cov_2.512565, whole genome shotgun sequence.
ACCESSION   DAOMSU010000260 DAOMSU010000000
VERSION     DAOMSU010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3404_length_2960_cov_2.512565"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PK077_09240"
     CDS             <1..309
                     /locus_tag="PK077_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24521.1"
                     /translation="EYLRSRGVEVLSPDCGWPRVHVEADYSSSAGLGDWLSIELSLGT
                     VGTSSLEWKFAVFHSERPVMKGRYVVVRVGKGGKPQAISESERECLISGFSKGGDACK
                     "
     gene            315..1859
                     /gene="gpmI"
                     /locus_tag="PK077_09245"
     CDS             315..1859
                     /gene="gpmI"
                     /locus_tag="PK077_09245"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HRN24522.1"
                     /translation="MSSKKPVVLVIRDGWGRNPLGPDVAKEYGDATVLADTPFTDYLL
                     ANYPHSLLGASGEDVGLPDGQMGNSEVGHLNLGAGRVVFQDLCRVDNAIKDGSMGENA
                     VLKTAFSQAASSRLHLLGLVSDGGVHSHINHLIGIVKYAYEAGVRDICIHAITDGRDC
                     SPTSGAGFVRQLEEAVRPYGAKIATVVGRFYAMDRDKRWDRNKLAWDAIVLGRGEQCS
                     CSPAEYVEQCYAKGETDEFLKPGIFAYGNEQRVRDNDVVFFFNFRADRARQMSDAFLY
                     PEFDGFDREVTPKVHYVTLTEYDAKYPSPIVFEQEQLNNIFGQIVSEAGKTQLRIAET
                     EKYAHVTFFFNGGVETQFPGEDRILVPSPREVATYDLKPQMSAAEVADKFVEAVDKYD
                     VVIMNFANGDMVGHTGFVEAGIAACEAVDSALEKCVKKVLELGGKLLITADHGNAEHM
                     RNEDGSPNTAHTTNLVDLIYVAADKDQVTLSNGILADVAPTLLSLMGLKQPAEMSGHS
                     LVTPGK"
     gene            2429..2812
                     /gene="rpsL"
                     /locus_tag="PK077_09250"
     CDS             2429..2812
                     /gene="rpsL"
                     /locus_tag="PK077_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRN24523.1"
                     /translation="MPTINQLVRKGRITPEEKSKSRALHSCPQRRGVCLQVMTRTPKK
                     PNSALRKVAKVRLTNGEEVIAYIGGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGAL
                     DCLGVDKRRQGRSKYGAKRPKAAAK"
     gene            2839..>2960
                     /locus_tag="PK077_09255"
     CDS             2839..>2960
                     /locus_tag="PK077_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRN24524.1"
                     /translation="MARRKRVYRKIERRDPRYDSALVGKLISKVMLDGKRSLAER"
BASE COUNT          684 a          730 c          851 g          695 t
ORIGIN      
        1 gagtacctgc gttcccgggg ggtggaggtt ctttcccctg actgcggctg gccccgcgtg
       61 catgtggagg cggattactc cagctctgcg gggctgggag attggctgag catagaatta
      121 agcctgggaa cggtgggaac cagttctctg gaatggaaat ttgctgtatt tcattccgag
      181 cgtccggtga tgaagggaag atatgtggtc gttcgcgtgg gaaagggagg caaacctcag
      241 gcaatttcgg aatcggagag ggaatgcctg atttcaggct tttcaaaagg aggggatgcc
      301 tgtaaataaa gcccatgtct tctaaaaagc ctgtcgttct tgttattcgc gacggctggg
      361 gccgcaatcc gttggggcct gatgtcgcga aagagtacgg tgacgccact gttctggctg
      421 ataccccttt taccgattac ctccttgcca actatcccca cagcctgctg ggcgcttccg
      481 gggaggatgt gggccttccg gacggtcaga tgggcaactc cgaagtaggg catttgaacc
      541 tgggcgcggg ccgcgttgtt tttcaggacc tctgcagggt ggacaatgcc atcaaggatg
      601 gttccatggg ggaaaacgcc gtgttgaaga ccgctttttc ccaggccgct tcttcccgac
      661 tgcacctgct cggcctggtg agcgacggcg gcgtgcacag ccatatcaat catttgatcg
      721 gcatcgtcaa atatgcttat gaagcgggtg tccgtgatat ctgcattcat gccattacgg
      781 acggccgcga ttgttccccg accagcggtg ccggttttgt tcgccagctg gaggaagccg
      841 tcaggccgta cggcgccaaa attgccacgg tcgtcggccg tttctatgcg atggaccggg
      901 acaagcgctg ggaccgcaac aagctggcat gggatgccat cgtgctggga cgcggcgagc
      961 agtgttcctg ctcccctgcc gaatatgtgg agcagtgtta tgcgaagggt gagacggatg
     1021 agttcctgaa gcccggcatt tttgcctatg gcaacgaaca gcgcgtgcgg gataacgacg
     1081 tggtgttctt cttcaatttc cgtgcggacc gcgcccggca gatgagcgac gctttccttt
     1141 atcccgaatt tgacggcttt gaccgtgaag taactcccaa agtgcactat gtgacgttga
     1201 cggaatacga tgccaagtat ccctctccca ttgtttttga acaggaacag ttgaacaata
     1261 ttttcgggca gattgtgtcg gaagccggga aaacccagct ccgcatagcg gaaacggaaa
     1321 agtacgccca cgttactttt ttctttaacg gaggtgtgga gacccagttc cccggagaag
     1381 accgcattct tgttccttcc ccccgggaag tggccactta tgacctgaag ccccagatga
     1441 gcgcggcgga agtggcggat aagtttgtgg aagctgtgga caagtacgat gtggtcatca
     1501 tgaatttcgc caatggggac atggtgggcc ataccggatt tgtggaagcc ggcatcgccg
     1561 cctgcgaggc ggtggacagc gccttggaaa agtgcgtgaa gaaggtgttg gaacttggcg
     1621 gcaagctgct tattacggcg gatcacggca atgccgaaca catgcgcaat gaggatggtt
     1681 ccccaaatac agcccacacg accaaccttg tggaccttat ttatgtggcg gccgacaagg
     1741 accaggtaac cctttccaat ggcattctgg cggatgtcgc tcccacgctg ctgagcctga
     1801 tggggctgaa acagcctgcc gaaatgtccg gacattccct ggtgacccct gggaaatagc
     1861 gggctgctaa gttcattgat gaaatccgtt tccggatgcg ttccggaaac ggattttttt
     1921 atgtcatgct ttccggacag ggcaaaatag gaaagcaggc aggttaacaa taaatagtag
     1981 accggctgcc gtttttatga aaaaattttt cgtgaattct gatggaaagc ttgttttttg
     2041 tgcctctccg gcgcttcatg catttttttc tgcggtaaca tgtcggacat gaagaaatgt
     2101 ttttttgcct gtgaagattt tttgtttcat tctccatagg tggagaactg ctggacgggg
     2161 aggaaaaagg actccgggag cccgaaatgg agttttgaaa ggagcgggga aaatggaacg
     2221 gataaaatat ttaggaaaag atgaataaaa gacttgtcat gatataaaat cacgttatat
     2281 tggccgtccc caaacgtgcc gaaaggcaca agaaatacgt tttctatcgg gtttgcctcc
     2341 ggaaaaaagg cgcgcttcct gcttataaaa ggaatgttcg ccttgaatat cggatcagac
     2401 cgacgaacca caaccaaagc aggaaaacat gccgaccatt aatcagctcg tccgcaaggg
     2461 acgtattact ccggaagaga agtccaaatc acgcgctctt catagctgcc cgcaacgccg
     2521 cggtgtttgt ctccaggtga tgacccgtac gcccaaaaag ccgaactcgg ctcttcgtaa
     2581 agtggctaaa gtccgtctta ccaatggtga agaagtgatc gcctacattg gcggcgaagg
     2641 tcataacctt caggaacact ccatcgtgct tgttcgcggc ggtcgtgtga aagacttgcc
     2701 gggtgttcgc taccatatcg tccgcggcgc tcttgactgc ctcggtgtcg acaagcgccg
     2761 ccagggccgc tccaagtatg gcgccaagcg tccgaaggct gctgccaagt aaaccaaaca
     2821 tcttacctac tatatattat ggctcgccgt aaacgcgttt atagaaaaat cgaacgtcgt
     2881 gacccccgtt acgacagcgc tcttgttggc aaactgatca gcaaggttat gctggatggc
     2941 aagcgctccc ttgcggaacg
//
LOCUS       DAOMSU010000261         2951 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3421_length_2951_cov_2.231699, whole genome shotgun sequence.
ACCESSION   DAOMSU010000261 DAOMSU010000000
VERSION     DAOMSU010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3421_length_2951_cov_2.231699"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="PK077_09260"
     CDS             complement(<1..419)
                     /locus_tag="PK077_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24525.1"
                     /translation="MIRKTSKRRGTSVIAALVALCAVVGYGLTEWAPLDNEPQAVPAL
                     QREGRQTVREKVEIPDKGEPVRLLTMNAGNYFVPEDPRRSNFQVKYKPVEAREAVAEL
                     VRQSGAEIVGLCEMGGEAAVRDLQMRLKRKGVHLPYKV"
     gene            543..1229
                     /locus_tag="PK077_09265"
     CDS             543..1229
                     /locus_tag="PK077_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRN24526.1"
                     /translation="MNVSPLQPHNESQPDEQTLSQYAEHTRKSLIARLENWEDQRTWD
                     EFYRTYWRLIYSVALKAGLREDEAWDVVQETILSIAKQSRKNIYKPEQGSFKLWLWNM
                     TRWRINDQFRKRKKDTAMLMNPDAPGTLEHPIDKIPDEGKNNFDQVWEREWQNNLLQA
                     ALERVKAKVSPKQFQIFDYYVLREWDAAKVRRQLGVTIAQVYLAKHRVGSALKKELQF
                     LQSQEKEKEK"
     gene            1360..>2951
                     /locus_tag="PK077_09270"
     CDS             1360..>2951
                     /locus_tag="PK077_09270"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRN24527.1"
                     /translation="MPPSRNRHSAQRVFTWDKIKMALAAAEEGFYIWNIKTGVIHYTD
                     RCLTMMGASRKEKAPNIFTQPELTIHEEDQAFFSQEVRRYLDGHSHVPMRIEIRMKKL
                     NSKSWSWVRVNGLARRDKQRRPVMLVGVWVNITRRKTAELRAAEDRDLFHTLIEHIPD
                     SIYFKNRESRFVLANTATANKLGVPTPADLTGRTDDYFFDQTMSDISRKEEMDIMVTG
                     RPIRARLHHETWLHKDDSWSQISKFPWYGRNGELKGIVGISSDVTKLVKTEIKATETA
                     RILEERNRTLEKEIDLAREIQFALLPYEIPSRSHTEHGLTRHADFHHIFTPSEGVAGD
                     WFDAFPVGNSGVGAIVCDVMGHGIRAALIASMLRGLMEQLSHLADNPAAFLTSLNHQL
                     AKILQRANTTMFASAVYIYLDLETGVMTASTAGHPHPIILGPDGVARKMPLPRGIALG
                     LLDDATYHNAQFSLLAGARILMYTDGLTEAANQDGEEMGVERLIDYFNNSSPHSTKDF
                     VHQALTCVAKFTGCTNQADDIC"
BASE COUNT          722 a          872 c          740 g          617 t
ORIGIN      
        1 actttgtacg gcaaatgaac tccttttctt ttcagccgca tttgcaagtc acgaacggca
       61 gcctccccgc ccatttcaca cagccccacg atttccgccc ccgattgacg aaccagctcc
      121 gccacggctt cacgggcttc cacaggtttg tattttacct gaaaattgct tctcctgggg
      181 tcttcaggaa caaagtagtt gccggcattc atggtcagaa gacgaacggg ctcccccttg
      241 tcggggattt ccaccttctc ccgaaccgtc tgccgccctt ccctctggag ggcaggaacc
      301 gcctgcggtt cattatccag cggcgcccac tccgtcaaac cgtaccctac caccgcgcac
      361 aacgcaacca gggccgcgat gacggaggtt cccctgcgtt ttgacgtttt cctgatcatg
      421 tccgtttccc tgaaagattt tctccatggc gaacgtagtg aatgaacaag ccggaccgta
      481 tggaattttc cggcctatta ctcggctttc gcttcacttc catccgtagt tcatgataaa
      541 ccatgaatgt gtccccattg caaccccata acgaatccca gcctgacgaa cagacacttt
      601 cccaatacgc ggaacatacc aggaaaagcc tgatcgcgcg tttggaaaac tgggaagacc
      661 agcgcacctg ggatgaattt taccggacgt attggcggct tatctattcc gtcgccctca
      721 aggccggatt aagggaagac gaggcctggg acgtggtaca ggaaaccatt ctttccatcg
      781 ccaagcagag caggaaaaat atttacaaac cggaacaggg ttccttcaaa ctctggctct
      841 ggaacatgac ccgctggcgc atcaacgacc agttccgcaa aaggaaaaag gacacggcca
      901 tgctgatgaa tccggatgcc ccaggcacgt tggaacaccc catcgacaaa atcccggatg
      961 aagggaagaa taattttgac caggtatggg aacgggaatg gcagaataac cttcttcagg
     1021 cggctctgga acgcgtcaag gcaaaagtgt cgcccaagca gttccaaatt tttgactatt
     1081 atgtcctgcg ggagtgggac gcggccaagg tccgccggca gctcggagtc acgattgccc
     1141 aagtttatct ggcaaagcac cgcgtaggaa gcgctttgaa gaaagagctg caatttcttc
     1201 aatcccagga aaaggaaaaa gaaaaatagg agcccatctt aaaaaccagc cggcgtcctt
     1261 gatacgcatc cctccggcca gacctccttt cagaaaggat tcctttattt tgcctcttgc
     1321 caagacagcg gttttgtcgt tagattaacg gcaactgcaa tgccgccctc acgcaacaga
     1381 cattccgccc agagagtttt tacctgggac aaaatcaaaa tggccctggc ggcggcggaa
     1441 gaaggatttt acatctggaa tattaaaacg ggcgtcattc attacacgga ccgctgcctg
     1501 accatgatgg gggccagccg caaggagaaa gctcccaata tcttcaccca gccggagctt
     1561 accatacacg aggaagacca ggcttttttt tcccaggaag tgcgccgtta tctggacggc
     1621 cactcccatg tgcccatgcg cattgaaatc cggatgaaga agctgaactc caaaagctgg
     1681 agctgggtcc gcgtcaacgg cctcgcccgc agggacaaac agcgccggcc cgttatgctg
     1741 gtgggcgtat gggtcaatat tacccggcgg aaaacggcgg aactgcgcgc tgcggaggac
     1801 agggacctgt ttcataccct gattgaacac attcccgaca gcatctattt caagaaccgg
     1861 gaatcacgtt tcgtcctggc gaataccgcc acagccaata agctgggcgt accgacgccg
     1921 gcagacctga ccggacgcac ggacgattac ttttttgacc aaacgatgtc cgatatttcc
     1981 cgcaaggagg aaatggacat catggttacc ggacgcccca tccgcgcccg ccttcatcat
     2041 gagacatggc tccacaagga tgattcctgg agccagatca gcaagttccc gtggtacgga
     2101 cgcaacggag agctaaaggg aattgtaggt atttccagtg atgtcaccaa actggtgaag
     2161 actgaaatca aggccacgga aacggcccgc atcctggagg aacgcaacag aactctggaa
     2221 aaggaaatag atttggccag ggaaatccag ttcgccctgc ttccctacga aattccctcc
     2281 cgttcccata cggaacacgg cctgacccgc catgcggatt ttcaccatat tttcactcct
     2341 tcggagggtg ttgcaggaga ctggttcgat gcttttcccg taggcaattc cggcgttggc
     2401 gccatcgttt gcgacgtgat gggccacggc atccgcgccg ccctgatcgc ctccatgctc
     2461 cgcggcctga tggaacaatt gtcccacctg gcggataatc cggcggcttt ccttacttcc
     2521 ctcaaccacc agctcgccaa aatcctgcaa cgggcgaaca ccaccatgtt cgcctccgcc
     2581 gtttatattt acctggatct ggaaaccggg gtcatgacag cctccacagc cggacatccg
     2641 catcccatca ttctggggcc ggacggggtc gcccgcaaaa tgccgctgcc cagaggcatc
     2701 gccctgggac tgctggatga cgctacgtac cataatgccc agttttcgct cctggcgggg
     2761 gcccgcatcc tgatgtacac ggacggcctg acggaagccg ccaaccagga cggggaagaa
     2821 atgggcgtgg aaagactgat tgactatttc aacaattctt cccctcacag caccaaggat
     2881 ttcgttcatc aggcccttac ctgcgtagcc aaatttaccg gttgcaccaa tcaggccgac
     2941 gatatctgca t
//
LOCUS       DAOMSU010000262         2940 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3442_length_2940_cov_2.632582, whole genome shotgun sequence.
ACCESSION   DAOMSU010000262 DAOMSU010000000
VERSION     DAOMSU010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3442_length_2940_cov_2.632582"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="PK077_09275"
     CDS             <1..982
                     /locus_tag="PK077_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRN24528.1"
                     /translation="LMNMDNLNRRLPGQVKMLLPHSRLATFNPSSPPPEEPRQAGALP
                     FANAPLIFLPGDAGAVPLQDHKKMLTWTLTLIWSMVLLGAAGVIWLMLGTFRLEQRRG
                     DFVSAVTHELRTPLTSFSLYTEMLEDGMVPEQKKPEYYANMQRECRRLEHLIENVLSY
                     SRLQRNAIRRARDTLTCQELFEPIAEKIERRLREADISFSFALAQPIRILPIHTDAVS
                     VEQIMDNLTSNAIKYAKGENAKVQLTVQADRHNIVIRFRDNGPGISPKNRKLVFKPFR
                     RTKDATNSRKPGVGLGLALARDTARSLGGDLKLEFGTLGGASFLLTIPKS"
     gene            1223..2155
                     /locus_tag="PK077_09280"
     CDS             1223..2155
                     /locus_tag="PK077_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRN24529.1"
                     /translation="MSKRILITGGAGFIGSHLSERLLREGHEVICMDNFFTGSKQNIL
                     HLTDYPGFEVIRHDVTVPYVMEVDQIYNLACPASPPHYQFDPIHTMKTSVLGALNMLG
                     LAKRCKARILQASTSEVYGDPMVHPQPETYWGNVNPVGVRSCYDEGKRCAETLFMDYR
                     RMNGVDVRIIRIFNTYGPRMNPNDGRVVSNFIVQALKGEDITIYGTGKQTRSFQYVDD
                     LVEGMVRMMDTEGFSGPVNLGNPEEFTMLELAEKVIEMTGSSSKTVFRPLPLDDPTQR
                     KPDIRLAEEKLGWKPHITLEKGLEKTIAYFRSIL"
     gene            2341..>2940
                     /locus_tag="PK077_09285"
     CDS             2341..>2940
                     /locus_tag="PK077_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HRN24530.1"
                     /translation="MEHASIREIYSVQATVRTYESGADGLMKPETVLHWFQEIAEAHA
                     SCLGFGYDFVTSRSLAWVEVRLDASVNRLPRWKETVELRTWTAQETPLLARRNLEIRD
                     AQGNCIVAASCLWAVIDIRRRRPVPLNRHISFFPDTPCKETVAPVSMDISGLLPSIRE
                     WTAERRDTDFNRHINNAAYLVWALDSLPDSWLKNHQLTGI"
BASE COUNT          698 a          895 c          766 g          581 t
ORIGIN      
        1 cctgatgaac atggacaatc tgaacagacg cctgcccggc caggtaaaga tgctgcttcc
       61 ccacagccgc ctggcaacct ttaacccgtc ctccccgccc ccggaggaac cccggcaggc
      121 gggcgcactc ccctttgcca acgcccctct gattttcctt ccgggagacg ccggagccgt
      181 tcctctccag gaccacaaaa aaatgctgac ctggacgctc accctcatct ggagcatggt
      241 gctcctgggt gcggctgggg tcatctggct catgctggga accttccgcc tggaacagcg
      301 ccgcggggac ttcgtctccg ccgtcaccca tgaactccgg acgcccctca cgtccttctc
      361 cctgtacacg gaaatgctgg aagacggcat ggtgccggaa cagaaaaagc cggaatacta
      421 tgccaacatg cagcgggaat gccgccgtct ggaacacctg attgaaaacg tactctccta
      481 ctcccggctt cagcggaacg ctattcgccg cgcgcgggac acgctcacct gtcaggagct
      541 ttttgaaccc attgcggaaa aaatagaacg ccgcctccgg gaggcggaca tcagcttctc
      601 ctttgccctg gcccagccca tccgcatcct tcctattcat acggatgccg tttccgtaga
      661 gcagattatg gacaacctga cttccaacgc cattaaatac gccaaggggg aaaacgccaa
      721 agtccagctc accgtccagg cggaccgcca caacatcgtc atccgcttcc gcgacaacgg
      781 cccaggaatt tccccgaaaa accgcaagct tgtcttcaag cccttccggc gcacaaaaga
      841 cgctaccaac agccggaaac ccggcgtagg cctggggctg gcgctggcaa gggatacggc
      901 acgttccctg gggggagacc tgaaactgga attcggcacc ctggggggag ccagctttct
      961 gctgacgatt cccaaatcct gaacctgccg gcgggacagc cgggaaccaa ccggaattcg
     1021 cctcccggtc cgtacggaaa aaccttcctg gttcaccccc tgcctttccc ggacattcct
     1081 gtaatattcc gtccgggatg ctatgcgctt gccttcccgt gacggatatg tgacattgaa
     1141 ccgcaacgca cggactgggg aaaactttcc gcttgcttcc cttccggcgc ggcggaacga
     1201 acaaacgcat ggacatgcca tcatgagcaa aaggattttg attaccggag gcgccggctt
     1261 catcggctcc catctcagcg aacggcttct cagggaaggt catgaagtta tctgcatgga
     1321 caacttcttc acgggcagca aacaaaacat cctccacctg acggattatc ccggatttga
     1381 agtaatacgc catgacgtta ccgttcctta tgtcatggaa gttgaccaaa tctacaacct
     1441 ggcctgcccc gcctctcctc cccattatca gtttgacccc atccacacaa tgaaaacctc
     1501 cgtgctggga gctctgaata tgctgggcct tgccaaacgg tgcaaggcca gaatcctcca
     1561 ggcctccacc agcgaagtat acggtgatcc catggtccat ccccagccgg aaacctactg
     1621 gggaaacgtc aaccccgtgg gcgtccgctc ctgctatgac gaaggcaaaa gatgcgcgga
     1681 gaccctgttc atggactacc gccgcatgaa cggggtggac gttcgcatca tccgcatctt
     1741 caacacttac ggaccgcgaa tgaaccctaa cgacggaaga gtagtctcca acttcatcgt
     1801 ccaggcgctg aagggggaag acatcaccat ttacggaacg ggcaaacaga cccggagttt
     1861 ccagtacgtg gacgacctgg tggaggggat ggtgcgcatg atggatacgg aaggtttttc
     1921 cggcccggtc aacctgggta atccagagga attcaccatg ctggaactgg cggaaaaagt
     1981 cattgaaatg acaggatctt cttccaaaac agtcttccgt cctctgcctc tggacgaccc
     2041 gacccagcgc aaaccggata ttcggcttgc agaagaaaaa ctgggctgga aacctcatat
     2101 tacgctggaa aaaggattgg aaaaaaccat tgcctatttc cggagcattc tttagaaatc
     2161 agggaatgcc gtccacctca gcggatgctg aaaggaattc cattgtatgc cgcgccccag
     2221 gcaggcagcc cggaaaatac ggaggccatt catataccgg gagaggcggc atgccaaacg
     2281 gttgaatatt acccgttttt aactggctct gaaagaatgg ttttactagc ataagatgtt
     2341 atggaacatg ccagcatcag ggaaatctat tccgttcagg caaccgtccg cacttacgaa
     2401 agcggcgcgg atggactgat gaaaccggaa acggttctgc actggtttca ggaaatagcg
     2461 gaagcccatg cctcctgcct ggggttcggc tatgattttg taacgtcccg cagcctggcc
     2521 tgggtggaag tgcgcctgga tgcgtccgtc aatcgccttc cccggtggaa ggaaacggta
     2581 gaactccgga cctggacggc ccaggaaacg cctctgctgg cgcgccgcaa cctggaaatc
     2641 agggatgccc aaggcaactg cattgttgca gcaagctgcc tgtgggctgt cattgatata
     2701 cgccgcaggc ggccggtccc cttaaacagg cacatcagct ttttcccgga taccccatgc
     2761 aaagaaacgg tagctccggt atctatggac atttcaggac ttctgccgag tatcagggaa
     2821 tggacggcgg aacgccgtga tacggatttc aaccgtcaca tcaacaatgc cgcctacctg
     2881 gtctgggctc tggactccct tccggactcc tggctaaaga accaccaact gacgggaatt
//
LOCUS       DAOMSU010000263         2925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3465_length_2925_cov_2.963763, whole genome shotgun sequence.
ACCESSION   DAOMSU010000263 DAOMSU010000000
VERSION     DAOMSU010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3465_length_2925_cov_2.963763"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..722
                     /locus_tag="PK077_09290"
     CDS             156..722
                     /locus_tag="PK077_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24531.1"
                     /translation="MIKSLLAGMMVAAVCAVQAGEYGDSAVRAARELSGAVKTGDMMW
                     MIEKMYPPMKKQLVASFPGGEASFMETMREKMQAAAAVMKERGMVVETYEIGQPAAEH
                     LVKGGGEVLVVLPTRMVISMKRPDGVPVKVENTGVLVLVKDLKDKGPWTFIDASRMNA
                     NGLRSMFYDLPEKGNLPPVSSRQLPVGQ"
     gene            742..2727
                     /locus_tag="PK077_09295"
     CDS             742..2727
                     /locus_tag="PK077_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRN24532.1"
                     /translation="MKAAGEEGMEGVSAFEAYVHRAFAPDGLFSRARDFEYREEQQSM
                     ALAVARALQVDAPLLVEAGTGVGKSLGYLLPAVKFALDFDRKAVISTHTINLQEQLFN
                     KDIPLLRTALGIDFSAALLKGRQNYLCHTRLRRALAQMDSLFTQGEAAELKRIQDWAL
                     RTQDGTLSDMAFRPSSKVWAMVCSEPHACSMRHCGPSCPYQAARKRVLEAKVVVLNHT
                     LFFGLMAQAEDSEEAGFVFPGDFVILDEAHMIENIAARQLGVQLSEPHLNYELLRMYN
                     PRTHKGLLKPLNNPSLFQRVQDVMDASGLFFQNARDDLGFAGSGKIVRILQPEWSQDI
                     LSQPLMELIGELKTEREKQEENAAVKDELADMAARMEEAQASLKVLMDMTEEGHVYWA
                     ERSGPDGRNMSVCSAPVEVGDILRERLFSAGRSAILTSATLGTGDADMSYFAGRVGAE
                     SVRKLQIGSPFNYREQMRLIVARSMPEPDQPEYAEVLPEWIKRYLAESRGRAFVLFTS
                     YRLMVQAAEKVRPFCEEQGWTLYVQGQDMQRHAMLEAFRKDVDSVLFGTDSFWTGVDV
                     PGESLSNVIVTRLPFEVPDHPLVESRFESIRERGGNPFYEYSVPVAILKLRQGVGRLI
                     RTRSDSGMVVILDPRVATKRYGMRFLKSLPEARLEFV"
     gene            complement(2735..>2925)
                     /locus_tag="PK077_09300"
     CDS             complement(2735..>2925)
                     /locus_tag="PK077_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cobalt ABC transporter ATP-binding protein"
                     /protein_id="HRN24533.1"
                     /translation="DLDMVMDLCTRCIVMKDGSILADAPVPDIFADRSLLEEARLEQP
                     LSYLLMRQERQRNNSGAS"
BASE COUNT          555 a          719 c         1014 g          637 t
ORIGIN      
        1 ccggacgcgc gctgatggag gatgccgccc gggagctgga ccccacttta tggatgtggt
       61 ccggcctggt gatgcaggat gacgcgcggt aaaaacatga ttccgcagga ggccttgcct
      121 tgacggaggg ggatgcatgc cgttacactc cgtccatgat taagagtttg ttggcaggca
      181 tgatggtggc ggctgtatgc gccgtccagg ccggtgaata tggggacagc gccgtccggg
      241 ccgcccggga gctttccggg gcggtgaaga cgggcgacat gatgtggatg attgagaaga
      301 tgtacccgcc catgaagaag cagcttgttg cttcctttcc aggcggggag gcctccttca
      361 tggagaccat gagggaaaag atgcaggccg ccgccgccgt gatgaaggaa cgcggcatgg
      421 tggtggagac gtatgaaata ggccagcctg cggcggaaca tctggtgaag ggaggcggag
      481 aggtgctggt ggtgcttccc acgcggatgg tcatttccat gaagcgtccg gacggggtgc
      541 ccgtgaaggt ggagaatacc ggtgttctgg tgctggtgaa ggatttgaag gataaggggc
      601 cctggacgtt catcgacgct tccagaatga atgcgaacgg cttgcgctcc atgttctatg
      661 acctgcctga aaaggggaat cttccgccgg tttcttcccg ccagcttccc gtgggccagt
      721 aaggggggca ggttcccaca tatgaaggcc gccggggaag agggcatgga aggcgtcagc
      781 gcttttgagg cttatgtcca ccgggcattt gccccggacg gattgttttc ccgcgccagg
      841 gattttgagt accgggagga gcagcagagc atggccctgg ctgtggcgag ggcgctccag
      901 gtggatgcgc cgctgctggt ggaggccggc acaggcgtgg gcaaatccct ggggtatttg
      961 ctgccggcgg tgaagtttgc tttggatttt gaccggaagg cggtgatttc cacgcatacc
     1021 atcaatttgc aggagcagtt gttcaacaag gatattccgc tgctgcgcac cgccctgggg
     1081 attgattttt ccgccgccct gctgaagggc aggcagaatt acctgtgcca tacccgcctg
     1141 cggcgtgcgc tggcgcagat ggattccctc tttacgcagg gtgaggccgc ggagttgaag
     1201 aggatccagg actgggccct gagaacgcag gacggcaccc tgagcgatat ggcatttcgg
     1261 ccgtcttcca aggtatgggc catggtctgc agcgaaccgc atgcgtgcag catgcgccat
     1321 tgcggccctt cctgtccgta ccaggcagcc cgcaagcggg ttctggaggc caaagtagtg
     1381 gtgcttaacc ataccctgtt tttcgggctg atggcccagg cggaggattc cgaggaggcg
     1441 gggttcgtgt ttcccgggga ttttgtgatt ctggacgagg ctcacatgat tgagaacatc
     1501 gccgcccgcc agctaggcgt ccagctctcg gaaccgcatt tgaattatga attgctgcgg
     1561 atgtacaatc cgcgcacgca caaggggttg ctgaaaccgc tgaacaatcc gtccctgttt
     1621 cagcgggttc aggacgtgat ggacgcctcc ggcctgtttt tccagaatgc gcgggatgac
     1681 ctggggtttg ctggttccgg aaagattgtc cgcattcttc agccggagtg gtcgcaggat
     1741 attctttctc agcctttgat ggaactgatc ggggaattga aaacggagag ggagaaacag
     1801 gaggaaaatg cagccgtgaa ggatgaactg gcggatatgg ccgcccggat ggaggaggcg
     1861 caggcttccc tgaaggtgct gatggacatg acggaggaag gtcacgtata ttgggcggag
     1921 cgttccggtc cggatggaag gaacatgagc gtttgctccg ctcccgtgga ggtgggagat
     1981 attttgaggg aacgcctgtt ttccgccgga cgttccgcca ttctgacttc cgccacgctg
     2041 gggaccggag atgcggatat gagttatttt gcggggcgcg taggggcgga aagcgttcgg
     2101 aagcttcaga ttggaagccc gttcaattac cgggagcaga tgcggctgat tgtagcccgc
     2161 tccatgccgg agccggacca gccggagtat gcggaagttt tgccggaatg gattaaaagg
     2221 tatctggcgg agtcccgcgg cagggctttc gtgctgttca ccagctatcg gctgatggtc
     2281 caggcggcgg agaaagtgcg ccctttttgc gaggaacagg gctggacgct gtatgtgcag
     2341 gggcaggata tgcagcggca tgccatgctg gaggcgttcc ggaaggatgt ggacagcgtg
     2401 cttttcggaa cggacagttt ctggaccggg gtggatgtgc cgggagaatc tctttccaac
     2461 gttattgtca cgcgcctgcc gtttgaagtg ccggatcatc cgcttgtgga gtcccgcttt
     2521 gagagtatca gggaacgcgg ggggaatccg ttttacgaat attccgtgcc tgtagccatt
     2581 ttgaagctgc ggcagggggt agggcggctg atccgcacca ggagcgattc cggcatggtg
     2641 gtgattcttg atccgcgcgt agctaccaag aggtatggga tgcgttttct caagtccctg
     2701 ccggaggccc ggctggagtt tgtatgaggt tttttcagga tgccccggaa ttgttccgtt
     2761 gcctctcctg ccgcatcagg aggtagctga ggggttgttc caggcgcgct tcttccagca
     2821 gggagcggtc cgcgaagatg tccggaacgg gagcgtctgc caggatggag ccgtctttca
     2881 ttacgatgca gcgcgtgcac agatccatga ccatgtccag atcat
//
LOCUS       DAOMSU010000264         2919 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3474_length_2919_cov_2.232193, whole genome shotgun sequence.
ACCESSION   DAOMSU010000264 DAOMSU010000000
VERSION     DAOMSU010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3474_length_2919_cov_2.232193"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PK077_09305"
     CDS             complement(<1..277)
                     /locus_tag="PK077_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HRN24534.1"
                     /translation="MVYRICKSIELESGHLLSKHPGNCKFPHGHTRSVEMVFRAETLD
                     ANDMVLDFKAIKQMMGDFLQQFDHGMGQIGIVFDQKHGARHLLIAAPG"
     gene            453..989
                     /locus_tag="PK077_09310"
     CDS             453..989
                     /locus_tag="PK077_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24535.1"
                     /translation="MAYLSWSFLKMGEKAERSTSFLFLLMAGGPVLFAAFFAFVAGMT
                     AILYAGPVLALMLLPFYLPCALREEKTRNACSRNILFGRKPLAVSFRVIARDWVYVIY
                     AVLPALPFLFPGGNMAQAGLGNEQSLTAFFLMWISLYAVFAVLAVFLGKARYLRPGRA
                     LLAAGVNLALMLVYYLFA"
     gene            complement(1131..2738)
                     /locus_tag="PK077_09315"
     CDS             complement(1131..2738)
                     /locus_tag="PK077_09315"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HRN24536.1"
                     /translation="MEKDDTQYRLITSDYVSVGDFEGHPMLKVEPEGLRLLAAEAFHD
                     VAFFLRPAHLKQVAAILDDPEASDNDKSVALTMLRNAEVASAGILPFCQDTGTATIVG
                     KKGQQVWTGCDDCEQLSHGVYDAYTQNNLRYSQTVALDMYTEKNTGTNLPAQIDLEAV
                     EGMKYSFLFVAKGGGSANKTYLYQETKALLNPATLKKFLVEKMSTLGTAACPPYHIAF
                     VIGGTSAEKCLKTVKMASTKYYDNLPTTGNEHGRAFRDLELEKELHEEACKLGLGAQF
                     GGKWYALDVRVIRLPRHGASCPVGLGVSCSADRNAKAKITPEGIFIEQLETNPGQYIP
                     EALRMTSSEAVSIDLNRPMKEVLKELGKYPVTTRLSLNGTIIVARDIAHAKLKERLEK
                     GEGLPQYMKDHPVYYAGPAKTPEGYPSGSFGPTTAGRMDSYVDLFQENGGSMIMIAKG
                     NRSQQVTDACRKHGGFYLGSIGGPAAILAKNNIKKVELLEYPELGMEAIWKIEVEDFP
                     AFILVDDKGNDFFAKIREGWACAGCGH"
BASE COUNT          528 a          794 c          878 g          719 t
ORIGIN      
        1 gcccgggcgc ggcgatgagc agatgtcgcg caccgtgctt ctggtcgaaa acgatgccga
       61 tctggcccat gccatggtcg aactgctgga gaaaatcccc catcatctgc ttgatagcct
      121 tgaaatccaa caccatgtca ttggcatcca gcgtttccgc tcggaacacc atttccacgg
      181 agcgcgtatg gccatgcggg aatttgcaat taccgggatg tttggacaga agatgcccgc
      241 tttccagttc gatggatttg caaattcggt agaccatgga ggcaggataa cgtgggcgcg
      301 ggcgcggggc aatcccaatc agagtctgtc tggttctcat tttgtctcgt acgtagggat
      361 ggggctatgc tgctccggat gtttttgggc ggctttccct tgcgcatggc ctcgatttct
      421 ggcagacttc aaaaaaacca tatttttacc ctatggctta tctttcatgg tctttcctga
      481 aaatggggga gaaggcggag cgtagcacat cctttctttt cctgctcatg gcgggcgggc
      541 ctgtcctctt tgccgctttc tttgctttcg tagcgggaat gaccgccatt ttgtatgccg
      601 ggcccgtcct ggcgctgatg cttcttccct tctatttgcc ctgcgctctg agggaggaaa
      661 agacgcggaa tgcctgctcc cgtaatattc tgtttggcag gaagccgctg gcggtctctt
      721 tcagggtgat agctagggat tgggtatatg tcatttacgc cgtgcttccg gcgttgccgt
      781 ttcttttccc tggcgggaac atggcccagg ccggcctggg gaatgagcag agcctgacag
      841 catttttcct gatgtggatc tccctgtacg ccgtctttgc ggtgctggcg gtttttcttg
      901 gaaaggcgcg gtatttgagg ccgggcaggg cgctgctggc tgccggcgtc aatctggcgc
      961 tgatgctggt ttattacctg tttgcgtgaa gaagcgcatg atgaataccg gaagtttccc
     1021 ccccggatat tcacgccgtc cggaccggga ggaaggagcg agaccggagc attaaaaagc
     1081 cggagccccg aaggactccg gccgcgaatc attttgtttc cgggtcaggc ttaatgcccg
     1141 caaccggcgc aggcccatcc ttcgcggatt ttcgcgaaga aatcgttgcc cttgtcatct
     1201 accagaatga aggcggggaa atcttccact tcaattttcc agatggcttc catgcccagt
     1261 tccgggtatt ccagcagttc cacttttttg atgttgtttt tggccagaat ggcggccggg
     1321 ccgccgatgg agcccaggta aaagccgccg tgcttccggc aggcatccgt tacctgctgg
     1381 ctgcggttgc ccttggcaat catgatcatg gagcctccgt tttcctggaa caggtctacg
     1441 taggaatcca tgcggcctgc ggtcgtgggg ccgaaggaac cggaggggta gccttcagga
     1501 gttttggccg ggccggcgta gtacacgggg tgatccttca tatactgagg cagcccttcg
     1561 cccttttcca ggcgttcctt cagcttggcg tgggcgatgt cgcgcgccac aatgatggtg
     1621 ccgttcaggc tcaggcgcgt ggtgacgggg tacttgccca gttctttcag aacttccttc
     1681 atcgggcggt tcaggtcaat ggagacagct tcgctggagg tcatgcgcag ggcttccgga
     1741 atatactggc cggggttggt ttccagctgt tcaataaaga tgccctccgg cgtgatcttg
     1801 gctttcgcgt tgcggtccgc agagcaggag actcccaggc caaccggaca ggaggcgccg
     1861 tggcggggca gccggataac gcgcacgtcc agagcgtacc acttgccgcc gaactgggcg
     1921 cccaggccca gtttgcacgc ttcttcatgc agttcctttt ccagttccag gtcacggaag
     1981 gcgcggccgt gttcgttccc cgtagtcggc agattgtcgt agtacttcgt ggaagccatc
     2041 ttcacggtct tcaggcattt ttccgcagag gttccgccaa tgacgaaagc gatgtgatag
     2101 ggagggcagg ccgccgtgcc cagagtactc atcttttcca ctaggaactt tttcagcgtg
     2161 gccgggttga gcagggcctt cgtttcctgg taaaggtaag ttttgttggc ggagccgccg
     2221 cccttggcga cgaacaggaa ggagtatttc atgccctcca ccgcttccag gtcaatttgg
     2281 gcgggaaggt tggtgccggt gtttttctcc gtgtacatat ccagcgccac cgtctgggaa
     2341 tagcgcaggt tgttctgggt ataggcgtcg taaacgccgt gggaaagctg ttcgcagtcg
     2401 tcacagccgg tccatacctg ctggcccttc ttgcctacga tggtggcggt gcccgtgtcc
     2461 tggcagaagg gcaggatgcc ggcggacgcc acttccgcgt tgcggagcat cgtcagggcc
     2521 acgcttttat cattgtcgct ggcttccgga tcgtccagaa tggcggccac ctgcttcagg
     2581 tgggcggggc gcaggaaaaa ggccacgtca tggaaggctt ccgctgccag caggcgcagt
     2641 ccctccggtt ccactttcag catgggatgt ccttcaaagt cgcctacgct cacatagtct
     2701 gacgtgatga gacggtattg ggtgtcgtcc ttttccatgg gaaaaggatc ctgataatga
     2761 aaatccggtg ttgccataac gggagccaat atagcgaagc tccgaatccg gttcgagcaa
     2821 aaaaatcagg gatgccccca tgcggagccc atgcccgaaa aggttccgga tacttgactt
     2881 tgacgcccgc ctgcattact ttttccccgc ttaagtgta
//
LOCUS       DAOMSU010000265         2904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3509_length_2904_cov_2.400140, whole genome shotgun sequence.
ACCESSION   DAOMSU010000265 DAOMSU010000000
VERSION     DAOMSU010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3509_length_2904_cov_2.400140"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="PK077_09320"
     CDS             <1..211
                     /locus_tag="PK077_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRN24537.1"
                     /translation="IDTCCRLRANRRGLSIRDAEDGACMMLTKDGCRINPVKPRQCRD
                     FPNKWNFPGWRDLCRAREVGEEREA"
     gene            complement(307..1614)
                     /gene="aroA"
                     /locus_tag="PK077_09325"
     CDS             complement(307..1614)
                     /gene="aroA"
                     /locus_tag="PK077_09325"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HRN24538.1"
                     /translation="MNLHSHSISSLQGVLTVPGDKSISHRAAILGGLAQGVTEVDNFL
                     CSEDCLNTLRAMEQLGAKVDVLEERQGYGPVRFRITGVAMSPKAPERPIDCGNSGTGM
                     RLLAGMLAACPFDSEMFGDASLSSRPMGRIMQPLEQMGARIEARGTKPGCAPLSIHGG
                     RVHPISYALPMASAQVKSAILLAGMFADGTTTVRQPAATRDHTERLFRHFGVPCTVDG
                     LTVGTCGPALPVAHNLTVPADISSAAFWMVAAASRPGSRLTLRQVGLNKTRNAVISAL
                     QRMGARMDIVPTSPEDAGEPYGDITVYGSDSLHGTSLLPEEIPNLIDEIPILAVAGAL
                     GRGDFIVRNARELRVKETDRIATTAANLRLMGVDVEEFDDGMVVHGGTPLKGTELSSY
                     GDHRIAMSFLVAGLSAQGETVVTDAECINTSYPGFERDLAQFL"
     gene            1982..2400
                     /gene="rnpB"
                     /locus_tag="PK077_09330"
     ncRNA           1982..2400
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK077_09330"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2541..>2904
                     /locus_tag="PK077_09335"
     CDS             2541..>2904
                     /locus_tag="PK077_09335"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420923.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRN24539.1"
                     /translation="MEKLVVHGGFTLRGAVNISGSKNASLPILAASLLTDEPVVVRRV
                     PDVSDTNFMVQIMGQLGASVERSSGNVRVEARSLHSEAAYEQVRKMRASICLMGPLMA
                     RMQRCVIPLPGGCVIGDRP"
BASE COUNT          601 a          790 c          905 g          608 t
ORIGIN      
        1 tattgacaca tgctgccggt tgagagccaa ccggcgcgga ttgtccatcc gggacgcgga
       61 agacggcgcc tgcatgatgc tgacgaaaga cggctgccgg atcaatcccg ttaaaccccg
      121 ccagtgccgg gattttccca acaaatggaa ttttcccggc tggcgggatt tgtgccgcgc
      181 cagagaagtg ggggaggaac gggaagcata atatttgccg ggataatatc aaacatatcc
      241 attttctgaa ttgcttccgg cattattcca tggacggggt gaaatccggc tggaatcttg
      301 tgttttttac aggaactgag ccagatcccg ttcaaaaccg ggataggacg tattgatgca
      361 ttccgcatcc gtcactacgg tttctccctg tgcgctgagt ccggccacca ggaagctcat
      421 ggcgatgcgg tggtccccat aactggataa ttccgttccc ttcagggggg tgccgccgtg
      481 aacaaccatg ccatcatcaa attcctccac gtccaccccc atgagccgga gattggcagc
      541 cgtggtggca atgcggtccg tttccttgac gcgcaattcg cgggcgtttc ggacgatgaa
      601 gtctccccgg cccagggctc ccgctacggc caggatgggt atctcgtcaa tgaggttggg
      661 tatttcttcc gggaggaggc tggtgccgtg cagggaatcc gaaccgtaca cggtaatatc
      721 gccgtatggt tcgccggcat cttccggaga agtgggcacg atgtccattc gcgcgcccat
      781 ccgttgcaga gcgctgatga cggcgttccg cgtcttgttc agccccacct ggcgcaacgt
      841 caggcgggac cccggacggc tggcggcggc taccatccag aaggctgcgg aggaaatatc
      901 tgccgggact gtcaaattat gggcgacggg gagagccggc ccgcaggttc cgacggtaag
      961 tccatccacg gtgcagggaa cgccgaaatg gcggaacagg cgttctgtat ggtcgcgggt
     1021 ggcggccggc tggcgcacgg tagtggttcc gtccgcaaac atgcccgcca atagaatggc
     1081 gcttttaacc tgggcgctgg ccatgggaag cgcgtaggaa atagggtgca cccgcccgcc
     1141 gtgaatgctc agcggagcac agcccggttt ggttccccgg gcttcaatcc gcgctcccat
     1201 ttgttccagc ggctgcatga tgcggcccat ggggcgggag ctcagggaag cgtctccaaa
     1261 catttccgaa tcaaaggggc aggcggccag catcccagcc aacagcctca tgcccgtacc
     1321 ggaattgccg cagtcgatgg gccgttcagg agccttgggg ctcatggcga cgcccgtaat
     1381 tcggaagcgg acgggaccgt atccctgccg ttcttccagc acatccactt tggcgcccag
     1441 ctgctccatg gcgcgcaggg tattcaggca atcttcactg catagaaaat tatccacttc
     1501 cgtcactcct tgcgccaggc ctcccagaat ggcggcgcgg tgggaaatgc ttttgtctcc
     1561 gggtacggtc agtacccctt gcagggagga aatggaatgg gaatgaaggt tcatgagttt
     1621 ccggaataac taccctgtat cggctgccga atcaagccaa ttacgagacg ggcggaggca
     1681 tcaggggaca ttccagaatg gagttgattt tccgggacgg tggcgtatat tcatgtgcgt
     1741 tatcggctgc atccccctga aaagaaggag agagcttttc aggatcaatg gatcaagctg
     1801 gttttcatag gtagtaagtt ggagcggcca cgggagcagt cctgtggccg ctttgcctta
     1861 ttcagggaag agggcgccca ttggcttgaa gaaggaagcg gaaggaagaa agaaacaaaa
     1921 ttctttccat gtctgctttg ggctttaccg cgagccgatg aaagcgtatg tagtccatgc
     1981 cgatcaggtt cgggtggtcg ctgttccggt ctccggaccg gagtagagga aagtccggac
     2041 atcacaaggc agcgtgtccc gtgaaagcgg gagacgggaa tctcccaagg gttcccggac
     2101 ggaaagtgcc acagaaaaca aaccgcccgg atggggttct cctcatgccg ggcaagggtg
     2161 aaaaggggag gtaagagctc accgctccga gggtgaccaa ggaggcatgg taaaccccgc
     2221 gcgatgcaag acaaacaggg gaagggtcgc ctgcccgccg gatccccggg tattagtcgc
     2281 atccgtttcg tgaaagcgaa tgggcgcgcg gaacgtctga ccgcggtccg cgtgagaaaa
     2341 atgaccgcac agccggctct gtccggcgga caaaatctgg cttacagaac ctggtcggct
     2401 cctgcttttt taccatgccg ttttccttcc ctcttttcgg gggaagagaa acatccggga
     2461 atttgtcccg ttccgggggc tctttggggt tgcatgggcg ttttaaatgt gtagaatccg
     2521 tcacctaatt gcaacccact atggagaagc ttgtcgtaca tggaggtttt acgctgaggg
     2581 gagccgtcaa tatcagcggg tccaaaaatg cctccctgcc tattttggcc gcatccctgc
     2641 tgacggatga gcctgtagtg gtgcgccggg ttccggatgt ttccgatacc aatttcatgg
     2701 ttcagatcat gggccagctg ggagcctccg tggaacggtc cagcggcaat gtgcgcgtgg
     2761 aagccaggag tctgcattcc gaagccgctt atgaacaggt acgcaagatg cgcgcctcca
     2821 tctgcctgat ggggcccttg atggcccgca tgcagcggtg cgtgattccc ctgccgggcg
     2881 gctgtgtgat tggcgaccgt cctg
//
LOCUS       DAOMSU010000266         2889 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3539_length_2889_cov_2.668666, whole genome shotgun sequence.
ACCESSION   DAOMSU010000266 DAOMSU010000000
VERSION     DAOMSU010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3539_length_2889_cov_2.668666"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="PK077_09340"
     CDS             complement(<1..376)
                     /locus_tag="PK077_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24540.1"
                     /translation="MDSSLTFYLVLGALLLGFIVLGMLKGMIKMLLFGAAVLSSVAAY
                     FWLSKYGFTYLSFITSDPREWMVTVLSVGGAAAVFFVFMHGLFWFSDVFSWGRRMGFG
                     GAKGIVTTVLMAAVVCWVGVMCI"
     gene            complement(409..1473)
                     /gene="aroC"
                     /locus_tag="PK077_09345"
     CDS             complement(409..1473)
                     /gene="aroC"
                     /locus_tag="PK077_09345"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HRN24541.1"
                     /translation="MFRISTWGESHGTGVGVVIDGCPSLVPVTEEDIQRELDRRRPGQ
                     SDIVTPRREEDRAEILSGVLDGKTLGTPIAISVRNKDHRSSAYDEMASTYRPSHADYT
                     YDAKYGIRAWAGGGRASARETIGRVAAGAVARAVLKQAFPDMEVIAWVDQVHHVKASV
                     DWEAVTASVIESNIVRTADSSAAEAMIAAIKEARDSGNSLGGVVKCVVRGCPPGLGDP
                     VFDKLDATLAHAMMSIPATKAFAVGSGFEAAGMTGLEHNDPFYMQGRRVRTATNHSGG
                     IQGGISNGEDILMRIGFKPTATLMIDQQTVNRDGEDARLKGRGRHDACVLPRAVPIVE
                     AMAWLCLCDHYLRQRCQRAL"
     gene            complement(1622..1891)
                     /locus_tag="PK077_09350"
     CDS             complement(1622..1891)
                     /locus_tag="PK077_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HRN24542.1"
                     /translation="MNKAQLIELIQSKLGADTTKKHAEEALAAVLESIKEGVKESGKV
                     QIIGFGTFATKTREARTGRNPKTGNTINIPASKTVAFKASSALKD"
     gene            complement(1973..>2889)
                     /locus_tag="PK077_09355"
     CDS             complement(1973..>2889)
                     /locus_tag="PK077_09355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRN24543.1"
                     /translation="VDLHIRAIQALGAQVQIERGNLIIEAPRGLKGATVDLSGDHGPT
                     VLGTDNLMMAAVLAEGTTVIESAASEPEVVDLANFLIKMGANIQGAGTRRIVIEGVEK
                     LRGCNHTVIPDRIEAGTFMVAAAMMGDGVTLRRVCEEHMTVVTDLLRKCGHHVEFNER
                     GDTVTIIAGKTPKCGEIKTAPYPGYPTDMQAQMTALFATTPGISVVKDTIFPQRFMHC
                     SELKRMGADIKVDNGTAVISGVETLSGAPVMASDLRASAALVLAALKAEGTTEIHRLY
                     HIDRGYEMIDEKLLAIGAAVERLPDDDN"
BASE COUNT          602 a          875 c          830 g          582 t
ORIGIN      
        1 agatgcacat gactcccacc cagcatacga ctgccgccat cagtaccgtc gtgacgattc
       61 ccttggcccc gccgaagccc atgcgccgtc cccaggagaa gacgtcgctg aaccagaaga
      121 gaccgtgcat aaagacaaag aagaccgccg ccgcgcctcc gacggacagc acagtgacca
      181 tccattcccg aggatcgctg gtgatgaaag agaggtaggt gaaaccgtat ttggatagcc
      241 agaagtaagc ggctacggaa gagagcactg ccgcaccgaa caggagcatc ttaatcatcc
      301 ccttcagcat gccgagcacg atgaaaccca gcagcaatgc gcccagaacc aggtaaaatg
      361 tgagggagga atccatgagt gagggaaaag aagccgtgaa accggttgtt acaaagccct
      421 ctggcagcgt tggcgcaggt agtggtcgca caggcagagc caggccatgg cctccacaat
      481 gggcacggcg cgcggcagta cgcaggcgtc atgccgtccc ctgcccttga gccgggcatc
      541 ctccccgtcc ctgttgaccg tctgctggtc aatcatcaag gtggccgtag gcttgaagcc
      601 gatgcgcatc agaatgtcct ccccgttgga gatgcctccc tgaataccgc cggagtggtt
      661 ggtggcggta cgcacccggc ggccctgcat gtaaaaaggg tcattatgtt ccagaccggt
      721 catgcccgcc gcttcaaaac cggaacccac ggcgaaagcc ttggtggcgg gaatgctcat
      781 catggcgtgg gctagcgtag cgtccagctt gtcaaagacc gggtcaccca gcccgggagg
      841 gcagccgcgc accacgcatt tgaccacgcc gcccaaggag tttccggagt cacgagcttc
      901 cttgatggca gcgatcatgg cttccgccgc ggaggaatcc gccgtacgga cgatgttgct
      961 ttcaataacg gaagccgtca cggcctccca gtccacggaa gctttcacat ggtgaacctg
     1021 atccacccag gctatgacct ccatgtcggg aaaagcctgc ttcagtacag ccctggccac
     1081 ggctccggct gcgacgcggc cgatggtttc ccgtgcggag gcccggcccc cccctgccca
     1141 ggcgcgaata ccgtatttag cgtcgtatgt gtagtccgca tgggaaggcc ggtacgtgct
     1201 ggccatctcg tcataggcgg aagagcggtg atccttgttc cgaacactga tggcgatagg
     1261 cgttcccagg gttttgccgt ccagcactcc ggaaaggatt tccgcgcggt cttcctccct
     1321 gcggggggtt acgatgtcgc tttgtcccgg cctgcgccgg tccagctccc gctgaatgtc
     1381 ttcttccgtc accgggacga gggacgggca accatcaatc accacgccta ccccagttcc
     1441 atgggattca ccccaggtag aaattctgaa cacctgacca aaactgctgg acatggcggc
     1501 attctagacg gcggcgttca tccggtcaag ccgtgggcat ccgcagcggg acataacacc
     1561 cctgtcctca aaggacgggg gtgctgaaag aaaagtactc ccttgagaag gagttgccgt
     1621 cttagtcctt caaggcggaa gatgccttga aagcaaccgt cttggaggcg ggaatattga
     1681 tcgtgttgcc ggtcttcggg ttgcggccgg tacgggcttc acgggtcttg gtagcaaaag
     1741 taccaaagcc aatgatctgc accttgccgg attccttcac gccttccttg atggattcca
     1801 gtacagcggc cagagcttct tcagcgtgct tcttggtcgt atcagcaccc agcttgcttt
     1861 gaatcagttc gatcagttga gccttgttca taggacttcg gtgaatagca tatggagccc
     1921 tgacagtaaa gaaaaaatgc ggtatgaagg aaaatcagga cctaaaacgg cgttaattgt
     1981 cgtcatccgg cagcctttct actgcagcgc cgatggccag gagtttttcg tcaatcattt
     2041 catagcctcg gtcaatatgg tacaggcggt ggatttccgt agtgccttcc gccttcaggg
     2101 ctgccagcac cagggcggcg gaggcccgca ggtcggaggc catgacgggg gcgccgctga
     2161 gcgtttccac tccggaaatg acggcggtgc cgttatccac cttgatgtcc gcccccatgc
     2221 gcttgagttc ggagcagtgc atgaaacgct gcgggaaaat agtatccttc accacggaga
     2281 tgcccggcgt ggtggcgaag agagccgtca tctgggcctg catgtccgtg gggtagccgg
     2341 gatacggggc agtcttgatt tctccgcatt tgggcgtttt cccggcaatg atggtgaccg
     2401 tatccccccg ctcgttgaat tccacgtggt ggccgcactt ccggagcaaa tccgtcacca
     2461 cagtcatgtg ttcctcacac acgcgccgca gggtcacgcc atcccccatc atggccgccg
     2521 ccaccataaa ggtgccggcc tcaatgcggt ccgggataac ggtatggttg cagccacgga
     2581 gcttttccac tccctcaatg acgatccggc gcgttcccgc accttgaata ttggcgccca
     2641 tcttgattaa aaagttcgcc agatccacca cttccggttc ggaggctgcg gattcaataa
     2701 cggtggtgcc ttccgccaat acggcggcca tcatcaggtt gtccgttccc agaacggtgg
     2761 ggccgtggtc gccgctcaaa tccaccgtgg ctcctttcaa ccccctggga gcttcaataa
     2821 tcaggttgcc gcgctcaatc tgcacctgtg cccccaatgc ctggatggcc ctgatatgca
     2881 gatccacag
//
LOCUS       DAOMSU010000267         2884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3549_length_2884_cov_3.003888, whole genome shotgun sequence.
ACCESSION   DAOMSU010000267 DAOMSU010000000
VERSION     DAOMSU010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3549_length_2884_cov_3.003888"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1884)
                     /locus_tag="PK077_09360"
     CDS             complement(58..1884)
                     /locus_tag="PK077_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24544.1"
                     /translation="MWDFINTDKAFEIVTSILLSVAIAFTVGTIVMWISRLAFTFKYQ
                     SRFKWFGCFWCGIAMTAIAYFAVFKGLKESTLMEPSFIQYIDQNLGMALLTAFGGFSV
                     LMFLLQHLFMTNILRLTVLAGTFSLALAFAGNDLVNFIGVFMGGLDSFQYASGVAAAG
                     GSPSSITSMECLMPGQGMPVNHYILFAAGCMMIFALWFSKKAKTVIATGVELSRQGEG
                     GIERFGSVPPARAIVRSAIALGRGVKRLIPARMLVRMNHQWDPAPQPLNPKDRVAFDL
                     IRATVNLTVASLLIAWATGRQLPLSTTYVTFMVAMGSSLADKAWGRESAVYRVTGVLT
                     VISGWFMTGLAAFTLAAVMATILHYGQVYAIFGLLALVVVILVKSTIMHRKRESKLSV
                     ERFDQITEENILDVCSHQIVDSTLKLREIYNDTVENFFQGDRKALKELADAAERLAMT
                     SSEHHKYDILPILYKMQSRSLDMGYHFVQALEHLNEATQSLSQFSESIFTYVDNNHTP
                     FTIDQVDDLKEVHTALIKLLDEYCKMLQTGTYIQFDYTMVAQEQLLQLVAKATKRQIK
                     RAQRNQTRTRSSLLYLNMLNEMRFMAVQVRALTNDERNFVAA"
     gene            complement(1868..2296)
                     /locus_tag="PK077_09365"
     CDS             complement(1868..2296)
                     /locus_tag="PK077_09365"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRN24545.1"
                     /translation="MDSIYWIIITALLLLAAFDLINGVANDAVNFLNSAIGSKAAPVR
                     VILTVASIGVLVGACFSGGMMEVARNGIFHPDMFSYHEVMLLFLGVMISEVILLDTFN
                     TFGLPTSTTVPGIRYFGVCRSDGPVQDFQAPPGSVRVGFH"
     gene            complement(2411..>2884)
                     /locus_tag="PK077_09370"
     CDS             complement(2411..>2884)
                     /locus_tag="PK077_09370"
                     /inference="COORDINATES: protein motif:HMM:NF020071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HRN24546.1"
                     /translation="PELELPRFSEEDKLVAISLVCEGAVGYKDIKEREIIPVLRDWLS
                     GWQAKALDDYAPVSLTLPNGQHAKVRYGEDSSPVISLTVQRLFGVAVSPRIANGAVPV
                     IVEVLAPSQRPWQVTGSLESFWRNGYGQMKKDLAGRYPRHRWPDPGELDFLPRSR"
BASE COUNT          700 a          823 c          786 g          575 t
ORIGIN      
        1 cgaattaaac gctcaagaat ttttttcctt acgtgaaaaa aacagagaac cgccgcttca
       61 ggctgctaca aagtttcgtt cgtcattggt cagggcccgc acctgcacgg ccataaacct
      121 catttcatta agcatgttca gataaagcag ggaggaacgg gtgcgcgtct ggttgcgctg
      181 ggcccgcttg atctggcgct tggtggcctt tgccaccagt tgaagaagct gttcctgggc
      241 caccatcgta tagtcgaact gaatgtacgt ccccgtctgg agcattttgc aatattcatc
      301 cagcagcttg atgagggcgg tatgcacctc cttcaaatcg tccacctggt caatcgtaaa
      361 cggcgtgtgg ttattatcca catacgtgaa gatggactcg gaaaactgcg acaggctctg
      421 cgtagcttca ttcagatgct ccagggcctg cacaaagtgg tagcccatat ccagggaacg
      481 ggactgcatc ttgtacagaa tagggagaat atcgtacttg tgatgttcgc tgctggtcat
      541 ggccagccgt tctgcggcat ccgccagttc tttcagggcc ttcctgtccc cctggaaaaa
      601 gttttccacg gtgtcattgt aaatttcccg cagcttgagc gtgctgtcca caatctggtg
      661 gctgcataca tccaggatat tttcttccgt aatctggtca aaacgctcta cggaaagctt
      721 gctttcccgc ttgcggtgca tgatggtgga ctttaccaga atgacaacca ccagggccag
      781 cagaccgaag atggcgtaca cctgtccgta atgcaggatg gtagccatga cggcggccag
      841 cgtaaaggca gccagcccgg tcatgaacca gccggaaatc accgtcagca cgccggttac
      901 acggtaaacc gcgctctccc ttccccatgc cttgtccgcc agggaggaac ccatcgccac
      961 catgaacgtc acgtacgtgg tggaaagagg gagctgcctg ccagtagccc atgcaatcaa
     1021 cagggaagca accgtcaggt tcaccgtggc gcgaatcagg tcaaatgcca cccggtcctt
     1081 gggattcaag ggctgggggg cgggatccca ctggtggttc atgcgcacca gcatgcgggc
     1141 gggaataagc cttttcaccc cgcgtcccag ggcgatggcg ctgcgaacga tggcgcgggc
     1201 gggggggacg gagccgaaac gctcaatacc gccttcaccc tgcctggaaa gttccacgcc
     1261 ggtggcgatc accgtttttg cctttttgga gaaccacaag gcaaaaatca tcatacagcc
     1321 cgcggcgaac aaaatataat ggtttaccgg catgccctgc cccggcatca gacactccat
     1381 ggaggtgatg gatgagggag agccaccggc cgccgccacg ccgctggcgt actggaagga
     1441 atccagacca cccatgaaaa cgccgatgaa attcaccagg tcgttgcccg caaaggccag
     1501 agccagggaa aaagtgcccg ccagaacggt caggcgcaga atattcgtca tgaacaaatg
     1561 ctgaagcagg aacatgagca cggaaaaacc gccgaaggcc gtgagcaggg ccatgcccag
     1621 attctggtca atgtattgga tgaacgaggg ctccatcagg gtagactcct tcaagccctt
     1681 gaaaacggca aaataggcta tcgccgtcat ggcaatgccg caccagaagc agccgaacca
     1741 tttgaaccgg gactggtatt taaacgtgaa cgccagacgg gaaatccaca tgacgatggt
     1801 gcccaccgta aaggcgatgg ctacggaaag aagaatgctg gtgacaatct caaaagcctt
     1861 gtccgtatta atgaaatccc acacggactg aaccaggcgg agcttggaaa tcttgaacag
     1921 ggccgtcgct acggcagacc ccaaaatacc gaataccagg gacggtggtg gacgtaggca
     1981 atccgaacgt attgaaggta tccagcagaa tcacttcgct gatcattacc cccaggaaaa
     2041 gcagcatcac ttcatgataa ctgaacatgt ccgggtgaaa aatgccgttg cgggctactt
     2101 ccatcatgcc tccggagaaa caggccccca ccagcacgcc gatggaagct acggtcagga
     2161 tcacccgcac gggtgcagcc ttggaaccga tggcggaatt gaggaagttg acagcgtcat
     2221 tcgctacgcc gttgatcaag tcaaatgccg ccagaagcag cagggctgtg atgataatcc
     2281 agtaaattga gtccataact gtctcttgtg aatggtgggc ggagggcatc cgcgagctgc
     2341 accaccgcct ctttacacgg aaacggtcga ttttgctact gttttgttac agaaaaatcc
     2401 tttctcctta tcaccgggaa cggggaagga aatccagttc cccggggtcg ggccaacggt
     2461 gccggggata acgccccgcc aggtcctttt tcatttgccc gtatccgttc cgccaaaaac
     2521 tttccagaga tcccgttacc tgccacggcc tctggctggg ggccagcacc tccacaatca
     2581 cgggtacggc cccgttggca atgcgtgggg aaaccgccac gccgaacagg cgctgcaccg
     2641 tcagactgat caccggggag gaatcctccc cgtacctgac cttggcgtgc tggccgttgg
     2701 gcaacgtgag gcttaccggg gcataatcgt ccagcgcctt cgcctgccag ccggaaagcc
     2761 aatccctcag cacgggaata atttcccttt ccttgatgtc cttataaccc acggcccctt
     2821 cacagaccag agaaatggcg accagcttgt cctcctccga aaaccggggc agctccagtt
     2881 ccgg
//
LOCUS       DAOMSU010000268         2883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3551_length_2883_cov_3.085573, whole genome shotgun sequence.
ACCESSION   DAOMSU010000268 DAOMSU010000000
VERSION     DAOMSU010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3551_length_2883_cov_3.085573"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="PK077_09375"
     CDS             complement(<1..638)
                     /locus_tag="PK077_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24547.1"
                     /translation="MTEDAIFKEQWSLLKKEMAMGDAHLQQRLEQFIFRQYGRAVLAH
                     YIKALSALELSRHFYLRFRRRSRSVTEPILYWTVLAETTIHMTLEGVKDMHPFISLPA
                     CAGATFQLDMLKDCYLLYLRDREYPCWPRASRRRRIIRNKWNAPELEASYFPRLCRNS
                     TDRHYANMMHILLEEILHALITLRQLRNHGRSLLEFRRRQHPHSSPGVVPPPS"
     gene            1156..>2883
                     /locus_tag="PK077_09380"
     CDS             1156..>2883
                     /locus_tag="PK077_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRN24548.1"
                     /translation="MDHDFSLILTFVGGLAAALLFGLIARKLHLSPLVGYLLAGVVVG
                     PYSPGFVADSHTVEQFAELGVILLMFGVGLHFHLKDLIAVQKVAVPGAVVQISVATVL
                     GVLVGWMFGWSAISGLVFGMAISVASTVVLTRVLEDNQNLHTPGGHVALGWLVVEDLF
                     TILLLVLIPAVMEARQSGAGDWGGILSELGWMFVKLALLVALTLFAGKKVIPLVLRYV
                     ARTGARDLFTLAVLVIALGVAVYSAEFFGASMVLGAFLAGMVVGQSDFCARAAAEAML
                     MRDAFAVLFFVSVGMMFDPMSVGDCWPLALATLGVVMIGKPLAAYVVVRCLRRPVPLA
                     LSVSVALAQVGEFSFILAGIGLMYNILPPDANQAIILAAVVSISLNPILYRQIGPAVK
                     WLQKRGIGFADPGGVNSMALPAPDARRVVLVGFGPTGRILKRILNDNGVEVIIVEMNI
                     DTVTAIREQGGDIVYGDASQREILRHAGIEYAESLILSASIPDAKEIVGVALELNPHI
                     DVMIHTKYMRDVDILKEAGASQVFSSESEVALSMAEYFLREGGADDEQIVSERQRIRA
                     ELDREMPGVS"
BASE COUNT          574 a          677 c          904 g          728 t
ORIGIN      
        1 gatggcggcg gaacgacgcc cggagaagaa tgggggtgct gtcttcttct gaattccagc
       61 aggctccttc cgtggttcct gagttggcgg agggtaatca aggcatgcag tatttcttcc
      121 agcagaatat gcatcatgtt ggcataatgg cggtccgtgc tgttccggca caaccgaggg
      181 aaataggagg cttccagctc cggagcgttc catttattcc ggatgatgcg tcttctgcgg
      241 gaggccctag gccagcaggg atattcccgg tccctcaaat aaagaaggta gcagtctttc
      301 aacatgtcga gctggaaggt tgcgccggca catgccggaa gggagatgaa ggggtgcatg
      361 tcttttaccc cttccagcgt catgtggatg gttgtttccg caagcacggt ccagtagaga
      421 atgggctccg tgacggaacg gctcctcctg cggaaccgca ggtagaaatg gcggctgagt
      481 tccagggctg aaagggcttt gatataatgc gccaggaccg cccggccgta ttggcggaaa
      541 atgaactgtt ccagcctttg ctggagatgg gcgtctccca ttgccatttc cttcttaaga
      601 aggctccact gttccttgaa tatggcatct tcggtcatag atttgaaaaa atcaattatt
      661 tccaatataa aataaattaa ttttagggaa attcaaatgg aattggggga attttataaa
      721 tttttccaag ttcaggagta tggacgtgga gcatggaaga gcctaagaac aaaagagtct
      781 gcccggcggg cgttcctgct ttggattttt ccgggtcgac ggattatttc cgttgtccgg
      841 cagcaggatg taaaaattgc cgatggtgat gtatgggcat ttttctgaca gccagtgcgg
      901 gacaatgacc cggcagcttc ccgggaatga acaggacgaa tcttactaac gcgtttgata
      961 ggaatcccgt atgggaaaat ttaattagaa gcggtcgcaa atagggtatg atgaaaattt
     1021 gccggggtca ttttcatgca aaaagtgcga ctgcatttcc atgcggcgca atgcgttctt
     1081 gtgagggaaa ataaaacgcg atagcgtaaa aacgacttgg cggtcaagaa taccccttat
     1141 ccctgtcacg cgcgtatgga tcacgatttc agccttattc tcacttttgt cggcggattg
     1201 gccgctgccc ttcttttcgg ccttattgcc agaaaactgc atttatcccc gctggtgggc
     1261 tacctgctgg ccggggtggt ggtagggccg tactcgccgg gatttgttgc ggattcccat
     1321 acggtggaac aatttgcgga gctgggcgtt atcctgctga tgtttggggt ggggctccat
     1381 ttccatttga aggatttgat tgccgtgcaa aaagtggccg tcccgggagc cgttgtgcag
     1441 atatccgtgg ctaccgtgct gggagtgctg gtcgggtgga tgttcggatg gtccgcgatt
     1501 tccggactgg tttttggcat ggcgatttcc gtggcgagta ccgtagtgct gacgcgggtg
     1561 ctggaagaca accagaacct ccatacgccc ggcggccatg tggctctggg atggctggta
     1621 gtggaagacc tgtttaccat tctgctgctg gttctcatcc ccgccgtgat ggaagcgcgc
     1681 cagagcggcg ccggagactg gggcggcatt ctttccgagc ttggctggat gttcgtcaag
     1741 ctggccctgc tggtggccct gacgctgttt gccgggaaaa aagtcattcc gctggtgctg
     1801 cggtacgtgg cccgaacggg cgcgcgtgac ttgtttacgc tggctgtgct ggtcattgcg
     1861 ctgggcgtgg cggtgtactc cgcggaattc tttggcgctt ccatggtttt gggggcgttt
     1921 ttggcgggga tggttgtagg ccagtcggac ttctgcgcgc gcgcggcggc ggaagccatg
     1981 ttgatgaggg atgcttttgc ggttctcttc tttgtttccg tggggatgat gtttgacccg
     2041 atgtccgtgg gggattgctg gcctctggcc ctggcaacgc tgggggtggt gatgatcggc
     2101 aagccgcttg cggcttatgt ggttgttcgt tgcctgagac ggccggtgcc gctggcgctg
     2161 agcgtatccg tggccctggc tcaagtgggt gaattttctt tcattctcgc cggaataggc
     2221 ctcatgtaca acatcctgcc tccggatgcc aatcaggcca tcattctggc ggctgtcgtt
     2281 tccatttcct taaatcccat cctgtaccga cagattggac ctgcagtcaa atggctgcaa
     2341 aaacgcggca tagggtttgc ggacccgggg ggcgtgaaca gcatggcctt acccgctccg
     2401 gatgcgcggc gcgtggtatt ggtagggttt ggccccacgg ggcgcattct taagaggata
     2461 ttgaatgaca acggggtgga agtcatcatc gtggaaatga acatagacac ggttaccgct
     2521 atacgggagc agggagggga tattgtttac ggggatgcca gccagcggga aattctgcgc
     2581 catgcgggca ttgaatatgc ggaaagcctg attctctccg cttccattcc ggacgccaag
     2641 gagatagtag gcgtggctct ggagcttaac cctcatattg acgtgatgat tcacaccaag
     2701 tacatgaggg acgtggatat tctgaaggaa gccggcgctt cccaggtttt ttcctcggaa
     2761 tcggaagtag ccctgtccat ggcggaatat ttcctgcggg aaggcggtgc ggacgacgaa
     2821 cagatcgttt ccgagcgtca gcgcattcgc gcggagctgg acagggaaat gcccggagtt
     2881 tcc
//
LOCUS       DAOMSU010000269         2868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3581_length_2868_cov_2.688944, whole genome shotgun sequence.
ACCESSION   DAOMSU010000269 DAOMSU010000000
VERSION     DAOMSU010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3581_length_2868_cov_2.688944"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..785
                     /locus_tag="PK077_09385"
     CDS             192..785
                     /locus_tag="PK077_09385"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRN24549.1"
                     /translation="MDEAQDMPPAEPDEDAKLMLRVRNGDASAMEMLVRKHQNSVYAT
                     VARMLNNGPETEDIAQQVFIRIWKGAGNYEPSARFTTWMFTILRNLVFNEVRRQKRKP
                     TTSADAMEEEGGMAVFLEPSQTPDEALEHTELQHAVDAAIAALPEKARLAVQLRRFEN
                     MPYEEIARALDMTVPATKSLLFRARNMLKEALASFLS"
     gene            complement(993..>2868)
                     /locus_tag="PK077_09390"
     CDS             complement(993..>2868)
                     /locus_tag="PK077_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HRN24550.1"
                     /translation="GGGKSGEASLRMFGGQDAELKLDLKDTPSREVRLSAWAERWTGQ
                     APFEFSIVAIGPNGEKKIYDGKDIRTGGFRTRIEASVPSGTRSLVFRLTSPENKGMKL
                     DDLFLVPCIPMKVNPQVEMASSIYPVMVRIPCSPVLSLNVRTDGCLNPQFLTAVNLDF
                     TGTTKLSDIESVAVIRGEEAPIIHHGEEPFPKDSSQVFGTVKLAGSARPQISVKGKME
                     LEPGDNYLWACVTMKEGASLDGRVVVRPASVVAGNKPVRVANATPVAQRIGVAVVRHG
                     DFKSKFYRIPGLARSRKGTLLAVYDIRYNHSGDLPANIDVGVSRSTDGGRTWSDVKIA
                     IDDSKIDPSLGATRGVGDPAILVDEKTGRIWVAAIWSHRHSIWGSKSGDNSPEACGQL
                     VLAYSDDDGLTWSSPINITEQTKNKDWRILFNGPGNGICMKDGTLVFAAQYWDGKGVP
                     WSTIVYSKDRGKTWHCGTGVSQQTTEAQVIELEDGSVMINARCNWGGSRIVGVTKDLG
                     QTWEKHPTNRTAQLKEPVCQGSLLAVDGVPGAGRVVLFSNPNTTSGRSHMTLKASTND
                     AGSWPEDKWLLYDARKGWGYSCLAPVDKNHVGVLYESQGALNFLKIPYKDVLNAKNAR
                     "
BASE COUNT          653 a          851 c          754 g          610 t
ORIGIN      
        1 ctcctgcaaa ggcgcgaata tttcccgggc tttggaaaga gtaaaacatt ttacttccat
       61 tccctgcgga aagaaaagaa agacccgttt tcatctagtc agccgtgtaa aaatgggata
      121 gacctttttc cggaatgcgc cctgccggct gcaacctttc accggagacg gggtttataa
      181 aagggaattt catggatgaa gcacaagaca tgccgcctgc ggaaccagac gaagatgcca
      241 agctgatgct gagggtcagg aatggcgacg cttccgccat ggaaatgctg gtccgcaaac
      301 accagaattc cgtatatgcg acggtggccc gtatgctgaa caacggtccg gagacagagg
      361 acatcgccca gcaggtattc atccgcatct ggaagggagc cgggaattat gaaccttccg
      421 cacggtttac cacatggatg ttcaccatcc tgcgcaatct ggtgttcaat gaagtgcgcc
      481 gccagaagcg caagcccacc acctccgcag acgccatgga ggaggaagga ggcatggccg
      541 tgtttctgga accttcccag accccggacg aagcactgga acatacggaa ctccagcacg
      601 ccgtggacgc ggcaatcgcc gctctgccgg aaaaggcgcg gctggccgtc caactgcgcc
      661 gtttcgaaaa catgccctat gaggaaatag cccgggcgct ggatatgacg gttcccgcta
      721 ccaaaagcct gctgttccgc gccagaaaca tgctgaagga ggctcttgct tcctttttat
      781 cctgacaatg gcgtcctcaa aacaagttaa gaacacaccg ccggctttcc cgccaaaggg
      841 tggggagccg gttcctggta cagtttcccg tctttagcgg caaataaccc gtagcgcagc
      901 ggccaagtcc tttcacaaaa acactcggaa acaaaaaaac gggccgttct ccaacgaaaa
      961 cggcccgttg aacatgttac cgggaaacgg aatcagcgcg cattttttgc gttaagaaca
     1021 tccttatagg gaattttcag gaagttcagc gccccctggg attcgtacag cacgcccaca
     1081 tggttcttat ctaccggcgc caggcaggaa tatccccagc ctttgcgggc atcataaaga
     1141 agccatttgt cttccggcca tgacccggca tcattcgtag aagctttcaa cgtcatgtgg
     1201 gaacgtccgg atgtggtatt gggattggaa aacagaacca ctctgcccgc gcccggaacg
     1261 ccgtccacgg caagcagact gccctggcag accggttcct tcagctgggc agtgcggttg
     1321 gtggggtgtt tttcccacgt ttggcccagg tctttcgtaa cgcccacgat gcgggaaccg
     1381 ccccagttgc accgggcgtt gatcatgacg gagccgtctt ccagctcaat cacctgggct
     1441 tccgtcgtct gctggctgac gcccgtgccg cagtgccagg tttttccccg gtctttggaa
     1501 taaacaatgg tggaccacgg cacccctttg ccgtcccagt actgggcggc gaagaccagc
     1561 gtgccgtctt tcatgcaaat gccattgccg ggtccattaa ataaaatgcg ccaatccttg
     1621 ttcttggttt gttccgtgat attgatcgga ctggaccagg tcaggccgtc gtcatcgctg
     1681 taggccagca ccagctgtcc acaggcctcc ggagaattgt ctccggactt gctgccccag
     1741 atggaatgcc tgtggctcca tatggcggcc acccagatgc gccccgtctt ttcatccacc
     1801 agaatggccg gatcccctac gcccctggta gcccccagag aggggtcaat cttggaatca
     1861 tcaatggcga ttttgacatc agaccaggtg cggcctccgt ccgtagagcg gcttacgccc
     1921 acatcaatgt tggccggaag gtctccggaa tggttgtacc ggatatcgta cacggccagc
     1981 agggttccct tcctggaacg ggccagaccg ggaatacggt agaattttga tttgaaatcc
     2041 ccatgcctga ctacggccac gccgatgcgc tgcgccacgg gagtcgcatt ggcaaccctc
     2101 accggtttat tgcccgccac aacgctggcc ggacgcacca ccaccctgcc gtccagggag
     2161 gctccttctt tcatcgtcac gcaagcccac aggtaattgt ctccgggctc cagctccatt
     2221 ttccccttca cagaaatctg gggtctggcg gaaccggcaa gctttactgt accaaaaact
     2281 tgggaagagt ctttcgggaa cggctcttcc ccatgatgga tgataggggc ctcttccccc
     2341 cgtattacag ccacggactc aatgtcggaa agcttcgtcg tacccgtaaa atccagattg
     2401 acagctgtca ggaactgagg attaaggcag ccgtccgtcc ggacattcag agaaagaacg
     2461 gggctgcacg ggatacgcac catcaccggg taaatggaag aggccatctc cacctgcgga
     2521 ttcactttca tgggaataca gggaacaaga aacaggtcgt ccagcttcat tcccttgttt
     2581 tccggagaag taagcctgaa caccagggaa cgcgttccgg aaggaacact ggcttctatc
     2641 ctggtacgaa acccgcccgt cctgatatcc ttgccgtcat aaattttctt ttctccattc
     2701 ggccctatgg ccacaatgga aaattcaaaa ggggcctgcc cggtccatcg ctccgcccag
     2761 gcagaaagcc ggacttccct ggaaggcgta tccttcaagt ccagtttcaa ttcagcatcc
     2821 tgtccgccga acattcgcag ggaggcttct ccggattttc cccctcct
//
LOCUS       DAOMSU010000270         2863 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3593_length_2863_cov_2.761396, whole genome shotgun sequence.
ACCESSION   DAOMSU010000270 DAOMSU010000000
VERSION     DAOMSU010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2863)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3593_length_2863_cov_2.761396"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..371
                     /locus_tag="PK077_09395"
     CDS             138..371
                     /locus_tag="PK077_09395"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HRN24551.1"
                     /translation="MNLQKQQLLLRLCRYFKSRKGEPRFFNKRNICLLAAASAYILTP
                     DEFLFDGIGKLGLLDDAAVILGAVLTVFFPDGD"
     gene            complement(397..1212)
                     /locus_tag="PK077_09400"
     CDS             complement(397..1212)
                     /locus_tag="PK077_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRN24552.1"
                     /translation="MMAFIEPHIHMVCRTTDEYRAMAQAGCLAVGEPAFWAGYDRSSA
                     DGFRDYFVQLTECEPARAAKFGMDHYTWLCINPKEAQDVAFARDVMKLIPEFIDRPNV
                     LGIGEIGLNRNTSHEMTIFEEHLDLAVRLNQLVLIHTPHLEDKLKGTVMILSALKNRP
                     DLDPCRVLVDHCEEHTFPLVKEAGYWAGLTLYPTSKLNPKRAADILELYGMDRVWANS
                     AADWGDSDPLALTALACELRRRGFSRQETERLLLENPETFMGQSPKFRKVSSR"
     gene            complement(1215..1523)
                     /locus_tag="PK077_09405"
     CDS             complement(1215..1523)
                     /locus_tag="PK077_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24553.1"
                     /translation="MTRLSAQAYRLCVKRSKKEVLDADFAAARAGVLDVAAFLDRVDR
                     GEGEADFRYRALMEVLPLLSASGPGRVRAILEALSYRDDTVPETAAAKAACGAPNLSK
                     "
     gene            1688..2389
                     /locus_tag="PK077_09410"
     CDS             1688..2389
                     /locus_tag="PK077_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HRN24554.1"
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIF
                     GGPEWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHAAREPYVFKNT
                     IKATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIV
                     TGFSNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICL
                     GFISVPVSVLLGIIK"
     gene            2411..>2863
                     /locus_tag="PK077_09415"
     CDS             2411..>2863
                     /locus_tag="PK077_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRN24555.1"
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTV
                     LVVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVY
                     RLFYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLD"
BASE COUNT          620 a          895 c          726 g          622 t
ORIGIN      
        1 caggccatcc tctcagcaat tcatttcccc gcatgatccg agtcaccgcg cttccgtccg
       61 ctggaagcga cctctcgccc tgaaacaggg ggaaagaggg gaaatgactg agctttaacc
      121 ttcattcccc gttcctcatg aaccttcaaa aacagcagct gttgctgcgt ctgtgccggt
      181 atttcaaaag cagaaaagga gaaccgcgtt ttttcaacaa acggaatata tgccttctgg
      241 ccgccgccag cgcctacatc ctgactcccg acgaattcct gtttgacgga atcggcaagc
      301 tgggccttct agatgacgcg gcggtcatcc tgggagccgt tctcaccgtc ttttttccgg
      361 atggagacta acggcccgca cccaccgcag gcggaattag cgggacgaca ctttccggaa
      421 cttcggagac tgtcccatga atgtctccgg attttccaag agaagacgtt ccgtttcctg
      481 ccgggaaaaa ccgcgcctgc gtagctcgca ggccagagcc gtcagcgcca ggggatcgga
      541 atccccccag tctgccgcgg aattggccca gacacggtcc attccgtaca attccagaat
      601 atccgccgcc cgcttgggat tcagcttact ggtgggataa agggtcagcc cggcccagta
      661 accggcctcc ttcaccagcg ggaaggtatg ttcctcacag tgatccacca gcacgcggca
      721 agggtccaga tccggacggt ttttcagggc ggaaagaatc attacggtcc ccttcagctt
      781 gtcttccaaa tggggagtgt gaatcagcac aagctggttg aggcgcaccg ccagatccag
      841 atgctcttca aaaatagtca tttcatggga agtattccgg ttcagcccta tttccccgat
      901 gccgaggaca ttgggccggt caatgaactc cggaatcagt ttcatcacat cccgcgcgaa
      961 agccacgtcc tgcgcctcct tgggattgat gcacagccat gtgtaatggt ccatgccgaa
     1021 cttggccgcc ctggcgggtt cgcattccgt aagctgtacg aaataatccc ggaaaccgtc
     1081 cgcggacgaa cggtcgtaac cggcccagaa cgccggttcc cctaccgcca ggcagccggc
     1141 ctgcgccatg gcccggtatt cgtccgtggt gcggcacacc atatgtatat gaggttctat
     1201 gaaagccatc attcttattt gctgaggttc ggggctccgc aggccgcttt ggctgcggcg
     1261 gtttccggca cggtgtcatc cctgtaggaa agggcttcca gaatggcccg gacgcgtccg
     1321 gggccggatg cggacaaaag gggaagaacc tccatcaggg cacggtagcg gaaatccgcc
     1381 tctccttccc cgcggtccac ccggtccaga aatgcggcca catccagaac gccggcgcgc
     1441 gccgctgcga aatccgcgtc cagcacctct tttttactcc ttttcacgca cagacgatac
     1501 gcctgtgcgg aaagcctcgt caagccggaa caggtaacaa aataataact attccaaact
     1561 gataaacagc aacgcctttc cccggctttt caaaaatccg aaaagcgccg gaaattcaag
     1621 aagaggcttg ccatccgggc ttaaaccggg catcctgtga ggacgcattt atttcattag
     1681 agcgcctatg aatgcactag taaaaaccat atgtacattc gtgacatcct ccatcggccg
     1741 caaaatcatt gtggctctga cggggttatg tttggtcttg ttccttgcgg gacacctggc
     1801 aggcaacctt ctcatcttcg gaggccctga atggatcaac acctacgccc acggcctgca
     1861 ctccatgccg gaagcagccc tctggggaat ccgcgcgggg cttgcggtga tcttcatcat
     1921 ccacgtctgg ctgaccatcc agctgaagct ggaaaaccat gcggcccgcg aaccctacgt
     1981 gttcaagaac accattaagg caacgctctc ctcccgctac atgatttaca ccggtttgac
     2041 cgtcctggtg ttcctgatct accacctgta ccagtacacc ctgcgcgtgg gatacgaccc
     2101 cgcccagttc accaccttta tttccgacgg tacggtggaa acctttgacg tgtacaagat
     2161 gatcgtgacc ggattctcca acgtatggtg ctccgccttc tacatcctgg ccgttctgat
     2221 gctgttcagc catctgcgcc acggggtgca gtccatcttc cagactgtgg gcgtcgattc
     2281 ccgcaaaatc cggcccttct acaattttat cgccatcgcc tacggcgcga ttatctgcct
     2341 gggcttcatc tccgtgccgg tctcggttct tctgggtatc atcaaataac catcaactcc
     2401 tctcaaagtc atgattaatt ttcccgtaag cgactggatg ccggatgcca aaattccctc
     2461 cggccctatt caggacaaat ggtccaagta caagcttgaa tccaaactga tcaatccgaa
     2521 caacaagcgc aagtacacgg tgcttgtggt aggttccggc ctggctggcg cctccgctgc
     2581 cgccacgctt gccgaacttg gctacaacgt caaatgcttc tgctatcagg acagcccccg
     2641 ccgcgcccac tccattgcgg cccagggcgg cattaacgcg gccaagaact accagaacga
     2701 cggcgactcc gtttaccgcc tgttttacga caccgttaaa ggcggcgact tccgcgcccg
     2761 tgaagccaac gtttaccgcc tggcggaagt ctcctccaac atcattgacc agtgcgtggc
     2821 ccagggcgtt cccttcggcc gtgaatacgg cggcctgctg gac
//
LOCUS       DAOMSU010000271         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3602_length_2857_cov_2.369379, whole genome shotgun sequence.
ACCESSION   DAOMSU010000271 DAOMSU010000000
VERSION     DAOMSU010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3602_length_2857_cov_2.369379"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="PK077_09420"
     CDS             <1..798
                     /locus_tag="PK077_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24556.1"
                     /translation="LKSLLEQNDLKAFYHHAKTVLSELNAMRRHGTMTREEACDMLWS
                     LYLIGGAPMYEAPDYDSVQPWPYKDERDNDIAAKSRVISALSIMDTKQMSRNLGIHEQ
                     RLKHLHAAYAAAIIKRLKSLHLPDFGKKETALKDEIIRSHPANPDGNVIGSDIKDYEW
                     THRWNNLVTFSSRNSMYHSYVSKIMEKRFIPMLVKYFPDQAGEVVKYIRKAGYGDGEV
                     LDLIDRTAGYNSNTAYLYQGKSGEEHRKKLQQKIRNSCPAASRNANK"
     gene            922..1758
                     /locus_tag="PK077_09425"
     CDS             922..1758
                     /locus_tag="PK077_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24557.1"
                     /translation="MGLFQDQTSSLSEIKRLAALVTDPSRRGEIGSDQWPLAMIAYGL
                     VTCNETGREEEGVIIYNMFQSRCAPDARRKCALQLAAFIRQRQGDGWRALLPFVMADE
                     APDIRRQSAFLIYTLAAPQPEERFPGIAGLANVICASPCPGQASMAPALDALMSLGDM
                     RFAPYLSFISKNLPPGRLADLLTETEAIPTDLGCGWLLDILDEHPELSSTIAAVLAGM
                     PSRAGEVLDVVVPVPSWQFTNSAVQPLHSWSIPEYRLRMEERLSRYLAPEEREAVDRA
                     WS"
     gene            complement(1986..2531)
                     /locus_tag="PK077_09430"
     CDS             complement(1986..2531)
                     /locus_tag="PK077_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN24558.1"
                     /translation="MKSEPLIKRRVAGVDDPLFLDSLDIYRTSFPVHEQRRIQDFPLA
                     FQDPGFCYEVFLNGEGRVVAMLVTWRREKFVYLEYFAVDASLRGQGAGQRILEELRDA
                     FPHKVILEIDPPEDGISRRRLGFYQRHGFVPNPQFDYIHPPYTDEGEPFPLLLMTHGD
                     LLDEETYRSFVDFHHRHVVAR"
BASE COUNT          724 a          838 c          705 g          590 t
ORIGIN      
        1 ctgaaaagcc tgctggaaca aaatgatctg aaagcatttt atcaccacgc taaaacggtt
       61 ctgtcagaac tgaacgccat gcggcgccat ggaacgatga cgcgggaaga ggcctgcgac
      121 atgctctgga gcctttatct gataggcggg gcccccatgt atgaagctcc ggattacgac
      181 agcgttcagc catggcctta taaagacgag cgggacaacg acattgccgc caaatcaaga
      241 gtcatttccg cgctctccat catggatacg aaacagatgt cgcgcaacct gggcatccac
      301 gaacaacggc tgaaacatct ccatgccgct tacgccgccg ccatcattaa aaggctgaaa
      361 tctctgcacc tccctgattt cgggaaaaaa gagacagcgc tgaaagatga aataatacgt
      421 tcccatcctg ccaatccgga cgggaatgtc atcggctctg acattaaaga ttacgaatgg
      481 actcaccgat ggaacaatct cgtcacattt tcgtcaagaa acagcatgta tcatagctat
      541 gtctctaaaa tcatggagaa aagattcatc cccatgctgg tgaagtattt ccccgaccag
      601 gccggggaag tcgtcaaata catcaggaag gcgggatacg gagacggcga ggttctcgac
      661 ctcatcgacc gaacggctgg atacaactcc aacaccgcct atctctacca ggggaaaagc
      721 ggggaggaac acaggaaaaa gcttcagcaa aaaatacgga attcctgtcc agccgcgagc
      781 cggaatgcca ataaataaag gataaaacgc cggtcacact caacaacatg cctcaaccgc
      841 gttcaccgga aaactccgga gtcctgtaag cagtccacgg tttttccttg gcaggtttcc
      901 gcctccggct atcatgcggg catgggcctt tttcaggatc aaaccagctc tttgtcggaa
      961 atcaagcggc ttgccgccct cgtcacggat ccctcccgca ggggggagat cggttcggat
     1021 cagtggcctc tggccatgat cgcctacggg ctggtcacct gcaatgaaac gggaagagaa
     1081 gaagaaggcg tcatcattta caacatgttc caatcccgct gcgctccgga cgcccgccgc
     1141 aaatgcgctc tgcaactggc agcttttatc cggcagcgcc aaggggacgg atggagggct
     1201 ctgctgcctt ttgtcatggc ggatgaggcg ccggatattc gcaggcaatc cgctttcctt
     1261 atttatacgc tggccgcgcc ccagccggag gagcggtttc ccggaattgc cggattggcg
     1321 aacgtcatct gcgcctctcc ctgccccgga caggcatcca tggctccggc gctggacgcc
     1381 ctgatgagcc tgggagacat gcggtttgcc ccttatctgt ctttcatttc caaaaatctc
     1441 cctcccgggc gcctcgcgga cctgctgacc gaaacggaag ccattccgac agacctgggc
     1501 tgcggatggc tgctggatat tctggatgaa catccggagc tttcctccac cattgcggcg
     1561 gtactggccg ggatgccctc cagagccgga gaagtactgg acgtggtcgt gcccgtcccc
     1621 tcctggcaat tcaccaattc cgccgtccag ccgctccatt cctggagtat tcccgaatac
     1681 aggctgcgca tggaagagcg gctctccaga tatcttgccc cggaagagag ggaagccgtg
     1741 gaccgggcat ggagctgagt cttccggtat atcccaaccc gctgcatttt tcaaaaagcc
     1801 gtcccccaaa aatgcggaac ggctcttctg attcggaaaa tacaggctaa ggggagttcc
     1861 acaaacaggc aaaacgcaat atgagcctga tgtcctacag gctcatatcg gcaaaagccc
     1921 cccaaggata atttgcttca agaggggaaa atcccccatg gcgcggattt tccccggcca
     1981 ttcattcacc tcgccactac gtggcgatgg tggaaatcca caaagctccg gtatgtctcc
     2041 tcatccagca aatctccatg cgtcatcagc aggagcggga aaggttcacc ctcatccgta
     2101 taaggaggat ggatataatc aaactgcgga ttggggacaa agccgtgcct ctgataaaac
     2161 cccagccgcc ggcgtgagat cccgtcctcc ggaggatcaa tttccaaaat aaccttgtgc
     2221 ggaaacgcat ccctcaattc ttccagaatc ctttgcccgg cgccctggcc gcgcagggag
     2281 gcatccacgg caaaatactc cagatacacg aatttttccc gacgccacgt caccagcatc
     2341 gcaaccaccc ggccttcccc atttaaaaat acttcataac aaaaaccggg gtcttgaaac
     2401 gccaacggga aatcctgtat ccgccgctgt tcatgcaccg gaaagcttgt ccggtaaata
     2461 tccagagaat ccaggaacag cggatcatcc acccccgcca cacggcgctt gatcagcggc
     2521 tcagacttca tggaccatat ccaaccacac ccgtctcctt tcggcaagtc ctccggaaag
     2581 tcttccccag tcatacaacc agttgttcag gcttcaattg atgcagtgct tcccaatcaa
     2641 ggataaggaa gaaaaatacc ttcgcgcggc gatacggaga caaggaaatc cccgtctgcc
     2701 tgccgggatt gacggcagcc ccggcgcctg tacaagggat gtcgaaacaa aggcattcca
     2761 tgttctcttt tatctctatg aatttacttt tacctgatgt atagaaagag cctgctcacg
     2821 tcatgaattc catttgaaat tttattccgg aaacatg
//
LOCUS       DAOMSU010000272         2857 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3603_length_2857_cov_2.254104, whole genome shotgun sequence.
ACCESSION   DAOMSU010000272 DAOMSU010000000
VERSION     DAOMSU010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2857
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3603_length_2857_cov_2.254104"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PK077_09435"
     CDS             <1..906
                     /locus_tag="PK077_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24559.1"
                     /translation="APDELRLKAGKGIEIIETENEREQMIAALIEDAKEPSSGQCSLI
                     YEEKEDGGNSEGNQGGDNGGDQGGQGENKGEPYKLKLLCSSDGSVNIKDEEGKLSFSA
                     QKVEPGDGLEWKKEKDQDGNDIDTQILQVKIDSTEADSPKPGKWPVNLSVSPEGLKGE
                     LDLTVDTSVHDLGGGASVGLSNATAGVLSLVVTPGGDAEELSFRAPLRKNGKYVVLDY
                     VKEPHTLPDGTTIALGLLGTQLDLVVDTSNTTGGGDGAMISDSWTALACDTDHALRLH
                     RDENGQIYIQQGQWITTSQIYSPIN"
     gene            913..1695
                     /locus_tag="PK077_09440"
     CDS             913..1695
                     /locus_tag="PK077_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24560.1"
                     /translation="MNYAIFCYREDHQCLGLCLEQIRSIDRAAQFYLFDDAAKPLFPA
                     QVPAGNDISYKITYFARRGNLNGLECVRGMLGCMLDIPGDDPVIKIDADTLLMDPAEI
                     IRSLKDRGKVAGGMQCSVPLAWAGCCYWLTRPAIKAALELLARREWPENARQEYPEDE
                     TISKILLYLYGASGVDVLEFRGGRRLIGVRTCDPRDLEEIARLARGGVCAVHCGQMAF
                     YGPLAEREGISIRDACARIMEAISHSRNNKHSLSANLPRTSR"
     gene            1716..>2549
                     /locus_tag="PK077_09445"
     CDS             1716..>2549
                     /locus_tag="PK077_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRN24561.1"
                     /translation="MTSFRDSSSFLPAGCREIPQAPGPEKGVLALLAMDPTTYMSQAV
                     AFGVTEDFFHIPAHQLLWRLFLARYNANLPLDIISVTQALADGNQLDAVGGNAGLAEI
                     YTFTTTGAYFEHYLRTLRDKSLLRSIIDIANQSTTQAFDNPEDVAALLDAVETHIFQI
                     RERSRSRADGQSLASLVRDAVTNFEKFIASKGRIQGLSTGFDQLDKKSNGLKPGDMFV
                     IAARPSMGKTSFLLNIMEHVALNEKKPTLLFSCEMPAVQIVERLLFARSGVRRGEIVR
                     RG"
     assembly_gap    2550..2559
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2564..>2857
                     /locus_tag="PK077_09450"
     CDS             2564..>2857
                     /locus_tag="PK077_09450"
                     /inference="COORDINATES: protein motif:HMM:NF015736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRN24562.1"
                     /translation="MQHFKRAVEDVGASQLVIDDTAAISITELRAKARRVMRDQGGLA
                     VIGVDYLQLMRSTSRQAANSREREVAEISAGLKSLAKELKVPVVVLAQLNRGPE"
BASE COUNT          647 a          749 c          859 g          592 t
ORIGIN      
        1 gcccctgatg aattgaggtt gaaagccggt aaggggatag agattataga gacggaaaat
       61 gaaagggagc agatgatcgc agctctgatt gaggacgcga aagagccatc cagcggacaa
      121 tgctctttga tttacgaaga aaaggaagac ggcgggaact ccgaaggaaa tcagggaggc
      181 gataatggag gggatcaggg agggcaaggc gaaaacaagg gagaacctta caagctgaaa
      241 ctgttatgtt cttctgacgg ttcggtcaac attaaggatg aagaaggaaa actgtcattt
      301 tccgcccaaa aagtggaacc tggggatggc cttgaatgga aaaaggaaaa ggatcaggac
      361 gggaatgaca ttgatacgca gattttacag gtcaagattg attcaacgga ggcagattct
      421 cccaagccgg ggaaatggcc tgtaaacttg tccgtctctc ctgagggatt gaagggggaa
      481 cttgatttaa cggtagatac cagcgttcat gatttaggtg gaggggcttc cgtgggattg
      541 tccaatgcta cggcgggggt attgtccctc gtggtcactc ctggaggcga cgcggaagaa
      601 ttgagttttc gcgccccttt gcggaaaaat gggaagtatg ttgtgctgga ttatgtcaag
      661 gagccacaca ccttgccgga cggaacaacg attgccttgg ggttattagg cacccagctt
      721 gatttggtgg tagatacgtc caacacgacc ggcggcgggg acggagccat gatcagcgat
      781 tcctggacag cgttggcctg cgacactgac cacgccttac gcctgcaccg ggacgaaaac
      841 ggacaaatct atatccagca ggggcaatgg attacaacat cccaaatata ttcaccaatc
      901 aactaaacaa caatgaacta cgccatattt tgctatcgag aagatcacca atgcctgggg
      961 ctgtgtctgg aacagatacg aagcattgac cgggccgccc agttttattt atttgatgat
     1021 gccgcgaagc ctttatttcc ggcacaagtc cccgcgggaa acgatatatc ctacaaaatc
     1081 acctattttg cgcgccgggg gaatttgaac ggcctggaat gcgtgcgcgg catgctgggg
     1141 tgcatgctgg acataccggg ggatgatccg gttatcaaga ttgacgcgga tacgttgctg
     1201 atggacccgg cggagattat acggtccctg aaagaccgcg ggaaagtagc ggggggaatg
     1261 cagtgcagcg tgccgcttgc ctgggccggc tgctgctact ggctgacgcg cccagccatc
     1321 aaggccgcgc tggaactgct tgcccggcgg gaatggccgg aaaacgcccg tcaggaatat
     1381 ccggaagatg aaaccatttc aaaaattctg ttatacctgt acggggcgag cggcgttgac
     1441 gtgctggagt tccggggcgg gcggcgtctg attggcgttc ggacgtgtga tccgcgcgat
     1501 ctggaggaaa tcgcccgcct ggcgcgcggc ggcgtgtgcg cggttcattg cggccagatg
     1561 gctttttacg gtccgctggc cgaacgggag ggcatttcta tccgggacgc ctgcgcacgc
     1621 attatggagg caatttctca ttcgagaaat aacaagcatt ccctttccgc caatcttccg
     1681 cggacctccc gctaaccctc cgccgacact aagcaatgac ttctttccgc gattcttctt
     1741 ccttcctgcc tgccggatgc cgggagattc cccaggctcc cggcccggaa aagggcgttc
     1801 tggccctgct ggcaatggac ccgacgacct acatgtccca ggccgtcgcg ttcggcgtga
     1861 cggaggattt tttccatatc ccggcgcacc agttgctgtg gcggttgttc cttgcccggt
     1921 acaacgccaa tttgccgctg gatattattt ccgtgacgca ggcccttgcg gacggtaacc
     1981 agctggacgc cgtaggaggc aacgccgggc tggcggaaat ctacaccttt accaccaccg
     2041 gagcgtattt tgagcattat ctccgtaccc tgcgcgacaa gtccctcttg cggtccatca
     2101 ttgacatcgc caaccagtcc actacgcagg cctttgacaa cccggaggac gtagcggccc
     2161 tcctggacgc cgtggaaact catattttcc agatacggga gcggtccagg agccgcgcgg
     2221 acggccaaag tctggcttcc ctcgtccggg acgccgtcac caactttgaa aagttcatcg
     2281 ccagcaaggg gcgcattcag ggactttcca cgggttttga ccagctggac aagaagagta
     2341 acggcctcaa gccgggggac atgttcgtga ttgcggcccg cccatctatg gggaaaacgt
     2401 cttttttgct taatatcatg gagcatgtcg cgctcaatga gaagaagccg acccttcttt
     2461 tttcctgcga aatgcccgcc gtccagattg tggagcgtct tcttttcgcc cgttccggcg
     2521 tccgccgcgg ggagattgtc cggcgaggcn nnnnnnnnna gagatgcagc atttcaagcg
     2581 agccgtggag gacgtggggg cgtcccagct ggtcattgac gataccgcgg ccatttccat
     2641 caccgagcta cgggccaaag cccgccgcgt catgagggac cagggagggc tggccgtcat
     2701 cggcgtggat tatctgcaac tgatgcgttc cacgtccaga caggcggcca acagccggga
     2761 acgggaagtg gcggaaatat ccgcgggcct gaaatccctg gcgaaggaac tcaaggttcc
     2821 cgtcgtcgtg ctggcccagc tgaaccgcgg accggag
//
LOCUS       DAOMSU010000273         2856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3606_length_2856_cov_3.058194, whole genome shotgun sequence.
ACCESSION   DAOMSU010000273 DAOMSU010000000
VERSION     DAOMSU010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3606_length_2856_cov_3.058194"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PK077_09455"
     CDS             complement(<1..897)
                     /locus_tag="PK077_09455"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRN24563.1"
                     /translation="MADLQKHVHEVASRVTAATVALVSDGGETGSGVIVSPQGLILTA
                     AHVVGGDEIMRVVFADGRVVKGRVLGANFTRDAAMVQIMDGGNYPHVELGESDGLHVG
                     DFVVALGHSKGFDPERRAPIRMGRLCTDGKQRFLISECTLIGGDSGGPLFDLSGKLVG
                     IHSSIGPMLKINNHVPVSVFRRDWDKLLSGRHWGQLGLHPMADPESPVLGFAMMDVLG
                     VDGVVVEDVVVNSPADTAGIQPGDVITHMDSRSLRSVRDMLRELGRHRPGETVPLVVV
                     RKGTAYKADLTFGRRGDLMSGLK"
     gene            1211..>2856
                     /locus_tag="PK077_09460"
     CDS             1211..>2856
                     /locus_tag="PK077_09460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRN24564.1"
                     /translation="MSSRSQTERKKRPLPRPSYPGLPQENSDPAVIPGPTRRTGNSRN
                     GPSRYDTPPQRRASRPSPKEEYEENPEREEENRPARAGTMRRPRFTIFGALLFVLLMP
                     FRFIGSLTRNIRWFICWPLRILLGCCFVGIVIGAILVFLYGTISNRYDISEVKSNIPE
                     RTVILDRKNRTIGTLHGENRKRVSLQEVPPVFIDALLLREDNRFYDHGGVDWIGVGRA
                     FAQVLKHKRATQGASTLTMQLAKITYNHRERNLHSKLTEVALAKRIEATYTKDEILET
                     YINRIFWGHTFLGIAAAARGYYDKEPRDLSLAECATLAGIIYGPNDFSPIKHPEEAKK
                     VRDIVLGLLKNEGKITEVQYQAALAEPIATRTPQSRSEENYAMGLVRRELDAILEEED
                     IRLGGLVVHTTLDLDLQNATLDAINKHMDALEARKDFKKHLASLQARREKRGILKNKL
                     TTKAEYEASLAAWKALPAEQKEKTQPPMPNYIQSAAVVMDNATGALLAVVGGRDAEES
                     KLNRAIQSRRQTGSLFKPFIYATFFQQGNSTDTRISDDRIS"
BASE COUNT          683 a          889 c          747 g          537 t
ORIGIN      
        1 tttcaggccg gacatcaggt cgccgcgcct gccgaatgta agatccgctt tgtaagccgt
       61 acctttcctc accaccacca atggaacggt ttcccccgga cggtgcctgc ccagttcacg
      121 gagcatatcc cgcacggaac gcaggctccg gctgtccatg tgggtaatga cgtcccccgg
      181 ctggatgccg gcggtatccg ccggagaatt caccacaacg tcttccacca ccacgccgtc
      241 cacgcccagc acatccatca tggcaaaccc gagcacagga gattcggggt ccgccatggg
      301 atgaaggccc agctggcccc aatgcctgcc ggagagcagt ttgtcccagt ctctcctgaa
      361 aacggaaaca gggacatggt tgttgatttt caacataggg ccaatggagg aatggatgcc
      421 aaccaacttg ccggacaggt caaagagggg accgccggaa tctccgccaa tcagcgtaca
      481 ttccgaaata aggaagcgct gctttccgtc cgtgcacaat cttcccatgc ggatgggcgc
      541 gcggcgttcc gggtcaaatc ccttggaatg gcccagagcc accacaaaat cccccacatg
      601 aagaccgtcg gattccccca gttccacatg gggatagttt cctccgtcca tgatttgcac
      661 catggcggca tcccgcgtga agttggcgcc cagcacccgt ccctttacca cgcgcccgtc
      721 ggcaaagacc acacgcatga tttcatcgcc gcccacgacg tgggccgccg tcaaaatcag
      781 gccctgcgga gagacaatga cgccgctgcc cgtctctccc ccgtcggaaa ccagagccac
      841 cgtagcagcc gtaaccctgg aggccacctc atgcacatgt ttctgcaaat ccgccatgga
      901 agaaacattc cccgcaaggc tcgccccccc tgacagcagg gacacaagcc ccccaacgac
      961 caagcaacaa aaactcgtca ttctcaatag tgatatcatg aaattaccga taatcgaatg
     1021 ataggacata ccctactgta taaacgttca aaacggcaga attttgtcta tggattgcga
     1081 catggcgccc catcttttgc ttagaccatt tcgattactt atgataaagt ccggctcacg
     1141 gatgggttta aacgacaaat caaggaaacg aaatgccgcc tcctggaggg aacgcatcca
     1201 agatggagaa atgtcttccc gttcccagac agaacgcaaa aaaaggcccc ttccccgtcc
     1261 atcctatccc gggttgccgc aggaaaattc tgatccggcc gtcattcccg gccctacccg
     1321 cagaacggga aattcccgca acggcccttc ccggtatgac acgccgcccc agcgacgggc
     1381 ctcccgcccg tctccgaagg aagagtacga agaaaatccg gaaagagaag aagaaaaccg
     1441 tcccgcacgc gcaggaacca tgcgcaggcc gcgtttcacg attttcggag ccctgctgtt
     1501 tgtcctgctg atgccgttcc gcttcatcgg ctccctgacg cggaatatcc gctggttcat
     1561 ttgctggccc ctgcgcatcc ttctgggatg ctgcttcgtc ggcatcgtga tcggagccat
     1621 cctggtattt ctttacggca ccatttccaa ccgctatgac atttccgagg taaagagcaa
     1681 cattccggaa cgcacggtga ttctggaccg taaaaaccgg accatcggaa ccctgcacgg
     1741 ggaaaaccgc aagcgggtat cccttcagga agttcctccc gtttttattg atgccctgct
     1801 cctgcgcgaa gacaaccgct tttacgacca tggcggcgtg gactggatag gcgtgggccg
     1861 cgcctttgcg caggtgctca agcacaagag agccacgcag ggggcttcca ccctgacgat
     1921 gcagcttgct aaaattacat ataaccaccg ggagcgcaac ctccattcca aactgacgga
     1981 agtggccctg gcgaaacgca ttgaagccac ttatacgaag gacgaaattc tggagactta
     2041 catcaaccgt attttctggg ggcacacctt cttggggata gcggcggcgg cgcggggata
     2101 ttacgacaag gaaccccggg atttatccct ggcggaatgc gccaccctgg ccggcattat
     2161 ttacggccca aatgattttt cccccatcaa acatccggag gaagcaaaaa aagtgcgcga
     2221 tattgtcctt ggcctgctga aaaacgaagg caagattacg gaagtgcagt atcaggcagc
     2281 cctggcggag cctatcgcca cccggacgcc ccagtcccgg tctgaggaaa attacgccat
     2341 gggtctcgtg cgccgggagc tggacgccat tctggaggaa gaagacatcc gcctgggagg
     2401 gctggtggtg cataccacat tggaccttga tctgcagaac gccacgctgg acgccatcaa
     2461 taagcacatg gacgcgctgg aagcccgcaa ggatttcaag aagcacctgg cctccctgca
     2521 ggccaggcgg gagaagaggg gaatcctgaa aaacaagctg accaccaagg cggaatatga
     2581 ggcctccctg gccgcctgga aggcgctccc tgcggaacag aaggaaaaga cgcagccgcc
     2641 gatgcccaat tacatccaat ccgccgccgt agtcatggac aatgccacgg gggctcttct
     2701 ggccgtcgtc ggcggccggg atgcggaaga atccaagctg aaccgcgcca ttcagtccag
     2761 gcggcagacc ggctccctgt tcaaaccttt catttacgcc acgtttttcc agcagggcaa
     2821 ttctacggat acccgcattt cggatgacag gatttc
//
LOCUS       DAOMSU010000274         2845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3621_length_2845_cov_2.276344, whole genome shotgun sequence.
ACCESSION   DAOMSU010000274 DAOMSU010000000
VERSION     DAOMSU010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3621_length_2845_cov_2.276344"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..489)
                     /locus_tag="PK077_09465"
     CDS             complement(46..489)
                     /locus_tag="PK077_09465"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HRN24565.1"
                     /translation="MSSPWSPYMPDRDRPDPHASPVRYDYLSRDEQEVRQALADWFQI
                     SPATSETRNLHTAESLLGDILSKLPLDGDGMDPELLREGWLKAAGPFLGQQSNLLSIV
                     KGVATIQVLQPAMRYHLQQWQGALLGKLKDQFGKDAVHSIRIRIG"
     gene            complement(562..795)
                     /locus_tag="PK077_09470"
     CDS             complement(562..795)
                     /locus_tag="PK077_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009959880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRN24566.1"
                     /translation="MADIDEKTPLNVPGKFYVDSSCIDCDLCRETAPENFGRDDDEGV
                     SYVKKQPDNDEELSACEEALEGCPVEAIGDDGE"
     assembly_gap    1078..1087
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1088..2831
                     /gene="lnt"
                     /locus_tag="PK077_09475"
     CDS             <1088..2831
                     /gene="lnt"
                     /locus_tag="PK077_09475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HRN24567.1"
                     /translation="TALWYGRRREGKRGILAYALYGWVFGVVFYGISFWWVNEVSTLG
                     YIPLMIFYGGLFPGLWALVMGVFFRPDARPLPDAKLAAKERRAAWKAWAMGDILPSAS
                     AALAGAALWVCLEWGRGWLIPGFGWNNLGVALYGNPLAQWAEYFGVTALAFIPAVFSV
                     WLWRVCRRAGTMIVHEGRRTVPWDFFILVAVLLTMFVVGVCWTARYSPQSAAVTGNGR
                     LTVPVMAVQLNLSQKEKWDPANRGSIYRALLDMTEQGMLDLQNRALEQAVKNGAEASL
                     DMPAWVIWPESSFPISTFYRDSTGERFPNQDNVNFLSAEEDYVQALRNGICNFILLTG
                     TDDIYLSDEGRVARAYNCLTVFEGDYSTARPHAKAMLVPFGEYIPMRETFPFLEKAFE
                     ASAGTAMGLNYTPGRSSSPVPVPIRPGSSSTVGVIPLVCFEDVVGSWVRRFIRQEPQL
                     MVNVTNDGWFNRSCANEQHWRNAAFRCIELRRSMVRAANTGVSVALAPNGAVIADLRD
                     SSGSPFTRGVMAATLPVGCTEITLYAMLGDWAVLVCFLVFAALLLRRIGAGKRIHGPV
                     ENGVYRRSTGATPR"
BASE COUNT          516 a          766 c          863 g          690 t
ORIGIN      
        1 ggtaagtatc aggcgttttg gggaaaatgt cgttcggcgg caaaattatc ctatccgtat
       61 gcggatggaa tggacggcgt ccttgccgaa ctggtctttc agtttcccca gcagggctcc
      121 ctgccactgc tggagatggt agcgcatggc cggttgaagc acctgaatgg tggccactcc
      181 cttgacaatg gaaagaaggt tggattgctg gcccaggaat ggccccgcag ctttcagcca
      241 tccttcccgg agcaattcgg gatccatccc gtcgccgtcc aggggaagct tggacagaat
      301 atcccccagc agggattccg ccgtatggag gttacgtgtt tcactggtgg cgggggatat
      361 ctggaaccag tccgccagcg cctgcctcac ctcctgttcg tcacgggaaa gataatcata
      421 ccggacgggg gaagcgtggg gatccggacg gtctctgtcc ggcatgtaag gagaccaggg
      481 agaggacatg ggtggaaaat aaaagcccgg agcgttgaat accaacagcc ccgggcggtt
      541 aaaataatat atgggggaat attattcgcc gtcgtcgcca atcgcttcca cggggcagcc
      601 ttccagggct tcctcacagg cgctcagctc ttcgtcgttg tccggctgct tcttgacata
      661 ggatactcct tcatcgtcat cccgtccaaa gttttccgga gccgtttcgc ggcaaaggtc
      721 acagtcaatg caggaactgt ccacgtaaaa tttgccgggg acgtttagag gtgttttttc
      781 gtcgatatct gccattgtgt atgatgggtt gtatgaactg cgcccactat ttccgcattt
      841 gagtaaattt tgcaatcttt tttgttgcag gaatgagtca gcatggggga caatggcggc
      901 attcccgttc ccggaataaa aggcttttca cgatgaatat cccacctgct tccctccctc
      961 gtctcctgcc cgtgtgggca gggctgctgc tggccgcttt ttccggaatt ctgatggcct
     1021 gcgcctttat tccggtagac tggagcgggt gcgtatggat aggattcctg cctttgcnnn
     1081 nnnnnnngac agccctgtgg tacggccgca gacgggaggg gaaaagggga attctggcgt
     1141 acgccctgta tggttgggta tttggcgtgg tattttacgg catctccttc tggtgggtca
     1201 atgaagtcag cacgctgggc tatattcccc tgatgatttt ttatggaggc ctgtttcccg
     1261 gcctctgggc tctggtcatg ggagtgttct tccgtccgga cgcccggccg ctgcctgatg
     1321 cgaagctggc ggcaaaggaa cgtcgggccg cctggaaagc ctgggccatg ggcgacatat
     1381 tgcccagtgc ttccgccgct ctggcgggcg ccgctttatg ggtatgcctg gaatgggggc
     1441 ggggttggct gataccgggt ttcggctgga acaatctggg agtggccctg tacggaaatc
     1501 cgctggccca atgggcggaa tactttggcg tgacggcgct ggcctttatt ccggccgtct
     1561 tttccgtctg gctctggcgt gtttgccgcc gggccggaac catgattgtc catgaaggca
     1621 gacgtaccgt tccctgggac tttttcatcc tggtggcggt tcttttaacc atgtttgtcg
     1681 tcggcgtgtg ctggacggct cgctactccc cccagtctgc cgcggttacg ggaaacggca
     1741 ggcttaccgt tcccgtcatg gccgtgcagc tgaatctgag ccagaaggaa aaatgggatc
     1801 cggccaaccg gggaagcatt taccgggctt tgctggacat gacggaacag ggaatgctgg
     1861 atcttcaaaa ccgcgctctg gaacaggcgg tcaaaaacgg cgcggaagcc tccctggata
     1921 tgccggcatg ggtcatctgg ccggaaagtt ccttccccat ttccacgttt taccgtgatt
     1981 ccacagggga acgcttcccc aatcaggaca atgtcaactt tctgagcgcg gaagaggatt
     2041 acgtccaggc cctccggaac gggatatgca attttatcct gctgacgggc acggatgata
     2101 tttacctgtc tgatgagggg cgtgtggcgc gggcttacaa ctgccttacc gtctttgagg
     2161 gggattattc cacagcccgt ccgcacgcca aggccatgct ggttcccttc ggtgaatata
     2221 tccctatgcg ggaaacattc ccgtttctgg aaaaagcctt tgaggcttcc gccggaacag
     2281 ccatgggcct gaattatacg cccgggcgtt cctccagccc cgttcccgtg cctatccgcc
     2341 ccggaagctc ctctacggtg ggcgttattc cgctggtctg ctttgaggat gtggtgggaa
     2401 gctgggtgcg ccgcttcatc cggcaggagc cccagttgat ggtgaacgtg accaatgacg
     2461 ggtggttcaa ccgttcctgc gccaatgaac agcattggcg caatgcagcg ttccgctgta
     2521 ttgaactgcg ccgttccatg gttcgtgccg ccaataccgg cgtaagcgtg gctctggccc
     2581 ccaatggcgc ggttattgcg gatttgcggg attcctccgg ttctccgttt accagagggg
     2641 tgatggctgc cacattgcct gtgggctgta cggaaataac cctgtacgcc atgctgggcg
     2701 actgggccgt cctggtttgt ttcctggtgt tcgcggctct gttgctgcgc aggataggcg
     2761 cagggaaacg catccatggc cccgtggaga acggcgttta ccggcgttcc acgggggcta
     2821 cgcccagata attcctgacc tgttt
//
LOCUS       DAOMSU010000275         2828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3651_length_2828_cov_2.808150, whole genome shotgun sequence.
ACCESSION   DAOMSU010000275 DAOMSU010000000
VERSION     DAOMSU010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3651_length_2828_cov_2.808150"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            245..1045
                     /locus_tag="PK077_09480"
     CDS             245..1045
                     /locus_tag="PK077_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN24568.1"
                     /translation="MQFHVATMIGRAALRLVDRLGSIGLLLYQVASCMWSGQFRMRVL
                     VEQIAKIGVQSQPVVIITGAFTGAVLEAQTLFQLQTVRMETMGGAVVAVGMFRELGPV
                     ITGLMLAGRVGSAMAAEIGTMKVTEQVDALNSMNVDPVDYLVKPRIQAMLISMPILMM
                     EAVLVGIASAYIVSVTAFNVDQAYWMHFMSKFVTMGDIIVALVKALAFGLIISTISCR
                     EGLNTTNGAVGVGKSTMQAMVYASVALLIANFILTMILNSFFPMGFMR"
     gene            1061..1879
                     /locus_tag="PK077_09485"
     CDS             1061..1879
                     /locus_tag="PK077_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRN24569.1"
                     /translation="MQPFIRVHQLRQSFGTQEVLKGVSFDVGKGELMALIGGSGAGKS
                     VILKHLDGLMEPLDGYVEIDGRRISGAPEKIKKQIRSKIGFMFQQGALFDSLSVGENV
                     AFPLQEAGIRDENELDTRISAALDSVGLLGQQEKMPSSLSGGMIKRVAVARAIITTPE
                     CLLYDEPTAGLDPIVTDSISFLIRRICKDKGITTVIVSHDMPSVIRIADKIVYLRNGE
                     VYWTGTPEELLHSKDEILQKFLYGDSGENWAALSGKNENFQRVLLERAERERKQ"
     gene            1898..>2828
                     /locus_tag="PK077_09490"
     CDS             1898..>2828
                     /locus_tag="PK077_09490"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HRN24570.1"
                     /translation="MKQKLKPETWVGLFLIAGILMIIGVILGFGNIKTSKEQTYPINI
                     IFKDAAGLIKDSQIRLGGVTVGKVTKAPELLPSGNEVMLEASIQSDVKIQQGSVFRVD
                     MQNILGDKYIDIVPPAQPTHEYIPPHATIIGQPESDFSKIKNNAVGATEEILKILKQI
                     EKNSDNIDVAILNIGEAAKGLAQTTRLINEGILNRENLQNLGSVLSQINRTGEQLPGL
                     MEETRSSVAAMKDTVRDARKLIAGAEEKLNTLDPAVKAIPPTLAALRKASEQISSFTA
                     DARKNQGFLGLLMYDARFRANAQEFIRNLRDYGI"
BASE COUNT          699 a          833 c          754 g          542 t
ORIGIN      
        1 cgccacggag ctggcgaacc ggttcccgga atcggaaagg gaaatacgcc gtttgcagcg
       61 gcaggcccgc gcatccgaat ggacgcgcct gaaactgccg ccccgcctca tgctgcgctc
      121 catccccgga acgcaggaaa cgccttatat ccatcggctg gcagactgac gcggaacctt
      181 ccttttcctc ccgtggcata cattgcgctt gctcttgaag cggaagaaat gattagtaga
      241 agccatgcaa ttccatgtag caaccatgat cggccgcgcc gctttaaggc tggttgaccg
      301 gttggggtca atcggattgc tgctttatca agtggcttca tgcatgtggt ccggccagtt
      361 ccggatgcgc gtgcttgtgg agcaaattgc gaaaatcggg gtgcaatccc agccggtggt
      421 catcatcacc ggcgcgttca ccggcgcggt gctggaagcc cagacactgt tccagcttca
      481 aacggtgaga atggaaacca tgggcggggc cgtcgtcgcc gtgggcatgt tccgggagct
      541 ggggcccgtc atcaccgggc tgatgcttgc ggggcgcgtc gggtccgcca tggccgcgga
      601 aatcggcacc atgaaagtca cggaacaggt ggatgccctc aactccatga atgtggatcc
      661 ggtagactac ctggtgaagc cgcggataca ggccatgctt atttccatgc ccattctgat
      721 gatggaagcc gttctggtgg gcattgcctc cgcatacatc gtcagcgtca ccgcttttaa
      781 cgtagaccag gcctactgga tgcacttcat gagcaaattc gtcaccatgg gagacatcat
      841 cgtcgccctg gtcaaggctc ttgctttcgg cctcatcatt tccaccatct cctgccggga
      901 ggggctgaac actacaaacg gcgccgtggg cgtaggcaaa tccaccatgc aggccatggt
      961 gtacgcttcc gtggccctgc tcatcgccaa ctttatcctg accatgatcc tcaactcgtt
     1021 cttccccatg ggattcatga gatagccttt ccccgccgcc atgcagccat tcatccgcgt
     1081 tcaccagctc agacaaagct tcggcaccca ggaagtgctg aaaggcgtaa gctttgacgt
     1141 gggcaaagga gaactgatgg ctttaatagg aggctccgga gccgggaaaa gcgtcattct
     1201 gaaacatctg gacggcctga tggaaccctt ggacggttat gtggaaatag acggcaggcg
     1261 catcagcggc gcgccggaaa aaatcaaaaa gcaaatccgc agcaaaatcg gattcatgtt
     1321 ccagcagggg gccttattcg actccctaag cgtgggagaa aacgtagctt tccccctgca
     1381 ggaggcgggg atcagggatg aaaacgaact ggacacccgc atctcagccg ctttggactc
     1441 cgtagggctg ctgggacagc aggaaaaaat gccgtccagc ctctccggcg gcatgatcaa
     1501 gcgcgtcgcc gtagccaggg caatcattac cactccggaa tgcctgctct atgacgaacc
     1561 caccgcgggc ctggacccca tcgtcacgga cagcatcagc ttcctcatcc ggcggatctg
     1621 caaggacaaa ggcatcacca ccgtcattgt ctcccatgac atgcccagcg tgatccgcat
     1681 cgcggacaaa atcgtctatc tcagaaacgg tgaggtttac tggacgggaa ctccggaaga
     1741 acttctgcac tccaaagacg aaatcctgca aaaattcctg tacggggact ccggggaaaa
     1801 ctgggctgcc ctgtccggca aaaatgaaaa tttccaacgg gttctgctgg aacgggcgga
     1861 acgggaacgc aaacaataac catcttgcca cagagccatg aaacagaaac ttaaaccgga
     1921 aacatgggta ggcctctttc tgatcgccgg aatcctgatg attatcggag tcatcctggg
     1981 attcggcaac atcaaaacat ccaaggagca aacctacccc atcaacatca ttttcaaaga
     2041 tgcggcggga ctcatcaagg actcccagat ccgtctgggc ggcgtcacgg tggggaaagt
     2101 caccaaagcc cctgaactcc tgccttccgg caatgaagtc atgctggaag ccagcatcca
     2161 gagcgacgtg aaaatccagc aagggtccgt cttccgggtg gacatgcaga acatcttggg
     2221 cgacaaatac atcgatatcg tccctccggc ccagcccacg catgaataca tccctcccca
     2281 tgccaccatc atcggccagc cggaaagcga tttcagcaaa atcaaaaaca atgccgtggg
     2341 cgccacagaa gaaatcctga aaatcctcaa gcaaatcgaa aaaaattcgg ataacattga
     2401 cgtcgctatc ctcaacatcg gagaggcggc caaagggctg gcccagacga ccaggctgat
     2461 caatgagggc atcctgaacc gggagaacct ccaaaacctc ggcagcgtac tgtctcaaat
     2521 aaaccggacg ggagaacagc tccccggcct catggaagaa acgcgctcct cggtcgcagc
     2581 catgaaagac accgtccggg atgcccgcaa actcattgcc ggagctgaag aaaaactcaa
     2641 cactctggat cccgccgtca aggcaatccc ccctacgctg gccgctctga ggaaagcatc
     2701 tgaacaaatc tcctccttca cggctgatgc acgtaaaaac cagggctttc ttgggctgct
     2761 tatgtacgat gctcgcttcc gggccaacgc tcaggaattc atccgtaatc tgagggatta
     2821 cggcattc
//
LOCUS       DAOMSU010000276         2820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3666_length_2820_cov_2.322242, whole genome shotgun sequence.
ACCESSION   DAOMSU010000276 DAOMSU010000000
VERSION     DAOMSU010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3666_length_2820_cov_2.322242"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            224..1507
                     /locus_tag="PK077_09495"
                     /pseudo
     CDS             224..1507
                     /locus_tag="PK077_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024126.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
     gene            complement(1563..2750)
                     /locus_tag="PK077_09500"
     CDS             complement(1563..2750)
                     /locus_tag="PK077_09500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HRN24571.1"
                     /translation="MSLPLADDEEKGKRFSCGCPEYISPSRNYGIDALRIVAMMMVLI
                     LHLLASIDVLSLENHGSASYNVGWLLEIAAYCGVNCYALITGYVCCDGTFRYERVVSL
                     WFQVVFYTWGSLLLALLFFPQEVQLSNILHSLFPVLSGQYWYVTAYVGLFFFIPFLNA
                     LGNRLTKLQFQYLLVTVFVLFSVIPTLLHTDVFPVEEGYSIWWLGILYMLGMYVKKHG
                     LLTGMKTRPLWMFYAGCVCFAWVFKMVLNVVSPYLIGQIKGGGMFIRYNSPFIVGTAV
                     ALLLIFSRMHFSSRRAVSCISWLAAASFSVYVLHCNALIGKWFLWDVFEWAASSSSAL
                     MVVNVLAIAAVVYAGCALVDSVRRYLFKLMNVERGARAVTGFCGKLGHAFRKMCRRID
                     SHP"
BASE COUNT          777 a          863 c          641 g          539 t
ORIGIN      
        1 cccggcaaga tggactgccg cccccatgcc gaagccggaa gccaggccca ggccgcatac
       61 ccgcggttcc gggcttccaa gactcttacc ccccgccatc atgacggcgg ccagactacc
      121 gccctgcccg gaaaaactca agcttccgat gcgtcaggaa caacctgaaa agaccaccgc
      181 tgcaaccttg caatggaagc ttcttttatg gcaatctggc ttcatgacta ttcagcccgc
      241 cgtttttttc tctcttctat tggcatccct ttctcttccc tccttctccc ttaccccgcc
      301 cagtgaaaaa ttccgggcgg cagactgggc ggtgcttcat tcatcccgga gcgaccagcg
      361 gcatgtttcc tcccggggca tcgtccagac catgctggaa aaactgcgtc cggcatgcga
      421 attttccccg gatatggctc cgaaggattt ccccgcctgg cgggaaaaag tccggaaagc
      481 catgcaacaa ctgatgaaat tcccccccca ggcggatatc cccgcccccg ttctggtaaa
      541 aaccgtgcca agagaacact accgggtgga aaaatgggaa gcctatccct tcccgggagc
      601 cgccgtaccg ttcctggtgc tcctgccgaa caacgcttca gacaaaaatc cggttcccgt
      661 gctattctgc attcccgggt cagaccagac caaggaagag ctggcggggg aaacatcacc
      721 ggatctggac cagccttccg tacagcagcc gggcaacaac gccatggctt tccattatgt
      781 caggcaggga tgggcggcca ttgtggtgga caatgccgga acgggtgaag aaggagacgc
      841 ggaacacgcc gcaggccgtt cttcacacga ttatgaaaac ctggcccgct tcctgctgga
      901 aatggactgg agctggctgg gctacacctc ctacgcggac caatgtattc tggattgggt
      961 gaaaacacgc ccctgggcgc aaaaaaacca tattatcctc agcggcttct ccctgggaac
     1021 ggaacccatg atggttctgg gcagcctgaa cccggatatc tttgccttcg tgtataacga
     1081 ctttctgtgc cgcacgctgg aacgcgccaa aaccatgacc atgcccaacg gcaggggcgt
     1141 gcgttccgct cccaactcca tccgccacct cattccgggt ttctggaaac aattcgactt
     1201 cccggacatc gtggcggctc tcgctccgcg ccctgtcatt tgcacggaag gcggcctgga
     1261 cagggacttc cagctcatct ccgcagccta ccgtatggcc ggggcgccgg atcatttcca
     1321 gtaccatccc cagccccggt ttgcggaccc tgcctaacgc tggcacggca gccaattgcc
     1381 cgcaggtctg gacagggacg cctttttccg tttcgccaat gtggacccgc gcaaccattt
     1441 cttcaaaaaa gacctggtca ttccctggct taaaaccctc ctggataaaa gcgataataa
     1501 aaactagccc agctccagac aaaaaccgcc cggaaccgcc ggaacaggac attccctcat
     1561 ccttagggat gcgaatctat ccggcggcac atcttccgga atgcatgccc cagctttccg
     1621 caaaagcccg tcaccgcccg ggcgcccctt tccacgttca tgagcttaaa taaataacgc
     1681 cgcacggagt ccaccaaggc gcatccggca taaaccacgg cggctattgc caacacgttc
     1741 acaaccatca gggctgagga agaagacgct gcccactcaa agacatccca caaaaaccat
     1801 tttcctatca aagcattaca atgcagcaca taaacgctga acgacgctgc cgccagccat
     1861 gaaatacagg agacagccct tcgggatgaa aaatgcattc gggaaaaaat cagcaacagg
     1921 gcaaccgccg tccctacaat aaaaggagaa ttatagcgaa taaacattcc accgcccttg
     1981 atctgcccaa tcaggtatgg agacaccaca ttaagaacca tcttgaaaac ccaggcaaaa
     2041 catacgcatc cggcataaaa catccataac ggacgcgttt tcattcccgt cagcaagcca
     2101 tgctttttaa catacatccc cagcatgtaa aggatgccca gccaccagat gctataccct
     2161 tcttccaccg ggaacacatc cgtgtgaagc agggtgggaa taacggaaaa cagcacgaaa
     2221 acggtaacca gaagatactg gaactgtaat ttggtcagcc tgtttcccag ggcgttaaga
     2281 aacggaatga agaaaaacag ccccacatac gccgtcacat accaatactg gccggataaa
     2341 acgggaaaaa gggaatgcag aatattactc aactgaacct cctgggggaa aaacaacaga
     2401 gccagcagca aactgcccca tgtataaaaa acaacctgaa accataagga aaccacacgt
     2461 tcatacctga acgttccgtc gcagcacaca tatcctgtaa ttaacgcata acaattcaca
     2521 ccgcaataag cggcaatctc cagcagccac ccgacattat aggaagcgga cccatggttc
     2581 tccaaagaca aaacatcaat ggaggccaaa agatgaagaa ttaaaaccat catcatggcg
     2641 acaatgcgca aggcatctat tccataattc cgggacgggg aaatatattc cggacaacca
     2701 caggaaaaac gctttccttt ctcttcatca tcagccagcg gcaaactcat ggagaaacat
     2761 ttctacggat ttattatccg cgttcaagcc ttaatattga agaagaataa tttaagaaaa
//
LOCUS       DAOMSU010000277         2816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3676_length_2816_cov_2.171677, whole genome shotgun sequence.
ACCESSION   DAOMSU010000277 DAOMSU010000000
VERSION     DAOMSU010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3676_length_2816_cov_2.171677"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..1035
                     /gene="zupT"
                     /locus_tag="PK077_09505"
     CDS             223..1035
                     /gene="zupT"
                     /locus_tag="PK077_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HRN24572.1"
                     /translation="MDLSADHILVVFLLTTLAGLATGIGGFIAFFMKRTDTKALTFAL
                     GLSGGVMVYISLVELLGEAQHRLMEFEGHTAGSWIAIASFFGGIAVAALIDYLVPEDE
                     NPHEARGPEDIHGQASGEFSSSRIKRSGILFALAIGIHNFPEGIATFAAGLDSLTLGT
                     SIALAVAVHNIPEGIAVAVPLYYGTGSRKKALFYSFLSGLAEPVGAAIAMFFLFHFLT
                     PTVLAVLFASVAGIMVFISFDELLPMAERWGHHHISIMGIIAGMLLMAIVLI"
     gene            1131..2282
                     /locus_tag="PK077_09510"
     CDS             1131..2282
                     /locus_tag="PK077_09510"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HRN24573.1"
                     /translation="MPESPASPASPASPASPASPASSAVSSVPPTSTQASSLLSPLSC
                     WKKGFLHYADFRGCASRAEFWWFMALPLLALIPALAGYILTDWLHIPDTRLSIYGDAL
                     TILLWAVLFIPSISAAFRRLHDTGRSGLWLFSLFIPFGLGHLIFFYLTLGESKADGNK
                     YSRRPEPQPADPPARELKEQPLTPFYLYWLISLRKLNTVAGRASRTEFWSFFLLSVLL
                     FLPLGYSMIDVDSQPAGFYVSPSLQILLYAAHPQDALILLAHSCFNPTFYFFYQSGEL
                     SMLSLELLAAVAGLNILFNLPVAVRRLHDSNLSGKFILIPILIFIVTFLLIFLLRLVP
                     EDMAPYLDYLGMVSSLMDLLSILFLSMMLLKSSPGPNEYGVLPQKITVS"
     gene            complement(2357..>2816)
                     /locus_tag="PK077_09515"
     CDS             complement(2357..>2816)
                     /locus_tag="PK077_09515"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRN24574.1"
                     /translation="IPFSLENVVECIKARKAAGQREILVVVSEGARLADELVLLDEKT
                     QGEVRLGGIGKVIAKKLEEATGIETRSCVLGHIQRGGSPCSYDRILGTRFGSYAVELV
                     EKRQFSCMVALRGTQMTAVPIEEAVKTLKLVDPDCQLVRTARDLGVCFGD"
BASE COUNT          542 a          898 c          679 g          697 t
ORIGIN      
        1 cgttgcgctc cttccggaaa gttttgaagg aaaagcgttc ggcaaacttc tggacaagct
       61 gcctgaaatc aagaaggaac ggggcctcct gaagccgggg gaagagcccc cccttccccc
      121 cggcgccatc cgggccacga agatgatcgt tcccacggct cccgcaaaat aatacacccc
      181 tttctttact gccggggtca tccctgcccg gcaggtgcac gcatggacct ttccgccgac
      241 cacatcctcg ttgtcttcct gctcaccacg ctggctggtc ttgctaccgg cataggaggc
      301 ttcatcgcct tcttcatgaa aaggacggat acaaaggcac ttacttttgc cctgggatta
      361 tccggcggag tcatggtgta tatctccctg gtggaactgc tgggagaagc ccagcacagg
      421 ttgatggagt tcgaaggcca tacagcaggt tcctggatcg ccattgcttc cttcttcggg
      481 ggaattgccg tggcggctct gattgattac ctggttccgg aagacgaaaa cccgcacgaa
      541 gcgcgcggac cggaagacat ccatggacag gccagcggtg aattttcctc atcccgaatt
      601 aaaaggtccg gcattctgtt cgccctggcc atcggcatcc acaatttccc ggaaggaatc
      661 gccacctttg ccgccggatt ggactccctg actttaggca cctccatcgc cctggccgta
      721 gccgtccata acattccgga aggaatcgcc gtggcggtac ctctttacta cggtacgggc
      781 agccgtaaaa aggccctttt ttattccttt ctctccggtc tggcggaacc ggtgggcgcg
      841 gccatcgcca tgttcttcct gttccacttc ctcacaccta ccgtacttgc ggttctcttt
      901 gcctccgtcg cgggaatcat ggtcttcatc tcctttgacg agctgctgcc catggcggaa
      961 cgctggggac accatcacat ttccatcatg ggcatcatcg ccggaatgct gctgatggcg
     1021 attgtcctga tctgaaccgg ctgcaggagg gaactttcca tgtcctgggc acagacgcat
     1081 tgagcattcc ccggcatcag ggagtctaaa cggaaacccc cttttcatcc atgccggaat
     1141 ctcctgcttc tcctgcttct cctgcttctc ctgcttctcc tgcttctcct gcttcttctg
     1201 ctgtttcctc cgttcctcca acctccacgc aggcatcttc cctcctttcc cccctctcct
     1261 gctggaaaaa aggttttctt cattatgcgg actttcgggg ctgcgcttcc cgtgcggaat
     1321 tctggtggtt catggctctt cccctcctgg cgctgattcc agccctggcg gggtatatcc
     1381 tgacggactg gctgcacatc cctgatacaa gactgagcat ctacggagac gccttaacca
     1441 tcctcctgtg ggcggtgtta ttcatcccaa gcatatcagc cgcgttcaga cgcctgcatg
     1501 atacaggcag gagcggcctc tggctttttt cccttttcat tcccttcggg ctggggcatc
     1561 tgatcttttt ttatctgacg ctaggagaaa gcaaggcgga cggcaacaaa tacagccgcc
     1621 gtccggagcc ccaaccggct gatccccctg ccagagaact gaaagagcag ccattgactc
     1681 cgttttacct ttactggctc atcagcctgc ggaaattgaa tacggtggca ggccgcgcgt
     1741 cccggacgga attctggtcc tttttcctcc tttccgtcct cctgttcctt ccgctgggct
     1801 acagcatgat agacgttgac agccagccgg cgggttttta tgtgtctcct tccctccaaa
     1861 tcctgttata tgccgcccat ccgcaagatg ctctgatcct gctggctcac tcctgcttca
     1921 atcccacctt ttactttttc taccaatccg gagagctgag catgctttcc ctggagcttc
     1981 tggcagccgt ggcggggctc aatatcctct tcaatctgcc ggtcgccgtg cgccgcctgc
     2041 atgacagcaa tctgagcgga aaattcatcc tgattcccat tcttattttc atcgtcactt
     2101 tcctgctgat tttcctgctg cgcctggtcc cggaggacat ggccccctat ctggactacc
     2161 tgggaatggt gtccagcctg atggatctgc tttccatcct cttcctgtcc atgatgcttc
     2221 ttaaaagctc gccaggcccc aatgaatacg gcgtgcttcc gcaaaaaata accgtatcct
     2281 gattccagga tacggttgaa aagcaatcaa aaccctatcg ggtacaatca atttcccgtg
     2341 acgcagacgg agaacgtcag tccccgaagc atacgcccaa atcgcgggcc gtgcgcacca
     2401 gctgacaatc gggatccacc agcttcaagg ttttcacagc ttcctcaatg ggtacggcag
     2461 tcatctgcgt cccacggaga gcaaccatgc agctgaattg gcgcttttcc accagttcca
     2521 cggcataact gccgaaccgc gttcccagaa tccggtcata ggaacaggga gaaccgccgc
     2581 gctggatatg gcccagcacg caggagcggg tttcaatccc agtggcttct tccagtttct
     2641 tggcaatcac cttgccgatg ccacccaggc gaacttcccc ctgggtcttt tcatccagca
     2701 ataccagttc gtccgccaga cgggcccctt cagataccac caccaggatt tcccgctgcc
     2761 cggcagcctt gcgcgccttg atgcattcca ccacgttttc aagggaaaaa ggaatt
//
LOCUS       DAOMSU010000278         2803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3699_length_2803_cov_3.249636, whole genome shotgun sequence.
ACCESSION   DAOMSU010000278 DAOMSU010000000
VERSION     DAOMSU010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3699_length_2803_cov_3.249636"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PK077_09520"
     CDS             complement(<1..651)
                     /locus_tag="PK077_09520"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HRN24575.1"
                     /translation="MMNSVFKCLMSAVCAVALPAFGQEEKTGFPTDRAVTVFSAGEGN
                     PYASIRIPALLSIGKGQLLAFAEGRYKNTDQGENDIIMSVSKNGGKTWSRPRAIAKAH
                     GATFNNPCPVYDARTKTVTVVFQRYPAGVKERQPNIPDGWDDEKCIRNFMIQSRNGGS
                     SWTKPQEITKTTKRPSGVDIMASGPNAGTQLKSGAHKGRLVIPMNEGPFGKWVISCI"
     gene            1056..2576
                     /locus_tag="PK077_09525"
     CDS             1056..2576
                     /locus_tag="PK077_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HRN24576.1"
                     /translation="MNRILPLLSLPLLSLAAAFAANTPEHIGNDLKLFKDSSCTSLKP
                     DVKDTSAFQSDAMKELAAKILAGHYKPDYLYAEYRALPSPRQTGKNLRIGDGFSKYDN
                     MTGVYLEKGRHVVLVGKTEGQEISLLLPNLMRKPAEGVQPTKDPNGWGLHKKQIPLKE
                     GINIIDVETPANAYISYFTEDAGKAPKIPVHFVTGKANGYFDTTRGDTNKDWVRLLDQ
                     AVSPIMDARGKYIQVAYPVEFLKKFTKDRGTELINAYDKLIGIQYQLMGLDKYGKIPE
                     NRVLARVNFNYYMFRDGDGVAYLGNDGTMRMVTDPENVLKGDACWGFSHEVGHVMQMR
                     PMTWGGMTEVSNNIFSLQAAAKTGNESRLKRQGSYDKARKEIIEGEIAYLQSKDVFNK
                     LVPLWQLHLYFTKNGHPDFYPDVMEYLRNNAGNYGGNDTVKYQFEFVKACCDVTKTDL
                     TDFFEKWGFFKPGKFHIGDYAQYDFNVTPEMAAETKKWIAGKGYPKPETDITELSE"
BASE COUNT          750 a          838 c          677 g          538 t
ORIGIN      
        1 aatgcaggaa atcacccatt tgccgaacgg cccttcattc atcggaatca ccaggcggcc
       61 cttgtgggcg ccactcttca gctgggttcc cgcattcggg ccggacgcca taatatccac
      121 tccggaagga cgcttggtcg tcttcgtaat ctcctgcggc ttcgtccagg aagaacctcc
      181 gttcctgctc tgaatcatga aattgcggat gcacttttca tcatcccatc cgtccgggat
      241 attgggctgc cgctccttga cgccggcagg gtaacgctgg aacacgacag tcacggtttt
      301 ggttctggcg tcataaacag ggcacggatt attgaacgtg gcgccatggg ccttggcaat
      361 cgcccgggga cgggaccagg tcttcccgcc attcttgctg acgctcatga taatatcgtt
      421 ctccccctgg tcggtattct tgtaccgtcc ttcggcgaat gccaggagct ggcccttgcc
      481 gatactgagc agggcgggaa tacggatgga cgcataggga ttgccctccc cggcgctgaa
      541 tacggtcaca gccctgtccg tagggaaacc ggttttctct tcctgcccga acgccggcaa
      601 tgccacggcg catacggcac tcatcaaaca cttgaatacg gagttcatca tcctctccac
      661 cttattccat tttccccttc cgcacaacaa ccatgtcatg catgtgcgtc aagaccagag
      721 aaaaaggaac ataaagagaa acacgcggca ggaacgcaat actgccgccg ggaagcccgg
      781 aaggcttcgg ttcaaaactc aagaggggaa caaggaacgc gcccatccgg atacggcgcc
      841 ccggaaaagc gcccgccgga acacgggaaa tatccggaaa cgtgaaaccg gccgaaaaaa
      901 acctgccgca acgccaaaac cattccacaa atcaaccgcc cggcctttct cctgacggac
      961 tgcctattta ttcatcaaga gaaaaatggt gaaagaagaa gaaaacggaa cctgtatctt
     1021 gtaacatcac ggaaccacct gcttcccgta ctgtcatgaa ccgaatcctt ccattactat
     1081 ccctgcccct gctgtcgctc gcagcggcat ttgccgccaa cacgccggaa cacatcggca
     1141 acgacctcaa actgttcaaa gactcctcct gcacctccct gaagccggat gtcaaagaca
     1201 cttccgcctt ccagtctgac gcgatgaagg aactcgcggc caaaatcctg gcgggccatt
     1261 acaagccgga ctacctgtat gccgaatacc gggccctccc ctcaccgcgc cagacgggga
     1321 aaaatctcag aatcggcgac ggattcagca aatacgacaa catgacgggc gtctatctgg
     1381 aaaaggggcg gcatgttgtc ctggtcggca agacggaggg gcaggaaatc agcctcctgc
     1441 tgccgaacct gatgcgcaag ccggccgaag gagtgcagcc cacaaaagac cccaatggct
     1501 ggggattgca caaaaagcag attccgctca aggaaggaat caacatcatt gacgtggaaa
     1561 cgcccgccaa cgcctatatc agctacttca ccgaagacgc cggcaaggcg ccgaaaatcc
     1621 ccgtccactt cgtgaccggc aaagccaacg gctactttga caccacccgg ggcgacacca
     1681 acaaggactg ggttcgcctg cttgaccagg ccgtctctcc gatcatggat gcccggggaa
     1741 aatacatcca ggttgcctac cccgtagaat tcctgaaaaa attcaccaaa gaccgcggaa
     1801 ccgaactcat caacgcctac gacaagctca tcggcatcca ataccagctg atgggcctgg
     1861 ataaatacgg caaaatcccg gaaaaccgcg tcctggcccg cgtgaacttc aactactaca
     1921 tgttccgcga cggagacgga gtcgcctacc ttggaaacga cgggacaatg cgtatggtca
     1981 ccgaccctga aaacgttctc aagggcgatg cctgctgggg attctcccac gaagtaggac
     2041 acgtcatgca aatgcgcccg atgacctggg gcggcatgac ggaagtcagc aacaacatct
     2101 tttccctgca ggccgcagcc aaaacaggca atgaaagccg cctgaagcgc cagggcagct
     2161 acgacaaggc ccgtaaggaa atcatcgaag gggaaatcgc ctacctgcaa tccaaggacg
     2221 tcttcaacaa gctggttccc ctgtggcagc tccatcttta ctttaccaaa aacggacacc
     2281 ccgacttcta tcctgacgtc atggagtacc tgcgcaacaa cgcgggaaac tacggaggga
     2341 acgacaccgt caaataccag ttcgaattcg tcaaggcatg ctgtgatgtc acaaaaaccg
     2401 acctgacgga cttctttgag aaatggggat tcttcaagcc cggaaaattc cacatcggcg
     2461 actatgccca atacgacttt aacgtcaccc ccgaaatggc ggcggaaacg aaaaaatgga
     2521 ttgccggcaa aggctacccg aaacctgaaa ccgacatcac cgaattaagc gagtaacgag
     2581 attcggcaat cccgacgatt ccttttaatc atcaaatatg ctgcaaacgg ataagaaaac
     2641 aggggctcaa gttcaattct tttgaaagta tttttgatga tggaagatta ttaagaagtt
     2701 aaactccctc accaaagggt ggaatcattt ctgattcctc cctttttcat ttctatatcc
     2761 atccccctat tcccagccat actgagtacg acaaatgtca ggt
//
LOCUS       DAOMSU010000279         2801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3703_length_2801_cov_2.450109, whole genome shotgun sequence.
ACCESSION   DAOMSU010000279 DAOMSU010000000
VERSION     DAOMSU010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3703_length_2801_cov_2.450109"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="PK077_09530"
     CDS             <1..520
                     /locus_tag="PK077_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HRN24577.1"
                     /translation="HGSDGAPVPDLASVLSACPGPESRGVYKCHITYDTRGRVRRTSF
                     EPYRPRQVKTLTCVEMPGLDYSCKWEDRTGLLAAGAALGCDEEALILRNGMVTDTRYS
                     NVVFGDGSSWVTPETFLLPGTKRAFLLSRGDIQECSVRAEDIGKFRFCSLINAMLDPG
                     DVVVRTEDIILC"
     gene            complement(644..1825)
                     /locus_tag="PK077_09535"
     CDS             complement(644..1825)
                     /locus_tag="PK077_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HRN24578.1"
                     /translation="MAATLDMPSLGKFYRRQLLEDVLPFWFPRAYDEKNGGLYHCFDA
                     DGTLVDSDKSVWAQGRMAWMLLTMYNSIEKNTDWLKWAESALEFLKTKCVDPADGRMF
                     FHVAADGTPIRKRRYAYSESFAAIAFAAHARATGSRDSAREARHWFDIFTDNCFTPGK
                     MVPKFTGERPTTGLGTRMITLNTAQEMRKYLGDDDGFYTGWTDRCINDLRTLFMKPDI
                     QAVMEVVGTDGSIIDHFDERTLNPGHTTEGGWFVLEEARHRGNDPELIKVGCDMIDWA
                     FARGWDKENGGMLYYTDVYNKPVQEYWHNMKFWWPHDEALIAMTLAYKLTGEERYAIR
                     HDMVRNWAFSHFQDVQHGDWFGYLNKDGSRANTLKGSLWKSFFHHPRAMWCCAHYCGA
                     I"
     gene            complement(1845..2762)
                     /locus_tag="PK077_09540"
     CDS             complement(1845..2762)
                     /locus_tag="PK077_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HRN24579.1"
                     /translation="MDTSLKLHGLVAAVHTPFKADGSLNPSSVDAQAKLLASQGIKLA
                     FITGSTGESSSMQLEERKEIYSAWKEASAKHGVEVIAHTGSNSVWDARELASFAQECG
                     FVATSSLAPSYYKPGTVQCLVECCAFAASGAPDLPYYYYDIPVLTGVRFNPVDFIRLA
                     KEQIPNFAGIKFTNPDLALYQTTLNYDETVDIPWGVDEWFTGALSVGAKGAVGSSFNF
                     APALYQKLMKAFAEGDVETARDCQWKSVQMINILASKGYMGCAKALMGWLGVDLGPAR
                     LPQGNPTADQLKELRSELEGIGFFQWALN"
BASE COUNT          562 a          735 c          883 g          621 t
ORIGIN      
        1 tcatggttcg gacggagctc ccgttcccga tctggcttcc gttctttcgg cctgtcctgg
       61 cccggagagc cgtggagttt acaaatgcca tatcacgtat gatacgcggg gaagggtgcg
      121 ccgcacctcc tttgagccct accgtccccg gcaggtgaag acgctgacgt gtgtggaaat
      181 gcccgggctg gattactcat gcaagtggga ggatcggaca gggcttctgg cggcaggggc
      241 agccttgggc tgtgacgaag aagcgctgat tctccggaac gggatggtga cggatacgcg
      301 gtacagcaac gtggtgttcg gggacgggtc ctcctgggtt actccggaaa ctttcctgct
      361 cccgggaacc aagcgcgcct tcctgctgtc ccgcggagac attcaggaat gttccgtaag
      421 agcggaagat ataggcaaat tccgcttctg ttccctgatt aatgccatgc tggatcccgg
      481 cgatgtggtg gtgcggacgg aggatattat cctgtgttga gggtggaaaa acggatagat
      541 tgttttatcc ggaaccctct gaattttggt attgagggca taaaaaagcg gccgttccat
      601 ggatatggaa tgaccgcctg acgggaaaaa gcctgacgcc ttcttaaatg gcgccgcagt
      661 agtgggcgca gcaccacatg gcgcggggat ggtggaagaa ggatttccag aggcttccct
      721 tgagggtgtt ggccctggaa ccgtccttgt tcagatagcc gaaccagtcg ccatgctgaa
      781 cgtcctggaa gtgggagaaa gcccagttgc gcaccatgtc gtggcggatg gcatagcgtt
      841 cttcccctgt gagcttgtag gcgagcgtca tggcgatgag cgcttcatca tgcggccacc
      901 agaacttcat gttgtgccag tattcctgaa cgggtttgtt gtacacgtcc gtgtagtaca
      961 gcatgccgcc gttttccttg tcccagccgc gggcgaatgc ccagtcaatc atgtcgcagc
     1021 ctaccttgat gagttcggga tcattaccac gatggcgggc ttcttccaga acgaaccatc
     1081 ctccctccgt agtgtggccg gggttcaggg tgcgttcgtc gaagtggtcg atgatggagc
     1141 cgtccgttcc caccacttcc atgactgcct ggatgtcagg tttcatgaat agggtgcgga
     1201 ggtcgttgat gcagcggtct gtccagccgg tatagaagcc gtcgtcatct cccaggtact
     1261 tgcgcatttc ctgagctgtg ttcagggtaa tcatgcgggt gcccagaccg gtcgtaggac
     1321 gttccccggt gaatttggga accattttgc cgggagtgaa gcagttgtcc gtgaagatgt
     1381 cgaaccagtg gcgggcttca cgggcagaat cccggctgcc ggtcgccctg gcgtgcgctg
     1441 caaaggcgat ggcggcgaaa gattcgctgt aggcgtagcg gcgtttgcgg atgggggtgc
     1501 cgtcggcggc tacatgaaag aacatgcggc cgtccgccgg gtcaacgcac ttggttttca
     1561 ggaattccaa ggcgctttcc gcccatttga gccagtccgt attcttttcg atgctgttgt
     1621 acattgtcag cagcatccat gccatgcggc cctgggccca gacggatttg tcggagtcca
     1681 ccagcgtgcc gtctgcgtca aagcagtggt aaagaccacc gtttttttcg tcgtaggcgc
     1741 gggggaacca gaacggcaga acgtcttcca gaagctggcg gcggtagaac ttgcccaaag
     1801 agggcatatc aagagttgca gccatgtcag tcaggttccg gggttcagtt taaagcccac
     1861 tggaagaagc cgatgccttc cagttcggaa cggagttcct tcagctgatc tgcggtcggg
     1921 ttgccctgcg gaagccgggc ggggccaaga tcgacgccca gccagcccat cagagccttg
     1981 gcgcagccca tatagccctt ggaggccagg atattgatca tctgaacgga tttccactgg
     2041 cagtcgcgcg ccgtttccac atcgccttcc gcaaaggctt tcatgagttt ctggtacagg
     2101 gccggagcaa agttgaagga gctccccaca gcgcccttgg cccccacgga aagggcgccc
     2161 gtaaaccatt cgtccacacc ccagggaata tccacggttt cgtcgtaatt cagcgtggtc
     2221 tggtacaggg ccagatccgg attggtgaat ttgatgcctg cgaaatttgg aatctgttcc
     2281 ttggccagcc tgatgaaatc caccggattg aagcgtacgc ccgtcagcac ggggatatcg
     2341 tagtaataat agggcaggtc gggagcgccg gaggcggcga aggcgcagca ttccaccagg
     2401 cactgaacag tgccgggctt gtagtaggac ggggccaggg aactggtggc cacgaatccg
     2461 cattcctggg caaaagaggc cagttcccgg gcgtcccaga cgctgttgga accggtatgg
     2521 gcgataactt ccacgccatg cttggcggag gcttccttcc aagcggaata gatttccttg
     2581 cgttcttcaa gctgcatgga ggaggattcc ccggtgctgc cggtaatgaa agcaagcttg
     2641 atgccctggg aggcaagcag cttggcctgg gcgtcaacgc tggaaggatt cagggaaccg
     2701 tccgccttga acggagtgtg tacggcggcc accaggccgt ggagtttcaa tgacgtgtcc
     2761 atattatgta gtgatatgaa aataattcgg ggcgatgatt g
//
LOCUS       DAOMSU010000280         2799 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3705_length_2799_cov_2.837828, whole genome shotgun sequence.
ACCESSION   DAOMSU010000280 DAOMSU010000000
VERSION     DAOMSU010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3705_length_2799_cov_2.837828"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..610)
                     /locus_tag="PK077_09545"
     CDS             complement(<1..610)
                     /locus_tag="PK077_09545"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HRN24580.1"
                     /translation="MEPIYEGKAKRLYTTDNPNVLRMEYKDDATAGNGAKKAQFENKG
                     RMNKAITLVIYRMLESKGVKTHLVADVDDINIDVKKVSILPLEVIVRNIAAGSFSRRM
                     GVKEGTPFKKPIVEFSYKDDSLGDPFINDDYAREMNAATEEECAFLKNQARIVNDVLI
                     DFFKQAGLTLVDFKIEFGRLAEDPSQIVLADEISPDTCRLWDI"
     gene            854..2701
                     /locus_tag="PK077_09550"
     CDS             854..2701
                     /locus_tag="PK077_09550"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HRN24581.1"
                     /translation="MHADLEKLLSLGKITPSLAEKLDRIAPGRYCFHASWGAGKVISW
                     NLPAKKLVIDFEENPEHEVALEFAPRILEFISDDHFLAKRYEDTESLINLSVDDPVEL
                     VRVTLQGYGNSLTPEKLEAALKGTVIAADKWKNWWDKVRAMLRSNVQFMMPTRKGERI
                     TLRANILSRAQAALEDYNKAADLKAKVRVLDGIKMEAVMAEPDAVNALIRAVDADVRN
                     GGSLALQQVLELAVLRDDLIASLKNTEAAKEAYPLRSIVEANIGDVGRFAEVLNSMPA
                     VRQKRVYATLPAIFGEDWPQKALELFDAGGARAVGEIAKFLIEEGQDKVLVKHLKHEL
                     LRQTLPAESLIWICRQRHDASKPLFGLPVGIAMLSLIEQDHMDGGPNRMLRLKNLFME
                     DKSIIQEMIKGQDVAEVRQFAKMLYNTSAFSEQDRGALMARIISVFPDLPAIVLDALV
                     DNSDKPEPIFVSWESLEARKKELEELVNVKIPENLHNKKISRAEGDLRENGGYQDAKE
                     VEKVLNRRRAELEHALALARGTDFAVTDTSRAAMGTKVILQPLNGGEPVVYTILGAWD
                     TNPEKHIVSYLSQVGKELTGKSVGDQVKIVPMDGDKKKVYTITKIEAAF"
BASE COUNT          618 a          744 c          834 g          603 t
ORIGIN      
        1 tgatgtccca caggcggcag gtatccggag aaatttcgtc tgccagcacg atttgggagg
       61 gatcttccgc caagcggccg aactcaattt tgaagtccac cagagtaagt ccggcctgtt
      121 tgaaaaagtc aatcagcaca tcattcacga tgcgggcctg atttttcagg aaggcgcatt
      181 cctcttccgt agcggcgttc atttcacggg cgtagtcgtc attaatgaag gggtcgccca
      241 ggctgtcatc cttgtaggag aattccacaa tcggcttctt gaaaggggtt ccttccttga
      301 cgcccatgcg gcggctgaag gagccggcgg cgatgttgcg cacgatcact tccagaggaa
      361 ggatggaaac cttttttacg tcaatgttga tgtcgtccac gtccgcaacc aggtgggttt
      421 tcacgccctt gctttccagc atgcggtaga tgaccagggt gatggccttg ttcatcctac
      481 ccttgttttc aaactgggcc ttcttggctc cgtttccggc agtggcgtcg tccttgtatt
      541 ccatgcggag gacgttggga ttgtcagtcg tataaaggcg tttggccttc ccttcgtaaa
      601 taggttccat gatacgtgtt gcgcaggggt tgaatgcgtg cggagatgtt acgccagacg
      661 ctccgcacgg tcaagccgca actggatgaa ggaggggagg gaaaaagaag cgaaacccga
      721 atgcgaagtc ggagcatgac gcagacggcc gcagtctccg gggcaacctt gaaaagccgc
      781 acggagggag tccggacgga agagggaaat tagtcttgat accggtaggg gggggcgtat
      841 catgtgcttt agtatgcacg ccgacttgga gaaattattg tctctgggga aaattacccc
      901 ctctctcgcc gaaaaacttg atcgcatcgc acctggccgt tactgcttcc atgcttcctg
      961 gggcgccgga aaagtcattt cctggaatct ccccgccaaa aagctggtca ttgattttga
     1021 ggaaaatccg gaacacgaag ttgccctgga attcgctccc agaattttgg agttcatcag
     1081 tgacgaccat ttcctggcca agcggtatga agataccgaa tccctgatca acctgtccgt
     1141 ggatgacccg gtggaactgg tgcgcgttac cttgcagggc tacggtaatt cccttactcc
     1201 ggagaagctg gaagctgcgc tgaaaggcac cgtaattgcg gcggacaagt ggaaaaactg
     1261 gtgggacaag gtgcgcgcca tgctccgttc caatgtccag ttcatgatgc ccacccgcaa
     1321 gggcgaacgc atcacgcttc gcgcgaatat cctttcccgc gcgcaggcgg ctctggaaga
     1381 ttacaataag gccgccgacc tgaaagccaa ggtgcgcgtg ctggacggca tcaaaatgga
     1441 agcggtcatg gcagagccgg atgccgtcaa tgccctgatt cgtgccgtgg atgctgatgt
     1501 gcgcaacggc ggcagcctgg ctctccagca ggtgctggag ctggccgtgc tcagggatga
     1561 cctgattgcc tcccttaaaa atacggaggc ggccaaggaa gcttatcccc ttcgttccat
     1621 cgtggaagcc aatatcggcg atgtgggccg cttcgcggaa gtgctcaatt ccatgcctgc
     1681 cgtgcggcag aagcgcgtgt atgccacgct gcccgccatc tttggggagg attggccgca
     1741 gaaggcgctg gagctttttg atgcaggcgg agcccgcgcc gtgggggaaa tcgccaaatt
     1801 cctgatagaa gaagggcagg acaaggtgct ggtcaagcat ttgaagcatg aattgctgcg
     1861 ccagaccctt ccggcggagt ccctcatctg gatatgccgc cagcggcatg acgcttccaa
     1921 gcctcttttt ggcctccctg tgggcattgc gatgctttcc ctgattgaac aggaccacat
     1981 ggacggcggc cccaaccgca tgctgcgcct gaagaacctg ttcatggagg ataagagcat
     2041 catccaggaa atgatcaagg ggcaggatgt ggcggaagtc cgccagtttg ccaagatgct
     2101 gtacaacacc tccgctttct ccgaacagga ccgcggcgcg ctgatggccc gcatcatcag
     2161 cgtattccca gacctgcccg ccattgtgct ggacgccctg gtggacaact ccgacaaacc
     2221 ggaaccgatt ttcgtttcct gggaaagcct ggaggcccgc aagaaggagc tggaagaact
     2281 ggtaaacgtg aaaattccgg aaaacctgca taacaagaag atttcccgtg cggaggggga
     2341 tctccgcgaa aacggcggct accaggatgc caaggaagtg gagaaggtgc tcaaccgccg
     2401 ccgggcggaa ctggaacatg cgctggccct ggcccgcggc acggactttg ccgtgacgga
     2461 tacctcccgc gccgccatgg gaacgaaggt gattcttcag ccgttgaatg gcggggagcc
     2521 cgtggtgtac accattctgg gcgcgtggga cacaaatccg gaaaagcata tcgtctccta
     2581 tctttcccag gtcggcaagg agctgacggg caaaagtgta ggggaccagg tgaagattgt
     2641 cccgatggac ggcgacaaga aaaaggtgta taccattacg aagattgaag ccgctttcta
     2701 aaggcgccgc ttcccttttt gccaaggtct ttaaagacgc cccttttttc ggaaaggggc
     2761 gtttttcatt gtgtatgggc cggcccctcg ttattttgc
//
LOCUS       DAOMSU010000281         2786 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3737_length_2786_cov_2.648847, whole genome shotgun sequence.
ACCESSION   DAOMSU010000281 DAOMSU010000000
VERSION     DAOMSU010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3737_length_2786_cov_2.648847"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PK077_09555"
     CDS             complement(<1..249)
                     /locus_tag="PK077_09555"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HRN24582.1"
                     /translation="MDTIDSITIIAAAWGLLVLRLTLAFILWPHGTQKVLGWFGGAGW
                     EGTYRAFTEKMGIPPFLAKVAMLTESFAPICLVLGVFTR"
     gene            complement(375..1553)
                     /locus_tag="PK077_09560"
     CDS             complement(375..1553)
                     /locus_tag="PK077_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="terpene cyclase/mutase family protein"
                     /protein_id="HRN24583.1"
                     /translation="MFKSVLSLTLAAALGTASFGQTTLTAIPGEAAAAKYASISQEIL
                     RAIEKGNEYLKSKQNPEGYWAQPSYPALTALAVTAYMRDPANQGKPVPEYIRKGYDFI
                     LKSQKEDGSIFNRGMSSYNTAVCMMALLAANKEEYAPAILKGRAYLIKQQNHFAPDNP
                     YNGGIGYGDKQAPPIADLSNTSLALEAIYYSQKLAKDGKYGEQPDLDWNAATEFINRC
                     QQNPAVNKEPWVSNDKSQLGGFVYRPGVSSARDKKSAAFDKAEPPKAYGSMTYAGMQS
                     LIYANVDKNDPRVKLALKWLENSYSLDENPGMGVQGLYYYYQAMSKALSAMGIDTLTL
                     ENGRKVDWRDELANKLISIQKSDGSWVNTNNRWWEADPVLVTSYCMLALEQLYHSIPR
                     "
     gene            complement(1560..2345)
                     /locus_tag="PK077_09565"
     CDS             complement(1560..2345)
                     /locus_tag="PK077_09565"
                     /inference="COORDINATES: protein motif:HMM:NF040466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdjY domain-containing protein"
                     /protein_id="HRN24584.1"
                     /translation="MFLKFTIALLACTWGFSCAQDISGPEEDTNAPAAEEATREVPLP
                     DGKTVPKFEPLDEHRIKIGHVIVNHKERTVSFQAQVNMKEGILEYVCCMPNGKLHESL
                     LVTEADPLHISLGMTLLKFHRFEKFFPVRDENFEWLPFTEPKPEDYADSYVQIVMTYT
                     ENGREQKSDFSDIVVNSQTRKGLNPSDWLYTNSFFYEGAYQASLSGEVISIFASRTSP
                     INYIGDFHDGVNDTGWIVNPKKNLSLGTNVTITISQKPVQPRQ"
     gene            complement(2460..>2786)
                     /locus_tag="PK077_09570"
     CDS             complement(2460..>2786)
                     /locus_tag="PK077_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRN24585.1"
                     /translation="TASVNDCLKAMTTAHAGACVLLTEDRKLAGIFTHGDFVRAYGAN
                     PLIGEQPVSGFMTRNPIYVMEEDLAAEAAKAVSNRRIDDLVVLNAEMTPVGIIDLQDL
                     ARLKLV"
BASE COUNT          540 a          705 c          867 g          674 t
ORIGIN      
        1 tctggtgaat acgcccagta ccaggcagat aggggcgaaa gattccgtca gcatggccac
       61 cttggccaga aaggggggaa tgcccatttt ttccgtgaag gcccggtagg tgccctccca
      121 gcccgcgccc ccgaaccagc ccagcacttt ctgggtccca tgtggccaca ggataaaagc
      181 cagcgtcagc cggagaacca gcaggcccca tgccgctgca ataatggtaa tggaatcaat
      241 ggtgtccatg aatttgaagt gattgaagaa aagacccttc tatttcatgg ggcgttcatt
      301 tccgggtacg gaaaaggccg gaaaaggagg tcattgcctc cccttccggc ccgggaaatc
      361 agcatggcag tccgttaccg gggaatggag tggtagagct gttccagcgc cagcatgcag
      421 taggaggtca ccaggacggg gtccgcctcc caccagcggt tgttggtgtt cacccaggaa
      481 ccgtcgctct tctggatgga aatgagcttg ttggccagtt catcccgcca gtccaccttg
      541 cggccgtttt ccagcgtcag ggtatctatt cccatggcgc tgagcgcctt ggacatagcc
      601 tgatagtagt agtaaagccc ctggacgccc atgccggggt tttcgtccag gctgtagctg
      661 ttttccagcc acttgagggc cagcttgacg cgggggtcgt ttttatccac attggcgtaa
      721 ataagggact gcatgcccgc atacgtcatg ctgccgtaag ctttgggggg ctccgctttg
      781 tcaaaggcgg cgcttttttt gtccctggcg ctggagacgc cgggacggta cacgaagccg
      841 ccgagctggg atttgtcatt ggaaacccag ggttccttgt ttacggcagg gttttgctgg
      901 caacggttga tgaattccgt ggcggcgttc cagtccagat ccggctgttc accgtacttg
      961 ccgtctttgg cgagtttctg ggagtagtaa atggcttcca gcgccaggga ggtattggag
     1021 aggtctgcaa tgggaggagc ctgcttgtcg ccgtagccga tgccgccgtt ataagggttg
     1081 tccggggcaa agtggttctg ctgcttgatg aggtaggcgc gacccttgag aatggcgggg
     1141 gcatattcct ccttgttggc ggccagcagg gccatcatgc acacggcagt attgtaggag
     1201 gacatgccgc ggttgaaaat ggagccgtct tccttctggg atttcaggat gaagtcatac
     1261 cccttccgga tgtattccgg gacgggtttc ccctggttgg cggggtcccg catgtaggcg
     1321 gtgaccgcca gggctgtcag ggcagggtag gacggctggg cccagtaacc ttccggattt
     1381 tgtttgcttt tcaggtattc gttccctttt tcaatggcac gcagaatttc ctggctaatg
     1441 gaggcgtatt tggcggctgc cgcttctccg ggaatggccg tcagggtggt ctgcccgaag
     1501 gaggctgtgc ccagtgcggc ggccagcgtt agtgaaagta cggatttgaa cataaggtgt
     1561 tattgcctgg gttgaaccgg tttctgggaa atggtgatgg taacgttcgt gcccagagac
     1621 aggtttttct tcgggttgac gatccagccc gtgtcgttga cgccgtcatg gaagtctccg
     1681 atgtagttga tgggggaggt acggctcgcg aaaatggaga ttacttcccc gctcaggctg
     1741 gcctggtagg cgccttcgta aaagaaggaa ttggtgtaaa gccagtcgct gggattcagt
     1801 cccttccgcg tttgtgagtt cacgacgatg tcggaaaaat cgctcttctg ttccctgccg
     1861 ttttccgtat aggtcatgac aatctgcacg taggagtctg cgtagtcctc cggcttgggt
     1921 tccgtgaagg gcagccattc aaaattttca tccctcaccg ggaaaaattt ttcaaagcgg
     1981 tggaatttga gcagcgtcat tcccaggctg atgtgcaggg gatccgcctc cgtcaccagc
     2041 agggattcat gcagcttgcc gttgggcatg cagcagacgt attccagaat tccttccttc
     2101 atgttgactt gagcttgaaa actgacggta cgctccttgt ggttgacaat cacatggcct
     2161 attttgatgc ggtgctcatc caggggttca aatttgggaa ccgttttccc gtccggaaga
     2221 gggacttccc gcgtggcttc ctccgcagcg ggggcattgg tatcttcctc cgggccggag
     2281 atgtcctgtg cgcaggaaaa gccccaggtg caggcaagca gggcgatggt aaatttcagg
     2341 aacatgttta tggaattaag attgtggaaa cggaagcttc cgttcaatga aaaaggcatc
     2401 cgtgactgat ttgttacttt tgaatcacgg atgcctggaa agaatgggaa agggagggtt
     2461 taaaccagtt tcagcctggc gaggtcctgg aggtcaatga tgcccacggg agtcatttcc
     2521 gcattgagca ccaccaggtc gtcaatgcgg cggttgctca cggccttcgc cgcttcagcg
     2581 gccaggtctt cttccatgac gtaaatggga ttgcgggtca tgaatccgct gacgggctgt
     2641 tctccgatga gagggttcgc cccgtatgcc cggacaaagt ctccgtgggt gaagatgccg
     2701 gccagcttgc ggtcctccgt cagcaggacg caggcgccgg cgtgggccgt ggtcatcgcc
     2761 ttcaggcagt cgttcacgga ggcggt
//
LOCUS       DAOMSU010000282         2767 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3769_length_2767_cov_2.520280, whole genome shotgun sequence.
ACCESSION   DAOMSU010000282 DAOMSU010000000
VERSION     DAOMSU010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3769_length_2767_cov_2.520280"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /gene="tsaB"
                     /locus_tag="PK077_09575"
     CDS             complement(<1..458)
                     /gene="tsaB"
                     /locus_tag="PK077_09575"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRN24586.1"
                     /translation="MQDVLLVLETSCVQSSVAAWSGESLLCQAAWRAERNHSSAIFKA
                     VRQVLDALEGRRLKEIVVGSGPGAYSGIRVALAVADGLSLVHGSRVAAFSPWNGLGLH
                     HGEACVMSDARRGGWTWGRLENGVLTDVPAVLPAEQARACVAECMEKGVPV"
     gene            679..1491
                     /locus_tag="PK077_09580"
     CDS             679..1491
                     /locus_tag="PK077_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24587.1"
                     /translation="MMRRCNRDAWNKTYRAQVSSEYWEDECQEFSFMDLPRRTRFIRH
                     AVATLILLPLALITAMSLCDQLTHASGSLNILFSIPVWYALMGAAVWVVLGSSRLFTS
                     SLLYLYVLGHELTHALAVICSRGSIHAFKADLDGGYIQTDKNNIFISLSPYFLPLWAM
                     LWGLVWGVTYLFWPTVTCQAILYSGLGFWWCFHLFWTAWIIPKDQPDLADNGTFFSLI
                     LVYFANLLLFLALLRLFNAVSLHRFLADCIHNAEKLWDVLRDLAMLAAGLFL"
     gene            1572..2093
                     /locus_tag="PK077_09585"
     CDS             1572..2093
                     /locus_tag="PK077_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24588.1"
                     /translation="MRTTHLIILTMAAVSLMGCNPKETVNALTSDQNVTQKMAVRFAE
                     NMVGLPRALVVSDARKAAPGDKVTVKGAVIGDDPVFSKNIASFTIGDPAQLLVNMKEK
                     GVWAAGTVPVEIRKNNTMTVQFVDEQGQPIKHSAKGVNGLKEQDAVIITGTIDPHSTP
                     ERPVLNIESIVIE"
     gene            2205..2657
                     /locus_tag="PK077_09590"
     CDS             2205..2657
                     /locus_tag="PK077_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24589.1"
                     /translation="MITLAATSAAAGAASASAQKKSAEATRQAADANAATLRNEADLQ
                     KQTGMENAKRMQQDAARQMGEARLDAAQSSLLREGSVPIREQALATRLQTRIQDEAAE
                     AMRNAQSLYNRANTEQYMGRSNARAQKTSAMGTLLSGFAQGGNTLLKW"
BASE COUNT          642 a          880 c          710 g          535 t
ORIGIN      
        1 acgggcacgc ctttttccat gcactccgcc acgcaggcgc gggcctgctc cgcaggcagc
       61 actgccggca catccgtcag aacgccgttt tccagcctcc cccacgtcca gccgccacgc
      121 cgggcatctg acattacgca ggcctcacca tgatgcagac caagtccgtt ccagggggaa
      181 aaagccgcca cgcgggaacc gtgcaccagg gaaagtccgt ccgccacggc cagcgctacg
      241 cggattccgc tgtaagcgcc cggaccggaa cctaccacga tttccttcaa acgccgccct
      301 tccaaagcat ccagaacctg ccgcaccgcc ttgaaaatag cggaggaatg gttgcgctcc
      361 gctctccatg ccgcctggca cagcagactt tccccgctcc aagccgcaac ggaagactgc
      421 acacaggaag tttccagaac cagcaggaca tcttgcatgc ggcggattat acaggaacag
      481 ccggcgtgga taaaacattt tttcctccgg tttccggaag aggcaaattt ggttcgcatt
      541 tctcctcaag tccctttatg ctggttccag acctcacacc gcatgcccaa ggcccccaga
      601 cacacaccgc cgcgccagga ctggcgccgg tatgcagaaa ccgcccggaa accaagatcc
      661 gcggcaagcc tcggcgacat gatgcggcga tgcaaccggg acgcctggaa caagacttac
      721 cgcgcccagg tatcctccga atactgggag gacgaatgcc aggaattttc cttcatggat
      781 ctcccgcgca gaacccggtt catcaggcat gccgtagcca cccttatcct gcttccgctg
      841 gccctgatca cggccatgtc cctgtgcgac cagctgaccc atgcctccgg cagcctcaat
      901 atcctcttct ccattcccgt atggtacgcc ctgatgggcg ctgccgtgtg ggtggtgctg
      961 ggcagcagca ggctgttcac atcctccctg ctgtacctgt acgtattggg gcatgaactg
     1021 acgcacgctc tggcggtcat ttgcagccgc ggctccatcc atgctttcaa agcggatctg
     1081 gacggagggt acatccagac ggacaaaaac aacatcttta tctccctttc cccctacttc
     1141 cttcctctct gggccatgct gtggggattg gtatggggcg taacgtacct cttctggcct
     1201 acagtcacct gccaggccat tctttactcc ggcctgggct tctggtggtg cttccacctg
     1261 ttctggacgg catggatcat tcccaaggac cagccggacc tggcggacaa cggcaccttc
     1321 ttctccctga ttctcgtata ttttgccaat cttcttctct tcctggcgct cctgcgcctg
     1381 ttcaatgccg taagccttca ccgctttctg gcggattgca tccacaatgc ggaaaaattg
     1441 tgggacgttc tcagggatct cgccatgctg gctgccgggc tgttcctgta aaaaatattc
     1501 ttttccttac acggaatgat gaacggcaga aggggaaaag gaatccaacc tttcaataga
     1561 aaggagtttc catgcgcacc acacacttga tcattctgac aatggccgca gtttccctga
     1621 tgggctgcaa tcccaaggaa actgtaaacg ccctcacttc cgaccagaac gttacccaga
     1681 agatggccgt aagattcgcg gaaaatatgg ttggcctgcc acgcgctctg gtcgtttcag
     1741 atgccagaaa agcggctccg ggcgacaaag tcaccgtcaa gggagcggtg atcggagatg
     1801 accccgtctt ttcaaaaaac attgcttcct tcaccatcgg agacccagct cagctcctgg
     1861 tcaatatgaa ggagaaggga gtctgggccg ccggtacggt accggtggaa atacggaaaa
     1921 acaatacgat gaccgtccag ttcgtggatg aacaggggca gcccatcaag cactccgcca
     1981 aaggcgtgaa cgggctgaaa gaacaagacg ccgtcatcat taccggaacc attgatcccc
     2041 attccactcc ggaacgtccg gtgctgaaca ttgaaagcat cgtgattgag taacccctct
     2101 tctgtgccaa accctgattc atgtcaaacg gttcaggcgt tttgcgcctg aaccgttttt
     2161 catatcagaa tgcgcgcatg ggatttgatc cagcatcatt gggaatgata acactggccg
     2221 ctacttcagc ggcagcaggt gcggcatccg cgtctgcaca gaaaaaaagc gcggaagcca
     2281 ccagacaggc ggcggacgca aatgccgcca ccctcaggaa cgaggcagac ctccaaaaac
     2341 agacagggat ggaaaatgcc aaacgcatgc agcaggacgc tgcgcgccaa atgggagaag
     2401 cccgactgga cgccgcccaa tcctccctgc tccgggaagg ctccgttcca atcagggaac
     2461 aggctttggc gacgcgtctg caaaccagga ttcaggatga agcggcggaa gccatgagga
     2521 acgcccaatc cctgtacaac cgcgccaaca cggagcaata catgggccgc tccaatgcga
     2581 gagcccagaa aacctcagca atgggcaccc tgctctccgg attcgcccag ggaggaaata
     2641 ccctcctcaa atggtgaagt gcctcacaag gcacctccat tcaaacgaag acagccggaa
     2701 attcagccgc aaccgcggaa gcttcttaac gccccccctg ccgggctgcc ccggaagagc
     2761 aacctcc
//
LOCUS       DAOMSU010000283         2740 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3816_length_2740_cov_2.002980, whole genome shotgun sequence.
ACCESSION   DAOMSU010000283 DAOMSU010000000
VERSION     DAOMSU010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3816_length_2740_cov_2.002980"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..610)
                     /locus_tag="PK077_09595"
     CDS             complement(14..610)
                     /locus_tag="PK077_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRN24590.1"
                     /translation="MNSANTPLLETERLILRKFTENDLEALFNIYSNEEVNTYLPWFP
                     LTSLEEAASLFKEKYQEVYKRSYGYHYAICPKNSNLPAGYIHAGTDDSHDLGYGLLQE
                     FWHRGFATEAARAVVARLKKDGMPYITATHDIRNPRSGGVMKQLGMRYQYSYEELWQP
                     KNIPVTFRLYQLNLDGQEDRVYQEYWNRSSVRFKETDI"
     gene            913..1488
                     /locus_tag="PK077_09600"
     CDS             913..1488
                     /locus_tag="PK077_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRN24591.1"
                     /translation="MKSIKGTETEKNLLKAFAGESEARNRYTYFAGVAKKAGYEQIAA
                     IFLETADNEKEHAKVFFKLLKDACVEVTHAVCTAPLESTEDCLLAAAAGEHDEWSDMY
                     PAFAEVADREGFPAIAETFRKIASVEQHHEARYLKLAENVREGRVFAKEKEIQWKCRN
                     CGYVHTGSTAPKVCPACAHPQAFFEELADNF"
     gene            1537..>1733
                     /locus_tag="PK077_09605"
     CDS             1537..>1733
                     /locus_tag="PK077_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24592.1"
                     /translation="MQSALFLDYGRLTLMDRERTDAVWKKYGRSLWNFAGSYGLGIAL
                     MLILLVLTFAGTLHQVRLSSA"
     assembly_gap    1734..1743
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1744..>2740
                     /locus_tag="PK077_09610"
     CDS             <1744..>2740
                     /locus_tag="PK077_09610"
                     /inference="COORDINATES: protein motif:HMM:NF016995.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HRN24593.1"
                     /translation="AEAAIESFFGAAYVLIPLGGENSLISLPLPGMGITCVLLFANLL
                     IGGVFRIRWTWRHAGVLVAHGGILLLLAGIMLGNKMTVAVEQVELPQGDRVHEYSLPF
                     DLRLNRFVPEFYPGTSKPKSYESQITVFPESGGQYDAVIRMNEPLRLSGWTLYQMSWG
                     QDSLHPGRLISILRASHNPLEQMPKWSSYIIAIGLLWHFACVFGRYLRRKPGLASAGT
                     AATDELQAVSAPGGKKRLRLAGICLLVAAIFGVGMLAARPDVHPVLVEHYVPWSPFLV
                     ERAGAMAVQDGGRLKPVSTYAGFHLLRTLGKRSFVVDTPEGKRKLSPVEWMLDCM"
BASE COUNT          552 a          701 c          771 g          706 t
ORIGIN      
        1 aggtattggg aattcagata tcagtttctt tgaaacggac ggatgacctg ttccagtatt
       61 cctgataaac ccgatcctcc tgtccatcca gattcaactg gtacaggcgg aaagtgaccg
      121 gaatattttt gggttgccac agctcttcat aggaatattg gtagcgcatg cccagctgtt
      181 tcatgacgcc tccgctgcgg ggattcctga tatcgtgtgt ggccgtgatg tagggcatgc
      241 cgtctttctt caaccgggcg acaacagccc tggccgcctc cgtggcaaag cctctgtgcc
      301 agaactcctg aagcaatccg tagccgaggt catggctgtc gtccgttcct gcgtggatat
      361 accctgccgg gaggttgctg tttttcgggc agatggcgta atgatacccg taagaccgtt
      421 tgtacacttc ctggtacttt tctttgaaca gggacgccgc ttcttccagg gaggttaatg
      481 gaaaccacgg caaatacgtg tttacctctt cattgctgta aatgttgaac agggcctcca
      541 gatcattttc cgtgaatttt ctcagtatta atctctcggt ttccaataag ggggtattgg
      601 cggaattcat ggatggagct taaggttttt ttccgtttaa ttttactatt tacacaaggg
      661 gtgtcaaatg atccggttca tgcttaggcc tgatgttgcg gtacgggttc ctgcctgttt
      721 cccgggatgc cgttttgctg ccggcgcgta tggggggaca gcagaagcgt gcatggcaac
      781 agttttccca aatggggaaa ctgccgtatg tttttccgga tatgtctatt ttttagattc
      841 attcaatatt tgcttgtttt ttcacggcgt cttattagga tgctttcgtt tacgcaacca
      901 gatgactaat ttatgaaatc catcaaagga acagaaacag aaaagaatct tctcaaggct
      961 tttgccgggg agtcagaagc gcgcaaccgc tacacctact ttgccggcgt agccaagaag
     1021 gccggttacg aacagattgc cgctatcttt ctggaaacgg ccgacaatga gaaggaacat
     1081 gccaaggtat ttttcaaact gctgaaagac gcttgcgttg aagtaacgca cgccgtctgc
     1141 acggctcccc tggaatctac ggaggattgt ctgctggccg cagccgccgg cgagcatgac
     1201 gaatggtccg acatgtatcc tgcgtttgcc gaagtagcgg acagggaagg attccccgcc
     1261 attgcggaaa ctttccgcaa gattgcctcc gtggagcagc accatgaagc ccgctacctc
     1321 aagctggctg aaaatgtgcg cgaaggcagg gtttttgcca aggagaagga aatccagtgg
     1381 aagtgccgca actgcggtta tgtacatacc ggttccactg cccccaaggt atgtccggcc
     1441 tgtgcacatc cccaggcatt ctttgaagaa ctggcggaca acttttaata accttttccc
     1501 caggaatatg attggggccg gtttcacgag ctgatcgtgc aatcggccct ctttttagac
     1561 tacggacgcc ttacgctgat ggacagggaa cgaacggatg cggtatggaa aaaatatgga
     1621 cgcagcctct ggaattttgc cggaagctac ggcttgggca ttgccctgat gctgatcctt
     1681 ctggtgctga cctttgccgg gacgctgcac caggtgcggt tgtcttctgc catnnnnnnn
     1741 nnngggcgga ggctgccata gagtcttttt tcggggcggc ttatgtgctg ataccgctgg
     1801 gcggggagaa ctccctgatt tccctgcctt tgcccggcat gggcattact tgtgtcctgc
     1861 tgttcgccaa cctgctcata ggcggggtat tccgtatccg gtggacttgg aggcacgcag
     1921 gcgtgctggt ggctcatggg ggaattttgc tgctgctggc cggcatcatg ctgggcaaca
     1981 aaatgacggt ggccgtggaa caggtggaac tgccccaggg ggaccgggtg catgaatatt
     2041 cccttccgtt tgatttgcgc ctgaaccgct ttgtgccgga gttttatccg gggacttcca
     2101 agcctaaatc ctatgaatcc cagataaccg tttttccgga atccggcggt cagtatgacg
     2161 ccgttatccg catgaacgag cctctgcgcc tttccggctg gacgctgtac cagatgagct
     2221 ggggccagga ttccctgcat ccgggcaggc tcatctccat tctccgtgct tcccataatc
     2281 ccctggagca aatgcccaaa tggtcctcct acatcatcgc tataggattg ctgtggcatt
     2341 ttgcctgtgt ttttggcaga tacctgcgcc gcaaacccgg tctggcatct gccggaacgg
     2401 cggcgacgga tgaactgcag gccgtttccg ccccaggcgg gaaaaaacgt ctgcgcctgg
     2461 ctggcatttg cctgctggta gcggccatct ttggcgtcgg catgctggcg gcgcggcctg
     2521 acgtccatcc ggttctggtg gaacattatg tgccctggtc tccctttttg gtggagcggg
     2581 ccggggccat ggccgtccag gacggcggcc gcctgaagcc tgtttctact tatgcgggct
     2641 tccatctgct ccggacactg ggcaaaagaa gctttgttgt tgatacgccg gaagggaaaa
     2701 ggaagctctc cccggtggaa tggatgctgg actgcatgtt
//
LOCUS       DAOMSU010000284         2721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3849_length_2721_cov_3.454989, whole genome shotgun sequence.
ACCESSION   DAOMSU010000284 DAOMSU010000000
VERSION     DAOMSU010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3849_length_2721_cov_3.454989"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1608
                     /locus_tag="PK077_09615"
     CDS             91..1608
                     /locus_tag="PK077_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HRN24594.1"
                     /translation="MNGQPVHEMAGPFSNMNGYCYFILGILALAVGYFTYGRVLERIF
                     RPDASRQTPAVACTDGVDYVVMPRWRVFLIQLLNIAGLGPIFGAVMGVLYGPAALLWI
                     VFGCILGGMAHDYFSGMISLRHKGENLPEILGRYLGNQAQWISRAVCIVFSVLVGVVF
                     AVGPAAILSPMTGWSVSAWVWIIFGYYFLATILPIQAIMGKVYPLFSVALIIMVMGIL
                     GVMLLAPFADSMPAWMHLPRMEVLPDLDFFHNRHPADFPLFPVMFITIACGAVSGFHA
                     TQSPLMARCLKTEREGLPVFGGAMITEGIIAFIWAAAALTFYGSPEALGAATANGKAP
                     ALAIQTISESWMGSVGSILVMIGVVILPISTGDGALRVTRLMIADSFKLSQEQLSRRL
                     MIAIPLFAAAIAVSSMDYAVIWQYFGWANQLLAAATLWAVSIYLRSKNRCCWPAAAPA
                     AFLSLVVFQYLFSSSEMCGFSEEASLIASVLLTAVIGVLCLFRGSGLEKAEEPSL"
     gene            1613..2500
                     /locus_tag="PK077_09620"
     CDS             1613..2500
                     /locus_tag="PK077_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24595.1"
                     /translation="MNSSAHTPGHARKVPWGAFWRLVLIQAQNSFNEKGAQFLLIPLG
                     VWLYGAEGDLEYPLGAIIVLPFILFSPFVGWLSDRFCKARIIQFMALLQICVLAGMYW
                     SLRVHNLNGAILWFCVFAVQATVFSPAKKGIVKDIVGTSRMGFASGIVEMASILSLLI
                     GQIGVFIWFRYLLEPSTDTIWHRWLGEGFFQWFDACLKPSGINDGWYAASFPCLVFLL
                     LAVPVAASSFRLPRYAPGGGPPLLLVPVLRALPSAGQAVEKPQSARQRDGHRLFLVLR
                     RHGHADDYPDGQGNLRWRG"
     gene            2451..>2721
                     /locus_tag="PK077_09625"
     CDS             2451..>2721
                     /locus_tag="PK077_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24596.1"
                     /translation="MLMTIQMAKEISGGGDDFSSVGAVLMAWMSGGTVLGGILASVIC
                     RRHIRMNVSVAGGVLMAASCAALAAVSMTSPVFNALLMAAGISAAL"
BASE COUNT          426 a          840 c          813 g          642 t
ORIGIN      
        1 gtttagtgtt gacttcggat ttgtaatccg ttccctattc ccctcctttc actaacagct
       61 tgccagaagg cgggagaaaa gttaagttcc gtgaacggac agcctgtcca tgagatggct
      121 ggcccttttt ccaacatgaa cggttactgc tatttcattc tggggatatt ggccctggcg
      181 gtcggttatt tcacttacgg acgggtgctg gaaaggattt tccggccgga cgcctcccgg
      241 caaactcccg ccgtggcctg tacggacggc gtggattatg tggtcatgcc gcgctggcgc
      301 gtttttctca tccagctgct gaacattgca gggctggggc ctattttcgg cgccgtcatg
      361 ggcgtgctgt atggcccggc cgcactcctg tggatcgttt tcggctgcat tctgggcgga
      421 atggcgcacg actacttttc cggcatgatt tccctgcggc acaaagggga aaacctgccg
      481 gaaatcctgg ggcgctacct gggcaaccag gcccagtgga tcagccgcgc cgtctgcatc
      541 gtgttcagcg tgctggtggg cgtcgtcttc gccgtggggc ccgccgccat cctgtccccc
      601 atgacgggct ggagcgtttc ggcatgggtt tggattatct tcggctatta ctttctggcg
      661 accattcttc ccatccaggc tatcatggga aaggtctatc ctcttttttc ggtcgccctg
      721 atcatcatgg tcatgggcat tctgggcgtg atgctcctgg ctccctttgc ggattccatg
      781 ccggcctgga tgcacctgcc ccgcatggaa gtgcttccgg atctggactt tttccataac
      841 cgccatccag cggatttccc gcttttcccc gtgatgttca tcaccatcgc ctgcggagcc
      901 gtgagcggat tccatgccac gcagtccccg ctgatggcgc ggtgcctgaa aacggagcgg
      961 gaagggctgc ccgtctttgg cggggcgatg attacggaag gcatcatcgc cttcatatgg
     1021 gccgctgccg cgctgacctt ctacggctct ccggaagccc tgggagcggc taccgccaac
     1081 ggaaaggctc cggcgctggc catccagacg atttccgaat cctggatggg aagcgtcggt
     1141 tccatcctgg tcatgatcgg cgtggtcatc ctccccatca gcacggggga cggcgccctg
     1201 cgggtcacac gcctgatgat tgcggatagt ttcaagctga gccaggaaca gctgagccgc
     1261 cgcctgatga tcgccatccc tctcttcgcc gcggccattg ccgtcagcag catggactac
     1321 gccgtcatct ggcagtactt cggctgggcc aaccagctgc tggccgccgc cacgctctgg
     1381 gccgtttcca tttacctgcg cagcaaaaac cgctgctgct ggccggcggc ggctcccgcc
     1441 gccttcctga gcctggtagt attccaatat ctgttctcca gctcggaaat gtgcggtttc
     1501 agtgaagaag catccctgat tgccagcgtc ctgctgaccg ccgtcatcgg cgtgctgtgc
     1561 ctgttccgcg gctccggcct ggaaaaagcg gaggagccca gcctgtaact tcatgaattc
     1621 cagcgcccat acgccaggac acgcccgcaa agttccatgg ggagctttct ggcggcttgt
     1681 gctgattcag gcgcagaact cctttaatga aaaaggggct caattcctgc tgattcccct
     1741 gggcgtctgg ctgtacggcg cagaggggga cctggaatat ccgctggggg ccatcatcgt
     1801 gctgcccttc atcctgttct ccccattcgt cggctggctt tcggaccgtt tctgcaaggc
     1861 gcgcatcatc caattcatgg ccctgctcca gatatgcgtg ctggcgggca tgtactggag
     1921 cctgcgcgtc cacaatctga acggggccat cctgtggttc tgcgtctttg ccgtgcaggc
     1981 caccgtcttc agcccggcca aaaagggaat cgtcaaggac atcgtgggga cctcccgcat
     2041 ggggttcgcc agcggcattg tggaaatggc ttccatcctc agcctgctca tcggccagat
     2101 cggcgtcttc atctggttcc gctacctgct ggaaccttcc acggatacca tctggcaccg
     2161 ctggctgggg gagggcttct tccagtggtt tgacgcatgt ctgaaaccct ccggcatcaa
     2221 tgacggatgg tatgccgcct ctttcccatg cctggtgttt ctgctcctgg ccgttccggt
     2281 ggcggcctcc agcttccgcc tgccccggta cgctccaggg gggggaccgc cccttctcct
     2341 ggtccctgtt ttacgagcac ttccatcagc tgggcaagct gtggaaaaac cgcaatctgc
     2401 gcgtcagcga gatgggcatc ggctatttct ggttcttcgg cggcacggtc atgctgatga
     2461 ctatccagat ggccaaggaa atctccggtg gcggggatga cttcagctcc gtgggagccg
     2521 tgctgatggc ctggatgagc gggggaacgg tgctgggggg catcctcgcc tccgtgattt
     2581 gccgccgcca catccggatg aatgtttccg tggcgggagg cgtgctgatg gcggcgagct
     2641 gcgcggccct ggccgcagtc tccatgactt ccccggtctt taatgccctg ctgatggcgg
     2701 cgggcatttc cgccgccctg t
//
LOCUS       DAOMSU010000285         2707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3872_length_2707_cov_2.917044, whole genome shotgun sequence.
ACCESSION   DAOMSU010000285 DAOMSU010000000
VERSION     DAOMSU010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3872_length_2707_cov_2.917044"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PK077_09630"
     CDS             complement(<1..383)
                     /locus_tag="PK077_09630"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HRN24597.1"
                     /translation="MKQSLAARFLFRAVVLAFLVYAMLLTWWTPFTGDSLMHSVFGAD
                     HRLAFQPVLERCWWSYMHWNPRLGEFLAIFTATAGKWLFLAVNPFVLLSLALMMFFLA
                     QGRRVNSGNWRDVLLFAAGALLLLTS"
     gene            complement(417..1862)
                     /gene="rseP"
                     /locus_tag="PK077_09635"
     CDS             complement(417..1862)
                     /gene="rseP"
                     /locus_tag="PK077_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRN24598.1"
                     /translation="MDSILSVLATIAIIFGVVMLFNFMIFIHELGHFFAARWRGLYVD
                     RFQIWFGRPIWKKTVNGVQWGLGWIPAGGFVSLPQMAPMEAIEGRAELPKDLKPVTPL
                     DKIIVAAAGPAASFLLAVLFAVAVWMVGKPDVEMGVTTVGFVAPDSPAAQAGILPGDK
                     IVKVDGHPVDKWAGNMEGVRELIMLGEHDRVVFTVQRPGHEGEMEISCGFRIPETSWW
                     QRSGMRQVGLMQAMPCVIGEVIPNSPAALAGLNPGDEVTAANGERLWNPAALDVLLKK
                     NEPLLLDVTDRAGVARQVNIQGKLPENWHNGADGSLLKGAQPILGVSWDLSSVGRDVT
                     VHPSPWAQIKQSLKWMGDTLAKVVAPGSSVGVEHLSGPVGIANQFYKMFSLDEGWKLA
                     LWFSVVLNVNLAVLNILPLPVVDGGHVVMNAVELIFRRPLNTKVLEFVQFGFVFLLMG
                     FFLFVTFKDVGDFFGKKPDKLPTPVFKAVAD"
     gene            complement(2481..>2707)
                     /gene="rplT"
                     /locus_tag="PK077_09640"
     CDS             complement(2481..>2707)
                     /gene="rplT"
                     /locus_tag="PK077_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRN24599.1"
                     /translation="KGQFRRLWIARISAAVRDRGLTYSRFMEGLKAANIDLDRKVLAD
                     LAVSDEKAFDAIFAQAKKAIEAKDGAALRA"
BASE COUNT          729 a          828 c          662 g          488 t
ORIGIN      
        1 gacgtaagca aaagaagcgc ccctgcggca aacagcagca catcccgcca gtttccggaa
       61 ttgacccttc ttccctgcgc caggaaaaac atcatcaacg ccagggaaag aagcacgaag
      121 ggattgacgg ccagaaaaag ccattttccc gccgtagctg taaaaatagc gaggaactct
      181 cccagccggg gattccagtg catgtatgac caccagcacc gctccagcac gggctgaaag
      241 gcaaggcggt ggtccgcccc gaacacggaa tgcatcaagc tgtccccggt gaaaggagtc
      301 caccaggtaa gcagcatggc atacacaaga aacgccagca ccaccgcccg gaacaggaaa
      361 cgcgccgcca gtgactgctt catggaacag gccgggataa aaaacgccgt ccgcaactaa
      421 tccgcgacgg ccttgaatac cggggtgggc agcttgtccg gcttcttgcc aaagaaatcc
      481 cccacatcct tgaatgtcac gaacaggaag aaccccatca gcaggaacac aaagccgaac
      541 tggacgaatt ccaggacttt tgtattcagg ggccgccgga aaatcaattc aacggcattc
      601 atgaccacat ggccgccatc caccaccgga agaggcagaa tattcagaac tgccaggttc
      661 acgttcaaca ccacggaaaa ccacagggcc agcttccacc cttcatccag agagaacatc
      721 ttataaaact gattggcaat gcccacaggt ccggaaagat gctccactcc cacgctgctg
      781 ccgggagcta ccaccttcgc cagggtatcc cccatccatt tcagactctg cttgatctgc
      841 gcccacggag aaggatggac ggtgacgtca cggcccacgg aactcaggtc ccagctcacg
      901 cccagaatag gctgggcccc cttgagcagg gaaccatccg caccattgtg ccaattctcc
      961 ggaagcttcc cctgaatatt cacctgcctt gcaaccccgg ccctgtccgt cacatccagc
     1021 aagagcggtt cattcttctt cagcagaaca tccagtgctg cagggttcca gaggcgttct
     1081 ccattggcgg ccgtaacctc atcgcccgga ttcaatccgg ccagggcggc aggagaattg
     1141 gggatcactt ccccaatcac gcagggcatg gcctgcatca accccacctg acgcatgccg
     1201 gagcgctgcc accaggacgt ttccggaatc cggaatccgc aggaaatttc catctctcct
     1261 tcatgtccag gtctctgcac cgtaaaaacc acccggtcat gctcgcccag catgatcaat
     1321 tcccgcacgc cttccatatt gcccgcccac ttgtccacag gatggccgtc cactttcaca
     1381 atcttgtcgc cgggaagaat gccggcctga gccgcagggc tgtccggagc aacaaaaccg
     1441 accgtagtca cccccatctc cacatccggc ttgcccacca tccagacagc cacggcaaac
     1501 aacacagcca gcagaaaaga agcggcaggc ccggcggcgg ccacgataat cttgtccaag
     1561 ggagtaaccg gcttcaaatc cttgggaagc tccgcacgtc cttcaatggc ctccatagga
     1621 gccatctgcg gcagggatac aaaacctccg gccggaatcc accccagccc ccactgcacg
     1681 ccgttaaccg tttttttcca gatgggacgg ccaaaccaga tttgaaacct gtccacatac
     1741 agaccacgcc agcgggcagc gaaaaaatga ccaagttcat gaataaaaat catgaagtta
     1801 aacaacatca ccacgccgaa aataatggcg atggtggcaa ggacggaaag aatgctatcc
     1861 atggtagttt ttgccgtcta acttaccacg ggcctctccg gagaacaagc ttcaaagatg
     1921 tcctaatgac aaagtggatg ctgatattcc aaatggattt caaaaggtgc ctgacagccc
     1981 agaaaacagc ctttaccttc cgggaattct gaagccgcaa gtaaaagacc gcgtcccctt
     2041 ttttcgagcg gtgcctctgg gagaggcggc atcatcccgc cccggaacct ggacgaataa
     2101 aagcgccttt tttaccagga gaaaaaccgg cggggcatca aacgccgcag cccgcttttc
     2161 tgtccgaatc cctgaacagc ctttgcaata aatgcctccg gaattggaag caatgattaa
     2221 agagccattg tgttcttaac aaaaatgcgc actggcggaa acaggatttt tcccagccgg
     2281 catgggaaaa tcgacgctgc aataagagaa actggctggc gcactaaatg atgaacgtgc
     2341 gaacacctgc tccataaggc atttacagga aaatgccaac ggccggaact gtcatatccg
     2401 gacatgaaaa cagcctccgg aaaattattt ccggaggctg tcggttaacg tttactgaac
     2461 agatggaaac tcccgcacat tcaggcgcgg agagcggcgc catccttggc ttcaatggcc
     2521 ttcttcgctt gagcgaagat agcgtcaaaa gccttttcgt cagagacggc caaatcggca
     2581 agaaccttgc ggtccaaatc gatattggcg gctttcaagc cttccataaa gcgggaatag
     2641 gtcagaccgc ggtcgcggac ggcagcggaa atacgggcga tccaaagacg gcggaattgt
     2701 cccttcc
//
LOCUS       DAOMSU010000286         2707 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3873_length_2707_cov_2.607843, whole genome shotgun sequence.
ACCESSION   DAOMSU010000286 DAOMSU010000000
VERSION     DAOMSU010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3873_length_2707_cov_2.607843"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PK077_09645"
     CDS             complement(<1..574)
                     /locus_tag="PK077_09645"
                     /inference="COORDINATES: protein motif:HMM:NF014851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24600.1"
                     /translation="MMKVTTILITAACLAGSFSLYADAVSDREEAASSTGAYSVAGAL
                     RLPENVTKRDVYGMNVGWKLFKGEKVPEEAASLDVDDSSWESVNLPNGIELLPEEASG
                     CSNYQGPVWYRKTFIPPSRLEGRRNTLYFEGIMGKSEIWVNGEKAAEHFGGYLPVIVN
                     LDKWLKPGQKNVIAVKADNSNDVSYPPGKSQ"
     gene            845..1876
                     /locus_tag="PK077_09650"
     CDS             845..1876
                     /locus_tag="PK077_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRN24601.1"
                     /translation="MSSFFQKIVKLFSYDIGIDLGTANTLVNVKDQGIVLREPSVVAV
                     KGDKVLAVGDEAKRMLGRTPGSVTAIRPLKDGVIADFYITEEMLRYFIKKATSRYYLI
                     KPRVLIAIPSGITEVERRAVKESAEAAGARRVHLIEEPMAAAIGVGLPVQEAAGNMIV
                     DIGGGTTEVALISLSGIVYSRSVRCAGDELDDAIISYMRRTYNLMVGERTAEDIKIRI
                     GSAYPLPQELSIEVKGRDLVAGLPKTVTIRSEEVREALTEQLNTIVDAVRTTLERCPP
                     ELAADLVDRGIVLAGGGALLRGLDQLLHEQTGLPIHIAEDPLSAVAEGTGKALQELDF
                     FTNVTDAED"
     gene            2061..>2707
                     /locus_tag="PK077_09655"
     CDS             2061..>2707
                     /locus_tag="PK077_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HRN24602.1"
                     /translation="MFTKMLKKIKRAFGFGSCDPMEGSTIIINAEKLERRVALLENGV
                     LEEYTIEREGEDNIVGGIFKGRVKNIEPGLKAMFVDIGQEKNAFLHFWDALPAALDSS
                     LEEIEREGRPKKQQRKITSKDIPDIYPIGSEIMVQVTKGPIGTKGPRVTTNVSLAGRY
                     LVLMPFTDQFGISRKIEDPKERLRLRQIMQKLNVPDGMGIIMRTVAQGQRYRHFVR"
BASE COUNT          617 a          817 c          709 g          564 t
ORIGIN      
        1 cctgagattt gccgggggga taggaaacgt cgttggaatt gtccgccttc acggcgatga
       61 cgttcttttg tcccggtttc agccacttgt ccagattgac aatcacggga agatatccgc
      121 cgaaatgttc cgcggccttc tccccattca cccagatctc gctctttccc atgatgccct
      181 caaaatacag ggtgttcctc ctcccttcca gccgggaggg aggaataaat gttttccggt
      241 accataccgg cccctggtag ttggaacagc cgctggcctc ttccggaaga agctcaatgc
      301 cgttggggag gttgacggac tcccaggagg aatcgtcaac atccaggctg gccgcttcct
      361 ccggcacctt ctctcccttg aacagcttcc agcccacatt catgccgtaa acgtcccttt
      421 tcgttacgtt ttccggcagg cgaagagccc ctgccacgga ataggctccc gtagatgaag
      481 cggcctcctc ccgatcggaa accgcatcgg cataaaggga aaaagagccg gccagacatg
      541 ccgccgttat gagaatggta gtgactttca tcatggaata ggggaatgca aaattaatat
      601 acggagctcc cccggcgcca tctttcattc atataataca tccttgcgta taatgcaaat
      661 acatatgtca tccggcatct tccgcttgac ctggaaggca tgttttttca tagtcgattc
      721 gccctgtacg ggattatccc gcagccccgg tccgagtgcc aggctttcag tcccgtgaaa
      781 aggaaaaagc gaggcgttcc tccaccggct gtctcagggc tatgccttta tccactagtt
      841 ttttatgtca tctttctttc agaaaattgt caaacttttc tcctatgaca ttggcattga
      901 cctgggtacc gccaacaccc tggtcaacgt caaggaccag ggcatcgttc tgcgcgagcc
      961 ttcggtcgtc gccgtcaagg gggacaaggt tctggccgtg ggagacgaag ccaagcgcat
     1021 gctgggccgc acccccggct ccgtcacggc catccggccg ttgaaggacg gcgtcatcgc
     1081 cgacttctac atcacggagg aaatgctccg ctacttcatc aaaaaagcca cgtccaggta
     1141 ctacctgatc aagccgcgcg tgctcattgc cattccctcc ggcattacgg aagtggaacg
     1201 cagggccgtc aaggaatccg cggaagcggc cggagcccgc cgcgtgcatc tgattgaaga
     1261 acccatggcc gccgccatcg gtgtaggcct tccggttcag gaagctgcgg gcaacatgat
     1321 tgtggacatc ggcggcggta cgacggaagt ggcgcttatt tccctttccg gcatcgttta
     1381 cagccgttcc gtccgctgcg ccggggatga actggacgac gccatcatct cctacatgag
     1441 gagaacctat aatctcatgg tcggggaacg tactgccgaa gacatcaaaa tccgtatcgg
     1501 ctccgcctat cccctgcctc aggaactctc tatcgaggtc aaaggccgcg acctggtcgc
     1561 gggcctgccc aagacagtga cgattcgatc ggaagaagtg cgtgaagcgc tcacggaaca
     1621 gctcaacacg attgttgacg ccgtgcggac gactttggaa cgttgccccc ctgaactggc
     1681 tgccgacctt gtggaccggg ggattgttct ggctggagga ggagctctgc tgcgcgggct
     1741 ggatcagctc ctgcatgaac agacgggact gcccatccac attgcggaag accctctgag
     1801 cgccgttgcg gaaggtaccg ggaaagccct ccaggaactg gacttcttca ccaatgttac
     1861 tgatgcggaa gactaaagac ggaatctccg gagcaagcgc tccggccttg cgtctgccgt
     1921 actgccgcat cacatcaacg caactgcctg catgattcct ttccggccat ccctacagct
     1981 tcaactccag ccgctcctga cgggagacgg cccaccggaa acattacggg caatagcaca
     2041 ccctagctta ttctcacccc gtgtttacaa aaatgcttaa aaaaatcaaa cgggccttcg
     2101 gcttcggatc ctgcgatccc atggaaggct ccactatcat catcaatgcc gaaaagctcg
     2161 aacgccgcgt ggcccttctg gaaaacggcg tattggaaga atacaccatt gagcgcgaag
     2221 gcgaagacaa catcgtaggc ggcattttca aaggccgcgt caagaacatc gaaccgggcc
     2281 tcaaagccat gttcgtggac attgggcagg aaaaaaacgc ctttctgcac ttctgggacg
     2341 cgcttcccgc cgcactggac tccagcctgg aagaaatcga acgcgaaggc cgtccgaaaa
     2401 aacagcagcg caaaatcacg tccaaagaca tcccggacat ctatcccatc ggctctgaaa
     2461 tcatggtgca ggtcaccaag ggaccgatcg gcaccaaggg tccccgtgtc acaacgaacg
     2521 tatccctggc tggacgctac ctggtcctga tgccctttac ggaccagttc ggcatctccc
     2581 gtaaaattga agaccccaaa gagcgcctgc gcctgcgcca gatcatgcag aaactgaatg
     2641 ttccggatgg aatgggcatc atcatgcgca cggtggcaca gggccagcgc taccgccact
     2701 ttgtccg
//
LOCUS       DAOMSU010000287         2706 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3877_length_2706_cov_2.851754, whole genome shotgun sequence.
ACCESSION   DAOMSU010000287 DAOMSU010000000
VERSION     DAOMSU010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3877_length_2706_cov_2.851754"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            227..1117
                     /locus_tag="PK077_09660"
     CDS             227..1117
                     /locus_tag="PK077_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24603.1"
                     /translation="MAIRYCSFATRGYHLRQWMQMMSLRLADSSVELSCWNLNRLERE
                     GIREAIPWFDPNLQGAGFWVWKPFIILKELEHLEEGDYLLYTDAGRPPGRLFHHSLEP
                     FVQWLREKGQDMMPGVNISYYTGGLTRWCKQSVFDELKLDSGSFEQAPLIQGSFLFLK
                     KSEQSLRILREWMDLCRQFKLVSSATTEEESRCRDNFVAHTHDQTLLSLVAAQHHLDC
                     LYSGKGVIHLEGRRAFERHPGFDDKDPDWFLEELGAKKAEHPLMPVLCCMQQAFFLYE
                     KAMNKLMKLAGLRPLRGFYG"
     gene            complement(1249..>2706)
                     /locus_tag="PK077_09665"
     CDS             complement(1249..>2706)
                     /locus_tag="PK077_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HRN24604.1"
                     /translation="GMVFDRSYCANSLCGPSRACILTGRHSHMNGFVFNGQRPLDGSQ
                     PTYPKMLQKAGYQTGLFGKWHLESDPTGFDTWEIFPGQGSYYNPDFISLKPDGKRQTK
                     RFPGYATDVVTDKSIQWLENRDKNKPFLLVVGHKAPHRAWCPALRHLGKVDTSGMTPP
                     ANFHDDYANRPEFLKKNQQTVANHMAIYSDLKVLKDQVPEEMRKSIVSPGYGWDLGEL
                     NRMTPEEKKTWTDYYAKRTKSLVDGMKSGKLKDPKAFAEWKWHAYMEDYLGCLLSVDD
                     SIGRLMEYLDKEGIAKDTLVIYCGDQGFYMGEHGMYDKRWIFEESLRMPLIMRWPGKI
                     PAGIRNNTMVQNIDYAPTIVSAAGADTPENMNTFQGVSLLPTAFTGKTPDNWRDAIYY
                     CFYENPGEHNAPRHDGIRTDRYTLSYIWTSDEWMLFDMKKDPMQMKNVIDDPAYKTTV
                     EQLKKRYHELRKTYKVPENSPGGKGTPIPKFDASW"
BASE COUNT          546 a          636 c          807 g          717 t
ORIGIN      
        1 taaaaaagtt tggatatagt tataaaaaaa taactataaa taacttatcg tgttgaatgg
       61 ttttttggta attaaaaaaa tcctgtttca atccgtggtt ccatgcacgg agatgattct
      121 ccgttaaagt tttccattga cagcggattt acaactcgtt ataggtacgg acgcagaaga
      181 tgtgttcccg tttttaggga catggtttcc accggaatct gggagtatgg ctatccgtta
      241 ttgttctttc gcgacccgcg gctatcattt gcggcaatgg atgcagatga tgagcctgag
      301 gctggcggat tcctcagtgg agttgtcctg ttggaatctt aatcgcctgg aaagggaagg
      361 aatcagggag gctattccat ggtttgaccc gaatttacag ggtgccgggt tttgggtatg
      421 gaaacccttc atcattctca aggagctgga acatttggag gaaggggatt acctgcttta
      481 tacggatgcg gggcgcccgc cggggaggct tttccatcat tccttggagc cgtttgtcca
      541 atggctccgg gagaaggggc aggatatgat gccgggcgtg aatatttcct attatacggg
      601 aggccttacg cggtggtgca agcagagcgt ttttgatgaa ttgaagctgg atagcgggtc
      661 ttttgaacag gctcccctga ttcagggctc cttccttttc ttgaaaaaat cggaacaaag
      721 cctccgaatt ctccgtgaat ggatggattt gtgccgtcag ttcaaattgg tgtcttctgc
      781 cactacggaa gaagagagcc gttgccgcga taattttgta gctcatacgc acgatcagac
      841 gctgctgtct ctcgtggcgg cgcaacatca tctggactgc ctgtattccg ggaagggcgt
      901 cattcatctg gaaggccgcc gggcgtttga gcggcatccc ggttttgacg acaaagaccc
      961 ggactggttt ttggaagagc ttggcgcaaa gaaagcggag catcccttaa tgcccgtgct
     1021 ttgctgcatg caacaggcgt ttttcctgta tgaaaaagcc atgaataaat tgatgaagct
     1081 ggcgggcttg agacccctcc gcgggtttta cggttgatgg acggttgtgt gcggaagggg
     1141 agtttgcctg tttcggcctc cgtgacggat ctctccggcc tgctatggac gaaaataaaa
     1201 aagcgccgga aaaaccggcg cttggaaagg tagtttcccg tgtggctttt accaggaagc
     1261 gtcgaatttg gggataggcg ttcctttgcc tccggggctg ttttccggaa ctttataggt
     1321 tttgcgcagt tcgtggtaac gcttcttgag ctgttccacc gtagtcttgt aggcaggatc
     1381 gtcaatgacg tttttcattt gcatgggatc ctttttcatg tcaaagagca tccattcgtc
     1441 gctggtccag atgtaggaaa gcgtgtagcg gtccgtccgg atgccgtcgt ggcgcggggc
     1501 gttgtgttcg ccgggatttt cgtaaaaaca gtagtaaatg gcatccctcc agttgtcggg
     1561 agttttgccc gtgaaagcgg tgggaagcag ggatacgccc tggaaggtat tcatgttttc
     1621 cggggtgtcc gcccctgccg cagaaacgat ggtgggagcg tagtcgatat tctgcaccat
     1681 ggtgttgttg cggatgcccg cgggaatttt gccgggccat ctcatgatga ggggcatgcg
     1741 gagggattct tcaaaaatcc agcgcttgtc gtacatgccg tgttctccca tgtagaaccc
     1801 ctggtctccg cagtagatga ccagcgtgtc tttcgcaatc ccctctttgt ccagatattc
     1861 cataaggcgg ccgatgctgt cgtccacgga cagaaggcat cccagataat cctccatgta
     1921 ggcatgccac ttccattccg caaacgcttt cgggtccttc agttttccgg atttcatgcc
     1981 gtccaccagg gatttggtgc gcttggcgta atagtccgtc caggttttct tttcttccgg
     2041 agtcatgcgg ttcaactcgc ccaggtccca gccgtaaccg ggggaaacga tgcttttgcg
     2101 catctcttcc ggaacctggt ccttaagcac tttgaggtcg gaataaatcg ccatgtgatt
     2161 ggcgacggtc tgctggtttt tcttcaggaa ttccggacgg ttggcgtagt cgtcatggaa
     2221 gttggcgggc ggcgtcatgc cggaggtgtc caccttgccc aggtggcgca gagcagggca
     2281 ccaggcgcgg tggggagcct tgtggcccac aacgagcagg aaaggcttgt tcttgtcccg
     2341 gttttccagc cactggatgg atttgtccgt gaccacgtcc gtggcatatc cgggaaaacg
     2401 ctttgtctgg cgtttgccgt ccggcttgag gctgataaag tccggattgt agtagctgcc
     2461 ctggccgggg aagatttccc acgtgtcgaa cccggtgggg tccgattcca ggtgccattt
     2521 gccgaaaagg cccgtctggt atccggcctt ctgcagcatt ttcgggtaag tgggctggga
     2581 gccgtccagc ggtctttgtc cgttgaagac aaagccgttc atgtgggaat gcctgccggt
     2641 caggatgcag gcgcgggagg ggccgcacag ggagttggcg cagtagctgc ggtcaaaaac
     2701 catgcc
//
LOCUS       DAOMSU010000288         2704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3885_length_2704_cov_2.299358, whole genome shotgun sequence.
ACCESSION   DAOMSU010000288 DAOMSU010000000
VERSION     DAOMSU010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3885_length_2704_cov_2.299358"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            21..674
                     /locus_tag="PK077_09670"
     CDS             21..674
                     /locus_tag="PK077_09670"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HRN24605.1"
                     /translation="MVFDYKKEYKEFYMPKNKPAIVTIPKMNYIAVRGKGNPNDEGGE
                     YQRSIELLYGIAYTIKMSYKSSRKIKGFFEYIVPPLEGFWWQNGSDGMDYGHKENFKF
                     ISIIRLPDFVTKEDLEWAIEEATKKKKKDFSTVDFLTYEEGDCVQCMHLGSYDNEPET
                     IHQMEEYANANGYEIDITDTRYHHEIYLSDPRKCDVGKLKTVIRHPVKRKQKILEKK"
     gene            complement(1180..2574)
                     /gene="lpdA"
                     /locus_tag="PK077_09675"
     CDS             complement(1180..2574)
                     /gene="lpdA"
                     /locus_tag="PK077_09675"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRN24606.1"
                     /translation="MQYDLIVIGGGPAGYVGAIRAAQLGKSVVCVERERVGGTCLNWG
                     CIPTKALLKNAEAYLTVTARAREFGMTVEGVSVDWSEVIGRSRKISDRLAGGVGFLFK
                     KNKVDSVTGEASIISPGRVEVKAADGTVNVLEGKNILVCTGCVTRTVPSLPLNGTTVI
                     GSREAMVLEKRPESMIIIGSGAIGTEFAYIYNSFGTRVTLIEALPRMLPNEDDDSCMT
                     LERAFKKQGIKVMTGASVESVTETCDGQVKASVKNSRGQEEEITADICLVAIGVKPVV
                     PAAPGLDLELTEKGFIKVNDRYATSIPGVYAAGDVIGGVLLAHTASFEAVQAVEGMFN
                     PDYQPRQVGFFPSCTYCYPQVASVGKTERALKEAGVEYKVGKFPFQAIGKAVAAGEPD
                     GFVKTLYGAKNGELLGAHIVGPEATELIAALGIGIQAELTDEDIHATIFAHPTLSEAI
                     HESMLASEGIAIHM"
BASE COUNT          670 a          715 c          661 g          658 t
ORIGIN      
        1 aatttcaatg agtgttaagc atggtttttg attataagaa agaatacaag gaattttaca
       61 tgccgaagaa caaaccggca atcgttacta ttcctaaaat gaattacata gcagttcgtg
      121 gcaagggaaa tcccaatgat gagggtggag aataccaacg ctccattgaa ctactttacg
      181 gaattgccta tactatcaaa atgagctata agagttcccg caagattaaa ggcttttttg
      241 agtatattgt cccgcctctt gaaggctttt ggtggcaaaa tggcagcgat ggaatggact
      301 atggccataa agaaaatttt aaatttattt ctattatccg tttaccagac tttgtgacta
      361 aggaagatct tgagtgggca atcgaggaag caaccaaaaa gaaaaagaag gacttttcaa
      421 cggtggattt tcttacctat gaagaaggcg attgcgttca atgcatgcac ctcggctctt
      481 atgataacga acccgaaacc atccaccaga tggaagaata cgcaaatgcc aatggatatg
      541 agatcgatat taccgatact cgttatcatc acgaaattta tctttccgat ccacgcaaat
      601 gtgatgtggg caaactgaaa actgtcatca gacacccggt aaagagaaaa cagaaaatat
      661 tggagaagaa ataaatgcga ggaattatct atacgttata aatatatttt ccccataaat
      721 tagtatcaaa cacttcccat tcatcacgca aataaaaaac gaaaacagag gctgaagtgg
      781 aactgtttcg gaactgtttc caaaatcttt ttgccctttc ttttccgtac ttcaatgcgg
      841 catgagccgt tttggcttac tcccgctccg ttttcgcggc tgtccgtcca ctcgtgccgc
      901 aaatggccct gttgcctgtg gtttccaggt tatccccgtt ctttgatgat ataaacaagc
      961 aaaccgcggc tttccaaaac ttacagtcaa tctactacat tgactatcaa tcctgcttaa
     1021 agcctgtctg ataaacttac gggaattgtt gacgctgcag ccctcatcaa caagtttatc
     1081 cttgaacgtc ttttttcaac gcagattgtc attcacccgt ttgcatgaca aaggaatgaa
     1141 tggaagagcc ccggatgggg gcccttccat tgaaaaccgt tacatgtgga tggcgattcc
     1201 ctcggaagcc agcatggatt catggatggc ctcagacagc gtgggatggg cgaaaatggt
     1261 ggcgtggatg tcctcgtccg tcagttccgc ctgaatgccg atgcccagcg cggcaatcag
     1321 ctccgtggct tccggcccca cgatgtgggc gcccaggagt tctccgtttt tggcgccgta
     1381 cagggttttt acgaaaccgt ccggttctcc ggcggctaca gccttgccaa tggcctggaa
     1441 ggggaatttc cccaccttgt attccacgcc cgcctctttg agggcgcgtt ccgttttacc
     1501 tacggaagcc acctgcgggt agcaatatgt gcagctcggg aaaaagccca cctgcctggg
     1561 ctgatagtcc ggattaaaca tgccctctac tgcctgcaca gcttcaaaag aagccgtatg
     1621 cgccagcagc acgccgccga tcacatcgcc tgccgcatac acgccgggta tggaagtcgc
     1681 atagcggtca ttcaccttga tgaatccctt ttccgtcagc tccaggtcca ggccgggcgc
     1741 ggcgggaaca accggcttca cgccgatggc caccaggcag atatccgccg tgatttcctc
     1801 ttcctgtccc ctgctgtttt tcacgctggc cttgacctgt ccgtcacagg tttccgtaac
     1861 ggattccacg gaggctccgg tcatcacctt gatgccctgc ttcttgaatg cgcgttccag
     1921 cgtcatgcag gagtcgtcgt cttcattggg aagcatgcgg ggaagcgctt caataagcgt
     1981 taccctggtg ccgaatgaat tgtaaatgta ggcaaattcc gtgccgatgg ccccggaacc
     2041 gatgatgatc atgctttcag gccgcttttc cagcaccatg gcttccctgg agccgatgac
     2101 ggtagtgccg ttcaggggga ggctgggcac cgtgcgggtg acgcagccgg tacagaccag
     2161 gatattcttt ccctccagaa cattcaccgt cccgtcggcg gcctttactt ccaccctgcc
     2221 gggagaaatg atggaggctt cccccgtaac ggaatccacc ttgtttttct tgaacaggaa
     2281 cccaacgccc ccggccaggc ggtcgctgat cttgcgggaa cggccgatca cttcgctcca
     2341 atccacgctg acgccctcca ccgtcatgcc gaattccctg gcacgggccg ttacagtcag
     2401 gtaggcttcc gcgtttttca gaagcgcctt ggtggggatg caaccccagt tcagacaggt
     2461 gccccctacc cgctcgcgtt ccacgcacac cacggatttc cccagctggg cggcgcggat
     2521 ggcgcccaca tagccggcag ggcctccacc aatgacgatg agatcatatt gcatgaaatt
     2581 actataaagg catcccccca tgcgggtaaa gaaggaatat gagcatgcgc cggttcggga
     2641 ggccgacaga tagcagaggt aagggaaggg tgattaaagc atgtgttgaa aacctacctt
     2701 tatt
//
LOCUS       DAOMSU010000289         2700 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3893_length_2700_cov_2.482420, whole genome shotgun sequence.
ACCESSION   DAOMSU010000289 DAOMSU010000000
VERSION     DAOMSU010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3893_length_2700_cov_2.482420"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="PK077_09680"
     CDS             <1..321
                     /locus_tag="PK077_09680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24607.1"
                     /translation="RLGTGLRYSATLCYNTFPLRELTEGEKARVTEAALGVLDAREAH
                     PEKTPAELYDPDRMPENLRKAHDRLDAEVDRLYRVKGFKDDGERLECLFELYREMTQG
                     QQTE"
     gene            327..2273
                     /locus_tag="PK077_09685"
     CDS             327..2273
                     /locus_tag="PK077_09685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24608.1"
                     /translation="MNVMQVNYGATGASTAVDALGMRDMQARAYAKRRERYLLLKAPP
                     ASGKSRALMYLALDKLERQGLRKVIVAVPERTIGGSFAPVRLTENGFFADWTPDLRYN
                     LCTPGADAGKVARFVEFMESEARILICTHATLRFAFEKLATEAFSGCLLGIDEFHHVS
                     ADRESSRLGALLHDVMRETDAHVLAMTGSYFRGDNVPILLPEDEARFSRVVYTYYDQL
                     NGYRHLKSLGIGYHFYQGRYLDALPEVLDTDRKTILHIPHVNSGESTKEKYDEVGHIL
                     DIMGTVEGEDPENGLLYVRRRADGRVLKVADLVNDEAAHRNRLVAYLRKVKQKDELDI
                     IIALGMAKEGFDWPWCEHALTVGYRGSLTEIIQIIGRATRDCPGKTHAQFTNLVAAPA
                     AKDEEVTLAVNNMLKAVTASLLMEQVLTPEFKFRARAESEDERRPGTIHIRGFKEPGT
                     GRVRDIVENDLADLKARIMQDDTVRKLIAHPDADPRIVNKVLVPKIIAERYPGLTEEE
                     REEVRQAVVTDCAIRSGEIREEKDSVSGQSREFIKMGEKFVNVNDLDINLIDGINPFR
                     GAFEIMSKQLTAPVLRLIRDAIAETRITLGLQEAVLLWESVLAFNKARGREPDPNAED
                     ANEATLGQALVLIRREKRRRARHG"
     gene            2266..>2700
                     /locus_tag="PK077_09690"
     CDS             2266..>2700
                     /locus_tag="PK077_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24609.1"
                     /translation="MDKEALERRLAAIFEEAEEAGIHLDSKPERVPVSRGDRLAESFE
                     EISRFVDECGREPVKGLSMNERRLAARLEQIRQDPVRAAQLRPIDRHGLLPQPPATVE
                     EVFHDKSMEALLEDEDAADIFDVSALPRRELPEYIAKRVPCRN"
BASE COUNT          604 a          659 c          935 g          502 t
ORIGIN      
        1 cgtttaggga caggacttcg ttattccgcc acgctttgct acaacacttt tccattgagg
       61 gaactgacgg agggagaaaa ggcgcgggtg acggaagcgg cgctgggggt gctggacgcg
      121 cgggaggcgc atccggaaaa gacgccggcg gagttgtatg atccggacag gatgccggag
      181 aacctgagga aggcgcatga ccggctggat gcggaggtgg accggcttta ccgggtgaag
      241 ggctttaagg atgacgggga acggctggaa tgcctgtttg agctgtaccg ggaaatgacg
      301 caggggcaac aaacggaata agaaacatga acgtgatgca ggtcaactac ggggccacgg
      361 gagccagcac ggcggtggac gcgctgggga tgcgcgatat gcaggcgcgc gcgtacgcca
      421 aaaggcgtga gcggtatctg ctgctaaagg cgccgccggc atccggcaag agccgcgcgc
      481 tgatgtatct ggctctggat aagctggaac ggcaggggct gcgcaaggtg attgtggctg
      541 tgccggaacg gacgattggc ggttctttcg cgcccgtacg gctgacggaa aacggatttt
      601 tcgcggactg gacgccggac ttgcgctaca acctgtgcac gcccggagcg gatgccggca
      661 aggtggcccg ttttgtggag tttatggaga gcgaggcccg catcctgatt tgcacgcatg
      721 ccacgctacg cttcgcgttt gaaaagctgg ctacggaagc gttcagcggc tgcctgctgg
      781 ggatagatga atttcaccat gtgtccgcgg accgtgaaag cagccggctg ggcgctcttc
      841 tgcatgacgt gatgagggaa acggacgccc atgtgctggc catgacgggc tcctacttcc
      901 gcggggacaa tgtgcccatt ctgctgccgg aagatgaagc caggttcagc cgggtagtgt
      961 atacgtatta tgaccagctg aacgggtaca ggcatttgaa atccctgggg ataggctatc
     1021 atttttacca gggccgttat ctggacgccc tgccggaggt tctggatacg gacaggaaga
     1081 ccatcctgca cattccccat gtgaacagcg gggaatccac caaagaaaag tatgatgaag
     1141 taggccatat tctggatatc atgggcacgg tggagggaga agacccggag aatggcctcc
     1201 tttacgtgcg ccgccgcgcg gacgggcgcg tgctgaaagt ggcggatttg gtgaatgatg
     1261 aggccgccca ccgcaacagg ctggtggctt atctgcgtaa agtgaaacag aaggatgaac
     1321 tggatattat cattgccctg ggcatggcca aggaaggctt tgactggccc tggtgcgaac
     1381 atgccctgac ggtgggttac cgggggtctt tgacggagat catccagatt atcggccgcg
     1441 ccacgcgcga ctgcccgggc aagacccatg cccagttcac caatctggtg gccgccccgg
     1501 cggcgaagga tgaggaagtg acgctggcgg tgaataatat gttgaaagcg gtgacggcct
     1561 ccctgctgat ggagcaggtg ctgaccccgg aattcaaatt ccgggcccgt gcggagagcg
     1621 aggacgaacg caggccgggg acgattcaca tccgcggatt caaagaaccg ggcaccgggc
     1681 gtgtgaggga tatcgtggag aatgacctgg cggatttgaa agcccgcatc atgcaggatg
     1741 atacggtcag aaagctgatt gcccatccgg atgcggaccc ccgcatcgtc aataaggtgc
     1801 tggtgcctaa aatcattgcg gaacgctatc ccggattgac ggaagaggaa cgggaagaag
     1861 tgcgtcaggc cgtggtgacg gattgcgcca tcaggagcgg ggaaatcagg gaagagaagg
     1921 attccgtgag cggtcagagc cgggaattta ttaagatggg tgaaaaattt gttaatgtta
     1981 atgatttgga tatcaacctg atagacggaa ttaatccctt ccgcggggca tttgagatta
     2041 tgtccaagca actgacagcc cccgttttgc ggctgatacg ggatgccatt gcggaaacgc
     2101 gcatcaccct ggggctgcaa gaggccgttc tgctgtggga atctgtgctg gctttcaaca
     2161 aggcgcgcgg cagggaacct gacccgaacg cggaggatgc caatgaagcc actttggggc
     2221 aggccctggt gctgatacgc agggaaaaac ggaggagggc gcggcatgga taaggaagcg
     2281 ctggaaagaa ggcttgctgc catttttgag gaggcggagg aagccgggat ccacctggac
     2341 agcaagccgg agcgagtccc tgtgagcagg ggggacaggc tggcggagtc ttttgaggag
     2401 atctcccgtt ttgtggatga atgcgggagg gaaccggtca aggggctatc catgaatgaa
     2461 cgcaggctgg ccgcccggct ggaacaaatc cgtcaggatc ccgtcagggc ggcccagctg
     2521 cgccccattg accggcacgg gctgctgccc cagccgcctg ccacggtgga ggaggtgttc
     2581 catgataaga gcatggaggc tctgctggag gatgaagacg ctgcggatat ttttgacgtg
     2641 agcgctctgc cccggcggga gctgccggaa tatattgcca aacgcgtgcc atgccggaat
//
LOCUS       DAOMSU010000290         2686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3919_length_2686_cov_2.755606, whole genome shotgun sequence.
ACCESSION   DAOMSU010000290 DAOMSU010000000
VERSION     DAOMSU010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3919_length_2686_cov_2.755606"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            214..543
                     /locus_tag="PK077_09695"
     CDS             214..543
                     /locus_tag="PK077_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24610.1"
                     /translation="MKKLIYMIAAAAVAIPVVALAQGACGSGSCSKGDKDMAEAKEHM
                     KEGAKAAKDAASEKMKEGRQAARDAYEKGKETARDAYQDSRDAVDHAADRAADKAEAV
                     GDAVKNG"
     gene            599..766
                     /locus_tag="PK077_09700"
     CDS             599..766
                     /locus_tag="PK077_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24611.1"
                     /translation="MNKGCGCANDAASGKMEEGKHKMKDACERGKEAVKDMAESVSDK
                     AKSIMDKDKNS"
     gene            complement(858..1721)
                     /gene="lpxI"
                     /locus_tag="PK077_09705"
     CDS             complement(858..1721)
                     /gene="lpxI"
                     /locus_tag="PK077_09705"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.2"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HRN24612.1"
                     /translation="MTTDPPVLGLVAGDGVYPEYIVRGARRRTPELRIVAVGFKGETN
                     PAVIPLCDAYQEFSVGQISKPFTFLKKHGVRNVIMAGGINPKNILSLRPDLRALSVLM
                     RMPEKNADSLLGAVITEAEKEGFTILPASTYMEEHMPQPGHIAGPPPTPEQWEDARFG
                     MQTAKEISRLHIGQSVIVHGGTVIAVEAIEGTNNCIRRGGELGNGKPATLAKVARLGH
                     DMRFDIPTVGPVTIETCAECGVRQIALEAGKTILLERDRVEELCKRHKISLHALQTVE
                     QDTPSPENRPA"
     gene            complement(1750..>2686)
                     /locus_tag="PK077_09710"
     CDS             complement(1750..>2686)
                     /locus_tag="PK077_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HRN24613.1"
                     /translation="ASAREEKPAGVHFGLTGESYQENGTDLKVQPSVRGLPVPAGMKG
                     AHYMSPRMKARMDELGMSASDIAFISGSGAGGRVTIDDLEEFLEYVSQWPHRKASSMR
                     LAVADAMRRSWTRPLASAGRPVFMDPLIKHRQNSPLRPGITLYFARALALALAESPEC
                     AGYLVGENILSPKTIDIGIAAQVADGVMVPVLRRVNERTMEELLEDYNRLIAQARRRR
                     LAPEDSTGGIATVTNFGGFGLTFAAPMPMPSESIILGVGAVTKTPVWSDEVEAFIPIS
                     KANIVATGDHRVVDGADIGRLLKRVAELLQRPEYL"
BASE COUNT          531 a          737 c          880 g          538 t
ORIGIN      
        1 agggaggagc tgaggcggcg gtttgtggaa aagctgggct attcccattg cagggagttg
       61 aaaaaagagg ggtccgtgcc atgccgggat tgcgttgcct ctgcggcggt tctggctttt
      121 aacctgaggg agtgaacgca gaatgacact gccggattca tggtttttca tgaaaaaaga
      181 gtctattgga atgaaaccag atagaataac atcatgaaaa aattgatcta tatgatagcg
      241 gcagcggccg ttgcgattcc ggtggtggcc ctggcccagg gagcctgcgg cagcggctcc
      301 tgctccaagg gagacaagga catggctgaa gccaaagagc acatgaaaga gggcgccaag
      361 gctgccaagg acgccgcctc cgaaaaaatg aaggaaggca ggcaggctgc cagggacgcc
      421 tatgaaaagg gcaaggaaac cgccagggat gcttatcagg acagcaggga cgccgttgac
      481 catgcggcgg acagggctgc cgataaggcc gaagccgtcg gtgacgctgt gaaaaacggt
      541 taactcaaac gatgaacaat aaaaatattt gcttattatg tgtgaatcca aggatcatat
      601 gaataaaggc tgcggatgtg cgaacgatgc cgcttccggc aaaatggagg aaggcaagca
      661 caagatgaaa gacgcctgcg aaagaggcaa ggaagccgtc aaggacatgg ctgaaagcgt
      721 gagcgacaag gccaaatcca tcatggacaa ggataagaat tcttagtccc ggcggcggtt
      781 gaagcaagcc gtttcccatt cttcctcccg tgcgcgggat gacctcccct tgtccggatt
      841 tccggacgag gggattttta tgcgggccgg ttttccgggg aaggggtgtc ctgttccacg
      901 gtctggaggg catgcaggct gattttatgc cttttgcata attcctccac ccggtcgcgt
      961 tccagcaaga tggttttgcc cgcttccagg gcgatttgcc tgacgccgca ttccgcgcac
     1021 gtttcaatgg tgacggggcc gactgtcggg atgtcaaagc gcatgtcatg ccccaggcgg
     1081 gccactttgg ccagcgtggc cggtttgccg ttgcccagtt cccctccgcg gcgtatgcag
     1141 ttgttggtgc cttcaatcgc ttccacggcg atgacggtgc cgccgtgcac gatgacggac
     1201 tgcccgatgt ggagacggct gatttccttg gccgtctgca tgccgaagcg ggcatcctcc
     1261 cattgttcag gcgtgggagg cggtccggcg atatgccccg gctgcggcat gtgctcctcc
     1321 atgtaggtgg aggccgggag gatggtgaag ccttcctttt ccgcttccgt gatgacggcc
     1381 ccaagcaggg agtccgcatt tttttccggc atgcgcatca atacggaaag ggcgcggaga
     1441 tccggacgga gggaaagaat attcttcgga ttgatgccgc cggccatgat gacgttcctt
     1501 acgccgtgct ttttcaggaa agtgaacggc ttgcttatct ggcccacgct gaattcctgg
     1561 taagcgtcgc acagggggat gacggcgggg ttggtttccc ccttgaaccc tacggccacg
     1621 atacgcaatt ccggcgtccg gcggcgtgcg ccccggacaa tatattccgg ataaacgccg
     1681 tctccggcta ccaggcctaa tacgggcggg tctgttgtca tgcggcgtga agctggggaa
     1741 cggggctggt tacaggtatt ccgggcgctg gagcaattcc gccacgcgct tgagcaggcg
     1801 gccgatgtcc gctccgtcca ccacgcggtg gtctcccgta gccacgatgt tggccttgga
     1861 gatggggata aacgcctcca cttcgtcact ccagaccggg gttttggtga cggcccccac
     1921 ccccaggata atggattcgc tgggcatggg catgggcgcg gcaaaggtga ggccgaagcc
     1981 gccgaagttg gtgaccgtgg cgatgccgcc cgtgctgtct tccggagcca gcctgcggcg
     2041 gcgcgcctgg gcaatcagcc tgttatagtc ttccagcagt tcttccatcg tccgttcgtt
     2101 cacgcggcgc agcacgggaa ccatgacgcc gtccgccacc tgggcggcga tgccgatgtc
     2161 gattgttttg ggagaaagaa tgttttcccc caccaggtag ccggcgcatt ccgggctttc
     2221 cgccagggcc agggccaggg cgcgggcaaa gtacagggtg atgccgggcc gcaacgggga
     2281 attttgcctg tgcttgatga gggggtccat gaatacggga cgcccggcgg aggccagcgg
     2341 gcgcgtccag ctgcggcgca tggcgtccgc cacggccagc cgcatggatg aggccttgcg
     2401 gtgcggccat tggctgacat attccaggaa ttcttccagg tcgtcaatgg tgacgcgtcc
     2461 gcccgccccg gaaccggaga tgaaagcgat gtcggaggcg ctcatgccca attcatccat
     2521 gcgcgccttc atccgggggg acatgtagtg cgcgcccttc atgccggcgg gtacggggag
     2581 gccgcgcacg ctgggctgga ctttcaggtc cgttccgttt tcctgatagg attcccccgt
     2641 caggccaaag tggaccccgg cgggcttttc ctcccgggcg gaagcg
//
LOCUS       DAOMSU010000291         2685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3923_length_2685_cov_1.828517, whole genome shotgun sequence.
ACCESSION   DAOMSU010000291 DAOMSU010000000
VERSION     DAOMSU010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3923_length_2685_cov_1.828517"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(385..2388)
                     /gene="tkt"
                     /locus_tag="PK077_09715"
     CDS             complement(385..2388)
                     /gene="tkt"
                     /locus_tag="PK077_09715"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRN24614.1"
                     /translation="MNLDLLQKAANQARGLAMDAVHDCASGHLGLPLGCAEIGAVLFG
                     DLLNICPSQPRWLNRDRFILSAGHGSMFLYGWLHLSGFNIGIEDIKNFRHKGSITPGH
                     PEFRDTEGVECTTGPLGQGIANAVGFALSARRAAARFNRPGMDIFTQHVFCLTGDGCL
                     QEGVARESLALAAVLKLDNLILIYDSNDITLDAPAERTQLTDPRAVYEALGWDVRQID
                     GHDIKAIKSAVEAAKNAKNGKPQLIIAKTVIGKGIPGIEGTTKGHGEGGAKLQEEAHA
                     NWEIPAGERYYVSEDVRTAFADLKAQREKDFNAWNAMYEQWRRAYPELAEELDAGINA
                     CSCGVNPADSDKAIPPFPQDYGDATRSAGAVAINAIAKANPCFLTTSADLYSSNKNYL
                     SGAGDFSAETPEGRNFWFGIREHAMAAICNGIAYDGLFRVSAATFCVFVDYMRASIRV
                     AALSGLPVTYILTHDSVAVGEDGPTHQPVETISGLRVIPNLDVIRPADPEETAGAWMA
                     AMQRADGPTALILTRQKVATLNGIPVETRREGVLKGAYIARKEQGPLKAIILASGSEL
                     ELALKAAEKIGEGIRVVSMPSFCRFDAQPAEYRESVLPSSCMRRVSVEAGVTDLWWKY
                     LGCQGEAVGINRFGFSAPGTQVLEELGMNVDNVVAAVHKVLAR"
BASE COUNT          511 a          718 c          868 g          588 t
ORIGIN      
        1 gggttcattg tatcgtcaat agaggcggcg cttgatcatg gccgccccgc atatttaccg
       61 gcgccatgaa ggaaacctga aattatccgt tccggcagct gagatgaaaa cgggctgctt
      121 gcaaccgggg cttttgcggc ctgagtccca tccgggtccg gttcggacaa tggagctgat
      181 gtgcggaggt cttgtattta gagaaatccg gacgggaaac gaacggaatt ttcacaggaa
      241 aggatatagt tcctgcgtta cggatactgg tgtttcgtga aaacggcctt gcctttttcc
      301 tggcaaccgg ggaggcatga aaaaggggct gtcccggcgg atgcggaaca gcccctgaaa
      361 ggagatgaag ggaaaattcc ctgtttatct ggcaagcact ttgtgaacgg cggcaacgac
      421 gttgtccaca ttcatgccga gttcctccag cacctgcgtt ccgggagcgg agaagccgaa
      481 acggttgatg cccacggctt ccccctggca gcccagatat ttccaccaga ggtccgtgac
      541 tccggcttct acggaaactc tcctcatgca ggaagaggga agcacgcttt cccggtattc
      601 ggcaggctgc gcgtcaaagc ggcagaagct tggcatggag acgacccgga ttccttcccc
      661 tattttttcc gccgctttca gggccagttc cagttcagaa ccactggcaa gaataatggc
      721 tttcaggggg ccctgttcct tccgggcgat gtaggctcct ttcagcacgc cttcccggcg
      781 cgtttcaacg gggattccgt tcagcgtagc cactttctga cgggtcagga tgagggcggt
      841 agggccgtcg gcacgctgca tggcggccat ccaggctcct gcggtttctt ccgggtccgc
      901 cgggcggatg acatccaggt tcggaatgac acggaggcct gaaattgttt ccaccggctg
      961 gtgggtgggg ccgtcttctc ccacggctac ggagtcatgc gtcagaatat aggtgacggg
     1021 gagtccgctg agggcggcta cacggatgga ggcgcgcatg tagtctacga agacgcagaa
     1081 agtggcggcg cttacgcgga acaggccgtc gtaggcaatg ccgttgcaga tggcggccat
     1141 ggcgtgttcg cggatgccga accagaagtt gcgcccttcc ggggtttccg cagagaagtc
     1201 tcctgcgccg gaaaggtagt ttttattgga gctgtaaagg tctgcgctgg tggtcaggaa
     1261 gcagggattc gctttagcga tagcgttgat ggcaacggct ccggccgaac gggtagcatc
     1321 gccatagtcc tgcgggaagg gcggtattgc cttgtccgag tctgccggat tgacgccgca
     1381 ggagcaggcg ttgatgcctg cgtccagttc ctctgccagt tccggataag cccggcgcca
     1441 ttgttcgtac atggcgttcc aggcattgaa atctttttcc cgttgggctt tcaggtctgc
     1501 gaaagcggta cggacgtctt cggagacgta ataacgttct ccggcaggaa tttcccagtt
     1561 ggcgtgcgct tcttcctgaa gcttggcccc gccttcccca tggccttttg tggtgccttc
     1621 aatgccggga atgcccttgc cgatgaccgt tttggcaatg atgagctggg gtttcccgtt
     1681 tttggcgttt ttggcggctt caaccgctga cttaatggcc ttgatgtcgt gcccgtcaat
     1741 ctggcgcaca tcccagccca gggcttcgta tacggcgcgc ggatccgtaa gctgggtacg
     1801 ttccgcggga gcgtccagcg tgatgtcgtt ggaatcgtaa atgagaataa ggttatccag
     1861 cttgagcacg gcggccaggg cgagggattc ccgggcgacg ccttcctgca ggcagccgtc
     1921 tcccgtgagg cagaagacgt gctgggtgaa gatatccatg ccgggcctgt tgaaccgggc
     1981 agccgcgcgc ctggcggaga gggcgaagcc tacggcgttc gcaataccct ggcccagcgg
     2041 tcccgtggtg cattctacgc cttccgtatc ccggaattcg gggtggccgg gcgtgatgga
     2101 acctttgtga cggaagtttt tgatatcttc aatgccgatg ttgaatcctg acaggtggag
     2161 ccagccgtaa agaaacatgg agccgtggcc ggcggagaga atgaagcggt cacggttgag
     2221 ccagcggggc tgtgaagggc agatgttgag caaatcgccg aacaggacgg ccccgatttc
     2281 ggcgcacccc agcggcaggc ccaggtggcc ggatgcacag tcgtgaacgg catccatggc
     2341 gagtccccgc gcctggttgg cggctttttg gagaaggtca agattcatgc ccgggcaata
     2401 tgagcggatc agcttcatat tggaagccta aaatgcgtct ccggcgccag ttccggaccg
     2461 gggaaaagca ttgaagatgg aagcgccgcg catgccgctc ccggttttgc ggcacgctca
     2521 ccggaatgta tggcgcttta gggaataaag ccgggggaaa catgtcggac ggcatgctcc
     2581 agtccttttc tccggtgttg tccgcccctg gttttcatgg atgtccagat attggccggg
     2641 atggcggatt tcatccgcgc cggagccggt cagtcggagg gaatg
//
LOCUS       DAOMSU010000292         2669 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3951_length_2669_cov_2.963657, whole genome shotgun sequence.
ACCESSION   DAOMSU010000292 DAOMSU010000000
VERSION     DAOMSU010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2669
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3951_length_2669_cov_2.963657"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PK077_09720"
     CDS             complement(<1..373)
                     /locus_tag="PK077_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24615.1"
                     /translation="MSPTGKFTLRLVIYGAFLLYLVGDLFIWHGFLAGRMDAYLKPLP
                     GPPGDNSARIAEVYGEPVTANQLSRRITELKMLRQPPVLDMEGGIKLTHEPDIPGDLA
                     PRARYDLIGSSLLRLKTSVNDL"
     gene            complement(398..1492)
                     /locus_tag="PK077_09725"
     CDS             complement(398..1492)
                     /locus_tag="PK077_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRN24616.1"
                     /translation="MAVIDQYFKYLVEAGGSDLHLSEGQPPKVRVNGTISAISDENLE
                     GQAFKNLLAEICDPQAFQKYLEEGDLDFAYEMDETSRFRCNYFKQQHGLGAVFRLIPT
                     KIATLEELGVPTVVKEFAHMRSGLVLVTGPTGSGKSTTLAAIIDYINSNQARHIITVE
                     EPIEFVHPNKKSIVTQREVPLQTPSFADGLRASLREDSDIVLVGEMRDLETISLALTA
                     AETGLLVFGTLHTNNASKTIDRIIDVFPSDQQSQVRTMLAGSLRGVVAQLLMKRSDKP
                     GRVAVNEILFATPAVAAIIREGATQKIPDVIVGGKAMGMQFMDDAIWQKLQQGIVSPE
                     EAYMKCIEKKRFRNFLPPESARLADSGGGN"
     gene            complement(1518..2600)
                     /locus_tag="PK077_09730"
     CDS             complement(1518..2600)
                     /locus_tag="PK077_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018968777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HRN24617.1"
                     /translation="MSEYILPHVDGYLKLGQDYDCSDIHLAVNSRPTWRRFGQLLPIW
                     EEAPNLTPQDTEVLARGFLEDPEWNRLQQRGDVDFAYANDFGRYRASVVKQRLGYDIC
                     FRIINTRVRTMEEIGLPTEYVVPLTRYTNGLILVTGSVGSGKSTTLASIVDFINQDRH
                     DHIITLEDPIEYLIPSKNCHVNQREVHKHTESFARALRGALREDPDVIMVGEMRDLET
                     ISLALTAAETGHLVLGTLHTGSAARTVDRVLDVFPVEQRDQIRIMVSESLRGIISQQL
                     VPRADGNGRALAIEMLVNTPAIANCIREGKTFMIPGCIQTGKAQGMILMDDSLRALHQ
                     AGLITTEDCIFRAEDKTIMKSFLGIK"
BASE COUNT          532 a          679 c          857 g          601 t
ORIGIN      
        1 ggaggtcatt gacgctggtt ttcaggcgca gaagggagga gccgatcagg tcatacctgg
       61 cccgcggagc caggtcgccg gggatgtccg gttcatgagt aagtttgatc ccgccttcca
      121 tatccagcac gggaggctgg cgcagcattt tcagttccgt aattcttcgg gaaagctggt
      181 tggcggtgac gggttccccg tacacttccg ctatccgggc ggagttgtct cctggcggcc
      241 ccggcaacgg tttcaggtac gcgtccatcc tgccggccag aaagccgtgc cagataaaca
      301 agtctcccac cagatagagc aggaaggctc cataaatcac aagcctgagc gtgaattttc
      361 cagtgggaga catggcagga gaaggagggc aaagagctta gtttccgccg ccggagtctg
      421 ccaggcgggc ggattccgga ggaaggaagt tgcggaatct cttcttttca atgcacttca
      481 tgtaggcttc ttccggggag acgatgccct gttggagttt ttgccagatg gcgtcatcca
      541 tgaattgcat gcccatggcc ttgccgccga cgatgacgtc agggattttc tgcgtggcgc
      601 cttcacggat gatggccgcc acggccggcg tggcaaagag aatttcgttg acggctacgc
      661 gtcctggttt gtcgctgcgc ttcatcagga gctgcgccac gacgccgcgg agggaacccg
      721 caagcatggt gcgcacctgg gattgctggt cagacgggaa tacgtcaatg atacggtcaa
      781 tggttttgct ggcgttgttc gtgtgcaggg tgccgaatac cagaaggccg gtttccgccg
      841 ccgtgagcgc cagggagatg gtttccaggt cgcgcatttc acccaccagc acgatgtcgg
      901 agtcttcacg aagggaagcc cggaggccgt ctgcgaagga aggcgtctgg agaggcactt
      961 ccctctgggt gacgatggat tttttattgg gatggacgaa ttcgataggt tcctccacgg
     1021 tgatgatgtg ccgggcctgg ttgctgttga tatagtcgat gatagcggcc agcgtggtgg
     1081 atttaccgga gccggtgggc cccgtgacca gcacaaggcc ggatctcata tgggcgaatt
     1141 ccttgaccac cgtaggcacg cccagttctt ccagcgtggc tattttggtg ggaatgaggc
     1201 ggaaaacagc ccccaggccg tgctgctgct tgaagtagtt gcagcggaaa cgggatgttt
     1261 catccatttc ataggcaaag tccaggtccc cttcttccag atatttctgg aaggcctggg
     1321 gatcgcatat ttccgcgaga aggtttttga acgcctggcc ttccaggttt tcgtcggaga
     1381 tggcggagat ggtcccgtta acgcggactt taggaggctg gccttcgctg aggtgaaggt
     1441 cggaaccgcc cgcttctacg aggtatttga agtattggtc gatgacagcc ataacaggag
     1501 tgtttcaaaa ttaaaggtta cttgattccc aggaaggatt tcatgatggt tttgtcttcc
     1561 gcgcggaaga tgcagtcttc cgtggtgatg aggcccgcct ggtgcagagc ccggagcgag
     1621 tcgtccatca ggatcatgcc ctgggctttc ccggtttgaa tgcagccggg aatcatgaat
     1681 gtcttgcctt cgcggatgca gttggcgatg gcgggcgtat taaccagcat ttcaatggcc
     1741 agagcgcggc cgttgccgtc cgctctggga accagctgct gggagatgat gccgcgcagg
     1801 gattcgctga ccatgatgcg gatctggtcg cgttgctcca ccgggaagac gtccagcacg
     1861 cggtccaccg tacgggcagc ggaacccgtg tgcagggtac cgagcaccag gtggccggtt
     1921 tcagccgccg tgagcgccag ggagatggtt tccaggtcgc gcatttcccc caccatgatg
     1981 acgtcagggt cttcacggag ggcgccgcgg agcgcccggg cgaaggattc cgtgtgttta
     2041 tgcacttcac gctggttgac gtggcaattt ttggagggaa tcaggtattc gatggggtct
     2101 tcaagcgtga tgatgtggtc gtggcggtcc tggttgatga aatccacgat agaggccagc
     2161 gtggtggatt taccggaacc tacggaaccc gtgactaaaa tgaggccgtt ggtgtagcgg
     2221 gtgaggggca ccacgtattc cgtcggcagg cctatttcct ccatggtgcg cacccgcgtg
     2281 ttgatgatgc ggaagcagat gtcataaccc aggcgctgtt taaccacgga tgcgcggtaa
     2341 cggccaaagt cattggcgta ggcgaagtcc acgtcccccc gttgctgaag gcggttccat
     2401 tccgggtctt ccagaaagcc cctcgccagc acttccgtgt cctggggcgt gagattgggc
     2461 gcttcctccc agatggggag aagctggccg aaccggcgcc aggtgggacg gctgttgacg
     2521 gcaaggtgga tatcggaaca gtcataatcc tgtccaagtt tgagatatcc gtcgacgtgg
     2581 ggcaaaatgt attcgctcat gttgtgaatg cagcaaactg ataaagctac atattaacga
     2641 aaacagagct tttttgacca gcaaaaaat
//
LOCUS       DAOMSU010000293         2667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_3960_length_2667_cov_2.319296, whole genome shotgun sequence.
ACCESSION   DAOMSU010000293 DAOMSU010000000
VERSION     DAOMSU010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3960_length_2667_cov_2.319296"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..2402)
                     /gene="katE"
                     /locus_tag="PK077_09735"
     CDS             complement(135..2402)
                     /gene="katE"
                     /locus_tag="PK077_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HRN24618.1"
                     /translation="MNKPMKKKKPPMEDSAAPFPNGKEAAPHYTDTIDPEIIKPTPKP
                     TPPNAEPSAPGSMKMPDNATEKIRELDAMRSNGMGQALTSNLGVKISDDQNTLKAGSR
                     GPSLLEDFHFLEKMAHFDQERIPERVVHARGSGAHGYFQVYKSLSKYTKAAFLQDPGE
                     KTPVFVRFSTVQGFRGSPDTVRDIRGWATKFYTKEGNYDLVGNNTPVFFIQDAIKFPD
                     FVHAVKPEPHNEMPQGQTAHDSFWDYVSLQPETLHNVMWAMSDRGIPRSFRTMEGFGI
                     HTYKLVNEEGKSTFVRFHWKPVYGKKSLVWDEAQVLTGRDPDFHRKDLWQSIEAGDYP
                     EYELGLQLIPEEDADQFDFDILDATKLIPEALVPVEIVGKMVLNRNPDNFFAETEQAA
                     FCPANIVPGIDFSDDPLLQGRIFSYSDTQRHRLGGANFTEIPINRPICPFHNNQRDGF
                     HRMQIDASPANYDPNSIGNNWPRETPPEKGGFTTSPQTVSGVKERLRSPSFAEYYSHP
                     RLFWMSQTPVEQEHIINAFSFELGKVTRPYIRERVVDLLTRIDPDLASGVARNLGIQL
                     TREQLSRELPRPVCGLEQDPSLSLYAHTDGNLKGLRVSLLAADGVSLKSVKEICEALH
                     EEGIHPQIIAPHMGSVTTEEGESLPVNGTLSGTPSVLFDSVIVPEGEQSIAALLKDGD
                     AKYHLRQAYRHLKAIGLPGNARAMLEAASLPQDMDDAGLLMPKDTKSLMPSFITAMKQ
                     HRVWSREPKTLDFGA"
BASE COUNT          520 a          651 c          783 g          713 t
ORIGIN      
        1 gaatgtttct acttcccttt ttcatttttc ctgaagtatt gtttaacatt ccgggtttgc
       61 tgataaaaaa cggctgccgc gccttttggc ggggcagcct gtattgggaa gcggttcctg
      121 aataaggaac gaatctaggc gccgaaatca agggttttag gctcgcggct ccagacgcgg
      181 tgctgtttca tggccgtgat gaaggagggc atcagggatt tggtgtcctt cggcatgagc
      241 agtccggcat catccatatc ctggggcagg gaggctgcct caagcatggc tctggcgttg
      301 ccgggcagtc cgatggcttt caggtgcctg taggcctggc gcaaatggta cttggcatct
      361 ccgtctttca ggagcgctgc gatgctttgt tctccttccg ggacgatgac ggagtcaaac
      421 aggacggaag gagtgccgga cagagtcccg ttaaccggca gactttcccc ctcttccgtt
      481 gttacgcttc ccatgtgcgg ggcaatgatt tgggggtgga tgccttcttc atgcagggcc
      541 tcgcagattt ctttcacgga tttcaggctg acgccgtccg ctgccagtaa agagacgcgg
      601 aggcctttga ggttgccgtc cgtatgggca tacaggctca atgacggatc ttgttccagg
      661 ccgcagacgg gcctgggaag ttccctgctg agctgttccc tggtgagttg aattcccagg
      721 ttgcgggcca ctccgcttgc caggtccgga tcaatgcgtg tcagaaggtc caccacgcgt
      781 tcccggatat aggggcgcgt caccttgccc agctcaaagc tgaacgcgtt gataatgtgc
      841 tcctgttcca ccggagtttg actcatccag aacaaccggg ggtgggaata gtattccgcg
      901 aaggaggggc tccgcagacg ttccttgaca ccgcttaccg tctgggggct ggtggtgaag
      961 ccgcctttct ccgggggcgt ttctctcggc cagttgttcc cgatggaatt gggatcatag
     1021 ttggccggag aggcgtctat ctgcatacgg tgaaagccgt ccctctggtt gttgtggaag
     1081 gggcaaatgg ggcggttgat gggaatttcc gtgaaattgg ctcctcccag ccggtgccgc
     1141 tgggtatcgc tgtaagagaa aatacggccc tggagcagcg gatcgtctga aaaatcaatg
     1201 cccggcacga tattggcggg gcagaaggct gcctgttccg tttccgcaaa aaagttgtcc
     1261 gggttgcggt tcagcaccat tttcccgacg atttccacgg gaaccagtgc ttccggaatg
     1321 agcttggtag cgtccagaat atcgaagtcg aactggtctg cgtcttcttc cgggatgagt
     1381 tgcagcccca gctcgtattc cggataatct ccggcttcaa tggattgcca gagatccttg
     1441 cggtggaagt caggatcgcg tcctgtcaat acctgggctt catcccacac cagggatttt
     1501 ttcccgtaca ccggtttcca gtggaaacgg acgaaagtgc tttttccttc ttcattgacc
     1561 agtttgtatg tatggatgcc gaacccttcc atcgtacgga agcttctggg gatgccgcgg
     1621 tccgacatgg cccacatgac gttatgcaga gtttccggct gcagggagac gtagtcccag
     1681 aaagagtcat gggccgtctg cccctggggc atttcattgt ggggttccgg tttgacggca
     1741 tgaacgaaat ccggaaattt gatggcatcc tgaatgaaga agacgggcgt gttattgcct
     1801 accagatcat agttgccttc cttggtatag aatttggtag cccaaccgcg gatatccctt
     1861 actgtatccg gagatcctct gaagccctgc acggtggaaa aacgcacaaa gaccggggtt
     1921 ttttctcccg gatcctgcag aaaagccgct ttggtgtact tggaaaggga tttatacacc
     1981 tgaaaataac catgtgcccc ggaacctctt gcgtgaacca cgcgttccgg tatccgctcc
     2041 tggtcaaaat gagccatttt ctccagaaag tggaagtctt caagcagaga ggggcctctg
     2101 cttcctgctt taagtgtgtt ttgatcatcg gatattttaa ctcccaggtt gcttgtgagg
     2161 gcctgtccca tgccgttgga gcgcatggca tccagctccc tgattttttc cgtagcgtta
     2221 tccggcatct tcatggaacc gggcgcggaa ggttccgcat tgggcggcgt aggtttcgga
     2281 gtgggtttga ttatctccgg atcaatcgta tccgtataat ggggagcggc ttcttttccg
     2341 ttgggaaaag gggcggcgct gtcttccatg ggcggtttct tttttttcat tggcttgttc
     2401 atgagggacc ggatttttct atgtgggtaa tagttcgact tccctttttc cggggatgtt
     2461 ccgtcctgtt ttgtcatcag tatttctccg gatctgatga aaaatttttt atagaaactg
     2521 gtatttaata ttttatggga taaagaacgg gctgtgtgga gagggctttt tgattggcat
     2581 gtctgtgatc tctgtatccc atgtttcatt gtcggcaggg agaagcggag ataaccgggg
     2641 tacggccgcg ggctggcaga aaacagt
//
LOCUS       DAOMSU010000294         2637 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4019_length_2637_cov_3.143687, whole genome shotgun sequence.
ACCESSION   DAOMSU010000294 DAOMSU010000000
VERSION     DAOMSU010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_2637_cov_3.143687"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /gene="folP"
                     /locus_tag="PK077_09740"
     CDS             <1..718
                     /gene="folP"
                     /locus_tag="PK077_09740"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRN24619.1"
                     /translation="VSHAKELERQGALILDIGGESTRPGAAEVAAEEELDRVLPVVRE
                     LRSCTDAVISVDTRHAAVAAAVLEAGADVINDISGLREAGMAELCAAARCGVVAMHMQ
                     GRPETMQDAPSYGDVVREVRGYFEERYDFLLGRGLEPEQICWDPGIGFGKTAEHNLAL
                     LSNMDKLQVAGRPVLLGLSRKRMLGAVLGDAEQGRAPLSTAVMTVWGHLHGAQIHRVH
                     DVAECARALRLIRAAEPFVR"
     gene            726..>2637
                     /locus_tag="PK077_09745"
     CDS             726..>2637
                     /locus_tag="PK077_09745"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HRN24620.1"
                     /translation="MESASSRPVLTSLASIAFSLAVVFLFLAVRGIWPFGGRFLEYMD
                     NGQLVYPTLKYYASALMSGAFDGAFFYDVNGGAGIRVSPTLPHQLLIPSTWIAVAMGD
                     SFLLKDMVWVMLADVACICVTASWFLRRVFPSLPVCWTVLLTAGYAVGGFFQTKYGFM
                     QFLDHAAMFPLFALGLYRLVNGGRGWLYAAGLFLLATSLYSAFMAVVTGWLFAWAFTL
                     PLKGTRERRVRLARVFWYTAAVTLATCYYWLPMAEMSRDSMRSLFMTPPTFFELSWPF
                     DPPKFLERLYACLPGMSFAALAAVYLACGKKASPAPDRGRRLFILLLAASVLPAFVEP
                     IHRAAHLWSYVDFPVRFGFIPNLAVMSFCAWILSGGRLPGPAGRPWGWGWSLGLPIAA
                     FFFSLFVLKMTAADLAVRLLPLLFFACAWLCWRHVEGKRLAWSIASVMMLGLPIGAAA
                     FWKQGEEEKASVQALNAEWLARGMKGYSGLLRVKDRDRLMVENADCLGPVPGIGNFRH
                     TTSIRHFRFLKNLGYRDEFTRTYGQGGTLFSDLLLGHGFVLASRPMEGMECVLFREGM
                     YLYRLPGARWGLVVSQGALGLRLDENADVFSNLNALHAALCPSAEGPLYVPVTMRTEM
                     SGPFYRADIPEYP"
BASE COUNT          382 a          720 c          905 g          630 t
ORIGIN      
        1 tgtctcccat gcaaaagagc ttgaacggca gggagccctt attctggaca taggggggga
       61 gtccaccagg ccgggggccg cggaggttgc cgcggaggag gagttggaca gggtgctgcc
      121 tgtggtgcgg gaactccgtt catgcacgga tgccgtgatt tccgtggaca cgcgccatgc
      181 cgccgtagcg gcggctgtgc tggaagcggg tgcggacgtc attaatgata tttccggatt
      241 gcgggaggcg gggatggcgg aattgtgcgc tgcggcgcgc tgcggcgtgg tggcgatgca
      301 catgcaggga cggcctgaaa ccatgcagga cgctccttct tatggggatg tggtgcggga
      361 agtgcgcggc tattttgagg aacgttatga ttttctgctg gggcgggggc tggaaccgga
      421 gcagatctgc tgggatcccg gcatcggctt tggcaaaacg gcggaacaca atctggccct
      481 gctgtccaac atggacaagc ttcaggtggc aggacgcccg gtgctgctgg gcctttcccg
      541 caagcgcatg ctgggcgccg tcctggggga tgcggagcag ggacgcgcgc cgctgagcac
      601 cgccgtgatg acggtgtggg ggcatttgca cggcgcgcag attcaccgtg tccatgacgt
      661 ggcggaatgc gcccgcgccc tccggctgat ccgggccgct gaaccgtttg tccgctgatt
      721 gagccatgga atccgcttct tcccgtccgg tattgacgtc cctggcgtcc attgcgtttt
      781 ccctggcggt ggtgttcctt tttctggcgg tacgggggat ctggcctttc ggaggccgct
      841 tcctggagta tatggataac gggcagctgg tttatcctac cttgaagtat tatgcctccg
      901 ccctgatgtc cggagcgttt gacggagctt ttttttatga tgtcaatggc ggcgcgggaa
      961 tccgggtgag ccctacgctg ccgcatcagc tgctgatccc ctccacctgg attgccgtgg
     1021 cgatggggga ttccttcctg ttgaaggaca tggtctgggt gatgctggcg gatgtggcct
     1081 gcatctgcgt gacggcttcc tggttcctga gacgcgtgtt tccctccctg cccgtatgct
     1141 ggaccgtgct gctgacggcg ggttatgccg tggggggctt tttccagacc aagtacgggt
     1201 tcatgcagtt cctggaccat gccgccatgt tccccctgtt tgccctgggc ctttaccggc
     1261 tggtgaacgg gggaaggggc tggctgtatg ccgccggcct cttcctgctg gctacctccc
     1321 tgtacagcgc gttcatggcc gtcgtcaccg gctggctgtt cgcgtgggcc tttacgctgc
     1381 ccctgaaggg gacgcgggaa cgccgcgtgc gcctggcccg cgtgttctgg tacacggcgg
     1441 cggtgacgct ggccacttgt tattactggc tgccgatggc ggaaatgagc cgggattcca
     1501 tgcgctccct gtttatgacg ccgcccacgt tttttgagtt gtcatggccg tttgaccctc
     1561 ccaaattcct ggaacgcctt tacgcctgcc tgccggggat gtcatttgcc gctctggcgg
     1621 cggtttattt ggcttgcggg aagaaggcgt ccccggctcc ggacagaggg aggcgcctgt
     1681 ttatccttct tctggcggcg tccgttttgc ccgcgtttgt cgagcccatc caccgcgccg
     1741 cccatttatg gagctacgtg gattttcccg tccggttcgg ttttattccc aatctggcgg
     1801 taatgtcgtt ctgcgcctgg attttgtccg gcggcaggct tccggggccg gcaggacggc
     1861 catgggggtg gggatggagc ctggggcttc ccatagcggc ttttttcttt tccctgtttg
     1921 tcctgaagat gacggctgcg gatcttgcgg tgcgtcttct gcctttgctg ttttttgcat
     1981 gcgcatggct gtgctggagg catgtggagg gcaaaaggct ggcgtggtcc atcgcttccg
     2041 tgatgatgct ggggctgccc ataggggctg ccgcgttctg gaagcagggg gaggaggaga
     2101 aagcctccgt ccaggcgctc aatgcggaat ggctggcgcg cggcatgaag gggtattcag
     2161 gcttgctgcg cgtgaaggac agggatcgcc tgatggtgga aaacgcggat tgcctggggc
     2221 ctgtcccggg cattggcaat ttccggcata cgacgagcat caggcatttc cgttttctga
     2281 aaaacctggg gtaccgggat gaattcacgc gcacatacgg ccagggaggc actctgtttt
     2341 ccgatttgct gctgggccat ggttttgtgc tggcgtcccg acccatggag ggaatggagt
     2401 gcgtgctttt ccgggaaggc atgtatttat acaggcttcc gggcgcccgc tgggggctgg
     2461 tggtttccca gggcgcgctg gggttgcgtc tggatgaaaa cgctgatgtg ttcagcaatc
     2521 ttaatgccct gcatgccgcg ctctgcccat cagcggaagg ccccctttac gtacccgtaa
     2581 caatgaggac ggagatgagc ggcccctttt accgcgcgga cattccggaa tatcccg
//
LOCUS       DAOMSU010000295         2636 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4027_length_2636_cov_3.016660, whole genome shotgun sequence.
ACCESSION   DAOMSU010000295 DAOMSU010000000
VERSION     DAOMSU010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4027_length_2636_cov_3.016660"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..710)
                     /locus_tag="PK077_09750"
     CDS             complement(36..710)
                     /locus_tag="PK077_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HRN24621.1"
                     /translation="MTSPGNHILPHYDAALNAIRLRVNAICGSLLKYMDILEQVISGP
                     DSDGANSIIADSDPLQEETQQVLSLCSSVLTQFHPLGRDLRLVITFSRCGDKLQECME
                     EVTGIAKHAKASIRHQESLSPDIVLPLLEMAVSEFRDAARCLETQDVDAARGIRLRDK
                     KLDKAHRKALALLVCPERAGAPALNVNLLFIIRSLERIGDIAKTIAATVVFLKEATDI
                     RHGKEK"
     gene            complement(736..1515)
                     /gene="pstB"
                     /locus_tag="PK077_09755"
     CDS             complement(736..1515)
                     /gene="pstB"
                     /locus_tag="PK077_09755"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRN24622.1"
                     /translation="MTEPAAADDEPIIEVEDFSFFYGSKQVLFNIGMTFETNRVTALI
                     GPSGCGKSTLLRNINRMNDLVPDVRHQGDIRIDGTSLYDPRVEVISLRKRVGMVFQKY
                     NPFPKSIYENIVFPLRVAGRNRRAELDETVERSLKGAALWDEVKDKLHESAYGLSGGQ
                     QQRLCIARAIANRPQILLMDEPCAALDPIATLKIEDLMEDLKKELTIVIVTHNMQQAT
                     RIADRTAFMYMGRLVEYGETSQIFTNPTEKETEAYITGRFS"
     gene            complement(1538..>2636)
                     /gene="pstA"
                     /locus_tag="PK077_09760"
     CDS             complement(1538..>2636)
                     /gene="pstA"
                     /locus_tag="PK077_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRN24623.1"
                     /translation="RTAVPRKNPILTDMDDPAGELDRLRAKVEQLNAEYARYTAEAAK
                     LRAQQGRDSLVYALGDGEKKEIRMDKIVYGYQPNDLGFSGKCGVFLHSLYHFVMDDPR
                     EANTEGGIFPAIFGTFIMTLLMSVLVTPVGVIGAIYLREYARQGHLVQAVRICVNNLA
                     GVPSIVFGVFGLGFFVYYLGGSIDELFYWKKLAVDNTPTFGTSGILWASLTLALMTLP
                     VVIVSTEEALSAVPRGLREAALACGASKWQMIKRIILPSALPGVMTGLILAMARGAGE
                     VAPLMVTGVVKLAPSLPIDGEGPFIHLERKFMHLGFHIYDVGFQSPDSDAAQPMVFAT
                     TLLLILLVVVMNLTAILIRNRLRKKYAASSF"
BASE COUNT          492 a          687 c          879 g          578 t
ORIGIN      
        1 ggcaacaggg aatcgccgtg cgggcaggaa ttgttttatt tttcttttcc gtggcggatg
       61 tccgtggctt ccttcaggaa gacgacggtg gcggcgatgg ttttggcaat gtctccgata
      121 cgttccagag aacggataat gaagagaaga ttgacgttga gggcaggggc tcccgcccgt
      181 tcggggcaga ccagaagggc cagcgctttg cgatgggctt tgtccagttt tttgtcgcgc
      241 aggcgtattc ccctggcggc gtccacatcc tgcgtttcca gacaccgggc ggcgtctctg
      301 aattcggata cggccatttc cagcagaggg agcacaatat ccggggagag ggattcctga
      361 tgcctgatgg aggccttggc atgcttggcg atgcccgtta cttcctccat gcattcctgg
      421 agcttgtctc cgcaccggga gaaggtgatg acgagcctca ggtcgcgccc cagcggatgg
      481 aactgggtga gtacggagga gcagagggag agaacctgct gggtttcttc ctgcagcggg
      541 tcgctgtccg caatgatgct gttggccccg tcgctgtccg ggccggaaat gacctgttcc
      601 agaatgtcca tgtatttcag caggctgccg cagatggcgt tgacgcggag gcggatggcg
      661 ttcagggccg cgtcgtagtg gggaaggatg tgattgccgg gagatgtcat gaggagaatg
      721 gtttggaaag ggcttttagc tgaaacggcc cgtaatgtag gcttccgttt ctttttccgt
      781 gggattggtg aagatttggg acgtttcccc gtattccacc aggcggccca tgtacatgaa
      841 ggcggtccgg tccgcgatgc gtgtggcctg ctgcatgtta tgggtgacga tgacaatggt
      901 gagttctttt ttcaggtctt ccatcaggtc ctctattttt aaggtggcga tagggtccag
      961 ggcggcgcag ggttcatcca tcagcaggat ctgggggcgg ttggcaatgg cgcgcgcgat
     1021 gcacagccgc tgctgctggc ctccggaaag gccgtaggcg ctttcatgca gcttgtcttt
     1081 cacttcatcc cacagggcgg ctcccttcag gctgcgttcc actgtttcgt ccagttccgc
     1141 gcgcctgttg cggccggcca cccggagggg aaagacgatg ttttcgtaaa tgcttttagg
     1201 gaaagggttg tatttctgga agaccatgcc tacgcgcttg cgcagggaga tgacttccac
     1261 gcgcgggtca tacaggctgg tgccgtcaat gcggatatcc ccctggtggc gcacgtccgg
     1321 gaccaggtcg ttcatgcggt tgatgttgcg gagcagagtg gatttcccgc agccggaggg
     1381 cccgatgagg gcggttaccc ggttggtttc aaaggtcatg ccgatgttga agagaacctg
     1441 tttgctcccg taaaagaagg agaagtcttc cacttcaatg atgggctcgt cgtctgcggc
     1501 ggctggttca gtcatgggat gatgaaggag aatggggtta gaagctggag gccgcgtatt
     1561 ttttgcgcag gcggttgcgg atgaggatgg ccgtgagatt catgaccacc accagcagga
     1621 tgaggagcaa cgtggtggcg aaaaccatgg gctgggcggc gtccgagtcc ggagactgga
     1681 agcccacgtc gtagatatga aagcccagat gcatgaattt gcgttccagg tggatgaagg
     1741 ggccttcccc gtcgatgggc agggaggggg ccagcttgac cacccccgtg accatgagcg
     1801 gggctacctc tccggcgccc cgggccatgg ccagaatgag gccggtcatg acgcccggca
     1861 gggcgctggg gaggatgatg cgtttaatca tctgccattt ggaggccccg caggccaggg
     1921 ccgcctccct cagcccgcgg gggacggcgg agagtgcctc ctccgtggag acgatgacta
     1981 cgggcagcgt catcagggcc agcgtgaggg aagcccacag gattccggag gtcccgaagg
     2041 tgggcgtgtt gtccacagcc agctttttcc agtagaaaag ttcgtcgatg ctccctccca
     2101 gatagtaaac gaagaagccg aggccgaaaa cgccgaagac gatggaaggc acgcccgcca
     2161 ggttgttcac gcagatgcga acggcctgta ccagatgccc ctgcctggcg tattccctca
     2221 ggtagatggc gccgatgacc cctacgggcg taaccagcac gctcatgagc aaagtcatga
     2281 tgaaagtgcc gaagatggcg gggaagatgc cgccctccgt attggcctcc cgcggatcat
     2341 ccatgacgaa atgatacagg ctgtggagga agacgccgca tttgccggag aatcccaggt
     2401 cattcggctg gtagccgtaa acgattttat ccatgcggat ttctttcttt tccccgtctc
     2461 ccagggcata cacaagagag tctcggccct gttgagccct cagtttggct gcttccgcgg
     2521 tgtaccgggc gtattcggcg ttgagctgtt ccactttggc gcggagccgg tccagttccc
     2581 cggccgggtc gtccatatcc gtgagaatgg ggtttttcct ggggacggcc gtacgc
//
LOCUS       DAOMSU010000296         2630 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4050_length_2630_cov_2.608544, whole genome shotgun sequence.
ACCESSION   DAOMSU010000296 DAOMSU010000000
VERSION     DAOMSU010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4050_length_2630_cov_2.608544"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1378)
                     /locus_tag="PK077_09765"
     CDS             complement(<1..1378)
                     /locus_tag="PK077_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HRN24624.1"
                     /translation="MSSFFIKHPTIAIVISIVMILLGGLSLMGLPIEQYPNIVPPTIK
                     MQATYPGANAETVANSVASPIEQSISGVVGMDYMTSTNANNGICSLSIVFEVGTDPNM
                     DQTLAYMRYGQATAQIPAEVSQMGITITQSTGSPLAVINLYSPDDSLNAIFLSNYAYV
                     SLVDPVKRVPGVGDVQVFGAGRYAMRIWLDTTKMAAQNISVGEVQSAIQAQNTVIPGG
                     QIGAEPAPPGTEFTYSIQTKGRLQTAEEFGNIIIRADGNKLLYLKDIAKVELGSQTYN
                     VSSKYNGRDSGAIAVYAAPGSNAINTVDALVKLFEDRSRSFPAGMEYNLTLDTTLAVR
                     ASIEEIEHTLVEALLLVVLVVFVFLQGWRATLIPAIAVPVSIIATFALFPLLGFTLNT
                     ICLMGMVLAIGLVVDDAIVVVEAVESHMERGLTPRQAAFAAMEEVSGPVIAIALVLAA
                     VFLPSLLLP"
     assembly_gap    1441..1463
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1464..>2630)
                     /locus_tag="PK077_09770"
     CDS             complement(<1464..>2630)
                     /locus_tag="PK077_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRN24625.1"
                     /translation="KDSAAQAPALVPEVQVTKLVTKDVPIRQEWVGTLRGTEDAEIRS
                     QVTGYLLSKDYRDGAYVKKGQVLFQIDPRPFQATLDQAQGRLEQYEATLKKYKLDVER
                     YTPLVKTGSVSRKQLDDALQQVQETEAAIATAKAQVDEAKINLKFTTITAPISGLAGL
                     ATPSIGNLLTPSSPTPLTTISSIDPIRVDFSVSERDFLNSMDNNLTRQKHLKFDVILA
                     NGTEYPMQGEPVAIDRNVDAQTGTINIVGHIPNPNLTLRPGMFVRVRATVKTLENAAL
                     VPPRAILSAQSAKFIIYLDDKNIPHMQVVNLGPVVDGMQVINIIPMPHSTFTKDSPIV
                     VEGIMQAAKVQGQAPVKPLPYKPVVSQPVMPSIGAQSFEKARTPEGMKDGEAQP"
BASE COUNT          487 a          699 c          871 g          550 t
ORIGIN      
        1 ccggcagcag cagggaaggc aggaacaccg ccgccagcac cagggcgatg gcgatgacgg
       61 gaccggatac ttcctccatg gcggcgaaag ccgcctggcg gggcgtgagg ccgcgctcca
      121 tgtgggattc cacggcttcc accaccacga tggcgtcgtc caccaccagg ccgatggcca
      181 gcaccatgcc catgaggcag atggtgttca acgtgaagcc caggagagga aacagcgcga
      241 aggtggcgat gatggaaacc ggaacggcga tggccggaat cagcgtggcg cgccagcctt
      301 gcaggaagac gaacaccacc agaaccacca gcaggagggc ttccaccagc gtgtgctcaa
      361 tttcctcaat ggaggcgcgc acggccagcg tggtgtccag cgtcaggttg tattccattc
      421 cggcggggaa gctgcgggag cggtcttcaa agagcttgac cagggcgtcc accgtgttga
      481 tggcgttgga gccgggagcg gcgtacacgg cgatagcgcc ggaatccctg ccgttgtatt
      541 tgctggatac gttgtaagtc tgggagccca gctccacctt ggcgatgtct ttcaggtaaa
      601 gaagcttgtt cccgtccgca cggatgatga tgttgccgaa ttcctcagcc gtctgaaggc
      661 gtcccttggt ctggatgctg tatgtgaatt ccgttccggg aggggccggc tccgcgccga
      721 tctggccgcc gggaatcacg gtgttctgcg cctggatggc ggactggact tctcccacgg
      781 agatgttctg ggcggccatt ttggtggtgt ccagccagat gcgcatggcg tagcggcctg
      841 cgccgaacac ctgcacgtcg cccacgccgg gaacgcgttt cacggggtcc accaggctca
      901 cgtaggcata gttgctcagg aaaatggcgt tcaggctgtc atccggagaa tacaggttga
      961 ttacggccag cgggctgcct gtggactgcg tgatggtgat gcccatctgg gagacctccg
     1021 cgggaatctg ggcggtggcc tgcccgtagc gcatgtaggc cagagtctgg tccatgttgg
     1081 ggtccgtgcc aacttcaaag acgatgctca gggagcaaat gccgttgttg gcgttggtgg
     1141 aagtcatgta atccatcccc acgacgccgg aaatggactg ctcaatgggg gaggctacgg
     1201 aattggccac cgtctccgca ttcgcgcccg ggtaggtcgc ctgcatcttg atggtgggcg
     1261 ggacgatatt cggatactgc tcaatgggca gccccatcag ggacaggccg ccgagcagga
     1321 tcatcacaat cgagatgacg atggcaatgg tcgggtgttt gatgaagaag ctggacatgg
     1381 cgttaaatca tgaaaatgcg cgggaaaagg gaagatggtg ggtctccgga taagggaggt
     1441 nnnnnnnnnn nnnnnnnnnn nnnacggctg ggcttctcca tccttcatgc cttctggcgt
     1501 cctggccttt tcaaaggact gggcccctat ggagggcatg acgggctggg aaactacggg
     1561 cttgtaggga agcggcttga cgggggcttg tccctgcacc ttggccgcct gcatgattcc
     1621 ctccaccacg atggggctgt ccttcgtgaa ggtggagtgc ggcatgggaa tgatgttgat
     1681 tacctgcatg ccgtcaacaa cggggccgag gttgacgacc tgcatgtggg gaatgttctt
     1741 gtcatccagg taaatgatga atttggcgct ctgggcggac agaatggcgc gcggaggcac
     1801 cagcgcggcg ttttccagcg ttttcaccgt ggcgcggacg cggacgaaca tgccgggccg
     1861 cagcgtcagg ttcggattgg gaatatgccc gacgatgttg atggtaccgg tctgggcatc
     1921 cacattcctg tcaatggcca cgggttcgcc ctgcatggga tattccgtgc cgttggcaag
     1981 aatcacgtca aatttgaggt gcttctgcct ggtcagatta ttgtccatgg agttcaggaa
     2041 gtctcgttcg cttacggaga aatccacccg aatggggtca atggaggaaa tggtggtcag
     2101 cggcgtgggg ctggagggcg tcagcaggtt gccgatggaa ggggttgcca ggccggccag
     2161 gccggaaatg ggggctgtaa tggtggtgaa tttaagattg atttttgctt catccacctg
     2221 ggcctttgcc gtggcgatgg cggcttccgt ttcctggacc tgctgcaggg cgtcgtccag
     2281 ctgcttgcgg ctgacggagc cggttttcac cagcggggta tagcgttcca cgtccagctt
     2341 gtactttttc agcgtggctt catattgttc caatctcccc tgggcctggt ccagcgttgc
     2401 ctggaacggg cggggatcaa tctggaaaag cacctggccc ttcttgacat aagcgccgtc
     2461 ccggtaatcc ttggaaagaa ggtatcccgt cacctgggag cggatttcgg catcttccgt
     2521 gccgcgcagg gtgcccaccc attcctgcct gatgggaaca tccttcgtga ccagtttggt
     2581 cacctgcact tcgggaacga gcgccggcgc ctgggctgcg gaatcctttt
//
LOCUS       DAOMSU010000297         2623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4074_length_2623_cov_1.602804, whole genome shotgun sequence.
ACCESSION   DAOMSU010000297 DAOMSU010000000
VERSION     DAOMSU010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4074_length_2623_cov_1.602804"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>804)
                     /locus_tag="PK077_09775"
     CDS             complement(<1..>804)
                     /locus_tag="PK077_09775"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRN24626.1"
                     /translation="TLSGIKDQLWDDYEIIAVDDGSTDGTAELLDRLDNPRLTVLHQR
                     NKGVSCARNTAINAARGVYIAFLDGDDVWLPEHLSHAAHFFQAHPDAAWYTSSYAKSF
                     SPEQDKKNIHSGEHPVFRLGHYFPDGIRCIHTSSTIIRRDAIPEGGMFPEDLRYGEDL
                     VAFATIASLHPVIGINDSITVLYRQRRDSAVHSPMDLNTKVEQDAAIFRHFMAIPANR
                     ENARATMNYYRSMSLSFWINRIMSSRLNTWLPSIRERKAATGTMVRGGWA"
     assembly_gap    805..814
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1077..2270
                     /locus_tag="PK077_09780"
     CDS             1077..2270
                     /locus_tag="PK077_09780"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRN24627.1"
                     /translation="MKVLISGMASWHMDKTAASFEKLGILSGMWVSNKNRGLPSGKYK
                     RIWPYHLGMKPFYHVAPAYLEEEMRWRNLWLYDCWVRRQELPADVDVVQCPMGSCSPV
                     FDLAEASGRTILKVFEAMNGHPTTQRGYWQREADIHSPGFRVPVGEKVWSRMNREIHR
                     ADCVLCPSTYVMESMVANGVSPEKCLLNHFGSDTSLFFKREKEDIPSFPKFIITGNLT
                     VRKGHQYLFLAFQKLVEQYPDAELHVYGGIRPDFRPLWEKWKTCPHLYHHASVSQQEL
                     ARRLASCTAFILPSLEEGFARSLLEAMAVGLPIIATHESGATTLLDGRSARIIPAADA
                     EAIYRAMKELCDNPALVHRLGEQAYAAFGGSENTWEAYAGRLAEAYRERLERLRESAG
                     QRGSR"
     gene            complement(2267..>2623)
                     /locus_tag="PK077_09785"
     CDS             complement(2267..>2623)
                     /locus_tag="PK077_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24628.1"
                     /translation="CADRKMLLSLLFQESATFKPINAFIAYFDATGISHTAATTMLEE
                     DARSLKETLPAAILDDYRLLLASPIAPYYMDMLVNFKTPRKNTAFFLARISRVAAKYL
                     LLLSSLCSARKNRPLP"
BASE COUNT          588 a          638 c          785 g          602 t
ORIGIN      
        1 gcccagccgc ccctgaccat agtgccggtc gcggcttttc tctcacgaat ggagggcagc
       61 cacgtgttga gccggctgct catgatccgg ttaatccaga agctcaggga catgcttcgg
      121 taataattca tggttgcgcg tgcattttcc cggtttgcgg gaatggccat gaaatgcctg
      181 aaaatggcgg cgtcctgctc tactttcgta ttcaggtcca tgggggagtg tacggcggaa
      241 tcccgccttt gccggtacag gacggtgatg gagtcattga tgccgatgac ggggtggagg
      301 gaggcgatgg tcgcgaacgc gacaaggtct tctccatagc ggagatcctc cgggaacatg
      361 cccccttccg ggatggcgtc acggcggatt atcgtagatg atgtatgaat gcagcggatc
      421 ccgtcaggaa aataatgacc caggcgaaaa acaggatgtt cgccggagtg aatatttttt
      481 ttatcctgtt cgggggaaaa gcttttggca tagctggacg tgtaccatgc cgcgtcggga
      541 tgggcctgga aaaaatgggc ggcatggctt aaatgttcgg gcagccatac gtcgtcgccg
      601 tccagaaagg caatatagac gcccctggcc gcgttgatgg cggtattgcg cgcgcatgat
      661 acgcctttgt tgcgctgatg aagtacggtc agccgcggat tgtcgagcct gtccagcagt
      721 tctgccgtgc cgtccgtgga gccatcgtca acggcgatga tttcataatc atcccacaac
      781 tggtctttaa ttcccgaaag agtcnnnnnn nnnnggatat aatccgctaa attataaaca
      841 gggatgataa tactgaaaaa gggggggcgg gaaggcattg ctgtgttgcg gcattttcgc
      901 cggacgaggc ttgaagttaa actgcacgga atacagtata agcggtacta tagaaatttc
      961 ccattcgtgg tcaatggcaa agaaagggaa tatctgcaat tcccgcagga acgggaaaag
     1021 ttcttcttga atatggtctc caattccttt agggaaagac ggaccattat ctgttcatga
     1081 aagttctcat ctccggcatg gcctcatggc atatggataa aacggctgcc tcctttgaaa
     1141 agctgggcat tttgtccggc atgtgggtaa gcaacaaaaa cagggggctt ccttctggga
     1201 aatataagcg catttggccc taccatttgg gcatgaaacc tttttatcat gttgcccctg
     1261 cttatcttga agaggagatg aggtggagaa acctttggct ttatgattgc tgggtaagga
     1321 ggcaggaact cccggcagat gtggatgtcg tgcagtgccc gatgggctct tgctcgcctg
     1381 tgtttgacct ggccgaagct tccggaagaa ccattttaaa ggtattcgaa gccatgaacg
     1441 ggcatccgac cacccagcgc ggctattggc agcgggaggc ggatattcat tcccccggct
     1501 tccgggttcc cgtaggggaa aaagtgtgga gccgcatgaa ccgggaaatt catcgggccg
     1561 attgtgttct atgcccaagc acctatgtta tggagagcat ggtggccaac ggcgtttcac
     1621 cggaaaaatg cctgctcaat cattttggct cagacacctc cctctttttc aaaagagaaa
     1681 aggaagacat cccttccttc cccaaattca tcattacggg gaacctgacg gttcggaaag
     1741 gccatcagta cctctttctg gcttttcaga aactggttga gcaatatccc gatgcggagc
     1801 tgcatgtgta cgggggcatc cggcccgatt tccggccgct gtgggagaaa tggaaaacct
     1861 gcccgcatct gtatcatcat gcgtccgtct cccagcagga actggcccgc cggctcgctt
     1921 cctgtacggc gtttatactt ccttccctgg aagaggggtt tgcccgttca ttgctggaag
     1981 ccatggccgt ggggctgccc atcattgcga cccacgagtc tggggctaca acgctgctgg
     2041 atgggcggtc cgccagaatc atccctgccg ccgatgcgga ggctatttac cgggccatga
     2101 aggaactgtg cgacaacccc gccttagtgc accgcctggg cgagcaggcg tatgcggcgt
     2161 ttggcggttc ggaaaatacc tgggaagcct acgccgggcg tcttgctgaa gcctaccggg
     2221 aacggttgga acgcctgcgg gaaagcgctg gacagcgggg ttcgcgctag ggaaggggcc
     2281 ggtttttccg ggcggaacac agggaactga ggagaagaag gtatttggcc gcgacccgag
     2341 agatgcgggc aaggaaaaaa gctgtatttt tccggggggt tttaaaattg accagcatat
     2401 ccatgtaata aggggcgatg ggcgaggcca gcaaaagccg atagtcatcc agtatggcgg
     2461 ccggcagggt ctccttcagg gatctggcgt cttcctcaag catggttgtt gcggcggtgt
     2521 ggctgatgcc cgtcgcatcg aaataagcga taaatgcgtt gatgggcttg aatgtggccg
     2581 actcttgaaa cagaagagaa agcagcattt tcctgtcggc gca
//
LOCUS       DAOMSU010000298         2621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4075_length_2621_cov_2.250974, whole genome shotgun sequence.
ACCESSION   DAOMSU010000298 DAOMSU010000000
VERSION     DAOMSU010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4075_length_2621_cov_2.250974"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..>2621
                     /locus_tag="PK077_09790"
     CDS             167..>2621
                     /locus_tag="PK077_09790"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRN24629.1"
                     /translation="MEKNGRFFEGKTSARPMIYAYEDASYPGCLKVGYTAVDVEKRVA
                     QQYPTLRPEGKKPYRVIFAEPALYEDGSSFTDHAVHRMLEKKGVEGVGGEWFRCTEKE
                     IRAAWLAVRTRTANMENRTRDFTMRPEQAEAVERTLNYFKAERADGRTPRFLWNAKMR
                     FGKTFAAYQLARRLNARRALILTFKPAVQTAWKEDLETHLDFEGWQFICREQGPDAQP
                     IDEQYRQADAGQPIVCFGSFQDFLGVNKETGGIKPQHEWVREINWDLVIFDEYHFGAW
                     KENAKALFLMEDEEEEYNTAPPETNTGNVCDEQDLPITADHYLYLSGTPFRALNSGEF
                     IEEQIYSWTYSDEQRAKAAWQGENNPYAALPRMVLLTYRMPEDIRQIAMGGEFNEFDL
                     NVFFQAEGKGNKARFIHEEYVQKWLDLIRGSHHAAATDELKLGGKKPPMPYSDTRLMS
                     LLNHTLWFLPDVASCHAMNNLLQQRQNTFYHDYQVIVCAGPEAGTGANALPPVLKAMG
                     GNPLESKTITLSCGKLTTGVTVKPWAGIFMLRNLSSPETYFQAAFRVQSPWTVDSDQP
                     GKKEIVKPNCYVFDFALDRALKQIADYSCRLNANPGNPETKVKEFISFLPVLAYDGSA
                     MKQVEAAEILDMAMAGTSATLLARRWESALLVNVDNVTLQRLLASREAMDALMSIEGF
                     RNLNSDLETIINKSDAVKGMRKGKDDLTPKEKKDLSQEEKEIKSLRRQIQQKLIKFAA
                     RIPIFMYLSDFREYCLKDVITQLEPHLFRKVTGLTEKDFELLVSLNVFNEALMNDAVY
                     KFKRYEDASLVYTGINRHAE"
BASE COUNT          690 a          688 c          748 g          495 t
ORIGIN      
        1 ccaaacaagt ctattccttc gtaccaatgc aggattttag taaggcgtgg acggatgaag
       61 aactttacgc aaaatatggt ttaacgggag aagaaataga attcatagaa agtatgatcc
      121 gtccgatgga aggttaacgg gagcaagaaa aagaggagca aaagtcatgg aaaagaacgg
      181 gcgttttttt gaagggaaga cgagcgcacg gccaatgatt tatgcctatg aggacgccag
      241 ctatcccggt tgtctaaaag taggatatac ggcggtggat gtggaaaaac gcgtagcgca
      301 gcagtaccct accttgaggc cggaagggaa gaaaccctac cgggttatct tcgcggaacc
      361 ggctctgtat gaagatggaa gcagctttac ggaccatgcc gtacaccgga tgctggagaa
      421 aaaaggcgtg gagggagtgg gcggagaatg gttccgctgt acggagaaag aaatcagggc
      481 ggcatggctg gcggtgcgca cccggacggc caacatggaa aaccgtacca gggattttac
      541 catgcgcccg gaacaggcgg aagccgtgga gcggacgctg aactacttta aagcggaacg
      601 cgcggatgga cgcacgccgc gttttctgtg gaacgccaaa atgcgctttg gcaaaacctt
      661 tgccgcttac cagctggcac ggcggttgaa cgcgcgccgg gcgctcatcc tgaccttcaa
      721 gccggcggtg cagacggcgt ggaaggaaga cctggaaacg cacctggact ttgaaggctg
      781 gcaattcatc tgccgggaac aagggccgga cgcccagccc attgacgagc aataccggca
      841 ggcggacgcc ggacagccta ttgtgtgctt cggctccttt caggactttc tgggggtgaa
      901 taaggaaacg ggcggcatca agccccagca tgaatgggtg cgggaaatca actgggatct
      961 ggttatcttt gacgaatacc actttggggc gtggaaggaa aacgccaaag ctctgttcct
     1021 gatggaggat gaggaagaag aatacaacac cgcgcccccg gaaaccaaca ccggcaatgt
     1081 ctgcgatgaa caggatctgc ctatcactgc ggaccactac ctgtaccttt ccggaacgcc
     1141 gttccgcgcc ctgaactccg gcgagtttat tgaagaacaa atttacagct ggacgtattc
     1201 cgatgagcag cgcgccaagg ctgcctggca gggggaaaat aacccgtatg ccgccctgcc
     1261 gcgcatggtg ctgctcactt accgcatgcc ggaggacatc cggcaaatcg ctatgggggg
     1321 agagttcaac gaatttgacc tgaacgtctt cttccaggcg gaagggaaag ggaataaagc
     1381 ccgttttatt catgaagaat acgtccagaa atggctggat ctgatccgcg gctcccatca
     1441 tgccgcggcg acggatgagc tgaagctggg cggcaaaaag ccccccatgc cttactcgga
     1501 tacccggctg atgagtttgc tcaaccatac cctgtggttc ctgccggatg tggcttcctg
     1561 ccacgccatg aacaacctgc tgcaacaacg tcagaatacc ttttaccacg actatcaggt
     1621 catcgtctgc gccggaccgg aagccggcac gggggcgaac gccctgccgc ccgtgttgaa
     1681 ggccatgggc ggcaatccgc tggaaagtaa aaccattacg ctctcctgcg gcaaattgac
     1741 cacgggcgtt accgtcaagc cctgggccgg catcttcatg ctgcgcaatc tctccagccc
     1801 ggaaacctac tttcaggcag ccttccgggt gcaaagcccg tggacggtgg acagtgatca
     1861 gccggggaaa aaagaaatcg tcaaacccaa ctgctacgtc tttgactttg ccctggaccg
     1921 cgcccttaag caaatagctg actacagctg ccgcctgaac gccaacccgg gcaatccgga
     1981 aaccaaggtg aaggagttca tcagctttct tcccgtgctg gcctacgacg gcagcgccat
     2041 gaaacaggtg gaagctgcgg aaatactgga tatggccatg gcgggaacca gcgccaccct
     2101 gctggccaga cgctgggaaa gcgccctgct ggtcaatgtg gataacgtca ccctgcagcg
     2161 cctgcttgcc agccgggaag ccatggatgc cctgatgagc attgaaggct tccgcaatct
     2221 gaacagtgat cttgagacca ttatcaacaa atcagatgcc gtcaaaggaa tgcgcaaagg
     2281 aaaagacgat ctgaccccta aggaaaaaaa agacctctca caggaagaaa aggaaatcaa
     2341 atccctgcgc aggcagatac agcagaaact gatcaaattt gccgccagaa tccccatctt
     2401 catgtatctg tcagacttcc gggaatactg cctgaaagat gtgatcaccc aactggagcc
     2461 gcatctgttc cggaaagtaa ccgggctgac ggaaaaagat tttgaactgc tcgtcagcct
     2521 gaacgtcttt aatgaagctc tgatgaatga tgccgtgtac aagttcaaac ggtatgaaga
     2581 cgccagtctt gtttatacgg gtatcaatcg ccacgcggag g
//
LOCUS       DAOMSU010000299         2617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4086_length_2617_cov_2.419594, whole genome shotgun sequence.
ACCESSION   DAOMSU010000299 DAOMSU010000000
VERSION     DAOMSU010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2617
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4086_length_2617_cov_2.419594"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..1004
                     /locus_tag="PK077_09795"
     CDS             102..1004
                     /locus_tag="PK077_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HRN24630.1"
                     /translation="MKYLNHNLLSRLGNLPLEARHSMTGNVSGRHRSASRGSSVEFAE
                     YRKYVAGDDTRRLDWKAYARSDRYYIKEFEADTNLRAYIVMDLSGSMNYHPEQVETKY
                     MRACRLAANLAYIAIRQGDAVGLSFARQTKDGAALHIPASRRPAHLNVLISQMDTHSP
                     QGETVLPDTLHELAERVGRRALVLIFSDLFTDTAELKNALRHLHFRKHDVAVFHLVDQ
                     LEIDFDFDRPIRFVDMEGGGSLITEPDLIADEYRAIVASYLEETRRICTDINADYRLV
                     GTGDSLEDVLTGFLMGRQKKKAAG"
     gene            1122..1694
                     /gene="pth"
                     /locus_tag="PK077_09800"
     CDS             1122..1694
                     /gene="pth"
                     /locus_tag="PK077_09800"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRN24631.1"
                     /translation="MDAPVKMIVGLGNPGRTYEDTRHNAGFMVLDRLAARWGAVFKAD
                     RQRKCEVAAGPGVLLVKPSTFMNESGLCVGPMMRFFKLDPRSVFVIHDEVDFPLGVIK
                     LREKGSAGGHNGMKSLIAHMGTQEFPRLRFGIGQPRGKGEMIGHVLGKFRPEERELLD
                     VTLGKAEDAVLYTMEHGITRAGNIFNAVPG"
     assembly_gap    2035..2044
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2045..>2617
                     /locus_tag="PK077_09805"
     CDS             <2045..>2617
                     /locus_tag="PK077_09805"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HRN24632.1"
                     /translation="RFWSFIRKLVLWCLVMAAIGAAVGYFGFMMAWKRYDNWAAEFDL
                     ERINDLEKPSIIYDRNGEEIGRIYVENRSYVTLDKISPAMINALIAQEDSRFREHPGY
                     DFLGILRAGREYIHNSGDANQGASSITQQLARNAYDLKNRAKARNEGSFGRKFVEIAL
                     ARRITERYSKDQVLEFYLNRVYFGSGFYGI"
BASE COUNT          556 a          784 c          765 g          502 t
ORIGIN      
        1 gccgcggaat cccagggcat cacgccccgt aaggccgtgg cgcgcctggc ggcggaaatc
       61 tgaccgccgt cccggcatca tcaatcccct ttccctgccc catgaaatac ctgaatcaca
      121 acctgctcag ccgcctgggg aaccttcccc tggaagcgcg ccacagcatg acggggaacg
      181 tatccggccg ccacagaagc gccagccgcg gctcctccgt ggagtttgcg gaataccgca
      241 aatacgtggc cggagacgat acgcgccgtc tggactggaa agcgtatgca cggtcggaca
      301 ggtattacat caaggagttt gaagcggata ccaacctgcg cgcctacatc gtcatggatc
      361 tttccggctc catgaattat catccggagc aggtggagac caagtatatg cgcgcgtgca
      421 ggctggcggc caacctggct tacattgcca tccggcaggg agacgcggtg ggactgagtt
      481 ttgcccggca gacgaaggac ggcgccgcgc tgcacatccc cgcctcccgc cgccccgccc
      541 acctgaacgt gctcatcagc cagatggaca cgcattctcc gcagggggag accgtccttc
      601 ccgataccct gcatgaactg gcggaacgcg tcggccgccg cgccctggtg ttgattttct
      661 cagacctgtt cacagatacg gcggagctta aaaacgccct ccgccacctc catttccgca
      721 agcatgacgt agccgtattc catctggtgg accagctgga aatagatttt gactttgacc
      781 gccccatccg cttcgtggac atggaaggcg gcggctccct gattacggag ccggacctta
      841 tcgcggacga gtaccgggct atcgtggcca gctatctgga agaaacgcgc cggatctgca
      901 cggatatcaa tgcggactac cgcctggtcg gaaccggaga ttccctggaa gacgtgctga
      961 ccggcttcct gatgggacgt cagaaaaaga aggcggccgg ataaatgccc ggctgtctca
     1021 cggaacggtt gattgctccg cggaacggat gcaggatgca cccagacgct ccccggattg
     1081 caggaagcgt ctgtttttca tctctttccc ttttttccat tgtggatgca cccgttaaaa
     1141 tgatcgtggg cctgggcaac ccgggcagaa cctatgaaga cacccgccat aacgcggggt
     1201 tcatggtgct tgaccgcctg gccgcccggt ggggggccgt attcaaggcg gacaggcagc
     1261 gcaagtgcga ggtggccgcc ggccccggcg tcctgctggt gaaaccctcc acgttcatga
     1321 atgaatccgg tctgtgcgtg ggccccatga tgcggttttt caaactggat ccccgcagcg
     1381 tgttcgtcat tcatgatgaa gtggatttcc ccctgggcgt gatcaagctg cgggaaaaag
     1441 gctccgcagg cggccacaac ggcatgaaat ccctcatcgc ccacatgggc acccaggaat
     1501 tcccgcgcct ccgtttcggc atcggccagc cccgtggcaa aggggaaatg atagggcacg
     1561 tgctgggcaa attccgcccg gaggaacgcg agcttctgga cgtgacgctt ggcaaggcgg
     1621 aggacgccgt gctgtacacc atggaacacg gcattaccag ggcaggcaat attttcaacg
     1681 ctgttccagg ctgacagccg catgaaaggc agccccattg gcgccatgac gcatccctgg
     1741 acgggccgga gagcgttttt tcctgaagac ggagcagccc gggcgcagga tgatacggct
     1801 gcctcaggcg cccggcggcg tccaggaata ctcagggggc gcgcatgcct tcttttttcg
     1861 cccgcccgcc atgtacggta ttgctaaaca aacgggaatc cgttaggatt tttgcacctt
     1921 ttcccctctc ctcaggacca atcctgcttc atcttcatgg ctgaattaag ttcacaagct
     1981 ccccgcaggc acatgcaggc caacaaaagg cggcatccca aggcaaagcc caggnnnnnn
     2041 nnnnaacgat tctggagctt tatccgaaag ctggtcctgt ggtgcctggt gatggcggct
     2101 atcggcgcgg ccgtgggata cttcggattc atgatggcct ggaaacgtta tgacaactgg
     2161 gcggcggaat ttgatctgga acgcatcaat gacctggaaa aacccagcat tatttatgac
     2221 cggaacgggg aggaaatagg ccgtatttat gtggagaacc gcagctacgt cacgctggac
     2281 aaaatctccc ccgccatgat taatgcgctg attgcccagg aagattcccg cttccgggaa
     2341 catccgggct atgacttcct ggggattctg cgggcaggcc gggagtatat ccacaacagc
     2401 ggggacgcca accagggcgc ctcctccatc acccagcagc ttgccagaaa tgcgtatgac
     2461 ctgaaaaacc gtgccaaagc caggaatgag ggcagctttg gccgcaaatt cgtggaaata
     2521 gcgctggcgc gccgcattac ggaacggtac agcaaggacc aggttctgga attttacctg
     2581 aaccgcgttt acttcggcag cgggttttac ggcatcc
//
LOCUS       DAOMSU010000300         2605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4117_length_2605_cov_2.302745, whole genome shotgun sequence.
ACCESSION   DAOMSU010000300 DAOMSU010000000
VERSION     DAOMSU010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4117_length_2605_cov_2.302745"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1858)
                     /locus_tag="PK077_09810"
     CDS             complement(<1..1858)
                     /locus_tag="PK077_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HRN24633.1"
                     /translation="MTDSPSKRLFILDGMALVYRAHFAFFSNPIRNSKGVNTSAVYGF
                     ANTLLGILEHERPTHIAACFDTSVPTARHKLYPAYKANRESMPEELSLQMPLIFRLLE
                     AMNIPILRYEGYEADDTIGTLARIADGTEGFQTYMVSQDKDLGQLISSTCFLWKPGKR
                     GNDHEVIDLAKLKEQWGIERADQVVDILALMGDSSDNIPGLPGVGEKTAKLLIGEFGS
                     VENLLSNTDKLKGKRKQIVEENGAMATLSKQLATIDRNVPLTVTLPELVKREPSPEEL
                     LALLQELEFRSMQAKLFGKKAPEARKSPLPADDLFAPAPQTEQPPLVEPSAPVSGARQ
                     SGSGQMDLFEERHLKTVDDFRHEYIIADTEEARSAMVAELEKHDSWCFDTETTGLNPL
                     TDNLLGVAFCAEPHKAWYMPVSGPEDLEAVKPLLEGPAEKIGHHLKFDLEVLRANGIH
                     VKGPFFDTLLAHALIAPGMKHGMDVLAENLLQYSTIKLKDIAAPGAKKRELDTSGVPV
                     EVMGKYSAEDADITLQLSSILKRQVKESGMEKLFRTVELPLLPVLADMEFSGIRVLPE
                     SLEKASVKVGAIIDGLRERIEEAAGHPLNLNSPKQLGDFLFGELELVKKPKKT"
     gene            2098..>2605
                     /locus_tag="PK077_09815"
     CDS             2098..>2605
                     /locus_tag="PK077_09815"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRN24634.1"
                     /translation="MTYTITPLIAEDAICERVRELAASIDRAMAGKPYILLLLLNGAL
                     PFAAMLEKHLKSRPAVKAVKISSYAGMRNSGSVVWEGDCGAFQPDVPVLVVDDVLDTG
                     ATLDEVCRELKRRGVKEVLTAVAVDKHCSRKVPFEADYVAFTQGNDFLVGFGMDLDGH
                     YRELPYIGK"
BASE COUNT          549 a          693 c          718 g          645 t
ORIGIN      
        1 tcgtcttctt gggcttcttc accagctcca gttctccgaa caggaaatct ccgagctgct
       61 tgggggaatt cagattcagg ggatggcctg cggcttcttc aattctttcc cgtaggccgt
      121 caatgatggc tcctaccttg acggaagcct tctccaggga ttccggaagc acgcggatgc
      181 cggaaaactc catgtccgcc agcacgggaa gcagaggcag ttccacggta cggaacagtt
      241 tttccatgcc gctttccttg acctgccttt tcaggatgga ggaaagctgg agggtgatat
      301 ccgcatcctc cgccgaatat ttgcccatta cttccacggg aacgccgctg gtgtccagtt
      361 cccgtttttt tgctcccgga gcagcaatgt ccttcagttt aatcgtggaa tattgcagca
      421 aattttctgc cagaacgtcc attccgtgct tcatgcccgg agcgatcagg gcatgagcca
      481 gcaatgtgtc gaaaaagggg cctttgacgt gaatgccgtt ggctctcaaa acctccaagt
      541 caaatttcag gtgatgccct atcttctctg cggggccttc cagaagcggt ttaaccgctt
      601 ccagatcctc cgggccggag acaggcatgt accatgcctt gtgcggttcc gcgcagaagg
      661 cgacgcccag cagattgtcc gtaaggggat tcaggcccgt cgtttccgta tcgaagcacc
      721 aggaatcatg cttttccagc tcggcaacca tggcggaacg ggcttcctcc gtgtccgcaa
      781 taatatactc gtgcctgaaa tcatctaccg ttttcaaatg gcgttcctca aacaagtcca
      841 tttggccgga accgctctga cgtgctcccg aaacaggggc ggagggttcc accaacggag
      901 gctgttccgt ctgcggggcg ggagcaaaca aatcgtccgc agggaggggg gattttctgg
      961 cctccggcgc ttttttcccg aacagcttgg cctgcatgga gcggaactcc aactcctgga
     1021 gaagggccag cagttcttcc ggactgggtt ctcttttaac caattcaggc agggtcaccg
     1081 tcaggggaac gttccggtca atggtggcca gttgcttgga aagggttgcc atggccccgt
     1141 tttcctccac aatctgtttg cgcttccctt tcagtttatc cgtattagac agcaggtttt
     1201 ccacggagcc gaactctccg atcaacagtt tggccgtctt ttcccccacg ccgggaagcc
     1261 ccggaatatt gtcggagctg tcccccatca gggccagaat atcaactacc tggtccgcac
     1321 gttcaatgcc ccattgctcc ttgagctttg ccaggtcaat cacttcgtgg tcattgcccc
     1381 ttttgccggg tttccacaga aagcaggtgg aggaaataag ctggcccaga tccttgtcct
     1441 gggaaaccat gtaggtttgg aacccctccg taccgtccgc aatgcgcgcc agcgtgccta
     1501 tcgtatcgtc cgcctcataa ccctcataac gcagaatggg aatattcatg gcctccagca
     1561 atctgaaaat caggggcatt tgtaagctca actcttccgg catggattcc cggttggctt
     1621 tataggcagg gtaaagctta tggcgcgccg tagggacgga agtgtcgaaa caggccgcga
     1681 tatgcgtggg gcgttcgtgc tccagaatgc ccagcagcgt attggcgaag ccgtacacgg
     1741 cggaagtatt gactcccttg gaattgcgga tgggattgga gaaaaaggcg aagtgggctc
     1801 tgtaaaccag ggccattcca tccagaataa aaaggcgctt ggaaggggaa tcagtcattt
     1861 ctgccttact ttaccatgcg gtccggcggg aagccaccgt taaatgcgcc tccgcattct
     1921 ctgccgatcg gggcggagaa acaatcttga gagcgctttt ctgatgtccc cctggctgtg
     1981 aagaccgatc ctgcttcttg taaaatttgc tggagtccgg ccgggaatgt tctgtctccg
     2041 ttcattctcc ttcaaggatt gattttctta caggaaacat tatcattctt catttccatg
     2101 acttacacca tcactccttt gattgcagag gatgccatct gcgagcgcgt cagggaactg
     2161 gccgcatcca ttgacagggc catggcagga aaaccctata ttttattgct gctgctgaac
     2221 ggcgctcttc ctttcgctgc gatgttggag aagcatttga aatcccggcc tgcggtgaaa
     2281 gccgtcaaaa tttccagtta tgccggaatg agaaattccg gttccgtggt atgggagggg
     2341 gattgcgggg ctttccagcc cgatgtgcct gttctggtgg tggatgacgt tctggatacg
     2401 ggtgctacgc tggatgaagt ttgccgggaa ttgaaaagaa gaggggtgaa ggaagttctc
     2461 actgctgtag ccgtggacaa acactgtagc cggaaagtgc cttttgaggc tgattacgtg
     2521 gcctttacgc agggaaacga tttcctggta gggtttggaa tggacctgga tgggcattac
     2581 cgggaactcc cgtacattgg gaaag
//
LOCUS       DAOMSU010000301         2577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4172_length_2577_cov_1.963125, whole genome shotgun sequence.
ACCESSION   DAOMSU010000301 DAOMSU010000000
VERSION     DAOMSU010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4172_length_2577_cov_1.963125"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="PK077_09820"
     CDS             <1..221
                     /locus_tag="PK077_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24635.1"
                     /translation="DILILVAIQKGCVTRKAILSDVPVNEGSFFGSIRSLLRNGFITR
                     KEDGRHHVNYRLTPKGESLIAKLYTVTK"
     gene            221..853
                     /locus_tag="PK077_09825"
     CDS             221..853
                     /locus_tag="PK077_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24636.1"
                     /translation="MEIKIDASDLLNQLDMLDERARKVDDLTVRDMKRRVPGAVSAAT
                     ASVYRIKKSEVASCSAYAYDHTGRGRNAGKAHVRTSFQGTTIASFAVAFKGRKHAEWK
                     TAPKRRPRTKITVRKNGVRRRVPKPYEVTVETFRGKPAVIKGKNGNRVFVVDGRNRAF
                     VVGKSNRPMVHASTSVPQAITNKKVKAIWMPKLNRDLESRFYHHFNRLMR"
     gene            1041..1634
                     /locus_tag="PK077_09830"
     CDS             1041..1634
                     /locus_tag="PK077_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24637.1"
                     /translation="MKPLQKSMKEEATKSEEAGKGYITAEKLAKLLDLSVRRVQALRA
                     DGAFVTEDTPMGKRYVFGASLVSYIKYLQNRQETSSLERQRLEAEVRCKKAKARIEEI
                     KLALLRGEVHKAEHVRTLMNGMVQETKAAFMAIPGRCAVDCAGASPNEAAIILRQAIF
                     GVFEELAAHTYDPSRFSDLMKEDGDQMSNGDEEEEES"
     gene            1679..>2577
                     /locus_tag="PK077_09835"
     CDS             1679..>2577
                     /locus_tag="PK077_09835"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRN24638.1"
                     /translation="MSLWLPPEDLSVDQWADKYRRLAGGLSAEAGQWRTNRTPYMREP
                     MCAFSDPNIEEIVFVAPSQVGKSELELNIIAYIIDQDPGTILYVQPRKEDAMSFSRLR
                     VAPMLKACDKIRDKVRDVEKKGRGATSTVLQKSFPGGMLTLVGSNSASDLSSQPVRYV
                     IGDELDRFALSAGRDGDPWELAKRRQNTFYNRKRVAVSTPTIKGASQIEFLYTQGTRE
                     RWKTRCPRCGEYHEVRFDDIQFKAKPKRIAGKESWYVDVTGWKCPGCGEVSEEQEVKK
                     SDSRWEAENPDAIRNNRCRSFWLG"
BASE COUNT          671 a          615 c          739 g          552 t
ORIGIN      
        1 tggatattct cattctcgtc gccattcaga aggggtgtgt aaccagaaag gctattctgt
       61 cagatgtgcc ggtcaatgaa ggatcattct tcggttccat ccgttccctg ttgagaaatg
      121 gctttatcac ccgcaaggaa gatggacggc atcacgtcaa ttacagattg actccgaaag
      181 gggaatccct gattgcaaaa ctctataccg ttaccaaata atggaaatca agatagatgc
      241 cagcgattta ttgaaccagc ttgatatgct ggatgagcgg gcacgtaagg tcgatgatct
      301 gacggtaaga gacatgaaac gtcgcgtgcc cggcgccgtc tctgctgcta ctgccagtgt
      361 gtacagaatc aaaaagagtg aagtggcgtc atgctcagcc tacgcttacg atcacacagg
      421 gagagggaga aatgccggaa aggcccatgt tcgtaccagt ttccaaggca cgacgatagc
      481 ctcctttgct gtagccttca aaggcaggaa gcatgcagag tggaagactg cccccaagag
      541 acggcccagg acaaagataa cggtccgtaa gaacggagta cggcgtagag tacccaagcc
      601 ctacgaggtg acggttgaaa cgttcagagg gaagccagcg gtcatcaaag gtaaaaacgg
      661 gaacagagta tttgttgtgg atggcaggaa tcgggcattc gtagttggta agagcaaccg
      721 cccgatggtt catgcgtcca cgtccgttcc tcaggccatt acaaacaaga aggtcaaggc
      781 tatatggatg cccaagctca atcgagactt ggagagccgg ttttaccacc atttcaacag
      841 attgatgcgg tgatgggaat cggaatggtc aaggggcggc ggccgagggg ccaaggtact
      901 gtccggcagc gttgctccat gcgctgggct ccaccccaaa taaggcgcat ttttcaattt
      961 ttttttcgtc gtttggttca ccccccatgc gtcgattttc ggaagagagg ggaaaaagca
     1021 cgaaatgctg ttgaaacggc atgaaaccgc tacaaaagag catgaaagaa gaggccacta
     1081 aatccgaaga agccgggaag ggttacatta cggctgagaa gcttgccaag cttcttgacc
     1141 tttccgtccg ccgtgttcag gcattgcgag cggacggggc gtttgtgacc gaggacacac
     1201 ccatgggaaa acgatatgtg ttcggagcat ccctcgtttc ctacatcaag tacctgcaga
     1261 accgccaaga gacttcttct ctggaacggc agcgccttga ggcggaagtc cgatgcaaga
     1321 aggccaaggc gcggattgag gaaatcaagc tggccctatt gcgtggcgaa gttcacaagg
     1381 ccgaacatgt tcgcactctc atgaatggaa tggtccagga aacgaaggct gccttcatgg
     1441 ctattcctgg gcgctgcgcg gtggattgcg ccggagcctc accgaatgag gcagcgatta
     1501 ttctgcgaca agccattttc ggggtatttg aagagctggc cgcccatacg tatgacccct
     1561 cacgattttc agacctcatg aaagaagacg gagaccagat gtccaacgga gacgaagagg
     1621 aggaagaatc atgaattccc gggagaaaag acatcttgca gatctataca atgaagcggt
     1681 gagtctgtgg ctgcccccgg aggatctctc cgttgaccag tgggcggaca agtaccgccg
     1741 tctggctggt ggattatctg cagaagccgg tcaatggagg acgaaccgaa ccccctacat
     1801 gcgggaaccg atgtgtgcct tttcagatcc gaacatcgag gaaatcgttt ttgtggctcc
     1861 ttcccaggtg gggaagtcgg aattggaatt gaatatcatt gcctacatta ttgaccagga
     1921 cccgggaacc attctatacg tccaaccgag aaaggaagat gccatgtcgt tttcccgtct
     1981 ccgcgtggct cccatgctga aagcatgcga taagattcgt gataaagtgc gtgacgtgga
     2041 gaagaagggg cggggagcaa cttctactgt tttgcaaaag tcctttccgg gaggtatgct
     2101 gacccttgtt ggttccaata gtgcgagcga tttgtcttct cagccggtgc gctacgtcat
     2161 cggtgacgag cttgaccgct ttgctttaag cgccggcaga gacggggatc catgggaatt
     2221 ggcaaaacgc cgtcagaata ccttttacaa ccgaaagcgc gttgccgttt ctactcccac
     2281 aatcaagggg gcgtctcaaa ttgaattcct ctatacgcag ggcacacgcg aacggtggaa
     2341 gacgcgctgc ccacggtgcg gagaatatca tgaggtgcgc tttgatgata tccagttcaa
     2401 ggccaaaccg aaacggatag cagggaagga atcgtggtat gttgacgtta ctggatggaa
     2461 atgccccgga tgcggggaag tcagcgaaga acaggaggtc aaaaaatcag attcccgctg
     2521 ggaagcggag aatcccgatg ccattcggaa taaccgttgc cggtcattct ggctcgg
//
LOCUS       DAOMSU010000302         2560 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4199_length_2560_cov_2.233533, whole genome shotgun sequence.
ACCESSION   DAOMSU010000302 DAOMSU010000000
VERSION     DAOMSU010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4199_length_2560_cov_2.233533"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="PK077_09840"
     CDS             <1..495
                     /locus_tag="PK077_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRN24639.1"
                     /translation="GERIHEKDEQGNPIDIGEVGNVIGCLTERITEALELQITPIVSP
                     LAKELGTHKPLNVNADLAAAALAKELKPVKLIYISDVPGIMKDPSDPSTLIKSVTRTE
                     ALDLIKDGTVSGGMIPKIHSAIDALNAGVRKVHFIDGRLPHTLLLEIFTPDGIGTEII
                     REQR"
     gene            504..869
                     /locus_tag="PK077_09845"
     CDS             504..869
                     /locus_tag="PK077_09845"
                     /inference="COORDINATES: protein motif:HMM:NF024114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HRN24640.1"
                     /translation="MKFSLFLPALFLLAASCSMQPTPASLSGSWIQPIPGQPGNVQGM
                     RLLPDGRAVSINMHTLLYQSWQLDGNRLTLTGKSMGNGNTSLFTATSTIDSLSKNTLI
                     LDTDGTRETYIRMPDADGR"
     gene            complement(888..1172)
                     /locus_tag="PK077_09850"
     CDS             complement(888..1172)
                     /locus_tag="PK077_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24641.1"
                     /translation="MMNFSIAKCLFSIGGCTTILGNISADTAVSSAGAIGGLGVLTIA
                     VNYTLNLLKKKDEQLMAKDARIEALMDRLIDKCPNCELAKAANKSLLDDE"
     gene            1409..2452
                     /locus_tag="PK077_09855"
     CDS             1409..2452
                     /locus_tag="PK077_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRN24642.1"
                     /translation="MHMADGFISPAVGCTMWAASAAITTLCARKVRQEKEMRLVPLMG
                     VLGAFIFACQMLNFTIPGTGSSGHLGGGLILAVLLGPYAGFLVMAAILAVQALFFADG
                     GLLALGCNMFNMGFVSCLVAYPFIYRPLAGKHPGTGRITLASVIAAVIGLQIGAFAVV
                     LETTASGISALPFAQFVELMLPIHLAIGAVEGLATAAVVIFISKAQPSLLRPADLETG
                     TVKKASWTVLGIFAVAALLCGAALSWFASAYPDGLEWSVAGVTGSEETRLAEPSPLHR
                     NLETVQEATAIMPDYAFPADDGANTAETESASSIVNPETSMAGVLGSAIIAALIFAAG
                     FLFGRKPHSHCPR"
BASE COUNT          521 a          844 c          665 g          530 t
ORIGIN      
        1 ggggaacgca tccatgaaaa agatgaacag ggcaacccca tagatatcgg tgaagtgggc
       61 aacgtcatcg gttgcctgac ggagcgcatt acagaagcgc tggaacttca gatcactccc
      121 atcgtctctc cgctggcaaa agaactgggc acccataaac cgctcaatgt gaacgcagac
      181 ctggcggcag ccgccctggc caaggaactc aagccggtga aactcatcta tatctccgat
      241 gtccccggaa tcatgaaaga cccttcggat ccctccaccc tcatcaaatc cgtcacgcgt
      301 acggaagccc tcgaccttat caaggacggc accgtatccg gcggcatgat acctaaaatc
      361 cactccgcca ttgacgccct gaacgcgggc gtgcgcaaag tgcatttcat tgacggacgc
      421 ctgccccata ccctgctgct ggaaattttc actccggacg gcatcggcac ggaaatcatc
      481 cgggaacaac gctgattcca gccatgaaat tttccctctt tctcccggcc ctgttcctgc
      541 tggcggcatc ctgctccatg cagcccactc ccgccagcct ctccggctcc tggatccagc
      601 ccatccccgg gcagcctggg aacgtgcagg gaatgcggct cctgccggac ggcagggccg
      661 tctccatcaa catgcacacg cttctctacc aaagctggca gctggacggc aaccgcctga
      721 ccctgacagg gaaaagcatg ggaaacggca acacctccct ctttaccgct acctccacca
      781 ttgacagtct cagcaaaaac acgctgattc tggacacgga cgggacccgg gagacgtaca
      841 tccgcatgcc ggacgcggac gggagataaa cgcccccacg ccagccgtca ttcatcatcc
      901 agcagggact tgttggccgc tttcgccagc tcgcaattcg ggcatttgtc aatcaggcgg
      961 tccatcaggg cttcaatgcg ggcatctttc gccatcaact gttcatcctt ctttttaagc
     1021 agattcagcg tatagttcac ggcaatcgtc agaacgccca ggcctccgat ggctccggcg
     1081 gaagacacgg ccgtgtctgc ggaaatattt cccaggatcg tcgtgcatcc ccctatggaa
     1141 aacaaacact tggctatgga aaaattcatc atgggttccg tctagcagac agttcttccg
     1201 cctgcatcac gcccaacccg tccacatctt ttgacatctt ttgcccggca tggacggcag
     1261 ctgaattgcc gccggacgca acatactttc ccccgttgcc gcatttcctt cttcggcttt
     1321 gtggcgttta tgctaaagtg gcgccgatct gcccggtacg gggctttcag caatacttca
     1381 cactattcaa tagaaaggca tcagcatcat gcatatggcg gacggcttca tctcaccggc
     1441 ggtaggctgc accatgtggg cggcttccgc tgcaattaca acgctctgcg cacgtaaggt
     1501 caggcaggaa aaagaaatgc ggctcgtccc gctcatgggc gtgctgggag ccttcatctt
     1561 tgcctgccag atgctgaatt tcaccattcc gggcaccggc tccagcggcc atctgggcgg
     1621 cggcctcatt ttggcggtcc ttctgggacc gtatgcgggg ttcctggtca tggcggccat
     1681 cctggcggtg caggcccttt tctttgcgga tgggggactg ctggccctgg gctgcaacat
     1741 gttcaacatg ggcttcgttt cctgcctggt ggcctacccc ttcatttaca ggcccctggc
     1801 cgggaaacac cccggcacgg gccgcatcac gctggccagc gtcatcgccg ccgttatagg
     1861 gctccagata ggcgccttcg ccgtcgtcct ggaaacaacg gcatccggta tttcagccct
     1921 gccctttgcc caattcgtgg aactgatgct gcccattcat ctggccatcg gcgccgtaga
     1981 agggcttgct acggccgccg tcgtcatctt catcagcaag gcacagccgt ccctgctgcg
     2041 cccggccgac ttggaaaccg gaaccgtcaa aaaagcatcc tggaccgttt tgggcatctt
     2101 tgcggtagcg gccctgctct gcggagccgc cctctcctgg ttcgcctccg cctatcctga
     2161 cgggctggaa tggtctgtag ccggagtcac cgggtcggaa gaaaccaggc tggcggaacc
     2221 gtcccccctc caccggaacc tggaaaccgt acaggaggcc accgccatta tgccggacta
     2281 cgcctttccc gctgatgacg gggcaaacac cgcagaaacg gaatccgctt cttccattgt
     2341 caaccctgaa acgagcatgg ccggcgtgct gggaagcgcc attatcgcag cccttatttt
     2401 tgcggccggt ttcctgttcg gcagaaaacc gcacagccac tgcccccgct gaccctcccc
     2461 atgccactgt ttacggacgc ctcgcagcaa ttccgccaga tggatcggtt ttcctgcgga
     2521 aataccggca tccaccgtct ggacgcccgc attaaaatcg
//
LOCUS       DAOMSU010000303         2554 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4215_length_2554_cov_2.225290, whole genome shotgun sequence.
ACCESSION   DAOMSU010000303 DAOMSU010000000
VERSION     DAOMSU010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4215_length_2554_cov_2.225290"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="PK077_09860"
     CDS             complement(<1..903)
                     /locus_tag="PK077_09860"
                     /inference="COORDINATES: protein motif:HMM:NF036565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HRN24643.1"
                     /translation="MIFSPLLKKGILLLALCGVLGGGVLSAQETGGSPITEEGLKALD
                     SNVFANEYMLALKPNVTRDRIAKIKDPFVRGLAEQMAEKKFDRKARALTAEPYEPVKD
                     LAERLRTSQYSKFENPTGIFFEEGEDVLLVMGDPKGEKLNLVIHNFGRDGGHSSYPLK
                     EGVNIIRAKNKGLGYIEYFTSNYKKAPKVHLSILSGKVNGVFVGGVSKNSDWKRMLEN
                     SPTEVVDIVGNRVHLVYPVEELKQFCPDKGEELIALYDRIIGMEQQIMGLYKYRMLPK
                     NRMFGRVIWNGFMHADGTGAAFHNG"
     gene            1075..>2554
                     /gene="htpG"
                     /locus_tag="PK077_09865"
     CDS             1075..>2554
                     /gene="htpG"
                     /locus_tag="PK077_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRN24644.1"
                     /translation="MNTETHQFQAEVRQLLDIVINALYSDREIFVRELVSNASDALEK
                     LRLKQLTDSNIYQPDKPLEITVTTDKENKTITIADTGIGMTEADLVENLGTIAHSGTK
                     KFMEALKQKQEGGADLIGQFGVGFYSSFMVADRVEVFTHSCEPEAASLRWSSDGREGY
                     SIETLAEPLDRGTRIVIRLKDEYEEFSQEYRVKELLRRYSNFVGFPLNSNGEHVNTVQ
                     AIWSKSKSDVKPEEYDEFYQFIAHTDEKPLSYMHFSADAPIALNALLFIPRRNPEMFG
                     FGRVDANVALYCKRVLIDAKPEGLLPEWLRFLNGVVDSEDLPLNISREMLQDNSLVRK
                     ISDIITKRFIKHLEKLAKDDQETYREFYAQFSRYLKEGVVTSWPNKESLGKLLRFEST
                     ATEPGETTSFEDYVTRMKEGQTAIYALTGPSRSHLENSPYLEAFKARGYEVAFFTDHG
                     DEFVLDSLSSVDGKPVTMIDRADVELPALEEEQKDALPQEEAA"
BASE COUNT          593 a          778 c          652 g          531 t
ORIGIN      
        1 accgttatgg aacgcggctc ccgtaccgtc cgcatgcatg aacccgttcc agatcacacg
       61 gccgaacatc cggtttttgg gcagcatcct gtacttgtac aggcccatga tctgctgctc
      121 catgccgata atcctgtcgt acagggcaat gagctcctcc cctttgtccg ggcagaattg
      181 tttaagttcc tccaccggat acaccagatg cacgcggttg cccacgatgt ccacaacttc
      241 cgtgggggag ttttccagca tcctcttcca gtcgctgttt ttgctgacgc ctcccacgaa
      301 aactccattc accttgccgg acagaatgga caaatgcacc ttgggtgcct tcttgtaatt
      361 ggatgtgaaa tactcaatat agccaagccc cttgttcttc gccctgatga tgttgacccc
      421 ctctttcagc ggataggagc tgtggcctcc gtcccgcccg aaattgtgga taaccagatt
      481 cagtttttca cctttgggat cccccatgac cagcaatacg tcctccccct cttcaaaaaa
      541 gatgccggtg ggattttcaa atttgctgta ctggctggtg cgcaggcgtt ccgccagatc
      601 cttgaccggt tcatacggct ccgccgtcag ggcgcgggcc ttgcggtcaa atttcttctc
      661 cgccatctgc tcggcgagcc cacgcacaaa gggatcctta attttggcga tgcggtcgcg
      721 cgtgacattg ggcttcaatg caagcatgta ttcattggcg aacacattgg aatccaatgc
      781 cttcaaaccc tcctccgtaa tcggcgagcc ccctgtttcc tgggcggaaa gcacgcctcc
      841 gcccagcacg ccgcagaggg ccagtaaaag aatccccttt ttcaacaatg gtgagaaaat
      901 catttgttaa ataatactgt tcggtttccg gaattgtttc aaggaaaaac gcccggcccc
      961 catgcctgtg aaaaagcctt tttgtgtgac atgggacgcc cgggcccagg gagaaaaact
     1021 gtccatcccc cttgacgggg gccgtataat gacgctcgca aaacattttt ccatatgaat
     1081 acagaaacac atcaatttca ggcggaagtc cggcagctgc tggacattgt catcaacgcc
     1141 ctttacagcg accgtgaaat ctttgtccgc gagcttgttt ccaacgcttc cgacgccctg
     1201 gaaaaactgc gtctgaagca gctgacggat tctaatattt accagccgga caagcccctg
     1261 gaaatcaccg tcaccacgga taaggaaaac aagaccatca ccattgcgga caccggcatc
     1321 gggatgacgg aagcggacct ggtggaaaac ctgggaacca tcgcccactc cggcacaaaa
     1381 aaattcatgg aggccctcaa gcagaagcag gaaggcggag cggacctgat tggccagttc
     1441 ggcgtgggct tctacagctc gtttatggtg gcggaccggg tggaagtgtt cacccattcc
     1501 tgtgaaccgg aagccgcctc cctgcgctgg tcttctgacg gacgggaagg ctacagcatc
     1561 gaaacgctgg cggaaccgct ggaccggggc acccgcatcg tcatccgcct gaaagacgaa
     1621 tatgaagagt tctcccagga ataccgcgtg aaggagctcc tgcgccgcta ctccaacttc
     1681 gtggggttcc ctctcaactc caacggagag cacgtcaata ccgtccaggc catctggtcc
     1741 aagtccaaat ccgacgtgaa accggaggaa tatgatgaat tttaccagtt cattgcccac
     1801 acggatgaaa aacccctgtc ctacatgcac ttcagcgcgg acgcccctat tgccctgaat
     1861 gccctgcttt tcatccccag gcgcaatccg gaaatgttcg gattcggccg cgtggacgcc
     1921 aacgtggccc tgtactgcaa gcgcgtgctg attgacgcca agccggaagg cctgctgccg
     1981 gaatggctcc gcttcctgaa cggcgtggtg gacagcgagg acctgcccct gaacatttcc
     2041 cgcgaaatgc ttcaggacaa ctccctggtg cgcaaaatca gcgacatcat caccaaacgc
     2101 ttcatcaagc atctggaaaa actggcgaag gacgatcagg aaacctacag ggaattctac
     2161 gcgcaattct cccgctacct gaaagaaggc gtcgtcacct cctggccgaa caaggaatcc
     2221 ctgggcaagc tgctccgttt tgaatccacg gccacggaac cgggggaaac gacctcattt
     2281 gaagactacg tcacccgcat gaaggaaggg cagacggcca tttacgcgct taccggcccc
     2341 tcccgttccc atctggaaaa cagcccgtac ctggaagcct tcaaagcccg cggctatgaa
     2401 gtggccttct tcacggacca cggggacgaa ttcgtgctgg actccctgtc cagcgtggac
     2461 ggcaagcccg tcacgatgat cgaccgcgcc gacgtggaac tccccgccct ggaagaggaa
     2521 cagaaggacg ccctgcccca ggaggaagcc gcgg
//
LOCUS       DAOMSU010000304         2546 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4237_length_2546_cov_2.081895, whole genome shotgun sequence.
ACCESSION   DAOMSU010000304 DAOMSU010000000
VERSION     DAOMSU010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2546)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2546
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4237_length_2546_cov_2.081895"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>870)
                     /locus_tag="PK077_09870"
     CDS             complement(<1..>870)
                     /locus_tag="PK077_09870"
                     /inference="COORDINATES: protein motif:HMM:NF025851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HRN24645.1"
                     /translation="RAIPAPMQVTAATPARVWTEGYGTGNGRLGILSFGVFPKETVVL
                     NEESIFAKKNFQMREGAAEALDKARELCKEGKYRSADQLFRKNILPPGNIAGDYQQGG
                     RLQVEFQGLPSPSSYQRTLDMRRGKATTRAQFGTGELTTEILAAPSSDCAAYHIACTM
                     PSGCRVSLNLEHPDPASRIVAQPNGWTLEGQGSNGGIRFENTVVILAPGASITRKGST
                     IILDSAREVLVLSSISTDYNIRKPETPLTHSLTAKNARILAKAQKAGWKKLAAETEDY
                     FSRLMTRCQVDLG"
     assembly_gap    871..910
                     /estimated_length=40
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1015..1335)
                     /locus_tag="PK077_09875"
     CDS             complement(1015..1335)
                     /locus_tag="PK077_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HRN24646.1"
                     /translation="MDAPVPIPVAMMAGFMILGAILSMGKCSFLIAGCNMMSREQKRQ
                     YDEQALCRFMGKIMYCLAFTMLLWLASILLQNSVLLSASLYFLVGSIAFAVIYAGTGR
                     RFKK"
     gene            complement(1504..2007)
                     /locus_tag="PK077_09880"
     CDS             complement(1504..2007)
                     /locus_tag="PK077_09880"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRN24647.1"
                     /translation="MPHLLSETLVWCVTLLLFGMGLIGTLIPMLPGIIIIAAGCVWQG
                     AMGRENLAWWEWTVLALLVAGGMVIDKLSGGMGAKKFGSTAAGIWGAIIGAVAGSLLF
                     SPIVGLLFMPFLGALLAELIFARKDIMDAFRAGSGAALGMLAGLLLEFTCGLLIIAWF
                     CSCCFLF"
     gene            2136..>2546
                     /locus_tag="PK077_09885"
     CDS             2136..>2546
                     /locus_tag="PK077_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRN24648.1"
                     /translation="MTSTGVYLCGCIMFFCVFSQGATSSPPAVSAADSSAVRELAHSL
                     KARVGMAAEMLDTGETVMVGDEAAYPMQSVVKFVLALSVLKRVDQGAMNLEQIIRIRP
                     EQLVKDTWSPLRERFPQGGDFSLKELLRVTVQESD"
BASE COUNT          555 a          660 c          747 g          544 t
ORIGIN      
        1 tctcccagat ccacctggca gcgcgtcatg agccgggaaa aataatcttc cgtttccgcc
       61 gccagttttt tccatcccgc cttttgcgct tttgccagga ttcgcgcgtt tttagccgtc
      121 agggaatgcg tcaggggagt ttccggcttc cggatattgt aatctgtgga aatggatgaa
      181 agcaccagca cttcccgggc ggaatcaaga ataattgttg agcctttgcg agtaatggag
      241 gctccgggag ccaggatgac caccgtgttt tcaaaccgga tgcctccgtt gcttccctgt
      301 ccttccagcg tccagccatt cggctgcgca acaattcggg aagccggatc cgggtgttcc
      361 agattcaggg aaacgcggca tccggacggc attgtgcagg caatgtggta ggcggcgcag
      421 tcgctggacg gggccgccag gatttctgtg gtcaattccc cagtgccgaa ttgggcgcgg
      481 gtggttgcct tgccccgccg catatccagc gtacgctgat aggaggaagg tgagggaagc
      541 ccctggaatt ccacctgaag acgccccccc tgctggtaat cccccgcaat gttgccgggg
      601 ggaaggatgt tcttccgaaa tagctggtcc gcactgcggt attttccttc cttgcaaagt
      661 tcgcgagcct tgtccagagc ttccgcagcg ccttccctca tttggaaatt tttctttgcg
      721 aaaatacttt cttcattgag gacgacggtt tccttcggaa aaacgccgaa agagaggatg
      781 cccagccttc cgttgcccgt gccgtatcct tccgtccata cccgggcggg ggtggcggcg
      841 gtgacctgca tgggggccgg aatggcccgg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      901 nnnnnnnnnn cgaaaaggca gagcaggaag gaatgtttca aggttgcagg gacgttcatg
      961 ggagaagata atgttgcgta taataaaagt aaaagaaagc tggtgtaaaa aacgctattt
     1021 tttaaatcgt cttccggtgc cggcgtaaat gactgcaaat gcaatgcttc ccaccagaaa
     1081 gtacagggag gcggacagga gcacggagtt ctggagaagg atgctggcaa gccagagcag
     1141 catggtgaag gcgaggcagt acatgatttt ccccatgaac cggcacagcg cctgttcatc
     1201 atactgtctt ttttgctctc tgctcatcat gttgcagcct gcgatgagaa aggaacattt
     1261 ccccatggaa agaatggctc caagaatcat gaaacctgcc atcatggcga cgggtatcgg
     1321 aacaggtgca tccataagct ggtaaaatga gggaatggga gaacctggtc attcgaccgg
     1381 gcgggcatgc ggggcggcgt tttgcggaat acgttgggga aactgtgttt ctttcagcaa
     1441 cggagaaact ccgttgttcc tgccgaaggc ttccgcaggg tacggttaca gttttttttg
     1501 acattaaaac aggaaacagc aggaacagaa ccaggcgatg atgagcaggc cgcaggtaaa
     1561 ttccagcagc aggccggcaa gcatcccaag cgccgctccg gaaccggccc tgaaggcgtc
     1621 catgatatcc tttctggcaa agataagttc cgccagcagc gcccccagga acggcatgaa
     1681 caaaaggcct acgatggggg agaaaaggag gctccccgcc acggcgccga tgatggctcc
     1741 ccagatgccg gcggcagtgc tgccgaattt tttggcgccc atgcctccgg aaagcttgtc
     1801 aatgaccatg cctcccgcca ccagcagcgc caggacggtc cattcccacc acgccaggtt
     1861 ttctctgccc atggctccct gccagacgca cccggccgca atgatgataa ttccgggaag
     1921 catggggatg agcgtgccga tcagccccat gccgaataac agcagggtga cgcaccagac
     1981 gagggtttcc gaaaggagat ggggcatgct ttccttctta tcaggaagcc ggacaggtgg
     2041 caaggtttct gcccggcttg cggatgacga agaaaggaaa aaagggggag aaattattat
     2101 tgcggttgtt gcgggcgtgg tatggaatgg cgcctatgac ttccaccggt gtttatctat
     2161 gcggttgcat catgttcttt tgcgtttttt ctcagggggc cacgtcttct ccgccagccg
     2221 tttccgcagc ggattcctcc gccgtacgcg aactggccca ttccctgaag gcccgtgtgg
     2281 gcatggctgc ggaaatgctg gatacggggg aaaccgtcat ggtgggggat gaggcggcct
     2341 atcccatgca gagcgtcgtc aaattcgtgc tggccttgtc tgtcttgaag cgggtggacc
     2401 agggcgccat gaatctggaa cagatcatcc gcatacgtcc ggaacaactg gtgaaagata
     2461 cctggagccc tctccgggag cgttttcccc aaggcggcga tttctctctg aaggaactgc
     2521 tgagggtgac tgtccaggaa agcgac
//
LOCUS       DAOMSU010000305         2535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4267_length_2535_cov_3.359677, whole genome shotgun sequence.
ACCESSION   DAOMSU010000305 DAOMSU010000000
VERSION     DAOMSU010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4267_length_2535_cov_3.359677"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(137..766)
                     /locus_tag="PK077_09890"
     CDS             complement(137..766)
                     /locus_tag="PK077_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24649.1"
                     /translation="MRRIVSLPVLAAFVVLGASCSQQVQQPVKKAVPPPVYVGKVEQV
                     YPNHHYVLIALAGNVYEPGTVLISQSPGREEKCRVANLIVTEERMGRARIPADIRSGA
                     VEAGDLVFLYRNLAVPESTGKMDEQKPVSPEPETDEDSKVVAPPIAPDGGRTADGLMP
                     LPGEQPVPASADQERISREREKILRELESVPDRLEEPWSRTFSGKPVER"
     gene            complement(786..>2535)
                     /locus_tag="PK077_09895"
     CDS             complement(786..>2535)
                     /locus_tag="PK077_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRN24650.1"
                     /translation="LYHEAAAVAPSKPEDSVPPPSAPRTQGRLPLAPRPRITVAEPAE
                     MKPAPKEQPFSKLSTPPTEEFREYELPPFELLHYEEKPEGPTAEDKEEMLEIQQKIID
                     TLTTFRVDVTPGDITRGPTITRYEVYPARGVRVNTFDQYAKDIALATKAESVNIVAPI
                     PGKDTVGIEIVNRKKVAVPLRELLQDPAFCSPKKKIPLALGKDVYGRTVIGDLASMPH
                     LLVAGATGSGKSVCINSIISSMLLKFRPDELRLILVDPKVVEMQPYSKLPHLIVPVVT
                     DPKKVPNALRWCVNEMEHRYHCFAKVGVRNFEAFNKRPPDVPAQETEEPEDGQVDESL
                     AESIARDLESQGEWPVEEDDELDLEDDGVIPERFPYIVIIIDELADLMQTVGADIETN
                     IGRLTQKARAAGIHLIVATQTPRRQVVTGTIKANIPTRIAFQVASGTDSRVILDRQGA
                     EKLVGKGDLLYLPPGSAQVERAQGAFISDDEVEALVAHCASQAKQKFHEEVQKSLDEP
                     SRGGADSPLDDAEEECYAKCLEVAVVERKVSTSLLQRRLSIGYGRAARMMDLLESRGI
                     IAPADNTNRPRKVLVE"
BASE COUNT          515 a          794 c          652 g          574 t
ORIGIN      
        1 atcgggttta tcaggaatac tggaacaggt catccgtccg tttcaaagaa actggtattt
       61 gaattcccaa tacccggcct catcttcagg agctccttcc cgccctggga aacattgcgg
      121 atacctccgc atccggttat ctttccaccg gttttccgga gaaagtcctg ctccacggtt
      181 cttccagcct gtccggaacg ctttccaact cccggagtat tttctcccgc tctcgggaaa
      241 tccgctcctg atccgcagag gccggaacag gctgctcccc gggcagaggc atcagcccgt
      301 ccgccgtcct gccgccgtcc ggggcaatcg gcggagcaac aaccttgctg tcttcatccg
      361 tttccggttc agggctgacc ggcttctgct catccatctt tccggtactt tccggaacag
      421 ccaggttccg atacaggaac accaggtccc ccgcctccac agcgccgctg cggatatccg
      481 caggaatgcg cgcacgcccc attcgctcct ccgtcacaat caggttggcg actctgcatt
      541 tttcttcccg gcccggagat tgggagataa gcacggttcc cggttcataa acattcccgg
      601 ccagagcaat cagcacataa tgatgattag gatacacctg ctccaccttg cccacatata
      661 cggggggagg gactgctttc ttaacgggct gctgcacctg ctgggaacag gaggccccca
      721 gcaccacaaa cgccgccaga acaggcaggg aaacaattcg tctcatgggg agttttttca
      781 tcgtttcatt ccaccagcac cttgcgtgga cggttggtat tatccgccgg ggcaataatg
      841 ccgcgggatt ccagcaaatc catcatgcgc gctgcacggc cgtatccaat gctcaaacgc
      901 cgctgcagca gggaggtgct taccttacgt tccacaacag ccacttccag gcacttggca
      961 tagcattcct cttccgcgtc atccagcgga ctgtccgcac ctccgcggga aggctcgtcc
     1021 agggacttct gaacctcttc atggaatttc tgcttggcct gggaagcaca gtgggccacc
     1081 agggcttcca cttcatcgtc agagataaag gctccctgcg cccgctccac ctgggcggaa
     1141 cccggcggca aatacagaag gtctcccttg cccaccagtt tttccgcccc ctgcctgtcc
     1201 agaatcacac ggctatccgt accgctcgcc acctggaaag cgatgcgcgt cgggatattg
     1261 gccttgatcg tccccgtcac cacctgcctt cttggcgtct gcgtcgccac aataaggtgg
     1321 atgcctgcgg cacgggcctt ttgggtaaga cgtccaatat tcgtttctat gtccgctcct
     1381 actgtctgca tcaggtccgc aagttcgtca ataataatga cgatgtaggg gaagcgttca
     1441 ggaataactc cgtcgtcctc cagatccagt tcatcatctt cttccacagg ccattccccc
     1501 tgggattcca ggtctctggc aatggattcc gccaaagatt catccacctg cccgtcttcc
     1561 ggttcctccg tctcttgcgc aggaacatcc ggcgggcgct tgttgaaagc ttcaaagttt
     1621 cgcacaccca ccttggcaaa gcaatgatag cggtgctcca tttcattcac gcaccaacgc
     1681 agggcattgg gaactttctt gggatccgtc accaccggaa caatcagatg cggcagcttg
     1741 gaataaggct gcatttccac cactttcgga tccaccagaa taagcctcag ttcatccgga
     1801 cggaatttca ggagcatgga ggaaataatg ctgttaatgc acacggattt tccggatccg
     1861 gtggcgccgg ccaccaacag gtgcggcatg gacgccaagt ccccgatcac ggtacgtcca
     1921 tacacgtcct tccccagcgc cagcggaatc tttttcttgg gggaacaaaa agcgggatcc
     1981 tgcaaaagtt cccgcagggg aacggccacc ttcttccggt tgacgatttc aatacctacc
     2041 gtatccttgc ccggaatggg ggccacgatg ttcacgcttt ccgccttggt cgccagcgca
     2101 atatccttcg catactggtc aaaagtattt acgcgcacgc cgcgcgcggg atacacttca
     2161 taacgggtga tggtcgggcc tcgggtaata tccccaggcg tcacgtccac gcggaatgtc
     2221 gtcagggtat caataatctt ctgctgtatt tccagcattt cttccttgtc ctctgccgta
     2281 gggccttccg gcttttcttc ataatgcagc agttcaaagg gaggcagctc atactcgcgg
     2341 aattcctccg tgggaggagt ggacaatttg gaaaaaggct gctctttggg cgcgggcttc
     2401 atttccgccg gttccgcaac ggtaatcctg gggcgcggag ccaggggaag gcggccctgg
     2461 gttcgcgggg cgctgggagg cggaacagaa tcctccggct tggacggagc aaccgccgcc
     2521 gcttcatgat acaaa
//
LOCUS       DAOMSU010000306         2523 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4299_length_2523_cov_2.570502, whole genome shotgun sequence.
ACCESSION   DAOMSU010000306 DAOMSU010000000
VERSION     DAOMSU010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4299_length_2523_cov_2.570502"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..702
                     /locus_tag="PK077_09900"
     CDS             115..702
                     /locus_tag="PK077_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRN24651.1"
                     /translation="MFGSFTTVPAPKGNKNPESTPSWMDVAKSQDIPEPVAEAAPSYA
                     PTTRVQQLTRNVLNSDVEVIGSLRFSDDLLIDGTVEGDISSEGVLSVGQNAVIRAEIN
                     TKSVIIHGKVIGNVTVTDRVELKSTAELVGDIQAASLAIEGGAIFIGHSTVGAPTVGT
                     TGASAAKKAASVHAFAPEPETHAPASQSTLDIDAE"
     gene            773..1411
                     /locus_tag="PK077_09905"
     CDS             773..1411
                     /locus_tag="PK077_09905"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRN24652.1"
                     /translation="MNPTFHPIPRISTAPSGKISHPSPAPPWTLPPTAESSLAPAREV
                     ECFSCHKSTSVPATAVSARCGHCSAYIKLDDVILHSRTHRTKVQTCGSVTVQANADLK
                     GLNIECRDLVLYGRASGDFLCRGVCKIKTDQHISGSICARRMVVEKKTTVLVTGTIRV
                     ENIWIQGSLEGTLTADETVTIHRHAKFLGDITARRLIIEEGGTHQGAFTRLT"
     gene            1413..>2523
                     /locus_tag="PK077_09910"
     CDS             1413..>2523
                     /locus_tag="PK077_09910"
                     /inference="COORDINATES: protein motif:HMM:NF036484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24653.1"
                     /translation="MNLPISIRGARQHNLRNLDLDLPSNKLIVFCGPSGSGKSSLAFD
                     TLFSESRRRFLDCLSARSRQGMDQPEKPEVDSITGLPPALCLEQSARQQSSRTLLGSI
                     TEILDYLRILYAAAGTPHDPETGKELERKSPDRITEELVSLPEHTRLILAAPAENLLA
                     QDPAATLGDFQRQGFLRVYWNGEMRDIEEISSPVPPPPDAALVIDRLIVRGENTASRI
                     ADSLQTALRINPDEVRAIITIPGEEASIRAFHTRYRNPETGFLLPQLTPRHFSFNSPL
                     GACPSCRGTGLNEQENGPCRACGGQRLSPLALAVTMPAPDRAYNLAELTALPLEDMAG
                     ELERLKTPPSLAAALTPLMEEINKRVRFLNELGLSY"
BASE COUNT          538 a          848 c          666 g          471 t
ORIGIN      
        1 tttttttatc cctgccggag aaaattgttt cacatccaca tgcgggtgat gtacattaaa
       61 ccggacgagt ttcaagagct cccatcttac ctttcacccc ctctttttaa cgccatgttt
      121 ggatcattca ctaccgttcc cgcccccaag ggcaataaaa atcctgaatc cacccccagc
      181 tggatggatg tagccaagag ccaggacatc cccgaaccgg ttgcggaagc cgcgccttcc
      241 tacgctccca ccacccgtgt ccagcagctg acccgcaatg tgctgaactc cgatgtggag
      301 gtaatcggtt ccctgcgttt ttccgatgac ctgctgattg acggcaccgt ggaaggcgac
      361 atttcctccg aaggggtgct ttccgtaggc cagaacgccg tcatccgggc ggaaatcaac
      421 accaaatccg tcatcatcca cggcaaggtc atcggcaatg taacagtcac ggaccgcgtg
      481 gaactgaaaa gcacggcgga actcgtaggt gacatccagg ccgcctccct ggctattgaa
      541 ggcggagcaa tcttcatcgg ccattctacc gtaggcgccc ccacggtagg aaccacaggg
      601 gcttccgccg ccaagaaggc ggcttccgtc catgccttcg ctccggagcc ggaaacccat
      661 gctccggcca gccagagcac gctggatatt gacgcggaat aaaaaactct ccgcctgcta
      721 ctttcagggg cggcaaaagg acggtccgtt cttttgccgc cctctttgtt ccatgaaccc
      781 caccttccat cccatccccc ggatcagcac cgcgccctcc gggaaaatca gccacccctc
      841 cccggcaccg ccctggacac tccccccaac tgcggaaagc agcctggcgc ctgcccggga
      901 ggtggaatgc ttctcctgcc acaaaagcac ctccgtcccc gccacggcag tttccgccag
      961 gtgcggccat tgctccgcgt atatcaaact ggacgacgtc attttacaca gcagaacgca
     1021 ccggaccaag gtgcagacct gcggaagcgt taccgtgcag gccaatgcag atctgaaggg
     1081 gctgaacatc gaatgccgtg acctggtctt atacggcagg gcttccggcg acttcctgtg
     1141 ccgcggcgtc tgcaaaatca aaacggacca gcacatctcc ggctctatct gcgcccgcag
     1201 gatggtggtg gaaaaaaaga cgacggtgct ggtcacagga accatccgcg tggaaaacat
     1261 ctggatacag gggtctctgg aaggaacgct tacggcggat gaaaccgtca ccatccaccg
     1321 gcacgccaaa ttcctgggag atatcacggc gcgccgcctc atcattgagg aaggaggcac
     1381 gcaccaggga gcctttaccc gccttacgtg acatgaatct tcccatctcc atccgcggcg
     1441 cgcgccagca caacctccgg aacctggatc tggatcttcc gtccaacaag ctcatcgtat
     1501 tctgcggccc ttccggctcc ggaaaatctt ccctggcgtt tgatacgctt ttttccgaat
     1561 cccgcaggcg ttttctggac tgcctgtcgg cacgctccag gcagggcatg gatcaaccgg
     1621 aaaaaccgga agtggacagc attaccgggc tgcccccggc cctgtgcctg gagcaatccg
     1681 ccaggcagca gagttcccgc accctgctgg ggagcatcac ggaaattctg gactacctgc
     1741 gcatccttta cgcggctgcc ggcacgcccc atgacccgga gacaggaaag gaactggaac
     1801 gcaagagtcc ggaccggatt acggaggaac tcgtttccct gccggaacac acgcgcctga
     1861 ttctggccgc tccggcggaa aacctgctgg cccaggatcc cgcagcgacg ctgggcgact
     1921 tccagcggca gggcttcctc cgggtttact ggaacggaga aatgcgggat attgaagaaa
     1981 taagttcccc cgtccccccg cctccggacg cggccctggt cattgaccgc ctcatcgtca
     2041 gaggggaaaa tacggcctcg cgcattgcgg attccctgca aacggctctc cgtatcaatc
     2101 cggacgaagt gcgggccatc atcaccatac caggagagga agcctcaatc cgggccttcc
     2161 acacccgcta ccgcaatccg gaaacaggct tccttctgcc ccagcttacg ccccgccatt
     2221 tttccttcaa ctccccgctg ggggcatgcc cctcctgccg gggaaccggc ctgaatgaac
     2281 aggaaaacgg tccgtgccgc gcctgcgggg gccagcgtct ttcccctctg gccctggccg
     2341 tcaccatgcc tgcgccggac cgggcctaca atctcgcgga actgacggct cttcctctgg
     2401 aagatatggc aggagaactg gaacgactga aaacgccccc ctccctggcg gcggcattga
     2461 ccccgctcat ggaggaaatc aacaaacgcg tgcgtttcct gaatgagctg ggactctcct
     2521 acc
//
LOCUS       DAOMSU010000307         2508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4322_length_2508_cov_2.431716, whole genome shotgun sequence.
ACCESSION   DAOMSU010000307 DAOMSU010000000
VERSION     DAOMSU010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4322_length_2508_cov_2.431716"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..607)
                     /locus_tag="PK077_09915"
     CDS             complement(<1..607)
                     /locus_tag="PK077_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009963455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRN24654.1"
                     /translation="MNITYPDLPISRRRNDILAAMREHQVIVVVGETGSGKTTQLPKM
                     AMELAGEARGRVGCTQPRRLAAASVSRRVAEELNCDLGGLVGYQVRFEEKAGPDTRLK
                     FMTDGILLAETQHDPDLRQYHTLILDEAHERSLNIDFLLGYIRQILPRRPDLKVIVTS
                     ATIDADRFAKHFAMPRPALGRPKPGGAPSGGSAAHEVAERGG"
     gene            complement(675..1157)
                     /gene="ilvN"
                     /locus_tag="PK077_09920"
     CDS             complement(675..1157)
                     /gene="ilvN"
                     /locus_tag="PK077_09920"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HRN24655.1"
                     /translation="MTRHTISVLVENKFGVLARIAGLFSGRGYNIHSLNVAPSQDPRF
                     SRMTIVVREKEDVLDQIIKHLEKLVNTVEVVDFRNTDNVYRETVLTRIGVDSATRHEV
                     IEFCQILGAHIVDVTRDALTIEVTGGEYKIDRFLSLIEDYDVQMLTRSGRIALPKPRQ
                     "
     gene            complement(1279..>2508)
                     /locus_tag="PK077_09925"
     CDS             complement(1279..>2508)
                     /locus_tag="PK077_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRN24656.1"
                     /translation="QPSEDGDFMIEKRLTVHGLGSGVIVTEEGHIITAYHVIQNKHAL
                     RVTLSNGKSVSVRLVGVDPELDIAVLQVENPMTFTPLPFGNSDEIAPGMIVLACGNPY
                     GLGTTVSRGIISARERKLVDSGINLIQTDASIFPGNSGGPLINIRGEIIGINKSVLPN
                     AEKNYAGIGFAIPSNLVMHSFEQICKHGRPMKGYLGLDIVRNTPPLRSFLDYHEAGGA
                     VINIVKHGSPAEQAGLQTGDVILSFNEVPIQTDEEVKERIENLSIGDKFRLKVWRKGQ
                     KMNVTLKVGDSILKNVPSPWEDFMEGVGMHLRELTAEEQSIGARGLLVIQVNPKKSVG
                     QILQAGDMVFAVNHQSVSTKEALVKALREGPALLTVSRDGQQFNVKVDARPVSEKTLL
                     PRIPEATKGNAIIPHEL"
BASE COUNT          569 a          710 c          643 g          586 t
ORIGIN      
        1 cgcccccacg ctccgccact tcgtgtgctg cgctgccccc cgagggggcg ccgcccggct
       61 tggggcggcc cagcgccggg cggggcatcg caaagtgttt ggcaaagcgg tcggcgtcaa
      121 tcgtggccga ggtgacgatg actttcaggt ccggcctacg cggcaggatc tggcggatat
      181 agcccagcag aaagtcgatg ttgaggctgc gttcatgcgc ctcatccaaa atcagggtat
      241 gatattggcg cagatccgga tcatgctgcg tttccgccag aagaatccca tccgtcatga
      301 acttcaggcg cgtatccggc ccggcttttt cctcaaagcg cacctggtaa cccaccaaac
      361 ctcccaggtc gcagttcaat tcttccgcaa ctcggcggga aacggaagca gcagccagcc
      421 ttctgggctg ggtgcaccct actctgcccc gcgcctcccc ggcaagctcc atggccattt
      481 tagggagctg ggtagtctta ccggatcccg tctcccccac tacgacaatg acctggtgct
      541 cccgcattgc cgccaaaatg tcattccggc ggcgggaaat gggtagatcc ggataagtaa
      601 tattcatggt gaagatgaga aaaaaccctg caagaacgtc ctgcagggtt gaagaagaaa
      661 accgggaggt tcttttactg acggggtttg ggaagggcga tacggccgct ccgggtcagc
      721 atctgcacat cataatcctc aatgagggac aggaaacggt ctatcttgta ctcgcctccc
      781 gtcacttcaa tggtcagggc gtcccgcgtg acatccacaa tgtgggcgcc cagtatctgg
      841 cagaactcaa tgacttcatg ccgcgtggcg gaatctacgc caatgcgggt caataccgtt
      901 tcccggtaca cattgtccgt attgcggaaa tctactactt ccaccgtgtt gaccagtttt
      961 tccagatgct tgataatctg gtccagaaca tcctcctttt cgcgtacgac gatggtcatc
     1021 cgtgaaaagc gcgggtcctg gctaggggca acgttcaacg aatgaatatt gtagccccgt
     1081 ccactaaaca aaccggcaat gcgggcaagc acgccaaatt tgttttcaac aagtacggag
     1141 atagtatgac gagtcataat ttaatttctt aaaggtgatg tattataagc acttttgact
     1201 gccagtcaag cctgcggacg gcgatgccgg aaaacgtttt tcctaacgga ggttccggca
     1261 ggaaccactc aggccggatc aaagctcgtg cggtatgatg gcgttgcctt ttgtcgcctc
     1321 cggaatgcgg ggaagcaatg ttttttcaga aacggggcgc gcatccactt tcacattgaa
     1381 ttgctgcccg tcgcgggaaa cggtcagcaa tgccggtcct tccctaagag ctttgaccaa
     1441 ggcttccttg gtgctgacac tctggtggtt tacggcaaag accatgtcgc cggcctgcaa
     1501 tatctgccct acggatttct tcggattcac ttgaatgacc agcaaccccc gagcacctat
     1561 ggattgttct tccgcagtca gttcacgcaa atgcattccc actccttcca tgaagtcttc
     1621 ccaaggagaa ggaacatttt ttaaaatact atctcccacc ttcagggtga cgttcatttt
     1681 ctgccctttg cgccagactt tcagccggaa cttatcgccg atggaaagat tttcaatgcg
     1741 ttccttcact tcctcatccg tctggatggg gacttcgtta aaactcagta ttacgtcacc
     1801 tgtttgaagg cctgcctgct ccgccgggga accatgcttc acgatattga ttactgcgcc
     1861 tcccgcctca tgataatcca gaaagctgcg aagcggggga gtattgcgca caatgtccaa
     1921 tcccagatac cctttcatgg ggcggccatg tttgcaaatc tgctcaaagc tgtgcatcac
     1981 cagattggag ggaatggcaa acccaatgcc ggcgtaattt ttctccgcat tgggaagcac
     2041 cgatttattg atgccgatga tttccccgcg gatgttgatg aggggacctc ccgaattccc
     2101 cggaaagata ctggcatccg tctggatcaa gttaatgccg gaatccacca gcttgcgttc
     2161 acgggcactg ataatgccgc gggaaacagt cgtgcccagc ccatagggat tgccgcatgc
     2221 cagaacaatc atgccgggcg caatttcatc actgttgccg aacggaagag gggtaaacgt
     2281 catcgggttt tctacctgca gcacggcaat atccagttcc ggatccaccc ctaccaggcg
     2341 tacggaaacg ctttttccat tgctcagcgt tacgcgcagc gcatgcttgt tctgaatgac
     2401 atggtaggcg gtaataatat gcccttcttc agtaacaatg acgccggaac ccaaaccatg
     2461 aaccgtcagc ctcttttcaa tcatgaaatc accatcctcg gaaggctg
//
LOCUS       DAOMSU010000308         2499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4346_length_2499_cov_2.331833, whole genome shotgun sequence.
ACCESSION   DAOMSU010000308 DAOMSU010000000
VERSION     DAOMSU010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4346_length_2499_cov_2.331833"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..564)
                     /locus_tag="PK077_09930"
                     /pseudo
     CDS             complement(1..564)
                     /locus_tag="PK077_09930"
                     /inference="COORDINATES: protein motif:HMM:NF015585.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
     gene            complement(1012..2112)
                     /locus_tag="PK077_09935"
     CDS             complement(1012..2112)
                     /locus_tag="PK077_09935"
                     /inference="COORDINATES: protein motif:HMM:NF024770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRN24657.1"
                     /translation="MTSLPASSLVETVANKIKQMILSGSLVNILPGERELGHRLSVGR
                     ETIRKALALLERDAWIAPPRIKVPRRILKTTEEETVKNIFCHPVQEKKEILGFLTPQP
                     LKRLAQSVLAEIYTISKILEEDGISVRIFEAPWILGNNPDKRLAKLVTKSECVCWILH
                     RSSEQTQLWFKTHRIPCIVRGTSYQSSNLPYLDRHWAATTHHAAQHLWNKGHRTVGLC
                     LPPDPLKGHQLMQKGFFSFAEQGWNPILVPTPFETPLFFEYLAKAFKKHPDMSALVAT
                     RGNQIVPILSWVEARSLSIPDQLSLVSLTYEPFMERLLPTITYYEENQTKTVHKLIRM
                     LRSLTAGKNIKSISVIPEIYPGQSVSLHAPSA"
BASE COUNT          529 a          578 c          707 g          685 t
ORIGIN      
        1 gcggaggcaa agcgttccca gggccagaat tccatgcgta cccagatgga cggtatccgt
       61 gggatagggg gtgcggaagt ccacgctgcg cagctggaga tcgtctgcgg ggacataaac
      121 cgcattgttg acggagaggg aggtacggga ttcttccgcc acatcctttt gcccctgccg
      181 cacttcttcc ctgcccggaa aacgggccgg agcgggcacg ggaagaacgc tgccgtagat
      241 aaaggccaga ccgggggctt ccagatccgc tctgatgcgt ttaatcaggt tgttcaggtt
      301 ggcgccgtaa gacagagcgt ttttgatgcc ggcaatgtcc cttgcgtctc cttccccctg
      361 ttgccacacc atggctttca ggacaggagc atgtccctgt tttttcaggg cttccattcc
      421 gttgcggacg gtacggatca gtttgacgta ttcttccccc tgcttatcct gagaagtttt
      481 tcctgggttc cattgccgga aaaggttgct tccgctccgg gcgtgtttga tgatggccca
      541 tttttttgcg ggaatttttt ctgcaacgct gttcccaggc ttagttccac gccaaaacgg
      601 tcaggggatt cggatgccgg gctcaagcga acaagctgtt ctgccggtcc tgtccctttg
      661 agggagccgg aatagtacag aagaatccgt ttatccgtct tgaagcaggg aggaatgttg
      721 ttgacatatc cttgccctgt ggcgtttgac tggccgccga taaggatgac gttgatttca
      781 tccgcatgca ggggtggcgc cggaaagacc gtcaggggag caaagcagcc atgaaaagag
      841 gaaaaaacgt gcagagcgtg gaagtacgga aacaatcatg gcggggatat ccctgatacg
      901 ttttcggaaa gaagattctg tcttctggag gaattcttct ttgcctgctg ccaggatatt
      961 ttactgttgg tgctcttgtt cccttgattc tttcctttcc ggaaaaaggc attatgcgga
     1021 aggtgcgtgc agggaaacgg attggccggg gtatatttcc ggaatgacgg aaatgctctt
     1081 gatatttttc ccggctgtca gggagcggag catccgtatg agcttgtgaa ccgtttttgt
     1141 ctggttctct tcataatagg tgatcgtggg cagcaggcgt tccatgaagg gttcatacgt
     1201 caggctgacc aggctgagct gatccggaat gctcagcgaa cgggcttcca cccaggaaag
     1261 aatgggaacg atctggttgc cccgtgtggc taccagggcg gacatatccg gatgtttttt
     1321 aaacgctttg gccagatatt caaagaagag cggcgtttca aacggggtgg gtaccaggat
     1381 gggattccat ccctgttccg caaagctgaa aaaacctttt tgcatgagct ggtggccctt
     1441 caacggatca ggaggcaggc acaggcctac cgttcgatgc cccttgttcc aaagatgctg
     1501 cgccgcatga tgcgtggtgg ccgcccagtg gcggtccaga tagggaaggt tgctgctctg
     1561 gtaggaggtg ccgcgcacaa tgcaggggat gcggtgcgtc ttgaaccaga gctgggtttg
     1621 ctccgaggaa cggtgaagta tccagcagac gcattcggat ttggtaacca gcttggcaag
     1681 cctcttgtcc ggattgttgc ctaagatcca gggagcttca aagatacgga cgctgatgcc
     1741 gtcttcctcc agaattttgg agatggtata tatttccgcc aatacgcttt gagccagcct
     1801 tttgaggggc tggggcgtca gaaacccgag aatttctttt ttttcctgaa ccggatggca
     1861 aaagatattt ttaaccgttt cttcttccgt agttttcagg atgcgccgcg gaaccttgat
     1921 gcgtgggggg gcaatccatg catccctttc cagaagggcc agggcctttc tgatggtttc
     1981 cctgcctacg gataacctgt gcccgagttc tctttctccg ggcaggatat tgaccaggct
     2041 gccgctgaga atcatctgtt tgattttatt ggctactgtt tcaaccaatg aagaagctgg
     2101 caacgaagtc atactcttgt atatacgtat cgcattgcgt gaattcgagc caattgtagg
     2161 gattgcctgg cccgcagaat ccgaaaggag atctggtgtt gttgcaagtt gttgatattc
     2221 tgtaaatcgt tttaggattt aagctactgg tctttttttg atgaggtaaa gtgggcggat
     2281 aatgtataaa tgatatagtg ttatcaatgc attacattcc tgagcggtac ggcttcaggt
     2341 ggtttgtaat tatttttaat tcatttaaga taaatatttt attgcgtatt ttgttgggcg
     2401 gagccatttc ttattctcca ttttctcact ttcccccata tttccgccag gaaatgtgca
     2461 cgtatcagca tggatgactt ttctcccgtt tccggcact
//
LOCUS       DAOMSU010000309         2489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4365_length_2489_cov_2.278143, whole genome shotgun sequence.
ACCESSION   DAOMSU010000309 DAOMSU010000000
VERSION     DAOMSU010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4365_length_2489_cov_2.278143"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(252..>333)
                     /locus_tag="PK077_09940"
     CDS             complement(252..>333)
                     /locus_tag="PK077_09940"
                     /inference="COORDINATES: protein motif:HMM:NF005155.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24658.1"
                     /translation="KIAVMVDESDIEEAARVVHKAFNLGA"
     assembly_gap    334..385
                     /estimated_length=52
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<386..1484)
                     /locus_tag="PK077_09945"
     CDS             complement(<386..1484)
                     /locus_tag="PK077_09945"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HRN24659.1"
                     /translation="MALIVQKFGGSSVGTIDRIRNVARRIHETAREGNQVVAVVSAMS
                     GVTDKLIGLARELSETPCERELDVLMATGEQQSIALLCMALHELGEKAMSFTGAQAGI
                     TTFGSHTRGRIHSIDPTLMNKYLQEGNILICAGFQGVTEEGMVQTLGRGGSDLSAIAI
                     AAALKADVCQIFTDVDGVYTCDPRVVKDAKKIQTLSYDEMLEMASNGSKVMQSRSVEF
                     AKKFGVVFEVRNSMNNNPGTIVQEETPSMEAVVIRGISIDRNQARVTITGIPDQIGYT
                     AQILGALAEAEINLDMILANTAHDGYVRQSFTMPSNELGRAQAALKPVMAALGSTVKV
                     ETEAGLAKLSLVGIGMRSHSGVGATAFKALAD"
     gene            complement(1505..1801)
                     /locus_tag="PK077_09950"
     CDS             complement(1505..1801)
                     /locus_tag="PK077_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HRN24660.1"
                     /translation="MHINDTVTPYYVRFQVADQPGVIAEIARILATFGIGISATSSAP
                     SHIDEGGAPWNDLVFILHSCPWGQLQKALEEITRISCVAAEPRVLRIEHLLPQS"
     gene            complement(1934..2464)
                     /locus_tag="PK077_09955"
                     /pseudo
     CDS             complement(1934..2464)
                     /locus_tag="PK077_09955"
                     /inference="COORDINATES: protein motif:HMM:NF012945.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
BASE COUNT          511 a          650 c          750 g          526 t
ORIGIN      
        1 aaaacctggg cggcaagccg gtaaaaccgg aacagggaga gggggcgtca ttcctcctgt
       61 gttcctgtga cggccattcc gcccgcaaca gcatgcggag agagacgccc ttccttcatt
      121 cgggaattgg aaggaaacgg ggggatggcg ttcctcttcc gggaaggggc tgcttttctt
      181 gccgcatgaa aaagccggtt cacgggaacg tgaaccggcg cggaaaaacg agttgataaa
      241 tactgaaaaa atcaggctcc caggttgaac gccttatgca ctacccgggc cgcttcctca
      301 atatcggatt cgtccaccat cacggcaatc ttgnnnnnnn nnnnnnnnnn nnnnnnnnnn
      361 nnnnnnnnnn nnnnnnnnnn nnnnncgtcc gccaaggcct tgaaagcggt ggctcccacg
      421 cctgagtggg aacgcatgcc gatgccgacc agggaaagct tggccagccc cgcttccgtt
      481 tccaccttga cggtggagcc gagggcggcc atgaccggtt taagggcggc ctgggcgcgg
      541 cccagttcgt tggagggcat cgtaaaggac tggcggacat aaccgtcgtg ggcagtattg
      601 gccagaatca tatccaggtt gatttccgct tctgccaggg cacccagtat ctgggccgtg
      661 tagccgattt ggtccggaat gccggtaatg gtgacgcggg cctggttgcg gtcaatggaa
      721 atgccgcgga tgacgacggc ttccatggag ggggtttctt cttgcacgat tgtaccgggg
      781 ttgttgttca tggagttgcg aacttcaaag acgacaccga attttttggc gaattccacg
      841 gaacgcgact gcatcacctt ggacccgttg gaagccattt ccagcatctc gtcatatgaa
      901 agggtttgta tcttcttggc gtctttgacc acgcgggggt cacaggtata gacgccgtcc
      961 acatctgtaa aaatctggca cacatccgct ttcagagcgg ccgcgatggc gatggcggag
     1021 aggtcggaac ctccgcgacc cagcgtctgg accattcctt cttccgtaac cccctgaaag
     1081 ccggcgcaga taaggatgtt gccttcctgc aggtacttgt tcatcagcgt cgggtcaatg
     1141 ctgtggatgc gcccccgcgt gtggctgccg aaggtggtga ttccggcctg cgcccccgta
     1201 aaggacatgg ctttttcacc cagttcatgc agggccatgc agagcagggc gatggactgc
     1261 tgttcgccgg tggccatcag cacgtcaagt tcacgttcgc aaggcgtttc agataattcc
     1321 ctggcaagcc caatcagctt gtccgtcacg ccgctcatgg cggaaacgac ggcgaccacc
     1381 tggttgcctt ccctggcggt ttcatggatg cggcgcgcta cattgcggat gcggtcaatg
     1441 gtgccgacgg agctgccccc gaatttttga acgataagag ccatgtaaca ggatgatatg
     1501 taggttagga ttgaggaaga agatgttcta tgcgcaggac gcggggctcc gccgccacgc
     1561 aggaaatacg ggttatttcc tccagagcct tctggagctg gccccacggg caggaatgga
     1621 ggatgaaaac gaggtcgttc cagggggctc cgccttcatc aatatggctg ggagcggaag
     1681 aggtggcgga aatgccgatg ccgaaggtag ccagaatgcg ggctatttcc gcaatgactc
     1741 ccggctggtc cgctacctgg aagcggacgt aatacggagt gaccgtatca ttgatatgca
     1801 tgatcccgca ggccttggca tagggattga agcccgtatg gtattccgga tagcggcttt
     1861 cgcgcatggc ggtgatgatg tcgctgatga cggcggaagc ggtggggttc ttgcccgctc
     1921 cgcggccgta aaacagcgtt tcccccacaa tgtccccgtt gacggaaatg gcgttgaaca
     1981 cgccgttcac ggaagccagg atatgccagt catggacaaa acagggctgg acacgcagtt
     2041 ccagagcgtc ttcctacccc tcatggtagc ggatgacgac aaggagcttg atggtatagc
     2101 ccagtttttt ggcaaattcg aaatcccggc tggtgatatt ctcaatgcct ctgacgtaaa
     2161 ttttatccgg ggaaatggtt gttccgtagg ccagcatcgc caggatgagc gccttatggg
     2221 cggcgtccca gccgttgacg tccagggagg ggtcttcttc cgcgaagccc agcttctggg
     2281 cctgggccag ggcgtccgcg taatcggccc cgtgttcccc catggcggaa agaatatagt
     2341 tggacgttcc gttaataatg cccacaatgg aattgatgtg gttgcaaatc agggaattct
     2401 gcaagctctg gataatgggg atgccgccgc cggcggacgc ttcgaaataa atgggggtgc
     2461 ccatttccgc ggaaagcttg aaaatctcc
//
LOCUS       DAOMSU010000310         2477 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4394_length_2477_cov_3.215524, whole genome shotgun sequence.
ACCESSION   DAOMSU010000310 DAOMSU010000000
VERSION     DAOMSU010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4394_length_2477_cov_3.215524"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /locus_tag="PK077_09960"
     CDS             complement(<1..1706)
                     /locus_tag="PK077_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRN24661.1"
                     /translation="MSNYTSADVPESSRVVIDPVTRIEGHLRVEMEAGDGVIKNAWTS
                     TTQYRGIEVIACKRDPRDVWAFVERICGVCTGTHAIAALAAVEDALQYPVPVQARLMR
                     DLVSGALGIQDHVIHFYQLQAMDWVDVMSALKADPAETAAIAKSLSDWPLSSAAYFAG
                     VQKKLKDSIAYGQYSIFTNGYWGHPAYKLPPEVNLLGVAHYLQALVWQRDMIKIHTIL
                     GGKNPHPNFLVGGMACAINMNNDQAINQFSLSYLKQLVQTCHDFIHKVYYPDIVAIAG
                     FYKDYAHIGASNPNFFCTGAPSEINTGAPAGKGMIKPGVLLNGDYRNVLPFDQDKIRE
                     FVTSSWYRYTEGRDAGLAPYDGETNADYNGPRPPYKWLSDHPQYTWVKAPRYDGHAMA
                     VGPNARMMIGYAQGVPAIVERVDAACDKLGLPRDNAFMNSTLGRVYGRALDALINVDM
                     MVDQLQEMTDRIRNGETSTFNPEKWEPETWPSSCKGVGWVEAPRGSLSHWVRIENGQT
                     VNYQAVVPSTWNSSGRDAAGQMGPYEYSLAHTGKHPLVDPSRPVEPLRTIHSYDPCQS
                     CAV"
     gene            complement(1733..>2477)
                     /locus_tag="PK077_09965"
     CDS             complement(1733..>2477)
                     /locus_tag="PK077_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HRN24662.1"
                     /translation="TVAGDTVENMLRHCAENASYILAVGSCAYFGSIQAARPNPTGAV
                     GIRDILTDRTVINVPGCPPIGDVITAVIMYILTFDRAPECDLESRPLMAYGTRIHNNC
                     PRRAHFDAGQFVNVFDDENARECFCLYKVGCKGPDTFSPCPIVKWNGGVSFPIQSGHP
                     CIGCTELYFFDRMTPFYKTLPNVNGFGVEASANIIGAVGVAGAGAAIAAHAVGSAIHY
                     RKQHMKEEANVSLPAFGDAPGSPDKDTEE"
BASE COUNT          434 a          725 c          810 g          508 t
ORIGIN      
        1 acggcgcagg attggcacgg gtcatagctg tgaatggtgc gcaggggttc caccgggcgg
       61 gaggggtcca ccagcgggtg tttcccggta tgggccaggg aatattcata tggccccatc
      121 tgccccgccg cgtcccgtcc ggaactgttc catgtactgg ggactacggc ctggtaattc
      181 accgtctggc cgttttcaat ccgtacccag tggctcaggc tgccgcgggg cgcttccacc
      241 caacccacgc ccttgcagga ggagggccag gtttccggtt cccatttttc cgggttgaag
      301 gtggaggttt ccccgttcct gatgcggtcc gtcatttcct ggagctggtc caccatcatg
      361 tccacgttga tcagggcgtc cagcgcacgg ccgtatacgc ggcccagcgt ggagttcatg
      421 aaggcgttgt cgcgcggcag gcccagcttg tcgcaggcgg catccacccg ttccacgatg
      481 gcgggaaccc cctgggcgta gccaatcatc atgcgggcgt tcggacccac ggccatcgcg
      541 tggccgtcat agcgcggggc tttcacccaa gtgtactgcg ggtggtcgga aagccatttg
      601 tagggcggcc tggggccgtt gtagtccgca ttcgtctccc cgtcatacgg agccaggccc
      661 gcgtccctgc cttccgtata cctgtaccag gagctggtga cgaattccct gatcttgtcc
      721 tggtcaaagg gaagcacgtt cctgtaatcc ccattcagaa gcacgccggg cttgatcatg
      781 cctttgccgg cgggggcgcc cgtgttgatt tcagacggag ccccggtgca gaaaaagttg
      841 gggttggacg cgccaatgtg ggcgtagtcc ttataaaatc cggcgatggc tacaatgtcc
      901 ggataatata ctttatgaat gaaatcgtgg caggtttgca ccagctgctt caggtagctc
      961 agggaaaact ggttgatggc ctggtcattg ttcatgttga tggcgcaggc catgccgccg
     1021 accaggaagt tcgggtgcgg gttcttgccg cccaggatgg tgtggatttt gatcatgtcc
     1081 cgctgccaga ccagggcctg gagataatgc gccacgccca gcagattcac ctccggcggc
     1141 agcttgtagg ccggatggcc ccagtagccg ttcgtaaaga tggaatattg cccgtaggcg
     1201 atggagtcct tcagcttctt ctgcactccg gcgaaatacg cggcggagga caggggccag
     1261 tcggaaaggg acttggcgat ggcggcggtt tccgcgggat ccgccttcag ggcggacatc
     1321 acgtccaccc agtccatggc ctggagctga taaaaatgga tgacgtggtc ctggatgccc
     1381 agggctccgc tgaccaggtc tcgcatgagg cgggcctgga cgggcaccgg gtactggagc
     1441 gcgtcttcca ccgcagccag ggccgcgatg gcgtgcgtac ccgtgcagac gccgcagatg
     1501 cgttccacaa aagcccaaac gtcccggggg tcccgcttgc aggcgatcac ctcaatgccg
     1561 cggtactgcg tggtggacgt ccaggcattt ttgatgacgc cgtccccggc ctccatttcc
     1621 acccgcaggt ggccttcgat gcgcgttacc gggtcaatga ccacccggct ggattccgga
     1681 acgtccgctg atgtgtaatt gctcatggta atctggtggt gagggcttct ggctattctt
     1741 cagtgtcctt gtcgggcgag ccgggagcgt ccccgaaggc gggcagggag acgtttgctt
     1801 cctccttcat gtgctgcttc ctgtaatgaa tggcggagcc cacggcatgg gcggcgattg
     1861 ccgctccggc gccggctacg cccacagccc cgatgatgtt ggcggaggct tccacgccga
     1921 agccgttcac gttcggcagc gtcttgtaaa acggagtcat gcggtcaaag aagtacagct
     1981 ccgtacagcc gatgcacggg tggccggact ggatggggaa gctgacgccg ccattccatt
     2041 tgacgatggg gcagggagag aacgtatccg ggcccttgca gcccaccttg tacaggcaga
     2101 agcattcccg cgcgttctcg tcgtcaaaca cgttgacgaa ctgtccagcg tcgaaatggg
     2161 cgcggcgcgg gcaattattg tggatgcggg tgccgtatgc catcagcggg cgcgactcca
     2221 ggtcgcattc cggagcgcgg tcaaatgtaa ggatgtacat gatgacggcc gtaatcacgt
     2281 ctccgatggg cgggcagccg ggcacgttga tgaccgtgcg gtctgtcagg atgtcccgga
     2341 tgcccacggc cccggtgggg ttgggacggg cggcctggat ggagccgaag tacgcgcacg
     2401 agcccacggc caaaatatag gatgcgtttt ccgcgcaatg gcgcagcatg ttctccacgg
     2461 tatctccggc cacggtg
//
LOCUS       DAOMSU010000311         2474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4402_length_2474_cov_3.233981, whole genome shotgun sequence.
ACCESSION   DAOMSU010000311 DAOMSU010000000
VERSION     DAOMSU010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4402_length_2474_cov_3.233981"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="PK077_09970"
     CDS             complement(<1..452)
                     /locus_tag="PK077_09970"
                     /inference="COORDINATES: protein motif:HMM:NF012435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24663.1"
                     /translation="MSSSKAQQDEWSGKIGVILAVAGSAVGLGNFLRFPGLAAQYGGG
                     AFMVAYGLMLVLVGVPVAWAEWSIGRRGGQMGAHCAPGVFWYLTKGSRLWKFLGVLAV
                     LGPSSVAFYYMVVEAWCCGYFWKMLTCPEVFATAEGTAQTFFSFTGMYG"
     gene            588..1211
                     /locus_tag="PK077_09975"
     CDS             588..1211
                     /locus_tag="PK077_09975"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HRN24664.1"
                     /translation="MLLIGKPAPHFSANAVVNGTIVPDFSLDQFKGKKYVILFFYPKD
                     FTFVCPTELIGFQEALGEFDKRDVAVVGCSTDSEFSHWAWVNTPRSQGGIQGVTYPIV
                     SDINKTISADYGVLAGDEEIDEDGNVEVNGELIAYRGLFLIDKDGIVRHQLINDFPLG
                     RSIDEAIRVVDALQHFELYGEVCPLGWHKGDAAMTPSHEGVASYLSK"
     gene            1472..2365
                     /locus_tag="PK077_09980"
     CDS             1472..2365
                     /locus_tag="PK077_09980"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRN24665.1"
                     /translation="MADASSREDIWTKTMDFLNMPDWLKLEMVQSVGVKILHVAVWLV
                     FSWILLKLFCQVLRKITVLKMSPQASRLTVKIVKNVGYIIIGVEAFALMGFDILTLLG
                     AASIIGVAVGFASQTSLSNIISGLFLVGEKQINLGDMIEVNGITGNVDSINLMSVQLR
                     LPNNTMVRIPNEIIIKNPVSNITRFSTRRCDLSLGVDYNCDIEHVVNVLREVVKQNKF
                     CLDDPAPLISFSGFQDSSLGFTVGAWCQKDNFLDCQKTLAHDIKRRFEEEGISFPFPT
                     RSLESRSPIKVEISGPPEKNQ"
BASE COUNT          585 a          766 c          603 g          520 t
ORIGIN      
        1 ccgtacatgc ccgtgaaact gaaaaaagtc tgagccgtcc cctccgccgt ggcaaaaacc
       61 tccggacagg tgagcatctt ccagaaatag ccgcagcacc acgcttccac caccatgtag
      121 taaaaagcca cggatgaagg ccccagaacc gccagtacgc ccagaaactt ccacagcctg
      181 gaccctttgg tcaggtacca gaacacgccg ggggcgcaat gggcgcccat ctggccgccg
      241 cgccgcccga tggaccactc cgcccacgcc acgggcacgc ccaccaaaac aagcatgagg
      301 ccgtaagcca ccatgaaggc gccccctcca tattgggcag ccagccccgg aaagcgcaga
      361 aaatttccca acccgacggc actgccggcc actgcgagaa tcacgcctat ttttccggac
      421 cactcgtctt gttgcgcctt tgaggatgac atggagggaa tactgacaaa aaaagcttca
      481 ttttcaagag aaaacaggct cttttggaac gaatgcggta tcattgcctc ttgactttcg
      541 tccgtttttt ggaatcattc ttttagaacc aaaccaaaaa aacgaccatg cttctcatag
      601 gaaaaccggc acctcatttt agtgcaaacg cagttgtaaa cggtaccatc gtaccggatt
      661 tcagccttga ccagtttaag ggcaaaaagt acgtaatcct gttcttctac ccgaaggatt
      721 ttaccttcgt gtgccctacc gagctgatcg gcttccagga agccctgggc gaattcgaca
      781 agcgtgacgt agccgtcgtc ggctgctcca cggacagcga attctcccac tgggcctggg
      841 tcaacacccc ccgcagccag ggaggcattc agggcgtgac ctaccccatc gtctccgaca
      901 tcaacaagac catttccgcc gactacggcg tgctggccgg agatgaggaa attgacgaag
      961 acggcaatgt ggaagtcaat ggggagctga tcgcctaccg cggcctcttc ctgattgaca
     1021 aggacggcat cgtccgccac cagctcatca acgacttccc gctgggtcgc tccattgatg
     1081 aagccatccg cgtggttgac gccctccagc acttcgaact gtacggggaa gtctgccccc
     1141 tcggctggca caagggagat gcggccatga ccccgtccca cgaaggcgtg gcctcctacc
     1201 tcagcaagta agcagccctc cggcaatatt tcaaggccgc tcccctctcc ggggaacggc
     1261 cttttttcat ccccgctttt tcctgtttcc caaccagatg ccatatccgg ccatacatcc
     1321 gccccccttt cctgcgggat aaatgctgcg tccgcagcgc ctgaaagtcc tgtcctctcc
     1381 gctgccggag gaactgtcat aaaccggggg gaatacgcgc gcgccccttg agccgggcag
     1441 tagcgtttgc tacatccttc atatgaatct catggcagac gcatcctcca gggaagacat
     1501 ctggacaaaa accatggact tcctgaacat gccggactgg ctcaaactgg aaatggtgca
     1561 gtccgtggga gttaaaattc tgcatgtggc ggtttggctg gtgttcagct ggatacttct
     1621 gaaactgttc tgccaggtgc tccggaaaat cactgtattg aaaatgtcgc cccaggcctc
     1681 ccgcctgacg gtaaaaatcg tcaaaaacgt cggctacatt attataggcg tggaagcctt
     1741 tgccctgatg gggttcgata tcctgaccct gctgggcgcg gccagcatca tcggcgtcgc
     1801 cgtaggcttc gcctcccaga cttccctgtc caacatcatc agcggcctct tcctggtagg
     1861 ggaaaagcag atcaacctgg gggatatgat tgaagtgaat ggaatcaccg ggaacgtgga
     1921 ctccatcaac ctgatgtccg tccagctccg gctgccgaac aataccatgg tgcgcatccc
     1981 caatgaaata atcatcaaaa acccggtcag caatattacc cgtttttcca cgcgccggtg
     2041 cgacctgagc ctgggcgtgg actataactg cgacattgag catgtcgtaa acgtcctccg
     2101 ggaagtggtc aaacaaaaca aattctgcct ggacgacccc gcgcccctca tctcgttttc
     2161 agggtttcag gactcttccc tgggctttac cgtaggggcc tggtgccaga aagacaattt
     2221 cctggactgc cagaaaacgc tcgcccatga catcaaacgc cgctttgagg aagaaggcat
     2281 ttccttcccc ttccccaccc gcagcctgga gagcagaagc cccatcaagg tggaaatctc
     2341 cggacctccg gagaaaaatc aataaagaat ctctttttgc tttctttttg ccccgtgaaa
     2401 tttcaatctt gtctgcgtaa aatccgcatc tatgtctgac acatccgcac ccgcctatcg
     2461 aagaatcctc ctga
//
LOCUS       DAOMSU010000312         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4424_length_2464_cov_2.783728, whole genome shotgun sequence.
ACCESSION   DAOMSU010000312 DAOMSU010000000
VERSION     DAOMSU010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4424_length_2464_cov_2.783728"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1049
                     /gene="lepA"
                     /locus_tag="PK077_09985"
     CDS             <1..1049
                     /gene="lepA"
                     /locus_tag="PK077_09985"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRN24666.1"
                     /translation="LEAGDVGYIIANMKSAADVKIGDTYTDYMRPCPSPLPGFKEIRP
                     MVFSGIYPVDSSDFEALKAAMAKLQINDAAFSFQAESSVALGFGFRCGFLGLLHMEII
                     QERLRREFNMDIISTYPSVIYEVTKTNGEEINVDNPSLLPEPQEIQEIREPIVKVFIM
                     LPGEYIGDIMQLVLEKRGSVDNTETIDDMRVMLTCTVPLAEILVDFNDKLKSMTRGYG
                     SMDYEYAGYQAAKLIKMDMLIAGEPVDAFSMIVHQDKAASRGRELAERLKNVIPRQLF
                     TVAIQACIGGKIIARESISPMRKDVTAKCYGGDVTRKRKLLEKQKEGKKRMKAIGKIN
                     IPQEAFIKVLKTGD"
     gene            1410..2393
                     /locus_tag="PK077_09990"
     CDS             1410..2393
                     /locus_tag="PK077_09990"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HRN24667.1"
                     /translation="MSLPRLFMLLLASAALCSCVGTSPEQEEPKVKKSLSEYMQDNKA
                     NSIANVPTGLLSTTPNQQQATHLTATTQEEMTRADSGAVYYTDAHDPDAPIPGLEEAF
                     AQRKENERWIQSYPTALREAQSTGKPILIWFHHSVGSPPSKKLGTELLHTKEFEDWAK
                     RNVVRVCYDQAEKFESEPVYRKRQKMLEYVKKAPSLFGVRGTPVLLVMSPDGSKVDTL
                     RGYYTGQNALYFDQIKNSVKLAKQQYEEFKKTLIPKGYRVWTGVNGNTVFAKLSRYSE
                     KTRTLWLQELDGHQSKTSLKRLSLEDRTWLLEQKEAHENNSWNKRSGPRDA"
BASE COUNT          631 a          706 c          623 g          504 t
ORIGIN      
        1 ccctggaagc cggcgacgta ggctacatca tcgccaacat gaaatccgcg gcggatgtca
       61 aaatcgggga cacgtatacg gattacatgc gcccctgccc atcccccctg cccggtttca
      121 aggaaatccg ccccatggtc ttttccggca tttatccggt ggattcctcc gattttgaag
      181 ccttgaaagc agccatggcg aagcttcaga ttaatgacgc cgctttctcc ttccaggcgg
      241 aatcttccgt agctctgggc ttcgggttcc gttgcggctt ccttggcctg ctccatatgg
      301 aaatcatcca ggagcgcctg cgccgggaat tcaacatgga tatcatctcc acctatccct
      361 ccgtcattta tgaggtcacc aagaccaatg gagaggaaat caatgtggac aatcccagcc
      421 tgctcccgga accgcaggaa attcaggaaa tccgggaacc tatcgtcaag gtgttcatca
      481 tgcttccggg ggaatacatc ggggacatca tgcagcttgt tctggaaaaa cgcggcagtg
      541 tggacaatac ggagaccatt gacgacatgc gcgtgatgct cacctgcacc gtccctctgg
      601 cggaaattct ggtggatttc aacgacaagc tcaaatccat gacgcgaggc tacggctcca
      661 tggattatga atacgccggt tatcaggccg ccaaactcat taaaatggac atgctcatcg
      721 ccggggaacc ggtggacgct ttctccatga tcgttcacca ggacaaggcc gcctcccgcg
      781 gcagggaact ggcggaacgc ctgaaaaacg tcattccccg ccagctcttc accgtcgcca
      841 ttcaggcctg catcggcggc aagattattg cccgggaaag catctccccc atgcgcaagg
      901 acgtgacggc caaatgctac ggcggggacg tcacccgcaa gcgcaaactt ctggaaaaac
      961 agaaggaggg caaaaaacgc atgaaggcca tcggaaaaat caacattccg caggaagcgt
     1021 tcatcaaggt gttgaaaaca ggggattaac ccattttctt tccgcaggac cgctaaacgg
     1081 gtgctttcct ttttcctccc ggttttatat cagttctccg ttgaatatgg agagactgtt
     1141 ttcccgggtt ctctcgacct gtgttttggc gagcatacat gcccctgcca acggagagga
     1201 tgaggtcagg atggatattt acgagcagac ctttcttttt gacggcaaaa caactggaaa
     1261 cgtcacgcgc gtggtaacac ccgtcagcaa atcatcgcca tacggaaccg gaagcagaag
     1321 gaatgggaaa ccaagaaaca ctgaaaccgc cgcagtcgtt gaacaattcc cggttgcaca
     1381 tttttccctg ttatgctatc aggtcagtca tgagtctgcc caggctgttc atgcttcttc
     1441 ttgcctccgc cgccctctgc tcctgcgtag ggacctctcc ggaacaggaa gaaccgaagg
     1501 taaaaaaaag cctttcggaa tacatgcagg acaataaggc caattccatc gccaatgttc
     1561 cgacggggct gttgagcact actccgaatc agcaacaggc cacccatctt accgccacca
     1621 cacaggagga aatgaccagg gcagacagcg gggccgtcta ttacacggac gctcatgacc
     1681 cggacgcccc catccccggg ctggaagaag ctttcgccca gcgtaaggaa aacgaacgct
     1741 ggatccaaag ctatcccacc gccctccggg aagcccagag taccggaaaa cccattctca
     1801 tctggttcca ccattccgtg ggcagccctc ccagtaaaaa acttgggacc gaactcctcc
     1861 atacgaagga atttgaagac tgggcaaaaa ggaatgtcgt ccgcgtctgt tacgaccagg
     1921 cggaaaaatt tgaaagcgaa cctgtttaca ggaagcgcca gaagatgctg gagtatgtga
     1981 aaaaggctcc ctccctgttt ggcgtgagag gaacgcccgt gcttctggtc atgtctccgg
     2041 acggctccaa agtggatacg ctgcgcggtt actacaccgg acaaaacgcc ctttattttg
     2101 accagatcaa gaacagcgtc aagctggcga agcaacaata tgaagagttt aaaaaaacgc
     2161 tgattcccaa aggctaccgg gtctggacag gagtcaatgg caataccgtc ttcgccaagc
     2221 tttcccgcta ttcggaaaag acccggaccc tctggctcca ggagctggac gggcaccaga
     2281 gcaaaacatc cctaaagagg ctcagccttg aagacagaac atggcttctg gagcagaaag
     2341 aagcccatga aaacaacagc tggaacaagc gatccggccc acgtgacgcc tgaacggctt
     2401 ttccccggct gtccggctgc ggaacggttt attcatgggc ttccgctcca tccccatttt
     2461 tgta
//
LOCUS       DAOMSU010000313         2464 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4425_length_2464_cov_2.685347, whole genome shotgun sequence.
ACCESSION   DAOMSU010000313 DAOMSU010000000
VERSION     DAOMSU010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4425_length_2464_cov_2.685347"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..168)
                     /gene="purH"
                     /locus_tag="PK077_09995"
     CDS             complement(<1..168)
                     /gene="purH"
                     /locus_tag="PK077_09995"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: protein motif:HMM:NF014225.2"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRN24668.1"
                     /translation="MAIQRALISVSDKTGLEEFAKGLHEFGVELISTGGTAAFLKGLG
                     LPVIEISDYTGE"
     gene            406..861
                     /gene="rplI"
                     /locus_tag="PK077_10000"
     CDS             406..861
                     /gene="rplI"
                     /locus_tag="PK077_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRN24669.1"
                     /translation="MATMEVILATKIEGLGAEADLVTVKAGYGRNYLIPKGLAHEATA
                     SNRRFIANLQAARAKREAEELSAAQEVAAKISGLTVDLTLEVGQGGKAFGAITNQNIH
                     DALTAQGVEVDRRAIELEKPIKSEGEHEVVIKVHPQVEATLKVVVKENA"
     gene            1030..2022
                     /locus_tag="PK077_10005"
     CDS             1030..2022
                     /locus_tag="PK077_10005"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRN24670.1"
                     /translation="MIRLYRQTPEGIERTTQVDAAEQHLDSVFWIDLLTPEPAEIKFV
                     ERLCGLEMPTYDEMREIEATSRLYTEDGARFMTTTVLSRVDTESPSLSEITFVLMGAK
                     IITIRHSDSYSFRVFSHQLLRQKQISRDQVFTGLLETIVDRQADVLERFGAELDRLSK
                     NIFRRDEPEGKTSKRVPVSSALRLTLQDLGRVGDLLTRQRDCLVNLLRLLTYASNEEA
                     LDDTNSTLYIKLRPLSRDVTSLSEYANFLSSNVNFMLDAVLGLINIEQNEIVKIFTVA
                     AVVFMPPTLIASIYGMNFSHMPGLENEYGYYISLVVMLVSIILPLVYFRSRRLL"
     gene            2085..>2464
                     /gene="argB"
                     /locus_tag="PK077_10010"
     CDS             2085..>2464
                     /gene="argB"
                     /locus_tag="PK077_10010"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HRN24671.1"
                     /translation="MDSKITRLIEQASVIVGALPYLQAYRDKTFLIKFGGSAMDDARL
                     VKKLMRDIVLLEVLGFNPVIVHGGGKAISKAMAEAGLEARFVNGLRVTTPEAISIVER
                     TLSGTINPGLVQMFRDYGGKGVGIP"
BASE COUNT          548 a          798 c          615 g          503 t
ORIGIN      
        1 ctctccggta tagtcggaaa tttcaatcac cggaaggccc aggcctttca ggaaagcggc
       61 ggtcccgcca gtggaaataa gctctactcc aaactcgtgc aggcccttgg caaactcctc
      121 caggccggtc ttgtcggaaa cggatatcaa tgcgcgctga atagccataa gataaatatc
      181 tgtaatgtac ccgcggcgcc ccctccggga gggaacgcga taaatgcggt tcctttccct
      241 acatactact gccgcgtgaa gcaagcgcaa tgtcaccaat catgacaacc gggaggattt
      301 ttcctcccaa aacataaaaa aacgcccttt ttatcgctct tgtcttgaca ttcgggcgaa
      361 tacttactac ttcaaccgct cacaaataac catttctttt tagccatggc aacgatggaa
      421 gtaattctcg caacaaaaat tgaagggctc ggcgcagaag ccgacctggt gaccgttaag
      481 gctggctatg gccgcaacta cctcattccc aagggtctgg cccacgaagc aacggcctcc
      541 aaccgccgtt tcatcgccaa tcttcaggcc gcccgcgcca agcgcgaagc ggaagaactc
      601 agcgccgctc aggaagtggc cgccaagatc agcggactga ccgtagacct cacccttgaa
      661 gtgggccagg gcggcaaggc gttcggcgcc atcaccaacc agaacattca cgatgccctg
      721 accgctcagg gcgtggaagt ggaccgccgg gcgattgaac ttgaaaagcc gatcaagagc
      781 gaaggcgaac acgaagtcgt catcaaggtg cacccccagg tggaagccac gctcaaggtg
      841 gtcgtcaagg aaaacgccta agaggcgcgc atcccttttc cggcaggccc ggttcccacc
      901 ttgggaaccg ggcctctttt tgtcaccctt tttacttccc ttccctgtac aaacaaggca
      961 tcatgcctgc gttgggcgtc actcgacctc atgcgcggca tcgttccatc cccctcctaa
     1021 atcaacggca tgatccggct ctatcgtcaa accccggaag gcattgagcg caccacccag
     1081 gtggatgcgg cggaacagca cttggattcc gtcttctgga ttgacctcct taccccggaa
     1141 ccggcggaaa tcaagtttgt ggaacgcctc tgcgggctgg aaatgccgac ctatgacgag
     1201 atgcgggaga ttgaggccac cagccgtctg tacacggaag acggggcgcg cttcatgacc
     1261 actaccgttc tcagccgggt agatacggaa tccccctccc tgtcggaaat cacctttgtc
     1321 ctcatggggg ctaaaatcat caccatccgc cattcggact cctattcctt ccgcgttttt
     1381 tcccaccagc tcctgcgcca gaagcagatc agccgggacc aggttttcac cggcctgctg
     1441 gaaaccatcg tggaccgcca ggcggacgtg ctggaacgtt tcggcgcgga actggaccgc
     1501 ctttccaaaa acatcttccg cagggacgaa cctgaaggca agaccagcaa gcgggtgccc
     1561 gtctccagcg ccctgcgcct gacgctccag gacctgggcc gcgtgggcga cctgctcacc
     1621 cgccagcggg actgcctggt caaccttctg cgcctgctca cgtacgctag caatgaagag
     1681 gctctggacg acaccaactc cacgctgtac atcaagctgc gccctctgag ccgtgacgtc
     1741 acctcccttt ccgaatacgc caactttctt tccagcaacg tcaacttcat gctggacgcc
     1801 gttctgggac tcatcaacat cgaacagaac gaaatcgtta aaatcttcac cgtggcggcc
     1861 gtggtcttca tgcctcccac tctgatcgcc agcatttacg ggatgaactt ctcccacatg
     1921 cccggcctgg aaaacgaata cggctactac atttccttgg tggtcatgct cgtttccatc
     1981 attctccccc tggtttactt cagaagcagg cgcctgcttt gaacgggccc tgcctcagaa
     2041 tctccctctc cttccgttaa aactcctacc gtttcattac tgacatggac tccaaaatca
     2101 cccgcctgat cgaacaggcc tccgtcattg tcggcgccct cccctatctc caggcctacc
     2161 gggacaaaac attcctcatt aaattcggcg gcagcgccat ggatgacgcc cgcctggtca
     2221 agaaactcat gcgggacatc gttctgctgg aagtcctggg cttcaacccc gtgatcgtcc
     2281 acggcggagg caaagccatt tccaaggcga tggcggaggc ggggctggaa gcccgtttcg
     2341 tcaacgggct tcgcgtcacg actccggaag ccatttccat cgtggaacgc accctctccg
     2401 gcaccatcaa tcccggcctg gttcaaatgt tccgcgacta cggcggcaag ggcgtaggca
     2461 ttcc
//
LOCUS       DAOMSU010000314         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4461_length_2449_cov_2.658730, whole genome shotgun sequence.
ACCESSION   DAOMSU010000314 DAOMSU010000000
VERSION     DAOMSU010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4461_length_2449_cov_2.658730"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PK077_10015"
     CDS             complement(<1..905)
                     /locus_tag="PK077_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRN24672.1"
                     /translation="MIIPEEPYLGFAAFCHIAGAFCLIPALLHTRTPQGTIAWLISLL
                     AFPYIAVPFYLILGRRRFSGYVETRRRQTDPESAWGELTDKITNCMVPYAVQSSDTAG
                     KIMRTLSNIVRLPVCRGNSCRLLIDAANAFPRIYDAIKNAEHYILIEFFIIKNDSVGQ
                     NLKELLIERARAGIRIYMLYDEIGSHKLPPGYISALRKAGVNIEPFNGKRHFLSNILR
                     LNFRNHRKLVVVDGSTAFIGGMNIGREYLGKGALGYWRDTFVQLEGPSVQQTQISFLE
                     DWNWAMLKCGPSSLPRLRWEITPQP"
     gene            1050..1865
                     /locus_tag="PK077_10020"
     CDS             1050..1865
                     /locus_tag="PK077_10020"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRN24673.1"
                     /translation="MKQYLALLEDVLTNGAGREDRTGTGTIGVFGRQSRYDLRDGFPC
                     LTTKKLHLRSIIYELLWFLKGETNIRFLKDNGVSIWDEWADENGDLGPVYGAQWRCWP
                     GNDGKPIDQIARLIDGLKHNPWSRRHIVSAWNVALVDDMALPPCHSLFQFCVIPAQPG
                     EEKHGLSLQLYQRSADLFLGVPFNIASYALLLLMTAQVCGYEAREFIHTFGDLHLYRN
                     HLDQAREQLSRTPRPLPVMKLNPEVKTIDGFHYEDFELVGYDPLPHIKAPISV"
     gene            1888..2367
                     /locus_tag="PK077_10025"
     CDS             1888..2367
                     /locus_tag="PK077_10025"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HRN24674.1"
                     /translation="MSQPVTYTGVVAMAPDRGIGYRGALPWHLPDDLKTFKRITTGHP
                     VLMGRKTYESIGRPLPGRQNIVLTRDPAWTAEGVQVVHSAGELECLELMDPEIMVIGG
                     AEIFSLMMPSMSRMWVSKVKGEYPADTFLPPFENRLERAVLKEHFEDFDLYLYEQVK"
BASE COUNT          533 a          632 c          748 g          536 t
ORIGIN      
        1 tccggctggg gcgtgatttc ccagcggagg cggggaaggg aggaggggcc gcatttcagc
       61 atggcccagt tccagtcttc cagaaagctg atttgcgttt gctggacgga aggcccttca
      121 agctgaacaa acgtgtcgcg ccagtagccc agagcgccct tgcccaggta ttccctgcct
      181 atattcatgc ctccgatgaa ggcggtggag ccgtccacca ccaccagttt ccggtggttg
      241 cggaagttca gccgcaggat gttgctcagg aaatggcgct tcccgttgaa aggttcaatg
      301 ttcactcctg ctttccgcag ggcggaaatg taaccggggg ggagcttgtg ggagcctatt
      361 tcatcataca gcatgtaaat gcggatgccg gccctggccc gttcaatcag cagctccttc
      421 aggttctgcc ccacggagtc gtttttgatg atgaagaatt caatcaggat gtaatgctct
      481 gcgttcttga tggcgtcgta gatgcgcggg aaagcgttgg ccgcgtcaat aagcaggcgg
      541 caggaattgc cgcggcagac gggcagccgc acgatattgg aaagcgtgcg catgattttt
      601 cccgccgtgt ctgatgactg tacggcatac ggaaccatgc agtttgtaat tttatccgtc
      661 agttcccccc atgcggattc ggggtccgtc tggcggcggc gcgtttccac atatccgctg
      721 aatctccggc gtcccaggat caggtaaaag gggacggcaa tatatggaaa tgccagcagg
      781 gaaatgagcc acgcgattgt tccctgaggc gtacgcgtat ggagcagggc cggaatcagg
      841 cagaaggcgc ctgcaatgtg gcaaaaggcg gcaaatccca aatatggctc ttcaggaatg
      901 atcatggaac ggaaaaccgg aaaagatgcg ggggatgaaa acggcgggga gaagtgaaaa
      961 ggctggcttt tggagcagga gatgatacta aggtggcgca tgctcgtatt atcacgggtc
     1021 cagggattag aaaagcgtaa tttaagggaa tgaagcagta tttggcactt ttggaggatg
     1081 ttttgaccaa tggggcgggg cgggaggacc gtaccggaac gggcaccatc ggcgtgttcg
     1141 ggcggcagag caggtatgac ctgcgcgacg gttttccgtg cctgacgacc aagaagcttc
     1201 atttgcgctc catcatttat gagctgctct ggttcctgaa aggggagacc aatatcaggt
     1261 ttttgaaaga caacggcgtc agcatctggg atgaatgggc cgacgaaaac ggggatctgg
     1321 ggcccgtgta cggcgcgcag tggcgctgct ggccgggaaa cgacggaaaa cccattgacc
     1381 agattgccag actgattgac ggcctgaaac ataatccctg gtcccggagg cacatcgtca
     1441 gtgcgtggaa tgtggccctg gtggatgaca tggccctccc tccgtgccat tccctgttcc
     1501 agttttgcgt gattcccgcc cagccgggag aagaaaagca cggcctttcc ctccagcttt
     1561 accagcgcag cgcggatctt tttctggggg ttcccttcaa tatagcttcc tatgcgctgc
     1621 tgctgcttat gaccgcccag gtatgcgggt atgaagcccg ggaattcatc catacgttcg
     1681 gggatctgca cctgtaccgg aatcatctgg accaggcgag ggaacaactc tcccgcacgc
     1741 cccgtcctct tccggtcatg aagctcaacc cggaggtaaa aaccattgac ggattccatt
     1801 atgaggattt tgagctcgtg gggtatgatc ccctgccgca catcaaggct cccatctccg
     1861 tttagaacat tcatccctta atccatcatg tcacaacccg ttacttatac aggagtggtc
     1921 gccatggctc ccgaccgcgg cattggttac cggggggcgc tcccctggca tttgccggac
     1981 gacctcaaga cgttcaagcg cattacgacg gggcaccccg tgctgatggg ccggaaaacg
     2041 tatgagagca taggcaggcc tcttcccggc aggcagaaca tcgtgctgac ccgtgacccg
     2101 gcgtggacgg cggaaggcgt tcaggtggtt cattccgcag gggagttgga atgtctggaa
     2161 ttgatggatc cggaaatcat ggttatcgga ggggcggaga ttttttccct gatgatgccg
     2221 tccatgtccc gcatgtgggt ttccaaagta aagggggaat atccggcgga cacttttctt
     2281 cctccgtttg agaaccggct ggagcgtgcc gtcctgaagg aacattttga agattttgac
     2341 ctgtatttgt acgagcaggt caaatagccg ctcttgtccg taactccgcg ggaggcgctg
     2401 aagcataaaa aagacggagc ggccagggcc gccccgtcct tcatggagc
//
LOCUS       DAOMSU010000315         2449 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4462_length_2449_cov_2.236424, whole genome shotgun sequence.
ACCESSION   DAOMSU010000315 DAOMSU010000000
VERSION     DAOMSU010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4462_length_2449_cov_2.236424"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>2449
                     /locus_tag="PK077_10030"
     CDS             <1..>2449
                     /locus_tag="PK077_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HRN24675.1"
                     /translation="LSGKAPLLFNYFKQLFAQVTNPPIDPIREELVMSLTTYIGNHPN
                     ILEETPEHARLIKMARPVITDEELNRLCNIREAGFPSARLPLQFPEGGDGKALRETLE
                     SLAESAVGLVRSGVRILVLTDRNIGNGYLPVPSLLACSVVNRALASAGLRSDVGLILE
                     TGEARETMHFALLLGFGATAVNPYLALATVTSLAAPQDCPLDVVKASGNYINAIDKGL
                     LKIMSKMGISTLRSYRSSQLFEAVGLNRELIDEFFPGTVSRVGGIGLDEIAAECNQRA
                     AQHAEHGDKLDAGGQYKYKKGGENHLWNPQTLQAFRAAVRDNDERKYREFADYSNRQA
                     QHLCTLRGLFEFAPADAIPLEEVESVDSILRRFVSGAMSLGSLSPEAHETIAIAMNKI
                     GAKSNSGEGGEDEARYEPNARGEVRYSAIKQVASGRFGVTINYLRHASELQIKMAQGA
                     KPGEGGQLPAHKVDPYIARLRHSMPNVSLISPPPHHDIYSIEDLAQLIYDLRNSNPDA
                     RVSVKLVSEVGIGAVAAGVVKAHADVVVVSGHDGGTGASPLTSVKHAGLPWELGLAET
                     QQTLVLNKLRSRVRLQVDGQLRTGRDVVMAALLGAEEFGFGTAILISIGCAMLRRCHE
                     NTCPVGVATQDPALRAKFSGKPEYVINYLRFVARETREILASLGLRSLDEAVGRTDLL
                     SVNRAIDFYKARHLDFSSILHAAEGDGRRFDPSFEKEPLDNYDRRHLLPALKPLLEKG
                     EKMELCRDVRNVDRTVGTELSGELVMKFGPRGLAEDTLTVHFTGCAGQSFGAFLAPGV
                     TFELVGEANDFVGKGLSG"
BASE COUNT          483 a          719 c          761 g          486 t
ORIGIN      
        1 cctctccgga aaggctcccc tcctgttcaa ttatttcaag cagctcttcg cccaggtgac
       61 caaccctccc atcgacccca tccgggagga gctggtcatg agcctgacca catacatagg
      121 caaccatccc aacattctgg aagaaacgcc ggagcatgcc cgtcttatca agatggcgcg
      181 tcccgtcatt acggatgaag agctcaaccg cctctgcaat attcgggagg ccggctttcc
      241 ctccgccagg cttcccctcc agttcccgga aggcggggac gggaaagccc tgcgggagac
      301 gctggaaagc ctggcggaat ccgccgtggg cctggtcagg tccggcgtgc gcattctggt
      361 gttgacggac cgcaacatag gaaacggcta ccttcccgtt cccagtcttc tggcctgttc
      421 cgtggtgaat cgcgcgctgg cttccgccgg gctgcgttcc gacgtggggc tgattctgga
      481 aaccggggag gcgagggaaa caatgcattt tgcgcttctg ctgggcttcg gagccacggc
      541 ggtcaatccg tacctggctc tggcgacggt tacttccctg gccgcgccgc aggactgccc
      601 gctggatgta gtgaaagcct ccggaaacta catcaatgcc atagacaagg gattgctcaa
      661 aatcatgtcc aagatgggca tttccacgtt gcgcagctac cgatcctccc agctgttcga
      721 ggctgtgggg ctgaaccggg aactgattga cgaattcttt cccggcaccg tcagccgcgt
      781 aggcggcata gggctggatg aaatagccgc ggaatgcaac cagcgcgccg ctcaacacgc
      841 ggaacacggg gacaagctgg atgcgggagg gcagtacaag tataaaaaag gcggggagaa
      901 ccatctctgg aatccgcaga cgcttcaggc tttccgcgct gccgtgcgtg acaatgacga
      961 acggaaatac cgggaattcg cggattacag caatcggcag gcgcagcatc tctgcaccct
     1021 gcgcggcctg tttgaatttg ctccggcgga cgctatcccc ctggaagaag tggaaagcgt
     1081 ggattccatc ttgcgccgtt tcgtttccgg ggccatgtct ttggggtccc tcagtccgga
     1141 agcccatgaa accatcgcca ttgcgatgaa caaaatagga gccaaaagca attccgggga
     1201 agggggggaa gatgaagccc gctatgaacc caacgcccgg ggcgaagttc gctacagcgc
     1261 catcaagcag gtggcgagcg ggcgttttgg cgtgactatt aattatctgc gccacgcttc
     1321 cgagctccag attaagatgg cccagggggc caagcccggg gaaggggggc agctcccggc
     1381 gcacaaggtg gatccgtaca tcgcccggct gcgccattcc atgccgaacg tcagcctcat
     1441 ttctcctcct ccgcaccatg acatttactc cattgaagac ctggcccagt taatttacga
     1501 cctgcgcaac tccaatccgg atgcccgtgt ctccgtcaag cttgtttccg aggtgggcat
     1561 cggcgcggtt gccgcgggtg tggtgaaggc ccatgcggat gtggttgtgg tcagcggcca
     1621 cgacggcgga acgggcgcct ctcctctcac cagcgtgaag cacgccgggc tgccgtggga
     1681 gctggggctg gcggaaacgc agcagacgct tgtgcttaac aagctccgtt cccgcgtccg
     1741 tctccaggtg gacggccagc tgcgcaccgg gagggacgtc gtgatggccg ccctgctggg
     1801 ggcggaggaa ttcggttttg gaaccgccat tttgatctcc atcggctgcg ccatgctccg
     1861 caggtgccat gaaaatacgt gtccggtagg cgtcgctacg caggatccgg ccctgagggc
     1921 caaattcagc ggcaagccgg aatacgtcat caactacctc cgctttgtgg cccgggaaac
     1981 gcgggaaatc ctggcttccc tggggcttcg ttccctggat gaagccgtag gcaggacgga
     2041 tctgctttcc gtcaaccgcg ccatagattt ttacaaggcc cggcatttgg atttctcctc
     2101 catcctgcat gcggcagagg gggatggacg caggtttgac ccctcttttg aaaaggaacc
     2161 gctggacaac tacgaccgcc gccatttgct gccggcattg aagcccctgc tggaaaaggg
     2221 ggaaaagatg gaactctgcc gcgacgtccg caatgtggac cggacggtgg ggacggaact
     2281 ttccggtgag ctggtgatga aattcgggcc gcgcggcctg gcggaagata cgcttacggt
     2341 gcactttacc ggatgcgcgg ggcaaagctt cggggctttt ctggctcccg gtgttacctt
     2401 tgaacttgtc ggggaggcga atgactttgt cgggaagggc ttgtctggc
//
LOCUS       DAOMSU010000316         2444 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4471_length_2444_cov_3.095856, whole genome shotgun sequence.
ACCESSION   DAOMSU010000316 DAOMSU010000000
VERSION     DAOMSU010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4471_length_2444_cov_3.095856"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1616
                     /locus_tag="PK077_10035"
     CDS             <1..1616
                     /locus_tag="PK077_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M60 family metallopeptidase"
                     /protein_id="HRN24676.1"
                     /translation="GAYEDKEFRIASYKAYSHPEFAAKVRNINALNKFDNPTGIVAEK
                     GDEILVFVGPTHGEDIGLASVSPAGIESSSYPLNEGVNKIRINRSGLLYVMYHTDIST
                     PKKPITVHIPVGSGIVNGYFDVTRHTDKDWKRMISNAPHSMFDIVGRNSMMILHTKYL
                     KDYSPDSITKSVRVWDESVKAMWKIMGFDKYPQPHNNRQLGVSVEGGAHMFATWYYCG
                     YSIGDQGNTLKNEVLAPGVLQGNRLWGIGHEIGHCYQHPFNWRSMSESSNNFFAQLIL
                     DQVTNAINGNEQASDMENPCKYLLSEAVKGMPFHDLNGWAKWGFAQYSFYLYFHKLGI
                     NPEFYPRLFESLRRKPLSRQAYEVSEAHLALYERICNISRADFTDDFEIFNWFVPIDR
                     KGHQYGDYSFKMTEEMARASKARIAAKRYPKPKFRIAFLHQHGKTVNLWGQNLHGAQL
                     NGYWTKYKQNAKLSPSVSASKKDNMIIVRNGENAAAFCVVTNGKVVGYYDRQKFDVSG
                     VEWNDTSKVYAIPIQTAEPYKLIYAAGRS"
     gene            complement(1689..>2444)
                     /locus_tag="PK077_10040"
     CDS             complement(1689..>2444)
                     /locus_tag="PK077_10040"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRN24677.1"
                     /translation="DDEISRICVDVLRTEKDRHRRPGTPRNGGNHFIEIAQGTEDAFL
                     CIHSGSRNFGLKIAERYQKIAVDTCPDAYLKERKKGLCYLQGENTLNYMRDMKVAQRF
                     AALNRRVILHELLDDAADLESFDTVHNYIAEDQIIRKGAVRAGRGEKLIVPLNMRDGS
                     VICIGKGNEEFNSSSPHGAGRSMSRKKAKANLSLEEFEASMQGIFSTCVSRATLDEAP
                     MAYKDGESVLDNIHETAEIVERIRPVYNFKAPE"
BASE COUNT          592 a          582 c          657 g          613 t
ORIGIN      
        1 ccggcgccta tgaagacaag gaattccgca ttgcgtccta caaggcttac agccatccgg
       61 agtttgctgc gaaggtgaga aatatcaacg ccctgaacaa atttgacaat cctaccggaa
      121 ttgtcgcgga gaaaggagat gaaattcttg tgttcgtagg acctacccat ggagaagata
      181 ttgggctggc ctccgtttct ccagcgggaa tagagtcttc ctcctatcct ttgaatgaag
      241 gggtaaacaa aattagaatc aaccgttcgg gattgcttta cgtgatgtat cacaccgata
      301 tcagcacgcc caagaagccg ataacggtgc atattcctgt gggaagcggc atagtgaatg
      361 gatattttga cgtgacgagg cacacggaca aggactggaa gcgtatgatc agcaacgccc
      421 cgcatagcat gttcgatatc gttgggcgga attccatgat gattcttcac acaaagtacc
      481 tgaaagatta cagtccggat tccattacga aatccgtcag ggtatgggat gaaagcgtca
      541 aggccatgtg gaaaatcatg ggatttgaca agtatcccca gccgcacaat aaccggcagc
      601 ttggcgtttc cgtagaagga ggagcccata tgtttgcaac ctggtattac tgcggttaca
      661 gtattggaga tcagggaaat acgctgaaaa atgaagtgct cgcgcctgga gttcttcagg
      721 gaaaccgcct ttggggaatc gggcatgaaa tcggtcattg ctaccagcat ccgttcaact
      781 ggcgcagcat gtcggaaagt tccaacaatt tttttgccca gctgattctg gaccaggtga
      841 ccaatgccat taatggcaat gagcaggcct ccgatatgga aaacccgtgc aaatacctgc
      901 tttcagaagc ggtaaagggc atgccgttcc atgatttgaa cggctgggcc aaatggggtt
      961 ttgctcaata ttccttctat ctgtatttcc ataagctggg aatcaatccc gaattttatc
     1021 ctaggctgtt tgaatcattg cggcgcaagc cgctttccag gcaggcgtat gaggtttccg
     1081 aagcccattt ggccttgtat gaacgcattt gcaatatcag cagggcggac tttaccgatg
     1141 actttgaaat ttttaattgg tttgtcccga ttgaccgcaa ggggcatcaa tatggggatt
     1201 acagttttaa aatgacagag gaaatggccc gggcatccaa ggcccgcatt gccgccaaac
     1261 ggtaccccaa accgaaattc cgcattgcct tcctgcatca gcatggaaag acggtcaatt
     1321 tgtgggggca gaatctgcat ggggcccagc tcaacggcta ttggaccaaa tacaagcaga
     1381 acgcaaaact cagcccgtct gtaagcgcct ccaaaaagga taacatgatt attgtgcgga
     1441 atggggaaaa tgcagccgct ttctgtgtag tgaccaatgg aaaggtggta ggttattatg
     1501 accgtcagaa gtttgacgtt tccggcgtgg aatggaatga tacctctaaa gtgtacgcca
     1561 tcccgattca gacggcggaa ccttataagt tgatttatgc tgccgggcgt tcctgaccgt
     1621 gttttggagg tttgggaaaa gcctcccgga aaatcatatt ccgggagggg cgggtattgg
     1681 gacgcctgct attcgggtgc cttgaagtta tatacgggcc tgatgcgttc cacgatttcc
     1741 gccgtttcat ggatgttgtc cagcacgctc tcgccatcct tgtaggccat tggcgcttca
     1801 tccagcgtag cgcgggaaac gcaggtggag aaaataccct gcatggaagc ctcgaattcc
     1861 tccagggaaa gattggcttt agcctttttg cgggacatgc tgcggcctgc gccgtgcgga
     1921 gatgaggaat tgaattcttc atttcctttg ccgatgcaga tgacggagcc gtcacgcata
     1981 ttcaggggaa cgatgagttt ttctccgcgt ccggcccgga cggctccctt gcggatgatt
     2041 tggtcttccg caatgtagtt gtgcacggtg tcgaaacttt ccagatcagc tgcatcatcc
     2101 agcagttcat gcagtatcac gcggcggttc agggctgcga agcgctgagc caccttcata
     2161 tcccgcatgt agtttagcgt attttctccc tgaaggtagc agagtccttt cttgcgttct
     2221 ttaagatagg cgtccggaca agtatccacg gcgattttct ggtagcgttc cgcaatcttc
     2281 agaccgaaat tgcgggaacc ggagtggatg cacagaaagg cgtcttccgt tccctgtgct
     2341 atttcaatga agtggttgcc gccgttccgt ggggtccctg ggcggcggtg cctgtctttc
     2401 tccgttctca gcacatccac gcagatgcgg gagatttcat catc
//
LOCUS       DAOMSU010000317         2443 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4478_length_2443_cov_2.180905, whole genome shotgun sequence.
ACCESSION   DAOMSU010000317 DAOMSU010000000
VERSION     DAOMSU010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4478_length_2443_cov_2.180905"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1880)
                     /locus_tag="PK077_10045"
     CDS             complement(90..1880)
                     /locus_tag="PK077_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HRN24678.1"
                     /translation="MTDKKNQPAEKTRLSWSWLRMALNLREKVKLGDKQVIFIWAVVV
                     GALGALMALVFEMGVELVQDVLTGRTSYRQIQVFQEMAGQDPAWCIFVPAAGGLLAGL
                     TLLFTHRFVPAKATEYMEAVALGNGYVPPKPSLLRSLSAVFSVGSGASIGREGPLVQA
                     AAVAGSALGRFFHLSAPRLRLVVACAAASGMSSAFHTPLAGGLFVSEIVLGALTIDFL
                     APLLVASCAGYFTMGLFHEPAPIYQLQQEVSLAGNQHVLWCVLLGALASLVASFWLLI
                     LKKSRRYLNGKRQWLPVRLTAAGVLVGAIAIFYPEIVGNGKNIITSLIHYEFDAARAA
                     VLLFLKIFTVAIVFGVGTVGGALTPSLTIGSVCGFLFSAALTQLGVPGDHAIAYSLVG
                     MAAFFTTAANAPITSLVLVVEFTMAGQMMFPLIIGVLVSYGMARLTKAQSMYHDSLAF
                     GPRSTFDKPLAQVQLQDVARKDPPVVHPLDKFGTIAAMLIKNPAQPIFVTSPVGKYLG
                     SVVAEDVAAFARNKELAQAVLAMDVLRSDMPTLPADMHLPEALGIFSRPHCAESLALV
                     NPNNDLLLGVVNKTDLYLVLSEIMRREKLQ"
     gene            complement(1877..2350)
                     /locus_tag="PK077_10050"
     CDS             complement(1877..2350)
                     /locus_tag="PK077_10050"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HRN24679.1"
                     /translation="MENQKPSEDPVADNLQIHVRTFQPSTTQAQALETLTDSMAEVLD
                     DRQLRHIKNAVLLREETQTTYLDHGLAVPHGRTSALDSMQVTVGISPEGVLWPDDSRN
                     AHLIVMLGVPAAMVTGYLTTMQKLLRWHKNAPLGPNGEWTGDEASLLASLQQALQ"
BASE COUNT          497 a          679 c          805 g          462 t
ORIGIN      
        1 ggtctttgag agcttgcccg tataggcatc gccgcctttt accacgattt atggttgatc
       61 acattgccct ttgggcggca aggcagattt tattgcagtt tttccctgcg catgatttca
      121 gagagcacca ggtagaggtc cgtcttgttg actacgccca gcagcagatc gttgttcggg
      181 ttcacaaggg ccagggattc cgcgcagtgg gggcgggaga aaatgcccag ggcttccggg
      241 aggtgcatgt ccgcaggaag cgtaggcatg tcgctgcgca gcacatccat ggccagtacg
      301 gcctgggcca gctccttgtt gcgggcaaag gccgccacgt cttccgccac cacgcttccc
      361 aggtatttgc cgaccgggga agtgacgaag atgggctggg ccgggttttt aatgagcatg
      421 gcggcaatgg tgccgaattt gtccagggga tgcacaacgg gcggatcctt gcgcgctaca
      481 tcctgaagct gtacctgggc caggggtttg tcaaaggtgc ttctggggcc gaaggccagg
      541 gaatcatggt acatggactg ggctttggtg agcctggcca ttccatagga aaccagcacg
      601 ccgataatca gggggaacat catttgcccg gccatggtga attccaccac cagcaccagg
      661 gaggtgattg gggcgttcgc agctgtagtg aaaaaggcgg ccatgcccac cagggaatag
      721 gcgatggcat ggtccccggg cacccccagc tgggtaagag ccgcgctgaa caggaagccg
      781 cagacgctcc cgatagtcag gctgggggtc agggcgcccc ccacggttcc cacgccgaag
      841 acgatggcga cggtgaatat tttcaggaac agcaggacgg cggctctcgc ggcgtcgaat
      901 tcataatgaa tgagggaggt gatgatattt ttgccgttcc ctacgatttc cgggtaaaag
      961 atggcgatgg ctcccaccag cacgcctgcc gccgtcaggc gcacgggcag ccattggcgc
     1021 ttgccgttca ggtaccggcg cgattttttg aggatgagga gccagaaact ggcgaccagg
     1081 gaggccaggg cccccagcag cacgcaccag agcacatgct ggttgcctgc cagagagact
     1141 tcctgctgga gctggtagat gggggcgggt tcatggaaca ggcccatggt aaaatagccc
     1201 gcgcagctgg ccacgagcag gggagccagg aaatcaatcg tcagcgcgcc cagaacgatt
     1261 tcacttacga acaggccgcc cgccaggggc gtatggaagg cggaggacat gccggaggcg
     1321 gcggcgcagg ccactaccag gcgcaagcga ggagctgaga ggtgaaagaa acgccccagg
     1381 gcggagcccg ctacggcggc ggcctgcacc agcgggcctt cacggccgat ggaggcgccg
     1441 gaacctacgg agaaaacggc ggacagggaa cgcagaaggc tgggcttggg aggcacgtat
     1501 ccattgccca gcgccacggc ttccatgtat tccgtcgcct tggccggcac aaagcggtgg
     1561 gtgaaaagaa gcgtaagccc cgccaaaagg ccgcccgccg ccggaacgaa gatgcaccag
     1621 gccgggtcct ggccggccat ttcctgaaag acctgaattt gcctgtagct ggtccgcccc
     1681 gtcaggacgt cctgcaccag ttccacaccc atttcaaaga caagggccat cagggcgccc
     1741 agcgctccga cgacgaccgc ccagatgaag atgacctgct tgtcccccag cttgactttt
     1801 tcacgcaggt tcaacgccat gcgcagccat gaccaggaaa ggcgcgtctt ttccgcaggc
     1861 tggtttttct tgtccgtcat tgcagggctt gttgaaggga cgccaggagg ctggcttcat
     1921 ctcccgtcca ttccccgttg gggcccaggg gggcgttttt atgccaccgc agcaatttct
     1981 gcatggtggt caggtagccg gtcaccatgg cggcgggcac gcccagcatc acaatgagat
     2041 gggcgttgcg ggaatcgtcc ggccagagga cgccctctgg gctgatgcct acggtgacct
     2101 gcatggaatc cagcgcgctg gtgcgtccgt gggggacggc caggccgtga tccagatagg
     2161 tggtctgcgt ttcctcacgc agaagaaccg cattcttgat gtgtcttagc tgtcggtcgt
     2221 caaggacttc cgccatggaa tccgtcaggg tttccagagc ctgtgcctga gtggttgagg
     2281 gctggaacgt gcgtacgtga atttgaagat tgtctgccac cgggtcttct gaaggctttt
     2341 gattttccat tctatggagg gaggcatgat tatcaaggaa attctggcct gcatacgggg
     2401 gaaggaggct ttcccccgtg aatgggaggc gggaaaaagg ggg
//
LOCUS       DAOMSU010000318         2425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4524_length_2425_cov_2.466245, whole genome shotgun sequence.
ACCESSION   DAOMSU010000318 DAOMSU010000000
VERSION     DAOMSU010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4524_length_2425_cov_2.466245"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PK077_10055"
     CDS             complement(<1..367)
                     /locus_tag="PK077_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24680.1"
                     /translation="MLIGSRIITCHVTMEHTPSLPSGFNINGYLIQSLKQTDSLCHVY
                     YASDADHVPYLLREFCPQGLAVRDPESGKLRYPENTDIEREVLPLKNDFEAQFRTGSL
                     GEIPALGTLYLAYAIPGGHA"
     gene            505..1815
                     /locus_tag="PK077_10060"
     CDS             505..1815
                     /locus_tag="PK077_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRN24681.1"
                     /translation="MSFDIRLDALQYHSQPRPGKVETLPCKPCFSQRDLTLAYSPGVA
                     EPCLRIQEDPSQSALYTGRSNLVGVITNGTAVLGLGNIGPDAAKPVMEGKGVLFKVFA
                     DIDVFDIELNVKEPEKLIETIKTMEPTFGAINLEDIKAPECFMVEERLRQEMNIPVFH
                     DDQHGTAVISGAALLNAAELTGRKLEDMKVVVVGAGAAGISCAKFYMTLGVRREHIYM
                     FDSKGLIHTGRIDLHATKAQFSQSEDCSLGEALTGADVFLGLSTKGLLTQDMVKLMAP
                     SPIIFACANPDPEITYQDAKKARPDCIMGSGRSDWPNQVNNVSCFPFIFRAALDVRAS
                     VINEQMKIAAARSLADLAKEPVPQEVIDLYGGAPLSFGIDYVIPKPIDPRIIEWECPA
                     VAQAAMISGVAQSPIRDMEAYTLELRKRIAAARERVAGVVRSYL"
     gene            complement(2243..>2425)
                     /locus_tag="PK077_10065"
     CDS             complement(2243..>2425)
                     /locus_tag="PK077_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24682.1"
                     /translation="QPHAHSFKAIAEQWGMRYLAIRTAEDFDQLDSLEENSQTLVELI
                     PDREQTEQMRLRLANA"
BASE COUNT          492 a          648 c          719 g          566 t
ORIGIN      
        1 cggcatggcc gccgggaatg gcataagcca ggtacagggt gcccagagcg gggatttccc
       61 ccagggagcc cgtgcggaac tgcgcttcga aatcattttt cagcggaaga acttcccgtt
      121 caatatccgt gttttccgga tagcgcagtt ttccgctttc cgggtcgcgc acagcgagcc
      181 cctggggaca gaattcgcgg agcaggtatg gaacatggtc ggcatccgaa gcgtaataga
      241 catggcagag ggaatccgtc tgcttcaggg attggataag atagccgttg atattaaatc
      301 cggaagggag agaaggcgta tgttccatgg tcacatgaca ggtgattatc cgactgccta
      361 ttagcatatc cggaggcttc tatcaatgta aaaagaggtt ttctggggcc ggagcgtgtg
      421 acgcgattaa ttgattattt gcttgaatgc atggcgatta tgtggtgcaa gcaaggctcg
      481 cttttttgtt tttccattac aatcatgagt ttcgatatta gattagacgc cttgcagtat
      541 cattcccagc cccgccccgg caaggtggaa acgcttccct gcaagccctg cttttcacaa
      601 cgggatttga cgcttgccta ttccccgggc gtagccgaac cctgtctccg cattcaggaa
      661 gatccttccc agagcgccct gtataccggg cgttccaatc tggtgggcgt gatcaccaac
      721 ggcaccgccg ttctggggct gggcaatatc ggcccggatg cggccaagcc ggtgatggaa
      781 ggcaagggcg ttttgttcaa ggtattcgcg gacattgacg tttttgacat tgagctgaac
      841 gtgaaggagc cggaaaagct gattgaaacg atcaagacca tggagcccac cttcggcgcc
      901 atcaatctgg aggacatcaa ggctccggaa tgcttcatgg tggaggaacg cctccgtcag
      961 gagatgaata ttcccgtgtt ccatgacgac cagcatggaa cggcggtgat ctccggcgcc
     1021 gcccttttga acgcagccga gttgacgggc cgcaagctgg aggatatgaa ggtcgtcgtc
     1081 gtcggggccg gggccgccgg catttcttgc gccaagttct acatgacgct gggagtgcgc
     1141 cgcgaacata tctatatgtt tgactccaag gggctgattc ataccgggcg cattgacctg
     1201 cacgccacga aagcccagtt ctcccagtcg gaagactgtt ccctggggga ggccctcacc
     1261 ggggcggacg tgttcctggg gctttccacc aaggggttgc tcacgcagga catggtcaag
     1321 cttatggctc cttcccccat cattttcgcc tgtgcgaatc cggacccgga aatcacttac
     1381 caggatgcca aaaaagcgcg gcctgactgc atcatgggat ccggacgttc cgactggccc
     1441 aaccaggtga acaatgtttc ctgcttcccc tttattttcc gtgccgccct ggatgtgcgc
     1501 gcttctgtca tcaatgaaca gatgaagatc gccgccgccc gctccctggc tgatctggcg
     1561 aaggaacccg ttcctcagga ggtgattgac ctttacgggg gagccccgct cagcttcggc
     1621 atcgattacg tgattcccaa gcccattgat ccccgcatca ttgaatggga atgcccggcg
     1681 gtagcccagg cggccatgat ttccggcgtg gcccagtccc ccatccggga tatggaggcc
     1741 tatacgctgg aattgcgcaa gcgcattgcc gcggcacgtg aacgcgtcgc cggcgtggtg
     1801 cgcagctacc tttaacgggg atcccggcgg gaagccggaa aacccatgcc tccggcgttc
     1861 gcgccgggcg gttgcaaaaa ccgggtggac tgatgttccg cccggttttt gcatgcccgt
     1921 acagggaagt gtttgtttgg cggggatact gcatcagagc attctggagg cggaaagagg
     1981 gattgcgggc gacgcttcct tccgtatgaa ttaagacgga catgctgttg cccggcgttt
     2041 ccctgttgaa tgcggcttgg tgaataccac catagtgagg ctgttttctt ctcttcgtca
     2101 ggcaagaaag aaattcccct atggtcagat ggaggaaatg ttatttttat ctggtatttt
     2161 caagccatgt ggagagaacc gcaagatatg gccgggcgga ggatcgtctt cgctccggtc
     2221 tttggtaaag gcttgtcttc atttaggcgt tagccagccg gaggcgcatc tgttccgtct
     2281 gttccctgtc cgggataagt tctacaagcg tctggctgtt ttcctccagg gaatcaagct
     2341 ggtcgaagtc ctccgccgtg cggatcgcca ggtagcgcat tccccattgt tccgcaatgg
     2401 ccttgaagga atgggcgtgg ggttg
//
LOCUS       DAOMSU010000319         2402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4592_length_2402_cov_2.407755, whole genome shotgun sequence.
ACCESSION   DAOMSU010000319 DAOMSU010000000
VERSION     DAOMSU010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4592_length_2402_cov_2.407755"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>331)
                     /locus_tag="PK077_10070"
     CDS             complement(<1..>331)
                     /locus_tag="PK077_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRN24683.1"
                     /translation="LVAYCKARGIQVLPEVEMPGHNMALAASYPEFCCNTKRAQVWTH
                     GGVSSKLICPQKPATKKFLKDTFHTVQQIFPFPYIHIGGDECPMGDWKKCPDCQAARA
                     KKGQGDNV"
     assembly_gap    332..347
                     /estimated_length=16
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<348..922)
                     /locus_tag="PK077_10075"
     CDS             complement(<348..922)
                     /locus_tag="PK077_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRN24684.1"
                     /translation="MKSILLAAAFLGSICWAGTNPYNIIPEPVNVTTASGTTKNLKIV
                     HEQKVAGLGNEGYTMKLTPGGVELRYTTPNGKAMAMATLLQLQDQLSDTPEGLPCGSI
                     QDSPDFGWRGMMVDVGRYHYPMKEIYNFVDAMHYYKYNVLHLHLTEDQGWRLPVPGYD
                     KLRTIGAVRPSAPASQNNSLLANEGMYTKKEL"
     gene            complement(1036..>2402)
                     /locus_tag="PK077_10080"
     CDS             complement(1036..>2402)
                     /locus_tag="PK077_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRN24685.1"
                     /translation="IVGKWHLEAIPPDAKGDTSKYESNPTGFDYWEIFPGQGNYFNPD
                     FITPGKDGKRVVKTEPGYATELVTQKSLKWLDQRDKDKPFMLVVGHKAPHRCWCPSIQ
                     NLGRAKQYADAIDPPANLEDDFADRPEFLKMTEQTLLNHFNVWSDEHLIKEVVPEDIQ
                     KMLSCPESKTLHTQYDWEMPEWVRMDPQQKEAWYNYHKARTVQLVKDIKNGKIKTQRD
                     ILLRRWRHYMEDYLGTVLSVDESIGQIMDYLKRNGLDRNTLVLYCGDQGFYMGEHGLY
                     DKRWIFEESFRMPLIMRWPGHIKPGVRSSSIVQELDYAPTFCEVAGVNTKENMNTFQG
                     RSLTPLFQTGEHQNFKNRPIYYAFYENPGEHNAPRHDGLRTGRYTLSYIWTSDEWMLF
                     DNQKDPAQMHNVINKPEYAETVKELKALYGKLRKDYQVPEGFPGATGKLAVKPQWDCA
                     PSRD"
BASE COUNT          441 a          608 c          725 g          612 t
ORIGIN      
        1 cacattatcc ccctggccct ttttggctcg ggcggcctgg cagtccgggc actttttcca
       61 gtcccccatg gggcattcgt caccgccgat gtggatgtac gggaaaggga atatctgctg
      121 aacggtatgg aaggtatcct taagaaactt tttagtggcc ggtttctgcg ggcaaatcag
      181 cttggaggaa acaccgccgt gcgtccatac ctgggcccgt ttggtgttgc agcagaattc
      241 gggataggat gcggccaggg ccatgttatg gcccggcatt tccacttccg ggagcacctg
      301 gataccgcgc gctttacagt aggctaccag gnnnnnnnnn nnnnnnnagc tccttcttgg
      361 tatacatgcc ttcattggcc agcagggagt tgttctggct tgccggggcg gagggacgga
      421 cggcgccgat agtgcggagc ttatcgtagc ccggcaccgg gagccgccag ccctggtctt
      481 ccgtcagatg gagatgcagg acgttgtatt tgtagtaatg catggcgtcc acaaagttat
      541 agatctcctt catggggtag tggtagcggc ccacgtcaac catcatgccg cgccagccga
      601 agtcggggga atcctggatg ctgccgcagg gaaggccttc aggagtatct gaaagctggt
      661 cctgaagctg gagcagggtc gccatcgcca tggccttccc gttgggcgtg gtataacgga
      721 gttccacgcc gccgggcgtc agtttcatgg tatacccttc attgcccagt ccggcgactt
      781 tttgctcgtg gacgattttg aggtttttgg tagttccgga agccgtcgtc acgttgacgg
      841 gctccggaat aatgttgtag ggattggttc cggcccagca gatggagccc aggaacgcag
      901 cggctagcaa aattgatttc atgcggcgcg tgtggtaatg gttgttgttt gtgtgaggag
      961 ttaaaccttg tctttccgcc gtcaatggcg tgttccggat aacccgtgtt tcgggaggaa
     1021 cggtgaatgg cggcgtcaat ctctggaggg agcgcagtcc cactgcggct tgacggccag
     1081 tttgccggtg gctccgggga agccttccgg cacctggtag tctttgcgga gcttgccgta
     1141 cagggcttta agctctttca cggtttccgc atattccggt ttgttgatga cgttgtgcat
     1201 ttgggccgga tccttctggt tgtcaaagag catccattcg tcgctggtcc agatatagga
     1261 gagcgtataa cggcccgtgc gcagtccgtc atggcgcgga gcgttgtgtt cgcccggatt
     1321 ttcgtaaaag gcgtaataga taggacgatt cttgaaattc tgatgttccc cggtttggaa
     1381 caggggagta aggctgcggc cctggaaggt gttcatattt tccttggtat ttaccccggc
     1441 tacctcgcag aaagtggggg cataatccag ttcctgcact atggaggagg agcgcacgcc
     1501 tggcttgatg tggcccggcc atctcatgat gaggggcatg cggaaggatt cttcaaaaat
     1561 ccagcgcttg tcgtacaggc cgtgttcccc catgtagaat ccctggtctc cgcagtagag
     1621 aaccagcgta ttcctgtcca ggccgttccg tttcagataa tccatgatct ggccgatgct
     1681 ttcatccacg gaaagaacgg tgcccagata gtcttccata taatggcgcc agcggcgcag
     1741 caaaatgtcg cgctgcgttt tgattttccc gtttttaata tctttgacaa gctgtacagt
     1801 acgggccttg tgatagttgt accaggcttc cttctgctgc gggtccatgc gcacccattc
     1861 cggcatttcc cagtcatact gggtatgcag ggtcttggat tccgggcagg aaagcatttt
     1921 ctggatgtct tccgggacca cctccttgat caggtgttcg tcagaccata cgttgaaatg
     1981 gttgagcagg gtctgttccg tcatcttcag gaattccgga cggtctgcaa aatcgtcttc
     2041 cagattggcg ggcgggtcaa tcgcgtccgc atactgtttg gcgcggccca gattctgaat
     2101 ggaggggcac cagcagcggt ggggagcctt gtggcccacg acgagcatga aaggcttgtc
     2161 cttgtccctc tggtccagcc atttgaggct tttttgcgtg accagttccg tggcatagcc
     2221 gggttccgtt ttcaccacgc gcttgccgtc tttgccggga gtgataaaat ccggattgaa
     2281 atagttgccc tggccgggga aaatttccca gtaatcgaat ccggtggggt tggattcata
     2341 tttggacgtg tctcccttgg cgtccggcgg aatggcttcc agatgccact tgccgacgat
     2401 gg
//
LOCUS       DAOMSU010000320         2402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4596_length_2402_cov_1.809544, whole genome shotgun sequence.
ACCESSION   DAOMSU010000320 DAOMSU010000000
VERSION     DAOMSU010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4596_length_2402_cov_1.809544"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..212
                     /locus_tag="PK077_10085"
     CDS             63..212
                     /locus_tag="PK077_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24686.1"
                     /translation="MVFCGGVIPVRDYDFLKENGVVVIIFGPGTNIPEASREIMEALN
                     EQYAD"
     gene            576..1274
                     /locus_tag="PK077_10090"
     CDS             576..1274
                     /locus_tag="PK077_10090"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN24687.1"
                     /translation="MKKLFVSSIMTAVLLPGWVNAESPNLVTLLSLTKPQNGVLSSGN
                     SSLTWSEGTTGTSLDNFAISFQLTETRNTSSPQNIFSTSRTAGGASGNALQLTSSSAD
                     GGYTCLFSYAGQSFDMTATTERGSLLLTLIMRKSQDGSSALATLYSGTQTLRTVSFNF
                     TSGTSSLKWDQSSIWTNAAKETFTDIKLATFNTEAADTDILNAFGLQVPEPATASLSL
                     LGLAALMVRRRRKA"
     gene            1476..2363
                     /locus_tag="PK077_10095"
     CDS             1476..2363
                     /locus_tag="PK077_10095"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRN24688.1"
                     /translation="MERNYHYCVERNRELIPPDTDMFRVMDGAEDGIPGVFVDQMADR
                     ILVSTRGCSITADLESELRDTGLPVFHKKLEQNQKEAPVQIAGPLLPLQFTALEQGVR
                     FKIDMSAGYSQGIFLDQRDHRRRVREKSAPGMRVLNTFAYTGAFSVYAALGGAQTTTL
                     DLAQPCLDWARENFVLNGIDPSGQYFCKGDARRWLERFSRQGRTFHGIILDPPTFSRD
                     DRGKVFRVESHYGELVALARECLEPGGWMLCTSNCRKLSHEEFRRMVARAVPGFRLTR
                     DPMPPDFSGEDYLKRLWID"
BASE COUNT          558 a          608 c          626 g          610 t
ORIGIN      
        1 cgggcctgcc cgtattccac aaaaaactgg aacaaaacca gaaagaaacg ttctgaacat
       61 ccttggtctt ctgtggcggc gttattcccg tccgggacta tgacttcctg aaggaaaacg
      121 gagttgtggt catcatcttc ggcccgggca ccaacattcc ggaagcttcc agggaaatca
      181 tggaagctct taacgaacag tatgctgact aaacggaatt tcctgtttat caggtaaata
      241 cctgttgcga gaggccggat gaaaaatccg gcctcttttt tgcgggcgga ttgactatcc
      301 gctgaagcag ttcagcactg ctgtaaaaat tcagaaacaa taatgagata attaagttgt
      361 taagtgtcaa taactttcaa acaacataaa attcctttct tctcaatgaa atcacaaaca
      421 tttcttgaat tcatgaatga gttgcggagc aagatgcttg cggacatttt ttgttcttct
      481 gtatttttta tcaggctttc cctccctatt atctgcataa attcttttag aaaaagttga
      541 cagcggatag tcaatccggc tataagctgt ccgtgatgaa gaaattattt gtatcgtcta
      601 ttatgacggc ggttttgctg cccgggtggg tcaacgcaga aagtcctaat cttgttacgc
      661 ttctgagcct tactaaacct cagaacggtg ttctttcctc tggcaattcg tcattgacgt
      721 ggagtgaagg aacaacagga acgtccttgg acaatttcgc catttctttt cagcttacgg
      781 aaacaagaaa tacttcctct ccccaaaata ttttctcgac ctcacgtacc gccggggggg
      841 cgtctggtaa tgcactacag ctgacttcct cctccgcaga cggcgggtat acctgtttat
      901 tctcctatgc aggtcagagt tttgatatga cggcgacaac tgaaagaggc agtcttcttc
      961 tcacgctgat tatgcggaaa agccaggatg gaagctctgc cttagctacc ctgtacagcg
     1021 gaactcaaac cttgagaaca gtttctttta atttcacctc cggtacaagc tctctgaaat
     1081 gggatcaatc cagtatctgg acgaacgcgg cgaaggagac attcactgac attaagctgg
     1141 cgacgtttaa taccgaagcc gcggatacag acattctcaa cgcttttggc ttgcaggttc
     1201 cggaaccggc tacggcatcc ctgagtctgc tggggctggc ggccctgatg gttcgccgcc
     1261 gccggaaagc ctgaaaacct tgtcctgcca gcagttaaag gcgtttttgc ctgcaaggtc
     1321 cagccttgca ggcgattttt ttaatgtctc tgttgatgaa ggagggccgt ccagatgacc
     1381 gtttttcctg gcggaactct tgctccgaag gaaatgaggc tcctgcttct ttgacattgc
     1441 gggaacgttc ttcatccgtt actctttgct ccgccgtgga gagaaattat cattattgcg
     1501 tggaacgcaa cagggaattg attccgccgg atacggatat gttccgggtc atggacgggg
     1561 cggaggatgg aattcccggt gtctttgtgg atcagatggc ggatcgtatt ctcgtctcca
     1621 cgcgcggatg ttccattact gcggacctgg aaagcgaact ccgggatacg ggcctgcccg
     1681 tattccacaa aaaactggaa caaaaccaga aagaagcgcc cgtccagatc gccgggccgc
     1741 tccttcccct gcaatttacg gccttggagc agggagtgcg tttcaaaata gacatgtccg
     1801 ccggttattc ccagggaatt tttctggacc agcgcgacca ccgccgccgg gtgagggaaa
     1861 aaagcgctcc gggcatgagg gtgctgaata cctttgccta tacgggggcc ttttccgttt
     1921 acgccgccct ggggggagcg cagacaacca cgctggatct ggcgcagccg tgcctggact
     1981 gggccaggga aaatttcgtt ctgaacggca ttgacccctc cggccagtac ttttgcaagg
     2041 gggacgcgcg ccgctggctg gaacggttct ccaggcaggg gcgaaccttt cacggcatta
     2101 ttctggaccc tcccacgttt tcccgggatg accgcggaaa ggtgttccgg gtggaatccc
     2161 actacgggga gctggtggcc ctggcgcgcg aatgcctgga accgggcggc tggatgctgt
     2221 gcaccagcaa ctgccgcaag ctcagccacg aggaattccg caggatggtg gcccgtgccg
     2281 ttcccggttt ccgcctgacc cgtgacccca tgcctccgga tttctccggg gaggattatt
     2341 tgaaaagatt gtggatagat tgaaaatgaa tattttatga aaaatttccc tctctgtggc
     2401 tg
//
LOCUS       DAOMSU010000321         2400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4605_length_2400_cov_2.127079, whole genome shotgun sequence.
ACCESSION   DAOMSU010000321 DAOMSU010000000
VERSION     DAOMSU010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4605_length_2400_cov_2.127079"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /locus_tag="PK077_10100"
     CDS             <1..589
                     /locus_tag="PK077_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24689.1"
                     /translation="QQPPLASRDYAQAARFLSMAVQYRDQEALYLLSLMYSQGWGVPA
                     SPELSSLMMERLNGSSYSTDSLNPNAPIAAYWLPLPLTGEKNTILRFTIPGPFAPSQL
                     KGVTLHNTSLKDAFSISKINILQHGRLILDIISPQEVLPGKSSAPIGISIPEGLLADT
                     TLPLEVEILLTPGNSCGVVEMPPPVEKKNSGIEIN"
     gene            702..1253
                     /locus_tag="PK077_10105"
     CDS             702..1253
                     /locus_tag="PK077_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24690.1"
                     /translation="MLPMLAAFLMASCGDPTVSYWKKAADINTEYSEKSDALVQRLLK
                     LKKNPTLPGLEESSRDAADLLRERDEELADLSTKNVDPAVTAYVAEDRKLFARGRELA
                     ERYQQYFEKYLKGGPDFTPDPSRAVAHIGRGRQEIRKILAEARKLEERAEMLRKEKSA
                     ELEQELPPLHFRLPELKQLLSSR"
     gene            complement(1568..2260)
                     /locus_tag="PK077_10110"
     CDS             complement(1568..2260)
                     /locus_tag="PK077_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24691.1"
                     /translation="MALKYENNAYDPSSGNAVAFNVTMYKEPIQEGKYEGNSTMKREF
                     SPSPKSSRCNEKIFQILGFLASLFFHAPAWGDMADNGIECSVVMEKASFDPRAAFPDF
                     KLVFANKGEKAVRLFDDFYPLKERGPNMIIKIWSKGNGKKGKRPTAWYLPSYQMERVA
                     NLMRFITLNPGEKYEVPVKDAYLLLTCLQPLASGKIYELEVRFRDEYGDLGIRREYVG
                     KKDFIVIDQTPR"
BASE COUNT          608 a          694 c          539 g          559 t
ORIGIN      
        1 ccagcagcct ccccttgcct cccgggatta cgcccaggca gcgcgtttcc tgagtatggc
       61 ggtccaatac cgggaccagg aggccctgta cctgctttcc ctcatgtact cccagggctg
      121 gggcgtaccc gcctccccgg aactttccag cctgatgatg gaacgcctca atggatcttc
      181 ctactccacg gacagcctga atcccaacgc gcctatcgcg gcgtactggc ttccccttcc
      241 tcttaccgga gaaaaaaaca ccattctgcg cttcactatt ccagggccgt tcgccccgtc
      301 ccagctcaag ggcgttactc tgcacaacac aagcctgaag gatgccttct ccatcagtaa
      361 aatcaatatt ctccaacatg ggcgcctcat tctggatatc atttcccccc aggaagtgct
      421 gcccggcaaa agctccgctc ccataggcat atccatcccg gaagggctgc tggcggatac
      481 gaccctgcct ctggaagtag aaattttgct gacccccgga aacagctgcg gcgtggtgga
      541 aatgccccct ccggtggaaa agaaaaactc cggaattgaa atcaattaag cttcaccgct
      601 tttcaacgcg aaacagcaac agggaaaacg gaaaacaccc ttgattcccg cctggggcat
      661 gctacaaaag ataaatgaat gcctctgttt tcctgcggcg tatgctccct atgctggccg
      721 cgttcctgat ggcctcctgc ggtgatccta ccgtctccta ctggaaaaag gcggcggaca
      781 tcaatacgga atactcggaa aaaagcgacg cactggtgca acgcctcctg aaactgaaaa
      841 agaaccctac ccttccgggc ctggaagaaa gcagcaggga tgcggctgac ctgttgcggg
      901 aaagggacga agaactggcg gacctgagca cgaaaaacgt ggatcccgcc gtgacggcct
      961 atgtggcaga agaccgcaag ctttttgcca gaggaaggga attggcggaa cgctaccagc
     1021 aatactttga aaaatatcta aagggaggac cggattttac tccggatcct tcccgggccg
     1081 tcgcccatat aggccgaggc cggcaggaaa tacgcaaaat tctggcagaa gcccgaaaac
     1141 tggaagaacg ggctgaaatg ctgagaaaag aaaaatcagc tgagcttgag caagaacttc
     1201 ctccgctgca ctttcgcctt ccggagttga aacaacttct atccagccgt tagaaaacgc
     1261 gcacgaacca cttgcatcaa tgggagcctc ggccagtctg acagcggcct ttacactgca
     1321 cgggcggaca caaccggctc ctgaaacttt ccccgtccat gttacggaaa tatcccttcc
     1381 ctggatttac aggaaaaacg agtccccctg ctgtccatcg tcgtttattt cagcattcaa
     1441 atttcattcc ctgaataccc cttagcacag gcttcatagt ttaaaattcc aaagaaaggg
     1501 tttgcctccc ctccacaaaa aagccccgtt catatttcat gaacggggct atattaattt
     1561 ttcaaattca cctgggagtt tgatcaatga cgataaaatc ctttttcccc acatactccc
     1621 tccggatacc cagatctcca tattcgtccc ggaaacgtac ttccaattcg tatattttcc
     1681 cgctggccag aggttgtaaa caggtcagca gcaggtatgc atccttgacg ggcacttcat
     1741 atttttctcc ggggttcagg gtaataaagc gcataagatt cgcgacacgc tccatttgat
     1801 aggagggaag ataccatgcc gttggccttt ttcccttctt tccgtttccc ttactccata
     1861 tcttgatgat catattcggc ccgcgttcct tgagaggata gaagtcgtcg aaaagacgca
     1921 cagccttttc tcccttattg gcaaacacca gcttgaagtc cggaaaagcg gctcttggat
     1981 cgaacgaagc cttttccatc accacggaac attcaatccc gttatcggcc atatcccccc
     2041 atgcaggagc atggaagaac aggctggcca aaaaccctaa aatttggaag attttttcat
     2101 tgcagcgcga gcttttggga gaaggcgaga attcgcgctt catcgtcgag ttcccttcgt
     2161 acttcccttc ctggataggt tccttgtaca tcgtgacgtt gaacgcgacc gcattgccac
     2221 tagagggatc gtaagcgtta ttctcatact ttaaagccac cttgtaataa aatgtctcac
     2281 tatctccagg gcgattgatc gaccacggag aaatcttttc ttcctacata cttcctccga
     2341 acaccgggat ctccatatcc gtcacgaaag attacttcca actcatattt ctcaccattg
//
LOCUS       DAOMSU010000322         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4606_length_2399_cov_1.768345, whole genome shotgun sequence.
ACCESSION   DAOMSU010000322 DAOMSU010000000
VERSION     DAOMSU010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4606_length_2399_cov_1.768345"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="PK077_10115"
     CDS             <1..514
                     /locus_tag="PK077_10115"
                     /inference="COORDINATES: protein motif:HMM:NF024352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TaqI-like C-terminal specificity
                     domain-containing protein"
                     /protein_id="HRN24692.1"
                     /translation="LMEARRETRMGRMKYYHVHWPRQERFFRPGPKILAVRKCARPTF
                     SYTEKEAYVMMAFNVIRSERVSMKYLAALFNSRLMQFWFLRRGKMQGDFFQMDTAPVL
                     RAPIRVPGLSLLREAERLADALAVRYCPEEDERMNELMEDIYGLSPQEREVVIQACSS
                     MRAGRESLPE"
     gene            772..>1213
                     /locus_tag="PK077_10120"
     CDS             772..>1213
                     /locus_tag="PK077_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24693.1"
                     /translation="MMKLVFPGMSLLAVCWCAAFSSAEAGVEAAQSQPVPGESLPSLC
                     RVLDRGPVLYRNDSSWIRKVKLTLIGQYQAAAVSPNGANKFCASSGGHNNGWRRAYLG
                     GDILMGDGSWRLSNLTNVGDLEGRHREVRGEWIGSHTEWSLYELY"
     assembly_gap    1214..1245
                     /estimated_length=32
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1246..2068
                     /locus_tag="PK077_10125"
     CDS             <1246..2068
                     /locus_tag="PK077_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24694.1"
                     /translation="EKTMPGVKFRAGKLTPHLTSEYCLASSRIKTVERSALCNELIPI
                     SNWGLEANFQKDAKSLYHSYGIYLNANGTDLKDEIQFHSADNLFILNAMKWKVASPMW
                     DSQFLGYQYAHNFTEWRGRKIPSASDYCGTGAQDVISLSWDASRGAFSIMGNLLAGVG
                     IVGQPGAKNVYGLVLQPVYRISPHLEGVFQYQCSFGNRSVKLNTRYVPSVTHYPAWVD
                     SMHSFYLGLNCYLCPEAVNAVKLMLAVEYVTSHVDSATAKAFNGWSVFGAVRFKF"
     gene            complement(2085..>2399)
                     /locus_tag="PK077_10130"
     CDS             complement(2085..>2399)
                     /locus_tag="PK077_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24695.1"
                     /translation="PFFHTPRSAANTEIREIPMPSDSWPAWNEEEVNLHMNLPESAPR
                     PLVVQPAPSGAIRTIIKSFNGTVFEADAQPMHPVWDQLPPGYVPIPALPAYQPTLLKK
                     LQ"
BASE COUNT          478 a          590 c          731 g          568 t
ORIGIN      
        1 cctgatggag gcccggcggg aaacacgcat gggccgcatg aaatattacc atgtgcactg
       61 gccccggcag gaacgttttt tccgtcccgg ccccaaaatc ctggctgtca ggaagtgtgc
      121 acgtccaacc ttttcctata cggaaaagga ggcttatgtg atgatggcat tcaacgtgat
      181 tcgctcggaa cgcgtgagca tgaaatatct ggcggccctg ttcaattcac gcctgatgca
      241 gttttggttt ctccgccggg gaaaaatgca gggggatttt ttccagatgg ataccgcccc
      301 tgtcctccgg gctcccatcc gggtccccgg tctgtccctg ttgcgggagg cggaaaggct
      361 ggcggacgcg ctcgcggttc gctattgtcc ggaagaggat gagcggatga atgagctgat
      421 ggaagacatt tacggtcttt ccccgcagga gcgggaggta gttattcagg catgttcctc
      481 catgcgggcc gggagggaaa gcttgccgga atgatgcgtt ggagccggta aacgttagcg
      541 atgatgccag ggaacttcat tatggtttct ccatcgcgtc gggaatgatg aggaataacc
      601 ccattcgtca ggccgatgat atttcccgga ggggcagggc gtaccgctgc atccaccatt
      661 gccgttcttt gtaacaaccc tttcagaaac ctttgatgtc gaaaggaaca tccgggcttg
      721 attttcgaag gcggctcctt aagatgtgcg cccgatttgt ttgtttagcc tatgatgaag
      781 cttgtttttc cggggatgtc cctgttggcc gtttgctggt gtgccgcctt ttcttcggcg
      841 gaagccggtg tggaagcagc tcagtctcag ccggttcccg gggaatctct gccgtccctg
      901 tgccgtgtgc tggatcgcgg tccggtgctg tacaggaatg attcctcatg gatcaggaag
      961 gtgaagctga cgctgattgg acagtatcag gcagcagccg tcagccccaa tggagccaat
     1021 aaattctgcg cttcttccgg cggccataac aacgggtgga ggcgcgctta cctgggcggc
     1081 gacatcctga tgggggacgg ttcctggcgc ctgtccaatc tgacgaatgt gggggatttg
     1141 gaaggccggc accgtgaagt gcggggagaa tggatcggca gccatacgga gtggtccctg
     1201 tatgaacttt atcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggaaa agaccatgcc
     1261 cggcgtcaag ttcagggccg ggaagctgac gcctcacctg acgtcggagt attgcctggc
     1321 ttcctcccgg attaaaacag tggaaagatc cgccctgtgc aatgaattga ttcccatttc
     1381 caactggggt ctggaggcca attttcagaa ggatgccaag agcctgtacc attcctacgg
     1441 catttacctg aatgcgaacg gtacggattt gaaggatgaa atccagtttc attctgcaga
     1501 caacctgttt atcctgaacg ccatgaagtg gaaggtggct tctcccatgt gggacagcca
     1561 gtttctgggt taccagtatg cccataattt tacggaatgg cggggaagga aaatcccctc
     1621 cgcgtccgac tactgcggaa cgggggcgca ggacgtgatt tccctgagct gggacgccag
     1681 ccgcggcgct ttctccatca tggggaattt gctggcaggc gtgggaattg taggccagcc
     1741 cggggcaaag aatgtgtacg gactggtgtt gcagcccgtt taccggattt ccccgcatct
     1801 tgagggcgtc tttcaatacc agtgttcctt tggaaaccgt tctgtgaagc tgaatacccg
     1861 ttatgtgccc agcgtcaccc attatcccgc atgggtggac agcatgcatt ccttttacct
     1921 ggggctgaat tgctatctgt gtccggaggc tgtcaatgcg gtcaagctga tgctggctgt
     1981 agagtatgtc accagccatg tggattcggc aacggccaaa gctttcaatg gatggtctgt
     2041 tttcggggct gtccgcttca agttctgagg ctttctgccc acttctattg taattttttc
     2101 aataacgtgg gctgataggc cggaagagcc gggattggga cgtacccggg cgggagctgg
     2161 tcccagacgg gatgcatggg ctgggcatcc gcctcaaaaa cggttccgtt aaaggatttg
     2221 atgatggtgc ggatggctcc ggacggagcc ggctgcacta ccagggggcg cggcgccgat
     2281 tccggaagat tcatgtgcag gttgacttcc tcttcattcc aggcgggcca ggaatcggaa
     2341 ggcatgggga tttcacgaat ttccgtattg gcggcggaac ggggcgtatg gaaaaaggg
//
LOCUS       DAOMSU010000323         2387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4637_length_2387_cov_2.876930, whole genome shotgun sequence.
ACCESSION   DAOMSU010000323 DAOMSU010000000
VERSION     DAOMSU010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4637_length_2387_cov_2.876930"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1188
                     /gene="atpA"
                     /locus_tag="PK077_10135"
     CDS             <1..1188
                     /gene="atpA"
                     /locus_tag="PK077_10135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HRN24696.1"
                     /translation="PIEAEAYYPVEKIASGIISRQSVSVPVQTGILPIDAMIPIGRGQ
                     RELIIGDRATGKTAIAMDTMIAQAEQNRLAEEGKLPDHQPLYNIYVAIGQKRANISRL
                     VAKLEETGAMKYSIVVAASASDPAAMLYLAPYAGCAMGEYFMDKGEDALIVYDDLSKH
                     AVAYRQISLILRRPSGREAYPGDVFYLHSRLLERAARINKEHGGGSLTALPIIETQAG
                     DVSAYIPTNVISITDGQIFLETDLFYQGVRPAINVGISVSRVGSSAQTKIIKKLAGSI
                     KLDLAQFTELQAFAQFGSDLDPSTKAKLARGQRIVELFKQNQYEPKSLGLEAADLYAM
                     QKGYFDDVPVDRIKECQNAWEAVMQDQHPDLLESILKEKDLTPEIDAGLKAAIESFKL
                     SWN"
     gene            1210..2082
                     /gene="atpG"
                     /locus_tag="PK077_10140"
     CDS             1210..2082
                     /gene="atpG"
                     /locus_tag="PK077_10140"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HRN24697.1"
                     /translation="MGNLRDIRRRIKSVKNTSQITRAMQMVASAKMRRAQDQAVKGRP
                     YIRALAEVLYHLQDEIDTSNSPLMQTHGGADLVLLVNTDRGLCGGLNANLIKMVREQA
                     PENAHYITIGRKLNAALAKLNERLEATWSLTDPLSLLELKPVFDFIVQKFKAEEYGRV
                     FVAFSGFVNTMVQKPVFRQLLPIEPEPLMNMAKAGGSSLDGAEAHKQFLLEPSPAALL
                     DTILPLYVFHGLVQIVLEARASEHSARMVAMKGATENAKNIIGGLTLDYNKARQTQIT
                     NELLEITTAMRAME"
     gene            2157..>2387
                     /locus_tag="PK077_10145"
     CDS             2157..>2387
                     /locus_tag="PK077_10145"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419559.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. The beta chain is a
                     regulatory subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HRN24698.1"
                     /translation="MMNNTGILVQIIGAVVDADFSQAETLPALLNALEVDYEVDGRRK
                     TLVLEVQQHIGDGWVRAVAMSSTDGLRRGMPVR"
BASE COUNT          535 a          725 c          659 g          468 t
ORIGIN      
        1 ccgattgagg cggaagcgta ttacccggtg gaaaaaatcg cctccggcat catctcccgc
       61 cagtccgttt ccgtgcccgt ccagacgggc atccttccca ttgacgccat gattcccatc
      121 ggccgcggcc agcgcgaact catcatcggg gaccgtgcca ccgggaagac cgccattgcc
      181 atggatacca tgattgccca ggcggaacag aaccgtctgg cggaggaagg caagcttccg
      241 gatcaccagc ccctctacaa tatttacgtg gctatcggcc agaagcgcgc caacatcagc
      301 cgcctggtgg cgaaactgga ggaaacggga gccatgaaat attccatcgt cgtggcggct
      361 tccgcctccg atcccgccgc catgctgtac ctggcgccct acgccggctg cgcgatgggg
      421 gaatacttta tggacaaggg ggaagacgcc cttatcgttt atgacgacct ttccaagcat
      481 gccgtggcct accgccagat ttccctgatt ctgcgccgtc cctccggccg cgaggcgtac
      541 ccgggggacg tattctacct gcacagccgc ctgctggaac gcgccgcgcg catcaacaag
      601 gaacacggcg gcggttccct gacggcgctt cccattattg aaacgcaggc aggcgacgtt
      661 tccgcctaca ttcccacgaa cgtcatttcc atcacggacg gccagatatt cctggaaacg
      721 gacctgtttt accagggggt gcgcccagcc atcaacgtgg gcatttccgt ctcccgcgtg
      781 ggttcttccg cccagacgaa aattatcaag aagctggccg gctccattaa gctggatttg
      841 gcccaattta cggaattgca ggcgttcgcc cagttcgggt ctgatctgga ccccagcacg
      901 aaggccaagc tggctcgcgg ccagcgcatc gtggagctct ttaagcagaa ccagtatgag
      961 cccaaatccc tggggctgga ggccgctgac ttgtatgcca tgcaaaaggg gtactttgac
     1021 gatgttccgg tggacaggat caaggaatgc cagaacgcgt gggaagcggt gatgcaggat
     1081 cagcatccgg atctgctgga aagcatcctg aaggaaaaag acctgacgcc ggaaatagac
     1141 gcagggctta aagccgccat cgaatccttc aagctcagct ggaactaacg attacgggag
     1201 tactgacaca tgggcaatct gagagatatc agacgccgga tcaagtccgt caaaaacacg
     1261 tcccagatca ccagggcgat gcagatggtg gcttccgcca aaatgcgccg cgcccaggac
     1321 caggccgtca agggccgccc ctacatccgc gccctagcgg aagtgctcta ccatctccag
     1381 gatgaaattg acaccagcaa cagccccctg atgcaaaccc acggcggcgc ggatctggtg
     1441 ctgctggtga atacggaccg cggcctctgc ggcggcctga acgccaacct gatcaaaatg
     1501 gtccgggaac aggctccgga aaacgcccat tatatcacca tcggacgcaa gctgaatgcg
     1561 gcgctcgcca aattgaatga acggttggag gccacctgga gcctgacgga tcccctttct
     1621 ctgctggaac taaagccggt atttgatttc atcgtgcaaa agttcaaggc ggaggaatat
     1681 gggcgcgtct tcgtggcttt ctccggtttt gtgaacacaa tggttcagaa gcctgtcttt
     1741 cgccagcttc ttcccattga accggaaccc ctgatgaaca tggcgaaggc cgggggctct
     1801 tccctggacg gagcggaagc ccataagcaa ttcctgctgg aacccagccc cgccgccctg
     1861 ttggatacca tcctgcctct ttatgttttc cacggactgg ttcagattgt gctggaggcc
     1921 cgggcatccg aacattccgc ccgcatggtc gccatgaagg gggctacgga aaacgccaaa
     1981 aacattatcg gcggcctcac cctggactac aacaaggcgc gtcagacgca aattaccaac
     2041 gaacttctgg aaattaccac ggccatgcgc gctatggaat aacatggctc cgctttcttt
     2101 acccgccttt caaacagaac attctttctt ctccatttca acaccaagtt tttcccatga
     2161 tgaataacac aggcattctc gttcagatca tcggcgctgt ggtcgatgcg gacttctccc
     2221 aggcagaaac gcttcccgcc ctgctcaatg cgctggaagt ggactatgaa gttgacggca
     2281 ggcgcaagac cctagttctg gaagtccagc agcacatcgg agacggctgg gtccgcgccg
     2341 tggccatgag ttctacggac ggtctgcggc gggggatgcc agtgcgc
//
LOCUS       DAOMSU010000324         2387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4638_length_2387_cov_1.882933, whole genome shotgun sequence.
ACCESSION   DAOMSU010000324 DAOMSU010000000
VERSION     DAOMSU010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4638_length_2387_cov_1.882933"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1003)
                     /locus_tag="PK077_10150"
     CDS             complement(<1..1003)
                     /locus_tag="PK077_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRN24699.1"
                     /translation="MSEAPQKKGTSWSIGIGLGIVALLIGTAIVTEPSVSFSFSGNGG
                     CAPAGTETPLRLQTNTLSISAPGIVKASYLTMLSPSVSGKVDKVHPLFNVGEIVLKGT
                     PLMELEKFEYRARLANAQAELEQARLDVSTEQAEALKAIKKTYSSISKSGKESELVLR
                     VPQRRAVNARLNAAEAYVAEAEQALRDTVLEAPYTCQVVECSVGAGARVVAGQPVGKV
                     IPLQERMIRIPVPLEEFSALPRDEQGKVNTALTASCVLNNGKRLQWLGRVTAVDAALD
                     SKSNSAVLIASLEPNASHVSEWQVAPVNMALQVAINVQVPVSAWIPASAVRDGSSIQV
                     "
     gene            complement(1031..1486)
                     /locus_tag="PK077_10155"
     CDS             complement(1031..1486)
                     /locus_tag="PK077_10155"
                     /inference="COORDINATES: protein motif:HMM:NF022182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HRN24700.1"
                     /translation="MENQHLSLLDRVYTVLRKRGEETEWITSADDHNIKTGIAFVPCD
                     TFTPVSLLYTMIERPETLVIDVLFAAKVPENRRVEVSIILSELNADQTAGAFQLDPNS
                     GYVYYRQSFVLEGMDLTEAQFAQLMKNIETVAVETAEAYSHIMETEYPK"
     gene            complement(1717..>2387)
                     /locus_tag="PK077_10160"
     CDS             complement(1717..>2387)
                     /locus_tag="PK077_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018555222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HRN24701.1"
                     /translation="SFAKTLRLMAGHELATEGFAKGQTGSSAMPHKMNARSCERVNGF
                     HVILKGFLMMISGLAGDQWNEGDVSCSVVRRVVMPDSFYAADGLFETFLTVLNQMGVY
                     EAVVAAELRRYLPFLMTTTILMEAVKRGIGRETAHEVIKEHAVAVSNDLRAGKIMENN
                     LLDRLAEDGRLGIDRADLQRIFDSNSSATGMADAQVEAFRSMVKEVTDRFPDGAGYQP
                     GDIL"
BASE COUNT          508 a          699 c          665 g          515 t
ORIGIN      
        1 ttacctggat ggagctgccg tccctgacgg cggaagcggg aatccatgcg gaaacgggaa
       61 cttgaacatt gatggctact tgaagagcca tgttgacggg agccacttgc cactcggaaa
      121 catgggaggc attcggctcc agggaggcaa tcaacacggc ggaattgctt ttggaatcca
      181 gcgccgcatc caccgccgta acgcggccca gccactgcag ccgttttcca ttattcagca
      241 cgcaagaggc ggtcagagcc gtattcacct tgccctgctc atccctgggc aatgcggaaa
      301 attcttccag cggaacggga atccgtatca tccgctcctg aagaggaatt actttcccta
      361 ccggctgccc ggcgaccacg cgggcaccgg ctcccacaga gcattccacc acctggcagg
      421 tatagggagc ttccagcacc gtatcccgga gggcctgttc tgcttccgcc acatacgcct
      481 ccgcggcgtt cagcctggcg tttacggctc tgcgctgcgg cacccggagc accagttcgg
      541 attcctttcc gcttttggag atggagctgt atgtcttttt gatggctttc agggcttccg
      601 cctgctccgt agaaacatcc aggcgggcct gttccagttc cgcctgagcg ttcgccagcc
      661 gggcgcggta ttcaaatttt tccaattcca tcaggggggt tcccttcagg acaatttccc
      721 ccacgttaaa caggggatgc actttgtcca cctttccgga aacgctggga gaaagcatcg
      781 tcaggtagga ggcctttacg atgccgggcg cggaaatgga aagagtgttc gtctgcagcc
      841 ggagaggtgt ctccgttcct gcgggcgcgc agcccccgtt cccggaaaag gaaaaagaga
      901 cggacggctc cgttacaata gccgtgccta tcagaagcgc cacaatcccc aggccgatgc
      961 ctatggacca ggacgttccc tttttttgag gggcctcgct catcaacagc tccgggggaa
     1021 acgcgtcccg ttatttggga tattccgtct ccatgatgtg ggaataggcc tccgccgttt
     1081 ccacagccac ggtttcaata ttcttcatca actgggcaaa ctgggcttcc gtgaggtcca
     1141 tgccttccag aacgaaggac tggcggtaat aaacataccc gctgttgggg tccagctgaa
     1201 aggcgccggc cgtctggtcc gcattcagtt cgctgaggat aatgctgact tccacacggc
     1261 ggttttcagg aaccttggcg gcaaaaagca cgtcaatgac cagcgtctcc ggacgttcaa
     1321 tcatggtgta caggaggctc actggggtga acgtatcaca ggggacgaat gcgatgcctg
     1381 tcttaatgtt gtggtcatca gcggaagtaa tccattccgt ttcttcccct ctctttctga
     1441 gaacggtgta cacacggtcc aaaaggctga gatgctggtt ttccatattg aaaattgttt
     1501 aacagggggg ttgcttgcaa aggcgcatgc aacgtacgcg cccgtttact gaattcaagc
     1561 ttactggcat ccttctgatt cgtcaagctc tcctgacgta aagaaattga aaaaacacgt
     1621 atgcggcggc ataaaaaata cagacgccgt ccgaaaaggg cggcgtctgg aaaaaacgcg
     1681 tgcaccggga gttccggctt ctttccgagg ttctttttac aggatatccc cgggctggta
     1741 gcccgccccg tccggaaacc tgtccgtcac ttccttcacc atggagcgga atgcttccac
     1801 ctgggcgtca gccatgcccg tagcggagga attggaatca aaaatgcgct gaaggtccgc
     1861 ccggtctatt cccaggcgcc cgtcttccgc caggcggtcc agcaaattgt tttccatgat
     1921 tttcccggcg cgcaggtcat tggagacggc cacggcatgt tccttgatca cctcatgggc
     1981 ggtctcccgg ccaatgccgc gcttgacggc ctccatcagg atggtggttg tcatcaggaa
     2041 aggcaggtag cggcgcaatt ccgcagccac cacggcctca tacacgccca tctggttcaa
     2101 cacggtcagg aaagtttcaa acagtccgtc cgcagcgtaa aaggaatcag gcatgacgac
     2161 acggcgcacc acggagcagg aaacgtcccc ttcattccac tgatccccgg ccagcccgga
     2221 aatcatcatc aggaaaccct tcaagatgac atggaagccg ttgacgcgct cgcaggagcg
     2281 ggcgttcatc ttgtgcggca tggcgctgga gccagtctgt cccttggcga acccctccgt
     2341 agccagttca tggccggcca tcaggcgcag ggtcttggcg aaggagg
//
LOCUS       DAOMSU010000325         2370 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4690_length_2370_cov_2.139093, whole genome shotgun sequence.
ACCESSION   DAOMSU010000325 DAOMSU010000000
VERSION     DAOMSU010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4690_length_2370_cov_2.139093"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PK077_10165"
     CDS             <1..1008
                     /locus_tag="PK077_10165"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRN24702.1"
                     /translation="FRSQMQALKASGVPVISMKEFLEWKLGDRQLPAKCVMITIDDGW
                     KSVYTDAYPILKETGFPFTIFPYTKFITGRGSAMSPAQIQEMLNNGATLGSHSVSHLY
                     PRSWRAAQRKGTQAVLELATAEIGNSRKILQEKFPGSSVEAYCYPGGFILPEMISKAE
                     EAGFQAAFTVIPKKVTKDTDRWRIHRYMVFGKDPKTFTRALNFNVPSTPETPAAPPGN
                     SSGKKLNSYPAPAQPVYPAANTAVKNQTPDISISLAGEPSLEPKQMEMRVSGFGLVNA
                     RYDAKEKILKWTPSRPLRLSPVTVQVRWKNPAVNMWQTATWQFGIEEQEMHFTPRNIV
                     K"
     gene            complement(1019..2341)
                     /locus_tag="PK077_10170"
     CDS             complement(1019..2341)
                     /locus_tag="PK077_10170"
                     /inference="COORDINATES: protein motif:HMM:NF039639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6056 family protein"
                     /protein_id="HRN24703.1"
                     /translation="MAVTFSVLFILYIISWFSPIAGDSFIHDRTGYLGQFHIRHVWKA
                     CVDSYLYWNPRLGEMAAFFITSAPRLVWTVLNPVFVLALVLGLYVLALGRMPNLRREC
                     GAWTWLFALSMFVSAGVTVYYVCLTRAGSMNYVWTGCLIVWFMNIYRTRWGKRITSSR
                     WGLSSGCLIYGIFCGACNEGATIGMAAAFCIMAAVGMFRDRRVGAYVWCGFAGVALGG
                     LFLFAAPGLYSRLGHDLGASEVLRKGGFCAYAESFGILLYYHARMLCKIYAVSAVLTG
                     FLLFSGTERCREVIRKEKESFFYILFLLLLGGIMSVCYFPVCIPAHHVQFVSSLPVLA
                     ASTVLFSVLYRNEIMVRGLRAVTVCLAGATLFFGVVFTLEYYHVGSQAFQREELIHKA
                     FQEGNLEPEVPSISPLFFEGKVSGTDGNWGGPDTNRRFGIRKLVVKKP"
BASE COUNT          673 a          734 c          543 g          420 t
ORIGIN      
        1 ttccgctccc agatgcaggc gctgaaggcg tccggagtgc ccgtcatctc catgaaggaa
       61 tttctggaat ggaaactggg ggacagacag cttccggcca aatgcgtcat gatcaccatc
      121 gacgacggct ggaagagcgt ctacacggac gcctatccca tcctgaaaga aacagggttc
      181 ccctttacca ttttcccata caccaaattc atcacgggcc gcggttctgc catgagcccc
      241 gcccagattc aggaaatgct gaataacggc gccaccctgg gcagccactc cgtcagccac
      301 ctgtacccaa gatcctggag ggccgcccag cggaaaggta cacaagcggt tctggagctg
      361 gcaacggcgg aaataggcaa ttcccggaaa atccttcagg aaaaattccc cggttcttcc
      421 gtggaggcct attgctatcc tggcggcttc atcctgccgg aaatgatctc caaggcggaa
      481 gaagccggat tccaggcggc ctttacggta atccccaaaa aagtgaccaa ggacacggac
      541 cgctggcgga ttcaccgcta catggtcttc ggcaaagacc ctaagacatt taccagagcc
      601 ctgaatttca acgttccctc cactccggaa actccggcgg ccccccctgg caacagcagc
      661 gggaaaaagc tgaattccta cccggccccg gcccagcccg tttatccggc cgccaatacc
      721 gcggtgaaaa accagactcc cgatatatcc atttccctgg caggggaacc ctccttggaa
      781 cccaagcaaa tggaaatgcg cgtctccggc ttcggcctgg taaacgcccg gtacgacgcc
      841 aaggaaaaaa ttttgaaatg gactccctcc cgtcccctgc ggctcagccc cgtaaccgtc
      901 caggtccgtt ggaaaaatcc tgctgtcaac atgtggcaaa cggccacctg gcaattcggc
      961 atcgaggaac aggaaatgca cttcactccc cggaacatag tcaaatagcc attttccctc
     1021 agggcttttt cactaccagc ttgcggatgc caaaccggcg gttggtatcc ggcccacccc
     1081 aattcccatc cgttccggat acttttcctt caaagaaaag aggagagatg gaaggaactt
     1141 ccggttccag attcccctcc tggaaggcct tatgaatcag ctcctctctc tggaaagcct
     1201 gagagcctac atgataatac tccagggtga acaccacccc gaaaaacaaa gtagctccgg
     1261 caaggcaaac agtcaccgcg cgcaatccgc gcaccatgat ctcattccgg tacaatacgg
     1321 agaacaacac ggttgacgcc gccaaaacgg gcagggacga cacgaactga acatgatggg
     1381 cgggaatgca caccggaaaa taacaaacgg acatgatgcc ccccagcaac aataaaaaaa
     1441 gaatataaaa gaaggattcc ttttccttcc tgattacttc ccggcaccgc tccgtgccgc
     1501 tgaacaacaa aaatcccgtt aatactgcgg aaacggcata tattttacac agcatcctcg
     1561 cgtggtaata aagcaatatc ccgaaagact ccgcataggc gcaaaaaccg cccttcctca
     1621 aaacttcact cgctcccaaa tcatggccaa gccgcgagta caacccgggg gcggcaaata
     1681 gaaacaggcc tcccaacgca acgccggcaa acccgcacca tacataagcg cccaccctgc
     1741 ggtcccggaa catgccaacc gcggccatga tgcaaaacgc cgccgccatt cctatcgtcg
     1801 ccccctcatt acaggcaccg cagaaaatcc cataaatcag acatcctgag gacaggcccc
     1861 agcgagagga ggttatccgc ttcccccacc tggttctgta aatattcatg aaccatacga
     1921 tcaggcatcc ggtccacaca taattcatgg agcctgcgcg tgtcaggcag acataataaa
     1981 cggttacccc ggctgagacg aacatgctta aagcaaacaa ccatgtccat gcgccgcact
     2041 cccgccggag attgggcatt ctccccagag cgagcacata aagaccaaga acaagggcaa
     2101 gcacaaacac gggattcaag accgtccaga ccagccgggg agcagaagta ataaaaaacg
     2161 cagccatttc ccccagcctg ggattccaat acaggtaact gtccacgcag gccttccaga
     2221 catggcggat atgaaactgc ccaaggtacc ccgtcctgtc gtgaataaag gaatccccgg
     2281 caatagggga aaaccatgaa ataatatata aaataaataa tactgaaaat gttacagcaa
     2341 tgggaaagac aaaaattctc ctcggcttca
//
LOCUS       DAOMSU010000326         2346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4752_length_2346_cov_2.197730, whole genome shotgun sequence.
ACCESSION   DAOMSU010000326 DAOMSU010000000
VERSION     DAOMSU010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4752_length_2346_cov_2.197730"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="PK077_10175"
     CDS             <1..706
                     /locus_tag="PK077_10175"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRN24704.1"
                     /translation="LNGRAALSAVCSHAIRRGWAERNPTAGVDIPVIKERRIAALPVQ
                     ACSLLLRLAGDMHHGECLAAAGIMLFAGIRPAEVERLRWKHVCLEDKVISIPPQHSKT
                     GGARHVSLQPALSSILKRVRRPADMPVTPANWKQKWRQVRARFSALTGRPWQTDVLRH
                     TFASYHAKQFRDFSSLQMDMGHSSLSLLRTRYLNMEGISRRESAAFWQLNHNALFIGP
                     SSSGSPEKTGTVPGIA"
     gene            880..1797
                     /locus_tag="PK077_10180"
     CDS             880..1797
                     /locus_tag="PK077_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRN24705.1"
                     /translation="MPSETLKGHIAMGAAAVIWGLMSPVSKLVMQQGEVSSASLATFR
                     LLGAAILFWLASAFVPGEPIERKHRLPLFYASLFGIIFNQMAFTVGVGFTSPADAAII
                     TTITPVLTMILAAFVLREMVTGKKIIGVCASAIGAILLVSSSGGRAAALPGDNNLLGD
                     ILCLASQCSVAVYFVFFKNLIGRYSPVTLLKWMFTYAAAVCLPFTFREVGSIHYSALP
                     AQTWLGIAYVVALATFVSYICLSFAQQRLKPTAVSMYNYCQPVIASSVAVMWGMDHFG
                     WMKVLSVLLVFTGVLLVTGTGGKRAVQEQ"
     gene            2019..>2346
                     /locus_tag="PK077_10185"
     CDS             2019..>2346
                     /locus_tag="PK077_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24706.1"
                     /translation="MNRFFLSWDKPACRAVAERLLSLGNDFHRHLVLVPTRESGRQLR
                     EFLASISHTQAIFAPQVIPADQFPRMEEKEETASAPEELAGWLLALGDSPHRLYPRLF
                     PRPMPED"
BASE COUNT          459 a          778 c          628 g          481 t
ORIGIN      
        1 tctgaatggc cgggcggccc tcagcgcggt ctgctcccac gccatacgcc gcggctgggc
       61 ggaacgcaac ccaacggcgg gggtggatat ccccgtcatc aaggaacgcc gcattgccgc
      121 cctgcccgta caggcatgct ccctgctgct gcgcctggcc ggggacatgc accacgggga
      181 atgtctggcg gcggcaggca tcatgctgtt tgcgggaatc cgcccggcgg aagtggagcg
      241 gctccgctgg aaacacgtct gcctggaaga caaggtaatc tccatcccgc cgcaacactc
      301 caaaacgggc ggggcgcgcc atgtcagcct ccagcccgcg ctgtccagca tcctgaaacg
      361 cgtcaggagg cccgcagaca tgcccgtcac ccccgccaac tggaaacaaa aatggcgaca
      421 ggtcagggcg cgtttctccg ccctgaccgg caggccatgg cagacggacg tgctccgcca
      481 caccttcgcc agctaccacg ccaagcaatt cagggatttt tcctccctgc aaatggacat
      541 ggggcactcc agcctgtctc tgcttcgcac gcgttatctc aatatggagg ggatttcacg
      601 ccgggaatcc gcagccttct ggcaattaaa ccacaacgct ctcttcatcg ggccttcttc
      661 atccggctct ccggaaaaaa cgggaacagt tcccggcatc gcctgaagcc ggaaccatgt
      721 ctgccgcgtt gactcccata ttcattccag gcgcagaatc atctgacgga cagagaaagc
      781 attttccgtt ttttatcttt gccgggaaca cccataaaac tcttgcaatc cgcttgcgga
      841 agcggagaat gtgcgacccg ccgcgcagcg ggccgcacca tgccgtcaga aacgctgaaa
      901 ggacatatcg cgatgggagc ggcggccgtc atctggggcc tcatgtctcc cgtcagtaaa
      961 ctggtcatgc agcagggaga agtaagttcc gcttctctcg ccacattccg cctgctggga
     1021 gcagccatcc tgttctggct ggcctccgct ttcgtgccag gggaacctat agaaagaaag
     1081 caccgcctgc ccctgtttta tgcctccctg ttcggaatca tcttcaacca gatggccttc
     1141 acggtgggcg tagggttcac ttccccggcg gatgcggcca tcatcaccac cattacgccg
     1201 gtactgacca tgattctggc ggccttcgtc ctccgggaaa tggttacggg gaaaaaaatc
     1261 atcggggtct gcgccagcgc catcggagcc attctgctgg tttccagcag cggagggcgt
     1321 gcggcagccc tcccgggaga caacaacctg ctgggggaca ttctctgcct ggcttcccag
     1381 tgcagcgtag ccgtttactt tgtcttcttt aagaacctga ttggcagata ctcgcccgtc
     1441 accctgctga aatggatgtt tacgtatgcc gccgccgtct gcctcccctt caccttccgg
     1501 gaagtggggt ccatccacta ttccgccctc cccgcacaaa catggctggg cattgcctac
     1561 gtggtggctc tggctacctt tgtcagctat atctgcctgt ctttcgccca gcagcggctg
     1621 aaacccaccg ccgtcagcat gtacaattac tgccagccgg tgattgcctc atccgtggcg
     1681 gtgatgtggg gcatggacca cttcggatgg atgaaagtcc tctccgtcct gctggtcttt
     1741 acaggcgtgc ttctggtcac cggaaccggg ggaaaacggg ccgtccagga acaataaaca
     1801 cgccattcct ccggatttcc gccgccacgc cctaccggaa aggcaaaaaa gttcctccct
     1861 tctccgccaa catgcggcca aagcccgctc acctcacggc ccgctatgcg ccgccccgtt
     1921 aatcatgaaa aaatagatgg gaaattcagc cattctgata tagcattccc atgtagccct
     1981 caacttttcc cctttccggg gattcctgct tccctgccat gaaccgattc tttctatcct
     2041 gggacaaacc ggcctgccgg gccgtggcgg aacgcctgct ttccctggga aatgacttcc
     2101 accggcatct ggtgctggtt cccacacggg aatccgggag gcagctgagg gaattcctcg
     2161 cctccatttc ccatacacag gcaatcttcg cgccccaggt cattccggca gaccagttcc
     2221 cccggatgga agaaaaggaa gaaacggcat ccgctccgga agaactggcc gggtggctcc
     2281 tggctctggg ggactctccc caccgccttt atccccgcct gtttccccgc cccatgccgg
     2341 aggatt
//
LOCUS       DAOMSU010000327         2336 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4776_length_2336_cov_2.498904, whole genome shotgun sequence.
ACCESSION   DAOMSU010000327 DAOMSU010000000
VERSION     DAOMSU010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2336
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4776_length_2336_cov_2.498904"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..153)
                     /locus_tag="PK077_10190"
     CDS             complement(<1..153)
                     /locus_tag="PK077_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRN24707.1"
                     /translation="MNQYALILAGGSGTRFWPLSRDHRPKQLLNMFGEGTLLRQAIDR
                     LDGLVPR"
     gene            complement(186..950)
                     /locus_tag="PK077_10195"
     CDS             complement(186..950)
                     /locus_tag="PK077_10195"
                     /inference="COORDINATES: protein motif:HMM:NF011405.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRN24708.1"
                     /translation="MLNWIRSWTRVKQRLSMLTIPKEKLPIHIACIMDGNGRWANSRH
                     LPRQAGHRAGADTVERCADFCMTHNIPWLTLYAFSSENWNRPKTEVHALMLLLHEFLK
                     KRLHQMMEKGVRLHAIGDLSRLPARTRKLLQDSLEATSGNTRLNLVLAISYGSRGEIA
                     SAARKIAEKVSRGELQPDAVTEDLFGEYLDTAGMPEPDLLIRTSGEMRVSNFLLWQIS
                     YAEIHVTDTLWPDFGDRDMEAALLDYSRRKRRFGAL"
     gene            complement(922..>2336)
                     /locus_tag="PK077_10200"
     CDS             complement(922..>2336)
                     /locus_tag="PK077_10200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRN24709.1"
                     /translation="EIPKGRKDGATEEPNGTRTAFHADPDIFPAATQFRPEYIEKMCR
                     YYSYLNKGLALHFNGRRYISKNGLLDLLAEAMSEEPLYPIIHLEGHDIEVAFTHGGQY
                     GEEHYSFVNGQHTTQGGTHQAAFREAIVKTLRDFFKKNYEAGDIRSSLVAAISIKVKE
                     PVFESQTKTKLGSNTISPEGETIRTFVGNFIARELDNYLHKNPETAKALEAKIKDSER
                     DRKELSGIQKLARENARKAKIHNKNLRDCRVHYNSKHSRAGETTLFITEGISASGSIT
                     TARDAETQAVFSLRGKPLNSFGMSRKVVYENEEFNFLQHALNIENGLEELRYDKVVIA
                     TDADDDGMHIRILLMTFFIQFFPELIREGHLFILQTPLFRVRNKQQTIYCYSDAEREE
                     AIGLLGKNPEITRFKGLGEISPQEFKAFIGEGMRLDRVRLEDSHKVKDLLSFYMGKNT
                     RERQEYILNNLRVELDPVMD"
BASE COUNT          418 a          608 c          731 g          579 t
ORIGIN      
        1 ccggggcacc agcccgtcca gcctgtcgat ggcctggcgg aggagggttc cttccccgaa
       61 catgttcagg agctgtttgg ggcggtggtc gcgggagagg ggccagaaac gggtgccgct
      121 gccgccggcc aggataagag cgtattggtt catgtctgtt tcgcaggaat gggggttaaa
      181 aaaggtcaga gcgctccgaa acggcgtttt ctgcgggagt agtccagcag agcggcttcc
      241 atgtcccggt cgccaaagtc cggccacaat gtgtccgtca catgaatttc cgcgtagctg
      301 atttgccaga gaagaaagtt ggagacgcgc atttctccgg aggtgcggat aagcaggtcc
      361 ggttccggca tgccggcggt gtccagatac tcgccgaaca ggtcttccgt cacggcatcc
      421 ggctggagct ccccgcggga gactttttcc gctattttcc tggcggcgga ggcaatttcc
      481 ccacggctgc cgtaggaaat ggccagaacc aggttgagcc tggtgtttcc cgaagtggct
      541 tccagactgt cctgaagcag tttccgggtg cgggcgggaa gacgggaaag gtcgcctatg
      601 gcatgaaggc gcacgccttt ttccatcatt tgatggagac gttttttcag gaattcgtgc
      661 agcaggagca ttaacgcgtg cacttccgtc ttcgggcggt tccagttttc cgaggaaaaa
      721 gcgtaaagag tgagccaggg aatattatgg gtcatgcaga agtccgcaca gcgctccacc
      781 gtatccgctc ccgcccggtg gcctgcctgc cgcggcagat gacggctgtt cgcccaacgg
      841 ccgttgccgt ccatgatgca ggcgatgtga atcggcagtt tttccttggg aatggtaagc
      901 atagataggc gttgctttac tctagtccat gaccggatcc agttcaacac gcaagttgtt
      961 caggatgtat tcctgtcttt cccgcgtgtt tttgcccatg tagaaggaca gcaggtcttt
     1021 caccttgtgg gagtcttcca gacggacgcg gtccaggcgc atcccttccc cgatgaaagc
     1081 cttgaattcc tggggggaga tttctcccag gcctttgaac cgggtaattt ccgggttttt
     1141 ccccagcagg ccgatggctt cctccctttc tgcgtccgag tagcagtaga tcgtttgctg
     1201 tttgttgcgc acgcggaaga ggggcgtctg gaggatgaaa aggtgtccct cccgtatcaa
     1261 ttccggaaag aactggatga agaatgtcat cagcaggata cggatgtgca tgccgtcgtc
     1321 atcagcgtcc gtggcgatga ccactttgtc atagcgcagt tcctccagcc cgttttcaat
     1381 gttcagggca tgctggagga agttgaattc ctcattttca tagacgactt tacggctcat
     1441 gccgaaggag ttcagcggct tgccccgcag ggagaaaacg gcctgggttt ccgcgtcgcg
     1501 cgccgtcgtg atagagccgg aggcggagat gccttccgta atgaagaggg ttgtttcccc
     1561 ggcgcgggag tgcttggagt tgtaatgcac ccggcagtcc cggagattct tgttgtgaat
     1621 tttggccttg cgggcgtttt cgcgcgccag tttctggatg ccggagagtt ccttgcggtc
     1681 ccgttcggaa tccttaatct tggcttccag ggctttggcc gtttccgggt ttttgtgcag
     1741 gtagttgtcc agctccctgg caatgaagtt gcccacaaag gtgcggatgg tttccccttc
     1801 cgggctgatg gtgttggacc ccagcttggt tttggtctgg gattcgaaga cgggttcctt
     1861 taccttgatg gagatggcgg ctacgaggga ggagcggatg tcccctgcct cataattttt
     1921 tttgaagaag tccctcaggg ttttgacgat ggcttcccgg aaggccgcct ggtgggtgcc
     1981 tccctgggtg gtgtgctgcc cgttgacgaa ggagtagtgc tcttccccgt attggccgcc
     2041 atgggtgaag gccacttcaa tgtcatgccc ttccagatgg atgatagggt acaatggctc
     2101 ctcgctcatc gcttccgcaa gcagatccag caacccgttt ttggagatgt agcggcggcc
     2161 gttgaaatgc agggccagcc ctttattgag gtaggagtag tatctgcaca ttttttcgat
     2221 atactccggc cggaattgcg tggccgcggg aaagatgtcc ggatccgcgt gaaacgccgt
     2281 gcgggtgccg ttgggttcct ccgtggcgcc gtccttgcgt cccttgggga tttcct
//
LOCUS       DAOMSU010000328         2334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4782_length_2334_cov_1.885476, whole genome shotgun sequence.
ACCESSION   DAOMSU010000328 DAOMSU010000000
VERSION     DAOMSU010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4782_length_2334_cov_1.885476"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>317
                     /locus_tag="PK077_10205"
     CDS             <1..>317
                     /locus_tag="PK077_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRN24710.1"
                     /translation="FTYTADDLMEFFSGKDIGTLLLVNPGNPSGFFLPKGDVLRLCQW
                     TKTRGVRLIVDESFVDFSCGMPDNTLLKDGILEEYPHLAVVKSISKSYGVPGLRLGVL
                     ASA"
     assembly_gap    318..327
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <328..707
                     /locus_tag="PK077_10210"
     CDS             <328..707
                     /locus_tag="PK077_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRN24711.1"
                     /translation="DVSIWNINSIAEFYMQIFGKYEQSYVRTCNRFMKERDRFMQELS
                     RVPFLNVFPSQANYFMCEVKPPMKARSLTGLLLKNHSILVKDCSRKKGFEGREFIRIA
                     VRDATDNDALVRAMHELCSMHSN"
     gene            757..1716
                     /locus_tag="PK077_10215"
     CDS             757..1716
                     /locus_tag="PK077_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24712.1"
                     /translation="MLTIPHEIISSLNISPRQSIQWVKESFHIKEEEPVFLPHKTSIA
                     FEEGKFMNTMPCIVPGLNAMGVKIVTRYPERAKTINGEILLYDYHTGELLALMDAFWV
                     TNARTGAVAALALQTFARQDFQTISLMGLGNTARATMACILALYPEKNFTVRLLSYKT
                     HVPQFIEEFQCHSNVRFEEVKNINNLIDGTDAVISCITNAEGILAEDSCFKPGITVIP
                     VHTKGFQNCDLFFDKVFADDRDHVKNFRHFERFRQFGEIAPVLAGKTAGRTSPEERIL
                     SYNIGLGLHDIVFAKHIYDRLIHVEEQKEKSPSFHFRQTACLS"
     gene            complement(1717..>2334)
                     /locus_tag="PK077_10220"
     CDS             complement(1717..>2334)
                     /locus_tag="PK077_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HRN24713.1"
                     /translation="ALKTAFCPAFQQLTSLAGSGVIGSIKAVDATFTKLIEDEAAREY
                     DPMQAGGAWTELGSYPAFVIGKLLGTDPRRVRFVTCRNSHTGVDVFTRAEFLYSNAVA
                     TATAAIGAKQEGDLCITGTEGYIYVPAPWWKTEMFEVRFEDSRLNRKYFANFEGDGLR
                     YELGVFLRLIHGCHHGNRLLTREDSMFMADVASRFRRGECVDEIS"
BASE COUNT          593 a          673 c          518 g          540 t
ORIGIN      
        1 atttcactta cacggcggac gacctgatgg aattcttttc cgggaaagac atcggtaccc
       61 tgctcctcgt caatccggga aacccttccg gcttcttcct gccgaaagga gatgtgctgc
      121 gcctctgcca gtggacaaaa acccgcggcg tccgcctgat cgtggatgaa tcctttgtag
      181 acttctcctg cggaatgccg gacaatacgc tgctgaaaga cggcatcctg gaagaatatc
      241 cccacctggc cgtggtaaaa agcatttcca aatcctacgg cgttccgggc ctccgcctgg
      301 gtgttctggc ctccgcgnnn nnnnnnnaag acgtttccat ctggaacatc aattccattg
      361 cggaatttta catgcaaatc ttcgggaaat atgagcaatc ctacgtgcgt acgtgcaacc
      421 gtttcatgaa agaacgggac cgcttcatgc aggaactctc ccgcgtgcct ttcctgaacg
      481 tgttcccctc ccaggcaaac tatttcatgt gcgaggtcaa gccccccatg aaagcccgct
      541 ccctgaccgg ccttctgctg aaaaaccact ccatccttgt taaggattgc agccggaaaa
      601 aaggatttga aggcagggaa ttcatccgta tcgccgtcag agacgcaacc gacaacgatg
      661 ctctggtccg ggccatgcac gagctctgtt ccatgcattc aaactagtca ttaactctct
      721 ttttccaaac gccgttttta ctctaatccc cctaccatgc tgacaattcc tcacgaaatt
      781 atttcctccc tcaatatttc tccccggcag tcaatccaat gggtaaaaga gtccttccat
      841 atcaaggagg aagaacctgt attccttccc cataaaacaa gcatcgcctt tgaagaagga
      901 aaattcatga atacgatgcc ctgcatcgtc cccggcctga acgccatggg ggtaaaaatt
      961 gtaacgcgtt acccggaacg ggccaaaacc attaatgggg aaatactact ttacgattat
     1021 catactggag agcttctggc tcttatggac gctttttggg tcacgaacgc ccgtaccgga
     1081 gcagtagccg ccctcgccct tcaaacattc gccagacagg atttccagac aatttccctg
     1141 atggggctgg gaaatacggc ccgcgccact atggcatgca ttctggcgtt atatcctgaa
     1201 aaaaacttca cggtgcgtct tctttcctac aaaacgcatg ttccccaatt cattgaagaa
     1261 ttccaatgcc acagcaacgt ccgttttgaa gaagtgaaaa acatcaataa cctcattgat
     1321 ggaacagatg ccgtcatttc ctgcattact aatgcggagg gcatccttgc cgaagacagc
     1381 tgtttcaaac caggcattac tgttatcccc gtacatacaa agggattcca aaattgtgat
     1441 ttgttttttg acaaggtatt tgccgatgat cgtgaccatg ttaaaaattt ccggcatttt
     1501 gagcgcttcc gtcaatttgg ggaaatagct ccggttctgg ccggaaaaac agcgggaaga
     1561 acgtctccgg aagaacgcat cctcagctac aatatcgggc tgggcctcca cgatatcgtc
     1621 ttcgccaagc acatctacga ccgcctgatt catgttgagg agcagaagga aaaatctccc
     1681 tcctttcatt tccggcaaac ggcatgcctg tcctgatcag ctgatttcat caacacattc
     1741 cccacgcctg aagcgggagg caacatcagc catgaacatg gaatcctcac gggtcaaaag
     1801 acggttgcca tgatgacagc cgtgaatcag gcgcaaaaac acgccgagct cataacgcag
     1861 cccgtcccct tcaaaattgg caaaatactt cctgttgagc cgggaatcct caaaccgcac
     1921 ttcaaacatc tccgtcttcc accacggcgc cggcacataa atatacccct ccgttccggt
     1981 aatgcataag tccccttcct gcttggctcc tatggctgcc gtagcggtgg ctactgcatt
     2041 ggaataaaga aattccgcgc gcgtgaacac atccacgccc gtatgagaat ttctgcaagt
     2101 cacaaaacgg accctgcggg gatcggttcc cagaagcttc ccaatgacaa aggcgggata
     2161 ggaacccagt tccgtccagg cgcctccggc ctgcatgggg tcatactccc tggcagcctc
     2221 atcctctatc agcttggtaa acgtggcgtc caccgcctta atggaaccaa tgacgccgct
     2281 gcccgccaaa ctggtcaact gctggaaagc cgggcaaaac gcagttttaa gcgc
//
LOCUS       DAOMSU010000329         2327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4803_length_2327_cov_2.819542, whole genome shotgun sequence.
ACCESSION   DAOMSU010000329 DAOMSU010000000
VERSION     DAOMSU010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4803_length_2327_cov_2.819542"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /gene="mnmE"
                     /locus_tag="PK077_10225"
     CDS             complement(<1..954)
                     /gene="mnmE"
                     /locus_tag="PK077_10225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HRN24714.1"
                     /translation="MTDPERTIAAQATAAGQGAIAVIRMSGPGCMNILKQCTPAAFTE
                     RLRPRRATLVCIRDAEGAVIDQALVTWFPAPASYTGEDTAEISCHGGMLVTDRLLKRL
                     YQCGAFPAEPGEFTKRAFLNGRMDLTQAEAVMDVISAGSDLALKAAQTQLDGGVGAQV
                     DELKDNLVHVLAHIEAYIDFPDEDISPDTASDLLARLQSMEEKLAALLRTAEGGRLLR
                     EGIRTAIAGPPNVGKSSLLNTLLGYDRAIVSNIAGTTRDTVEESIQMAGLALRLIDTA
                     GMRESSDVIEQAGITRTNRALETADLVLEVADASMPRVKDHT"
     gene            complement(951..2024)
                     /gene="mnmA"
                     /locus_tag="PK077_10230"
     CDS             complement(951..2024)
                     /gene="mnmA"
                     /locus_tag="PK077_10230"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRN24715.1"
                     /translation="MARILVGLSGGVDSSVAAALLVEQGHDVVGAYMKNWVNDEGIPG
                     ECPWEQDIQDALAVAKTTGIEFRVIDLVDEYRARIVNYLIEGYRAGYTPNPDVLCNRE
                     MKFGVFLDYALEQGFDYVATGHYARRMDTPQGAFILRGRDPNKDQSYFLSLMRPDQIA
                     RAVFPLGDLLKPEVRVLAEKYGLPTARKKDSQGICFIGQVKMSDFLRHYLPDKPGKIV
                     DTEGRTLGTHNGLHLFTMGQRKGHGVASPREGVAYVVVGKDVQRNRLILGYEDASTRG
                     LYASRAVVSGISNTLSPLPARVMAQPRYRAKAEWASCEYLEEGKVRLGFDTPLRALAV
                     GQVCAFYDGGKLLGGGFFESIEP"
BASE COUNT          428 a          690 c          704 g          505 t
ORIGIN      
        1 ggtgtggtct tttacccggg gcatggaggc atccgccact tccagaacca gatccgcggt
       61 ttcaagcgcc ctgttggtgc gggtaatgcc agcctgttca atgacgtcgg aagattctcg
      121 catgcctgcc gtatcaatca gcctcagagc cagtccggcc atctggatgg attcctccac
      181 cgtgtcccgc gtggttccgg caatattgct gacaatggcc cggtcatatc ccagcagggt
      241 attcagcagg ctggatttac ccacattggg aggtccggcg atcgccgtgc ggatgccttc
      301 ccgcagcagg cgtccgcctt cggcagttct gaggagggcg gccagttttt cctccatgct
      361 ctggaggcgc gccagaagat cggaggcggt gtccggggaa atgtcttcat ccggaaaatc
      421 aatataggcc tcaatgtggg ccagcacatg aaccagattg tctttcagtt cgtccacctg
      481 ggcgccgacg cctccgtcca gctgggtttg cgcggctttc agagccaggt cgcttccggc
      541 ggaaatcaca tccatcacgg cttccgcctg ggtcaggtcc atgcgcccgt tcaggaaggc
      601 cctttttgtg aactctcccg gttccgcagg gaaggccccg cattggtaaa ggcgtttcag
      661 caggcggtcc gtcaccagca tgccgccatg gcaggagatt tccgcggtgt cttcccccgt
      721 atagctggcc ggggcgggga accaggtgac aagcgcctgg tcaatgacgg ctccttccgc
      781 gtcccggatg cagaccagcg tcgccctgcg cggccggagg cgttccgtga aagccgcagg
      841 ggtgcattgt ttcaggatat tcatgcagcc ggggccgctc atgcggatga cggcgatggc
      901 tccctgtcct gcggcggtgg cctgtgcggc tatggtgcgt tccgggtcag tcatggttct
      961 atggattcaa aaaagccgcc cccgagcaac ttgcctccgt cgtaaaaggc gcagacctgt
     1021 cccacggcca gggcccggag gggcgtgtca aagcccaggc gcacctttcc ttcttccaga
     1081 tattcgcagg aggcccattc cgccttggcc cggtagcggg gctgggccat gacgcgggcc
     1141 ggcagggggg agagagtatt ggaaatgccg ctgaccacgg cgcgggacgc gtacaatccc
     1201 cgcgtggatg cgtcttcgta tcccagaatc aggcggttgc gctggacatc ctttcccacc
     1261 accacatagg cgactccctc ccgcggggag gcgactccgt gccctttcct ctggcccatg
     1321 gtgaacagat gcaggccgtt gtgcgtgccg agcgtcctgc cttccgtgtc cacgattttg
     1381 ccgggcttgt ccggcaggta gtggcgcagg aaatcgctca tttttacctg gccgatgaag
     1441 cagatgccct ggctgtcttt cttgcgtgct gtggggaggc cgtatttttc cgcaagaacg
     1501 cgcacttccg gtttcagcag atccccaagg ggaaagacgg cgcgggctat ctggtccggg
     1561 cgcatcaggg acaggaagta ggactggtcc ttgttcgggt cccgcccgcg caggatgaaa
     1621 gccccttgcg gcgtgtccat cctgcgggcg taatggccgg tggcgacgta gtcgaacccc
     1681 tgttccaggg cataatccag aaagacgccg aatttcattt cccggttgca caatacgtcc
     1741 gggttgggcg tatatccggc gcggtagcct tcaatcagat aattcacgat gcgggcgcgg
     1801 tattcgtcca ccaggtcaat gacccggaat tcaatacctg ttgttttggc gacggccagc
     1861 gcgtcctgaa tgtcctgttc ccacgggcat tcgccgggaa tgccttcgtc attcacccag
     1921 tttttcatgt aggcccccac tacgtcatga ccctgctcca cgagcagggc cgctgccacg
     1981 gagctgtcca ctccgcctga taagcctaca aggatgcgtg ccacgatgct ggaaaaaata
     2041 tggggtgata gggcggaccg ctttgagaaa ggctttgccg cctgcccctg taaaccaaag
     2101 atgtttccgt aagccaagac aaaaagcgtg gaaatggggc atccggtccc tgatgcggaa
     2161 tatgattcgg tcgatccggg aggcttttga tttttaatca cattccctga aatcgaattt
     2221 ggaattcgct atgacaggtt cattcgcgcg ctgatgaatc atgggaggaa cgatggattc
     2281 gtccccctgc cattcaccgg ttgcgatagt tacaacccca tcagaat
//
LOCUS       DAOMSU010000330         2294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4905_length_2294_cov_3.022331, whole genome shotgun sequence.
ACCESSION   DAOMSU010000330 DAOMSU010000000
VERSION     DAOMSU010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4905_length_2294_cov_3.022331"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="PK077_10235"
     CDS             <1..941
                     /locus_tag="PK077_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRN24716.1"
                     /translation="APQDGSVVDIDGSDKCMAMACAILPEWGKTDPYAMGTGTVDECV
                     RQLILVGSNPDKIGLLDNFCMGNPEDPAELGRLVECVKAIAKAADAYNAPFISGKDSF
                     YNYFETEDGPINVPVTFLCSGFGVVENPDHVIGSSLRRSNSLLYLIGHTEDEMGGSVF
                     ARTHGVEDGKVPQTDCAANMELYKAYYSALTSGLVLSAHDVSEGGLAVTAAEMAFSGK
                     GGVRLDLTKVPTANGWKSPAVPLFSESTGRILVEVDPEFAADFETAMDGFPCACIGGV
                     TEEKRLTATCCGGEKVLDCDIANLKKLWKDGLTPYY"
     gene            990..1697
                     /locus_tag="PK077_10240"
     CDS             990..1697
                     /locus_tag="PK077_10240"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HRN24717.1"
                     /translation="MPHALLLKYPGTNCDVETERALRAAGFSTQIQPIATATPEHVAK
                     SQLVVLGGGFSYGDYVTSGRLAQLETERFLGDALQRHHAGGGMLLGICNGFQILTKLG
                     ILPDSSLIDNKKERFECVWAKLVKVARNSPFLQGLPDEFELPSAHAEGRLVTRPGDAE
                     KYLAAGNVALQYVDNRDGCECSIAGLQDSTGRAMGLMPHPERFLLPRHHYDKDWAGNE
                     DWGWGYFLFKSAFDALK"
     gene            1739..>2294
                     /locus_tag="PK077_10245"
     CDS             1739..>2294
                     /locus_tag="PK077_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24718.1"
                     /translation="MTDTALIGFSCALGVFWSSKRYFDYLIDETNLSLMRGKEFGQAV
                     IICPSLREGSTAIRRNTAPFLAQVKTLIDLLHEVKAERVTYITSIDTQPETGNELSPL
                     LREPEDEWLAALVELKDFINLRFGRVLNVYLPEVTGTGAGMSVADLLAEVPEGTEELD
                     VALLERHQLYPMHRLVRDVEKAWEC"
BASE COUNT          483 a          707 c          654 g          450 t
ORIGIN      
        1 acgccccgca ggacggttcc gtagtggaca tcgacggctc ggacaaatgc atggccatgg
       61 cttgcgccat tcttccggaa tggggcaaaa cggatcccta cgccatgggt accggcaccg
      121 tggatgaatg tgtgcgccag cttatcctgg ttggctccaa tccggacaaa atcggcctgc
      181 tggacaactt ctgcatgggt aatccggaag atccggcgga actcggccgc ctggtggaat
      241 gcgtgaaagc catcgccaag gcggcggacg cgtacaatgc ccccttcatt tccggcaagg
      301 actccttcta caactacttt gaaacggaag acggccccat caacgtgccc gtcaccttcc
      361 tctgctccgg cttcggcgtg gtggaaaacc cggaccacgt catcggttct tccctgagga
      421 ggagcaacag cctgctgtac ctcatcggcc atacggaaga tgaaatgggc ggctccgtct
      481 tcgcccgcac ccacggggtg gaagacggca aggttcccca gacggactgc gccgcgaaca
      541 tggagctgta caaagcctac tacagcgccc tgacttccgg tctggtgctc tccgcccatg
      601 acgtttccga aggcggcctg gccgtcacgg ctgcagaaat ggccttttcc ggcaagggtg
      661 gcgttcggct ggatctcacg aaggtaccga ctgccaatgg ctggaaatcc ccggcagtgc
      721 ctctgttcag cgaaagcacg ggccgtatcc ttgtggaggt ggatccggaa ttcgccgcgg
      781 attttgaaac ggctatggac ggcttcccct gcgcctgcat cggcggcgtt acggaagaaa
      841 agcgcctcac ggccacctgc tgcggcgggg aaaaggtgct ggattgcgac atagccaacc
      901 tcaaaaagct ctggaaggac ggacttaccc cctattattg atcccctgaa accggcatct
      961 taccaaacca acgattcatt tctaccaata tgcctcacgc acttttactg aaataccccg
     1021 gcaccaactg cgacgtggaa acggaacgcg ccctgcgcgc ggcagggttc tccacccaga
     1081 tccagcccat cgccacggct acgccggaac acgtcgcaaa atcccagctt gtcgtcctgg
     1141 gcggcgggtt cagctacggg gactacgtca ccagcggccg cctggcccag ctggaaacgg
     1201 aacgcttcct gggggatgcc ctgcagcggc atcatgccgg aggaggcatg ctcctgggca
     1261 tctgcaacgg gttccagatt ctcaccaaac tgggcattct tcctgactcc agcctgattg
     1321 acaataaaaa ggaacgcttt gaatgcgtct gggccaaact ggtgaaagta gccaggaact
     1381 ctcccttcct tcaaggcctg ccggatgaat tcgaactgcc ctccgcccat gcggaagggc
     1441 gcctggtcac ccgccccgga gatgcggaaa aatacctggc tgccggaaat gtggctctcc
     1501 agtacgtaga caaccgcgac ggctgtgaat gcagcatcgc cggcctccag gattccaccg
     1561 ggcgcgccat gggtctgatg ccccatccgg aacgctttct gctgccccgc caccactatg
     1621 acaaggactg ggccggcaac gaagactggg gctgggggta tttcctcttc aaatccgcct
     1681 ttgacgccct gaaataaagg ttcattcccc atcaaccgtt tccccccgca ttcattccat
     1741 gacagatacg gccctcatcg gtttttcttg cgccctcggc gttttctggt cctcaaaacg
     1801 gtactttgac tacctgattg atgaaaccaa cctgtccctg atgcgcggca aggaattcgg
     1861 gcaggccgtc attatctgcc cctccctccg ggaagggagc acggctatcc ggcggaatac
     1921 ggctcccttt ctggctcagg ttaaaaccct gattgatctc ctgcatgagg tgaaggcgga
     1981 acgggtgacc tacatcacca gcattgacac ccagccggaa acgggaaatg aactttctcc
     2041 cttgctccgt gaaccggagg acgaatggct ggcggctctg gtggagctga aagacttcat
     2101 caacctccgg ttcggccgcg tgctgaacgt ttaccttccg gaagtcaccg gaacgggagc
     2161 aggaatgagt gtggcggatc tgctggcgga ggttccggag ggaacggaag agctggacgt
     2221 ggctctgctg gagcgccatc agctttatcc catgcaccgc ctggtcaggg acgtggaaaa
     2281 agcctgggag tgcg
//
LOCUS       DAOMSU010000331         2277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4959_length_2277_cov_1.890189, whole genome shotgun sequence.
ACCESSION   DAOMSU010000331 DAOMSU010000000
VERSION     DAOMSU010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4959_length_2277_cov_1.890189"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="PK077_10250"
     CDS             <1..906
                     /locus_tag="PK077_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24719.1"
                     /translation="TQPEAQASARPAISGKTGTTSFTTALPPFALRYAWLWPFRKNSD
                     KRQGAAPGPQPAIQGKTWEACMAEAREKLGQGGGYACTGKALDNFIQAFSRDERKGLR
                     LQPGKARPSFCSSAVYGALLLALELWNSGREENGLDGNAWQALAPRKAPDGTGAWGCA
                     NANGPGLAVLIHKLGGGHSFTEWEKARSGDIMKLWWTEAVGAKERGHLTIYLSQTEDS
                     LTFWSSNQSNSGKTPGYGTKTIPKSTVRHVLFTRITRPEAFANAPHVGTDPWLGSLLK
                     KTVTMEEALSRCGLTPSPSTKTIQP"
     gene            903..1388
                     /locus_tag="PK077_10255"
     CDS             903..1388
                     /locus_tag="PK077_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24720.1"
                     /translation="MNYPPPSPMPRLSIPALVWSGCCAIAGITLIVYTCQHYAQWSKA
                     LPGSGELRAQVEHARKIRLRHFSLQLEWQEKSIGMALTEQQMAKLKEETSQIKREIWE
                     EESAWKNLAEQIDRQQTALLQAQAEMQFAKSALRAAGVRQKDIENRISSLENELQEKS
                     R"
     gene            1402..1809
                     /locus_tag="PK077_10260"
     CDS             1402..1809
                     /locus_tag="PK077_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24721.1"
                     /translation="MKRLTIYSTLFLALCRSAWGLSWYALTTLRSETRSMNRELSFFN
                     EQEERYQKTIPQRIVDEKRFRQFPSTIEKFREMAVQHRQIAGRRQAVADGVFQKYLDK
                     ILASAKEREKRIILLQRITKQLEQEAQQLTHKR"
     gene            1824..>2277
                     /locus_tag="PK077_10265"
     CDS             1824..>2277
                     /locus_tag="PK077_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24722.1"
                     /translation="MKTILASVCLALSLLLPARAAGYDILLPWLAPQRLGFTSGKAIL
                     DFAEDKKRSELDMLVLDKTRSLPADIANAFNKITTPVAASFLAYSRLHNKTVDENNFF
                     SKGIAWSYLVIRLHQADKDNNQTGYVTGWYTNANGSDYRTYGAFESVSR"
BASE COUNT          584 a          695 c          582 g          416 t
ORIGIN      
        1 acgcagccgg aggcgcaggc gtccgcccgg ccagccatat caggcaaaac cggaaccacc
       61 tccttcacca ccgctctccc gccgtttgcg ctgcgttacg cctggttatg gcccttccgg
      121 aaaaattcgg ataagaggca gggggcggct cccggccccc aacccgccat acagggaaaa
      181 acatgggagg catgcatggc ggaggccagg gaaaaactgg gacagggcgg tggatatgcc
      241 tgcacgggca aagcgctgga caatttcatc caggcgtttt cccgggatga acggaaaggc
      301 ctccggctgc agcccggaaa agcgcggcct tccttctgtt cttccgccgt ttacggcgcg
      361 ctgctcctgg cgctggagct ctggaactcc ggcagggaag aaaacggcct ggacgggaac
      421 gcctggcagg ccctggcccc cagaaaagcc ccggacggca cgggggcctg gggctgcgcc
      481 aacgccaacg gccccggcct ggccgtccta atccataagc tgggaggcgg gcacagcttt
      541 accgaatggg aaaaagcccg ctccggagac atcatgaaac tgtggtggac ggaagccgtc
      601 ggcgcgaagg aacgcggcca tttgaccatc tatctctccc agacggaaga cagcctgacc
      661 ttctggtcct ccaaccagtc caactccggg aaaacgccgg gctacggaac caaaaccatc
      721 cccaagtcaa ccgtcagaca cgtcctcttt acccgcatta ccaggccgga agccttcgcg
      781 aacgcccccc atgtcggaac ggatccatgg ctgggctccc tcctgaaaaa aaccgtcacg
      841 atggaagaag ccctcagccg ctgcggcctc accccgtcac cctcaacaaa aaccatccaa
      901 ccatgaacta tcctccccct tctcccatgc cccgtttatc catccccgcg ctcgtgtgga
      961 gcggatgctg cgccatcgca ggaatcactc ttatcgtcta cacctgccag cactacgccc
     1021 aatggagcaa agccctgccc ggttccgggg aattgcgcgc ccaagtggag cacgcccgta
     1081 aaatccgtct gcggcatttt tccctgcagc tggaatggca ggaaaaatcc atcggcatgg
     1141 cgctcacgga acagcaaatg gccaaattga aagaagaaac ttcccaaatc aaacgggaga
     1201 tatgggaaga ggaaagcgcc tggaagaatc tcgcagaaca gattgaccgt caacaaaccg
     1261 cactactcca ggctcaggcg gaaatgcagt ttgccaaaag cgcgctcaga gcggcgggag
     1321 tcagacagaa agatatagaa aaccgcattt cctccctgga gaacgaatta caggaaaaaa
     1381 gccgttgatt tatcgaacgc catgaagaga ttgactattt actccaccct tttccttgcc
     1441 ctgtgccggt ccgcctgggg cctttcctgg tacgccctca ctacgctccg cagcgaaacg
     1501 cgctccatga accgggaact ttccttcttc aatgaacagg aagagcgtta tcagaaaacc
     1561 attccccagc ggatagtgga tgaaaaacgt ttccggcaat tcccttccac catagaaaag
     1621 ttccgggaga tggccgtcca gcaccggcaa atagccggcc gcagacaggc tgtggcggac
     1681 ggcgtctttc agaagtacct ggataaaatt ctggcgtccg cgaaagaaag agaaaaaaga
     1741 atcatcctgc ttcagcgaat cacaaaacag cttgaacaag aagcccagca attaacccac
     1801 aaacgataaa ataccgccat cttatgaaaa ccatcctggc gagcgtctgc ctggccttat
     1861 ccttactact cccggcacgg gcagcgggat atgatattct cctgccctgg ttggcccccc
     1921 aaaggttagg atttacctca ggcaaggcta tccttgattt tgcggaagac aaaaaaagat
     1981 ccgaacttga catgctggtc ttggacaaaa cccggtcgct tcccgccgat atcgcaaacg
     2041 cgttcaacaa gatcaccacc cctgtagcag cttccttcct ggcatacagc aggctgcaca
     2101 acaaaacggt agatgagaac aattttttct ccaagggaat tgcctggtct taccttgtta
     2161 tccgccttca tcaagctgac aaggacaata atcagacagg gtacgttacc ggttggtata
     2221 ctaatgccaa tggatccgat tatcggactt atggcgcttt tgagagcgta tccaggg
//
LOCUS       DAOMSU010000332         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4980_length_2271_cov_3.430054, whole genome shotgun sequence.
ACCESSION   DAOMSU010000332 DAOMSU010000000
VERSION     DAOMSU010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4980_length_2271_cov_3.430054"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="PK077_10270"
     CDS             complement(<1..626)
                     /locus_tag="PK077_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRN24723.1"
                     /translation="MNSSSSSVFPLFDVLVVGAGHAGIEAALAASRLGCSVAVLTHNL
                     DTIGQMSCNPAIGGLAKGHIVREIDAMGGAMGLNTDATAIQFRMLNASKGPSVRAPRA
                     QCDKTAYRTRMKMVLETAPGIRLFQGDVVKLLVNGEQQATGAVTSLGCAIHARSVIIC
                     SGTFMKGLLHVGLQHITGGRMGCASSSLSADLAMLGFQVERLKTGTSPR"
     gene            complement(626..1732)
                     /gene="holA"
                     /locus_tag="PK077_10275"
     CDS             complement(626..1732)
                     /gene="holA"
                     /locus_tag="PK077_10275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRN24724.1"
                     /translation="MGSSTLQQQAIHLVFGTDEGMVREKASGIFAALTEGTNEFSHEI
                     LEAACGDSDEAAQLVRQVMEALRTLPFFPGRKVVWMKNCNFLGDSVTGRSSTTEAALD
                     SFRQLLESGLGPDVLLLISASEFDKRRSFNKFLLQYAASEELNKPDITKAGWEGSLMP
                     LINKETAARGMNFDSAALELFIHRVSESSRQIISEIEKLDLYLGADRRTIMPEDVERM
                     VPLTRTGVIFEISRALENKKSDAAISLIDFQLERGENAITIMRAAFIPTLRNLLAARL
                     LCDAFNLKPTNFKEFTARISSLPSYAAALIPLKKDGTPNAYPLFLAAQNASKFKTERL
                     KQTLKECMKADKALVSSSLDPRLILHRLAICSAS"
     gene            1944..>2271
                     /locus_tag="PK077_10280"
     CDS             1944..>2271
                     /locus_tag="PK077_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24725.1"
                     /translation="MRSTIWTRALLVIMMTVMMTVVSVAYWRWNSRVTEKNVSFRSEV
                     FTLAQQPELKEEDVPILHQLNEEYSRVARLVERVLVSIDTTGVTSVPQPSEDGDFMIE
                     KRLTVHG"
BASE COUNT          547 a          552 c          627 g          545 t
ORIGIN      
        1 cggggagaag ttcctgtttt aagcctttcc acctgaaaac ccagcatggc aagatcggcg
       61 gaaaggctgg aagaagcgca acccattcgg cctcctgtta tgtgttgaag gcccacatga
      121 agcaatccct tcatgaatgt cccggagcag ataatcacgc tgcgggcatg gatggcgcat
      181 cccagagaag taacggctcc cgttgcctgc tgttctccat tgacgagcag tttgaccaca
      241 tctccctgaa acaggcgtat gccgggggcg gtttccagaa ccattttcat tcttgtgcgg
      301 taggctgttt tatcgcactg ggcgcgggga gcgcgcacgc tgggcccctt ggaggcattc
      361 agcatccgga attgaatggc ggtagcatcc gtgttcaatc ccatcgctcc ccccatggca
      421 tctatttccc ggacgatatg ccctttggca agtcccccaa tagccggatt gcaggacatt
      481 tgtccgatag tatccaggtt atgagttagg acggcgacgg aacaccccag acgggatgct
      541 gccagtgctg cttcaatgcc tgcgtgtccg gcgccgacga cgagaacgtc aaacaggggg
      601 aaaacggaag aagaggagga gttcatcagg atgcggagca gatagcaaga cgatgcagaa
      661 tcaggcgggg atcaagagag gaagacacca gggctttatc cgccttcatg cattctttca
      721 aagtttgctt cagccgttcc gtcttgaatt tggaagcatt ctgcgccgcc agaaaaagcg
      781 gatacgcatt gggtgtaccg tcttttttca agggaattaa cgcggccgca taggagggca
      841 gggaagaaat gcgggccgta aattccttga aattggtggg cttcaggttg aatgcgtcac
      901 ataaaagtct ggcagccagc aaatttctta acgtagggat gaatgcggca cgcatgatgg
      961 tgatggcgtt ttcaccgcgt tccagttgaa agtcaatcag agagatggcc gcatcgcttt
     1021 tcttgttttc caaagcccgg gaaatttcaa aaataacgcc tgtacgcgtc agcggaacca
     1081 tgcgttctac atcttccggc attatggtgc gtctgtccgc tcccagataa agatccagtt
     1141 tttcaatttc agaaatgatt tggcgggagg attcgctgac gcggtgaatg aacagttcca
     1201 gggcggcgga gtcgaaattc atgccgcgcg ccgccgtttc cttattaatg aggggcatta
     1261 aactgccttc ccagcctgct ttcgtgatgt ccggcttgtt caattcttcc gatgcggcgt
     1321 attgaagcag gaatttgtta aaggaacggc gtttgtcaaa ttcggaggcg gaaataagca
     1381 gcaggacgtc cggccccagg ccgctttcca gcaattgccg gaaagaatcc agagccgctt
     1441 ccgtggtgga ggaacgcccc gtgacggagt ctcccagaaa attgcagttc ttcatccaga
     1501 ctactttgcg ccccggaaaa aacgggaggg tccgcagagc ttccataacc tggcgtacca
     1561 gctgggccgc ttcatcggaa tcaccgcagg ccgcttccag aatttcatgg gaaaattcgt
     1621 ttgtgccttc cgtcagagcg gcaaagatgc cggaagcctt ttcacgcacc attccttcat
     1681 ccgtaccgaa gacgagatga atggcctgtt gttgtaaagt gctggaaccc ataaaaccgt
     1741 agccgcatga tatcataaag cacgccttgt tgaaaggcgt atttgtcatt tgaaagtttt
     1801 ttcatttgaa gagaaagtac ttaaatagta tcaatggaac cggaatgtca aaaagcggct
     1861 gaagcttgac aggctccgtt ttcaggcatc tccttgtgat caagccgcaa accgtttgat
     1921 cgttattacg gcagggcatt tccatgagat cgaccatatg gacacgggct ttacttgtca
     1981 tcatgatgac ggttatgatg accgtggtct ccgttgccta ttggagatgg aacagtcgtg
     2041 ttacggagaa gaatgtttcc ttccggtcgg aagtgtttac cctggcccag cagcctgaat
     2101 tgaaggagga ggatgttcct attttgcacc agctcaatga ggaatattca cgtgtcgccc
     2161 ggttggtgga acgggtattg gtcagcatag acaccacagg cgtgacaagc gttccgcagc
     2221 cttccgagga tggtgatttc atgattgaaa agaggctgac ggttcatggt t
//
LOCUS       DAOMSU010000333         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4982_length_2271_cov_2.515794, whole genome shotgun sequence.
ACCESSION   DAOMSU010000333 DAOMSU010000000
VERSION     DAOMSU010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4982_length_2271_cov_2.515794"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PK077_10285"
     CDS             complement(<1..410)
                     /locus_tag="PK077_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24726.1"
                     /translation="MHASIFSKLPPEKITALHQSWKNDPLSVDPLWAAWFEGYELGSG
                     EAPGKKENTGTDADTSLYTVPPESAEGRGRISQLIRAYRVMGHQCARFNPLEPPNRPR
                     FPVTPEDMGFQEEDLDQPVNIGTFMDGGTLTLRE"
     gene            complement(474..1511)
                     /gene="cydB"
                     /locus_tag="PK077_10290"
     CDS             complement(474..1511)
                     /gene="cydB"
                     /locus_tag="PK077_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRN24727.1"
                     /translation="MLDNLTLADLQIIWFILVGVLFSGYAILDGFDLGTGALQLFIKG
                     DENRRLTLNAVGPVWDGNEVWLITGGGALFAAFPYVYASVFSGFYLAFMLLLLTLIFR
                     AVSIEFRSKQPMKWWRRGWDATFSISSLLAALLIGVAMGNVTKGIPLDEQGNFTGTFL
                     SLLNPYSVLLGLTTVALFAMHGGIYLLMKTQGSLQEQIRKLLRPCVIIFTVLIILHGA
                     ATLLYVPHVAAALERSPWIYGIAALAVISIASIWLFIHRNRPGWAFVASCSTMGCMMA
                     LFGTAMFPNLLYSMPNPEHSLTLVNGSSTRESLVVMTYIAIMGVPLVLAYSAAIYWVF
                     RGKVQLNEHSY"
     gene            complement(1534..>2271)
                     /locus_tag="PK077_10295"
     CDS             complement(1534..>2271)
                     /locus_tag="PK077_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRN24728.1"
                     /translation="TGGSEKSFKVALVIGLIGVAGMGITGDSSAREVSIHQPAKFAAM
                     EGVMESGAPQDLHLIGWMNPSTHEVTGISIPYLLTLLTHHDLDTSITGMNDIPQDERP
                     PILPVFYSFHLMILIGCALAALFLAALWGWKRGWLFQKRWLLWCFVFSVLGPQIANQA
                     GWAVAELGRQPWIVYGILRTEHAVSPTLTAAEALSSLGMFFIIYALLLALFLYQITHK
                     IHKGPDQETDEDDGAGEGKLQVPFVKD"
BASE COUNT          479 a          576 c          746 g          470 t
ORIGIN      
        1 atttcacgga gcgtgagtgt ccctccatcc atgaaggtgc cgatgttgac gggctggtcc
       61 agatcctctt cttggaatcc catatcctcc ggggtgacgg ggaatcgggg ccggttcggc
      121 ggctccagcg gattgaagcg ggcgcattgg tgtcccatga cccggtaggc ccggatgagc
      181 tggctgatgc gcccgcggcc ttccgcgctt tccggaggaa cggtgtaaag ggacgtgtcc
      241 gcatcggtcc ctgtgttttc ttttttcccg ggggcttctc cgctccccag ttcgtagccc
      301 tcgaaccagg ctgcccagag aggatctacg gacaggggat cgtttttcca tgattgatgg
      361 agcgccgtaa ttttctctgg agggagtttg gagaagatgg aggcatgcat gcggcagagc
      421 ggatggtacg gggcgttttc agttctctga tcgctatttt tgcccgcagg gcgtcaataa
      481 ctgtgttcgt tgagctgaac ttttccacgg aagacccagt aaatggcggc ggaataagcc
      541 agaacgagcg gcacgcccat gatggctatg tatgtcatca cgaccaggct ttcccgcgtg
      601 gatgagccgt tgacgagcgt cagggagtgt tccgggttgg gcatggagta aagcaggttg
      661 gggaacatcg ctgtcccgaa gagagccatc atgcagccca tggtggagca ggaggcgacg
      721 aaggcccagc ccggacggtt cctgtggatg aagagccaga tggacgcgat ggaaatgacc
      781 gccagggcgg cgatgccgta aatccagggg gagcgttcca gggccgccgc tacgtgcgga
      841 acatacagca gggtggcagc cccgtgcagg atgatcagga cagtgaagat gatgacgcag
      901 ggtctgagca gtttcctgat ttgttcctga aggctgccct gggttttcat cagcaggtag
      961 attcccccgt gcatggcgaa cagggcgacc gtcgtcagcc ccagaagaac ggaataagga
     1021 ttcagcaggc tgaggaatgt tccggtgaag tttccctgct catccagagg aatgcctttg
     1081 gtaacgttcc ccatggctac gccgatgagc agggccgcca gaagactgct gatggagaag
     1141 gtggcatccc atcccctgcg ccaccatttc atgggctgct tgcttctgaa ttcaatggac
     1201 acggcgcgga agataagggt gagcagcagg agcatgaagg ccaggtaaaa tccggagaag
     1261 acggaagcgt acacgtacgg aaaggcggcg aagagggcgc ctccgcccgt gatgagccag
     1321 acttcgtttc cgtcccatac ggggcccacg gcatttagtg tgagtcttct gttttcatcg
     1381 cccttgatga aaagctgaag ggcgccggtt cccaggtcaa agccgtccag aatggcgtat
     1441 ccggagaaaa gcacgccgac gaggatgaac cagatgattt gcaggtctgc gagagtgaga
     1501 ttgtccaaca tggttttaaa agaggggtga aggttagtct ttgacaaaag gaacctggag
     1561 tttgccttcc ccggcgccgt cgtcttcgtc agtttcctgg tccggacctt tgtggatttt
     1621 atgggtgatt tgatagagga agagggccag cagcagggca taaatgataa agaacatacc
     1681 cagggaggaa agggcttccg ccgcggtgag ggtgggggat acggcgtgtt ccgttctcag
     1741 gatgccgtac acaatccatg gctggcgccc cagttccgct acggcccagc ccgcttggtt
     1801 ggctatttgc gggcccagta cggagaaaac gaagcaccag agcagccagc gcttctggaa
     1861 gagccagcct cttttccacc cccacagggc cgccagaaac agggccgcca gggcgcagcc
     1921 gatcaggatc atgagatgga aggagtaaaa gacgggcagg atgggagggc gttcgtcctg
     1981 cgggatgtcg ttcatgccgg tgatggacgt gtccaggtca tgatgagtca ggagggtgag
     2041 taagtacggg atggagattc ccgtcacttc atgggtggac gggttcatcc agccgatgag
     2101 atggaggtcc tgtggggcgc cggattccat gacgccttcc atagccgcga atttggcggg
     2161 ctggtggatg gagacttctc gtgcgctgga atctcccgtg atgcccatgc ctgccacgcc
     2221 gatgaggccg atgaccaatg ccaccttgaa gcttttttcc gatccaccgg t
//
LOCUS       DAOMSU010000334         2271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_4983_length_2271_cov_2.406137, whole genome shotgun sequence.
ACCESSION   DAOMSU010000334 DAOMSU010000000
VERSION     DAOMSU010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2271
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4983_length_2271_cov_2.406137"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="PK077_10300"
     CDS             <1..310
                     /locus_tag="PK077_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24729.1"
                     /translation="HREGAMLFPDLPNALAYGRDYAEEIRDSQWRIHYHIPLYASPEP
                     PLKSTEEFILKTHNFLRGRKGPQPHLEVETYTWSVLPDHMKIPLAAQIARELHYIETL
                     "
     gene            315..1274
                     /locus_tag="PK077_10305"
     CDS             315..1274
                     /locus_tag="PK077_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24730.1"
                     /translation="MMPRMHPSAVLSSGFLLLSTGLSSCTFLPMFSSGGQETPRTLGA
                     RMEKHTEVLQKDIGERNASQPKSMEAAAKYIAGNFSDMGYAVTMQPVSGGNVKKGAAI
                     YNILVYKPGLYSNNQSIVVGANYDSSGRRNNGSGTALLLETARGLKDIPTNHNIYFVA
                     YANGAEPAGKSISSGACVHAGTLSGSIGATNILGMINLEPQKLDAGGERKQPGPEASR
                     EAVLFTTTPHGKKFAGQCAAPFAKSWEQAPTTFYREPSALTGNDRHYAVLGIPTVSCR
                     SKYPVTDARTEEYVQALTDMIHQVADNDAPEPAKNTGQGEKAS"
     gene            complement(1293..>2271)
                     /locus_tag="PK077_10310"
     CDS             complement(1293..>2271)
                     /locus_tag="PK077_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN24731.1"
                     /translation="LAGGVIRRAGFKGSYQYACFLCAVSTVLLPVFPGVPAWSGLRLL
                     AGISCALVWVVVESALLRAGTLQTRGILLASYMVVYYLGTVLGQLLLGWFPSDMPLIV
                     TEVCILSVAGMVPLMFARLEPGNGQVSSSSHIEIRTLLRRRSVFLGVVGCVISGVVLG
                     TIYCLMPLFLKHQGMDHSSVGYWMALLIAAAILGQWPMGRLADRYGRAFVMKCQSLLV
                     AAACAGLMLKGGLMAPSLIALGLAGFSLYPVAMAWGCEEASRDELVTMNQLLLLSYSL
                     GTLAGPSLTSFLMQRYSDNWMPMVIALVALSFMPVLMLGGGHGRRKLSR"
BASE COUNT          572 a          753 c          590 g          356 t
ORIGIN      
        1 ccaccgggaa ggcgccatgc ttttcccgga cctgcccaat gccctggcct atggacggga
       61 ctatgcagag gaaatacggg attcccaatg gcgcattcat taccacattc ccctgtacgc
      121 ttcaccggaa ccacccttga aaagcacgga agaattcatc ctgaaaacgc ataatttcct
      181 ccggggccgc aaaggcccgc aaccgcacct ggaggtggaa acctatacct ggagcgtcct
      241 gccggaccac atgaagatcc ccctggcagc ccagattgcc cgtgaactgc attatattga
      301 aaccctgtaa agccatgatg ccccgcatgc atccctccgc cgtcctgtct tccggattcc
      361 tgctgctgtc caccggtctt tcctcctgca catttcttcc catgttttcc tccggggggc
      421 aggaaacgcc gcggaccctg ggcgcacgca tggaaaaaca cacggaagtc ctgcaaaaag
      481 acatcgggga aagaaacgcc agccagccca aatccatgga agccgccgcc aaatacatcg
      541 ccgggaattt ctcggacatg gggtacgccg tcaccatgca gcccgtatcc ggaggaaacg
      601 tgaaaaaagg agcggccatt tacaacatcc tggtttacaa gcccggcctg tattccaaca
      661 atcaaagcat cgtcgtggga gccaattatg attccagcgg acggcgcaac aacggcagcg
      721 gaacggccct gctgctggag acggcaagag gcctgaaaga catccccacc aatcacaata
      781 tctattttgt cgcatacgcg aacggagcgg agcccgcagg gaaatccatc tcttccggag
      841 cctgcgtgca tgccggcacc ctaagcggca gcattggagc aaccaacatc ctgggcatga
      901 tcaatctgga accccagaag ctggatgccg gaggggaacg aaaacagccg ggcccggaag
      961 cctcccggga agccgttctg ttcacgacca ccccgcacgg caagaaattt gcaggccagt
     1021 gcgccgcccc cttcgccaaa tcctgggagc aggcgcccac taccttctac cgggaacctt
     1081 ccgccctcac aggcaatgac cgccattacg cggtcctggg cattcccacg gtctcctgcc
     1141 ggagcaagta tcccgtcacg gatgcccgta cggaagagta tgtgcaggcg ctgacggaca
     1201 tgattcacca ggtagcggac aacgacgcgc ccgaaccggc aaaaaacacc ggccagggcg
     1261 agaaagcatc ctgacgtttc cctctctccc cgttaccggg acagctttct ccttccgtgg
     1321 ccgccgccca gcatcagcac aggcatgaag gaaagggcca ccagcgcaat aaccataggc
     1381 atccaattgt cggaatacct ctgcatcagg aacgaagtca gggaagggcc ggccagcgtg
     1441 cccagggaat aactcaacag cagaagctgg ttcatagtca ccagttcatc ccgggaagct
     1501 tcctcgcatc cccaggccat ggcaacaggg tacagggaaa atccggccag ccccagagca
     1561 atcagggagg gagccatcag cccccccttc agcatcagcc cggcacaggc cgccgccacc
     1621 agcagggact ggcatttcat gacgaaagcg cggccgtacc tgtccgccag ccgccccatg
     1681 ggccactgcc ccagaatggc agcggcaatc agcaaggcca tccagtatcc cacggaagag
     1741 tgatccattc cctggtgctt caggaacagg ggcatcaggc aataaatagt acccaatacc
     1801 acgccggaaa tcacacatcc cacaacaccc agaaagacgc tgcggcgtct cagcagtgtc
     1861 cgaatctcta tatgggagga tgatgaaacc tgtccattgc ccggctccag acgcgcaaac
     1921 atcagcggaa ccatgcctgc cactgataaa atgcagactt ccgtcacaat caggggcata
     1981 tcgctgggga accagcccag aagcaactgc cccagcaccg tgcccagata ataaaccacc
     2041 atataggaag ccagcagaat gccgcgggtt tgcagcgttc cggccctcag cagggcgctt
     2101 tccaccacca cccagaccag ggcgcagctg atccccgcca gcagccggag gccgctccag
     2161 gccggcacgc cgggaaacac aggcagcaga acggtggaca ctgcacataa aaagcatgca
     2221 tattggtaac ttcctttgaa cccggcccgg cgaatcacgc cgccagccag c
//
LOCUS       DAOMSU010000335         2262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5020_length_2262_cov_3.067059, whole genome shotgun sequence.
ACCESSION   DAOMSU010000335 DAOMSU010000000
VERSION     DAOMSU010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5020_length_2262_cov_3.067059"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PK077_10315"
     CDS             complement(<1..564)
                     /locus_tag="PK077_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRN24732.1"
                     /translation="MNHLTRAKSVFEMEIEELRGVLSRLDDNFNKAVDLMSQALDRGN
                     KIVIVGVGKSGNIGAKIVATLNSTGTPTVLLDSLNALHGDLGIVQDGDVCIAMSFSGE
                     TSELLTLLPFIKRFELPIISMTGNTGSSLAKYSDIVLDTGVSREACPLNLAPTSSTTA
                     MLVMGDALAMALVEARHFTARDFAKRHP"
     gene            complement(601..>1405)
                     /locus_tag="PK077_10320"
     CDS             complement(601..>1405)
                     /locus_tag="PK077_10320"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HRN24733.1"
                     /translation="PPLIPVTTMTLTQEETERYARHLSLPELGEQGQYKLKRAHVALT
                     GLGGLGSPAALYLAAAGVGRLTLIDPDEVCLSNLQRQILHATDAAGTAKTASAARRLY
                     ALNPSVRINTVHRRLTPENAVSLLEGCDLVLDASDNYAARFAMADAACTLRIPLVYGA
                     VKGFIGQVAVFAPHQGTACYRCLFPADTPMQEKDTASAAGILGAHAGIIGCIQAMEAL
                     KYLAGIPSPLVGAMLSADTRRMRFTTIPLAPNPACRCRTNEGCGAAMKN"
     assembly_gap    1406..1420
                     /estimated_length=15
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1421..1949)
                     /locus_tag="PK077_10325"
     CDS             complement(<1421..1949)
                     /locus_tag="PK077_10325"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HRN24734.1"
                     /translation="MTTQPQVSSSAVSGNSWWMGILGVIELFLGFIALASPWIVGASF
                     IWVIGIMLMVLAVVRLIQVFTVPSSRGWNLVTAILYGIAGWFLFRDPNISLAITTLII
                     GWGLVIAAVFQGAIWLQTRSLPASGWRLFNVIITLILGLMVIFGWPESTAWFVGTLIA
                     VELIFSGWTLLLYAFS"
     gene            complement(1998..>2262)
                     /locus_tag="PK077_10330"
     CDS             complement(1998..>2262)
                     /locus_tag="PK077_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24735.1"
                     /translation="VISGGLRDGTNSLTLNFSDSPNGKKGAVLVEVYIMPEIPGHLPA
                     RAFSYFVPPQAQPKSGPVLINVTPELLKTMEPKNSSPKAAAGK"
BASE COUNT          471 a          613 c          737 g          426 t
ORIGIN      
        1 ggggtggcgt ttggcgaagt cccgcgcggt gaagtggcgc gcttccacca gagccatggc
       61 gagggcgtct cccatgacga gcatggccgt ggtgctggaa gtgggggcca ggttcagcgg
      121 gcaggcttcc cgggaaacgc ctgtgtccag aacgatatcc gagtatttgg ccagggatga
      181 gcccgtgttg cccgtcatgg aaatgatggg cagttcaaaa cgcttgatga aggggagcag
      241 ggtcagcagc tccgaggttt ctccggagaa agacatggca atgcagacat ccccgtcctg
      301 gacgatgccc agatccccgt gcagggcgtt cagggaatcc agcaaaacgg tgggcgttcc
      361 cgtagagttc agggtagcga cgatcttggc cccgatgttg ccggatttgc ctacgcctac
      421 aatgacgatc ttgttgccgc ggtccagagc ctgggacatg aggtccacag ccttgttgaa
      481 attgtcatcc agccgggaca ggacgccgcg cagctcttca atctccattt caaaaacgga
      541 tttggcgcgt gttaaatgat tcatacctgt tggaaatgat gccagatgcc gccgacgccg
      601 tcaatttttc attgcggctc cgcacccctc gttagtccgg catctgcacg cgggattagg
      661 cgccagaggg atggtggtga agcgcatgcg ccgcgtgtcc gcagagagca tggcgcccac
      721 cagcggagag ggaatgcccg caagatattt cagggcttcc atggcctgga tgcagccgat
      781 gatgcccgca tgcgcaccca gaatgcctgc ggcggaggcg gtgtcttttt cctgcatggg
      841 cgtatctgcc gggaagaggc agcggtaaca ggcggttccc tgatgggggg cgaaaacggc
      901 cacctgcccg ataaagccct tcacggcgcc gtacaccagc ggaatgcgca gggtgcaggc
      961 ggcatccgcc atggcgaagc gtgccgcgta attgtctgaa gcgtccagaa ccaggtcgca
     1021 cccttccagc agggaaacgg cattttccgg cgtaaggcgc ctgtggaccg tgttgatgcg
     1081 tacggagggg ttcagggcgt acaggcgtct ggcggcactg gcggttttag ccgttcccgc
     1141 cgcatccgtg gcatgcagga tttgccgctg gaggttggaa agacacacct cgtccgggtc
     1201 aatcagggtc aggcgcccca cgcctgcggc agcaagataa agggccgccg gggagccaag
     1261 ccccccgagg cccgtcaggg cgacatgcgc ccgtttgagt ttgtattggc cctgttctcc
     1321 cagttccgga agggaaagat gcctggcgta ccgttcggtt tcttcctggg tgagggtcat
     1381 tgtcgtcact gggattaacg gcgggnnnnn nnnnnnnnnn cgctgaatgc atacaggagc
     1441 agcgtccacc cggagaagat cagttccact gcaatcagag tacctacgaa ccaggccgtg
     1501 gattcaggcc agccgaagat gaccatgagg cccaggatca gcgtgatgat cacgttgaac
     1561 agccgccacc cgctggcagg cagggaacgg gtttgaagcc agatggctcc ctgaaatacg
     1621 gcggcaataa ccaggcccca cccgatgatg agcgtggtga tagccaggga aatattgggg
     1681 tcccggaaca gaaaccagcc ggcgatgccg tacaggatgg ccgtcaccag gttccaccca
     1741 cgggaggaag ggacggtgaa gacctggatc agccggacga cggccagaac catcagcatg
     1801 atgccgatta cccaaatgaa gctggctccg acgatccagg gagaggccag ggcgatgaag
     1861 ccaagaaata gctcgatgac gccaaggatt cccatccacc atgagttgcc ggataccgcg
     1921 gagctgctga cttgaggttg agttgtcata tggcggtatg accgggcccg caacggaaac
     1981 gttgaagaat gacgatttta tttgccggcg gctgccttcg gggaagaatt cttaggctcc
     2041 atggtcttca gcaattccgg agtgacgtta atcagcacag ggccgctttt gggttgcgcc
     2101 tgggggggga cgaagtagga aaaagccctg gcgggcaggt ggcccgggat ttccggcatg
     2161 atgtacactt ccacgagtac ggcgcctttt ttgccgttcg ggctgtcgga aaagttcagt
     2221 gtgagtgagt tggtcccgtc cctcagtcct ccggaaatga ct
//
LOCUS       DAOMSU010000336         2255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5047_length_2255_cov_2.091818, whole genome shotgun sequence.
ACCESSION   DAOMSU010000336 DAOMSU010000000
VERSION     DAOMSU010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5047_length_2255_cov_2.091818"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="PK077_10335"
     CDS             <1..429
                     /locus_tag="PK077_10335"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRN24736.1"
                     /translation="VPDEGVMAALQAAALRNVDVRILRPERADHILVKLSSFTFLRDL
                     DTYGIQLWAYQKGFLHQKVVLMDDDIATVGTANLDNRSLALNFEITAVIHSPSACAEV
                     KAMLERDFSSSKRESLADYNNKSLGFKMLCNLARLTAPVQ"
     gene            536..1504
                     /locus_tag="PK077_10340"
     CDS             536..1504
                     /locus_tag="PK077_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24737.1"
                     /translation="MNPVTFNCTVLRDGHQSLAATRMKTADMLPIAPILDSMGFSALE
                     TWGGATIDAGLRFLKEWPFDRLDALKKAAPKTPHMMLLRGQNIVGYTNYADDVVEAFV
                     AMSAKHGMNIFRIFDCVNDPRNMETSIRAAKKAGAQAHGTICYTTSPVHTTQTFVDMG
                     RELADMGADAIVIKDMAGLIPPYVTQELVSALKKDLNIPVWIHTHDTAGLGASTYLSA
                     IDAGVDACDVSISPFANGTGQPDCLRMLALPASRITTRTSSLKSPKCSNPSMKVWANS
                     PPTATKWWTLTRSATRCPAACFPTSAPSSRNRAWKTNLRKYSRKSR"
     gene            1423..2043
                     /locus_tag="PK077_10345"
     CDS             1423..2043
                     /locus_tag="PK077_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24738.1"
                     /translation="MLSNFRTQLKEQGMEDKFEEVFAEIPVVRKALGWIPLVTPTSQI
                     VGVQAMLNVKFGRWKNITPQAADIALGYYGRTSAPVDPEVQKLCAEKMGKDPITCRPA
                     DLIKPGMEDLRKKLAEKGLPTDDEHCVIYAMFPQQVEDFYKKPEPKEEAPVQVNTAPA
                     AAPAAAPSMTVIDNGITARVSGPSGSRDLTIIINGQSHSVKVERID"
BASE COUNT          502 a          737 c          597 g          419 t
ORIGIN      
        1 gtcccggacg agggagtcat ggccgccctg caggcggcgg ccctgcggaa cgtggatgtg
       61 cgcatcctgc gtccggagcg ggcggaccat attctggtga aactgtcctc ctttactttc
      121 cttcgggacc tggacacata cggcatccag ctctgggcct accaaaaggg gttcctgcat
      181 cagaaagtgg ttctgatgga cgatgacata gccaccgtgg gaaccgccaa tctggacaac
      241 cgttccctgg ccctgaactt tgaaataacc gccgttatcc attccccctc cgcctgcgcg
      301 gaggtaaaag ccatgctgga gcgggatttt tcctcctcca agagggaatc cctggcggat
      361 tacaacaaca aatccctggg cttcaaaatg ctctgcaacc tggcgcgact gacggccccc
      421 gtccagtagt tcttccggcg aatccgctgt tgtatgcttg acctgcaagg gaggctgtga
      481 gaaaatcacc ctccagaagc cacctcttca atttttaaca acagttaact aaataatgaa
      541 tcctgttaca ttcaactgca ccgttctgcg cgacgggcat caatccctgg cggcaacacg
      601 catgaagacg gcagacatgc tgcccatcgc ccccattctg gactccatgg gcttcagcgc
      661 cctggaaacc tggggcggcg ccaccatcga cgccgggctg cgcttcttga aagaatggcc
      721 tttcgaccgg ctggacgccc tgaaaaaagc ggcccccaag acgccgcaca tgatgctgct
      781 gcgcggccag aatatcgtgg gctacaccaa ctatgcggac gacgtggtgg aagcctttgt
      841 cgccatgagc gccaagcacg gcatgaacat cttccgcatt ttcgactgcg tgaacgaccc
      901 gcgcaacatg gaaacctcca tccgcgccgc caagaaagcc ggagcgcagg cccacggcac
      961 catctgctac acgacttctc ccgtgcacac cacgcagact tttgtggaca tggggcggga
     1021 actcgcggat atgggagcgg acgccatcgt catcaaggat atggccggcc tcattcctcc
     1081 gtatgtcacc caggaactgg tcagcgccct gaagaaagac ctgaacatcc ccgtctggat
     1141 ccacacgcat gacaccgccg gcctcggcgc atccacttac ctcagcgcca ttgatgccgg
     1201 agtggacgcg tgcgatgtct ccatctcccc cttcgccaac ggcacgggcc agccggactg
     1261 cctgcgcatg ctcgccctgc ccgcaagccg gattacgacg cggacaagct cattgaaatc
     1321 tccgaaatgc tcaaacccgt ctatgaaagt ctgggcaaat tcgcctccca ccgcaacgaa
     1381 gtggtggact ctgacacgct ccgctaccag gtgcccggcg gcatgctttc caacttccgc
     1441 acccagctca aggaacaggg catggaagac aaatttgagg aagtattcgc ggaaatcccg
     1501 gtagtccgca aggccctggg ctggattccg ctggtgaccc ccacctccca aatcgtaggc
     1561 gtgcaggcga tgctgaacgt caagttcggc cgctggaaga acatcacccc ccaggcggcg
     1621 gacattgccc tgggctacta cggccgcacc tcggctcccg tggacccgga agtgcagaaa
     1681 ctctgcgcgg aaaagatggg caaggacccg atcacctgcc gtccggcgga cctgatcaag
     1741 cccggcatgg aagacctgcg caagaagctt gcggaaaagg ggctccccac ggacgacgaa
     1801 cactgcgtca tctacgccat gttcccgcag caggtggaag acttctacaa aaagccggaa
     1861 cctaaggaag aagcccccgt gcaggtgaac accgctcccg ccgcggctcc ggcggccgcg
     1921 ccgtccatga ccgtcattga caacggaatc accgcccggg tgagcggccc ctccggttcc
     1981 cgtgacctga ccatcatcat caacggccag tcccactccg tgaaagtgga acgcatcgac
     2041 taaaatctcc gaagcatctg ctcaaaccgt ttgcgggcag cccctccata aagggactgc
     2101 ccgctttgca tgaacgctcc tgcttcttat tcaaccactc tgttttccca cagatagggg
     2161 gacttacgcc gccaaccaga aagcacgggc atcctgcctt ctgatttcat ccatattcag
     2221 atatctggtt ttcaataaat gggcgttttg gtgcc
//
LOCUS       DAOMSU010000337         2250 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5061_length_2250_cov_2.466059, whole genome shotgun sequence.
ACCESSION   DAOMSU010000337 DAOMSU010000000
VERSION     DAOMSU010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5061_length_2250_cov_2.466059"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1772
                     /gene="ccsA"
                     /locus_tag="PK077_10350"
     CDS             171..1772
                     /gene="ccsA"
                     /locus_tag="PK077_10350"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRN24739.1"
                     /translation="MNLGGSRTGPVKWLLALNACPAKMAGEPFLRSDNRDMVEFLGGR
                     GRYFSYADAVEKAGLLRGSLRGNSHQAHAAEAVLGALAVYEQAGVALALRLEGTSGCL
                     GTLDAWHEAMVEASAELEAAEREKRHPSLDRLYRANRYFQFLQALVQVEEADPNVALR
                     VIPHEGSYLTPAAALLEKNLSPAGRTALKAYAAACDAYAAGDESTAAETLRQLSGELE
                     SILPMDSFKLHFEHCINALEPFFGGFLLYGMSLVCFVVSLFLKRRKALLHFATVFLCM
                     AVLWHLFGIAARMYIQSRPPVTNLYSSVVFAGVVASLFGGTLYILRRQLPVAAVACAS
                     GLLSLLVAMNLPYSGDTMGMMRAVLNSNFWLTMHVMTIMIGYGSVFFAGFLASYHLVA
                     RFFGFGQGEKGLKAGSCGVYRILLVALFFCFLGTMLGGIWADMSWGRFWGWDPKENGA
                     LMTLLWCSCTLHARLLHVCSCQGFLVLASLGNIVAAWGWFGVNLMGIGLHSYGFVKGG
                     WFWFFLFVGLQVLAAASALILQRSR"
BASE COUNT          465 a          559 c          606 g          620 t
ORIGIN      
        1 ttgtgattgc attattgcct ctgtttcaga ggttggggaa ttctttttcc tcatttgacg
       61 gcataggggc gccctcaccc ggagcgctgc catgcctgaa ggcaggacga gtgatgccgg
      121 tttcaagcgc agcggcggat tcccttaaat tggcaagcgg ccgcacgtcg gtgaatcttg
      181 gaggctctcg gaccgggccg gtgaagtggt tgctggcatt aaacgcctgc ccggcaaaaa
      241 tggccggcga accttttctg cgttcggata acagggatat ggtcgaattc cttggcggac
      301 gaggccgtta tttttcctat gccgatgcag ttgagaaggc tggcctgctg cgcggctctc
      361 tgcggggcaa ttctcatcag gcccatgccg cggaagccgt actgggcgca ctcgccgttt
      421 atgagcaggc cggcgttgcg ctcgctctcc gcctggaggg gacatcgggc tgtttgggga
      481 cgcttgatgc atggcatgaa gctatggttg aagcttcagc agagcttgaa gccgctgaac
      541 gcgaaaaacg ccatccctcg ctagaccggc tctaccgtgc caatcgctat tttcaattcc
      601 tccaggctct tgtgcaggta gaagaagcgg acccgaatgt ggccctgcga gtaatcccac
      661 atgagggaag ctacctgact ccggctgctg ctctgctcga aaaaaatctc agtcccgccg
      721 ggcggaccgc cctgaaagcc tatgccgcag cctgcgatgc ctatgctgcc ggagatgaat
      781 ctactgctgc agagactctg cgtcaacttt ccggggaatt ggagtcaata ctgcccatgg
      841 attctttcaa actgcatttc gagcattgca tcaatgctct cgaacccttc tttggaggct
      901 tccttctcta cggcatgtcg ttggtttgtt tcgtagtctc tctgtttctc aagcggcgga
      961 aggcattgct gcattttgcg acggtcttcc tgtgtatggc ggtattatgg catttattcg
     1021 gcattgcggc ccgcatgtac attcaatcgc gccccccggt taccaatttg tattcctcag
     1081 tcgtctttgc gggcgtggta gcctcattat tcggcgggac gctttatatt ctccgccgtc
     1141 agcttcctgt tgccgcggtc gcctgcgctt ccggccttct ctcactgctg gtagccatga
     1201 atttgcccta cagcggcgat accatgggca tgatgcgtgc cgttctcaat tcgaatttct
     1261 ggctcaccat gcacgtaatg accatcatga taggctacgg aagtgttttt tttgccgggt
     1321 ttttagcctc atatcatctg gtggcccgtt tcttcggctt cggacagggt gaaaaggggc
     1381 tgaaagccgg gtcctgcgga gtctatcgca tccttctcgt ggcgctcttc ttttgttttc
     1441 tggggacaat gctcggcggc atctgggctg acatgagctg gggacggttt tggggatggg
     1501 accctaaaga aaacggtgcc ttaatgacac tcctttggtg ctcctgcacc ctccatgccc
     1561 gccttttaca cgtctgttcc tgtcaaggct tcctcgttct tgcctcgctc ggaaatatcg
     1621 tagcagcctg ggggtggttc ggtgtcaacc tcatggggat tggcctgcac tcctacggtt
     1681 ttgtgaaagg tggctggttc tggtttttcc tttttgtggg ccttcaggtc cttgcggcag
     1741 cttcagccct gattctccaa aggagccgct gatgatttta cgaaccatcg ggcagggaag
     1801 gggaggcgtt cgcgacatac ggcattgatg gctgtcccgc agctgtccgg gacaacatta
     1861 atactttgct tttttattta gcataacata aaaataaatg atttgtgacg ctttgatgta
     1921 aaagcgggag ttgaattttt tctgtggcgc cgcaggctga tataatgaat tttattttgc
     1981 gttatacgga attgcgatgg aagaatcatc cagaaacgcg caatatgtgg taaatccgga
     2041 taaacggaaa ataaaaccat gaatgtatgg aataaattat atgaagaaac tttgcgaatt
     2101 taaaatgaca gatgtatctc gccatttatc aatgcagaaa cgtatttgat acatcatctg
     2161 ctctcggaat gcggaatatg tacagaaata aatgatctcg cccataagca ataaatgcaa
     2221 atatggggga aatacttaaa ttaatccatt
//
LOCUS       DAOMSU010000338         2234 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5115_length_2234_cov_1.983020, whole genome shotgun sequence.
ACCESSION   DAOMSU010000338 DAOMSU010000000
VERSION     DAOMSU010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5115_length_2234_cov_1.983020"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK077_10355"
     CDS             <1..183
                     /locus_tag="PK077_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24740.1"
                     /translation="SYVEPFIPLVIFSLYEGILYFNFVSGLQGGMFSFGKYTDNFFEN
                     LYFPFSGDHGNQWIRK"
     gene            complement(358..>553)
                     /locus_tag="PK077_10360"
     CDS             complement(358..>553)
                     /locus_tag="PK077_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRN24741.1"
                     /translation="ENADAYCRMVLMAGLHKAPLNQVGPEGVADFLAIRKSIGYAVPD
                     NQPLYNTETYAGYKLGKSGK"
     assembly_gap    554..576
                     /estimated_length=23
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<577..1167)
                     /locus_tag="PK077_10365"
     CDS             complement(<577..1167)
                     /locus_tag="PK077_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRN24742.1"
                     /translation="MSEDWSISPPLPDKVFPWEKYNPVTREEVNEFFHSPEILVIKER
                     MCDIGRRLWNREYVDGNGGNISVRVAQNLLLCSPTLCSKGFMTVEDICMVDMDARQKA
                     GIRPSTSEVKTHIAMMKSVGVNACIHAHPPHCNAFLFAGQVPPSGINPEADIFLGHIP
                     LAPYGTPGSLETARAVAEAARQSTVVFMENHGVITGA"
     gene            complement(1181..>2234)
                     /locus_tag="PK077_10370"
     CDS             complement(1181..>2234)
                     /locus_tag="PK077_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HRN24743.1"
                     /translation="NRISGVGAFAFNTIYKLVWLRENHPELLEKAHAWLFISNLLAYK
                     LTGIMATDRTMAGTSQLTDLETGDFSEFILNSRGLFPPIVDAGETIGLLTPEAADAMN
                     LPALAGIPVISAGHDTQFAIFGSGADKNQPVLSSGTWEILMARSAQARLAREDYEDGA
                     TAEFDAESGLLNPGLQWLGSGIIEWVKAACFHGESYDTMDAEAAAVPPGCDGVTMVPD
                     FLASGDRKGSINGLVLGRTRGHIYRAAMEALTWRLKSRLRRLESVGGFKSEFLILVGG
                     GARNRVWTQMRADILRIPVKVSEVSESTVLGASMFAFAGAGVYSTPEQARAAFGITYR
                     TYMPGSQEAAYEKFNQ"
BASE COUNT          443 a          579 c          685 g          504 t
ORIGIN      
        1 agttatgtgg aaccgtttat tcccttagtt attttttctc tctatgaggg gatattatat
       61 ttcaattttg tttccgggct gcaggggggt atgttctcat ttgggaaata tacggataat
      121 ttttttgaaa atctgtattt tcctttttcc ggggatcatg ggaatcaatg gattcggaaa
      181 taaggaggcc attctgtaaa gcagatagca ggagagacgg gcaaaacagt ccatgggaaa
      241 ggaagggagc ccgatttggt ccgtgtgtga accctatgaa agaaaggagc agagaggaag
      301 ccgcaaaaaa gggcgcccgg catgcgacgg ctccccaatg aagaacggtg aattccgtta
      361 ttttccggat tttcccagtt tgtacccggc gtaggtttcc gtgttgtaca ggggctggtt
      421 gtccggcacg gcgtaaccga tggatttgcg aatggcaagg aagtccgcca cgccttccgg
      481 ccccacctgg ttcagaggtg ctttgtgcag gccggccatc agcaccatgc ggcagtaggc
      541 atccgcattt tccnnnnnnn nnnnnnnnnn nnnnnntgct ccggtaatga ctccgtggtt
      601 ttccatgaaa acgacggtgg attgtctggc tgcttccgcc acggcccggg ccgtttccag
      661 agagccgggc gttccgtaag gggccagcgg gatgtgcccc aggaagatat ccgcttccgg
      721 attgatgccg gaggggggaa cctgcccggc aaacaggaag gcattgcagt gcgggggatg
      781 ggcatggatg caggcattga cgcccacgga tttcatcatg gcaatgtgcg ttttcacctc
      841 gctggtggag gggcggatgc ccgctttctg gcgcgcgtcc atgtccacca tgcagatgtc
      901 ttccacagtc atgaagcctt tggaacacag ggtgggggag cagagcagca ggttctgagc
      961 cacgcgcacg gagatgttgc ccccgttgcc gtccacatat tcccggttcc acaggcggcg
     1021 ccctatgtcg cacatgcgtt ccttgatgac cagaatttcc ggggaatgga agaattcgtt
     1081 cacttcctcc cgcgtgacgg ggttgtattt ttcccatggg aacactttgt ccggcagggg
     1141 aggggagatt gaccagtctt ctgacatatt tagtagcgtg ttattggttg aacttttcgt
     1201 aggcggcttc ctgggaaccc ggcatatatg tccggtaggt gatgccgaag gcggcccggg
     1261 cctgctccgg cgtagagtac accccggcgc ccgcaaaggc aaacatggat gcgcccaata
     1321 ccgtactttc tgacacttca gagactttga cgggaatacg gagaatgtcc gccctcatct
     1381 gggtccaaac tctgtttctg gcgcctccgc ccactaggat gaggaattcg cttttgaagc
     1441 cgcccacgga ttccaaacgg cgcaggcggg atttcagccg ccaggtgaga gcctccatgg
     1501 cggcgcggta gatatggccg cgtgttcttc ccagcaccag tccgttgatg gagcctttcc
     1561 ggtctccgga ggccaggaag tccggaacca ttgtcacgcc gtcgcagccg ggtgggacgg
     1621 cggccgcttc cgcatccatg gtgtcatagg attccccgtg aaagcaggct gctttcaccc
     1681 attcgataat gccggagccc agccattgga gcccggggtt aagaaggccg gattccgcgt
     1741 cgaattccgc cgtggcgccg tcctcataat cttcccgggc cagcctggcc tgtgcggagc
     1801 gcgccatcag gatttcccat gttccggagg aaaggacggg ctggttcttg tctgctccgg
     1861 aaccgaaaat ggcgaactgg gtgtcgtgcc cggcggagat aacgggaatg cccgccaggg
     1921 ccgggagatt catcgcgtct gccgcttccg gcgtaaggag gccaatggtt tcccctgcat
     1981 ccacgatggg ggggaagagg ccgcggctgt tcaaaatgaa ttcggaaaaa tctcccgttt
     2041 ccagatccgt gagctgggag gtccctgcca tggtccggtc tgtcgccatg atgccggtga
     2101 gcttgtaggc cagcaggttg gagatgaaga gccacgcatg ggctttttcc agaagttccg
     2161 ggtggttttc cctgagccag acgagtttgt aaatggtgtt gaaagcaaag gcccccacgc
     2221 cggagatacg gtta
//
LOCUS       DAOMSU010000339         2207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5208_length_2207_cov_2.611059, whole genome shotgun sequence.
ACCESSION   DAOMSU010000339 DAOMSU010000000
VERSION     DAOMSU010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5208_length_2207_cov_2.611059"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(146..787)
                     /gene="bioD"
                     /locus_tag="PK077_10375"
     CDS             complement(146..787)
                     /gene="bioD"
                     /locus_tag="PK077_10375"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HRN24744.1"
                     /translation="MRNFIVTGTDTEVGKTYVSCLIVKSLREAGINAAGFKPVACGDR
                     QDARLLREAGPKGLTLNELNPVFLRNATCPYVAARLENTQVDEEVIRNAYDALSAAHE
                     CVLVEGVGGWEVPIGPGRNFSDMAADFKLPVLLVIGNKLGAINHALLTLNAIKRRGLE
                     CLGIVFNNVKEEWDTACVTNRSMVEEFSDAPILGELIHGQDYMDMEPLLKRLL"
     gene            complement(800..952)
                     /locus_tag="PK077_10380"
     CDS             complement(800..952)
                     /locus_tag="PK077_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24745.1"
                     /translation="MKKLLLLNWPAKLFCLVLAVIIWSFINHWVTTNDGVSTRGQLEE
                     IRRSHP"
     gene            complement(930..1721)
                     /gene="cdaA"
                     /locus_tag="PK077_10385"
     CDS             complement(930..1721)
                     /gene="cdaA"
                     /locus_tag="PK077_10385"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRN24746.1"
                     /translation="MWDWIVIKDALRAVVEIFILWVFLYQIYRAFHATRGARIMVGLF
                     ACFLALVVLAFFFQLNVISWILTRIFAPGLALALVVIFQPELRVGLAKLGSHPFFSSF
                     AKLQRVDFLDNFCKAVSKLSNQRFGALFAFERSISMKPIEDSGVKLDAIFSPELALTI
                     FHTKTALHDGGVVISGDRISAAACVFPVSQKEMSDRTLGLRHRAGVGMSEESDCVVVV
                     VSEETGAIALAVGGKLERNLTPEQLKGRLEELLNISSSNEKTAIA"
     gene            2026..>2207
                     /locus_tag="PK077_10390"
     CDS             2026..>2207
                     /locus_tag="PK077_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24747.1"
                     /translation="MKGAGKIWLTWVKLQDRDPAEVTQIFEQIKGKLDIKLGRGWQKY
                     DPVIKKGRPQSYKKLF"
BASE COUNT          608 a          658 c          508 g          433 t
ORIGIN      
        1 tccacgcctg ggaaaccgta gcgttctccc catccgcccc aacacgacat gattgttcct
       61 tcacatcgct gtttcactcc gcaaacccgg caataaggga caggcgcctt caccgctatc
      121 cctctcattc gctacccccg ccctgtcaga gcaaacgttt cagcaggggt tccatatcca
      181 tgtaatcctg cccatgaatc agttcgccga gaatgggcgc atcggaaaac tcctctacca
      241 tgctcctgtt cgtcacgcag gcagtatccc attcctcttt cacattatta aacacaatgc
      301 ccaggcattc caagccgcgt ctcttgatcg cattcagcgt cagcagggca tggttgatgg
      361 cccccagctt gttgcctatc accagaagaa caggcagctt gaaatccgca gccatgtcgc
      421 tgaaattccg tccggggcca atggggactt cccagccccc caccccttcc accagcacgc
      481 attcatgtgc ggcggacagc gcgtcataag cgttccggat aacctcttca tccacctgcg
      541 tattctccag ccgtgcggct acatagggac atgtggcatt cctcaaaaaa acgggattca
      601 gttcattcag cgtcagcccc tttggcccgg cttcacgcag aaggcgggca tcctgcctgt
      661 ctccgcaggc cacgggcttg aacccggcgg cattaatgcc cgcctctctt aaagatttga
      721 cgatcaggca gctcacataa gttttcccga cttccgtatc ggtaccggta acaatgaaat
      781 tcctcataac ggtaaatatt cagggatggg atcttctaat ctcctcaagc tgcccgcggg
      841 tggacacgcc gtcattcgta gtcacccagt gattgatgaa agaccagata atcacggcca
      901 gcaccagaca gaacagcttg gccggccagt taagcaatag cagttttttc attggaagaa
      961 gaaatattca gcagttcctc aagacgcccc ttcaactgtt ccggagtcag gttgcgttcc
     1021 agtttcccgc ctacggccag ggcgatagcc cccgtttcct cggacaccac caccaccacg
     1081 cagtcgctct cctcggacat gcccacgccc gcgcggtggc gcagccccag ggtgcggtcg
     1141 ctcatttcct tctgggaaac cgggaacacg caggcagcag cggaaatgcg gtcgccggaa
     1201 attaccacgc ctccgtcgtg aagagccgtc ttggtatgga aaatcgtcag ggccagctcc
     1261 ggagaaaaaa tggcgtccag cttcacgccg gaatcctcaa taggcttcat gctgatgctc
     1321 cgctcaaaag cgaacagggc gccgaaccgc tgattggaaa gcttgcttac cgccttgcag
     1381 aaattatcca ggaaatctac ccgctgaagc ttggcgaagg aagagaaaaa cgggtggctt
     1441 cccagctttg ccaaccccac gcgcagctcc ggctggaaaa tcaccaccaa agccaacgcc
     1501 agaccggggg cgaaaatgcg cgtcagtatc catgaaataa cattcagctg aaagaaaaag
     1561 gccagcacca ccagggccag aaagcaggcg aacagcccca ccataatgcg cgcgccgcgc
     1621 gttgcgtgga aagcgcggta aatctggtac aggaacaccc acaggatgaa aatttcaaca
     1681 accgcccgga gcgcatcctt gatcacaatc cagtcccaca tgcccaacaa actacacaac
     1741 aacccacccg aatgcaatac cgatcctctc gcgttgccgg aaggcgccgg gaatttaccg
     1801 ttcatctcaa ttccccaaat ctgaaaacat ccctgtacgt tccggagaca gatagaagct
     1861 ctcaaaaaaa agaacggctc tttgggaaac aaataataac aataagcaaa aatcggatta
     1921 aattaacgat gtaaatccgc ataaagcaag acttcaaaaa acagggattt ttcccggctc
     1981 tgcgcatgag cttgcgacgg atacgctgaa aaaccaaaca cgaaaatgaa aggagcagga
     2041 aaaatctggc tcacctgggt gaaacttcaa gacagggatc ctgctgaagt tacgcaaata
     2101 tttgaacaaa ttaaagggaa actggatata aaattaggaa gaggctggca aaaatatgac
     2161 ccggtaataa aaaaaggaag gccccaatcc tataaaaaac tctttca
//
LOCUS       DAOMSU010000340         2200 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5234_length_2200_cov_2.560373, whole genome shotgun sequence.
ACCESSION   DAOMSU010000340 DAOMSU010000000
VERSION     DAOMSU010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5234_length_2200_cov_2.560373"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /gene="coaD"
                     /locus_tag="PK077_10395"
     CDS             <1..477
                     /gene="coaD"
                     /locus_tag="PK077_10395"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HRN24748.1"
                     /translation="DPLTNGHLWMIRQGARMFDELIVAMGDNPDKRYTFSHEERMDML
                     RVALSDMPDVRIAEFHNRFLVDFANEHGATFMLRGIRSTQDYEYERVMRHINADMAPK
                     VCTVFLMPPRDTAELSSSMVKGLIGPEGWESQVSRYVPHNVFAMLKEKYREVFPRS"
     gene            complement(502..864)
                     /locus_tag="PK077_10400"
     CDS             complement(502..864)
                     /locus_tag="PK077_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HRN24749.1"
                     /translation="MVRPEKLAALRERMEDLGIREQDLEESFVRGSGRGGQKVNKTNN
                     CVYLRHTPTGIAVKCHADRSRELNRFLARRELCDAVEQMQTGRCAARARVIQRMRKQK
                     DRRRRRRSAPAPSPPDSE"
     gene            complement(851..1405)
                     /locus_tag="PK077_10405"
     CDS             complement(851..1405)
                     /locus_tag="PK077_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24750.1"
                     /translation="MPEPERHTPVSAPRPVQAGLGVEDIRHIPVQKRELRFTRNRAGA
                     ILTAAGVLLAATAAFLQLTGYDTITPYLPAPLWAMQAAALVPAVLCLTAGCRCLKHAA
                     VIVTPVGVEILPFLRARRAMQWFFWQQIRSADREGGRLNLRLADGSTAAVSLRPMTAA
                     SRDMLAHAIRQRVNTLQSSGYGQA"
     gene            complement(1392..>2200)
                     /locus_tag="PK077_10410"
     CDS             complement(1392..>2200)
                     /locus_tag="PK077_10410"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRN24751.1"
                     /translation="GSGWLDRLLADGVKYAFVSNSDNLGAQLDMNFLRWFAESGAPFV
                     MEVTRRTEADRKGGHLAVRKSDGQLILREVAQCPDEDIPEFQNISRHRYFNTNTLWIR
                     LDALKEILDANGGVLPLPMIRNKKTLNPRDPESAPVYQLETAMGAGIECFPGARAVNV
                     PRSRFFPVKTTSDLFLLRSDAIVVDEHGNVALAPERQGNTPVVDLDSKLYKLVDSLDG
                     LGLPSLTGMDKLTLRGRFHFQDGAVLRGTLLMENDSDETKEILPGVYAGA"
BASE COUNT          383 a          631 c          730 g          456 t
ORIGIN      
        1 gaccctctga ccaatggtca tttatggatg atcaggcagg gcgcgaggat gtttgatgag
       61 ctgattgtgg ccatgggcga caatccggac aagcgttata cgttttccca tgaggaacgc
      121 atggatatgc tgcgggtggc tctttccgat atgccggatg tgcgcattgc cgagttccac
      181 aaccgctttc tggtggattt cgccaatgaa cacggggcta cgttcatgct gcgcggcatc
      241 cgttccacgc aggattacga gtatgaacgc gtgatgcgcc atatcaatgc ggatatggcc
      301 cccaaggtct gtacggtgtt cctgatgcct ccccgggata cggcggagtt gtcctccagc
      361 atggtgaaag ggttgattgg gccggaaggc tgggagagcc aggtgagccg ctatgtcccc
      421 cacaatgtat tcgccatgtt gaaggagaag taccgtgagg tttttccgcg ttcctagcgg
      481 agattccgga tttccgttcc gttattcgct gtccggtgga gagggggcag gggcggaacg
      541 cctgcgcctc cttctgtctt tctgctttct catgcgctgg atgacgcggg ctcttgcggc
      601 gcatcggccc gtttgcattt gttccacagc atcgcacagt tccctgcgcg ccagaaagcg
      661 gttgagttcc ctggatctgt ccgcatggca tttgacggcg atgcccgtgg gcgtgtgcct
      721 gaggtaaacg cagttgttgg ttttatttac tttctggccg cctcgcccgc ttccgcggac
      781 aaagctttcc tccaggtcct gttcacggat gcccaggtcc tccatccgtt cccggagagc
      841 cgccagtttt tcaggcctga ccatagccgg aggattgcag ggtgttgacg cgctgccgga
      901 tagcatgggc cagcatgtcc cggctggcgg ccgtcatggg gcggaggctg acggcagcgg
      961 ttgagccgtc agccaggcgg aggtttagcc gtccgccttc ccggtccgca gagcgaatct
     1021 gctgccagaa gaaccattgc atggcgcgcc gcgcccgcag aaagggcagg atttccactc
     1081 ccaccggggt gacgatgacg gccgcgtgtt tcaggcagcg gcaccccgca gtcaggcaga
     1141 ggacggcggg aacaagcgcc gcggcctgca tggcccacag gggggcgggg aggtacgggg
     1201 taattgtatc atatcccgtg agctggagga aagcggcggt ggctgccaga aggacccccg
     1261 ccgccgtcag gatggcgcct gcccggttcc gggtgaagcg cagttccctc ttttggacgg
     1321 gaatgtggcg gatgtcttcc acgcccagcc cggcctggac cggacggggc gcggaaacgg
     1381 gagtatggcg ttcaggctcc ggcatagacg cccgggagga tttccttcgt ttcgtcggaa
     1441 tcgttttcca tcagcagggt gccccggaga acggctccgt cctggaagtg gaagcggccg
     1501 cgcagggtca gcttgtccat gcctgtcagg gagggcaggc ccagcccgtc cagggaatcc
     1561 accagcttgt acaatttgga atccaggtcc accaccgggg tgttgccctg ccgttccggc
     1621 gccagggcca cgttgccgtg ttcatccaca acgatggcgt ccgaacggag caggaacagg
     1681 tcggaagtgg tcttgacggg gaagaaacgg gaacgcggga cattgacggc cctggctccc
     1741 gggaaacatt cgatgcccgc gcccatggcc gtttccagct ggtacaccgg ggcggattcc
     1801 ggatcgcggg ggttgagcgt ttttttattg cggatcatgg gcagcgggag cacgccgccg
     1861 ttggcgtcca gaatttcctt gagggcgtcc aggcgtatcc agagcgtatt ggtgttgaag
     1921 tagcggtgcc tggagatatt ctggaattcg ggaatgtcct catccgggca ctgggccact
     1981 tcccgcagaa tcagctggcc gtccgacttc ctgaccgcca ggtggccgcc tttcctgtcc
     2041 gcctccgtgc ggcgggtgac ttccatgacg aagggggcgc cgctttccgc aaaccagcgg
     2101 aggaagttca tgtcaagctg ggcgcccaga ttgtcggaat tggagacaaa ggcgtattta
     2161 acgccatccg ccagcaggcg gtccagccag ccggacccca
//
LOCUS       DAOMSU010000341         2194 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5263_length_2194_cov_2.243572, whole genome shotgun sequence.
ACCESSION   DAOMSU010000341 DAOMSU010000000
VERSION     DAOMSU010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5263_length_2194_cov_2.243572"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="PK077_10415"
     CDS             <1..320
                     /locus_tag="PK077_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24752.1"
                     /translation="ELELLGKDRPSVKSFYVNDRVVMLDESAEIQRVSRDALDRPEIF
                     LARRQPLPWGGSGLSPVDSGYSADDGTVPTPWKDSLFLFLMGAAPLLVTGRVLKRRLE
                     RAG"
     gene            517..1071
                     /locus_tag="PK077_10420"
     CDS             517..1071
                     /locus_tag="PK077_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24753.1"
                     /translation="MFSRLTNRCVLSSLSAAALSLGLFSCGGSGSSGDGESLPSGTML
                     DLSASLSTEDGLGPWNIIVKLTGNGSDLPQVSINAASSNESAPMNIASVVYRRTGSSA
                     ILRGNFTITSGKWTAPGPIVVTNRDKAFIDANISFNTSNERESCGGTFTGSITYQVPV
                     QNDEGGFEAQDWTATEGTVSYTVS"
     gene            1232..2188
                     /locus_tag="PK077_10425"
     CDS             1232..2188
                     /locus_tag="PK077_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24754.1"
                     /translation="MAASEDACFIKTFRAKIVPERLRSFVMPADGIVSNWIPADGRVQ
                     KDTIIATVNEDEIELERKELEVKILKDRIAKEEELSKLEKQLEEIEFYSSLTREERQY
                     ASKQAEGGERAIRALKDKIELSKKELSLVEDKPRLDFRKKEEKYILRMPFDGKLQYQF
                     SIPPDNSVALYMDAASPIATVCDDSSYYLTISISDPDLTNLPPESLSLSVPLGDGSAL
                     KGVFAFKRVEKNVSGGGELLAYFFRLPRQEHARAHSMLGSNCTARLYYMPQGEVFHLN
                     KVRLASLPEGKKCSSWQELLEKVHPDLELVVNGETELIVRKK"
BASE COUNT          488 a          547 c          650 g          509 t
ORIGIN      
        1 cggaattgga actcctgggc aaggaccgtc cgtccgtcaa atccttctat gtcaatgacc
       61 gcgttgtgat gctggatgaa tctgccgaaa tccagcgtgt cagccgggat gccctggaca
      121 ggccggaaat ttttctggcc cgccgccagc ctctgccctg gggaggctcc ggcctgtccc
      181 cggtggattc cggatacagc gcggatgacg gaacggtccc caccccatgg aaggattccc
      241 tgttcctgtt cctgatgggg gctgcccccc tgctggtcac gggccgcgtc ctcaagcgca
      301 ggctggaaag ggccggatga cgtttgctgc ctgcggggat tggcgtctgg attttccgcc
      361 gcaaagcgga aaatccggcg cgcgtccggg gaatggcaga ggaccgtaag ccccggtgca
      421 tcccgcgaaa aaacggaagg cccctccaaa tattctttta tttatgtgtt gacgcgccgg
      481 acggaatcac tatccttgaa gaaaatcttt ttctttatgt tttcccgatt gacgaacagg
      541 tgcgtattat cttccctttc cgcggcagct ttgtcccttg gactcttttc ctgcggaggc
      601 agcggcagca gcggtgacgg ggaaagcctt ccttccggca ccatgctgga cttgagcgct
      661 tctctttcca cggaagatgg gctagggccg tggaatatta tcgtcaaatt gacgggcaat
      721 ggttcggacc ttccccaggt aagcattaac gcggcaagca gcaatgaaag cgctcctatg
      781 aacattgctt ccgtagtgta ccgcaggacg ggatcatccg ctattctgag ggggaacttc
      841 acgattactt ccgggaagtg gacggctccg ggcccgattg tcgtgacaaa cagggacaag
      901 gcctttatag atgctaatat ttccttcaac acctccaatg aacgtgagag ctgcggcggg
      961 acgttcaccg gttccatcac ttaccaggtt cccgttcaga atgatgaagg cggatttgaa
     1021 gcgcaggact ggaccgctac ggaaggaacg gtgagttaca cggtatcctg agttctgatc
     1081 atttatgacg gggctttgct gccgtgaaat ggaggcaggc ctgtttgaaa ttatggtcgg
     1141 aagggcaaag aaatgtcttt ggggcatctg gctgtgctcg gggctggctc tttgtgtctg
     1201 gaatgcggaa tcggccggcg gggggactgg tatggcggca tcggaagatg cctgctttat
     1261 caagactttc cgcgccaaga ttgtcccgga gaggctgcgt tcctttgtca tgccggcgga
     1321 tggaattgtc agcaactgga ttcccgcgga cgggcgcgtt cagaaggata ctattattgc
     1381 aacagtcaat gaagatgaaa ttgaactgga gcgcaaggag ctggaagtaa aaattctgaa
     1441 ggaccgcatc gccaaagagg aagagttgtc aaagctggaa aaacagcttg aagagataga
     1501 gttttactct tccctgacga gggaggaacg gcaatatgcc agcaagcagg cggaaggcgg
     1561 ggaaagggcc atccgggcat tgaaagacaa gattgagctt tctaaaaagg agttgtccct
     1621 ggtggaagac aagccgcgtc tggattttcg caagaaagag gaaaaatata tcctcagaat
     1681 gccttttgac gggaaactgc agtaccagtt ttccattccg ccggataaca gcgtagccct
     1741 gtacatggat gccgcgtccc cgattgcgac cgtgtgcgat gactcctctt attatctgac
     1801 catttccatt tcggaccctg atttgacgaa tctgccgccg gaatcccttt ccctgtccgt
     1861 ccctctgggc gacggttccg ccctgaaggg ggtttttgcc ttcaagcggg tggagaagaa
     1921 tgtttccggc ggcggggaat tgctggcgta ttttttccgt ctgccccgtc aggagcatgc
     1981 gcgcgcccat tcgatgctgg gttccaactg cacggcccgt ctttattaca tgccgcaggg
     2041 agaagtgttt catctcaaca aggtgaggct ggcttccctg ccggaaggga aaaaatgttc
     2101 gtcctggcag gaacttcttg aaaaagtgca tccggacctg gagctggtgg tgaatggaga
     2161 aacggaattg atcgttcgaa aaaaatgaaa ttgc
//
LOCUS       DAOMSU010000342         2168 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5365_length_2168_cov_2.878845, whole genome shotgun sequence.
ACCESSION   DAOMSU010000342 DAOMSU010000000
VERSION     DAOMSU010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5365_length_2168_cov_2.878845"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="PK077_10430"
     CDS             <1..344
                     /locus_tag="PK077_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24755.1"
                     /translation="YDPVIKKGRPQSYKKLFQELDNDSQESANAFIKYFNEGIPEDAP
                     VSPAIHLILILCVCEVGRGYGQAPDNMLAYAHDLIDYPNLTWSSLLDTAGSSPDFAFL
                     CKGDSDEDYRE"
     gene            complement(685..1338)
                     /locus_tag="PK077_10435"
     CDS             complement(685..1338)
                     /locus_tag="PK077_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRN24756.1"
                     /translation="MITAANLHRSYSIGKKSIEILHGVDLHIAAGEKVFLCGPSGAGK
                     TTLMYTLAGLETPQEGKVMIDGTDIYALSATARSVFRNRNMGFIFQNYLLMPELTALE
                     NACLASSIGRRPRVEYVRELMERVGLSHRLNHLPSELSGGEQQRVAIARALANDAPII
                     FADEPTGNLDRKNGAEVLDLLFGLADESRKTLVIVTHDEHLARRGDRIITIMDGQAV"
     gene            complement(1369..>2168)
                     /gene="panC"
                     /locus_tag="PK077_10440"
     CDS             complement(1369..>2168)
                     /gene="panC"
                     /locus_tag="PK077_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HRN24757.1"
                     /translation="HHRKHDHVVLVPTMGALHAGHRALLEQARKLAGEDGVVVASIFV
                     NPIQFNNSSDLQTYPRTPEKDLEVCEGAGVDYVFSPASEEMYAGERSISVEEGFLSAT
                     LCGASRPGHFSGVCTVLAKLFNLVQPTDAIFGKKDYQQLAIIRRMVRDLDFPVRIHGA
                     EIVRHSNGLAYSSRNARLTPEQKEQAVVIRQAMLQARDEFQAGGDVSKVKEHAAAMIE
                     GVPGTSIDYLEIVDAETMQPLAVNRKPALMAAAVYFGDVRLIDNIEL"
BASE COUNT          496 a          641 c          572 g          459 t
ORIGIN      
        1 aatatgaccc ggtaataaaa aaaggaaggc cccaatccta taaaaaactc tttcaggaat
       61 tggataatga cagccaggaa tctgcaaatg catttatcaa atatttcaat gagggaatcc
      121 cggaagacgc tcctgtatca ccagccatac acctcattct catcttatgc gtctgtgaag
      181 taggacgtgg atacggacag gcgccggaca atatgctggc ttatgcacac gatctcattg
      241 attaccccaa tctcacatgg agttcactgc tggacacagc gggaagttcc ccggatttcg
      301 catttctatg caaaggtgat tccgacgaag actacaggga ataatgtatg atcgccgtca
      361 tcggcagaag agacagcggc agacgggctc ggacaattta gaaacatagg cgtcccttcc
      421 aatctgccgt ctgtctcaat cctgcggaag tcagaagtta gagcaccagc ttttgacttc
      481 cgccattttc gggccaaaac ctggccgccg tcatgataac agacactgct ttttccggaa
      541 tccatactat catcagcagg gagaccatcc ggaaacgccg gattaccgac gtatggctca
      601 ggtggttcgg ggcgtacacc aagccgtcct gcatcttgcg gcggacatca aataaaacgc
      661 aggaagtccc tacgcccggc agcctcagac ggcctgcccg tccataatcg taataatgcg
      721 gtcccctctc cttgccagat gttcgtcatg cgtcacaatc accagcgttt tgcgggactc
      781 gtccgccagg ccgaataaaa gatccagcac ctccgctccg ttcttccggt ccaaatttcc
      841 ggtgggttca tccgcaaaaa taatgggggc gtcgttcgcc agggcgcggg caattgccac
      901 gcgctgctgt tccccgccgc tcagctcgct gggcagatgg ttcaggcggt gggacaagcc
      961 cacgcgctcc atcagttccc tcacatattc cacgcgcggc ctccggccga tggaggaagc
     1021 cagacaggcg ttttccagtg ccgtcaattc cggcatcagc aggtaattct ggaaaataaa
     1081 ccccatgttc cggttccgga acacggaccg ggccgtggcg gacaaggcgt aaatgtccgt
     1141 accgtcaatc attacctttc cctcctgagg cgtttccaat ccggccagcg tgtacatcag
     1201 cgtggtcttt ccggccccgc tgggtccgca caggaacacc ttctctccgg cggcgatgtg
     1261 caaatccacc ccatgcagga tttcaatgga cttcttgccg atggaatagc tgcggtggag
     1321 gttggcggcg gtaatcacgg ggcgcagtat aagaaaatcc tcgccggatt aaagttctat
     1381 gttgtcaata agacgcacgt cgccgaaata gacggcggcg gccatcaggg ccggcttccg
     1441 gttcactgcc agcggctgca tggtctccgc atccacaatc tccagataat cgatgctcgt
     1501 gccgggcacc ccttcaatca tggcggcggc atgctccttc actttggaga cgtcccctcc
     1561 ggcctggaac tcgtcccggg cctgaagcat ggcctgccgt attaccacgg cctgttcctt
     1621 ctgctccggc gtcaggcggg cattccggga ggaataggcc agcccgttac tgtgccttac
     1681 aatttccgcc ccgtgaatgc gcaccggaaa atccaggtcg cgcaccatgc ggcggataat
     1741 cgcaagctgc tggtaatcct tcttgccgaa aatggcgtcc gtgggctgca ccaggttgaa
     1801 cagcttggca agcaccgtgc agacaccgga aaaatgcccc gggcgggacg cgccgcacaa
     1861 tgttgcggac aaaaagcctt cttccacgga aatgctgcgt tctccggcat acatttcttc
     1921 agaagcaggg gaaaacacat aatccacgcc tgcaccttcg cacacctcca aatccttttc
     1981 aggagtccgg ggataagtct ggaggtcgga ggaattgttg aactggatag gattgacgaa
     2041 aatactggca accactacgc catcctcccc ggccagtttg cgcgcctgtt ccagcagagc
     2101 gcggtgtccg gcgtggaggg cccccattgt agggacgaga accacatggt catgcttgcg
     2161 gtgatgct
//
LOCUS       DAOMSU010000343         2157 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5389_length_2157_cov_2.628449, whole genome shotgun sequence.
ACCESSION   DAOMSU010000343 DAOMSU010000000
VERSION     DAOMSU010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5389_length_2157_cov_2.628449"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="PK077_10445"
     CDS             <1..1068
                     /locus_tag="PK077_10445"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRN24758.1"
                     /translation="QTGTGKTAAFALPLLTRIRPIGKPQILVLEPTRELADQVAESFA
                     EYGEFTGLKVALLYGGVGYGKQTEDLKKGADIVVATPGRLVDHFYRCTMRFGEVKALV
                     LDEVDRMLDMGFLPIVRKIVNLCPWEGRQTLFFSATMPPVIAGFAKWCLTDPAEVTIA
                     RREVAATISHAFYPVALDQRDELLLALLKGTDFRSVMIFTRTRKEADAVCGMLKHHGY
                     RGEVAVMHSDIPQKERMEALKGFKSGKYDILVATDVAARGIDISGVTHVINYRVPENA
                     EDYVHRIGRTGRAEASGDAFTIMTADELDFAAAVENFIGKPIERKKLDGFNYTYTALL
                     EDKPVKSVRKPKPAGPKRRRR"
     gene            1118..1621
                     /gene="ruvC"
                     /locus_tag="PK077_10450"
     CDS             1118..1621
                     /gene="ruvC"
                     /locus_tag="PK077_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRN24759.1"
                     /translation="MRILAIDPAIRNTGYAVVEGDYRRARALDYGTLSIPRSVSQSGC
                     LLAIKQHLGNLIDKWNPDEMAVERIIYVQSHQTAITMGAAKAAVVIAAAEAGLRIMEY
                     SPKSVKLSVVGRGAAQKTQVAFMVRALLELRETPESDAADALAIGLTHLFSADPLKAH
                     MMERKYI"
     gene            complement(1715..>2157)
                     /locus_tag="PK077_10455"
     CDS             complement(1715..>2157)
                     /locus_tag="PK077_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24760.1"
                     /translation="AVPSTDDYNQSQFWIGNFPEGQYVVHLSRIVAVAQHRYILDNAC
                     MVHEVTLITLGNTPTKFYYLETLGQNSGINAIKNVTDRAKELGAGAVNRATGGSIDPD
                     TTVAKAYPNTNTVEFRVATLEQLNKMYASLLKAWQDGKGRTFKP"
BASE COUNT          465 a          615 c          627 g          450 t
ORIGIN      
        1 cagacgggga cggggaaaac cgctgctttc gccctcccgc tgctgacaag gattcggccc
       61 atcggcaaac ctcagatact ggtgctggaa cccaccaggg aactggccga ccaggtggcg
      121 gaatcctttg ccgaatacgg tgaattcacc gggttgaaag tagcgttgct gtatggcggc
      181 gtggggtacg gaaagcagac ggaagatctg aaaaaagggg cggacatcgt tgtggccact
      241 cccggccggc tggtggacca cttctaccgc tgcaccatgc gcttcggaga agtcaaggcc
      301 ctggttctgg atgaagtgga ccgaatgctg gacatggggt tcctgcccat tgtccgtaaa
      361 atcgtcaacc tttgtccgtg ggaaggaagg caaaccctct tcttctccgc caccatgcct
      421 ccggtcatcg cgggatttgc caaatggtgc ctgacggacc ctgcggaagt taccatcgcc
      481 cggcgtgaag tggccgccac catcagccat gccttttatc cggtagctct ggaccagcgg
      541 gatgaactgc tgttggccct gctcaagggg acggacttcc gttccgtcat gattttcacc
      601 cgcacccgca aggaggcgga cgcggtatgc ggcatgctca agcatcatgg ctaccgcggg
      661 gaggtggccg tcatgcactc cgacattccc cagaaggaac gcatggaggc gcttaaggga
      721 ttcaagagcg gaaaatacga tattctggtg gctacggatg tggcggcgcg cggcattgac
      781 atcagcggcg tgacccacgt catcaattac cgcgttccgg aaaacgcgga agactatgtg
      841 caccgcatcg gccgtaccgg ccgcgcggaa gcttccgggg atgcgttcac gatcatgacg
      901 gcggatgagc tggactttgc tgcggctgtg gaaaatttca ttgggaaacc cattgaacgc
      961 aaaaaactgg acgggttcaa ctacacgtac accgccctgt tggaagacaa gcccgtcaaa
     1021 tccgtccgca agcccaaacc cgcaggtccc aagcgccgca ggcgctaagg agggggggaa
     1081 acgtgatgtt tctctctgat ggaatgaaaa tcatcccatg cgcattcttg ccatagaccc
     1141 ggccatccgc aacacgggtt atgccgtggt ggagggagat taccggcgcg cgcgcgccct
     1201 ggactatgga acgctatcca ttccccggag cgtgtcccag tccggctgcc tgcttgccat
     1261 caagcagcat ttgggcaatc tcattgacaa atggaacccg gatgaaatgg cggtggagcg
     1321 catcatctat gtccagtctc atcagacggc catcacgatg ggtgcggcca aggcggccgt
     1381 ggtcattgcc gcggcggaag ccgggctgcg tattatggaa tattccccca aaagcgtgaa
     1441 gctttccgtc gtagggcgcg gggccgctca aaaaacgcag gttgccttca tggtgcgggc
     1501 ccttttggaa ctccgggaaa cgccggaatc cgatgccgcc gacgctctgg cgatcggcct
     1561 cacccacctc ttttctgcgg accctttgaa ggcccacatg atggagagga aatacattta
     1621 gcagtgatga gagatgagcg taaaaaccgc acttgcagcc cgcgaattga aatttgactg
     1681 cccggcgctg ttctctaatc actgattcaa gccgtcaggg cttgaacgtc ctccctttcc
     1741 cgtcctgcca cgccttgagc agggaagcat acattttatt gagctgttcc agggtagcta
     1801 cacggaattc cacggtattc gtattcgggt aagctttcgc aacggttgta tccgggtcaa
     1861 tgctgcctcc cgttgcccgg ttgacggcgc cggccccaag ctccttcgca cggtcagtca
     1921 cattcttgat ggcgttgata ccggaatttt gacccagcgt ttccagataa taaaacttgg
     1981 tgggagtatt ccccagggta atcagcgtta cttcatgcac catgcaggca ttgtctagaa
     2041 tatacctgtg ctgcgccacc gctacaatcc ggctgagatg caccacatac tgtccctccg
     2101 gaaaattgcc tatccagaac tggctctggt tgtagtcatc cgtggacggg acggcgg
//
LOCUS       DAOMSU010000344         2108 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5579_length_2108_cov_2.115441, whole genome shotgun sequence.
ACCESSION   DAOMSU010000344 DAOMSU010000000
VERSION     DAOMSU010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5579_length_2108_cov_2.115441"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..525
                     /locus_tag="PK077_10460"
     CDS             55..525
                     /locus_tag="PK077_10460"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRN24761.1"
                     /translation="MKAILDACCGSRMFWFDRRHPDVVFMDRREETHMLCDGRTLEIK
                     PDVVGDFRKMPFNDGAFRLVVFDPPHLIHAGESSWLAKKYGKLDQKTWREDLKSGFRE
                     CFRVLEPGGILVFKWCEDQVSTAEVLKLASHEPLFGHRRGKTVFLVFMKSTTPN"
     gene            539..1315
                     /locus_tag="PK077_10465"
     CDS             539..1315
                     /locus_tag="PK077_10465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24762.1"
                     /translation="MEFINIPTALFSSPEYIGAEPIQRATWISLLAWCCEQENGGIIE
                     GCRSWGMRRWMQTCGVTDQEISVENELYHFDGDNLIVFGYPHEIQETLKTKRKTAREN
                     GKLGGRPKKTHVETHTGTDVETEEKPTSVISETNVGTEIGTNVAPYVETYPKTVREGK
                     EGKEGIHPLTPSPCTVEEVEDHLRAAAFAGRVRLTPDQIPDCATAYWGSRDAVNWTRN
                     GIPVTKWQSDAISFATSYAVNHPPPPGNGDKDPYSNLEEL"
     gene            1338..>2108
                     /locus_tag="PK077_10470"
     CDS             1338..>2108
                     /locus_tag="PK077_10470"
                     /inference="COORDINATES: protein motif:HMM:NF012974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HRN24763.1"
                     /translation="MIDSQTLIDAEKLVLSQAMDGSQAFADLRDKGISRQTFSLPAHQ
                     QIWTALETVAGTGGTVDALTVIARLEAQGQLDAVGGHAGVVETATYGALARYKTAAAL
                     EMVTEAAKKHALLAFASRMAEAAGDQLKSAEEALDEAERGMSALRDRCGVRQTETIRG
                     AVGTIIENLQWRMNNPGAIKGISSGYRRLDLTLDGLQPGAMIVLAARPGVGKTAALVN
                     ILTNICLGGTPVGMFSLEMPKSQLLERILYGMAGINSDD"
BASE COUNT          511 a          652 c          581 g          364 t
ORIGIN      
        1 agcctgcgtc catgaaggat ggcctgttta ctgggagccg agaaaggagg gggaatgaaa
       61 gccattcttg acgcctgctg cggctcccgc atgttctggt ttgaccgccg ccatcctgac
      121 gtggtgttca tggaccgccg ggaggaaacg cacatgcttt gcgacgggcg aaccctggaa
      181 atcaagccgg acgtcgtcgg ggacttccgg aagatgcctt tcaacgacgg ggcgtttcgc
      241 cttgtggtat tcgaccctcc gcacttgatt cacgctgggg aatcatcctg gctggccaag
      301 aagtacggaa aactggacca gaaaacctgg agggaggatt tgaaatccgg cttccgggag
      361 tgtttccggg ttttggaacc gggcggcatt ctggtgttca agtggtgcga ggatcaggtt
      421 tcaaccgcgg aagttctgaa actggccagc catgaacctt tgttcggaca ccgccgcggg
      481 aagaccgtct tcctggtctt tatgaaatct acaaccccca actgacgctt ttttgattat
      541 ggaattcatc aacatcccaa cagccttgtt ttccagcccc gaatatatcg gggcggaacc
      601 catacagcgc gccacctgga tctctctgct ggcctggtgc tgcgaacagg aaaacggcgg
      661 catcattgag ggctgccgct cctggggcat gcgccgctgg atgcagacct gcggcgtgac
      721 ggatcaggaa atcagcgtgg aaaacgaact ctaccacttt gacggcgaca atctcatcgt
      781 attcggatat ccgcatgaaa ttcaggaaac cctgaaaacc aaaaggaaaa ccgctcgtga
      841 aaatggaaaa ttaggaggcc gccccaagaa aacccatgtt gaaacccaca caggaaccga
      901 cgtggaaacc gaagaaaaac ctacgtcggt tatttcagaa accaacgtag gaaccgaaat
      961 aggaaccaac gtagccccct atgttgaaac ctatccgaaa accgtaaggg aagggaagga
     1021 agggaaggaa ggaattcacc cccttacccc ctctccgtgc accgtggaag aagtcgaaga
     1081 ccatcttcgg gccgcggcct ttgcggggcg tgtgcgttta acccccgacc agataccgga
     1141 ctgcgccaca gcctactggg gaagccggga tgccgtcaac tggacccgca acggcatccc
     1201 cgtgaccaaa tggcaatccg acgccatcag cttcgccacc tcctacgccg tcaaccatcc
     1261 gccaccccct gggaacggag acaaagaccc ttacagcaac cttgaagaac tttaacaatc
     1321 aacaatttca aaaaaacatg atcgactctc agacactcat cgacgccgaa aaactggtgc
     1381 tctctcaggc aatggacggc tcccaggcct ttgctgacct ccgggacaag ggcatcagcc
     1441 gccagacatt cagcctcccg gcgcaccagc aaatctggac cgccctggaa accgtcgccg
     1501 gcacgggagg aaccgtggac gccctcaccg tcatcgcccg ccttgaagcc cagggccagc
     1561 ttgacgccgt gggaggacac gccggagtcg tggaaacggc cacctacgga gcccttgccc
     1621 ggtacaaaac cgccgccgcc ctggaaatgg tcacggaagc cgccaaaaag catgcgttgc
     1681 tcgcgtttgc ctcccggatg gcggaagctg ccggcgatca gctcaaaagc gcggaagaag
     1741 cccttgatga agccgagcgc ggcatgtccg ccctgcggga ccggtgcggc gtccgccaga
     1801 ccgaaaccat ccgcggagcc gtgggaacca tcattgaaaa cctgcaatgg cgcatgaaca
     1861 accccggagc tatcaaagga atctcctccg gataccgccg cctggacctg accctggacg
     1921 gcctgcagcc cggcgccatg atcgtgcttg ccgcccggcc cggagtcggg aaaaccgccg
     1981 ccctggtcaa catcctcacc aacatctgcc tcgggggaac ccccgtgggc atgttcagcc
     2041 tggaaatgcc gaaatcccag ctcctggaac gcatcctcta cggcatggcc ggcatcaact
     2101 ccgacgac
//
LOCUS       DAOMSU010000345         2105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5589_length_2105_cov_2.122439, whole genome shotgun sequence.
ACCESSION   DAOMSU010000345 DAOMSU010000000
VERSION     DAOMSU010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5589_length_2105_cov_2.122439"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PK077_10475"
     CDS             <1..1152
                     /locus_tag="PK077_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRN24764.1"
                     /translation="LMRCPEAELPHRVELLFQKISDAPAPLPYQRGNWHLQPAEGWKT
                     AADIGAMAPGYKNPWKERGKPFSPSVLKRFLSCPMRFWTQEALHMNEEEFLPDKEDMA
                     ANELGTMLHDVLECFCREHAVLKDGMNAASLQAAITEILEQTFRKQYGPSPLMPLLLQ
                     KRSMEQRLSVYAVQHLQALQEGWSCIAFERQVENWTLGGFPMKFRIDRIDRHADGRIR
                     VIDYKTGAASSCEKKHLEPLGRPDALSLLSPALHPYTKRRKNGKLAHARWKDLQLPVY
                     VLWASETYGGAPSAAYYNLPANPMDIGISSWDTLHDTMPGYEECALDSARSWAVELMK
                     LLYEGRGLITAEELGWPPPSYDVFKDLMTSRRESLQDLLGLRLTPHLPF"
     gene            1154..>2105
                     /locus_tag="PK077_10480"
     CDS             1154..>2105
                     /locus_tag="PK077_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRN24765.1"
                     /translation="MPPLTNMLISASAGTGKTYQLSLRFLGLLALNGGNHPERLIAIT
                     FTRKAAGEFKDRILTDLAAGATDEAGAARLKERLWAVIKGTDGEPGLWPGAPEAWKEE
                     NLHRERFLHLLHILVQNLARLNLCTIDSLFAQIASASAFELGVSGFSMIDPTAEKLAR
                     REALLSLYRECSVNRERRKDFEDAFLSGADSDAEAADAENSMMRRLDTYHELFLDVPD
                     AGMWGNPVTLGFTLEELAPPVPLEQFDSVLHDLLEQAKATPIPEKKREANTREDFISY
                     LKSFNTYTFLGKVWFRGKSGSKTDISPEKARAKFAEYWSPL"
BASE COUNT          469 a          660 c          583 g          393 t
ORIGIN      
        1 ctcatgcgct gcccggaggc ggagctgccg caccgcgtag aactgctttt ccagaaaatc
       61 agcgatgcgc ccgcgccctt gccctaccag cgcgggaact ggcatcttca acctgcggaa
      121 ggctggaaga ccgctgcgga catcggcgcg atggcgcccg gctacaaaaa cccgtggaag
      181 gaaagaggga agcccttctc cccctccgtc ctcaaaaggt tcctgtcctg ccccatgcgt
      241 ttctggacgc aggaggctct gcacatgaat gaggaagaat tcctgccgga caaagaagat
      301 atggccgcca atgaactggg caccatgctg cacgacgtgc tggaatgctt ctgccgggaa
      361 cacgccgtcc tgaaagacgg catgaacgca gcttcccttc aggccgccat cacggaaata
      421 ctggaacaaa ccttccggaa acaatacggc ccctcccccc tcatgcccct gctgctgcaa
      481 aaacgctcca tggaacagag gctttccgtt tacgccgttc agcacttgca ggcccttcag
      541 gaaggatggt cctgcattgc ctttgagcgt caggtagaga actggacact gggcggcttc
      601 cccatgaaat tccgtattga ccgtattgac cgccatgcgg acgggcgcat acgggtcatt
      661 gactacaaaa ccggagcggc ctcctcctgt gaaaaaaagc atctagaacc gctcggacga
      721 ccggacgccc tgtccctgct ttccccagcc ctgcatccct acaccaaacg acggaaaaac
      781 gggaagctgg cgcacgcgcg ctggaaagac ctgcagcttc ccgtttatgt gctgtgggcc
      841 tcggaaacct acggcggcgc tccctcagcg gcctattaca accttcccgc caatcccatg
      901 gatatcggaa tctcctcctg ggacaccctc cacgacacca tgcccggata tgaggaatgc
      961 gcgctggaca gcgcccgttc ctgggcggtg gaactgatga agcttcttta tgaaggccgc
     1021 gggctcatca ccgcggagga actgggctgg ccaccgccct cttatgacgt attcaaggac
     1081 ctgatgactt cccggaggga aagcctgcag gatcttttgg gcctccgcct caccccccac
     1141 ctgcctttct aagatgccgc ccctgaccaa catgctgatc tccgcttccg ccggaacggg
     1201 aaaaacctac cagctctccc tgcgtttcct ggggttgctg gcgctgaacg gcggcaacca
     1261 ccccgaacgc ctcatcgcca ttacgttcac ccgaaaagcg gcgggagaat tcaaggaccg
     1321 cattctgaca gacctggccg cgggggccac ggatgaggca ggcgcggcgc ggctgaaaga
     1381 acggctatgg gcggttatca aaggaacgga cggggaaccc ggtctctggc ccggcgctcc
     1441 ggaggcatgg aaggaggaaa acctgcatcg ggaacgcttc ctccatttac tgcacattct
     1501 ggtgcagaat ttggcgcggc tcaacctctg caccattgac agcctgtttg cccagattgc
     1561 ctccgccagc gcctttgaac tgggagtcag cggcttcagc atgattgacc ccacggcgga
     1621 gaaactggcg cgccgtgaag ccctgctctc cctgtaccgg gaatgctccg taaacaggga
     1681 gcgaaggaag gactttgaag atgccttcct ttccggggcg gattctgacg cggaagccgc
     1741 ggacgcggag aattccatga tgcgccgcct ggacacctat cacgaacttt ttctggatgt
     1801 gccggatgcc gggatgtggg gaaaccccgt cacactgggc ttcacactgg aggagctagc
     1861 gcctcccgtt cccctggaac aatttgactc cgtactgcac gacctgttgg aacaggccaa
     1921 agccaccccc ataccggaga aaaaaagaga agcgaatacg cgggaagact tcatcagtta
     1981 tctgaaaagt ttcaacacct acacgtttct gggtaaagtc tggttccgag gaaagagcgg
     2041 ttccaagacg gacatctccc cggaaaaggc tcgtgccaaa tttgctgaat actggtctcc
     2101 gctat
//
LOCUS       DAOMSU010000346         2095 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5622_length_2095_cov_2.565686, whole genome shotgun sequence.
ACCESSION   DAOMSU010000346 DAOMSU010000000
VERSION     DAOMSU010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5622_length_2095_cov_2.565686"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="PK077_10485"
     CDS             complement(<1..429)
                     /locus_tag="PK077_10485"
                     /inference="COORDINATES: protein motif:HMM:NF014852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 20 zincin-like fold
                     domain-containing protein"
                     /protein_id="HRN24766.1"
                     /translation="MNGFQLLQCGLSVAAFLAGSALAATSAVYPSPQQSKFTTQTVAF
                     SGKPSVTIRSAKAGGSKLLEGVPEKSGAYKLVISPQGKVGIGAHDERGAFYAMQTLRQ
                     LGTKAGGEGVILPVGEIIDWPDIEFRGTVEGFYGTPWSHEA"
     gene            731..1651
                     /locus_tag="PK077_10490"
     CDS             731..1651
                     /locus_tag="PK077_10490"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRN24767.1"
                     /translation="MLIPFLCLLGLFCSLQAQDAPILKIAALHPVLGDMARSIGGSRV
                     QVADLLRPNGNLHSFEPAPQDIAAAGQASLVLASGKNLEPYLPKLKDALGGNVQILDL
                     GASVPDVPVEEDTADHDHEHDGDCCAHGPNDPHWWHTPANMKRAARTLAAMLTRLDPA
                     HEQDYKAGLARWNRKMDQLSSWARKELADIPEKDRVLVTGHAAMNHFCREFGFRSISI
                     QGISREDEGNSAQLASTLNKLRSAGVKALFPEYSSNPKSLTEIAKSLNIPVARPINTD
                     GLAPEGHTFESMFRQNTGIIKEALSPSPRP"
     gene            1648..2082
                     /locus_tag="PK077_10495"
     CDS             1648..2082
                     /locus_tag="PK077_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24768.1"
                     /translation="MTSTPLRQFFPTLGVVILLGFFLYNMTAPSPGTQGTAHAAPAAP
                     TTDVPTVITVRCAHRPEALSISCGGRLLWQPETPGLHEETECGLPLKDGSVILTVSAR
                     WPENTPDTPVTLELEPEGKPTVSTTRWSFGPSLNDSYSFSWK"
BASE COUNT          463 a          709 c          550 g          373 t
ORIGIN      
        1 ggcttcatgg ctccagggag tgccgtaaaa accttccacc gttccgcgga attcgatgtc
       61 cggccagtct atgatttccc caacgggcaa aatcacgcct tccccgccgg ccttcgttcc
      121 cagctgccgc agcgtctgca tggcgtaaaa cgccccccgt tcgtcatggg ctcctatgcc
      181 cacctttccc tgcggagaaa tcaccagttt gtaggctccg gatttctccg gaactccctc
      241 cagcagcttg ctgccgcccg ccttggcgga acgtatggtc acggacggct tcccggagaa
      301 agcaaccgtc tgggtggtaa atttggactg ttggggagac ggatatacgg cagaagtcgc
      361 cgccagggcg gaaccggcca gaaaggccgc cacgctcaag ccacattgga gaagttgaaa
      421 accattcatg atgatgcgcg ataatggaac cagaaaacac tttccacctc aagaaaaaac
      481 ctctctccgc catcagaccg tcgtcaccag tggaagaaag cccccggagc ccgcacggga
      541 accccggcca ggctggacgc caagggcgcc gcatccccct tcccctgcca ggagttcacc
      601 acaacgctga aaaacaaaaa aagagccgtg cggactccct gaaaagtcag cgtcatcctg
      661 ctttagaaga caaatttgac cattgcatca cttgaaggga aggttcatca tgcggaacat
      721 gctccggtgc atgttgattc cttttctgtg tcttctgggc ctgttctgtt ctcttcaggc
      781 ccaggatgcc ccaatattga aaatcgcggc tctgcacccc gttctgggag acatggcccg
      841 ctccattggc ggcagccgcg tgcaggtggc ggacctgctg cgccccaacg gcaacctgca
      901 cagttttgaa cccgcgccgc aggatattgc cgccgcggga caggcgagcc tggtgctggc
      961 ctccggcaaa aacctggagc cgtaccttcc caaattaaag gacgccctgg gaggcaatgt
     1021 gcaaattctg gatcttggag cctccgttcc ggacgttccc gtggaggaag acaccgcaga
     1081 ccatgaccat gaacacgacg gagactgctg cgcccacggc cccaacgacc cccactggtg
     1141 gcacaccccg gccaatatga aacgcgccgc ccgcaccctg gccgcaatgc tcacgcggct
     1201 ggacccggcc catgaacagg actataaagc cggcctggcc cggtggaaca gaaagatgga
     1261 ccagctttcc tcctgggcca gaaaggagct tgcggacatt ccggaaaagg accgcgtcct
     1321 ggttacgggg catgcggcca tgaaccattt ctgccgggaa ttcggcttcc gcagcatcag
     1381 catccaggga ataagccgtg aagacgaagg caactccgcc cagctggcct ccacgctgaa
     1441 caagctgagg tccgccgggg tcaaggccct gttcccggaa tactcctcca accccaagag
     1501 ccttacggaa atcgccaaat ccctgaacat ccccgttgcc aggccaatca ataccgacgg
     1561 gctggcccct gaaggccaca cgttcgaatc catgttcaga caaaacacag gcatcatcaa
     1621 ggaagccctt tccccgtctc ccagaccatg accagcactc ccctgcggca gttttttccc
     1681 actctgggag tcgtcatcct gctgggattc ttcctttaca acatgacggc gccttctccg
     1741 ggaacacagg gaacggcgca tgccgcccct gcggcgccaa ccacggatgt tcccacggtc
     1801 atcacggtca gatgcgccca ccgtccggaa gccctttcca tatcctgcgg cggccgcctt
     1861 ctctggcagc cagaaacgcc cgggctgcat gaagaaacgg aatgcggcct gccgttgaaa
     1921 gacggctccg tgatcctcac cgtttccgcc cgctggccgg aaaacacgcc ggacaccccc
     1981 gtcacgctgg aactggaacc ggaggggaaa cctaccgtct ccaccacccg ctggtccttc
     2041 ggcccttccc tgaacgacag ctactccttc tcatggaaat gaacagccca gcccc
//
LOCUS       DAOMSU010000347         2094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5626_length_2094_cov_2.803335, whole genome shotgun sequence.
ACCESSION   DAOMSU010000347 DAOMSU010000000
VERSION     DAOMSU010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5626_length_2094_cov_2.803335"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..>2094
                     /gene="scpA"
                     /locus_tag="PK077_10500"
     CDS             28..>2094
                     /gene="scpA"
                     /locus_tag="PK077_10500"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HRN24769.1"
                     /translation="MSNIPDFASASYPEIKAGAPSPASETETWMTNEQIPVPPTYSES
                     VYDDCLHLDFTAGVAPNLRGPYATMYVARPWTVRQYAGFSTAEESNAFYRRNLAAGQK
                     GLSIAFDLATHRGYDSDHPRVVGDVGKAGVAVDSILDMEILFKGIPLDKMSVSMTMNG
                     AVLPVLAFYIVAAQEQGCTLDQLSGTIQNDILKEYMVRNTYIYPPDPSMRIIADIFEF
                     TSKFMPKFNSISISGYHMQEAGATADLEMAYTLADGLEYVRTGIKAGIDIDAFAPRLS
                     FFWAQGKNYFMEVAKMRAARVLWAKLIKQFNPKNPKSLALRTHSQTSGWSLTEQDPFN
                     NVTRTCIEALAAACGHTQSLHTNSLDEAIALPTDFSARIARNTQLHLQDETTICKVID
                     PWGGSYYVEYLTNELIRKGWAHLQEVEKLGGMAKAIETGLPKMRIEEAAARRQAAIDS
                     GKEPIIGVNKYRLEQEAQIDILEVDNTTVREAQIARLQKLRAERDSAACEAALEALSE
                     CARTGEGNLLDLAIKAAKARASLGEISSALEKHFGRHKAPIKLITGVYAQSYGQDPLV
                     EEVRKMTDDFAERTGRRPRILVAKMGQDGHDRGAKVVSSAYADLGFDVDVGPLFQTPE
                     ETAKMAIENDVHMIGMSSLAAGHKVLLPALVEELARQGRPDILVFCGGVIPAQDYDFL
                     KENGAVA"
BASE COUNT          452 a          698 c          559 g          385 t
ORIGIN      
        1 ctaacaacca ccaacctatt ccacccaatg agcaatattc ctgattttgc atccgcctcc
       61 tatcctgaaa tcaaggctgg tgccccgtcc ccggcttccg aaacggaaac ctggatgacc
      121 aatgaacaaa ttcccgtgcc gcccacctac tcggaaagcg tgtacgacga ctgcctgcac
      181 ctggacttta cagccggtgt ggctcccaac ctccgcggac cgtatgccac catgtatgtg
      241 gcgcgcccct ggacggtacg ccagtacgcc ggcttctcca cggctgagga atccaatgcc
      301 ttctaccgcc gcaacctggc ggcgggccag aagggccttt ccattgcctt tgacctggcg
      361 acgcaccgcg gctatgactc cgaccacccc cgcgtggtgg gtgacgtggg caaggccggc
      421 gtggccgtgg actccatcct ggacatggaa atcctcttca agggcattcc cctggacaaa
      481 atgtccgtct ccatgaccat gaacggcgcc gtgctgcctg tgctggcctt ctacatcgtc
      541 gccgctcagg aacagggttg cacgctggac cagctctccg gcacgatcca gaatgacatt
      601 ctcaaggaat acatggtgcg caacacctac atttacccgc ctgatccctc catgaggatt
      661 attgcggaca tctttgagtt cacctccaag ttcatgccca agttcaactc catttccatc
      721 tccggctacc acatgcagga agccggcgct acggcggacc tggaaatggc ctacaccctt
      781 gcggacggct tggaatatgt ccgcaccggg atcaaggcgg gtattgacat tgacgccttc
      841 gcgccgcgtc tttccttctt ctgggcccag ggcaagaatt acttcatgga agtggccaaa
      901 atgcgcgccg cccgcgtgct gtgggccaaa ctcatcaagc agttcaaccc caagaacccg
      961 aagtcactgg ccttgcgcac gcactcccag acttccggct ggtccctcac ggaacaggac
     1021 ccgttcaaca acgtcacccg cacctgtatt gaggccctgg ccgccgcctg cggccatacg
     1081 cagtccctgc acacgaactc cctggacgaa gcgattgccc tgccgacgga cttttccgcc
     1141 cgcatcgccc gcaacaccca gcttcatctt caggatgaaa ccaccatctg caaggtaatc
     1201 gacccgtggg gcggctccta ctatgtggaa tacctgacca atgagctcat ccgcaagggc
     1261 tgggcccacc ttcaggaagt ggaaaaactc ggaggcatgg ccaaggccat tgaaaccggg
     1321 ctgcccaaga tgcgcattga agaagccgca gcgcgacgcc aggccgccat tgactccgga
     1381 aaggaaccca tcatcggcgt taacaaatac cgcctggaac aggaagccca aatcgatatt
     1441 ctggaagtgg acaacaccac cgtccgggaa gcccagatcg cccgcctgca gaaactgcgc
     1501 gcggaacggg actccgccgc gtgcgaagcc gcgctggaag ccctgtccga atgcgcccgc
     1561 acgggggaag gcaatctcct tgaccttgcc atcaaggcgg ccaaggcccg cgcctccctg
     1621 ggtgaaatct cctccgccct ggaaaaacac ttcgggcgtc ataaagcacc aatcaaactc
     1681 attaccggcg tgtacgctca atcctatggt caagatccgc tggtggagga agtccgcaag
     1741 atgacggatg acttcgccga acgcacgggg cgtcgtcccc gcatcctcgt cgccaaaatg
     1801 ggccaggacg gccatgaccg cggcgccaag gtggtctcct ccgcctatgc cgacctcggt
     1861 tttgacgtgg acgtgggccc gctcttccag acgccggaag aaactgccaa aatggcgatt
     1921 gaaaacgacg tgcacatgat cggcatgagc tctctggccg cgggccacaa ggtgctgctt
     1981 cccgccctgg tggaagaact tgccaggcag ggccgtccgg atatcctggt cttctgcggc
     2041 ggtgttattc ccgcccagga ctatgacttc ctgaaggaaa acggagctgt ggcc
//
LOCUS       DAOMSU010000348         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5639_length_2090_cov_2.131204, whole genome shotgun sequence.
ACCESSION   DAOMSU010000348 DAOMSU010000000
VERSION     DAOMSU010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5639_length_2090_cov_2.131204"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="PK077_10505"
     CDS             complement(<1..282)
                     /locus_tag="PK077_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24770.1"
                     /translation="MKATVSIGGVIHPSLQRTLSRVHKSVGFLVSKYKALGAITLTGA
                     VAGVASLGAITAKSVGQAIELQKEMSNVATLLDGDVHKRVGELQKDVLDL"
     gene            complement(423..800)
                     /locus_tag="PK077_10510"
     CDS             complement(423..800)
                     /locus_tag="PK077_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HRN24771.1"
                     /translation="MEKIELQYPFQINGQDIREIEYDFGEFTANDYFTAMKNRRGYSP
                     EVTPVNDYGMNYSIGVQVILASNKGKGWTVEDFDRLRGSDVSKVMLVGLNFFGATPEE
                     QANETSEGRSESTPSDSTLPEKS"
     gene            complement(832..1341)
                     /locus_tag="PK077_10515"
     CDS             complement(832..1341)
                     /locus_tag="PK077_10515"
                     /inference="COORDINATES: protein motif:HMM:NF016846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HRN24772.1"
                     /translation="MKFQELKGACIASSVYAKGEQIGYNIPIKLPEVTPVIVEIQAAG
                     GKLELPVWQQVEAMEASITKTGVSKEFLESLTPEPFDLISNVAQQSVSADGTSTAQHI
                     KAFMRVIPKSAPGVEITAGEASENELPFTVLSYQLYVDGNKYLDIDVVKGVCWINGKD
                     YSESIRSML"
     gene            complement(1374..>2090)
                     /locus_tag="PK077_10520"
     CDS             complement(1374..>2090)
                     /locus_tag="PK077_10520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24773.1"
                     /translation="DIPLENAGTIEAAKAWKKANGYTSEYSKVFWPQAKTSTGEIYHL
                     STLAVWVMQMVDNDHDGVPGETCSNKAVPVICQYFGDGAKNRGFDQETGNALNENGIS
                     TVAPFNGKWVLWGGHTAAYAFGVTSDALHIFDTNIRMLEHIVNSFQKEWAPRVDKPMT
                     IQLRDEIIHRENDKLAGYVAQGYLVGNPECIFLPSENADSDMMNGDFRWNLSATPTPQ
                     FKSGTISVSYTDSGLSVYSA"
BASE COUNT          450 a          569 c          515 g          556 t
ORIGIN      
        1 aagatccaga acatccttct gcagctctcc aaccctcttg tgaacatcgc cgtccaaaag
       61 ggttgccaca ttggacatct ctttttgaag ttcgatggcc tgaccaacgg actttgccgt
      121 aatcgcgccg agggaggcta caccggcaac cgcccccgtg agtgtaatgg cgcccagggc
      181 tttgtacttg gacaccagaa atccaacact tttatggaca cgggaaagcg tccgttgcag
      241 agacgggtga attaccccgc cgatgcttac ggtcgctttc atagtctttt cccgcgccat
      301 aatggtctat ctcctttttt tacgccggtg cgcgttctgt ttttccgcct tctggcgttc
      361 cttctcggca tcttctgcgg cttcctgaaa ctccgtccag aaatcaacga gaggcatttc
      421 catcagctct tttcgggtag cgtggaatcg ctcggagtag attcggatcg cccttcggag
      481 gtctcgttcg cttgctcctc gggcgtggct ccaaaaaaat tcagaccaac cagcatgacc
      541 tttgaaacgt cacttccccg gaggcggtcg aagtcttcca cggtccatcc cttcccctta
      601 ttgctcgcca gaatgacttg cacgccaatc gaataattca ttccgtagtc attcacggga
      661 gtgacctcag gagaatatcc gcggcggttc ttcatggcgg tgaaataatc attggccgtg
      721 aactccccga aatcatattc aatttcccgg atatcctgac cgttgatctg aaacggatac
      781 tgtaattcta ttttttccat aatatggtgt gttgatggtt aattgttgat attaaagcat
      841 gctgcggatg ctttcagagt agtctttgcc gttgatccag cacacgcctt tgacgacatc
      901 aatgtccaga tacttgtttc cgtccacgta aagctgatag gagaggacag taaacggaag
      961 ttcgttttcg ctggcttctc ccgcggtaat ctccactccg ggtgccgact tgggaatcac
     1021 gcgcatgaac gccttgatat gctgggcggt actggtaccg tcagcggaca cgctctgctg
     1081 ggcgacattg gaaatcagat caaacggctc cggagtaagg gattccagga attccttgct
     1141 gacgcctgtc ttggtaatgg atgcctccat agcctccacc tgctgccaga ctggcaattc
     1201 cagtttgccg ccagccgcct ggatttctac tataacgggg gtaacttcag gcagcttgat
     1261 ggggatattg taccctatct gttccccttt cgcgtaaacg gaggaagcaa tacaagcccc
     1321 tttcaattct tgaaacttca tgaatagtaa tcctttctat attatattga gtgttatgct
     1381 gaataaacgg acagaccgga atccgtgtag gaaacggaga tggtgccact cttgaactgt
     1441 ggcgtgggcg tggcggacag gttccagcgg aaatctccgt tcatcatgtc ggaatcggca
     1501 ttctcgctcg gaaggaaaat gcactccgga ttcccgacaa ggtatccttg ggccacatac
     1561 ccggccagct tgtcattttc ccggtgaatg atctcgtccc gaagctgaat agtcatcggt
     1621 ttgtcaaccc ttggtgccca ttccttctgg aagctgttca cgatgtgttc cagcatccgg
     1681 atattcgtgt caaagatatg gagggcatcg gacgttaccc cgaacgcata ggctgccgta
     1741 tggccgcccc aaagaaccca cttcccgttg aatggcgcca cggtggagat gccgttttcg
     1801 ttgagtgcat tccccgtctc ctggtcaaaa cctctgttct tggcaccatc gccgaaatac
     1861 tggcaaatga ccggcaccgc cttgttggaa caggtctcgc cgggaacgcc atcgtggtca
     1921 ttatccacca tctgcatcac ccaaacggcc agtgtggaaa gatggtaaat ctctccggtg
     1981 ctggtctttg cctggggcca gaacaccttg gaatattcag atgtgtaccc gtttgccttc
     2041 ttccaggctt tggcggcttc tatggttcct gcattttcca aggggatatc
//
LOCUS       DAOMSU010000349         2068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5721_length_2068_cov_2.355191, whole genome shotgun sequence.
ACCESSION   DAOMSU010000349 DAOMSU010000000
VERSION     DAOMSU010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5721_length_2068_cov_2.355191"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="PK077_10525"
     CDS             <1..641
                     /locus_tag="PK077_10525"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRN24774.1"
                     /translation="LEVFEQMRGALTPEHIILNHSTIDLATVGKLSLMSSSIGCTYVD
                     CPFTGSRLPAEKGELVYYVGTDSTTLDRIRPVLEHSSRDILHLGGIGAGTVVKLVTNM
                     VSATMVQSLSEALAISQAYGISPEMLGQAISRNVIASPLAALKLPLMAKRDFSTHFSL
                     DNMRKDSIYAQELAAEKGIHPPIAALVSSIMGKLCREGHAEEDFSCLAEQFD"
     assembly_gap    807..864
                     /estimated_length=58
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <865..>2068
                     /locus_tag="PK077_10530"
     CDS             <865..>2068
                     /locus_tag="PK077_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24775.1"
                     /translation="VVPPSPSAKAAAGTEEQPAKRQPFTSVSTSKQFHVTGMDSLLAG
                     AIATRADAIRTALLKVLKQPDEWKNGINIRLVGEPGSPVPANPVLMQTVIVGNTLSYS
                     IFIHLGHGINQDSLRRAIVSTLLYEMMLRTVDPEGLPDNVTLPPWLIAGLEQAVLWHN
                     NEADRAMYSTLFQQSEIMTPEEILNCREPEKELDATSYAAYQTSCGALVLCLLNQENG
                     PEGMKQLLDQAILGNDDPRNLIKRHFPQLSLTPTSLHKWWTLQLSRMATPPLTETLTI
                     TETERHLQEALTLVKYDPDTRTTVTFPLDDLEQARSLPDLNRQLSNVAGSLLNLSRRS
                     FPSYRPVIVEYAKLVALMQTGKVNSREAVPKISQLREIRNLSMKTARRVRDYMDWYEI
                     NTRSGTGKT"
BASE COUNT          455 a          628 c          515 g          412 t
ORIGIN      
        1 gtctggaagt gtttgaacaa atgagagggg ccctgacacc ggagcatatc attctgaacc
       61 attcaaccat tgacttggcg accgtcggca aactctccct gatgagttcc tccatcggct
      121 gcacgtatgt ggattgcccg ttcaccggct cccgtttgcc cgcggaaaaa ggggaactgg
      181 tttactacgt tggaacggat tccaccactc tggaccgcat acgccccgtg ctggagcaca
      241 gttcccggga catcctgcat ctgggaggca ttggagccgg caccgtcgtc aaactggtca
      301 ccaacatggt ctccgccacc atggtgcaga gtttgagcga agccctggcc atctcccagg
      361 cgtacggcat ttccccggaa atgctgggcc aggccatttc ccggaacgtc attgcctccc
      421 ctctggccgc attgaagctg cccctgatgg caaagcgcga cttttccact catttctccc
      481 tggacaacat gaggaaagac agtatttacg ctcaggaact tgccgcagaa aaaggaatcc
      541 accctcctat tgccgccctg gtttcctcca tcatgggtaa gctgtgccgg gaaggtcatg
      601 cggaagagga tttcagctgt ctggcggaac aattcgacta actttttccg cggatgaaat
      661 tcaggggaac atgcgtcttt ctgttgacgg ctcttcttct gccccctgct gtctgggggc
      721 aggaacagaa aggggctggc gatgtttctt ccgcggaagt gcggctaccg gaagaagaag
      781 aaatatccgt ccaggatgcg gcttttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      841 nnnnnnnnnn nnnnnnnnnn nnnnccgtcg ttcccccttc accttcggca aaagccgccg
      901 ccggaacgga ggaacagccc gccaaaaggc agccgttcac cagcgtttcc acctccaagc
      961 aattccacgt caccgggatg gactccctgc tggccggggc catcgccacc cgggcggatg
     1021 ccatacgcac cgcgctgcta aaagtcctca agcagcctga cgaatggaaa aacggcatca
     1081 acatccgcct ggtaggagag ccgggaagcc ccgttccggc caaccccgtc ctcatgcaaa
     1141 ccgtcattgt aggcaatacc ctctcctata gcattttcat tcatctgggc cacggcatca
     1201 accaggacag tctgcgccgc gccatcgtct ccaccctgct ttatgaaatg atgctgcgca
     1261 cggtggaccc ggaaggcctg ccggacaacg tcacgctgcc cccctggctg attgccgggt
     1321 tggaacaggc tgttctgtgg cacaataatg aagctgaccg tgccatgtat tccaccctgt
     1381 tccagcagtc cgaaattatg actccggaag aaatcctgaa ttgccgggaa ccggaaaagg
     1441 aactggatgc gacctcttac gcggcctatc aaacttcctg tggagccctg gtgctttgcc
     1501 tgctgaacca ggaaaacggt ccggaaggga tgaaacagct tctggaccag gctattctgg
     1561 gcaatgacga tcccagaaac ctcatcaaac gccattttcc gcagctcagc ctgaccccca
     1621 cttccctcca taaatggtgg acgcttcagc tttcccgcat ggcgacgcct cccctgacgg
     1681 aaactctcac gattacggaa acggaacgtc atctccagga agcccttacc ctggtcaagt
     1741 atgatccgga tacccgcacc accgtcacat ttcccctgga tgacctggaa caggcccgtt
     1801 ccctgccgga tctgaacagg cagctttcca acgtggctgg cagcctgctc aacctcagca
     1861 ggcgttcctt tccttcatac cggcccgtca ttgtagaata tgccaagctg gtggccctga
     1921 tgcaaacagg gaaagtcaac agccgggaag ccgtccccaa aatcagccag cttcgggaaa
     1981 taagaaacct ttccatgaaa accgcccgcc gcgtccggga ctatatggac tggtatgaaa
     2041 tcaacacgcg ttccggaacc ggcaagac
//
LOCUS       DAOMSU010000350         2054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5786_length_2054_cov_2.618809, whole genome shotgun sequence.
ACCESSION   DAOMSU010000350 DAOMSU010000000
VERSION     DAOMSU010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5786_length_2054_cov_2.618809"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="PK077_10535"
     CDS             complement(<1..1073)
                     /locus_tag="PK077_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRN24776.1"
                     /translation="MRGIFLVSCFLGGSALLAGCREENSPRPATEDAVRITSARQWGE
                     SRSALQKRELDRAAGPALPIPDDLPSRELVGTTRQIHQLIEASGNPAHAESYTETVPA
                     AGASLRMVAIPGGTFLMGSPAEEPHRKPDEGPQHKVSISPFWISETEIPWELYTAFME
                     NGSPRAKDGQLLEEQPDDELWDFVTQPTAPYTAMNLGMGHGYEHGLPAISMSHHAASK
                     FCEWLSAQTGHYYRLPTEAEWEYACRAGSAGAYSYGNGEAPLNQYAWYWNNSNDRYQK
                     TGSKKPNKWGLRDMHGNVAEWVLDSYVPDAYGKRSGLSVKDPLVIVPGAPHIVRGGSW
                     EDDPDGLRSAARRASTPAWNRQDP"
     gene            complement(1097..1843)
                     /gene="fabG"
                     /locus_tag="PK077_10540"
     CDS             complement(1097..1843)
                     /gene="fabG"
                     /locus_tag="PK077_10540"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HRN24777.1"
                     /translation="MQKLAGKTAIVTGAGRGIGNAVARRFASEGARVILISRSPSSCG
                     GAAEEINKEFPDSCKAYPCDVADYAAVQETMTAILKDFPQIDILVNNAGITRDSLMLR
                     MKEEDWDAVLDTNLKSAFNTVKALQRVLMKSPAGRIINMSSVIGLVGNIGQANYAASK
                     AGLIGFTKSLALEFAPRKVTCNAIAPGFISTEMSNAIPDNLKEEIVKKIPLKEQGSVE
                     DIAALTAFLASDDARYITGQVIACDGGMTM"
BASE COUNT          367 a          573 c          663 g          451 t
ORIGIN      
        1 gggtcctggc ggttccaggc gggggtgctg gcacgacgcg ccgcgcttcg caggccgtcc
       61 gggtcgtctt cccatgaacc gccgcgaacg atgtggggag ctccggggac gatgactagg
      121 gggtctttca cagataagcc gctccgtttg ccgtaggcat ccggaacgta ggagtccaaa
      181 acccattccg ccacatttcc gtgcatgtcc cggaggcccc atttattcgg ttttttggag
      241 cctgttttct ggtaacggtc attggagttg ttccagtacc aggcgtactg gttcaggggc
      301 gcttccccgt tgccgtaaga ataggcgccg gcggaaccgg cccggcaggc gtattcccat
      361 tccgcctccg tgggcaggcg gtagtaatgc ccggtctggg cgctcagcca ttcgcagaat
      421 ttgctggcgg cgtgatggga catggagatg gcgggcaggc cgtgttcgta gccatggccc
      481 atgcccaggt tcatggcggt atagggggcg gtgggctgcg tcacgaagtc ccataattca
      541 tcatccggct gttcctccag aagctgaccg tccttggcgc gcggactccc gttttccatg
      601 aaggccgtat aaagttccca ggggatttcc gtttccgaga tccagaaggg ggagatgctc
      661 actttatgct gcggaccttc gtccggtttc cggtgcggct cttccgcggg actccccatg
      721 aggaacgttc ctcccggtat ggcgaccatg cgcagggaag ctcccgccgc gggcaccgtt
      781 tccgtgtagg attcagcatg tgccggattt ccggaagctt caatgagctg atggatttgg
      841 cgtgtcgtgc cgaccagctc ccgggacggc aggtcatccg gaatgggaag ggcaggaccg
      901 gcagcgcggt ccagttcccg tttttgcagc gcggaacggc tttctcccca ctggcgtgcg
      961 ctggtaatgc gcacggcatc ttccgtggcc gggcgggggg aattttcttc ccggcacccg
     1021 gccagcaggg cgcttcctcc cagaaagcag gaaacaagga aaatgccgcg catggaatgg
     1081 agaaaaccgg aatgccctac atcgtcatgc ctccgtcgca ggcaatgacc tgcccggtga
     1141 tgtaacgggc atcgtcagag gcaaggaagg ccgtcagggc ggcgatatct tccacggaac
     1201 cttgttcttt caaaggtatt ttcttgacga tttcttcctt gagattgtcg ggaatggcat
     1261 tggacatttc cgtggagatg aagccgggag caatggcgtt gcacgtgact ttgcgcggag
     1321 cgaattccag ggccagtgat ttggtgaagc cgatgaggcc cgccttggaa gcggcgtaat
     1381 tggcctggcc gatgttgccg acgagaccga tgacggagct catgttgatg atgcgcccgg
     1441 ccggggattt catgagaaca cgctggaggg ccttgaccgt attgaacgcg cttttcaggt
     1501 tggtgtccag aacggcatcc cagtcttcct ccttcatgcg gagcatcagg gagtcccgcg
     1561 tgatgccggc gttgttcacc aggatgtcaa tctgcgggaa gtctttcaaa atggcggtca
     1621 tggtttcctg aacggccgcg taatccgcta cgtcgcaggg gtaggccttg caggaatccg
     1681 gaaattcctt gttgatttct tctgcggccc ccccgcagct ggatggactg cggctgatca
     1741 ggatgaccct ggctccttcc gaagcgaagc ggcgcgcgac ggcattgccg attccgcggc
     1801 ccgcgccggt aacgatggct gttttacctg ctaacttttg catgctgccc acagtaaacc
     1861 cgtttttcac ggttccggca agcttgaaat ggacctggcc gcgcaggacc ccatatgggg
     1921 aaagggcggg ccgggaatga aaaaaggggg agaaacgggg cgagggggag gctactgtcc
     1981 ggcctcttca ttttcccgga ttgtattggg gggcgccccg tcttcatccg gttctaccag
     2041 gccgctgaat tcat
//
LOCUS       DAOMSU010000351         2050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5811_length_2050_cov_3.058145, whole genome shotgun sequence.
ACCESSION   DAOMSU010000351 DAOMSU010000000
VERSION     DAOMSU010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5811_length_2050_cov_3.058145"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1377)
                     /locus_tag="PK077_10545"
     CDS             complement(<1..1377)
                     /locus_tag="PK077_10545"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HRN24778.1"
                     /translation="MMKQYGFSWSAALMAVGVGALAWAGPEAVQNVQKPALSGGTPVV
                     FGFGGEGNQEFMLNGKPFQIRGAEMHPQRIPREYWRHRIRTAKAMGLNTIAFYVFWND
                     HEQPDGSFDFKTGNRDLEGFLKLCQEEGMWVLFRPGPYACGEWDLGGLPHYLLKDPKA
                     KLRTTEDAKFMKAQTRYLEAVARVAEPFLAKNGGPILMTQLENEYGSYQRKDRKYMEW
                     LKAFWSRKGFGPFYTSDGAGEHFLKGVVLPGVAVGLDPGLNDGHWAVANKCNPGVPVF
                     SSETYPGWLRHWGEGNWAPTPGVVNHVRWFMDKGRSFSLFVFHGGTNFGFSAGANNGG
                     PGKYQPDLTSYDYGSPVDEQGRMNEYYAQMRDIILKKLPPEAAVPEPPADIPAMEIPE
                     FTPAVHAGLWENLPKPFRSKFPQPPYFEQWNQNQGIAVYSTAVPSGPPETLEFTNVND
                     YAQVYLDGE"
     gene            1576..>2050
                     /locus_tag="PK077_10550"
     CDS             1576..>2050
                     /locus_tag="PK077_10550"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRN24779.1"
                     /translation="MNMKTIVLLRHGESTWNRENRFTGWTDVDLTELGIQEANHAGDL
                     LKDRGMAFDHAYTSYLKRAVKTLNCVLDRLDQDWLPVSKSWRLNEKHYGMLQGLNKSE
                     TAQKYGDEQVLIWRRSYDVAPPPLAEDDPANPKWDPRYKGVPDSELPRTESLKETI"
BASE COUNT          481 a          600 c          521 g          448 t
ORIGIN      
        1 ctctccatcc agatacacct gggcatagtc attgacattg gtaaattcca gcgtttcagg
       61 cggtcctgac ggaacggccg tgctgtaaac ggcaataccc tggttctggt tccattgttc
      121 aaaataggga ggctgcggga acttggaccg gaaaggcttg ggcaggttct cccaaaggcc
      181 ggcatgcact gcgggcgtga actccggaat ttccatggcc ggaatgtctg cgggaggttc
      241 cggcacagcg gcttcgggag gcaacttttt caaaatgatg tccctcatct gggcataata
      301 ttcattcatc cgcccctgct catccacggg agaaccgtaa tcataactcg tcaggtccgg
      361 ctggtatttt cccggccctc cgttgttggc tccggccgag aatccgaaat tggtacctcc
      421 gtggaaaacg aacaagctga aggaacgccc cttgtccata aaccagcgga cgtggttgac
      481 cactccaggg gtgggagccc aattcccctc cccccagtgc cgcagccagc ccggatacgt
      541 ttccgaggaa aaaacgggaa ctcccggatt gcatttatta gccactgccc aatggccgtc
      601 gttcagcccc ggatccagcc ctacggccac gcctggaagc accacgcctt tcagaaaatg
      661 ttctcccgcg ccgtcggagg tgtaaaaggg gccaaaaccc ttcctgctcc agaacgcctt
      721 cagccattcc atatacttgc ggtctttacg ctggtagctc ccgtattcgt tttcaagctg
      781 ggtcatcaga atgggccccc cgtttttggc taaaaaaggc tccgccacac gagccacggc
      841 ctccagataa cgcgtctgcg ccttcatgaa tttggcgtct tccgtagttc tcaacttggc
      901 cttgggatcc ttcagcaaat aatgaggcag tccacccagg tcccattccc cgcatgcata
      961 ggggccgggg cggaataaaa cccacatgcc ttcctcctgg cataacttga ggaacccttc
     1021 cagatcccga ttgcccgtct taaagtcaaa gctgccgtcc ggctgctcat ggtcattcca
     1081 gaacacataa aatgcaatag tattcagccc catagccttg gcggtcctga tgcggtgcct
     1141 ccagtattcc cggggaatgc gctggggatg catctccgcg ccgcggatct ggaagggttt
     1201 cccgttcagc atgaactcct ggttcccttc ccctccgaac ccgaacacaa cgggggttcc
     1261 tccggaaaga gcgggtttct gtacattctg gacagcctca gggccagccc atgccaatgc
     1321 tcccactccg acggccatca gtgcggcaga ccaactgaaa ccatattgct tcatcatgtt
     1381 gaacagttaa ttcatttgaa gacggttgcc aataacaatt tttttctctc ttctccgaat
     1441 cttcggaata cccttcccct ccgccagaaa tctgacgggg ccggacggaa cggaggcctt
     1501 ccgttccgtg gaatgtgatc atgtcatata tcttcatccc tttgtatgaa aaccggagga
     1561 cgcctgtcta aatttatcaa tatgaaaaca attgttcttc ttcgccatgg agaaagcacg
     1621 tggaaccgtg aaaacagatt caccggatgg acggacgtgg atctgacgga attgggaata
     1681 caggaagcca accatgcggg cgaccttctg aaagacaggg gaatggcctt tgaccatgcc
     1741 tacacctctt atctcaagag ggccgtgaaa acgctcaact gcgtgctgga ccgtttggac
     1801 caggactggc tgcccgtctc caaaagctgg aggctgaatg aaaagcacta cggcatgctc
     1861 cagggcctga ataaaagtga aacggctcaa aaatacggag acgaacaggt actgatctgg
     1921 aggcgcagtt atgacgtggc gcctcctccc ctggcggagg acgatcccgc caaccccaaa
     1981 tgggatcccc gctacaaggg agtgccggac agcgaacttc cgcgaacgga atcactgaaa
     2041 gaaactattg
//
LOCUS       DAOMSU010000352         2048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5830_length_2048_cov_1.474661, whole genome shotgun sequence.
ACCESSION   DAOMSU010000352 DAOMSU010000000
VERSION     DAOMSU010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5830_length_2048_cov_1.474661"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            173..1621
                     /locus_tag="PK077_10555"
     CDS             173..1621
                     /locus_tag="PK077_10555"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRN24780.1"
                     /translation="MDWKTGTCPKKAPVSVNKDAALTKSLGREGYELQVRPGSILIRA
                     ATDSGVFYARRTLDQLGAKGNYPCCDIKDSPAFAIRGFMHDTGRNFRPVETLKADIDE
                     MARLKLSAFHWHLTDYPAWRIQSRKYPVLNDPSKRIKDRDVNDTYTYDQIRDLFRYAR
                     ARHIQIIPEIDMPGHSTYFKNCFGFPMHDPRGIKILEELLEEFCREIPVEMSPYLHIG
                     ADEIHIPNGKQFADRMAAKVKSLGRQPIQWAGNNDLPVSGDSYSQLWNDENSVGLPDP
                     AKQKTPYFDSTAGYVNSFDPGILVRRNFFRQPCGTVKSDGHSLGVIQCLWPDTRVGDK
                     KNIPIQSPQWPAMFAMAERSWKGLPEDGSRFAGKLPEKDTEAYQAFSLFEKRMEALAG
                     NKPFPYWRDSFLEWTVFGPVPKDRQEEVRNSLLAGKSPAGLPPIQARGGNLYFPCRVG
                     RPVLQNQAGKYSLGGNDPLCGQARNHVRHGRI"
BASE COUNT          492 a          579 c          549 g          428 t
ORIGIN      
        1 gagtttttta tttaagtgta gtattcatca catgcgaccc actctctctg ttcttttgct
       61 gacgtcaagc ttaacgcttt gtcctctttc ttcctacgga gattggtctt cacctcactc
      121 cctccagcag gaaaaaaacg cgccggttcc tctcattccc tttccgtccc aggtggactg
      181 gaaaacaggg acatgcccca aaaaagcacc cgtttccgtt aacaaggacg ctgccctgac
      241 caagtctctg ggaagagaag gctatgagct ccaggtccgc cccggaagca tcctgatccg
      301 ggcagccacg gattcgggtg ttttctatgc ccgcagaacg ctggatcagc tgggagctaa
      361 gggaaattac ccgtgctgcg acatcaagga cagcccggcg ttcgccatcc gcggcttcat
      421 gcacgacaca ggccgcaatt tccggcctgt tgaaacgctc aaggcggata ttgacgaaat
      481 ggcgcggctg aagctgagtg ctttccactg gcacctgacg gactatcccg catggcgcat
      541 ccagagcaga aaatatcctg ttttgaatga tccctccaag cggatcaagg accgggatgt
      601 caacgacacc tacacctacg accagatccg ggacctgttc cgctacgcca gggcgcgcca
      661 catccagatc attccggaaa ttgatatgcc agggcacagc acttatttta aaaactgctt
      721 cggcttcccg atgcacgacc ccaggggcat taagattctg gaagaactgc tggaagaatt
      781 ctgccgggaa atccctgtgg aaatgtcccc ttacctgcac attggcgctg atgaaatcca
      841 catccccaac gggaaacagt ttgcggaccg gatggcggcc aaggtcaaat ccctgggacg
      901 ccagcccatc caatgggcag gaaacaatga tctgcccgtt tccggagaca gctactccca
      961 attgtggaat gacgaaaatt ccgtaggcct accggatcca gccaagcaga agacccccta
     1021 tttcgactcc acggcaggct atgtcaattc ctttgatccc ggcattctgg tgcgcaggaa
     1081 tttcttccgc cagccatgcg gaacggtgaa gagcgacggg cactctctgg gagtcatcca
     1141 gtgcctgtgg ccggataccc gggtgggaga caaaaagaat attcccattc agagccccca
     1201 gtggccggcc atgtttgcca tggcggaacg cagctggaag gggttgccgg aggatggttc
     1261 ccgctttgcc gggaaactac cggaaaagga tacggaggcc tatcaggcct tttccctgtt
     1321 cgaaaagcgc atggaagccc tggccggaaa caagcctttc ccctattgga gggattcttt
     1381 tttggaatgg accgtattcg ggcccgttcc gaaggacagg caggaagaag taaggaacag
     1441 cctgctggca ggtaaatctc ccgcagggct gccccccatc caagccaggg gaggcaattt
     1501 gtatttcccg tgcagggtcg gaaggcctgt tctccaaaac caagccggga aatacagcct
     1561 gggcggaaac gaccctttat gcggccaagc ccggaaccat gtacgccatg gtaggatttg
     1621 acgctccggc ccgttctacg cgccgctgtt ccgggattcc gtccgccggg gaatggtctc
     1681 aatgcgacac cagaatatgg gtgaacggaa aagaagtgaa aaatccccag atctgcaagc
     1741 tggcgggaca gcggcgttat gaaaagcaca cctggaattc ccccgccaat gagatacctt
     1801 tgacaatgag gaattctggt gggcgcgtcc tcccgtttcc ttccagataa aggccggaga
     1861 aaataaaatc ctgattgaac agccgtatac gggcagcttc cagtcctggg gcatcagctt
     1921 cattccggtg aaaaaatcgg gagaccgctg gatttccgat ccaggctatt ccgtccgggg
     1981 cggcgcggaa aaataagctg ctacttattg actgaaatga aagggaaacg cctgttcttc
     2041 ctgctgtc
//
LOCUS       DAOMSU010000353         2034 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5885_length_2034_cov_2.945427, whole genome shotgun sequence.
ACCESSION   DAOMSU010000353 DAOMSU010000000
VERSION     DAOMSU010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5885_length_2034_cov_2.945427"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="PK077_10560"
     CDS             complement(<1..614)
                     /locus_tag="PK077_10560"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HRN24781.1"
                     /translation="MFLFIDNYDSFSWNLVQAFYALGRKPVVRANDDPHILELATSPE
                     LEAVCISPGPSHPRNAGLCLEFLKRLPASVPVLGVCLGHQLLGYFAGAEVSRAPYVMH
                     GKSSDIIHDGAGLFAGLPNPMTVGRYHSLVVQSKEDEPNPRFTVTSRGPEGEVMALRY
                     NDRPWVGIQFHPESILTPDGLQLLGNFPDNVVPSGQKEKRISRIL"
     gene            complement(598..>2034)
                     /locus_tag="PK077_10565"
     CDS             complement(598..>2034)
                     /locus_tag="PK077_10565"
                     /inference="COORDINATES: protein motif:HMM:NF012640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HRN24782.1"
                     /translation="FFYFQLTSSSMQTPSMITLQQRSRRLSADLETPISLFLSLTQNK
                     IPGLLLESAEVDGRWGRYSIIACDYLMTVSCVDARLSLSIKDDRLASLKELEGMPYLD
                     GLRSLMQRLELVGDDMRQAPITRALYGYFGYETAALFQPRLAQAIPASSAESCLVLAG
                     TVIVFDHLYNRLTQLSLGEHRDLSHAPLHGAEEPSIGEVSRTPDQAAYMKGVEHIKEL
                     LHDGEAIQVVLSSQASAEFRGDAFMLYRRMRSINPSPYMFFMRLPEVTLFGSSPELMV
                     RCTDGKLQLSPIAGTRRRGRDDEEDAALAADLLKDPKECSEHVMLVDLGRNDLGRVAK
                     PGSVKLERLMEIERFSHVMHMTSRVTAQVNDGLDVLDILGAAFPAGTVSGAPKVRAME
                     ILAEEEPLPRGPYAGCIGWLGLDKNGVHMDSGITIRSMWVKDGRIHWQTGAGIVYDSD
                     PAAEWQECMNKGKIIDVILKGEDHVSVH"
BASE COUNT          447 a          599 c          594 g          394 t
ORIGIN      
        1 agaatgcggc tgatgcgctt ttccttctgc cccgagggca cgacgttatc cgggaagttg
       61 cccagaagct gaagcccgtc cggcgtcagg atggattccg gatgaaactg aatgcccacc
      121 cacggccggt cattgtagcg cagagccatg acttcccctt ccggaccgcg ggaagtaacg
      181 gtaaaacggg ggttcggctc gtcctccttg gactgaacca ccagggaatg gtagcggccc
      241 acggtcatgg gattgggaag tccggcaaac agccccgcgc cgtcatggat gatgtcggag
      301 cttttgccgt gcatgacata aggggcgcgg gaaacctccg ctccggcgaa atagcccagg
      361 agctggtgcc ccaggcacac gcccagcacg ggaacggaag cggggaggcg tttcaggaac
      421 tccaggcaca gcccggcgtt gcgcggatga ctggggcctg gggaaatgca tacggcttcc
      481 agctccgggc tggtcgccag ttccaggata tgcggatcgt cattcgcacg caccacgggc
      541 ttgcgtccca gggcgtaaaa cgcctgcacc agattccacg aaaaagaatc gtaattatca
      601 atgaacagaa acatggtctt ctcctttcaa aataacgtca ataattttgc ccttgttcat
      661 gcactcctgc cattcggctg ccggatcgga atcatatacg attccggctc ccgtctgcca
      721 gtggatgcgc ccatctttca cccacatgct gcggatggtg atgccggaat ccatgtggac
      781 gccgtttttg tccaacccca gccatccgat gcatccggcg tacggtccgc gcggcagagg
      841 ctcttcctcc gccaggattt ccatggcgcg caccttgggg gctccgctaa ccgtcccggc
      901 agggaaagct gcgccgagaa tatccaagac gtccaatccg tcgttcacct gggcggtcac
      961 gcgggaagtc atatgcatca cgtgggaaaa acgttcaatc tccatcaggc gttccagttt
     1021 cacggaaccg ggcttggcga cacgtcccag gtcgttgcgc cccagatcca ccagcatgac
     1081 atgttccgaa cattccttcg gatctttcag gagatcggca gccaaagcgg cgtcttcctc
     1141 atcatccctg cctcgcctgc gcgttccggc aatgggggaa agctgaagct tgccgtccgt
     1201 gcagcgcacc atcagttccg gagaagatcc gaacaaagtc acctccggca ggcgcatgaa
     1261 aaacatgtat ggggaaggat taatgctgcg catgcggcgg tacagcatga aagcgtctcc
     1321 gcggaactcc gcggaagcct gggaggaaag caccacctgg atggcttctc cgtcatgcag
     1381 cagctccttg atatgctcca cccccttcat gtaagccgcc tggtccggcg tgcgggaaac
     1441 ctcccctatg gaaggttcct ccgccccatg gagcggggca tgggacaaat cacggtgttc
     1501 acccaggctg agctgggtaa ggcgattgta caaatggtca aaaacaatca cggtgccggc
     1561 cagcaccagg caggattctg cggaagaagc cggaatggcc tgcgccagcc tgggctggaa
     1621 cagagcggcg gtttcatacc cgaaatagcc gtacagagcc cgcgtgatgg gagcctgcct
     1681 catatcgtcc cccaccagct ccagacgctg catcagagag cgcagtccat ccaggtaagg
     1741 catgccttcc agctccttca gggaagccag acggtcgtct ttgatggaaa gggacagcct
     1801 ggcgtctaca caggaaacgg tcatcagata atcgcaggcg atgatgctgt agcgccccca
     1861 tctgccgtcc acttccgcgc tttccagcag aagtccggga attttgttct gagtcaggct
     1921 cagaaacagg ctgatagggg tctccagatc ggcgctcagg cggcggctgc gctgttggag
     1981 agtgatcatg gatggtgttt gcatggatga tgatgtaagt tgaaaataaa aaaa
//
LOCUS       DAOMSU010000354         2024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5939_length_2024_cov_2.545962, whole genome shotgun sequence.
ACCESSION   DAOMSU010000354 DAOMSU010000000
VERSION     DAOMSU010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5939_length_2024_cov_2.545962"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="PK077_10570"
     CDS             complement(<1..381)
                     /locus_tag="PK077_10570"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HRN24783.1"
                     /translation="MFAGAVVVCVAAEWYIYSVSEGRIVEHSRDVPVRAPVLVLGCSP
                     TFMGSPNGYFHNRMDTASELWKDGKATVFIVSGDNSSHAYNEPEWMKQALVERGVPEE
                     RIVCDFAGLRTLDSVVRMKEIFGVS"
     gene            432..707
                     /locus_tag="PK077_10575"
     CDS             432..707
                     /locus_tag="PK077_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24784.1"
                     /translation="MNHLIFQTGRYPTISYSVFLFFSQRRPVINSFFRTVSTQHSSLC
                     FLHFMTRIATKHHHPCFTHPGKQKLLMENQPANDIAAKQKGPQTASL"
     gene            complement(737..819)
                     /locus_tag="PK077_10580"
     tRNA            complement(737..819)
                     /locus_tag="PK077_10580"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(784..786),aa:Leu,seq:taa)
     gene            complement(889..1350)
                     /locus_tag="PK077_10585"
     CDS             complement(889..1350)
                     /locus_tag="PK077_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRN24785.1"
                     /translation="MDKIRQRLSDLGYAIYDAPAPVGSYVQCVRTGNLLHLSGGISVN
                     GEDKYFGKVGQELTVEEGQAAARAAILNRLAVIVQAVGSLEKVSRIVAVNGFVNAGPD
                     FYDHPKVLNGASDLLVEAFGEIGRHSRTAVGVSALPLNVAVEISMVVEVRD"
     gene            1504..>2024
                     /locus_tag="PK077_10590"
     CDS             1504..>2024
                     /locus_tag="PK077_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24786.1"
                     /translation="MMTDETPSTDPKQQIPDLSGLADFQFGPAWARPGARQERPASYP
                     ERRSGDRQGGDRRPRRDNREGRQPRGERRVDGQSGERRFNREDRTRGSFRNRRDQTPR
                     SAMAEPTEGLRVELRPVDSGLAALHQEVQKHRRTISLLDLAKVVMGSYDRYDLVFMKQ
                     ENGPDLYHCKHGDG"
BASE COUNT          518 a          597 c          460 g          449 t
ORIGIN      
        1 cgaaacgccg aaaatttcct tcatcctcac gacagaatcc aaagtccgca atcccgcaaa
       61 gtcacataca atccgttcct ctggaacgcc acgttccacc agagcctgtt tcatccattc
      121 cggttcattg taagcatgag aactattatc tccggaaacg atgaaaaccg ttgcttttcc
      181 atctttccaa agttccgagg ccgtatccat cctgttatgg aaatatccgt tagggctccc
      241 catgaatgtt ggggagcatc ctaacaccag aacaggagca cgaacgggaa catccctgga
      301 atgctcaaca atcctgccct ctgaaacgga ataaatgtac cattctgcgg caacgcatac
      361 cacaaccgct cccgcaaaca tgcctcctgc tattttcaac gccagcagca ttcccttttt
      421 tccagggcgc catgaaccat ctgattttcc aaaccggaag atacccaaca atttcatact
      481 ccgtatttct atttttcagc cagaggcgcc ctgttataaa ctcttttttc agaactgttt
      541 ccactcaaca ttcttctctc tgctttctgc atttcatgac aagaatagcc acgaaacacc
      601 accatccttg ttttactcat ccgggaaaac aaaaattatt gatggagaat cagccagcaa
      661 atgatattgc agcaaaacaa aaaggcccgc aaacagcgag cctttaattt acataattca
      721 ttgttattta aagaagtacc cccgaccgga ctcgaaccgg tacgtccttg cggacaacag
      781 attttaagtc tgcagcgtct accattccgc cacgagggca ttgaccggac ggcactctac
      841 aggaatgccg ccgggattca agcacgaatc ccttccggtt tttaaatatt aatcacgaac
      901 ttccaccacc atgctgattt ccaccgctac attgagcggc agggcggata cgcctaccgc
      961 cgtacggctg tggcggccaa tttctccaaa ggcttccacc agcaggtcgg atgcgccatt
     1021 caatacttta ggatgatcat aaaaatccgg gccggcattc acaaatccgt tcacggccac
     1081 gattctggat actttttcca gagaccccac cgcctgcaca ataacggcca gacgattcaa
     1141 aatagcggcg cgcgcagcgg cctgaccttc ttccaccgtc agctcctgcc ctactttacc
     1201 aaagtatttg tcctccccgt tcacggagat gccgccggag aggtgaagca ggttgcccgt
     1261 gcgtacgcat tgcacatagg agcctacggg agcgggagca tcgtaaatgg cgtaaccaag
     1321 gtcggaaagt cgttgacgta ttttatccat atagagcatt tgacagaagt tgagttgaaa
     1381 ggatacaccg gaaacagttg catttccaga tgcctttcat gacattttga acaattttcg
     1441 gtctgtttgc acgtaaaatg attgccatcc acctcattta gttgctattg aaacaacccg
     1501 tacatgatga ccgacgaaac tccttccaca gaccccaagc agcagattcc ggacctttcc
     1561 ggcttggccg actttcaatt tggtccggcc tgggcaagac cgggagcccg tcaggaacgg
     1621 cctgcgtcct atccggaacg caggtccggc gacaggcagg gaggcgaccg ccgaccgcgg
     1681 cgcgacaacc gtgaaggccg ccagccccgg ggggaacgtc gtgttgacgg tcaaagcgga
     1741 gaaagaaggt ttaaccggga agaccgcacg cgcggctcct tccgcaaccg ccgcgatcaa
     1801 actccccgct ctgccatggc ggaaccgacg gaaggcctgc gcgtggagct gcgccccgtg
     1861 gattccggcc tggctgccct gcaccaggaa gtacagaaac accgccgcac catttccctg
     1921 ctggatcttg ccaaggtggt catgggttcc tacgaccgtt acgacctggt attcatgaag
     1981 caggaaaacg gcccggatct gtaccattgc aagcatggcg acgg
//
LOCUS       DAOMSU010000355         2016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_5969_length_2016_cov_2.301887, whole genome shotgun sequence.
ACCESSION   DAOMSU010000355 DAOMSU010000000
VERSION     DAOMSU010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5969_length_2016_cov_2.301887"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1651)
                     /locus_tag="PK077_10595"
     CDS             complement(101..1651)
                     /locus_tag="PK077_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24787.1"
                     /translation="MPSPSQDTVACTCPSCGNLFAVKASFLGRQVKCPICTASVTARQ
                     EEKPRPETEEQDAVPCLCHICGNSFAVRAACVGSRVECPVCHSAVTAVKREEALSTFP
                     GRTPEAAEKAPGNPKHTAIPPSAAGVPHTSAYSAPHTPLAPKKKTDKRTISVHGPTTP
                     GPNKSTTKIRKRKEGNAAPAIAAPRCAALAPEEPEYRAAAADTMRSERRPTWHIWLFV
                     AGLVLSLLGVFMFLRGRDMEAENSKLLNISDRFVDHEALFSQEVNKDLLEQLQQRERQ
                     YRLIAHSRREEDRKAESISPHITAAMNELALYCMAESDEERLNYVMNPAAVAPKMAYW
                     ASYGQYKDYLPQEAGRSSKNGDLLQISVLMDDNTIRPAVFLYDRNSGKWKLDWEAWEG
                     YSPMLPAELEAKKPSTPVPVRIILSMSGIYQPPFLEESSAESYRNTSYLNFTLEFPNG
                     ERLNAYVDRYSPLALELTRLLYNGPVRTCVLIRYPEDLPGNRAVIIDQLLHSGWMSDA
                     TRRLLPPQ"
     gene            complement(1664..>2016)
                     /locus_tag="PK077_10600"
     CDS             complement(1664..>2016)
                     /locus_tag="PK077_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24788.1"
                     /translation="HTIFEYLRKGDSSLPFCDLHGEDVSAPVSTFTAQNRILPLPPIL
                     PTRPILQGDDPYHTELNQVPANRNFELLGAGENVPEAQAISADPVSSDHTDTSITLPA
                     PRPITIPVPEFIHL"
BASE COUNT          372 a          502 c          682 g          460 t
ORIGIN      
        1 cgtaaatggt ggaagccagg ttgagttgga gggctttgtc ctgtgtttgc gctagtgctt
       61 tggaactcat ggaaagcggg aaccgattct tgattttcaa ttattggggg ggaagaagcc
      121 tgcgggtggc gtcgctcatc cagccggagt gcagaagctg gtcaataata acggcccggt
      181 tgccgggcag gtcttccgga tagcggatga gcacgcaggt gcgcacgggg ccgttgtaaa
      241 gcagcctggt cagttccagg gcaagcgggg aatacctgtc cacgtacgca ttcaggcgtt
      301 ccccgttggg gaactcaagg gtgaagttga gatatgacgt gttgcggtag ctttccgcgg
      361 aggattcctc caggaaaggg ggctgataaa tgccggacat gcttaggatg attctgacgg
      421 gaacgggggt ggagggcttt ttggcttcca gctccgccgg aagcatgggg gaatagccct
      481 cccaggcttc ccagtccagc ttccattttc ctgaattccg gtcatacagg aaaaccgcag
      541 ggcggatagt gttgtcgtcc atcagcacgg agatctgaag caggtccccg tttttggagc
      601 ttctgcccgc ttcctggggc aggtagtcct tgtattggcc gtaggaggcc caataggcca
      661 ttttgggagc tacggctgcg ggattcatta cgtagttgag cctctcttca tccgattccg
      721 ccatgcagta gagggccagt tcattcatgg ccgccgtaat atggggggaa atggattccg
      781 ccttccggtc ttcctccctg cggctgtggg ctatgagccg gtattgccgc tccctctgct
      841 gcaattgctc cagcaggtct ttattcactt cctgtgaaaa gagcgcttca tgatccacga
      901 accggtctga gatattcaga agtttggagt tttccgcttc catatccctc ccgcgcagga
      961 acatgaacac gcccagaagg gaaagaacca gtcccgcgac aaaaagccat atatgccagg
     1021 tgggccggcg ttcactgcgc atggtatccg cggcggcagc gcggtattcc ggctcttccg
     1081 gggccagggc ggcgcagcgt ggcgcggcaa tggccggagc ggcgtttccc tctttccttt
     1141 tacggatttt tgtcgtggat ttattggggc ccggagtggt gggcccgtga acggaaatgg
     1201 tccgtttgtc cgtctttttc tttggtgcga gcggtgtatg cggagcggag tacgcggaag
     1261 tgtgcggaac accggcggca gatggcggaa tagccgtgtg tttcggattt ccgggcgctt
     1321 tctctgcagc ttcaggcgtc cggcccggga atgttgaaag cgcttcttcc cgtttgacgg
     1381 ccgtgacagc ggagtggcag acggggcatt ccacacggct gcctacgcag gcggccctga
     1441 cggcaaagga attgccgcaa atatggcaga ggcaggggac ggcgtcctgt tcttccgttt
     1501 ccggcctggg cttttcttcc tgcctggctg tgactgaagc ggtgcagatg gggcatttta
     1561 cctggcgtcc cagaaaagac gccttcacgg cgaagaggtt gccgcatgac gggcatgtgc
     1621 aggctaccgt gtcctgggat ggggaaggca tgaggggaaa aagttacagg tggatgaatt
     1681 ccggcacggg aatggtgatg ggcctgggcg ccggcagcgt aatgcttgta tccgtatggt
     1741 ctgaagaaac gggatctgcg gaaatggctt gcgcttccgg cacgttttcg ccagccccca
     1801 gcagttcaaa gttgcggttg gccgggacct ggttcagctc cgtatggtag ggatcgtccc
     1861 cctggaggat gggcctggtg ggcaggatgg gcggcagggg aagaatgcgg ttctgggccg
     1921 tgaaggtgga gacgggtgcg gagacgtcct ccccgtggag gtcgcagaaa ggcaggctgg
     1981 aatccccctt gcggaggtat tcgaatatgg tgtgtc
//
LOCUS       DAOMSU010000356         2007 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6011_length_2007_cov_2.087602, whole genome shotgun sequence.
ACCESSION   DAOMSU010000356 DAOMSU010000000
VERSION     DAOMSU010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6011_length_2007_cov_2.087602"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            457..>1422
                     /locus_tag="PK077_10605"
     CDS             457..>1422
                     /locus_tag="PK077_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24789.1"
                     /translation="MKRAFCVIAGACLLGWAPADETGMNNVLEDWRMRQSDWESALKT
                     AESGEKRAELMKNRPDAVPVARELWRLVGRDLQKPETQKSCLLPAVVWFLDHPQAVAQ
                     AFPNGDMARKIVVFCLDSLENTLFREQGAGKAAYALSNSRDLRCRVILEQIKDYNQFP
                     EDQGLASLGLAMVMKETTGMLQDDVRLVAARGKLLKDAIIKCYDSSFGPIPVQNIVKE
                     ELYEIRNLNIGQTGPEISLPSTAAAGKVTLPSGNKPVLVVFWDPQDMRSVQFLQKAAS
                     LRKEFPGLTVMPVAPGKRENIEKALLNLKMDMPSLVDEKAAAFKDY"
     assembly_gap    1423..1448
                     /estimated_length=26
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1449..1735
                     /locus_tag="PK077_10610"
     CDS             <1449..1735
                     /locus_tag="PK077_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24790.1"
                     /translation="KILMRGTPDMLFDANLYAVMGKLEGKKKEKSDGKKSPSSPASSK
                     PVSPPARINSPDTRVPSVSRPDRPRPAFPSAPSGAEDHSLAAPPLRPMPE"
     gene            1744..>2007
                     /locus_tag="PK077_10615"
     CDS             1744..>2007
                     /locus_tag="PK077_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRN24791.1"
                     /translation="MEGILLRKAAEDVARVLSGAGHTVYFVGGCVRDRLLGYSVKDID
                     IATSARPDEVLRLFPDAWEVGAAFGVMLVRREGFSFEVATFRRD"
BASE COUNT          441 a          478 c          601 g          461 t
ORIGIN      
        1 gtttaattcg tgaaggtatc ctgaatgaac agggatgttc gataagggag gaacgagagc
       61 ttcccttgtc accaggaata accctgattt cataggtagt tagttggagc ggctacggga
      121 gaaattccgt agccgctttt ttgtcttccc gggccttgtg tcatacaaaa gaaataatgt
      181 ttgaacatat ttgccgtgcg tttgtctcac ccataacggt tatgatccgg atgcagtgaa
      241 agagagagcg cttcatccac ttaaggactc gaccgtcttc ataggtagtt agttgaacag
      301 cagccccggc gaagggattc gccggggttt gttcatttcc ggaagatgta ttccgcgctg
      361 taaacagctg gaagggaggg gaaaagaaaa aataggctgc gttttgccac ggggtttgat
      421 tgacgtgtgg gatgcgtcag ggcatagtgg cagggtatga agagagcgtt ttgcgtgata
      481 gccggtgcat gcctgcttgg atgggctccg gcggacgaga cgggcatgaa caatgtgctg
      541 gaggattggc ggatgaggca gtccgactgg gaatccgcat tgaaaacggc tgaaagcggg
      601 gaaaaaaggg cggaattgat gaaaaaccgt ccagatgccg ttccggtggc tcgggaatta
      661 tggaggctgg tagggcgcga ccttcagaaa cccgagactc aaaaatcctg ccttcttccg
      721 gctgtcgtct ggtttctgga tcatccccag gctgtagcgc aggcgttccc caatggagac
      781 atggccagga aaattgtcgt gttctgcctg gattccctgg aaaatacgct gttccgggaa
      841 cagggggcgg gaaaggcggc gtatgccctg agcaattccc gtgacctgcg ctgccgcgtt
      901 attctggaac agatcaagga ttacaaccag ttccctgagg accagggcct ggcttccctg
      961 ggcctggcca tggtgatgaa ggaaacgaca gggatgcttc aggatgacgt caggctggtg
     1021 gcggcccgcg gaaaattgct gaaagacgcc attatcaaat gctatgattc cagtttcggc
     1081 cctattccgg ttcaaaatat tgtgaaggag gaactgtatg aaatacgcaa tctcaatatc
     1141 gggcagaccg ggcctgaaat cagccttccc tctaccgcgg cggccggaaa agtgacgctc
     1201 ccctccggga ataagcccgt gctggtggtt ttttgggatc cgcaggatat gcgttccgtt
     1261 cagtttttgc aaaaggccgc gtctctccga aaagaatttc ccggtttgac ggtgatgccg
     1321 gtggctcccg gaaagagaga gaacatagaa aaggccctat tgaacttgaa aatggatatg
     1381 ccctccctgg tggatgaaaa agcggcggcg tttaaggatt acnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnngg aaaatcctga tgcgcggcac gccggatatg ctgtttgacg ccaacctgta
     1501 cgcggtcatg ggcaaacttg agggaaaaaa gaaagaaaag tcggatggga aaaagtctcc
     1561 ctcctcccct gcttcctcca agcctgtttc tccgccggcc aggattaatt ccccggatac
     1621 gcgcgttccc tctgtttccc ggcctgatag gccgcggccc gcgttccctt ccgccccctc
     1681 cggtgcggag gaccattctc ttgccgctcc tcctttgagg cccatgcctg aatgatccgt
     1741 ttcatggagg ggattctgtt gagaaaggcc gcggaggacg tggcccgggt tctttccgga
     1801 gcaggacaca ccgtatattt tgtgggcggc tgtgttcggg acagattgct gggatattcc
     1861 gtgaaggata tcgatattgc tacttccgcc cgcccggatg aagtgttgcg cctgtttccg
     1921 gatgcctggg aagtgggcgc cgccttcggc gtgatgctgg tacgccgcga aggtttttcc
     1981 tttgaggtgg cgaccttccg cagagac
//
LOCUS       DAOMSU010000357         2005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6022_length_2005_cov_2.515897, whole genome shotgun sequence.
ACCESSION   DAOMSU010000357 DAOMSU010000000
VERSION     DAOMSU010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6022_length_2005_cov_2.515897"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="PK077_10620"
     CDS             complement(<1..763)
                     /locus_tag="PK077_10620"
                     /inference="COORDINATES: protein motif:HMM:NF024882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRN24792.1"
                     /translation="MALDMQERHAFEHEEGRSPVWIGMAWGAVSLLGGVALLAAMLTF
                     DVREIGWSYLNGSGLVIPGTSNVLGTAGLYGAGIFYWMLGGMAWMLVILLEWWGLYRL
                     LHHGRLPRSVVYGGLFLLLFGCLFLTAGHVPGNEWVARHQIQGAGGLAGHLLGTGLFL
                     PLAGRSAVLAFSGLGYLLALVYAVGMRPRPFCRAMLREWRAWRMNRKEKKLARQTALL
                     AREAARVRASMEDAALSAPRPVRKGRASVPRLQRTG"
     gene            complement(1132..1410)
                     /locus_tag="PK077_10625"
     CDS             complement(1132..1410)
                     /locus_tag="PK077_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24793.1"
                     /translation="MKESNPCVSKEVPEETQSPEAAEEAEPRFAEEDAVARVATNPEP
                     ESVTETVLAGVSGSRELLKSNNTVAALAESADTTANMEIEAILLINSR"
     gene            1409..1849
                     /locus_tag="PK077_10630"
     CDS             1409..1849
                     /locus_tag="PK077_10630"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN24794.1"
                     /translation="MENQISFGNSWQTFISGPKGASLSLTISGLVAGASYQIALVTGC
                     PFEGAGAWNSLVTSNTYDSAAPSIGANSQTIQARTPTGYTISDVIANEDGEIILTVNT
                     PGSSHTPTFNALAISGETASVPEPATASLSLLGLVALMARRRRA"
BASE COUNT          476 a          599 c          444 g          486 t
ORIGIN      
        1 ccccggtgcg ctgcagacgg ggaacggaag cccggccctt tctaaccggc ctgggagcgg
       61 acaaagcggc atcttccatg ctggccctca cgcgggcagc ttccctggca agcagagcgg
      121 tctgtcgcgc cagttttttt tccttcctgt tcatacgcca ggcacgccat tcccgcagca
      181 tggcccggca aaacggacgg ggacgcatcc cgactgcata aaccaaggcc agcaaatagc
      241 ccagcccgga aaaagccagc acagcggatc tcccggccaa cggaagaaac agaccagttc
      301 ccagcaaatg gcccgccagc cccccggcgc cctgaatctg gtggcgcgcc acccattcat
      361 tccccggcac atgcccggcc gtcagaaaca ggcatccgaa caacaacagg aacaaaccgc
      421 cgtaaaccac gcttcggggc agccttccat ggtggagcag gcggtaaagc ccccaccact
      481 ccaacaggat aaccagcatc caggccatgc ctcccagcat ccaataaaaa attcctgcac
      541 catataatcc cgccgttccc agcacattgg aggtacccgg tataaccagc ccggagccat
      601 ttaaataact ccagcctatt tcccgcacat caaatgtcag catagcagcc agaagagcca
      661 ccccccccaa caaagacacg gcaccccatg ccattccaat ccaaacaggg gaacgcccct
      721 cttcatgctc aaatgcatga cgttcctgca tatccaatgc catacctgtt tctattctag
      781 caattcttcg ccccaaactc aaggctaaac agggaagaaa aagaattgcc cgaatccgct
      841 tccctcaaaa caggaattgc cggcttacgg accggaaata cggtttttaa acacttttgc
      901 cactctggaa gagtatcgcc aggaattccc ttttttacct gagaaaagac cgcccctgca
      961 ccgcaaatca ataaaaccga atcaatttca atgattattt ttcatatcca tttgattatt
     1021 tattattagc ctaaatatct attaaaaaaa atgtgccgtt cgacattttt tcatgagtta
     1081 tgaaacatcc agcgaatttc aaatccgtta cttactgaaa tcagttgctg tttacctgct
     1141 atttatgaga agaatcgcct ctatttccat gttggctgtg gtatccgcgc tttccgcgag
     1201 tgctgctact gtattatttg attttaacag ttctctgctt ccggaaactc ctgccaagac
     1261 tgtttctgta accgattccg gttcaggatt tgttgcaacc ctggctacgg catcctcttc
     1321 cgcgaatctg ggttctgctt cttccgccgc ttctggggac tgggtttcct ccggaacttc
     1381 ttttgataca cagggattgg attctttcat ggaaaaccag atttctttcg gaaactcttg
     1441 gcagaccttt atctccggcc ccaaaggagc ttctctttcc ctgactattt ccggtttggt
     1501 ggctggtgct tcctaccaga ttgcccttgt taccgggtgt ccttttgagg gggccggtgc
     1561 ctggaattcc ttggtgactt ccaataccta cgacagcgcg gctccttcca taggtgcgaa
     1621 ctctcagaca attcaggccc ggactcccac ggggtacacc attagtgacg ttattgccaa
     1681 tgaagatggc gaaattattc tgacggtgaa tactcctggt tcctcccaca cgcctacgtt
     1741 caatgctctg gctatttccg gagaaacagc cagcgttccg gaaccggcca cggcgtccct
     1801 gagcctgctg gggctggtgg ccctgatggc tcgccgccga agagcttgag aatctctttt
     1861 ccggcagtta aaaatgttgt tgcctgcaag gttctgcctt gcaggcaatt ttttgtaatc
     1921 atccgatttg agaaaactgg gccgtccgaa taaccgtttt tccctggcgg aactcttgct
     1981 ccggaggaaa tgaggctcct gcttc
//
LOCUS       DAOMSU010000358         2001 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6039_length_2001_cov_2.150051, whole genome shotgun sequence.
ACCESSION   DAOMSU010000358 DAOMSU010000000
VERSION     DAOMSU010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6039_length_2001_cov_2.150051"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1458
                     /gene="purH"
                     /locus_tag="PK077_10635"
     CDS             <1..1458
                     /gene="purH"
                     /locus_tag="PK077_10635"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HRN24795.1"
                     /translation="ISTGGTAAFLKGLGLPVIEISDYTGEPELFEGRLKTLHPMVHGG
                     LLHRRDNEEHVRQAKENGIKPIDLVCVNLYPFEETVARPGVTLEEAIEKIDIGGPSML
                     RSAAKNYASVTVVSDPADYARILDEMQTHKGDTTLKTRENLAVKVFMRTSNYDNAITN
                     YLGHQSAESTKGSFCICAPLYQELRYGDNPHQEASLYGSFGDIFHQLQGKELSYTNVL
                     DIEGAAELITQFRRPTVGILKHTNPCGVGQDDEDLRNAWQKAFETDTQAPFGGVIVVN
                     RPMTEGLARVLSAIFTDVIIAPEYDAEARAILQKKKNCRIIRMNTEAWMQARREPIIR
                     SAPGGFMTMKRDTDVMGLDNLEAKVVTKRPPTEEELTAMRFNWRVVKQVHSNAIVFGG
                     TDRTLGIGAGQMSRVDSARIAVWKAGQAGLDLKGSVVASDAMFPFADGLQVAIDAGAT
                     ACIQPGGSIRDEEVIAAADAAGIAMVFTGHRHFLH"
     gene            1498..>2001
                     /locus_tag="PK077_10640"
     CDS             1498..>2001
                     /locus_tag="PK077_10640"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HRN24796.1"
                     /translation="MLLGVNIDHIATLRQARYATMLDSFNVEPSVLDAAYAAQRGGAD
                     SITLHVRGDRRHMQDADALSVRESVALPLNLEMGNTPEMVDFALRLKPDYICMVPEKR
                     EEITTEGGLDAVFHEKDLAPTMARMADNGIQVSLFIDPEVPQVEAAARLGAPMIELHT
                     GCFANHSG"
BASE COUNT          425 a          584 c          599 g          393 t
ORIGIN      
        1 atttccactg gcgggaccgc cgccttcctg aaaggcctgg gccttccggt gattgaaatt
       61 tccgactata ccggagagcc ggagttgttt gaagggcgcc tgaagacgct gcatcccatg
      121 gtgcacgggg gcctgctgca ccgccgcgac aatgaggagc atgtgcgcca ggctaaggaa
      181 aacggcatca agcccatcga cctggtctgc gtgaatctgt atccttttga agaaaccgtc
      241 gccaggcccg gagtcacgct ggaggaagct attgagaaga tagatatcgg cgggccgtcc
      301 atgctccgtt ccgccgccaa gaattacgct tccgtcacgg tggtttcgga tcctgcggat
      361 tacgcgcgca ttctggatga aatgcagacc cacaaggggg atacgaccct gaagacccgt
      421 gaaaacctgg cggtgaaggt gtttatgcgc acctccaatt acgataacgc catcactaat
      481 tatcttggcc accagagcgc ggaaagcacc aagggcagct tctgcatctg cgctcccctg
      541 tatcaggaat tgcgttacgg ggacaatccc caccaggaag ccagcctgta cggcagcttc
      601 ggggatattt tccaccagct tcagggcaag gagctttcct atacgaacgt gctggacatt
      661 gaaggggcag ccgagctgat tacccagttc cgccgcccga cggtgggcat tttgaagcac
      721 acgaacccct gcggcgtggg ccaggacgac gaagacctgc gcaacgcatg gcagaaggct
      781 tttgaaacgg atacgcaggc ccccttcggc ggcgtgatcg tggtcaaccg cccgatgacg
      841 gaaggcctgg cccgtgtgtt gagcgccatt ttcacggatg tcatcatcgc tccggagtat
      901 gatgcggaag cccgcgccat tctccagaaa aagaaaaatt gccgcatcat ccgcatgaac
      961 acggaagcct ggatgcaggc tcgccgcgaa cccatcatcc gttccgcgcc cggcggattc
     1021 atgaccatga agcgggatac ggacgtgatg gggctggaca atctggaggc caaggtggtg
     1081 accaagcgcc ccccgaccga ggaagaattg accgccatgc gtttcaactg gcgcgtcgtg
     1141 aagcaggtcc attccaacgc catcgttttt ggcggtacgg accgcacgct tggcatcggc
     1201 gccgggcaga tgagccgtgt ggattccgcc cgcattgccg tctggaaggc cggacaggcc
     1261 ggactggatc tgaagggcag cgttgtagcg tctgacgcca tgttcccgtt tgcggacggc
     1321 ctccaggtcg ccattgacgc cggagccacc gcctgcatcc agcccggagg ctccattcgc
     1381 gacgaggaag tgattgccgc cgctgacgcc gcgggaattg ccatggtatt tacgggacac
     1441 cgccatttcc tccattaatt tgaatgttcg tccccgcccc tttgtcttaa aaccgatatg
     1501 ctgctaggtg taaatataga ccacatcgcc acattgaggc aggcccgcta tgcgaccatg
     1561 ctggattcct tcaatgtgga gccgagtgtt ttggacgccg cttatgcggc gcaaaggggc
     1621 ggggcggatt ccatcaccct ccatgtccgg ggtgaccgcc gccacatgca ggatgcggat
     1681 gccctgagcg tccgggagag cgtggctctt cccctgaacc tggaaatggg aaataccccg
     1741 gaaatggtgg attttgcctt gcggctgaaa ccggactata tctgcatggt tccggagaaa
     1801 cgcgaggaaa tcactacgga aggcggcctg gacgccgttt ttcatgaaaa ggatctggct
     1861 ccaaccatgg caagaatggc cgacaacggc attcaggtga gcctgttcat tgatccggaa
     1921 gtgccccagg tggaggctgc cgcccgcctg ggcgccccca tgattgagct gcataccgga
     1981 tgttttgcca accattccgg c
//
LOCUS       DAOMSU010000359         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6060_length_1996_cov_1.996909, whole genome shotgun sequence.
ACCESSION   DAOMSU010000359 DAOMSU010000000
VERSION     DAOMSU010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6060_length_1996_cov_1.996909"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    560..569
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(910..1746)
                     /locus_tag="PK077_10645"
     CDS             complement(910..1746)
                     /locus_tag="PK077_10645"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRN24797.1"
                     /translation="MNLLEIIILGIVEGLTEYLPVSSTGHLLLTESLLNMSQSKEALD
                     ALAVCIQGGAILAVLSLYFPRIKAMAQGVRGNNPAGLKLLVNLILAFLPAAVVGLCCA
                     SLIKEYLFNTYTVAYSWIVGGGVILAYCAWRARGGRSNEGDSGASIESLTPAKALTVG
                     ALQCVAMIPGTSRSLMTMLGGMFVGLSVEAAVEFSFLLGLITLGAATVHDAYKDGALM
                     LESFGWEALAVGTAVSWLSAWLAVKWMVSYLQRHSFSVFGWYRIAIGIVTLVLLSMGL
                     VH"
     gene            complement(1771..>1996)
                     /locus_tag="PK077_10650"
     CDS             complement(1771..>1996)
                     /locus_tag="PK077_10650"
                     /inference="COORDINATES: protein motif:HMM:NF013097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRN24798.1"
                     /translation="TRRYAIVAIAVLAAVITPTPDVATMMLMAVPMYALYEICIILAW
                     MHERKEAARAREEIARFEEDFNNNNSPYNL"
BASE COUNT          425 a          678 c          494 g          389 t
ORIGIN      
        1 caacctgatt cgtccgaatt attatatccc cacgtggggg ccggctccct attaccgcgg
       61 gcattatgtc cgtccccatc attgccggcc catggcaaga ccgccgatga aaccccagtt
      121 taggcccgac cacttccgtc cgggaaacaa caggccggcc cctcagcccg gacggcccaa
      181 tcaacggccg gactttggaa aacccggcaa tttacagcgg ccgccagtac atcatccggg
      241 aatcaaccag cccgaacggc cttctcgccc ttcctccaat aatccttccc gtcccggcgg
      301 cacgaatcgc cccgtcatca accgtccgga aggcaatcgt ccctccagac cggatttcgg
      361 acgtcccagc cgcccttcca ccctgccgtc ttcaaggcct tcctccccca atccaggcca
      421 ctccggcatc aaccgccccg gcaggccctc tcccgtttcc cggccttctt ccccgcccca
      481 ttccgccagc gcacccggca ggccttcttc ccctcattca tctgcgccat cccgtccatc
      541 cccttcaccg aaaccctccn nnnnnnnnna tggtgcgtca aagcccctgt aaaaggaaag
      601 cccctgtcat gcccgtcaat ctcctgagcc ctcgcaccca agatctgctc gccatcccgg
      661 gcatcacctt caccgaaacc ctccaatccc gggaattccc acggaacgca caggagataa
      721 gttttcctga aacgctattc atcgcagggc tgttttgttc cggggatgca aaacggccct
      781 gtgtcatggc tttgtccagg cagcggccaa ccttctccgg gattgttcaa aacccgcttc
      841 ccgctgcggg agagaaccgc cttgccggag aaaaacgttt tccggtgctt gaatgccgga
      901 atccgttttt caatgcacca gccccatgga caggagaacc agcgtcacga taccaatggc
      961 gatgcggtac catccgaata cggaaaaact gtgcctttgc agataggata ccatccattt
     1021 tacagccaac catgcggaaa gccagctgac cgccgtcccc acggcaagag cttcccaccc
     1081 gaaggattcc agcatcagag ctccgtcctt atacgcatca tggaccgtgg ccgcgcccag
     1141 ggtaatcaat cccagcaaaa agctgaactc cacggccgct tccacgctca accccacgaa
     1201 catgcctccc agcatggtca tcaggctccg gctggtgccg gggatcatgg ccacgcactg
     1261 gagtgcgccc accgtcagcg ccttggccgg cgtcaggctt tcaatggagg cgccggagtc
     1321 cccttcattg gaacggcccc ccctggcgcg ccaggcgcaa tacgccagaa taacgcctcc
     1381 ccctacgatc catgaataag ccaccgtata tgtgttgaac agatattctt taatcagtga
     1441 agcgcagcac aggcccacca cggcggcggg aaggaaggcc agaatgaggt tgaccagcag
     1501 tttcagcccg gcgggattat tgccgcgcac gccctgcgcc atggccttta tccgcggaaa
     1561 atacaggctc agcaccgcta ggatggctcc cccctggatg cagacggcca gggcatccag
     1621 agcttccttg ctctgggaca tgttcagcag ggattccgtc aggagcaggt ggcccgtgga
     1681 gctgaccggc agatattcgg tcaggccttc cacaatgccc aggataataa tttccagaag
     1741 gttcatgata ttggcgtggg ttggatgtat ttacaggttg tagggagaat tgttgttgtt
     1801 gaaatcttct tcaaaccggg ctatttcctc ccgggcgcgc gcggcttcct tgcgttcatg
     1861 catccaggcc agaatgatgc atatttcata cagcgcatac atgggaacgg ccatcagcat
     1921 catggtagct acatccggcg taggggtgat gaccgcagcc aggacggcga tagccacaat
     1981 ggcatagcgt ctcgtg
//
LOCUS       DAOMSU010000360         1982 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6120_length_1982_cov_2.951738, whole genome shotgun sequence.
ACCESSION   DAOMSU010000360 DAOMSU010000000
VERSION     DAOMSU010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6120_length_1982_cov_2.951738"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="PK077_10655"
     CDS             <1..610
                     /locus_tag="PK077_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24799.1"
                     /translation="TLEFTNVNDYAQVYLDGELVGTLDRRLGQKSVKLPERRKPGTLE
                     VLVEAMGHINFHISMESDRKGVYGPVKLGTRELKNWTVRALPLKADSIVRAPKGKGPS
                     QKREGAHFRAVVNIEEPQDTFLDMSRYVKGYVWVNGINVGRYWNVGPQLRLYVPAPFL
                     KKGENVIDILDLHEKEPKPVRGMKERNKEPGKINTKNLDNQW"
     gene            complement(982..1635)
                     /locus_tag="PK077_10660"
     CDS             complement(982..1635)
                     /locus_tag="PK077_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRN24800.1"
                     /translation="MNTFSHLSPFLAVISGSLFSGTALCNDIVLPPPDKKGGKPLMQV
                     LHERHSTRSFADKPIPVEILSSLLWAAQGVNRDDPDYRTAPSSRNSNEIEIYVVLPQG
                     TYLYRPETHRLEKVVQGDMRAATGTQEFAATAPLNLVYVVDQSKQPGDFDARRKLVTA
                     CTDAGFIGENVYLFCASEGLGTVFRAMINANYIQQCLKLPVLKKVLYAQSVGYPSKS"
BASE COUNT          476 a          471 c          575 g          460 t
ORIGIN      
        1 aacgctggaa tttaccaatg tcaatgacta tgcccaggtg tatctggatg gagagttggt
       61 cggcacgctg gatcggcggc tggggcagaa gagcgtgaaa ctgccggagc gcaggaagcc
      121 ggggacgctg gaagttctgg tagaggccat ggggcatatt aattttcata tcagcatgga
      181 gagtgaccgc aagggagttt acggtcctgt gaagctggga acgcgggagt taaagaactg
      241 gacggtgagg gcgcttcccc tgaaagctga ttccattgtg cgggctccca aaggaaaggg
      301 gccttcccag aaacgggaag gggcacattt ccgggccgtt gtaaatattg aagagcctca
      361 ggacacgttt ctggatatgt cccgctatgt caaggggtat gtatgggtga acggaatcaa
      421 cgtggggcgc tattggaatg tgggacctca gttaaggctg tatgtcccgg ccccattcct
      481 gaaaaaaggg gagaatgtga ttgatattct ggacctgcac gaaaaggagc ccaagcctgt
      541 ccgcggcatg aaggaacgca acaaggaacc cggaaagata aataccaaaa acctggacaa
      601 ccagtggtaa tgctggtgcg ttttccgctt gcggttccgg attttccatg aatttgaact
      661 atgggaaaga ccgcaagcgg ggagtagatt tcctggcgcc gcagtttccc gggaattccg
      721 gtttgaacat ttctccagcc aatgccgcag ggcggagtgg cggagccggg cttgcccgaa
      781 acgttcatcc aacagttcgc caaacgcttt ttctattgtt gccgggctgt ccggaagagt
      841 gcgctcctga ctggcccggc aatggttcct ggcgttttta tttccatggg aacggcggtt
      901 cccatgacgg ttttccgcag ggcatgagga agagccgccg atggaacggc gagtctcaca
      961 gcgttccggc ttatggtttt ttcaggattt tgacggatag ccgacactct gggcgtacag
     1021 gacttttttg aggacgggga gcttcaggca ctgctggata taattggcgt taatcatggc
     1081 gcggaacacg gtgccgagcc cttcggaagc acagaaaagg taaacgtttt ctccaataaa
     1141 ccccgcatcc gtgcaggccg tgaccagctt tctcctggca tcaaagtctc ccggctgttt
     1201 ggactggtct actacataga ccaggtttaa cggcgcggta gccgcgaatt cctgtgttcc
     1261 cgtagcggcc cgcatgtcgc cctgaaccac tttctccagc cggtgggttt ccggcctgta
     1321 aagataggtg ccctgcggca ggacgacata gatttctatt tcattggaat tccgggaaga
     1381 cggcgccgtg cggtagtcgg gatcgtcccg gttgacgcct tgcgccgccc agagcaggga
     1441 ggacaggatt tccaccggga taggtttgtc tgcaaaggac ctggtggaat gccgttcatg
     1501 aagaacctgc atgaggggct ttcctccctt tttatcaggc ggaggcagca cgatgtcgtt
     1561 acagagagcc gttccggaga agaggcttcc cgaaatgacg gccagaaacg gactgagatg
     1621 agagaaagtg ttcataggca acgggaaaaa catatgtcag ccaggaataa tcagcaggat
     1681 acttgtgttt ttctcatgtt tttggaaaca tgaagaggcc tgatactata aaatggctat
     1741 gaaaatacaa ggaaaaatac tttggtttga ttcttaacgg gttccgggaa aacgaacctt
     1801 cagcaaaaga ccattgtttc aagttgacct cagaaaggta ttgcgctttt atgttacacg
     1861 gaacagggat aaaaccgtct cattcccctg cccaatttgc ttcaacgcgt tcaagctgca
     1921 tccccatgaa tcctgtcaat cctctgcgtc ccggttttcc caggcataaa aaatcctctt
     1981 cc
//
LOCUS       DAOMSU010000361         1980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6125_length_1980_cov_3.108052, whole genome shotgun sequence.
ACCESSION   DAOMSU010000361 DAOMSU010000000
VERSION     DAOMSU010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6125_length_1980_cov_3.108052"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="PK077_10665"
     CDS             complement(<1..720)
                     /locus_tag="PK077_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRN24801.1"
                     /translation="MTATIPKGFHPEPFSYHQELELDIDALSNAGDGIGRVDGWVVFV
                     PFALPGDRVKARVWRNDRNYSSADLVEVINPSPDRVEPGCRLFGTCGGCQYQHFSYDR
                     QLLWKTRQVADLLRLQAGLELPVNPAVASPRQYHYRSKITPHFDKPKEGGKPAIGFLK
                     AGSRRDVVDVPQCPIAMECINEALPLARKSVYQAAARFKRGATILLRASEGTVITNNN
                     AVACERVGDLEFHFLAGDFFQN"
     gene            complement(736..1059)
                     /locus_tag="PK077_10670"
     CDS             complement(736..1059)
                     /locus_tag="PK077_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24802.1"
                     /translation="MRKLLIRSCLIAGLAWTSSCASNKETAPQDPAPVPAVQPEQTAS
                     ANQPAPWAPAPAQQEILPEDENEESAPGPAVLPGDFRPGLRTPRLPETLLYDLDGKLI
                     TPSAP"
     gene            1204..1776
                     /locus_tag="PK077_10675"
     CDS             1204..1776
                     /locus_tag="PK077_10675"
                     /inference="COORDINATES: protein motif:HMM:NF025268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRN24803.1"
                     /translation="MRPFLFLCCSAAVSGLFAPQAGIAQQRPPIFGFANKDRIPEVNK
                     SGSPVFDKLRNEAMKKKQPLVIYLTGSSWCSQCNIFTREYIKKTNFKNAAGKKFIFWL
                     VDTKQVPGNSPGTFQFKMVPEEAAKIVGCVDSPRAPYVVLGPPAVFIIDPVSGKLIKS
                     LFSKSEVDKYGKPLAGIIEETWVEFSRKPK"
BASE COUNT          443 a          492 c          582 g          463 t
ORIGIN      
        1 attctggaaa aagtctcccg ccagaaaatg gaattccaaa tcgcccaccc gttcgcaggc
       61 gacggcattg ttattggtga tgacggttcc ttccgaggcc cggagcagaa tggtggctcc
      121 ccgtttgaac cgggcggcgg cctggtaaac gcttttgcgg gccaggggca gggcttcatt
      181 gatgcactcc atggcaatcg ggcattgcgg cacatccacc acatcccttc tggagcctgc
      241 tttcaggaaa ccgatggccg gcttgcctcc ttccttcggc ttgtcaaaat gaggcgtaat
      301 cttggagcgg taatggtatt ggcgggggga ggctacggcc ggattgacgg gcagttccag
      361 ccctgcctgc aagcggagca gatccgctac ctgccgggtc ttccagagaa gctggcggtc
      421 ataggaaaaa tgctggtact ggcatccccc gcaggttccg aacaggcggc agcccggttc
      481 cacgcggtcc gggctggggt taattacttc caccagatcg gcggaggagt agttcctgtc
      541 attacgccag acgcgcgcct ttacacggtc tcccggcagg gcgaagggga caaaaaccac
      601 ccatccgtcc acgcggccaa tgccgtcgcc tgcattggaa agggcgtcaa tatccagttc
      661 aagctcctgg tggtaggaaa aaggttcggg gtggaaaccc ttgggaatgg tcgcagtcat
      721 ggtggtggga aaagattatg gggcggaagg ggtaatcaac ttgccgtcca gatcgtacag
      781 cagggtttcc ggcagccggg gggtgcgcag tccggggcgg aaatcgcccg gcaagacagc
      841 cggtcccgga gcggactctt cattctcatc ctctggaaga atctcctgtt gcgccggagc
      901 gggcgcccag ggtgcgggct ggttggctga agcggtttgt tccggctgaa cggcaggaac
      961 tggggcgggg tcctgaggtg ccgtttcctt gttggaggcg caggaagagg tccaggccag
     1021 cccggcaatc agacaggagc ggatcaggag ctttctcatg gcgcggattc ttacagcttc
     1081 attcggactt ggcaagtgaa tagccctgct atggttcatg taaagggcat ttcttttttc
     1141 ccggaaaccg ggaaacgctt gctgtttgac tatcccggga tagtatctat agtaggaaca
     1201 cgtatgaggc ctttcctttt tttatgttgt tccgctgcgg tttcagggct gtttgcccct
     1261 caggccggca tagctcaaca gcgtcctccc attttcgggt ttgccaataa agacaggata
     1321 cctgaagtta ataagtcggg cagtcccgtt tttgataaac tccgtaatga agctatgaag
     1381 aaaaagcagc cgctggtaat ttatcttact ggttcttcgt ggtgttccca gtgcaatatt
     1441 tttacccggg aatatatcaa aaaaacaaat ttcaaaaatg ccgccgggaa aaaatttata
     1501 ttttggctgg tggataccaa acaggtgccg ggcaattctc cggggacgtt tcaatttaaa
     1561 atggttccgg aagaagccgc aaaaatcgtg gggtgtgtgg attctccccg cgcgccgtat
     1621 gtggttcttg gacctcccgc cgtgtttatc attgatcctg tttccggaaa attgatcaag
     1681 agcctgttct ccaagagtga agtggacaag tatggaaaac ctcttgccgg aattattgaa
     1741 gaaacctggg tggagttttc ccgcaagccc aaataggaaa cggaatgtat gcggggaacg
     1801 tccggtattt tcatgctcca gccaagatga cggggaacaa ttccaaaagt tcttttgttt
     1861 atttatagga agcgaaaaaa tcctcccggc atccctccgg ctcctttaaa aaaggcagcg
     1921 tgcggaatct ttttccgggg cggagcgatt gccgttaaaa acggtaaatg cagcgcagcc
//
LOCUS       DAOMSU010000362         1961 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6232_length_1961_cov_2.896642, whole genome shotgun sequence.
ACCESSION   DAOMSU010000362 DAOMSU010000000
VERSION     DAOMSU010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1961
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6232_length_1961_cov_2.896642"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1961
                     /locus_tag="PK077_10680"
     CDS             <1..>1961
                     /locus_tag="PK077_10680"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HRN24804.1"
                     /translation="AKNADCPFISAEGGRADIVEAAEVDSIQTQADYKNWKAALAAIE
                     QELRRAIEFGFNKEELAEARSNITAAAENAIKSWATAKSEDLASAIAQSAARDKVFTT
                     PQEDWAISREVVENLTPEQCQAALKEAWTGAFPRVIVTSNKENSQGSAEIMKAYRESQ
                     TAKVQPYQADGRKDFSYKFGDPGKVTARTETTDLGVTQLTLSNGVRVNLKPTEFDKDS
                     INITFAVDGGELSRPEKASGLELFANAVMNGGGLKDHSNDELAAIMAGKKVGVGFSMT
                     DRFFLLSGNTNREDLETQLQLQTAYLMHPGYRQDGVTLLRRAIPMIYNKMDHEVQGAM
                     KKQVPAILYRNNPRFTFPTQEQLTSYQIKDVRDWVDAPLKNNYMEVTVTGDFRTEDII
                     PLLERTVGAVPKRAEAPATLDETLRHPAMADFNFSKDLTYDSSIDKTMVCLFWKTPGG
                     ENKKLARRLNMLKAVFYDRVFKGLREDMGETYSPSTGLNISETYPDDGYIITMSSGVM
                     RNKEAVRSAIARIADDLGKGNITQEELDRARNPILNSMDRAQRDNGYWTSLLKDSQAR
                     PERLNQQRESIPDVKAITVEEVNKLAKDIFGKGEHLNLNILPDHPAAETPPAEKQADK
                     ADSPQAAVSPAAFCVRTTVIKTVNKDSGK"
BASE COUNT          506 a          595 c          533 g          327 t
ORIGIN      
        1 ggcgaagaat gcggactgcc cctttatctc cgcggaagga ggccgcgcgg acattgtgga
       61 agcggcggaa gtggattcca ttcagaccca ggcggactac aagaattgga aagcggctct
      121 ggccgccatt gaacaggaac tgcgccgggc cattgaattc ggcttcaaca aggaagaact
      181 ggcggaggcc cgcagcaaca ttaccgccgc cgcggaaaac gccatcaagt cctgggctac
      241 cgccaagtcg gaagacctgg cttccgccat cgcccagagc gccgcacggg acaaggtctt
      301 caccacgccg caggaagact gggccatctc cagggaagtt gtggaaaacc tgacacccga
      361 acaatgccag gcagccctga aggaagcctg gaccggagcc tttccccgcg tgatcgtcac
      421 ttccaataag gaaaactccc agggaagcgc cgaaatcatg aaagcgtacc gggaatccca
      481 gaccgctaaa gtacagccct accaggcgga cggccgcaag gatttttcct ataagttcgg
      541 ggatcccggc aaggtgacgg cccgtacgga aaccaccgac ctgggagtca cccagctcac
      601 cctgtccaac ggcgtgcgcg tcaacctgaa gcccacggaa tttgacaagg actccatcaa
      661 catcaccttt gccgtggacg gcggggaact gagcaggccg gaaaaagcct ccgggctgga
      721 attgtttgcg aatgccgtca tgaacggcgg cggtttgaaa gaccactcca atgacgaact
      781 ggccgccatc atggcaggca agaaggtagg ggtaggcttc tccatgacgg accgcttctt
      841 cctgctttcc ggaaacacca acagggaaga cctggaaacg caactccagc tccagacggc
      901 ctacctgatg cacccaggtt accgccagga cggcgttacc ctgctccggc gggccattcc
      961 catgatttat aacaagatgg accatgaagt acagggagcc atgaagaagc aggttccggc
     1021 catcctttac aggaacaatc cccggttcac cttccccact caggaacagc ttacctccta
     1081 ccagatcaag gacgtgcggg actgggtgga tgccccgttg aaaaacaact acatggaagt
     1141 gaccgttacg ggagacttca ggacggaaga catcatcccc ctgctggaac gcaccgtagg
     1201 cgcagttccc aaacgtgcgg aagctccggc aacgctggat gaaacgcttc gccatccggc
     1261 catggcggac ttcaatttct ccaaggacct gacctatgac tcctccattg ataaaacgat
     1321 ggtctgcctt ttctggaaaa cgcccggcgg agaaaataaa aagctggccc gcaggctgaa
     1381 catgctcaag gcagttttct atgaccgcgt attcaagggc ttgcgcgaag acatggggga
     1441 aacctactcc ccttccaccg gcctcaatat cagtgaaacc tatccggatg acggctatat
     1501 tatcacgatg agttccggcg tcatgcgcaa taaggaggct gtgcgcagcg ccatcgccag
     1561 aattgcggac gacctcggca agggaaatat cacccaggag gaactggacc gtgcccgtaa
     1621 tcccattctt aactccatgg accgcgccca gcgcgacaac ggctactgga catccctgct
     1681 gaaagactcc caggccaggc cggaacggct gaaccagcag agggaaagca ttccggacgt
     1741 aaaagccatc accgtggaag aggtcaacaa acttgccaaa gacatcttcg gcaagggaga
     1801 gcatctcaac ctgaacattc tgccggatca cccggctgcg gaaactccgc ctgctgaaaa
     1861 gcaggcggac aaagccgact ctccccaagc cgccgtctct ccggcggctt tttgcgttcg
     1921 caccaccgtc ataaagactg tcaacaaaga ctccggcaag a
//
LOCUS       DAOMSU010000363         1958 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6250_length_1958_cov_2.110352, whole genome shotgun sequence.
ACCESSION   DAOMSU010000363 DAOMSU010000000
VERSION     DAOMSU010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6250_length_1958_cov_2.110352"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..1754
                     /locus_tag="PK077_10685"
     CDS             126..1754
                     /locus_tag="PK077_10685"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HRN24805.1"
                     /translation="MPNAPHFQDSILLGASYGFIIRNLARENRFSTGQLPEAQQPEQY
                     YKESNQMKNGIMMQYFEWNLPNDGQFWNKLKDDAPHLEEMGVTAVWIPPAYKGKEQND
                     VGYGTYDLFDLGEFDQKNTVRTKYGTRQELQEAIKALHEHHVGVYLDAVMNHKAGADY
                     TEKFMAKEVDQQNRDKEITDAYEIEGWTGFNFPGRGNKYSDFKWHWYHFTGTDYDART
                     EKTSIFKIMGDGKSWSEGVDEENGNYDYLMFANLDFNHPEVVKEMERWGIWVSRELDL
                     DGMRLDAIKHINDEFIRKFLAAVRKERGADFYAVGEYWKQDLESLDDYLKEERYKVDL
                     FDVPLHYNMYQASKQGRDYDLSKILDGTLVQNHPTLAVTFVDNHDSQWGSSLESAVED
                     WFKPSAYALILLMKEGYPCIFYGDYYGVSGNPPMHRAIIDNLLEIRKNHAFGEQNYYF
                     DHPNTIGFTRVGDEEHPHSGVAVLISNGEDGDKVMNVGKQHAGETWKEATGNVEETVS
                     IDGEGNGRFLVHGGNVAVWIPENALQDARKEDGK"
BASE COUNT          533 a          523 c          518 g          384 t
ORIGIN      
        1 agggcacatt ctttccgggg tgcggcaggg ggggagtgac accgcttttt cccatcctcc
       61 agagaaaagc cctccccgcc taccgttttg ccgctggcat ttttgcagga cataagaccg
      121 gcagtatgcc taacgcgccg cactttcagg actctattct gttgggggct tcgtatggat
      181 tcatcatccg gaacctcgcc cgggaaaacc gcttttccac cggacagctt ccggaagcgc
      241 aacaacccga acagtattac aaggaatcaa atcaaatgaa aaacggaatc atgatgcagt
      301 attttgagtg gaacctgccc aacgacggac agttttggaa caagctgaag gatgacgccc
      361 cccacttgga ggaaatgggt gtcacggccg tctggattcc tccagcctat aaagggaagg
      421 aacaaaatga tgtaggatac ggcacctatg atctttttga cctgggggaa ttcgaccaga
      481 aaaacaccgt ccgcaccaaa tacggcacca ggcaggaact ccaggaggcc atcaaggctc
      541 ttcatgaaca ccatgtaggc gtttatctgg atgccgtcat gaaccacaaa gcgggagcgg
      601 actatacgga aaaattcatg gccaaggaag tggaccagca gaaccgcgac aaggaaatta
      661 cggacgccta tgagattgaa ggctggaccg gcttcaactt tcccggccgc ggaaacaaat
      721 attccgactt caaatggcac tggtaccact tcaccggaac ggactacgac gcgcgcacgg
      781 aaaaaacctc catcttcaag attatgggag acggcaaaag ctggagcgaa ggcgtggatg
      841 aagaaaacgg caactacgac taccttatgt tcgccaatct ggacttcaat cacccggaag
      901 tggtgaaaga aatggaaaga tggggaatat gggtatcccg ggaactggat ctggacggca
      961 tgaggctgga cgccatcaag catatcaacg acgaattcat caggaaattc ctggcggccg
     1021 tccgcaagga aaggggagca gacttctacg ccgtgggcga atactggaaa caggatctgg
     1081 aatccctgga cgattacctc aaggaggaac gctacaaagt ggacctgttt gacgtccccc
     1141 tgcactacaa catgtaccag gcctccaagc agggccgcga ttacgacctt tccaaaattc
     1201 tggacggaac cctggttcag aaccacccta cgctggccgt tacctttgtg gacaaccatg
     1261 attcccagtg gggcagttcc ctggaatcag ctgtggaaga ctggttcaaa ccctccgcct
     1321 atgcgctcat cctgctcatg aaggaaggtt atccctgcat cttctacggg gactattacg
     1381 gtgtgagcgg caatccgccc atgcaccgcg ccatcatcga caatctgctg gaaatccgta
     1441 aaaaccatgc tttcggggaa caaaattact actttgacca cccgaacacc atcggcttca
     1501 cccgtgtggg agatgaagag cacccgcatt ccggcgtggc tgtgctcatt tccaatggag
     1561 aggatggaga caaagtcatg aacgtgggca agcagcacgc aggggaaact tggaaggaag
     1621 ccaccggcaa cgtggaggaa accgtctcca ttgacgggga aggcaacggc cggtttcttg
     1681 tccacggcgg caatgtggcc gtatggattc cggaaaacgc tttacaggac gcccggaagg
     1741 aagacggaaa ataacgcaac tcccgcccgc aaattgaaga aacggcctgt tctccattcc
     1801 ggactgtttt tccggaatga ggttcaggcc gttctgactt taatcaccag tttcagaatc
     1861 tttccttccc caatgcagcc ggcatgcgca tcctccggga aaaaaatggc gaactgcccc
     1921 ggtttcacga caaaagaggt gtccggagca tcatcata
//
LOCUS       DAOMSU010000364         1957 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6253_length_1957_cov_2.676656, whole genome shotgun sequence.
ACCESSION   DAOMSU010000364 DAOMSU010000000
VERSION     DAOMSU010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1957)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1957
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6253_length_1957_cov_2.676656"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            624..>1957
                     /locus_tag="PK077_10690"
     CDS             624..>1957
                     /locus_tag="PK077_10690"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRN24806.1"
                     /translation="MKGFIKLIIVIAVLGGIWFAWEQWKGNEAIQVDYQTEPLEKGDL
                     MITIEATGVTEPDELVDVGAQVSGIIMEFGKDMNGKVVDYSSPVKAGQMLAEIDKLPV
                     QLDVQRAEASKAQAIAGIARSKADIQQAKAKHQQARLDRERAEKLGPGDALSKSSYDQ
                     YIADEETARANVAVAEASLQEAEASLKQAEAALKKEMRNLEYTTIKSPVDGVVVKRLV
                     NIGQTVVSSMSASSLFYIATDLSKLKIWAAVNEADIGSIRKGQEVLFTVDAFAGRKFK
                     GTVDKIRLDATMTSNVVTYIVDIDVPNPDKLLIPYLTANVQFVVEDIRNAFLVSNAAL
                     RFRPETELVSAEQKAAFDRMQPELAAPVRDGQKKKAVVWELRDNLLYPHLVTKGESNG
                     MLTAIAGETLREGMEIVSTAQILNRSDSSGDGSSASAGGENPFAPKMPPRRKP"
BASE COUNT          507 a          550 c          517 g          383 t
ORIGIN      
        1 ccttggaaca ttccctacca tgaggtatat aaggtggtgg ctgctgcact cgtcatcgcc
       61 tggcaaagtg gctcggaccg atattacaac ctaatggggg cagccccgac gcagggctga
      121 ctacgtccta taccggaaaa gtcaaaacag cggcggaagt ccccgctcta ttgaggcacg
      181 ggaacgtggc tcacagccag agacggtgca gcggtgtctt ttttccatca cctgaatcca
      241 gaaagctcgt tctttcaacg tttttctcct ccttgaatcc gcaggtaagc cgggattccc
      301 ctcttgggca acattactcc tgtcgggatt caccgccttt ccctgttctt cccacccccg
      361 catcacttac aaacacggag aagccaattc cccaaaagat cgaatgggag catcttcaga
      421 tatttcgtgg ccctccattc ccgctccttc cgtcactggg cgagaaggaa gaaaggaaat
      481 attattagtt aataaacagc aactgttaat aaacataata atcccttaga catttccgga
      541 aatcatgata ggatcacgct ccgttgcgga aaagttttta acatccgtgc aactttttca
      601 ttattgcgaa gtataatata gtcgtgaagg gattcatcaa actcatcatc gtcattgccg
      661 ttctcggagg catctggttt gcctgggaac agtggaaagg aaatgaagcc atccaggtgg
      721 actaccagac agaacctctt gaaaaaggag acctgatgat cactatcgaa gcaaccggcg
      781 ttacggagcc tgatgaactg gtagatgtag gcgcccaggt cagtggaatc atcatggagt
      841 tcggcaagga catgaacggc aaggtagtgg attattccag ccctgtaaaa gccggtcaga
      901 tgctcgcgga aatcgacaag cttcccgtgc aactggacgt tcagagggcg gaagcctcca
      961 aagcgcaggc cattgccgga atcgcccgct ccaaggcgga catccagcag gccaaagcca
     1021 agcaccagca ggccagactg gaccgggaac gcgcggaaaa gctggggccg ggggatgccc
     1081 tgtccaaatc cagctacgac cagtatattg cggatgaaga aaccgcccgc gccaacgtag
     1141 ccgtggcgga agcctccctg caggaagcgg aagcatccct gaaacaggcg gaagccgcgc
     1201 tgaaaaagga aatgcgcaat ctggaataca ctaccatcaa gtcccccgtg gacggcgtcg
     1261 tggtcaaacg cctggtcaac ataggccaaa cagtagtttc cagcatgagc gcctccagcc
     1321 tgttttacat cgccacagac ctttccaagc tgaaaatttg ggccgctgtg aacgaggcgg
     1381 acatcggcag catccgcaag gggcaggagg tgctgttcac cgtggacgct ttcgccggcc
     1441 gcaaattcaa gggaacggtg gacaagatac ggctggacgc aaccatgacg tccaatgtgg
     1501 tcacttacat cgtggacatt gatgtaccca atccggacaa gcttcttatc ccctatctga
     1561 cggcaaatgt ccaattcgtg gtagaggaca tacgaaacgc cttcctcgtt tccaatgcgg
     1621 ccctccgctt ccgcccggaa acagaactgg tcagcgctga acaaaaagcg gcctttgacc
     1681 ggatgcagcc ggagctggcc gcccccgtcc gggacggaca aaagaaaaaa gccgtggtct
     1741 gggaattgcg ggacaatctt ctttatcccc acctggtcac caaaggggaa agcaacggca
     1801 tgctgacagc cattgcggga gaaacgctcc gggaaggaat ggaaatcgta tctacggccc
     1861 agatactgaa ccgctccgac agttcggggg acggttcttc cgcctccgcc ggcggggaaa
     1921 acccgttcgc gcccaaaatg cccccgcgcc gcaagcc
//
LOCUS       DAOMSU010000365         1937 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6352_length_1937_cov_2.983528, whole genome shotgun sequence.
ACCESSION   DAOMSU010000365 DAOMSU010000000
VERSION     DAOMSU010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1937
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6352_length_1937_cov_2.983528"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="PK077_10695"
     CDS             <1..415
                     /locus_tag="PK077_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRN24807.1"
                     /translation="RNASGTDSVPSYEEFCAAFPELGIFQPAWDSLNDDLNTPEALGH
                     VFSAIRKADIPSLSPEEAARLRNAFHFILAAFGIILPEEGQEEAPEEIRTLADQRWQA
                     KQNRDWTEADRLRAEVAALGWVIKDRKDGYDLARK"
     gene            420..755
                     /locus_tag="PK077_10700"
     CDS             420..755
                     /locus_tag="PK077_10700"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRN24808.1"
                     /translation="MDAFSSIPNHTGFTARNLFMDRQGILRGGAFAKIAPGGGPLSPH
                     RHAHAHLFIVTRGTISVMLDGEERTVHEYESQLVPGGVLHAVWNRNTEPAEILGLTLE
                     TPSSPTSSI"
     gene            755..1669
                     /locus_tag="PK077_10705"
     CDS             755..1669
                     /locus_tag="PK077_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRN24809.1"
                     /translation="MSSRQRTLGHAAALMTILIWGTTFVSTKVLLRDFTPVTVLFTRF
                     VIGYAFLWCLKPRFLPFSGWKKEFLFAGAGLTGVTLYFLLENIALTYTFASNVGIIVA
                     VVPFFTALLAHFLLKGEGFSRRFFLGFAAAFTGIFLIMANGAFVLELNPAGDILALGA
                     AFVWAAYSILMKKIGVNTSNMIICTRRIFFYGIALMIPALWLLPANMDWHLMVKPVNA
                     FNLLYLGLFASALCFLTWNRVVEILGAVKSSIYIYMVPVVAVLASAIILGERLTWISL
                     TGILLTLCGVTISEYRKKTRKSGKKRPS"
BASE COUNT          414 a          564 c          490 g          469 t
ORIGIN      
        1 caggaacgcc tccggaacgg attccgttcc ctcctatgag gaattctgcg cggcattccc
       61 ggaattggga attttccagc cggcatggga cagcctgaac gatgacctaa acactccgga
      121 agccctgggc catgttttca gcgccatcag gaaggcggat atcccctccc tttcaccgga
      181 ggaggcggcc cgcctgcgga atgccttcca ctttattctg gccgccttcg gcattattct
      241 gccggaggag ggacaggagg aagccccgga agaaatccgc accctggcgg atcagcgctg
      301 gcaggccaag cagaaccggg actggacgga agccgaccgc ctgagggcgg aagtggcagc
      361 gctgggctgg gtcattaaag accgcaagga cggatacgac ctggcacgca aataaacaaa
      421 tggacgcctt ttcctctatt cccaatcata cgggcttcac ggccaggaac ctgttcatgg
      481 acaggcaggg aatcctgaga ggcggagcct tcgccaaaat agcccccggc ggcggccccc
      541 tttccccgca ccgccatgcc cacgctcatt tattcattgt cacccgtgga acgatttctg
      601 tcatgcttga cggagaagaa agaacggttc acgagtacga atcccagctc gttcccggcg
      661 gcgttctcca tgccgtctgg aacaggaata cggaaccggc ggaaatcctg ggactgacgc
      721 tggaaacgcc ttcctccccc acatcgtcaa tttaatgagt tcccgacagc gcaccctggg
      781 ccatgccgcc gccctcatga ccattctcat ctggggaacg actttcgtct ccaccaaggt
      841 gcttttgcgg gatttcacac ccgtcacggt actcttcacc cgctttgtaa taggatatgc
      901 ttttctctgg tgcctgaaac cgcggtttct tccattctcc ggttggaaaa aggaattcct
      961 gttcgccgga gcgggattga ccggggttac cctgtacttc ctgctggaaa acatcgccct
     1021 gacctatacc tttgcctcca atgtggggat tatcgtggca gtggttcctt tcttcacagc
     1081 ccttctggct catttcctgc tgaaggggga aggcttttcc cgccgtttct tcctgggttt
     1141 tgccgccgcc tttacgggta tcttcctcat catggcaaac ggagccttcg ttctggaact
     1201 gaatccggcg ggggatatcc tggcactggg cgcggccttt gtctgggctg cgtattccat
     1261 tctgatgaaa aaaatcggag tcaacacgtc caacatgatc atctgtacgc gccgcatttt
     1321 tttctatggc atcgcgctga tgattcccgc cctgtggctc cttccggcaa acatggactg
     1381 gcatctgatg gtaaaacccg tcaatgcctt taatctgctg tatctgggcc tgttcgcttc
     1441 cgcactttgt ttcctgacct ggaaccgcgt ggtggaaatt ctgggagccg tcaaatccag
     1501 tatttatatt tacatggtac cggtcgtagc cgtgctggcg tcagccatca ttctggggga
     1561 acgcctgacc tggatatccc tgacagggat cctgctcacc ctgtgcggcg tcacgatttc
     1621 agagtataga aaaaaaacac ggaaaagcgg aaagaaaagg ccatcctgag aaaaatccga
     1681 tttctcccgc tccggttctc tatatttttc ccccttgcat ggagatggac agaaaccgct
     1741 tccagaacat gtcaggccga tgatttcccg caacggacag acattcattt atcacgttgc
     1801 ggaaaaacct gtctctggat tagatatccg gcaacaacaa agttttttga aaaaaatcct
     1861 ctcagcttcc ggaaacgttt ctttccgcct cttcttgaat tcgggcttcc gtctcttcaa
     1921 tcttttccga aaggtca
//
LOCUS       DAOMSU010000366         1928 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6399_length_1928_cov_2.849439, whole genome shotgun sequence.
ACCESSION   DAOMSU010000366 DAOMSU010000000
VERSION     DAOMSU010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6399_length_1928_cov_2.849439"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="PK077_10710"
     CDS             complement(<1..943)
                     /locus_tag="PK077_10710"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HRN24810.1"
                     /translation="MNQAPHVAIVGATGAVGVEILSCLETRNFPVGSLKLLASARSAG
                     KQVAFRGKMLTVEELTEKSFDGVDIALFSAGGGISLKFAPVAAAAGCVVIDNSSAFRQ
                     EPDVPLVVPEINPEAAFNHPRNIIANPNCTTIITLMALFPLHQRFGLKTVIASSYQAV
                     SGSGQHGIAELEAQVRAVVDGHPVVKNVYPHQIAFNLLPQIDSFAENGYTKEELKMLN
                     EGRKILSLPELKVTCTCVRVPVYRSHSIAVTAQFEKPVDVETARSAYEGKPGVALMDN
                     PAEGVWPTPLDSTNGDTCYVGRMRMDMAIDNALTLWVV"
     gene            complement(1109..>1928)
                     /locus_tag="PK077_10715"
     CDS             complement(1109..>1928)
                     /locus_tag="PK077_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HRN24811.1"
                     /translation="NLRLTSGLKIRFIHHERVQDSPANYSNKKEQRTTMKNTLFLSGL
                     MALCCGAGMAATVAIDLNGEAAEGASLPANLKLSGFGNVNSGSVITAQKVVTWLQGTT
                     GWQGTTANGNSGSGIYGSVTASGDTDVSVSFINRNAYGGSIVGTAVTLTSDYASYTGL
                     TLSVDCAGLQSGANFTTLNIVYQTAEGWQISQLTGQASSLIQTGMTLSVDLSGTPLTS
                     DTAYIVMSTRNGGGNTNSNLLFSMSATAEDVVPEPATASLSLLGLSLLMLRRKR"
BASE COUNT          423 a          497 c          570 g          438 t
ORIGIN      
        1 ccacgaccca gagagtcagg gcattgtcaa tcgccatgtc catgcgcatg cgccccacgt
       61 agcaggtgtc tccgttggtg ctgtccagcg gggtaggcca cacgccctcc gcagggttgt
      121 ccatcagggc gactccgggt ttgccttcat aggcgctgcg ggccgtttcc acgtccacgg
      181 gtttttcaaa ctgggcggtg acggcgatgg aatgggaacg gtaaacgggc acgcgcacgc
      241 aggtgcatgt taccttgagt tcgggcaggg aaagaatttt acggccttca ttaagcatct
      301 taagctcttc cttggtatag ccgttttccg caaaggagtc gatctgggga agaaggttga
      361 aggctatctg gtgaggataa acatttttca ctacagggtg gccgtccacg acggcgcgaa
      421 cctgggcttc cagttccgcg atgccgtgct ggccgctgcc ggaaaccgcc tggtaactgg
      481 aggcaatgac ggttttcaac ccgaaacgct gatgcagagg gaagagggcc atcaacgtga
      541 tgatggtggt gcagttcggg ttggcgatga tattgcgggg gtggttgaaa gccgcttccg
      601 ggttgatttc cggcaccacc aggggcacgt ccggctcctg ccggaaggcg gaggaattat
      661 caatgaccac gcagcccgca gcagccgcga caggagcgaa tttcagggaa atgcctccgc
      721 ctgcgctgaa cagggcgatg tccaccccat caaaggattt ttccgtcaat tcttccacag
      781 tcagcatttt cccgcggaaa gcaacctgtt ttccggcgga ccgggcggag gccagaagct
      841 tcagggagcc tacgggaaaa ttgcgtgttt ccaggcagga caggatttca acgcccaccg
      901 ctccggtggc gccgacgatg gcaacatggg gtgcttggtt catgattgtt gtcagaaaat
      961 gacggatatt ttccgtccgc ccccattata cggcagcccg gtcatatggc aatacgattg
     1021 caagccttca ggcttaaaaa tgaaaaactg tccggggtgt agaatcccgg acagttgttg
     1081 aaaaacggga aagatcgcgt tttttatttc agcgcttgcg gcgcagcatc agcagggaca
     1141 ggcccaaaag gctcagggat gccgtagccg gttccggaac gacatcctca gcagtggctg
     1201 acatggaaaa aagcaggttg gagttcgtgt ttccgccgcc attgcgggtg gacatcacga
     1261 tgtaagccgt gtcgctggta aggggagtac cgctcaagtc cacggacagg gtcatgcctg
     1321 tttgaatcag ggaagaggcc tgaccggtca actggctgat ctgccagcct tctgccgtct
     1381 gataaacgat attgagcgtc gtgaagtttg ctccggattg cagcccggcg cagtccacgg
     1441 aaagagtcaa tccggtatag ctggcgtaat cactggtaag cgttaccgct gttcctacga
     1501 tagagccgcc gtaagcattt ctgttgatga aagacacgga tacatcagta tccccggatg
     1561 cggtgacgct gccgtaaata ccggagccgg aattgccgtt tgcagtagtg ccttgccagc
     1621 cggtggtgcc ttgcaaccat gtgacgactt tttgtgcagt gatgacactt ccgctgttga
     1681 cgtttccgaa tccagacaat ttaaggtttg caggcagaga tgccccttcg gctgcctccc
     1741 catttaggtc gattgctacg gtggcggcca tccctgcgcc gcaacacaaa gccataagac
     1801 cggaaagaaa taatgtgttc ttcatggttg ttcgttgctc ttttttattg ctgtaatttg
     1861 ccgggctgtc ttgaacccgc tcatgatgaa taaagcggat tttcaatccg gaagtcaagc
     1921 gtaaattc
//
LOCUS       DAOMSU010000367         1916 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6461_length_1916_cov_2.828587, whole genome shotgun sequence.
ACCESSION   DAOMSU010000367 DAOMSU010000000
VERSION     DAOMSU010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6461_length_1916_cov_2.828587"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1778)
                     /gene="dxs"
                     /locus_tag="PK077_10720"
                     /pseudo
     CDS             complement(1..1778)
                     /gene="dxs"
                     /locus_tag="PK077_10720"
                     /inference="COORDINATES: protein motif:HMM:NF024688.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
BASE COUNT          362 a          479 c          619 g          456 t
ORIGIN      
        1 tagcgcggct tccgccgtga gtccatattt ttttctgagg atggattccg aaccatgctc
       61 aatgaattgg tccggccaag cgatggcttc cacgggcgtt ttgacatctc cggtttccag
      121 ggattcgaga acggctgagt tgaagccgcc gctgatggag tggtcttcga aggtgcagac
      181 cacccggctg cgtgctgcgt gacggcggat gcattcatca tccagaggtt tgatgaaacg
      241 ggcattgatc agggtgacgg tgtagccgcg ggcttccagc agcgaggcgg tttcccgggc
      301 gatgccgatc atggtgccca gggaaacgag ggttacatcc cgtcccgtct gaagtacttc
      361 cgccttgccg atggggagga tttccgcctt ggcggggaca ggggttccgg ctccgcatcc
      421 cctggggtag cggataacgg tggggccgtt ctggtaatgg ttcatggttc ggagcatgtg
      481 gacgaattcg gcttcatcct tgggctgcat gaatataatg ttcgggatac tgcgaatcat
      541 ggcaatgtca aacagcccgt ggtgtgtggg gccgtcgtcc ggggaaagcc cggcgcggtc
      601 catgcagaaa cgcacgggca gtttttgcag cgcggcgtca tgttgaatca tgtcaataca
      661 gcgctgcatg aaagtggagt aaatggcgat gtagggtttc atgccctccg ccgccagccc
      721 gcaggcgaag agggcgccgt gctcttccgc gatccctacg tcgaaatagc gtttggggaa
      781 ggcttccttg aataagtcca gcttggtgcc gctggccatg gcggccgtga tggctgtgat
      841 ggattcatcc tcgcgcgcca tttccaccag cgtggagccg aagatatggg agaatgtggg
      901 ggtgggggcg gcctgcgttt ccccgtccct cacgcaatag gagcccaggc cgtggaattt
      961 ggtggggttg tccagagcgg gctggtagcc cttacccttc tgagtgacca catgcagaat
     1021 aacgggttcg ttgagatcct tgatgtaggc aagggtgcgt atcagggtgg gaatgtcgtg
     1081 tccgtccagg gggccaaaat agcgcagccc gaatttgttg aacagcaggg acgggaagat
     1141 aaggtttttg gtggtgcctt ccagcttgaa agcaaagtcc ctggcctgcg tgccgcccag
     1201 cttttcaatg aaggaagccg ttttatccct taaccaggaa aacgtttccg atgtttgcag
     1261 ggagttgaag tattttgcca gcgctcctac gtttttgtcg atggcccatt cgttgtcatt
     1321 cagaatggtg atgtaccgtt tggtggtctg gctgatattg ttcagcgcct ccagagtggt
     1381 gccgcaggtg aatgccgcat ccccggctac ggccaccaca tggtagtctt ctcctttcag
     1441 gtccctggcc gcgcacatgc cgccgtgccc gcatgccccg ctccgtagga gtcatgaggg
     1501 gattcggaca ttttggcaaa tccggaaagc cccccgtgct ggcggatggt gtggatcagg
     1561 ggagcgcgcc cagtcagcat tttatggaca taggcctggt gggagacatc aaagatgatt
     1621 ttatcccgcg gcgtttcaaa gacgcggtga agggcaatgc tcagttctac cacgcccagg
     1681 ttggggccca ggtggcctcc ggtgagggaa agggagtgaa tgagagtatt ccggatttct
     1741 tccgcaagcc tgggcagttc cgactcaggg atttccatca gatctgcgtg actgcggatg
     1801 cttgccagca gttccggaag ttcaggtgag ggagaattca ttgctgtctg gttcatcagt
     1861 atccgtttta tcctgaacgg tttccgggtt gctgagagtt tgaatgcgca gttccg
//
LOCUS       DAOMSU010000368         1888 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6619_length_1888_cov_2.099291, whole genome shotgun sequence.
ACCESSION   DAOMSU010000368 DAOMSU010000000
VERSION     DAOMSU010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6619_length_1888_cov_2.099291"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="PK077_10725"
     CDS             complement(<1..629)
                     /locus_tag="PK077_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRN24812.1"
                     /translation="MSRASRTERLDYLKDQLHKRVVILDGAMGTNIQKFRLGEEDYRG
                     ERFADAARYPNDLKNNNDLLVLTRPDIILDIHRRFLDTGRTDILETCTFGATSIGQHD
                     YFWHRPEKGSHKNQQYFQEVVDSPELKALVRELNLAAAALARQACDEAEAADGKPRLV
                     AGSIGPMPVTCSLSPDVNDPGFRAVNFRQLRQAYRDQALALLEGGVDMLL"
     gene            1095..1556
                     /locus_tag="PK077_10730"
     CDS             1095..1556
                     /locus_tag="PK077_10730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24813.1"
                     /translation="MKSGLQLGNGPVFSLGFGAYNTQTTAFGFSTTTNTWAFSNNGNN
                     MNVSGKSGSVSDIYSGMLTVNFLAENGSTYVEAFLGDTALVNKTEINYEKSVLYLNGL
                     TLGGWAQHIRQRLFHDPSVPFPSARFRNRRYPLWRSWDWEAFWQGAADINV"
BASE COUNT          442 a          464 c          465 g          517 t
ORIGIN      
        1 agcagcatgt ccaccccccc ctccagcagg gccagcgcct gatcccggta cgcctgcctg
       61 agctggcgga aattcacggc gcggaagccg ggatcattca catcggggga cagggaacag
      121 gtgacgggca tggggccgat ggacccggcc accaggcggg gcttgccgtc tgcagcttcc
      181 gcttcgtcgc atgcctgccg ggcgagcgcg gcagccgcca ggttcaattc ccggacaagg
      241 gctttcagtt caggggagtc taccacctcc tggaaatact gctggttctt gtggctgcct
      301 ttctccggac ggtgccagaa ataatcatgc tggccgatgc tggtggctcc gaatgtgcag
      361 gtctccagaa tatccgttcg tcccgtgtcc agaaagcggc ggtgaatatc cagaataata
      421 tccggcctgg tcagaaccag aagatcgtta ttattcttta aatcgttggg atagcgtgcc
      481 gcatccgcaa aacgttctcc gcggtaatct tcctctccca gcctgaattt ttggatattg
      541 gtgcccatgg cgccatccag gatgacaacc cttttatgta actggtcttt caggtaatca
      601 aggcgttctg ttctggatgc tctgctcata tggcgcggaa aatctaccgc ccgcccgtca
      661 ttggaacaag gacaattgtc gtcacgtttt cctcgttccg ttcctgggga atggtaggaa
      721 gctgttttca ggggggtgga aaagacatgg ggatgattta tggaaagtaa aaaacgcctg
      781 tgaaaaatcg tatgtttcgt tctggatttt tcgatggcat aaggagcgtt ctctgttttc
      841 ttccctttcc atgcagtctt tcctgtttca tgtttttcct gagaaaaaag aatgcgggtt
      901 ctgaaaaaag agggaagagg tgtgaataaa atgataataa caggtgtata actggaacgg
      961 ccgttgctat tccaataacg ggaaagtcat tcatgccgga tagcggagca ggggatgaaa
     1021 gtgtactttt ccagtaaaat gatttgttgg caggcgttct tagctacact tgatgaataa
     1081 aaatcccctc agaaatgaag tccggcctcc agcttggaaa tggccccgtg ttctctttgg
     1141 gctttggagc atacaacacg caaacgacgg ccttcggttt ttctaccacc accaatacct
     1201 gggctttttc caataatgga aataacatga atgtttccgg gaaaagcggc tccgtttccg
     1261 acatctattc cggcatgctc accgtcaatt tcctggcgga aaacggctct acctatgtgg
     1321 aggcattcct tggagacacg gcattagtca acaaaacgga aatcaactat gaaaagtcag
     1381 ttctctactt gaacggtctc accttgggag gctgggccca gcacatccgg caacggctgt
     1441 tccatgaccc atcggttccc tttccatcag ccagattccg gaaccggcga tatcctctct
     1501 ggcgttcctg ggactgggag gccttctggc aaggcgccgc agacattaat gtctgattgt
     1561 ttttaccttc cccggcaaca tctggaaagg tggcaaacgc atctgctttt gtccagcaac
     1621 ccaggtctat taaaagcgct gcttcttccg tctgttgaaa ctttcccgcg ttcccgtatt
     1681 tttttccgta tattcaacat catttatacg gtcagagggt tatgagatac tgaatatatc
     1741 ccccctttct ttcagaatat tttattgatt ttctatgttt tttatagaat gaatttgctg
     1801 aatatcaaca gatggaatct tttgagaatc tcgcggcagg actattctac atcatttctt
     1861 attttttaat cgtactgaaa aatgattt
//
LOCUS       DAOMSU010000369         1887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6624_length_1887_cov_2.439410, whole genome shotgun sequence.
ACCESSION   DAOMSU010000369 DAOMSU010000000
VERSION     DAOMSU010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6624_length_1887_cov_2.439410"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="PK077_10735"
     CDS             <1..769
                     /locus_tag="PK077_10735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24814.1"
                     /translation="FMIIGSRYDLSPQEAEVLQRYWARPNAAILIMLEPQNDTPKQLY
                     RFLREQGLRPQNDRVMLRNRGNRSVFEINSIFAPSLNCTREFWNSSTGLEGESISLIL
                     DSDNAAMEQKRITPYPLLVTTEDYYGETKYNQFPAKFDAREDNPGPLMIGAALIRGNA
                     GDVNQNKTTGRLVLLGNTDLLQPRQIKPEQRDFMRTLIGWMTDREELRGLGSRHDLTV
                     KLNLDRNALGVLELLTNIGLPLLALLIALIIWNTRRH"
     gene            1056..1556
                     /locus_tag="PK077_10740"
                     /pseudo
     CDS             1056..1556
                     /locus_tag="PK077_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1686..1887
                     /gene="groEL"
                     /locus_tag="PK077_10745"
                     /pseudo
     CDS             1686..1887
                     /gene="groEL"
                     /locus_tag="PK077_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005920153.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; many bacteria have multiple
                     copies of the groEL gene which are active under different
                     environmental conditions; the B.japonicum protein in this
                     cluster is expressed constitutively; in Rhodobacter,
                     Corynebacterium and Rhizobium this protein is essential
                     for growth; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
BASE COUNT          419 a          610 c          485 g          373 t
ORIGIN      
        1 attcatgatt atcggttcca ggtatgatct gtccccgcag gaggcggaag tgctccagcg
       61 gtactgggcg cgaccgaacg ccgccattct gatcatgctg gaaccccaga atgacacccc
      121 caaacagctt taccgctttc tccgcgaaca ggggctgcgg ccccagaatg accgcgtgat
      181 gctccgcaac aggggcaacc gttccgtttt tgaaattaac tccattttcg ccccctccct
      241 gaattgcacc cgtgaattct ggaattccag caccggactg gaaggggaga gcatctccct
      301 cattctggat tccgacaacg cggccatgga acagaagcgc attacgccgt atccccttct
      361 ggtgactacg gaggattatt acggagaaac caaatacaac cagttcccag ccaagtttga
      421 cgcaagggaa gacaatccgg ggcctctgat gatcggcgcg gcccttatcc gggggaatgc
      481 cggagacgtg aaccagaaca agactaccgg acgcctggtt ctgcttggca atacggacct
      541 gcttcagccc aggcaaatca aaccggaaca aagggatttc atgcgcacgc tgatcggctg
      601 gatgacagac cgtgaagaat tgcgcggcct cggctcccgc catgacctga ccgtcaagct
      661 gaatctggac cgcaacgccc tgggcgtttt ggaactcctg acgaatatcg gccttcccct
      721 gctggcgctg ctgatcgccc tgattatctg gaacactcgc cgtcattaac cccatccacg
      781 cccattaccg gaagcacatg cgcacattcc gctttatcct tctcatcctc atcgccctcg
      841 cttctgtcgg cgcggcggtg ctcctcacca ttgacggcaa cctgtcccgc atcatcgggc
      901 gcaccgcctt cagttccgga gaacggcttt tcccatacac ccgggaagaa atgaacgagg
      961 tttcctggat gcgcatcaat tgcgtggggg atgtcgcgga attccgccgt aagcccaacg
     1021 gcgtctggtg gtgcgaaaaa ccctggaacg accgtatgga ccctcgcgcg gcggcggcca
     1081 ttctccagta cacgtattcc accagcattg tggacgcgct gcctctgcac aaaattgatt
     1141 ccgcttccct gaaggaattc ggcgtaaaga ccacccccgt taccatcact ctcaaggaaa
     1201 tgagcgacga cggaaagcgt tcctccaccg tagcgcgcta tacgctgggt tccgcggcgc
     1261 cctggctggt ggacgatccg gagaacaaga ccacggatga caccacctat atgcagacgg
     1321 atttctacgg acgggacacc cgcattctgg tgggcaccgg caacatcctt cctctgttca
     1381 aatccggtat ccgccagctt cgggaccatc gtcccctgct cctgcatccg gccatgcccg
     1441 cctccattga aatcaacaat cggggccagc gcatcgccct gaaacgcgag actcccggct
     1501 ctccctggaa aatcacgtat cccctttctc tggatacgga cccggccatg atggatgtgc
     1561 tgctgggcac gctccaaaag ctgaccgccg ttcgcgtgta caacccggag gaaacaagcg
     1621 taccggacat gacggatgat cagatcacct ccgtttccat caggaatttc acaggccgtc
     1681 tttcagaaga cggcaaatcc ctgcaaaacg agctggatgt cgtcgaaggc atgcagtttg
     1741 accgcggcta cctgtcgccc tacttcatca acaaccccga gaagcaagtt gccctgctgg
     1801 acaacccctt cgtgctgctg ttcgacaaga agatcagcaa catccgcgac ctgctgccca
     1861 cgctggaagc cgtggccaag gccggcc
//
LOCUS       DAOMSU010000370         1884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6641_length_1884_cov_2.773100, whole genome shotgun sequence.
ACCESSION   DAOMSU010000370 DAOMSU010000000
VERSION     DAOMSU010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1884
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6641_length_1884_cov_2.773100"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1350)
                     /locus_tag="PK077_10750"
     CDS             complement(<1..1350)
                     /locus_tag="PK077_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HRN24815.1"
                     /translation="MSAKTYRTVPVRNLSSDELLELSKKQKLSLSREDMEVVQQIFRE
                     IDRDPTDVELEVIAQTWSEHCKHRIFSASISHSVNGGAPETINSLFKTYIKKPSEKIM
                     ERKPGFVLSAFDDNAGFIALDDNLAVCLKAETHNHPSAIEPYAGANTGLGGVIRDILG
                     AGKGAKPIASLDVFCFGAPDTNPADITAPDVIHPLGIMRGVVRGVRDYGNRMGIPTVN
                     GAIQFDPTYIYNPLVFCGTAGVIPRGDILKEMRPGLKVIVIGGRTGRDGLKGATFSSA
                     ALDEASHEEDFTAVQIGNPIEEKKTLDFILEARERGLIVFITDCGAGGFSSAAGEMLS
                     VTGGEIFLDNAPLKEPGLISWEIFLSESQERMVMAVEEKDLPELRKLADTFQTELTVL
                     GHSDDTGILKVWHKGELVCCLDNSKLHDAPIKKLESVFTPGKALTGQLLPDKDLNKSL
                     "
     gene            complement(1372..>1884)
                     /locus_tag="PK077_10755"
     CDS             complement(1372..>1884)
                     /locus_tag="PK077_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24816.1"
                     /translation="PLPWEPVFPPAPIFHFTTFLSDMTLHFEVISRFQAAANADALLY
                     TPLNAGLTFRRSRVYTVEITGDGQKAREYLLSVLVDPISQECTEQNAPILEGALFTID
                     YGMKAGALDLEKEAILQNYRGHRNMGFTLDGLKITQRVYVFGQGDKETLAARFAKDVC
                     NPAIHNWTIA"
BASE COUNT          336 a          491 c          635 g          422 t
ORIGIN      
        1 cagggatttg ttcaggtcct tgtccgggag gagctggccc gtgagcgcct tgccaggggt
       61 gaatacggat tccagcttct tgatgggggc gtcatgcagc ttggagttgt ccaggcagca
      121 gacgagttcc cccttgtgcc agaccttcag gattcccgtg tcgtcggaat ggcccaggac
      181 ggtcagttcc gtctggaaag tgtctgccag cttccggagt tccggaaggt ctttttcctc
      241 cacggccatc accatgcgtt cctgggattc cgacaggaag atttcccagg agatgaggcc
      301 gggttccttg agaggggcgt tgtccaggaa aatttcgcct cctgtgacgg agagcatttc
      361 cccggcggcg gaagagaagc cgccggcgcc gcagtccgta atgaagacga tgagaccgcg
      421 ttcgcgcgct tccagaatga agtccagcgt tttcttctct tcaatcgggt tgccgatttg
      481 cacggcggtg aagtcttcct catgggaggc ttcatccaga gcggcggagg agaacgtggc
      541 tcccttgaga ccgtcccgac ccgtgcgccc gccaatgacg attaccttga ggccggggcg
      601 catttccttc aggatgtcgc cgcgcggaat cacgccggcg gtgccgcaga agacgagggg
      661 gttgtaaatg tacgtggggt caaactggat ggcgccgttg acggtgggaa tgcccatgcg
      721 gttgccgtag tcgcgcacgc cgcgcaccac gccgcgcatg atgcccaggg gatggatgac
      781 gtccggggcg gtgatgtctg ccggattcgt atccggagcg ccgaagcaga agacgtccag
      841 ggaggcgatg ggcttggctc ccttgcctgc gcccaggatg tcgcggataa cgccgcccag
      901 cccggtgttg gccccggcgt acggttcaat ggcggagggg tgattgtgcg tttccgcctt
      961 caggcagacg gccaggttgt cgtccagggc gatgaagccg gcattgtcgt caaacgcgct
     1021 gagcacgaag cccggcttgc gttccatgat cttctcggaa ggctttttga tgtaggtttt
     1081 gaacaggctg tttatcgtct ccggcgcgcc tccgttgacg ctgtggctga tggaggcgga
     1141 aaagatgcgg tgcttgcagt gttccgacca cgtctgggcg attacttcca gttccacgtc
     1201 agtggggtcg cggtcgattt cccggaaaat ctgctggacg acttccatgt cttcacggga
     1261 cagggaaagc ttctgctttt tgctgagttc aagcagttcg tcgctgctca ggttcctcac
     1321 gggaacggtt cggtaggttt tggcggacat gtggatggaa aagaaaaagg gttaggcgat
     1381 ggtccagttg tgaatggcgg ggttgcagac gtccttggca aagcgcgccg ccagggtttc
     1441 cttgtctccc tggccgaaga cgtacacgcg ctgggtgatt ttcaggccgt ccagagtaaa
     1501 gcccatattc cggtgtccgc ggtagttttg caggatggct tctttttcca ggtccagggc
     1561 tccggctttc atgccgtagt caattgtgaa cagggcgcct tccaggatgg gagcgttctg
     1621 ttccgtgcat tcctgggaaa tggggtccac cagtacactc aggaggtatt cgcgggcttt
     1681 ttgtccgtcc ccggtgattt ctacggtgta aacgcggctg cggcggaaag tgaggccggc
     1741 gttcaggggg gtatagagca gggcgtccgc attggcggcg gcctggaacc ggctgataac
     1801 ttcaaagtgg agggtcatat cgctcaagaa tgtggtaaaa tgaaaaatgg gggccggcgg
     1861 aaagaccggc tcccatggga gggg
//
LOCUS       DAOMSU010000371         1882 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6655_length_1882_cov_2.072250, whole genome shotgun sequence.
ACCESSION   DAOMSU010000371 DAOMSU010000000
VERSION     DAOMSU010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1882
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6655_length_1882_cov_2.072250"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PK077_10760"
     CDS             complement(<1..407)
                     /locus_tag="PK077_10760"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070569 - uridylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HRN24817.1"
                     /translation="MSTFTPFAEKMESAGVSAVAVNAFSRCYEALVSNHSGMIPETDI
                     LPADQVEDWQDITASTPAADKDLIARCVCIKLNGGLGTSMGLQKAKSLLKIKGEDTFL
                     DLIVRQVKHLRSISGTPVRLLLMNSFSTSADTLA"
     gene            complement(506..>827)
                     /locus_tag="PK077_10765"
     CDS             complement(506..>827)
                     /locus_tag="PK077_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase type 11"
                     /protein_id="HRN24818.1"
                     /translation="TALKRRGLPYTTRFYFPSAEEYGSVLEQAGFRPETMKEFDRPTP
                     LKDGPDGLRNWANQFFREDLKNLHEEQRIQIFKEIENSLKDELWDGSQWVADYRRIRV
                     TAAR"
     assembly_gap    828..837
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<838..1258)
                     /locus_tag="PK077_10770"
     CDS             complement(<838..1258)
                     /locus_tag="PK077_10770"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRN24819.1"
                     /translation="MDWNAEWYENKHGFVAEYGRELLSCVPENPEQSILDLGCRTGTL
                     THALLEKSASVTGLDSSPEMIATARRLYPGMDFRVLDARLMPWRNRFDIVFSNAAFHW
                     IPDQETLLNAVYRVLKPQGKLVCEFGARFNILRIREAF"
     gene            1414..>1882
                     /locus_tag="PK077_10775"
     CDS             1414..>1882
                     /locus_tag="PK077_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24820.1"
                     /translation="MKATALFLAASALAAVSAGAYDGYGYDPRPASSYNNTISGAAHI
                     GYDSRYAYKDVVASSVLNRSGVFNIGGELNLNLARDWKQEIGAEYMAFCDGLLSDKDA
                     FDASWKAVKELFPNLSFRGGYELNYGGLPGYLSKHMGKAPHSLAQSVTAGLADD"
BASE COUNT          366 a          511 c          557 g          438 t
ORIGIN      
        1 gccagcgtgt ccgcgctggt ggagaaagaa ttcatcagca gaaggcgcac cggcgtgccg
       61 gagatggagc gcagatgctt cacttgcctg acgatcaaat ccagaaacgt atcctctcct
      121 tttattttca gcaggctttt cgccttctgg aggcccatgc tcgtgccgag gccgccgttg
      181 agcttgatgc agacgcaccg ggcaatcagg tccttgtccg ccgcgggggt ggaggccgtg
      241 atgtcctgcc agtcttccac ctggtcggcg ggcaggatgt ccgtttccgg aatcatgccg
      301 gagtggttgg aaacaagggc ttcgtaacaa cgggagaaag cgttgacggc cacggctgaa
      361 acgccggcgg actccatctt ttccgcaaaa ggagtgaacg tactcattaa tggcttttgt
      421 acaaaatgcc cggaggcggc tcaagcatta tcagcgtatc cggaatgccc gcgcgttttc
      481 tttttcctgt ggggcctgct ccggtttatc tggctgccgt cacccggatg cgccgatagt
      541 ccgctaccca ttgggaaccg tcccataact catctttcag ggaattttct atttccttga
      601 aaatctggat gcgctgttct tcgtggaggt ttttcaagtc ttcccggaag aattggttgg
      661 cccagttgcg caggccgtca ggaccgtctt tcagcggcgt ggggcggtca aattccttca
      721 ttgtttccgg gcggaacccg gcttgttcca gcacgctgcc gtattcttcc gcggagggaa
      781 agtaaaaacg cgttgtgtac gggaggcccc tgcgtttcag ggctgtcnnn nnnnnnngga
      841 atgcttccct gatgcggaga atattgaagc gggctccgaa ttcgcagacc agtttcccct
      901 gcggcttcag gacgcggtaa acggcattta agagagtttc ctgatccggt atccagtgga
      961 aggccgcatt ggagaaaacg atgtcgaacc tgttgcgcca gggcatgagg cgggcgtcca
     1021 gcacgcggaa gtccatgccg ggatagagcc ggcgggctgt tgcaatcatt tccggagagg
     1081 agtccaggcc ggtgacggaa gccgattttt ccagcagggc gtgcgtcagc gtaccggttc
     1141 tgcagcccaa gtccagaatg gactgttctg gattttccgg tacgcaggag agcagctctc
     1201 tgccgtattc cgcgacaaaa ccgtgtttgt tttcatacca ttcagcattc cagtccatga
     1261 cctctccatc gtaggggaaa aagggatttt ttcaagaaag actctgtctt gttttcatgg
     1321 catttctccc ggaggcgctt tgtccatttc tccgcgccgt gccgggattc ttccttgcgt
     1381 tggcggccgg gaaatgatta agataaactt actatgaaag caacagccct gttcctggcg
     1441 gcttccgccc tggccgcggt ttccgccggc gcctatgacg gatacggtta tgacccccgg
     1501 cccgcgagtt cctataacaa taccatcagc ggagcggccc acatcggcta tgattcccgg
     1561 tatgcctata aggacgtggt ggcttcctcc gtgttgaacc gcagcggcgt gttcaatatc
     1621 ggcggagagc tgaacctcaa cctggccaga gactggaagc aggaaatcgg cgcggaatac
     1681 atggctttct gcgacgggct gctgagcgac aaggacgcct ttgacgccag ctggaaggct
     1741 gtcaaagagc tgttccccaa tctttccttc cggggcgggt atgagttgaa ttacggcgga
     1801 ctcccggggt atttgagcaa gcacatgggg aaggctcccc attcccttgc ccagtccgtc
     1861 acggcggggc tggcggatga cg
//
LOCUS       DAOMSU010000372         1879 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6675_length_1879_cov_2.260417, whole genome shotgun sequence.
ACCESSION   DAOMSU010000372 DAOMSU010000000
VERSION     DAOMSU010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6675_length_1879_cov_2.260417"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="PK077_10780"
     CDS             complement(<1..354)
                     /locus_tag="PK077_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24821.1"
                     /translation="MSIPDYRIFFRRCAAFFLAGVLAAGSASAQQAAPPAAAPEQAEE
                     AKAEQPQEQPDQSEMDTVIMALCHEITILKKNEEKRMAIRKTLVREADATYNYFDTRV
                     YEMSAVLYALDDQKIF"
     gene            complement(584..>1879)
                     /locus_tag="PK077_10785"
     CDS             complement(584..>1879)
                     /locus_tag="PK077_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRN24822.1"
                     /translation="HVRTRFAPSPTGYLHIGGARTALFNWLFARKMGGTFILRIEDTD
                     NARNTEEATRAIFTGMEWLGLDWDEGPMKGGDCGPYFQSQRNDIYDAYFKKLQDAGRV
                     YEDEGAWRFRFDRSKPVTFHDLICGNITIDYRDASNTPDMAIRRADGSYIFHFVNVVD
                     DIEMKMTHVIRGEDHIMNTPKHIQLFEAFGVTPPVFAHMPLILNQDGSKMSKRDVGAA
                     LGTYPEEGFLPEGVMNFLALLGWSPKDDTEIFSPQELIERFSLEAVNHSAAKFDITKC
                     RWVNQQHIIALPAEEFALRARPFCLKAGLPDSPILDAAIATVQTKVQTLAEVPDKIRF
                     FFDLGMDPEAVSKVQPEALELLFKLADRLEQEPEWDGHELIGVLKAFAKENGVKMGAV
                     MFPARVALTGLSGGPDLSSVFSLLGREETVRRIRSFSLD"
BASE COUNT          396 a          503 c          559 g          421 t
ORIGIN      
        1 aaatattttc tggtcgtcca gcgcgtaaag cacggcggac atttcatata ccctggtgtc
       61 aaaataatta taggtagcat ccgcttccct caccagcgtt ttccggatgg ccatcctttt
      121 ttcctcattc tttttcagaa tggtgatttc atggcacaga gccatgatta cggtatccat
      181 ttccgattgg tccggctgct cctgaggctg ttccgccttg gcttcctccg cctgttccgg
      241 agcggcggca ggcggagccg cctgctgtgc agatgcagaa cctgcggcca gaacccccgc
      301 caggaaaaag gcggcgcagc ggcggaaaaa gatccggtaa tcagggatgc tcatgaatgg
      361 tccctgttct aggggctaca ggcattacgg tcaaggttat atattctcct gctgacggag
      421 ggtgagcttc acagaaaaaa acttttttca acgtgaaccg tatggaaagg acagggcatc
      481 ttccggcatc atttcccgaa tccccggaag atgcccttcc gtaaacagga aacgcatcgg
      541 agaatttccg gtgcgtttga aaagatgaat actccggcgg gaatcagtcc agagaaaagg
      601 aacggattct ccttacggtt tcctcccggc ccagcaggga aaagacggag gaaaggtccg
      661 ggccgccgct taacccggtg agggccaccc gggccgggaa catcacggct cccattttta
      721 cgccgttttc cttggcaaag gctttcagca cgccaatgag ttcatgtcca tcccattccg
      781 gttcctgttc tagcctgtcc gccagtttga aaagcagttc cagggcttcc ggctgaactt
      841 tggagacggc ttccggatcc atgcccaggt caaaaaagaa acggatttta tccgggactt
      901 ccgccagagt ctgcaccttc gtttgcacgg tggcgatggc ggcgtccaga atcggggaat
      961 ccggcaggcc ggctttcaaa cagaaggggc gtgcccggag ggcgaattct tccgcaggca
     1021 gggcgatgat gtgctgctgg ttgacccagc ggcatttggt gatatcgaat ttggcggcgg
     1081 agtggttcac ggcttccaga gagaagcgtt cgatcagctc ctgaggggag aaaatttccg
     1141 tatcgtcctt gggagaccaa cccagcaggg ccagaaagtt catgacgcct tccggcagaa
     1201 agccttcttc cgggtaggtt cccagggcgg cgcccacgtc gcgcttggac attttggagc
     1261 cgtcctggtt caaaatcagc ggcatgtggg cgaaaacagg gggagtgacg ccgaaggcct
     1321 caaacagctg gatgtgcttg ggcgtgttca tgatgtggtc ttccccgcga atgacgtggg
     1381 tcatcttcat ctcaatatcg tccaccacgt tgacaaagtg gaagatgtag gaaccgtccg
     1441 cgcggcggat agccatgtcc ggggtgttgg aggcgtcccg gtagtcgatg gtgatgtttc
     1501 cgcaaatcag gtcatggaag gtgacgggtt tggagcggtc aaagcggaaa cgccaggctc
     1561 cttcatcctc gtacacgcgg ccggcgtcct ggagcttttt gaaataggcg tcgtaaatat
     1621 cgttgcgctg gctttggaaa taggggccgc aatcgccgcc cttcatgggc ccctcatccc
     1681 agtccagccc cagccattcc atgccggtga agattgcgcg ggtagcctcc tccgtattgc
     1741 gggcgttatc cgtatcttca atgcggagga tgaaggttcc ccccatcttg cgggcaaaca
     1801 gccagttgaa cagggcggtg cgggcgcccc cgatgtgaag gtagcccgtg ggggaaggtg
     1861 cgaatcttgt tctgacgtg
//
LOCUS       DAOMSU010000373         1878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6680_length_1878_cov_2.782776, whole genome shotgun sequence.
ACCESSION   DAOMSU010000373 DAOMSU010000000
VERSION     DAOMSU010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6680_length_1878_cov_2.782776"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PK077_10790"
     CDS             complement(<1..208)
                     /locus_tag="PK077_10790"
                     /inference="COORDINATES: protein motif:HMM:NF014542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRN24823.1"
                     /translation="MGVIVGDLATDNDAERLSRADIPVVQITTGTMCHLDARMIAEAM
                     KKMPLDDLDVLIIENVGNLVCPASY"
     gene            1036..>1878
                     /locus_tag="PK077_10795"
     CDS             1036..>1878
                     /locus_tag="PK077_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRN24824.1"
                     /translation="MEATELYLKKSGGELPTQLFRFTDQGHRDITIRPELTASLGRIA
                     AAYQREYTKPLKWFEIGSCFRYEKPQKGRLREFYQFNADILGEASAWADSELIALAID
                     CMRELGFTQNDFIVRVSDREAWIRFASEHGVQEQDIPAFLGIVDKFERDRPEECQRKL
                     DAFHISRGDLVAFIENPPDGASERYDILMKDLTARGLDGYVKLDLSVVRGLAYYTGLV
                     FEIFDTQRSLRAVAGGGRYDTLVGALSNNAVDMPATGFAMGDAVITHLIEQTPHARAL
                     KDAAL"
BASE COUNT          472 a          554 c          433 g          419 t
ORIGIN      
        1 cataggaagc cgggcaaacc agattgccca cattttcaat aatcaaaacg tcgagatcat
       61 ccagaggcat tttcttcatg gcctccgcaa tcatgcgggc gtccagatgg cacatggtgc
      121 ccgttgtaat ctgcaccacg ggaatatccg ctcggctcag gcgttccgca tcattatccg
      181 tcgccaaatc ccctacgata acccccatgc gcacacggcc gcggagccgt cttctgcaac
      241 agagaagtct ttcctgaccc cggagaagaa aaaacattga taaccaaaag gttttttgca
      301 gcaaaaaaac ctctgttgcg ctctgccagc cggtcattgg catccaacac cggaacatgg
      361 acgtccatgc cgtggccgtg actttcatgg gagagctccc ccccgcatcc acattcttta
      421 cacataatca atactgatat tagattccca aagagaaaaa aaactcattc gtttcacgtc
      481 atactcctat gaaatcaaac aaaactgccc cggttttctt tttagcgcgg gaggaaaaca
      541 tttttctata aagggaaaga tgcaatgctc catacgcaga aaaacttttg ggaacacctt
      601 cttatcattt ccccttgctg ttcaatattg ttgtttgaca tccgtttaca aatccgcagg
      661 cacttaaacc cgcatgcccc acagcccgcg caaaaaatcc ggataattaa aaaaaacaat
      721 aaagtcccca ataatcaatc ctttcccgcc cttcctcatt tcatgtaaaa ccggaataac
      781 cgtccccatc ctcctgcata gagtcattca tatggtttcc accgaaattc cttcatcata
      841 agcatgtgga cttgccaagg cacaggaatt ctgtaaactg tttcgtcctc agacaacatc
      901 cttttttcca ggttatgcca gacgctcgct ttcaaccact tcccggattc cgcgattttg
      961 caccggcgga ctgcgccgtg cgcaactatc ttttcaacgc atcgttacgg attcctggaa
     1021 tgggagggcc cgactgtgga agccacggag ctttatctta aaaagagcgg aggcgagctg
     1081 cccacccagc tcttccgctt cacggaccag gggcaccggg acatcaccat ccgcccggaa
     1141 ctcaccgcat ccctgggacg catcgcagcc gcctaccagc gcgaatacac caaaccgctc
     1201 aagtggtttg aaatcggttc atgcttccgt tatgaaaaac cccagaaagg gcgtctgcgc
     1261 gaattttatc aattcaatgc ggacattctg ggagaagcct ccgcgtgggc ggattccgaa
     1321 ctgatagccc tggccatcga ctgcatgagg gaactgggct ttacgcaaaa cgacttcatc
     1381 gtgcgcgtct ctgaccggga agcctggatc aggtttgctt cggaacacgg cgtgcaggaa
     1441 caggatattc ccgctttcct cggcattgtg gacaaatttg aacgtgaccg cccggaagaa
     1501 tgccagcgca agctggacgc cttccatatc agccgcggcg atctggtggc cttcattgaa
     1561 aacccgcccg acggagcgtc cgaacgctat gacatcctga tgaaagacct gacagcccgc
     1621 gggctggacg gctatgtcaa actggactta tccgtagtgc gcgggctggc ctactacacc
     1681 ggtctggttt ttgaaatctt tgacacacag cgcagcctgc gcgccgtagc cggcggagga
     1741 cgttacgaca cgctggtagg cgccctttcc aataacgcag tggacatgcc ggcaaccggc
     1801 tttgccatgg gggatgccgt catcacccac cttattgaac agacgcccca tgccagggcg
     1861 ctgaaagatg cggcgctg
//
LOCUS       DAOMSU010000374         1876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6691_length_1876_cov_2.437122, whole genome shotgun sequence.
ACCESSION   DAOMSU010000374 DAOMSU010000000
VERSION     DAOMSU010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6691_length_1876_cov_2.437122"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PK077_10800"
     CDS             complement(<1..373)
                     /locus_tag="PK077_10800"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HRN24825.1"
                     /translation="MLNFVYDNKTTLIFGKGTHHETGTLLKPLGKKILLHYGSGSIRR
                     SGLYDAVTASLKAAGVEYEELGGVQANPTLPLVYEGIRLCREHGLGLVLAVGGGSVID
                     SAKAIALGVPYEGDVWELYLSK"
     gene            867..941
                     /locus_tag="PK077_10805"
     tRNA            867..941
                     /locus_tag="PK077_10805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:900..902,aa:Arg,seq:cct)
     gene            1344..1826
                     /locus_tag="PK077_10810"
     CDS             1344..1826
                     /locus_tag="PK077_10810"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRN24826.1"
                     /translation="MMIGSYGGNLCICDWAKEKRRGTIDRRICRHLNAEYEEGTSEII
                     EQAVSQLREYFQGERRDFDIPIVFTGSEFQNSVWHELMKVPYGSTISYAELARRIHHP
                     RAVRAVASANATNPISIFVPCHRVIGSNQKLTGYGGGLDAKRELLALEARVSGSVLAL
                     "
BASE COUNT          436 a          439 c          540 g          461 t
ORIGIN      
        1 ttttggaaag gtatagttcc catacgtccc cttcgtaggg cacgcccagc gcaatcgctt
       61 tggcagagtc aatcacgctg cctcctccca cggccagcac gaggcccagg ccgtgttccc
      121 ggcagaggcg gatgccttca tacaccaggg gcagcgtagg gtttgcctgg acgcctccca
      181 gttcctcata ctccacgccg gcggctttta aggatgccgt gacggcatcg taaagaccgg
      241 aacgtctgat gctgccgcta ccgtaatgaa gcaggatttt tttgcctaac ggcttgagaa
      301 gggttcccgt ttcatggtgg gtgccttttc cgaaaataag agtggttttg ttatcgtaaa
      361 cgaagttgag catggcggga atgatacggc aacgctacgg tccttccgtc cccttctcaa
      421 gatttttgta tggcctgttc ccctttccag ggggggatgc tgttccggag gatattctct
      481 catgcagggg ttctgttctt ctctgttttt tcatcattgg ctcatctgga cggagctttc
      541 tggaggattg ttttctttcc gttgtttttt ggcgataaga ttcagcggaa atgactctct
      601 gcgggagtgg cggcgccttc cggccccgag gggccagcgg aagacgggta tttcattatt
      661 ctgggccgct tccggcaggt aatcggaaca atggccgtat tcggccaggg atgcgcggag
      721 aaaggagggg aaacgggaag tttcagaaat ccgtaaaaat tatcctggtg aatactgttc
      781 aaaatcacga tgcatggcat actcctcctg ctttttgctt gagcgcggag gccttcccgt
      841 ttattgtcat gcacgggcca tgtccggtcc ccgtagttca atggatagaa cgggtctctc
      901 ctaaagacta gatgcaggtt cgattcctgc cggggacgcc acccaatatt attcagggcc
      961 ttgcatcagc attaaaaacg cagggatttt gcctctcttg tgcgaggtat cgtcatgatt
     1021 gattgaatgg ggaatgattc aagggaaaag caggtatccg gattatcggt gtccggccgg
     1081 agagggcgaa taacttggga ttcatgtccg attggaaaca aaaagcaaat gcggaagaaa
     1141 gattgcagat agcaaggaaa tgtgctaaag gctgccggag aagagccttt ggggcggaac
     1201 attgtgcttc gggacatgaa caggcaggca tggagcaatg tttcccagcc gtacagggga
     1261 tggatgaaag agcgttatgc aataacaagg acaagaagat ggaggcaatt aaaataatcc
     1321 gttatctgtc gcccgttgga gaaatgatga ttggctcata tggcggcaat ctttgcattt
     1381 gcgactgggc aaaggaaaaa cgccgcggca cgatagaccg ccgcatttgc cggcatctca
     1441 acgctgaata tgaagaagga acttccgaga ttattgaaca ggcggtaagc cagctccgtg
     1501 aatatttcca gggggaacgc agagactttg atatacccat tgtatttacc ggatcggaat
     1561 ttcaaaactc agtctggcat gaactgatga aagttcctta cggttccacc atttcttatg
     1621 cagagctggc gcgccgcatt catcatccga gggctgtccg ggctgtagcg tccgctaatg
     1681 ccaccaaccc catctccatt ttcgtgccct gtcaccgcgt catcggcagc aatcagaaac
     1741 tgacaggtta tggcgggggg cttgatgcga aacgggaatt gcttgccctg gaggcgcggg
     1801 taagcggaag tgtgctggcc ttatgacccg tgatgtgagg aagacaggca aggacgtgtc
     1861 aggtaaggct gttttg
//
LOCUS       DAOMSU010000375         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6762_length_1865_cov_2.577348, whole genome shotgun sequence.
ACCESSION   DAOMSU010000375 DAOMSU010000000
VERSION     DAOMSU010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6762_length_1865_cov_2.577348"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            285..899
                     /locus_tag="PK077_10815"
     CDS             285..899
                     /locus_tag="PK077_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24827.1"
                     /translation="MRLTLPWPLRRFLLGALLCSVTMASAASGAEFVVDASQATDPSG
                     NVYRTLQELSSSGALSSGDTVILCNDDSSLTSALKVAVHFRSNDPEVSRTIDLAGLSS
                     SGLYDYSQFPTGEKLELNSIILCNANTGVFFISTKLTSISSEYGVVFDSNTSGYIYGG
                     AICNLIYPISVGAGAVFTGNYASSNSGNARGGVFITATIVVLSP"
     gene            962..>1865
                     /locus_tag="PK077_10820"
     CDS             962..>1865
                     /locus_tag="PK077_10820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24828.1"
                     /translation="MIYSDGDISFSDGATFINNYAKTSGGAITMGGKCDLLALTKDVL
                     FSGNMVGGVVIGQSDDGFPVVENGIANAIDMNGSLFSSLYTPNLTLAAEEGIVFSGEL
                     YQGEDTHLAASRYNNFCADTTLYGGVLLLEHDVVFGRNPEEIMDKRYNSSLTVEKGVL
                     EITGGSTANASSFTLSGPGAVLRPGESAFINTHNADLSHGFTFDMRYQLQAGPAFGPG
                     LMLSALETFTAGGYIGVADTEANAPWFYADRSWKQDRVFHVLTDVQHIHEGDFDGAVS
                     QATGTARVDDPYAYTGTWSHRWTDA"
BASE COUNT          403 a          509 c          494 g          459 t
ORIGIN      
        1 tagtaggaaa tatatttttt catgccgcca tggaaaccgt atgatttaca tgaacgaaaa
       61 tatattgatg ataaaataat tgtatttctt tttcatggct ctccggctac tggctgccgc
      121 tgttcttcgg gggaatgacg cgcgcctgcc gtttccggca ggaactattt tggaataagc
      181 gggatatttt ctaaaacttt ctctgtcaac gcgccattta aacagcggat tcgaaatccg
      241 ttacttagcg gccttcccaa tattaacaat tcttattaaa agatatgaga cttactcttc
      301 catggccgtt gcgccgtttt ctgctggggg cgcttctgtg ttccgtaacg atggcgagcg
      361 ccgcttccgg ggcggagttc gtggtggacg cctcccaggc aaccgacccg tccgggaacg
      421 tgtaccgcac gcttcaggaa ttgtcctctt ccggggcgct gtcttccggg gataccgtta
      481 tcctgtgcaa tgatgattct tctctgacct cagccctgaa ggtagctgtc catttccgtt
      541 ccaatgatcc ggaggtgtcc cgcaccattg acctggccgg gctgagttcg tcaggtttat
      601 atgattattc ccaatttccc acaggagaaa aattggaact gaattccatc atcctgtgta
      661 atgcgaacac aggagttttc tttatatcaa ccaaattaac gtcaatttct tctgagtacg
      721 gggtagtatt cgactccaat acttcggggt atatttatgg cggggctatt tgtaacttaa
      781 tataccctat cagcgttgga gctggagccg tgtttaccgg caactatgct tccagtaatt
      841 cagggaacgc caggggcggg gtatttatca cagcaacaat cgtggtactt tcaccatagg
      901 gcccaaccac agtttcatca aaaattttgt ttactccgga agtgctgatg cttatggcgg
      961 tgtgatttac agcgatgggg atatttcttt cagcgatgga gctacgttta tcaacaatta
     1021 tgccaagact tccgggggag ccatcacgat gggagggaaa tgcgacctgc tcgctctgac
     1081 gaaggacgtg cttttcagcg gcaacatggt tgggggcgtc gtcatcggcc agtccgacga
     1141 tggcttcccc gtggtggaaa acggcattgc gaatgccatt gacatgaacg gttctttgtt
     1201 ttcttcactt tatacgccca atctgacgct tgccgcggaa gaggggatcg tcttcagcgg
     1261 cgaactctac cagggagaag atacgcatct tgccgccagc cggtacaaca acttctgcgc
     1321 ggataccacc ctctatggcg gcgtcctcct gctggaacac gatgttgtct tcggcaggaa
     1381 tcctgaggaa ataatggata aacgatacaa ctcctccctg acggtggaaa agggtgtgct
     1441 ggaaatcacc ggaggaagca cggccaacgc ctccagcttc accctctccg gccctggcgc
     1501 cgtcctgcgc cccggggaga gcgccttcat caacacccac aacgccgacc tctcccacgg
     1561 cttcaccttc gatatgcgct accagctcca ggccgggccg gccttcggcc cgggcctgat
     1621 gctctccgct ctggaaacct tcacggccgg gggatacatc ggcgtggcgg acacggaagc
     1681 caacgctccc tggttctacg cggaccgctc ctggaagcag gaccgcgtct tccacgtgct
     1741 gacggatgtg cagcacatcc atgaggggga ctttgacggg gccgtctccc aggccaccgg
     1801 aacggcccgg gtggatgacc cctacgccta cacgggaacc tggagccacc ggtggacgga
     1861 cgcgg
//
LOCUS       DAOMSU010000376         1864 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6770_length_1864_cov_1.968491, whole genome shotgun sequence.
ACCESSION   DAOMSU010000376 DAOMSU010000000
VERSION     DAOMSU010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6770_length_1864_cov_1.968491"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /gene="dnaN"
                     /locus_tag="PK077_10825"
     CDS             <1..842
                     /gene="dnaN"
                     /locus_tag="PK077_10825"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRN24829.1"
                     /translation="VDVNQRNQATVECGRSQFKLNGLPADEFPELPSFEQATVYQVEQ
                     NMLRDCIRRTEYAISTDTTRYVLNGISLSFRNGKMTLVATDGRRLALAETALEFPEEQ
                     QLDAILPTKAVGELRRLLDEVGTVTIRFTRNQAAFEINDTLLISKLIDGNYPNYRQVI
                     PTDCKESIELPCGELLETVRRVSLLSVDKSTNIKLNFRENELEVASSAEGVGEAHESM
                     TITYSGRPLSISFNPDFFMAPLKTMAGDTIITLNLIDEMSPGVLKIGDEFLYVLMPMR
                     SPN"
     gene            complement(1147..1587)
                     /locus_tag="PK077_10830"
     CDS             complement(1147..1587)
                     /locus_tag="PK077_10830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24830.1"
                     /translation="MNTYYSYRSYRLGGRLLGTLGCGLAWAFFGSGWWSFLLGALLLF
                     LWLPVILGRPYFLTITSSSISWGNSISRKEIPWELIERIGCTPDHESEPGLMIELKDG
                     RILDVESSCFGSLPPVREAILQASGSYPFHLAPDLFADPGLLSE"
BASE COUNT          477 a          472 c          527 g          388 t
ORIGIN      
        1 aggtggacgt caaccagcgc aaccaggcga ctgtggaatg cggccgctcc cagtttaaac
       61 tgaatggcct tccggcggat gaatttccgg aactgccgtc ttttgagcag gctactgtgt
      121 accaggtgga gcagaatatg ttgcgtgact gtatccgccg gacggaatat gccatttcca
      181 cggatacgac ccgctacgtg ttgaacggca tttccctttc tttccgcaat ggtaaaatga
      241 ctttggtagc cacggacgga cgtcgtctgg ctttggcgga aaccgccctg gaatttccgg
      301 aagaacagca actggatgcc atcctgccca ctaaggctgt cggagaactc cgccgccttc
      361 tggatgaagt gggaaccgtc acgatccggt tcacacgcaa ccaggcagcc tttgaaatca
      421 acgatacgct cttaatcagc aaactgattg acggcaatta cccgaactac cggcaggtaa
      481 ttcctacgga ttgcaaggaa agtatcgaac ttccctgcgg ggaactgctg gaaacagtgc
      541 gccgtgtttc tctcctttcc gtggataaga gcacaaatat caagctgaat tttcgtgaaa
      601 acgaactgga agtggcttcc agcgcagaag gagtgggcga agctcatgaa tcgatgacga
      661 tcacttattc cggaaggccg ctttccattt cctttaatcc ggatttcttc atggctcccc
      721 tgaagacgat ggccggggat acgatcatta cgttgaacct cattgatgaa atgagcccgg
      781 gcgtattgaa gataggtgat gagttcctgt acgtgctcat gccaatgaga tctcccaact
      841 gattttgatg gtacaaaatc attcatacga gaacaggcgc cgtgctgtca cggcgcctgt
      901 tttttattga ttgaaaatga aagtgatgac tgggttattc acatgaatgt tctacgtgga
      961 acatttttca ttttccaccg tcgtcaataa accggacggt gatttccggg ggagggataa
     1021 aaaataggcg tgccggcatg gcaccagaaa aaacgcgtat aaacaccttc cggcgccggg
     1081 aacgtgcggt tcaggcgcaa gggaaggcac ggagtgccgc agcgggtcta aggaggctcc
     1141 gccgcgtcat tcgctcaaca accccggatc ggcgaacaaa tccggagcaa gatggaaagg
     1201 ataggaaccg gaggcttgca gaatggcttc ccgtacggga ggcagggaac cgaagcagga
     1261 ggattccaca tccagaatac ggccgtcttt cagttcaatc atcagtccgg gttcagattc
     1321 atgatcgggc gtacatccta tgcgctcaat cagttcccat ggaatttcct tgcgtgaaat
     1381 gctgttgccc caggagatgg aggaggaagt gattgtgaga aaataaggtc tccccagaat
     1441 gacgggaagc cacaggaaca agagaagagc gccgaggaga aagctccacc atccggaccc
     1501 gaagaatgcc caggccaggc cgcaccccag ggtgccgagg agtctccctc ccagccggta
     1561 gctgcggtag gaataataag tgttcatgga ttgcgaagtc aatgaaacgt caggccggcc
     1621 agggaacgcg aagcccccag ccgtactctg tacgatactc cttcattcct gcaaagacaa
     1681 gatgataagt caggaaatcc agtccagcat gtccggcggc agggaaccgc gggagcggat
     1741 cagggaaagg aaagcttcct ccatttccgg aggcggcgga aaaatgcccg gcatggcgcg
     1801 gcctgcggca agctccacgg cgcccaggtt atggagtgcc aggggagggt ctatgtagca
     1861 catt
//
LOCUS       DAOMSU010000377         1832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6954_length_1832_cov_2.307822, whole genome shotgun sequence.
ACCESSION   DAOMSU010000377 DAOMSU010000000
VERSION     DAOMSU010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6954_length_1832_cov_2.307822"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            60..404
                     /locus_tag="PK077_10835"
     CDS             60..404
                     /locus_tag="PK077_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HRN24831.1"
                     /translation="MPEFTNAFSGLKEDRKLTHDELVRAIRFMIAAEYEAIQLYTQLA
                     ESIDHKLAQEVLLDISNEEKEHAGEFLRLLQVLAPDEQGFYDEGMGETNEKIDKLKAP
                     CPDGACSSSAAH"
     gene            complement(422..937)
                     /locus_tag="PK077_10840"
     CDS             complement(422..937)
                     /locus_tag="PK077_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRN24832.1"
                     /translation="MIPSKEDRAARALDYFHQGYNCSQSVFAAFADVCGLTEETALKL
                     SSPLGAGIGRMREVCGAFCGLSMLAGSLYGNSSPETGEKEKIFSLVQSLAASFKQEFG
                     TLYCRELLGLSPERLAAETARPGERTAAYYASRPCERCIAFCARRAADLEAKARRHEN
                     TRPPLQGNRAL"
     gene            complement(953..1618)
                     /locus_tag="PK077_10845"
     CDS             complement(953..1618)
                     /locus_tag="PK077_10845"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HRN24833.1"
                     /translation="MISLWMSWVQDWGYAGVIVLMAMESSIFPVPSEVVIPPAAILSA
                     QEGASMSFWGVVAAGTFGSWLGSAITYAVARIVGRPVIMRWGKFFFMPPHKVEKAEIF
                     LQRYEVSGVFFARLLPVIRHLISIPAGIVRMGFGIFSLVTVLGSFAWCTVLAWYGHRI
                     GERHPELLKSPEELIRTVKSESLPLILAVLVLAVCYILMLRLTDRNKPEKAAADTSAE
                     KEQ"
BASE COUNT          430 a          482 c          557 g          363 t
ORIGIN      
        1 ctgaaaatat atgacaggaa cattttgaat gtattcattg cggtctgggt catatctgca
       61 tgcctgaatt tacaaatgca tttagcggcc taaaagagga tcggaaactc acccatgacg
      121 aattggtgag agccatccgg ttcatgattg cagcggaata tgaagctatc cagctttata
      181 cccagcttgc agaatccata gaccataaac tggcccagga agttctgctg gatatttcca
      241 atgaggaaaa ggaacacgcg ggcgaatttc tccgcctcct ccaggtgctg gccccggacg
      301 agcagggctt ttatgacgag gggatggggg aaaccaatga aaagattgat aagctgaagg
      361 cgccctgccc ggacggcgca tgcagcagtt ccgccgccca ttgacggcac cttgctcatg
      421 gttaaagcgc ccggttcccc tgcaaggggg gccgggtatt ttcgtgacgt cgggcttttg
      481 cttccagatc ggccgcgcgg cgggcgcaga aggcaatgca gcgttcacag gggcgggagg
      541 cgtaataggc ggcagtcctt tctccggggc gcgccgtttc cgcagccagg cgctccggag
      601 acagccccag cagttcccgg cagtaaagcg tgccgaattc ctgtttgaag gaggcggcca
      661 gggattggac cagggaaaag attttttcct tttctccggt ttccggggaa ctgtttccgt
      721 acaggctccc cgccagcatg ctcagtccgc aaaaagcgcc gcatacctcc cgcatgcgtc
      781 caatgcctgc ccccagggga gaggacagtt tcagggccgt ttcttccgtc aggccgcaga
      841 catctgcaaa agcagcaaaa acggattggg aacaattata tccctggtgg aaataatcca
      901 gcgcccgggc tgcccggtct tccttggaag ggatcatggg aaaaagggag ggttattgtt
      961 ctttttctgc ggacgtgtcc gctgcggctt tttccggctt gttgcggtcc gtcagacgaa
     1021 gcatgagtat gtaacagacg gccagaacca gcacggccag aataagggga aggctttcac
     1081 tcttgacggt acggatcagc tcctccggag atttgagcag ttccggatgc cgttcaccga
     1141 tgcggtgccc gtaccaggcg agtaccgtgc accaggcaaa cgagccgagc acggtgacca
     1201 gggagaaaat gccgaagccc atgcggacaa tgccggcggg aatggaaatg aggtggcgga
     1261 taacgggcag aaggcgggcg aagaagacac cggaaacttc gtaccgctga aggaagattt
     1321 ccgccttttc caccttgtgg ggcggcatga aaaagaattt gccccagcgc atgatgaccg
     1381 ggcggcccac gattcgggcc acggcgtagg taatggcaga acccagccag gagccgaacg
     1441 ttccggcagc caccactccc cagaagctca tggatgctcc ttcctgggcg gacaggatgg
     1501 ccgcgggcgg gatgaccact tcgctgggaa cgggaaagat ggagctttcc atggccatca
     1561 ggacgatgac acccgcatat ccccagtcct gcacccagga catccaaagt gagatcaact
     1621 catgcataac ggcgcgtatc ttgcattaaa atccttgaaa ggaaagtctt ttattgcgtc
     1681 ccgggaacgg ggctggtaag gtagagaggc gtccggcgtg cgccgatgca gatagtgatg
     1741 aagctttata tcatagcagg cgaaaagagc ggggacattc atggcgccct gctgttgaag
     1801 aatctgctgc gtgtgatgcc cggcatggag gt
//
LOCUS       DAOMSU010000378         1826 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_6989_length_1826_cov_1.488989, whole genome shotgun sequence.
ACCESSION   DAOMSU010000378 DAOMSU010000000
VERSION     DAOMSU010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1826)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6989_length_1826_cov_1.488989"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="PK077_10850"
     CDS             complement(<1..463)
                     /locus_tag="PK077_10850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRN24834.1"
                     /translation="MPPLFSIIIPVYRSGPFLRDCLDSIRNQTLTDWECICINDGSPD
                     GSGAILDEYARKDSRFTAIHQDNRGVSAARNAGLSVARGNWTAFVDGDDTVEPDMLAC
                     LHETALRTPESSLLCYGISKDFHTGFRLIRTETTLPSRDQHIPAGETGAFLR"
     gene            complement(488..1336)
                     /locus_tag="PK077_10855"
     CDS             complement(488..1336)
                     /locus_tag="PK077_10855"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRN24835.1"
                     /translation="MLDLSVIILARNEELHIRRCLENILPVAKEVFVIDCFSTDNTTA
                     ICREYPRVKVIQHEWPGLYALQFNWALDNCPITAEWVLRLDADEWFMPEALEELKEKL
                     PALSPDVTGIIHKRRHIFLGRWMKHGVYPVKLLRLFRYGAARCEQRHMDEHMELSRGR
                     SVEFEYDFVDENLNDLGWWAHKHVDYSAREVADIEDILSSSAAASGIDGQAGRKRAMK
                     ERYARQPLFWRSFAYFCYRYFLKLGFLDGREGFLWHFMQGWWYRTLVDARQFEKQKQG
                     SANTER"
     gene            complement(1340..>1826)
                     /locus_tag="PK077_10860"
     CDS             complement(1340..>1826)
                     /locus_tag="PK077_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative colanic acid biosynthesis
                     acetyltransferase"
                     /protein_id="HRN24836.1"
                     /translation="YCWYIVNRTLFRLMVTNAMKVPRNLLLRLFGANIPLASLVYPSS
                     KIWAPWNLSVGEYACIGPDTEIYNKAPISIGNHAVISQGAFLCTASHDISDPAHALIS
                     APITVEDQAWVAAQAFVGMGVTVGRGAVVGARSAVFRDVAPWTVVGGNPARFIKQRTL
                     R"
BASE COUNT          350 a          501 c          597 g          378 t
ORIGIN      
        1 agcgcaggaa agcccccgtt tcccccgcgg ggatgtgctg gtcccggctt gggagggtgg
       61 tctccgtgcg gatgagccgg aaccccgtgt ggaagtcttt gctgattccg tagcagagga
      121 gggaggattc cggcgtgcgc agagccgtct cgtgcaggca ggccagcatg tccggctcta
      181 cggtgtcatc cccgtccaca aaggcggtcc agtttcccct cgccacggag agtcccgcgt
      241 tgcgcgcggc gcttacgccc cggttgtcct gatggatggc ggtgaaacgg gagtccttcc
      301 gggcatattc gtccagaatg gctccgctcc cgtccgggga gccgtcattg atgcagatgc
      361 attcccagtc cgtcagggtc tggttcctga tggagtccag gcagtccctg aggaaggggc
      421 cggaacggta aacggggatg atgatggaaa aaaggggggg catggagata aagcggggta
      481 atggcggtca gcgttccgtg ttggcggagc cttgtttctg tttctcgaat tgccgggcat
      541 ccacgagggt cctgtaccac catccctgca tgaaatgcca taaaaatcct tccctgccgt
      601 ccagaaaacc cagtttcagg aagtaacggt agcagaagta ggcgaaggaa cgccagaaga
      661 gcggctggcg ggcgtagcgt tccttcatgg cgcgttttct gcctgcctgg ccgtcgatgc
      721 cggaggccgc cgcggaggag gagaggatgt cttcaatatc cgccacttcc cgggcggaat
      781 agtccacgtg tttgtgcgcc caccatccca ggtcattcag gttttcgtcc acgaagtcat
      841 attcgaattc aacggagcgg ccccggctga gttccatgtg ttcgtccata tggcgctgtt
      901 cgcacctggc cgcgccgtag cggaacagcc gcagcagttt gaccgggtaa acgccgtgct
      961 tcatccaccg gcccaggaaa atatgcctgc gcttatggat gattccggtg acgtccgggg
     1021 aaagggcggg cagtttttcc ttcagctctt ccagggcttc cggcatgaac cattcgtccg
     1081 cgtccagcct cagcacccat tctgcggtga tggggcagtt gtccagagcc cagttgaact
     1141 ggagggcgta caggccgggc cattcatgct ggatgacctt caccctgggg tattcccggc
     1201 agatggcggt ggtgttgtct gtggaaaaac agtcaatgac aaagacttct ttagcgacgg
     1261 gcagaatgtt ttccagacag cggcggatat ggagttcctc atttctggcg aggatgataa
     1321 cggagaggtc aagcatggtt tagcggagcg tgcgttgttt gatgaatcgg gccgggtttc
     1381 cgcccacaac cgtccaggga gccacatccc tgaatacggc gctgcgggct cctaccacgg
     1441 ctccccggcc aaccgtcacc cccatgccca caaaggcctg cgccgccacc catgcctggt
     1501 cttccacggt aatgggggca gagataaggg cgtgggcggg gtccgagatg tcatgggatg
     1561 ccgtgcagag gaaggccccc tgggaaatga cggcgtggtt tccgatggag atgggggcct
     1621 tgttgtagat ttccgtatca gggccgatgc aggcgtattc ccctacggag agattccatg
     1681 gcgcccagat tttactgctg gggtaaacga gggaggcaag cggtatgttt gccccgaaca
     1741 ggcggagcag caggttccgg gggactttca tggcgttggt gaccatgagc cggaagagag
     1801 tccggttgac gatgtaccag caatac
//
LOCUS       DAOMSU010000379         1808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7080_length_1808_cov_2.435824, whole genome shotgun sequence.
ACCESSION   DAOMSU010000379 DAOMSU010000000
VERSION     DAOMSU010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7080_length_1808_cov_2.435824"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="PK077_10865"
     CDS             <1..241
                     /locus_tag="PK077_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24837.1"
                     /translation="ARRHPNIAYSMFSRPKGIQVTLNGTYTFDATLRDDWTPQIVVPP
                     YYRQPVHTIKIRINSIYRGTGTSDTYIGILKPIIQ"
     gene            complement(335..1054)
                     /locus_tag="PK077_10870"
     CDS             complement(335..1054)
                     /locus_tag="PK077_10870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HRN24838.1"
                     /translation="MNGVPPLEFAGVSHWAALAALLAAGACILELGQSYKKTVRNRTT
                     FWLGAACLFSLVPDLAAMLADEPEKNWTWMLPLHFCSVMQVVCALSLWIPSRRLRSVA
                     YYCVLCATLQGLVTPSVAHDFPSWTYFAFFLSHGVTVITALYLPLALEWKPARWDFLW
                     AFLLANAYLVAIHPVNMLLGTNYGFTVATPAGGSVLDFLGPWPWYLLWMQVPALALMY
                     LLTLPFRRYPRGRTGSSLLRH"
     gene            1170..1667
                     /locus_tag="PK077_10875"
     CDS             1170..1667
                     /locus_tag="PK077_10875"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRN24839.1"
                     /translation="MFILVCGLLAAGLTGCDDSSERRARYAEKKPAPRPLERTFDPPK
                     KVLPPAKPKKKAPVWMDYKGEDMRQLTELSGRKVLLVFYASWSRPAMDYVQAVKSYAE
                     SQDGKAFAVLIDADAYPDIARKYGLEAVPLTVLYLEGMKLKDMVGGITAPRLNELIEQ
                     TIRVQ"
BASE COUNT          415 a          499 c          559 g          335 t
ORIGIN      
        1 agcccggagg cacccgaata ttgcctactc catgttcagc cgccccaaag gcattcaggt
       61 gacgctgaac ggaacttata cttttgacgc cacgctgcgg gatgactgga ctccgcagat
      121 cgtcgtgcct ccttattaca ggcagcctgt gcacaccatc aaaatcagga ttaattccat
      181 ttatcgcgga acagggacat cggacaccta catcggcatc ctcaagccga ttatacaata
      241 aagaactttc cggggctctt tttttccgga aacgggcggg ccgttttgcg gggattccgt
      301 cggaagcggc ggggcgggga agaaagattc cctttcaatg gcggagcagg gaactgcccg
      361 tgcggcccct gggataacgg cggaagggaa gcgtgagcag gtacatcagc gccagcgcag
      421 gaacctgcat ccacagcagg taccagggcc atggcccgag aaagtccagc acggaacctc
      481 cggcgggcgt ggccacggta aacccgtaat tggttcccag gagcatgttg acgggatgaa
      541 tggccaccag gtacgcattc gccagcaaaa aagcccacag gaaatcccag cgcgccggct
      601 tccattccag agccagaggc aggtaaagcg ccgtgatgac tgtcacgccg tgggacagga
      661 aaaaggcaaa atacgtccat gaggggaaat catgggccac ggaaggagtc accagcccct
      721 ggagggtggc gcacagcacg cagtaatagg ccacggagcg cagccgccgg gagggtatcc
      781 acaggctcag ggcgcacacc acctgcatga cggagcagaa atgcaggggg agcatccatg
      841 tccagttctt ttccggttca tcggccagca tggcggccag gtcgggaacc aggctgaaca
      901 ggcaggccgc ccccagccag aaggtagtgc ggtttctgac ggttttcttg taggactgcc
      961 ccagctccag gatgcaggct cccgccgcca ggagtgcggc cagggcagcc cagtgggaaa
     1021 cacctgcgaa ttccagtgga gggacgccat tcatgcaacc ttcaccataa cagaaagagg
     1081 ggggaggggg caacgcagaa aagcgggatg ggtttcaggc ttgcaaattc gccttctccg
     1141 ggcatacttg cctgcggatg aaacagggct tgttcatatt ggtctgcggt ttgctggcgg
     1201 ccggactgac cgggtgcgac gattccagtg aacgtcgcgc ccgttatgcg gaaaaaaagc
     1261 ccgctccgcg ccctctggaa aggacgtttg accctcccaa aaaagtcctc ccgcccgcaa
     1321 aacccaagaa aaaggccccc gtatggatgg attacaaggg agaggacatg cggcagctta
     1381 cggaactttc cggccgcaag gtgcttcttg ttttttacgc ttcgtggagc cgcccggcca
     1441 tggattacgt ccaggcggta aaatcttatg ccgaatcgca ggacggcaag gcgtttgccg
     1501 tgctgataga tgcggacgcc tatccggaca tagctcgcaa atatggcctg gaagccgtcc
     1561 ctctgacggt tctgtatctg gaagggatga agctgaagga tatggttggc ggcattactg
     1621 cgccgcgcct gaacgaactg attgagcaga cgatacgggt gcaatagaag ggatgggctg
     1681 cggcagctac tgcgcggtgc gggggcggtt gatcccctgt ggatgaacct tctgtttgtt
     1741 gtgctttttt gctccgtacc tgaaaggact tccctttccc ccggagaatt ccgttccagg
     1801 aggcatgg
//
LOCUS       DAOMSU010000380         1789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7222_length_1789_cov_2.891580, whole genome shotgun sequence.
ACCESSION   DAOMSU010000380 DAOMSU010000000
VERSION     DAOMSU010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7222_length_1789_cov_2.891580"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1789)
                     /locus_tag="PK077_10880"
     CDS             complement(<1..>1789)
                     /locus_tag="PK077_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24840.1"
                     /translation="LVLAFAVGRREFGMGAFDLTQQGRYIDAQGKLNDLRSSGVMPPP
                     ADLYCAGKPPVNWYGNAPRTVDEECYKKVEAYLDRKKITPKQAWLRKYPTVSEIGDSA
                     ITEDNLAGFLHEYMYRHGQLRRKRDPFPTPVEFFSYLVDKYEHCGDLRDVDRKRVEWT
                     GVSLEGTPWPAMMALSETRPLRECDSVWERYMGQRGPTRLWLYGPYRADDDKEPPILF
                     SFDPDPEWSRESNERKLHEGGVCGTMSLISRNSQIARGIPAAPAGQPGHGNLMTTHFN
                     GNGCWLSVGQSVDTLKATTGFWYFRDSNAPRTGNAEYQSGLALSMNIDYEKFIDSRFA
                     MNIYKLAAIGSTTEETAAPSVTLPKEFTQTAMRTVLKANPFYTEAWYTLFKQEPQDLM
                     GATKMVDEVREALPDGMGIRKLWKTRKYVPSVGRGDKNGKEMLASHAKEYVNVLCSVI
                     LENALKQEYDYKTFQWAELMSWLKSESKRNSYPEPQAAYQIAYAKAQGADRLKRTVDR
                     GFKKALNFYRDDNNALKEPKDVDQEEMSFSLDALCQALPKEELVPWMKNMLDTCPDGF
                     KYKPKNKKETKIHPFYSALTKNYMSLADDS"
BASE COUNT          311 a          486 c          547 g          445 t
ORIGIN      
        1 gaatcgtcag ccagagacat gtagtttttc gtcagggcgc tgtagaatgg gtggattttc
       61 gtttccttct tgtttttggg cttgtatttg aacccgtccg gacaggtgtc cagcatgttt
      121 ttcatccagg ggaccagttc ttccttgggc agggcctggc acagggcgtc cagagaaaag
      181 gacatttcct cctgatccac atccttaggc tccttcaggg cgttgttgtc gtcccggtag
      241 aagttgaggg ctttcttgaa tcccctgtct acggttcttt tgagcctgtc tgcgccttgg
      301 gccttggcat acgctatctg atacgcggcc tgaggctccg ggtaggagtt gcgcttggat
      361 tcagacttga gccaggacat gagttcggcc cactggaagg tcttatagtc atattcctgt
      421 ttcaaggcat tttccagaat tacggagcag agcacattga cgtattcctt ggcgtggctg
      481 gcaagcattt ccttgccgtt cttgtcgcca cggcctacag agggaacgta tttgcgcgtt
      541 ttccagagtt ttctgatgcc catgccgtcc ggcagggcct cccttacttc atccaccatc
      601 ttggtggctc ccatgaggtc ctgaggttcc tgcttgaaga gcgtgtacca ggcttccgta
      661 taaaacgggt tggctttgag cacggtcctc atggcggtct gcgtgaattc cttggggagc
      721 gtgacggaag gggcggccgt ctcttccgtg gtggagccga tggccgccag tttgtaaatg
      781 ttcatggcga agcggctgtc gatgaacttt tcatagtcga tgttcatgga cagggccagt
      841 ccggactggt attccgcatt gccggtgcgc ggtgcgttgg aatcccggaa gtaccagaat
      901 cccgtggtgg ctttcagggt gtccacgctt tgccccacgc tcagccagca gccgttaccg
      961 ttgaaatggg tggtcatcag attaccgtgg ccgggctgcc cggcgggggc ggcgggaatg
     1021 ccgcgcgcaa tctgggaatt gcgggagata agggacatgg tgccgcacac accgccttca
     1081 tgaagcttgc gttcgttgga ttccctggac cattccggat ccgggtcaaa gctgaacagg
     1141 atgggcggtt ccttgtcgtc atccgcccgg taggggccgt acagccacag gcgggtcggg
     1201 ccccgctggc ccatgtagcg ttcccacacg ctgtcgcact cacgcagagg tcgcgtttcc
     1261 gaaagggcca tcatggccgg ccagggcgtt ccttccaggg aaacgccggt ccattccaca
     1321 cgcttgcggt ccacgtcgcg caaatcaccg caatgttcat atttatccac aaggtaggaa
     1381 aagaattcca ccggagtggg gaaggggtcc cttttgcgcc tcagctggcc gtggcggtac
     1441 atgtattcgt gcagaaagcc ggccaggttg tcttccgtaa tggcggagtc cccgatttcc
     1501 gagacggtgg ggtatttcct gagccaggcc tgctttgggg tgattttttt gcggtccaga
     1561 taggcttcca cctttttgta gcattcctca tccacggtgc ggggggcgtt cccataccag
     1621 ttgacgggcg gttttccggc gcaatacagg tccgctggcg gaggcatgac tccggaggat
     1681 ctcaggtcat tcagcttgcc ctgggcatca atgtagcggc cctgctgggt aaggtcgaag
     1741 gcccccatgc cgaattcgcg gcgtcctact gcgaacgcca gaacgagct
//
LOCUS       DAOMSU010000381         1788 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7227_length_1788_cov_2.163878, whole genome shotgun sequence.
ACCESSION   DAOMSU010000381 DAOMSU010000000
VERSION     DAOMSU010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7227_length_1788_cov_2.163878"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /gene="nagB"
                     /locus_tag="PK077_10885"
     CDS             complement(<1..736)
                     /gene="nagB"
                     /locus_tag="PK077_10885"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HRN24841.1"
                     /translation="MKVETFETPQDAAKALAGEVAELIRTRAAEGKNVVLGLATGATP
                     LPFYAELVRMHKEEGLSFANVISFNLDEYSGLDRDHPESYWYFMHTNLFNHIDIKPEN
                     INLPSGTVKDDEIAAHCAAYEQKIKDCGGIDLQILGIGRTGHIGFNEPGSDDTTITRQ
                     VHLDELTRSDAAPAFGGIENVPTTAITMGVATIMGAREVALMAWGEKKASIVKKAVQG
                     PVTVEVAASYLQKHPNAKFLLDKGAAS"
     gene            922..>1788
                     /locus_tag="PK077_10890"
     CDS             922..>1788
                     /locus_tag="PK077_10890"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRN24842.1"
                     /translation="MAGLIISPRARIFQGHDWVYGTEVRKVFGNPQPGDVVALKDFKD
                     RFLGSAMFNPHSQIVARRFSRRKQELNGDFFSRRISQAVELRRRLLPEETLTRLVWSE
                     SDGLPGLIVDRYADYLVVQTLTIAMERRLPIILNVLEDLLSPRGIIVRNDSPMLAAEG
                     IAPSVRVARGQQPEPFAARSGSVQFMIDLQTGQKTGLYLDQLDNYAAVARAARGRRVL
                     DCFCNQGGFALACALAGASEVTAVDVSQDAMDAVARNARLNGVSVQCVTDNAFDFLKK
                     EAALVRDGGEHKW"
BASE COUNT          365 a          528 c          508 g          387 t
ORIGIN      
        1 gggaagcagc tcccttgtcc agaaggaact tggcgttcgg atgcttctga aggtaagagg
       61 cggccacctc caccgtcaca ggcccctgaa cagccttttt cacgatggac gccttctttt
      121 caccccaagc catcagagcc acttcacgag cgcccatgat ggtagccacg cccatggtaa
      181 tggcggtggt gggaacattt tcaataccgc cgaaagcagg agcggcgtcg gaacgggtca
      241 gttcgtccag atgaacctgc cgggtaatgg tggtgtcatc agagccgggt tcgttaaagc
      301 cgatatggcc agtccggcca atgccgagaa tctggaggtc aataccgccg cagtccttga
      361 tcttttgttc ataggcggcg cagtgggcgg caatttcatc atccttcacc gtgccggagg
      421 gcagattgat gttttccggc ttgatgtcga tgtggttgaa aaggttggtg tgcatgaaat
      481 accagtagga ctccggatgg tcacgatcca ggccgctgta ttcgtccaga ttgaaggaaa
      541 tgacgttggc aaaggaaagg ccttcctctt tatgcatgcg aaccagttcc gcgtaaaaag
      601 gcagcggagt ggcgcccgtg gcaaggccga gaaccacgtt tttgccttca gcggcgcgcg
      661 tacggatcaa ttccgcaact tctcctgcca aagccttggc agcatcctgg ggagtttcaa
      721 aagtttctac tttcataacg cgccctattt tactgcgttt ccgggcattt tcaaccgaaa
      781 tagtcccgga atgatgcaca gcggcgcact tgcgcaaaaa atccccgggt acggaggcct
      841 ccaccctttc cccatccgtt caggaaaccg cccgcattac ccttgaactt tcccccccga
      901 tcatgtattg cctttcctga catggcaggt ttgattattt ctccaagagc acgcattttt
      961 caaggacacg actgggttta cggcacggaa gtgcgcaagg tcttcggcaa cccgcagcca
     1021 ggggacgttg tggccctgaa ggatttcaag gaccgctttc tgggttccgc catgttcaat
     1081 ccccattccc agatcgtcgc ccggcgtttc tcccgccgca aacaggaact gaacggagat
     1141 ttcttttcca ggcgcatcag ccaggcagtg gaactgcgcc gccgtctgct tccggaagaa
     1201 actctcaccc ggcttgtctg gagcgaatcc gacgggcttc ccggcctcat tgtggaccgt
     1261 tacgcggatt atctggtcgt ccagacgctg acgatcgcca tggaacgccg cctccccatc
     1321 atcctgaatg ttctggaaga ccttctttct ccccgcggga ttattgtcag gaatgattcc
     1381 cccatgctgg cggcggaggg cattgccccc tccgtccggg tggcgcgggg gcagcaaccg
     1441 gaaccttttg ccgcacgcag cggcagcgtg caattcatga ttgacctgca gacggggcaa
     1501 aaaaccggcc tgtatctgga ccagcttgac aattacgccg ccgtggcgcg cgccgcccgc
     1561 ggacgacgcg tgttggactg cttctgcaac cagggcggtt tcgccctggc ctgcgccctg
     1621 gccggagctt cggaagtaac ggccgtggac gtttcccagg acgctatgga cgccgtagcg
     1681 cggaacgccc gcctgaacgg agtctccgtg cagtgcgtca cggataatgc gttcgacttc
     1741 ctgaaaaagg aagcggccct cgtccgggac ggaggagaac acaaatgg
//
LOCUS       DAOMSU010000382         1773 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7325_length_1773_cov_2.806752, whole genome shotgun sequence.
ACCESSION   DAOMSU010000382 DAOMSU010000000
VERSION     DAOMSU010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7325_length_1773_cov_2.806752"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1405
                     /locus_tag="PK077_10895"
     CDS             <1..1405
                     /locus_tag="PK077_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRN24843.1"
                     /translation="PQEASEPKDETVLEIALGITPEMEQAIHTIRTAENANKTDTPEY
                     AEAIDTFNAANGYQLEPKAKKILKGLAFRESDFHRSAREMSGGWIMRAYLAKLLVLEP
                     DLLMLDEPTNHLDLLSLLWLQRYLKNYPGAILMISHDRDFMDELVESVYDIDNEELVE
                     YRGNYSDFLQQKDVRFEQLQAAYRNQQKEIAHIQEFIDRFRSINSKAGQVQSRIKQLE
                     KMKLIQKPVARRKVFKFNFPQPPRSTQKVIELEKVCQSYGDRRVYENLDLLVERGERT
                     VLVGPNGAGKSTLLKILAGIIPIDSGKRTPGTTTRIGYFSQARTENLNPENTVLEEIM
                     KCSAEIREEEARSILGSFLFRRLDVEKRVSVLSGGEKSRLSLVKFLVDPPNLLLMDEP
                     TTHLDLLSVEALVQALKHYEGTLVFISHDVHFIRSLAQKTLHVNRGTITAYAGGYDYY
                     LEKSGILDDEKDGITAE"
     gene            1583..>1773
                     /locus_tag="PK077_10900"
     CDS             1583..>1773
                     /locus_tag="PK077_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24844.1"
                     /translation="MEKRMEMLDNRCRQEGKRPFEARYYGSASLKDILENSDEFSDLV
                     EPDEDGAHPGALRENEEAG"
BASE COUNT          447 a          522 c          470 g          334 t
ORIGIN      
        1 tccccaggaa gccagcgaac cgaaggatga aaccgtcctg gaaatcgccc tgggcatcac
       61 gccggaaatg gaacaggcca tccacaccat ccggacggcg gaaaacgcca acaaaacgga
      121 tacgccggaa tacgcggaag ccattgacac cttcaacgcc gccaacggtt accagctgga
      181 acccaaagcc aagaaaattc tcaaagggct ggcctttcgg gaaagcgact tccaccgctc
      241 cgcccgggaa atgtccggcg gctggatcat gcgcgcgtac ctggccaaac tccttgtcct
      301 ggagccggac ctcctgatgc tggacgagcc taccaaccac ctggacctcc tgtccctgct
      361 gtggctccag cgatacctga aaaactaccc cggagccatc ctgatgattt cccacgaccg
      421 ggatttcatg gatgagctgg tggaaagcgt gtacgacatc gacaacgaag aactggtgga
      481 ataccgcggc aattactcgg acttcctcca acaaaaggac gtgcgcttcg agcagcttca
      541 ggcggcctac cgcaaccagc agaaggaaat agcgcacatt caggaattta ttgaccgttt
      601 ccgctccatc aattccaaag ccggccaggt ccagagccgc atcaagcagc tggaaaagat
      661 gaagctcatc caaaaaccgg tagcccgcag gaaagtattt aaattcaact tcccccagcc
      721 gccgcgcagc acccagaaag tcattgagct ggaaaaagtc tgccaatcct atggcgaccg
      781 cagggtttat gaaaacctgg acctgctggt ggaacgcggg gagcgcactg ttcttgtagg
      841 tccgaacggc gcgggcaaat ccactcttct gaaaatcctg gcgggcatca tccccattga
      901 ctccggaaag cgcactcccg gcactaccac gcgcatcggc tacttctccc aggcgcgaac
      961 ggaaaacctg aatccggaaa ataccgtgct ggaggaaatt atgaaatgca gcgctgaaat
     1021 ccgggaggaa gaagcgcgct ccatcctggg ctccttcctg ttccggaggc tggatgtgga
     1081 aaaacgcgtc agcgttcttt ccggcgggga gaaatcgcgg ctcagcctgg tcaaattcct
     1141 ggtggatccg cccaacctcc tgctgatgga cgagccgacc acccacctgg accttttgtc
     1201 cgtggaagcc ctggtgcagg ctctgaagca ttatgaggga acactggtct tcatctccca
     1261 cgacgtgcac ttcatccgtt ctctggcgca aaaaaccctg catgtgaacc gggggaccat
     1321 caccgcctat gcgggcggat atgactacta tctggaaaaa tcaggcattc tggacgatga
     1381 gaaagacggc atcacggcgg aataggggcc cttcttctgc ccccctttcc ttgccatgaa
     1441 tttcatcctg ccttaaaaga acaggatgaa ggcttttctt gttcctgtct tatattctgc
     1501 gcttctttcc gggaactctc ttgcatttgg tgaaacggcc gccggaacgg cttcctccgc
     1561 agcagcggct ccggcggaaa aaatggagaa acgaatggaa atgctggaca accgctgcag
     1621 acaggaaggc aaacgcccgt ttgaggcccg ttattacggt tccgcctcct taaaggacat
     1681 tttggaaaat tcggatgaat tcagcgacct ggtagaaccg gatgaagacg gggcgcaccc
     1741 cggtgcactc cgggaaaatg aagaggccgg aca
//
LOCUS       DAOMSU010000383         1770 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7348_length_1770_cov_2.593586, whole genome shotgun sequence.
ACCESSION   DAOMSU010000383 DAOMSU010000000
VERSION     DAOMSU010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7348_length_1770_cov_2.593586"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1754)
                     /locus_tag="PK077_10905"
     CDS             complement(<1..1754)
                     /locus_tag="PK077_10905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRN24845.1"
                     /translation="MEHITGLSLDYLGEKLHSTGHRYTEDELKGLLTRLLRILDYLHS
                     RHIYHRDIKPGNILLTEEGTPVLIDFGAARHALKLHAATVLTTQGYSSPEQALGKTNI
                     GPWSDLYSVGATFYALLTGNPPDRAEARLAEDEMQPLHSDPVLTRYYSRQFLLSLDKA
                     LAPQINCRYQTAGEWIHDICAISGEESSATIQVLPADLRHAHPEGMPTPGAGKNIGSR
                     KAATGTDGLHSASRRQSARALRRNRHMMSLLVGSACTILLGAGAFYLLDYMKKAPMGT
                     TINTIKEAIQQPAASSNVGEPEPPPRDLASIRIPELKITEDAALEPRSLTEFRLRLDD
                     TILTRSGLPPILPEKLRISCIYLQIIPPAANGEKLYLTIRDSNGVLVDRSINAVPSFT
                     VPGQSTSTFFFPALPELSTDRTYTYSFENAAHQAVPMQMACFKNETKRTEEAFPKIRF
                     IAAPPVPEPALLHDPAYKNLVAAIAAPSPENKNILDQANSMPGMLPALEQLSKAGYPI
                     AQQALGKTLMSPDCPGGSRPEEGVEWLYRSATGGCYEAMKELGILLMDIPSYFPRIPQ
                     QPPLASRDYAQAARFLRMA"
BASE COUNT          330 a          441 c          610 g          389 t
ORIGIN      
        1 gccattctca ggaaacgcgc tgcctgggcg taatcccggg aggcaagggg aggctgctgg
       61 ggaatgcggg gaaaatagga ggggatgtcc atgagcagga tgcccagctc tttcatggct
      121 tcatagcacc ctcctgtggc ggaacggtaa agccattcca cgccttcttc cggcctggaa
      181 ccgccgggac agtccggaga catgagggtt ttccccagtg cctgctgggc tatcgggtag
      241 ccggctttgg aaagttgctc cagggccggc agcatacccg gcatggaatt tgcctggtcg
      301 agaatatttt tgttctccgg ggagggcgcg gcgattgccg ccacaagatt tttgtaggcc
      361 gggtcatgga gcagggctgg ttccggcacg ggaggagcag cgatgaaacg gattttcggg
      421 aaggcttcct ccgtgcgttt cgtctcgttt ttgaaacatg ccatttgcat gggaacagcc
      481 tggtgggcgg cattttcaaa ggagtacgtg taggtgcggt ccgtggacag ttccggaagt
      541 gcgggaaaga aaaaggtgga ggtggattgc cccggcaccg tgaaggaggg gacggcattg
      601 atggagcggt ctaccaggac gccgttggag tcccggatgg tgaggtacag tttttccccg
      661 tttgctgccg ggggaatgat ttggagataa atgcaggaga tgcgcagttt ttccggcagg
      721 atgggaggaa gcccggaacg ggtcaggatg gtgtcgtcaa ggcgcaaacg gaactccgtc
      781 agcgaccgcg gttccagcgc cgcgtcttcc gttattttga gttccggaat gcggatggat
      841 gccagatccc gtggcggcgg ttccggttct cccacgttgg agcttgccgc gggctgctgg
      901 atggcttcct tgatggtatt gatggtagtg cccatagggg cttttttcat gtaatccagc
      961 agataaaatg ctcccgctcc cagcaggatg gtgcatgcgc ttcctaccag cagggacatc
     1021 atgtggcggt tgcggcgaag agcgcgtgcg gactgcctgc gtgaggcgga atgaagaccg
     1081 tccgttccgg tggcggcctt tctgctgccg atgtttttcc cggcgccggg agttggcatt
     1141 ccttccggat gggcatggcg gaggtctgcc gggaggactt gaatagtggc ggaggattct
     1201 tccccggaaa tggcgcaaat gtcgtgaatc cattcgccgg cagtctggta acggcaattg
     1261 atctgggggg ccagcgcctt gtccagggaa agcaggaact gccttgaata atagcgggtg
     1321 agcacgggat cgctgtgcag cggctgcatt tcatcttccg ccaggcgcgc ttccgcgcgg
     1381 tccggaggat ttcctgtaag cagggcgtaa aaagtggccc ctacggaata gagatcgctc
     1441 cacgggccga tattcgtttt gcccagcgcc tgttccgggg aggaataccc ctgggtggtc
     1501 aggacggtgg cggcatgaag tttcagggca tgccgtgccg ctccgaagtc gatcagcacg
     1561 ggcgtgcctt cctccgtcag caggatgttg ccgggtttga tatcccggtg gtaaatgtgc
     1621 cgggaatgga gatagtccag aatccggagc aggcgcgtca gcagcccctt gagttcgtct
     1681 tccgtatacc ggtgcccggt gctgtgaagt ttttccccca gataatccag ggaaaggccg
     1741 gtaatatgct ccatggcgaa gtaggccgtg
//
LOCUS       DAOMSU010000384         1746 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7494_length_1746_cov_2.567711, whole genome shotgun sequence.
ACCESSION   DAOMSU010000384 DAOMSU010000000
VERSION     DAOMSU010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7494_length_1746_cov_2.567711"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="PK077_10910"
     CDS             complement(<1..718)
                     /locus_tag="PK077_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24846.1"
                     /translation="MELNYGNILNIIIESESLESPDWIWKARVGGDFYPGHWVCGCKL
                     AGISGDYYNPDDIDNPLKVVALPPEEEPFYEYGGNPGEEDRRRFYVLKEPCCREPVER
                     SELFVHALVFRKIALNIARSCGKTEREISGTELIPECLIRLFDFPVTAYFAFAFDERL
                     VSRCLRVIRQAQPERPFLLLVMGKRWRTAAMEAAIVEKYKGIVVSIPDVMVTTKDGFA
                     WAYGYSWVWLREGMPVHARTG"
     gene            complement(708..>1746)
                     /locus_tag="PK077_10915"
     CDS             complement(708..>1746)
                     /locus_tag="PK077_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24847.1"
                     /translation="LKKETDRIYRIFHLLQTCSSGEATADLREYAESLGVSVNRDFSN
                     GIELAVLHFLDGNISLLRQIEELHSIKKRKSYRILPPMKDALPFPMITEKEREKEKEG
                     CRQYLSRILSEKGMTDVCVISCDRISSDYWFTIKRGHYQETVDEVDTSAKEIVSSIRT
                     PGNTDIVIYRGSSKCLYINLQVSARIQWMMRAYAVIAGRMLFGSDIWELRNRYTLSVF
                     NKKPIADIEVFSNIPGLRRVAVYKLVMSRPLHARKRTEKEVLMRGFTGTYKSLSELGD
                     EDGPLIVVPSGFHVDKAFFYFEFEEGDPKTVSLTPDSNGLELESEQYRLIDMYFEQAG
                     FDQLYNRIHGA"
BASE COUNT          452 a          500 c          377 g          417 t
ORIGIN      
        1 cccccgtgcg cgcatgaaca ggcatgccct ccctgagcca cacccaggaa tagccgtacg
       61 cccaggcaaa tccatctttc gtcgtcacca tgacgtcggg aatggaaaca acgatgcctt
      121 tgtacttctc aacaattgca gcctccatgg cagcagtccg ccaacgcttc cccatcacca
      181 agagaagaaa gggcctttcc ggctgtgcct gccggatgac gcgcaggcat ctggaaacca
      241 gccgctcatc aaaagcaaaa gcaaaatagg cggttacagg aaaatcaaaa agcctgatca
      301 ggcactccgg aatcagctcc gttccgctga tttccctctc cgtctttccg caagaacggg
      361 caatattcaa agcaatcttc ctgaacacca gggcatgcac gaaaagttcg ctcctctcaa
      421 caggctcccg gcagcagggt tccttcaata cataaaaacg cctgcggtct tcttccccgg
      481 ggttccctcc gtactcgtag aaaggctctt cttccggagg cagagccacc actttcaaag
      541 gattgtcgat atcgtcgggg ttgtaatagt caccggatat tccggcaagt ttacagccgc
      601 atacccagtg ccccggataa aaatccccgc ccaccctggc tttccatatc caatcgggag
      661 actccaggct ctcactttca ataatgatat tgaggatatt gccgtaatta agctccatga
      721 atcctgttgt acaactggtc aaaaccggcc tgttcaaaat acatatcaat gagcctgtat
      781 tgctcgcttt ccaattccag gccattggaa tcgggagtta aagaaacggt tttcgggtct
      841 ccttcttcaa actcaaaata aaaaaacgcc ttgtccacat ggaacccgct ggggacgaca
      901 atcagggggc cgtcttcatc gccaagctcg gaaaggcttt tgtaagtgcc tgtaaatccc
      961 ctcatcaaca cctctttctc cgtcctcttc cttgcatgga gaggcctgga catgacaagc
     1021 ttataaacag caacccttcg cagacccgga atattactga acacctcaat gtcggcgatg
     1081 ggcttcttat taaacacgct cagggtgtag cggttcctta attcccaaat atccgagccg
     1141 aacagcatgc ggccagcaat aacggcatag gcccgcatca tccattggat tctggcgctg
     1201 acctgtaaat tgatgtacag gcatttcgaa ctccccctgt agatgacaat gtccgtattc
     1261 cccggagtcc gaatgctgct gactatctct ttggccgacg tgtcaacctc atcaacggtc
     1321 tcctgataat gcccccgttt gatggtaaac cagtaatcag aggaaatccg gtcacagctg
     1381 ataacgcata cgtcagtcat tcccttctcc gacaaaatcc gggacagata ttgacggcag
     1441 ccttcttttt ccttctccct ttctttctcc gtaatcatgg ggaaaggaag cgcatctttc
     1501 atggggggaa gaatacgata cgatttcctc tttttaatgg aatgcaattc ctcaatctgc
     1561 ctgagcaagc tgatattgcc gtccagaaaa tgcagaacgg cgagttcaat tccattggaa
     1621 aaatctctgt tgacggaaac gcccaacgat tcggcgtatt cccgcaaatc cgcagtagct
     1681 tcaccggaac tgcacgtttg caaaaggtgg aaaatacggt aaatcctgtc cgtctccttt
     1741 ttaagg
//
LOCUS       DAOMSU010000385         1737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7559_length_1737_cov_2.635553, whole genome shotgun sequence.
ACCESSION   DAOMSU010000385 DAOMSU010000000
VERSION     DAOMSU010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7559_length_1737_cov_2.635553"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..>1737
                     /gene="hypF"
                     /locus_tag="PK077_10920"
     CDS             156..>1737
                     /gene="hypF"
                     /locus_tag="PK077_10920"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HRN24848.1"
                     /translation="MAITTIEQAAAALKFEIAGMVQGVGFRPHVYRLAVRHGLKGFVR
                     NTESGVEIHVEGESGAPERFWTALMDGLPEHARVYGVERTVCEPAGFEEFRIEESDST
                     PGGVPVMLPDLAPCPECLEEMRDPFSRRYHYPFTNCTHCGPRYSIIETMPYDRAGTSM
                     KGFRMCPECRREYQDVEDRRFHAQPIGCPSCGPSVKVLFSDGSELGFGHGFDTPAAQV
                     AWVLADGLIVALLGVGGFQLLADASSEAAVRRLRRLKERDAKPFAVMVSDEAAAERLC
                     RLSEEEKRLLASPAAPIVLARGRKDVDLAPSVCMFSRFVGIMLPSSPLHALLMDVWGR
                     PLVVTSGNLSGEPLCISVEEGLEKLGHVADVFLVHDRPVVRPVDDSVVRVTDGRAMMV
                     RRARGYAPRPVWRTSAAAPDVLALGAGLKNTICWLKRGVAVMSQHLGDLSSAASLNAF
                     ERTVEALGRTLDAVPQVIAVDAHPDGPTFALGRRLARKWNATVVPVQHHQAHVLACAV
                     ENETLFPALGIAWDGTGFG"
BASE COUNT          276 a          462 c          617 g          382 t
ORIGIN      
        1 ttttaaccgt gacgccgccc tggctgcact gaacaaggtg gctcatcatg cgcacgtgat
       61 ggaggtgtct tccaagaccg gcgaaggaat ggaggcctgg tgcgaggaga ttgtggaacg
      121 tgcgcgccgc gcccgggaag gcacgattca gcatcatggc catcaccacc attgaacagg
      181 ccgccgcggc attgaaattt gagatagccg gcatggttca gggggtcggc ttccgtcccc
      241 atgtgtaccg tttggcggta cggcacggat tgaaggggtt tgtgcgcaat acggagtccg
      301 gtgtggagat tcatgtggaa ggggagtccg gcgctccgga gcgtttctgg accgcgctga
      361 tggacggcct gccggagcat gcccgggttt atggagtgga gcgtaccgta tgcgagcctg
      421 ccggttttga ggaattccgc attgaggaaa gcgattccac ccccggaggc gttccggtga
      481 tgctgccgga tctggctccg tgcccggagt gcctggaaga aatgcgcgac ccgttttccc
      541 gccggtatca ttatccgttt accaactgta cgcactgcgg tccccgctat tccattattg
      601 agacaatgcc gtatgaccgc gcgggcacca gcatgaaggg gttccgaatg tgtccggagt
      661 gccggaggga gtaccaggat gtggaggaca ggcgttttca cgcacagcct atcggctgcc
      721 cgtcctgcgg cccttccgtg aaggtgctgt tttcagatgg ttccgaactg gggttcggcc
      781 atgggtttga tacccccgcg gcacaggtgg catgggtgct ggcggacggt ttgattgtgg
      841 ccctgctggg cgtgggaggg tttcaattgc tggcggatgc ttcttcggaa gctgccgtca
      901 ggcgtctccg gcggttgaag gagcgtgacg ccaagccttt tgccgtgatg gtgtcggatg
      961 aggctgcggc ggagaggctg tgccgcttgt cggaggagga aaagcgcctg ctggcttctc
     1021 cggccgcccc cattgtgctg gcccggggaa ggaaggatgt ggatctggct ccttccgtgt
     1081 gcatgttcag ccgttttgtg gggataatgc ttccgtcttc ccctcttcat gcgctgctga
     1141 tggatgtgtg gggaaggcct ctggtggtga ccagcgggaa cctgagcggc gagcccctgt
     1201 gcatctccgt ggaggagggg ctggagaaac tgggccatgt ggcggatgtg tttttggtgc
     1261 atgaccgtcc ggtagtgcgg cccgtggacg attccgtggt ccgcgttacg gacggcaggg
     1321 ccatgatggt tcgccgcgct cgcggatatg ccccgcggcc tgtgtggcgc acatctgcgg
     1381 cggctcccga cgtgctcgcc ctgggggccg gactgaagaa taccatctgc tggctgaaaa
     1441 ggggcgtggc tgtcatgagc cagcatctcg gtgacctgag cagtgcggct tctctgaacg
     1501 cttttgagcg gacggtggag gcgctgggca gaaccctgga tgccgttccg caggtaattg
     1561 ccgtggacgc ccatccggac ggccccactt ttgcccttgg ccggcgtctg gcgcggaagt
     1621 ggaatgcaac ggttgtccct gtgcagcatc atcaggctca tgtgctggcc tgcgccgtgg
     1681 aaaatgaaac gctgtttccc gctttgggca tcgcttggga tgggacgggg ttcggta
//
LOCUS       DAOMSU010000386         1729 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7613_length_1729_cov_2.836320, whole genome shotgun sequence.
ACCESSION   DAOMSU010000386 DAOMSU010000000
VERSION     DAOMSU010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7613_length_1729_cov_2.836320"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="PK077_10925"
     CDS             <1..435
                     /locus_tag="PK077_10925"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRN24849.1"
                     /translation="TCILTNDEHVHEDVLLLLQRFWERVGCHCIRMKAADHDSSVARI
                     SHIPHALSALCVHSALDGGDAKLLGLVSAGGFRDTTRVSMGEPSMWAEILAENAPAVL
                     ERLDEALSQLGQVRNWLAAGDKEALREWLKAAAESRAQALGL"
     gene            570..1103
                     /locus_tag="PK077_10930"
     CDS             570..1103
                     /locus_tag="PK077_10930"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HRN24850.1"
                     /translation="MNIPRHRRQGRGLHRAAAIGSLLVLGQSAYAETQPAAAEEGVQE
                     MPERVMTIRSRSLRAETVSSATLTNMKTEEVPQTVNVITRDLMDSKGSDSLVEALRMD
                     SSVNTGGDMLLSRTADQYTIRGFAGSDVQLGNMPLPRGMGYGMDTSLIENIEIVKGPI
                     GSISGGQTSTLGAYGRS"
     gene            1100..>1729
                     /locus_tag="PK077_10935"
     CDS             1100..>1729
                     /locus_tag="PK077_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24851.1"
                     /translation="MTAYARLPHHGQKYRATIDDTRYRGDETNGFALRTVVAAEYERP
                     FWLSNGANGGQKYTVSPIFRWQHDSRTKTVLTTSFQYQNSPTTMGIPVLGGHFVGPYD
                     AWYGSPSGRLNSKSLLAMLDFERKLEKIWTIRIGGGIGYSDVDYNVWGISSSAGRGTS
                     TADYYNQMIASGKAKYEAAWSDEWNINWNFYSNALAEFKTGQVKHEALMG"
BASE COUNT          368 a          465 c          532 g          364 t
ORIGIN      
        1 acctgcatcc tgacgaatga cgagcatgtg catgaagatg tgctgctgtt gctccagcgg
       61 ttctgggagc gtgtgggctg ccactgcatc cggatgaaag cggcggatca tgattcttcc
      121 gtagcgcgca tttcccacat acctcatgct ttgtccgcct tgtgcgtcca cagcgccctg
      181 gatggagggg atgcgaagct gctggggctg gtatccgccg gaggtttccg cgacaccacc
      241 cgcgtttcca tgggggaacc gtccatgtgg gctgaaattc tggcggaaaa tgcccctgct
      301 gttctggagc gtctggatga agccctgtcc cagctgggac aagtgaggaa ttggctggcc
      361 gcaggggata aggaagcatt gcgggaatgg ctgaaggccg cggcggagag ccgggcgcag
      421 gcgttgggct tgtgaaaggg catggccgtt ccgccgccag atacgctttt gcctgaagga
      481 aaaggaacgg tctgttatca aattgttaac aatcgggaat ggacggccct ttgttccgtc
      541 ctatacttga cgccatgcgt ttttccaaca tgaatatccc ccgccatcgc cgccaggggc
      601 gcggtttgca ccgtgccgcc gccattggca gcctgcttgt tctcggacag tctgcctatg
      661 ccgagacaca gcctgccgca gcagaggaag gcgtgcagga aatgccggaa cgcgtgatga
      721 ccatccgttc caggtccctg cgggcggaaa cggtcagttc cgccactctt acgaacatga
      781 agacggagga agttccgcaa acggtaaacg tcattacgag ggatctgatg gattccaaag
      841 ggtccgattc cctggtggaa gcgctgcgta tggattcttc cgtcaatacg ggcggagaca
      901 tgcttttatc ccgtacggcg gaccagtaca cgatccgcgg ctttgccggc agtgatgtcc
      961 agctcggcaa catgcctctt ccgcggggca tggggtacgg catggatacg tccctgatag
     1021 aaaatattga gattgtcaaa ggccccatcg gttccatttc cggcggccag accagtacgc
     1081 tcggggcata cggacggagt tgacggctta cgcccgcctt ccccatcacg ggcagaaata
     1141 ccgcgcaacg attgacgata cccggtatag gggggatgaa acgaatggat ttgccctgcg
     1201 cacggtggta gccgccgagt atgaacgtcc gttctggctg agcaacggcg ccaacggagg
     1261 ccagaagtac acggtctccc ccattttccg ctggcagcat gattcccgca ccaagacggt
     1321 gctgacgacc agtttccagt atcagaattc tccgacgacc atgggcatcc ctgtgctggg
     1381 cggccatttt gtggggccgt atgacgcctg gtacggttct ccgagcggaa gactcaattc
     1441 caaatccctg ctggcgatgc tggatttcga acgcaagctg gaaaagatat ggacgatccg
     1501 cattggcggc ggtattgggt acagcgatgt ggactataat gtctggggca tttcctcttc
     1561 cgctgggaga ggaaccagca cggcggatta ttacaaccag atgattgctt ccggaaaggc
     1621 caaatatgaa gccgcctgga gcgacgagtg gaatatcaac tggaattttt attccaacgc
     1681 actggcggaa tttaagaccg ggcaggtgaa gcatgaagcc ctgatgggc
//
LOCUS       DAOMSU010000387         1714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7721_length_1714_cov_1.122966, whole genome shotgun sequence.
ACCESSION   DAOMSU010000387 DAOMSU010000000
VERSION     DAOMSU010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7721_length_1714_cov_1.122966"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="PK077_10940"
     CDS             <1..516
                     /locus_tag="PK077_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24852.1"
                     /translation="RMRRIYDYTHPARGTFAQRPYDTEPVPALRKEEMPGNLLPLEES
                     SSQGVRRKDGSMKRVLYLNIPETGNYVFFLKTPVKKGAKAFVRLHDAHLLDAEYGYNP
                     GTEVSSSMGEGTTEGDAGQTGRKPIPLEKGWHPLMVETEGFSGLPTLFWQKEGGKKEA
                     IPNSALHAAVP"
     assembly_gap    598..682
                     /estimated_length=85
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<683..1488)
                     /locus_tag="PK077_10945"
     CDS             complement(<683..1488)
                     /locus_tag="PK077_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HRN24853.1"
                     /translation="MQHVPSMGFPPNQWLIGPALATFFLLAGYFSASGLGDQGAGTWV
                     ARRLMVLLRPYLFWCAAYWLAAGMPLAPDTLASVFGLGICPMLTPMWFLRDLMIFTLA
                     AFLLSRFRPALYAFGLFCLFLHRWDDSLAWPSPYMFGDFALGVMLASAAPGCLNRWGK
                     MPLAVHVSILLASAALVWAGCADTFLIPDGSFSGLIVLSLLSFGIIVKAVSPGWSERL
                     TLWASGSFFVYCSHIFVLIVLMGAESCFPSPWPAWAWWCLVPAVYMMARSV"
     gene            1480..>1714
                     /locus_tag="PK077_10950"
     CDS             1480..>1714
                     /locus_tag="PK077_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24854.1"
                     /translation="MLHDNHQRRQQPSNLHPRQTRVNFFTGRRGDGHDSPILDDCFKK
                     ALFFIDFIDEKRPYQAFTGIVLWEIPENDFFLIF"
BASE COUNT          466 a          446 c          445 g          272 t
ORIGIN      
        1 aggatgaggc gtatttatga ttatacccat ccggcgcgcg gtacctttgc ccaaaggcct
       61 tatgacacag agccagttcc ggccctcaga aaggaggaaa tgccaggcaa cctgcttccc
      121 ctggaggaat cgtcctccca gggcgttcgc cggaaggacg gcagcatgaa aagagttctc
      181 tatctgaata tcccggaaac agggaactat gttttcttcc tgaaaactcc ggtaaagaag
      241 ggggctaaag cattcgtcag gctgcacgac gctcatctgc tggatgcgga atacggctac
      301 aatcctggaa cggaagtgtc ttcctccatg ggggaaggga cgactgaagg agatgctggg
      361 caaacgggga ggaaaccaat ccctctggaa aagggctggc atcccctgat ggtggaaacg
      421 gaaggttttt ccggacttcc cacgcttttc tggcaaaagg aaggcggaaa gaaagaagct
      481 attccgaata gtgcccttca cgcggcggtt ccttaaacgc atggaactga aaacggagcg
      541 gacgtttcct ctttccggtg gttatttgcc tgcagccacc agaacgagag cgcgcggnnn
      601 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      661 nnnnnnnnnn nnnnnnnnnn nnacgctccg cgccatcatg taaaccgcag gcaccaggca
      721 ccaccacgcc caggcaggcc atggggaggg gaaacagctc tctgcgccca tcaggactat
      781 cagcacgaaa atgtgggaac aatatacgaa aaaactgccg gacgcccaca gggtcagcct
      841 ttccgaccag ccggggctga cggctttcac aataatcccg aagctgagta aggacagaac
      901 aatcaatccg gaaaaagagc cgtccggaat caggaaagtg tccgcacagc ctgcccatac
      961 aagggccgca gaagctaaga gaatagaaac atgcacggca aggggcattt tgccccagcg
     1021 gttcagacaa cccggcgcag cggacgccag cataacgccg agcgcaaaat ccccgaacat
     1081 gtacggactt ggccacgcca gggagtcatc ccaccggtgc agaaacaggc agaacaggcc
     1141 aaaggcgtaa agggctggac ggaaacggga gagcaaaaac gcggccaggg taaaaatcat
     1201 caaatctctc agaaaccaca tcggggtaag catgggacat attcccagcc caaacacgga
     1261 agccagcgta tcaggggcaa gaggcatgcc cgccgccagc caataggccg cacaccagaa
     1321 aagataagga cgcagcaaaa ccatcaggcg gcgggccacc cacgttcctg ccccctgatc
     1381 cccaaggccg gaagcggaaa aataccccgc cagcaggaaa aacgtcgcca gcgccggacc
     1441 aatcagccat tggttgggag gaaaccccat ggaaggaacg tgctgcatga taaccaccag
     1501 cgtcgccagc agccgagcaa tctccaccca aggcaaacga gggtgaattt ctttaccgga
     1561 cgcaggggag acggtcatga cagccccatt ctggatgatt gctttaaaaa agcactattt
     1621 tttattgatt ttatagacga aaagcggcct tatcaggcct tcacaggcat cgtattatgg
     1681 gaaatacctg aaaatgattt ttttctcata tttt
//
LOCUS       DAOMSU010000388         1701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7814_length_1701_cov_2.367558, whole genome shotgun sequence.
ACCESSION   DAOMSU010000388 DAOMSU010000000
VERSION     DAOMSU010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7814_length_1701_cov_2.367558"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="PK077_10955"
     CDS             complement(<1..382)
                     /locus_tag="PK077_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24855.1"
                     /translation="MTLPPKFLLVLSLFQISLGLGWILGYVLLPPTIFCYEQAAEHLL
                     AGEYRTVRPEGLNTENSLVLQPYDYFNKGQTAQLPVVSLHRESSLNSFASYPPNPMDY
                     TVLFRHLYEQGARNVYVMSPMTWEE"
     gene            complement(401..1339)
                     /locus_tag="PK077_10960"
     CDS             complement(401..1339)
                     /locus_tag="PK077_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HRN24856.1"
                     /translation="MKELSRLVHRSFHIIPRQEDFRPLALGASGRTIVRIHFGGRTCI
                     GIQWGNDRSDNDSFIPTARHLHTHGVNVPEIYDYEPLGPGCGAALVEDLGDANLLAFR
                     KEPWPSLRLRYIRAMEQLHLLHSCPFPEEFPLQPAFDEALYRWEQSYFAEHFLGSHLG
                     LETDSFLNHPALKEQAQFLAALPECPVHRDSQSQNVHIHAGKTWLIDFQGMRGGRPEY
                     DLASLVYDGYARLEPEQADELLREWENISAHSIDHRIFRACALQRIMQMLGAYANIGH
                     NQGKTWYLAQIPAGLGHLRKLLPGSTLAEPLAAMLV"
     gene            complement(1438..>1701)
                     /locus_tag="PK077_10965"
     CDS             complement(1438..>1701)
                     /locus_tag="PK077_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HRN24857.1"
                     /translation="HAYLHDIGRVNYVTATDEEAVDAFFKLSRYEGIIPALESSHAIA
                     YAMKWARENRGGAILVNCSGRGDKDVDYVVEHYGYGENHQFPA"
BASE COUNT          392 a          433 c          533 g          343 t
ORIGIN      
        1 tttcttccca ggtcatgggg gacatcacgt aaacattcct ggctccctgt tcatacaggt
       61 gtcggaacag cactgtgtaa tccatgggat tgggaggata ggaagcgaaa ctgttcagtg
      121 atgattcccg gtgcagggaa accacgggaa gctgggccgt ctgccctttg ttgaagtaat
      181 cgtagggctg gaggacgagg ctgttttccg tattcagccc ttccgggcga acggtccggt
      241 attccccagc cagcaaatgt tccgctgcct gttcataaca gaaaatggta ggaggaagaa
      301 gcacataccc cagaatccag cccagtccca gggaaatctg gaacagggac aggacaagca
      361 ggaatttggg gggaagcgtc atcagggggg ctgggaaaaa tcatactagc atagcagcca
      421 gcggttctgc aagcgtggag ccgggcagaa gcttccgcag gtgtcccagc ccggcgggaa
      481 tctgagccag ataccaggtt tttccctgat tgtggccgat attggcgtag gcgcccagca
      541 tctgcataat ccgctgcagg gcgcaggcac ggaaaatgcg gtgatctatg gagtgagcgg
      601 agatattttc ccactccctg agcaattcat ccgcctgttc cggttccaga cgggcgtagc
      661 cgtcatagac cagggaggcc agatcatatt ccggacggcc gccgcgcatg ccctgaaaat
      721 caatcagcca ggtttttccc gcgtggatat gcacgttctg ggactggctg tcccggtgga
      781 cggggcattc cggaagggcc gccagaaact gcgcctgctc tttcagggcg ggatggttca
      841 ggaaggaatc tgtttccagc cccaggtggg accccaggaa atgttcagca aaataggact
      901 gctcccagcg gtacaaggcc tcgtcaaaag cgggctggag ggggaattcc tccggaaacg
      961 ggcaggagtg aagcagatgg agttgttcca tagcccggat gtagcgcagg cgcagggaag
     1021 gccagggctc tttccggaaa gccagcaggt tggcgtctcc caggtcttcc accaaggcgg
     1081 ctccgcatcc gggaccgaga ggttcataat cgtaaatttc cggaacgttg acaccgtggg
     1141 tgtgcagatg ccgggctgtg gggataaaag aatcattgtc cgaacggtca tttccccact
     1201 ggatgccgat gcatgtgcgg ccgccaaaat ggatgcggac gatcgtacgg ccggaggctc
     1261 ccaaagccag gggacggaaa tcttcctgac gcggaataat gtgaaaactc cggtgaacca
     1321 gacgggacaa ttctttcatg gaaaggtggc gtggctgcgt taagagcctt gcgtaaggat
     1381 ggcgtatgaa ggatgacggt atccatcata cgccgaaaat gaggaaaggc aggacaatca
     1441 ggcggggaac tgatggtttt ccccatagcc gtaatgctcc acgacgtaat ccacatcctt
     1501 gtctccgcgg ccggagcagt ttaccagaat ggcgccgcct ctgttttccc gggcccactt
     1561 catggcatac gcgatagcat gggagctttc cagagcggga atgattcctt cgtaacggga
     1621 gagtttgaag aaggcgtcca cggcttcctc gtctgtggcg gtgacatagt taacgcggcc
     1681 gatgtcgtgc agataggcgt g
//
LOCUS       DAOMSU010000389         1688 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7900_length_1688_cov_2.132884, whole genome shotgun sequence.
ACCESSION   DAOMSU010000389 DAOMSU010000000
VERSION     DAOMSU010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7900_length_1688_cov_2.132884"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PK077_10970"
     CDS             <1..197
                     /locus_tag="PK077_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24858.1"
                     /translation="MEKEQQPKQVEISMDFTPEQLEMIRQAAALRGETPGEWCERAIK
                     ALTAVSVSMNDYHRLGGKGG"
     gene            complement(247..645)
                     /locus_tag="PK077_10975"
     CDS             complement(247..645)
                     /locus_tag="PK077_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24859.1"
                     /translation="MNTTCPNCNNNITCPDHYVGKPCICPHCQHSFTAIPTVISQTAP
                     NSKKGKDKASEQESALSLATGTANVCIFFAVLTFIGTLITGIRTYPVPEISRIPLAGF
                     GLIILLALLLSMGWLVAASIIKLLVCIVKK"
     gene            complement(799..1170)
                     /locus_tag="PK077_10980"
     CDS             complement(799..1170)
                     /locus_tag="PK077_10980"
                     /inference="COORDINATES: protein motif:HMM:NF027249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HRN24860.1"
                     /translation="MTPHYTINIEWSDEDASFIATVPQLPGCMADGPTEEAALAEAKR
                     AIQDWLETARAINRPIPLPPPTIESLARASALLNKSAIARLIGLEQRTLNARIKNRTP
                     LTPKEAERLQSALQDNNLALI"
     gene            complement(1167..1427)
                     /locus_tag="PK077_10985"
     CDS             complement(1167..1427)
                     /locus_tag="PK077_10985"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HRN24861.1"
                     /translation="MSSKKKLLEKIMNPDKTGNVTFEEATKLLSHLHFQFRQTGGSHM
                     VAWHEKIPEIINIQPGPNGKAKPYQLKQIRTIINQYNLHKTL"
BASE COUNT          402 a          316 c          514 g          456 t
ORIGIN      
        1 tgatggagaa agagcagcaa ccgaagcagg ttgagatcag tatggatttt acgccggagc
       61 aactggaaat gatccgtcag gctgcggcgt tgagggggga aactcccgga gaatggtgtg
      121 aacgggctat taaggcatta actgctgtgt ccgtatcaat gaatgattat cacaggttag
      181 gcgggaaggg aggataaaaa agccgtgcat ggtgagaggc acggcgggag agggtgaaca
      241 atgttgctat tttttgacga tgcaaacaag aagcttgatg atgctggcag caacaagcca
      301 ccccatagaa agaagaaggg caagcaggat aataaggcca aatcctgcca gaggtatgcg
      361 ggatatttcc ggtactggat aagtgcggat gccggtgatg agagttccga taaaggttag
      421 tactgcaaaa aagatgcaga cattagctgt tccggtggca agggaaaggg cagattcctg
      481 ttcagaggct ttatcctttc cctttttgga attgggggct gtctggctga tgacggtagg
      541 aatagccgtg aaactgtgct ggcagtgcgg gcaaatgcag ggcttgccga catagtgatc
      601 cgggcaggtg atattattgt tacagtttgg acaagtagtg ttcattggtg aaatatccta
      661 tatttagctt aattttgaag gtgcggacaa gtttttttat aatgttagaa ttgatgctgg
      721 ccaagcagag tgaaggggga aacataaaaa gcccatggcc ggagccatgg gcttgtggga
      781 aaaagctggt gtgacaagtc agatgagggc aagattgtta tcctgcaacg cactctgaag
      841 acgttccgct tcctttggag tgaggggcgt ccggtttttg atacgggcat tcagggtacg
      901 ttgttccaag ccaatgagcc gggctatggc cgatttattg agcagggcgg aggcgcgggc
      961 caggctttca atagtagggg gaggaagggg aatggggcgg ttgatggcgc gggccgtttc
     1021 cagccagtcc tgaatagcgc gcttggcttc cgccagggca gcttcttccg tagggccgtc
     1081 cgccatgcag ccgggaagct gggggacggt ggcgatgaaa gaggcatctt catcgctcca
     1141 ttcgatgttg atcgtgtagt gcggggtcat agcgttttat ggaggttgta ttggttgata
     1201 atggttctta tctgtttgag ctggtagggt ttggcttttc cattggggcc gggttggatg
     1261 ttaataatct ctgggatctt ttcatgccag gcgaccatat gggaacctcc agtttgcctg
     1321 aattgaaaat ggagatggga gagcaatttg gttgcttctt caaaagtgac gttccctgtt
     1381 ttgtcagggt tcatgatttt ttccagaagc tttttcttgc tgctcatgtt tcgatattac
     1441 cttttttaga aaatcaagca aggaaaatat ttctcaattt agaaatatga agattccaga
     1501 taggagtttg ttacggatga atgaggttgg atgactctct gaagctggcc ttttttctct
     1561 ttttgctcaa atagttcttg ccttttggct tccgacggtg tttactccct tgcgcagtcc
     1621 ctgcaaacct aatgtcgcgt tcgtctagcg gtccaggact cccgcctttc acgcgggcaa
     1681 cacgggtt
//
LOCUS       DAOMSU010000390         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7957_length_1679_cov_2.748153, whole genome shotgun sequence.
ACCESSION   DAOMSU010000390 DAOMSU010000000
VERSION     DAOMSU010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7957_length_1679_cov_2.748153"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1100
                     /locus_tag="PK077_10990"
     CDS             <1..1100
                     /locus_tag="PK077_10990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24862.1"
                     /translation="WDEKPDSIVKAAVGYELDRFLHKALGRQMSESARRSPLPPDWKD
                     LIIPASNIAGKTDQFPRADRFVGGPPQITTSAPSLGTVVENNDLFSPSPENRVSPPLF
                     IRWGGDIIPTLPLIAALNALDLKPGDVRIIPGDTLFLGGKRSIPLDKHGRIPLAANSS
                     PTILDTKEVIVPVMSGLRPPDTTAVRKLLSSADAVLVSEPSALSDTPDAQSLLAAQTV
                     RSIMGALAPAPAVLIPIAPAWVQWIIVLDVLLLSVWALRFQRRGRFITWSLCILFVPF
                     IAWYLFSAHDLWFPVMTPVTAILCVALACSLFPQLSPPSRDAEDEDEEDDGTPSSEGS
                     GAGHVLPPDNLYQEPNEVPIPHHSKRERPDS"
     gene            1097..1552
                     /locus_tag="PK077_10995"
     CDS             1097..1552
                     /locus_tag="PK077_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HRN24863.1"
                     /translation="MKQRKHILFICTGNTCRSPMAEGLFRKLSAHHPEWQAGSAGTAA
                     WPGQAASPETLHVLREHGVNLSSHESRPVTDELMKNATDVYAMTESHLAALLANFPEH
                     ADKVKLVTCYTGNRGIADPIGCGQTAYNNVARQLTTAIQAIIARMEQQE"
BASE COUNT          355 a          579 c          416 g          329 t
ORIGIN      
        1 cctgggacga aaagccggac agcatcgtca aagccgccgt ggggtatgaa ctggaccgct
       61 tccttcacaa ggctctgggc cgacaaatga gcgaatccgc ccgccgctcg ccccttccgc
      121 cggattggaa agacttgatt attcccgctt ccaacatcgc aggaaagacg gaccagttcc
      181 cgcgcgcgga caggttcgta gggggacctc cgcaaataac cacgtcagcc ccctccctgg
      241 gaaccgtcgt ggaaaataac gatctgtttt ccccatctcc ggaaaacagg gtctccccgc
      301 cgctttttat ccgctggggc ggggatatca ttcccactct tcctctgata gccgctctga
      361 atgccctgga cctgaaaccc ggcgatgtca gaatcattcc gggagacacc ctttttctgg
      421 gcggcaaacg ctccattcct ctggataaac acggccgcat ccccctggcg gcaaacagca
      481 gccccaccat tctggacacc aaggaagtaa tcgtccccgt catgtccggc ctgcgcccgc
      541 cggatactac tgccgtcaga aaactgcttt cctctgcgga tgcggtactc gtttccgaac
      601 catccgccct ttccgacacg ccggacgcgc aatcccttct ggcggcgcag actgtccgca
      661 gcatcatggg agcgcttgcc cctgcccctg ccgtcctgat tcccatagcc cccgcctggg
      721 tacaatggat catcgtgctg gacgtcctgc ttctgtcagt atgggctttg cgttttcaac
      781 gcagagggcg cttcatcacc tggagcctct gtatcctgtt cgttcccttc attgcatggt
      841 acctgttttc cgctcatgat ttatggttcc ccgtcatgac tcccgtaacg gcgatactgt
      901 gcgtagcctt ggcgtgcagt ctgttccccc agctctcccc cccctcccgc gatgcagagg
      961 acgaagatga agaagacgac ggcacaccct cgtcggaagg cagcggagcc ggccatgtgc
     1021 tgcccccgga caacttgtat caggaaccca acgaagtacc tatcccccat cactccaaaa
     1081 gagaacgccc cgactcatga aacagcgcaa gcacatcctc ttcatctgca ccggcaacac
     1141 ctgccgcagc cccatggcgg aaggcctgtt ccggaaactt tccgcgcacc atccggaatg
     1201 gcaggccggt tccgcaggaa cagccgcatg gcccgggcag gcagccagtc cggaaacact
     1261 ccacgtcctc cgggagcatg gcgtcaatct ctccagccat gaaagccgcc ctgttacgga
     1321 tgaactgatg aaaaacgcca cagatgtcta cgccatgacg gaaagccacc ttgccgccct
     1381 gcttgccaat ttccccgaac atgcggacaa ggtcaagctg gtcacctgct acacgggcaa
     1441 ccgtggcatc gccgacccca tcggctgcgg acagacagcc tacaacaatg tagcgcggca
     1501 attgacaacc gccatccagg ccatcattgc ccggatggag caacaggagt agcaacctct
     1561 ttgactcctt cccccgtcat ggcttcccct tccatacgca ttaatctgtc ctgccaggaa
     1621 ctggtgctgg aaaaatccgg aaaaatcctg ctccaatgcc ccgtttccag cgggaaggc
//
LOCUS       DAOMSU010000391         1679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_7958_length_1679_cov_2.659483, whole genome shotgun sequence.
ACCESSION   DAOMSU010000391 DAOMSU010000000
VERSION     DAOMSU010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7958_length_1679_cov_2.659483"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(228..425)
                     /locus_tag="PK077_11000"
     CDS             complement(228..425)
                     /locus_tag="PK077_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24864.1"
                     /translation="MMTAVSASQPGWLKGQRILRIGSGIHSVRLKKEYAVHGGIILLD
                     TGIRNVNGLPFVYFGNVNTTF"
     gene            complement(422..625)
                     /locus_tag="PK077_11005"
     CDS             complement(422..625)
                     /locus_tag="PK077_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24865.1"
                     /translation="MKSFLAAILSLFIPGLGQLYKGHFVQAIIWFLVVGAAYGLLYWF
                     IFALVPIVLHIICILQAYFMDNE"
     gene            874..>1679
                     /locus_tag="PK077_11010"
     CDS             874..>1679
                     /locus_tag="PK077_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRN24866.1"
                     /translation="MSIELTRNFSIIAHIDHGKTTLSDRLLEKTNTISEREKQDQLLD
                     AMDLEREKGITIKSHPVTIFYKARDGKTYKLNLLDTPGHVDFSYEVSRSLAACEGALL
                     IVDAAQGVEAQTLANMHLAMDLNLAIIPVINKIDLPSANLPKVYRQLEDIVCIPHEEA
                     IHASAKMGIGIDDILEAVVQRIPPPETEQDGFLRALVFDSVYDAYRGVVSYVRVISGS
                     VHRGMKVKLFATDEVYEVKEVGIFTPKMTRTDSLEAGDVGYIIANMKSAA"
BASE COUNT          429 a          483 c          428 g          339 t
ORIGIN      
        1 cgtcaacggc aacgcggaca tccgtgacgg gtccagctcg gtgaacggaa gcgtcggcta
       61 ccgctacgac ttctaacagg aagaaacggg aaaggaggct ccccacagcc tctttcctcc
      121 gggtcctcca gagccgttcc ggaacaactc cggaacggct ctgagttttt gtttatcgta
      181 ggattatccc cctggcccac ttcccgaaaa tattgattaa ttcaaagtca aaaagttgtg
      241 tttacgtttc cgaagtaaac aaatggaagc ccgttaacat tgcgaatacc ggtatcaaga
      301 agaataatgc cgccatgaac ggcgtattcc ttcttcaacc ttacggaatg aatgccgctg
      361 ccaatccgca ggatgcgctg ccctttcagc catcctggct gtgaagccga aacggcagtc
      421 atcattcatt gtccatgaag taggcctgca aaatacaaat gatatgcagg acaataggaa
      481 cgagtgcaaa gatgaaccaa tacaggaggc cgtaagccgc tcccaccacc aggaaccaga
      541 tgatggcctg gacaaagtgg cccttgtaca actggccgag accgggaata aacaggctta
      601 aaatggctgc taaaaaagat ttcatatgct ggctgagtaa gaaatgaccg cacgggaaaa
      661 gattccgcgt gcctgtacca gttgagagcc ttccttcttc ccccacgttc aatctcccgg
      721 cgcagcccct gtcatgaatg acggttttcc cggagcgaat gaagccgacg cggcagggaa
      781 cgggaggcag tcatgttcaa gtcttgaccg ccgcatgggc tatgttaccc tccccgcgca
      841 cagggcactc atgttagaag ggtagagacc accatgtcca ttgaactgac tcgcaatttc
      901 tccattatcg ctcacatcga ccacggaaag accacccttt ccgaccggct gctggaaaag
      961 acgaatacca tttccgaacg ggagaagcag gatcagcttc tggacgccat ggatctggag
     1021 cgggaaaagg gcatcaccat caagtcccac cccgtcacca ttttctacaa ggccagggac
     1081 ggaaaaactt acaagctcaa tctgctggat acgccgggcc atgtggattt ttcctacgaa
     1141 gtgtcccgtt ccctggcggc atgcgaggga gccctcctta ttgtggacgc ggcccaggga
     1201 gtggaagccc aaacgctcgc caacatgcac ctggccatgg atctgaacct ggccatcatc
     1261 cccgtcatca ataagattga cctacccagc gccaacctgc ccaaagtgta ccgccagctg
     1321 gaggacatcg tctgcatccc ccatgaggaa gccatccatg cctccgccaa gatgggcatc
     1381 ggcattgacg atattctgga agccgttgtt cagcgcatcc ctccgccaga aacggagcag
     1441 gacggctttt tgcgcgccct ggtgttcgat tccgtttacg acgcctatcg cggcgtggtc
     1501 tcctatgtac gcgtcatttc cggcagcgtg caccgcggca tgaaagtcaa acttttcgcg
     1561 acggacgaag tgtatgaagt gaaagaagtg ggcatcttca cgcccaaaat gacccgaacg
     1621 gattccctgg aagccggcga cgtaggctac atcatcgcca acatgaaatc cgcggcgga
//
LOCUS       DAOMSU010000392         1673 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8006_length_1673_cov_1.830037, whole genome shotgun sequence.
ACCESSION   DAOMSU010000392 DAOMSU010000000
VERSION     DAOMSU010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1673
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8006_length_1673_cov_1.830037"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(210..1337)
                     /gene="sucB"
                     /locus_tag="PK077_11015"
     CDS             complement(210..1337)
                     /gene="sucB"
                     /locus_tag="PK077_11015"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006554 - lysine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HRN24867.1"
                     /translation="MSDILTPNFGESVTSATVAAWHKNAGDPVAKGDTLVTLETDKVS
                     TDLEAEESGVLEILVPEGEEALIGAVLGRISSADGTAAAPERETPLIPLEPKKTTSPK
                     TREAPVSAPSAPAAEFPGQKPHGKVPLPEQVPAGKNREAEKEIVLRFIRKPMSPLRRT
                     IAARLVESQRQAAILTTFNECDMSSVMELRKQFHAEFRERHGTKLGFMSFFIKAVVKA
                     LQEVPQVNARIDGADIVENLYYDISVAIGTDKGLVVPVLRDCDRKTFPELEQELAVLT
                     EKARSGNLSMQDLQGGCFTISNGGTYGSLLSTPILNPPQSGILGMHAIQERPVVRDGQ
                     IVVRPMMYLALSYDHRLVDGKQAVQFLVAVKNAVENPVFEL"
     gene            complement(1353..>1673)
                     /gene="sucA"
                     /locus_tag="PK077_11020"
     CDS             complement(1353..>1673)
                     /gene="sucA"
                     /locus_tag="PK077_11020"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF028180.2"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HRN24868.1"
                     /translation="AAYRRERNISDTVIIRLEQIYPLAQEQLTYLLAPYQKVRDFVWC
                     QEEPSNMGAWGHLRNRLGHLFATSFRYAGRPPMACPAEGAKALHAAAQKRLIAAAFGP
                     RAQS"
BASE COUNT          318 a          447 c          498 g          410 t
ORIGIN      
        1 atatatgctt taactatcca gttcgtcaag aggagggcag tattttttat aaagagcttg
       61 aagaatcccc ctccctaaaa attttcccct tcccttgaaa agcgcctctt gacaaaagac
      121 ctgggggatt catttcctga atacttggaa aattaaaaaa gatatttgtt tttttcttct
      181 tgttatttag actttttccc tgcggctcct cataactcga atactggatt ttccacggcg
      241 tttttgacgg caacgagaaa ttgcacggcc tgcttgccgt ccaccaggcg gtggtcgtag
      301 gaaagggcca ggtacatcat gggccggacg acgatttggc cgtcccggac cacggggcgt
      361 tcctgaatgg cgtgcatgcc cagaatgccg ctttggggag ggttcagaat cggggtggaa
      421 agaagcgatc catacgtgcc gccgttggaa atggtaaagc atccgccttg cagatcctgc
      481 atggacaggt ttccgctacg tgctttttcc gtcaggacgg cgagttcctg ttccagttcc
      541 ggaaacgttt tccggtcaca gtcccgcagc acggggacga cgaggccctt gtccgtgcca
      601 atggctacag agatgtcata gtacaggttt tccacgatgt ccgcgccgtc aattctggcg
      661 ttgacctggg gcacttcctg caaggccttg actactgcct tgataaagaa gctcatgaaa
      721 ccaagcttgg ttccgtgccg ttcccggaat tctgcatgga attgtttgcg gagttccatc
      781 acggaggaca tatcgcattc gttgaacgtg gtgaggatgg ccgcctggcg ctgggactcc
      841 accagccggg ccgcgatggt gcggcgcagc ggactcatgg gttttctgat gaagcgcaga
      901 acaatttcct tttctgcctc cctgttcttt ccggcaggaa cctgttccgg aagggggact
      961 ttcccgtgtg gcttttgtcc cggaaattcc gcagctgggg cggaaggcgc ggaaacgggg
     1021 gcctcccgtg ttttcggcga ggtcgttttt ttcggctcaa gcggtatgag cggagtttcc
     1081 ctttccggtg cggctgcggt tccgtcagcg gaagaaatgc ggccaagaac tgcgccgatg
     1141 agggcttctt ccccttccgg aacgaggatt tccagtacgc cgctttcctc cgcctccaaa
     1201 tccgtggaaa ccttgtccgt ttccagcgtc accagcgtat cgcctttggc taccggatcg
     1261 ccggcgttct tgtgccacgc ggctacggtg gcggaggtga cggattctcc aaagttggga
     1321 gtcaggatgt cgctcatggg aggaagatgg gttcaggatt gcgcccgcgg tccgaaggca
     1381 gcggcgatga gtcttttttg tgcggcggcg tgcagcgctt tggccccctc cgcagggcag
     1441 gccatgggcg gccgtcccgc atagcggaaa gaggtggcga agaggtgccc cagcctgttc
     1501 cgcaggtggc cccaggcccc catgttggag ggttcctcct ggcaccagac gaagtcgcgc
     1561 actttctggt agggtgccag cagataggta agctgttcct gcgccagagg atagatttgt
     1621 tccagacgga tgattaccgt gtctgaaata ttccgttccc tgcggtaggc agc
//
LOCUS       DAOMSU010000393         1663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8106_length_1663_cov_2.254353, whole genome shotgun sequence.
ACCESSION   DAOMSU010000393 DAOMSU010000000
VERSION     DAOMSU010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8106_length_1663_cov_2.254353"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PK077_11025"
     CDS             <1..573
                     /locus_tag="PK077_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24869.1"
                     /translation="IQQHLGVDADGIPGPKTALALLEKLDIRQPEHSWPSQEDVRSGK
                     SVFGRAGDESNMTSIKLPYVMRLAWETNTTVSTMRCHKLVAEPLVRIFQATLDHYGME
                     KIRELGLDLYGGCFNNRSIIGGKATSMHAWGIAVDMDPDRNGLNVSAPKAVLSGPEYT
                     AFWQFVEAEGAVSLGRSRDYDWMHFQFATL"
     gene            complement(563..1585)
                     /locus_tag="PK077_11030"
     CDS             complement(563..1585)
                     /locus_tag="PK077_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HRN24870.1"
                     /translation="MHLTFLRHAGLAAARILMMAAWFGLSVWAAGVVFYNVWGGPVLV
                     WLYVAAMACAFALRRKRPVLWRASWGVPALLLAYYLCIPATNDKEWQPSWSRLPSVEI
                     NGNEIVVKDVRSFIYRTERDFDARYVTRRFDLDKLATLDFAVSHWDGMEFVAHTMLSF
                     GFEDGKHLALSVETRLPEGVEQGSVPGLYKQFNVIYILADEEDLFALRTNYRKEDMYL
                     YRINIDRENLKKAFLGFAEKINSLHERPRYYHTVTANCTTELVDTFKNYLGVRRWQWT
                     PVFNGMCDQNAYDRGELLHLPGESFRELKKRSFMGHGGNGEDWPALRRRWEEGWRTFA
                     SAPVKE"
BASE COUNT          371 a          516 c          461 g          315 t
ORIGIN      
        1 attcaacagc acctgggcgt ggatgctgac ggcatccccg gccccaagac ggctctggcc
       61 ctgctggaaa agctggacat ccgccaaccg gaacattcct ggcccagcca ggaggacgtg
      121 cgttccggaa aatccgtctt cggccgtgcg ggagatgaaa gcaacatgac cagcatcaag
      181 ctgccttatg tcatgcggct ggcgtgggaa acaaacacga ctgtttccac catgcgctgc
      241 cacaagctgg tggcggaacc gctcgtccgc atcttccagg ctactctgga ccattacggc
      301 atggagaaaa tcagggaact ggggctggac ctgtatggcg gctgcttcaa caaccgctcc
      361 attatcggcg gaaaagccac atccatgcat gcctggggca tcgccgtgga catggatccg
      421 gacagaaacg gactaaatgt ctccgcgccg aaagcggttc tgtccggacc ggagtacacc
      481 gctttctggc aattcgtgga agcggaaggg gccgtatccc tgggacgctc ccgcgactac
      541 gactggatgc acttccagtt cgctactctt tgaccggagc ggacgcgaac gtcctccagc
      601 cctcctccca gcggcggcgg agggctggcc agtcctcacc gttccctcca tgccccataa
      661 aagagcgctt tttcagctcc cggaagcttt ctccgggaag gtggaggagt tccccgcggt
      721 cataggcatt ctgatcgcac atgccgttaa aaacgggagt ccactgccag cggcgcacgc
      781 ccagatagtt cttgaacgta tccaccagtt ccgtcgtgca gttggcggtg acggtatgat
      841 aatagcgcgg gcgttcatgg agactgttga ttttttccgc aaaccccaga aaggcctttt
      901 tcaggttttc cctgtcaatg ttgatacggt acaggtacat gtcctctttc cggtaattag
      961 tgcgcagggc gaacaggtct tcttcgtccg ccaggatgta aatcacgttg aattgcttgt
     1021 aaaggccagg gacacttccc tgctccaccc cttcaggcag ccgcgtttcc acggacaggg
     1081 ccagatgctt cccatcctca aaaccgaagg acaacatggt atgggccacg aattccatcc
     1141 catcccagtg gctcacggca aaatccagcg tagccagctt gtccaggtca aagcggcgcg
     1201 tcacatagcg cgcgtcgaag tcccgctccg tccggtagat aaaactgcgc acgtccttca
     1261 ccacgatttc atttccgttg atttccacgg agggcaggcg actccaggac ggctgccatt
     1321 ccttatcgtt cgtcgccggt atgcacaggt agtacgccag caacagggcc ggcactcccc
     1381 aggaagcgcg ccataatacg ggccgttttc tgcggagggc gaaagcgcac gccatggccg
     1441 ccacgtacaa ccacaccagc acgggacctc cccatacgtt gtaaaaaacc acgcccgccg
     1501 cccatacgga gaggccgaac cacgccgcca tcatcagaat gcgggcggcg gccaggcccg
     1561 cgtggcgcag gaaagtcaaa tgcatgaaaa ataaaaagca ggccgcgcgt tccagctcat
     1621 gaaacgcgcg gcccgttaac ggaggttacc tgaaggaaag gcc
//
LOCUS       DAOMSU010000394         1660 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8132_length_1660_cov_2.333333, whole genome shotgun sequence.
ACCESSION   DAOMSU010000394 DAOMSU010000000
VERSION     DAOMSU010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1660
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8132_length_1660_cov_2.333333"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..789)
                     /locus_tag="PK077_11035"
     CDS             complement(<1..789)
                     /locus_tag="PK077_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRN24871.1"
                     /translation="MLHLYDTRTRTAQDISPMDGKTLRFYCCGPTVYGPAHIGNFRTF
                     VMQDVFRRVLELGGVPTTHIRNLTDVDDKTIRDSQKAGVSLAEFTAGWADLFHRDCAA
                     LNCLPPHAEPSAVGHIPEQIRMVQTLVEKGHAYVSEDGSVYFRISSFPEYGRLSHLDE
                     RELDLGKTANTRSNADEYEKDSVADFVLWKSRRPEDGNNFWPSPWGEGRPGWHLECSA
                     MIHKYFGNDFDLHSGGVDLVFPHHENEVAQSRCACGGGFARLWFH"
     gene            complement(911..>1660)
                     /locus_tag="PK077_11040"
     CDS             complement(911..>1660)
                     /locus_tag="PK077_11040"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HRN24872.1"
                     /translation="FPYIHIGGDECPMGDWKKCPDCQAARAKKGQGDNVEAQMSDFTK
                     SLTAMLAKHRKKPILWYDINKSYYHKGETVMSWLPGEFPRCIDKTKEQGIDLIVTPQF
                     KYYLARTQMKFPADDVRARPGGAPILLKDCYNFDPRNGRDKNDVKHIKGINLCMWAEW
                     IPSGELLMYMTYPRAMAVSETAWGSHKNRPSLEEFEKKMETHKKHFQKRFGYTLERTV
                     ENKPYREKFITQEEIERINENYKKGQQNADK"
BASE COUNT          321 a          435 c          498 g          406 t
ORIGIN      
        1 gtggaaccac aggcgcgcga agccgccgcc gcaggcgcag cgggactggg ccacttcgtt
       61 ttcatggtgg gggaatacca gatccacgcc gccggagtgg agatcaaagt cattgccgaa
      121 gtatttatgg atcatggcgg agcattccag gtgccagccg gggcggcctt ctccccaggg
      181 agagggccag aagttgtttc cgtcttccgg cctgcggctc ttccacagca cgaagtctgc
      241 cacggagtct ttttcatatt cgtctgcgtt ggaccgggta ttggcggttt ttcctaaatc
      301 cagttcacgt tcgtccaggt gggaaagcct tccgtattcc gggaaggaag aaattctgaa
      361 atacacggaa ccgtcttccg atacataggc atggcccttt tccaccagtg tttgaaccat
      421 ccgtatttgt tcgggaatat ggcccacggc ggagggttcc gcatggggag gcaggcaatt
      481 aagggcggca cagtcccggt ggaacagatc cgcccagcct gcggtgaatt ccgccagaga
      541 aacgccagcc ttttgagaat cccggatagt tttgtcatcc acatccgtca gattgcggat
      601 atgcgtggtg ggcacccccc ccagttccag gacgcggcgg aagacgtcct gcatcacgaa
      661 agtgcggaaa ttgccgatgt gggccgggcc gtacaccgtg ggtccgcaac agtaaaagcg
      721 cagtgttttt ccatccatgg gagaaatgtc ctgggccgtt ctggtgcggg tatcgtaaag
      781 gtgtaacatg gcggaacgat tagaagggaa gagggcttcc cggtcaagcg ggaaaagaaa
      841 gccggggatc cgcggttgtc cgcgtccccg gctttgaaga aatcagagga tggcgagaga
      901 aagggaaacg ctatttgtcc gcgttttgct ggcccttttt ataattctcg ttaatacgtt
      961 cgatttcctc ctgggtgatg aatttttccc ggtagggttt gttttccaca gtgcgttcca
     1021 gagtatagcc gaaacgcttc tggaaatgtt tcttgtgggt ttccattttc ttttcaaact
     1081 cttccaggct tggacggttc ttgtggctgc cccacgcggt ttcggaaaca gccatggcgc
     1141 gggggtaggt catgtacatc agcaattcgc cggagggaat ccattccgcc cacatgcaga
     1201 ggttgattcc cttgatgtgc ttgacgtcat tcttgtcccg tccgttgcgg ggatcgaagt
     1261 tgtagcagtc tttcagcagg atgggagctc cgccgggccg ggcgcgcacg tcgtccgccg
     1321 ggaatttcat ctgggtacgc gccagataat acttgaactg gggggtgacg atgaggtcga
     1381 tgccctgttc cttcgtctta tcaatgcaac gcgggaattc tcccggcagc caggacatga
     1441 cggtttcccc cttgtggtaa tagctcttgt tgatgtcata ccacaggatg ggctttttcc
     1501 ggtgcttggc gagcatggcc gtcaggcttt tcgtgaaatc gctcatctgg gcttccacat
     1561 tatccccctg gccctttttg gctcgggcgg cctggcagtc cgggcacttc ttccagtccc
     1621 ccatggggca ttcgtcaccg ccgatgtgga tgtacgggaa
//
LOCUS       DAOMSU010000395         1659 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8139_length_1659_cov_2.900873, whole genome shotgun sequence.
ACCESSION   DAOMSU010000395 DAOMSU010000000
VERSION     DAOMSU010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8139_length_1659_cov_2.900873"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..897)
                     /locus_tag="PK077_11045"
     CDS             complement(169..897)
                     /locus_tag="PK077_11045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24873.1"
                     /translation="MKRSFIILSLLAAAGMAQAATPIYSWEWTAEGAQITAPNWNGSF
                     NYTSGNDYATFGSSSGTPYNSNITGIQGSFTVSFDLKNLSSTSNGWKDIFSLYTNGTV
                     SGESNSMVLEFTNNGELYLYNKGFGGQTGGNINTGLTWTDLQQDSWTNLSIVSDLSSQ
                     TLSVYVNSALAGTITGWNPSSPALTGSQFGASFGNTRPMNGTMDINNVKFYDGVVLPV
                     PEAATASLGLLGLAALMMRRRRCC"
     gene            1346..>1659
                     /locus_tag="PK077_11050"
     CDS             1346..>1659
                     /locus_tag="PK077_11050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419864.1"
                     /note="binds the polymerase to DNA and acts as a sliding
                     clamp; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRN24874.1"
                     /translation="MKFTISKQVFLDGLRQVASVVSSKTTLPILSNVKIEAENGQVRF
                     TATDLDVCITGVVPANVLREGTVTLPAKKLVSIISELPEAEVQVDVNQRNQATVECGR
                     SQ"
BASE COUNT          452 a          373 c          440 g          394 t
ORIGIN      
        1 ataccgtcgt tttgccgggg cgggttcttc ctcccggtgc ttggaccagg ccgggggaag
       61 tcttcacggg ggcatgaatc ggggaaagga attttcttaa tactgaaaag gccgcatgcg
      121 gaaaaacgtc ttcccgcatg cggcctttgg ctaatggtta aagttgcttc agcagcaccg
      181 gcggcggcgc atcatcaggg cggccagccc caacaggccc agggaagctg tagctgcttc
      241 cggcacggga agcacgacac cgtcataaaa tttgacgttg ttgatatcca tagtgccgtt
      301 catggggcgg gtatttccaa aagaagctcc aaactgggag cctgtcaggg cgggggagga
      361 tggattccac ccggtaatcg tacctgccag agcgctgttg acatacacgg aaagcgtttg
      421 ggaggaaaga tcggaaacga tgctcagatt agtccagctg tcctgctgga gatccgtcca
      481 ggtcaatccc gtattgatat tcccgccagt ttggccgccg aaccccttat tgtagaggta
      541 gagttctccg ttattagtga attccagcac catgctgttg gattctccgg agaccgttcc
      601 attggtgtac aggctgaaaa tgtctttcca cccattgctg gtggaactca ggtttttcag
      661 gtcaaagctc acggtgaaac tgccttggat acccgttatg tttgagttat agggagtgcc
      721 ggaactgctg ccgaacgtgg cgtaatcatt gccggaagtg taattgaagg aaccgttcca
      781 gttgggggcg gttatttgtg ctccttccgc agtccattcc caggaataga taggggtagc
      841 cgcctgggcc atgcctgccg ctgccagtaa agataaaata ataaatgagc gtttcacgga
      901 aattctgaaa tatgggttaa aatcaatccg ataggagggt agcggattat aaatccgggg
      961 tcaaattatt tttctgaaaa caaatcattt aacggaaaaa attacggaga agataaaaga
     1021 agagaaaaca gaaacggcat tcctgttgga aagaaaacgt acatttccaa taaaattacc
     1081 gcatcctggg aaaaaagaga caatatccgg atttataatc cgcacaagga tattgaagaa
     1141 atctgattcc ccggaatgga tttttaagaa tgcttaaata atttgaaaaa ccttttcatg
     1201 caagatatgc ccggagaaat tgcatattac tatatatagt atgcttttaa aaaaatactg
     1261 ccaaatatta gggtttgacg gagaaatggc ttgaatcaca ggaggcactt gtgagaggat
     1321 gcgaggcaga taaaagcaca gacacatgaa gtttaccatt tccaagcaag tatttctcga
     1381 cggtctgaga caggttgcca gcgtggtgtc ttccaagacg acgctgccca ttctttccaa
     1441 cgtcaagatt gaagctgaaa acggccaggt acgttttacg gctacggatc ttgacgtatg
     1501 catcaccggc gtagttcccg cgaacgtgct gcgcgagggc accgtgactc ttcccgccaa
     1561 aaagctggtg agcatcatca gcgagcttcc tgaagccgaa gtccaggtgg acgtcaacca
     1621 gcgcaaccag gcgactgtgg aatgcggccg ctcccagtt
//
LOCUS       DAOMSU010000396         1649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8223_length_1649_cov_2.578419, whole genome shotgun sequence.
ACCESSION   DAOMSU010000396 DAOMSU010000000
VERSION     DAOMSU010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8223_length_1649_cov_2.578419"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..732)
                     /locus_tag="PK077_11055"
     CDS             complement(85..732)
                     /locus_tag="PK077_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRN24875.1"
                     /translation="MMISPEQYIDGLEWRYACKKFDPEARLEQPTWHALAESLRLSPS
                     SLGLQLWKFVVVTNRELKARLREVSWNQSQVEDCSHYVVLCSRRDATRRDVDRYLAQI
                     EFTRRPSAEKLASSADFYTSYVEALAPEKMHAWLDCQVYIAVGFLLSAAAALRVDSCT
                     IGGMDRAKYDEILGLDGTPYRSVVGVALGYRAKDDAYAREAKVRFPAGEVIDIRA"
     gene            complement(765..>1649)
                     /gene="hisC"
                     /locus_tag="PK077_11060"
     CDS             complement(765..>1649)
                     /gene="hisC"
                     /locus_tag="PK077_11060"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HRN24876.1"
                     /translation="GAAFRLRSAIAEFCGVEFGQTVVGTGSSEVIELICHALLNPRAE
                     VVAAKHAFSMYPIMSKLFGAAYVEVPNKEDWTHDLDGFLAAITENTRVVFITNPTNPV
                     GTVVGQQEIDDFMAKVPEHVLVVFDEAYREFSDNPPDTLKFVREGRNVVVLRTFSKAY
                     GLAGLRVGYGIAPEPVCSMLHKARAPFNLHVLAQEAALAALEDREHVRRTVENNKEGM
                     RFYEQAFREMGLEWIPSQGNFILVKVGRGKQVFQDMLARGVIVRAQDGYGLPEWIRIS
                     VGTPAENARCIEVLKEVL"
BASE COUNT          368 a          524 c          461 g          296 t
ORIGIN      
        1 cacgacggca aaacgccccc ccacggcaaa aaacgggaac gggccgtccc tattagggcg
       61 gcccgttccg gacaaaaaat aaaatcaggc gcggatatca atcacctctc cggcgggaaa
      121 gcgcaccttg gcctcgcggg cataagcgtc atccttggcg cggtagccca gagccacccc
      181 caccacagag cggtacggcg taccgtccag gcccaggatt tcatcatact tggcgcggtc
      241 catgccgccg atcgtgcagg aatccacccg cagggcggcg gcggcgctga gcaggaaccc
      301 cacggcaatg tacacctggc aatccagcca ggcatgcatc ttttccggag ccagagcctc
      361 cacgtagctg gtataaaaat cggcggagga agccagtttt tcagcggagg gtcggcgcgt
      421 gaattcaatc tgggccaggt aacgatccac atcccgcctg gtggcgtcgc gccgggagca
      481 cagcaccacg taatgggaac agtcctccac ctgggactgg ttccaggaaa cctcccgcag
      541 gcgagccttc agttccctgt tggtcacgac gacaaacttc cagagctgaa gccccaggga
      601 ggagggactg agacgcaggc tttccgccag cgcatgccag gtgggctgtt caagacgggc
      661 ttcaggatca aattttttgc aggcgtaacg ccattccaga ccgtcaatat actgttccgg
      721 ggagatcatc atgcagggtg agacacgaaa aacggaaaaa ctgtttaaag aacctctttc
      781 aatacctcaa tgcagcgggc gttctccgca ggagtgccca cgctgatgcg tatccattcc
      841 ggcaggccgt aaccgtcctg cgcgcggacg atgactcccc tggcaagcat atcctggaac
      901 acctgcttgc cccggcccac tttcaccagg ataaagttgc cctggctggg aatccattcc
      961 aggcccattt cccggaaagc ctgctcataa aaacgcatgc cttccttatt attctccaca
     1021 gtgcggcgca catgctcccg gtcctccagg gcggccaggg cggcctcctg ggccagaaca
     1081 tgcaggttga acggagcacg cgccttgtgc agcatgctgc aaaccggttc cggcgcaatg
     1141 ccgtagccca cgcgcagccc cgccaggccg taagccttgg agaaggtgcg cagaacaacc
     1201 acgttgcggc cttctcgcac aaatttgagg gtatccggag gattgtcgga aaactcccgg
     1261 tacgcctcgt caaagaccac cagcacatgt tccgggacct tcgccatgaa atcgtctatt
     1321 tcctgctgtc ccactacggt gcctacggga ttggtgggat tcgtaatgaa cacgacgcgc
     1381 gtattctccg taatagcggc caggaagccg tccaggtcat gcgtccagtc ctccttgttg
     1441 ggcacttcca cgtacgcggc gccaaacagc ttggacatga tagggtacat ggaaaaggcg
     1501 tgtttggcgg ccaccacctc cgcacgggga ttcagcaggg catggcagat cagttcaatc
     1561 acttcgctgc tgcccgtgcc cactaccgtc tggccgaact ccacgccgca gaactccgca
     1621 atagcggaac gcaggcggaa agccgctcc
//
LOCUS       DAOMSU010000397         1625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8417_length_1625_cov_2.602548, whole genome shotgun sequence.
ACCESSION   DAOMSU010000397 DAOMSU010000000
VERSION     DAOMSU010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8417_length_1625_cov_2.602548"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="PK077_11065"
     CDS             <1..531
                     /locus_tag="PK077_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24877.1"
                     /translation="NTPEYAEAVRKSADFRRKYADMGNNGIRTGWSTAWLINLYAALG
                     DGNAAEDRMYTMLRHYINSNLFDIHPPFQIDGNFGFSSGVAECLIQSRIMQDGFQVIL
                     LAPALADGWKKGSATGLRTRGGLKVDLSWQNGRVQATATATRPGKFRFMHRGRKKDLS
                     MNKGETARLDFPPLSH"
     gene            583..>1625
                     /locus_tag="PK077_11070"
     CDS             583..>1625
                     /locus_tag="PK077_11070"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRN24878.1"
                     /translation="MTFRLGLLMAVVCIALSSCSKLFSPRGIGKIPDTDQTETLLKAH
                     VFHLADSIGERNVYHPGSMERSARYIEQKLEGMGYAVTRQAVRIPPSGKFGSVKDWTA
                     YNLIAIKKGTSPRPKTLIVGAHYDTKVGMDNWHDHGPARPSRTGTPGANDNASGVAAL
                     LETARALAATSTLHDVCLVAYANEEPPFYQTPAMGSAVHAKSVSHHPGRKKIIGMIAL
                     ETLGCYSPRVNKKRQSAVVAGLAGLPDRCDYVAFLSTNTGHKLARSCAEEFSALSRFP
                     VRSAVFPYYTRGVSWSDDWGYMKEGIPSFAATDTAFLRCDDYHETSDTAEKLDYPQFA
                     EVVQGLSKLIISLA"
BASE COUNT          346 a          513 c          426 g          340 t
ORIGIN      
        1 aatacgccgg aatacgcgga ggctgtacgc aagtccgccg atttccggcg caagtacgcg
       61 gatatgggaa acaatggcat caggaccgga tggagcacgg cgtggctcat caatctttac
      121 gccgcccttg gcgacggaaa cgccgcagag gacaggatgt acaccatgct gaggcactac
      181 atcaactcca acctttttga catccatccc ccgttccaga tagacggaaa tttcggcttt
      241 tcctccggcg tggccgaatg cctgatccaa agccggatca tgcaggacgg gttccaggtt
      301 atcctgctgg ctccggctct ggcagacggc tggaagaagg gttccgccac gggattgcgt
      361 acgcggggcg ggcttaaggt ggacttgtcc tggcaaaacg gccgggtgca ggccacagct
      421 accgctacgc gtccgggcaa attccggttc atgcatcgag gccggaaaaa ggatttgtcc
      481 atgaataagg gagaaacggc gcgtcttgat tttcctccgc tttcccatta ggattcccct
      541 ctctgcgtta cctcttcaat ttccgccatg catttctttt ccatgacctt caggctcggt
      601 ctgctcatgg cggtggtctg catcgccctc agctcctgtt ccaagctttt ttctccccgc
      661 ggaatcggaa aaataccgga cacggaccaa acggaaacgc tcctgaaagc gcatgtattc
      721 cacctggcgg attccatcgg tgaacggaac gtctaccacc ccggctccat ggagcgttcc
      781 gcccgctaca ttgaacaaaa actggaggga atgggttatg ccgtcacgcg ccaggctgtc
      841 cgtatcccac cctccggaaa attcggctcc gtcaaggact ggacggcata taatctcatc
      901 gccatcaaga aagggacctc cccccggccc aaaacgctca tcgtcggcgc acattacgat
      961 accaaagtgg gcatggacaa ctggcacgac cacggtcctg cccggccttc ccgcaccgga
     1021 acgccgggag ccaatgacaa cgcttccggc gtagccgccc tgctggaaac ggcacgtgcc
     1081 cttgcagcaa cttccaccct tcacgatgtt tgcctggtgg cctacgccaa tgaagagcct
     1141 cccttttacc agacgcccgc catgggcagc gccgtgcatg ccaaatccgt ctctcatcat
     1201 ccgggcagga aaaaaatcat cggcatgatt gccctggaaa cgctgggttg ctattccccc
     1261 cgggtgaata aaaaacgcca atccgccgta gttgccggat tggccggcct gccggaccgc
     1321 tgcgactatg ttgccttcct ctccaccaac acgggccaca aactggcacg ctcctgcgcg
     1381 gaggaatttt ccgccttgag ccgttttccg gtgcgttccg ccgtgttccc gtattacacc
     1441 cgcggcgttt cctggtctga cgactgggga tacatgaagg agggcatccc ctcctttgcc
     1501 gccacggata cggccttcct ccgttgcgac gattatcatg aaaccagtga tacggctgaa
     1561 aagctggact atccccagtt tgcagaagta gtgcagggac tttccaaact catcatttca
     1621 ctcgc
//
LOCUS       DAOMSU010000398         1622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8444_length_1622_cov_2.155073, whole genome shotgun sequence.
ACCESSION   DAOMSU010000398 DAOMSU010000000
VERSION     DAOMSU010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8444_length_1622_cov_2.155073"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="PK077_11075"
     CDS             complement(<1..213)
                     /locus_tag="PK077_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRN24879.1"
                     /translation="MDRIRVYTGGVASCNGYLFKTRDNAYVAVDAPSGFADWIYSKKP
                     DIIITDLLITHQHFDHVEDACRMRQIF"
     gene            complement(305..1306)
                     /gene="pyrC"
                     /locus_tag="PK077_11080"
     CDS             complement(305..1306)
                     /gene="pyrC"
                     /locus_tag="PK077_11080"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018969194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HRN24880.1"
                     /translation="MILELHSPLDMHLHLRDGDMMKLVAPLSSASFAGAVIMPNLVPP
                     VADAGAVQAYRQRVLDACGDDVFQPYMTAFFRSYSEKELSRLKELVFGIKLYPAGATT
                     NSEGGVKAMKDAEATLSIMQEMDIPLLVHGESHGFVMDREAEFLDVYRDLATRFPRLT
                     ICMEHITTAAAVQLLDEFENLAATVTLQHLLITLDDVAGGMLRPHLFCKPIAKRPEDR
                     EALLQAALSGHPRLMFGSDSAPHPIHAKEACGCAAGVFTAPIALPRLAALFDEHGALD
                     RLQGFVSGHACALYGLNPPARTVRLQRREMLVPDAYEGHGQKVVPMDAGCTIPWRLI"
     gene            complement(1443..>1622)
                     /locus_tag="PK077_11085"
     CDS             complement(1443..>1622)
                     /locus_tag="PK077_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24881.1"
                     /translation="LHNGCRPVPKEPGGFFAFGLLLFLHSVCCFKKAVRENSVFLRHW
                     DRICFYDSFSGDFAL"
BASE COUNT          395 a          467 c          454 g          306 t
ORIGIN      
        1 gaaaatctgg cgcatgcggc aggcgtcctc cacatggtca aaatgctggt gggtgatgag
       61 aagatccgtg atgatgatat cgggcttttt ggaataaatc cagtccgcga agccggacgg
      121 agcgtccacc gccacgtaag cgttatccct cgtcttaaag aggtaaccat tacaggaagc
      181 aactccaccc gtataaacgc gaatccgatc catgggcagg gattgtagtg agatcgcccg
      241 gaatatccag tataaagacg gttcccttaa atagctttct gtcatcttcc cctggaaaag
      301 ccccttatat cagtctccag ggaatggtgc atccggcatc catcggcacc actttctgtc
      361 catgcccttc ataagcgtcc ggcaccagca tttcacgccg ctgcagacgg accgtcctgg
      421 caggcggatt caacccgtac aaagcgcagg catgaccgga aacaaagccc tgcaaccggt
      481 ccagggctcc gtgttcgtca aacagggccg ccagacgagg aagagcgatg ggggcggtaa
      541 atacgccggc agcgcatccg cacgcttcct tggcatggat gggatggggg gcggagtcac
      601 tgccgaacat gaggcgggga tgcccggaaa gggcagcctg caacagggct tcccggtctt
      661 ccggcctttt ggcgatcggc ttgcagaaca gatgcggcct cagcatgcca ccggccacat
      721 cgtccaaagt aatgagaaga tgctggaggg ttaccgtggc ggccaggttt tcaaattcgt
      781 ccagcagctg cacggcggcg gccgtggtaa tatgttccat gcagatagtc agccggggga
      841 agcgcgtggc caaatcacgg taaacatcca ggaattcggc ctcccggtcc atcacgaagc
      901 cgtggctttc gccatgcacc agcaaaggaa tatccatttc ctgcatgatg gacagggtag
      961 cttccgcatc cttcatggcc ttcactccgc cctcgctgtt cgtggtggcc cctgccgggt
     1021 acagcttgat gccgaacacc agttccttga gccgggacaa ttccttctcg gaataggagc
     1081 ggaaaaatgc cgtcatgtac ggctggaata catcgtctcc gcaagcgtcc agcacccgct
     1141 gccggtaagc ctgcaccgca cccgcatcag ccaccggagg caccaggttg ggcatgatga
     1201 ccgccccggc aaaagaggcg gaacttaacg gagcgaccag cttcatcata tcgccgtccc
     1261 tcaggtgaag atgcatgtcc aacggagagt gcagttccag aatcatgccg acatcatgag
     1321 ggaagattgc gcccaaatca agcacgctcc cctgccctgc aggaaagcaa ttctccgacg
     1381 gtaagggaaa acgggcattt tcccgcaaac cggaagggat gcggtacgct ttgctgctgt
     1441 tgctacaaag caaaatcgcc ggaaaacgaa tcgtaaaagc aaattctgtc ccaatgcctc
     1501 agaaaaacgc tgttctcccg gacggctttc ttgaaacagc agacggaatg caggaacagt
     1561 aaaagtccga atgcaaaaaa gccgcccggc tctttgggaa ccgggcggca gccgttatgc
     1621 ag
//
LOCUS       DAOMSU010000399         1613 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8513_length_1613_cov_1.993582, whole genome shotgun sequence.
ACCESSION   DAOMSU010000399 DAOMSU010000000
VERSION     DAOMSU010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8513_length_1613_cov_1.993582"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PK077_11090"
     CDS             complement(<1..699)
                     /locus_tag="PK077_11090"
                     /inference="COORDINATES: protein motif:HMM:NF024870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRN24882.1"
                     /translation="MSLLQNIKRGVQQRPQRVIIYGPEGVGKSTLAAGLPAPLFLDTE
                     EGTQHMNVDRIQVDHYGAMLEALQDIYKEARNGNLPYKTLVIDTGDRLWDMCARQVIR
                     DYNASPKDGKISSIESIGYGKGYAQASEMFVNLLSVFDNCRSAGLHIAVICHCRVETV
                     NPPEGEAYTMYTIKINAPAKQAITAKEKLKEWGDAILFCNYVTTFTDGGKAKGGELRA
                     VYTEHRATWEAKNRH"
     gene            complement(1142..1333)
                     /locus_tag="PK077_11095"
     CDS             complement(1142..1333)
                     /locus_tag="PK077_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24883.1"
                     /translation="MKKMTNEQYWMRRDRAEKMGSLYGCPIDFSEDELKPRPGIVQNL
                     VFSALLVGIFAIIYFIVKS"
     gene            complement(1405..>1613)
                     /locus_tag="PK077_11100"
     CDS             complement(1405..>1613)
                     /locus_tag="PK077_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24884.1"
                     /translation="EIMKTEIDLDKLPDGCKSHLLAEAEEGLKPSEAIIRILERESFR
                     RGFRVHLTTPRDLPRPKNPKKPAA"
BASE COUNT          297 a          404 c          450 g          462 t
ORIGIN      
        1 gtgtcggttt ttggcttccc aggtggcccg gtgctccgtg tagacggcac gcagttctcc
       61 gcctttggcc ttgcctccgt ccgtgaaggt ggtcacgtag ttgcagaaca ggatggcgtc
      121 cccccattcc ttgagttttt ccttggcggt gatggcctgt ttggccggag cgttgatttt
      181 gatggtgtac atggtgtagg cttccccttc cggagggttc accgtttcca ctcggcagtg
      241 gcagatgacg gcgatgtgca gccccgcgct ccggcagttg tcaaagacgg aaagcaggtt
      301 gacgaacatt tcgctggcct gggcgtaccc ttttccatag ccgatgcttt caatggagga
      361 gatttttccg tctttggggg aggcgttgta gtccctgatg acctggcggg cgcacatgtc
      421 ccacaggcgg tctcccgtgt cgatgacgag cgttttgtaa gggaggtttc cgttccgggc
      481 ttccttgtag atgtcctgca gggcttccag catggcgccg tagtggtcta cctggatgcg
      541 gtccacattc atgtgctggg ttccttcttc cgtgtccagg aagagggggg cgggcaaccc
      601 ggccgccagc gtggattttc ccacgccttc cggcccgtag atgatgaccc gctgcggacg
      661 ctgctgcact ccgcgcttga tgttttgtaa taggctcata ttatttcctt gtttgattgt
      721 attcaggtcg ggtgtcagtt cctgctggcc ccggcctttt tggttatggg tagttggaaa
      781 gggtactgac ggagttacgt ttccgcttag cgggaggttt gttcatgtcc gtcctggttt
      841 tggatgtctt ttgggtcaaa tacctgtaca cgcttatcgc ggaaattctg tattcgcgtt
      901 gattggtgcc aatgtcttct atttcatggt tctgcaacag gttacgaacc tttttcctac
      961 cccataaaca agcagggtgt ttccgcagat cctcaagggt aagccatatt ttgccgtcga
     1021 acatgcgagt ggcattctct tcctcggact cattcaaaat caacaagcca cgttcatgaa
     1081 gggattctat ggtttgctcc acaatagagg ttacaaattg atctaatccg ttcataattc
     1141 actaagattt aacgatgaaa taaatgattg caaaaatacc aaccagcaga gcggaaaaga
     1201 caaggttctg tacgataccg ggccggggct tgagttcgtc ttccgaaaag tctatcgggc
     1261 agccgtaaag ggatcccatt ttctcggcac ggtcacggcg catccaatat tgttcgttcg
     1321 tcattttttt cattgttgtt cagggggcgg gttaaagctc gtgccagccg agcagcttca
     1381 attcgtcaat cagggcttct tccatcatgc tgcgggcttc ttggggttct tcgggcgggg
     1441 aagatcgcgg ggcgtggtca agtgaacacg gaatcccctg cggaatgatt ctcgttcaag
     1501 gatgcgaata atagcctccg aaggcttcaa cccttcttcc gcttcggcca gcagatggct
     1561 cttgcagccg tccggcaatt tgtctaagtc gatttctgtt ttcatgatct ctc
//
LOCUS       DAOMSU010000400         1603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8601_length_1603_cov_1.987080, whole genome shotgun sequence.
ACCESSION   DAOMSU010000400 DAOMSU010000000
VERSION     DAOMSU010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8601_length_1603_cov_1.987080"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>823
                     /locus_tag="PK077_11105"
     CDS             <1..>823
                     /locus_tag="PK077_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24885.1"
                     /translation="LTPLQIEQAVTEAWKHADGDSLIFKFLLAYGLPKSSVSRLESGA
                     LNAAARPGELLWKKKLFYKRAEPGLLMSAVDSCRRDPEVLKYAPRFIIVCDGGSWAAA
                     DMKTGEALEFPLKDLPRHYDFFLPWAGMEKTVITLENPADVRAAEKMGKIFDSLKKDN
                     PGLDSHAMNVFMARLLFCFFAEDSGIFEEERLFTDFIERATASDGSDLREQLERAFAV
                     MNLPPGSPERAAVPISAARFPYVNGGLFQGAHAVPRFSSRSRKALIDAGNLDWSD"
     assembly_gap    824..833
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <834..>1603
                     /locus_tag="PK077_11110"
     CDS             <834..>1603
                     /locus_tag="PK077_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24886.1"
                     /translation="NTDIFGNMFQACVAPDKRSCLGEHYTSVPNIMKVLRPLFLEELE
                     NAARQARGHEARARKFIERLSNIRFFDPACGSGNFLIVAFKEVRRLEMEVLESVPALL
                     PMPSVSISQFYGIEIDDFAHEIAMLSLWLAEHQMNQEFFERLGKRVPTLPLRPNEHIV
                     LGNALRLDWETVCPKTAPAQAAPLWTVEPLLQRAAPDVREPEIYIFGNPPYLGSKMQS
                     VAQKEEVKNIYGNSNSENISNLDYVCGWIGLGAEFIAGS"
BASE COUNT          328 a          439 c          473 g          353 t
ORIGIN      
        1 tgctgactcc tcttcagata gaacaggccg ttacggaagc atggaagcat gcggacgggg
       61 attccctgat ttttaaattc ctcctagcct atgggcttcc caaatccagc gtcagccgcc
      121 tggaaagcgg cgccttaaat gcggccgccc gtccggggga gctgctgtgg aaaaaaaagc
      181 ttttttacaa aagggcggag ccggggctgc tgatgagcgc ggttgactcc tgccgccggg
      241 atccggaggt attgaagtat gctccccggt tcatcatcgt ctgtgatggc ggttcctggg
      301 ccgcggcgga catgaaaacc ggggaagccc tggaattccc cctgaaggat ctgccccggc
      361 attatgactt ttttctcccc tgggcgggga tggagaagac ggtcatcacg ctggagaatc
      421 cagccgacgt tcgcgccgcg gagaagatgg ggaaaatatt tgactccctg aaaaaagata
      481 acccggggct ggacagccac gccatgaatg tgtttatggc gcgcctgctg ttctgctttt
      541 tcgcggaaga cagcggcatt tttgaggaag aacggctttt tacggatttc attgaacgcg
      601 ctacggcttc ggatggatcc gacctgcggg aacagctgga acgggcgttt gccgtcatga
      661 atcttccgcc gggtagcccg gaacgcgccg ccgtcccgat ttccgcggcg cgtttccctt
      721 acgtcaacgg cggcctgttc cagggggcgc acgccgtgcc gcgcttttcc tcccgttccc
      781 ggaaagcgct cattgacgcg gggaatctgg attggtcaga catnnnnnnn nnnaatacgg
      841 atattttcgg caacatgttc caggcctgcg tggcgccgga caaacgctcc tgcctggggg
      901 agcattacac ttccgttccc aacatcatga aggtgctgcg ccctttgttc ctggaagagc
      961 tggaaaacgc cgcccggcag gcccggggcc atgaagcccg ggccaggaag tttattgaga
     1021 ggctcagcaa catccgtttt tttgaccccg cctgcggttc cggcaacttc ctgattgtgg
     1081 cgttcaaaga agtgcgccgg ctggaaatgg aggtgctgga aagcgtgccc gctctgctgc
     1141 ccatgccctc cgtttccatc agccagtttt acggcattga aatagatgac tttgcccatg
     1201 agatcgccat gctttccctc tggctggcgg aacaccagat gaatcaggag ttttttgaac
     1261 gcctgggcaa acgggtgcct acccttccgc tccgtcccaa tgagcacatc gtgctgggca
     1321 acgccctgcg cctggactgg gaaaccgtgt gcccgaagac ggctcccgct caggctgcgc
     1381 ccctttggac ggtggagccc ctgcttcagc gcgccgcgcc agacgtcaga gagccggaga
     1441 tttatatctt cggcaacccg ccgtatctgg ggtcaaagat gcagagtgtt gctcaaaagg
     1501 aagaagtcaa aaatatctac ggaaattcaa attcagaaaa tattagtaat ttagattacg
     1561 tttgcgggtg gattgggttg ggagcagaat tcatagctgg gtc
//
LOCUS       DAOMSU010000401         1596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8664_length_1596_cov_3.501622, whole genome shotgun sequence.
ACCESSION   DAOMSU010000401 DAOMSU010000000
VERSION     DAOMSU010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8664_length_1596_cov_3.501622"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /gene="nadC"
                     /locus_tag="PK077_11115"
     CDS             complement(<1..527)
                     /gene="nadC"
                     /locus_tag="PK077_11115"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HRN24887.1"
                     /translation="MPAETVSDNVETLINLALAEDFGSGDVTSTYFVPEHLTARAILT
                     PRKKGVLSGVNVAAEVFRKVDPTLKVEVYLHDGEAVAPGAVVMLIEGSARSILGAERT
                     ALNFIQRLSGVATLTRQYVKAISHTSARILDTRKTTPGYRLLEKAAVLHGGGTNHRMG
                     LYDRAMVKDNHLMTDG"
     gene            complement(542..1435)
                     /locus_tag="PK077_11120"
     CDS             complement(542..1435)
                     /locus_tag="PK077_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRN24888.1"
                     /translation="MCEQSLNDYITEQCALIDAALDRLLPAENESPETIHKAMRYSIF
                     AGGKRMRPVLCLAAAEACGGLAVDALVPACAVEMMHTYSLIHDDLPAMDNDDLRRGKP
                     TSHKAFGEGVAILAGDALLTEAFAVIAQVDDTERYTVRDYVRELAATGGSTMLIGGQI
                     LDLEGEHKQLSEPEVRTVYEGKTAALLTTALRFGGMSANATESQLEALTDFGYNLGLA
                     FQVIDDILDLTASTEKLGKTAGKDVNSQKSTCPALIGLDGARSEAKCRTQAALDALMI
                     FPEERRARLVELANYLLNREY"
BASE COUNT          293 a          446 c          501 g          356 t
ORIGIN      
        1 ccatccgtca tcaggtggtt gtctttcacc atggcgcggt cataaaggcc catgcggtgg
       61 ttggtgcctc cgccatggag cacggccgct ttttccagca gacggtagcc gggagtggtt
      121 ttgcgggtat ccagaatgcg ggcgctggtg tgggaaatgg ctttcacata ttgtctggtg
      181 agcgtagcta ctccggaaag acgctggatg aagttgaggg cggtgcgttc cgccccaaga
      241 atggagcggg cggaaccttc aataagcatc accacggcgc cgggcgccac ggcttccccg
      301 tcatgcaggt aaacttccac cttcagggtg ggatccacct tgcggaaaac ttccgccgcg
      361 acattgacgc cggaaagaac gccttttttc cggggggtca aaatagctct ggctgtcaga
      421 tgttccggga cgaagtatgt ggaagtcacg tcgccggagc cgaagtcttc ggccagcgcc
      481 agattgatca gggtttccac gttgtcggat acggtttctg cgggcatgaa agaaaagcgg
      541 cttaatattc gcggttaaga agatagttgg ccagttccac caggcgggcg cggcgttctt
      601 ccgggaaaat catcagggca tccagagccg cctgagtgcg gcatttggct tcggaacggg
      661 cgccgtccag gccaatcagg gccggacagg tggatttctg ggaattgacg tctttgccgg
      721 cggtcttgcc gagtttttcc gtggaagccg tcagatccag aatgtcgtca atcacctgga
      781 aagccagccc caggttgtaa ccgaagtccg tcagagcttc cagctgggac tccgtggcgt
      841 ttgcggacat gccgccgaag cgaagggccg tggttagcag ggcggccgtc ttcccttcgt
      901 acacggtgcg gacttccggt tcggaaagct gcttgtgttc tccctccaaa tccagaatct
      961 ggcctccgat gagcatggtg ctgccgccgg tggctgccag ctccctcacg taatccctga
     1021 cggtatagcg ttccgtatcg tccacctggg cgattacggc aaaagcctcc gtcagcagcg
     1081 cgtctcccgc caggatggcg acgccttctc cgaaggcctt gtggctggtg ggtttcccac
     1141 ggcgcagatc gtcgttgtcc atggctggca ggtcgtcatg gatgagggaa tacgtatgca
     1201 tcatttccac ggcgcaggcg ggaaccaagg cgtccactgc cagtcctccg caggcctctg
     1261 ccgccgccag gcaaaggacg gggcgcattc ttttcccccc cgcaaatatg ctgtagcgca
     1321 tggctttgtg gatggtttcg gggctttcgt tctctgctgg cagcagtctg tccagcgctg
     1381 cgtcaatcag ggcgcattgt tctgtgatgt agtcgttcag tgattgttca cacatggcgt
     1441 tatggaatag gtgcggggtg tggaggaaac aggtcagcgg ttgaccagca attccgcgat
     1501 ttgtacggcg ttgagcgcgg ccccttttcg gacctgatcg cccacgaccc agagagtcag
     1561 ggcattgtca atcgccatgt ccatgcgcat gcgccc
//
LOCUS       DAOMSU010000402         1594 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8684_length_1594_cov_1.968811, whole genome shotgun sequence.
ACCESSION   DAOMSU010000402 DAOMSU010000000
VERSION     DAOMSU010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8684_length_1594_cov_1.968811"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /locus_tag="PK077_11125"
     CDS             <1..832
                     /locus_tag="PK077_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HRN24889.1"
                     /translation="TRYFSGKGHNTSLDGPCGTLTTIPHQYPVTVTFMDNYFGNGYPT
                     SIDEPCGTLCTKQQRFPVTAVFLANYYSGGGQLASTESPCPTLTTNPKPRVVRCQFLD
                     QQYSQSGPASLDCPFPTITTNLHHGVVTASYIRAMMRPRAKGLVHSVDEPMKTLLTRD
                     YFYLDTCRYSYMGCPVYNQPAPGDTAAMLELKSAMRERGIADIYMRPISVRESLRVMG
                     FPEDYRLCGTQTQQRKWIGNAVEVKMARNMAQALADSMPGAVEVHGRQMAFEFRKETA
                     "
     gene            829..1395
                     /locus_tag="PK077_11130"
     CDS             829..1395
                     /locus_tag="PK077_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24890.1"
                     /translation="MKDWTGNNRTLGATLGASSHTTENRQREDYYATDPDMVRDLLNA
                     GAPLRQRVWEPACGAGHIATDIVDRGCPDSCVQDFLWEFDDGEIGDVDIMTNPPYATA
                     LEFVERALACVKNGANVWMLLRLQFLEGKARRRLYDVAPPADVWVFSERRTCAKNGDF
                     SKTEGGAIAYAWFHWVKGYNNQTIVKWL"
BASE COUNT          370 a          466 c          429 g          329 t
ORIGIN      
        1 gaccagatac ttttccggca aggggcacaa cacttccctg gacggcccct gcggaactct
       61 gacaaccatt ccccaccagt accccgttac ggtaactttc atggacaact acttcggcaa
      121 cggatatcct acctccattg acgaaccgtg cggaactctt tgtaccaagc agcaacggtt
      181 ccccgtcacg gcagtcttcc tggcaaacta ctattccgga ggcggccagc ttgccagcac
      241 ggaatccccg tgcccgacgc tgacgaccaa ccccaagccc cgcgtggtcc gctgccagtt
      301 cctggaccag caatacagcc agagcggccc ggcatccctg gattgtccgt ttcctaccat
      361 cacgaccaac ctccatcacg gagttgttac ggcatcctac atccgcgcca tgatgcgccc
      421 cagggccaag ggacttgtcc attccgtgga cgagcccatg aagaccctgc tgacgaggga
      481 ttatttttac ctggacacct gtcggtattc ctacatggga tgcccggttt acaaccagcc
      541 cgcccccgga gacacggcag ccatgcttga actcaagtca gccatgcggg aacgggggat
      601 tgccgacatc tacatgcgcc caatcagcgt ccgggaaagt ctgcgggtca tgggattccc
      661 cgaggactac cgcctttgcg gcacgcagac ccaacagcga aaatggattg gaaatgccgt
      721 tgaggtgaaa atggcccgga acatggccca ggcattggcc gattccatgc ccggcgccgt
      781 ggaagttcac ggcaggcaaa tggcttttga attcagaaag gagacagcat gaaagactgg
      841 accggcaaca accgaaccct tggcgccacg ctgggcgcat ccagtcacac caccgagaat
      901 cggcaacggg aagattatta cgccaccgat ccggacatgg tgagagattt actcaatgcc
      961 ggcgcgcccc tccggcaacg tgtatgggaa ccagcctgcg gagctggtca tatcgcaacc
     1021 gacattgttg accgtggatg tcctgattcc tgcgtacaag atttcttgtg ggagttcgac
     1081 gatggcgaga taggagacgt ggatattatg accaatcctc cctacgccac agcccttgaa
     1141 tttgtcgagc gtgcgcttgc ctgcgtcaag aacggagcca atgtctggat gcttctccgg
     1201 cttcaatttt tggagggcaa ggcccggcgc cgtctatatg acgtggcgcc gcccgcggac
     1261 gtgtgggtat tcagcgagcg gcggacctgc gccaagaacg gggatttttc caaaaccgaa
     1321 ggcggcgcca ttgcctacgc ttggtttcac tgggtcaaag gatataacaa tcaaactatt
     1381 gtgaagtggt tatgagagcc gtccttagat atcttggagc gaaaaataga attgccccat
     1441 ggattatccg tcattttccg gaccatacct gctacgtaga accgttctgt ggaagtttgg
     1501 gtatcctgtt caacaaagta ccggcccagg tggaaatctg caatgataag gatagcgaga
     1561 ttgttaatct gttccgcgta ctccgaagtg cgga
//
LOCUS       DAOMSU010000403         1589 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8729_length_1589_cov_2.255541, whole genome shotgun sequence.
ACCESSION   DAOMSU010000403 DAOMSU010000000
VERSION     DAOMSU010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8729_length_1589_cov_2.255541"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..>841
                     /locus_tag="PK077_11135"
     CDS             138..>841
                     /locus_tag="PK077_11135"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRN24891.1"
                     /translation="MFSKLKIHEKPGVVEWAERCLVLPRETSPNAPGRFSTARMPYMR
                     EPLESIREEGLQHIYWCFGTQSGKTVSLLIAAAYFIDNDPAPMLWALPTEILARSFSR
                     ARLQPLISKNDVLARHKRRDPDAFTAAEMRLDSMELYMVGVSEPGNLSSRPIMRCVMD
                     EEAKYKHENKEEAHPVDLIEERAKGFHRYQILHASTPSSEDSYFWQNFITTDMRKFYV
                     PCPRCGEMMPLEFSRNT"
     assembly_gap    842..851
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <852..>1589
                     /locus_tag="PK077_11140"
     CDS             <852..>1589
                     /locus_tag="PK077_11140"
                     /inference="COORDINATES: protein motif:HMM:NF017673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HRN24892.1"
                     /translation="RREDLEGDALADWVQDHTFYVCPHCEGRVEDWEKIGMMEKGEWR
                     PTNPNASRARRGYHLNSLYSPFVTWGQMARKFIVAQNDLFRQVALHNFRNGWEALPFT
                     QYEIKVGDDSVRGLRGVCRRGELPRHYYYLVVSYDPGQNQTHWVVQAIGRGGETWVVD
                     WGTLLGISTTDATPGIGAHFESLEWGGVRPDFGLIDSGDWAQKVYDECYKYYGKLWPT
                     KGSGANFGSWNVSEVKSHPGLELYLYV"
BASE COUNT          324 a          339 c          566 g          350 t
ORIGIN      
        1 gcagcagagg gactttatcg gttcccggct ggaggcggcc gggcggccgc gtttttatga
       61 agcctgggac gaatgggcgc gggagtggaa caggaagatt gatgatctga acgcggaaat
      121 aaatggattg ttgaatcatg tttagcaagt tgaagattca tgagaagccg ggcgtggtgg
      181 agtgggcgga aagatgcctg gtcttgccgc gggagacttc accgaacgcg ccggggcggt
      241 tttccacggc gcgcatgccg tatatgaggg aaccgctgga aagcatcagg gaagaggggt
      301 tgcagcatat ttactggtgc ttcggcacgc agtccggcaa gacggtttcg ctgttgattg
      361 cggcagcgta ttttattgac aatgaccccg cgcccatgtt gtgggcgttg cctacggaaa
      421 ttcttgccag gtcgttttca cgggcgcggc tccagccgct tatatccaag aatgatgtgc
      481 tggcgcggca taagcggcgt gaccctgacg ccttcacggc ggcggaaatg cgcctggatt
      541 ccatggagct ttacatggtt ggggtgtcgg agccgggcaa tttgtccagc aggcccatta
      601 tgcgctgcgt gatggacgag gaagcgaagt ataagcatga gaataaggaa gaagcgcacc
      661 cggtggacct gattgaagag cgcgcgaagg gctttcaccg gtatcagatt ctgcatgcgt
      721 ccacgccttc ttccgaagat tcttattttt ggcagaattt tattaccacg gacatgagga
      781 agttttatgt gccgtgtccg cgctgcgggg aaatgatgcc cctggagttt agccggaata
      841 cnnnnnnnnn naaaggcggg aggatctgga aggggatgcg ctggcggatt gggtgcagga
      901 tcatacgttt tacgtgtgcc cgcattgcga gggccgggtg gaggattggg agaagattgg
      961 gatgatggaa aagggggagt ggcggccgac gaatccgaac gcctcccgcg cgcggcgggg
     1021 gtatcacctg aattcccttt attctccgtt tgtgacatgg gggcagatgg cgcggaagtt
     1081 catcgtggct caaaatgacc tgttccggca ggtggccctg cacaatttcc ggaacggctg
     1141 ggaggcgttg ccgtttacgc agtatgaaat caaggtgggg gatgacagtg tgcgggggct
     1201 gcgcggtgtg tgccggcgcg gagagttgcc gcggcattat tattatctgg tcgtgtccta
     1261 tgaccccggc cagaatcaaa ctcattgggt ggtgcaggcg atagggcgcg gcggggaaac
     1321 atgggtggtt gattggggaa ccctgctggg catcagcacg acggacgcga cgccgggcat
     1381 aggggcccat tttgaaagcc tggagtgggg cggggtgcgt ccggattttg ggctgattga
     1441 ttccggggat tgggcgcaga aggtttatga cgagtgctat aagtattacg gcaagctatg
     1501 gcctacgaag gggagcggcg caaatttcgg gagttggaat gtgagtgaag tgaagtcgca
     1561 tccggggctg gagctttatt tgtacgtgg
//
LOCUS       DAOMSU010000404         1585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8760_length_1585_cov_1.845752, whole genome shotgun sequence.
ACCESSION   DAOMSU010000404 DAOMSU010000000
VERSION     DAOMSU010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8760_length_1585_cov_1.845752"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    222..268
                     /estimated_length=47
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            497..685
                     /locus_tag="PK077_11145"
     CDS             497..685
                     /locus_tag="PK077_11145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24893.1"
                     /translation="MTEQAFLFFLTDALRRVLMKPFPPNGVWLLIVDSHLRLAEYGRP
                     RLDLFSIASAGFFVPLLP"
     gene            736..>1585
                     /locus_tag="PK077_11150"
     CDS             736..>1585
                     /locus_tag="PK077_11150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HRN24894.1"
                     /translation="MVSTTFRELGLSAPILRQLENLEYKTPTPIQAASIPLLLRNKDL
                     LGLAQTGTGKTAAFALPLIQHLSERPGKPPGRRVRALILSPTRELAAQIHENILAYAR
                     GLHLSTAVIFGGVGYAPQFKKLADGLDILVATPGRLIDHLERRTVSLDSVETLILDEA
                     DHMLDMGFAPALKKIVSRIPEKRHTQMFSATMPENIRKLAQTLLQKPETVRVSPPSST
                     ADKVEQFLCMVGSQAEKRPLTLKLLKQQELPGRTLIFTRTRHGADRLASFLSGKDYPA
                     SAIHGDK"
BASE COUNT          365 a          433 c          385 g          355 t
ORIGIN      
        1 ccgctgtcca aatccggggc ttgcttaaat ccgccctttc ttccgattgg aaatccgcaa
       61 agagagggct gccgcaacat acggccaata cggaaaaaat gaccattcca ttctttattt
      121 ttgttttcag gggcataact catttaacat ggtttatcca atgaataagt atacggataa
      181 ggaatccgct gattggaata tgttgtctct aacatggcgg gnnnnnnnnn nnnnnnnnnn
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa ttggaacagg aacaggacct cttggacgtt
      301 tcccctaaat tgttcaccaa aaaatgaatc aggccattca tacgcagaat ttagggttga
      361 cggcggattc cttacccgct tcatccaata atccgtttat actcagttcc tataaaaaat
      421 tcatgacgaa aactgtttcc aaagcggagg ggaaaaggag cttccaggaa ccagccgcgt
      481 accggaatat ggagccatga cagagcaggc gtttttattt ttcttgacag atgctcttcg
      541 tcgtgttctg atgaagccgt tcccgccgaa tggtgtttgg ttgttgattg ttgatagtca
      601 tttgcggttg gcggaatacg ggcgtccccg tcttgacctt ttcagtatcg cttcagcggg
      661 cttttttgtt ccgttattgc cgtgattccg tcagttcatt gcagccgggg cgcattcaca
      721 gacacaccca cagacatggt atcgactaca ttcagggagc ttggcttgtc ggctcccatt
      781 ctccgccaac tggagaacct ggagtacaag acccccactc ccatccaggc cgccagcatc
      841 ccgctgctgc tgcggaacaa ggacctgctg gggctggcgc aaaccggaac cggcaaaacg
      901 gccgcgttcg ccctgcccct tattcaacat ctttcagaac gtcccggaaa accgccgggc
      961 cgccgggtga gggcgcttat tctgagtccc acccgggaac tggccgcgca aattcatgaa
     1021 aacatcctcg cctacgccag aggactgcac ctgtccaccg ccgtcatttt cggaggcgtg
     1081 gggtatgctc cccagttcaa aaagctggcg gacggtctgg atattctcgt agctacgccc
     1141 ggcagactga ttgaccatct ggaacgccgg actgtcagtc tggacagcgt ggaaaccctg
     1201 attctggatg aggcggacca tatgctggat atgggattcg ctccggccct gaaaaaaatc
     1261 gtctccagaa tcccggaaaa acgccacacg caaatgtttt ccgccacaat gccggaaaat
     1321 atccggaaac tggcccagac cctgcttcag aaaccggaaa cggtgagagt ctcccctccc
     1381 tcctccacgg cggacaaggt ggaacagttc ctttgcatgg tcggctccca ggcggaaaaa
     1441 aggcccctta ccctgaaact gctgaaacag caggagttgc ctggccgcac gcttatcttt
     1501 acccggacca ggcacggagc cgaccgcctg gcctccttcc tgtccggaaa ggattatccg
     1561 gcttccgcca ttcacgggga taaaa
//
LOCUS       DAOMSU010000405         1580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8800_length_1580_cov_1.803279, whole genome shotgun sequence.
ACCESSION   DAOMSU010000405 DAOMSU010000000
VERSION     DAOMSU010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8800_length_1580_cov_1.803279"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>430
                     /locus_tag="PK077_11155"
     CDS             <1..>430
                     /locus_tag="PK077_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HRN24895.1"
                     /translation="DATFLAQSKVIRDITASQACVIVGRCANFILKGRPNLFSVFLHA
                     DRNTRMQRVIENYGVKPGGAARTMDIMDSRRRTHCLHYTGQELGNARLYNLCVNTSDY
                     GLERTVELILEAINTRTEQSPASETAPVREPALPEPEAEDI"
     assembly_gap    431..440
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            730..1179
                     /locus_tag="PK077_11160"
     CDS             730..1179
                     /locus_tag="PK077_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HRN24896.1"
                     /translation="MRLWSLHPSYLDAVGLVALWREGLLARKVLQGQTKGYIHHPQLF
                     RFRETGNPIYVLDFYLKGVHDESIRRGYHFDLSKISPCENRPPSLLLPEKQLEYEFFH
                     LLDKLKERSPWQYSLLRQTAVILPHPLFQIKPGDICSWEKKPVSSLT"
     gene            complement(1397..1472)
                     /locus_tag="PK077_11165"
     tRNA            complement(1397..1472)
                     /locus_tag="PK077_11165"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1437..1439),aa:Ala,seq:ggc)
BASE COUNT          396 a          473 c          348 g          353 t
ORIGIN      
        1 gacgccacct tcctggcaca gagcaaagtc atccgggaca ttacggccag tcaggcatgc
       61 gtcatcgtgg gccgctgcgc caacttcatc ctgaaaggga ggcccaacct gttcagtgtt
      121 ttccttcatg cggacaggaa cacgcgcatg cagcgcgtta ttgaaaacta cggggtaaaa
      181 cctggcggcg cagcccggac catggacatc atggactccc gccgccgcac ccactgcctg
      241 cactacaccg ggcaggaact gggcaatgca cgcctctaca acttgtgcgt caacacgtcc
      301 gactatggcc tggagcgcac agtggaactg attctggaag ccatcaacac cagaaccgag
      361 caatcccccg cctcggaaac ggcccccgtc cgggaacccg cgctcccgga accggaggcc
      421 gaggacatcc nnnnnnnnnn cagaaccgaa caatcttcct ctgtagaaac ggttcccgtc
      481 cactccgcat cttttcccga accggaggcc ggcagcattc ccggagaaat atccctggcc
      541 tgaccggggc aaatgcctca aacaggcaat acttatgtaa aaactgcctg accggtaaat
      601 aagaaacccg gcaggaaagc agatggctcc ttccctataa aagcagcccc atccctccaa
      661 acttccccgg cttttttccg gctgcgcaat tcaaagcaac aagatacggc ctttctgagt
      721 ccaaaaagca tgagactctg gtcactccac ccctcttacc tggatgctgt cggcctggtt
      781 gccctgtgga gagaaggcct cctggcacgc aaagttttgc aggggcagac aaaaggctat
      841 atccatcatc cccagctctt ccgcttccgg gagacgggaa accccattta tgtcctggat
      901 ttctacctga aaggcgtcca tgacgaatcc atccgcagag gctaccattt tgatttaagc
      961 aaaatttctc cctgcgaaaa ccggccccct tcccttctcc ttccggagaa acagctcgaa
     1021 tacgaattct ttcatcttct ggacaagctc aaggaaaggt ccccgtggca atattccctg
     1081 ttgcggcaaa cggccgttat ccttccccat ccgctttttc aaataaaacc aggagatatt
     1141 tgctcctggg agaaaaagcc tgtttcgtca ttaacatgac ttcattccct tttccggaaa
     1201 ctattgatct ttttaatagt gacggcgatc aaaccacaga catacgccat tcccatctca
     1261 tcacccggca attactgcag cgaacagaat tactgtaaac ctttccaacc ttttgctttt
     1321 tcaggctcat acagtgaaaa aacatgtccg gcaaaggaaa aggccttaaa gcgttaaccg
     1381 ctttaaggcc tttatttggt ggagcatagc gggttcgaac cgctgacctc ttcaatgcca
     1441 ttgaagcgct ctaccaactg agctaatacc cctttgactt tcgtcttggt gcgtcctgtt
     1501 gaggacgggg gaaatctatc atttccaaag cgggcatgca agctttttct acaaaaaaat
     1561 tgttccggga taatttttcc
//
LOCUS       DAOMSU010000406         1575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8837_length_1575_cov_2.282237, whole genome shotgun sequence.
ACCESSION   DAOMSU010000406 DAOMSU010000000
VERSION     DAOMSU010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8837_length_1575_cov_2.282237"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            160..1107
                     /locus_tag="PK077_11170"
     CDS             160..1107
                     /locus_tag="PK077_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24897.1"
                     /translation="MSLRSQLEELLPELLPSDPAQAIKGTELIRLVRLRLGEEYSDAS
                     LRYHFSFMASEPDSEIAKVERGQGYYRRMRERDGQRAPRGLLPLFLGENEPDALNCRA
                     KALALAVRQYDTTGRGVFVFSTSETGTSWVKPDLAVVEWPEGEWEGNALVFDKSALQR
                     RRMIGAPMMGIRSVCVARMPGGEDGRREFFRTLATSRWAQCGELVIVGELPDDSECAA
                     LRGLAAEFGVGVLCLEIADERLGELPGAEEIFKAGDEECAALLAELTPVQLAAGRLKT
                     LEADTGETLGGEFGALFDWLAACLERGSVEEYEFRVSCY"
     gene            1187..>1575
                     /locus_tag="PK077_11175"
     CDS             1187..>1575
                     /locus_tag="PK077_11175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24898.1"
                     /translation="MEQETLVYPLADNTVLQDKYTILSVLNAGGFGITYLALDNPGSR
                     YVVIKECMPDAYACRDMETGFVRPRNEQTAVNFSQSVSNSRQEASVLSQLSHPGIVQV
                     FDMFDANGTCYYVMENIQGQTLFDLMTT"
BASE COUNT          328 a          450 c          473 g          324 t
ORIGIN      
        1 tacggccatg cgccctggcg aaggtatata accggaggta atccatgcca tgcggatcat
       61 cttgccgtat tccggagcaa ataaaagagc tttttatttc atgtgctgaa ctccttccgc
      121 cttgaagaag cgcttctccc gtgttctcat ggagcgcgta tgagtttacg atcccagctg
      181 gaagaactgc tcccggaact tttgccttcc gatccggccc aggccattaa gggaacagaa
      241 ctcatccgcc tggtgcgtct gaggctgggg gaggaatatt cggacgcatc cctgcgctac
      301 catttttctt tcatggcttc cgagccggat tcggaaatcg ccaaggtgga acgcggccag
      361 ggctattaca ggcgcatgag ggaacgggac gggcagcgcg ctcccagagg gcttttgccc
      421 ctgttcctcg gggaaaacga accggacgcc ctgaactgcc gcgccaaggc gctggccctg
      481 gccgtgcgcc agtacgatac cacgggccga ggcgttttcg tgttcagtac cagtgaaacc
      541 ggaacttcct gggtaaagcc ggatctggcc gttgtggaat ggccggaggg ggaatgggag
      601 ggaaacgcgc ttgtttttga caaaagcgca cttcagcgcc gccgcatgat cggggcgccc
      661 atgatgggca tccgcagcgt ctgcgtagcc cgcatgcccg gaggagaaga cggcagaagg
      721 gaatttttca ggactctggc cacgtcccgc tgggcacagt gcggagaact ggtcattgtg
      781 ggggaactgc cggatgattc cgaatgcgcc gccctccgcg ggctggccgc cgagttcggc
      841 gtaggggtgc tttgcctgga aattgcggac gaacgcctgg gagaactccc cggcgcggag
      901 gaaattttca aagccggaga tgaagaatgc gccgccctgc tggcggaact cacgcccgta
      961 cagctggccg ccggaaggct gaagacgctg gaggccgata cgggggaaac cctgggcggg
     1021 gaattcggcg ccctgttcga ctggctggcc gcctgcctgg aacgcggcag cgtggaagaa
     1081 tatgaattcc gcgtttcctg ttattgaata cgacggagag attcttgaca gaacttgcgt
     1141 attaaattaa agattttccc ggctccaacc cgccctctcc tctgacatgg aacaagaaac
     1201 cctggtttat ccgcttgcgg acaatactgt tctgcaagac aaatatacga tattgagcgt
     1261 gctgaacgcc ggcggcttcg gcatcacgta cctggctctg gacaaccccg gttcccggta
     1321 tgtcgtcatc aaggaatgca tgccggacgc ctacgcctgc cgggatatgg aaaccggttt
     1381 cgtccgtccc cggaacgaac agaccgccgt caatttctcc cagagcgttt ccaattcccg
     1441 gcaggaggct tccgtcctgt cccagctcag ccatcccggc attgtccagg tgtttgacat
     1501 gtttgacgcc aacggcacct gctattacgt catggagaat atccagggac agaccctgtt
     1561 cgacctgatg accac
//
LOCUS       DAOMSU010000407         1572 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8864_length_1572_cov_2.580092, whole genome shotgun sequence.
ACCESSION   DAOMSU010000407 DAOMSU010000000
VERSION     DAOMSU010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8864_length_1572_cov_2.580092"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..984
                     /locus_tag="PK077_11180"
     CDS             424..984
                     /locus_tag="PK077_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24899.1"
                     /translation="MNTSPTIGVLLAAGAILLPSCETERVNERPIGIYPTENVYPSGA
                     MGAQGDSLLNDIQRENSLDPMAGQNSGFNPDPARTNSGTISNMVGGANNITPPAPVVP
                     KPVVPNPVGGSAGSGIAAPRPVEPSASSSVPVAWPTADPTVVVSPYDRTKKIKILNRS
                     TNKPYPSGTVLRDTNFPNEIKKFRVP"
     gene            1065..>1572
                     /locus_tag="PK077_11185"
     CDS             1065..>1572
                     /locus_tag="PK077_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRN24900.1"
                     /translation="MREYSRKQFFFSSVAILGPGLLGGSLALAVRKQLPHVHVRLWGR
                     REEPLEYARSSGAAHAASTRMEEVVEGADLVVLATPVGVMARLVSDMLPLLKPGVLVT
                     DVGSGKGCVHQAVGSVLKKAGVAFIGSHPMAGSEKQGMEHASGDLFRDATCILTNDEH
                     VHEDVLLLL"
BASE COUNT          285 a          430 c          499 g          358 t
ORIGIN      
        1 gggaagcgtt tttcccagtt ttttgaagag ggcgggcagg ggagggccgg aggtcggcgc
       61 ggcatgttcg ggagggaagc gtctcttccc gctggagaac ggaatggagc tccggcttct
      121 tccgaataag gctgcggcag gagtacctgt tggagcgctt gttgataaag agtgatggtt
      181 gttccgtctt tgtgccgttt tccggctctt ccttttgttg agtccgtcca gggctgttcc
      241 ggcgctgccg atgggtaaaa gaaatgcggc gagcgcgtct gttcgggcgg ctttgcccgc
      301 gtgcggggac ggaagccgaa gattccttgt catgaagagg ttcggcaaac aggctttatg
      361 gttgacatga ggttgcgggg ccgtaggatg ctggcagaaa gctatcaccc gaagagagat
      421 tatatgaata cttccccgac gataggcgtg ttgctggccg caggtgcgat tcttctgcct
      481 tcctgtgaaa cggagcgcgt gaatgaacgc cccatcggca tttatcctac ggaaaatgtc
      541 tatccgtccg gcgctatggg cgcccagggc gatagcctgc tgaatgatat ccagcgtgaa
      601 aattctctgg acccgatggc cgggcagaat tccggtttta acccggaccc cgcccgcact
      661 aattccggga ccatcagcaa tatggtggga ggggccaaca atatcacgcc tccggctccc
      721 gttgtgccca agcccgtcgt tccgaatccc gtgggtggct ccgccggttc cggcattgcc
      781 gcccccaggc ctgtggaacc ttccgcttct tcttccgtcc cggtggcgtg gcctacggcg
      841 gatcccacgg tggtggtcag cccgtatgac cgcaccaaga agatcaagat tttgaacagg
      901 agcaccaata agccgtatcc ctccgggacg gtattgcggg acaccaactt ccccaacgag
      961 attaagaagt tccgcgtgcc ctgatgcggc cgccgcagcg agatcatgcc gcgcccctga
     1021 acatgccgat gttccggggc gctgatgtat tcccctgtaa atccatgaga gaatattccc
     1081 gaaagcagtt tttcttctcc tccgtcgcca ttctcggccc cggccttctg ggcgggtcac
     1141 tggcgctggc cgtcaggaag cagttgccgc atgtccatgt gcgtttgtgg ggcagaaggg
     1201 aagaaccatt ggaatatgcc cgttcttccg gagccgccca tgctgcttcc acccggatgg
     1261 aagaggtggt ggagggggcg gatttggttg tgctggctac cccggttggc gtgatggccc
     1321 gccttgtttc cgacatgctt cctttattga agccgggagt actggtaacg gatgtgggtt
     1381 ccgggaaggg gtgtgttcac caggccgtgg gctccgtgct gaagaaagcc ggcgtggcgt
     1441 ttatcggctc ccatcccatg gccggttccg aaaagcaggg tatggaacat gcttccgggg
     1501 acctcttccg ggatgcgacc tgcatcctga cgaatgacga gcatgtgcat gaagatgtgc
     1561 tgctgttgct cc
//
LOCUS       DAOMSU010000408         1570 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8884_length_1570_cov_2.480528, whole genome shotgun sequence.
ACCESSION   DAOMSU010000408 DAOMSU010000000
VERSION     DAOMSU010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8884_length_1570_cov_2.480528"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1113)
                     /locus_tag="PK077_11190"
                     /pseudo
     CDS             complement(101..1113)
                     /locus_tag="PK077_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor protein KdpD"
     gene            complement(1408..>1570)
                     /locus_tag="PK077_11195"
     CDS             complement(1408..>1570)
                     /locus_tag="PK077_11195"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF014699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit C"
                     /protein_id="HRN24901.1"
                     /translation="KRVAKARGMEEGAVRSLVDQAVEKPLLGMFGTEKVNVLKLNIAL
                     EKADPSGKH"
BASE COUNT          369 a          444 c          332 g          425 t
ORIGIN      
        1 caatccgttc cgctcaggga agacaagcat gataatctca tcgcctgctt cctgtgaaaa
       61 gagccggctc cggccttatt ttcaaattta ccgctgtttt ttatgcaatg atgattaaat
      121 ctatcttcat ctccgacaag acacgcagaa attgtttata tcccagtatc ttgaacatgg
      181 ctccgggcat tttgaatgcg ggatgcccca tgcaaatagc cgtaatatga tattccctgc
      241 acgttgcgat gatggcctgg ggtatgtctt ccgcactcac ctgcttcacc gtccctccca
      301 gttcggccac cagcttgaaa tgattaagca aatggcgctg ggacgccaga ttgatgcggt
      361 cgggggactc ccttggcgtc tggacgtaaa gggcgataaa cgacgtactg taacgcgagg
      421 aaatccggta tgcttttcgg atgatgcgcc ggggcgtctg ttcattgctg ctgatgcagg
      481 ccagaatctt ttcccttctg gcggaagaaa ctgccgggaa tacttccgtt tccacttttt
      541 tctccactcg gaaagcaact tccttcaatg ccagttcacg cagctgaaga atattttccg
      601 ttctgaaaaa attatccagg gcaatctgga ttttttcagg ccggtaaatc ttgccggcct
      661 tgaggcggcc gatcaattcc tcggacgtca ggtcgatatt caccacttcg tccgcctcct
      721 gcaaaatcct gtcgggaacc cgttccttca tctcaatccc cgtaatctcc ttcacttcgc
      781 cgctcaggct ttcgatgtgc tggatattaa tcgcggaaat cacattaata ttgcatcaag
      841 cagctccatc acgtcttccc atctcttgcg atggcggctc ccttccacat tggtatgagc
      901 cagttcgtca acaataacca actcgggccg ggcttgaaga atcgcgtcca ggtccatttc
      961 ttcaatctcc ctgcctttgt aaaaaaccac tttacgcggg atgacgggca ccccccggag
     1021 catggcttca gtcccgggcc gcccgtgggt ttcaacgtaa ccgattctga catcaatgcc
     1081 gttattcagc aattcatggg cttcctgcag catgcggtac gtcttgccta ctccggccat
     1141 cataccaatg taaatcttga actttcccct tcgagaacgc cgtatcaggt caagaaagtg
     1201 ctctgctccg ttacttccgt caatcatatt aaattcaccc ggctcctgcg gttaaaaaca
     1261 tcttgttgat tttacagaat cttttgaaaa tgaaaatcaa ttgcgggcgt taccatgaca
     1321 tgaagcccgc aattgatttc attttttcaa ggcggcaatt tatcaccaaa tccggaatcg
     1381 ccattgccgc cctaaacaat caggaaatta atgcttccct gaaggatcag ccttctcaag
     1441 agcaatattc aacttaagca cattgacctt ctccgtgcca aacatgccca gcagcggttt
     1501 ttccacggcc tgatcgacaa gggacctcac tgcaccctct tccataccac gtgctttggc
     1561 aacacgcttc
//
LOCUS       DAOMSU010000409         1562 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_8969_length_1562_cov_2.110153, whole genome shotgun sequence.
ACCESSION   DAOMSU010000409 DAOMSU010000000
VERSION     DAOMSU010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8969_length_1562_cov_2.110153"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="PK077_11200"
     CDS             complement(<1..254)
                     /locus_tag="PK077_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRN24902.1"
                     /translation="MRTFTWPIALLLAGLLFQTVAFAVLNTVVPLWMEQFDAATWEAG
                     LVGAFFFLGNLAGTLLAGGVIRRAGFKGSYQYACLLCAVST"
     gene            complement(404..>1562)
                     /gene="rpoN"
                     /locus_tag="PK077_11205"
     CDS             complement(404..>1562)
                     /gene="rpoN"
                     /locus_tag="PK077_11205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HRN24903.1"
                     /translation="EGRLSEDAAARRDFMYESVVAPESLKTHLMDQAQHSALTGRARD
                     ALFLLIDALDERGFLTESPQELEEQGCFSMRDMEEALAALREMDPPGVGAANLRDSLL
                     IQLEQRGLKRSLAFRLVKRCWRELAAHKYEEAARLLDVEPGAVAAALEVIRSLTPDPG
                     GAYAPGGNPHLLPDVIVEEGPSGVLEVILTSEYLPRLSMNERYMELMAEGSGSRELRQ
                     YLRRAFREGQELLRALDMRQETVLRLARVIVRRQEDFFRSGPSRLKAMGMEEVAEEMG
                     VHVSTVSRACRDKYLLCKWGMKELRSFFSAGVPSEGGVSRDGAASGAVAAGAVQELMR
                     RLIAEEDSSKPLSDAGLAAALREKGVNIARRTVAKYREQMKILPASLRRGI"
BASE COUNT          368 a          534 c          387 g          273 t
ORIGIN      
        1 gtggagactg cgcataaaag gcacgcatat tggtaacttc ctttgaaccc ggcccggcga
       61 atcacgccgc cagccagcag cgttccggcc aggttcccga gaaaaaagaa agcccccacc
      121 agccctgcct cccaggtggc ggcatcaaat tgctccatcc acaggggaac aaccgtattc
      181 aatacggcaa acgccactgt ctggaaaaga agccccgcca gcagcagggc aataggccag
      241 gtaaatgtac gcatcacgat aaagcgagga ctatagcacc aaagccgcat cagtcaaccg
      301 cctcttccgt cggaaagaaa aacaaaaaaa gcgccctatc aacaaaagac acaatacatt
      361 gattgttaaa ttattaataa acaattcaaa atatagggga ctgtcatatt ccccggcgca
      421 gggaagcggg cagtatcttc atctgctccc gatatttggc caccgtcctg cgggcgatgt
      481 tcaccccttt ttctcgcagc gcggccgcca gcccggcgtc actgaggggt ttggatgaat
      541 cttcctccgc aatcagccgc cgcatcagct cctggacggc gccggcagcc acggctccgg
      601 aagccgcccc gtcccgggaa acgccgcctt cagacggaac tcccgcactg aaaaaggatc
      661 tcagctcttt catcccccac ttgcacaaca ggtacttgtc ccggcacgcc cgggaaaccg
      721 tggaaacatg aacccccatc tcctccgcca cttcttccat gcccatggcc ttcaggcggg
      781 acgggccgga cctgaaaaaa tcctcctgcc tccgcacaat gacacgggcc aggcgcaaaa
      841 cggtctcctg cctcatatcc aaagcccgca acaactcctg tccctcacgg aacgccctgc
      901 ggagatattg cctcagctcc cggcttccgg aaccctcagc cattaactcc atataccgtt
      961 cattcatgga caggcgcgga agatactcgg aagtgaggat aacctccagc acgccggaag
     1021 gcccctcctc cacaatgacg tcaggcagca gatgagggtt tccgccgggg gcgtaggctc
     1081 cccctggatc aggagtcagg ctcctaatca cctccagagc cgccgccacg gcccccggtt
     1141 ccacgtccag caggcgtgcg gcttcctcat acttgtgcgc cgccagttcc cgccagcagc
     1201 gcttgaccag gcggaaagca agcgaacgct tcaaaccgcg ctgttcaagc tggatcagca
     1261 gggaatccct taaattcgcc gcccccactc cgggcgggtc catttccctc agcgcagcca
     1321 gagcttcctc catgtcccgc atgctgaaac acccctgctc ttccagctcc tgcggagact
     1381 ccgtcagaaa cccgcgttcg tccaaagcgt caatcagcag gaacagggcg tcccgggcgc
     1441 ggcccgtcaa tgcggaatgc tgcgcctgat ccatcaaatg ggttttcagg gattccggag
     1501 ccactacgga ttcatacatg aaatcacggc gtgcggccgc gtcctcagaa aggcggcctt
     1561 cc
//
LOCUS       DAOMSU010000410         1557 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_9012_length_1557_cov_2.627164, whole genome shotgun sequence.
ACCESSION   DAOMSU010000410 DAOMSU010000000
VERSION     DAOMSU010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9012_length_1557_cov_2.627164"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1405)
                     /locus_tag="PK077_11210"
     CDS             complement(<1..1405)
                     /locus_tag="PK077_11210"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HRN24904.1"
                     /translation="MLSSTLEDTLRLFGKERFRPMQRELVECALEGRSCIGILPTGSG
                     KSLCYQIPAVLGNGLTVVVSPLIALMRDQVAGLEKLGVAAARYDSTLAEEDKVSLMDR
                     LKSGGVRLLFAAPESLESPWIQQVMEFVPPGLFVVDEAHCLSEWGHSFRPDYLGLPVF
                     FRKHGFRSVMALTATATEQVCRDLAGLFGVRDECIFRAAPYRANIFRQVETLREQDKT
                     ARLVELLKEEGRRPAVVYTRTRKDAENLSYELGKAGFSVKSYHAGMPPETRGLVQDEF
                     LAGTADVLVATIAFGMGIDKPDVRSVVHYHPPASLEAYVQESGRAGRDGLPSFSLVML
                     SARDSVAVVNRLHAAEPDRHGMKGLVSLLSRRGEHIVSLYEASSVYDLPDVAVDRMLF
                     DLKRSGSVREKGTGFKYYRVRPLFRMEEILCGRGGEECARLQWMDMRRQGEVEDLAVE
                     WGISWEEAAAWLGDLALS"
BASE COUNT          367 a          536 c          392 g          262 t
ORIGIN      
        1 cggaaagagc caggtcacca agccaggcgg cggcttcttc ccatgaaatt ccccattcca
       61 cagccagatc ctccacctct ccctgccggc gcatatccat ccattgcagc cgggcgcatt
      121 cctccccgcc gcggccgcac agaatttcct ccatccggaa cagagggcgc accctgtaat
      181 acttgaaccc cgtccccttc tcccgtacgg aaccggaacg tttcaggtca aacagcatcc
      241 ggtccaccgc cacatccggc aaatcataaa cggaggaagc ctcatacagg gaaacaatgt
      301 gctcccccct gcgggacaac agggatacca gccctttcat gccgtgccgg tccggctccg
      361 ccgcatgcag ccggttgact acggccacgc tgtcgcgggc ggacaacatc accaggctga
      421 aggaaggaag gccgtcgcgt cccgcacggc cggattcctg aacatacgcc tccagactgg
      481 cggggggatg gtaatgcact acggaacgca cgtccggctt gtcaatcccc atgccgaagg
      541 caatcgtcgc caccagcacg tccgccgtac ctgccaaaaa ctcatcctga accagccccc
      601 tcgtctccgg gggcatgccc gcatggtagg acttgacgga gaaaccggct tttcccaatt
      661 cgtaggaaag gttttccgca tctttgcggg tccgcgtgta caccacggcg ggacgacggc
      721 cttcctcttt caacaattca accagccgcg cggtcttatc ctgctccctc aacgtttcca
      781 cctgacggaa aatattcgca cggtacggag cagcccggaa aatacattca tcgcgcacgc
      841 caaacaggcc ggccagatcg cggcacacct gctccgtagc cgtggccgtc aacgccatga
      901 cgctgcggaa cccgtgcttt ctgaaaaaaa cgggcagccc cagataatcc ggacggaaac
      961 tgtgccccca ttccgaaagg caatgagcct catccaccac gaacaagccg gggggaacaa
     1021 actccatgac ctgctgaatc cacggggatt ccagagactc cggagcggca aacagaaggc
     1081 ggacgcctcc ggacttcaat ctgtccatca gggagacttt atcttcttcc gctagggtcg
     1141 aatcatacct ggcggcggcc acgcccagtt tttccagccc tgccacctgg tcgcgcatca
     1201 gggcaatcaa cggagaaacc acaaccgtca gcccatttcc cagaaccgcc ggaatctgat
     1261 agcaaaggct cttgccggaa cccgtaggca aaataccgat gcaggaacgg ccctccagcg
     1321 cgcactccac aagctccctc tgcatgggac ggaaccgctc cttgccgaac aaacgcagcg
     1381 tgtcttccag agtagaggaa agcatggggc aagaatacca taacgcagaa tcccggcaaa
     1441 aggaatccta tgacattgac acggcggcgc catcctatat cctccaggca atatgagacc
     1501 cttgaaaacc atcgtcgccg gaactctggc cctgctggcg gcagctcccc tgtcagc
//
LOCUS       DAOMSU010000411         1551 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_9084_length_1551_cov_1.812834, whole genome shotgun sequence.
ACCESSION   DAOMSU010000411 DAOMSU010000000
VERSION     DAOMSU010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9084_length_1551_cov_1.812834"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PK077_11215"
     CDS             <1..418
                     /locus_tag="PK077_11215"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRN24905.1"
                     /translation="PENCILIENKSTNTGENVRFSREVLKRAGIGEPVSLIALQKPYM
                     ERRTLATLQAQWPEARVAVSSPPFTFMEYLTGELPQELVVSAMVGDFQRILEYPKQGF
                     STEQPVPPEAMAAFRMLVEAGYGSQLLKGVPLPWNS"
     gene            502..>1551
                     /locus_tag="PK077_11220"
     CDS             502..>1551
                     /locus_tag="PK077_11220"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRN24906.1"
                     /translation="MADRTNTDPARMEKIVSLCKRRGFIFQSAEIYGGLNGCWDYGPM
                     GVELKRNLKEYWWRKNVQEREDVVGMDGSILTHNSVLVASGHVGGFSDPMCDCLLTKE
                     RLRADQVPAQSGMGFFYTGAAKKDGSWNIEKKYSVLVESEKQADKARKTAAQYYAQLA
                     GKDASYKDMELKGECMETVTDSTMYNPANGSLLTEAREFNLMFKTTIGATSDENDPNA
                     TGWLRPETAQSIFCQYKNILDSSRVKLPFGIAQIGKSFRNEINPRNFTFRSREFEQME
                     IEYFCRPEDGLRLVDEWLEHRLCFYDEVGVPREHIHILDVPDGERAFYSKKTYDLEYE
                     FPFGIQELEGIAYRTD"
BASE COUNT          377 a          412 c          433 g          329 t
ORIGIN      
        1 tccggaaaac tgcatcctga tagaaaacaa atccaccaac accggggaaa atgtccgctt
       61 ctcacgggaa gtgctgaaac gggcgggaat cggggagcct gtcagtctca tcgccctgca
      121 aaagccctac atggaacggc ggacgctggc tacgctccag gcccagtggc cggaagcgcg
      181 ggtggcggtc agctcccctc ccttcacgtt catggaatac ctgactgggg aactgccgca
      241 ggagctggtt gtctccgcca tggtggggga ctttcaaagg atactggaat atcctaaaca
      301 gggattttcc acggaacagc ctgtgcctcc ggaggcgatg gcggcattcc gcatgctggt
      361 ggaagcgggg tatggctccc agctgctcaa gggtgtcccc cttccttgga attcttgaga
      421 agacacgctt tttgctttta ctgaatgctg attgcgggca tcaatacaac ccgcatagcc
      481 aacccaatta tataacttat catggcagac agaaccaaca cagaccccgc ccgaatggaa
      541 aaaatcgtga gcctctgtaa aaggagggga ttcatcttcc agtcagctga aatttacggc
      601 ggtctgaacg gttgctggga ctatggtccc atgggggtgg agctcaagcg caatctgaaa
      661 gaatactggt ggcgtaaaaa tgtgcaggaa cgcgaggatg tggtcggcat ggacggttcc
      721 atcctgacgc ataactccgt actggtggct tccggccatg tgggcggatt ctccgacccg
      781 atgtgcgatt gcctgttgac caaggagcgt ctgcgtgcgg accaggtccc ggcccagagc
      841 ggcatggggt ttttctatac cggagccgcc aaaaaagacg gttcctggaa tattgaaaag
      901 aagtactccg ttctggtgga atctgaaaaa caggcggaca aggcccgtaa gaccgcggct
      961 caatattatg cccagctggc cggcaaggat gcctcttaca aggatatgga gctgaaaggc
     1021 gagtgcatgg aaaccgtaac ggattccacc atgtacaatc ctgctaacgg ttctctgctg
     1081 acggaagccc gtgagttcaa cctcatgttc aagacgacca ttggagctac ctctgatgaa
     1141 aacgatccca acgctacggg ctggctgcgc ccggaaacgg cccagtccat tttctgccag
     1201 tataagaaca ttctggacag ttcccgcgtc aaactgcctt tcggaatcgc ccagatcgga
     1261 aaatctttcc gcaatgagat taatccgcgg aacttcactt tccgttcccg cgaattcgaa
     1321 caaatggaaa tcgaatattt ctgccggccg gaagacgggc tgcgcctggt ggatgaatgg
     1381 ctggaacacc gtctttgctt ctatgacgaa gtgggggttc cgcgcgaaca cattcatatt
     1441 ttggacgtgc cggacggcga acgcgctttc tactccaaga aaacctatga cctggagtat
     1501 gaattcccct tcggcatcca ggaactggag ggcatcgcct accggacgga t
//
LOCUS       DAOMSU010000412         1548 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_9118_length_1548_cov_1.896182, whole genome shotgun sequence.
ACCESSION   DAOMSU010000412 DAOMSU010000000
VERSION     DAOMSU010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9118_length_1548_cov_1.896182"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1548
                     /locus_tag="PK077_11225"
     CDS             <1..>1548
                     /locus_tag="PK077_11225"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRN24907.1"
                     /translation="GIRAASLGYFGKEPADLTTREAASIAALIKNPNGLSPLNNPASN
                     LKWRNHVLNRMAKEKYITPDEAERLSAMELGLNPKPLKRGVSHIYDRISSEITAYLGE
                     ERVNAAGLKIYTTIDRQLQEVTGKALEQALENIEKQPGYRHQKAADYHSGSANKPRYL
                     EGALLVVDNKSGAVLAYHGGRDYTKRQYDAIRDGARPTGTSILPFLYATAFDTGKSPV
                     TRILDDAIDNRLTGIGGSEGILGEWGAENSKNRYEGMITAHRGLSASKIAASLRMGME
                     MGTAPFVKKLTDFGIRKPVREAGSTEVNPVYRPKIFVGTEPASLKEMTLAYTAIPNGG
                     SRPQDIYYLDRIEDEDGQLIWESTQAIETRNNAQIRKGATSTATAFQLHNILNSSLKN
                     GSAQRVAPYLPKNFKGGVKTGTTYDFADNWLFGYDSRITCGIWVGFLEGKKPIYHGAF
                     SSDTCASVLGTAITEAERQFPAGELAPPPSVERVEICLTTGKRATHSCYDIDPSDKKY
                     RRHTIFEY"
BASE COUNT          384 a          489 c          416 g          259 t
ORIGIN      
        1 ggcatccggg ccgcttccct gggctacttc ggcaaagagc cggcggatct gaccacccgg
       61 gaggccgcct ccatcgccgc cctgatcaaa aacccgaacg gactttcccc tctgaataat
      121 ccggctagca atctgaaatg gcgcaaccac gttctcaacc gcatggccaa ggagaaatac
      181 attacgccgg atgaagcgga acgcctctcc gccatggagc tggggctgaa tcccaagccg
      241 ctgaaacgcg gcgtctccca catttacgac cgcatttcct cagaaatcac cgcatacctg
      301 ggggaggagc gcgtcaatgc cgccgggctt aaaatttaca ccaccattga caggcaactc
      361 caggaagtta ccggaaaagc cctggaacag gctctggaaa acattgaaaa acaacccggt
      421 taccgtcatc agaaggccgc agactaccac agcggttccg cgaacaagcc gcgctatctg
      481 gaaggagcgc tgctggtggt ggacaacaaa tcaggcgccg tcctggccta ccacggaggc
      541 agggattaca ccaagcgcca gtatgacgcc atcagggacg gagcccgccc caccggcacc
      601 tccatcctcc ctttcctgta cgctacagcc tttgacacgg gaaaaagccc tgtcacccgc
      661 attctggacg atgccatcga caaccgcctg acgggcatcg gcggttcgga aggaatcctg
      721 ggggaatggg gggcggaaaa ctccaaaaac cgttatgaag gcatgattac cgcccaccgc
      781 ggcctgtccg cctccaaaat cgccgcatcc ctgcgcatgg ggatggagat gggcacggcc
      841 ccgttcgtca agaagctgac ggatttcggc atccgcaagc ccgtgaggga agcgggcagc
      901 acggaagtaa atcccgttta ccgccccaaa attttcgtgg gcacggagcc tgcctccctg
      961 aaggaaatga cgctggccta cactgccatc ccgaacgggg gttcccgccc tcaggatatt
     1021 tactatctgg acaggataga agatgaagac ggccagctga tctgggaatc cacccaggcg
     1081 attgaaaccc ggaacaatgc ccagatacgg aagggggcca catccacagc cacggctttc
     1141 cagcttcaca acatcctgaa ttcttccctg aaaaacggtt cagcccagcg cgttgccccg
     1201 tatctgccga aaaacttcaa gggcggcgtg aaaacaggca ccacgtacga cttcgcggac
     1261 aactggctgt ttgggtatga cagccgcatt acctgcggca tctgggtagg gttcctggaa
     1321 ggcaaaaagc ccatttatca cggagccttc tcgtcagaca cctgcgcttc cgtgctgggc
     1381 accgccatca cggaagcgga acggcagttc cctgccggag aactggcccc tccccccagc
     1441 gtagaacgag tggaaatctg cctgaccacc gggaagaggg ccacgcacag ctgttatgac
     1501 attgacccgt cggataaaaa ataccgccga cacaccatat tcgaatac
//
LOCUS       DAOMSU010000413         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_9127_length_1547_cov_2.572386, whole genome shotgun sequence.
ACCESSION   DAOMSU010000413 DAOMSU010000000
VERSION     DAOMSU010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9127_length_1547_cov_2.572386"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..950
                     /locus_tag="PK077_11230"
     CDS             39..950
                     /locus_tag="PK077_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRN24908.1"
                     /translation="MWKDAFLRLSRNRAAMFSLLALALIAAACFLGPLLPWLPHPNVQ
                     DLSRIAESPSWDHWFGTDQLGRDLLARVLYGGRISLLVGVVATGVSLVIGVAYGLVSG
                     YAGGRLDALMMRLVDVLFALPFIVLVIIFSLSVEEPARRLTQWVSGMTGWSVEMVSPM
                     TGLIPLFIAIGALGWLTLARIVRTQTMELKGQEFVEAARSLGIGHMKILFRHIAPNLF
                     GAVIVYTTLAVPGIMLLEAVLSFLGLGVKAPNSSWGTLIKEGADRMEVSPELLLFPAL
                     LFSATLLALNFLGDGLRDALDPKSSKD"
     gene            969..1331
                     /locus_tag="PK077_11235"
     CDS             969..1331
                     /locus_tag="PK077_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HRN24909.1"
                     /translation="MKIALTISMLVVSNVFMTWAWYGHLKKGSGADGKPLLMIILMSW
                     GLAFFEYCFMIPANRLGSAGGLNVAQLKIMQEVITLCVFIPFALFYIGEKWKWDYLWA
                     FLCVLGSVFFVNRERLFG"
BASE COUNT          255 a          399 c          487 g          406 t
ORIGIN      
        1 aaagaggatg gaacaggacg gaggaacagg gttcctccct gtggaaggac gcttttctgc
       61 gtctttcccg caaccgggcg gccatgtttt ccctgctggc gctggcgctg attgcggcgg
      121 cctgttttct ggggcccctg ctgccgtggc tgcctcatcc taatgtgcag gatttgtccc
      181 ggattgcgga aagcccttcc tgggaccatt ggttcggcac ggaccagctt gggagggatc
      241 tgctggcgcg tgtcctgtac ggcggccgca tttccctgct ggtgggcgtg gtcgccacgg
      301 gcgtttccct ggtgattggc gtggcgtacg gactggtttc cggttatgcc ggaggcaggc
      361 tggatgccct gatgatgcgc ctggtggatg tgctttttgc cctgccgttc attgtgctgg
      421 tgattatttt ttccttgtcc gtggaggagc cggcccggag gctgacgcag tgggtttccg
      481 gcatgacggg ctggtctgtg gaaatggtga gccccatgac cgggctgatt cccctgttca
      541 tcgccatcgg cgctttgggc tggctgacgc tggcgcgcat tgtccggacg cagacgatgg
      601 agctgaaagg gcaggagttt gtggaagccg cccgctccct gggcatcggt cacatgaaga
      661 ttctgttccg ccatattgct ccgaatttgt tcggggccgt catcgtgtac acgactctgg
      721 ccgttcccgg catcatgttg ctggaagccg tcctgtcctt tctggggctg ggcgtgaagg
      781 ctcccaattc ctcatgggga acgctgatca aggaaggggc ggaccgcatg gaggtaagtc
      841 cggaattgct tttgttcccg gcgctcctgt tttcggctac cctgctggcg ctgaatttcc
      901 tgggggacgg cctgcgggac gccctggatc ccaagtcctc caaggactga tgtttttaag
      961 taaaaattat gaagattgcg ttaacgattt ccatgctggt ggtctccaac gtttttatga
     1021 cgtgggcgtg gtacggccat ctgaagaagg ggtccggggc ggatggcaag cccctgctga
     1081 tgattatcct gatgagctgg ggattggcgt tttttgaata ttgcttcatg attcccgcca
     1141 accgcctggg cagtgcgggc gggcttaacg tggcccagct caagattatg caggaggtga
     1201 ttaccctgtg cgtgtttatt cctttcgccc tgttctacat cggagaaaaa tggaagtggg
     1261 attacctgtg ggcgttttta tgtgtattgg gatccgtttt cttcgtcaac agggagcgtt
     1321 tgttcgggtg acagactttt tccgttcgca ttcctccctc cggactgtct taccctgaag
     1381 aaagatgaag atgaacataa ttcctgtttt tgccgcagca gtctcaatcg ttctgaccgc
     1441 aggctgccat catcgccacc acgatcgtga tgattaccgt tccgggccgc cgcgccatga
     1501 taattatcat catgaccgtg acggctggga gcaaaggcgg acaaagc
//
LOCUS       DAOMSU010000414         1547 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_9129_length_1547_cov_2.206434, whole genome shotgun sequence.
ACCESSION   DAOMSU010000414 DAOMSU010000000
VERSION     DAOMSU010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9129_length_1547_cov_2.206434"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>805)
                     /locus_tag="PK077_11240"
     CDS             complement(<1..>805)
                     /locus_tag="PK077_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HRN24910.1"
                     /translation="HAIPGNGWYNRNMCEIQFWKQSNKLVQGPRLWEKLRERYGSGFT
                     CAKLFWWYNMYSTADWTITPRPMYPADGRKIFDIYTQPMELRETIKKDLGEFPFPTFW
                     GPMAGIRSSQWIADSARWIERKHRPDLSLIYLPYLDYDLQKFGPSSTQAAHAAKAMDG
                     LLCDLIDFLEREGVTPVVLSEYGISDVSRSIALNRIFRERGWITVKPEMGTEMLDCGA
                     SRAFAVADHQTAHIYINDPSVKEEVKALLSATPGVEEIRETDFSGLSSAA"
     assembly_gap    806..815
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            1013..>1284
                     /locus_tag="PK077_11245"
     CDS             1013..>1284
                     /locus_tag="PK077_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRN24911.1"
                     /translation="MTASVMIPRMERQDKRLVDQLRPISFETGIAPNATASVLVTFGR
                     TKVICAVTIEEDVPRWMKVQRVEGGWLTAEYSMLPYSTLDRKRRDIT"
     assembly_gap    1285..1294
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1295..>1547
                     /locus_tag="PK077_11250"
     CDS             <1295..>1547
                     /locus_tag="PK077_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012419449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HRN24912.1"
                     /translation="AGKLDGRSSEIQRLIGRSLRAAVDLGKIGQRTIWVDCDVLQADG
                     GTRTASITGASVALAIAVNKLVAEGKLAESPMKRLVSAVS"
BASE COUNT          301 a          391 c          502 g          333 t
ORIGIN      
        1 agccgcggaa gaaaggccgg agaagtccgt ttccctgatt tcttccactc cgggtgtggc
       61 ggagagcagt gctttcactt cttcttttac ggaaggatca ttgatgtaga tatgggcagt
      121 ctggtggtcc gccacggcaa aggcgcggga ggcgccgcag tccagcattt ccgtacccat
      181 ttccggtttg acggtgatcc agccccgttc ccggaagatg cggttgaggg caatgctgcg
      241 ggatacgtcg gaaatgccgt attcactgag gacgacgggg gtgacgcctt cccgttccag
      301 aaagtcgatc aagtcgcaga gaagaccgtc catagccttt gccgcgtggg cagcctgggt
      361 cgaggacggt ccgaatttct gaaggtcata gtccagatag ggcagataga tgaggctgag
      421 gtcagggcgg tgtttccgtt ctatccaccg ggcggagtcc gctatccatt gggaggaccg
      481 gatccctgcc atggggcccc agaaggtggg gaaggggaat tctcccaggt cctttttaat
      541 ggtttcccgg agttccatgg gttgggtgta aatgtcgaag attttgcggc cgtccgccgg
      601 gtacatgggg cgcggcgtga tggtccagtc cgccgtggaa tacatattgt accaccagaa
      661 aagtttggcg caggtgaagc cggagccgta ccgttcacgc agtttctccc agaggcgcgg
      721 gccctgcacc agcttgttgg actgcttcca gaattggatt tcacacatgt tccggttgta
      781 ccatccgttg ccgggaatgg cgtgcnnnnn nnnnngcagg tgagggccgg aaaggccggg
      841 gggaaggagg aaacgctccg cccctccgcc caggcggaga gccgcggcat gtgttccatc
      901 atctggaggg aaagggccac cacgtcaatg acggccacac gggtgcgagg gtgtttcatg
      961 ggagggatat tagctgtttg gaaggggaat tccatctttc catttgaaaa agatgacggc
     1021 atccgtcatg attccccgca tggaacgtca ggataaacga cttgtggacc agttgcgtcc
     1081 gatcagcttt gaaacgggta ttgccccgaa tgccacggct tccgtgctgg tgactttcgg
     1141 acgcaccaag gtgatttgcg ccgttacgat tgaggaggat gtaccccgct ggatgaaggt
     1201 tcagcgtgtg gagggaggat ggctgacggc ggagtattcc atgcttccct attccacgct
     1261 tgaccgtaaa cgccgggata ttacnnnnnn nnnncggcgg ggaaactgga cggacgttcc
     1321 agcgagatcc agcgtctgat cggccgttct ctgagggcgg ccgtggatct gggcaagatt
     1381 ggccagcgca cgatttgggt ggactgcgac gtcttacagg ctgatggcgg tacccgcacc
     1441 gcttccatta ccggggcttc cgtagctctg gccattgccg tgaacaagct tgtcgcggaa
     1501 ggaaagctgg cggagtctcc catgaaaagg ctggtttccg ccgtatc
//
LOCUS       DAOMSU010000415         1535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_9221_length_1535_cov_2.837838, whole genome shotgun sequence.
ACCESSION   DAOMSU010000415 DAOMSU010000000
VERSION     DAOMSU010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9221_length_1535_cov_2.837838"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1081)
                     /locus_tag="PK077_11255"
     CDS             complement(254..1081)
                     /locus_tag="PK077_11255"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HRN24913.1"
                     /translation="MNALRRMPRHILFLRLAYQAGMCLLALVAVTLTAIDLTTEAAEW
                     EVTADRVIYWIFVLDYVVRFSISRSKWLFIKEHPWDLLAIIPFDALFRLFRFASLGEI
                     LRLARYLDLFSYAMRFTTRIRRFFNTNGFKYICIAALVIILLGAVGIHLAEGMSLPNG
                     IWWSFVTATTVGYGDTYPVTTSGKFLAVFLMLTGIGFVGTLTSTITSFFLSPHGGEAL
                     PYREEEIEAIKKKLDDLSSMTDEDIDTMAALLKTLRDASPAPSAGKEETPAIHSEKN"
     gene            complement(1165..>1535)
                     /locus_tag="PK077_11260"
     CDS             complement(1165..>1535)
                     /locus_tag="PK077_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HRN24914.1"
                     /translation="AAALEEWLKGLYPDKFSKVTLGKRLVSGAAVALQSGNDMGPEMR
                     AYMKAMGQEVPESHPQLELNPSNPLVKKLSALRTENPELAQMVADQIANTALLRAGML
                     DDPAVLAQSSQALMEQLLLK"
BASE COUNT          323 a          393 c          462 g          357 t
ORIGIN      
        1 ttcttatata tatttcaata ttgttgcaga tgtcaggagg cttcatgaca taggagagag
       61 cggatggttg gtgcttttag aactagttcc tttggccaac attgtcattg cggcatttct
      121 ttttttaaag gtagccaatc agctggtaat caacataaag acttaaaatc ttctcactga
      181 aaaacggttg agtatttggt atttgccctg ggacggacag ggcatttttt gatgtaatac
      241 caaacggatt ttgtcagttt ttttcggaat ggatagcagg cgtttcctct tttcccgctg
      301 aaggtgctgg ggaggcgtcc cgaagggttt tcagcagggc cgccatggtg tctatgtcct
      361 catccgtcat ggaggaaagg tcgtccagtt tctttttaat ggcttcaatt tcctcttccc
      421 ggtacggcag cgcttctccg ccgtggggac tcagaaagaa ggacgtgatg gtgctggtga
      481 gcgttcccac gaatccgatg cccgtgagca tcaggaatac agccagaaat ttgcctgaag
      541 tggtgaccgg ataggtatcc ccgtagccta cggtggtagc cgtgacgaag gaccaccaga
      601 tgccgttggg caggctcatg ccctccgcca gatggatgcc tacggccccc agcaggatga
      661 tgaccagggc ggcgatgcag atatacttga atccgttcgt gttgaagaaa cgccggatgc
      721 gcgtggtgaa cctcatggca taggaaaaca ggtccagata cctggcgagc cttaaaattt
      781 cccccagcga ggcgaacctg aacaggcgga acagggcgtc aaagggaata atggccagca
      841 aatcccaggg gtgttcctta atgaagagcc atttggaccg gctgatggag aagcggacca
      901 cataatccag cacaaaaatc cagtagatga cgcggtccgc cgtgacttcc cattccgccg
      961 cttccgtggt caggtcaatg gcggtcagcg ttacggcgac cagggccagc aggcacatgc
     1021 ccgcttgata ggccagcctc aggaacagaa tgtggcgggg catgcggcgt aacgcgttca
     1081 tggcgggaag agaaatgaaa aagccgcccc cggcggggtg ggcagggagc ggcttgggga
     1141 aagaggctgt ttcctccggg ccccttactt cagcaggagc tgttccatca gggcctggga
     1201 ggattgggcc agcacggcgg gatcgtccag catgccggcg cggagcaggg cggtattcgc
     1261 gatctggtcc gccaccattt gcgccagttc cgggttttcc gtccgtaggg cggaaagctt
     1321 tttcaccagc gggttggagg ggttcagttc cagctgcggg tggctttccg gcacttcctg
     1381 ccccatggct ttcatgtacg ccctcatttc cggccccatg tcattgccgc tttgcagggc
     1441 tacggcggct ccgctgacca ggcgcttgcc cagggtgact ttggagaatt tgtccgggta
     1501 cagccctttc agccattctt ccagggccgc ggctt
//
LOCUS       DAOMSU010000416         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_9281_length_1527_cov_2.125679, whole genome shotgun sequence.
ACCESSION   DAOMSU010000416 DAOMSU010000000
VERSION     DAOMSU010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9281_length_1527_cov_2.125679"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PK077_11265"
     CDS             complement(<1..298)
                     /locus_tag="PK077_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24915.1"
                     /translation="MANGAMRQIKRAPLRQHVAAAGDAAQFQRHGILLSALHAAGGKI
                     GTFRSKGVPVILPFSDRSVHAQGLRHGAGRRQTRFPVRGGGRNPHLLGIRQRTII"
     gene            549..>1527
                     /locus_tag="PK077_11270"
     CDS             549..>1527
                     /locus_tag="PK077_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24916.1"
                     /translation="MNSNTSSTGGERQDRPGFGWAGRSAALSLWIISVLLWCVLMRQS
                     FTAMPWDDMTVAGERLQELRRPFAAAGMAVGVLGSSLCLGCLFWLCLSSLTSSGWGIA
                     LRRVLAAGCWGFLPGVLVLAGTVGCLTTVIFPEAARYWGVADVASPGVQPFGVLDLAD
                     FSWWNPAWLYVRVGSIAVLSWSMTQVWETLVAEKNVWQPSFRRGSAAFCMVVTVMMLG
                     LLGMDMLGGTGKVVLSMFPVYMIAYVLLVSLAFAVLSAVFLRRLDGGKELPWPRLGGM
                     LTAMVLVKAYIVYSQYMITWYASIPAEMPFYNAAASSGWSGMFAAALVLQ"
BASE COUNT          249 a          410 c          498 g          370 t
ORIGIN      
        1 gaatgatcgt cctctggcgg atgcccagca ggtggggatt tcttccgccg ccccggaccg
       61 gaaaacgcgt ttggcgcctc cccgctccgt ggcgcagtcc ctgggcatga acggaacggt
      121 ccgaaaacgg gaggatgacc gggactcctt tgctgcggaa ggttcctatt tttcctccgg
      181 ccgcatgcag ggcggagagg aggattccat gcctctggaa ctgggcggca tctcccgcag
      241 ccgcgacgtg ctggcggagg ggcgcgcgct ttatctggcg cattgcgccg tttgccacgg
      301 agaggacgga agcggacgcg gaagcatggc cgcttatgac acctatccgc aaataggccc
      361 cttccgggat gaaaaatatg cttcctattc ttccggaaaa atgttcagga gcattcggtt
      421 ggggcaggga aacatgcccg ctttcggaaa cattctcacg gcgcggggaa tctggtgtct
      481 ggtggcgttt atccgtcatt tgcaagcgcc gccggaagaa cctgccgttc aacaaaggaa
      541 caacattcat gaacagcaat acatcttcaa caggcgggga acgccaggac cgtccgggat
      601 tcggctgggc cgggcgttcc gctgcccttt ccctgtggat catttccgtt ctattatggt
      661 gcgtgctgat gcgccagtcc tttacggcca tgccctggga cgacatgacg gtagccggag
      721 aaaggttgca ggaattgaga aggccgttcg ccgctgccgg aatggccgta ggggtccttg
      781 gctccagcct ctgtctgggg tgcctgttct ggctctgcct gtcttcactg acgagttccg
      841 gctggggcat tgcgctgaga agggtgctgg cggcgggttg ctgggggttt ttacccggtg
      901 ttttggtatt ggccggaacg gtcggctgcc tgactaccgt gatttttccg gaagcagccc
      961 gctattgggg agtggcggat gttgcctctc ccggcgtcca gcccttcggc gttctggatt
     1021 tggctgactt ctcatggtgg aacccggcat ggctgtacgt gcgcgtgggg agtattgcgg
     1081 tgctttcctg gagcatgacc caggtatggg aaacgctggt tgcggaaaaa aatgtctggc
     1141 agccttcctt ccgccggggc tctgccgcct tctgcatggt tgtgacggtg atgatgctgg
     1201 gcctgctggg gatggacatg ctgggaggga cgggaaaggt cgttctttcc atgttccccg
     1261 tatacatgat tgcatatgtt ctgctggtgt ccctggcatt cgctgtgttg tctgccgtgt
     1321 tcctgaggcg gctggacgga gggaaggagc tgccttggcc ccgcctgggc ggcatgctga
     1381 cggctatggt tctggtgaaa gcctatattg tgtattctca gtacatgatt acttggtacg
     1441 cctccattcc ggctgaaatg cccttttata acgcagccgc ttcctccggc tggtccggaa
     1501 tgtttgccgc ggctcttgtt cttcagc
//
LOCUS       DAOMSU010000417         1527 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Akkermansia muciniphila isolate AMR_MDS_5774
            NODE_9282_length_1527_cov_2.109375, whole genome shotgun sequence.
ACCESSION   DAOMSU010000417 DAOMSU010000000
VERSION     DAOMSU010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867995
            Sequence Read Archive: SRR1616987
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Akkermansia muciniphila (wastewater metagenome)
  ORGANISM  Akkermansia muciniphila
            Bacteria; Verrucomicrobiota; Verrucomicrobiia; Verrucomicrobiales;
            Akkermansiaceae; Akkermansia.
REFERENCE   1  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.175
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 20:59:00
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,256
            CDSs (total)                      :: 2,222
            Genes (coding)                    :: 2,205
            CDSs (with protein)               :: 2,205
            Genes (RNA)                       :: 34
            tRNAs                             :: 31
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 17
            CDSs (without protein)            :: 17
            Pseudo Genes (ambiguous residues) :: 0 of 17
            Pseudo Genes (frameshifted)       :: 5 of 17
            Pseudo Genes (incomplete)         :: 11 of 17
            Pseudo Genes (internal stop)      :: 3 of 17
            Pseudo Genes (multiple problems)  :: 2 of 17
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1527
                     /organism="Akkermansia muciniphila"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9282_length_1527_cov_2.109375"
                     /isolate="AMR_MDS_5774"
                     /isolation_source="Medicalfacilities Wastewater"
                     /db_xref="taxon:239935"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="48 N 8 E"
                     /collection_date="2012-11-15"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PK077_11275"
     CDS             complement(<1..215)
                     /locus_tag="PK077_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24917.1"
                     /translation="MQQGNKTTAPEIDFGEFAPATYAEWREAAVAALKGADFDKKLFT
                     KLVEGITLHPIYNKWDEHSPVMPAGQFP"
     gene            623..>1527
                     /locus_tag="PK077_11280"
     CDS             623..>1527
                     /locus_tag="PK077_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRN24918.1"
                     /translation="MWQRRTEAQLARLISRTDAALGDRLLGVIELDSEKHGRQHGSEK
                     LKEAAMEQVAREVSARDLTVNIPRPSHRKLFVLLAVLAACTAAVCAVSPEAAGNALKR
                     WVRPFNPPERYTFTQLAPTPDSLVIPMGESCLYEIRLAEGTKTRPQTAEYFFRNRVSQ
                     QAPLADGAYKIHIPPMQQADSLEFFAGDAVRRLNIIPKGRPSLLGAEAGVAYPAYMAR
                     ANGREAMRAGVISAPEGSTLTLKVSASQRLQSAEDAQGQPLRVDGNSVIIPNILLEKE
                     PREIELDWMDSDGLAASRPVKIQLEP"
BASE COUNT          330 a          452 c          441 g          304 t
ORIGIN      
        1 ggaaattgcc cggcaggcat taccggggaa tgttcatccc atttgttata gatgggatgc
       61 agggtaattc cctccacaag cttggtaaaa agcttcttgt caaaatcagc ccctttcagg
      121 gcagcgactg ctgcctcgcg ccattcggcg tatgtggctg gtgcgaattc tccgaaatca
      181 atttccggag ccgttgtttt atttccctgt tgcatggcga agtcaatggg taaggcctaa
      241 tatgttggtt tttaactatg cgcaaaccag gccaagttta aagcgcaaca ttattttagg
      301 aaatcaaagt gtaaagtcca gcgagaaaaa tcctcccgtc cgcctgcaaa tgcgaatttg
      361 cttcatcacg cggggcaaac ttgcaaaaac gatgtccatg gcgtaaatac gggaataagc
      421 atgccccctt ccactaccgt caaactccct ccccagctgg tcaggcagct ccgaactttc
      481 cggcgccgcc tcagaacggt gaagatgctg gaagccgtat gccttgcagg cattgccgtc
      541 attctttcct acgcgctcct gtacctttcc gacagattgt gggagacgcc tcccgccgtc
      601 ggctggctcc tgtttccggt gggtgtggca gaggcgcacg gaagcccagc tggcgcggct
      661 gatttcccgg acggacgcgg ccctggggga tcgtcttctg ggcgtcatcg aactggattc
      721 agaaaagcac ggccgccaac acggttccga aaagctgaaa gaggcggcca tggagcaggt
      781 ggcccgcgaa gtgtccgccc gggatctgac agtgaacatc ccccggccca gccacaggaa
      841 gctgttcgtc ctgctggcgg ttctcgccgc atgcacggcg gccgtatgtg cggtaagccc
      901 ggaagcggca gggaatgccc tgaaacgctg ggtccgcccg ttcaatcctc cggaacgcta
      961 tacattcacc cagctggccc ccaccccgga ttccctggtc attcccatgg gagagagctg
     1021 cctgtatgaa atccggctgg cggagggaac caaaacccgc ccgcagacgg cagagtattt
     1081 tttccgcaac agggtgagcc agcaagcgcc tcttgcggac ggcgcataca agatccacat
     1141 cccccccatg caacaggcgg acagcctgga gttctttgca ggggatgccg tgcgacggct
     1201 gaacatcatc ccgaaggggc gcccttccct gctgggagcc gaagccggcg tagcctatcc
     1261 tgcctatatg gcgcgcgcca acggcaggga agccatgaga gcaggagtta tttccgcccc
     1321 ggaaggctct acgctgacgt tgaaggtgtc cgcctcccag cgtctgcagt ctgcggagga
     1381 cgcgcagggc cagccgctcc gggtggacgg aaacagcgtc atcattccga acatcctttt
     1441 ggaaaaagag cccagggaaa tagagcttga ctggatggac agcgacgggc tggctgcttc
     1501 ccggcccgtg aagatacaac tggaacc
//
